Claims
- 1. A method of identifying a complex carbohydrate capable of binding an entity, the method comprising the steps of:
(a) producing an addressable combinatorial complex carbohydrate library by:
(i) providing a solid support having a plurality of locations; and (ii) enzymatically synthesizing a plurality of complex carbohydrate structures, each of said plurality of complex carbohydrate structures being attached to at least one addressed location of said plurality of locations, thereby producing the addressable combinatorial complex carbohydrate library; and (b) screening said addressable combinatorial complex carbohydrate library with the entity for identifying the complex carbohydrate capable of binding the entity.
- 2. The method of claim 1, wherein said entity is selected from the group consisting of proteins encoded by an EST library, and proteins extracted from a natural source.
- 3. The method of claim 1, wherein the entity is a candidate for a biologically active material, the method serves for identifying a complex carbohydrate which is a target for said candidate for a biologically active material.
- 4. The method of claim 1, wherein the entity is a ligand known to bind a specific natural complex carbohydrate and further wherein said addressable combinatorial complex carbohydrate library is a representation of domains of said specific natural complex carbohydrate, the method serves for identifying a specific domain of said domains which binds said ligand.
Parent Case Info
[0001] This is a divisional application of U.S. patent application Ser. No. 09/783,083, filed Feb. 15, 2001, which is a continuation-in-part of PCT/IL00/00099, filed Feb. 17, 2000, which claims priority from U.S. Patent Application No. 09/251,298, filed Feb. 17, 1999, now abandoned.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09783083 |
Feb 2001 |
US |
Child |
09860559 |
May 2001 |
US |