Claims
- 1. An isolated polynucleotide comprising a sequence selected from the group consisting of: consisting of: SEQ ID NO: 1-591, 1183-1912 and 1931-2106.
- 2. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) complements of a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; (b) reverse complements of a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; and (c) reverse sequences a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106.
- 3. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) nucleotide sequences having at least 75% identity to a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; (b) nucleotide sequences having at least 90% identity to a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; (c) nucleotide sequence having at least 95% identity to a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; (d) nucleotide sequences that hybridize to a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106 under stringent hybridization conditions; (e) nucleotide sequences that are 200-mers of a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; (f) nucleotide sequences that are 100-mers of a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; (g) nucleotide sequences that are 40-mers of a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; and (h) nucleotide sequences that are 20-mers of a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106; and (i) nucleotide sequences that are degeneratively equivalent to a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106, wherein the isolated polynucleotide encodes a transcription factor.
- 4. An oligonucleotide probe or primer comprising at least 10 contiguous residues complementary to 10 contiguous residues of a sequence of SEQ ID NO: 1-591, 1183-1912 and 1931-2106.
- 5. An isolated polypeptide encoded by a polynucleotide according to any one of claims 1-3.
- 6. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: SEQ ID NO: 592, 594-850, 852-930, 932-951, 953-1046, 1048-1182, 1913-1930, 2107-2293 and 2296-2368.
- 7. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
(a) sequences having at least 75% identity to a sequence of SEQ ID NO: 592, 594-850, 852-930, 932-951, 953-1046, 1048-1182, 1913-1930, 2107-2293 and 2296-2368; (b) sequences having at least 90% identity to a sequence of SEQ ID NO: 592, 594-850, 852-930, 932-951, 953-1046, 1048-1182, 1913-1930, 2107-2293 and 2296-2368; and (c) sequences having at least 95% identity to a sequence of SEQ ID NO: 592, 594-850, 852-930, 932-951, 953-1046, 1048-1182, 1913-1930, 2107-2293 and 2296-2368, wherein the isolated polypeptide functions as a transcription factor.
- 8. An isolated polynucleotide that encodes a polypeptide according to any one of claims 6 and 7.
- 9. A genetic construct comprising a polynucleotide according to any one of claims 1-3.
- 10. A transgenic cell comprising a genetic construct according to claim 9.
- 11. A genetic construct comprising, in the 5′-3′ direction:
(a) a gene promoter sequence; (b) a polynucleotide sequence comprising at least one of the following: (1) a polynucleotide coding for a polypeptide comprising a sequence of 592, 594-850, 852-930, 932-951, 953-1046, 1048-1182, 1913-1930, 2107-2293 and 2296-2368; and (2) a polynucleotide comprising a non-coding region of a polynucleotide of any one of claims 1-3; and (c) a gene termination sequence.
- 12. The genetic construct of claim 11 wherein the open reading frame is in a sense orientation.
- 13. The genetic construct of claim 11 wherein the open reading frame is in an antisense orientation.
- 14. A transgenic plant cell comprising a genetic construct of any one of claims 9 and 11.
- 15. A plant comprising a transgenic plant cell according to claim 14, or fruit or seeds or propagules thereof.
- 16. The plant of claim 15 wherein the plant is a woody plant.
- 17. The plant of claim 16 wherein the plant is selected from the group consisting of eucalyptus, pine, acacia, poplar, sweetgum, teak and mahogany species
- 18. A method for modifying gene expression in a plant comprising stably incorporating into the genome of the plant a genetic construct according to any one of claims 9 and 11.
- 19. The method of claim 18, wherein the plant is a woody plant.
- 20. The method of claim 18, wherein the plant is selected from the group consisting of eucalyptus, pine, acacia, poplar, sweetgum, teak and mahogany species.
- 21. A method for producing a plant having modified gene expression comprising:
(a) transforming a plant cell with a genetic construct according to any one of claims 9 and 11 to provide a transgenic cell; and (b) cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
- 22. The method of claim 21 wherein the plant is a woody plant.
- 23. The method of claim 21 wherein the plant is selected from the group consisting of eucalyptus, pine, acacia, poplar, sweetgum, teak and mahogany species.
- 24. A method for modifying the activity of a polypeptide in a plant comprising stably incorporating into the genome of the plant a genetic construct according to any one of claims 9 and 11.
- 25. The method of claim 24 wherein the plant is a woody plant.
- 26. The method of claim 24 wherein the plant is selected from the group consisting of eucalyptus, pine, acacia, poplar, sweetgum, teak and mahogany species.
- 27. An isolated polypeptide comprising a DNA-binding domain, wherein the DNA-binding domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 2279-2293 and 2296-2368.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US00/06112 |
Mar 2000 |
WO |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 09/640,211 filed Aug. 16, 2000, which claims priority to International Patent Application No. PCT/US/00/06112, filed Mar. 9, 2000, and to U.S. Provisional Patent Application No. 60/149,485, filed Aug. 18, 1999, and is a continuation-in-part of U.S. patent application Ser. No. 09/266,513, filed Mar. 11, 1999, now abandoned.
Provisional Applications (1)
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Number |
Date |
Country |
|
60149485 |
Aug 1999 |
US |
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
09640211 |
Aug 2000 |
US |
Child |
10856499 |
May 2004 |
US |
Parent |
09266513 |
Mar 1999 |
US |
Child |
09640211 |
Aug 2000 |
US |