Claims
- 1. An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence is at least 95% identical to SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
- 2. A vector comprising the isolated polynucleotide segment of claim 1.
- 3. An isolated host cell comprising the vector of claim 2.
- 4. The isolated polynucleotide segment of claim 1, wherein the polynucleotide sequence is at least 97% identical to SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
- 5. The isolated polynucleotide segment of claim 1, wherein the polynucleotide sequence is at least 99% identical to SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
- 6. An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence comprises SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
- 7. A vector comprising the isolated polynucleotide segment of claim 6.
- 8. An isolated host cell comprising the vector of claim 7.
- 9. A process for producing a polypeptide comprising culturing the host cell of claim 8 under conditions sufficient for the production of the polypeptide, wherein the polypeptide comprises SEQ ID NO:2.
- 10. The isolated polynucleotide segment of claim 6 encoding a fusion polypeptide, wherein the fusion polypeptide comprises SEQ ID NO:2.
- 11. An isolated polynucleotide segment comprising a polynucleotide sequence, wherein the polynucleotide sequence hybridizes to the full complement of SEQ ID NO:1, wherein the hybridization conditions include incubation at 42° C. in a solution comprising: 50% formamide, 5× SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1 × SSC at 65° C.; and, wherein the polynucleotide sequence is at least 95% identical to SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
- 12. The isolated polynucleotide segment of claim 11, wherein the first polynucleotide sequence is at least 97% identical to SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
- 13. An isolated polynucleotide segment comprising a polynucleotide sequence, wherein the polynucleotide sequence encodes a polypeptide comprising SEQ ID NO:2, and wherein the polynucleotide sequence is not genomic DNA.
- 14. A vector comprising the isolated polynucleotide segment of claim 13.
- 15. An isolated host cell comprising the vector of claim 14.
- 16. A process for producing a polypeptide comprising culturing the host cell of claim 15 under conditions sufficient for the production of the polypeptide, wherein the polypeptide comprises SEQ ID NO:2.
- 17. The isolated polynucleotide segment of claim 13 encoding a fusion polypeptide, wherein the fusion polypeptide comprises SEQ ID NO:2.
- 18. An isolated polynucleotide segment comprising a polynucleotide sequence, wherein the polynucleotide sequence encodes a polypeptide consisting of SEQ ID NO:2, and wherein the polynucleotide sequence is not genomic DNA.
- 19. A vector comprising the isolated polynucleotide segment of claim 18.
- 20. An isolated host cell comprising the vector of claim 19.
- 21. A process for producing a polypeptide comprising culturing the host cell of claim 20 under conditions sufficient for the production of the polypeptide, wherein the polypeptide consists of SEQ ID NO:2.
RELATED APPLICATIONS
This application claims benefit of U.S. Provisional Application No. 60/017,670, filed May 14,1996.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/US97/08272 |
|
WO |
00 |
3/5/1999 |
3/5/1999 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO97/43304 |
11/20/1997 |
WO |
A |
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
5804417 |
Martens et al. |
Sep 1998 |
|
5919664 |
Pearson |
Jul 1999 |
|
6165989 |
Black et al. |
Dec 2000 |
|
Foreign Referenced Citations (2)
Number |
Date |
Country |
881292 |
Dec 1998 |
EP |
97 43304 |
Nov 1997 |
WO |
Non-Patent Literature Citations (2)
Entry |
Gasc et al, J. Bacteriol. 173/22:7361-7367, Nov. 1991.* |
Rudinger, in Peptide Hormones, pp 5-7, 1976. |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/017670 |
May 1996 |
US |