Patent Grant
7052702
This application is a 371 of PCT/GB98/03001 filed Oct. 7, 1998, which claims the benefit of foreign priority under 35 U.S.C. §119(a)–119(d) to application United Kingdom 9721189.0 filed Oct. 8, 1997.
This invention relates to a class of novel agents that are able to modify nociceptive afferent function. The agents may inhibit the release of neurotransmitters from discrete populations of neurones and thereby reduce or preferably prevent the transmission of afferent pain signals from peripheral to central pain fibres. The agent may be used in or as a pharmaceutical for the treatment of pain, particularly chronic pain.
The sensation of pain due to injury or disease is carried from the periphery to the brain by a multi-neuronal pathway. The first part of this system comprises the primary nociceptive afferents that form synapses with secondary neurones in the dorsal horn of the spinal cord, or the nuclei of the cranial nerves. These synapses pass on the incoming information by the release of neurotransmitters and neuromodulators such as glutamate and substance P. These synapses are, therefore, possible sites for intervention to alleviate pain, indeed one of the modes of action of the opiate analgesics is to down-modulate neurotransmitter release at these synapses.
Unfortunately, the opiates have a number of limitations as drugs. Firstly, there are a number of chronic pain conditions for which the opiates are not effective. Secondly, the opiates have a number of side effects that are mediated both peripherally (constipation) and centrally (respiratory depression and euphoria) which present problems for long term use.
There is, therefore, a need for the development of new pharmaceuticals for the treatment of pain, particularly chronic pain.
One approach to this problem is the use of new agents containing fragments of clostridial neurotoxins (WO96/33273).
The clostridial neurotoxins are proteins with molecular masses of the order of 150 kDa. They are produced by various species of bacterium of the genus Clostridium, most importantly C. tetani and several strains of C. botulinum. There are at present eight different classes of the neurotoxins known: tetanus toxin, and botulinum neurotoxin in its serotypes A, B, C1, D, E, F and G, and they all share similar structures and modes of action. The clostridial neurotoxins are synthesised by the host bacterium as single polypeptides that are modified post-translationally to form two polypeptide chains joined together by a disulphide bond. The two chains are termed the heavy chain (H), which has a molecular mass of approximately 100 kDa, and the light chain (L), which has a molecular mass of approximately 50 kDa. Two distinct functions can be identified within the H-chain; binding and translocation. The carboxy-terminal half (HC) is involved in the high affinity, neurospecific binding of the toxin to cell surface acceptors, whilst the amino-terminal half (HN) is central to the translocation of the toxin into the neuronal cell. For botulinum neurotoxin type A these-domains are considered to reside within amino acid residues 872–1296 for the HC, amino acid residues 449–871 for the HN and residues 1–448 for the LC. The minimal domains necessary for the activity of the light chain of clostridial toxins are described in J. Biol. Chem. Vol.267, No.21, July 1992, pages 14721–14729. The eight distinct neurotoxin light chains (L) are highly specific zinc-dependent endopeptidases which each hydrolyse different but specific peptide bonds in one of three substrate proteins, synaptobrevin, syntaxin or SNAP-25. These substrates are important components of the neurosecretory machinery. The hydrolytic activity of the clostridial toxins results in a prolonged muscular paralysis. The functions of all three identified domains are necessary for the toxic activity of the clostridial endopeptidases.
Some of the clostridial endopeptidases, most notably botulinum neurotoxin type A, have been used as pharmaceutical agents for the treatment of a range of muscle dystonias. The flaccid paralysing action of the native botulinum toxins makes them appropriate for this use.
The use of fragments of clostridial neurotoxins for the desired purpose of analgesia is dependent on the invention of conjugates, or derivatives of these molecules, with a specific binding activity that will deliver the L-chain endopeptidase to the nociceptive afferent neurons in preference to other neurones in the relevant anatomical locus. Delivery of these conjugates includes binding to the cell surface, internalisation via an endosomal compartment and translocation of the clostridial endopeptidase activity into the cytosol.
Targeting of extracellular species to specific intracellular locations following endocytosis involves an appreciation of a number of possible targeting strategies. It is understood that early endosomes are part of the key sorting mechanisms of the cell, routing species to late endosome (and onto lysosomes for degradation), recycling to the cell surface or to the Trans-Golgi Network. Intracellular routing determinants have been suggested that determine the pathway and final destination of particular species (Mellman, 1996, Annu. Rev. Cell Biol., 12, 575–625).
Current data suggests that translocation of native clostridial neurotoxins occurs from an acidic intracellular compartment, though the exact location and nature of the compartment is unknown (Montecucco & Schiavo, 1994, Mol. Micro. 13, 1–8). In patent WO96/33273 it is proposed that for an agent to be effective, the agent must target to an appropriate compartment for translocation of the toxin. As an example of specific intracellular targeting, internalisation of the NGF-receptor is by specific endocytosis and retrograde routing (initiated by receptor-ligand complex), via acidic endosomes to the cell body, and an agent incorporating NGF is given in support of WO96/33273.
The present invention relates to an agent that can reduce and preferably prevent the transmission of pain signals from the periphery to the central nervous system, thereby alleviating the sensation of pain. Specifically, the invention can provide an agent that can reduce and preferably prevent the transmission of pain signals from nociceptive afferents to projection neurones. More specifically, the invention can provide an agent that can inhibit the exocytosis of at least one neurotransmitter or neuromodulator substance from at least one category of nociceptive afferents.
In one aspect of the invention, an agent is provided which can be administered to the spinal cord, and which can inhibit the release of at least one neurotransmitter or neuromodulator from the synaptic terminals of nociceptive afferents terminating in that region of the spinal cord.
In a second aspect of the invention, there is provided an agent which can specifically target defined populations of afferent neurones, so that the effect of the agent is limited to that cell type.
In a third aspect of the invention, there is provided a method of treatment of pain that comprises administering an effective dose of the agent according to the invention.
In a fourth aspect of the invention, the agent can be expressed recombinantly as a fusion protein that includes the required components of the agent.
Definitions
Without wishing to be limited by the definitions set down, it is intended in this description that the following terms have the following meanings:
Light chain means the smaller of the two polypeptide components of any of the clostridial neurotoxins. It is commonly referred to as the L-chain or simply L. An L-chain has a molecular mass of approximately 50 kDa, and it is a metalloprotease exhibiting high substrate specificity for vesicle and/or plasma membrane associated proteins involved in the exocytotic process.
Heavy chain means the larger of the two polypeptide components of any of the clostridial neurotoxins. It is commonly referred to as H-chain or simply H and has a molecular mass of approximately 100 kDa.
HC fragment means a peptide derived from the H-chain of a clostridial neurotoxin which is responsible for binding of the native holotoxin to cell surface acceptor(s) involved in the intoxicating action of clostridial toxin prior to internalisation of the toxin into the cell. It may be approximately equivalent to the carboxy-terminal half of the H-chain, or the domain corresponding to that fragment in the intact H-chain.
HN fragment means a fragment derived from the H-chain of a clostridial neurotoxin approximately equivalent to the amino-terminal half of the H-chain, or the domain corresponding to that fragment in the intact H-chain. It is characterised as:
LHN means a fragment derived from a clostridial neurotoxin that contains the L-chain, or a functional fragment thereof, coupled to a HN fragment.
BoNT/A means botulinum neurotoxin serotype A, and is a neurotoxin produced by Clostridium botulinum; it has a molecular mass of approximately 150 kDa.
LHN/A is LHN that is derived from Clostridium botulinum neurotoxin type A.
Targeting Moiety (TM) means any chemical structure of an agent which functionally interacts with a binding site causing a physical association between the agent and the surface of a primary sensory afferent.
Primary sensory afferent is a nerve cell that can carry sensory information from the periphery towards the central nervous system.
Primary nociceptive afferent is a nerve cell that can carry sensory information from the periphery towards the central nervous system, where that information can result in a sensation of pain.
Lectin is any protein that binds to oligosaccharide structures.
Galactose-binding lectin is a lectin that binds to oligosaccharide structures in which the terminal residue is derived from galactose or N-acetylgalactosamine.
It can be seen from this disclosure that an agent for reducing or preventing the transmission of pain signals from peripheral, nociceptive afferent neurones to projection neurones has many potential applications in the reduction of the sensation of pain, particularly of severe chronic pain.
Lectins are a class of proteins, often glycoproteins, that bind to carbohydrate structures. Lectins are found across the whole range of life forms from viruses to mammals. The most commonly exploited sources are the abundant lectins found in the seeds of plants. Lectins have previously been labelled and used as cell surface markers.
According to the invention, there is provided an agent that can inhibit the release of at least one neurotransmitter or neuromodulator or both from the synaptic terminals of nociceptive afferents.
It is known that such an agent can be produced based on the use of fragments of clostridial neurotoxin conjugated to a targeting ligand (WO96/33273). Given the known complexity of intracellular transport and the constraints on construct requirements, it is surprising that conjugates between toxin fragments and a specific sub-class of lectins that bind only to galactosyl residues form agents to produce analgesics that are particularly potent and selective. Inventions incorporating such lectins are the subject of this disclosure and several examples are provided.
One example of a class of plant-derived, galactose-binding lectins are those that can be purified from the seeds of the genus Erythrina. These lectins have been characterised to exist predominantly as non-covalent dimeric proteins with total molecular weights of approximately 60 kDa. Lectins have been isolated from several Erythrina species including: E. corallodendron (Gilboa-Garber and Mizrahi, 1981, Can. J. Biochem. 59, 315–320), E. cristagalli (Iglesias et al., 1982, Eur. J. Biochem. 123, 247–252), E. indica (Horejsi et al., 1980, Biochim. Biophys. Acta 623, 439–448), E. arborescens, E suberosa, E. lithosperma (Bhattacharyya et al., 1981, Archiv. Biochem. Biophys. 211, 459–470) E. caffra, E. flabelliformis, E. latissima, E. lysistemon, E. humeana, E. perrieri, E. stricta, and E. zeyheri (Lis et al., 1985, Phytochem. 24, 2803–2809).
These lectins have been analysed for their selectivity for saccharide binding (see e.g. Kaladas et al., 1982, Archiv. Biochem. Biophys. 217, 624–637). They have been found to bind preferentially to oligosaccharides with a terminal β-D-galactosyl residue.
A second example of a plant-derived, galactose-binding lectin with the desired binding specificity can be obtained from Glycine max (soy) beans. This lectin (soya bean agglutinin, SBA) is a tetrameric protein with a total molecular weight of approximately 110 kDa. It binds to oligosaccharides containing galactose or N-acetylgalactosamine residues.
An example of a galactose-binding lectin from bacteria is PA-I, obtained from Pseudomonas aeruginosa. PA-I is a D-galactosephilic lectin with a molecular weight of about 13 kDa and it binds to galactose-containing oligosaccharides (Gilboa-Garber and Mizrahi, 1981, Can. J. Biochem. 59, 315–320).
These and other lectins of the sub-class of galactose-binding lectins can be used as targeting moieties (TM) for conjugates of the type described in WO96/33273. The requirements for TMs in these agents are that they show specificity for the primary sensory afferents over other spinal nerves and that they lead to the internalisation of the agents into an appropriate intracellular compartment. The lectins of this invention fulfil these criteria. Surprisingly, in comparison to other lectins of WO96/33273, they can fulfil these criteria more efficiently and can provide agents with enhanced selectivity for nociceptive afferent neurosecretion.
Thus, in one embodiment of the invention a galactose-binding lectin is conjugated, using linkages that may include one or more spacer regions, to a derivative of the clostridial neurotoxins.
In another embodiment of the invention the agent is expressed in a recombinant form as a fusion protein. The fusion protein may be derived from nucleic acid encoding an appropriate fragment of a galactose-binding lectin, in addition to any desired spacer domains, with nucleic acid encoding all or part of a polypeptide of one serotype of neurotoxin. Such a nucleic acid may be a chimera derived from the nucleic acid encoding polypeptides from more than one serotype.
In another embodiment of the invention the required LHN, which may be a hybrid of an L and HN from different clostridial toxin serotypes, is expressed as a recombinant fusion protein with the galactose-binding lectin, and may also include one or more spacer regions.
In a further embodiment of the invention the required TM, L or LHN and translocation domain components may be separately expressed in a recombinant form and subsequently linked, covalently or non-covalently, to provide the desired agent.
In a further embodiment of the invention the required translocation domain may be of a non-clostridial origin, comprising instead a peptide or other entity capable of similar or enhanced function. Examples would include, but not be restricted to, the translocation domain of diphtheria toxin (O'Keefe et al., Proc. Natl. Acad. Sci. USA (1992) 89, 6202–6206; Silverman et al., J. Biol. Chem. (1993) 269, 22524–22532), the translocation domain of Pseudomonas exotoxin type A (Prior et al. Biochemistry (1992) 31, 3555–3559), the translocation domains of anthrax toxin (Blanke et al. Proc. Natl. Acad. Sci. USA (1996) 93, 8437–8442) and a variety of fusogenic or hydrophobic peptides of translocating function (Plank et al. J. Biol. Chem. (1994) 269, 12918–12924).
Exploitation in Industry
The agent described in this invention can be used in vivo, either directly or as a pharmaceutically acceptable salt, for treatment of pain.
For example, an agent according to the invention can be administered by spinal injection (epidural or intrathecal) at the level of the spinal segment involved in the innervation of an affected organ for the treatment of pain. This is, for example, applicable in the treatment of deep tissue pain, such as chronic malignant pain.
The present invention will now be described by reference to the following examples together with the Figures that show the following:
Materials
Lectin from E. cristagalli (ExL) was obtained from Sigma Ltd.
LHN/A was prepared essentially by the method of Shone C. C., Hambleton, P., and Melling, J. 1987, Eur. J. Biochem. 167, 175–180.
SPDP was from Pierce Chemical Co.
PD-10 desalting columns were from Pharmacia.
Dimethylsulphoxide (DMSO) was kept anhydrous by storage over a molecular sieve.
Denaturing sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed using gels and reagents from Novex
Immobilised lactose-agarose was obtained from Sigma Ltd.
Additional reagents were obtained from Sigma Ltd.
Methods
The lyophilised lectin was rehydrated in phosphate buffered saline (PBS) to a final concentration of 10 mg/ml. Aliquots of this solution were stored at −20° C. until use.
The ExL was reacted with an equal concentration of SPDP by the addition of a 10 mM stock solution of SPDP in DMSO with mixing. After one hour at room temperature the reaction was terminated by desalting into PBS over a PD-10 column.
The thiopyridone leaving group was removed from the product by reduction with dithiothreitol (DTT, 5 mM, 30 min). The product of this reaction was analysed spectrophotometrically at 280 nm and 343 nm to determine the degree of derivatisation achieved. The degree of derivatisation achieved was 0.8±0.06 mol/mol. The thiopyridone and DTT were removed by once again desalting into PBS over a PD-10 column.
The LHN/A was desalted into PBSE (PBS containing 1 mM EDTA). The resulting solution (0.5–1.0 mg/ml) was reacted with a four- or five-fold molar excess of SPDP by addition of a 10 mM stock solution of SPDP in DMSO. After 3 h at room temperature the reaction was terminated by desalting over a PD-10 column into PBS.
A portion of the derivatised LHN/A was removed from the solution and reduced with DTT (5 mM, 30 min). This sample was analysed spectrophotometrically at 280 mm and 343 nm to determine the degree of derivatisation. The degree of derivatisation achieved was 2.26±0.10 mol/mol. The bulk of the derivatised LHN/A and the derivatised ExL were mixed in proportions such that the ExL was in greater than three-fold molar excess. The conjugation reaction was allowed to proceed for >16 h at 4° C.
The product mixture was centrifuged to clear any precipitate that had developed. The supernatant was concentrated by centrifugation through concentrators (with 10000–50000 molecular weight exclusion limit) prior to a two step purification strategy. As the first step, the concentrated material was applied to a Superose 12 column on an FPLC chromatography system (Pharmacia). The column was eluted with PBS and the elution profile followed at 280 nm.
Fractions were analysed by SDS-PAGE on 4–20% polyacrylamide gradient gels, followed by staining with Coomassie Blue. The major band of conjugate has an apparent molecular mass of between 130–160 kDa; this is separated from the bulk of the remaining unconjugated LHN/A and more completely from the unconjugated ExL. Fractions containing conjugate were pooled prior to the second chromatography step; immobilised lactose-agarose. Selected post-Superose-12 fractions were applied to PBS-washed lactose-agarose and incubated for 2 hours at 4° C. to facilitate binding. Lectin-containing proteins (i.e. ExL-LHN/A conjugate) remained bound to the agarose during subsequent washing with PBS to remove contaminants (predominantly unconjugated LHN/A). ExL-LHN/A conjugate was eluted from the column by the addition of 0.3 M lactose (in PBS) and the elution profile followed at 280 nm. The fractions containing conjugate were pooled, dialysed against PBS, and stored at 4° C. until use.
In
On the SDS-PAGE gel there are bands due to lectin alone in fractions containing the conjugate, this material is probably due to the non-covalent homo-dimeric nature of the ExL; where only one monomer of ExL is covalently attached to the LHN/A the other is dissociated from the complex by the SDS in the electrophoretic procedure giving rise to these bands. The absence of free lectin monomers was confirmed by native PAGE analysis and is illustrated in
The procedure for production of a conjugate between a lectin from Erythrina corallodendron and LHN/A is essentially as described in Example 1 but with the following differences:
Materials
Lectin from E. corallodendron (EcL) was obtained from Sigma Ltd.
The procedure for production of a conjugate between a lectin from Glycine max_and LHN/A is essentially as described in Example 1 but with the following differences:
Materials
Lectin from G. max (SBA) was obtained from Sigma Ltd.
Method
For the affinity chromatography step an immobilised N-acetylgalactosamine (GalNAc) column was used and specific SBA-LHN/A was eluted by the addition of 0.3 M lactose.
The absence of free lectin monomers was confirmed by native non-denaturing PAGE analysis as illustrated in
The dorsal root ganglia contain the cell bodies of primary nociceptive afferent neurons. It is well established that in primary in vitro cultures of this tissue the neurons retain many of the characteristics of the nociceptive afferent. These characteristics include the ability to release neuropeptides such as substance P in response to chemical stimuli known to cause pain in vivo (e.g. capsaicin). Neurons anatomically adjacent to those of the DRG include those of the spinal cord. Cultures of SC neurons prepared from embryonic rats can be established in vitro and the release of neurotransmitter (3H-glycine) under potassium stimulation can be assessed. As such, the eSC neurons represent a model cell for testing the selectivity of the agents described.
The selectivity of the ExL-LHN/A agent for eDRG over eSC neurons is clearly illustrated in
Materials
Substance P enzyme linked immunosorbent assay kits were from Cayman Chemical Company.
Western blot reagents were obtained from Novex
Monoclonal antibody SMI-81 was from Sternberger Monoclonals Inc.
Methods
Primary cultures of dorsal root ganglion and embryonic spinal cord neurons were established following dissociation of the ganglia dissected from rat embryos (embryological age 12–15 days) For the preparation of eDRG neurons, the cells were plated into 12 well plates at an initial density of 3×105 cells/well in a medium containing NGF (100 ng/ml). After one day in culture, fresh medium containing cytosine arabinoside (10×10−6 M) was added to kill non-neuronal cells. After 2–4 days the cytosine arabinoside was removed. After several more days in culture the medium was replaced with fresh medium containing conjugate or LHN.
For the preparation of eSC neurons, Cells were plated onto poly-D-lysine coated 12 well plates (Costar) at a density of 2×106 cells per well (1 ml/well). ‘Plating’ medium was MEM with Earles Salts (Sigma), containing 5% foetal bovine serum (FBS), 5% heat inactivated horse serum (HS), 0.6% dextrose, 1.5 g/l NaHCO3 and 2 mM L-glutamine. Cultures are incubated at 37° C. with 10% CO2. The medium was changed to ‘feeding’ medium (plating medium minus the FBS with N1 (Sigma) 1/50 supplement) after one day. When glial cells became almost confluent anti-mitotic agents (15 microgrammes/ml 5-fluoro-2′-deoxyuridine (FdU) and 35 microgrammes/ml uridine (U)) were added for a further 2–3 days. Cells were cultured for at least 3 weeks prior to use.
The cells were incubated with these agents for varying times and then tested for their ability to release the neurotransmitters glutamate and substance P (eDRG) or glycine (eSC). After the release assays were performed the cells were lysed and the hydrophobic proteins were extracted by phase partitioning with Triton-X-114 following the method outlined in Boyd, Duggan, Shone and Foster (J. Biol. Chem. 270, 18216–18218, 1995).
Substance P Release Assay
The release of endogenous substance P was effected by collecting cell supernatants after treating the cells for 5 min with either a physiological balanced salt solution or a balanced salt solution in which the potassium ion concentration had been raised to 100 mM with consequent reduction in the sodium ion concentration to maintain isotonicity. Total substance P was measured after extraction in 2 M acetic acid, 0.1% trifluoroacetic acid and subsequent dehydration. Substance P immunoreactivity was measured using an enzyme immunoassay kit (Cayman Chemical Company).
[3H]Glutamate Release Assay
The release of glutamate was measured after loading the cells with [3H]glutamine as a radiotracer. The [3H]glutamine is converted to [3H]glutamate in the cell, and it is this [3H]glutamate that is taken up by synaptic vesicles and released upon depolarisation of the neuron. The cells are loaded with the [3H]glutamine (5×10−6 Ci/ml in HEPES-buffered MEM) for 2 h, then washed twice with HEPES-buffered MEM and thrice with balanced salt solution (BSS). Basal release was assessed with a 3 min incubation with BSS. Stimulated release was determined by a 3 min incubation with BSS in which the potassium concentration had been elevated to 80–100 mM with a consequent reduction in the sodium concentration to maintain isotonicity. All manipulations were performed at 37° C. The cells were lysed by the addition of Triton-X-100 (0.1%, v/v). For the basal and stimulated release superfusates the glutamate was separated from the glutamine by ion-exchange chromatography over Dowex-1 resin. The relevant fractions were analysed for 3H content by liquid scintillation counting.
[3H]Glycine Release Assay
The release of glycine was measured after loading the cells with [3H]glycine as a radiotracer. The [3H]glycine is taken up by synaptic vesicles and released upon depolarisation of the neuron. The cells are loaded with the [3H]glycine (2×10−6 Ci/ml in HEPES-buffered MEM) for 2 h, then washed once with HEPES-buffered MEM and thrice with balanced salt solution (BSS). Basal release was assessed with a 5 min incubation with BSS. Stimulated release was determined by a 5 min incubation with BSS in which the potassium concentration had been elevated to 56 mM with a consequent reduction in the sodium concentration to maintain isotonicity. All manipulations were performed at 37° C. The cells were lysed by the addition of 2 M acetic acid, 0.1% trifluoroacetic acid. Fractions were analysed for their 3H content by liquid scintillation counting and inhibition of release determined.
Western Blotting
ExL-LHN/A was applied to eDRG for 16 hours. After the determination of neurotransmitter release the cells were lysed by the addition of 2 M acetic acid, 0.1% trifluoroacetic acid and subsequently dehydrated. To extract the membrane proteins from these mixtures Triton-X-114 (10%, v/v) was added and incubated at 4° C. for 60 min, the insoluble material was removed by centrifugation and the supernatants were then warmed to 37° C. for 30 min. The resulting two phases were separated by centrifugation and the upper phase discarded. The proteins in the lower phase were precipitated with chloroform/methanol for analysis by Western blotting.
The extracted protein samples were applied to 4–20% polyacrylamide gradient gels and subjected to electrophoresis prior to transfer to nitrocellulose. Proteolysis of SNAP-25, a crucial component of the neurosecretory process and the substrate for the zinc-dependent endopeptidase activity of BoNT/A, was then detected by probing with an antibody (SMI-81) that recognises both the intact and cleaved forms of SNAP-25 (
Using methodology described in Example 4, the activity of SBA-LHN/A in primary neuronal cultures was assessed. The selectivity of the SBA-LHN/A conjugate for eDRG over eSC neurons is illustrated in
Using methodology described in Example 4, the activity of WGA-LHN/A in primary neuronal cultures was assessed. WGA represents an example of a non-galactosyl targeted lectin and therefore serves as an indicator of the properties of conjugate that do not recognise galactosyl moieties. The lack of selectivity of the WGA-LHN/A conjugate for eDRG over eSC neurons is illustrated in
A dose of 45 microgrammes of ExL-LHN/A in a 10 microlitres volume of vehicle was given by intrathecal injection to rats between lumbar sections L4–L5, 24 hours prior to electrophysiological analysis of neuronal activity. Animals were allowed to recover and movement was not restricted prior to sacrifice and analysis. The results from a group of 3 animals with 10 neurons recorded per animal, show that there was a 73% reduction in the C-fibre responses of the neurones (
In an accepted in vivo model of pain, the mouse hotplate test, ExL-LHN/A has been demonstrated to exhibit analgesic properties.
Materials
Adult outbred mice (MF1) of either sex, weight range 20 to 30 g.
Methods
Test material is injected into the intrathecal space of anaesthetised mice using a 30 gauge disposable needle attached to a 50 microlitre Hamilton syringe. The site of injection was normally chosen to be between lumbar vertebrae 5 and 6. The needle is inserted into the tissue to one side of the vertebrae so that it slips into the groove between the spinous and transverse processes. The needle is then moved carefully forward to the intervertebral space. 5 microlitres of test material is then injected into the intrathecal space and the needle withdrawn. The skin incision is then closed with a single wound clip and the animal placed in a box to allow recovery.
| Number | Date | Country | Kind |
|---|---|---|---|
| 9721189 | Oct 1997 | GB | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/GB98/03001 | 10/7/1998 | WO | 00 | 6/22/2000 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO99/17806 | 4/15/1999 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 4792447 | Uhr et al. | Dec 1988 | A |
| 4873346 | Anderson | Oct 1989 | A |
| 5433946 | Allen, Jr. et al. | Jul 1995 | A |
| 5668255 | Murphy | Sep 1997 | A |
| 5721207 | Nobel et al. | Feb 1998 | A |
| 5989545 | Foster et al. | Nov 1999 | A |
| 6235313 | Mathiowitz et al. | May 2001 | B1 |
| 6395513 | Foster et al. | May 2002 | B1 |
| 6461617 | Shone et al. | Oct 2002 | B1 |
| 6632440 | Quinn et al. | Oct 2003 | B1 |
| 20030049264 | Foster et al. | Mar 2003 | A1 |
| 20030147895 | Shone et al. | Aug 2003 | A1 |
| 20030166238 | Shone et al. | Sep 2003 | A1 |
| 20040071736 | Quinn et al. | Apr 2004 | A1 |
| Number | Date | Country |
|---|---|---|
| 197 35 105 | Mar 1999 | DE |
| 0 602 686 | Jun 1994 | EP |
| 0 673 938 | Sep 1995 | EP |
| WO 9109871 | Jul 1991 | WO |
| WO 9215327 | Sep 1992 | WO |
| WO 9304191 | Mar 1993 | WO |
| WO 9315766 | Aug 1993 | WO |
| WO 9421300 | Sep 1994 | WO |
| WO 9428923 | Dec 1994 | WO |
| WO 9517904 | Jul 1995 | WO |
| WO 9528171 | Oct 1995 | WO |
| WO 9532738 | Dec 1995 | WO |
| WO 9533850 | Dec 1995 | WO |
| WO 9612802 | May 1996 | WO |
| WO 9633273 | Oct 1996 | WO |
| WO 9713410 | Apr 1997 | WO |
| WO 9718790 | May 1997 | WO |
| WO 9807684 | Feb 1998 | WO |
| WO 9807864 | Feb 1998 | WO |
| WO 9808540 | Mar 1998 | WO |
| WO 9958571 | Nov 1999 | WO |
| WO 0004926 | Feb 2000 | WO |
| WO 0010598 | Mar 2000 | WO |
| WO 0057897 | Oct 2000 | WO |
