Claims
- 1. DNA having less than 10% of the base pairs of a yeast chromosome, having the same DNA sequence capable of replication and stable mitotic maintenance in a yeast host comprising a DNA segment coding for centromere-like activity during mitosis of said yeast host and a DNA sequence coding for a replication site recognized by said yeast host.
- 2. DNA according to claim 1, wherein said DNA segment coding for centromere-like activity is fewer than 2kbp.
- 3. A yeast host containing a DNA fragment derived from a yeast chromosome having centromere-like activity, having fewer than 10% of the base pairs of a yeast chromosome having the same DNA sequence and imparting mitotic stability joined to an ars gene.
Parent Case Info
This is a continuation of pending patent application Ser. No. 185,453, now abandoned, filed Sept. 9, 1980.
Government Interests
The invention described herein was made in the course of or under a grant from the National Cancer Institute, National Institute of Health (Grant No. CA-11034).
Non-Patent Literature Citations (5)
Entry |
Ruddle et al.: Nature 294, 115, (1981). |
Lewin: Gene Expression, vol. 2, second edition, John Wiley & Sons, New York, 1980, pp. 242-252. |
Klobutcher et al.: Nature 280, 657, (1979). |
Hinnen et al.: Proc. Natl. Acad. Sci., USA: 75, 1929, (1978). |
Struhl et al.: Proc. Natl. Acad. Sci., USA: 76, 1035, (1979). |
Continuations (1)
|
Number |
Date |
Country |
Parent |
185453 |
Sep 1980 |
|