Mutation or genetic engineering DNA or RNA concerning genetic engineering, vectors

CCHEMISTRY METALLURGY C12Biochemistry C12NMICRO-ORGANISMS OR ENZYMES COMPOSITIONS THEREOF PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS MUTATION OR GENETIC ENGINEERING CULTURE MEDIA

C12N15/00Mutation or genetic engineering DNA or RNA concerning genetic engineering, vectors

C12N15/01Preparation of mutants without inserting foreign genetic material therein Screening processes therefor C12N15/02Preparation of hybrid cells by fusion of two or more cells C12N15/03Bacteria C12N15/04Fungi C12N15/09Recombinant DNA-technology C12N15/10Processes for the isolation, preparation or purification of DNA or RNA C12N15/1003Extracting or separating nucleic acids from biological samples C12N15/1006by means of a solid support carrier C12N15/101by chromatography C12N15/1013by using magnetic beads C12N15/1017by filtration C12N15/102Mutagenizing nucleic acids C12N15/1024In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material C12N15/1027by DNA shuffling C12N15/1031mutagenesis by gene assembly C12N15/1034Isolating an individual clone by screening libraries C12N15/1037Screening libraries presented on the surface of microorganisms C12N15/1041Ribosome/Polysome display C12N15/1044Preparation or screening of libraries displayed on scaffold proteins s C12N15/1048SELEX C12N15/1051Gene trapping C12N15/1055Protein x Protein interaction C12N15/1058Directional evolution of libraries C12N15/1062mRNA-Display C12N15/1065Preparation or screening of tagged libraries C12N15/1068Template (nucleic acid) mediated chemical library synthesis C12N15/1072Differential gene expression library synthesis C12N15/1075by coupling phenotype to genotype, not provided for in other groups of this subclass C12N15/1079Screening libraries by altering the phenotype or phenotypic trait of the host C12N15/1082Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors C12N15/1086Preparation or screening of expression libraries C12N15/1089Design, preparation, screening or analysis of libraries using computer algorithms C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups C12N15/1096cDNA Synthesis; Subtracted cDNA library construction C12N15/11DNA or RNA fragments Modified forms thereof Non-coding nucleic acids having a biological activity C12N15/111General methods applicable to biologically active non-coding nucleic acids C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g.antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids C12N15/1131against viruses C12N15/1132against retroviridae C12N15/1133against herpetoviridae C12N15/1135against oncogenes or tumor suppressor genes C12N15/1136against growth factors, growth regulators, cytokines, lymphokines or hormones C12N15/1137against enzymes C12N15/1138against receptors or cell surface proteins C12N15/115Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith; Nucleic acids binding to non-nucleic acids C12N15/117Nucleic acids having immunomodulatory properties C12N15/52Genes encoding for enzymes or proenzymes C12N15/62DNA sequences coding for fusion proteins C12N15/625containing a sequence coding for a signal sequence C12N15/63Introduction of foreign genetic material using vectors Vectors Use of hosts therefor Regulation of expression C12N15/635Externally inducible repressor mediated regulation of gene expression C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host C12N15/65using markers C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation Use of non-functional linkers or adaptors C12N15/67General methods for enhancing the expression C12N15/68Stabilisation of the vector C12N15/69Increasing the copy number of the vector C12N15/70Vectors or expression systems specially adapted for E. coli C12N15/71Expression systems using regulatory sequences derived from the trp-operon C12N15/72Expression systems using regulatory sequences derived from the lac-operon C12N15/73Expression systems using phage (lambda) regulatory sequences C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli C12N15/743for Agrobacterium; Rhizobium; Bradyrhizobium C12N15/746for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus) C12N15/75for Bacillus C12N15/76for Actinomyces for Streptomyces C12N15/77for Corynebacterium for Brevibacterium C12N15/78for Pseudomonas C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts C12N15/80for fungi C12N15/81for yeasts C12N15/815for yeasts other than Saccharomyces C12N15/82for plant cells C12N15/8201Methods for introducing genetic material into plant cells C12N15/8202by biological means C12N15/8203Virus mediated transformation C12N15/8205Agrobacterium mediated transformation C12N15/8206by physical or chemical, i.e. non-biological, means C12N15/8207by mechanical means C12N15/8209Selection, visualisation of transformants, reporter constructs C12N15/821Non-antibiotic resistance markers C12N15/8212Colour markers C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination C12N15/8214Plastid transformation C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells C12N15/8217Gene switch C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] C12N15/822Reducing position variability C12N15/8221Transit peptides C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation C12N15/8223Vegetative tissue-specific promoters C12N15/8225Leaf-specific C12N15/8226Stem-specific C12N15/8227Root-specific C12N15/8229Meristem-specific C12N15/823Reproductive tissue-specific promoters C12N15/8231Male-specific C12N15/8233Female-specific C12N15/8234Seed-specific C12N15/8235Fruit-specific C12N15/8237Externally regulated expression systems C12N15/8238chemically inducible C12N15/8239pathogen inducible C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology C12N15/8242with non-agronomic quality (output) traits C12N15/8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering C12N15/8245involving modified carbohydrate or sugar alcohol metabolism C12N15/8246Non-starch polysaccharides C12N15/8247involving modified lipid metabolism C12N15/8249involving ethylene biosynthesis, senescence or fruit development C12N15/825involving pigment biosynthesis C12N15/8251Amino acid content C12N15/8253Methionine or cysteine C12N15/8254Tryptophan or lysine C12N15/8255involving lignin biosynthesis C12N15/8257for the production of primary gene products C12N15/8258for the production of oral vaccines (antigens) or immunoglobulins C12N15/8259Phytoremediation C12N15/8261with agronomic (input) traits C12N15/8262involving plant development C12N15/8263Ablation; Apoptosis C12N15/8265Transgene containment C12N15/8266Abscission; Dehiscence; Senescence C12N15/8267Seed dormancy, germination or sprouting C12N15/8269Photosynthesis C12N15/827Flower development or morphology C12N15/8271for stress resistance C12N15/8273for drought, cold, salt resistance C12N15/8274for herbicide resistance C12N15/8275Glyphosate C12N15/8277Phosphinotricin C12N15/8278Sulfonylurea C12N15/8279for biotic stress resistance, pathogen resistance, disease resistance C12N15/8281for bacterial resistance C12N15/8282for fungal resistance C12N15/8283for virus resistance C12N15/8285for nematode resistance C12N15/8286for insect resistance C12N15/8287for fertility modification C12N15/8289Male sterility C12N15/829Female sterility C12N15/8291Hormone-influenced development C12N15/8293Abscisic acid [ABA] C12N15/8294Auxins C12N15/8295Cytokinins C12N15/8297Gibberellins; GA3 C12N15/8298Brassinosteroids C12N15/85for animal cells C12N15/8509for producing genetically modified animals C12N15/86Viral vectors C12N15/861Adenoviral vectors C12N15/8613Chimaeric vector systems comprising heterologous sequences for production of another viral vector C12N15/8616Special methods for targeting systems C12N15/863Poxviral vectors C12N15/8633Avian poxviral vectors C12N15/8636Vaccina virus vectors C12N15/864Parvoviral vectors C12N15/8645Adeno-associated virus C12N15/866Baculoviral vectors C12N15/867Retroviral vectors C12N15/8673Special methods for packaging systems C12N15/8676Special methods for targeting systems C12N15/869Herpesviral vectors C12N15/8695Herpes simplex virus-based vectors C12N15/87Introduction of foreign genetic material using processes not otherwise provided for C12N15/873Techniques for producing new embryos C12N15/877Techniques for producing new mammalian cloned embroys C12N15/8771Bovine embryos C12N15/8772Caprine embryos C12N15/8773Ovine embryos C12N15/8775Murine embryos C12N15/8776Primate embryos C12N15/8777Rabbit embryos C12N15/8778Swine embryos C12N15/88using micro-encapsulation C12N15/89using micro-injection C12N15/895using biolistic methods C12N15/90Stable introduction of foreign DNA into chromosome C12N15/902using homologous recombination C12N15/905in geasts C12N15/907in mammalian cells