Patent Application
20120066778
The invention relates to vectors and mammalian cells in a system useful for switching on or switching off gene expression in response to uric acid.
Uric acid homeostasis is essential for humans since this metabolite is an important radical scavenger and oxidative stress protectant reducing the development of cancer, Parkinson's disease and multiple sclerosis. However, due to the loss of urate oxidase during evolution, humans have a predisposition for hyperuricemia which favors the formation of urate crystal deposits associated with nephropathy, tumor lysis syndrome and gout.
Gout is the most common inflammatory arthritis affecting over 1% of the human population in industrialized countries and its prevalence is globally on the rise as the growing consumption of carbohydrates promotes obesity and hyperinsulinism, which reduces renal clearance of uric acid, the end product of purine metabolism. Because of the evolutionary loss of the urate oxidase, converting uric acid into the more soluble allantoin, blood uric acid levels are particularly high in humans, which improves oxidative stress resistance and might have been essential for hominid brain evolution by having prevented oxidative damage to the increasingly complex neuronal networks. High physiologic urate levels are typically well tolerated by humans since a urate transporter in the kidney regulates urate levels in the bloodstream. However, imbalances of urate homeostasis may lead to hyperuricemia and formation of pathologic monosodium urate crystal deposits in the joints, kidney and subcutaneous tissues which trigger urate-associated pathologies such as hypertension, cardiovascular disease, urate nephrolithiasis and gout.
Deinococcus radiodurans R1, which is among earth's most radiation-resistant organisms, has also evolved a remarkable ability to withstand other sources of DNA damage including ultraviolet radiation and oxidative stress (Daly, M. J., Nat. Rev. Microbiol. 7, 237-245 (2009)). Recently, a hypothetical uricase regulator (HucR) has been identified in D. radiodurans which was suggested to play a critical role in the cellular response to oxidative stress. Akin to mammals, D. radiodurans seems to take advantage of the radical scavenging activity of uric acid, yet needs to control its level to prevent crystallization. HucR was shown to bind to a dyad-symmetrical operator site (hucO) in the intergenic region of divergently transcribed hucR and a putative uricase suggesting that both genes are by default co-repressed by HucR, unless excessive uric acid levels trigger the release of HucR from hucO and induce uricase expression (Wilkinson, S. P. & Grove, A., J. Biol. Chem. 279, 51442-51450 (2004); J. Mol. Biol. 350, 617-630 (2005)).
The invention relates to a mammalian cell useful in detecting and/or degrading a harmful excess of uric acid comprising
(a) a vector comprising a genetic code for a bacterial uric acid sensor-regulator fused to a transactivation domain or a transrepressor domain; and
(b) a vector comprising an operator sequence specifically binding said bacterial uric acid sensor-regulator, a promoter and a polynucleotide coding for an endogenous or exogenous protein, e.g. a protein interacting with uric acid.
Likewise, the invention relates to a vector comprising a genetic code for a bacterial uric acid sensor-regulator fused to a transactivation domain or a transrepressor domain as such, and to a vector comprising an operator sequence specifically binding said bacterial uric acid sensor-regulator, a promoter and a polynucleotide coding for an endogenous or exogenous protein as such.
In particular the invention relates to such a mammalian cell and to such vectors wherein the bacterial uric acid sensor-regulator is uricase sensor-regulator HucR from Deinococcus radiodurans R1, and the operator sequence is the corresponding operator sequence hucO from Deinococcus radiodurans R1.
Furthermore the invention relates to the mentioned mammalian cell in a nano- or microcontainer, a mammal excluding man comprising such a mammalian cell optionally in a nano- or microcontainer, and to a method of treating a disease caused by excess or lack of uric acid using such a mammalian cell.
(a) Expression vectors for uric acid-responsive transgene expression (UREX) (see Table 1 for abbreviations).
(b) Diagram of UREX in action. In the absence of uric acid (−UA) mUTS (KRAB-HucR) binds to hucO8 and represses SEAP production. In the presence of uric acid (+UA) mUTS is released from hucO8 which derepresses PUREX8 and results in PSV40-driven SEAP expression.
(c) mUTS-mediated repression of PUREX8 in different human cell lines. HeLa, HEK-293 and HT-1080 were co-transfected with pCK9 (PUREX8-SEAP-pA) and pCK25 (PhEF1α-mUTS-pA) and cultivated for 48 h prior to quantification of SEAP expression.
(d) Functional validation of the UREX sensor circuit. 4×104 HeLa cells were co-transfected with the UREX expression vectors pCK9 and pCK25 (see
(e-g) Urate-dependent dose-response characteristics of UREX in the presence and absence of the human urate transporter URAT1. 3×105 HeLa (e), HEK-293 (f) or HT-1080 cells (g) were co-transfected with the UREX sensor plasmids pCK9 and pCK25 (see
(S) SEAP production; (U) uric acid concentration; (mUTS) mammalian urate transsilencer.
(a) HeLaURAT1 cells engineered for UREX-controlled SEAP expression were microencapsulated in coherent alginate-poly-L-lysine-alginate microcapsules and intraperitoneally injected (2×106 cells per mouse, 200 cells/capsule) into (i) untreated uox−/− mice exhibiting pathologic urate levels or (ii) into uox−/− mice which had received 150 μg/mL (w/v) of the hyperuricemia therapeutic allopurinol in their drinking water to reduce urate levels (UREX). Control implants contained cells transgenic for constitutive SEAP expression (Control). SEAP levels were profiled in the serum of the animals 72 h after cell implantation.
(b) Urate-based dose-response profile of the triple-transgenic HEK-293UREX15 cell line stably engineered for UREX-controlled SEAP and constitutive URAT1 expression. 5×104 HEK-293UREX15 were cultivated in the presence of different urate concentrations and SEAP levels were quantified in the culture supernatant after 72 h.
(c) Reversibility of the UREX-based uric acid sensor circuit. 2×105 HEK-293UREX15 cells were cultivated for 10 days while alternating uric acid concentrations from 0 to 5 mM every 72 h (arrows).
(C) control; (S) SEAP production; (U) uric acid concentration; (+A) +Allopurinol (low urate); (−A) −Allopurinol (high urate); (t) time.
(a) Profiling of urate reduction mediated by constitutive or UREX-controlled expression of an intracellular (mUox) or secretion-engineered (smUox) urate oxidase variant. 2×105 HeLaURAT1 cells were co-transfected with either (i) pCK65 (PUREX8-smUox-pA) and isogenic control vector pcDNA3.1, (ii) pCK65 (PUREX8-smUox-pA) and pCK25 (PhEF1α-mUTS-pA), (iii) pCK67 (PUREX8-mUox-pA) and pcDNA3.1 or (iv) pCK67 (PUREX8-mUox-pA) and pCK25 (PhEF1α-mUTS-pA). The cells were cultivated for 72 h with 0.5 mM uric acid before urate levels were determined in the culture supernatant.
(b) Urate degradation profiles UREX-controlled smUox expression. 2×105 HeLaURAT1 cells (solid line) engineered for constitutive (dotted line) or UREX-controlled smUox (dashed line) expression were cultivated in medium containing a starting uric acid concentration of 0.5 mM and uric acid reduction kinetics were profiled for 120 h.
(c,d) UREX-controlled smUox-mediated reduction of pathologic uric acid levels in mice. Microencapsulated HeLaURAT1 cells engineered for UREX-controlled smUox expression were intraperitoneally implanted (2×106 cells per mouse) into untreated uox4″ mice exhibiting pathologic urate levels or into uox−/− mice having received 150 μg/mL (w/v) of the hyperuricemia therapeutic allopurinol in their drinking water (UREX-smUox). Control implants contained parental HeLaURAT1 (Control). Uric acid levels were profiled in serum (c) and urine (collected for 24 h) (d) of the animals 72 h after cell implantation.
(e-g) Tissue sections showing anisotropic monosodium urate crystal deposits (arrows) in the kidneys of (e) uox−/− mice receiving 150 μg/mL (w/v) allopurinol (positive control), (f) uox−/− mice (negative control) and (g) uox−/− mice implanted with HeLaURAT1 engineered for UREX-controlled smUox expression.
(C) control; (U) uric acid concentration; (SU) serum uric acid; (UU) urinary uric acid; (+A) +Allopurinol (low urate); (−A) −Allopurinol (high urate); (t) time. Scale bars 100 μM.
The herein described “UREX” system is a biosensor for self-sufficient auto-control of uric acid homeostasis. The system can be applied in vitro and in vivo as a general biosensor for the detection and response to uric acid. The UREX system can be used to either (i) switch on or (ii) switch off gene expression in response to uric acid in mammalian cells.
Uric acid is 7,9-dihydro-3H-purine-2,6,8-trione of the formula
and exists in several tautomeric forms. Uric acid as used herein includes all tautomeric forms of uric acid and salts thereof, called “urates”. Salts are, for example, monosodium urate or mono- or dipotassium urate.
The invention relates to a mammalian cell useful in detecting and/or degrading a harmful excess of uric acid comprising
(a) a vector comprising a genetic code for a bacterial uric acid sensor-regulator fused to a transactivation domain or a transrepressor domain; and
(b) a vector comprising an operator sequence specifically binding said bacterial uric acid sensor-regulator, a promoter and a polynucleotide coding for an endogenous or exogenous protein, e.g. a protein interacting with uric acid.
Likewise, the invention relates to a vector comprising a genetic code for a bacterial uric acid sensor-regulator fused to a transactivation domain or a transrepressor domain as such, and to a vector comprising an operator sequence specifically binding said bacterial uric acid sensor-regulator, a promoter and a polynucleotide coding for an endogenous or exogenous protein as such.
A bacterial uric acid sensor-regulator is, for the purposes of the invention, derived from or related to natural uric acid sensor-regulators such as HucR proteins produced by Deinococcaceae or Pseudomonadaceae. A bacterial uric acid sensor-regulator is, for example, the transcriptional regulator HucR (DR—1159) from Deinococcus radiodurans R1, the MarR type transcriptional regulator Dgeo—2531 from Deinococcus geothermalis DSM 11300, the MarR-type transcriptional regulator from Pseudomonas mendocina ypm, the MarR-type transcriptional regulator Deide—3p00280 from Deinococcus deserti VCD115, and sensor-regulators derived from these mentioned naturally occurring sensor-regulators. By “derived from” a natural uric acid sensor-regulator such as HucR protein produced by Deinococcaceae or Pseudomonadaceae, is meant, in this context, that the amino acid sequence is identical to a naturally occurring sensor-regulator, or contains only conservative amino substitutions and remains at least 70%, preferably 80%, and more preferably 90% identical at the amino acid level. By “related to” a natural uric acid sensor-regulator such as HucR proteins produced by Deinococcaceae or Pseudomonadaceae is meant, for purposes of the invention, that the polynucleotide sequence that encodes the amino acid sequence hybridizes to a naturally occurring uric acid sensor-regulator under at least low stringency conditions, more preferably moderate stringency conditions, and most preferably high stringency conditions, and binds to a natural uric acid sensor-regulator recognition sequence. Conservative substitution is known in the art and described by Dayhof, M. D., 1978, Nat. Biomed. Res. Found., Washington, D.C., Vol. 5, Sup. 3, among others. Genetically encoded amino acids are generally divided into four groups: (1) acidic=aspartate, glutamate; (2) basic=lysine, arginine, histidine; (3) non-polar=alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and (4) uncharged polar=glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine. Phenylalanine, tryptophan and tyrosine are also jointly classified as aromatic amino acids. A substitution of one amino acid in a particular group with another amino acid in the same group is generally regarded as a conservative substitution. It is understood, for the purpose of this invention, that “derived from” and “related to” also includes compounds at the polynucleotide level comprising triplet codons coding for the same amino acid but being especially adapted to the intended host cell, e.g. mammalian cell. Such polynucleotides especially adapted to mammalian cells are particularly preferred.
A corresponding operator sequence specifically binding said bacterial uric acid sensor-regulator is hucO, the tandem repeat-containing operator site recognized by HucR. Other operator sequences considered are those operator sequences specifically binding the mentioned sensor-regulators produced by Deinococcaceae or Pseudomonadaceae, and operator sequences “derived from” and “related to” the mentioned operator sequences. Under “derived from” is understood, for the purpose of this invention, that one or more nucleotides, in particular one to twelve, such as one, two, three, four or five nucleotides, may be replaced by other nucleotides without diminishing the intended interaction with the sensor-regulator. An operator sequence “related to” according to the invention hybridizes to a naturally occurring operator-sequence under at least low stringency conditions, more preferably moderate stringency conditions, and most preferably high stringency conditions, and binds to a natural uric acid sensor-regulator. Particular operator sequences considered are tandem repeats of naturally occurring operator sequences or sequences derived therefrom comprising, for example, between 2 and 100, in particular between 5 and 20, repeats.
A transactivation domain is, for example, the vp16 transactivation domain of Herpes simplex virus, the p65 transactivation domain, the human e2f4 transactivation domain, and transactivation domains derived from or related to GAL4, CTF/NF1, AP2, ITF1, Oct1 and SpI, and also those listed in U.S. Pat. No. 6,287,813.
A transrepressor domain is, for example, the krab transrepression domain of human Kruppel-associated box-protein. Other transrepressor domains considered are domains derived from or related to, for example, the v-erbA oncogenes product, the thyroid hormone receptor, the Ssn6/Tup1 protein complex, the SIR1 protein, NeP1, TSF3, SF1, WT1, Oct-2.1, E4BP4, ZF5, any transcriptional regulator containing a PhD bromo domain and also those listed in U.S. Pat. No. 6,287,813.
A promoter considered is, for example, the constitutive simian virus 40 promoter (PSV40), the minimal human cytomegalovirus immediate early promoter (PhCMVmin), the constitutive human cytomegalovirus promoter (PhCMV), the human elongation factor 1α promoter (PhEF1α), the phosphoglycerate kinase promoter (PPGK), the human ubiquitin promoter (PhUBC) and the beta-actin promoter.
An endogenous or exogenous protein considered in the context of the vectors and mammalian cells of the invention is, e.g., a protein interacting with uric acid, such as metabolising uric acid, for example uricase (urate oxidase) converting uric acid and its salts into allantoin, or transporting uric acid and its salts, for example mammalian urate transporter protein, in particular human urate transporter protein 1 (URAT1), or a protein easily detectable allowing quantification of uric acid.
A protein easily detectable is, for example, human placental secreted alkaline phosphatase (SEAP), a fluorescent or enhanced fluorescent protein (e.g. GFP, RFP, YFP, and the like), secreted alpha amylase (SAMY), luciferase, beta-galactosidase, beta-lactamase, and glucoronidase.
In particular the invention relates to such a mammalian cell and to such vectors wherein the bacterial uric acid sensor-regulator is uricase sensor-regulator HucR from Deinococcus radiodurans R1, and the operator sequence is the corresponding operator sequence hucO from Deinococcus radiodurans R1.
If, for example, the protein interacting with uric acid is urate oxidase (“uricase”), such as the therapeutically applicable rasburicase from Aspergillus flavus marketed under the trade name Fasturec®, the corresponding particular mammalian cell as described hereinafter represents an auto-level gene control system responding to pathological uric acid levels switching off transgene expression at non-pathological and oxidative stress protecting uric acid levels in urate oxidase deficient mice (representing an approved disease model for human hyperuricemic diseases like gouty arthritis and tumor lysis syndrome).
Systemic uric acid is a strong radical scavenger and protects the body from oxidative stress by detoxification of radicals. Only at highly elevated levels urate crystallizes in the joints, the kidney and subcutaneous tissues and causes painful inflammations and diseases like hyperuricemia, kidney stones and gouty arthritis. In contrast, the complete removal of uric acid from the body by constitutive expression of urate oxidase may result in an increased radical-based cellular and tissue damage, especially of neuronal cells, which is also shown by the protective nature of uric acid for neurodegenerative diseases like Alzheimer and Parkinson's disease. Therefore the maintenance of sub-pathological metabolite levels is an important step forward to novel gene-therapeutic applications.
The particular synthetic mammalian uric acid homeostasis control network as described hereinafter results in a dose-dependent urate oxidase-based therapeutic response. In urate oxidase-deficient mice developing hyperuricemia with human-like symptoms, the designer circuit decreases blood uric acid concentration to stable sub-pathologic levels and reduces urate crystal deposits in the kidney of treated animals. This therapeutic network provides self-sufficient automatic control of uric acid homeostasis by preventing critical urate accumulation while preserving basal uric acid levels for oxidative stress protection.
The mammalian UREX system as described hereinafter may also be used in the production of protein therapeutics in bioreactor applications. The production of transgenic enzymes, like e.g. members of the enzyme class of oxidases/reductases, may result in the enzyme-based catalytic conversion of compounds in media used for maintenance and growth of cells, and cause the release of radicals. The induced oxidative stress may perturb the continuous product formation by inhibiting cell growth or reducing the product stability. When controlling the expression of such enzymes with the UREX system the product formation is induced by the addition of exogenous uric acid which functions as an inducer of the enzyme expression as well as protecting the cells from process-borne oxidative stress by binding and detoxifying the generated radicals.
The invention further relates to a mammalian cell comprising the mentioned vectors, either stably or transiently transfected with the described vectors, and to such mammalian cells in a nanocontainer or microcontainer, e.g. in encapsulated form. A nanocontainer may be a virus, preferably an attenuated virus, in particular a viral capsid, synthetic or semi-synthetic nano- or microparticles, such as spheres or tubes of a suitable geometry to incorporate mammalian cells, and the nano- or microcontainers formed in situ by encapsulation of mammalian cells, for example with alginate-poly-L-lysine. A particular example of a suitable nano- or microcontainer is the hollow fibre manufactured under the trade name CELLMAX®.
The invention further relates to a mammal excluding man comprising a mammalian cell as described, in particular a mammalian cell in a nano- or microcontainer.
The invention further relates to a method of treating a disease caused by excess of uric acid or lack of uric acid using such a mammalian cell. Diseases considered are those caused by excess of uric acid, e.g. hyperuricemia, gout, Lesch-Nyhan syndrome, nephropathy, and tumor lysis syndrome, and diseases caused by an insufficient level of uric acid, such as Alzheimer, Parkinson, and corresponding cancers. In particular, the invention relates to a method of treatment of a patient suffering from a disease caused by excess or lack of uric acid wherein a mammalian cell in a nano- or microcontainer as described herein is implanted into a patient in need thereof.
In a particular exemplary embodiment of the invention, a particular synthetic circuitry enabling mammalian cells to sense and respond to uric acid is constructed, capitalizing on the uric acid-responsive interaction between HucR and hucO coordinating oxidative stress response in Deinococcus radiodurans R1 (Wilkinson, S. P. & Grove, A., loc.cit.).
This preferred embodiment of the invention is as follows: A uric acid-responsive expression network (UREX) is constructed by a multistep engineering strategy involving (i) modification of the HucR start codon (GTG→ATG) and fusion to a Kozak consensus sequence for maximum expression in mammalian cells (mHucR), (ii) N-terminal fusion of mHucR to the Krueppel-associated box protein domain (Bellefroid, E. J., Poncelet, D. A., Leco{tilde over (c)}q, P. J., Revelant, O. & Martial, J. A., Proc. Natl. Acad. Sci. USA 88, 3608-3612 (1991)), producing a chimeric mammalian urate-dependent transsilencer (mUTS, KRAB-mHucR) and (iii) a synthetic promoter assembled by placing eight (PUREX8, PSV40-hucO8) tandem hucO modules downstream of a simian virus 40 promoter (PSV40) (
In particular the invention relates to a mammalian cell and to vectors wherein the bacterial uric acid sensor-regulator is uricase sensor-regulator HucR from Deinococcus radiodurans R1, and the operator sequence is the corresponding operator sequence hucO from Deinococcus radiodurans R1. Further preferred embodiments are those wherein the uricase sensor-regulator HucR is fused to a Kozak consensus sequence, and wherein the Krueppel-associated box protein domain is N-terminally fused to the uricase sensor-regulator HucR. Other preferred embodiments are those wherein the promoter is the simian virus 40 promoter, and wherein the vector comprising the preferred operator sequence hucO from Deinococcus radiodurans R1 comprises hucO in several copies, e.g. between 5 and 50 copies, preferably between 5 and 20 copies. Most preferred are the vectors pCK25 and pCK9, and vectors wherein the codons for SEAP in pCK9 are replaced by codons for another detectable protein, e.g. for those easily detectable proteins listed above.
Because of the evolutionary loss of hepatic uricase by mutational silencing, humans depend on the urate-anion transporter URAT1 for effective renal urate clearance and maintenance of uric acid homeostasis (Enomoto, A. et al., Nature 417, 447-452 (2002)). Owing to the transporter's urate-uptake capacity ectopic expression of URAT1 may increase intracellular uric acid levels and consequently amplify UREX sensitivity. HeLa, HEK-293 and HT-1080 engineered for UREX-controlled SEAP expression and transfected with a constitutive URAT1 expression vector (pURAT1) reach significantly higher SEAP levels compared to URAT1-free cells and are particularly more sensitive within the urate range typically found in the human blood (200-400 μM) (
In order to characterize adjustability and long-term reversibility of the mammalian uric acid sensor system the stable human cell line HEK-293UREX15 is constructed which is triple-transgenic for UREX-controlled SEAP and URAT1 expression. SEAP expression of HEK-293UREX15 can be precisely adjusted and shows a progressive increase in response to escalating concentrations of uric acid (
As a precise and robust uric acid sensor UREX is suitable for therapeutic control of pathologic levels of this metabolite. For feedback-controlled reduction of uric acid levels in an autonomous and self-sufficient manner the uric acid sensor circuit needs to be linked to expression of a uricase/urate oxidase (Uox) which converts urate to the more soluble and renally secretable allantoin (Legoux, R. et al., J. Biol. Chem. 267, 8565-8570 (1992)). The cofactor-independent Aspergillus flavus uricase is codon-optimized for expression in mammalian cells (mUox). mUox reduces uric acid (0.5 mM) in the culture medium when constitutively expressed (pCK67, PUREX8-mUox-pA) or controlled by mUTS (pCK67 with co-expressed pCK25, PUREX8-mUox-pA, PhEF1α-mUTS-pA). Urate reduction is more efficient when mUox is engineered for mammalian cell-based secretion by in-frame fusion to an immunoglobulin-derived secretion signal (Fluri, D. et al., J. Control. Release 131, 211-219 (2008), smUox; SSIgk-mUox; pCK65, PUREX8-SSIgk-mUox-pA) (
The dynamics of UREX-controlled uric acid metabolism is profiled during a 120 h cultivation of HeLaURAT1 engineered for constitutive mUTS and PUREX8-driven smUox expression (
When uox−/− mice, developing hyperuricemia with human-like urate nephropathy and gouty arthritis symptoms in the absence of allopurinol, are treated with UREX-controlled smUox expressing cell implants, their urate levels in the serum and the urine drops to concentrations reached by standard allopurinol therapy while control animals (HeLaURAT1 cell implants, no allopurinol) accumulate urate and develop acute gouty arthritic symptoms (
Gouty arthritis is the most painful rheumatic disease. Due to a urate oxidase deficiency urate blood levels of humans are up to 50 times higher than in other mammals which increases the risk to develop hyperuricemic diseases. The fact that uric acid is an essential free radical scavenger and oxidative stress protectant important to prevent development of cancer, Parkinson's disease and multiple sclerosis makes gouty arthritis treatment particularly challenging as urate levels need to be accurately adjusted to prevent formation of uric acids crystal deposits while providing sufficient oxidative stress protection. There are two main therapeutic strategies for the treatment of urate-borne diseases in clinical use: (i) administration of xanthine oxidase inhibitors like allopurinol which blocks purine metabolism and prevents endogenous uric acid production or (ii) intravenous injection of recombinant Aspergillus flavus uricase (e.g., Rasburicase®) which promotes clearance of urate from the bloodstream. While Rasburicase® is mainly used to prevent the tumor lysis syndrome in high-risk cancer patients, allopurinol, which is on the market for over forty years, has severe side effects, cannot be taken during an acute attack of gout and has to be discontinued when hyperuricemia-induced nephropathy reaches an advanced state. Febuxostat (Uloric®), another xanthine oxidase inhibitor, has recently been licensed by the US Food and Drug Administration (FDA) for the treatment chronic hyperuricemia.
By functional connection of the human urate transporter with an engineered D. radiodurans-derived mammalian uric acid sensor and a sensor-controlled as well as secretion-engineered A. flavus urate oxidase a synthetic circuit is designed which provides self-sufficient automatic control of uric acid levels in mice. The network's unique auto-level urate control dynamics enables (i) seamless monitoring of uric acid levels in the blood, (ii) automatic induction of the core sensor unit at pathologic uric acids levels, (iii) prompt reduction of urate by sensor-controlled expression of clinically licensed urate oxidase and (iv) spontaneous shut-down when reaching basal physiologic urate levels needed for oxidative stress protection. UREX-based control of uric acid levels in a mouse model of human gouty arthritis shows that the synthetic circuit operates as expected and most importantly within the clinically relevant concentration range. In humans, painful inflammations resulting from monosodium urate crystals form at blood urate levels of above 6.8 mg/dl (Terkeltaub, R., Bushinsky, D. A. & Becker, M. A., Arthritis Res. Ther. 8, S4 (2006)). The UREX sensor is able to capture such pathologic levels and trigger dose-dependent expression of secreted uricase which consequently reduces urate in the bloodstream of treated animals about 80% to 5 mg/dl. At a circulation level below 6 mg/dl urate crystal deposits are known to dissolve and patients are free of any clinical manifestation of gouty arthritis. Therefore, cell implants harboring a UREX-based network for self-sufficient auto-level control of uric acid levels in the bloodstream represent a prophylactic strategy or therapeutic intervention replacing periodic small-molecule administration in hyperuricemic diseases.
Design details of all plasmids and oligonucleotides are provided in Table 1.
Human cervical adenocarcinoma cells (HeLa, ATCC CCL-2), human embryonic kidney cells (HEK-293, ATCC CRL-1573) and human fibrosarcoma cells (HT-1080, ATCC CCL-121) are cultivated in Dulbecco's modified Eagle's medium (DMEM, Invitrogen, Base1, Switzerland, Cat. No. 52100-39) supplemented with 10% (v/v) fetal calf serum (FCS, PAN Biotech GmbH, Aidenbach, Germany, Cat. No. 3302, Lot No. P251110) and 1% (v/v) penicillin/streptomycin solution (P/S, PAN Biotech GmbH, Cat. No. P06-07100). All cell types are cultivated at 37° C. in a humidified atmosphere containing 5% CO2. HEK-293 are transfected using a standard CaPO4-based protocol (Weber, W. et al., Nucleic Acids Res. 31, e71 (2003)). HeLa were also transfected according to this standard CaPO4-based protocol, with the exception that the DNA precipitates are incubated with the cells for 12 hours before changing the medium. HT-1080 are transfected with FuGENE 6 (Roche Diagnostics AG, Basel, Switzerland, Cat. No. 11814443001) according to the supplier's procedure. Transfected cells are cultivated in DMEM supplemented with 10% (v/v) knockout serum replacement (KOSR, Invitrogen, Basel, Switzerland, Cat. No. 10828-028), 1% P/S and, optionally, with uric acid (Acros Organics, Geel, Belgium, Cat. No. 171290250) (standard medium). To establish uric acid-dependent dose-response characteristics, 3×105 cells are seeded per well of a six-well plate (Thermo Fisher Scientific, Roskilde, Denmark) and transfected as described above. The cells are trypsinized (200 μl Trypsin [PAN Biotech GmbH, Cat. No. P10-023500], 5 min., 37° C.), collected by centrifugation (2 min., 120×g) and resuspended in 1.5 ml standard medium. 100 μl of this cell suspension are transferred to individual wells of a 96-well plate, supplemented with different concentrations of uric acid and cultivated for 48 h before reporter gene expression is profiled in the culture supernatant.
HeLa-derived HeLaURAT1 cells, transgenic for the constitutive expression of the human renal urate-anion exchanger (URAT1), are constructed by co-transfection of pURAT1 (PhCMV-URAT1-pA; ImaGenes, Berlin, Germany, IMAGE ID: 5183650) and pZeoSV2 (conferring resistance to zeocin; [InvivoGEN, San Diego, USA, Cat. No. ant-zn-5]) at a ratio of 10:1 (3 μg total DNA). After a 14-day selection period using 200 μg/ml (w/v) zeocin cells are clonaly expanded and individual clones are profiled for URAT1 expression using qRT-PCR. The cell line HeLaURAT1 is chosen for further experiments. The triple-transgenic HEK-293-derived HEK-293UREX15 cell line, enabling urate-inducible SEAP expression, is constructed by sequential co-transfection and clonal selection of (i) pCK9 (PUREX8-SEAP-pA) and pCK25 (PhEF1α-KRAB-mHucR-pA, also carrying a constitutive expression cassette conferring resistance to blasticidin) (ratio of 1:1, 14-day selection in DMEM containing 10% FCS and 20 μg/ml (w/v) blasticidin [InvivoGen, San Diego, USA, Cat. No. ant-bl-1]) and (ii) pURAT1 (PhCMV-URAT1-pA) and pZeoSV2 (conferring resistance to Zeocin) (ratio of 10:1 [(3 μg total DNA], 14-day selection in DMEM containing 10% FCS, 20 μg/ml (w/v) blasticidin and 200 μg/ml (w/v) zeocin (InvivoGen, San Diego, Calif., USA, Cat. No. ant-zn-5). Individual HEK-293UREX clones are randomly picked and assessed for urate-triggered SEAP expression. HEK-293UREX15 is chosen for further studies.
SEAP levels are quantified in cell culture supernatants and mouse serum using a standard p-nitrophenylphosphate-based light absorbance time course (Berger, J., Hauber, J., Hauber, R., Geiger, R. & Cullen, B. R., Gene 66, 1-10 (1988)). Uric acid levels are assessed in cell culture supernatants, murine serum or urine using the Amplex® Red uric acid/uricase assay kit (Invitrogen, Basel, Switzerland, Cat. No. A22181) according to the manufacturer's protocol. Uric acid-containing samples are diluted in reaction buffer. The addition of uricase triggers the enzymatic conversion of uric acid to allantoin, CO2 and H2O2 that reacts, in the presence of horseradish peroxidase stoichiometrically with Amplex Red reagent to generate the red-fluorescent oxidation product resorufin which can be quantified at 585 nm.
Urate oxidase-deficient mice (uox−/−) developing hyperuricemia with human-like gouty arthritis symptoms (Wu, X. et al., loc.cit.) are used to validate the synthetic UREX-based uric acid control network in vivo (Charles River Laboratories, France, mouse strain: JAKS-2223). Since uox−/− mice die within 4 weeks of age breeding and long-term maintenance requires addition of 150 μg/mL (w/v) allopurinol (Sigma, Cat. No. A8003) to the drinking water. Two weeks before implantation of UREX-transgenic cells the allopurinol treatment of urate oxidase-deficient mice is either continued or stopped to produce animal groups with low or high pathogenic uric acid levels in the bloodstream, respectively. HeLaURAT1, transgenic for constitutive URAT1 expression are transiently co-transfected with pCK25 (PhEF1α-mUTS-pA) and either pCK9 (PUREX8-SEAP-pA), pSEAP2-control (PSV40-SEAP-pA) or pCK65 (PUREX8-smUox-pA) and then micro-encapsulated in 200 μm alginate-(poly-L-lysine)-alginate capsules (200 cells/capsule) using an Inotech Encapsulator Research IE-50R (EncapBioSystems Inc., Greifensee, Switzerland) according to the manufacturer's protocol and applying the following settings: 200 μm single nozzle, stirrer speed control set to 5 units, 20 ml syringe with a flow rate of 410 units, nozzle vibration frequency 1024 Hz, voltage for capsule dispersion 900 V. 700 μl PBS containing 2×106 encapsulated cells (104 capsules/mouse) are intraperitoneally injected into urate oxidase-deficient mice. Control mice are implanted with micro-encapsulated parental HeLaURAT1. 48 h post-implantation the mice are transferred to a clean cage and the urine of each treatment group is sampled for 24 h. 72 h after implantation the mice are sacrificed, their blood is collected and the serum isolated in microtainer SST tubes (Beckton Dickinson, Plymouth, UK) according to the manufacturer's protocol. All experiments involving animals are performed according to the directive of the European Community Council (86/609/EEC).
For microscopic analysis of uric acid deposits in the kidney of uox−/− mice implanted with microencapsulated cells transgenic for constitutive URAT1 and UREX-controlled smUox expression treated animals are split into treatment groups receiving 150 μg/mL (w/v) or no allopurinol in their drinking water as described above. Seven days after implantation, the mice are sacrificed, their kidneys explanted and fixed in 4% (w/v) paraformaldehyde (Sigma, Cat. No. P6148) in PBS for 4 h. The kidneys are washed in 10% (w/v) sucrose (Sigma, Cat. No. S1888) in PBS for 90 min, trimmed, dehydrated and embedded in paraffin. 3 μm sections of the tissues produced using an Ultracut device (Zeiss, Feldbach, Switzerland) are transferred to gelatinized microslides and air-dried overnight at 37° C. After paraffin removal by submersion in xylene (3×10 min), the tissues are rehydrated by sequential incubation (10 min) in decreasing ethanol concentrations (90%, 80%, 70%), rinsed twice in TBS (Tris-buffered saline; 50 mM Tris/HCl [pH 7.4], 100 mM sodium chloride) and stained with haematoxylin/eosin solution. The samples are visualized under polarized light to assess anisotropism using a Leica DMRBE microscope.
| Number | Date | Country | Kind |
|---|---|---|---|
| 09006711.7 | May 2009 | EP | regional |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP2010/002783 | 5/6/2010 | WO | 00 | 11/15/2011 |
