Claims
- 1. A plant system for producing a heterologous protein under defined, controlled environmental conditions, the plant system comprising a plant (a) transformed with an expression vector comprising a gene coding for the heterologous protein operably linked to a promoter that is selected for optimal expression under the defined environmental conditions of CEA; (b) that produces a large amount of plant biomass under the defined environmental conditions, and (c) that produces tissue and tissue extract wherein the heterologous protein is stable.
- 2. The plant system of claim 1 wherein the plant is selected from the group consisting of Solanum, Spinacia and Brassica.
- 3. The plant system of claim 1, wherein the plant is Solanum, the promoter is light-inducible and the defined environmental conditions of CEA include at least 12 hours of light per day.
- 4. The plant system of claim 1, wherein the promoter is from the ribulose bis-phosphate carboxylase (Rubisco) small subunit gene.
- 5. The plant system of claim 1, wherein the promoter is CO2-inducible and the defined environmental conditions include between about 350 and 2,500 ppm CO2.
- 6. The plant system of claim 1, wherein the promoter is heat-inducible and the defined environmental conditions include a temperature between about 28 and 40° C.
- 7. The plant system of claim 6, wherein the heat-inducible promoter is the promoter from the hsp80 gene.
- 8. The plant system of claim 1, wherein the promoter is a chemically inducible promoter.
- 9. The plant system of claim 8, wherein the promoter is from the pathogenesis-related beta 1,3 glucanase gene, lipoxygenase 1 gene or potato proteinase inhibitor I gene.
- 10. The plant system of claim 1, wherein the promoter is a dark-inducible promoter.
- 11. The plant system of claim 10, wherein the promoter is from the potato proteinase inhibitor I or aminotransferase gene.
- 12. The plant system of claim 1, wherein the promoter is a constitutive promoter.
- 13. The plant system of claim 12, wherein the promoter is from the tobacco rpL34 gene, the agrobacterium nopaline synthase gene or the CaMV 35S gene.
- 14. The plant system of claim 1, wherein the plant is potato which produces between about 0.2 and 5 kilogram fresh weight vines per plant.
- 15. The plant system of claim 1, wherein the plant is mustard which produces between about 0.2 and 250 grams dry weight greens per plant.
- 16. A method of producing heterologous protein in a transformed plant comprising the steps of:
a. transforming a plant with an expression vector comprising a gene coding for the heterologous protein operably linked to a promoter that is selected for optimal expression under defined environmental conditions of CEA; b. cultivating the plant under the defined environment conditions of CEA; and c. extracting the heterologous protein.
- 17. The method of claim 16, wherein the plant is selected from the group consisting of Solanum, Spinacia and Brassica.
- 18. The method of claim 16, wherein the plant is Solanum, the promoter is light-inducible and the defined environmental conditions include at least 12 hours of light per day.
- 19. The method of claim 18, wherein the promoter is from the Rubisco small subunit gene.
- 20. The method of claim 16, wherein the promoter is CO2-inducible and the defined environmental conditions include between about 350 and 2,500 ppm CO2.
- 21. The method of claim 16, wherein the promoter is heat-inducible and the defined environmental conditions include a temperature between about 28 and 40° C.
- 22. The method of claim 21, wherein the heat-inducible promoter is the promoter from the hsp80 gene.
- 23. The method in claim 16, wherein the promoter is chemically inducible.
- 24. The method in claim 23, wherein the chemically inducible promoter is from the pathogenesis-related beta 1,3 glucanase gene, lipoxygenase 1 gene or potato proteinase inhibitor I gene.
- 25. The method of claim 16, wherein the promoter is a dark-inducible promoter.
- 26. The method of claim 25, wherein the promoter is from the potato proteinase inhibitor I or aminotransferase gene.
- 27. The method of claim 16, wherein the promoter is a constitutive promoter.
- 28. The method of claim 27, wherein the promoter is from the tobacco rpL34 gene, the agrobacterium nopaline synthase gene or the CaMV 35S gene.
- 29. A method of making a plant system for production of a heterologous protein comprising the steps of:
a. identifying a plant that produces a large amount of plant biomass under controlled environmental conditions, that can be rapidly propagated vegetatively and produces tissues and soluble protein extracts that provide increased stability against proteolysis and other damage to heterologous protein targets; b. transforming the plant with an expression vector comprising a gene coding for the heterologous protein operably linked to a promoter that is selected for optimal expression under the defined environmental conditions of CEA; and c. selecting a transformed plant that (i) produces a large amount of the heterologous protein and (ii) the heterologous protein is stable in plant tissues and an extract made from the plant.
- 30. The method of claim 29, wherein the plant is potato and is selected to produce between about 0.2 and 5 kg fresh weight vines per plant.
- 31. The method of claim 29, wherein the plant is mustard and is selected to produce between about 0.2 and 250 grams dry weight greens per plant.
- 32. The method of claim 29, wherein the plant is potato and is selected to produce between about 10 and 1300 kg heterologous protein/acre/year.
- 33. The method of claim 29, wherein the plant is mustard and is selected to produce between about 8 and 1000 kg heterologous protein/acre/year.
- 34. The method of claim 29, wherein the plant is Solanum, the promoter is light-inducible and the defined environmental conditions include at least 12 hours of light per day.
- 35. The method of claim 34, wherein the promoter is from the ribulose bis-phosphate carboxylase (Rubisco) small subunit gene.
- 36. The method of claim 29, wherein the promoter is CO2-inducible and the defined environmental conditions include between 350 and 2,500 ppm CO2.
- 37. The method of claim 29, wherein the promoter is heat-inducible and the defined environmental conditions include a temperature between about 28 to 40° C.
- 38. The method of claim 37, wherein the heat-inducible promoter is the promoter from the hsp80 gene.
- 39. The method of claim 29, wherein the promoter is a chemically inducible promoter.
- 40. The method of claim 39, wherein the promoter is from the pathogenesis-related beta 1,3 glucanase gene, lipoxygenase 1 gene or potato proteinase inhibitor gene.
- 41. The method of claim 29, wherein the promoter is a dark-inducible promoter.
- 42. The method of claim 41, wherein the promoter is from the potato proteinase inhibitor I or aminotransferase gene.
- 43. The method of claim 29, wherein the promoter is a constitutive promoter.
- 44. The method of claim 43, wherein the promoter is from the tobacco rpL34 gene, the agrobacterium nopaline synthase gene or the CaMV 35S gene.
Parent Case Info
[0001] This is application claims priority to U.S. application Ser. No. 60/220,224 filed Jul. 24, 2000 that is herein incorporated by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60220224 |
Jul 2000 |
US |