Claims
- 1. An isolated cyclic lipopeptide acylase produced by Verticillium sp. No. 30085, which catalyzes deacylation of the acyl group R1 of a cyclic lipopeptide compound of the following general formula (I): whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl or a salt thereof, to give a cyclic peptide of the following general formula (II): wherein R2, R3, R4, R5, and R6 are the same groups as respectively defined above or a salt thereof; wherein the optimum pH for deacylation is from 3 to 5; the optimum temperature for deacylation is from 25 to 50° C.
- 2. The acylase according to claim 1 which catalyzes deacylation of the acyl group R1 of a cyclic lipopeptide compound of the following general formula: [I]: or a salt thereof, to give a cyclic peptide of the following general formula: or a salt thereof.
- 3. The isolated acylase of claim 1, whereinR5 is hydroxysulfonyl, and R6 is carbamoyl.
- 4. A process for producing a cyclic peptide compound or a salt thereof which comprises contacting a cyclic lipopeptide compound or salt thereof with the isolated acylase of claim 1 to deacylate the fatty acyl moiety of the substrate.
- 5. The isolated enzyme of claim 1, wherein said cyclic lipopeptide compound is selected from the group consisting of FR901379, Echinocandin B, and Aculeacin A.
- 6. The process of claim 4, wherein said cyclic lipopeptide has the following formula (I): whereinR1 is acyl; R2 is hydrogen or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl or a salt thereof.
- 7. The process of claim 4, wherein said cyclic peptide has the formula (II): whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl or a salt thereof.
- 8. The process of claim 4, wherein said cyclic lipopeptide compound is selected from the group consisting of FR901379, Echinocandin B, and Aculeacin A.
- 9. A process of producing a cyclic peptide compound or a salt thereof which comprises:culturing the strain Verticillium sp No. 30085 for a time and under conditions for the expression of an cyclic lipopeptide acylase; isolating said cyclic lipopeptide acylase as a crude or purified enzyme solution; contacting said cyclic lipopeptide acylase with a cyclic lipopeptide compound or salt thereof to deacylate the fatty acyl moiety of the substrate.
- 10. The process of claim 9, wherein said cyclic lipopeptide has the following formula (I): whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl or a salt thereof.
- 11. The process of claim 9, wherein said cyclic peptide has the formula (II): whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl or a salt thereof.
- 12. The process of claim 9, wherein said cyclic lipopeptide compound is selected from the group consisting of FR901379, Echinocandin B, and Aculeacin A.
- 13. The process of claim 9, wherein said acylase catalyzes deacylation of the acyl group R1 of a cyclic lipopeptide compound of the following general formula (I): whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl or a salt thereof, to give a cyclic peptide of the following general formula (II): wherein R2, R3, R4, R5, and R6 are the same groups as respectively defined above or a salt thereof; wherein the optimum pH for deacylation is from 3 to 5; the optimum temperature for deacylation is from 25 to 50° C.
- 14. A method of making the isolated acylase of clam 1, comprisingculturing the strain Verticillium sp No. 30085 for a time and under conditions for the expression of an cyclic lipopeptide acylase; isolating said cyclic lipopeptide acylase as a crude or purified enzyme solution.
Priority Claims (1)
Number |
Date |
Country |
Kind |
8/151948 |
Jun 1996 |
JP |
|
Parent Case Info
This application is a Division of application Ser. No. 09/147,352 filed on Dec. 31, 1998, now allowed as U.S. Pat. No. 6,146,872, which was originally filed as International Application Number PCT/JP97/02003 on Jun. 11, 1997.
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Entry |
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