Claims
- 1- An excretory-secretory product of Trichuris suis comprising at least one antibacterial activity which inhibits the growth of Campylobacter jejuni as an assay strain in a growth medium and wherein the antibacterial activity is resistant to boiling, freeze-thawing, trypsin, and pronase E.
- 2- The excretory-secretory product of the Trichuris suis of claim 1 wherein the antibacterial activity has a minimum inhibitory concentration and a minimum bactericide concentration of the bacterial activity which are about the same.
- 3- The excretory-secretory product of the Trichuris suis of claim 1 wherein the antibacterial activity which is not more than 10K MW.
- 4- The excretory-secretory product of the Trichuris suis of claim 1 wherein the antibacterial activity inhibits at least 50% of at least one species of cream positive or cream negative bacteria.
- 5- The excretory-secretory product of the Trichuris suis of claim 4 wherein the antibacterial activity inhibits a bacteria selected from the group consisting of Campylobacter jejuni, Campylobacter coli, Escherichia coli, and Staphylococcus aureus.
- 6- A method for producing an excretory-secretory product from Trichuris suis with at least one antibacterial activity which inhibits the growth of Campylobacter jejuni as an assay strain in a growth medium and which is resistant to boiling, freeze-thawing, and proteases which comprises:
(a) culturing the Trichuris suis in vitro in a serum-free medium; and (b) separating the excretory-secretory product with the antibacterial activity from the medium.
- 7- The method of claim 6 wherein the antibacterial activity has a minimum inhibitory concentration and a minimum bactericide concentration of the bacterial activity which are about the same.
- 8- The method of claim 6 wherein the antibacterial activity which is not more than 10K MW.
- 9- The method of claim 6 wherein the antibacterial activity inhibits at least 50% of at least one species of cream positive or cream negative bacteria.
- 10- The method of claim 9 wherein the antibacterial activity inhibits a bacteria selected from the group consisting of Campylobacter jejuni, Campylobacter coli, Escherichia coli, and Staphylococcus aureus.
- 11- A method for inhibiting a microorganism which comprises:
contacting the microorganism with an excretory-secretory product of Trichuris suis which comprises at least one antibacterial activity which inhibits the growth of Campylobacter jejuni as an assay strain in a growth medium and which is resistant to boiling, freeze-thawing, trypsin, and pronase E in an amount which inhibits the microorganism.
- 12- The method of claim 11 wherein the microorganism is a bacterium.
- 13- The method of claim 11 wherein the antibacterial activity has a minimum inhibitory concentration and a minimum bactericide concentration of the bacterial activity which are about the same.
- 14- The method of claim 11 wherein the antibacterial activity which is not more than 10K MW.
- 15- The method of claim 11 wherein the antibacterial activity inhibits at least 50% of at least one species of cream positive or cream negative bacteria.
- 16- The method of claim 15 wherein the antibacterial activity inhibits a bacteria selected from the group consisting of Campylobacter jejuni, Campylobacter coli, Escherichia coli, and Staphylococcus aureus.
- 17- A composition comprising at least one antibacterial activity isolated from excretory-secretory products of Trichuris suis which inhibits the growth of Campylobacter jejuni as an assay strain in a growth medium and wherein the antibacterial activity is resistant to boiling, freeze-thawing, trypsin, and pronase E in a carrier.
- 18- The composition of claim 17 wherein the antibacterial activity has a minimum inhibitory concentration and a minimum bactericide concentration of the bacterial activity which are about the same.
- 19- The composition of claim 17 wherein the antibacterial activity which is not more than 10K MW.
- 20- The composition of claim 17 wherein the antibacterial activity inhibits at least 50% of at least one species of Gram positive or Gram negative bacteria.
- 21- The composition of claim 20 wherein the antibacterial activity inhibits a bacteria selected from the group consisting of Campylobacter jejuni, Campylobacter coli, Escherichia coli, and Staphylococcus aureus.
- 22- The composition of claim 17 wherein the carrier is selected from the group consisting of filler, non-toxic buffer, physiological saline solution, water, alcohol, ointment, cream, gel, balm, paste, liquor, tincture, elixir, tablet, lotion, paste, capsule, spirit, and perenteral solution.
- 23- A method for isolating an isolated antibacterial activity from excretory-secretory products of Trichuris suis, wherein the antibacterial activity inhibits the growth of Campylobacter jejuni as an assay strain in a growth medium and wherein the antibacterial activity is resistant to boiling, freeze-thawing, trypsin, and pronase E, comprising:
(a) culturing the Trichuris suis in vitro in a serum-free medium to produce the excretory-secretory products; (b) removing the antibacterial activity from components of the secretory-excretory products which are greater than 10,000 MW; (c) separating the antibacterial activity removed from the secretory products which are greater than 10,000 MW by column chromatography; and (d) eluting the antibacterial activity from the column to produce the isolated antibacterial activity wherein the antibacterial activity inhibits the growth of Campylobacter jejuni as an assay strain in a growth medium and wherein the antibacterial activity is resistant to boiling, freeze-thawing, trypsin, and pronase E.
- 24- The method of claim 23 wherein the column chromatography is HPLC.
- 25- The method of claim 24 wherein the HPLC uses a reverse phase C18 column and a gradient to separate the antibacterial activity.
- 26- The method of claim 25 wherein the gradient is a 5-80% acetonitrile gradient in 0.1% trifluoroacetic acid.
- 27- The method of claim 26 wherein the antibacterial activity is eluted from the HPLC column at between about 1 to 10 minutes.
- 28- The method of claim 26 wherein the antibacterial activity is eluted from the HPLC column at between about 40 to 50 minutes.
- 29- The method of claim 26 wherein the antibacterial activity is eluted from the HPLC column at between about 60 to 70 minutes.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application claims priority to Provisional Application No. 60/246,203, which was filed Nov. 6, 2000.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
[0002] This invention was supported by National Institutes of Health Grant No. AI42348-03. The U.S. government has certain rights in this invention.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/US01/44070 |
11/6/2001 |
WO |
|