Detection of polymorphisms in the human 5-lipoxygenase gene

Abstract

The present invention is based at least in part on the discovery of polymorphisms within the 5-lipoxygenase gene which are included in a haplotype. Accordingly, the invention provides nucleic acid molecules having a nucleotide sequence of an allelic variant of a 5-LO gene. The invention also provides methods for identifying specific alleles of polymorphic regions of a 5-LO gene, methods for determining whether a patient has a more or less severe phenotype of an inflammatory disease or disorder such as asthma, methods for determining whether a patient will be more or less responsive to treatment with 5′LO inhibitors, forensic methods based on detection of polymorphisms within the 5-LO gene, and kits for performing such methods.

Description

BACKGROUND OF THE INVENTION

5-lipoxygenase (5-LO) is the first committed enzyme in the pathway leading to leukotriene synthesis and is responsible for the conversion of arachidonic acid to LTA4 via the unstable intermediate 5-hydroperoxy eicosotetraenoic acid (Samuelsson et al., (1987) Science 237:1171; Samuelsson (1983) Science 220:568). Leukotriene A4 can, in turn, be converted to leukotriene B4, C4, D4, or E4. Leukotrienes are potent local mediators which influence inflammatory and allergic response, including asthma, rheumatoid arthritis, psoriasis, thrombotic disease, ulcerative colitis, bronchitis, sinusitis, allergic and non-allergic rhinitis, and lupus. Leukotrienes (LTs) B4, C4, D4, and E4 have been shown experimentally to mimic the pathologic changes seen in asthma and to play a role in several inflammatory mechanisms that lead to airflow obstruction in asthma. Such mechanisms include bronchoconstriction, mucosal edema, increased secretion of mucus, and an inflammatory-cell infiltrate that is rich in eosinophils. (O'Bryne (1997) Chest 111:27S-34S). The slow-reacting substance of anaphylaxis is now known to be a mixture of leukotrienes C4, D4, and E4, all of which are potent bronchoconstrictors that exert their biological actions through specific ligand-receptor interactions thereby increasing vascular permeability and constricting smooth muscle (U.S. Pat. No. 5,750,565; Silverman, et al. (1998) Clin Exp Allergy 28 Suppl 5:164-70).

There have been research efforts to develop specific receptor antagonists or inhibitors of leukotriene biosynthesis, to prevent or minimize pathogenic inflammatory responses mediated by these compounds, including the inhibition of 5-LO. For example, European Patent Application Nos. 90117171.0 and 901170171.0 disclose indole, benzofuran, and benzothiophene lipoxygenase inhibiting compounds (U.S. Pat. No. 5,750,565). It has been established that treating patients with agents that have the capacity to inhibit 5-LO results in improvement in lung function, reduction in asthma symptoms, and decreased need for alternative asthma treatments (Persson et al., (1995) Anesthesiology 82:969; Israel et al., (1993) Ann. Int. Med. 119:1059). However, in patients with asthma, there is a heterogeneous response to treatment with 5-LO inhibitors. Mutations in the 5-LO gene sequence may be associated with responsiveness to therapy and/or susceptibility to diseases or disorders, e.g., 5 asthma.

The 5-LO gene has been cloned, both as a cDNA (Matsumoto et al., (1988) Proc. Natl. Acad. Sci. USA 85:3406; Dixon et al., (1988) Proc. Natl. Acad. Sci. USA 85:416; Balcarek et al., (1988) J. Biol. Chem. 263:13937) and as a genomic clone (Hoshiko et al., (1990) Proc. Natl. Acad. Sci. USA 87:9073; Funk et al., (1989) Proc. Natl. Acad. Sci. USA 86 : 2587 ). The 5-LO gene is approximately 85 kilobases in size and contains 14 exons and 15 introns. The region 88 to 212 base pairs upstream of the 5-LO translation start site contains a number of sequences known to be recognition sites for transcriptional regulators (Hoshiko et al., (1990) Proc. Natl. Acad. Sci. USA 87:9073, incorporated herein by reference). For example, binding sites for AP1 and Sp1, each of which can act as either a transcriptional activator or a transcriptional repressor, depending on context, are found in this region. With respect to the Sp1 binding site, it comprises 5 tandem Sp1 motifs (GGGCGG) found −147 to −176 base pairs upstream of the ATG translation start site. Deletions of the Sp1 motifs reduce transcription from this 5′ upstream regulatory element (Hoshiko et al. (1990) Proc. Natl. Acad. Sci. USA 87:9073). Sp1 binding sites comprising such motifs are similarly found in the promoter region of a variety of other genes (Li et al., (1995) Gene 164:229; Wariishi et al., (1995) Biochem. Biophys. Res. Commun. 216:729; Tang et al., (1995) Biochem. Biophys. Res. Commun. 213:673; Khachigian et al., (1995) J. Bio. Chem. 270:27679).

Certain polymorphisms in the 5-LO gene have been shown to be correlated with patient responsiveness to 5-LO inhibitor therapy (U.S. Pat. No. 6,090,547 by Drazen, et al. (2000)). Drazen et al. disclosed that asthma patients having polymorphisms associated with reduced 5-LO gene expression are less responsive to 5-LO inhibitor therapy than patients having normal 5-LO gene expression. Specifically, Drazen et al. showed that 5-LO promoters comprising a variant Sp1 binding site having 3, 4 or 6 Sp1 motifs were less active than the wild-type 5-LO promoter comprising a Sp1 bind site having 5 Sp1 motifs, and that asthma patients homozygous or heterozygous for 5-LO alleles comprising S-LO promoters having such variant Sp1 binding sites were less responsive to S-LO inhibitor therapy than patient homozygous for the wild-type 5-LO allele. Drazen et al. also showed that the 3 Sp1 motif binding site is in linkage disequilibrium with a G to A polymorphism in exon 2 of the 5-LO gene, and that the 4 Sp1motif binding site is in linkage disequilibrium with a C to T polymorphism in exon 1 of the 5-LO gene.

It would also be desirable to identify additional polymorphisms within the 5-LO gene which are in linkage disequilibrium with a variant Sp1 binding site in the 5-LO promoter. It would further be desirable, to provide prognostic, diagnostic, pharmacogenomic and therapeutic methods utilizing the identified polymorphisms.

SUMMARY OF THE INVENTION

The present invention relates to polymorphisms in the 5-LO gene. The invention is based, in part, on the discovery of a novel 5-LO haplotype which comprises five sequence polymorphisms within the 5-LO gene that are in linkage disequilibrium with each other. The five 5-LO polymorphisms are set forth in Table 1. Three of these, 5loprr1, 5lo01a and 5lo04a, are newly identified polymorphisms. The other two, 5lonrra and 5lonrrb, have been described by In et al. in J. Clinical invest. 99:1130-1137 (1997). The present invention is based, also in part, on the discovery that the haplotype of the invention is in linkage disequilibrium with any one of three variant Sp1 binding sites in the 5-LO promoter region that were previously reported by Drazen et al. U.S. Pat. No. 6,090,547). Accordingly, any one of the five polymorphisms of the invention is a marker for the other four polymorphisms and a variant Sp1 binding site having 3, 4 or 6 Sp1 motifs in the 5-LO promoter region. Moreover, since Drazen et al. (id.) demonstrated that asthma patients homozygous or heterozygous for a 5-LO allele comprising any one of such variant Sp1 binding sites are less susceptible to effective treatment with 5-LO inhibitor therapy, the haplotype of the invention is also a marker of reduced responsiveness amongst inflammatory or allergy disease patients to 5-LO inhibitor therapy.

The present invention is based further, in part, on the discovery that the 5lo01a polymorphism of the invention is associated with an abnormally low eosinophil count. That is, individuals heterozygous or homozygous for the 5lo01a polymorphism have been found to have significantly lower eosinophil levels compared to individuals not having the polymorphism. Thus, the haplotype of the invention and each of its component polymorphisms are all markers of low eosinophil levels.

The invention provides a method for identifying an inflammatory or allergy disease patient who is less susceptible to effective treatment with a 5-LO inhibitor, comprising determining the presence or absence of a 5-LO allelic variant comprising a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of the sequence in a nucleic acid sample from an inflammatory or allergy disease patient, wherein the presence of the 5-LO allelic variant in the sample indicates that the patient is less susceptible to effective treatment with a 5-LO inhibitor. In one embodiment, the 5-LO allelic variant comprises the sequences of those set forth in SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of each of those sequences. In another embodiment, the 5-LO allelic variant comprises the sequences of those set forth in SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, or the complement of each of those sequences. In yet another embodiment, the 5-LO allelic variant comprises: (a) a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of each of those sequences; and (b) a variant Sp1 binding site having a 3, 4 or 6 Sp1 motif. In yet another embodiment, the 5-LO allelic variant comprises: (a) a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, or the complement of each of those sequences; and (b) a variant Sp1 binding site having a 3, 4 or 6 Sp1 motif.

The invention also provides a method for identifying a patient with an abnormally low eosinophil level, comprising determining the presence or absence of a 5-LO allelic variant comprising a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of the sequence in a nucleic acid sample from a patient, wherein the presence of the 5-LO allelic variant in the sample indicates that the patient has an abnormally low eosinophil level. In one embodiment, the 5-LO allelic variant comprises the sequences of those set forth in SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of each of those sequences. In another embodiment, the 5-LO allelic variant comprises the sequences of those set forth in SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, or the complement of each of those sequences. In yet another embodiment, the 5-LO allelic variant comprises: (a) a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of each of those sequences; and (b) a variant Sp1 binding site having a 3, 4 or 6 Sp1 motif In yet another embodiment, the 5-LO allelic variant comprises: (a) a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, or the complement of each of those sequences; and (b) a variant Sp1 binding site having a 3, 4 or 6 Sp1 motif. In a preferred embodiment, the patient is an inflammatory or allergy disease patient. In a more preferred embodiment, the patient is an asthma patient.

It is believed that inflammatory or allergy disease patients having abnormally low eosinophil levels have milder disease symptoms than patients having normal eosinophil levels. Accordingly, the instant invention further provides a method for determining the severity of disease symptoms in an inflammatory or allergy disease patient comprising determining the presence or absence of a 5-LO allelic variant comprising a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of the sequence in a nucleic acid sample from the patient, wherein the presence of the 5-LO allelic variant in the sample indicates that the patient has or will less severe disease symptoms than patients having wild-type 5-LO alleles. In one embodiment, the 5-LO allelic variant comprises the sequences of those set forth in SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of each of those sequences. In another embodiment, the 5-LO allelic variant comprises the sequences of those set forth in SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, or the complement of each of those sequences. In yet another embodiment, the 5-LO allelic variant comprises: (a) a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, or the complement of each of those sequences; and (b) a variant Sp1 binding site having a 3, 4 or 6 Sp1 motif. In yet another embodiment, the 5-LO allelic variant comprises: (a) a sequence selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, or the complement of each of those sequences; and (b) a variant Sp1 binding site having a 3, 4 or 6 Sp1 motif. In a preferred embodiment, the patient is an asthma patient.

The invention also provides isolated nucleic acids comprising the novel 5-LO polymorphisms and the haplotype of the invention. The nucleic acid molecules of the invention include specific 5-LO allelic variants which differ from the reference 5-LO sequences set forth in SEQ ID NO:1 (GI 187166), SEQ ID NO: 2 (GI 8247778), SEQ ID NO:3 (GI 4502056). The preferred nucleic acid molecules of the invention comprise newly identified 5-LO allelic variants or portions thereof having the any of the novel polymorphisms shown in Table 1 (i.e., those comprising the sequence of any of those set forth in SEQ ID NOs:4-6 or the complement thereof), polymorphisms in linkage disequilibrium with the polymorphisms shown in Table 1, and combinations thereof. The haplotype also includes known polymorphisms. These known polymorphisms include a guanine to adenine change in the 5′ upstream regulatory element at residue 84 of SEQ ID NO:1 (GI 187166) and a guanine to adenine change in the 5′ upstream regulatory element at residue 137 of SEQ ID NO:1 (GI 187166) (In, et al. (1997) J. Clinical Invest. 99:1130-1137). These known polymorphisms are also listed in Table 1 under “Known SNPs” and correspond to SEQ ID NOs.:7 and 8. These known polymorphisms may be used in combination with the newly identified polymorphisms of the present invention in the diagnostic, prognostic, pharmacogenomic and therapeutic methods described herein.

The haplotype of the invention is in linkage disequilibrium with known polymorphisms in the promoter region of the 5-LO gene. These polymorphisms are variations at the Sp1 binding site as described in U.S. Pat. No. 6,090,547 and In, et al. ((1997) J. Clin. Invest. 99(5):1130-1137). The wild-type 5-LO gene has a Sp1 binding site comprising five Sp1 motifs in tandem. Each of the Sp1 motifs consists of the nucleotide sequence “GGCGGG” (SEQ ID NO:61). The wild-type Sp1 site is located at nucleotide residues 1670-1699 in the reference sequence GI 187166 (SEQ ID NO:1). Polymorphisms contained within the Sp1 binding site repeat which are referred to herein as “variant Sp1 binding sites” have the deletion of one, the deletion of two, or the addition of one, Sp1 binding motif in the promoter region of the 5-LO gene resulting in three, four, or six Sp1 motifs.

The nucleic acid molecules of the invention can be double- or single-stranded. Accordingly, in one embodiment of the invention, a complement of the nucleotide sequence is provided wherein the polymorphism has been identified. For example, where there has been a single nucleotide change from a guanine to an adenine in a single strand, the complement of that strand will contain a change from cytidine to thymine at the corresponding nucleotide residue.

Nucleic acids of the invention can function as probes or primers, e.g., in methods for determining the allelic identity of a 5-LO polymorphic region in a nucleic acid of interest. The invention further provides vectors comprising the nucleic acid molecules of the present invention; host cells transfected with said vectors whether prokaryotic or eukaryotic; and transgenic non-human animals which contain a heterologous form of a functional or non-functional 5-LO allele described herein. Such a transgenic animal can serve as an animal model for studying the effect of specific 5-LO allelic variations, including mutations, as well as for use in drug screening and/or recombinant protein production.

The invention further provides methods for determining the molecular structure of at least a portion of a 5-LO gene. In a preferred embodiment, the method comprises contacting a sample nucleic acid comprising a 5-LO gene sequence with a probe or primer having a sequence which is complementary to a 5-LO gene sequence, carrying out a reaction that would amplify and/or detect differences in a region of interest within the 5-LO gene sequence, and comparing the result of each reaction with that of a reaction with a control (known) 5-LO gene (e.g., a 5-LO gene from a human not afflicted with an inflammatory condition e.g., asthma, or another disease associated with an aberrant 5-LO activity) so as to determine the molecular structure of the 5-LO gene sequence in the sample nucleic acid. The method of the invention can be used for example in determining the molecular structure of at least a portion of an exon, an intron, a 5′ upstream regulatory element, or the 3′ untranslated region. In a preferred embodiment, the method comprises determining the identity of at least one nucleotide. In another preferred embodiment, the method comprises determining the nucleotide at residue 1000 of the reference sequence GI 8247778, any one of residues 472-477 of the reference sequence GI 187166, and/or residue 559 of the reference sequence GI 187166, and combinations thereof. In another embodiment, the method comprises determining the nucleotide at residue 1000 of the reference sequence GI 8247778, any one of residues 472-477 of the reference sequence GI 187166, and/or residue 559 of the reference sequence GI 187166, the nucleotide at residue 84 of GI 187166, and/or the nucleotide at residue 137 of GI 187166. In a further embodiment, the method comprises determining the nucleotide at residue 1000 of the reference sequence GI 8247778, any one of residues 472-477 of the reference sequence GI 187166, and/or residue 559 of the reference sequence GI 187166, and the number of Sp1 motifs in the promoter region of the 5-LO gene.

In another preferred embodiment, the method comprises determining the nucleotide content of at least a portion of a 5-LO gene, such as by sequence analysis. In yet another embodiment, determining the molecular structure of at least a portion of a 5-LO gene is carried out by single-stranded conformation polymorphism (SSCP). In yet another embodiment, the method is an oligonucleotide ligation assay (OLA). Other methods within the scope of the invention for determining the molecular structure of at least a portion of a 5-LO gene include hybridization of allele-specific oligonucleotides, sequence specific amplification, primer specific extension, and denaturing high performance liquid chromatography (DHPLC). In at least some of the methods of the invention, the probe or primer is allele specific. Preferred probes or primers are single stranded nucleic acids, which optionally are labeled.

The invention further provides forensic methods based on detection of polymorphisms in the 5-LO gene.

The invention also provides probes and primers comprising oligonucleotides which hybridizes to at least 6 consecutive nucleotides of any of the sequences set forth in SEQ ID NOs: 4-6, or to the complement of any of such sequences, or naturally occurring mutants or variants thereof. In preferred embodiments, the probe/primer further includes a label attached thereto, which is capable of being detected.

In another embodiment, the invention provides a kit for amplifying and/or for determining the molecular structure of at least a portion of a 5-LO gene, comprising a probe or primer capable of hybridizing to a 5-LO gene and instructions for use. In one embodiment, the probe or primer is capable of hybridizing to a 5-LO intron. In another embodiment, the probe or primer is capable of hybridizing to a 5-LO allelic variant, preferrably a variant corresponding to 5 loprr1, 5lo01a or 5lo04a. In another preferred embodiment, the polymorphic region is located in the 5′ upstream regulatory element. In a preferred embodiment, determining the molecular structure of a region of a 5-LO gene comprises determining the identity of the allelic variant of the polymorphic region. Determining the molecular structure of at least a portion of a 5-LO gene can comprise determining the identity of at least one nucleotide or determining the nucleotide composition, e.g., the nucleotide sequence.

A kit of the invention can be used, e.g., for determining whether a patient will or will not be responsive to effective treatment of a disease associated with a specific 5-LO allelic variant, e.g., asthma, with a 5-LO inhibitor. In a preferred embodiment, the invention provides a kit for determining whether a patient will or will not be responsive to treatment of a inflammatory or allergic disease or condition associated with abnormal leukotriene synthesis, for example, asthma. The kit of the invention can also be used in selecting the appropriate drug to administer to a patient to treat such a disease or condition.

In another aspect, the invention provides a kit for determining whether a inflammatory or allergic disease patient has a more moderate or more severe disease phenotype associated with a specific 5-LO allelic variant of a polymorphic region. In one embodiment, the disease or disorder is characterized by an abnormal 5-LO activity, e.g., aberrant 5-LO expression. In another embodiment, the disease or disorder is characterized by an abnormal eosinophil levels.

Other features and advantages of the invention will be apparent from the following detailed description and claims.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 depicts the nucleotide sequence corresponding to reference sequence GI 87166 (SEQ ID NO:1).

FIG. 2 depicts the nucleotide sequence corresponding to reference sequence GI 8247778 (SEQ ID NO:2).

FIG. 3 depicts the nucleotide sequence corresponding to reference sequence GI 4502056 (SEQ ID NO:3).

FIG. 4 depicts the nucleotide sequence corresponding to reference sequence GI 9887688 (SEQ ID NO:63).

DETAILED DESCRIPTION OF THE INVENTION

The present invention is based, in part, on the identification of polymorphisms in the 5-LO gene which are associated with inflammatory or allergic disease phenotypes, including the asthma phenotype. The present invention is based, also in part, on the discovery of a haplotype comprising five polymorphisms within the promoter region of the 5-LO gene. As used herein, the term “haplotype” refers to a set of polymorphisms which are in linkage disequilibrium with each other. That is, the polymorphisms comprising the haplotype segregate together. The haplotype identified herein comprises novel polymorphisms and previously known polymorphisms as described herein and as set forth in Table 1.

The haplotype of the invention also has been found to be in linkage disequilibrium with any one of the three variant Sp1 binding sites disclosed in U.S. Pat. No. 6,090,047. Ethnic populations differ in the linked variant Sp1 binding site. In Caucasian and Chinese populations, the haplotype is in linkage disequilibrium with the variant Sp1 binding site containing four Sp1 motifs. Whereas in the African American population, the haplotype is in linkage disequilibrium with any one of the three variant Sp1 binding sites (i.e., variant Sp1 binding sites having 3, 4 or 6 Sp1 motifs).

It has been determined that the presence of a variant Sp1 binding site in the 5-LO promoter region reduced 5-LO gene expression (U.S. Pat. No. 6,090,547). Therefore, given that the haplotype of invention is linked to a variant Sp1 binding site, the haplotype and its constituent polymorphisms are all markers of reduced 5-LO gene expression. That is, detection of the haplotype or any of its constituent polymorphims can be used to predict whether or not a patient has decreased 5-LO gene expression as compared to patients homozygous for the wild-type 5-LO gene.

The present invention also relates to the discovery that asthmatic individual heterozygous or homozygous for a 5-LO allele comprising the 5lo01a polymorphism have significantly decreased eosinophil levels as compared to related family members having no such allele. While not intending to be bound by any specific theory, the association between the 5lo01a polymorphism and reduced eosinophil levels may be explained by recent studies that showed that some metabolites of 5-LO can induce production of eosinophils (Urasaki, et al. (2001) J. Leukocyle Biol. 69(1):105-112 and Powell et al., (2001) J. Allergy. Clin. Immunol. 107(2):272-278). Specifically, where an allele comprises the 5lo01a polymorphism, the allele also necessarily comprises the haplotype of the invention and a variant Sp1 binding site. 5-LO alleles comprising a variant Sp1 binding site have reduced levels of 5-LO expression which, in turn, result in reduced levels of 5-LO metabolites, thereby ultimately resulting in a reduction in the number of activated eosinophils. Thus, the presence of the haplotype or one or more polymorphisms comprising the haplotype can be used to predict whether a patient has a greater chance of having abnormally low eosinophil count. Eosinophil counts are used as an index of asthmatic disease, and reflect an asthma-related phenotype (see, for example, Jatakanon, A., et al. (2000) Am. J. Respir. Crit. Care Med 161(1):64; Kamfar, et al. (1999) J. Asthma 36(2):153; and Lonnkivist, K. et al. (2001) J. Allergy Clin. Immunol. 107(5):812). High eosinophil counts in asthma patients are associated with a more severe asthma phenotype as compared to the asthma phenotype in asthma patients having low eosinophil counts. Accordingly, determination of any polymorphism comprising the haplotype of the invention can be used to predict whether an asthma patient has or will have a moderate disease phenotype. Moreover, determination of any polymorphism comprising the haplotype can be used to differentiate among asthmatic populations to those having a moderate disease phenotype and those having a relatively more severe disease phenotype. A disease phenotype, e.g., the asthma phenotype, may be defined by established clinical parameters (see, for example, Moffitt et al. (1994) Am. Fam. Phys. 50:1039).

Pharmacogenetic studies have shown that the genetic background of individuals play a role in determining the response of an individual to a specific drug. Thus, determining the allelic variants of 5-LO polymorphic regions of an individual can be useful in predicting how an individual will respond to a specific drug, e.g., a drug for treating a disease or disorder associated with aberrant 5-LO activity and/or an inflammatory or allergic disease such as asthma As described above, specific 5-LO polymorphisms comprising the haplotype of the present invention result in increased or decreased production or expression of the 5-LO polypeptide. Accordingly, the action of a drug necessitating interaction with a 5-LO protein will be different in individuals carrying such a 5-LO allele. Furthermore, identification of polymorphisms in the 5-LO gene which indicate responsiveness to 5-LO inhibitor therapy are beneficial in the treatment of inflammatory diseases such as asthma because they can serve as markers by which the most appropriate treatment for inflammatory diseases or disorders can be identified.

Accordingly, preferred embodiments of the present invention include methods of identifying inflammatory or allergy disease patients who are likely to be more or less responsive to treatment with 5-LO inhibitors (e.g., a 5-LO inhibitor as described in U.S. Pat. Nos. 5,703,093, 5,750,565 or 6,025,384, the contents of which are incorporated herein by reference). For example, the presence of a variant Sp1 binding site indicates decreased expression of the 5-LO gene and decreased responsiveness to treatment with 5-LO inhibitors (see U.S. Pat. No. 6,090,547 and In, et al. (1997), supra). Accordingly, the presence of any one of the polymorphisms comprising the haplotype of the present invention indicates the presence of a variant Sp1 binding site and thus the associated decreased expression of the 5 -LO gene and decreased responsiveness to treatment with 5-LO inhibitors.

Therefore, the methods of the invention can be used to predict or determine whether a patient will be more or less responsive to treatment with a 5-LO inhibitor or other compounds or agents which interfere with the leukotriene metabolic pathway. These methods are applicable to all inflammatory and allergic diseases in which treatment with 5-LO inhibitors or related agents is appropriate, and in particular, to asthma.

There are multiple alleles of the 5-LO gene. The reference 5-LO gene sequence designated herein is presumed to be the wild-type 5-LO gene sequence and comprises nucleotide sequences that have been deposited in GENBANK™ and assigned the Accession Number GI 187166, GI 8247778, and GI 4502056 (corresponding to SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, respectively). The present invention relates to variant alleles of the 5-LO gene that differ from the reference 5-LO gene sequence by at least one of the polymorphisms identified in Table 1, and those in linkage equilibrium therewith. The present invention thus relates to nucleic acids comprising such variant 5-LO alleles.

The invention further relates to nucleic acids comprising portions of such variant 5-LO alleles that contain the haplotype of the invention or any of the novel 5-LO polymorphisms identified in Table 1 and are at least 5 nucleotides or basepairs in length. Portions can be, for example, 5-10, 5-15, 10-20, 2-25, 10-30, 10-50 or 10-100 nucleotides or basepairs long. For example, a portion of a variant allele which is 21 nucleotides or basepairs in length includes a 5-LO polymorphism (i.e., a nucleotide which differs from the corresponding nucleotide in the reference 5-LO sequence) and twenty additional nucleotides or basepairs which flank the polymorphism in the variant allele. These additional nucleotides and basepairs can be on one or both sides of the polymorphism. Polymorphisms of the invention are defined in Table 1 with respect to specific reference 5-LO sequences identified in Table 1 (GI 187166, GI 8247778, or GI 4502056). For example, the 5loprr1 polymorphism consists of an adenine substitution for the guanine at residue 1000 of the GI 187166 reference sequence (SEQ ID NO:1).

The 5-LO polymorphisms of the present invention have been identified in the human 5-LO gene by analyzing the DNA of human populations. In particular, DNA samples from 144 individuals were obtained and used for polymorphism discovery. These 144 DNA samples included samples from a population of forty-eight North American Caucasian individuals, forty-eight African American individuals, and forty-eight individuals from throughout the Anhui province in East Central China. The same frequency of 5-LO allelic variants was identified in all three of these groups. Furthermore, the forty-eight DNA samples from the Chinese individuals are from unrelated control patients, enrolled at random. Those patients were assessed for a number of traits related to asthma and allergies. Among the primary variables of interest were lung function, a response to “skin-prick” tests, physician's diagnosis of asthma, total serum IgE, and peripheral blood eosinophils. The peripheral blood eosinophil count was measured using the Coulter counter technique as described in Barnard D. F., et al. (1989) Clin Lab Haematol 11 (3):255-66, and is expressed in terms of eosinophils per microliter. The allelic variants of the present invention were identified by performing denaturing high performance liquid chromatography (DHPLC) analysis, the polymerase chain reaction (PCR), and/or single stranded conformation polymorphism (SSCP) analysis of genomic DNA from independent individuals as described in Example 1, using PCR primers complementary to intronic sequences surrounding each of the exons, 3′ UTR, and 5′ upstream regulatory element sequences of the 5-LO gene. The nucleotide sequence of these PCR primers (having SEQ ID NOs.:9-60) is shown in Table 3 (see the Examples).

The presence of three novel polymorphisms in the human 5-LO gene were identified in the populations studied. Two of the polymorphisms were characterized as single nucleotide polymorphisms (SNPs). The remaining polymorphism comprises deletions of two or more nucleotides from the reference 5-LO sequence. This variant is referred to herein as a “deletion variant.”

One polymorphism found in the population screened is a change from a guanine to an adenine in the 5′ upstream regulatory element of the 5-LO gene at residue 1000 of the reference sequence GI 187166 polymorphism ID No. 5loprr1). A second polymorphism is a deletion in the 5′ upstream regulatory element at residues 472-477 of the reference sequence GI 187166 (polymorphism ID No. 5lo01a). A third polymorphism is a change from a guanine to an adenine in the 5′ upstream regulatory element at residue 559 of the reference sequence GI 187166 (polymorphism ID No. 5lo04a). These polymorphisms are listed in Table 1 and correspond to SEQ ID NOs: 4-6.

Additional polymorphisms which are included in the haplotype of the present invention have also been identified in the 5-LO gene. These polymorphisms include a guanine to adenine change in the 5′ upstream regulatory element at residue 84 of the reference sequence GI 187166 (polymorphism ID No. 5lonrra) and a guanine to adenine change in the 5′ upstream regulatory element at residue 137 of the reference sequence GI 187166 (polymorphism ID No. 5lonrrb (In, et al. (1997) J. Clinical Invest 99:1130-1137). These known polymorphisms are also listed in Table 1 under “Known SNPs” and correspond to SEQ ID NOs.:7 and 8. These known polymorphisms may be used in combination with the polymorphisms of the present invention in the diagnostic, prognostic, pharmacogenomic and therapeutic methods described herein.

The haplotype of the present invention were found to be in linkage disequilibrium with polymorphisms that have been identified in the promoter region of the 5-LO gene. These polymorphisms are variations in the Sp1 binding site as described in U.S. Pat. No. 6,090,547 and In, et al. ((1997) J. Clin. Invest. 99(5): 1130-1137), the contents of which are incorporated herein by reference. The wild-type 5-LO gene has a Sp1 binding site that comprises five Sp1 motifs in tandem at nucleotide residues 1670-1699 in the reference sequence GI 187166 (SEQ ID NO;1). Each of the motifs has the nucleotide sequence “GGCGGG” (SEQ ID NO:61). Polymorphisms at the Sp1 binding site are referred to herein as “variant Sp1 binding sites” and have the deletion of one or two Sp1 motifs from, or the addition of one Sp1 motif to the wild-type Sp1 binding site, resulting variant Sp1 binding sites containing three, four, or six Sp1 motifs. These known Sp1 binding site polymorphisms may be used in combination with the polymorphisms and haplotype of the present invention in the diagnostic, prognostic, pharmacogenomic and therapeutic methods described herein.

Table 1 contains a “polymorphism ID No.” in column 2, which is used herein to identify each individual 5-LO polymorphism. In Table 1, the nucleotide sequence flanking each polymorphism is provided in column 3, wherein the polymorphic residue(s), having the wild-type or reference nucleotide, is indicated in lower-case letters. There are 10 nucleotides flanking the polymorphic nucleotide residue (i.e., 10 nucleotides 5′ of the polymorphism and 10 nucleotides 3′ of the polymorphism). Column 1 indicates the sequence listing identifier number (SEQ ID NO.) of the sequence shown in column 3 but with a variant nucleotide at the residue(s) shown in lower-case letter(s) or with a deletion of the sequences contained within the parenthesis. For example, SEQ ID NO:4 contains an adenine (“a”) at the location indicated by the lower-case letter “g” in the corresponding sequence in column 3. Therefore, SEQ ID NO:4 is identical to the corresponding sequence in column 3, except that the “g” (guanine) residue is replaced by an “a” (adenine residue). The deletion variant, polymorphism ID Nos. 5lo1a, lacks the deleted residues, which are identified by parenthesis in column 3. In Table 1, the location of some primers that may be used to detect each polymorphism is indicated in column 4. For example, primer location “2” indicates that the primers were designed to flank exon 2 and a portion of the surrounding intronic sequence. In column 5, the location of the polymorphism within the 5-LO gene is also provided, identifying the polymorphism as located in the 5′ upstream regulatory element, the 3′ untranslated region, or in an intron or an exon. Column 5 also identifies, for each SNP or deletion, the specific nucleotide change (i.e., “5′ upstream regulatory element G/A” refers to a single nucleotide polymorphism within the 5′ upstream regulatory element resulting in a change from a guanine in the reference sequence to an adenine in the variant allele).

Each polymorphism is identified based on a change in the nucleotide sequence from a “reference sequence.” As used herein, the reference sequence is the nucleotide sequence of SEQ ID NO:1 which corresponds to GI 187166 (see FIG. 1 ), the nucleotide sequence of SEQ ID NO:2 which corresponds to GI 247778 (see FIG. 2 ), or the nucleotide sequence of SEQ ID NO:3, which corresponds to GI 4502056 (see FIG. 3 ).

To identify the location of each polymorphism in Table 1, a specific nucleotide residue in a reference sequence is listed for each polymorphism, where nucleotide residue number 1 is the first (i.e., 5′) nucleotide in GI 187166 (corresponding to SEQ ID NO:1), the first nucleotide in GI 8247778 (corresponding to SEQ ID NO:2) or the first nucleotide in GI 450205 (corresponding to SEQ ID NO:3). Column 6 lists the reference sequence and polymorphic residue(s) for each polymorphism. For example, polymorphism ID No. 5loprr1 is a single nucleotide polymorphism (SNP) in the 5′ upstream regulatory element of the 5-LO gene which results in a change from a guanine to an adenine in the reference sequence GI 187166 (corresponding to SEQ ID NO:1). The SNP is located at residue number 1000 in the reference sequence (SEQ ID NO:1) starting from residue 1 at the 5′ end of the nucleotide sequence.

As can be seen in Table 1, one polymorphism found in the population is a change from a guanine to an adenine in the 5′ upstream regulatory element region of the 5-LO gene at residue 1000 of GI 18766 (polymorphism ID No. 5loprr1) (SEQ ID NO:4). A second polymorphism is a deletion in the 5′ upstream regulatory element at residues 472477 of GI 18766 (polymorphism ID No. 5lo01a) (SEQ ID NO:5). A third polymorphism is a change from a guanine to an adenine in the 5′ upstream regulatory element at residue 559 of GI 18766 (polymorphism ID No. 5lo04a) (SEQ ID NO:6). The nucleic acid molecules of the invention can be double- or single-stranded. Accordingly, the invention further provides for the nucleic acid strands comprising sequences complementary to the sequences listed in column 3 of Table 1.

The invention further provides allele-specific oligonucleotides that hybridize to a gene comprising a polymorphism of the invention. Such oligonucleotides will hybridize to one polymorphic form of the nucleic acid molecules described herein but not to the other polymorphic form(s) of the sequence. Thus such oligonucleotides can be used to determine the presence or absence of particular alleles of the polymorphic sequences described herein. These oligonucleotides can be probes or primers.

Not only does the present invention provide for polymorphisms in linkage disequilibrium with the polymorphisms of Table 1, it also provides methods for revealing the existence of yet other polymorphisms in the human 5-LO gene. For example, the polymorphism studies described herein can also be applied to populations in which other inflammatory diseases or allergic disease or disorders are prevalent.

Other aspects of the invention are described below or will be apparent to one of skill in the art in light of he present disclosure.

Definitions

For convenience, the meaning of certain terms and phrases employed in the specification, examples, and appended claims are provided below.

The term “inflammatory disease or allergic disease or disorder” as used herein refers to any disease or disorder characterized by an aberrant inflammatory response. An aberrant inflammatory response includes, for example, abnormal (e.g., an increased or decreased) production of leukotriene molecules. Examples of inflammatory or allergic diseases or disorders include, but are not limited to, asthma, bronchitis, sinusitis, ulcerative colitis, nephritis, amyloidosis, rheumatoid arthritis, sarcoidosis, scleroderma, lupus, non-allergic rhinitis, polymyositis, Reiter's syndrome, psoriasis, pelvic inflammatory disease, orbital inflammatory disease, thrombotic disease, and inappropriate allergic responses to environmental stimuli such as poison ivy, pollen, insect stings and certain foods, including atopic dermatitis and contact dermatitis.

The term “allele,” which is used interchangeably herein with “allelic variant” and “variant allele”, refers to alternative forms of a gene or portions thereof. Alleles occupy the same locus or position on homologous chromosomes. When a patient has two identical alleles of a gene, the patient is said to be homozygous for the gene or allele. When a patient has two different alleles of a gene, the patient is said to be heterozygous for the gene. Alleles of a specific gene, including 5-LO, can differ from each other in a single nucleotide, or several nucleotides, and can include substitutions, deletions, and insertions of nucleotides. An allele of a gene can also be a form of a gene containing one or more mutations.

The term “allelic variant of a 5-LO gene” or “5-LO allelic variant” refers to an alternative form of the 5-LO gene having one of several possible nucleotide sequences found in same position within the gene in the population. The predominate alleles in the population are referred to as “wild-type” alleles.

“Biological activity” or “bioactivity” or “activity” or “biological function”, which are used interchangeably, for the purposes herein when applied to 5-LO, means an effector or antigenic function that is directly or indirectly performed by a 5-LO polypeptide (whether in its native or denatured conformation), or by a fragment thereof Biological activities include modulation of the stereo-specific addition of molecular oxygen to arachidonic acid to form leukotriene A4, thereby modulating synthesis of leukotrienes and modulating inflammatory response, and other biological activities, whether presently known or inherent. A 5-LO bioactivity can be modulated by directly affecting a 5-LO protein effected by, for example, changing the level of effector or substrate level. Alternatively, a 5-LO bioactivity can be modulated by modulating the level of a 5-LO protein, such as by modulating expression of a 5-LO gene. Antigenic functions include possession of an epitope or antigenic site that is capable of cross-reacting with antibodies that bind a native or denatured 5-LO polypeptide or fragment thereof.

Biologically active 5-LO polypeptides include polypeptides having both an effector and antigenic function, or only one of such functions. 5-LO polypeptides include antagonist polypeptides and native 5-LO polypeptides, provided that such antagonists include an epitope of a native 5-LO polypeptide. An effector function of 5-LO polypeptide can be the ability to bind to a ligand, e.g., an arachidonic acid or modified form thereof

As used herein the term “bioactive fragment of a 5-LO protein” refers to a fragment of a fill-length 5-LO protein, wherein the fragment specifically mimics or antagonizes the activity of a wild-type 5-LO protein. The bioactive fragment preferably is a fragment capable of binding to a second molecule, such as a ligand.

The term “an aberrant activity” or “abnormal activity”, as applied to an activity of a protein such as 5-LO, refers to an activity which differs from the activity of the wild-type (i.e., normal) protein or which differs from the activity of the protein in a healthy subject, e.g., a subject not afflicted with a disease associated with a 5-LO allelic variant. An activity of a protein can be aberrant because it is stronger than the activity of its wild-type counterpart. Alternatively, an activity of a protein can be aberrant because it is weaker or absent relative to the activity of its wild-type counterpart. An aberrant activity can also be a change in reactivity. For example an aberrant protein can interact with a different protein or ligand relative to its wild-type counterpart. A cell can also have aberrant 5-LO activity due to overexpression or underexpression of the 5-LO gene. Aberrant 5-LO activity can result from a mutation in the gene, which results, e.g., in lower or higher binding affinity of a lipid to the 5-LO protein encoded by the mutated gene. Aberrant 5-LO activity can also result from a lower or higher level of 5-LO receptor on cells, which can result, e.g., from a mutation in the 5′ flanking region of the 5-LO gene or any other regulatory element of the 5-LO gene, such as a regulatory element located in an intron. Accordingly, aberrant 5-LO activity can result from an abnormal 5-LO 5′ upstream regulatory element activity.

The terms “abnormal 5-LO 5′ upstream regulatory element activity”, “aberrant 5-LO 5′ upstream regulatory element activity”, “abnormal 5-LO promoter activity”, “aberrant 5-LO promoter activity”, “abnormal 5-LO transcriptional activity” and “aberrant 5-LO transcriptional activity”, which are used interchangeably herein, refer to the transcriptional activity of a 5-LO 5′ upstream regulatory element which differs from the transcriptional activity of the corresponding 5′ upstream regulatory element in the wild-type 5-LO allele. Abnormal 5-LO activity can result from a higher or lower transcriptional activity as compared to transcriptional activity of a wild-type 5-LO allele. Aberrant 5-LO 5′ upstream regulatory element activity can result, for example, from the presence of a genetic lesion in a regulatory element, such as in a 5′ upstream regulatory element. An “aberrant 5-LO 5′ upstream regulatory element activity” is also intended to refer to the transcriptional activity of a 5-LO 5′ upstream regulatory element which is functional (capable of inducing transcription of a gene to which it is operably linked) in tissues or cells in which the normal or wild-type 5-LO 5′ upstream regulatory element is not functional or which is non functional in tissues or cells in which the normal or wild-type 5-LO 5′ upstream regulatory element is functional. Thus, a tissue distribution of 5-LO in a patient which differs from the tissue distribution of 5-LO in a normal (e.g., healthy) individual, can be the result of abnormal transcriptional activity from the 5-LO 5′ upstream regulatory element. Such abnormal transcriptional activity can result from e.g., one or more mutations in a regulatory element, such as in a 5′ upstream regulatory element thereof Abnormal transcriptional activity can also result from a mutation in a transcription factor involved in the control of 5-LO gene expression.

“Cells,” “host cells” or “recombinant host cells” are terms used interchangeably herein. It is understood that such terms refer not only to the particular patient cell but to the progeny or derivatives of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

As used herein, the term “gene” or “recombinant gene” refers to a nucleic acid molecule comprising an open reading frame and including at least one exon and (optionally) an intron sequence. The term “intron” refers to a DNA sequence present in a given gene which is spliced out during mRNA maturation.

“Homology” or “identity” or “similarity” refers to sequence similarity between two peptides or between two nucleic acid molecules. Homology can be determined by comparing a position in each sequence which may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same base or amino acid, then the molecules are homologous at that position. A degree of homology between sequences is a function of the number of matching or homologous positions shared by the sequences. An “unrelated” or “non-homologous” sequence shares less than 40% identity, though preferably less than 25% identity, with one of the sequences of the present invention.

To determine the percent identity of two amino acid sequences or of two nucleic acids, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of a first amino acid or nucleic acid sequence for optimal alignment with a second amino or nucleic acid sequence). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity=number of identical positions/total number of positions (e.g., overlapping positions)×100). In one embodiment the two sequences are the same length.

The determination of percent identity between two sequences can be accomplished using a mathematical algorithm. A preferred, non-limiting example of a mathematical algorithm utilized for the comparison of two sequences is the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA 87:2264-2268, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 90:5873-5877. Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul, et al. (1990) J. Mol. Biol. 215:403-410. BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to a nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score=50, wordlength=3 to obtain amino acid sequences homologous to a protein molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402. Alternatively, PSI-Blast can be used to perform an iterated search which detects distant relationships between molecules. When utilizing BLAST, Gapped BLAST, and PSI-Blast programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used. Another preferred, non-limiting example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Myers and Miller, (1988) CABIOS 4:11-17. Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Yet another useful algorithm for identifying regions of local sequence similarity and alignment is the FASTA algorithm as described in Pearson and Lipman (1988) Proc. Natl. Acad. Sci. USA 85:2444-2448. When using the FASTA algorithm for comparing nucleotide or amino acid sequences, a PAM120 weight residue table can, for example, be used with a k-tuple value of 2.

The term “a homolog of a nucleic acid” refers to a nucleic acid having a nucleotide sequence having a certain degree of homology with the nucleotide sequence of the nucleic acid or complement thereof. For example, a homolog of a double stranded nucleic acid having SEQ ID NO:N is intended to include nucleic acids having a nucleotide sequence which has a certain degree of homology with SEQ ID NO:N or with the complement thereof. Preferred homologs of nucleic acids are capable of hybridizing to the nucleic acid or complement thereof.

The term “hybridization probe” or “primer” as used herein is intended to include oligonucleotides which hybridize bind in a base-specific manner to a complementary strand of a target nucleic acid. Such probes include peptide nucleic acids, and described in Nielsen et al., (1991) Science 254:1497-1500. Probes and primers can be any length suitable for specific hybridization to the target nucleic acid sequence. The most appropriate length of the probe and primer may vary depending on the hybridization method in which it is being used; for example, particular lengths may be more appropriate for use in microfabricated arrays, while other lengths may be more suitable for use in classical hybridization methods. Such optimizations are known to the skilled artisan. Suitable probes and primers can range form about 5 nucleotides to about 30 nucleotides in length. For example, probes and primers can be 5, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 25, 26, 28 or 30 nucleotides in length. The probe or primer of the invention comprises a sequence that flanks and/or preferably overlaps, at least one polymorphic site occupied by any of the possible variant nucleotides. The nucleotide sequence of an overlapping probe or primer can correspond to the coding sequence of the allele or to the complement of the coding sequence of the allele.

The term “interact” as used herein is meant to include detectable interactions between molecules, such as can be detected using, for example, a binding or hybridization assay. The term interact is also meant to include “binding” interactions between molecules. Interactions may be, for example, protein-protein, protein-nucleic acid, protein-small molecule or small molecule-nucleic acid in nature.

The term “intronic sequence” or “intronic nucleotide sequence” refers to the nucleotide sequence of an intron or portion thereof.

The term “isolated” as used herein with respect to nucleic acids, such as DNA or RNA, refers to molecules separated from other DNAs or RNAs, respectively, that are present in the natural source of the macromolecule. The term isolated as used herein also refers to a nucleic acid or peptide that is substantially free of cellular material, viral material, or culture medium when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. Moreover, an “isolated nucleic acid” is meant to include nucleic acid fragments which are not naturally occurring as fragments and would not be found in the natural state. The term “isolated” is also used herein to refer to polypeptides which are isolated from other cellular proteins and is meant to encompass both purified and recombinant polypeptides.

The term “linkage” describes the tendency of genes, alleles, loci or genetic markers to be inherited together as a result of their location on the same chromosome. It can be measured by percent recombination between the two genes, alleles, loci, or genetic markers. The term “linkage disequilibrium” refers to a greater than random association between specific alleles at two marker loci within a particular population. In general, linkage disequilibrium decreases with an increase in physical distance. If linkage disequilibrium exists between two markers within one gene, then the genotypic information at one marker can be used to make probabilistic predictions about the genotype of the second marker.

The term “locus” refers to a specific position in a chromosome. For example, a locus of a 5-LO gene refers to the chromosomal position of the 5-LO gene.

The term “modulation” as used herein refers to both upregulation, (i.e., activation or stimulation), for example by agonizing; and downregulation (i.e. inhibition or suppression), for example by antagonizing of a bioactivity (e.g. expression of a gene).

The term “molecular structure” of a gene or a portion thereof refers to the structure as defined by the nucleotide content (including deletions, substitutions, additions of one or more nucleotides), the nucleotide sequence, the state of methylation, and/or any other modification of the gene or portion thereof.

The term “mutated gene” refers to an allelic form of a gene that differs from the predominant form in a population. A mutated gene is capable of altering the phenotype of a patient having the mutated gene relative to a patient having the predominant form of the gene. If a patient must be homozygous for this mutation to have an altered phenotype, the mutation is said to be recessive. If one copy of the mutated gene is sufficient to alter the phenotype of the patient, the mutation is said to be dominant. If a patient has one copy of the mutated gene and has a phenotype that is intermediate between that of a homozygous and that of a heterozygous (for that gene) patient, the mutation is said to be co-dominant.

As used herein, the term “nucleic acid” refers to polynucleotides such as deoxyribonucleic acid (DNA), and, where appropriate, ribonucleic acid (RNA). The term should also be understood to include, as equivalents, derivatives, variants and analogs of either RNA or DNA made from nucleotide analogs, and, as applicable to the embodiment being described, single (sense or antisense) and double-stranded polynucleotides. Deoxyribonucleotides include deoxyadenosine, deoxycytidine, deoxyguanosine, and deoxythymidine. For purposes of clarity, when referring herein to a nucleotide of a nucleic acid, which can be DNA or an RNA, the terms “adenosine”, “cytidine”, “guanosine”, and thymidine” are used. It is understood that if the nucleic acid is RNA, a nucleotide having a uracil base is uridine.

The term “nucleotide sequence complementary to the nucleotide sequence set forth in SEQ ID NO:N” refers to the nucleotide sequence of the complementary strand of a nucleic acid strand having SEQ ID NO:N. The term “complementary strand” is used herein interchangeably with the term “complement”. The complement of a nucleic acid strand can be the complement of a coding strand or the complement of a non-coding strand. When referring to double stranded nucleic acids, the complement of a nucleic acid having SEQ ID NO:N refers to the complementary strand of the strand having SEQ ID NO:N or to any nucleic acid having the nucleotide sequence of the complementary strand of SEQ ID NO:N. When referring to a single stranded nucleic acid having the nucleotide sequence SEQ ID NO:N, the complement of this nucleic acid is a nucleic acid having a nucleotide sequence which is complementary to that of SEQ ID NO:N. The nucleotide sequences and complementary sequences thereof are always given in the 5′ to 3′ direction. The term “complement” and “reverse complement” are used interchangeably herein.

A “non-human animal” of the invention can include mammals such as rodents, non-human primates, sheep, goats, horses, dogs, cows, chickens, amphibians, reptiles, etc. Preferred non-human animals are selected from the rodent family including rat and mouse, most preferably mouse, though transgenic amphibians, such as members of the Xenopus genus, and transgenic chickens can also provide important tools for understanding and identifying agents which can affect, for example, embryogenesis and tissue formation. The term “chimeric animal” is used herein to refer to animals in which an exogenous sequence is found, or in which an exogenous sequence is expressed in some but not all cells of the animal. The term “tissue-specific chimeric animal” indicates that an exogenous sequence is present and/or expressed or disrupted in some tissues, but not others.

The term “oligonucleotide” is intended to include and single- or double stranded DNA or RNA. Oligonucleotides can be naturally occurring or synthetic, but are typically prepared by synthetic means. Preferred oligonucleotides of the invention include segments of 5-LO gene sequence or their complements, which include and/or flank any one of the polymorphic sites shown in Table 1. The segments can be between 5 and 250 bases, and, in specific embodiments, are between 5-10, 5-20, 10-20, 10-50, 20-50 or 10-100 bases. For example, the segments can be 21 bases. The polymorphic site can occur within any position of the segment or a region next to the segment. The segments can be from any of the allelic forms of 5-LO gene sequence shown in Table 1.

The term “operably-linked” is intended to mean that the 5′ upstream regulatory element is associated with a nucleic acid in such a manner as to facilitate transcription of the nucleic acid from the 5′ upstream regulatory element.

The term “polymorphism” refers to the coexistence of more than one form of a gene or portion thereof. A portion of a gene of which there are at least two different forms, i.e., two different nucleotide sequences, is referred to as a “polymorphic region of a gene”. A polymorphic locus can be a single nucleotide, the identity of which differs in the other alleles. A polymorphic locus can also be more than one nucleotide long. The allelic form occurring most frequently in a selected population is often referred to as the reference and/or wild-type form. Other allelic forms are typically designated or alternative or variant alleles. Diploid organisms may be homozygous or heterozygous for allelic forms. A diallelic or biallelic polymorphism has two forms. A trialleleic polymorphism has three forms.

A “polymorphic gene” refers to a gene having at least one polymorphic region.

The term “primer” as used herein, refers to a single-stranded oligonucleotide which acts as a point of initiation of template-directed DNA synthesis under appropriate conditions (e.g., in the presence of four different nucleoside triphosphates and as agent for polymerization, such as DNA or RNA polymerase or reverse transcriptase) in an appropriate buffer and at a suitable temperature. The length of a primer may vary but typically ranges from 15 to 30 nucleotides. A primer need not match the exact sequence of a template, but must be sufficiently complementary to hybridize with the template.

The term “primer pair” refers to a set of primers including an upstream primer that hybridizes with the 3′ end of the complement of the DNA sequence to be amplified and a downstream primer that hybridizes with the 3′ end of the sequence to be amplified.

The terms “protein”, “polypeptide” and “peptide” are used interchangeably herein when referring to a gene product.

The term “recombinant protein” refers to a polypeptide which is produced by recombinant DNA techniques, wherein generally, DNA encoding the polypeptide is inserted into a suitable expression vector which is in turn used to transform a host cell to produce the heterologous protein.

A “regulatory element”, also termed herein “regulatory sequence” is intended to include elements which are capable of modulating transcription from a 5′ upstream regulatory sequence, including, but not limited to a basic promoter, and include elements such as enhancers and silencers. The term “enhancer”, also referred to herein as “enhancer element”, is intended to include regulatory elements capable of increasing, stimulating, or enhancing transcription from a 5′ upstream regulatory element, including a basic promoter. The term “silencer”, also referred to herein as “silencer element” is intended to include regulatory elements capable of decreasing, inhibiting, or repressing transcription from a 5′ upstream regulatory element, including a basic promoter. Regulatory elements are typically present in 5′ flanking regions of genes. Regulatory elements also may be present in other regions of a gene, such as introns. Thus, it is possible that 5-LO genes have regulatory elements located in introns, exons, coding regions, and 3′ flanking sequences. Such regulatory elements are also intended to be encompassed by the present invention and can be identified by any of the assays that can be used to identify regulatory elements in 5′ flanking regions of genes.

The term “regulatory element” further encompasses “tissue specific” regulatory elements, i.e., regulatory elements which effect expression of an operably linked DNA sequence preferentially in specific cells (e.g., cells of a specific tissue). Gene expression occurs preferentially in a specific cell if expression in this cell type is significantly higher than expression in other cell types. The term “regulatory element” also encompasses non-tissue specific regulatory elements, i.e., regulatory elements which are active in most cell types. Furthermore, a regulatory element can be a constitutive regulatory element, i.e., a regulatory element which constitutively regulates transcription, as opposed to a regulatory element which is inducible, i.e., a regulatory element which is active primarily in response to a stimulus. A stimulus can be, e.g., a molecule, such as a protein, hormone, cytokine, heavy metal, phorbol ester, cyclic AMP (cAMP), or retinoic acid.

Regulatory elements are typically bound by proteins, e.g. transcription factors. The term “transcription factor” is intended to include proteins or modified forms thereof, which interact preferentially with specific nucleic acid sequences, i.e., regulatory elements, and which in appropriate conditions stimulate or repress transcription. Some transcription factors are active when they are in the form of a monomer. Alternatively, other transcription factors are active in the form of a dimer consisting of two identical proteins or different proteins (heterodimer). Modified forms of transcription factors are intended to refer to transcription factors having a postranslational modification, such as the attachment of a phosphate group. The activity of a transcription factor is frequently modulated by a postranslational modification. For example, certain transcription factors are active only if they are phosphorylated on specific residues. Alternatively, transcription factors can be active in the absence of phosphorylated residues and become inactivated by phosphorylation. A list of known transcription factors and their DNA binding site can be found, e.g., in public databases, e.g., TFMATRIX Transcription Factor Binding Site Profile database.

The term “single nucleotide polymorphism” (SNP) refers to a polymorphic site occupied by a single nucleotide, which is the site of variation between allelic sequences. The site is usually preceded by and followed by highly conserved sequences of the allele (e.g., sequences that vary in less than 11100 or 1/1000 members of a population). SNP usually arises due to substitution of one nucleotide for another at the polymorphic site. SNPs can also arise from a deletion of a nucleotide or an insertion of a nucleotide relative to a reference allele. Typically the polymorphic site is occupied by a base other than the reference base. For example, where the reference allele contains the base “T” (thymidine) at the polymorphic site, the altered allele can contain a “C” (cytidine), “G” (guanine), or “A” (adenine) at the polymorphic site.

SNP's may occur in protein-coding nucleic acid sequences, in which case they may give rise to a defective or otherwise variant protein, or genetic disease. Such a SNP may alter the coding sequence of the gene and therefore specify another amino acid (a “missense” SNP) or a SNP may introduce a stop codon (a “nonsense” SNP). When a SNP does not alter the amino acid sequence of a protein, the SNP is called “silent.” SNP's may also occur in noncoding regions of the nucleotide sequence. This may result in defective protein expression, e.g., as a result of alternative spicing, or it may have no effect.

As used herein, the term “specifically hybridizes” or “specifically detects” refers to the ability of a nucleic acid molecule of the invention to hybridize to at least approximately 6, 12, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130 or 140 consecutive nucleotides of either strand of a 5-LO gene.

“5-LO” refers to a 5-lipoxygenase molecule that catalyzes the stereo-specific addition of molecular oxygen to arachidonic acid to form leukotriene A4 (LTA4) (Silverman, et al. 1998) Clin Exp Allergy 5:164; In, et al. (1997) J. Clin. Invest. 99:1130-1137), and therefore functions as a modulator of leukotriene synthesis. It has been found that 5-LO expression is dependent on the presence of a 5′ GC-rich transcription factor binding region; this region contains sequences characteristic of binding motifs, e.g., Sp1 binding motifs (In, et al., supra).

The term “5-LO therapeutic” refers to various forms of 5-LO protein or polypeptides, as well as peptidomimetics, nucleic acids, or small molecules, which can modulate at least one activity of a 5-LO protein by mimicking or potentiating (agonizing) or inhibiting (antagonizing) the effects of a naturally-occurring 5-LO protein. A 5-LO therapeutic which mimics or potentiates the activity of a wild-type 5-LO protein is a “5-LO agonist”. Conversely, a 5-LO therapeutic which inhibits the activity of a wild-type 5-LO protein is a “5-LO antagonist”. 5-LO therapeutics can be used to treat diseases which are associated with a specific 5-LO allele which encodes a protein having an amino acid sequence that differs from that of the wild-type 5-LO protein.

As used herein, the term “transfection” means the introduction of a nucleic acid, e.g., an expression vector, into a recipient cell by nucleic acid-mediated gene transfer. The term “transduction” is generally used herein when the transfection with a nucleic acid is by viral delivery of the nucleic acid. “Transformation”, as used herein, refers to a process in which a cell's genotype is changed as a result of the cellular uptake of exogenous DNA or RNA, and, for example, the transformed cell expresses a recombinant form of a polypeptide or, in the case of anti-sense expression from the transferred gene, the expression of a naturally-occurring form of the recombinant protein is disrupted.

As used herein, the term “transgene” refers to a nucleic acid sequence which has been genetic-engineered into a cell. Daughter cells deriving from a cell in which a transgene has been introduced are also said to contain the transgene (unless it has been deleted). A transgene can encode, e.g., a polypeptide, or an antisense transcript, partly or entirely heterologous, i.e., foreign, to the transgenic animal or cell into which it is introduced, or, is homologous to an endogenous gene of the transgenic animal or cell into which it is introduced, but which is designed to be inserted, or is inserted, into the animal's genome in such a way as to alter the genome of the cell into which it is inserted (e.g., it is inserted at a location which differs from that of the natural gene or its insertion results in a knockout). Alternatively, a transgene can also be present in an episome. A transgene can include one or more transcriptional regulatory sequence and any other nucleic acid, (e.g. intron), that may be necessary for optimal expression of a selected nucleic acid.

A “transgenic animal” refers to any animal, preferably a non-human animal, e.g. a mammal, bird or an amphibian, in which one or more of the cells of the animal contain heterologous nucleic acid introduced by genetic engineering, such as by transgenic techniques well known in the art. The nucleic acid is introduced into the cell, directly or indirectly by introduction into a precursor of the cell, by way of deliberate genetic manipulation, such as by microinjection or by infection with a recombinant virus. The term genetic manipulation does not include classical cross-breeding, or in vitro fertilization, but rather is directed to the introduction of a recombinant DNA molecule. This molecule may be integrated within a chromosome, or it may be extrachromosomally replicating DNA. In the typical transgenic animals described herein, the transgene causes cells to express a recombinant form of one of a protein, e.g. either agonistic or antagonistic forms. However, transgenic animals in which the recombinant gene is silent are also contemplated, as for example, the FLP or CRE recombinase dependent constructs described below. Moreover, “transgenic animal” also includes those recombinant animals in which gene disruption of one or more genes is caused by human intervention, including both recombination and antisense techniques.

The term “treatment,” or “treating” as used herein, is defined as the application or administration of a therapeutic agent to a subject, implementation of lifestyle changes (e.g., changes in diet or environment), administration of medication, or application or administration of a therapeutic agent to a patient who has a disease or disorder, a symptom of disease or disorder or a predisposition toward a disease or disorder, with the purpose to cure, heal, alleviate, relieve, alter, remedy, ameliorate, improve or affect the disease or disorder, the symptoms of the disease or disorder, or the predisposition toward disease.

As used herein, the term “vector” refers to a nucleic acid molecule capable of transporting or replicating another nucleic acid to which it has been linked. One type of preferred vector is an episome, i.e., a nucleic acid capable of extra-chromosomal replication. Preferred vectors are those capable of autonomous replication and/or expression of nucleic acids to which they are linked. Vectors capable of directing the expression of genes to which they are operatively-linked are referred to herein as “expression vectors”. In general, expression vectors of utility in recombinant DNA techniques are often in the form of “plasmids” which refer generally to circular double stranded DNA circles which, in their vector form are not physically linked to the host chromosome. In the present specification, “plasmid” and “vector” are used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors which serve equivalent functions and which become known in the art subsequently hereto.

The term “wild-type allele” refers to an allele of a gene which, when present in two copies in a patient results in a wild-type phenotype. There can be several different wild-type alleles of a specific gene, since certain nucleotide changes in a gene may not affect the phenotype of a patient having two copies of the gene with the nucleotide changes. The terms “wild-type” and “reference sequence” are used interchangeably herein.

Polymorphisms of the Invention

The nucleic acid molecules of the invention include specific 5-LO allelic variants, which differ from the reference sequences set forth in SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, or at least a portion thereof, having a polymorphic region. The preferred nucleic acid molecules of the present invention comprise 5-LO sequences having one or more of the polymorphisms shown in Table 1, those in linkage disequilibrium therewith, and those included in the haplotype, as described herein. Nucleic acid molecules of the invention can function as probes or primers, e.g., in methods for determining the allelic identity of a 5-LO polymorphic region. The nucleic acids of the invention can also be used to determine whether a patient is or is not at risk of developing a disease associated with a specific allelic variant of a 5-LO polymorphic region, e.g., a disease or disorder associated with an aberrant 5-LO activity. The nucleic acids of the invention can further be used to prepare or express 5-LO polypeptides encoded by specific alleles, such as mutant alleles. Such nucleic acids can be used in gene therapy. Polypeptides encoded by specific 5-LO alleles, such as mutant 5-LO polypeptides, can also be used in therapy or for preparing reagents, e.g., antibodies, for detecting 5-LO proteins encoded by these alleles. Accordingly, such reagents can be used to detect mutant 5-LO proteins.

As described herein, several allelic variants of human 5-LO genes have been identified. The invention is intended to encompass all of these allelic variants as well as, those in linkage disequilibrium which can be identified, e.g., according to the methods described herein. “Linkage disequilibrium” refers to an association between specific alleles at two marker loci within a particular population. In general, linkage disequilbrium decreases with an increase in physical distance. If linkage disequilbrium exists between two markers within one gene, then the genotypic information at one marker can be used to make predictions about the genotype of the second marker.

The invention also provides isolated nucleic acids comprising at least one polymorphic region of a 5-LO gene having a nucleotide sequence which differs from the reference nucleotide sequence set forth in SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3. Preferred nucleic acids have a variant allele located in the 5′ upstream regulatory element of the 5-LO gene. Accordingly, preferred nucleic acids of the invention comprise an adenine at residue 1000 of the 5′ upstream regulatory element of GI 187166 (as set forth in SEQ ID NO:4) or a thymine at the corresponding position in an otherwise complementary sequence; a deletion of the nucleotides GTTAAA (SEQ ID NO:62) at residues 472-477 of GI 187166 (as set forth in SEQ ID NO:5) or deletion of the nucleotides TTTAAC at the corresponding positions in an otherwise complementary sequence; and/or an adenine at residue 559 of the 5′ upstream regulatory element of GI 187166 (as set forth in SEQ ID NO:6) or a thymine at the corresponding position in an otherwise complementary sequence.

The nucleic acid molecules of the invention can be single stranded DNA (e.g., an oligonucleotide), double stranded DNA (e.g., double stranded oligonucleotide) or RNA. Preferred nucleic acid molecules of the invention can be used as probes or primers. Primers of the invention refer to nucleic acids which hybridize to a nucleic acid sequence which is adjacent to the region of interest or which covers the region of interest and is extended. As used herein, the term “hybridizes” is intended to describe conditions for hybridization and washing under which nucleotide sequences that are significantly identical or homologous to each other remain hybridized to each other. Preferably, the conditions are such that sequences at least about 70%, more preferably at least about 80%, even more preferably at least about 85% or 90% identical to each other remain hybridized to each other. Such stringent conditions vary according to the length of the involved nucleotide sequence but are known to those skilled in the art and can be found or determined based on teachings in Current Protocols in Molecular Biology, Ausubel et al., eds., John Wiley & Sons, Inc. (1995), sections 2, 4 and 6. Additional stringent conditions and formulas for determining such conditions can be found in Molecular Cloning: A Laboratory Manual, Sambrook et al., Cold Spring Harbor Press, Cold Spring Harbor, N.Y. (1989), chapters 7, 9 and 11. A preferred, non-limiting example of stringent hybridization conditions for hybrids that are at least basepairs in length includes hybridization in 4×sodium chloride/sodium citrate (SSC), at about 65-70° C. (or hybridization in 4×SSC plus 50% formamide at about 42-50° C.) followed by one or more washes in 1×SSC, at about 65-70° C. A preferred, non-limiting example of highly stringent hybridization conditions for such hybrids includes hybridization in 1×SSC, at about 65-70° C. (or hybridization in 1×SSC plus 50% formamide at about 42-50° C.) followed by one or more washes in 0.3×SSC, at about 65-70° C. A preferred, non-limiting example of reduced stringency hybridization conditions for such hybrids includes hybridization in 4×SSC, at about 50-60° C. (or alternatively hybridization in 6×SSC plus 50% formamide at about 40-45° C.) followed by one or more washes in 2×SSC, at about 50-60° C. Ranges intermediate to the above-recited values, e.g., at 65-70° C. or at 42-50° C. are also intended to be encompassed by the present invention. SSPE (1×SSPE is 0.15M NaCl, 10 mM NaH 2 PO 4 , and 1.25 mM EDTA, pH 7.4) can be substituted for SSC (1×SSC is 0.15M NaCl and 15 mM sodium citrate) in the hybridization and wash buffers; washes are performed for 15 minutes each after hybridization is complete.

The hybridization temperature for hybrids anticipated to be less than 50 base pairs in length should be 5-10° C. less than the melting temperature (T m ) of the hybrid, where T m is determined according to the following equations. For hybrids less than 18 base pairs in length, T m (° C.)=2(# of A+T bases)+4(# of G+C bases). For hybrids between 18 and 49 base pairs in length, T m (° C.)=81.5+16.6(log 10 [Na + ])+0.41(%G+C)−(600/N), where N is the number of bases in the hybrid, and [Na + ] is the concentration of sodium ions in the hybridization buffer ([Na + ] for 1×SSC=0.165 M). It will also be recognized by the skilled practitioner that additional reagents may be added to hybridization and/or wash buffers to decrease non-specific hybridization of nucleic acid molecules to membranes, for example, nitrocellulose or nylon membranes, including but not limited to blocking agents (e.g., BSA or salmon or herring sperm carrier DNA), detergents (e.g., SDS), chelating agents (e.g., EDTA), Ficoll, PVP and the like. When using nylon membranes, in particular, an additional preferred, non-limiting example of stringent hybridization conditions is hybridization in 0.25-0.5M NaH 2 PO 4 , 7% SDS at about 65° C., followed by one or more washes at 0.02M NaH2PO 4 , 1% SDS at 65° C., see e.g., Church and Gilbert (1984) Proc. Natl. Acad. Sci. USA 81:1991-1995, (or alternatively 0.2×SSC, 1% SDS).

A primer or probe can be used alone in a detection method, or a primer can be used together with at least one other primer or probe in a detection method. Primers can also be used to amplify at least a portion of a nucleic acid. Probes of the invention refer to nucleic acids which hybridize to the region of interest and which are not further extended. For example, a probe is a nucleic acid which specifically hybridizes to a polymorphic region of a 5-LO gene, and which by hybridization or absence of hybridization to the DNA of a patient or the type of hybrid formed will be indicative of the identity of the allelic variant of the polymorphic region of the 5-LO gene.

Numerous procedures for determining the nucleotide sequence of a nucleic acid molecule, or for determining the presence of mutations in nucleic acid molecules include a nucleic acid amplification step, which can be carried out by, e.g. polymerase chain reaction (PCR). Accordingly, in one embodiment, the invention provides primers for amplifying portions of a 5-LO gene, such as portions of exons and/or portions of introns. In a preferred embodiment, the exons and/or sequences adjacent to the exons of the human 5-LO gene will be amplified to, e.g., detect which allelic variant, if any, of a polymorphic region is present in the 5-LO gene of a patient. Preferred primers comprise a nucleotide sequence complementary a specific allelic variant of a 5-LO polymorphic region and of sufficient length to selectively hybridize with a 5-LO gene. In a preferred embodiment, the primer, e.g., a substantially purified oligonucleotide, comprises a region having a nucleotide sequence which hybridizes under stringent conditions to about 6, 8, 10, or 12, preferably 25, 30, 40, 50, or 75 consecutive nucleotides of a 5-LO gene. In an even more preferred embodiment, the primer is capable of hybridizing to a 5-LO nucleotide sequence and comprises a nucleotide sequence of any sequence set forth in any of SEQ ID NOs:4-6, or complements thereof For example, primers comprising a nucleotide sequence of at least about 15 consecutive nucleotides, at least about 25 nucleotides or having from about 15 to about 20 nucleotides set forth in any of SEQ ID NOs:4-6 or complement thereof are provided by the invention. Primers having a sequence of more than about 25 nucleotides are also within the scope of the invention. Preferred primers of the invention are primers that can be used in PCR for amplifying each of the exons of a 5-LO gene.

Primers can be complementary to nucleotide sequences located close to each other or further apart, depending on the use of the amplified DNA. For example, primers can be chosen such that they amplify DNA fragments of at least about 10 nucleotides or as much as several kilobases. Preferably, the primers of the invention will hybridize selectively to 5-LO nucleotide sequences located about 150 to about 350 nucleotides apart.

For amplifying at least a portion of a nucleic acid, a forward primer (i.e., 5′ primer) and a reverse primer (i.e., 3′ primer) will preferably be used. Forward and reverse primers hybridize to complementary strands of a double stranded nucleic acid, such that upon extension from each primer, a double stranded nucleic acid is amplified. A forward primer can be a primer having a nucleotide sequence or a portion of the nucleotide sequence shown in Table 1 (SEQ ID NOs:4-6) or in SEQ ID NOs.: 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, or 59. A reverse primer can be a primer having a nucleotide sequence or a portion of the nucleotide sequence that is complementary to a nucleotide sequence shown in Table 1 (SEQ ID NOs:4-6) or in SEQ ID NOs.: 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, or 60. Preferred pairs of primers for amplifying each of the exons of human 5-LO are set forth in Table 3 (see Example 2).

Yet other preferred primers of the invention are nucleic acids which are capable of selectively hybridizing to an allelic variant of a polymorphic region of a 5-LO gene. Thus, such primers can be specific for a 5-LO gene sequence, so long as they have a nucleotide sequence which is capable of hybridizing to a 5-LO gene. Preferred primers are capable of specifically hybridizing to any of the allelic variants listed in Table 1 (i e., sequences comprising any of SEQ ID NOs:4-6 or a complement thereof). Such primers can be used, e.g., in sequence specific oligonucleotide priming as described further herein.

The 5-LO nucleic acids of the invention can also be used as probes, e.g., in therapeutic and diagnostic assays. For instance, the present invention provides a probe comprising a substantially purified oligonucleotide, which oligonucleotide comprises a region having a nucleotide sequence that is capable of hybridizing specifically to a region of a 5-LO gene which is polymorphic (i.e., sequences comprising any of SEQ ID NOs:4-6 or a complement thereof). In an even more preferred embodiment of the invention, the probes are capable of hybridizing specifically to one allelic variant of a 5-LO gene having a nucleotide sequence which differs from the nucleotide sequence set forth in SEQ ID NO:1, 2, or 3. Such probes can then be used to specifically detect which allelic variant of a polymorphic region of a 5-LO gene is present in a patient. The polymorphic region can be located in the 5′ upstream regulatory element, exon, or intron sequences of a 5-LO gene.

For example, preferred probes of the invention comprise a sequence listed in Table 2 or a complement thereof, wherein the bold nucleotides represent the location of the nucleotide polymorphism.

Particularly, preferred probes of the invention have a number of nucleotides sufficient to allow specific hybridization to the target nucleotide sequence. Where the target nucleotide sequence is present in a large fragment of DNA, such as a genomic DNA fragment of several tens or hundreds of kilobases, the size of the probe may have to be longer to provide sufficiently specific hybridization, as compared to a probe which is used to detect a target sequence which is present in a shorter fragment of DNA. For example, in some diagnostic methods, a portion of a 5-LO gene may first be amplified and thus isolated from the rest of the chromosomal DNA and then hybridized to a probe. In such a situation, a shorter probe will likely provide sufficient specificity of hybridization. For example, a probe having a nucleotide sequence of about 10 nucleotides may be sufficient.

In preferred embodiments, the probe or primer further comprises a label attached thereto, which, e.g., is capable of being detected, e.g. the label group is selected from amongst radioisotopes, fluorescent compounds, enzymes, and enzyme co-factors.

In a preferred embodiment of the invention, the isolated nucleic acid, which is used, e.g., as a probe or a primer, is modified, so as to be more stable than naturally occurring nucleotides. Exemplary nucleic acid molecules which are modified include phosphoramidate, phosphothioate and methylphosphonate analogs of DNA (see also U.S. Pat. Nos. 5,176,996; 5,264,564; and 5,256,775).

The nucleic acids of the invention can also be modified at the base moiety, sugar moiety, or phosphate backbone, for example, to improve stability of the molecule. The nucleic acids, e.g., probes or primers, may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo), or agents facilitating transport across the cell membrane (see, e.g., Letsinger et al., 1989, Proc. Natl. Acad. Sci. U.S.A. 86;6553-6556; Lemaitre et al., 1987, Proc. Natl. Acad. Sci. 84:648-652; PCT Publication No. WO88/09810, published Dec. 15, 1988), hybridization-triggered cleavage agents. (See, e.g., Krol et al., 1988, BioTechniques 6:958-976) or intercalating agents. (Sec, e.g., Zon, 1988, Pharm. Res. 5:539-549). To this end, the nucleic acid of the invention may be conjugated to another molecule, e.g., a peptide, hybridization triggered cross-inking agent, transport agent, hybridization-triggered cleavage agent, etc.

The isolated nucleic acid comprising a 5-LO intronic sequence may comprise at least one modified base moiety which is selected from the group including but not limited to 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xantine, 4-acetylcytidine, 5-(carboxyhydroxymethyl) uracil, 5-carboxymethylaminomethyl-2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytidine, 5-methylcytidine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5′-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytidine, 5-methyl-2-thiouracil, 2-thiouracil, 4thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methyl-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine.

The isolated nucleic acid may also comprise at least one modified sugar moiety selected from the group including but not limited to arabinose, 2-fluoroarabinose, xylulose, and hexose.

In yet another embodiment, the nucleic acid comprises at least one modified phosphate backbone selected from the group consisting of a phosphorothioate, a phosphorodithioate, a phosphoramidothioate, a phosphoramidate, a phosphordiamidate, a methylphosphonate, an alkyl phosphotriester, and a formacetal or analog thereof.

In yet a further embodiment, the nucleic acid is an α-anomeric oligonucleotide. An α-anomeric oligonucleotide forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual β-units, the strands run parallel to each other (Gautier et al., 1987, Nucl. Acids Res. 15:6625-6641). The oligonucleotide is a 2′-0-methylribonucleotide (Inoue et al., 1987, Nucl. Acids Res. 15:6131-6148), or a chimeric RNA-DNA analogue (Inoue et al., 1987, FEBS Lett. 215:327-330).

Any nucleic acid fragment of the invention can be prepared according to methods well known in the art and described, e.g., in Sambrook, J. Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. For example, discrete fragments of the DNA can be prepared and cloned using restriction enzymes. Alternatively, discrete fragments can be prepared using the Polymerase Chain Reaction (PCR) using primers having an appropriate sequence.

Oligonucleotides of the invention may be synthesized by standard methods known in the art, e.g. by use of an automated DNA synthesizer (such as are commercially available from Biosearch, Applied Biosystems, etc.). As examples, phosphorothioate oligonucleotides may be synthesized by the method of Stein et al. (1988, Nucl. Acids Res. 16:3209), methylphosphonate oligonucleotides can be prepared by use of controlled pore glass polymer supports (Sarin et al., 1988, Proc. Natl. Acad Sci. U.S.A. 85:7448-7451), etc.

The invention also provides vectors and plasmids comprising the nucleic acids of the invention. For example, in one embodiment, the invention provides a vector comprising at least a portion of a 5-LO gene comprising a polymorphic region. Thus, the invention provides vectors for expressing at least a portion of the newly identified allelic variants of the human 5-LO gene reference, as well as other allelic variants, comprising a nucleotide sequence which is different from the nucleotide sequence disclosed in GI 187166, GI 8247778, or GI 450256. The allelic variants can be expressed in eukaryotic cells, e.g., cells of a patient, or in prokaryotic cells.

In one embodiment, the vector comprising at least a portion of a 5-LO allele is introduced into a host cell, such that a protein encoded by the allele is synthesized. The 5-LO protein produced can be used, e.g., for the production of antibodies, which can be used, e.g., in methods for detecting mutant forms of 5-LO. Alternatively, the vector can be used for gene therapy, and be, e.g., introduced into a patient to produce 5-LO protein. Host cells comprising a vector having at least a portion of a 5-LO gene are also within the scope of the invention.

Methods

The invention further provides predictive medicine methods, which are based, at least in part, on the discovery of 5-LO polymorphisms which are associated with specific physiological states and/or diseases or disorders.

For example, information obtained using the diagnostic assays described herein is useful for determining that a patient suffering from an inflammatory or allergy disease or disorder, e.g., asthma, has a more or less severe disease phenotype, e.g., a more or less severe asthma phenotype. Alternatively, the information can be used prognostically for predicting whether a patient will be responsive to treatment of an inflammatory or allergy disease or disorder, including, but not limited to, asthma, with a 5-LO inhibitor or other related agent. Based on the prognostic information, a health care provider can recommend a regimen (e.g., diet or exercise) or therapeutic protocol, (e.g., administration of a 5-LO inhibitor) useful for preventing the particular disease or disorder, or symptoms of the disease or disorder in the patient e.g., asthma.

In addition, knowledge of the identity of a particular 5-LO allele in an individual (the 5-LO genetic profile, including the presence or absence of the polymorphisms of the haplotype as described herein), alone or in conjunction with information on other genetic defects contributing to the same disease (the genetic profile of the particular disease, e.g., asthma) allows customization of therapy for a particular disease to the individual's genetic profile. For example, an individual's 5-LO genetic profile or the genetic profile of a disease or condition associated with a specific allele of a 5-LO polymorphic region, can enable a health care provider: 1) to more effectively prescribe a drug that will address the molecular basis of the disease or condition (e.g., a 5-LO inhibitor as described in U.S. Pat. Nos. 5,703,093, 5,750,565, or 6,025,384); and 2) to better determine the appropriate dosage of a particular drug. For example, the expression level of 5-LO proteins, alone or in conjunction with the expression level of other genes, known to contribute to the same disease, can be measured in many patients at various stages of the disease to generate a transcriptional or expression profile of the disease. Expression patterns of individual patients can then be compared to the expression profile of the disease to determine the appropriate drug and dose to administer to the patient.

The ability to target populations expected to show the highest clinical benefit, e.g., subgroups of asthmatic populations which respond or do not respond to specific therapies, e.g., treatment with 5-LO inhibitors, or subgroups of asthmatic populations with more or less severe asthma phenotypes, based on the 5-LO or disease genetic profile, can enable: 1) the repositioning of marketed drugs with disappointing market results; 2) the rescue of drug candidates whose clinical development has been discontinued as a result of safety or efficacy limitations, which are patient subgroup-specific; and 3) an accelerated and less costly development for drug candidates and more optimal drug labeling (e.g., since the use of 5-LO as a marker is useful for optimizing effective dose).

These and other methods are described in further detail in the following sections.

A. Prognostic and Diagnostic Assays

The present methods provide means for determining if a patient has a disease, condition or disorder that is associated a specific 5-LO allele, e.g., aberrant, e.g., decreased, eosinophil levels, or the severity of an inflammatory disease or disorder phenotype, e.g. the severity of an asthma phenotype among an asthmatic population. The present methods also provide means for predicting or determining if a patient will be more or less responsive to treatment with a 5-LO inhibitor, based on determination of a specific 5-LO allele.

The present invention provides methods for determining the molecular structure of a 5-LO gene, such as a human 5-LO gene, or a portion thereof. For example, the present invention provides methods for determining the presence or absence of the haplotype described herein. In one embodiment, determining the molecular structure of at least a portion of a 5-LO gene comprises determining the identity of the allelic variant of at least one polymorphic region of a 5-LO gene (determining the presence or absence of one or more of the allelic variants, or their complements, of SEQ ID NOs.:4-6, thereby determining the presence of the haplotype as described herein). A polymorphic region of a 5-LO gene can be located in an exon, an intron, at an intron/exon border, or, preferably, in the 5′ upstream regulatory element of the 5-LO gene.

The invention provides methods for determining whether a patient has a specific disease or disorder phenotype, e.g., asthma phenotype, associated with a specific allelic variant of a polymorphic region of a 5-LO gene. Such disease phenotypes are associated with aberrant 5-LO activity, e.g., aberrant 5-LO expression, or increased or decreased eosinophil counts in the patient. Aberrant 5-LO protein level can result from aberrant transcription or post transcriptional regulation. Thus, allelic differences in specific regions of a 5-LO gene result in differences of 5-LO protein due to differences in regulation of expression. In particular, some of the identified polymorphisms in the human 5-LO gene are associated with differences in the level of transcription, RNA maturation, splicing, or translation of the 5-LO gene or transcription product. For example, the presence of a variant Sp1 binding site, which is associated with the haplotype of the invention, result in reduced levels of 5-LO expression. Therefore, presence of any of the polymorphisms of the haplotype predicts a reduced levels of 5-LO expression.

Analysis of one or more 5-LO polymorphic regions in a patient, including the identities of the polymorphisms included in the haplotype of the present invention can be useful for predicting or determining whether a patient has more or less severe inflammatory disease phenotype, e.g., a more or less severe asthma phenotype, associated with increased or decreased eosinophil levels resulting in more or less severe symptoms of the inflammatory disease or disorder.

In preferred embodiments, the methods of the invention can be characterized as comprising detecting, in a sample of cells or nucleic acid from the patient, the presence or absence of a specific allelic variant of one or more polymorphic regions of a 5-LO gene. The allelic differences can be: (i) a difference in the identity of at least one nucleotide or (ii) a difference in the number of nucleotides, which difference can be a single nucleotide or several nucleotides. The invention also provides methods for detecting differences in 5-LO genes such as chromosomal rearrangements, e.g., chromosomal dislocation. The invention can also be used in prenatal diagnostics.

A preferred detection method is allele specific hybridization using probes overlapping the polymorphic site and having about 5, 10, 20, 25, or 30 nucleotides around the polymorphic region. Examples of probes for detecting specific allelic variants of the polymorphic region located in the 5′ upstream regulatory element of the 5-LO gene are probes comprising a nucleotide sequence set forth in any of SEQ ID NOs.:64, 65, and 66 or the complement thereof. In a preferred embodiment of the invention, several probes capable of hybridizing specifically to allelic variants are attached to a solid phase support, e.g., a “chip”. Oligonucleotides can be bound to a solid support by a variety of processes, including lithography. For example a chip can hold up to 250,000 oligonucleotides (GeneChip, Affymetrix). Mutation detection analysis using these chips comprising oligonucleotides, also termed “DNA probe arrays” is described e.g., in Cronin et al. (1996) Human Mutation 7:244. In one embodiment, a chip comprises all the allelic variants of at least one polymorphic region of a gene. The solid phase support is then contacted with a test nucleic acid and hybridization to the specific probes is detected. Accordingly, the identity of numerous allelic variants of one or more genes can be identified in a simple hybridization experiment. For example, the identity of the allelic variant of the nucleotide polymorphism in the 5′ upstream regulatory element can be determined in a single hybridization experiment.

In other detection methods, it is necessary to first amplify at least a portion of a 5-LO gene prior to identifying the allelic variant. Amplification can be performed, e.g., by PCR and/or LCR (see Wu and Wallace, (1989) Genomics 4:560), according to methods known in the art. In one embodiment, genomic DNA of a cell is exposed to two PCR primers and amplification for a number of cycles sufficient to produce the required amount of amplified DNA. In preferred embodiments, the primers are located between 150 and 350 base pairs apart. Preferred primers, such as primers for amplifying each of the exons of the human 5-LO gene, are listed in Table 3.

Alternative amplification methods include: self sustained sequence replication (Guatelli, J. C. et al., 1990, Proc. Natl. Acad. Sci. USA 87:1874-1878), transcriptional amplification system (Kwoh, D. Y. et al., 1989, Proc. Natl. Acad. Sci. USA 86:1173-1177), Q-Beta Replicase (Lizardi, P. M. et al., 1988, Bio/Technology 6:1197), and self-sustained sequence replication (Guatelli et al, (1989) Proc. Nat. Acad. Sci. 87:1874), and nucleic acid based sequence amplification (NABSA), or any other nucleic acid amplification method, followed by the detection of the amplified molecules using techniques well known to those of skill in the art. These detection schemes are especially useful for the detection of nucleic acid molecules if such molecules are present in very low numbers.

In one embodiment, any of a variety of sequencing reactions known in the art can be used to directly sequence at least a portion of a 5-LO gene and detect allelic variants, e.g., mutations, by comparing the sequence of the sample sequence with the corresponding wild-type (control) sequence. Exemplary sequencing reactions include those based on techniques developed by Maxam and Gilbert ( Proc. Natl Acad Sci USA ( 1977) 74:560) or Sanger (Sanger et al (1977) Proc. Nat. Acad. Sci 74:5463). It is also contemplated that any of a variety of automated sequencing procedures may be utilized when performing the subject assays ( Biotechniques (1995) 19:448), including sequencing by mass spectrometry (see, for example, U.S. Pat. No. 5,547,835 and international patent application Publication Number WO 94/16101, entitled DNA Sequencing by Mass Spectrometry by H. Köster, U.S. Pat. No. 5,547,835 and international patent application Publication Number WO 94/21822 entitled “DNA Sequencing by Mass Spectrometry Via Exonuclease Degradation” by H. Köster), and U.S. Pat. No. 5,605,798 and International Patent Application No. PCT/US96/03651 entitled DNA Diagnostics Based on Mass Spectrometry by H. Köster;. Cohen et al. (1996) Adv Chromatogr 36:127-162; and Griffin et al. (1993) Appl Biochem Biotechnol 38:147-159). It will be evident to one skilled in the art that, for certain embodiments, the occurrence of only one, two or three of the nucleic acid bases need be determined in the sequencing reaction. For instance, A-track or the like, e.g., where only one nucleotide is detected, can be carried out.

Yet other sequencing methods are disclosed, e.g., in U.S. Pat. No. 5,580,732 entitled “Method of DNA sequencing employing a mixed DNA-polymer chain probe” and U.S. Pat. No. 5,571,676 entitled “Method for mismatch-directed in vitro DNA sequencing”.

In some cases, the presence of a specific allele of a 5-LO gene in DNA from a patient can be shown by restriction enzyme analysis. For example, a specific nucleotide polymorphism can result in a nucleotide sequence comprising a restriction site which is absent from the nucleotide sequence of another allelic variant.

In a further embodiment, protection from cleavage agents (such as a nuclease, hydroxylamine or osmium tetroxide and with piperidine) can be used to detect mismatched bases in RNA/RNA DNA/DNA, or RNA/DNA heteroduplexes (Myers, et al. (1985) Science 230:1242). In general, the technique of “mismatch cleavage” starts by providing heteroduplexes formed by hybridizing a control nucleic acid, which is optionally labeled, e.g., RNA or DNA, comprising a nucleotide sequence of a 5-LO allelic variant with a sample nucleic acid, e.g., RNA or DNA, obtained from a tissue sample. The double-stranded duplexes are treated with an agent which cleaves single-stranded regions of the duplex such as duplexes formed based on basepair mismatches between the control and sample strands. For instance, RNA/DNA duplexes can be treated with RNase and DNA/DNA hybrids treated with S1 nuclease to enzymatically digest the mismatched regions. In other embodiments, either DNA/DNA or RNA/DNA duplexes can be treated with hydroxylamine or osmium tetroxide and with piperidine in order to digest mismatched regions. After digestion of the mismatched regions, the resulting material is then separated by size on denaturing polyacrylamide gels to determine whether the control and sample nucleic acids have an identical nucleotide sequence or in which nucleotides they are different. See, for example, Cotton et al (1988) Proc. Natl Acad Sci USA 85:4397; Saleeba et al (1992) Methods Enzymol. 217:286-295. In a preferred embodiment, the control or sample nucleic acid is labeled for detection.

In another embodiment, an allelic variant can be identified by denaturing high-performance liquid chromatography (DHPLC) (Oefner and Underhill, (1995) Am. J. Human Gen. 57: Suppl. A266). DHPLC uses reverse-phase ion-pairing chromatography to detect the heteroduplexes that are generated during amplification of PCR fragments from individuals who are heterozygous at a particular nucleotide locus within that fragment (Oefner and Underhill (1995) Am. J. Human Gen. 57: Suppl. A266). In general, PCR products are produced using PCR primers flanking the DNA of interest. DHPLC analysis is carried out and the resulting chromatograms are analyzed to identify base pair alterations or deletions based on specific chromatographic profiles (see O'Donovan et al. (1998) Genomics 52:44-49).

In other embodiments, alterations in electrophoretic mobility is used to identify the type of 5-LO allelic variant. For example, single strand conformation polymorphism (SSCP) may be used to detect differences in electrophoretic mobility between mutant and wild type nucleic acids (Orita et al. (1989) Proc Natl. Acad. Sci USA 86:2766, see also Cotton (1993) Mutat Res 285:125-144; and Hayashi (1992) Genet Anal Tech Appl 9:73-79). Single-stranded DNA fragments of sample and control nucleic acids are denatured and allowed to renature. The secondary structure of single-stranded nucleic acids varies according to sequence, the resulting alteration in electrophoretic mobility enables the detection of even a single base change. The DNA fragments may be labeled or detected with labeled probes. The sensitivity of the assay may be enhanced by using RNA (rather than DNA), in which the secondary structure is more sensitive to a change in sequence. In another preferred embodiment, the subject method utilizes heteroduplex analysis to separate double stranded heteroduplex molecules on the basis of changes in electrophoretic mobility (Keen et al. (1991) Trends Genet 7:5).

In yet another embodiment, the identity of an allelic variant of a polymorphic region is obtained by analyzing the movement of a nucleic acid comprising the polymorphic region in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (DGGE) (Myers et al (1985) Nature 313:495). When DGGE is used as the method of analysis, DNA will be modified to insure that it does not completely denature, for example by adding a GC clamp of approximately 40 bp of high-melting GC-rich DNA by PCR. In a further embodiment, a temperature gradient is used in place of a denaturing agent gradient to identify differences in the mobility of control and sample DNA (Rosenbaum and Reissner (1987) Biophys Chem 265:1275).

Examples of techniques for detecting differences of at least one nucleotide between 2 nucleic acids include, but are not limited to, selective oligonucleotide hybridization, selective amplification, or selective primer extension. For example, oligonucleotide probes may be prepared in which the known polymorphic nucleotide is placed centrally (allele-specific probes) and then hybridized to target DNA under conditions which permit hybridization only if a perfect match is found (Saiki et al. (1986) Nature 324:163); Saiki et al (1989) Proc. Natl Acad. Sci USA 86:6230; and Wallace et al. (1979) Nucl. Acids Res. 6:3543). Such allele specific oligonucleotide hybridization techniques may be used for the simultaneous detection of several nucleotide changes in different polylmorphic regions of 5-LO. For example, oligonucleotides having nucleotide sequences of specific allelic variants are attached to a hybridizing membrane and this membrane is then hybridized with labeled sample nucleic acid. Analysis of the hybridization signal will then reveal the identity of the nucleotides of the sample nucleic acid.

Alternatively, allele specific amplification technology which depends on selective PCR amplification may be used in conjunction with the instant invention. Oligonucleotides used as primers for specific amplification may carry the allelic variant of interest in the center of the molecule (so that amplification depends on differential hybridization) (Gibbs et al (1989) Nucleic Acids Res. 17:2437-2448) or at the extreme 3′ end of one primer where, under appropriate conditions, mismatch can prevent, or reduce polymerase extension (Prossner (1993) Tibtech 11:238; Newton et al. (1989) Nucl. Acids Res. 17:2503). This technique is also termed “PROBE” for Probe Oligo Base Extension. In addition it may be desirable to introduce a novel restriction site in the region of the mutation to create cleavage-based detection (Gasparini et at (1992) Mol. Cell Probes 6:1).

In another embodiment, identification of the allelic variant is carried out using an oligonucleotide ligation assay (OLA), as described, e.g. in U.S. Pat. No. 4,998,617 and in Landegren, U. et al., (1988) Science 241:1077-1080. The OLA protocol uses two oligonucleotides which are designed to be capable of hybridizing to abutting sequences of a single strand of a target. One of the oligonucleotides is linked to a separation marker, e.g., biotinylated, and the other is detectably labeled. If the precise complementary sequence is found in a target molecule, the oligonucleotides will hybridize such that their termini abut, and create a ligation substrate. Ligation then permits the labeled oligonucleotide to be recovered using avidin, or another biotin ligand. Nickerson, D. A. et al. have described a nucleic acid detection assay that combines attributes of PCR and OLA (Nickerson, D. A. et al., (1990) Proc. Natl. Acad. Sci. (U.S.A) 87:8923-8927. In this method, PCR is used to achieve the exponential amplification of target DNA, which is then detected using OLA.

Several techniques based on this OLA method have been developed and can be used to detect specific allelic variants of a polymorphic region of a 5-LO gene. For example, U.S. Pat. No. 5,593,826 discloses an OLA using an oligonucleotide having 3′-amino group and a 5′-phosphorylated oligonucleotide to form a conjugate having a phosphoramidate linkage. In another variation of OLA described in Tobe et al. ((1996) Nucleic Acids Res 24: 3728), OLA combined with PCR permits typing of two alleles in a single microtiter well. By marking each of the allele-specific primers with a unique hapten, i.e., digoxigenin and fluorescein, each OLA reaction can be detected by using hapten specific antibodies that are labeled with different enzyme reporters, alkaline phosphatase or horseradish peroxidase.

This system permits the detection of the two alleles using a high throughput format that leads to the production of two different colors.

The invention further provides methods for detecting single nucleotide polymorphisms in a 5-LO gene. Because single nucleotide polymorphisms constitute sites of variation flanked by regions of invariant sequence, their analysis requires no more than the determination of the identity of the single nucleotide present at the site of variation and it is unnecessary to determine a complete gene sequence for each patient. Several methods have been developed to facilitate the analysis of such single nucleotide polymorphisms.

In one embodiment, the single base polymorphism can be detected by using a specialized exonuclease-resistant nucleotide, as disclosed, e.g., in Mundy, C. R. (U.S. Pat. No. 4,656,127). According to the method, aprimer complementary to the allelic sequence immediately 3′ to the polymorphic site is permitted to hybridize to a target molecule obtained from a particular animal or human. If the polymorphic site on the target molecule contains a nucleotide that is complementary to the particular exonuclease-resistant nucleotide derivative present, then that derivative will be incorporated onto the end of the hybridized primer. Such incorporation renders the primer resistant to exonuclease, and thereby permits its detection. Since the identity of the exonuclease-resistant derivative of the sample is known, a finding that the primer has become resistant to exonucleases reveals that the nucleotide present in the polymorphic site of the target molecule was complementary to that of the nucleotide derivative used in the reaction. This method has the advantage that it does not require the determination of large amounts of extraneous sequence data.

In another embodiment of the invention, a solution-based method is used for determining the identity of the nucleotide of a polymorphic site. Cohen, D. et al. (French Patent 2,650,840; PCT Appln. No. W091/02087). As in the Mundy method of U.S. Pat. No. 4,656,127, a primer is employed that is complementary to allelic sequences immediately 3′ to a polymorphic site. The method determines the identity of the nucleotide of that site using labeled dideoxynucleotide derivatives, which, if complementary to the nucleotide of the polymorphic site will become incorporated onto the terminus of the primer.

An alternative method, known as Genetic Bit Analysis or GBA™ is described by Goelet, P. et al. (PCT Appln. No. 92/15712). The method of Goelet, P. et al. uses mixtures of labeled terminators and a primer that is complementary to the sequence 3′ to a polymorphic site. The labeled terminator that is incorporated is thus determined by, and complementary to, the nucleotide present in the polymorphic site of the target molecule being evaluated. In contrast to the method of Cohen et al. (French Patent 2,650,840; PCT Appln. No. WO91/02087) the method of Goelet, P. et al. is preferably a heterogeneous phase assay, in which the primer or the target molecule is immobilized to a solid phase.

Recently, several primer-guided nucleotide incorporation procedures for assaying polymorphic sites in DNA have been described (Komher, J. S. et al., Nucl. Acids. Res. 17:7779-7784 (1989); Sokolov, B. P., Nucl. Acids Res. 18:3671 (1990); Syvanen, A.-C., et al., Genomics 8:684-692 (1990); Kuppuswamy, M. N. et al., Proc. Natl. Acad. Sci. ( U.S.A. ) 88:1143-1147 (1991); Prezant, T. R. et al., Hum. Mutat. 1:159-164 (1992); Ugozzoli, L. et al., GATA 9:107-112 (1992); Nyren, P. et al., Anal. Biochem. 208:171-175 (1993)). These methods differ from GBA™ in that they all rely on the incorporation of labeled deoxynucleotides to discriminate between bases at a polymorphic site. In such a format, since the signal is proportional to the number of deoxynucleotides incorporated, polymorphisms that occur in runs of the same nucleotide can result in signals that are proportional to the length of the run (Syvanen, A.-C., et al., Amer. J. Hum. Genet. 52:46-59 (1993)).

If a polymorphic region is located in an exon, either in a coding or non-coding portion of the gene, the identity of the allelic variant can be determined by determining the molecular structure of the mRNA, pre-mRNA, or cDNA. The molecular structure can be determined using any of the above described methods for determining the molecular structure of the genomic DNA, e.g., see Example 1.

The methods described herein may be performed, for example, by utilizing pre-packaged diagnostic kits, such as those described above, comprising at least one probe or primer nucleic acid described herein, which may be conveniently used, e.g., to determine whether a patient has a specific disease phenotype associated with a specific 5-LO allelic variant.

Sample nucleic acid to be analyzed by any of the above-described diagnostic and prognostic methods can be obtained from any cell type or tissue of a patient. For example, a patient's bodily fluid (e.g., blood) can be obtained by known techniques (e.g. venipuncture). Alternatively, nucleic acid tests can be performed on dry samples (e.g. hair or skin). Fetal nucleic acid samples can be obtained from maternal blood as described in International Patent Application No. WO91/07660 to Bianchi. Alternatively, amniocytes or chorionic villi may be obtained for performing prenatal testing.

Diagnostic procedures may also be performed in situ directly upon tissue sections (fixed and/or frozen) of patient tissue obtained from biopsies or resections, such that no nucleic acid purification is necessary. Nucleic acid reagents may be used as probes and/or primers for such in situ procedures (see, for example, Nuovo, G. J., 1992, PCR in situ hybridization: protocols and applications, Raven Press, NY).

In addition to methods which focus primarily on the detection of one nucleic acid sequence, profiles may also be assessed in such detection schemes. Fingerprint profiles may be generated, for example, by utilizing a differential display procedure, Northern analysis and/or RT-PCR.

B. Pharmacogenomics

Knowledge of the identity of the allele of one or more 5-LO gene polymorphic regions in a patient (the 5-LO genetic profile), alone or in conjunction with information on other genetic defects contributing to the same disease (the genetic profile of the particular disease, including, for example, the determination of the presence of the haplotype described herein) also allows a customization of the therapy for a particular disease to the patient's genetic profile.

It has been determined that the presence of a variant Sp1 binding site in the promoter region of the 5-LO gene results in reduced responsiveness to treatment with particular therapies, including treatment with 5-LO inhibitors, or other agents which antagonize 5-LO in asthmatic patients (U.S. Pat. No. 6,090,547). Therefore, based on the existence of the haplotype as described herein which is in linkage disequilibrium with any one of three variant Sp1 binding site, the detection in an asthmatic patient of an allele present in at least one of the polymorphic sites included in the haplotype of the present invention can be used to predict whether or not that the patient has reduced responsiveness to treatment with a specific treatment, e.g., treatment with a 5-LO inhibitor. The methods of the invention further provide methods for identifying a candidate for treatment with a 5-LO inhibitor by the detection in a patient of an allele present in at least one of the polymorphic sites included in the haplotype of the present invention, or a complement thereof.

For example, patients having a specific allele of a 5-LO gene may or may not exhibit symptoms of a particular disease or be predisposed to developing symptoms of a particular disease. Further, if those patients are symptomatic, they may or may not respond well to a certain drug, e.g., a specific 5-LO therapeutic, such as a 5-LO inhibitor, but may respond to another. Thus, generation of a 5-LO genetic profile (e.g., categorization of alterations in 5-LO genes which are associated with the development of a particular disease, including determination of the presence of the haplotype described herein), from a population of patients, who are symptomatic for a disease or condition that is caused by or contributed to by a defective and/or aberrantly expressed 5-LO gene and/or protein (a 5-LO genetic population profile) and comparison of an individual's 5-LO profile to the population profile, permits the selection or design of drugs that are expected to be safe and efficacious for a particular patient or patient population (i.e., a group of patients having the same genetic alteration).

For example, a 5-LO population profile can be performed by determining the 5-LO profile, e.g., the identity of 5-LO alleles, in particular the identity of 5-LO alleles included in the haplotype as described herein, in a patient population having a disease, which is associated with one or more specific alleles of 5-LO polymorphic regions. Optionally, the 5-LO population profile can further include information relating to the response of the population to a 5-LO therapeutic, using any of a variety of methods, including, monitoring: 1) the severity of symptoms associated with the 5-LO related disease, 2) 5-LO gene expression level, 3) 5-LO mRNA level, 4) 5-LO protein level, 5) eosinophil level, and/or 6) leukotriene level, and dividing or categorizing the population based on particular 5-LO alleles. The 5-LO genetic population profile can also, optionally, indicate those particular 5-LO alleles which are present in patients that are either responsive or non-responsive to a particular therapeutic, e.g., a 5-LO inhibitor. This information or population profile, is then useful for predicting which individuals should respond to particular drugs, based on their individual 5-LO profile.

In a preferred embodiment, the 5-LO profile is a transcriptional or expression level profile and is comprised of determining the expression level of 5-LO proteins, alone or in conjunction with the expression level of other genes known to contribute to the same disease at various stages of the disease.

Pharmacogenomic studies can also be performed using transgenic animals. For example, one can produce transgenic mice, e.g., as described herein, which contain a specific allelic variant of a 5-LO gene. These mice can be created, e.g., by replacing their wild-type 5-LO gene with an allele of the human 5-LO gene. The response of these mice to specific 5-LO therapeutics can then be determined.

C. Monitoring Effects of 5-LO Therapeutics During Clinical Trials

The present invention provides a method for monitoring the effectiveness of treatment of a patient with an agent (e.g., an agonist, antagonist peptidomimetic, protein, peptide, nucleic acid, small molecule, or other drug candidate identified, e.g., by the screening assays described herein) comprising the steps of (i) obtaining a preadministration sample from a patient prior to administration of the agent; (ii) detecting the level of expression or activity of a 5-LO protein, mRNA or gene in the preadministration sample; (iii) obtaining one or more post-administration samples from the patient; (iv) detecting the level of expression or activity of the 5-LO protein, mRNA or gene in the post-administration samples; (v) comparing the level of expression or activity of the 5-LO protein, mRNA, or gene in the preadministration sample with those of the 5-LO protein, mRNA, or gene in the post administration sample or samples; and (vi) altering the administration of the agent to the patient accordingly. For example, increased administration of the agent may be desirable to increase the expression or activity of 5-LO to higher levels than detected, i.e., to increase the effectiveness of the agent. Alternatively, decreased administration of the agent may be desirable to decrease expression or activity of 5-LO to lower levels than detected, i.e., to decrease the effectiveness of the agent.

Cells of a patient may also be obtained before and after administration of a 5-LO therapeutic to detect the level of expression of genes other than 5-LO, to verify that the 5-LO therapeutic does not increase or decrease the expression of genes which could be deleterious. This can be done, e.g., by using the method of transcriptional profiling. Thus, mRNA from cells exposed in vivo to a 5-LO therapeutic and mRNA from the same type of cells that were not exposed to the 5-LO therapeutic could be reverse transcribed and hybridized to a chip containing DNA from numerous genes, to thereby compare the expression of genes in cells treated and not treated with a 5-LO therapeutic. If, for example a 5-LO therapeutic turns on the expression of a proto-oncogene in an individual, use of this particular 5-LO therapeutic may be undesirable.

D. Methods of Treatment

The present invention provides for both prophylactic and therapeutic methods of treating a patient having a disorder associated with specific 5-LO alleles and/or aberrant 5-LO expression or activity, e.g., inflammatory or allergy diseases or disorders, such as asthma.

i) Prophylactic Methods

In one aspect, the invention provides a method for preventing in a patient, a disease or condition associated with a specific 5-LO allele such as an inflammatory or allergy disease or disorder, e.g., asthma, and medical conditions resulting therefrom, by administering to the patient an agent which counteracts the unfavorable biological effect of the specific 5-LO allele. Subjects at risk for such a disease can be identified by a diagnostic or prognostic assay, e.g., as described herein. Administration of a prophylactic agent can occur prior to the manifestation of symptoms associated with specific 5-LO alleles, such that a disease or disorder is prevented or, alternatively, delayed in its progression. Depending on the identity of the 5-LO allele in a patient, a compound that counteracts the effect of this allele is administered. The compound can be a compound modulating the activity of 5-LO, e.g., a 5-LO inhibitor. The treatment can also be a specific diet, or environmental alteration. In particular, the treatment can be undertaken prophylactically, before any other symptoms are present. Such a prophylactic treatment could thus prevent the development of an aberrant inflammatory response, e.g., asthma. The prophylactic methods are similar to therapeutic methods of the present invention and are further discussed in the following subsections.

(ii) Therapeutic Methods

The invention further provides methods of treating patients having a specific disease or disorder phenotype associated with a specific allelic variant of a polymorphic region of a 5-LO gene, e.g., a more moderate or more severe phenotype. Preferred diseases or disorders include those associated with aberrant leukotriene synthesis, eosinophil levels, and 5-LO expression, and disorders resulting therefrom.

In one embodiment, the method comprises (a) determining the identity of one or more allelic variants of the invention; and (b) administering to the patient a compound that compensates for the effect of the specific allelic variant. The polymorphic region can be localized at any location of the gene, e.g., in a regulatory element (e.g. in a 5′ upstream regulatory element), in an exon, (e.g., coding region of an exon), in an intron, or at an exon/intron border. Thus, depending on the site of the polymorphism in the 5-LO gene, a patient having a specific variant of the polymorphic region which is associated with a specific disease or condition, can be treated with compounds which specifically compensate for the effect of the allelic variant.

In a preferred embodiment, the identity of any one of the polymorphisms included in the haplotype is determined. In another preferred embodiment, the identity of one or more of the following nucleotides of a 5-LO gene of a patient is determined: adenine at residue 1000 of the reference sequence GI 187166, a deletion at residues 472-477 (GTTAAA) (SEQ ID NO:62) of the reference sequence GI 187166, and/or adenine at residue 559 of the reference sequence GI 187166 may be determined.

In another preferred embodiment, the identity of at least one of the nucleotides listed above is determined, in combination with one or more of the known nucleotide polymorphisms from Table 1, i.e., adenine at residue 94 of the reference sequence GI 187166, adenine at residue 137 of the reference sequence GI 187166, and/or variant Sp1 binding site e.g., sites containing three, four, or six Sp1 motifs, may be determined. In a particularly preferred embodiment, it is determined that a patient has one or more of the following: an adenine at residue 1000 of the reference sequence GI 187166, a deletion of residues 472-477 (GTTAAA) (SEQ ID NO:62) of the reference sequence GI 187166, an adenine at residue 559 of the reference sequence GI 187166, in combination with one or more of the following previously known polymorphisms, preferably an adenine at residue 84 of GI 187166, and/or an adenine at residue 137 of GI 187166, or a variant Sp1 binding site

If a patient having an inflammatory or allergy disease or disorder, e.g., asthma, has one or more of the polymorphisms of the invention (as set forth in Table 1), or one or more of the polymorphisms of the invention in combination with one or more of the previously known polymorphisms listed in Table 1 or a variant Sp1 binding site allele, that patient has a decreased risk of having high eosinophil levels, has a more moderate inflammatory disease phenotype, has a decreased risk of having increased 5-LO expression, and can be identified as having decreased responsiveness to a 5-LO inhibitor, as compared to a patient without one of the aforementioned polymorphisms.

Similarly, if a patient having an inflammatory disease or disorder, e.g., asthma does not have one or more of the polymorphisms of the invention (as set forth in Table 1), or one or more of the polymorphisms of the invention in combination with one or more of the previously known polymorphisms listed in Table 1 or a variant Sp1 binding site, that patient has an increased risk of having increased eosinophil levels, an increased risk of having a more severe inflammatory disease phenotype, an increased risk of having increased 5-LO expression, and can be identified as having increased responsiveness to a 5-LO inhibitor as compared to a patient with one of the aforementioned polymorphisms.

Generally, the allelic variant can be a mutant allele, i.e., an allele which when present in one, or preferably two copies, in a patient results in a change in the phenotype of the patient. A mutation can be a substitution, deletion, and/or addition of at least one nucleotide relative to the wild-type allele (i.e., the reference sequence). Depending on where the mutation is located in the 5-LO gene, the patient can be treated to specifically compensate for the mutation. For example, if the mutation is present in the coding region of the gene and results in a more active 5-LO protein, the patient can be treated, e.g., by administration to the patient of a 5-LO inhibitor, such that the administration of the inhibitor prevents aberrant leukotriene synthesis by the 5-LO protein. Normal 5-LO protein can also be used to counteract or compensate for the endogenous mutated form of the 5-LO protein. Normal 5-LO protein can be directly delivered to the patient or indirectly by gene therapy wherein some cells in the patient are transformed or transfected with an expression construct encoding wild-type 5-LO protein. Nucleic acids encoding wild-type human 5-LO protein are set forth in SEQ ID NOs.:1, 2, and 3 (GI Accession Nos. 187166, 8247778 and 4502056).

Furthermore, depending on the site of the mutation in the 5-LO protein and the specific effect on its activity, specific treatments can be designed to compensate for that effect. The 5-LO protein is a lipoxygenase which interacts with arachidonic acid to synthesize leukotrienes (LTs), e.g., LTA4, B4, C4, D4, and E4. The structure of the 5-LO protein is further described, e.g., in Silverman, et al. (1999) Proc. Assoc. Am. Physicians 111 (6):525-36 and Matsunoto, et al. (1988) supra). Thus, if the mutation results in a 5-LO protein which causes as increased ability of 5-LO to synthesize leukotrienes, resulting in an accumulation of leukotrienes in the patient, a treatment can be designed which removes excess leukotrienes from the patient or inhibits 5-LO from synthesis of leukotrienes. In one embodiment, a compound which inhibits 5-LO synthesis of leukotrienes is administered to the patient. For example, a compound described in U.S. Pat. No. 5,703,093, 5,750,565, or 6,025,384 can be administered to the patient.

Yet in another embodiment, the invention provides methods for treating a patient having a mutated 5-LO gene, in which the mutation is located in a regulatory region of the gene. Such a regulatory region can be localized in the 5′ upstream regulatory element of the gene, in the 5′ or 3′ untranslated region of an exon, or in an intron. A mutation in a regulatory region can result in increased production of 5-LO protein, decreased production of 5-LO protein, or production of 5-LO having an aberrant tissue distribution. The effect of a mutation in a regulatory region upon the 5-LO protein can be determined, e.g., by measuring the 5 -LO protein level or mRNA level in cells having a 5-LO gene having this mutation and which, normally (i.e., in the absence of the mutation) produce 5-LO protein. The effect of a mutation can also be determined in vitro. For example, if the mutation is in the 5′ upstream regulatory element, a reporter construct can be constructed which comprises the mutated 5′ upstream regulatory element linked to a reporter gene, the construct transfected into cells, and comparison of the level of expression of the reporter gene under the control of the mutated 5′ upstream regulatory element and under the control of a wild-type 5′ upstream regulatory element. Such experiments can also be carried out in mice transgenic for the mutated 5′ upstream regulatory element. If the mutation is located in an intron, the effect of the mutation can be determined, e.g., by producing transgenic animals in which the mutated 5-LO gene has been introduced and in which the wild-type gene may have been knocked out. Comparison of the level of expression of 5-LO in the mice transgenic for the mutant human 5-LO gene with mice transgenic for a wild-type human 5-LO gene will reveal whether the mutation results in increased, or decreased synthesis of the 5-LO protein and/or aberrant tissue distribution of 5-LO protein. Such analysis could also be performed in cultured cells, in which the human mutant 5-LO gene is introduced and, e.g., replaces the endogenous wild-type 5-LO gene in the cell. Thus, depending on the effect of the mutation in a regulatory region of a 5-LO gene, a specific treatment can be administered to a patient having such a mutation. Accordingly, if the mutation results in decreased 5-LO protein levels, the patient can be identified as being less responsive to treatment with a 5-LO inhibitor. For example, the presence of a non-wild-type Sp1 binding site allele or a polymorphism included in a haplotype as described herein, may indicate reduced levels of 5-LO expression, reduced eosinophil levels, a less severe asthma phenotype, and reduced responsiveness to 5-LO inhibition by a 5-LO inhibitor, e.g., a 5-LO inhibitor described in U.S. Pat. No. 5,703,093, 5,750,565 or 6,025,384.

A correlation between drug responses and specific alleles of 5-LO can be shown, for example, by clinical studies wherein the response to specific drugs of patients having different allelic variants of a polymorphic region of a 5-LO gene is compared. Such studies can also be performed using animal models, such as mice having various alleles of human 5-LO genes and in which, e.g., the endogenous 5-LO has been inactivated such as by a knock-out mutation. Test drugs are then administered to the mice having different human 5-LO alleles and the response of the different mice to a specific compound is compared. Accordingly, the invention provides assays for identifying the drug which will be best suited for treating a specific disease or condition in a patient. For example, it will be possible to select drugs which will be devoid of toxicity, or have the lowest level of toxicity possible for treating a patient having a disease or condition.

Other Uses for the Nucleic Acid Molecules of the Invention

The identification of different alleles of 5-LO can also be useful for identifying an individual among other individuals from the same species. For example, DNA sequences can be used as a fingerprint for detection of different individuals within the same species (Thompson, J. S. and Thompson, eds., Genetics in Medicine, WB Saunders Co., Philadelphia, Pa. (1991)). This is useful, for example, in forensic studies and paternity testing, as described below.

A. Forensics

Determination of which specific allele occupies a set of one or more polymorphic sites in an individual identifies a set of polymorphic forms that distinguish the individual from others in the population. See generally National Research Council, The Evaluation of Forensic DNA Evidence (Eds. Pollard et al., National Academy Press, DC, 1996). The more polymorphic sites that are analyzed, the lower the probability that the set of polymorphic forms in one individual is the same as that in an unrelated individual. Preferably, if multiple sites are analyzed, the sites are unlinked. Thus, the polymorphisms of the invention can be used in conjunction with known polymorphisms in distal genes. Preferred polymorphisms for use in forensics are biallelic because the population frequencies of two polymorphic forms can usually be determined with greater accuracy than those of multiple polymorphic forms at multi-allelic loci.

The capacity to identify a distinguishing or unique set of polymorphic markers in an individual is useful for forensic analysis. For example, one can determine whether a blood sample from a suspect matches a blood or other tissue sample from a crime scene by determining whether the set of polymorphic forms occupying selected polymorphic sites is the same in the suspect and the sample. If the set of polymorphic markers does not match between a suspect and a sample, it can be concluded (barring experimental error) that the suspect was not the source of the sample. If the set of markers is the same in the sample as in the suspect, one can conclude that the DNA from the suspect is consistent with that found at the crime scene. If frequencies of the polymorphic forms at the loci tested have been determined (e.g., by analysis of a suitable population of individuals), one can perform a statistical analysis to determine the probability that a match of suspect and crime scene sample would occur by chance.

p(ID) is the probability that two random individuals have the same polymorphic or allelic form at a given polymorphic site. For example, in biallelic loci, four genotypes are possible: AA, AB, BA, and BB. If alleles A and B occur in a haploid genome of the organism with frequencies x and y, the probability of each genotype in a diploid organism is (see WO 95/12607):

Homozygote: p ( AA )= x 2

Homozygote: p ( BB )= y 2 =(1 −x ) 2

 Single Heterozygote: p ( AB )= p ( BA )= xy=x (1 −x )

Both Heterozygotes: p ( AB+BA )=2 xy= 2 x (1 −x )

The probability of identity at one locus (i.e., the probability that two individuals, picked at random from a population will have identical polymorphic forms at a given locus) is given by the equation: p(ID)=(x 2 ).

These calculations can be extended for any number of polymorphic forms at a given locus. For example, the probability of identity p(ID) for a 3-allele system where the alleles have the frequencies in the population of x, y, and z, respectively, is equal to the sum of the squares of the genotype frequencies: P(ID)=x 4 +(2xy) 2 +(2yz) 2 +(2xz) 2 +z 4 +y 4 .

In a locus of n alleles, the appropriate binomial expansion is used to calculate p(ID) and p(exc).

The cumulative probability of identity (cum p(ID)) for each of multiple unlinked loci is determined by multiplying the probabilities provided by each locus:

cum p (ID)= p (DI 1 ) p (ID 2 ) p (ID) . . . p (IDn).

The cumulative probability of non-identity for n loci (i.e., the probability that two random individuals will be difference at 1 or more loci) is given by the equation:

cum p (nonID)=1 −cum p (ID).

If several polymorphic loci are tested, the cumulative probability of non-identity for random individuals becomes very high (e.g., one billion to one). Such probabilities can be taken into account together with other evidence in determining the guilt or innocence of the suspect.

B. Paternity Testing

The object of paternity testing is usually to determine whether a male is the father of a child. In most cases, the mother of the child is known, and thus, it is possible to trace the mother's contribution to the child's genotype. Paternity testing investigates whether the part of the child's genotype not attributable to the mother is consistent to that of the puntative father. Paternity testing can be performed by analyzing sets of polymorphisms in the putative father and in the child.

If the set of polymorphisms in the child attributable to the father does not match the set of polymorphisms of the putative father, it can be concluded, barring experimental error, that that putative father is not the real father. If the set of polymorphisms in the child attributable to the father does match the set of polymorphisms of the putative father, a statistical calculation can be performed to determine the probability of a coincidental match.

The probability of parentage exclusion (representing the probability that a random male will have a polymorphic form at a given polymorphic site that makes him incompatible as the father) is given by the equation (see WO 95/12607): p(exc)=xy(1-xy), where x and y are the population frequencies of alleles A and B of a biallelic polymorphic site.

(At a triallelic site p(exc)=xy(1−xy)+yz(1−yz)+xz(1−xz)+3xyz(1−xyz)), where x, y, and z and the respective populations frequencies of alleles A, B, and C).

The probability of non-exclusion is: p(non-exc)=1−p(exc).

The cumulative probability of non-exclusion (representing the values obtained when n loci are is used) is thus:

Cum p (non-exc)= p (non-exc1) p (non-exc2) p (non-exc3) . . . p (non-excn).

The cumulative probability of the exclusion for n loci (representing the probability that a random male will be excluded: cum p(exc)=1−cum p(non-exc).

If several polymorphic loci are included in the analysis, the cumulative probability of exclusion of a random male is very high. This probability can be taken into account in assessing the liability of a putative father whose polymorphic marker set matches the child's polymorphic marker set attributable to his or her father.

Kits

As set forth herein, the invention provides methods, e.g., diagnostic and therapeutic methods, e.g., for determining the type of allelic variant of a polymorphic region present in a 5-LO gene, such as a human 5-LO gene. In preferred embodiments, the methods use probes or primers comprising nucleotide sequences which are complementary polymorphic region of a 5-LO gene (SEQ ID NOs:4-6). In a more preferred embodiment, the methods are used to determine the identity of one or more allelic variants in polymorphic region which is included in the haplotype described herein. Accordingly, the invention provides kits for performing these methods.

In a preferred embodiment, the invention provides a kit for determining whether a patient has a more moderate or more severe inflammatory or allergy disease phenotype associated with a specific allelic variant of a 5-LO polymorphic region. In an even more preferred embodiment, the disease or disorder is characterized by an abnormal 5-LO activity, e.g., aberrant 5-LO expression. In an even more preferred embodiment, the inflammatory or allergy disease is, e.g., asthma, lung inflammation, nephritis, amyloidosis, rheumatoid arthritis, chronic bronchitis, sarcoidosis, scleroderma, lupus, polymyositis, Reiter's syndrome, psoriasis, pelvic inflammatory disease, orbital inflammatory disease, thrombotic disease, and inappropriate allergic responses to environmental stimuli such as poison ivy, pollen, insect stings and certain foods, including atopic dermatitis and contact dermatitis.

A preferred kit provides reagents for determining whether a patient will or will not be responsive to treatment of a disease or disorder associated with a specific allelic variant of a polymorphic region of a 5-LO gene, e.g., asthma, with, for example, a 5-LO inhibitor. In a preferred embodiment, the inflammatory disease is, e.g., asthma, lung inflammation, nephritis, amyloidosis, rheumatoid arthritis, chronic bronchitis, sarcoidosis, scleroderma, lupus, polymyositis, Reiter's syndrome, psoriasis, pelvic inflammatory disease, orbital inflammatory disease, thrombotic disease, and inappropriate allergic responses to environmental stimuli such as poison ivy, pollen, insect stings and certain foods, including atopic dermatitis and contact dermatitis. In a preferred embodiment, the kit of the invention can be used in selecting the appropriate drug to administer to a patient suffering from an inflammatory disease or disorder.

Preferred kits comprise at least one probe or primer which is capable of specifically hybridizing under stringent conditions to a 5-LO sequence or polymorphic region and instructions for use. The kits preferably comprise at least one of the above described nucleic acids. Preferred kits for amplifying at least a portion of a 5-LO gene, e.g., the 5′ upstream regulatory element, comprise two primers, at least one of which is capable of hybridizing to an allelic variant sequence. Even more preferred kits comprise a pair of primers selected from the group consisting of SEQ ID NO: 9 and SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12, SEQ ID NO: 13 and SEQ ID NO: 14, SEQ ID NO: 15 and SEQ ID NO: 16 SEQ ID NO: 17and SEQ ID NO: 18, SEQ ID NO: 19 and SEQ ID NO: 20, SEQ ID NO: 21 and SEQ ID NO: 22, SEQ ID NO: 23 and SEQ ID NO: 24, SEQ ID NO: 25 and SEQ ID NO: 26, SEQ ID NO: 27 and SEQ ID NO: 28, SEQ ID NO: 29 and SEQ ID NO: 30, SEQ ID NO: 31 and SEQ ID NO: 32, SEQ ID NO: 33 and SEQ ID NO: 34, SEQ ID NO: 35 and SEQ ID NO: 36, SEQ ID NO: 37 and SEQ ID NO: 38, SEQ ID NO: 39 and SEQ ID NO: 40, SEQ ID NO: 41 and SEQ ID NO: 42, SEQ ID NO: 43 and SEQ ID NO: 44, SEQ ID NO: 45 and SEQ ID NO: 46, SEQ ID NO: 47 and SEQ ID NO: 48, SEQ ID NO: 49 and SEQ ID NO: 50, SEQ ID NO: 51 and SEQ ID NO: 52, SEQ ID NO: 53 and SEQ ID NO: 54, SEQ ID NO: 55 and SEQ ID NO: 56, SEQ ID NO: 57 and SEQ ID NO: 58, and SEQ ID NO: 59 and SEQ ID NO: 60 (see Table 3).

The kits of the invention can also comprise one or more control nucleic acids or reference nucleic acids, such as nucleic acids comprising a 5-LO intronic sequence. For example, a kit can comprise primers for amplifying a polymorphic region of a 5-LO gene and a control DNA corresponding to such an amplified DNA and having the nucleotide sequence of a specific allelic variant. Thus, direct comparison can be performed between the DNA amplified from a patient and the DNA having the nucleotide sequence of a specific allelic variant. In one embodiment, the control nucleic acid comprises at least a portion of a 5-LO gene of an individual who does not have an inflammatory disease, aberrant synthesis of leukotrienes, decreased eosinophil levels, or a disease or disorder associated with an aberrant 5-LO activity.

Yet other kits of the invention comprise at least one reagent necessary to perform the assay. For example, the kit can comprise an enzyme. Alternatively the kit can comprise a buffer or any other necessary reagent.

The present invention is further illustrated by the following examples which should not be construed as limiting in any way. The contents of all cited references (including, without limitation, literature references, issued patents, published patent applications as well as the Figures, Tables, and database references) as cited throughout this application are hereby expressly incorporated by reference. The practice of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, transgenic biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example, Molecular Cloning A Laboratory Manual, 2nd Ed., ed. by Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory Press: 1989); DNA Cloning, Volumes I and II (D. N. Glover ed., 1985); Oligonucleotide Synthesis (M. J. Gait ed., 1984); Mullis et al. U.S. Pat. No. 4,683,195; Nucleic Acid Hybridization (B. D. Hames & S. J. Higgins eds. 1984); Transcription And Translation (B. D. Hames & S. J. Higgins eds. 1984); Culture Of Animal Cells (R. I. Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells And Enzymes (IRL Press, 1986); B. Perbal, A Practical Guide To Molecular Cloning (1984); the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); Gene Transfer Vectors For Mammalian Cells (J. H. Miller and M. P. Calos eds., 1987, Cold Spring Harbor Laboratory); Methods In Enzymology, Vols. 154 and 155 (Wu et al. eds.), Immunochemical Methods In Cell And Molecular Biology (Mayer and Walker, eds., Academic Press, London, 1987); Handbook Of Experimental Immunology, Volumes I-IV (D. M. Weir and C. C. Blackwell, eds., 1986); Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).

EXAMPLES

Example 1

Determination of the Genomic Structure of the 5-LO Gene

This example describes the determination of the genomic structure of the 5-LO gene. Identification of the various functional regions of the gene, including, but not limited to, 5′ and 3′ untranslated regions (UTR) and intron/exon boundaries was necessary for subsequent variant detection experiments. Two sequence comparison software applications were used to elucidate genomic structure of the 5-LO gene. Both applications involve the comparison of the cDNA sequence of the 5-LO gene to the sequence of large genomic DNA clones (bacterial artificial chromosomes or BACs) that encode the 5-LO gene in it's untranscribed form. One application is the Basic Local Alignment Search Tool™ or BLAST™ (Altschul et al. (1990) J. Mol. Biol. 215(3):403-410; Altschul et al. (1993) Nature Genetics 3:266-272; Altschul et al. (1997) Nuc. Acids Res. 25:3389-3402; Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA 87:2267-2268). The other application is Sequencher 3.1.1 (Gene Codes Corporation). Although based on different algorithms, these applications aid in the determination of genomic structure by the same principle.

These applications allow the comparison of two or more different nucleotide sequences (in the case of BLAST™, amino acid sequences can also be aligned) and identification or alignment of regions of high similarity or identity. Aligning cDNA sequence of the 5-LO gene with the corresponding BAC sequences with either tool allows visualization of regions of identity between the transcribed sequence and the sequence of the genomic region from which it was derived. Regions of sequence identity between the two sources are exonic regions and can be confirmed by the presence of known conserved splice elements flanking them. Regions of sequence contained in the BAC sequences but not in the cDNA sequence are considered to be either intronic sequence or lay outside of the 5′ and 3′ boundaries of the gene.

Once the genomic structure of the 5-LO gene, e.g., intron/exon boundaries, was determined, PCR primers were designed for use with variant nucleotide detection experiments. Exonic sequences (including intron/exon junctions), 5′UTR, 3′UTR and approximately of 1 kbp upstream of the transcription start site were scanned for variant nucleotide detection as described in Examples 2, 3, 4, and 5.

Example 2

Identification of Primer Pairs to Isolate Intronic, Exonic, and 5′ Upstream Regulatory Element Sequences for Detection of Polymorphisms and Mutations

Multiple pairs of primers were synthesized in order to amplify each of the exons or portions thereof and adjacent intronic regions. Genomic DNA from a human subject was subjected to PCR in 25 μl reactions (1×PCR Amplitaq polymerase buffer, 0.1 mM dNTPs, 0.8 μM 5′ primer, 0.8 μM 3′ primer, 0.75 units of Amplitaq polymerase, 50 ng genomic DNA) using each of the above described pairs of primers under the following cycle conditions: 94° C. for 2 min, 35×[94° C. for 40 sec, 57° C. for 30 sec, 72° C. for 1 min], 72° C. for 5 min, 4° C. hold. The resulting PCR products were analyzed on a 2% agarose gel. The identity of the PCR product was confirmed by digestion with a restriction enzyme and subsequent agarose electrophoresis. Twenty-three pairs of oligomers were chosen to serve as PCR primers to amplify regions containing each of the 14 coding exons of the human 5-LO gene and eleven pairs of primers were chosen to serve as PCR primers to amplify the 5′ upstream regulatory element. The nucleotide sequences of the forward and reverse primers are indicated in Table 3, as well as the SEQ ID NOs for each primer and the location of the primers (e.g., exon, 3′UTR, or 5′UTR/5′ upstream regulatory element) within the 5-LO gene. The expected sizes of the PCR products are also set forth in Table 3. In addition, polymorphism ID numbers corresponding to those listed in Table 1 are included where there was a polymorphism identified with a particular primer pair.

Example 3

Detection of Polymorphic Regions in the Human 5-LO Gene by DHPLC

This example describes the use of denaturing high performance liquid chromatography (DHPLC) for the identification of DNA sequence variations.

DNA samples for these experiments were obtained from a population of 144 individuals to be used for polymorphism discovery. These 144 DNA samples included DNA samples from a population of forty-eight North American Caucasian individuals, forty-eight African American individuals, and forty-eight individuals from throughout the Anhui province in East Central China. Furthermore, the forty-eight DNA samples from the Chinese individuals are from unrelated control subjects, obtained at random, and have been assessed for a number of traits related to asthma and allergies. Among the primary variables of interest are lung function, a response to “skin-prick” tests, physician's diagnosis of asthma, total serum IgE, and peripheral blood eosinophils. The peripheral blood eosinophil count was measured using the Coulter counter technique as described in Barnard D F, et al. (1989) Clin Lab Haematol 11(3):255-66, and is expressed in terms of eosinophils per microliter.

DHPLC uses reverse-phase ion-pairing chromatography to detect the heteroduplexes that are generated during amplification of PCR fragments from individuals who are heterozygous at a particular nucleotide locus within that fragment (Oefner and Underhill (1995) Am. J. Human Gen. 57:Suppl. A266).

Generally, the analysis was carried out as described in O'Donovan et al. ((1998) Genomics 52:44-49). PCR products having product sizes ranging from about 150-400 bp were generated using the primers and PCR conditions described in Example 2. Two PCR reactions were pooled together for DHPLC analysis (4 ul of each reaction for a total of 8 ul per sample). DHPLC was performed on a DHPLC system purchased from Transgenomic, Inc. The gradient was created by mixing buffers A (0.1M TEAA) and B (0.1M TEAA, 25% Acetontitrile). WAVEmaker™ software was utilized to predict a melting temperature and calculate a buffer gradient for mutation analysis of a given DNA sequence. The resulting chromatograms were analyzed to identify base pair alterations or deletions based on specific chromatographic profiles.

Example 4

Detection of Polymorphic Regions in the Human 5-LO Gene by SSCP

Genomic DNA from each of the 144 individuals described in Example 3 was subjected to PCR in 25 μl reactions (1×PCR Amplitaq polymerase buffer, 0.1 mM dNTPs, 0.8 μM 5′ primer, 0.8 μM 3′ primer, 0.75 units of Amplitaq polymerase, 50 ng genomic DNA) using each of the above described pairs of primers under the following cycle conditions: 94° C. for 2 min, 35×[94° C. for 40 sec, 57° C. for 30 sec, 72° C. for 1 min], 72° C. 5 min, 4° C. hold. The expected sizes of the PCR products, are also indicated in Table 3.

The amplified genomic DNA fragments were then analyzed by SSCP (Orita et al. (1989) PNAS USA 86:2766, see also Cotton (1993) Mutat Res 285:125-144; and Hayashi (1992) Genet Anal Tech Appl 9:73-79). From each 25 μl PCR reaction, 3 μl was taken and added to 7 μl of loading buffer. The mixture was heated to 94° C. for 5 min and then immediately cooled in a slurry of ice-water. 3-4 μl were then loaded on a 10% polyacrylamide gel either with 10% glycerol or without 10% glycerol, and then subjected to electrophoresis either overnight at 4 Watts at room temperature, overnight at 4 Watts at 4° C. (for amplifying a 5′ upstream regulatory element), or for 5 hours at 20 Watts at 4° C. The secondary structure of single-stranded nucleic acids varies according to sequence, thus allowing the detection of small differences in nucleic acid sequence between similar nucleic acids. At the end of the electrophoretic period, the DNA was analyzed by gently overlaying a mixture of dyes onto the gel (1× the manufacturer's recommended concentration of SYBR GREEN I™ and SYBR GREEN II™ in 0.5×TBE buffer (MOLECULAR PROBES™)) for 5 min, followed by rinsing in distilled water and detection in a FLUOROIMAGER 575™ (MOLECULAR DYNAMICS™).

Example 5

Identification of Polymorphic Regions in the Human 5-LO Gene by Direct Sequencing of PCR Products

To determine the sequences of the polymorphisms identified, the regions containing the polymorphisms were reamplified using the aforementioned primers. The genomic DNA from the subjects was subjected to PCR in 50 μl reactions (1×PCR Amplitaq polymerase buffer, 0.1 mM dNTPs, 0.8 μM 5′ primer, 0.8 μM 3′ primer, 0.75 units of Amplitaq polymerase, 50 ng genomic DNA) using each of the above described pairs of primers under the following cycle conditions: 94° C. for 2 min, 35×[94° C. for 40 sec, 57° C. for 30 sec, 72° C. for 1 min], 72° C. 5 min, 4° C. hold. The newly amplified products were then purified using the Qiagen Qiaquick™ PCR purification kit according to the manufacturer's protocol, and subjected to sequencing using the aforementioned primers which were utilized for amplification.

The results indicate that there is a change from a guanine to an adenine in the 5′ upstream regulatory element of the 5-LO gene at residue 1000 of the reference sequence GI 187166 (polymorphism ID No. 5loprr1). A second polymorphism is a deletion in the 5′ upstream regulatory element at residues 472477 of the reference sequence GI 187166 (polymorphism ID No. 5lo01a). A third polymorphism is a change from a guanine to an adenine in the 5′ upstream regulatory element at residue 559 of the reference sequence GI 187166 (polymorphism ID No. 5lo04a). These polymorphisms are listed in Table 1 and correspond to SEQ ID NOs: 4-6.

Example 6

Identification of a Variant Haplotype in the Promoter Region of 5-LO

Three populations comprised of individuals of different racial backgrounds, i.e., North American Caucasian, Asian Chinese, and African American, were screened for variants in the promoter region of 5-LO using the methods described herein. It was found that polymorphism Id Nos. 5loprr1, 5lo01a, 5lo04a, 5lonrra, and 5lonrrb all segregate together such that when an individual has one of these polymorphisms, all five polymorphisms are present. This five polymorphism haplotype is conserved at an identical frequency between these three different racial backgrounds (North American Caucasian, Asian Chinese, and African American). The five polymorphisms (polymorphism Id Nos. 5loprr1, 5lo01a, 5lo04a, 5lonrra, and 5lonrrb) are in complete linkage disequilibrium and are present at a frequency of approximately 18% in all three populations screened. Moreover the haplotype was in linkage disequilibrium with any one of the three variant 5-LO Sp-1 binding sites. In Caucasian and Chinese populations, the haplotype was associated with the variant Sp1 binding site having four Sp1 motifs. In the African American population, the haplotype was found to be associates with any one of the three variant Sp1 binding sites (i.e., binding sites having 3, 4 or 6 Sp1 motifs).

Example 7

Association of 5-LO Variants with Eosinophil Levels in Chinese Asthmatic Families

This example describes the determination of the association between the presence of one or more polymorphisms, low eosinophil levels in a sample of Chinese asthmatic families.

Method

A study of 275 families was conducted to determine whether the variant identified as “Polymorphism ID No. 5lo01a” (see Table 1) is associated with asthma-related phenotypes. Selected individuals from these families were genotyped at the 5lo01a polymorphism. Two statistical tests were then conducted to determine whether evidence exists of an association between this locus and asthma-related phenotypes.

For this study, genomic DNA was isolated from peripheral blood lymphocytes using the Puregene™ kit (Gentra Systems Inc.™), according to manufacturer's suggested protocol. SSCP analysis (see Example 4) was used to determine each subjects' genotype at the promoter deletion/insertion polymorphism 5lo01a (e.g., the deletion/insertion of (GTTAAA) (SEQ ID NO:62).

To determine whether genotype is associated with peripheral blood eosinophil count (an index of asthmatic disease), counts (per ml) were compared between individuals carrying at least one copy of the deletion polymorphism (e.g., those with at least one allele that is missing the residues “GTTAAA” (SEQ ID NO:62)), and those carrying no copies of the polymorphism. One individual was selected from each family; the selections were based solely on genotype, and were designed to make the sizes of these two comparison groups as similar as possible. A Student T-TEST was used to determine whether the difference in mean eosinophil count between these two groups was significant.

Results

For the first test, 138 unrelated individuals with at least one copy of the deletion were analyzed, as were 137 unrelated individuals with no copies of the deletion. The mean eosinophil level among individuals with the deletion was 222.17 (standard deviation=246.38); the mean among individuals without the deletion was 324.09 (standard deviation=482.75). The Student T-TEST comparing the mean eosinophil level between these two groups revealed that there is a significant difference (p=0.029) between these means.

Discussion

An association test was performed to determine whether a 5-LO promoter variant is associated with asthma-related phenotypes. Specifically, it was examined whether genotype at the 5-LO polymorphism associates with peripheral blood eosinophil counts. The test gave evidence that such an association exists. The test demonstrated that eosinophil counts are significantly lower among individuals carrying at least one copy of the rare deletion allele at this locus. Thus, the promoter genotype can be used predict whether an individual has a greater chance of having a abnormally low eosinophil count.

Equivalents

Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

66 1 2189 DNA Homo sapiens 1ggatccagaa taaccaaaac aatattgaaa aataaagaac agcgttggtg gattaacatt 60ttccaatttc aaaacttact atagcactgc ggtaatcaag cagtgtggca ctgtatagca 120tgtacattac agatcagtgg actagaatca atgtccagaa ataaaccgtt atgtttataa 180tgaattactt tttaataagg tgtcaagaca acgcaatggg aaaagaataa tgaattcaac 240aaatgatgca tggacaaccg gacatgcaca tgcaacacaa tgaatttgaa ttcttctatc 300gctccatgca taaaaactaa ctcaaaatgg gtcacggatg taaatgaaaa gctaaaacta 360taataatcct agaggaaaac ctaggagtaa atctttaaga tgttattgta ggcagtggtt 420tctcagatag gaccccaaaa tcacaagcga caaaaagaaa ttggacttaa agttaaatac 480ttttgtgctt caaacatcat caagaaagtg aaaacacaac ccgcagaagc aataaaaatg 540tctgtaagtc atgtatccga ttagagactt ctatccagga tatataaata atgcaattca 600atgataaaaa agataaatag cccagttttc caaagagtca agcatctgaa tatacatctc 660tccaaaaata tacagatatc caacaagcat gtgaaaagat gttcaaagcc atttgccagg 720tgcacaaacc caagacagta tgaggagatg ctacagggac tctgctgctt cacagacatg 780aagcgttggt gagaatgtag gcagccgcct ttggggactt cacatccccg ccgccccacg 840cacggtgagc tagtgtttaa acttagccga gatcaataca cgcgactgtg tgcccgtcag 900accctgcgct gccggcgggg ctgggagagg cgggcgccag gagtgggcgg gaacctgggg 960gtcaggcccc agccgcggga agcgcgccca ggagcgcgcg aaaccttctc cacacccttc 1020caggcatttg cccgccgcga ttcagagagc cgacccgtga cccctggcct cccctagaca 1080gccccgcatg tccagatgtg ccgtcccgcc tgcctcccgc gaccactggc catctctggg 1140cctgggcgcg gttctcggcg cccggcctgc ccccgccagg agccgcaggt ccagccagtg 1200aagaagcccg cgcctgaagg agcctctgtg ctccagaatc catcctcagt atcagcgctg 1260gggtggcctc ctccaggaag cccttctgat tctctcatgg gtcgctcttc ctctgcagac 1320tcccggagca ccccctgctc caagtaccgc aagtggcact gagaacttgg ggagagcaga 1380ggctgtgcct agatttgtag ggagtccccg cagctccacc ccagggccta caggagcctg 1440gccttgggcg aagccgaggc aggcaggcag ggcaaagggt ggaagcaatt caggagagaa 1500cgagtgaacg aatggatgag gggtggcagc cgaggttgcc ccagtcccct ggctgcagga 1560acagacacct cgctgaggag agacccagga gcgaggcccc tgccccgccc gaggcgaggt 1620cccgcccagt cggcgccgcg cgtgaagagt gggagagaag tactgcgggg gcgggggcgg 1680gggcgggggc gggggcgggg gcagccggga gcctggagcc agaccggggc ggggccggga 1740ccggggccag ggaccagtgg tgggaggagg ctgcggcgct agatgcggac acctggaccg 1800ccgcgccgag gctcccggcg ctcgctgctc ccgcggcccg cgccatgccc tcctacacgg 1860tcaccgtggc cactggcagc cagtggttcg ccggcactga cgactacatc tacctcagcc 1920tcgtgggctc ggcgggctgc agcgagaagc acctgctgga caagcccttc tacaacgact 1980tcgagcgtgg cgcggtgagc gcgggcgggg cacgggtgga gcgcgggctg aggtgcgtcc 2040gggacccggt ttggacggca gaggcctggg cgggggcgcc gagggcccgt cggggcggcc 2100cggacaggac tgggggtgtc caggaccctg tcagggaggg cagaactgcg gtggggcgtg 2160ccctgggctc ccagtggccg gtgggtacc 2189 2 168273 DNA Homo sapiens misc_feature (1)...(168273) n = A,T,C or G 2ttgcacgctn ctcagccccg gctgtactat tggtctacac gtgccatcgt ggtttgctgc 60acccatcaac ccatcatcta cattaggtat ttctcctaat gctctctttc cccttgcccc 120ccaccctcta acacgccctg ttgtgtgatg ttgacctccc tgtgtacatg tgttgtcatt 180gtacaactcc tgcttatgag tgacaacatc tgtctgattt tctgtcttgt gttagtttgc 240tgagaatgat ggttcccatc ttcatccttg tacctgcaaa ggacatgaac tcatccttta 300atatggctgc ttagaatccc acagtataga tgtgcctcat tttctttatc cagactatca 360ttgatgggca tttgggttgg atccaagact ttgctattgt gaacagtgat tcaataaaca 420tacgagtgca tgtgtcttta tggatgaatg attgataatc ctttgggcat atacccacta 480atgggattgc tggacctaat ggtttgcctg gatcctgatc cttgaggaat caccacactc 540tcttacagtg taaaagtgtc tctatatata ctcatcccct ccagcatctg ttatttactg 600actctttaat catcaacctt cttactggag agagaaggtt taattaccac taatgacgag 660cctttaatca aattgttgtt ggacccttcc caacttactt gcgaaggggt catttgtaaa 720cttacgccac cttttgatgg ggcagttttt ctcgtttatc cgcaaagatc ctgggggacc 780ccttttttta actcttttga agaccgttat atctgaacaa tttctcccat accgagaggt 840ggcgggttac aacccttgaa agtttctttt tttttttggg gccccttagt ttcacaaaaa 900tcccttcgga ccaatttgcc ttttgttgcc aaggaatttt tccccctaag tcactaggaa 960tttttcccag ggcacacagg tgtagtggcc ttccccagaa tttttctcct gggtatccaa 1020tgttataaga aaaaaaattg gggaattttt ttaaacctcc aataaaattt tttatagaga 1080taaggccggt ttcatttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc 1200ggtcttcagc ccgtggtgaa gactgattaa cagtcataag aaggtaaagc tctaagtgac 1260ctgtaagaaa catacagtct cacacaatta ggcaaggagt ccaacttgga agaaagagtg 1320gcatagattc aaaccctgat gccacaactt atgttaaccc tcctgaggct caacttctca 1380cacatgacag gagaatctca tcatgtacct caattaattg atatacagat tatggtaaac 1440attcaatgaa aaagtatgta aagtttctat cattctacta cagttggtat tcaacaaatg 1500ccactttatt cactttctgt taagcctgaa agaagtactg aatgacatca acgagtgcaa 1560ttcaacaaat ataatttgag cactgattac tcaataagca ttatgaggaa ctaaaatatt 1620atcagcaaat gatggctgac atagaggaaa tgtgacagag taggaagatc cctgtactcc 1680ctgtatcaaa ggactgagat tcttccactc aggaccatgt gtgaccccaa cagaaagtag 1740aaatttacag aaagatacct ggcaaaacac ccaaatagtt tgaagttaaa aaatatacct 1800ctgaataaca ccccatggaa atttttaaag attataatgt gaattaaaat gaaaacatat 1860aaccataaca aaaattgtag gatacaggaa aactggtgct catatagcat taactgttta 1920cattacattt tagagaatca acttaattca attatctaag tttctatttt agaaaactat 1980gtaaaaaaag gagagcatat ttaaaaagta agcagaagaa caaaagataa gcatgacaga 2040caatgaaatt gaaaatggaa aaatcataga gaagaatcaa atgaatcaaa agtagtttct 2100tggaaaagat caataaataa aattggttga tataatttac tgatattagg agtgggaaag 2160ataatcactg atcatagatc catgatgaac aagcttattt caataaatta ataaactcag 2220caacttcaac aaaatggaca gattacttgg aagacaccaa ttaccaaagc tcaaaaaaag 2280agaatctaaa tagtcctata tttatgaaaa aaaaaaacaa aactgaattt gtatttaaaa 2340actttgccac aaagaaaatt ttaggttcag atgggtttcc taataaattt ttcccattta 2400gtaagaataa tatcaattct acacaaactc attcaaaaaa aaaggagaga gaatacttta 2460caacacgttc tgcaaggtca gtattaccct gataccaaaa caaaggatat tacataaaaa 2520cgacaccaat atccttcatg aacatagatt cttcaccacg gggctggaag gatccgnnnn 2580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatag cacgcccttg agcccctggt 2700gaatataaga gattaggcta cagcgtgttc tactccaact ttatttgctt tctgagcatc 2760taattccact ttgcaaaatg gacatttttt ttccctcttg agagtcagga aattaccagg 2820tgtgccagga taatccttag tatcaactgt aaaatatcag tataaaataa tgagtaagca 2880tatttcagtg ggtgtttata tgcttttggt tgcatagcat gagccaaagg cagataaaaa 2940atgtgggctc cagatagtga ctcaatctcc agaaatcata atgtcagtta cacaatgata 3000tttctatatt ggcatcacaa aataatccac ttgtcatagg ataattgctc tagatgaggg 3060catggagctg tgctgaggaa tctgccattt ttctttatcc agactctgtc tcataccgac 3120cacaggcccc aatcattgtc tatcaaaaaa catgcttgtg tctcttttgg cttactatga 3180aaggaagcaa gatcttttcc tcaagcattt aagtgttgac tgtgggctac ccagtgtgct 3240agacactgng gatcaaattc tgtgaggtta aaaatacccc ataactcact ggatagaaat 3300tcaaaatgct cgctatgttg attctatgat caacttccaa ggggaccttt ntacctccta 3360tcttcatcag caagcaacta cattgatttt gtagcccagg atctattccc acgtcaaccg 3420tgggtttcaa atgtttgctc tagctcaatg gcctcttaag ccactaaccc aatatgttgc 3480tttttaccag taaaaaagtg catctgggga ataagttggt gtatatttgt ggttcagcca 3540gcccatagat cgtccagagg accccaggct taggaggaat cctcagattt gagagccact 3600gtcctgtatg atgcagatag cacagctctt cctgaaaata ggaattcaaa tgaggaaaaa 3660tgaaaactct cctaattatt ttcttctgcc tctttcattc caagctcatc taattatcat 3720gctctattgt gaagaatcaa agaaagaact cataaatata ccttttgtgc ctgtattttg 3780aatgtacgtt gtgcatgtaa gcctttgtgg ttaagcaaat gtcaagaaaa tccctagtcc 3840cattgcttac agtcaatatg gggcaattca agcaaagtat atgcctctaa tatggtttga 3900aaattctgaa agttcagaaa aaatgttgct acttcagcca tctgatgtta ctggatattg 3960agaatgagac ctcctctccc tctgctttcc tcatttccat ctcctccttc ctctttctcc 4020tctctccctc tcctttattc tccttctccc tcctctctct ccatgcttac tcccttcctc 4080ttctccctct ttcttcttta ttctccttct ccctcctctc tctctctccc ctttttcctc 4140ccttcctctc ctccctccct ctcctttatt ctccttctcc ctcctctctc tctctccccc 4200ttttcctata ttcccagatt aggccatttt caaaactggt taaacattcc ccaaaagcat 4260ttatgacaga gaattgacta ggatctcacc tgaatcacag agcttgccca ttgaacacag 4320gcaaagatca agccattttc tcttgatgtg atgcttcagc attttatgca tttgaaacaa 4380attttatgta aaaaaaagct tagccctttg gtgacgggga ttccttttct ggatgatctg 4440ccaaagaatc aagacccaac gtccagctta tcaaattttc tctggacctt tannnnnnnn 4500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgtggtg aagacttcca cgtgaacttt 4620aaagtagttt tttccaattc tgtgaagaat gtcattggta gcttgatggg gatggcactg 4680aagattttct tcatttttat gactgcatag cattccatgg agtatatgta ccacattttc 4740tttatccaat tcactgttgg tgggcaccta ggtcaattcc atgactttgc tgttgtgaat 4800agtactgcaa tgaacatgtg agtgcatgtg tctttttgga ggaataattt gttctctttt 4860ggatatatac ccagtaatgg ggttgctggg ccaaatggta gttctgtttt aagttctttg 4920agaaatctcc aaactgcttt ccacagaggc tgaactaatt gacattccca ctgccatgca 4980tgagcattcc cttttctcct cagctccacc aacatctgtt gctttctgac tttttgataa 5040tagccattct gatgtgtgag atggtagccc attggggttt cttttttttt ttttaagaca 5100gagtctcact ctgtcaccta ggctgtggca caatgttggc tcgctgcaac ctctgcctcc 5160tgggttcaag tgattctcct acctcagcct actgagaagc tagggttaca ggcacctgcc 5220accacaccca gctaattttg gtatttttgg tagagacagg gtttagccat attggcaagg 5280ctggtctcta actcctgacc tcaggtgacc cacctgcctc ggcttcccaa agtgctggga 5340ttgcatgcgt tagctaccat gggtggtttt tgacttgcat ttctctgatg attagtaatg 5400atgatccttt tttcatacat ttgttggcca cttgtatgtc ttcttttgag aagtgtctgt 5460tcatatcttt tgcccatttt tcaatggggt tgttttttcc ttgtccagtt gtttaagtta 5520tttatagatt ctggatatta gtcctttgtt gggtgcataa tttgcaaata ttttatctca 5580ttctgtaggt tatctattta atctgttgat agtgtctttt cttgtacaga agttgttcag 5640tttaattagg tctcacttgt caatttctgt ttttgttgta attgcttttg aggacttaat 5700cataaattct ttcccaaggc ccatgtccag aatggtgttt ctgagatttt cttctaggat 5760tcttttactt caaggtctta tatttaaatc tttaatccat ctggagttaa tttttgtatg 5820tggcaaaagt taggtgtctt gttttatttt tctgcatatg gctagtcagc tatcccagga 5880ccattcattc actagggagt cctttcccca ttgtttactt ttggcaactt tgtcaaagat 5940tagatggctg taagtgtgca gatttatttc tcagctctct attctgttca actggtgtag 6000gtatctgttt tcgtactagc accatgctgt tttggttact gtagtcttat tagtatagtt 6060tgaagccagg taatgtgatg cctctggctt tgttcttttt gcttaggatt gctttttggg 6120ctctctgttg ttgttgttcc atatgaattt taggacactt ttttccaatt ctataaaaaa 6180atgacattgg tagtttggta ataatagcat tgaatctgta gatagattgc tttgggcagt 6240atggccattt taatgatatt gattcttcca atccctgagc atggaatgtt tttccattta 6300tttgtgtcct ctctgatttc ttttagcagc gttttatagt tctccttgta gagatctttc 6360acctccttgg ttagctgttt tcctaggtat tttaattttt tgggcctttg taagtgggat 6420tgcaagcttg aattggttct tagcctgaat gttattggtt tatagaaata ctactaattt 6480ttgccatcca ttttgtatcc tgaaacgtcc ctgagtcatt tgttaattcc cagaggcttt 6540gtggagtctt taggttttct atttttagaa tctattcttc ccaaagagaa atactttccc 6600ctctcttttc ctaatcggac ccctttattt attcctttgc ccaaggcttg gaaagccctt 6660ccatactttt ttaaaaagaa cgtggaaaaa ggcccctttc ttattctcgt ttaaaggaac 6720cttccatttt cgctgtcaaa tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6840nnntttgttg ggaatatccg gtgcttattc ctcttcagga gttttattct tccaaaaatg 6900cagcttttca ttcctcctcg tttttctttc tgtgccaaat gcctccagcc tttccatgtt 6960ggtgcggtaa gctagacatt tttagtttgt agtgattgtt ttcgtttgtg tttgtatgtt 7020tttgtgccca tagtcacttc tgtaatgttc gtttctgagt ttcctttggg gggattaacc 7080tgccactgaa gtgtcagctt ccctgtcatg ggactgtcct tggaatgaca ggttttcctt 7140ccaccatagg atctgccact gtgaaggaga tgatgagagc cccctgatca ccccctgcca 7200ctgcacagga agcctccact tcgtgcacca ggcctgcctg cagcagtgga tcaagagctc 7260cgacacgcgc tgctgcgagc tctgcaagta tgagttcatc atggagacca agctgaagcc 7320actgagaaaa gtacgtggga ggaagggatg cttcaccctt cctgaaacca gtgaccccca 7380gaaagctggt gtctcagatc atgaggtcat ggcttgcctg cgttcatttc cttaaaaagc 7440tggggctggt gcttacattt gcattgtaga gattgttgtt tctggtgctg attttcaagt 7500ccctcttctc aggaagaagc tctttctgac cattcataat ccttcttttc ccttcaaagt 7560aagactgctt gcttgtcggt gtcagtcctc tggcctttga ctacagactg tttgtgacat 7620atcttagcac ctaattttat acatattaac tgttgacgtc ctgcttctct gattaggact 7680gtctgttgga gattagtatc tccccagcac ccagcacagt gtagcataca gtaaatttat 7740ttgtatacac ccatgataac tatttgctgg atgggagtgt gagtaggtgg atagggaaaa 7800gaaaggaagc acaaacgttg cgtgatgtgc aactgtcttc agaaaggacc cacaggcatt 7860gcatgacacg cagctgtctg gaggcccatg tcactgctcc ctctgagagg agctttgtcc 7920gccacactgg cttcctgttt aaattttgac tgtttcatat atctctctag atgcccagca 7980caaaaggttt tgtctggaag aataaaaaac tgttcctatc tcagaacatt aatatataaa 8040ttgcaagata agtcacataa tcaaatacta gccaattaaa tgctaaatgg tgtgctggta 8100gagcataaca gtcattcaga aaaggaaatg ctcattatga gctagagtgc tggggaagtg 8160gagcgatggt agcctcatag ggtgtttggg ttttagagca gttcttgaag gatcactagg 8220atttggaggc agagtgcaag gagtttagag aggtcaggct gtctgtggag taaagcatac 8280cctagaaagg tgaaggtgaa cattgacagg gtgtcaagct acgtgcgttg taggaagtag 8340cagggagcat ggggcactga ggcactgact ctgtattata ggtaggaaga ccagggaact 8400tgctagtgta taaaatccca gcagaaaaca accagattta gagaaggctt gtgcaaactg 8460cttaaaaaaa tgttttgccc agtagaaacc ttgtaattta ttgggtgcct actctatgct 8520aatctctcaa catgggaagt ccttgaggct ctattttcaa ctaccctgca tagactcatc 8580tggaaagagg ctgtgactag ggttggcaaa ggatatgtgc agagtgttta cacattgttc 8640tttctctcta cctttatgtg ttttctcctg attcggaaac cagtaaatga aaaagaaagt 8700ctggctattt ggagtaaatt aatgagctcc tagaggagat gggactagca gagtctgctt 8760gtaccaggaa ctcttagcgt cgatttcgag ctgttgctgc caaagtagca aggaccaaag 8820agtgagcgtc ttctgccatt caccttcaca atgtagtgtg acccatctgc tagtgtgttg 8880tggcatcatc aaccctcttc ttccgctcgt ccatgccagt cagcacatca gggagactta 8940aaacgtagta cctgggtcct gggccaacat actgttttca ttaggactga aatcatagaa 9000ttctttatca ggccatctta tgtctgcttg caagaataag cagacaaggg gatttttgat 9060ggtagttctg cattcatctt gatcctggct tcttttcatt gcatagaaag ttggctgcac 9120ctggtacctg gttgaagcca agtggtttta ggagaacaat acttggggga atgcatctag 9180tttgggaagg gagaagtggg gtggaaagcc aatcttacct acctacaatg ctctcacccc 9240accggagccc ttccccagaa gcctgagcct cacactctgt gttgtctttc tagtgggaga 9300agttgcagat gacgtccagc gagcgcagga agatcatgtg ctcagtgaca ttccacgtca 9360ttgccatcac atgtgtggtc tggtccttgt atgtgctcat tgaccgtact gctgaggaga 9420tcaagcaggg gcaggcaaca ggtgagttct caggctggag caagctcagc atgggggccg 9480ggatagctca caggagtcct ggggtcagag gattttccag ttagccaaaa acgtctgggt 9540ttccggagga gggacatcag ttaggaggca ctagcctcct ttttggcatt tcttttgctg 9600tggtggtagt ttttgaaacc cagtcaggag agttcatatt ctggagcatg agaatctccg 9660caaactacat cctataaaag caggaagtag ctggacactg tcttccactc ttctttgact 9720gagcaacatt ggatgagttg tgaatccatt ggtcagtggt gcaggcttgt ggtccccaga 9780tacttgaggc tgagggatgg aggattgctg aggctcagga gttcaaggct gtagtgtgcc 9840atgatcacac ctgtgaatag ccactgcact ccagcgtggg caacatagca agactccatc 9900tcttaaaaaa caacaacaac aacaaaggtc ttcaccaggg atctgcctag gtcagcgagg 9960tggttcatca agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggagtgt 10080acttcacccc gtggtgaaga tgcggcacag actggcgttt cctacggcgc cttcactgga 10140aagcgcccct gtggtcctgg ggagagggcg gctccatccg ccacccttcc cggagctgcc 10200cccgcccatt ctcatgggag gaaatcgcag ggcgtgtgag cccccgaccc ctcgccccca 10260cctctgccag gaccggccgc atttctgcac tgtcccgccc cactgggaag gaaactctct 10320ttctcaattt cctttaatga ggacattgtc ttttcagccc tgccaccccc tgcaatcgtc 10380ttccaggctg caaacagcga gtggggaggt gaacacatca gcttttcctc cctccacacc 10440accaggaaat ttcgcaaacc acttggctct cggcggcctg gcctcgccac acctgtgccc 10500acagtctctc tccgctgcag ccccctgtgc cattctccag gccccacgta cctctgcctg 10560ggcccagtaa gacctggtca gatggccccc ggctgcaaag ccttcctggg ccccatcaga 10620gaggacgccc aggtgaagcg agggtcccct gcccaccctg caagcgcagg ggcccttcca 10680agagtgcagc cctaggccgg tgtgtgatgg tggagacgcg gtcccagagg gcctgaacca 10740agaatggaga agacaaaggc tgtctttagg gagaaacttc cctgaggggc tgatgtgtgc 10800gatcccattt taatctcaca accctgggga ggagctgggg ccgggtctca ttgttcattt 10860gaggacaccg aggctcaggg aacttagacg actttcctag ggtcccccca acccccaccg 10920gcccagggcc ctctcccagg cacccctcat ccccacacag gctcctctaa gctgcttcct 10980tgtggtccag cccccttggc tcagctcctc aaatacaggg acacctcttc tctcccccgc 11040agaactcaag ggtctccatc cactgtgcca gcacagaggc ggtgctcaga gtggggctaa 11100gcttactgtc cagcctgctg ggccaggagg ggcagaaggg cccctctcag gattccttcg 11160ctctgctcct gccaataggc catcactcaa ggggctaatg acaggacgat gtggagaaac 11220gtgggctgca gcacttctgg ggccaagggc agggtgggcg gcactgggga gtacagggga 11280ggggacctca ctggggccag aggcttctag aagcttctgc aagaaggggc ccctcaccca 11340tgtcttggcc aagaagtggg gagggtgggt gtggcaaagg aaacagctag gtcagagaca 11400agagatgctc tgcaaagccc tgctggctcc ccacggtgga gcatcctatg catgtaggac 11460ctgagagatg gcaggggcgg gtgcgtccct atggatgtat ctccatttct gggcagagac 11520atctgggctc tcacctgcaa ttgagccaac caagaaatta tatttacttt ttagagcgag 11580cctccaacaa attatacaag cacttgagaa taagccctcc cactgtcttg agagatggag 11640cccagggagg cagctggaaa gatgctcctg ccctcactca ggagagatgg tggagctggg 11700agaggaggag gcactccctg ggacccagca gatggacttg actcagcaaa tagatgcaac 11760catatgtggg gggtggggag gcccagaaag gcattacagg ccagtgttct taatcctggc 11820tatacactgg aatccaccag tagccctaaa atataactgt gctggacttc ccctggttcc 11880acagtttgtg cagagtgggc agcatggagt ctgtaacact aaacacacag gattgagaac 11940catatgtcta ggctgttgtt agccatgatg gggatacatg aaaaggaggt gcacagttac 12000cttctatacc acaggatagg tgctcattaa accctgttaa aatggactgt gacccagctg 12060gcactcaggg gcactcaggg gccaggtggg tcacactttc attttaatag gatttaatga 12120gcacctattc tgtgacagct gctaagatac agaggctcct gagatacaga gatgacaatg 12180accttcagaa acactgaaca gagataagaa aggcacagtc tggccttcac tccaccatta 12240attcctccat ccatccatcc atccatctat ccatccatct ctctgtctat acttccatcc 12300atccaccatc cattcaccat ccacccatca atccatccac ccatctatcc atccatccat 12360ctcactatct gtcatccatc catccatcca ccatccatga atccatccat ccgtatatca 12420atttctcatc tatctctcca tccatccccc atccacctct tcttccatcc ttcctccttc 12480ccttcatcca tccattcatc atccatccct caacccatcc atctgcctgt ccatcggttt 12540gattgtccat ctgtccagta ttcctccatt ccctccaaac tgggtggcag tactctccac 12600ctcactacct caccatcctt attattccct ccaaactgtc tctttcacaa gggccttctg 12660tcctgcctgt cccatcctgg tctgggacct atcccactaa agctacatgg ctttgatcaa 12720caatcccaat cccagagtag gggaagggaa gtgagcccct aactgccgtg atgtttccag 12780ggagatggtg gatactgctg cattactaat ccagatgtta ggttttaatt tttctctcca 12840gtagacccca attgtggtag gagaaaaatg ccccagcttt ggacacaaac agacctggat 12900tcaaatcctg gttttgccaa tttctgccta aatgacttta ggcaaactcc ttgaccttga 12960ccccttggag actcagtttc ctcacctaga aatggtcaat aatattattt acctcccagg 13020atggccatga agcttaaaat agattattgt accccccaaa acaccgacac aatgtttaga 13080aataaataat attattttaa gctttgaagt ctggctgggt tctcagcctc gtctacctgc 13140tgggtgtgca gactcaaagc cagggtccca ccctcataga ctgtcatctg tgggtgtggg 13200gttggcctgg cactagcagg tgctaatgct cctcagagga ttctaggtgt aaaaggaaat 13260gaaaatcttt ggaccccaat tcactctgcc aaaagggaaa aattaagctg aaagctgagt 13320gatgcaagaa acgaagatag ctacagataa aaggtgaaaa cctccacagg tagctcttgg 13380tgttcacctt atcaagtgct gatgtactaa gcgcaagagg aatacataac tgactattcc 13440cctacctgct ccttttcttt tgcaacatgt ggattcagta acgtgaccat gccctccctt 13500tcttccttcc agcctgttct cctttaaata ttgatgccct taaagacatc ttcggaaaaa 13560ggcacagtcc accaactgtt cctgtggatt tgtgttcctt ttttccgtgt atgtcttttt 13620ttttattatt atactttaaa ttctaggata catgtgcaca acgtgcagga ttgttacata 13680tgtatacatg tgccatgttg gtgtgctgca cccattaact cctaattagg tatatctcct 13740aatgctatcc ctcccccctc tccccacccc atgacaggcc ctggtgtgtg atgttcccct 13800tcctgtgtcc aagtgttctc attgttcaat tcccacctat gagtgagaac atgcggtgtt 13860tggttttttg tccttgcgat agtttgctga gaataatggt ttccagcttc atccatgtcc 13920ctgcaaagga cattaactca tcctttttta tggctgcata gtattccata gtgcatatgt 13980gccacatttt cttaatccag tctatcggtg atggacattt gggttggttc caagtctttg 14040ctattgtgaa tagtgccaca ataaacatac gtgtgcatgt gtctttatag cagcatgatt 14100tataatcctt tgggtatata cccgataatg ggatggctgg gtcaaatggt atttctagtt 14160ctagatcctt gaggaatcgt cacactgtct tccacaatgg ttgaactagt ttacagtccc 14220accaacagtg taaaagtgtt cctacttctc cacatcctct ccagcacctg ttgtttcctg 14280actttttaat gattgccatt ctaactggtg taagatggta tctcattgtg gttttgattt 14340gcatttctct gatggccagt gatgatgagc atttcttcat gtgtctattg gctgcatgaa 14400tgtcttcttt tgagaagtgt ctgctcatat cctttgccca ctttttgatg gggttgtttt 14460tttcttgtaa gtttgtttga gttgtttgta gattctggat atcagccttt tgtcagctga 14520gtagattgca aaattttttt cccattctgt aggttgcctg ttcactctga tggtagtttc 14580ttttgctgtg cagaagctct ttagtttaat tagatcccat ttgtccattt tggcttttgt 14640tgccattgct tttggtgttt tagacatgaa gtccttgccc atgcctatgt cctgaatggt 14700attgcctagg ttttcttcta gggtttttat ggttttaggt ctaacattta agtatttaat 14760ccatcttgaa ttaatttttg tataaggtgt aaggaaggga tccagcttca gctttctaca 14820tatggctagc cagtcttcac ccggggctga aggtaccgct tnnnnnnnnn nnnnnnnnnn 14880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 14940nnnnnnnnnn nnnnnnnnnn nacaatgaga acacatggac acgggttggg ggggtatcac 15000acactggggc ctgttgggct atggggggct ggggagggat agcattaggg gaaataccta 15060atgtagatga caggttgatg ggtgcaacaa accaccatgg cacatgtata tctatgtaac 15120aaacctgcac attctgcaca tgtaccccag aatttaaagt ataataagaa aaataaaaat 15180aaataagtta aaagagatta aaaaaataaa agagaacatt tgccacattc agtctttcta 15240gatggaaaga ggttgctgac atatgataga attagaaaat cacacatctt gtaaattctc 15300atttgtttaa aaagaaatca tacaaattag atgttttttg gagatgactt tttaaaatag 15360agtcgttaga tcacctctgt aagggatatg tctatatctg ttcagtgggt taggggacat 15420ggatctggaa agcctgagaa gaaaaagaag gttctatacc agacttgtga tatttagaca 15480ttttcatatt ctatccattg ttttgtgtgc attttattcc tcactattgt atatatagtt 15540gacaatgcta aacttttttg tgtattcttt ctatgtgttc cgaatgccta atatatgtca 15600aaattagcgg tagtaaaata atattttgta aatatctttt tgctaaaatt catatgaaat 15660gttgtttttg gaggggaatg gccaaactac ctgttgagta atactcatcg tgtttgtgtc 15720ctggttcagg ggaggaggaa ggaggggaaa gtgcagagag ctctatgccg ctgtgtttac 15780agtgaggcaa gattaaccat tatctcttat gtctgtgcat tttgttttac ttatctgttt 15840atgtagtgta tataaaggac aaacgagtcc taatttacaa catctagtct ttttagatgt 15900taaagaggtt gccagtgtat aacaaaagta gagttagtaa actaatatat tttgtatatt 15960ttgttttaaa attcctagga aagattgtct tcctatcttt gagcattctt gcgcactgag 16020ttgatggaga tgggagggat tctaagctag gatgttctta tttggaagac tctttcaaat 16080tataactatg gttacatgta tgcagtttat tcgagactgc tgtgtatata gtggacaaat 16140taactcctta cttgaaacac ctagtttatc tagatgttta gaagtgcctg atgtacgtta 16200aatgtagagg taataaaata ccactttgta aatatctttt tgctaaaatt cataggaaat 16260actgtctttt agaaatttaa ttgttaagcc acctttgtga gcagtatagt actgtctata 16320cttgttcaat ggtttagagg aggtgggagg gaagaaaaat atgtgactga cttatttcac 16380taagcctaat gtctttaagt ttcattcatg ttgtggcatg tgtcagaatt tccttccttt 16440ttaaagctga ataatattgc attgtataga tatattacct tttatttatc catttatcca 16500tcaatagaaa cttgggttgc ttccatattt tagctatcat gaatattgct gccatcaaca 16560tgttgagttc ctgtattcaa ctctttgggt aatacccaga agtgaaattg ctgaataagt 16620atggtaattc tctgttaaat ttttgaggag ctgctgattc atacttttac gtataaaata 16680ttagacctta aatgtattat gtaaaaacac taagtatttt taacattcta gagtaaagaa 16740ggtgtttctt agcaagacag aaaagctgaa accctcaata gaaaagcctt gttaatgtta 16800tcacataaaa tattcaaaat ttctgaacac acagacacac acgcactaac gaaaagctat 16860ttacaacttg tggtagatga agaaataatt tcttaacata gcaagaaccc aggcaaatga 16920ttaagaaaaa gaataatcat acaaatttaa tacatgtgag aagctgttca gcctttccca 16980ttaattaaag aagtaaaata taaaacaaaa catataattt ttcacttagc agagatgaaa 17040taccaaaaca gatgggtata ggtgtggaac atacttcctt ggttgacagt tagccaatat 17100ctattaaaat cccttgccac agtaatttca gttctagaca tttgtcttat attactgtat 17160ttattctgtc ctgtaaccat aaattccaca gctgcctagt gatggttcta tatttaaatg 17220gaatgcttac tctcagtatt gttttttctt ttgagatgga gtcttgttct gttgccaggc 17280tggagtgcag tgccatgatc tcagctcact gcaacctcca cctcccaggt tcaagtgatt 17340ctcctgcctc agcctcccga gtagctagga ctacaggtgt gcaccaccac gcccagctaa 17400tttttgtatt tttagtagag atggagtttc accttgttgg ccagtatggt ctcgatctct 17460tgacctcatg atctgcccac cttggcctcc caaagtgctg tgattacagg tgtgagccac 17520gacacccagc cagtattttt ttttttaaac cacagcttct tcattttgag tcatctctaa 17580atagctgcaa tttactataa cttagggttt acaattttta aaattgtgta tgttaaggta 17640tccacatgat gtttgatata cttcttttga cataaatata cacaatgaag tgattactac 17700agccaaataa tgattaccat acccatcatt gctagcgttt agaaagacaa ctgattttta 17760tgtgctgata ttgtaccctg caaccttact gaatttacta aatctagtag ttttttggtg 17820catcctttgg gattttctat taacagaatc atgccttctg caaatgtaag taatttagct 17880gcttcttttt aaatatgtat gcctcttatg tctttttctt ggctatgttc ttttctctgg 17940ctagaacttc tagtacaatg tccagtgtca gtgttgaaaa tgggcatcct tgtgttgttc 18000ctgattttag gagtaaagct tacagttttt ctacccctga gtatgatttt agttgtgaga 18060ttttcctata tgccctttat catgctaaga aagttccctt ttattttagt ttgctgattt 18120tttaaaatta tgaaagggca tttaatttta tcaaatactt ttttctgtgt ctgttgagag 18180tttggccatt ttttttctat tgaaatggtg tattatggta gctgattttt ggatgtgaaa 18240ccaaccttgt gtccttggga taaatcccat gtaatcatgg tgtataattc tttctatgta 18300ttgctggatt tggtttgcta gtattttgtt gggaactttt gtgcccataa gataagaggt 18360attgatctat ggttttcttt tcctgtgatg tctttatttg gtttttgtgt cagggaaata 18420gtggcttcat aaaatgttca agaagtgtac cctcctcatc tttttgttgg aagagtgtga 18480aaaattggta ttatcaaatg tttagtagaa ttcaccagtg aagccatttg cttttctttt 18540tgggtagttt ttgattactg attcattctc ttaatttgtt ttaggtctat tcagattgtc 18600tgtttcttct tgagtcagtc ttggtagttg tgtctttctt gaaatttttt catttcactt 18660aaattatctt atttgtcagt gtacaattat ttttggtatt cctttataat cctttttatt 18720tctttaatgt cagtagtaat gtctcttctt ttatttctga ttctatagtt tgagttttct 18780ctcctctttc cctgatgaaa ctgaagtttt ttccatttta ttgatttttt tcaaacgatc 18840aaattatttc attaatttgt gctgttatca ttatttcctt ccttctgctt acttaaattt 18900agtttcatat tttttgtaag tgtcttaagg tagaatttta agttattgat ttaagaactt 18960tcttaataca agtgtttaca gctgttgata ttactctaat tacttcttta gctgcatcac 19020aattgttttg gtatgttgtc ttcattatta ttcgtctaaa aatattatct aatttctgtc 19080ttgatttctt ctttgactca tgaattgttt tagagtggaa atttcatttt aaatttccaa 19140atatttgtga atttcccaaa tttcccctgt tgttgatttc taattttatt ccattgtggt 19200taataacata ctttgcatta tttctatcct tcaaatttat tgaggtgtgt tttatggcct 19260aatatgtggt ctatcttcta gaatgttcca tgctcacttg acatgaatgt atactttgtt 19320gttgttgtta agtagaatgt tccatagtcc ttgaggtcta tttggtttgt agtattgctc 19380aagtcctctt tttccttgtt gatccgtcca gttgttctat ccatttttga acgtgtagta 19440ttgaagtcct tgactatttc tgttgactcg gttgacttgt ctatttcttt cttttctcag 19500gtctgttttt gtttcattta ttttggtgct tcatatatat aggtgcatat atgttgataa 19560cagttatatc ttcctaatgg atggattcct ttgtaattac aaaaggccac tctatctcta 19620gtaacaattt ttgttttaaa gtctatttgt ctgatatcag tgtactgact ccagtgtttt 19680tgtggttgct gtttgcataa tatatttttc ctgttctttt actttcattc tgtgtcttcg 19740aatcaaaatt gtgttttctc cagacaatat atacttcatg ttttcttatc caatataaca 19800cctttgcctt ttgattgcat tgtttattcc attcacactg catattatta tttagtggga 19860tttatgtctg tcattttagt tcttttttgt ttttgttttg agacagaatc ttgctctgtc 19920acccaggccg gagtgcagtg gcgtgatctc ggctcactgc aagctccaac tcccgggttc 19980aggccattct cttgcctcag cctcccaagt agctgggact acaggtgccc accaccactc 20040gtggctaatt ttttgtattt ttagtggaga ctgggtttca ccgtgttagc caagatggtc 20100tcgatctcct gacctcgtga tctgcccacc tcggcctccc aaagtgttgg gattacaggc 20160atgagccacc gcacgcccgg attttttgtt ttttatatgt atcatgtctt tgttgttcct 20220gttttgtctt tacttctttc tatttcattg aattaatatt ttctaatata gtatttaagt 20280tttattaata attttatcac tgtattttta tttttagtgg tttctctagg attttctgta 20340tatatcttat cagtatgagt ttcagattca cactagctta attccagtaa tatgtagaaa 20400tgttactcat gtagaactta attctcttgc tcttttgtgg ttattttatt tatattatat 20460caattaatgt tacaaagata tacattgttt aaattattat tggtgctatt attagcatac 20520ataacaccca catattaaaa ttgtatatat tgcatatatg tattatagaa ttgtatatat 20580tatagggcca atagtatatt gtatacatgt tattttatat aattgttttt aaaatgagtt 20640aagaaaaaag aaaaatgcat ttctactgtc tattatagaa aaataattac atcatactga 20700acgtcttcac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 20760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctctaata 20820aatttgcggc cgctaatacg actcactata gggagaggat ccgcggaatt cctaaaagaa 20880ttgctgagac aaagtatatg tgcctttgtc atcctgaggg cagttgtcat cctgtggagg 20940tttaccattt gctctcccac cagcaatgta taagcatacc tgcttcccaa cacttccgcc 21000aacacatatt atttgattaa ttttcttggc tgataatggt atctcattgt agttttaatt 21060tgtatttctc attgtgagag aaatgaagca tcttttcatg ttttgagagc catctgtgca 21120tcttttccca ttgtttctgt taagtcattg atttgcagta ctgtaatttg tgatgagttg 21180taaacatgag ctcttctggc ctcttaacca tccttatcca gcctgtcttc acgttcctcc 21240ttgcctaaaa acaagctcac tcaaacagat cattcctgtg gccctctccc ttttgaaata 21300aatcagatgg gcaaatttgc ttttgttagg ggcctgagac ccaaatgttt aaaagtagct 21360tagctgatga gtgaaaagga ggcccagttg ggggttgcac agaaaatgag gggcgtgggt 21420aatagaggag gctgggcagt aacctggcag aaggcgccag aggccaagga aggtgcgctg 21480gtgtcctgca gtgagaagga tgtgtgcact atcaactggg taaacacact gctatagctg 21540ctccagcgac agcaggcact ctcagaaggg aactgagaaa aggggaatgc cagtttctgc 21600caaggatata tggaaaagtt ttttttcccc gtatgttctt tggcaacctt gttaaaatca 21660tttaaaatgg tgaatttgat aacaaagcca gccactaaaa gacttggatg tagcaaaaca 21720aattgtcaaa aagtatttat catttctaag aaataaaata aactgggatt taggcttgga 21780tagaaatcca gaggtttgtt ttggcataat taattacaac atctgaagaa gggaggattt 21840ttacaagaat tttttttttt gtagtgagat tgctttatat tccttttcat gactggagag 21900tctgtcattt ggcaacccgc aaagaacttc aggaacagag ggcctaagtt ttcttgacac 21960atttggattt ttgcatgatg aacatttgca cttttattag taattgtcga gtcgtgtgtg 22020gtctgatcct atggagagga gagtgtaggt gtnntctctg gagctagtac ntgcccccgn 22080gctcacacgt gccacactgc ctgctgcttt cangaaagag caggacggct ggtcctgtcc 22140catctagtga ggtcctgtcc catctagtaa ggttctgtcc tcagagctcc taagagggga 22200ttgctggcct ttgattacac tggcttccag aatctgacca tggaaccatt atttgaagct 22260gtcagcttca ttttgttgtt gatcaggcca gtcttttagg tctttgttgg ttgatagatt 22320tctctcaggt ctatagcagc aacaaataaa gctctttaaa attattttct tgaccattct 22380gcctatggat tggttctgac ctttgaaatg tttgctgtcc tttagatcgt gacagaagga 22440gcctcttctg ggagctgtga agaccccaca gatctcactg gttttcttgc ttttattgga 22500agagttagca tttgttgaag tttatattta tgtaaaataa caataagtag attttaaaat 22560ttttctttaa attctgtgat ctaataagaa gagagactct ttctctgtca ttgggaggct 22620tgatgaggat cggacatacc tgctgagagt atcctgtaat gttttcataa gattggaaat 22680ggtgaaatga gagcagaaat gtaggcagag gatgccaacg ctgtttccta tttcctaagt 22740aaaactgagc agggcagaat ctccccagcc ctgccagaac tcccttcctc cttgggtccg 22800ctgtcactgt catgctggcc ttgacatctg ctacaagtgc acacacatgg ggaaccacac 22860agggaagtga acacatgtcc tgacgtgtgt gtgtgcctgg tgtggctgct tccttgatcc 22920aggctttgtt ttgttacgag gctctgccct cctaccttca ggccttgctc ctcatcatgg 22980gcatgcaagg atggaatcgt taccccagag tacacttttt gacttggatt acttgtagtt 23040tagtactgga gttcagaact ttaatttccc atcagaaacc tgcctcccgg acccaggaca 23100ctgaccttga ttgggcacga ttttccagat aagaccttac ctcctcccaa cagggtttgc 23160cttcccttca gaatacagga aagtacacgt ggttccaaga ggaccatgct cttactaaga 23220taaaagggag gagggtgact aggtactttc ctctgctgcc atcccttgaa ggaagggggc 23280ttccttcaat gcctcatcag ttccagggag tatgcttgac ccagcaacac agaggaagaa 23340gtgacctcat agcaatgagg ggcaagcatg tggatattgc tgggtatttc ttcctcaaag 23400tctacctggg gacaggtgat gaagacgctt acttctctgc aatacaccgt gatcaggctt 23460tggtcttcat gacacatttt agccttgcct atgtgtgttg aacagagctg gtcatcctca 23520gaaataaaag aagagagcaa taagtacagg aaggctttca gtcccagaat agtgttagga 23580ctaaatgtca gagaacaggg ggactgtagg agtttgggga ccttagcatg gataccctct 23640caatctgctc ccctgtggta taaacagtaa agcccattgt actaaaaggc cttgagtgct 23700tgtctctggg gcaggtggag ccagtgatgg gtgccccgag aggaggatgc tggagagggg 23760acagtgtcta aggcagttgg agaaaaagga caatggctta tgcagtcagg cctagaagat 23820gcaagggatg tcacagcata gtgtccacag gcatccctag atgtctttca ttttgtttca 23880ggaagaatat tgacaaaggt agtgatggga gggttatttc aaggatgcta ttttcatgtg 23940tccaagtaaa cttaagattc ctaagaactt tgacctctaa tgcttttaaa gaccgtgtac 24000ctcatgccca tatgcacatt cagttagaaa ataaatgtag attctttggt gaattcgaag 24060atgtgctcat ggagtggcac agcgtgatct cagtgtacac atgaagcatg ctgatgagag 24120cttcttggat cttgtttctt caggtggact tgcattaaag tgctgttgaa tctctgcact 24180gggagtggga gcagccatgc gatgggtgcc ttaatccgag tgccatcctg gctctctcca 24240ggctgcttca tttgctgtga ggggtagggg gaggaggagt ttccaggcaa tggccttatc 24300tgttctggtg gttgaccaag ggtcgggggt cccaaaggtg gagttccagg atgagagtgc 24360acccttgggg atggtactta gaaagagtgg ggactaggcc tcagccaggg aatggtgggc 24420cagggtggag ctcctttgta tcaaggagtt agggccaagg gccatgcagg tgggcctgca 24480gaaggtgggt ggtgatatgg cctttctggt gataacagac tcgtggcact gttttcctgg 24540ggctgttggg gttgcatgga gcagattctt actcaggctt actgtcttaa ggagggaaga 24600atgctttttt atgaatacgc ataggtctgt ggggctgtgg agcctgggga atccgaggct 24660ggcgtgggag tctggggccc tctagcggtg gtggtgcatc ctcagctcag tgccactcac 24720tgtccctatt tctctctgtc catttctctc ctcactgcag tgcttcctct ccttcctaga 24780tttttttctc taagtttgac tattcaccac cccagagact ctgttctatc ccatgacctc 24840ttgctgtact ttctttcaaa tctaaatttt cttgattatt tttcttaatt tacagtttca 24900agattagatc tgttgggagc attctgtgct ctacctcagc ccctggttga gctatggatt 24960ggtgtccccc atggtgacgt ggtctggact cccttttgtg tgaagagctg tggcagggca 25020gtgaggtttt gtggaactca gggagcatct ggcactatgt atgaggttgt gaggtgggag 25080agagctctta aatctaccag ccttgttgat cttgggaact aggagagagt gctagggaag 25140ccagtgccag caggcctgga gcctcatatt tctttggcat ttgtatgata ccttctagta 25200cttcgttttt tgacttatat atgaactaac tcaatttaga tgcttagaaa ataaacggcc 25260actggacctg caagagatta gtagtggtgt tccatagcct tagacatttc ttacactttt 25320atccacagat aagaaaaaaa tagtgatatt tgatttgttt tgccaaggct tagaaggatc 25380taatatgtgt tactcgttga gcagcgggaa gcagcagaca tttctagggt ttctccctgt 25440caccattcag gagacagtgc acacaatccc cttggctctt acctgaacac tgaccacagg 25500tctgtcattg caggctggga gtcctccgtc agcatcagct ccggctccgg tgtcctcctt 25560ctctcgcact tctatcacgc catccagcca ggacatctgc aggtaacact ctgcttcaga 25620tgctgccatg atcagtgtgc agccagaaat aagcattagt gtctgtaggt tcttgaattt 25680ccatttgctt aatcaatctg cctttggtct aaacagttgg cgagttttat gtgtggtttg 25740ctcaccaagt tacccatgct agtctcattt cttcactatg atgtctattc tcttttaaaa 25800ttgtttttac tttctgtgct tttatcccac tgttctgcat gcagttccag tgcagtgttt 25860tctgagtgtt gtcaccacag ttccgtgcag tctgctgttg tctcgaaagc tcctcactgc 25920cagagttctc tgacacaagg gctcactgtg acagttatct gtaaggacac attacaggcg 25980tcaaagagaa attcctttgg ttcagaatgg gcccaggcct tgaagcctgc taagaatacc 26040aaagccagaa gaacactaaa gttctcaagg tccctcaatg atgtgggtga gaaggcgcag 26100gatacttcag aaagttttgc ctatgtggaa agaacttgtt ctgaagggaa attaatactc 26160cctcaagata cgtgtctcag aactaacagg tttcatcata aagaaaaaag aaccctgaac 26220cacaaacctc ttggcaattc caaacattct tgtgtttcat gcctttctgc cggtcgctca 26280actgcctcag aggtggaagc tggcaagggg ggcaggcccg gcctgctgct ggaagagaag 26340gcggatggtg aggccacgtc ccgaagccgg caactgctcc agtacctgtt ctcactctcg 26400cacggcttga gcgccagcag cctgcacagg ttccatgagc tggagagctg cgctgctcgc 26460ctgcacactg ccaagtcctc cagcgggctg gcagggagta tgggcttctg ctctgacgag 26520atgggagacg acgatgtctt tgaggacagc acatctgcaa aactgaagag tagggttctg 26580cgggcgcccc tctgctccac ggaaaaggac agcgacctgg attgtccttc tcccttctct 26640gaaaaattac cccccatatc tcccgtgtcc acgtcagggg atgtctgcag gttggtttgc 26700caggaagtgc cattctagcc atctctgctc gcatcaggaa cctcctcttg ctttagtatg 26760tgaagtttca ggaaaatgaa gtaataggaa gttgtaacca gttgtgggtt gatggttttc 26820tccagaagaa tggagtatca atatcgtttg aaaaacttaa tttttatgat gtgagtccca 26880agatagtttt acaaatgata aaagaggaag aagctggctg ggcgcagtgg ctcacgcctg 26940taatcccagc actttgggag gctgaggcgg gtggatcacc tgaggtcagg agtttgagac 27000cagcttggcc aacgtggcga aaccccgtct ctactaaaaa tacaaaaatt agccgggcgt 27060ggtggcgggc gcctgtaatc ccagctactc aggaggctga ggcaggagaa ttgcttgaac 27120ccgggaggcg gaggttgcag tgagccaaga ttgcgccatt gcactccagc ctgggcaaca 27180agagtgaaac tctgtctaaa aaaaaacaaa acaaaacaaa aaaaaaaaga ggaagaagct 27240acacatatta aaggccaagc tgactcttct aaactaaaaa taagtagact ttgcataaag 27300aaactggaag aaagaaacta gtatcttcag ttttccagcc tgatggtcca gattttaagc 27360attgttgcat ctcccagcct cttttttaac ccttgctccc tacctacccc caaaaggtgt 27420gtggggactg gctgttctcc agtagctcct tccttccttc tttttaactg aggtgtagcc 27480acctatgtcc catcctaact ctgtttcccc cctttatctt cccttgtgca gcatccttgt 27540ctggcctcca taattcactt tttgtcatga ctcttgtgcc ctcctcattc tgacctttct 27600ccagtctccg ctccttagtg ccagctgtca tcatttctaa catttggatc agagatcccc 27660tcactaaaac agcctgagag cctggctcct tccaggacga gtgccccaac ctgttctcta 27720gctgctggaa aagacccctc tgtttcttaa aagcctgtta agttccttca ggctagtagc 27780tacaccttgt tgcatacttc cttggaaata gagagtgctt tcaaattaat ttctcactta 27840ttgctattag tggtcccaag aaaaagagat gaaattgcag catcttgtaa tggtgagttt 27900tttaggtcta ggagagaggt actgtcatct ttgtttactt gctgagagac gacagaaatg 27960tgtagttgtg gggtatgaag gtaaagtcag tgattatagc taaaaagcaa acgtcattta 28020aggtcactgg tatttttgga tgttggaatg cagcgcttac catccataca agaacccgct 28080gggagggcca agaccatccc gtgcgcggcg ggaaccgcga catccgccac tcggatgccc 28140gctggagcgg gcggagaccc acgcgcgcgc caatgtcccg gcgcgcgggc tgctcaccca 28200gggcagcggc gtagtcgccg ggggcacaag cccgagccgc ccggggcggc gcgcggagaa 28260ggagcgcaga ccccgtgaac acgccggagg accgtccgcg cggcccgtcg cgccactgcc 28320tactgccagg ggcggcgggt gacgacaaaa ccctcggtgc gccgcgcctg gcagtccgcg 28380gcccccgccc ccccagcggc gcgcacccgg gggcgcgggc cgaacccggc aaggtgagag 28440ggccgtggcg cccgggcgct gaccactatc attctccgcc acacggacga aggcaggagc 28500accacgggcg gccggcgggc cgcccgctcg ggccaaagag cccgcccgcc agcggannnn 28560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagg ccgggcgagc gcaggcgggc 28680ggagagcaga ccacgcggaa gaggccgaga gagaaagcgg ggcacccggc cgggggggtc 28740atttacaagg gacgctgggc acgaaggagg ctggggcgaa gatgagtcca ggggttgggt 28800tgagagcgct gcgcagccgg gctgagaaga gaagctgacg agtgactccc gacaagggac 28860gggggagagg gaacctgggg gcaggcagag gcctaggggg tgggctctga gggcagccac 28920tcccaccggc cctcccccag ccccgcaggc cagccacatg ccccgtgctc cagacgaccc 28980agctacatgg atggcagcgt cctgaagggg gatgatcggt agggagtggg tgcggaggct 29040cacgcctgta atcccagtgt tcggggattc ccgagcggga ggatcgcttg agccggggag 29100gtggaagctg cagtgatctg cgccactgaa ctcccgcctg ggcggcagag tgagaccctg 29160tttcaaaagg caccaggggt gtagggaagg agcgatatcc cccaggcaag gaaggctgcg 29220gaggggccag gccaggagag aagggccctg agctcagtgg acgggcaggg tgcctgtgga 29280ggaagggagg gacgtagcag caggaggaag ggtgaggacc caggctgggg atcgggagaa 29340gtgatggcgg aggaaagaga ggccggaaga ggagggggcc tgctggcctg gcactggaaa 29400gcaaagcgtg ggtttgtata tgggaagaag accacatccc ctcttatcgt catcccaact 29460cagaaaccat ttggccccaa gtcaccaaag cactcatagg aaccactttt cctgtgacac 29520aagtaggatt ctcccacttt taacttccca aatgctaccc gaaagcctct gaaatccttg 29580gaaatttaaa aaataatccc cttctattaa ctgatgccta taaaacatat tgactttcat 29640taaattttgt tatgaaatca gggatgtgtc ccattgtaaa ataaatttgt ttggaaaatg 29700tagggtggta tcccatctaa aaatcagacg aggctagaaa atttcaattt tatttgctaa 29760ggtagcatca tgttgttaaa aatgcatcat aaaataagac caagagttaa tacaaagcat 29820gaagtcatgt ttccctcaaa aaaatgattt gttctgtagt caaattaatt tggaaaacat 29880tgcaaactct tcagcagtgg ttctcaaagt atagcttgcg tcagaatccc ctggggccct 29940tgctaaaatc tggatggcta cactccccgc ctagagtggg ttaaactcag tgcccactgg 30000caggaaactg aaacttggct gaagtcaccg aggaggcctt ccccgttaaa cctctggctg 30060gcgacagctt ggtttcctcg ccgctgcttc tgagtaattc tggcacgcag ttcctcctag 30120ccacctgctg tacctggaag ggggccagcc ctcctcagca ggctttgctc ctgccctgca 30180cagcacaact gtggcgtaaa aaatggaaaa gctggtagca gaaggaaaga gcagggagac 30240agaggttagg tgaacctcaa tggcctcatt aaagcaaatt gagaaagaga gtttccttcc 30300cagtgaggcg ggacagtgca gaaatgcggc ccgtcctggg agaagtaggg gcgaggggtg 30360gggggctcac cgggcacatg gccaacagct ggtgcccagc ctgggccaca gtttggcaca 30420ggagaaagct gaagatgcag gagaaagcta ggtcactgcc cctaggccag gtgatcaagg 30480tggctgttac ctgcctgtgt ggccttgaaa aagcctggtg ccctctctgg cttgcagggt 30540ccttcttttt acaaaagcgg gtcttcgaag ccctgaatat tccagttatc tgtaaggata 30600tagaaatatt ttgcctctag atgacatgta aatatgttcc tcctaactcc acttcttcct 30660acatctgtgg cttcttctga gaactaccag acccaccctg ttttcagagt aaacctcctg 30720ttttgaggtc aaacagaccc agctgggtaa ttggtgactc actcctggtt cctccaaaga 30780acaggggcaa gggaccaccc gggacctcag gtgccccagc acaggacctc tttctggggc 30840accctctggc actgggccat cttcaggccc tacagggctc tcggggcctg cgtaggcctc 30900tcatctccca gaaataagtt cttttcccca cccagactga caccagatgc tacagtgagg 30960tttacccctc cagcttgaca agcattccta gcccagaaca ctcagctgac gaggggtgct 31020gggggaggaa ggaggcagca aggctgaaag aagcttctct gggtattcca gtgcctggct 31080gctggtcgta gccatggtga gaccacagca gcctccctgg aagcaagcac ctgtgtaaaa 31140gcccctcctt ccccacaagg ggctggatcc aagtgaagaa ggtaccagaa ggactggaag 31200atgatgctgg tattattcga cagctccagg gtgctgtagc actgagcacc tactgtatgc 31260agcccatcac aaagccagcc acactgccca ggctgtgcca tggcaacttc cagcagtaca 31320ccctctcggt cacctgcagg ctccttgttc atcctgacac atgaggagaa aaagacagta 31380ctcacaaagg cctctagcct tgtggcccca ggaccctctc ctgagcatca tggcctccta 31440ggagacccag ggtagccagg acaaaggaga gaagcatttg ctaagctaag aaaggatgga 31500aggggattgc tgttctgcct ggatgcaccc ctagacctgg cagggcaggc aggagagacc 31560cccagtgtct cttgcttatc ctaggagttc ccccctgtgg ttgctccagt ttgctgcctt 31620catgcatgtt cacagggtga ttcccctgag acaacagagc tgggatgtca ccaagccctg 31680tgtcccatca gccaggctct ccccagcctc catcctctca ttctgctgga tcaggaggat 31740gtggggaggt ccctgactcc ccagctttcc ccatgacaag gtgtgtgtct acagctcaga 31800cccagccatg ctccacctga gaagtgggtc caggcagagc actgggtcct ggccacctag 31860ggctgcctcc tcaggggtgg gcaggaaggg gagccactgg aggagtgccc cgggcacagt 31920cccctgcacc aggtgaggtc acctcttagt ccatgaggcg atgctgcatg agcccctctg 31980gacactagaa ggcctgggct caatgctgag taaagtgcag gcatgactgt gccctcagca 32040tgcatagggc agcaggcata gattcataca cgtgagtttg cacttaagct gcagtaagag 32100ctacagagga aatgaacaga gacagcatca ctccagtgag ccctccagat acaccccatg 32160tgcgcagtaa gagaatggca tagtctattg ggacagccac atcaaaatac cacagaccag 32220atggcttaaa cggcagatat ttgtctctca caggtccgga ggctagaagt ccaagattag 32280ggtgccagcc aatccacttg ctggtgaggg ccctcttccc agcttgcaga tgtctctgtg 32340ttcccacagc atggtcacgt ggcagagaga gggggacagg gcatgaataa gccctcggtg 32400ccttcctctt cttacgagga tgctaatccc atcacgggta ccccaccctc gtgacctcgt 32460ctaaccctaa tcacctccca aaggccccat ctcctaatgc catcacactg ggggtcaggg 32520cttcaacaca tgaatttggg gagcacaatt tagtccacag cagaaaagaa aggggcacag 32580agcattccag cctgaaggaa acagatgtgc aaaggcccaa ggcaaaggcc tgagctctga 32640ggggcaacca gagtcaaggc tgaagagtgg gcagtgattg agcactgacg gtggagaggc 32700aacaggctat cttgttaatc tcagtctttt gtgtgccaga gagagaaact gaggcacagt 32760gtgtcatggt gaacactggg cagcactccc acgctcctgt cctgcactct gcagggttat 32820ggaggaagcc tgggaagtct ctcccctctt ttccaccttt cctggaccca tcccacaccc 32880tgcctcctcc aggaagccct ggggattgct catacactca catctcactc cttagtacct 32940gcagcctgca ttctgaattt agcaagctct ggccttgggt ttctattttc ttgtcctgtg 33000cagtgagcat ttggaaactt ctcctcagca ccaaacacgg agccatttag cagaaaatac 33060atgagttttc aagtcaattg aactgttcat cgccccaaaa gccaggcaac acggtagagc 33120agctggatct ctgttcgtag aagctttctc agggactggc ccaccgagtg ggggactgca 33180agtgcccgat gagtgaatga atgaacacat gtgtgaatgg aattcaaggc actgcaggct 33240attctggagg gtactggcct gtctcttcga caggtgaggt gcagtagaac agcatcccgt 33300atagatgcca gtttctgacc tggttctgaa aagccagtca cctacctctg cagagctgcc 33360tggagggggc gggggttgtg aggacccctg agagcttggt gtgaaggggc tctgcagagg 33420gaggcatggc tagtgtgcgc aaggtgacct ctctcctggt ctgcaggatg gagaacctgt 33480tcatcaaccg cttcatgcac atgttccagt cttcttggaa tgacttcgcc gactttgaga 33540aaatctttgt caagatcagc aacactattt ctggtgagtg tgcctctggg ggcccaagtg 33600gtgctgggga caggggatgc tgctctcctg tctgatactt gccgggaaat tgacaagggc 33660cttcctgcct gctgcagcat gggggcctcg ctgccaccat gccaccctgg acagcacccc 33720ctacactcca caccttttac catcactgtg gtcacccttg tgcccagagg tttctcctcc 33780tcagatattc aagctcgagc accctcaacc tcatttcact atcaaaagtc tcaaaagtat 33840cagaactttt tcttggcaca aatcaatcat ttgtgcttct gaacagagca aaggcagaac 33900ttggcttgtg ttttaggtat ttacacaaaa gcaactttgg gttcctttgg actaggatgt 33960gtttgatgag agcactagct tcatagtggc cagtcatggc atgggaagga cgttaggaag 34020gcaggaatgg ctggacaaga agaggtgctg aaatcaggac atggggaacc aaacttggca 34080aactgccccc agggactctc ctccacgccc tccttcttcc atcctggaca ctccatgctg 34140ggctgaagga cctgagtgct ccaggaggct tctgagccac gaagggccag ctcaggtatg 34200atgagttggt gccacagcct gacccagtga tgggcagggg gcagcagttg gagctgtgcc 34260catccaggga gcactcggga gaggagacca agcagggact ctgctcttag gtgaggtcag 34320gagggccatg gccctggctg ccctctactc agagctcagg gtgggcctcg cttttctcct 34380ggtagagcgg gtcatgaatc actggcagga agacctgatg tttggctacc agttcctgaa 34440tggctgcaac cctgtgttga tccggcgctg cacagagctg cccgagaagc tcccggtgac 34500cacggagatg gtagagtgca gcctggagcg gcagctcagc ttggagcagg aggtccaggt 34560aggggttgat gggctgggga agtggccaag gtcacagtct gtcaggtgga agccagttcc 34620tcctggccag tgctcatagg ccaccaagac gctaactgca ggcccatctg gcctacagca 34680gccgcttcct tttcctggca gcagtgtcag ccagggtcct gggcattatg cagactgtct 34740tgtgcaacat cagaggagga attgcgggga atgtttctcc atgatgctcg agtctgggaa 34800cataatgtca atattttcac tatcagtatc aataattaca ggagctaccc ttgattaggg 34860gcctgtggtg ggacaggcat tgtgacaggt gctttacaca caaggtcatc agttgtcacc 34920caccttgcaa aggggagaac actggagaaa gaagcagacc ttgtgtgaca atacacaagg 34980ggcacgagga gaacccaaca gaatggttga ttttctcgtt agaaatgctg ctgcccatgc 35040tcctgtcgca ccctcctccc caccctcacc tgcagactca gctgcctccg gctgcaaggc 35100tgacctagtc ttgagacaga agaagttcaa accaactcca cctggatctg gtggggctca 35160gcaacaggcg ctggcccatc agcctgcctc ttccccatgg atccagcgtg atggggcctc 35220ctgccaccca ggccctgctg cctatcccca ggtgtccagg tggcctctgc tgcttccagt 35280cttttgagct cagcttaccc tgaggcccta gttggagagg gatggttggt gccctttaga 35340gataaaccta cagccccagt acctggccca cctgaatcta gagggccacc cacccagctg 35400aagtccctgg gcaagtctcc tgccccagtg caggcagtgt ttcctaacct cctcctcctc 35460ctgcacccaa ccaggcccag aacacttcct ggtcatgtga acagctgaca gttacaatcc 35520cccacacact gagagagcat ctgtgttcct gagtggactg aattgctaga tgtagtaaca 35580ttgatttggt ggctataggt ttcaaacttc aagtcagaga tcatagaact ttaagccata 35640tagaatccta gaattggaag actctgagca gccatctctg cacccagcag ccaaggagtg 35700gccatcctca gcacctggca gggcatcagc ctcccactgg gagtcccggc cacctccagg 35760gcacgtgggt tatgagtcac ctttcctttg agctgaccca cccctcactg actagactct 35820taaacacaca ctgccccgtc taatacacac cagcatgatt cttaatcccc acagtcattc 35880acgctgccca tctggcagca ccttaatgat ctacagcaag cttgtggcct actaaaaacg 35940cctccattgt tttttaaaat gtgcagttat tttccggatc atgaatagag aaattgtatc 36000cctgttgttg gtattgattc agtgttccag ctcaagtttg agttcttaga ctttatattt 36060ggattctttg gtttcaaatc acaaaaatca actccatctg gtttaatcca agaactctat 36120tggctcaggc aacagtaaaa agcaatgcta actcatttgg gaccagggct cagatcatcc 36180cctgcactgt ctctctctcg ctccatgtct caatcctctg cctctggtga ttttttcctt 36240aggagggttt ccttatgtgg aggtaaaaaa tgatcaccgg cagcttcaga tttaccttcc 36300accagcttag caaacaccac ccactcccag caagaaagac cctgtttccc aatgctccca 36360gcagatgtct taggccatcc tcgggcctca ctgattctgt gagccagtgc ccacggccag 36420gagaatggaa tacactgacc tagaggggac agggactcta gacaggcaaa aactccactg 36480tcagaagcag aatcctaaaa tgtcagaact gaaaggtagg cccgcccctc gttttccaga 36540aatatgaaag ggaggcttgg gataacagag aaatgctgca ggcattggcc ccaaatctca 36600ggcctcctat tcccagtatc accttcacca gcctgggtgg tctcaagatt ggattagcag 36660ccattccttc tgggtagagt ctgatggtca cttcagctct caaagtccag ctatccagcc 36720atagtcctag aaggcaagac atcactgtgc ccgaccaacc caagggtctc cacctgcccc 36780cctcaacaca aggtgccctg agtgcctccg cggggatagt ccccgtcccc acccctgctt 36840cccccgccca tcgcgacaga tgctcagagc tcatccctgg tgtcacacca cataaaggag 36900gagggcggtg gccaggctcc gagctcctgc cgtcctgggt gggtggcttc tgtcctgcgt 36960gccgccgggc agcccggggg tcctgcgcgc gggcgcggtc ggtggagctg caggggggcg 37020ctctgggatg tctccaggca gagtagatac tgacccctcc gcagccggga ccctcaccct 37080ttccttcatc actcaggaaa cagaaaaggc ttcagaagga gcggccatgc ccccggccta 37140agcgctgcgt tcccggccca gcaggcctac cctgcacctg gcgttcctag gggcccctct 37200gcgggaccca ctgcgcggct cgcagcggcc gcccgcctcc cacttcaccg gcacgctcgt 37260tgcgtgtcga ggtgtgttga ctcctacagc aaagggcacg gatccgcggg tcagcaagcc 37320cgacgaattt acaccaatga cccgccgtgt cgccagtctc agaccagggc acaacacgcc 37380aaccctgcag accaccccca cctccccagc cactgcagac ccttccccct ctgcttctgc 37440agcacctgtc ccgaaacacg ctcattcccg cccccgcaga ccttccctac ctcctggaga 37500cccccctcaa cccctgtaga gtcccctgcc cgtgcagaat cccctccccc ttcccccgca 37560gcccccctac cccacccggc ggacagcccc gctcgcttcc tgttcttctc ccaagggaga 37620cccctgtcct ggctctgact ggggttgagt tcgccagttt tgacttatgt gtagagtctt 37680caccacgggc tggaagtacg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 37740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 37800nngagcgtac ttcagcccgg gtgaagattc cttacacctt atacaaaaat taattcaagt 37860tggattaaag acttacatgt cagacctaaa accataaaat ccttagaaga aaacctaggc 37920aataccattc aggacatagg catgggcaag gacttcatgt ctgaaacacc aaaagcaatg 37980ggaacaaaag ccaaaataga caaatgggat ctaattaaac taaagagctt ctgcacagca 38040aaagaaacta ccatcagagt gaacaggcaa cccacagaat gggagaaaat ttttgcacct 38100actcatctga caaagggcta atatccagaa tctacaatga actcaaacaa atttacaagc 38160aaaaaacaaa caaccccatc aaaaagtagg caaaggatat gaacagacac ttctcaaaag 38220aagacattta tgcagccaaa aaacacatga aaaaatgctc atcatcactg gccatcagag 38280aaatgcaaat caaaaccaca gtgagatgcc atctcacacc agttagaatg gcgatcatta 38340aaaagtcagg aaacaacagg tgctggagag gatgtggaga aataggaaca cttttacact 38400gttggtggga ctgtaaacta gttcaaccat tgtggaagtc agtgtggcaa ttcctcaagg 38460atctagaact agaaatacca tttgacccag caatcccatt actgggtata tacccaaagg 38520actataaatc atgctgctat aaagacacat gcacacgtag gtttattgtg gcactattcc 38580caagagcaaa gacttggaac caacccaaat ttccaacaat gatagactgg attaagaaaa 38640tgtggcacat atacaccatg gaatactatg cagccataaa aaatgatgag ttcatgtcct 38700ttgtagggac atggatgaag ctggaaacta tcattctcag caaactattg caaggacaaa 38760aaaccaaaca cctcatgttc tcactcatag gtgggaattg aacaatgaga acacatggac 38820acaggaaggg gaacatcaca cactgggcac tgttgtgggg tggggggctg ggggagggat 38880agcattagga gatataccta atgctaaatg acgagttaat gggtgcagca caccaacatg 38940gcacatgtat acatatgtaa ctgacctgca cgttgtgcac atgtacccta aaacttaaag 39000tataataata aaaaaaaata aataaaaata aaaaataaaa gaaaatgaag aaaatcatgt 39060catttgcagc aacatggatt tggctagagg actttatcct aggcaaacaa atacagaaac 39120agaaaatcaa ataccacata ttctcactca taagggggag gtaaacactg ggtactgatg 39180gacataaaga tagcaacaat agacactggg gactattggg gtggtgaagg agggaaggga 39240gaaagtgttg aaaaactgtt gagtactatt ctcagtatct gggtaatggg accattcata 39300ccccaaacct caacatcaca caatataccc aggttacaaa cttgcacaag tgccctctga 39360atctaaaata aaagttgaaa aagcaaaaat aaaacagtaa acaacaacaa caacaaaaca 39420gcaaaaccct caataaatac ataaataaaa atataaataa atgtctttgc cagagctttt 39480tgttttttgt gtggctttga gttagtatct ggggtcacgt tgcttttaag ctgtttgcta 39540tcaactgcat cctatctaat atttcttttt cttaatagta aaccactcaa tttttgtgag 39600gagatattac attgtggttt tgatttacat ttctctaatg atttcctttc cagtttggat 39660gctgagcatt ttttcttatg ttttttgccc attcatatat aattttaaaa gaaatgtcta 39720ttcaaatcct tttgccattt taaaatcata ttatttttaa atattgtgtt gtagaaatta 39780tttatatatt ctggatatta actctttatc agatatataa tttactaata ttttcttcca 39840tccacaagtt ttttttaaca ttgttatgtc ctttaatgca taaaagtttt aaaaagtttg 39900atgtagtccc aattgtctat tttttctttt gttgtctgta cttttggtgt catattaaaa 39960aataattgcc atatcccctt attctaagag ttttatagtt ttagatcttc catttaagtt 40020ttaaatccat tttgagttaa ttcttgtata tggcataaga caaagtttca acttcactct 40080ttcatgtgtg gatatccaag tttcccaaca ctgtttgttg aagagaatgt tcttccctca 40140ttgagcggat ctggaatgct gtaaatatta tttgaccata tatgtggagt tttcttttaa 40200tgggttcttt attctattcc attggtctat atgtctctct ttaagccagt gtcatgctgt 40260tttaattact gtagtcttgt aacatgtttt gagatcacgg agcatgagac cttcaactca 40320gttagttttc aagattgtct agtcagagta tatatgaata tttagataaa tttttctctt 40380ttttcaaaaa atgcagctta gattttgata gggattacat ataatttgta gactgctttg 40440ggttctattg acttttcaaa ttaatacata atgttgtaca gatttgggga atacatctga 40500tattttggag atcttaacat taaatcttcc aatccatgaa taccaatgtc tttacattta 40560tttgtgactt ctttcagtac tgttttgtag ttttcagtgt acaagccttt tatttccctg 40620gctaagttta ttcctaagta ttttactatt tttgatggta ttgtaattaa attattttct 40680taattacctt tggattgttt atagttaatt tatagaaagg caactgattt ttgtgtgtcg 40740attttggatc ctgcaacttt ccagaatttg tttaatagtt ccaacagttt tctggatttt 40800atgcatatat gcatctaaca gttttctaga ttttatgcag gagtctagga ttttatgcat 40860ataggatcaa gtcatctgtg agcagagata attttacttt tttcttttat tctttggatg 40920tcttttcttg cttaattttt ttttcctaat tggtctgctt ggaaattcaa atactatatt 40980gaatagaagc gaagagacag agtatacttg tcttgttcct aatccagaag aaaagtttga 41040atcttttacc attgagtgta agtttacctg tggatttttc atatatggcc tttattatgt 41100tattttcctt ctattcctag tttgctgagt gttttcgtga taaaaggtgt taaattttgt 41160taaacatttt ttctacatca atataaatga tcatgctgtt ttgtccttta ttctattact 41220atagtgtatt acattgattt ttgcatgttg tattattctt tttttttgag atgaaatctc 41280attctgtcat tctggctgga gtgcagtggc atgacctcag ctcactgcaa catttgcctc 41340ctgggttcaa gggattctcc tgtctcagcc tcccaaagtg ctgggattac aggcatgagc 41400cagcacaccc tgccacatgt tctatcattc ttgcattcca ggaataaagc caacctgatc 41460atcacaaata ttggtctgta gttttctttt catgtaatgt ctttgtttga ctttgaaatc 41520agggtaatac tggcctcata taatgagctt tgaagtgttt tttatattca tttttgtaaa 41580tgactttgag aaacattggt tctaattctt ccttaaatgt ttggtagtca tccggttctg 41640ggctgttgtt tgtttggagg tttttgaacc agtttaatct ccttcgtagt tataagtctg 41700ttcgtttttt ctacttcttc atgattcact cttattaggt tgtatatttc tagaaattta 41760tgcacttcta aattatccaa tttgttgaca tataattaac tgttgatggt attttcttat 41820aatccttttg atttctatgg catgggttct aatgttctct tttttatttc tgatttatta 41880gttgaatctt ttttccttag ttaatctagc taagcatttg ttaattttgt tggtgttttc 41940aaaaaccacc tcttagtttt gttgattttt ttctattgtt tttatattct atatttcatt 42000tatctctgat ttaatctaat ctttaggatt ttcctccttc tgctagcttt gggtgtagtt 42060ttttttctct cctagtttcc tgaggtataa agttaggttg ttgatttgca aactttcttc 42120tacttctttt tttttttttc ttaagttgtg gagatggggt cttgctttgt tgcccaggct 42180ggtctcaaat tcctggcctc cagaagttct cctccctcag cctgtcaaag tgctgggatt 42240gcaggcatga cccaccatgc ctagccactt tcttcctttt ataatgcaca tatttatagc 42300tataaaattc tctgttagca ctgcttctgc tgcatcttac acattttggt acattgtatt 42360tttattttcc tttatctcaa gatattttct aacttccctt gtgatttctt ttttgaccca 42420ttgcttgttg aaaactgtgt tgtttaattt tcacatattt gtgaattttt tgttttcctt 42480ctgctactga tgtctagttt tatgtcattt tcatcaaaat agatattttg tataatttca 42540atcttttaaa atttgttaca agtttttttg tggtataaca tgtgatttat ctttgggaat 42600attccatgta aacttgagaa aaatactatt ctgattttat tgggtggaat gttctgcaca 42660tgtttgttag cactatttat agtgttgttc aagtactgta tttccttatc gattttctgt 42720cttgttgttc tagccattat tgtaagtgag gaattgaagt cgcctaccat tattatgctg 42780tttatgtttc ctctctgatc tgtcaatgtt tgcttcagat atttaggagc tctggtatta 42840gaggtatata tatttgtaat tgttatatct ttctgctgaa ttgacttttt gttatataat 42900atccattttt ttgtcttgtg actatttttg actttaagtc tattttgttg gatagtagta 42960tagtcacttc tgcttgcttt tatttaccat ttgcatggca tttacttttc ctttttttca 43020ttttaagcat atttgtttct tagacctaaa tgacaggata aagttggatc ctttttacaa 43080atccattttc cagtctatat cttttgattc aggagtttaa tccatttaca tttaaagtaa 43140ttactgatag ggaaggacct acacttgcca ttttgttgtt tcctgtatgt tttgtaaatt 43200ttgtttttta ttccttctct tactgacctc ttttgggttt tgtcgatttt tttttgtggt 43260gacattttga ttcccttcct gtttcctttt gtgtgtattc tatagatttt tttatggtta 43320ccataagaat tataaaaaac atcttaaagt gttaacaagc tattttaaac tgttaagaga 43380ataacttcga ctgtgtacaa aaactatttc ttcaaagctc ctaccctcac cttaggttac 43440ttatgtcaca agttacatct tcatatgtaa tgtattcatt aacacagttt tatagttatt 43500tttaagcttt tgtcttgtaa attctataca agaattaaaa gtgcatttat gtaccaaaac 43560tgcatagtac agtctctata tatgtccata tatttatctt tactggagag ctttatattt 43620tcataagact tcatgttact atttagcatc cttttgtttc aacttgcaag atactacagc 43680aggtccagtg gtaatgaact ccccagtttt tgttcatcct ggaaaggctg atttttcctt 43740tatgtttgaa ggatggtttt gctggatata atatttttgg ttgaaatttt cttttctttc 43800agctctttga atatattatc cccctgcctt ctggcctgca aggtttctgt tgataaatcc 43860actgataatt ttataaaggc ttccttttac atgacaagtt actttctttt gctgctttca 43920agattgtctc tttgtctttg acttttgaga gtttgattat agtgtgtctc actgtgagtc 43980tctgggtttt ctttaacatt ttttttttgt tcatatcttc tgatgatgaa ttcctttaac 44040ttttatattt gtgaaatgtt ttttcttcat tttcagaagg tatttttagt gagtgtaaaa 44100ttctacattg acagtttttt tctttcagta ctgtttagtg tttctctact gtcttcttgc 44160ttgtactgat tgtatagaaa ctctgctgta attcttatct ttgtgtcttt gtacttaatg 44220tgccttttta ctctgcctgt ttttaagatt ttatttttat tattgatttt gatcatgtgg 44280cttggtgtga gtttcttcat atttattgtg cttggggttc attgagctta ttaagtctgt 44340aggtttatat ttttcattac atttagaaaa attgtagcca ttattcattc aaattttttt 44400ctgttcttct cccatttctc atgcccttat ggggtctaat tatatgtata ttaggctatt 44460tgaggttgtc ttacagctca cttacagttc ttatgtttat ttttccactt ctatttcatt 44520tgggcaggtt tattatactt ttccctaatt tgctaatttt tttgcaatgt ctaatccact 44580gttaatctca tccaatgtat tattaatttc agacattgta gttttcatct ctagaaattg 44640ttcatctttt tatatcttct ttgtctctat ttgatatgct aagtcttttc tctagcttct 44700tgagcatatg aaatatagtt atgacaacta ttttatttta ttacaacttt taccttaggt 44760tcagaggtac atgtgcaggt ttgttatgta ggtaaactcg tgtcacagga gtttgtttat 44820tatttcatca ctcaggtact aagcctagta cccaatagtt gtttttctgt tcctctccct 44880tctcctaccc tccaacttca agtaggcccc agtgtttatt gttcctctct ttgtatcact 44940actattttaa tgtctctact agttctctta tttttgtcat ttctgagttg attttatttt 45000tctcactatg gccatattct cctacttctt tgaatatctg gtatgcaaat tatacctagt 45060atggatatta tatcaagacc ctgtcgcaat atggatacct aaaagaaaat aacaatgttt 45120accagtttgc tctaagcctt tcttagctct tttgcttgtt tctcaagaac aagcacagag 45180ggtggagctc aaccaagact gaagttaaag atgtagaaag attgagatgg gaagtaatta 45240agttgaggag ctctgataca atggtatttc tctttttaat catgtaagat gcaagcccaa 45300gataatatgt tgggaaagtt gaaggcgaat gtcttgagaa atatgaaatg cacccaaggc 45360tatggctggg tgatagaaat aactaaaaag ttttaaaagg attgttttat agctggtcct 45420tgctgaggtt aggggatagg cctggaaaaa ttgagcagtg gtattgccca ggcaggactc 45480ctcatgacat tctccagatc cctaagtttt tccaagtttc caagcatttc cttggccagc 45540aagtcatctc gcagcacaga tcctactctc tttcagtcag tcattccaca actctacaaa 45600gcatttttct ccatcaaatg ggaacttttg gggttctgca taatttttca gggttccacc 45660acttttgact ctttctggat accttgtaag gatattataa ctctttaatt catgtccttg 45720attttctctt ttaactttta atactgttca ttgcctgttt acctctttca ccctcttctt 45780ttcacttccg ccctctttct tgtgtaagta tataatcatc ctttttttct tcctcgaatc 45840tcagttctcg ggagtaacat atacaattca ttcttacttc cagtctttta ttcctaagtt 45900ccttatctct agttctcttg catatttgtg gcaattatta gtttttttcc aaaaatccat 45960tctccctttt tcctgtacat gtatctttcc agcttgacca catttcccag cttccctggc 46020agctaggtga agcaatgtaa ctaaactttt gcctgtaaaa tatgagcaga agttatgtgc 46080acaatatctg cctcacttgc ttaaaataaa ttgtattatt tgagctttct atattgtccc 46140tttttacaac taaaatgtag aggttatggc aacttttatg gtaaagagaa tcatcacgta 46200ggagatcaag aatcattgat ttgagaggaa cctaaatcct ttaatgactt agtggagcaa 46260gactgcccca tcagtctgga agctacccca caacttatgt atatcttcag attgttgagc 46320aaaagagaaa aaatgaagcc actatatatt taggtctctt catgttaaca gtttagcttt 46380taacctaaca cacatttatt cctgaatggt agcatattat taacatcaca ggaagggaat 46440tccttgttca tggatctttt attttgttga aataaaggta atctgcttgg gcttaatact 46500gccaaggcag aattttttgg aataattatt tctatagatg acccattcat aatgacaact 46560aacatggttt ctaaaatgga ttcatgtacg tcatcttatt tagtccccgc aaaaattctg 46620aaggtaggta taattattcc cattctatat attgaaaaat tgggattcag agagactacg 46680gacttataca agtttctgca gctatttaag aaacagagta aagtctcaaa gccattaatg 46740aactgtttag tttagtgctg cttagtaaaa ttagaactgt ttgaagtgcg ttttaatagt 46800aaacccatcg atatttagtc agctctctgc ttgctaacta atatggaaga aaaaacatac 46860atctacgtac atcctgaatt gggagaaggg aaataaaata ttgccaatcc cctttcctgt 46920attaggtact gtgataaggc gataaggctt ttcattatta taaatgagtc ctggagaggt 46980taagcgcagt cacccaaagt cacatgacca gtaactttca gagattttac cccaggtctg 47040gaaagactct gaagcccatg ttattgcctc cagagcctgg tggagaaccc ctgaatggga 47100cagagactca gcagacagag tggtcctttc cttccaggga gtcagggaag gtttggtagg 47160ggagatagag ttagatctgg gtgggatttg gatgaaatga gatcaagcag ggataaataa 47220gatccaagct gtttatgagg gacagggaga ggccagctct gttagaaaga gtggtttata 47280cagagaagca tggaaacatt tgctccatgc ctcgccttcc atggatagca gtccagaaat 47340gctacttgcc tgcctgacag tctttctcca gtccccaggt gtggacacgt tctgagcacc 47400tgagcctggg tctgccctga cccagattct gtgagctggg acagagaatg tgtcctgctg 47460tccctcctcc cccacttggg cccacaccct tttctacaca tttggattag aaatctgaat 47520tggcctttct gtgaacccca atgacaggca agtccccagt aaagaagcac ttaatttaat 47580tgctccttca gggtccactt agagtaatag ttgtgaacag ctctccctag tgtctcccac 47640aggcagtgac aaatgttgct aaagataagt cacaaattct tggcaaggcc atcttaaata 47700tgggaaggaa accttgttca gtcctgaaca gtcctgttca gactgatctt atcacaggtt 47760gcacactgcc tcaatgtcac catgtttcta ccctcagttg tgcttggcag agagaggaca 47820gctatacata aagtctgttc actctacaaa ggtgaaatca ttttttttaa ggtaattatg 47880attgttctga ggatggtgat tgagtgacat ttaaaaaatg ggaaaagttc tactcataac 47940tctgtctgct taatttgctt atataaaaga cttaatggtt tttgttgagt tgatccagag 48000tcagtaacat ttgaatatgt gttattacca tttgatcagt aacaaaacaa cagggtaagt 48060gggatgccaa atgtggctga gagtttaggg tttacattcc acaccccagc cttaaatacg 48120tttgtataaa ttattggttg atttttcttt ttcatggctt ttctttgaca tttgatgggg 48180ctggatttat ggaggcaacc aacaaattac acagcaatga attctggact gtagtcccac 48240agccttgagc ctagatacta gctgtgtcac taatactctg tgtgaccagg gtaagttatt 48300tagatacatt tatgactttc caaatcaata ggttggacaa agttatcttc aaaatgtctt 48360tcagatttga ccctgaacat atgcgattct ggcatgttct gatttttagt tttctgttct 48420tgaagcatgt ctacaataga aatgcctacc ttcatataca ataaaaggac attagattaa 48480tatggtgtct gtgaaaatca ttgaacagct ttagagataa aaataatgaa atgagtcaag 48540ttctccatta ttaattaaat cttttaaatt ttctgtttat gaaactatga acaataaatt 48600ggaacaaaga tataatctca ctacaagtaa attgtatttt atgtaaaata taatttgtct 48660ttagaaaata ctcaatgaaa ttaccttttt aatttcagag attttgagga ctattattgt 48720tatttcttta aagtgataaa tgtaatccaa gatgcctttt ttcccccttc agaagcttga 48780gtgcccaagg gtttgataaa tgtggaaaat aaccgctgtt cgaatatcct gttggcctgc 48840tggtgagatg tcatcgtgtt gtctgaatgc agtatgtgtt taaacttgcc atttattacc 48900tgaaattgtg ttattttgcc tcataccctt agtaatactc ttgtaatgct ggctgagaat 48960tgaagcaaat gccctatgaa actcaaggga gcaaggtctt tgttattttt cagaagaaat 49020gactggctga ggctgtaaag aaatcttgtg gtcattctca tggaaagagg attggagccc 49080cgattctttg atctcattat tgaccagttg attgagaaga ctgtcctcct gtccccaccc 49140aatcacaata agtgggtaat tacctgaggg ctaatttgct ccttgaatgt gaatacattt 49200gtggaaattt ccttgtgctc agcacctgtt caaaacatcc atttgtattg cagagctatt 49260gtttttctaa tctcattgtg gagctaagag agagattgtg tttaaggttt ccttcgtaat 49320aggttttctt gctccaggaa ggatgcgtga cagactctgc acactgctgc ttggtaggtg 49380ttcagtgatg gatgcgtttt ccatctccca ggtacagctg cagctgtgct gactctggtg 49440cgtttgtaat atgcactgtg gagtgcacca tgatttcatg atggctggag aaatctggtg 49500gctagaattc tagggaggag gacatgagtc ggaagtgcag aaacctcaca ctgggacatt 49560gatggagaaa atccaattct gtccctggag ttaaagagga gtcactcact gggccatcat 49620aagagcaaat aaagttacag ttatttcttc agatccacaa ctcaaactca gcgaaagtaa 49680ttattcactc tttttgtccc aaacaataat gaaatcagct aaagcatatt gtatttatta 49740cttccactca agttattgag ctgttctgac accttgttgc tgggaagata cacagatgga 49800acaccatcat ttccattgat tctgatcata ccccacgcta atatagcctt attgctctga 49860gtcataggga ttttatgctt gcctattcaa aaaacagttt ttaccttatt aaaaggtgat 49920atgacctgag ctggagatga tgtttggtca ttttgtgtaa accttttaat attttagtgg 49980taaatacatt tcttgaatct tgacaaaaac ataattaaaa tgaacatatt taaaatggac 50040atcttccaaa tgttcatttt taaaacaaac atcttccaaa tgttcatttt taaaatgaat 50100atcttcgtta actttttttt ttttttttga gacggagtct cgctctgtca cccaggctgg 50160agtgcagtgg cgcgatctcg gctcactgca agctccgcct cccgggttca cgccattctc 50220ctgcctcagc ctccagagta gctaggacta caggcgcccg ccaccacgcc cggctaattt 50280ttgtattttt agtagagacg gggtttcacc gtgttagcca ggaaggtctc gatctcctga 50340ccttctgatc caccctccac ggcctcccaa agtgctggga ttacaggcgt gagtcaccac 50400gcccagcctc ttccttaact tttaatgaat aaagagttga aagggaaaaa ccaaatgtgc 50460actatgctga ttttatcttg agctgtacag aatgtgcagt aacttaagaa tataagtcat 50520tctgctgtta ttcatactag cctattcttt agcattattt aaacacacat atttgagtgt 50580ctacacactg cctgctaggc agggggctgg gtgccaagaa gggatgtcca gagataaacg 50640ggcatatgtg ttaatctttt agggcacagt ccagtaacct gatcaaggag tgacgtgtat 50700tgtgtactag gatagaagtc agtatcagag gaaacacaaa ggaaagagtg gtactttctc 50760cccagggaag ggatgctcag aaagaggggg ctgattatgt ccctggttca tgagaaaaag 50820ggcattcctg gctcagggag tgggaagaac aaaaccaaga aatcctggaa tggcttggtg 50880catcagccag tctgtgagtg cagggcatgg ctggagttct ggctttaaga tgagtgggaa 50940gaaaggctgg agaggaggtg gatccaggaa aagttgctgg gagcatttat tcttttggca 51000atgggaagcc atggaagaac tgttgtaagg aggattctgt gtgatacatc tgccttttag 51060agaggtcatt ttatagaaaa agtagaaaat acagaccagc aaaaatttaa aaagtgaaaa 51120caatcactgt tcaagctctg gtctagcact caccagtgtt tttcctacag gtgcatatac 51180aaacatgctt ttacatttta tttttatttt aaatttttca ctatcctcaa tatcagtgga 51240aacaaatctg cttttttaat ttttttttta aaaatgagat cctattgtac acattatttt 51300aaattttttt tcattctttc ctttttttaa aattaaaaca attttttttg agacagtctc 51360actctgtcgc ccaggctaga gtgcagcatc gtggtctcag ctcaccgaaa ccttgcctcc 51420caggttcaag cgattctcct gcctcagcct tccaagtagc tgggattaca gacatccacc 51480accaccgtgt caggttaatt tttgtatttt tagtagagaa tggggtttca ccattttgga 51540caggctggtc ttgaattccg cggatccttc tatagtgtca cctaaatgtc gacggccagg 51600cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcgtattc gcccgtggtg 51720aagatcgtag tagagtctac cacctcggcc gtgaacctgg ctcagggggc gcagccgggg 51780tgggcgaccc agccaggtct gcggagcggt gcccggcggc aaccccctca cggacgctgg 51840gactccccca ccccgtcccc attcagcgct agcggtggag gggccaggcc cgccccaggt 51900agtcaggcag gggtcccggc tcctccccca ggaccaccgc cccgcgggcg ctcacgtcct 51960gatggcttcc cacaccagga gcccgtcgtc ccggtagaag tagtagggga tgtcttcttt 52020gctctccatg ccccgggcct tgatggcctc gggaaagcac agggaggcat aggtcaggtc 52080cttcatggcc ctctgcacca tctgcacgtg cccaccgccc cctgtggcgt tggcctggag 52140tgggaggtag agtgccctga gcgggtggct cctcccacag ctgtccagac ccatccctga 52200ggaaatgtgc caggggaggc gaggaggcca ggcaggcaga ggtgcaagcc cttgtcctgt 52260gagatggggg agggatgttt aacgcagaga atgacaaggg cagccgggtg gagaggtgct 52320agggaggagc ctcccgccca atccaatgca agggactgaa ccgtggggct gcagagtaca 52380agagggaagg gtgcgctccc agccacagga gggcagcagg cgtgggcagg tgtgtggggg 52440accccagcac cagcgggcct gggctgtgaa gtctgctcta gtgctgagca cacgaccctc 52500agtcattgct ctcagtgacc ctcagtttcc aggacccatg caactctgtt gagggcctgt 52560tgtgtgctca ggtgagggct ggagagaccc acaaggaggg atcctggtcc tgatcccgat 52620cccggcttct gagtgatgca gacacactca caagctcact ggcacatatg tgtgtccacg 52680tgcacacaca cacatgcaaa tacacccagg gggctgggct gtcggtgcca cagaggtttt 52740gaagaaggac ttgggtgctg gggtgccaga gattggtcca cggtgggcat tcagcccaag 52800caggaccaac cagagctgcc ccccagcact ttctgtttag atgcggctgg gagataatgg 52860ccacatgaga acctgaggtg gagctactat gatgagctcc tgtgccctct cccatggggg 52920cctgtctgca gcaggagagt gtgatgccaa cacaggtgaa agagacagaa agagggagcc 52980agtgtgcatg caccctgggg cattgcttga gtccctggat ccatttctgg ctgaggcccc 53040cgaccctcta ttgtgtccag gtatttgaga taaggcatgt cttgtccagc tgcagctggt 53100ttgagttggg ttttgtcatt tgcagcccaa acagtgtctc aacttcagtg gtcatctggc 53160ctggtcacta atgggctggg gaaaggagga aggttggggc aagggcccat gtgagagtcc 53220aagcttggta aggctacaga gggctcaaaa cgaggtgggg tgctacctga gggatgcgag 53280ggctgggcag ggcctggagt ccccgatgca ggagacagtg ggttgggagg gttgggaccc 53340gggaagcaga gtgctgggga ggtccaggtc agggtggcag gggtgtggca gattaagagg 53400cagacacagg ttcctggtgc gtaggaggtc agggggccat aaggagtgtg ttccagaggg 53460gggtctacac ccaggtccca gaggctggga gaggggaggg gtgggaggag gggaccagga 53520gccatcagtg agaaggaaga cctggtggtg cgcctggagg agtgctcctg gctgaatggc 53580acgggctgga gacatcagga ggatgctgga gccagtgctc tgagcagact ccagccttca 53640cccttccagg cctcctgaaa gggtgtctag atctcagata gggagtgaag gcggccaggc 53700tgggctcttc caggcaacaa gtagggtagg agggcaccca ccttgtcaaa gaggccacac 53760tcgcagatga gctgctcacg ggccttggtg ttgattgcaa tggtgaatct cacgtgtgcc 53820accagcagct ggggagggga gaggaggagg cctcagaggg ggcccagtca ggtgacctgg 53880tgcctcaggg acccgcagct cccccaccca ggcctgccct ataggatgat gctgggcgca 53940gggaggaagt gaaggctggg tgctgatctt tctgctgaat aagtgctgga ggcctgcacc 54000cctctgatgc ttactaagca tgataatgat tcagagggtc ctggccagca cttgtacaat 54060gcttacctct ttgctccctg agttcactgt cttaatctga ccatcattct gtaaggcagc 54120tgtactattt gacctgtttt actggtgagg aaactgaggc acatgggtac ttttagcatg 54180cagtcacccc agcagccaat gcttgtagga ttcgagaaca gggacggaga gtgatggcag 54240gggcggccat cagcatcagc cgaggatcac catgaactca tttgcctttt ccaactctac 54300tggctggcca gcgaggcccg tgtgtcaggc ccatgcccca gccgcgtgtc ctggggaaca 54360gcaggctcct agctgttttc ttcctttagg ggcattactg actctgagaa catctctcca 54420gggctccctt tctgccaccc actcccagcc acaggtcccc ctgtgggatg tcagtgatcc 54480ctcccactcc acatctcaga tgccctccca tagcaggtgg ggcggtagct ggctgtacct 54540tgaaaatggg gtgcacagca ggcagctggc ggtacattgc aatgccaaaa acctcagaca 54600ccagatgtgt tcgcagaagg tgggtgatgg tctggtggac gtggaagtca ctggaacgca 54660cccagatttt ggccaaaagc cagtcgtatt ttgcatccga agggaggaaa atagggttct 54720catctcccgg gatttggttg agctgataca ttgggggaaa ggaaacacac tgctatattt 54780cacttcagag tatagttgtt ggagaacttc gcctcttggg aagttccaga agtatctgaa 54840tttctttaac agtgactttg gaatcagaag agcctggagg gggtaaaggg ccctgcggtg 54900gtatttcctg tcccagccat attacttaca acctgcatga ctctggatga cacactgggc 54960tttctaagct ccggcttcag catctgtcat gtggggacag tgatcgctcg gctcacagtt 55020actcaggagc tggcagcatg gaaggagcgg gcaggccgaa ggcccagcag cggaggggga 55080ggatcgctgt ccccctcttc cctccttgcc ctgctccctc ttctactgcc tctccttggt 55140ctctcatact ttcctccctc tcctcttccc tgaaatgctg gagctcccca gggtctgcca 55200tggtgctcct gtcttctttt cttgccctaa gtcagctcct cctgtttaaa ggcttggggc 55260cctcctggta ctgagcctcc cagatcaggg tctgcagctt gagctctgcc tggagcccac 55320acctgtggcc aggcacctcc tgtagcagag gctggacctg tcttcccttt ccagtttcca 55380ctccacactg ggacaaggtg agtttttttc ttttccaaaa tgcaaatcat gtcatgttaa 55440ctgcctcctc aatcacctct taatgccaga aaataaaatc cccaagctct gtggtgacct 55500ggttgctgat gcctctgtac acacagcctc actttcgtgc ccccacacca gcacccagcc 55560ccaggtccct ccatgccagc agccctgcac gttgccccct tattgagagc ccagcacagc 55620ctcagcaccc gacagtgagt tctcacggga gccggcagca ggtgctggca ttgcccattt 55680ttacatgaag acatggggta ttggaggggt tgacagaaag aatgaggtgg gtggcagggc 55740tgcagccaag agcggccagg aaggggcatg gccagacttt aggtgctggg aaagaccagt 55800gtcactgcag cacagtggtc atggctgggg agggagaact gtcccagagc acacagctgt 55860gtcacttgct ataagtattg tggaaggtgg ggtccttggg cgtctctccc tcgaaaacag 55920tgctgtccaa tagacatata atgctacccc acacatggaa ttgtatatat tcaggaagcc 55980atattaataa aaataaaagg aaacaggtga aatgccggtg aataatataa ttttatttta 56040cccaatatat ccagaatatt atcacttcaa catgaaacca atctaaaact attaaatgag 56100atatttcaca ttccccacct ccttattcta agtcttaaaa atcaggtgtg aactttgcac 56160ttgcagcagg ttttggtttg gacgagccaa atcccaagtg ctccagagcc ctggtggcca 56220gtggctccac cctggtcaga gagaccccag aagcctcagc actccctgag gaccaaggag 56280gggggaggga ggagggagag cagagtgtga cttgcacatt aggcgtgcct gtctgtttat 56340acttgagatt agttggctgc ttttaaatac cagacagttt cacataaaaa tttgaatgac 56400ctggttttct tgaaaaatta gaaggtctca gaactctgca cccaggttct cacagaacta 56460cgctcttgtc ccttttacag agtcccagct ctcagggtcc ccactgccac acccgtttgc 56520atgagatgcc tgggccactg atacctgatc tgtgccctag aatgctgggc tgcctggcat 56580gctctgagac actttctgac atgtccccac atgctctgac atgttcctga tggctctggg 56640acactgacat gttcctgatg gctctgggac actgacatgt tcctgacatc ctctggggcc 56700gaggagcccc cctgttgtgt gctaggggct ttacccatgc tatctcactt aatcctcact 56760ttgaccctgc aaggcagata ttccttgctg tacccatttt acatgggggg aaactgaggc 56820tccagctggt ttagcagctc tcccaagtca cacagtaggt aaagagctca gccggcaaca 56880tgatattgaa caggcaaaaa ttggaagcaa tccccctaaa aactggaaca agacaaggat 56940gtccactctc acccctccta ttcaatagag tactggaagt cctagtcaga gcaattaggc 57000aagagaaaga aataaaaggg atccaaatga aaaaaaaaaa aagaggaagt caaattatct 57060ttgttcatcc atgatatgat tctataccta gaaaaccctg aagattcctc caaaagactc 57120ctagacttga taaacaactt cagtaaagtc tcaatgttat aagtaaaatg cttgtttaga 57180aacagaatgc ttgttcctcg gtactgcaag gaaaaatcag catttagaca aaaagttttt 57240ttagcaagcc aattttactt tctgcagaaa gggtgctcct cgcagatgga acaatggcga 57300gagcacacct gaataatgga gagaagcaat ttttattcct tacgcagctt gtccctgcta 57360ccgtgtcctg tctccattgg ctggagccaa acctcacagt ctaaactaaa acccgactgg 57420ctaataactt aaaacttttc taaataggta aaagcagtgg aaagacaaag gaaaagagga 57480agttgcttat gaaaggactt agaaaagtaa taacatttcc aaataaggaa ggggcatggg 57540ctgcaagctg ggacatgcct gtgagcacgt ccagcacaaa tatcttggtt aaagtacaag 57600gacatagaat gtactcattc ccttatatct aacagctaca tatgataggg cttaacaaaa 57660agttattagc acaaagcgaa aaggcttaaa aaaagttagt ctttagaaaa aactattatt 57720tctaacacat aatttactcc ttaacaaaaa aaaaactttt gactttctac actcaagata 57780caaaatcagt gtacaaaaat cagtagcatt tctttttatt tgagacggag tctcactatc 57840acccaggctg gagtgcagtg gtgtgatcgc agctcactgc aacctccgcc tcccaggttc 57900aagcaattct cctgcctcag cctcccaagt agctgggatt acaggcaggt gttaccacac 57960ctggctaatt tttgtatttt taattgagac ggggtttcac catgttggtc aggctggtct 58020caaactcctg acctcaggtg atctgcccac ctcggcctcc caaagtgctg ggattacagg 58080cgtgagccac tgtgcctggc ctaaaaatca gtagcatttc tatgcaccaa caatgttcag 58140gttcaggctg agaaccatat caggaacgtg gtgatctcag ttacaatagt cccccataca 58200caaaataaaa taccaaagaa tgtatccagc caaggaggtg aaagagctct acagagagaa 58260ctataaaaca ctgatgaaag aaatcataga tgacacaaaa aaatggaaaa acatcccagc 58320taatgggttg gaagaaagca atctacagat acaacacaat tcctatcaaa ttatcaacat 58380catttttcac agaattagaa aaaaaaatcc taaaattcat atggaaccaa aaaatagccg 58440aatagccaaa gcaatcctag gcaaaaatac aaagctggag gcatcacatt acctgacttc 58500aaactatact acaagactac agtaacaaaa acagcatggt atactggtac aaaaataggc 58560acagagatca atggaacaga acagagaacc cagaaataaa gccatatacc tgcaaccaac 58620tgatttttga caaaatcaac aaaaataaac aataaggaaa ggacatccta ttcaataaat 58680agtgttggaa aaactggcta cctatatgca gaagaatgac gctgggcccc tacctctcac 58740catatacaaa aattacctct agatggatta cagacttaaa tgtaagactt caaactttaa 58800acattctaga agaaaaccta ggaaaaaatc ttatggacat ttcctaggca aagaatttat 58860gactagacct caaaggcata tgcaacaaaa acaaaaattg acaaatgaga cttaatcaaa 58920ttaaagagct tctgcacagc aaaacaaact atcaacaaag taaacagaca acctacagaa 58980tgagagaaaa tatttgcaac tatgcatcca caaacgacta atacccagaa tctatgagga 59040acttaagaaa aaacaccacc attaaaaagt gggcaaagga caggaacaga cacttctcaa 59100aagaagacat acaagtggcc aacaaacata tgaaaaaatg cccaacatca ctaatcatca 59160gagaaatgca aattaaaacc acaaagggat accagtcaga atggctatta ttaaaaagta 59220aaaaaatgac agatgttggc aggatgcaga caaaagggaa tgcttacaca ctgttggtgg 59280cagtgtaaat tacttccatc cctgtggaaa gcagtttgga gattttgcaa agaactgcaa 59340atagaattac cagttgaccc aacaacccca ttactgtgta cctacccaaa ggaaaagaaa 59400ttgttttacc aaaaggacat aggcacacat atgtttattg cagcactgtt cacaatatca 59460aaatcatgta atcaacctag gtgcccatca atagtggcac cagataaaga aaatgtgata 59520catgtactcc aaggaatact acatagccat aaaaaagaat gaaatcgtgt tctttgcagc 59580aacatggatg cagcttagga ttggcactta agccataatc ttaagtgaat taacccagaa 59640acagaaaatc gaatactgca tgttcttatt tataagtggg agctaagcat tgtgtacaca 59700tgggcataaa agtgggaaca aaagacactg gggactccag gaaggggagg gagggaggaa 59760agggttgaaa aactgggtat tgggtactat gctcagtatt tgagtgatgg gttcaataga 59820agcctaaccc cagcattatg caatataacc atgtaacaaa cctgcacatg tacctgaatc 59880taaaatttta tatattaaaa aaaaaaaaac tgttctgacc agtaaaaaaa gagctgaatg 59940aggatgggcc cagtgctctg cctatttcag cccatcactc tcccttctgc ttctcttcac 60000ctcccggcct gaccgggcct cacctgccca tctccttgag ctcttgtacc actgagctgg 60060gccttcccca acccatcctt cacatcctcc cactcctgtc caggattttg gcctgccatg 60120ggatgccaga acttgccctg agcaatttgt gtttcttaaa tttgtttttt gggtaaggaa 60180gtagctttgc gtttctgatt agaaaagaat atgtgcttat cgtaaaaaga aatctgagtc 60240atgacaggag agcctgagaa agaaaacatc atctcatctt ccagcgccca gggataacca 60300ccaaattctg gggagcctcc tttcataggt ctttgcatgt gtgcacattt atctgtccaa 60360ttttccatca actgaatatg attataatca acatctgtct ctttgttttg ctcacctgtc 60420ccccactggg ccatcactgt gttcctacct ggtgacactg cgtccttgtg tccccatgct 60480ggcacagtag gcctgctgca tccttgcatt cattttacat aagtgagacc atgcccattg 60540ccttagcagt ttgaaggagc tatttgtata cgacagatat gtatccttta tcggtccctt 60600gtgtcttgtt tttccactgt taactcattg tggagacccc tccaggtcag ctggaaggga 60660accaaagcat tcctttcaaa gacgcctcaa cattttatgg tgaggacatg gaagggctca 60720ctcactcaat cctcttctgc cttcagactg ttttcaattt ttttccccat cacaaactat 60780gctcctgttg ctacccacac acatttgtgc tttcatttct gctgagtggc tgctagcgaa 60840ggctgagcct tgggggtttg ggtatgtttt cgagtagaca gtgcctgccc tactgttcct 60900cagacagttt cactctcctg gtgcacacct gttcagtgct actggctgtc agaacactta 60960aacaataaag tcccctccag tgctcctcca attgagacgg ggcaggggcc tcctcttagg 61020gcactgtagc cccccagccc cgcaacaggg catggaaatg aaggaaaatc ccgagttcct 61080tgaagggaag ttccaggcac ctagctagcc ctgagaaata aatgagcaac ttgacaaaca 61140agagggtaac agcagcctaa aacaacagcc aaggaagcca gagtcctggg ctgtttggtt 61200ccctctggaa actaaagaga acccctgaac atgtgtctct gagttgtttt tcagaaaccc 61260agacccccac caaaccgatc cactggcaca gagacctcag ataagacgga actgaggact 61320gaactctgag caggctcttt gttctaaatt tcttcctggg gggcctgggg gaagtcccgc 61380ccacaagcca cagctaacat tcctttctgg taacccaaat ttttaaatga aacttctctt 61440ccttaaccaa tttcaaaaca acaaattttt gaatctacct atgacctgga agcccacccc 61500cacttcaaga tatcccgccc ttttaggcca aaaccaatgt gtaccctcca tgtattgatt 61560aatgattttg cctgttaact tctgcaattc ctgaaattca ctctgccttt aaaaaccctt 61620acctgcaagc catcagggag ttccggtctt aagagtgagc tgcccaattc tccttttttg 61680gtccccgcaa taaatgcctt gtgttctcgt agattatatc ccaatctcag tgtttggttt 61740tgctgcactg ggcaagcaga tcccagtcca gctcggtaac acaactgtcc tcgccacgac 61800agcctcaacc ccaggaccct ctgaccgccc actgcaacat gcacctggta cagcaggcgc 61860tgtgggatct gctccggtgc tggccgaagc tctgaccttg ctccggtgct ggccgaagct 61920ctgaccttgg gctgcccagg cccaccagtt atcccgtgaa cactgccagg ctgcaacaac 61980tcatgagcag agcatggggc tccgtccttg ctggtgtctg ggagttaccc ctttgttttt 62040ccaaccaggc atgtcagaaa tgatatctca ttgttaactt tcatttgcat ttctctaact 62100acaggtgaag ttaggagttt ttccatatgc tggttgccca tttacatttc ttcttttgtg 62160aattgcctat tcatattctc tgctcatttt tccactggag tattggtatt ttcccgtcaa 62220tgtaaaggag ctacctgtgt atcatagata ggcatctctt agctgtcact tctgttatgg 62280ttactcaccc taatctctgt tttgcttttt ggctttgttt atggtttatc actcaagttt 62340taaaacatta cataatcaaa tatgcttagc tttcccctta taacttcttg gtttcctttt 62400ccttctaaaa gtcttctgag tgtctgcgta attagttttt ctaaattcgt ttactttttt 62460cattttaatt tttacttcat ctgaattttt tctttttctt ctaagcaact cctctacatg 62520atgagattta tttttgtaaa tggcatgaca gagcagtcca atttaatttt cttctcatgc 62580aaaggcagtg tgatagcatc attaaaataa tatccctttt tcactgagtt gaaaaacctc 62640cattgtgata cactgaattc ctctgtgggg gagtccattt ctggatctta ctctatactg 62700tcccccaatt ctctctgttt tcaagcacag tgtctgctta acagtaacct tacaaatcca 62760gcaaggaaag tgttccttct gtgttttctt ttactttttt tttttttttt aaagataggg 62820tcttactctg ttgcccagtg tggagtgcag tggagcgacc atggctcact gcagcctcaa 62880actcctggac tcaagccatc ctcccacctc agcctcctga gcagttggga ttacaggcat 62940gcgccaccat acctaatttt ttctattaat tttttttttt tttaagacat gaggtctcac 63000ttggttgccc tgcctctcta tgtggtttta atttttttca aaaaattttt gactatgcta 63060gatttctttt tccatatgaa tctttcaatt ttttaattgt tttctctcct ttttagttcc 63120tcttcaccac caccacaccc ccaccaagaa agataaaaaa ttctggctga aattttactt 63180ggaagtgcat taagttttta taatagaatt tggtagaact gacattttta cagtttcctc 63240cccaatatca aatcactgtt aagagttcat tgtatatcct ttaaaataac tttatagttg 63300gccgggtgcg gtagctcacg cctgtaatgc caacactttg ggaggctgag gcaggcaaat 63360cacaaggtca ggagttcgag accagcctgg ccaacatggt gaaatgccgt ctctactaaa 63420aatacaaaaa attagctggg cgtggtggca ggtgcctgta atcccagcta ctcgggaggc 63480tgaggcagga gaattgcttg aacctgggag gtggaggttg cagtgagccg agatcacgcc 63540actgcactct agcctgggtg acagtgcgaa actccatctc aaataaataa ataaataaat 63600aaataaataa ataaataaat aactttatag tttttcctta tatgggcatt atattttatt 63660gttagtatgg agaaaggcta ttgatttttt tgagaaatat atttatctaa acactttgcc 63720aaatgaattt attaatctag tagcttttaa aaaaattaga atctcttgtg ttttctaggt 63780ctaccatagc attaccagaa aagatcatct tccctgttct ttgacaatat gtttattgat 63840tgttttcctg ttttatcatg tggtatgacc tctaaaataa tgttgaataa aaacagtgat 63900atcagcattc ttattatttt aatggaaatg gcattagagt tgcacctttt tgatgttagc 63960tatctttatc atataaagta aggttcctcc tattaatttt tattagttgt ggctattaaa 64020tgttattaaa ttccctttta gtgtgaattg atataatcat aaactttatc cttatgggta 64080taataaatta tggtggtagc tctcttgata ttgaaatatc tggaataaac cctgcttggt 64140tattacatat agtcatttaa tacactgctg cactcacttc gctaatatca cctttagttt 64200tcttttaaat ctttatttaa tacttggatt ggtcttcagc cttcttttga agcctgcctg 64260tatccagttt cagaactaag cttacaccag ctttatatga tcaatttggg gctgataaag 64320agccctggaa atgtcagctg tctagtggct gggtgagact cagctgctcg ctgtgtaggt 64380ctgtttccaa tcactatatg gaaattggcc tattttctac aatttcttct aatttatctt 64440ttcctaggtt tttatccatt tctctaattt ttcaaatttt tgccatatta ctctcattta 64500aaaaatatgt aatcataatt actgttcttt ttttttttta agagacgggg tcttgatatg 64560ttgctcaggc tagactgctg tggctattca caggtgtgat catagcacac tacagcctca 64620agtgagcctc ctgcctcagc ctctcaagta gctgggacta cagttgtgta ccaccatggt 64680gcctggctat aattactctt tttatgattc cttttttatt ccgaatcttg catttttttt 64740ttctatctct ttttttccat cagcctcatg aggaggttat tattattatt ttttggacct 64800ttcaaagaac cagatcttgg atttactatc ctctggcttt catttgcttg agtctttata 64860gtttgcaaag tttccatttt cagaagaaga aagagaccca ggaacattct gtggcttgct 64920caaggtccca cagcctgcta gcaagtggct gaactggaat ttaagctagg cccctgactc 64980caggctgctc caagagatct ctgagccaag cttttgaagg caccatcagt ggtgcctggg 65040atctccgggg atctcccagc aattttgtct tcttttaggt cacctgtccc tttacctgct 65100gcttcagttt cttaggcaca gggcttctct ctggtaacag acaggaagct cctctgaggc 65160tacgtgcccc tgggagactg tggggaggct ggaatctaga gtcagatgac ctggcttgaa 65220tcctagctct gtggctggct gtgggcacct gtgtctcagt tttcttatct gggtaaaatg 65280atcaacatca atagagccca ctacctagga tggttgtggt gatgattaca caaataaaga 65340taggttatct aaaaccccag tggtcaaatg tgcttcagaa tccagaattt ttaggatttt 65400agcagtgggt atacttcatt atgtgccata ccccctccca cccccagtct agggcaacat 65460actattttac aatcgaacag agcagtattt ctgcagtgat ccacatacat atcacattga 65520atggaaaaag cctctaaata acctcaccta ttgcttcaaa gaaggtttgt ttacaaacaa 65580tgaatttgct gcacagtttt gaaatatgca gcagttttca gattttttag atttagaatc 65640gagggtaagg gattgcggac tgtgcaggtt aacccatagg atagccttgg catgtggctg 65700gtgcctgacc tatccagctg ctattgtgtc cacagctccc aaggtccagt tcatggacat 65760tatttgctga atgctcatct gccagtctcc cacggtgtcc tctgcgcagt tcctccctac 65820tcccagggac ctatgcccag tccagtcctg gccttggccc ctgctgtctg cacaccagct 65880cccagcctgg cagcaggctg gtgccaggaa cagggggaga caaagccctg tgcccgcctg 65940cctttcctcc agggtctccc tcttcctcct gcctcatctc cggtggacat aagaactcac 66000atcatcatca aaggtggcat accaggactg gcctggttcc tgaccccaga acccatggtg 66060cccctcagtg gtcttggtct ccagcttagt ccacagcttc acctgcctgg cccctggcag 66120caccttgact gggggccctt ggtcagggtg ctggggtgga gagtgcagaa tatgatccca 66180gggcaggtcc tccacagtgt gcctcgactg gccaggggct tctcaagtgt gccttgggga 66240gtagaaccct ctccgcccct atggacagcc cacctcaggg cctgcaggaa gccctagtgt 66300ctttcaagac atagcttcct cagctggagt gtgaatggag ccacccagga gccctggatc 66360gcagaggact gagctgctca gaaaggtgtg ccccccagca gcctacctgg atggcaatgg 66420ggacaatctt gttggccagg ttcttataca gcaagcagat gggagcggcc aggaactgga 66480gtgtgcaggg gtctgttttg ttggcatcga tgccatccag cagctcaaag tccacgatga 66540aaatgttccc ttgctgcagg aggcagagag atgtgtccaa atcaggctca gcagacggac 66600caaaaatcag gcctggggaa agagtgatga cctctgggca cccctctccc tttcctgcga 66660ctcagcggag ctcagaatgt gtacttctct cctctgactc caaggctgat tgattcacat 66720ggaaggtgtt cacaggttgt ggcagttcgg ggtaagatga aggaatggca gcagctcatt 66780cggcatttgt ggtgtctgaa acctgcttga ctggcacgaa agatgagaga ccgacccgag 66840tgtcagttta ctaagaagaa agaggttagg tgaaatagat gtgacgagtt aaatgccaat 66900cttcaccacg gggctgaagg tccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67020nnnnggggtc ctcagggact gggcctcagc ccgccggtgg ttccacccta ggcttcccca 67080agtcggtcaa gagccgggag cagctgtcgg agtacctgac cgtggtgatc ttcaccgcct 67140ccgcccagca cgccgcggtc aacttcggcc aggtaggcag ggccgggccc gctgggcagg 67200gctcccttct caaggccgct gcctcctccc ccgccccggt tctgcacgcg tactgcaccc 67260tcggacagcc tcggggcctg gcacgggact tgcaggatgg attctgcccg ctcagccaag 67320ggcgctggcc gcggggaaag aggatggacg gactgcaggg cccgctggag ttggggggca 67380cggggaggac ggggcccagg gggcagctgg gcagcagggc ttcgggggtg cccacgcttg 67440ctggcggtcg tctccgcagt acgactggtg ctcctggatc cccaatgcgc ccccaaccat 67500gcgagccccg ccaccgactg ccaagggcgt ggtgaccatt gagcagatcg tggacacgct 67560gcccgaccgc ggccgctcct gctggcatct gggtgcagtg tgggcgctga gccagttcca 67620ggaaaacgag gtgaagctgg gcagggcggg gcacagcccc aggtcacccc aggttaagcg 67680gttcctcagc ctcagggctt tgtgactcgg gccccaaggc tcacttggag caaaggaatc 67740ctgacttcca aggctggaag ggcccagaag gctgcagccg ccaccaggtc ccccggcctc 67800agcctggaca gagctcaggg tgtgcagggc aggagagcac acagcccagg ctttgctcac 67860tgtcaccaga gggtcgtgtg tgacgccccc tccccccagc tattgacaaa gttcttgcac 67920atgtgtttta ccctggtact ccagagggaa tgaccaagag tttcctgggt tccttccgga 67980cacgtgcatc tcatttaacc taacaactga atccggtgtg tcttcgtgga tgcacccgct 68040ttggggtagc tcagtactgc atagaatggc cggaccagtc caacctgctc cagactgctg 68100ggtgtctgag ttgttcccgg ccaatgtgtt ggtccctcac tggacatcct tgtttttgtg 68160gctttgcaca tgtgttcagt atgaccgcac acattcctag cagacaaatt ccttggtcaa 68220agaatattga tactgaattg ccctccacaa actaaattca aaatcccatt atcaatagcc 68280tatgtttctt ggacctttcc agctcagaag ctttgtgatc ttaatccaat acagtatttt 68340tttaaaatga tatctagctc tgccccgcag acatctgtgt gagaatgcaa ataagcacag 68400gaccccaaca gggcagccac cccttcaggc tccctggccc gctttctgcc ttcctgggct 68460ggagaggcca gtgctggccc cagcgcccct gatgggaggt gagagtgctg cgcaggggtg 68520gcccaagaca gcaggatacc atggctgcaa acaccagcag cccccagctt catctgcatc 68580atctcgtaac cacttggcag caggcagtta ttttcccact tatccatgaa gccccagccc 68640tggagccttc cttagagaag caggttggag gcgacgacac ttgccttccc gaggccctct 68700tgtcaggcag cagagggtga atatggggag gtgaatagat gctccctcct tcatctccca 68760aacggtggct ggccccttgg gatgagacag gcctgtcagt ttacacgggt agtggattga 68820cctatgtgtg tgtccatgtc tgggccctca gctgttcctg ggcatgtacc cagaagagca 68880ttttatcgag aagcctgtga aggaagccat ggcccgattc cgcaagaacc tcgaggccat 68940tgtcagcgtg attgctgagc gcaacaagaa gaagcagctg ccatattact acttgtcccc 69000agaccggatt ccgaacagtg tggccatctg agcacactgc cagtctcact gtgggaaggc 69060cagctgcccc agccagatgg actccagcct gcctggcagg ctgtctggcc aggcctcttg 69120gcagtcacat ctcttcctcc gaggccagta cctttccatt tattctttga tcttcaggga 69180actgcataga ttgatcaaag tgtaaacacc atagggaccc attctacaca gagcaggact 69240gcacagcgtc ctgtccacac ccagctcagc atttccacac caagcagcaa cagcaaatca 69300cgaccactga tagatgtcta ttcttgttgg agacatggga tgattatttt ctgttctatt 69360tgtgcttagt ccaattcctt gcacatagta ggtacccaat tcaattacta ttgaatgaat 69420taagaattgg ttgccataaa aataaatcag ttcatttaaa atgggtcttg ttccatgtgt 69480tatattccaa tcaccccaaa cagctcactg ccatctccca caccaagaga aaaaaaaagc 69540catagtcacc gtgattttat caaggcaaaa gcctccccac ccaagtctgg atagaaggtg 69600ctttttccct caagcagtgt ctactctctt tggaacacat cccaaatgtc accacctctg 69660ggaaggcatc cttggttcct gtctctaccc tggatttgtc agaacctctg ttcttgcccc 69720ctctgtggtc tctcccttgg gacaggagcc cctggaagga cagaaaaccc actttattta 69780tgtctgtgcc ccagggctgg cataaggcac cagcactcag ctgttctctc cttttcctcc 69840tggaagggca cctagctaat ggatattagg cattgtcttg ggagctgggg gcttattttt 69900tcctgaggca gggttcttaa catgacataa aaaataaaaa tgggcagggc gcggtggctc 69960acgcctgtaa tcccagcatt ttgggaggcc aaggtgggag gatcatttga ggtcagaatt 70020caagaccagc ctggccaaca cggtgaaacc ccatctctac tgaaaataca aaaattagcc 70080aggcatggta gcaggtacct gtaatcccag ctactcagga ggctgaggca ggaaaattgc 70140atgaacctgg gagacagagg ttgcagagag tggatatcgc accactgcac tccagcctgg 70200gcaatagagc gagactctgt ctcaaatagt taataaataa ataaattaaa ttaaataaaa 70260acgcattctc tttccccagc cccaagtagc agagcccgct ctgagcctgg gcatggtgac 70320ccattctcct cgtgctcaca tgcccaactc ccagccctcc agcccctggc ttcggaatca 70380tgtcatcagg gtctcccatg tctctggaag gtgcttccag tgtggcaggc catcagctca 70440gtgtctgggt aaaggcttct acagggccag catggcatag gggggaggtg tgcatgcgtt 70500cctgaagcca caaggtcttc tgaggagacc tgactattgg ggtggggact agagactgag 70560gtccagcccc ttaatgcccc agactccctg ccatccctcg gagaatttcc caccacactt 70620atgaatgatc ttcgggacag gtgacataaa catacagcta tggtagaggc attcagctcc 70680atgccactgt gagaatgaac ataccaactt ttaggcaaca aggccatgtg gtcaaggaga 70740gaggagaaac gaataggagt aggcaggggt ggtgaagcag gtctgacatt ggcaaaagtg 70800acagagaagg attgggttgg aagggcttca gatggcagag aacctctgaa aaagtctcat 70860gccagctaat gaagagtcct caaagtcacc atcacagaaa tcttacatct ggcagaaatg 70920acagcaatag tttggcatga accccacagt ggatccaaac gtgtggcagc tgagatcgtc 70980agttaattat gttctctgca gctggtcctt ctggttccct ggagctgggt atctgagtag 71040cacagctcca ctgctaccac aggagaggga gaatcccaaa ttctgtgcat agactgtcta 71100aacctctgga tgacccctaa gacacatgta cataaggcaa actgcaaaca gcccaattta 71160ggataaaaca gcctgtctta ctctgtttgt gctgctataa taaaatacct cagactgggt 71220catttatata gaacagaaat tgattcctca cagttctgga ggctggaagt ctaagaggag 71280gcactggcag tttcagtgtc tggtaaggga gggatcagtg tctgctccca agaggatgcc 71340ctctgtgctg tgtcctcacc cagcagaaga gcaaaaaagg gcgaacattc tcaaaagcct 71400cttttataag gacatcaatc cattcatgag ggcagaaccc tcaagaatta atcatttctc 71460aaaaggcccc tcctcctaat gccatcacct tggggtttaa gttccaacac atgatttttg 71520gaaggacaca tacattcaaa tcattgcaca accaaagtga tatttgaact ttcaaatagt 71580ttgcagtttg agatcaacta agttaattgc ttgcttgaat aaaaacatca cagccgggca 71640cgatggctca cgcctgtaat tccagcactt tgggaagcca aggtgggcag atcacttgag 71700gtcaggagtt cgaaaccagc ctgaccaaca tggtgaaacc ccatttctac taaaaataca 71760aaaattagcc aggcggccgg gcgtggtggc tcatgcctgt aatcccagct ctttgggagg 71820ccgaggcggg tggatcacaa ggtcaggaga ttgagaccat cctggccaac acagtgaaac 71880cctgtctcta ctaaaaatgc aaaaattagc tgttgagact actcgggaag ctgaggcagg 71940agaattgctt gagccgagat cgtgccatcg cactccagcc tgggggacaa gagcaagact 72000tcgtctcaaa aaaaaaaaaa gaaaagaaat tttctggatt aggataatgt tctatatttg 72060gataagggtt taggttacct agatagatac atttatcaaa agtcatcaaa tggtacactt 72120cagatgtacg catgtcactg tatgtaaata ttaccccaaa atcaacaaga accatgagca 72180aatactgact tctagtgaac gacatgcata ctgaaacttt cgtagtgaag tataattatg 72240gctgcagttg actctgaaat acataaaaac ctaagttgat gggtgaatac agtgattaat 72300agatagaaat ctgtggtaaa gcatacggag aaaaatgtta attgtagatt ctagggttta 72360tatatattct cactatataa ttaaacgttt ttatgtttaa aaatttttat aatgttgaaa 72420aaccatgaaa tcctacctaa cgcagtaaga caagaaaaag agataaaagg tatatgtact 72480gggaagtaag aaataaaacc gtctttgtta gcagatgaca tgctcatcta tgtagaaaat 72540ccaaaagaat ggataaagaa actcctggaa ctaataagtg gttacagtaa ggttgtagga 72600tacaaggtta atatacaaaa gtcaatcact ctcttattca ccagtaatga acaagtggaa 72660attgaaattc aaaacaaaat actatttaca ttaatactcc aaaaatacat acttaggttt 72720aaatctaata aaatacatac aagatcttta tgaggaaaac tgcaaccctg atgacagaaa 72780tcaaagagaa actaaagaaa tagacataag taaatattcc ccagaaagac tcaatattgt 72840caagatgtca gttcttcaaa acttgaccta tagattcaat gcaatcctaa tcaaaatctc 72900agcaagttat gttgtggata ctgacaaact tattcttttt tttttttttt ttttttttga 72960gacggagttt cgctctgttg cccaggctgg agtgcagtgg cgcgatctcg actcactgca 73020agctccgcct cccgggttca cgccattctc ctgcctcagc ctcccatgta gctgggacta 73080caggcacgcg ccaccatgcc cggctaattt ttgtattttt agtagagacg gggtttcacc 73140gtgttagcca ggatggtctc gatctcctga cctcgtgatc cacccgtctc ggcctcccaa 73200agtgctggga ttacaggcgt gagccaccgc gcccggccaa cttattctaa aatttagata 73260gggaagcaaa agacccagaa cagctaacac aacaatatag taggaaaaca acaaatttgg 73320aaaatggata ctacccaact ccaagactta ctataaagct acagtaatca agatagtgcc 73380gtattggtga aagaatagac aaatagatca atggaacaga atagagagcc taagaaatag 73440accttcataa atacagtcaa ctcatccttg acaaagtagc atagacacta caatggagca 73500aagattgtct tttcaacaag tggtgcagga acaactggat atccacatgc aaaaacaaac 73560aaacaagcat gaatctagat acagacctta tgctcttcac aaaaattaac tcaaaatgga 73620ttatagatct aaatgtaaaa ttcaaaacta taaaattccc ataacatagg ataaaatcta 73680gatgacctag gtatggctat gactttttag acataacacc aaagccatgg tccatgaaag 73740aaataattgg taagttggac ttaattaaaa ttaaaacctt ccgctctgca aaagaccaca 73800ctaggagagg gaggaaatat ttacaaagac acatctgata aagaattgtt acttaaatta 73860tacaaagcct tcttaaactt aacagtaagg aaacaatctg attgaaaaat gggccaaaga 73920ctttaacaaa caaaagaaga tatacaaatg gcaagtaagt atgtgaaaag aagctgcaca 73980gctggggggc gtggtggctc acgcctttaa tcccagcact ttgggaggtc aaggcgggca 74040gatcacctga ggtcaagaga ccagcctgtc caacatggaa aaacctgtct ctactaaaaa 74100tataaaatta gctaggcatg gtgatgcatg cctgtcatcc cagctacttg ggaagctgag 74160gcaggagaat cgcttgaacc cggaaggtgg aggttgcagt gagccaagat cgcgccattg 74220cactccagcc tgggcaacaa gagtaaaact ctgtctcaag aaaaaaaaaa aaaagctgca 74280cattctatgt tatcagggaa atgcaaattt agaataacgc aataacaaga taccactaca 74340tacatattag aatggccaaa attgagaaca ctgacagcac caaatgtgga ggaggatgtg 74400gagcaacagg aactagagag aatgcaaaat ggtatagcca cttaggaaga cagcttggca 74460gtttcttaca aaactaaata tactcttacc acacaatcca gcaatcacac tccttggtat 74520ttacttacat cccaacaaaa accctcatat ggatgtctag agcagctttg ttcataattg 74580tcaaaacttg gaagcaacca aagatgccct tcggtaggtg agtaaataaa taatatccag 74640acaatggaaa taccattcag tactaaagag aaatgagcta tcaagccatg aaaaggcgtg 74700gaagaaactg aagtgtgtat cactaaaagt caatctgaaa agggtgcata agtttccaaa 74760gatatgatat tgcagaaaag gcaaaactgt ggagacagta aaaagattgc caggggctag 74820ggcagtggga gggatgaata ggcagggcac agaggatttt cagggcaata acactactct 74880gtatgacact ataatgttgg atacatggcc agatccacag aatgtagaac accaagagtg 74940aaccttgaac tatggacttt gagtgatgag gatgagtcag tgtaggctca tggattgtaa 75000taaatgcacc actgtggggg gggggggata ttgataactg gggggctgta catgtgtagg 75060gacaggagta aatgggaaat ctctactttt gttaccggtg gagggtgtac cgcattttga 75120acaaaaaatt ggacaaaacg cacaaagcaa ggaaagaatg aagcaacaaa agcagagatt 75180tattgaaaac gaaagtacac tccacagggt gggagcaggc ctaagcaggt ggctcaaggg 75240cctgaataca gatttttctg gggtttaaat accctctaga ggtttccatt ggttacttgg 75300tgtataccct acgcaaatga agaggatgaa gggaggttac aaagttattt acttggtgta 75360gaaaattagg gtttttccct tttatttagt tgtaggaagc ccttaggtta cctgcctcca 75420gaccctattc tcctgcctca ttttattcct aattttgttg tgaacctaaa attgctctaa 75480aaaatgaaat cttttaaaca agaaaataaa cttataaatt gcaaatatgt gtcacttaca 75540taaaaacaaa gaattagtgt ccctacatca ggaaaaaaac acctataatt caatgtaaaa 75600atgcaaaaaa gccaagagaa atggccaagc catggaaatt cacagaagta gaaataggca 75660gtaaataaac aatgctcaat ttcatttatc tgagaaatgg agagtaaaac ttcagtgaca 75720tacaaaggag taacaatcat tttaggaaca attttaaaaa taacacttgt gatgtggctc 75780aggaaagatt catattctca ctgtaggaag gtaagtgaat cattttagag gtcagttttg 75840caacttagaa gatcaaatgc atctaccctt tttctacttc tagaaatgta tcttcagaag 75900tgcccctccc tccctcacac acccccaagt gtacaaaaat aaatgacatg gatgtggact 75960gcagccttgt ttctaatacc aaaaatcagt aaacaaccta aaaatctgta tccatgagga 76020tttaaagaaa tcctgcatga gttagaaaga ataaggtaga tgcctgggtt ctaacataga 76080aaatatcaaa ctggttgtta gatgaaaaaa gcaagttgca aagtgaggta atgtgtaaat 76140gcagtaagca aaatacatca tatatatagt actttctatg tatttaccta tatgagcgtg 76200atgcctccaa ctgcatagct gtggctccgg gagcaggacc ttgtaacaga gcctctgccc 76260ctgactgtcc tgaggggcct ttccctggga aaggaaccag gtttgatttt cagtctcttt 76320tgttatcacc atgctgacag ctttgcttgt catatgcctt tccctccgct gtactgattt 76380taccttacag aagctctcct tagtgcttca tcagacactc cctcttttcc aaagaaaaga 76440cagccgcatt ccatggtcat ctggttgagc tcgaggtgag ccccagggat catgtgcctc 76500ccaccaggct gacaccccag tggtgaggag ttgccccttg gctttttcga ggccaagcaa 76560ctcagaggtg tggcaggatt gcatgtcaca gtggcacttc caaggccctg gagagtgact 76620caacgcctct gttattaaga tgtcacacag gctgtgcagc aacaactcta gggacaccct 76680tcacactgac agagttggca agcttttcct ataaaggggc acacagtgaa tactgtaggc 76740tttgtaggcc atactatctg tcccaacaat tcaactttgc ccactgtagc tgtgaatcaa 76800ccgtagataa tattaagtga atgtgcactg ctgtttgcta atagtttatt gacaaaaaca 76860gctgcttttg agcagtttgc taccccgaca gagactgtgg ggagtccctg gtggcagcga 76920gctacaggca gccagtaccc catcagtgtg gctggttcca tcctctcaag cctgcctgca 76980cccagacact gtcgagtgtg tcttcagaaa aactcctaaa attccccaaa agcacagtgg 77040ctccccgaga gccacctccc tcaccttctt acaggggcag gttcctcttc cggttccacc 77100ctcctcagca cggagctcag gggccatccc cacctcctct tggccgcaag cacagacctt 77160cccacacagg ctcagcagct ctgcgggcct gtcccccacc tggagctgca ggcctccact 77220cctggggaca gctgtgacca tggctctgca aagcagcctt gtgcctagct cccaagtcag 77280ctggcagccc agagtggaac aattctgtgg atcacctctg agacagcagc acatctgggc 77340aagcctggct tcccacccct cacctcctgc aacctctgac ccttgccatg catgtggtag 77400ggaagctgca accaggcctc agcactccca gacctgtggg cggtttgctg tctgaggatc 77460gtggcactgc cttccctcag aggccttgcc agagccaggg gatgcagcgt ggcagagctg 77520tcactggcaa ccctgctggt ctccctggtg ctttctcagc tgggctgtgg gtcaggacaa 77580atttctatgg ataaggctgg atcataatct agtgctccat ggtcatgcag cgacagactc 77640gagtctgcct tctctcaggg tggtgagctc aggtaggaac gggatgtttg aagcaagcac 77700acctgtgaga aatgcctgtt ttcactccca tcccacacac tcgtccagac actgccagcc 77760cacccccgac acacgtgtct gagggaggct gaggaaaaca gacacctcca ggagccgcat 77820actatttcct gatgtgtaag ccagacaacc ctggaaagga ggtcagggaa ccggagtgcc 77880tgccaacgtc cccagcctcc ctgcagcaaa acactctcat ggcaaaactc ctttttaaag 77940aaaggcttta ttgctgcaat tagacatccc agtgtatagc acaaaccccc ctgtacagag 78000aattattcaa gtggtaatac tgagaaacag tgaacacaca caaaagaata caaaactaga 78060catttagatc actagaaact ttctaaggta agaaaaattt caaatgtgaa gtgcctttta 78120gaaactacac cacacatgcc agtgtaaatt tggtttaaca atcaatttct ataaacattg 78180catctaactg tgcactgatc aactgcacaa cttcaacaat tacttccaag acaaagataa 78240atgcttttat ttccctatgt tttggtcttt ccttgggcta gagacagaag ctgcatgttt 78300caaaccagat tcaaaaggga aagcacttca aaacagaaag tacttcaatt gtgtatttgc 78360cttcagccac atccagagac ttgtggatct cacccggttg gtcccagccc ttctccaacc 78420ctgaagccca cagcaggggt gcagaggcag ccccagatcg tgcagacatc acccgaggct 78480cccaaactgg cccagctgcc tgaggcctgc atgcctgtgg ccaggtagga cacatgtgca 78540ggtttgaaaa ctaggcctac aagactgaag agtggactga agcaaaaagg ggagaaacat 78600tagaggccta cagtaggaag agacctttta tagagttttc aggaaattct cacaataaac 78660ttgggcacaa agaccaaatt gctgaggcca gaagcctgct tatggtactg tgacttatgg 78720ggagtagagg ccaaatcaga gagggctggc tggtgatacc ccagaccact tgcctcttcc 78780ccttccctgg cctacaaata ccaggcctcc tggccctgag acctcattct gggtttctat 78840atgggtcaac caaatgccat cctttcacta aacccaggtg caaaatgggg ggggaagggg 78900ggaagctggg gagtacacag cacccaaaag aaccgagaga gcaagttttc caaggctaca 78960ggaacattgg cagagctggt ggccacacca gatgggagta ccacatcccc accaggcttc 79020atgcttcact gctagatgca gggggctaga agggtctgat gaatcaggag ctgaactaga 79080accttcaaga aaagaaaagg caagggggtg gctctgtgcc ccgccaggag aggaaagaag 79140aatgtgtgtg gattgtccac agtgaggcca gaaacgatga gatggtgtta ggcagaaaag 79200aagggagagg gcttcccatg agaatctggc tgggcatggg agggatacag ggctcagtta 79260tcaaggcacc tttgccatag tggggtgcca gctacattgg gtgggatgcc caggggctgt 79320ccctacctaa gcactttctt gtctacagga agtcttggtg cttttcaagt tcagcataag 79380gggttccata tgtgaagtgg ggattcacac ttagaaaatc tatcctggtc ttcacctgaa 79440acactcatct actgctgggt ggcaggaaca agtatacaaa accaggggcc ttgcctgggc 79500agttcctaag ggacattggc agggctgacc tgggattcag ctcccctgag tggacagtct 79560gtggttgcct ggggtcccca ggctgagaag gggtatgggt aattgcactt cggggcacag 79620tgaacacagg cacgggctgg atgtgccctg ggagacagaa aggagaggct gacctattct 79680caggagctgg ctatggctgg acagtgcctg cccggccaga ggaaggccac agcagagcca 79740ctccacagac taagacctgt gttgccaagg aatcagacac tggcttggag gagacaggag 79800gacaaagcct gctgctatta agcccacacc gtagcttaga gatccttcag cctcctggcc 79860ttcaaaacaa actccagggc ttccactctg aaaggcaaag gaatcacctc gtgtttgctt 79920catcccctta gcaggatgaa cgaaacgcct aaggcccaaa aagactgagg gcaggacccc 79980cagcatctcc catctcctcc gtgcaggctg ggtgagagaa tggcctggaa aggaagcatc 80040tgcgctacat tgtgcttcag gcccccccag caactttccc ggaggtaact atttgcctgg 80100aaaactagga aactgggttt taaaaacaga attttaagca gactttttag agggacaagg 80160ccatttgatg gtagtgattt tggttttgtt ttatgaaaat ggtacaggtg gtaatccctg 80220atgacagaat gcactgagag tggggccaag ggccctgaag gatcctgtct gttcagtctg 80280gttaaggcga cagctgggcg ggcatgtggc cggggagcag tggcacagcg gaccctggca 80340gtttgctcac acctggccct cgtcccagag ccagcccacc tgcctcttgg ccctggtagt 80400gactccacag aacttccccg agcagaagcc tcacagtggg ccagcaccca tcttgcatgc 80460aagggagaac ctgcatgcag tgcacacacc ccggccaggg tggcacatgg ctgctctttc 80520aatgtgaagc caacaccaac tggagagatt taactataaa ggaatatttt ttaaatattc 80580agttttgttg tcattactca cttcctttcc ttccatttcg atctcagaaa ctgttctttt 80640tcagtgttga caaaaaagaa atgtatgggc caggtctaca aagtaacacc ttgtcataaa 80700acaggctcaa aagcatagga atcatgcttt aattgccatt tagcatccac taattcccat 80760gagatctgtg attaaaaaaa aagcctctgg cctgcttgtc ctgtcttaaa agtcatgagt 80820ctgttttatg aacagaaaga atgccacgct tcttagcata tgtaaggcaa aatggatttt 80880tgatctctca tgatgtaaag gagatacaaa ggtaaggaag ggaagatttc taagtgagac 80940tttttttgag tgtcaatccc gctggacaca catattacaa aataaagatt ttcttctgta 81000aagtgctgtt tgctcagtga atcatgcgcc accacagagc accatggctc cacctgctca 81060agagcatgga ggaggcagca tcggcagagg gcaggcagga gtctgtgttt gggggtctgt 81120ttcaatacca tctcctgggg ttcgccgtga tgcagaggga atcttctcgt catggctaga 81180cactccccaa tgctctgctc caggctgggg accactgcaa gctggaggcg gctgggtatc 81240agggcctggg cacccctgct cctcctcttc ccttgggatt ggcattttat tctctcattc 81300agctcaagac catgggcagg aactctgctg gctccccatg atgtcatcat ggggtcttcc 81360actttcccac agagctgcca ggcagaggca ggacccaggc atgcctggcc cacaggaagg 81420ttttctagga gactaaggag ggtttagaaa aagagaagcc actataaata gtcacctgtc 81480cagtctatgc tattaaagga cataagaaag gtatacaatt ggcagtaaac aatttccttc 81540agctcttcat ggatgtccag gaaaatgaca acccgcacac aatcagacgt gaatgatttc 81600tgctccagtg tcttcaggct ctgtgcaaca agaagagttt gtgtcggaat gacagattcc 81660atatccatgt ttattttcaa agttgggctc tgttagtgga gatttttcaa aaatattctt 81720tttgcttgtt tctggacagt tttgaacata gatcactcta ttataggcct tgagtctctt 81780ccacaattgc acatacactt tacactgaac atacataaaa agaagtcctc cggtgaagcc 81840gatggccaca accaccaatt tagtccaaaa gggccattct aggattcctg gaagggaaaa 81900actcagccta ttagatttta tttaagatat gtcttcacca cggggctgaa gnnnnnnnnn 81960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 82020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcggtgac tgtgacattg aggttcggcg 82080tcgggggaga gggaaaagga gagggagagg gagagggaga gggagaggga gagggagagg 82140gagagccata tgtagtttaa agtagttttt ttccaattct gagaagaagg tcattggtag 82200cttgatgggg atggcaatga ctctataaat taccttgggc agtatggcta ttttcacgat 82260attgattctt cctacccatg agcatggaat gttcttccat ttgtttgtat cctcttttat 82320ttccttgagc agtggtttat atttctcctt gaagaggtcc ttcacatccc ttgtaagttg 82380gattcctagg tattttattc tctttgaagc aattgtgaat gggagttcac tcatgatttg 82440gctctctgtt tgtctgttat tggtgtataa gaatgcttgt gatttttaca cattgatttt 82500gtatactgag actctgctga agttgcctat cagcttaagg agattttggg ctgagacgat 82560ggggttttct aggtatacaa tcatgtcatc tgcagacagg gacaatttga cttcctcttt 82620tcctaatcga atgcccttta tttccttctc ttgcctgatt gccctggcta gaacttccaa 82680cactatgttg aataggagtg gtgagagagg gcatccctgt cttgtgccag ttttcaaagg 82740gaatgcttcc agtttttgcc catgatgtat gatattggct gtgggtttgt catagatagc 82800tcttattatt ttgagataca tctcatcaat acctaattta ttgagagttt ttagcatgaa 82860gcgttgctga attttgtcaa aggccttttc tgcatctatt gaaataatca tgtggttttt 82920gtcattggtt ctgtttgtat actgggttac atttattgat ttgtgtacgt tgaaccagcc 82980cttgcatccc agggatgaag cccacttgat catggtggat aagctttttg atgtgctgct 83040ggattcgttt gccagtattt attgaggatt tttgcatcga tgttcatcaa ggatattggt 83100ctaaaatggg ttttttgttg ggtctctgcc cagctttggt ctcaggatga tgcctcccct 83160attaaaagag ggagggagga ttccctcttt ttctattgat tggaatagtt tcagaaggaa 83220tggtaccagc tcctccttgt acctctggta gaattcggct gtgaatcctt caggtcctgg 83280actgtttttg gttggtaagg tattaattgt tgcctcaatt tcagagcctg ttattggtct 83340attcagagat tcatcttctt cctggtttag tcttgggagg gtgtatgtat ggaggaattt 83400ttccatttct tctagatttt ctagtttatt tgcatagagg tgtttataat attctctgat 83460ggtagtttat atttctgtgg gatcagtggc gatatcccct ttatcatttt ttattgcatc 83520tatttgattc ttctgtcttt tcttctttat tagtcttgct agcggtctat caattttgtt 83580gatcttttca aaaaaccagc tcctggattc attgattttc tgaagggttt tttgtgtctc 83640tatttccttc agttctgctc tgatcttagt tatttcttgc cttctgctag cttttgaatg 83700tgtttgctct tgcttctcta tttcttttca ttgtgatgtt agggtgtcaa ttttagatct 83760ttcctgcttt ctcatgcggg catttagtgc tataaatttc cttctacata ttgctttgaa 83820tatgtcccag agattctggt atgttgtgtg tttgttctca ttggtttcaa agaatatctt 83880tgtttctgcc ttcatttcat tatgtaccca gtagtcattc aggagcaggt tgttcagttt 83940ccatgtagtt gagtggtttt gagtgagttt cttagtcctg agttctagtt tgattgcact 84000gtggtctgag agatagtttg ttacaatttc tgttctttta catttgctga ggagagcttt 84060acttccaact atgtggtcaa ttttggaata ggtgtggtgt ggtgctaaaa aaaatgtata 84120ttctgttgat ttggggtgga gagttctgta gatgtctatt aggtccgctt ggtgcagagc 84180tgagttcaat tcctgggtat ccttgttaac tttctgtctc gttgatctgt ctaatgttga 84240cagtggggtg ttaaagtctc ccattattat tgtgtgggtg tctaattctc tttgtagatc 84300tctaaggact tgctttatga atctgggtgc ccctgtactg ggtgcatgta tgtttagtat 84360agttagctct tcttgttgaa ttgatccctt taccattgtg taatggcctt ctttgtctct 84420tttgatcttt gttggtttaa agtctgtttt atcagagact aggattgcaa cccctgcctt 84480tgttttccat ttgcttggta gatcttcctc catcccttta ttttgaacct atgtgtgtct 84540ctgcatgtga gatgggtttc ctgaatacag cacactgatg ggtcttgact ctttatccag 84600ttttccagtc tgtgtctttt aactggaaca tttagcccat ttacttttaa ggttaatatt 84660gttatgtgtg aatttgatcc tgtcattatg atgttagctg gttattttgc tagttagttg 84720atgcagtttc ttcctagcat cgatggtctt tacaatgtgg catgtttttg tggtggctgg 84780tatgggttgc ccctttccat gtttagtgct tccttcagga gctcttttag ggcaggcctg 84840gtggtgacaa aatctctcag catttgcttg tctgtaaagg attttatgtc tccttcattt 84900atgaagctta gtttggttgg atatgaaatt ctgggttgaa aattcttttc tttaagaatg 84960ttgaatattg gccctcactc tcttctggct tgtagagttt ctgccaagac atgagctgtt 85020agtctgatgg gcttcccttt gtgggtaacc tgacctttct ctctggctgc tctcaacatt 85080ttttccttga tttcaacttt ggtgaatctg acaattatgt gtcttggagt tgctcctctc 85140gaggagtatc tttgtggcat tctctgtagt tcctgaattt gaatgttggg ctgccttgct 85200agattgggga agttctcctg gataatatcc tgcagagtgt tttccaactt ggttccattc 85260tccccgtctc tttcaggtac accaattaga tgtagatttg gtcttttcac atagtcccat 85320gtttcttgga ggctttgttc attttttttt tcttttttct ctaaacttct cttcatgctt 85380catttcattg atttgatttt ccatccccaa taccctttct tccagttgat tgaatcagct 85440actgaggctt gtgcattcat catgtagttc tcatgccatg gttttctgct ccatcaggtc 85500atttaaggac ttctctgcat tggttattct agttagccat tcatctaatt ttttttcaag 85560gtttttaact gctttgccat gggttcggac ttcctccttt agctcagagt agtttgatca 85620tctgaagcct tcttctctca actcgtcaaa gtcattctcc atccaggttt gttctgttgc 85680tggtgaggag ccgtgttcct ttggaggagg agaggcactc tgatttttag agcttccagt 85740ttttctgctc tgtttgttcc tcatctttgt ggttttatct acctttggtc tttgatgatg 85800gtgacgtaca gatggggttt tggtgtggat gtcctttctg tttgttagtt ttccttctag 85860aattcaggac cctcagctgc aggtctgttg gagtttactg gaggtccact ccagaccctg 85920tttgcctggg tatcagcagc agaggctgca gaacagtgga tattggtgaa cagcaaatgt 85980tgctgcctga tcattcctct ggaagttttg tctgagagga gtacccagcc gtgtgaggtg 86040tcagtctgcc cctactaggg ggtgcctccc agttaggcta ttcgggggtc agggacccac 86100ttgaggaggc agtctgccca ttctcagatg tcaagctgtg tgctgggaga accacttctt 86160tcttcaaagc tgtcagacag ggacatttaa gtctgcagtg gttattgctg tcttttgttt 86220gtctgtgccc tgcccctgga ggtggagtgt acagaggcat gcaggcctcc ttgagctgtg 86280gtgggctcca gccagttcaa gcttcccggc tgctttgttt acctactcaa gcctgggcaa 86340tggcgggcac ccctccccca gcctcgctgc cgccttacag tttggtctca gactgctgtg 86400ctagcaaaga gtgaggctct gtgggtgtag aaccctccaa gctatgtgtg ggatataatc 86460tcctggtgtg ctgtttatta agcccattgg aaaagcacaa tattagggca ggagtgaccc 86520aattttccag gtgccatctg tgaatgcttt ctttgactag gaaagggaat tccctgacct 86580cttgcccttc ccaggtgagg tgatccctca ccctgctttg ggtcacgcat ggtgtgctac 86640acccactgtc ctgcaaccac tgtctggcac tccccagtta gatgaacccg gtacctcagt 86700tggaaatgtg gaaatcacct gtcttttgca tcgctcatgc tgggagctgt agactggaac 86760tgttcctatt cagccatctt ggctccacgt atatccactg ccacacttct ttagtcataa 86820agtgagtgcc ttggtcagag gcaatgctgt gtggaatacc atgatggtgg ataaggcatt 86880ctgtgagtcc atgaatggta gtcttggcag agcattgtgt tcagtttagg caaacccata 86940tccagagtaa gtgtctgttt cagtgaggac aaacctctgc ccttttcagg atggaagagg 87000tccagtataa ccaaccttcc accaggtaac agactgatca ccccgaggaa tggtgccata 87060ttgagggctc agtgttagtc tctgctgctg tcaaattggg cactcagctg tagccatagc 87120caggccagtt ttggtgagtg gaagtccatg ttgctgagcc catgtataac ctccatccct 87180gccaccatgg ccactttgtt tatgggccca ttgggtgata acaggggtgg ctggggaaag 87240aggctgagtg atgtccacag aacaggtcat cttatccact tgattattaa aatcctcctc 87300tgctgagatc acccattggt gagcactcac atgggatcca aatatcttca caggttttgg 87360ccactcagag aggtccatcc acatacctct tccccaaatt tctttgtcac caattttcca 87420atcatgcttc ttccaagtcc ctgaccatcc agccaaacca ttggctacag cccatgaatc 87480aatatataat cgcacatctg gccatttatc cttccatgaa aagtgcacaa ccaggtgcac 87540tgcttgaagt tctgcccact gggaagattt cccttcacca ctgcccttca gtgatgtcct 87600agaaaggggc tgtagtgctg cagctgtcca cttttgggtg gtgcctgcat atcgtgcaga 87660accatctgtg aaccaggccc tagtcttctc ttcctctgtc aactgatcat agagaactcc 87720ccatgaggcc atcggtgcag gctggggaag agaaggtggg gtggcagttg tggagaccat 87780gggcatttga gctacttcct tgtgtaactt acttgtgcct ttaggacctg ctcgagccca 87840atcacatata tatcacttcc atttgatgat ggaatgctgc tgtgcatgac ccattttatg 87900gctacatggg tcagaaagca cccagttcat gatagacagt tcagttcaca tggtgacttg 87960atgacccata atcaaatgtt cagtttccac caaagctcag taacaggcca agagctgtct 88020ttcaaaagca cagtagttat atgcagaaaa tggcagggcc ttgtttcaaa attttagagg 88080catctgctgt gattcacctg tgggggcctg ccaaaggctc caaacagcat ccctatctgc 88140cactgatgcc tcaagcacca ttggatctgc tgagtcatat ggcccaaaca gcagagcagg 88200ttggacagca gcctggacct gaggcagagc cttatcctgt tctggacccc actcaaaact 88260ggcagccttt tgagtcactc gataaatggg cctgagtaac acacccaaat gaggaaagtg 88320ttacctccaa aatccaagta ggcccactac atattgtgcc tttttcttag ttgtaggagg 88380ggccaaatgc agcaacttat ccttcatctt acaaggagta tcttgacagg ccccacacca 88440gtggacccct agaaatgtta ctgaggtaga aggtccctga attttagtca aatttatttc 88500tcatcctctg gcacacaaac atctcacaaa taagtccagt gtgtttgcta cttcttgctc 88560actggatccg atcagcataa tgtcatcaat gtaatggacc agtgtgatat cttgcagaag 88620caaaaagtgg tcaagatctc tccaaataag attatgatgc aaagccagag tttatatact 88680cttgaggtag gagagtaaag atatattgct agtcttgcca gctgaaggca aattgcttct 88740agtgggcctt atgaatagga atggagaaaa aggcatttgc caagtcaatg gctgcatacc 88800aggtaccagg aaatgtgtta atttgctcaa gcaatgaaac cgtatctggt acagcagctg 88860caattggagt caccacttag ttaagcttat gataatccac tgtcattctc caagatccat 88920ctgtcttatg cacatgccaa atgggagagt tgaatggggg tgtgatgaaa atcaccaccc 88980ttgcgccttt caagtccttg atcatggcac taatctctgc aatccctcca gggatgtgat 89040attgtttttg atttaccatt tttctaggta gaggcagctc taatgccttc catttggcct 89100ttcccaccat aataggcctc accctaccag tcagggagcc aatgtgggtg ttcttccagc 89160tgctaagtat gtctatgcca attatgcatt ctggcactgg ggaaatgaac acaggatgag 89220cctggggacc cattggaccc actgtaagtc agacctgagc taaaactcca ttaattacct 89280cacctccata agctcctgct ttaactggag gaccacaatg acgttttgag tcccttagaa 89340ttaacgtcag ctcagagcta gtgtcctgta atcccccaaa tgactgataa tttccctttc 89400cccaatgcac agttaccttg ataaaaggct ggaggtctcc ttgggaaaaa atgggagaaa 89460gattaacagc ataaattgtt ggtagtgtag tgggctcctt ccttaatggg acccaacttc 89520ccctttattc aaggggttct gtgtctgtaa actggctcaa gtcaaaaatt gattgagggg 89580ccatgattct ctatttttat aattcaaagt agtgttttgt ccatttgacc tggaagtttt 89640ctgcttatat aaattaagta ggaaagaagt aggcatccta acaatttcac ttctgggaac 89700actgtgatta attagcgaat gccagaactc tacatgagtc agactattct gattgctgct 89760ttgccactac tgtctgttat ggtagttatg cccaccttgc ctttgatggt tgagtgccac 89820cacttggcct ctgccacctt gggatccaat tattcccatt gtatttacct ttattagttg 89880agtgactgtg gttctcacca ttggatctga tatgcagaga attacggggc tcttcaaaga 89940tgcaggtgct accctcacag atctattttg caaggtattg gtcaagggta tatcttctgg 90000gcccttccat ctgggatgag taggtctaaa gtgactaatc cactccacca tctcaatctc 90060tctaagcctt tggatccctt cctctacatt aaaccaagag tgatcagaca tttccagctc 90120cctcacagtg gccatctttc aatccatatt tcagctaact aagcaaataa attattagaa 90180ccttttttta actccctgag ctgcaatatt aaatgcagag tctctactta gtgggcccaa 90240atcaacaaat tcagcctgat ccaactctat gttccttcca ccattatccc acacccttaa 90300tatccattct catgcctgtt ctccagattt ctgtttatat aaattagaaa actcaagtag 90360ttcttttcaa gtatagtgaa cctcctcatg ggtttcactc tcaacctcac ctcttggggc 90420ccataaggac tttagtctgg ttataggtct agaagcagac aggggtgttg ggggtggctc 90480ctgaggagaa tcaacattat tttgcctggc aactgctcag ggggggccat cactgttgcc 90540ttaggcagca cagtgtttat ctccccagac aaatatggaa aggctgatgg cagcatgggt 90600cagggagagg atgtcaccac tactggggat ggggaagctg ttttttttct ggcaaaaaag 90660gtttcctgag actttacaaa ctcagtgtcc ccagcttcat cagggtcctc ccacatgtcc 90720cctttccaag ttgcagggtc ccattctttt ccaaacgatg ccctcacttt aatagtacat 90780acctggtgag gctgtgcatg cacccttcat tggaagtcag ccacttgcac aataagagct 90840catatccatt ttcccacaat ttcagctctt tctctgcagg agataatact ttcactcagg 90900gcaatcttag cagatttgag gctcagtatc tgcttctgaa gccaggagtt agaatcccta 90960agttcatttt ctttcatcac tttgtccatt gaacttagga gaaaccaacc aacttcatta 91020tgtttccaca tatggtcaaa ggtattattt atagagtcac taaactcctt gcctctcatg 91080agcgctgaat caggaatgtc aaatgcattt attttgctta actctctaaa cagttcatag 91140aaaggaacac tgaatcagtg ttctccatac tatcagaagt agagtcctta gcatttttga 91200gtctaatcat attaaacagc cactccagaa accctaaaac caatgaaaga actgcatcct 91260taatgttctg ttcctctaga accactcctg gtacaaaaat ctgtgttagg gttctctaga 91320ggggcagaac taataggata gagatatata agaaggaaag tttattaagt attaactcac 91380atgatcacaa ggtcccacaa taggatatct gcaagctgag gaacaaggag agccagtcca 91440agtcctaaac tgaagaactt cgagtctgat gttcaagggc agggagcatc cagtaccaga 91500gaaagatgta ggctgggaag ctaggccagt ttagtctttt catgtttttc tgccaacttt 91560tcatattcta gctgcactgg cagctgatta gatggtgccc acccaggtta agcgtgggtc 91620tgcctttccc agcccactga ctcaaatgtt aatctccttt ggcaacacct tcacagacac 91680acctaggatc aatactttgc atccttcaat ccaatcaagt tgacactcag tattaaccat 91740cacagaagca ttatctacta ataccaccat ataaaagatg cggatgttag catagttatc 91800acactgtttc ctcacacaat ccactaccca tggcttggtt tctatggaca tattattgga 91860tctgagccag cttattataa tgttttaaat aatattcaag aattgttaca attgcatttt 91920gaattgttat tatttaaata taggtcaatt tctaaataga ttcttgtcca tagtcagatt 91980ttacatcatg atttcctcac tcatgacatt taaaagttta tttgttaaat ttagccaata 92040tatacaagtc tgcatttggg gatgggtaat tgaatgccat ttttaatttt tgggtaatgg 92100agactttttt tatgttgcct ttatatttga tccataacta ggctatgtag tacatttctg 92160ggtcaaatct ctattgcttt aaaactttgt ggttttgctt cattatctcc tagtatctac 92220agttactgaa gtgatacgta caaccagctt tggacatgat gaatgtttta ggctaaagac 92280ttgggtgttc tttggtcttt ggaagttttc tttaatactc tctttaaatt tgtctgattc 92340cacttcttgg ttcccttttc aggaacacca agtattcaca cagtggatag tcatctgtcc 92400tctacttcta aaattttgtt atttaacaaa atgttataaa agtagaatca catttatgta 92460acctttttgg attgatgttt tttgctcagc ataatttcct ggcaatccat ccaagttgtt 92520gcatgcatca atcatccatt cctttacatt gctaagtagt attccatgct atgtatttac 92580aacagtttgt ttacccattc acctgttgaa aaacatcttg gctgaatcta gttcttagct 92640attaatatta taaacaaaga tgctataaac atttgtttga cagttttttg tgtgaagttt 92700ttatttttct gggataaatg cctgggagtg caattgctgg gtcatatgat agttgtgtgt 92760ttttctttga gaaactccca aaatttccca gagtggatga agtattttat gtttctatca 92820gtaatgtatc agtgatccgg tttctacaca tccttaccag catttggtgg tgtcatgatt 92880tttaatttta gccattctga tagatgagta gtgatagctt attgttgttt caatttgcat 92940ttccctaaaa cctaataatt ttgaacatct tttcatgtga ttatttgctg tccatgaaat 93000atctgtggaa acagtgagta gaaggatgct ttccacagtc tgagaaggac agctgggaga 93060ggtggatgaa gagaggttgg tatatgggta caaaaataca attaagacct ctggtccatg 93120ctgggcacag tggctcatgc ctgcaatccc agcactttgg gaggccaaag cgggtggatc 93180acctgaggtc aggagcttaa gaccagcttg gccaacatgg tgaaaccctg tctctactga 93240acatacaaaa attagccggg catggtggtg cgtgcctgta atcccagcta ctcaggaggc 93300tgaggtggga gaatcactta aacccgggag gcgaagtttg cagtaagccg aggtcacacc 93360actgcactcc agcctgggca acagagcact ccagccgggg caacagagca agactctgtc 93420tcaaaaaaat aaaataaaat aaagtaaaac aaaataaaga gttccaatcc agcagagagt 93480gtggtgagat ggtgcgggac cagctccctg ggacttctga gcctttgccc tggccagtgg 93540gggagctgag ccaggatagg ctgtagcctc tgccccgtga caccaggacc cttgtggtgg 93600ctgccaccag gacccttggg gtgggtgcct gggccttgaa gatgcagcaa gcctcccttc 93660cccctacacc tcagtgactg agggtggtgg tggcctttcc caggccgcac cctgccgggt 93720atagacgaag tccccactga gcctggatgc agtgcctttg ggtggtggtg ggagtggcag 93780gggcagcttg gtgactatcc gggtgtgagg attgggaagg tgctcttggg atgcaggaca 93840cgggctcagc cacctcttta ggccatggcg ctgccagatc cgggtcctgg tgaccactct 93900gtcccagctg ggtgagactt acctagtcct gaggacaaca gacaatggcc ttaataggcc 93960tggaaggtga gtggaggcac agaggaggac agtgtggaac agttagcagg cattgggctg 94020ttggtcttcc ttctaccaga cctggaagga cctcacattt ggcctatggg aaatgcccgc 94080cacactttgg gaagatttac tatctgcttc tagaaggctg tgtgtatatt atgaaaaagc 94140tattctcaac tcacccccaa ccttttaata gataacatct gtggggaggc tgacaagatg 94200gctgaataga aacagctcag gtgtgcagct cacagtgaga tcaatgcaga aggtgggtga 94260tttctgcatt tccaactgag gtacccggct catctcattt ggactggtta gacagtgggt 94320gcagcccatg gagggtgaac tgaggcaggg tggggtgttg cctcacctgg gaagtgcaag 94380gggtcagaaa actcccctgt tagccaaggg aagctgtaag gagctgtgcc atgaggagtg 94440gtgcattcca gcccagatac tatgcttttc ccatggtctt tgcaacccac agaccaggag 94500attccctcca gtgcttatgc caccagggtc ttgggtttca agcacaaaac tgggtggcca 94560tttgggtaga caccaaggta gctgcaggag tcttttttca taccccaggg atgcctggaa 94620tgtcagcgag acagaaccat tcactcccct ggaaaggggg ccgaagccag ggaaccaggt 94680ggtctagctc agtggatccc acccccatgg agcccagcaa gctaagatcc actggcttga 94740aattcttgct gccagcacag tagtctgaag ttgacctggg atgctggagc ttggtatggg 94800gaggggcgtc tgccattact gaggcttgag taggcagtgt tcctctcaca gtgtaaacaa 94860aactgcaagg aagttccaac tgggtggaac acaccacagc gctgccaggc tgctgtagca 94920agactgcctc tctagattcc tcctgtctgg gcagggcatc tctgaaaaaa aggcagaagc 94980cccagtcagg gacttataga taaaactccc atctctctgg gacagagcac ctgggggaag 95040gggcagctgt gggtgcagct tcagcagatt ttaacatccc tgcctgccag ctctgaagag 95100agcagtggat ctcccagcac agtgtttgag cagactgcca cctcaagtgg gtccctgacc 95160ccctggcctc ctgaccggga gataccttcc agaagaagcc aacagacacc tcacacaaga 95220gagctccagc tggcatctgg caggtgcccc tctgggacaa agctttcaga ggaaggaaca 95280ggcagcaatc tttgctgttc tgcagcctcc actggtgata cccaggcaaa caaggtctga 95340agtagatgtc cagcacactc cagcagacct gcagcagaga ggcctgactg ttagaagaaa 95400aactaacaaa cagaaaggaa tagcatcaac atcaacaaaa aggacatcca ctcagaaacc 95460ccatccaaag gtcaccaaca tcaaacacaa aaggtaggta aatccatgaa gatggggaga 95520aaccagcaca aaaaggctga aaattccaaa aaccagaatg cctcttcttc tccaaaaaat 95580cacaacttct caccatcaag ggaacaaaac tggatggaga atgaatttga tgaattggca 95640gaagtaggct tcaaaggtgg ataataacaa actcctctga gctaaaggag aatgttctaa 95700cccaacgcaa ggaagcaaag aaacttgaaa aaagattaga tgaattgcta actagaataa 95760cgagtttaga gaagaacata aatgacctga tggagctgaa aaacacagca caagaactta 95820gtgaagcata cacacatatc aatagctgaa tcgatcaagt ggaagaaagg atatcagaga 95880ttgaagatca acttgcagaa accctataag ccagaagaga ctaggggcca atattcaaca 95940ttcttaaaga aaagaatttt caatgctgaa tttcatatcc agccaaacta agcttcataa 96000gtgaaggaga aataaaattc tttacagaca agcaaatgtg agacattttg tcgtcaccac 96060gcctgcatta caagagctcc tgaagggagc aataagcatg gaaaggaaaa aatggtgcca 96120gccactgcaa aaacatgcca aatggtaaag accatcaacg ctatgaagaa actgcatcaa 96180gtaatgggca aaataaccag ctagcatcat aatgatagga tcaaatttac acataacata 96240ttaaccttaa atgtaaatgg gctaaatgcc ccaattaaaa gacacagact ggcaacatgg 96300ataaagagtc aagacccatc agtgtgcagt attcaggaga cccatctcac atgcaaaaac 96360acacataggc ccaaaataaa gggatggagg aatatttacc aagcaaatgg aaagcaaaaa 96420acaaacaaac aaacaaacaa acaaacaaac aggggttgca atcatagtct ctgataaaac 96480agactttaaa ccaacaaaga tcaaaagaga aaaagaaagc cattacatag tggctaagaa 96540gagctaacta tcctaaatat acatgcaccc aatacaggag cacccagatt cataaggcaa 96600gttcttaaac acctacaagg agacttagac tcccacacaa taatagtggg agattttaat 96660accccactgt caatatcaga caggtcaatg agacagaaaa ttaacaagga tatctaggaa 96720ttgaactcag ctccagacta agcagaccta atagacatct atagaactct ccaccccaaa 96780tcaacagaat atacattctt ctcagcacca catcgcactt attctaaaat tgaccacata 96840attggaagta aaacactcct cagcaaatgc agaagaatgg aaatcataac aaacagtctc 96900tcacaccaca gcgcaattaa attagaattc agaattaaga aactcactca aaacctcaca 96960cttacatgga aactgaacaa cctgctcctg aatgactgct ggataaataa tgaaatgaag 97020aaggataaag atgttgtttg aaaacaataa gaataatgta ccagaaactc tgggacatat 97080ttaaagcagt gtgtagagga aaatttatag cagtaattgc ctacaaaaga acacaggaaa 97140gatctaaaat cgacacccta acatcacaat taaaagaact agagaagcaa gagcaaacaa 97200attcaaaagc tagcagaaaa caagagataa ctaagatcag ggcagaactg aaagagatag 97260agacatgaaa aacccttaaa aatcaattaa tcgctctccc tctccctctc cctctccctc 97320tccgtctccc tctccgtctc cctctcccca cggtctccct ctccctctct ttccacggtc 97380tccctctgat gccgagccga agctggactg tactgctgcc atctcggctc actgcaacct 97440ccctgcctga ttctcctgcc tcagcctgcc gagtgcctgc gattgcaggt gcgcgccgcc 97500acgcctgact ggttttctct gcccggccag ccgccccgtc cgggagggag gtaggaggtc 97560agccccccgc ccggccagcc gccccatccg ggaggtgagg ggtgcctctg cccggccgcc 97620cctactggga agtgaggagc ccctctgccc agccaccacc ccgtctggga ggtgtaccca 97680acagctcatt gagaaggggc catgatgaca atggcggttt tgtggaatag aaagggggga 97740aaggtgggga aaagattgag aaatcggatg gttgccgtgt ctgtgtagaa agaagtagac 97800atgggagact tttcattttg ttctgtacta agaaaaattc ttctgccgtg ggatcctgtg 97860gatctgtgac cttaccccca accctgtgct ctctgaaaca tgtgctgtgt ccactcatgg 97920ttaaatggat taagggtggt gcaagatgtg ctttgttaaa cagatgcttg aaggcagcat 97980gctccttaag agtcatcacc actccctaat ctcaagtacc cagggacaca aacactgcgg 98040aaggcagcag ggtcctctgc ttgggaaaac cagagacctt tgttcacttg tttatctgct 98100gaccctccct ccattattgt cctatgaccc tgccaaatcc ccctctgcga gaaacaccca 98160agaatgatca ataaaaaaat aaataaataa aaataaaaat aaaataaaat aaaataaaaa 98220aagaaataaa cacaaataaa atgttaaaat tcccattcta cataaaatca tctatagatg 98280tttttaaata tacaacatga ttatcaagga cttaatctgt accacattct tgtagatgta 98340aagttaaaat tcttgtaacc atcactctca accctaccct atcttccagc ataaatgaga 98400ctttcacatg ttttctcttg cccagatgga gacactaact gtaggctttg gaaatgtctt 98460aaaaaaaaaa aaaaaaaaaa gagagccagg catcttcaaa cagagaaaag ctgtttgaac 98520acatttatct aagtctatca aaagctatga ttgttagcaa gaggtaacct tgtaatgcca 98580aggcagaaaa ctgccaccaa accacaaaaa gcttgttcaa ctgctgaacc taaaattaaa 98640caatttatct ctcattaaaa aaaaaaaatc aattaatcca ggagctggtt tttttttaaa 98700gatcaacaaa atagatagac cactagccag actaataaag aggaaaagag agaagagtca 98760aatagacata ataaaaaatg ataaaggaga tatcaccact gatcccacag aaatacaacc 98820taccatcaga gaatattata aacacctcta tgcaaataaa ccagaaaatc tagaagaaat 98880ggataaattc ctggacacat acaccctccc aagactaaac caggaagaag tcaaatccct 98940gaataagcca ataacaaact ctgaaattga ggtggtaatt aatagcctac caaccaaaaa 99000aagtccagga ccagacggat tcacagctga attctaccag aggtacaaaa ggagctggta 99060ccattccttc tgaaactatt ccaaacaata gaaaaagaga gaatctccct aactcatttt 99120acgaggccag catcatcctg ataccaaagc ctggcagaga cacaacaaaa aaagaaaatt 99180tcaggccgaa atccctaatg aatattgatg caaaaatcct cagtaaaatt ctggcaaacc 99240gaatccagca gcacatcaaa aagcttatcc accatgatca agttggcttc atccctggga 99300tgcaaggctg gttcaacata tgcaaattaa tgaacgtaat gcatcacata aacagaacca 99360atgacaaaaa ccatgtgatt atctcaatag atgcagaaaa gaccttcgac aaaattcaac 99420agcccttcac gctaaaaact ctcaataaac tagatattca tggaacatgt ctcaaaacaa 99480tcagagctat ttatgagaaa cccacagcag ccaatatcat actgaatggg caaaaactgg 99540aagcattccc tttgaaaact ggcataagac aaggatgccc tctctcacca ctcctattca 99600acatagtatt gggagttctg gccagggcaa tcaggcaaca gaaagaaatg aaggatattc 99660aattaggaaa agaggaagtc aaattgtctc tgtttgcaga tgacatgact gtatatttag 99720aaaaccccat catctcagcc caaaatctcc ttaagctgat aagcaacttc agcaaagtct 99780tgggatacaa aatcaatgtg caaaaatcac aagcattcct atacaccaat aacagacaaa 99840ccgagagcca aatcacgagt gaactcccat tcacagttgc tgcaaagaga ataaaatact 99900taggaatcca acttacaagg gatgtgaagg acctcttcaa ggagaactac aaatcactgc 99960tcaatgacat cagagaggac acaaacaaat ggaaaaacat tccatgctca tggataggaa 100020gaatcaatat cgtgaaaatg gccataatat ccaaagtaat ttatagattg aatgctatcc 100080ccatcaagct accactgact ttcttcacag aattggaaaa aactacttta aatttcatat 100140agaaccaaaa aagagcctgc atagccaagc caatcttaag caaaaataac aaagctggag 100200gcatcacact acctgacttt aaactatact acatggctgc agtaaccaaa acagcatggt 100260actagtacca aaacagatat atagaccaat ggaacagaac agaggcctca gaaataacac 100320cacacatcta caactatctg atctttgaca aacctgacaa aaacaagcaa tggggaaagg 100380attctctact taataaatgg cattgggaaa actggttagc catatgcaga aagctgaaac 100440tagatccctt ccttccactg tatacaaaaa ttaactcaag atgggctaaa gacttaaatg 100500taagacctaa aaccataaaa accctaaaag aaaacatagg taataccatt caggacatag 100560gcatgggcaa agacttcatg actaaaacac aaaaagcaat ggcaacaaaa gccaaaattg 100620acaaatggga tctaattaaa ctaaggagct tctgcacagc gaaagtaact accatcagag 100680tgaacaggca accaacagaa tgggagaaca tttttgcaat ctatccatct aacaaagggc 100740taatatccag aatctacaaa gaacttaaac aaatttacaa gaaaaaacaa acaattccat 100800caaaaagtgg gtgaaggata agaaaagact cttctcaaaa gaagacattt atgcagtcaa 100860caagcatagg aaaaaaagct catcatcact ggtcattaga gaagtgcaaa tcaaaaacac 100920aatgagatac catttcatgc cagttagaat ggccatcatt aaaaagtcaa gaaacaacag 100980atactagaga gcatgtggag aaataggacc acttttacac tgttggtggg agtggaaatt 101040agttcaacca ttgtggaaga cagtgtggta ttcctcatct tcaccacggg gtggaggtac 101100gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 101160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagaaacata gaccctgccc 101220aacgcatatt cctgaaaggg atggcaaggt ttttttaaag gttttatggg tttaggtgta 101280ctctaaagga ttttaaccct tctaaaatta tttttgtata aaggtgtaag aaagggatcc 101340aacttcactc ttttacaaat gggaagccag tttttcccgg cccctttttt taaaaaggat 101400tccctttccc attggttgtt tttttcaagg ttgtcaaaga tcagaaggtt gtagatgtgg 101460gttggtaatt tttaggggtt tgttttggtc ccattggtcc ataggtcggt tgtggtacca 101520gggccatgtt gttttggtta ctgtaagcct cgtagtatgg tttgaagtca ggtagcatga 101580tgcctccagc tctgttcttt tggcttagga ttgtcttggc aatgcgggct cttttttggg 101640ttccatatga actttaaagt agtttttccc aattctgtgc agaaagtcat tggtagtttg 101700atggggatgg cattgatcta taaattacct tgggagtatg gccattttca tgatattgat 101760tcttcctatc catgagcatg gaatgttctt ccatttgttt gtgtcctctt ttattttgtt 101820gagcagtggt ttgtagttct ccttgaagag gtccttcaca tcccttgtaa gttggattcc 101880taggtatttt attctctttg aagcaattgt gaatgggagt tcactcatga tttggctgtc 101940tgtctgttat tggtatataa gaatgcttgt gatttttgca cattgatttt gtatcctgag 102000actttgctga agttccttat cagcttaagg agattttggg ctgagacgat ggggttttct 102060agatataaaa tcatgtcatc tacaaacagg gacaatttga cttcctcttt tcctaattga 102120atacccttta tttccttctc ctgcctgatt gccctggcca gaacttccaa cactatgttg 102180aataggagtg gtgagagagg gcatccctgt cttgtgccag ttttcaaagg gaatgcttcc 102240agtttttgct ccttcagtat gatattggct gtgggtctgt cataaatagc tcttattatt 102300ttgagataca tcccatcaat acctaattta ttgagagttt tcagcatgaa tggttgttga 102360attttgtcaa aggccttttc tgcatctatt gagataatca tgtgtttttt gtcattggtt 102420ctgtttatat gctagattac atttattgat ttgcgtatgt tgaaccagcc ttccatccca 102480gggatgaagc ccacttgatc atggtggata agctttctga tgtactgctg gattcggttt 102540gccagtattt tattgaggat tttcgcattg atgttcatca gggatattgg tctaaaattc 102600tctttgtttg ttgtgtgtct gccaggcttt ggtatcggga tgatgctggc ctcatataat 102660gagttaggga ggattccctc tttttctatt gattggaata gtttcgccaa actgacatga 102720tttttaaaat tccctatagg gtcaatgtgc agatttaaag atccctacac tcagtagtcc 102780tgggataggg tcgggggatc catatgttta acaagcagcc tagagtctcc cgatagcctg 102840ggcaagcttt aaaaagccct gcggacattc ggatgaaaca aaggtgcaca gaacctatat 102900ctctgccatt tattagctgg gggccttagg caaggaaggt agtcaacctc cgtattcctc 102960agttcctcaa ctgtaaaatg gggatgatga ctgtggctcc ctcgtggggt tgtgacggag 103020gaacagggga catatgtcct gggatcagca ctggcaattt ggccattctt accatagcac 103080ctgccatctt ggtgacagcc tggacacaaa gccttcacac ctttgaagtg gccatagaga 103140gcatgagata tggatgggga ggaggcattc caactaaaga cccctgcttt gggccaaaaa 103200cacatgtatg tctggtgtct ggagtcctct ggttatgcct tttttaagat accggttgtc 103260ctgcaattgt gcgttgagaa tccactctat ggccaacata tcatcaaagc gtttggctca 103320gctaggctgt tcccccaacc caaggacccc atgaggaaag gagtgagggt tccaccccca 103380gcaccagcca ctggaggggc tgcccagagt ccctcagttc tcgtaaactt acgctttgga 103440gtaattcaga acaaagtcca ctcctttttc actatcaaac tggatatcac ggggtaaatc 103500cttgtggcat ttggcatcga tgctcaaggg gaagccaggg ttccactcca tccatctgag 103560catgagaaag gagaagaaat tgagttaaat gccagccttt ggtctgcccc ctcagctgag 103620ggccgcccac actgtcagac gatgttacac agggagattg atgtactcaa aataaagtca 103680acacatcata ggctgaccct gtgtccagta aggttctggg acctcttcca aagtccagtc 103740gcaccaactt tgctgtgccc tgagcatcag agtgggaacc ctgagactgg gcttatagtg 103800gtacaggtgt gcaccactgc ataaaaagtt ctgtaggtag tctagggaga cctcacttac 103860tagaggtgac attgaacagt agaggcctgc cttgccagtc actggaggta gaaggtatta 103920aggctgccca gccacgccca acctggacat ccaccattct gccaacttga tcagtgtgtg 103980ccctggactg cgtgcatgtc ctgcatgtgg catttgggga aatgggacag ttcttctgcc 104040ttcacaatag gacgacacgg cagcaggtgg cctgggagag ggacccaggc acagatggct 104100aaaaggcttc cctctctcac ctgctggcca atagcagctt tagagattat tacaatcaaa 104160acagcttccc caaagacaag ccacggactg catccagtaa acatgctcag atcctgacat 104220ccttgaaacg tatcccgtgg cattgggcat ttgtgttttt gaaaaatgtt tattttgctt 104280ttttgtttac ttattgagta aacttaaatt gtgaaaatgt cagaggtgtt tgaaccacag 104340caactccatc ttgaataggg gctgggtaaa ataaggctga gacctactgg gcagcattcc 104400caggaggtta ggcattctaa gtcacaggat gagacaggac attgatgcaa gatacagggc 104460agaaagacct tgctgataaa atgggttgtg gtaaagaagc cggccaaaac ccaacaaaac 104520taagatggtg atgaaagtga cctctgttca tcctcaccac tcattatatg ctaattataa 104580tgcattagca tgctaaaaga aactcccacc agtgccatga cagtttacaa atgccatagt 104640aatgtcagga agttacccta tatggtctaa aaaagggagg aaccttcagt tcctggaatt 104700gcccacccct ctcctggaaa actcatgaat aatccactca ttgtttagca catgatcacg 104760aagtgactgt aagtatgctc agtcgagtag cccatgctac tgccttgcct atggagtagc 104820ctttctttat tgctttactt tcctaataaa cttgctttca ctttatggat tcaccctgaa 104880ttctttcctg tggaggtcca agaactctct cttggggtct ggatcaggac ccctttctgg 104940taacaaaaac aaaatgaaat cctgttagtt tctttgggga gggagaatga ttttgtgttt 105000gtttaaaaac cagattttac attttagggt tttagctttt tcatttatat tcctaattct 105060ccttcctttt aaaattttct tttgagagga aagtaaaata tcgagatttt gggtcttaaa 105120aaagtcctga gttattttaa tatgatcttg tgctttgact ttccccaaat cccaactgtc 105180acagtgtcct cttggcccag tagcctctcc ttaggatcac aagaagtgaa cagggaccgg 105240gtatgatcct tctgacagca aaggcatttt gtcaagtgtt ttctgttatg tggcacctta 105300catttctcac tgttggttta tttagaatat tctacgtcct cagattttaa gaaaaccttc 105360catttttgct gtttgtatta atgccaatga tagtatatta acaaggataa aaatgtggac 105420aagtctacac attttttgtt tgtttgtttt ggaaaatgac tcaaggcatc tccaatatat 105480atctaaaatt gagccattca gtagcctcac tggtagtaat gatcaattaa ttagctatca 105540ttagtcatat caatttaaat attactgatg attttctctt tgcctggaca gttcaagggc 105600tctagttaat tttaaaagct cagtggagct agatgttttt cagtatgtaa acaatgagcc 105660atgcaagggc atctgtgcca gcaaatggca ttaatgtttt cttaagtgaa tagtccactt 105720ggacggatgt ggaatacttc ttcagcacca aaaagacctt cttgccccat gccctgccct 105780ggtctatgga gggtggcagc accaatgggc agaaatgccc cacttgtgtg gttagaagat 105840gatggcctca cagcacatgg agacaggaca gcttgcttgc tagttcacta ctgcccccct 105900gaaaccaaat gtttatgtcc cttccactgc aggtcactgc tgcttaagag accctgacag 105960gctgacctgc acaggagccg aaggcctgca tggccacaga cattaactgg tggagatgga 106020atcccactcc attgaggaag accaagtgga acacccaggc agaaggaaca aagaagtaga 106080aaggcattta atttattgga aaaaaaatga gagagagaga gaaagagaga gagagaaagc 106140aagagagaca gagagacaga gagagagaga gagagaaaga gagagagaga aagcaagaga 106200gacagagaga cagagagaga gagagagaga gagagacaga cagacagaga gatcctaagg 106260ggatgcagtt agtataattg gagtgtaaaa agactttctg taaccacaaa catgatttct 106320gaatgaagac ttcaatagag aaacagacaa agagaataat cactgtgaga tgtttggtct 106380cttgtattaa aagaaaagct ttagacaaat taaatttagc agtttaattg agcaaagaac 106440aattcaagaa tagggcagcc cctaaaccag agcaggctcc gagtgacttt gatgctgtct 106500catagttgga taggatttat gggcagaaaa aggaacgtga tgtacagaaa atggaagtga 106560ggtacagaaa cagccagatt ggtgatggct cagcatttgc cttacttgag cccagtttga 106620acagctggcc actgtgagtg gctgaagtct ggccgctgtg attggctgag acttggttac 106680ttgctatgag agtaggtgac agtctgttta cacatccagt taggttacag ttcactatgt 106740acagagaaac ttttaggctg aatttaaaat atgtaaggag aaaagtttaa gctaaactca 106800ttaacaccta gaagagagaa ggtgttcatt cgacaactaa ttatggagca cctactatgt 106860gccaggaagt gctttgtgca aaatagaaga acatgtaacc caaatacagg gtgaaactac 106920aaagaaatgg aagccataaa tgaaactatg agagacataa accaaaaata aaattctaag 106980cccctgaact gactgatgga ccctcccctt ggccaagggc attccaaagt taacctgaaa 107040aattagttca ggccatgata ggaatgggtg gctaaacgtg cctcattgta ctctcctcct 107100cttggaattc aggcacaact gatcagcatt cacatcaaca cagagatgtt aagcccatta 107160gaacagactc tttaagattg ataagaaaca tttacaattg attctacctg aagcctgcta 107220cctggaggct tcatctgcat gataaaaaac tttgtttcca caacccctta tcttaaccta 107280gacattccaa gtttttagat aataattctt ttaagcagtt gccaatcaga aactctttga 107340atctgcctat gacctggaag ctcccctccc ccaagttgtc ccacctttcc aaaaccaaac 107400cactgtccat cttacatgta ttgactgatg tcttatgtct ccctaaaatg cacaaaagca 107460agctgtaccc caaccacctt gggtgcatgt tctcaggatc tcctggggct gcatcatggg 107520ccactggtca ctcatatttg gctcagaata agtcttttca aatatttcac agagtgtgac 107580tcttttcatt aacagacatg aaagccagat acagatgtaa tatgcaagga gtaaaagctc 107640caaaaggaga gagagtaata tatggagaag tgaaaaaaat caaagaaata aaagaagaac 107700tcctttaagc tgaaaaatac ttcaatatct taagtctgtg aaaggaaatt aaattttagg 107760accccaaact catttagcca aagggaaatg tcaagctggg aactgtgtca tgcaaacctg 107820cctccccctt ttggttccta aataagatgg ctatgatgaa aagctacatg tctccaccat 107880attttgctca caaggaaatt cctagtgaac cgttaaaatt tcaccatggc aatgcaaatt 107940gatagcttat ctttacaagt gcagtcaccc accagataca aatgcatatc tgattgttcc 108000cctgccttac tttatctatg ttctcttatg taaaatgcag actccctgca tttttcctct 108060gcctcatttg tttatgtcat cttatgttaa aaaaaatgca gattcactga gccagagaaa 108120ggcatgaata actatttttc cctgccctgc tcttacatga aaattgtgta cttctcaata 108180tcccgccctt ttccctttaa atttggagcc ctcaaaatca ccttcagaga aaggcatagt 108240gctgtctccc gggcatgcgt ccttaacttt cgcaaataaa tttcctaaaa tgattgagac 108300ttgtctcgtc atttttctca atgacaagtc ctaggtgggg gggaaaaaaa aaaaggaaaa 108360agatacacct aggtatatac tgaatacatt ttaaaatgtc caagagacag gagaaacaga 108420gacagagaga aggaaagaga tgcagagagt gaacgagaga ggaagaaagt gggggaggga 108480gggagataga aactctagga aaagaaacaa actatcatta gatttctcct ctgtcattct 108540gaaaatggga agactgaagt gtatacacac tttttttaga tcgtcatcta agcctctcat 108600attgtcatca atgagggcag gggaatttgt ttgaggggat gggtaggggg acaggacgac 108660aagcacagac tcagatctta cactacacat ggaccttctg aggaaattaa ttgaacaagt 108720tcagctgaac aaaaattaaa ttagaatgaa tcaataaact cctaaataca gcaaagaata 108780tgcattataa aactacagca aatataatag taaacaaaga aacaaaaata tgagagggat 108840gttcatcact actaccacta cttgttttgg gaattccaga catggaataa gaaaagaata 108900tcagtggtgt agataatgga aagtaagaga caaaattatt aacatttaga taatatgagc 108960acattaacta accagactca ctaacaacaa acaaacaaaa acaagtgatt agaaccaatg 109020aaagaattca gtaagggacc aacacacagt aaatgtaaga aacaagtaat ttacatttat 109080cccagcaata tttttgcaaa cagaaaaata tcctactcag aacagcaaat gctaaatgta 109140gagtagctag aaaggtgaag gacctgcatg aagaaaacta gaaattctat ggaagtacgt 109200tgacacagga tctagagagg tggaaagaca cgctgtgttc ctggatggaa agactgaatc 109260tcagtaaaat gccaacacat aacacagtta tgtgaagttc aaagtaattc tcattatgaa 109320aaacttaaga aagaacagtg agatatgtaa cagacattaa catttattag aaagttatga 109380taaccaaaag ctggtgctgg ggtctcgagt agaaaaatag gctaacacat aaagtgcgga 109440aacagaccca gagactatgt atacataata caaaactcgc caacacttat ccagtgcttt 109500ctatgtgcct actactattc tctgtgtttt catggtttaa cctattatct caaaccccta 109560aaaggaaggc actgttattt ccctcatttt acagatgagg gaagtgaggc acagacagat 109620taaacaactt tcacaggact ggagaaagtg ccagtaagtg tgagttgagc ccagacactc 109680gaatccagag ctcatgactg ccatcctatg ccagcttgta ccgtgggtat catcatccct 109740gtcatccaag tcaggaggaa agacatcatc cctgtcatcc aagtcaggaa cgaatgctac 109800cgggagagtt ccacccaggt atgtatgaaa acacgtggca ggagtggggc atggtggcgc 109860acgcctgtag ccccagctac ttgggaggct gacgtgggaa gatggattga gcctgggtta 109920gtttgaggca gcagtgaacc atgatcatgc cactgcactc cagcctagga aacagagcaa 109980gactctgtct caaaaaaaaa aaaaaaggta gatgaatgaa agctctaaac tcaaagggga 110040aattataaaa gtattagaca aaattacggg ggaagtattt gtgtaacact gggatggaaa 110100gggttttctt aagcaaagga ggaaacccaa aagttataga tgtaaacaat acatagttaa 110160taaaataaag aactaaaatc tcctgtggca aaagcaacag aagacaaata ataaatgcta 110220tacatatgta acaaacggat actatcctga tatagaaaga gctcctttag attaataatt 110280gaaagaacaa tagggaaaaa aagcttataa ataggcaatg cagtgatgct tattagtggt 110340caggggagtg cagactaaaa caaccacaag gtcttaggga tttttagact gtcaaacaag 110400gtcatcgggt actgacaaat gtatggatgg gcaaaagtat cttttcatac acagctggta 110460ggaccgcaaa ttacgataca attttgtgaa aggatttttg tcggtatttc ttaagatgaa 110520aaaatgctga tttgttccag caatctgagt tctggaaatg gatctgccat taattaaagc 110580accagtacat caagatgttt gtacaaggtt ggttctggca gcattgtttg tactgcaata 110640cccaaaacaa acaaacacat ctacaaatgg gaaatgaact gaacgtcagt gaatagggga 110700atggctaagt aaattatggt gcattcccag aaggcaagaa atagtagcag tttaagaagg 110760ggaaaatata tgagatacta tttcacacgc aagaatgaaa acatctgaag atgcgaagtg 110820ttgatgatgc agggaaacag tgaatctcag aaacagttac tggggtgtct actggcagaa 110880tgcctttgga aaacaatcag caatatctag aaagcttaaa aacacgtggt ctgcaaccct 110940gcaactctac ttcttggaat attccctaca gtaactcttc catgagagca cagagagatg 111000tgtgagaaag ctcactgtcg cttggtttgc aacagctaga aattaaagac agcccgcctg 111060cccatcagca ggagagcgga cgagcgcatg ggttattcag atactgatat gccatgaaga 111120agttaactgc atgaaccaca tctatctgtg tcaaccagat cctcttcaat acttagtgat 111180gagtgaaaag tgcaggtcac ataatgatta gattaaatta tatcgattaa caaacaccta 111240cactttgcca tttactgctg tgtgtgagtg tgcatgtaac acagacagat gtggtcacag 111300tgggagagca caagctgtgg gctatgacct gggtctgctt ctccttctcc ctgcgtggct 111360gagtgagtaa ggagggcctg caagaagcgc cagctttctg acatagtgtg gactattctt 111420agtcattgct ttacttccaa actctgatct ggaagcctga gaaatcttct gagaaagagg 111480aactccaaga ttgtgcagac agtgcaaagt tcagtgtttc ctgaacggaa gcaaaggagg 111540gtttctgaaa aggacagatg agtggaagga ggaagaaaaa aatgattaaa tctaagaaag 111600ggggaaacga tctcaccaga agccccctgc tgcaagatta tgataatgtt aagtagctcc 111660cagataattg ggctagtttg tgttatctta gatgagtaga tagcaaagtc taattagagc 111720cccactaatt catactaaag tgttagtggc ttataacaaa ttaaatgaat tttatgggca 111780ttttatctta acccagattc acaggccaga agtgctctga gaactggctg caggggcttt 111840gccataggtg tctgaaacag ggagggaagc caaggaagaa gcatagaaac aacagccctt 111900tgggatggga ggcagagctc aagcacaacg tgtgcactgc accaggtatg gaagtctgga 111960aacacatcat atggtgcctg agctgagagg cttgtgcaca aatactctaa aacaaatcac 112020acatttataa acccttaaca tgacccacag cacagcaggt tactctccca cacttgatgc 112080taatggcatg taagttttat ttttgatttt tcaattggga aatagaatat ataaaatatg 112140tatggatatg tatatatgca cacacacact cacacatgca gaaaaatgta caaaacacct 112200gtacgaacac catccaggta aaaaatcaga acattgtccg taccctcacc cccacctttc 112260ccaatcaacc ctccttccat ccccggtaat gaccatcctc ggctttatga taatcatttt 112320cttgttattg tttaaggttc cgctatgaca cacctcccta aacaatttca ttccaaagtt 112380atgaattggc cagacacgct cctggcttct ttccttcaac actgcgtcag tgaggtttgt 112440ccatgttagc agcagtttgt tgattttcct tgcagtgtag aattttcctt gacaatttgt 112500ctgttctact gtttgttgga cattgagttg ttgccatttt ttggccatca tgagtaatgc 112560tgttgtgagt atccaggcgc acatgcacac aaatttggat cccactatag gatttctggg 112620tcatagatta taatgtagct tcagttttat gacatattgc taaattgttt tccacggagg 112680tcttaccaac ttgtcctcct gccggtggta tatgagtgtt cccagccctc tggatcttct 112740ttaatactca ggtgttgtca gttgttgaag tgttgcccat ctgggggcat atgtgtggta 112800gtagcagcag ttcactgtga tcttctgatt tccccgagtc ctaatgaagt tgaacacctt 112860ttgtatactt gctgcttata ttttgtacag taagtactca aacttcttgc ccattttcta 112920gtgaatgaca ctccccttct taaagatttg taggtccttc acatactagg aataattctc 112980gtcaatcact ccatgacttg ccttcactct ctcctggtgt ctttcaatga acagagtgtc 113040cttatcttga gggaggcaaa cttaccaatc ttttcctttg tgtttaatat gttctgagtc 113100ttgtttgaaa aatcctttta gcttgagacc tggaagaata atcacctgta gtctaaatgt 113160cttacagttt tgcctttaac ttttaagtct aatcaacccc aagctgagtc tcctgaatgg 113220caagaggtgg agtctgattt cttctccttc tccttcagcc ctccgtgctg gctggcccct 113280gtcctcgaga tgcatgtcca gtggcccagt gccacccatc agccgagccc tccgtagcag 113340acccagtacc cacatacgtg tggctgtttg ttgtttcttt attctgtttc aaggaatgtt 113400tttgatgaaa agggggatga agctaagaca gttttgaggt ggggcaattg tgttcttggt 113460gtgggatttc tcaccccttg acagcagtgg gtggctgggc ataggggaag aaggagctac 113520agggttgagg gatgctgttg caacttagaa aaattaagaa aaatgaagat attgctcagt 113580tatggcaagt gtttgatgaa acagggaccc atacatggct cgtgaaaact ggttaaagat 113640tttttttttt tttttttttg agacggagtc tcgctctgtc gcccaggctg gagtgcagtg 113700gtgcaatctc ggctcactgc aagctccgcc tcccaggttc acgccattct cttgcctcag 113760cctcccgagc agctgggact acaggcgccc gccaccacgc ccggctaatt ttttgtattt 113820tttagtagag acggggtttc accgtgttag ccaggatggt ctcgatctcc tgacctggtg 113880atccacccac ctcagcctcc caaatggtta aagatttttg aggagaaatt tgtcaatgta 113940tactaataac ttttactatt tggtaatttc atatctgggc atctgtccta ggaaaatact 114000taataatgta tacaaatttc acatacaagg gttttcactt tgcttataag aatacaaaaa 114060ttagaaacct cctaaatgtc cagggaagct gggtgtttaa gtgaatgatg aggcatccat 114120ccaatggaat agtacataga aactagaata atgtttatga tgagattttg aaaatatggg 114180aaaatgattt ttgctacact gctaaagggc agcaaggagg atgcaaagtg gtatatatag 114240cacaatccca aattttaaaa agtgaataag acaaagtgaa aacaattaaa catgaatttt 114300tatgaaaaag ttcataaaat tcataattca tacagaaaat aggtattttt ctggctctat 114360attattttcc ttcttttact tttttgagtt aaaaaaacaa cccagatata ctacatattc 114420cttgtaagaa gttcaactat tgagggatat gtagaaaagt atcacaatat tggctcccaa 114480aggcccctcc acagcccagg taacttcact gtccatgtag aatgattcct tccactttct 114540tcccttggaa ggaggcacag actcaaatgt ttccaggggc caaagcagga aatgaaatga 114600aagaaaacct tgatatagac aacagacaag tgtggactgg ggaagcctga agaatatctc 114660tgtttactca taagtcaata atgatccatc aaacacttgt agaggctgga tacagcccat 114720cagccaccca tttccagagg agcactctgt aatgtgttat tatcttccaa aaagctttac 114780tgagatacat ttacatacca ttaaattcac cccttaaaaa tatgctatca aaagatttta 114840atatatttgc agagttgtat gtgtaaccat cattataacc caattctgaa ataatctcat 114900ccccagagga cacctcgaac ccattaacat tcactccttg tttcccctcc ccaactccta 114960gtgctaggca actactcacc tactaacctg actctgtggg tttgcctatt ctggacattt 115020catacagata gaatcacaca atatggggtc ttccgtaagt gagttttcca tttagcatat 115080ttttgaagtt cattcagtgt ttggcatcta tctgtagttc atttcttttc ttttcttttc 115140tttttttgaa atggagtctt gctctgtcac ccaggctgga gtgcagtggc gggatctagg 115200ctcactgcaa cctccgcctc ccgggttcaa gtgattctcc tgccttagcc tcccaagcag 115260ctgggattac aggcgtgcac caacatgccc ggctaatttt tgtatttttg tagacatggg 115320gtttcaccat gttggccagg ttggtctcaa actcctgacc tcaagtgatc tgcccgtctc 115380aacctcccaa agtgctagga ttacaggtgt gagcctccgc gcttggcatt cattcctttt 115440tgatgccaaa aaatatccca ttgtatggat aaactatatt ttgtttattc attcctcagt 115500taatggaatt taggttgttt ctactttttg actattgtga acaatggtgc tataaatatg 115560tgtgtacaag ttttgtatag acatgtggtt tcaatattct tgggtagagg cctaggagta 115620gaattgctgg gtcatattgt agctgggttt cagattttga ggaaccacta aactgttttc 115680tgaagtggct gcatcatctt acattccggt agcagcatca gggttccagt ttctccacat 115740ccttggcagc acttgttact atctgtcttt tttattctaa ccattttagt ggttagaatt 115800gtatcttgtg gttttgattt gaaatttcct gatgattaat aatgttgagc atattttcac 115860gtgcttattg gagattcaca aattttctct ggagaactat ttaattaaat cctttgctaa 115920tttttaaacg gggttttttg tacagaatat tttaaaattg agttgtcaga gttctttata 115980tattctggat acaagtccct tatcagacat atgatttaga tattttctcc attctgtgga 116040ttgtcttttc actttattga tgatgtcctt tgaagcatag atcttttaca ttttaatgac 116100atctaacttg tctactttct tttgctgcct gtgctcttgg tgccatatct aagagacttt 116160gccgaaccca agattatgaa gacttacttg tatgttttct tccaagagtg ttatgttgtt 116220agcccttact tttagatcta tcatccattt tgaattaatt tttaaattta tctagcagcg 116280ttattgaaat ataatgcaca tactgtaaaa tttatccatt taaagtgttc aattcaagga 116340tgtttagtat taatttttac acaatggaca accatcagtt ttagaacatt ttcatccccg 116400gcaaaaggaa cactgtatcc tttagctgtc actcccatga ccacttcctc attccctgcc 116460cctaggcaac cagtaatcta ctttatatct gtacagattt ttcttttcca gaaatttcat 116520ataaaaggaa tcatacaata tatgatcttt tgtgactggt ttctttcact tagcataatg 116580tttttgagat tcatccatgt tgtaacaagt gtcagtattt cattcttctt tcatggtcaa 116640attatataga tatactatat tttatccatt catccattaa tggacgtttg ggttgtgtac 116700actttttgat agttacaggt catgctgcta taatcatgca tgtacaagtt tttgtgtggg 116760catatgtttc catttctgtt gggaatatgc ttaagagtgg aaatgctgtc atattgtaac 116820tctctgttga acagtcagga gctagcagac tgtttcccaa gcagctgtgc cattttacat 116880tcccaccagc agtgtatgag ggttctgatt tctccacatc cttgtcaaca cttgttcttg 116940tcggattttt ttattctagc catcctagta tcaagtggta tctcattgtg gtgttgattt 117000gcatttccta gatgactaat gggtccttgg agaaatatct attcagatcc tcactcattt 117060ttttcatttt ttaattggac tatttgtctt tattgagttg taagagttct ttataaatta 117120tggatggaag gcccttatca gatatacaat ttgtaaatat ttcttccatt ctgtggttca 117180tcttttcact ttcttgatgg tgtcctttga aacataaagg tttgtaaatt tgatgacatc 117240actttatcac tttatctatt tttcttttgt tgctgatgct tttggtgtca tatcaagagt 117300cctttgtcaa atccaaagtc atgaaggttt actcctgtgt tttcttaaaa gagttttata 117360gttttagctc ttacatttag gtaggtattt gactcatttt gtgttaattt atgtatatgg 117420tgtgagctaa ggatccaact tcattctttt gcatgtggct atccagtttt gctaggacca 117480tttgttgaaa agactattct ttctctgtta aactgtcttg ctgcttctgt caaaaattaa 117540ttggccataa gtgtaagggc ttatttctgg actctcaagt ctatttcatt gatctatata 117600tctatcctta tgccgatatc acattgtctt gaatactata gctgtgtagt aattttgata 117660ttgagaagtg caagtcctcc atctttgttt tctttttatc aagattgttt tggctattct 117720gggtcccttc catttccata tgaatttgca gaattgacag cttgtcaatt tctgcaaaga 117780aatcagctag aattttgata agggttgtgt tgaacgtgta cattaatttg tggggtattg 117840ccattttaac aaaattaagt cctctgattc atgaacatga aatgtctttc aacctattta 117900gatcttcttt aataatgtta aacaatgttt tgtagtttca ttgtacaagt ctcagactac 117960tttgttaaat ttattcataa atgtttactg tttttgatgc tattgtaaat tgaacttttt 118020cttactttta tttcacatag ttggttgtta atgtatagaa aaacaattga ttatcttgta 118080tcttgcaatc ttggtggttt attagttcta ataatttctt ttggtgaatg ccttcagatt 118140ttctatatat aagatcatgt tatttgtaaa ttgtggtagt tttacttact gtactccatt 118200gtgggtgcct ttacttatct ttttattccc taactgagct ggctagaccc tccagtacaa 118260tgttgaatag aagtagcaaa agtgaacatc tttatcctgt ttctggattc agcctttcat 118320cattaagtat aatgttaact gtgaattttc tttttttgtg tatgtggggg gacggagtct 118380tgcttgtcac catgctggag tgcagtggca tgatctcagc tcactgcaac ctccgccttc 118440tgggttcaag tgattctcct gcctcagcct cctgagtagc tgggactaca ggcgcatgcc 118500accacacaca gctacttttt gtattttagt atagacgggg tttcaccatg ttggccagga 118560tggtctcaat ctcttgacat cgtgatccac ccgccttggc ctcccaaagt gctgggatta 118620caggtgtgaa ccaccacgcc cagcctaatt gtgaattttc aaacctttat caggttgagg 118680acattttctt caattcctat tttttgagtg ttttatcatg aatgggtgtt ggattttgtc 118740aaatgccttt tttgtatgtc tattgaaatg attatgtact ttgttttgtt ttgttattta 118800ctctattgat atggtgaatt caattaattg atttttggat gttaaaccaa ctttgcattc 118860ctgggctaaa tcccacatgt tcatattgtt taatgccttt tatatacttc tgggttaggt 118920ttgctagtat ttcgttgaaa atgtttatat ctatattgct aagggatatt ggtttatagt 118980tttcttgtga tgcctttagt tttggtataa tatgggtatg agacaatgaa ttgggaagtg 119040ttccttctat ttcttaaaag agtttatgca agatcagtat taattcttta aatgtttgat 119100agaattagcc agtgaagcta tctgggtctg ggcttctatt tgtgggaaat tttagaatta 119160gtaattcaat ctctttggtt gtgattgttt tattcagatt aactatttct tcatgagtca 119220gttttggcag tttgcgtctt tctaggaatt tgcgcatttc atttaactag tttgttggca 119280tgcaattgtt catagtattc ttttttatta attttaattt ctgtattgat tttccctcat 119340tttatgagtc tcatttttcc ttttttattc attttaattt ctgtattgat tttctcttat 119400tgctcatttt aatggtttga atcctctggt cagttggctg aagagttgtc aattttattg 119460ctgatcatgt atcactttta taattgaatt gttataagaa gacataagaa gtaagaggca 119520acccctacaa tggagaacat taaggtacac tcaagctttt ctgcccattc ttcctacatg 119580ccaatatggt tgatccaaag ttattttcat tactctcctc tccatctctt gtctggagtt 119640gtaggtaagg tgaagtttgg ggattgtgca cccacactgg gagctggtgc cttggtgact 119700ttccagcagt gacaggccat acactgtgga aagggtttat ttcataccat gctcttgaga 119760tagaagaaac catggccccg tggccactcg atctgatgtc ataactcacc gatattgttt 119820ttgccgtgtt tccagttctt tacgtcggtg ttgcttgaga atgtgaattt ggtcatctcg 119880ggccaacttt gctgtaaaga aagaacaaca tgagaggagc ctgaggaaga acaataacaa 119940tgcccaatgc caagtgttca ggcttccctc agatgccctg cccatgccga gtgctttatg 120000tgcatgacct catttgagcc tcagagcact tccccaatta atgatgaaga aactgaggct 120060tagggaagtt gtgtagcttt gctcttgaac tttacccaag gtcacacacc taattagtga 120120attttctttt ttttttttta attctacttt aagttctagg atacatgtgc acaacgtgca 120180ggtttgttac atatgtatac atgtgtcatg ttggtgtgct gcacccatta actcgtcatt 120240tacattagtt atatctccta atgctatccc tcccccttac ccccacccca tgacagacct 120300tggtgtgtga tgttcccctt cctgtgtcca agtgttctca ttgttcaatt cccacctatg 120360agtgagaaca tgtagtgttt gtttttttgt ccttgcgata gtttgctgag aagggtgatt 120420tccagcttca tccatgtccc tacaaaggac atgaattcat ccttttttat ggctgcatag 120480tattccatgg tgtatatgtg ccacattttc ttaattcagt ctatcattga tggacatttg 120540ggttggttcc aagtctttgc tattgtgaat agtgctgcaa taaacatacg tgtgcatgtg 120600tctttatagc agcatgattt ataatccttt gggtatatac ccagtaatgg gatggctggg 120660tcaaatggta tttctagttc tagatccctg aggaattgcc acagtgtctt ccacaaaggt 120720tgaactagtt tacagtccca ccaacagtat aaaagtgttc ccatttctcc acaccctctc 120780cagcacctgt tgtttcctga ctttttaatg attgccattg taactggtgt gagatggtat 120840atcattatgg ttttgatttg catttctctg atggccagtg atgatgagca ttttttcatg 120900tgtctgttgg ctgcataaac gtcttctttt gagaagtgtc tgttcatatc cttcgcccac 120960tttttgatgg ggttgtttgt ttttttcttg taaatttgtt tgagttgttt gtagattctg 121020gatattagcc ctttgtcaga tgagtagatt gcaaaatttt tttcccattc tgtaggttgc 121080ctgttcactc tgatggtagt ttcttttgct gtgcagaagc tctttagttt aattagatcc 121140catttgtcca ttttggcttt tgttgccatt gcttttggtg ttttagacat gaagtccttg 121200cccatgctta tgtcctgaat ggtattgcct aggttttcct ctagggtttt gatggtttta 121260ggtctaacat ttaagtcttt aatccacctt gaattaattt ttgtataagg tgtaaggaag 121320ggatccagct tcagctgtct acctatggct agccagtttt cccagcacca tttattaaat 121380agggaatcct ttccccattg cttgtttttg tcaggtttgt caaagatcag atggttgtag 121440atgtgtggtg tcatttctga gggctctgtt ctgttccatt ggtctatatc tctgttttgg 121500taccagtacc atgctgtttt ggttactgta gccttgtagt atagtttgaa gtcaggtagc 121560atgatgcctc cagctttgtt cttttggctt aggattgtct tggcaatgcg ggctcttttt 121620tggttccata tgaactttaa cgtagatttt tccaattctg agaagaaagt cattggtagt 121680ttgatgggga tgacattgaa tctataaatt accttgggag tatggccatt tttatgatat 121740tcattcttcc tatccatgag catgggatgt tcttccattt gtttgtgtct tcctttattt 121800cgttgagcag tgatttgtag ttctccttga agaggtcctt cacatccctt gtaagttgga 121860ttcttaggta ttttattctc tttgtagcaa ttgtgaatgg gagttcactc ctgatttggc 121920tctctgtttg tctgttattg gtgtataaga atgcttgtga ttcttgcaca ttgattttgt 121980atcctgagac tttgctgaag ttgcctatca gctttaagga gattttgggc tgagacaatg 122040gggttttcta aatatacaat catgtcatct ggaaacaggg acaatttgac ttcctctttt 122100cctaattaaa taccctttat ttctttctcc tgcctgattg ccctggccag aacttccaac 122160actatgttga ataggagtgg tgagagaggg catccctgtc ttgtgccagt tttcaaaggg 122220aatgcttcca gtttttgccc attcagtatg atattggttg tgggtttgtc ataaatagct 122280cttattattt tgagatacgt cccatcaata cctaatttat tgagagtttt tagcatgaat 122340ggttgttgaa ttttgtcaaa ggccttttct gcatctattg agataatcat gtggtttttg 122400tctttggttc tgtttatatg ctggattaca tttattgatt tgtgtatgtt gaaccagctt 122460tgcatcccag ggatgatgcc cacttgatcc tggtggataa gctttttgat gtgctgctgg 122520attcggtttg ccagtatttt attgaggatt tttgcatcga tgttcatcag ggatattggt 122580ctaaaattgt tttttttgtg tgtgtatctg ccaggctttg gtatcaggat gatgctggcc 122640tcataaaatg agttagggag gattccctct ttttctattg attggaatag tttcagaagg 122700aatggtacca gctcctcctt gtacctctgg tagaattcgg ctgtgaatcc atctggtcct 122760gaacttttct gggttggtag gctgttaatt attgcctcaa tttcagagcc tgttattggt 122820ctattcaggg attcaacttc ttcctggttt agtcttggga gggtgtatgt gtcgaggaat 122880ttatccattt tttctagatt ttctagttaa tttgcttaga gttgtttata gtattctctg 122940atggtagttt gtatttctgt ggaatccgtg gtgatgtccc ctttatcact ttttattgcg 123000tctaattgat tcttctcttc tttttttttt cttttttttt aattgatcat tcttgggtgt 123060ttctcgcaga gggggatttg gcagggtcat aggacaatag tggagggaag gtcagcagat 123120aaacaagtca acaaaggtct ctggttttcc taggcagagg accctgcggc catccgcagt 123180gtttgtgtcc ctgggtactt gagattaggg agtggtgatg actcttaagg agcctgctgc 123240cttcaagcat ctgtttaaca aagcacatct tgcactgccc ttaatccatt taaccctgag 123300tggacacagc acatgtttca gagagcacag ggttgggggt aaggtcatag atcaacagga 123360tcccaaggca gaagaatttt tcttagtaca gaacaaaatg aaaagtctcc catgtctacc 123420tctttctaca cagacacagc aaccatccga tttctcaatc ttttccccac ctttccccct 123480tttctattcc acaaaaccac cattgtcatc atggcccgtt ctcaatgagc tgttggctac 123540acctcccaga tggggtggtg gccaggcaga ggggctcctc acttcccagt aggggtggcc 123600aggcagaggc acccctcacc tcccggacgg ggcagctggc cgggcggggg gctgaccccc 123660ccacctccct ctcggacggg gcggctggcc gggcgggggg ctgaacccct cccctcccgg 123720acggggcggc tggccgggcg gggggctgac cgccccccac ctccctcccg gacagggtgg 123780ctgccgggcg gagacgctcc tcacttccca gacggggcgg ctgccgggcg gaggggctcc 123840tcacttctca gaaggggcgg ctgccgggcg gaggggctcc tcacttctca gatggagggg 123900ctcctcactt ctcagatggg gcggctgccg ggcggagggt ctcctcactt ctcagacagg 123960gcggccgggc agaggcgctc ctcacctccc agacagggtg gtggggcagt ggtgctcccc 124020acatctcaga tgatgggcgg ctgggcagag acgctcctca cttcatccca gacgatgggc 124080ggccaggcag agacgctcct cacttcctag acaggatggc ggccgggcag agacgctcct 124140cactttccag actgggcagc caggcagagg ggctccttac atcccagacg atgggtggcc 124200aggcagagac gctcctcact tcccagacgg ggtggcggcc gggcagaggc tgcaatctcg 124260gcactttggg aggccaaggc aggcggctgg gaggcggagg ttgtagccga gccgagatca 124320cgccactgca ccccagcctg ggcaccattg agcactgagt gaatgagact ccgtctgcta 124380tcccggcacc tctgggaggc cgaggctggc ggatcactcg cggttaggat ctggatatta 124440gcctgttcta cacagtgaat ctccgtcttt accattttat tatgttatat tagttctgcg 124500tggtggtgcg cacctgtctc tctcatgcac tcggctatgc tgtagcatga caatccgtca 124560gtgttgttgt ttcgactctt gagtcgcctc gacactcagt cttggtgctc cttatcatat 124620ggagatcgtt ggtcgtcgtt tcttgtttgg gttttgcttg tgccttttct gtttattgta 124680tcgcttatga tcgtgatgtt gtcttagttg cttgttggtc tctagtgtgt tttttgtgtt 124740gtatcttgtt cttgcgttga gtgggttggg cgttggcccg aggctttctt ttggttctgt 124800ggtgtctctg acgttttgtt gctctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 124860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 124920nnnnntagat ttggacgtta tgttttgagt attttatttg attgggtatg gttagtagta 124980ttgaggggca tgtgggttaa ttaggttttg tatagattat atttatttta tttattgtat 125040tttaatgatt agatgttggt tttaacgtga tagatggtgg gtatagatta tagatgtgag 125100tcggtgagct atatgggata ggtaggttgg gtgtgtcgtg cttctcgcgg cctctctcag 125160cagtgtgctt cggcctcgag accaccaact caacatcacg tccatctcat ttcgcgctac 125220gacacactca tgtctgtacg cacgagtgta tcttctcgcg agcgcttctg tctggcgtgc 125280tgggggggcg tggggggggg gggcggggcg gggggagata ggacgtggaa gaggagagat 125340ggcagagggg agatggcaga gggagagctg aatcttctct tttcttcttt attagtcttg 125400ctagcagtct atcaattttg ttgatccttt caaaaaatca gctcctggac tcattgattt 125460tttgaaggga tttttgtgtc tctacctcct tcagttctgc tctgatctta attatttctt 125520gccttctgct agcttttgaa tgtgtttgct cttgcttctc tagttctttt aactgtgatg 125580ttagggtgtc aattttagat cttccctgct ttctcttgtg ggcatgtagt gctataaatt 125640tccctctaca cactgcttta aatgtgtccc agagattctg gtatgttgtg tctttgttct 125700cattggtttc aaagaaaatc tttatttctg ccttcatttc gttatgtacc cagtagtcat 125760tcaggtgcag gttgttcagt gtccatgtag ttgagaggtt ttgagtgagt ttcttaatct 125820tgagttctag tttgattgca ctgtggtctg agagacagtt tgttatggtt tctgttcttt 125880tacatttgct aaggagtgct ttacttccaa ctatgtggtc aatttcagaa taggtgtgat 125940gcagtgctga gaagaatgta cattctgttg atttggggtg gagagttctg tagatgtcta 126000ttagctccgc ttgctggaga gctgagttca attcctggat atccttgtta actttctgtc 126060tcattgatct gtctaatgtt gacagtgggg tgttaaagtc tctcattatt attgtgtggg 126120agtctaggtc tctttgtagg tttttaagaa cttgcttaat aaatttgggt gctcctgtat 126180tgggtgcata tacgtttcgg atagttagct cttcttgttg aattgatccc tttaccatta 126240tgtaatggcc ttctttgtct cttttgatct ttgttggttt aaagtctgtt ttatcagaga 126300ctaggattgc aacccctgcc tttgttttcc atttgcttgg tagatcttcc tccaatccct 126360ttattttgag cttatgtgtg tctccgcaca tgagatgggt ttcctgaata cagcacactg 126420atgggtcttg actctttatc caatttgcca gtctgtgtct tttaattgga gcatttagcc 126480catttacatt tatggttaat attgttatgt gtgaatttga tcttgtcttt acgatgttag 126540atggttattt tgctcattag ttgatgcagt ttcttcctag cattgatggt ctttacaatg 126600tggcatgttt ttgcagtggc tggtaccagt tgttcatttc catgtttagt gcttccttca 126660ggagttcttt tagggcaggc ctggtggtga caaaatctct cagcatttgc ttgtctgtaa 126720aggattttat ttctccttca cttatgaagc ttagtttggc tggatatgaa attctgggtt 126780gaaaattctt ttctttaaga atgttgaata ttggccccca gtctcttctg gcttgtaggg 126840tttctgccga gagatcagct gttagtctga tgggcttccc tttgtgggta acctgacctt 126900tctctctggc tgcccttaac atcttttcct tcatttcaac tttggtgaat ctgacaatta 126960tgtgtctcgg agttgctctt ctcaaggagt atctttgtgg tgttctctgt atttcctgaa 127020tttgaatgtt ggcctgcctt gctagggtga gcaagttctc ctgcataata tcctgcagag 127080tgttttccaa cctggttcca ttctccccat cactttcagg tacaccaatc agatgtagat 127140ttggtctttt cacatagtcc catatttctt ggaggcttcg ttcatttctt tttattcttt 127200tttctctaaa cttatcttct cgcttcattt cattcatttg atcttcagtc actgataccc 127260tttcttacag ttgatcgaat cggctactga agcttgtgca ttcgtcacat agttctcgtg 127320ccatgttttt cagctccatc aggtcattta aggacttctc tacattggtt attctagtta 127380gccattcgtc taatcttttt tcaaggtttt tagctttttt tgcgatgggt tccaacttcc 127440ttctttagct gggagaagtt tgatcatctg aagccttctt ctctcaactc gtcaaagtca 127500ttctccatcc agctttgttc caccactcgc gaggagctgc cttcctttgg aaggggagag 127560gcactctgat ttgtagaatg ttcagctttt ctgctctgtt tttccccatc tttgtggttt 127620tatttacctt tggtctttga tgatggtgac gtacagatgg ggttttggtg gtgtggatgt 127680cctttctgtt tgttagtttt ccttctaaca gtcaggaccc tcagctgcag gtttgttgat 127740gtttgctaga ggtccactcc agaccctgtt tgcccgagta tcagcagcag aggctgcaga 127800acagcgaata ttgctgaaca gcaaatgttg ctgcctgatt gctcctctgg aagctttgtc 127860tcagaggtgt acctggccat gtggggtgtc agtctgcccc tactggaggg tgcctcccag 127920ttaggctact ctggggtcag ggagccactt gagggggcag tctgtccctt ctcagatctc 127980aaattccatg ttgggtgaac cactagtcac ttcaaagctg tcagacaggg acatttaagt 128040ctgcagaggt ttctgctgcc ttttgttcgg ctatgacctg cccccagagg tggagtctac 128100agaggctggc aggccttctg agctgcgatg ggctccaccc agttcgagct tgctggcaat 128160gagcaaggct ctgtgggcat gggaccctcc gagccagaca tgggatataa tctcctggta 128220tgctgtttgc taagactgtt ggaaaagtac agtattaggg tgggagtgac ccaattttcc 128280aggtgctgtc cgtcaccact tcccttggct aggaaaggga attccctgac tccttgcact 128340tcccaggtga ggtgatgctc acactcaatg ggctgccccc actgttctgc ccccactgtc 128400caacaatccc cagtgagatg aacccagtgc ctcagttgga aatgcagaaa tcacccatct 128460tctgcattgc tcatgttggg agctgtagac tggagctgtt cctattcggc catcttggaa 128520ccagtctgaa ttagtgaatt ttcaacataa aactcaactc ctgtgctctt agccactcac 128580tttacaaaag ccaaatccag ttcctcatcc tcttgcccac tcactcagca agccttgggg 128640cccctgcctt tcagtgctta gagaatcatg ttcagatgca agagccaggc ttggttctca 128700ctgggcttgg caggcacagt acatgcctaa ctctggcctg cgtgtgcatt tccacttgag 128760gtcctaacct tccactcagc ccctcaagac tcttcaggga cctaagggcc cctggtgcag 128820agctgccttt ttgctccagg agaaaggcat ttcgtggccc ttggtgggta tctttaaatc 128880actgcagaat agtttactcc tcttctctga aattcttata ctgagcaaaa acgggagttc 128940actctgggtt ttagtgttgc ctgtgagagc agcagttctc acagctctta tgtttctaag 129000tccatttcat tgtttcatgg agaaatgtgt gacgatgaca caacccggtt tcctgtcgtg 129060cctacacaga ggattgagaa tgagctccat tctaaaagag tgcagtgtcc tagatggctt 129120tagaagagac acatgtgctt ggacttgggc tctgtcactg aagctgtgtg accaagagtt 129180gccctgaccc cctcacatgt aaaatggaga tgataatgcc tacttctccg ggtcatggtg 129240tgcatttggg gagagaatgc aggaaagccc tccacacggc gcctggcacg cagtaaatgc 129300caatggatgg gaacaatgat cattatcatt atgcctatag tcatcactgg cctgagggtt 129360ctgctcctcc ctctaagcct agctctgaga gcatcttatc tctcagcctg tcctgctgcc 129420gtctctcact gcaggagttg gagcaggtag caggtagcta agggcggggc tgcttctcag 129480agcctttaga gcagatgagg tcatatcatg accactcttt aaactattgc ttaaggccaa 129540gcctgtgaac gcagcccata gagctgtgaa tagggggagc ccagatctcc tgaaaacagg 129600tcctggaata gtttctctag ctctcgtggt aaagcattgt gttttaaaac ttacttttca 129660acacagatta ttatattttt gtaaaatata ataaaaatta attactagaa aaatgaaatt 129720ttaaaaaccc aaagatattc aaaatccaag tctttttttt tcttattagg tgcaaaagtc 129780acaaaattgt tctgttaagt tgctataaaa gtttctaaaa cccctcctct gagtctttgt 129840attttatttt gtatttattt tatttttatt ttttttaaga gacagggtct ccctctgtct 129900cccaggctag agtgcagtgg catgatcata gctccctgca gccttgaact tcaggactca 129960agtgattccc caacctcagc ctcctaagta gttgggacta taggcacaca ccaccacacc 130020tggctaattt tttttttttt tttgagacag agtcttgctc tgttgcccag gctggggtac 130080agtggcatga tctcagctca ctgcaagctc cacttcccag gttcacgcca ttctcctgcc 130140tcagcctccc aagtagctgg gactacagtt gcccaccacc acatccagct aattttttgt 130200atttttagta gagacggggt tttactgtgt tagccaggat ggtctcgatc tcctgacctt 130260gtgatccacc cgcctcggcc cctagctaat tttcaatttt ttttgtagag atggggtctc 130320tctacattgc ccaggcttgt gacaaaatcc tagactcaaa caatcctccc gccttagcca 130380cccaaagtgc tggaactata ggcttcaacc tctgtactga tctaattgca gacctggacg 130440cagttagttc tcagatgaca ctcaggccac attctgagct gtccacccga cagcacttcc 130500aggctgagac atcttcatcc ttgagcttgg aactggggtc caaaaaggaa tattaacggg 130560atacctctgc atcctcccct ggggatgtaa agccagccag gaggggaggc ttgaggccac 130620agaaggcaag ctgagtatta atggtgacaa taactgttat cattggctat gggggtaatt 130680ctggaggcac tcaacattca tcatctctta tctaccttaa ggaaccagct aatctcttgg 130740gcaccttgac tctgaggagc tggatctctt cagtttcact ggtatgaata agtcagtaca 130800tgcggaattg caacatgcca agtttcaaaa agagaatagt tctgtctccc ctggtcccga 130860aggtgtttga agaatttatc caatggctga tgaagttctg ggagcacagc aggatgagaa 130920agcctggact gacggcagaa gagctcagca gggcaggttc caaggactga cacagtgcct 130980taatgaagct gtcccttcac acagatgctc tggatctgtg gggccacagt aatggccctc 131040tgacattcat gttcacccaa ggtgtagtgg tgaaatatta gatttaaggg gtgatcatat 131100ggtgagtaat tgaatcactt gtaggctggc ccccctcatt tccaggttgt tcaagggatt 131160ggttgttttg ccatgtggtg gattaataat tggccacagc tggacatggt agctcatgac 131220tgtaatccca gcactttggg aggccgagcc gggtggatca cctgaggtca ggagttccag 131280actagcctgg ccaacatggc aaaacccttt ctctagtaaa aatacaaaaa ttagccaggc 131340ttggtggcta cctggagcct gtaaaccaag ctccctagga ggctgaggca ggagaatcgc 131400ttgaacctgg gaggcagagg ttgcagtggg ctgagatcgt gccactgccc tccagcttgg 131460gcaatagagt gagactctgt cccaaaataa taataataat aataataata ggccacaaat 131520tctttgacat tccttccatt gagagatggg ggggtctgca tccccacccc cacccccgga 131580gtctgggtgg gctctgtgac tgcttggacc agtagaatat gatggaagtt acagtgtgcc 131640agtttccagg cccaagcctg aagagattgg cagcttccac tttctgtctc ttagaaaact 131700tgctcttgga agccagccat catgtaagaa atgcaggcac ccagaggaca ccattctgtg 131760agaagcctta aagaatgaga ctccatatgg acagagaggc caaggagcct agaggtgcca 131820gacacatgac agaagaaggc ccagccccct tcagctgatg ctacatggcc acagatgaac 131880tgcccagtga agcctttcct aaattcctga cctgcaagag catgaacaaa attaaatggc 131940tgtttaaagt taccaaggag gttggtttta cagcagtaga gagaatggaa cttgctggac 132000agcttctcta tgctcaccct ccaacctgtg ctgtccctga aagtgattgg aatgagatgc 132060ctcaatgggt tttccactgg gaactctgac cagaaaggag ggaatgtgta gaggaaggag 132120aggccaatca ctccccagaa cctttctact gagggccgca gctcctccaa gggtgctgtc 132180tccaggatac ttctgcatta ctcccacaac cttccccctc cccctgcatc aggactagtg 132240gtgctgatgg ccccgcactc tgcatggtcc ctctgcacat gggcccacac ctttgtgatg 132300cccctttgtt aaaccctcat cctagtgtac catctgtttt ttgccaagat gagctgaagg 132360gtctgcagga agaagggctt cataaccgag accttgggta gatgtgcagg agggagtttc 132420agagccagca ttttctaact accagaaaaa gagaagtgcc ctgtccacaa ccttccacat 132480tctgttgacc acaggaataa atgaactttg tgattttttt tcctgtcatt aggtaggggg 132540acaaaaatcc tcttaactcc atgttcagaa atattctgga aatttggttt cttaagtcaa 132600attccacatc agaaacgatg ggatgtggcc tggtagctta agaacagcag gtacagaata 132660acttttttct cctttgtctc agttttctta gctataaaat gggggtaaca gtcatctctt 132720cctacctacc ccaccaaact tcgtgaagaa cacactagct acaggaaact tggcaaagag 132780aaagagtttg acgctgtcat ggcagaagca gtggctttct cttattacgg tggctgcctg 132840gcctttgaag gcttatcttt ccaggtttaa actgagagca agctgaaggg ttatgactcg 132900aagtgaggta ggattttgcc aggcacacta ggaggctggg tatgcaaggt ggttcattca 132960gggagccttg gctgaccttg acgcctgcgt ccgtctctga tgctctgtac caggtcagct 133020gtgtgtccct aacaaagaat ggcctcctaa ctggggtctg cacatctggg catcttagta 133080aggggaacag atttgaggtt cttctttctt tcagcgaaag gtgagacttc gggttgaaga 133140actggtaact gtgatttgca ggcttgtcca tggctcctca gagctggcac ttggggtgct 133200ccttgtccac cccttccctc cctaggccca catggggcac cctgagcttg cctgagccct 133260actgtgatct cacggagggc aggctatgaa ctagaaagtg atgccacacc ctggcaacta 133320gagaagtgcc catccagggg catgtcccat aggtatctgc ctgtatggac cctagttacc 133380cactgacaaa aagaggggca caaccctccc ctgtagaact gtagcgcaga gtgaggacat 133440gacgacagtg aggcctgggg ccacccgctc caccctcagg ggcttgtaag aatcccaagg 133500aaagtcatgc ctcctgcagg tgtgtcgagg cacagggcag agccctccca cttcccgccc 133560ctgcacagca gtgtcattcc tcctatgact acagtgcttt gaggacagga agaactttcg 133620gttcttggga agcccggggc agaaggggcc tgagctgctc accgcgtcca tccctcagga 133680caacctcgac atcgccggtg atccagcggt agcaggggaa ctcgatgtag tccccgtggg 133740gcgtcttcag cgtgatgtac ttcaggtacc agtcgtcatt cagccagtac ttgcgcttct 133800cgattctgac cagctggatc tcgcccagtt cctcgtccac agtcacgtcg tatgaatcca 133860cctgggaagg agaacaggca atcaggccat gcgtggtctc ctgagccttt gttctggagg 133920tgttaagaat ttgtcacccc aaggtatgct gctgtggcac agggactacg ttgaatgaaa 133980agcacttaaa aaaacagcag gtgcaagata ctctgacatt cgtgctgttt cttagaagca 134040ggagatgaaa gtcccctatg aaagatgtcc tcactatacc aaaaggaaag taacactctt 134100ttcatcaagg ttgggaagtt gaagccaaag gaaatccata caaacagatc ttgtacaatg 134160aacgcttacc tcccgagtca cttccctgcc cagttacctg ccctagccca agccccttgg 134220ccttatcaca tctcacgatg attcattcaa tgcagtatct aagggggatt gactctaact 134280gcttcttggg gtcttcatct ttttaagagg gctcccatgc cacgtacaac ttgtattaaa 134340gaaatctgta ggctttcgcc catgaatctg tcttatgccg attctcagac ctagctgaaa 134400tgggaagagg tggagttttg cctccactac agttcatgga gccaagattc ttcatgaaca 134460ccttaaatga actcctgaat tcatttaccc aagacattca ctgaaggcct gcccctgaca 134520aggctcagtg ctacgaccca ggcaggtgaa agaaatggac aaatcctggg ccctgccctt 134580gaggagagag aatccagtga gccaggatgg tgctgtgctg ctcagagggc aacgcctgct 134640gactgcactt agctcaggct ggaggatggg gagatgccca ggctcatgcc tgcacctgcg 134700aggctgtgcc cagtgagggt ccgcagctgc ccccacatgg gatagccttg tgggagacct 134760ttacaactgg agatgggctt gctgacagtg gatccccaca atgactctgg gagtgcaggg 134820gagttaccac cccttccaca gatgaagaca gcgaggctca gtgactccca cctctgccac 134880ttgccagcag agctggagtc acaccccagg tctttccatc atagccactt cctctctttc 134940cagttttcag tggggataat aagtgagtga aaatggtgag gcttagatac agggagcttg 135000catggattcg agtcgtcctg ggacatttgc agttcacagc tgcatggtga tgcccagaga 135060acagtgagac caagcagctc tgctgcacct gtggcattac tggtggcagg gtgaggctag 135120ccttgcagct agaaggcacc accccaggct gtgtacagcg cagctgccca ggcctctggg 135180acaaggctgc ttggccccct ccctgccagg ctcctgcttc caggacacag gcggctctga 135240ggcaacagcc atagcttctg gagggctttt gtctctgcag caatggatgg cagacaggaa 135300gttgggatga gaggctcagg cattgcatca cgcattctga tgaccagcag gtttggaaag 135360ccccggactc gatgccctcc aaggcatgtt cttttttatt ttgaggtgga gtctcactgt 135420gttgctcagg ctggagtgca gtggctcgat ctcggctcac tgcaacctcc acttccaggg 135480ttcaagtgat tctcctgcct cagtctccca agtagctggg actacaggca cctgccacca 135540catctggtta agttttgtat tttcagtaga gacagggttt caccatgttg gccaggctgg 135600tctcaaactc ctgacctcaa gtgatccacc tgcctcggtc tcccaaagtg ctgggattac 135660aggcatgagc cactgtgcct ggcccctgca aggcactttc taagccagat tcatcaaccc 135720ttgaacttta tccctggcgg cagaacctag agttctcagc atgatggcaa agtggaaggg 135780gcgaggggta tcatcctttg tgcgagcgca ggcatcccca ttccatgccc tgcctccaag 135840ctgccaggag gacccctgga gggcttccta agaggaatgg gaacttgagt gttttccaca 135900aacctcctgg ctctgaatac cgtgagcagt ttggggtggc tgacgctgag gccagacgct 135960tgtggtcaga aagcggaagc ttcctccctg tgctctgtag tctaggccag aggccctagg 136020aacagtttct gatgacagag gagccaggcc catgtggtgg gatgacatgt cttgggtcca 136080gccacatgtg gaccagcctc ggccccacga caatgcccct cctgcaggga gagtatcagc 136140ctcatctgcc acctctgaga aagccttcct gccacagctt gtcctcagga gagcagggcc 136200acacacaagg cctcgcccat catggacagg gtgggcaggt tctgatggga caggagtctc 136260agcatctcag gggctcgggc ccatctggag gagcagagtg ggggctgagt ggcccaggac 136320tggatacgcg tcgggcctgc cccacgccag gccctgcatg ccacagggat ttacagaggc 136380cttagctgca ggttgtgagc caagacagag attcctgatc ccaccgagga gtggggagag 136440ggcagtgagg ggtagaaggc tcggtgggcc ctggggtctg gagctctgaa ttcacatccc 136500agcttcaggc aagtagctgg atggcctggg cacattgctt cattctctga agctgtttct 136560tcatctaccc aaaggagata cccctcactt gcaggaccat tgagaaaatg aaatgacacc 136620atcctgggtg gcctcctttc tccagaggca caggccaccg gttgtgtgca tcagaaccac 136680cagtgcccct tcctgaatcc agggggttgc agggcaccag gccccacacc gcctgtgagc 136740tagcatgggc ccctgcgtca cggccacttc gtgactacat gtgccaatcc gagtaactgg 136800agcacacatg ggaggaggaa actgaacctg tgacctcaaa ctccttcact caaagcccag 136860aaggaactcc tgaaaggctg gagacagggc tgtgaggaca gagggagagt gggaggcatt 136920ctgaatgctg gtggggcagg cagagcaagt ctgtgtgcgg tgaggactga ggggcggcca 136980ccagaggccc gggcctggtc tgagtgtggc tgctgcaggg gaagtagctt tcctatggct 137040ctgaggggct ggagagggag gaaggtgtcc ccaggggata gatgacagga aagctggggt 137100ggggtgggag gagctaacag ggacactctc atgggaggga ggctcccagt accttggact 137160aagaggggag ggagatgggc tgaggaggca ttccaggact gggcaggtcc cacagggagg 137220gcccgatgtc tacctggggg tgacgaccca cacgaagaga ctgagcagct tctctgaggg 137280agtggacagt ggggtgaccc tccagcacac ctgtggtgca gtcacagggg actgggacac 137340tcctggcccc ctgccctacc tacacctgca ggcacctggt ggagagcccc tgagtttcac 137400ttggggtgaa atcactaggc agatttacaa ggactccatc tgccatctga cagggacttc 137460cacagcagga gggggccagg cctgcagtct ggctggtcta tggccagccc agccactttc 137520ctggtgctgg cttcaagcct gaatatgata agaggtgaac aaggggcaga gggctccagc 137580cttgacccaa ctcagggagt aaaagacaaa gcttgatggc tgtggttcta acatcccagg 137640ggccactaat ggaagaatga cagataaagg cctccttcca ggaaaatacc agggtcacat 137700gtgtcaaact gcaaaatatc ttcggaaggt ttgtggaact acaatggcca ccttcctccc 137760tcctctctgg gccaagtaca ctgggctgat gctatatttg tctatttgct gtgacacatt 137820aaattctcag gtgacacact gaattatggt gggcactatc aacagtggac accccccaac 137880aggccctcag gaagcactgg gcctgggaat ggccgagcga gtgattatcc tgctttacag 137940atagaaactg aggctcagag agcaatggtg gcctcaagat cagaacctac acctgtgtga 138000tggccaagcc caggttcctc ccacagttcc ccaaggccac atcttgagaa aagccctgaa 138060ccggaagcta gaaggcccag ctttgagccc tggtttgtac cctggatgga gtgtaaaggg 138120gcaatgtgga gtggcatgga aggacaggga aaagcttcat aagagggtat ttcaggcagc 138180ttttaggggc tgatcagatt tcagagggca gatgagtagg aggatgggca tgggtagcac 138240atttcagatg tggggaactg ggagggcagg gtccccgggg gattcacacg tgggaggagg 138300aaggctggac acagtttctg gggacagaag tgggtggcgt ggtggtgcag atgctggaga 138360gaaacaaggg aaacctgcaa ctcaccggcc tcatttcaca aggggccact ttgctgattt 138420tgcttgttgc ttttaacttt ttattggata tgtagtttta aagcttttga atatttcggc 138480aaagagtcag ttggtcagtt ttcatgtgag ctttctatca gcattttagg aaagttttca 138540tctgtctttc atcagatctc tgctgttgct gggacaggga gacggatctc tctgcggaag 138600caaggatgtg tgccccaccc ccagactacc catcttggag ggggactaat gaggggctgt 138660gggaggagga agctgtagag cagagaggaa gattgggggc cagacaaaac agagactcgg 138720gatgagagag ggggaggcta tggggaagag ctgaggaaaa caagggacgg agagagggat 138780tggggtagtg gatagggaga ccaaggggca gagaaggggg aaaatgtggg acagagagcc 138840ggagattgag ggagcagaga aggggcctct gagaggtggc tggaagacag agtgtagagg 138900aaaattcaga gcaaaaaaga agggagagtt gtagggtaga gagcagaaac ggggggcatt 138960tagggaactg ggagcagggg aaggaagagg aggcagaaga gggggcagaa tgtagggaaa 139020agatggctca gagggggaga ctgggagatg agagacggat tgagggtcat accaaagaaa 139080tatttatgct ccactaatat attcccattc acaagaagga ggtattctag aattaatttt 139140tcatgaatgt aagaatactc actgacttaa gaagcatgaa tcattataac acttttctac 139200acttgttgaa catattcaag tatcatatat taaacaccac gctattgccc ttagctttct 139260taaaagtacc agcacagaat gaaatgggcc aactaagatt tctcagaaat cttcagatct 139320gttacagtta tcgtatccga tctgaattac tttaaattta cagagttaaa gaaataaatc 139380aacttgttaa atttgcctag acttgggtta gcctctggaa acagggccat tctgtgtagg 139440atcccggctt tctccagcag acagtgagga gccaaggaag acacaaccgt ttataggata 139500atcccacctg cgaaggactt gattcccagt cctgactgaa tcactatgcg gaactctgat 139560gagaataaca agggacctgt gactcacttt atcaaggaaa aaatcagtcc tgtccattat 139620ttgactgaac tatgccctgg aggagggggg tgttgacctg ccaataactt ggaaatgatt 139680aaattgtgtg gaaatacctt tattgttatt gttatcgtta ctactgttaa caatacagat 139740tttaacactt ttccctgtaa aatgggataa ccttgtcttc tcagcaagta cttcctctag 139800ggtttctctt ttacctttac ccaaaaagga tgattgtctt tacagatacc aagctgggag 139860aatgaggaag tagttctggg ctgggcaaga gaaagtgttg agtaactttg catcaacagg 139920aactttctca aaagctgatt ttttcaagga aactttcagc cttagctggt tccccaccct 139980gcggtagccc ccacccccaa ccaccccccg ccccacccct gacttatagc cctctcaacc 140040caatggaaat gtgacatgca aatctctcct gaaatttgcc cctgggattc cggctgaggt 140100gtttaattat aatgtcattt gaatgacttc tcagggtagg ggaagacaga ccgagagagg 140160gagatagtgg acaaaacagt gccttcactc tttgtccgaa gagcccgcta tggccacgtt 140220atcatgtccc ctgcaggagg aaagcacaca cggggtgccc gtgctgtgca gtgtgagcac 140280accaatggcc tgtgcttcta gaaccttatg atgtgtggag gagaatgggg aggaggaagc 140340acttacaaag gggaagtagc ctgtaccctg tccttctccc ccaggtgtgg ggaatccatg 140400aaataatgga agtaaatgaa ggtgcgtcag aaatggcaga gggtgccatc accctaaatg 140460gacgacacat taactccagg gcaccgcagt cctcagggtc agttccccga ctggggcaac 140520taagcaactg aaggttgctc ttgtactcaa cacctaactc ttcttgaagg gagctcaagc 140580caggatgcta gtgaacttgg ctgagggcca tgtcgtgcta cagcgagggt atgggctcct 140640ggataggaat cgagcccatg gagagcttgg aatccagaaa aagagtagtt tttacttact 140700ttaggctctg cacacctaaa acacaaattt tatccaagag taatcagttg aaacatgaac 140760ttgccggttg tatttgtcca ttatgagcaa tttcacaagg tttgatcaaa taataaacta 140820aatggtgaga aggcagcttg gtaaagtcta aatctttttg gtccagttag gagctggggt 140880ggggtggaag ggaactgata ttccctgcgc acctgcagta tgtctgggac tattaccagt 140940catttacgga gcgcttcctc tgtgccaggt actggattag gcacttcatt aaaggctcag 141000tgagaactgc gacctgccca tgctcatgga aaagagtcgt ggggctggaa ttcaggtcca 141060gatcagcatt aggaactagc ttccaaatgc tgagggctcc ctcgcgtcct cacacctagc 141120gtggaagtgt tccctggtgt acattctgct gggagggagt gcagcccctc catttaggaa 141180aagcctctcc tggcctgggt tctgttcctt ccctttaatt tcctctgggg actgtcctga 141240gaatgtaagc tgcccaggtg ccgtctgagc caccagatgg gcctgggctc acccatgact 141300catggaccag actgaagcca accctccatt ttcagctggg gaaactgagg cccagacaga 141360cggtagagtg agctggtggt catgtctgga cagaactcag gcctctggac ccgctgacgt 141420gcacccttcc ccacggctca cagctggctg ctccgaggtg ccctcagagt tctgtgagca 141480tgactcatct gcctctccct ccctgtgcga cctgcagaag gagcctaagg aagaggtggc 141540acaagaaacg cggcccagtg aagggggctc cggagcctcg gagcccagat cttgaagtgg 141600aggggaaacc ttgggccttc tcctcccagg gaagacgcct tgcaggcggc acgcgggaag 141660tctccgtttc cgacaggggt cgcggccgcc gcgaggggtc ctggacgctt gcccaccagg 141720gtacccaccg gccactggga gcccagggca cgccccaccg cagttctgcc ctccctgaca 141780gggtcctgga cacccccagt cctgtccggg ccgccccgac gggccctcgg cgcccccgcc 141840caggcctctg ccgtccaaac cgggtcccgg acgcacctca gcccgcgctc cacccgtgcc 141900ccgcccgcgc tcaccgcgcc acgctcgaag tcgttgtaga agggcttgtc cagcaggtgc 141960ttctcgctgc agcccgccga gcccacgagg ctgaggtaga tgtagtcgtc agtgtcttca 142020ccacggggtg aagtacggtc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142140ntttttgggt tgttgttgtc tctttgtttt tgcgagcggt ccttccgccc cgtggtgaag 142200acaagtgggc gcctctgcct ggccatccca tctgggaggt gaggagtgcc tctgcccggc 142260caccccgtct gggaagtgag gagcgtgtct acccagccac cacgtctggg aggtgaggag 142320cgcttctgcc tggccgcccc atctgggaag tgaggagcgc ctctatctgg ccaccccttc 142380tgggaagtga ggagtgcctc tgcccggccg ccaccccgcc tcggaggcga ggagcacctc 142440tgcccggcca ccaccccatc tgagaggcaa ggaccacctc tgcctggccg ccatcctgtc 142500tgggaggcga ggagcacctc tgcccagccg ccgccccacc taggaggtga ggagcgcctc 142560tggccagccg ccccacctgg gaggcgagga gcgcctctgc ctggccgccc catctgggag 142620gtgaggagcg cctctgccca gccaccaccc catctgggaa gtgaggagcg tctctgccag 142680gccgccccgt ctgggaagtg tacccaacag ctccgaagag acagcgacca ttgagaacgg 142740gccatgatga cgatggcggt tttgtcaaaa agaaaagggg gaaatgtggg gaaaagaaag 142800agagatcaga ttgttactgt gtctgtgtag aaagaaggtg acataggaga caccattttg 142860ttctgtacta agaaaaattc ttctgccttg ggatgctgtt aatgtacaac cttaccccca 142920accccgtgct cgctgaaaca tgtgctgtgt caactcaggg ttaaatggat taagggtggt 142980gcaagatgtg ctttgttaaa cagatgcttg aaggcagcac gctcattaag agtcatcacc 143040actccctaat ctcaagtacc cagggacaca aacactgctg aaggctgcag ggacctctgc 143100ctaggaaaac cagagacctt tgttcacctg tttatctgct gaccttctct ccactattat 143160actatgactc tgccacatcc ccctctctga gaaacaccca agaatgatca ataaatacta 143220aaaaaacaaa ttaattaatt aaataaattt catatggaac caaaaaagag cctgcattgc 143280caagtcaatc ttaagccaaa ggaacaaagc tggaggcatc acactacctg acttcaaact 143340atactacaag gctacagtaa ccaaaacagc acggtactgg taccaaaaca gaggtataga 143400ccaatggaac agaacagagc cttcagtaat aatgccacat atgtacaact atctgatctt 143460tgacaaacct gacaaaaaca agaaatgggg aaaggattcc ctatttcata aatggtgttg 143520ggaaaactgg ctagccaata aatggtgtag ggaaaactgg ctagccatat gtagaaagct 143580gaaactggat cccttcctta catcttatac aaaaattaat tcaagatgga ttaaagactt 143640acatgttaga cctaaaacca taaaaaccct agaagaaaac ctaggcaata ccattcagga 143700cataggcatg ggcaaggact tcatgcctaa aacaccaaaa gcaatggcaa tgaaagccaa 143760aattgacaaa tgggatctaa ttaaactaaa gagcttctgc acagcaaaag aaactaccat 143820cagagtgaac aggcaaccca cagaatggga gaaaattttt gcaatctact catctgacaa 143880agggctaata tccagactct acaatgaact caaacaaatt tacaagaaaa aaacaaacaa 143940ccccatcaaa atgtgggcca aggaaaagaa cagacacttc tcaaaagaag gcatttatgc 144000agccaaaaga cacatgaaaa aatgctcatc atcactggcc atcagagaaa tgcaaatcaa 144060aaccacaatg agataccatc tcacaccagt tagaatggtg atcgttaaaa agtcaggaaa 144120caacaggtgc tggagaggat atggagaaat aggaatactt ttacactgtt ggtgggactg 144180taaactagtt caaccattgt ggaagtcagt gtggcgattc ctcagggatc tagaactaga 144240aataccattt gacccagcaa tcccattact gggtatatac ccaaagtatt ataaatcatg 144300ctgctataaa gacacatgca cacatatgtt tattgtggca ctattcacaa gagcaaagac 144360ttggaaccaa cccaaatgtc caacaatgat agactggatt aagaaaatgt ggcacttata 144420caccatggaa taccaagcag ccataaaaaa tgatgagttc atgtcctttg tagggacatg 144480gatgaagcta gaaaccatca ttctcagcaa actattgcaa ggacaaaaaa ccaaacactg 144540catgttctca ctcataggtg ggaattgaac aatgagaaca catggacaca ggaaggggaa 144600catcacacac tggagcctgc tgtggggtgg ggggttgggg gagggatagc attaggagat 144660atacctaatg ctaaatgacg agttaatggg tgcagcacac caacatggca catgtataca 144720tatgtaacta acctgcatgt tgtgcacatg taccctaaaa cctaaagtat aataataaaa 144780aaataagctt ataaaatgct tttaatgcta tataacttta taactctgaa agaataatag 144840atttgtatta tgctgtcagt gagtaggtct attaagtgat aacattaaac acaaatacca 144900ccatttataa agtatgattt tgatatcatc ggttgtgtat tttcacccca aactctgtag 144960atagtaactt tgttttctca tttgaccatg ctcaatcttt agagaccatg ctcttctctc 145020aaaatgactg taggttcatc caagtatgtt tgctacccaa aatcaatgaa acacagtcac 145080aactatgtct gtctaggaat aaagccagct aattagattc ttattctaag gaacaagaag 145140agggcaggta agaatttgat tatggcagaa ttctgaccat aatcaataac tgttttctat 145200tttttctcca tgttgtcggg tggttcatac ctgactttca tcatttccaa tgattcctgt 145260ttgttttgtt tataaatttc aaaatcatca tgaaaagttt cactgaatga aaacctgaaa 145320attgggaaat aaacattttt ctgcaaaaaa aaaaaaacaa tgaacactga cttctcctat 145380ttctcaaagt catctcaaag ccatatgagg attaccacaa acttgcaggt gagaaaattc 145440tttgcaatgt aagaagtgcc atgcaaatta gttgatgcta ttataattag tctcagggat 145500caaaaatgct tgaaccctaa atctaacact tcaaaggaaa gcattatttt tcattcattt 145560tacaagttac acctcccttc ttccccacat ctttgtttag aatctggacc ctgaagttag 145620aggaccaaga ttcaaatccc agcctcatca gtactagcta tgtgatattg ggaatgttac 145680ttgatccctg cttatcttac tttctgcatt tattaaatgg ggatgagaat aatatctacc 145740caaataaggt gcccaatgca gtgcccaaca gcatgaagcc caataaaggt tagttaacat 145800cacatccata attgacacct ttaggtatta gattttcatc atcccagaac attttccttc 145860tttaagcctt tttctttcta agtggcactt tcttggatac tgattgcact cctcacaaac 145920ttgaaacgtt caaacacaca agatgccatt taggagacat tggaacttgg gtttccttct 145980ctgtaaaact gagtaatgac agtcacctcc cagggctgtt gtaagaatga atgagctaga 146040gtatgtaaaa tatcttagca cagagtgctt aagaaattat tagttccagc caagcgtggt 146100ggctcacgcc tgtaatccca gcacttaggg aggccaaggc aggcggctca tctggggtca 146160ggagttcaag accacctggt caacatggtg aaaccccgtc tctactaaaa atacaaaaat 146220tagccaggcg tggtggcatg tgcatgtaat cccagctact caggaggctg agacaggaga 146280atcacttgaa cccaggaggt ggaggttgca gtgagccgag atcgtgccat tgcactccag 146340cctgggcaac aagagtgaaa ctctgtctca aaaaaaaaaa aaaaaaaaag aagaagaaga 146400agaaagaagg aaagaaagag agagagagaa agaaagaaag aaagaaagaa agaaagaaag 146460aaagaaagaa aaagaaagaa agaaaagaaa gaaagaagga aagagacaga aagaaagaag 146520ggaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaag 146580ggaagaaaga aagaaagaaa gaaaaaaaga aagaaagaaa gaaaaagaaa gaaagagaga 146640aagagaaaga aagaaagaaa gaaaggagag acagaacgaa ggaaagaagg aaggaattat 146700ttcccttttt cccacccacg cctcaccaca aaccttgttg tggggataac atgtaaaagt 146760gaacaaaact gagaagatca catcgcatat gcactcagcc tctagtggga gacacaggca 146820ataaacagga aaagttaaaa acagtactag gtagtggtga gtgcaattag cctcccgcag 146880cataaaggga ggagaataac cagtggctct ttcatgcaga gaatcagggt agacctctgg 146940ggagagaccc gcactcagac tgggatggaa ttatttattt tatgcgtgtt cagttaggag 147000tagaggtgct ctgtcgtgat tatctaatct tgggaggaac aatgtcagac gggtttcaag 147060atgagtatga gaattggtct tccttctgaa aaaacaagaa ttgagttcac actaatcaca 147120aatacatcaa gcctatttga gtcttgtgtg tgtcatggat tgaagtgtat ctttccactt 147180agtcaagaag ttccaatgag aagaatgctc cttaacttcc taagtgcaac agagtagttg 147240aactgagcca tggatggtaa atctacttaa gaaagcacat gccatggaaa gatagcattg 147300gctgccctgc ctacttctga tggcacagcc ttgcaggaag ggaaagaagc caagaggcaa 147360aagcctggtc ttgttctgaa aggcacaagg aggtggaaga ttaggggaaa ctcacccaaa 147420aatgactcag ctctgaactc agactcaaga agccacaata agcaacatga atggaactgg 147480agggaaataa accaggcgta gaaacacaaa tgttgcatat tcttactcat gtgggaacta 147540aaaacatgca tttcaaggag aatagaatga tggttatcag agactgggaa cagttgtggg 147600tgggggtgtt aagagaggtt gcttaatggg tacaaacata tagttagata gaaggtataa 147660gttctaatgt ttgacagcag agtaggagaa ctatagttaa cagtaatata ttgtatactc 147720caaaatagct agacaggaag atttgaaatg ttcccaacac atagaaatga caaaagcttg 147780aggtaatgga taccctaaat atcctgactt gatcattaca cagtctatgc gtgtaacgaa 147840ataccatatg taccccataa atatgtacaa atattatgta tcaataaaaa tttttaaaaa 147900gaagctgcaa tcaacatttc atcattatct tttcagcagc atccttacaa agttacagag 147960acatcttaag atgtatccct cttcctctga atcttcctgg gatagaacaa atgaaaacta 148020aacaaggagt gggctgggag gagctgtcaa catccatcct aaggaaaagt ctgttagggc 148080tatgagcttc ctctgtccta gagtagagct gggaaaatag agtcttgaca attgatttct 148140tattctttct ttctctaaat ccccaatgaa tatgtttttc tttttagtgc tcagtggtag 148200acagggctta attctgttta gttatgcaaa caggccagaa gaaactgaaa taggctataa 148260atgtttgaag ctggccaaaa gaagatgtag tcacacaaga taccctctgg aggaaagtga 148320ctgtcaggaa agtcactttc ctgaccagca aaaggaccag cactattttg tcctgctgaa 148380gaagtacatt tgtcctgctg ctgtcatggg gacagtggct ccagggcttc ctgtggacat 148440gccacaagtt ccactccatg agtcaggaga tcatgcggtc agctgggggg aacttagggt 148500tgctctcttg agcttagcct tatgctgcgt gtctgctttg ccaaccacaa aaacgatagt 148560ttcatagcaa gttgcaggct ccctggaaac ttaacctacc tactaggata catttacagc 148620tgcttttctc ctggcatcag gctggagatc aagaaataca taaaacatag tccctgccct 148680cagttagctc agaatatggt gggggaatgg agacagaagc aaatactggt gggacaagtg 148740acgatgggcc aaaatatggg cttaaacaaa ggttgtggga gctcagaaaa ggagcaactc 148800actctgcctg aaagagtcat ggagggctcc acggaaaaag caacatttgt gcaggaggag 148860attagaacac agaggagaag gatagcagca ctccagatga agggaaggaa atgtgccaag 148920atttcatggg aatgagaaga tgtaaactgc tcaggaaaaa ccatggttga agctcttcac 148980agtggctgaa gcatggtgtg attgtcagtt ttgcttgtca acttggtcag gctgtagtac 149040tcaactattc aaccaagcac tgatctaggt attgctgtga aggtattttg tagtcgcaat 149100taacctctac aatcagttga ctctatttaa aggacattat tctgtactat ctgagtggac 149160ctgatccact tcatcccaaa ggtaaatgat tctccaacag tgttctcatc tacatctagg 149220gcttggaaga tgaaggtgaa ggagaatatg gaaaaacaac aagaaaaaag tcaatcaatc 149280agaacttcca gccctgtgtt tgttagttca ctttatgttc aaccagtatg tctccacttt 149340tatctgcttc cttgtaagat ttcatctgaa aaagggaatt cactcttcag gtgttggaaa 149400tcaattatga gagaagcact tacagaatga ctatctgaag agctctgcag acattctcca 149460gagtaaaaca accataaccg gtgaaaatta aaaacaaaca aaataaaaaa atgttagagt 149520attggaaatt gttctaaggg ccgacagcaa atgaagaaat ccaagaaagc ctaccaattt 149580cagtgagagg agtgagagtc tatggtgttg agtctatggt gtttaagttc tgttcatttc 149640ctccctctcc tgcaatcagt tcagtgaaac agaaaccctg ctccagagat gtgcagccaa 149700aaagacaggg tgccctttcc cctcagctcc caatctaggg cagactacca gcatttccca 149760agcccatctc tggctctatg ttgcagaagt tctattccaa gaaagtttga ctgagaatct 149820gaagcttcct tcttccactc agcctctact attaaggcgt aagttctatc cccagtgcca 149880cgggccaaga atactgatgc tgaatgtcct tgctccgcta cactcatagg gcagagattc 149940catgggagga gtgaagtttc cattccagaa aaggcagaaa aacagaaatt actgcccaat 150000ccagtacccc acccataaag aaggaaagac atctgagaaa agcatgacac tgtccccaaa 150060accacctgca gagtagtggc agtagaggca gtagggagac tagaggacct gcctagaggg 150120agaagtaggc cataagaaga aaagtctcta gttctcccta aagggacaaa ctttatttgc 150180aacagaacat gcggaagttc aagcctaagg gcactgtcaa aaacaatgaa tattttggtg 150240gtaagcaatt aataggttaa tggcagctta gtgagagcaa caaactaaac cataaaccag 150300ctagacatat acaataaaaa tccaagaaag agagagccaa gaagggcctt cctgaggtta 150360aaacaaacat caaagtcttg tctgaaaaac tgctcctgca aaggaagcaa aatatatttg 150420gatcagactg tggagcaatt tatgccccta aaaagctgtc aaaaccaaaa gagcaattat 150480ctagcaattg ctggaggtta acagttgggt atgataccaa aagaggcaaa caactgaaca 150540gatcagagaa agaggcagtc aaagagagca ctgctaaatg cactgtaatt ccaatttgag 150600agaaaatgtg cccagtgctg tgactccctc aggaacaaca tcagaggcca caaattatgg 150660tggaaatagg cttcactata tagtctagcc aggcaattaa acaaataaac aattaaacaa 150720cagtaacata gtgagggggg cagaaatgag tattgcaact agctagaaca tatttttgaa 150780caaaaaaatt atgagtcaag cagagatatg gaaaagacag agccatacac cagaaaaaag 150840gtaagcaaga gaaactgcct ttgtgagtgc caagatgtca gacatagcag aaaaaaaacc 150900tcaaagtaat tattataaat atgttcaaaa actaaaagaa aatgtgctta aaaaataaaa 150960gatggtatga taatttcttg tcaattcaaa aataccaata atagattaaa aatatttttt 151020caaaagttaa ttaaccaaat ggaaattctg gggtttaaaa gtaaaataac aacaataaga 151080acattttgct agaggaaatg aacaatagat ttgtaccaga aggaaaaata atcaaataaa 151140cagggatttc atcaacacca aacctgtctt acaagaaata ctaaagggag ttcaatctga 151200aagaaagaac attaacaagc aacaggaaat catctgaagg tacaaaactc actgtacctt 151260cagatgataa taagtcctca aaaaacacag aatattataa cactgtaatt atggtgtgta 151320agctacttat atcataagta gaaagaggaa aagatgaact gattaaaaaa caattacaac 151380agcttttcaa gacagtataa taagatatat aaataacaga aagcttaaaa gcatataaat 151440aaagttaaaa tgtagaggtt tttttagttt tctttttgct tgattgtgta tttatttatg 151500caatcagtgt tgttttcatc agcataaaat aatgggttat aagatagtat ttgcaagcct 151560catagtaacc tcaaatttaa aaacatacaa tgtatacaca aaaagtgaag agcaagaaat 151620taaaacatac caccaaaaaa aatactttca ctaaaaagga gacaggaagt aagaaaagat 151680gggagagaag atcacaaaac aaccagaaaa taaataacaa aatggcaagc aagtccttat 151740ttgtcaataa taatattgaa tgtaaatgga ctatactctc cagtaaaaag atacagagtg 151800gttgaatgga ttaaaaaaaa ggacccaatg atctgttacc tacaagaaat aaacttcact 151860tataacaata cacagactaa aaataaagtc atgggaaatg atacttcacg acaatggaaa 151920ccaaaaaaga acaggagtag ctataccttt atcagactaa atagatttca agacaaaaat 151980tgttaagaaa aaccaaataa ggtcattata taatgataaa gagatcagtt aagcaagagg 152040atgtaacaat tgtaaatata tatgcactca acattggagc acctagataa ataaaacaaa 152100tattattaga gctaaagaaa gatagacccc ctcccccaaa acaataatag ctgtagattt 152160caatgctcca ctttcagcat tggacagatc ttccagacag aaaatcagca aggaaacatc 152220agatgtaatc tgaactatag accaaatggg cctaataagt atttacagaa tatttcatcc 152280aaaggttaca gaagacacat ttttctcctt agcacataca tcattctcaa gaacaggcca 152340tatattaggt catgaatcaa gtctcaaaac attcaaaaaa actgaaataa tctcaagcat 152400cttctttgac cacaatggaa taaaactaga aaccaataac aaggggaatt ttggaaacta 152460tacaaaaaca tggaaattaa acaatatgtt cctaaatgat aagtgggtca atgaagaaat 152520tatgaattga attgaaaatt ttctaaaaac aaatgacaat ggaaacataa tgcaccagaa 152580cctatgggat acaacaaaag cagtaccaag agtgatattt ccagataagg gcctacatca 152640aaaaggaaga aaaacttcaa gtgaataacc 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155160aacaaagatc caaagagaca aagaaagcca ttacataatg gtaaagggat caatacaaca 155220agaagaacaa actatcctaa atatatatgc aaccaatatg ggagcaccca gattcataaa 155280gcaagtctgt agagacgtac aaagagatgt agacttccac acaataataa tgggagactt 155340taacactcca ctgtcaatat tagacagatc aatgacacag aagggtcaca aagatatcca 155400ggacttgaat tcagctattc accaagtgga cctaatagac atctacagaa ctctacaccc 155460caaatcaaca gaatgtacat acttctcagc accacatcat acttattcta aaattgacca 155520cataattgga agtaaaacac tcctcagcaa atgtaaaaga acagaaatca caacaaactg 155580tgtctcagac tacagtgcaa acaaattaga actctggatt aataaactca ctcaaaatca 155640cacaactaca tggaaactga ataacctgct cctgaatgac tactgggtac ataatgaaat 155700gaaggcagaa ataaagatgt tctttgcaac caataagaac aaagacacaa cataccagat 155760tctccgggac acatttaaag cagtgtgtac agggaaaatt atagcactaa atgcccacaa 155820gagaaagcag gaaagaacta aaatcaacaa cctaacatcg caattaaaag aactagagaa 155880gcaagagcaa acacattcaa aagctagcag aaggcaagaa ataactaaga tcagaacaga 155940actgaaggag atagagacac aaaaagcact tcaaaaaaat caatgaatcc 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aggcaggcca acattcagat tcaggaaata 157680cagagaacgc cacaaagata ctcctcgaga agagcaacac caagacacat aattgtcaga 157740ttcaccaaag ttgaaatgaa gggaacaatg ttaagggcag ccagagagaa aggtcgggtt 157800accctcaaag ggaagcccat cagactaaca gcggatctct cggcagaaac tctacaagcc 157860agaagagagt ggtggccaat attcaacatt cttaaggaaa agaattttca aaccagaatt 157920tcatatacag ccaaactaag cttcataagt gaaggagaaa taaaatcctt tacagacaag 157980caaatgctga gaaattttgt caccaccagg cctgccctaa aagagctcct gaaggaagtg 158040ctaaacatgg aaaggaacaa ccggtaccag ccgctgcaaa atcatgccaa aatgtaaaga 158100ccatcgaggc taggaagaaa ctgcatcaac taacgagcaa aacaacctgc taacatcata 158160atgacaggat caaattcaca cataacaata ttaactttac atgtaaatgg actaaatgct 158220gcaattaaaa gacacagact ggcaaattgc ataaagattc aagacccatc agtgtgctgt 158280attcaggaaa cccatctcac gggcagagac acacataggc tcaaaataaa gggatggagg 158340aagacctacc aagcaaatgg aaaacaaaaa aaaggcaggg gttgcaatcc tagtctctga 158400taaaacagac tttaaaccaa cacagatcaa aagagacaaa gaaggccatt acttaatggt 158460aaagggatca attcaacaag aagagctaac aatcctaaat atatatgcac ccaatacagg 158520agcacccaga ttcataaagc aagtcctgag tgacctacaa agagacttag actaccacac 158580attaataatg ggaaacttta acaccccact gtcaacatta gacagatcaa caagacagaa 158640agtcaacaag gatacccagg aattgaactc agctctgcac caagcagacc taatagacat 158700ctacagaact ctccacccca aatcaacaga atatacattt ttttcagcac cacaccatac 158760ctatttcaaa attgaccaca tacttggaag taaagctctc ctcagcaaat gtaaaagaac 158820agaaattata acaaactgtc tctctaacca cagtgcaatc aaactacaac tcaggatgaa 158880gaaactcact caaaactgct caactacatg gaaactgaac aacctgctcc tgaatgacta 158940ctgggtacac aacgaaatga aggcagaaat aaagatgttc tttgaaacca atgagaacaa 159000agacacaaca taccagaaat tctgggatgc attcaaagca gtgtgtagag ggaaatttgt 159060agcactaaat gcccacaaga gaaagcagga aagatccaaa attgacaccc taacatcaca 159120attaaaagaa ctagaaaagc aagagcaaac acattcaaaa gctagcagaa ggcaagaaat 159180aactaaaatc agagcagaac tgaaggaaac agagacaaaa aaaacccttc aaaaattaat 159240gaatccagga gctggttttt tgaaaggatc aacaaaattg atagaccgct agcaagaata 159300ataaagaaaa 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160140tgccctctct caccactcct attcaacata gtgctggaag atctgtccag ggcaatcagg 160200caggagaagg aaataaaggg tattcaatta ggaaaagagg aagtcaaatt gtccctgttt 160260gcagatgaca tgattttata tctagaaaac cccattgtct cagcccaaaa cctccttaag 160320ctgataagca acttcagcaa agtctcagga tacaaaatca atgtacaaaa atcacaagca 160380ttcttataca ccaacaacag acaaacagag agccaaatca tgagtgaact cccattcaca 160440attgcttcaa agagaataaa atacctagga atccacctta caagggatgt gaagcacctc 160500ttcaaggaga actacaaacc actgctcaac gaaataaaag aggatacaaa caaatggaag 160560aacattccat gctcatgggt aggaagaatc aatattgtga aaatggccat actgcccaag 160620gtaatttaca gattcaatgc catccccatc aagctaccaa ttactttctt cacataattg 160680gaaaaaacta ctttaaagtt catatggaac caaaaaagag cccgcatcac caagtcaatc 160740ctgagccaaa agaacaaggc tggaggcatc acactacctg acttcaagct atactacaag 160800gctacagtaa ccaaaacagc ttggtactgt taccaaaaca gagatataga tcaatggaac 160860agaacagagc cctcagaaat aatgctgcat atcgacaact gtctgatctt tgacaaacct 160920gagaaaaaca agaaatgggg aaaggattcc ctatttaata aatggtgctg ggaaaactgg 160980ctagccatat gtagaaagat gaaactggat cccttcctta caccttatac aaaaattaat 161040tcaagatgga ttaaagactt aaatgtcaga cctaaaacca taaaaaccct agaagaaaac 161100ctaggcatta ccattcagga cataggcatg ggcaaggact tcatgtctaa aacaccaaaa 161160gcaatggcaa caaaagccaa aattgacaaa tgggatctaa ttaaactaaa gagcttctgc 161220acagcaaaag aaactaccat cagagtgaat aggcaaccta cagaatggga gaaaattttt 161280gcaatctact catctgacaa agggctaata ttcagaatct acaaagaact taaacaaatg 161340ttcaagaaaa aaacaacccc atcaaaaagt gggcaaaata catgaaaaga cacttctcaa 161400aagaagacat ttatgcagcc aacagacaca tgaaaaaatg ctcatcatca ctggccatca 161460gagaaatgca aatcaaaacc acaatgagat accatctcac accagttaga atgacgatca 161520ttaaagtcag gaaacaacag atgctggaga ggacatggag aaataggaat gcttttacac 161580tgttggtggg ggtgtaaatt agttcaatca ttgttgaaga cagtgtggca attcctcaag 161640gatctagaac tagaaatacc atttgaccca gcaatcccat tactgggtat ataccgaaaa 161700gattacaaat ggtgcaacta taaagacaca tgcacacgaa tgtttattgt ggcactattc 161760acaatagcaa agacttctaa caaacccaga tgtccatcaa tgatagactg gattaagaaa 161820atgtggcaca tatacaccat ggaatactat gcagccataa aaaaggatga gttcatgtcc 161880ttttcaggga catggatgaa gctggaaacc atcattctca gcaaactatg acaaggacag 161940aaaaccaaac accgcatgtt ctcactcata gatgggaact gaacaatgag aacacttgga 162000cacagggcag ggaacatcac acaccagggc ctgttggggg gtggtggact gggggagaga 162060tagcattagg agaaatacct aatgtaaatg atgagttgat ggatgcagca aaccaacagg 162120gcacatctat acctatgtaa gaaacctgca ctttgtgcac aggtacccta gaacttgaag 162180tataataaaa atgaaaaaaa gaaaatgtta ctagcaaatt gtagatgggt atacctaata 162240taactctgca tgtgcactat gtgtctggat acacatggaa agtattcagg catacacacc 162300aaattgttaa aataaatccc cttccaacat taggagtggc tggggagcag atggctttca 162360cttccgcaca ttccatggtg ccagggtaat accacatcac aatagtaaat agcaaaaata 162420acaaccttaa gtttagattt tcttatctaa attttaattc tgtgaagaag tttccatttc 162480cctttttcac acaggatagt agaatgcagt ttagaaagag tgagatcact tgttagactg 162540tacaattttt aagcatcagc gaagtatacc attaaacttc tctatcaatc cattctctaa 162600acttcccaac ccaaaaaaaa agaaacacat aaaattgaaa tgaagaaaac aatacaaaag 162660atcaacaaaa atgaaaggtt ggctttttaa agagataaac aaaattgaca aatgtttagc 162720cagactaaga aaaagagaag gcccaaataa ataaaatcag agatgaaaaa gcagacatta 162780taactgatac tgcggaaatt caaaggatca ttaatggcta ctatgagcaa ctgtatgcca 162840aacattggaa aatattgaag aaatagataa attcgtagac acacgcaacc taacaagatt 162900caaccatgaa gaaattcaaa atctgaacag accaaaaaca agtaacaaga tcaaagccat 162960aattaaaatt ttcccagcaa agaaaagcct gagacccaat ggcttcactg ctgaattcta 163020tcaaacattt aaagaactaa taccaatcct actcaaacta ttccaaaaag tagaagagga 163080gggaatattt ccaaacttat tctatgaggc cattactgct cctatatcaa aaccaaggac 163140acatcaaaaa aagaaaacta taggccaata tctcacatga atattgatga atcctcaaaa 163200aatgctagca aactgaattc aacatcacat taaaaatcat tcattatgac caaatgggat 163260ttatcccagg gatgcaaata tggttcaaca tatacacatc agtcagtgta acatatcata 163320tcaacagaat gaagaagaaa aaccatatgg tcatgttaat tgatgctgaa aaagtatttg 163380agagaattaa acatctcttc atgacaaaaa ccctcaaaaa cactggagac agaaggaaca 163440tacctcaaca caataaacag acatatatga cagactcaca gctagaatca tactaaatgg 163500ggagaaactg aaagtcattc ctctaagatc tggaacaaga caaggatgct cattttcacc 163560agtgttagtg gaacatagta ctgaaagtcc tagctagaac agactagaga cagaaataag 163620gggcatccaa actgggaaga gagaagtcaa attacctttg ttcgcagatg gtatgatctt 163680ctgtttggaa aaacctagac tccataaaaa aatgattaga actgataaat tcagtaaagt 163740ttcaggatac aaaattaaca tacaaaaatc agtagcattt ctatatgtca atagcaaaca 163800atctgaaaat gaaatcaagg aagtaatccc atttcccatt tgcaatagct ataaataaaa 163860ttaatataat ctcatctctt caataagtgg tgttgggaaa actgcatatc cacatacaaa 163920agaataaaat tagacccttt taatatacaa aactggaccc aagttaatat acaaaaatta 163980acttgaaatg aattaaagac ttaaatgtaa gatctgaaac caaaaactcc tagagagaaa 164040cataggggaa aagctccttg acgttggcct tggcaataat tttttgggat attacaccaa 164100aagcacagac ttttgaaaaa ataaacaagt aggactacat caaactaaaa agcttctgca 164160tggcaaaaga aagtcaacaa catgaaaagg caacctacag aatggaggga aatatttgca 164220aaccatatac ctgatgagaa gttaatatca aaatatagaa aatatataag gaactcacat 164280atctcaatac caaaaaaata ataacctgtt ttataatggg caaaggacct gaatagacat 164340tttttcaaag aagacacaca gatggccaac gagtgcagga aaaagcgttc aacatcacta 164400atcatcaggg aaatgcaaat caaaaccaca atgagatatc actccacacc tgttaggaaa 164460gctattatga aaaacacaag agacaatgaa tattggcaag ggcatggaga aaaaagaacc 164520cttgtacact gttggtagaa atgtaaattg caacagcctt tatggaaaat agaatggagg 164580ttcctcaaaa aataaaaata gaactaccat acaatctagc aattccactt atgagtgtac 164640atccaaagga atcaaatcac tatgtcaaag agatatctgt acttccttgt ttattgcagc 164700tttactcaca gtagccaaga taaggaaaaa atctaaatgt ccatcaacgg ataaagaaaa 164760tggggggagg ggtgtgcatg tatacataca caatggacta tttttcagcc ataacaaaga 164820aggaaaccct gccatttgtg acgatatgaa tgaacccaga ggacattatg ttaagtgaaa 164880taagccagac acagaaatac aaatattgta tgatctcatt tatatgcgaa tctaaaaatt 164940tcaaacttga agacgaataa gtagaacagt atttatcagg agctagggga tgtggggaag 165000aagcaaaata ttgggcaaag agtataaact ttcagttatg agatgaaggc caggtgcgat 165060ggctcatgtt ggtaatccca atactttgga aggctgaggc agagggattg cttgagacca 165120gcctaggcaa gaaagtgaga cctcatctct acaaaaaata aaaataaaag aatcagctgg 165180gagtggtggc atacacctgt agtcccagct agtcaggagg ctgagggggg aggatcattt 165240gaaattggaa agtcaaggct gcagtcagcc aagatagtgc cactgcattg cagcctgggt 165300gacagagcga aaccctgtct caaaaaaaaa aaaaaaaaaa agatgaataa gttctgggga 165360tcaaatgtac agcatggtga ctatagttta taactgcgtt attacttgaa attggataag 165420agcagatttt aagcatcccc agcacccccc caacatacac acacacaaat ggtaactata 165480ggtggtgata gatatgttaa tttgactgtg acaatcagca ttcaatatat acatatatca 165540aatcatcaca ttttacccct tgaaatagaa actttgattt gtcaatcaaa tattttaaaa 165600cgaaaataat cataatatta atatagcata caggaaaaaa tttcgtatct cgactgatac 165660agaaaatttc atgataaaaa cactttaaaa caaaagaaat aaaagggaac tccctcaacc 165720tgataaatgg catctgtgga aacccccagc tagcatcaaa cttaatacag aaaggctggg 165780tgttcacctc ttgaaccagg aacaagacaa agatgcctgc ttttgccact tccatttgac 165840cttgtactga ggttctggct agggcaatta tccctgaaaa agaaataaaa ggcttccaaa 165900taaaagaaga agtaaaacta tctctactcg ctcatgacat gatcttgcat atagaaaatg 165960tgcacatgta cacacacaca aaccattaga actaataaac aagttcagca agtttgcaga 166020atataaaatg aatgtacaaa aatcaagtgt ttttctatat actagcaatt aacaatctca 166080aaatgaatta aaattccatt tacaatagta tcaaacataa attatttaga aataaaaagt 166140gcactgaaaa ctacaaaata ttttgaaata aatcagaaaa gatttaagta aatggatcac 166200ttgaacctgg gaagcagagg ttgcagtgtg ccgagattgt accactgcac tttagcctgg 166260gcaacagagg gagactccaa agagtcgaaa agaaaagaaa agatttaaat aagctgaaac 166320atattctatg gatcagaaga cttaatattg ttaaagtgac aatattcccc aaattgatct 166380acagcttcaa ctcaacccct atcaaaatcc tagcttgctt tttggctgaa attgacaagc 166440tgattctata atttatatgg aatctcaaag gatccagaat aaccaaaaca atattgaaaa 166500ataaagaaca gcgttggtgg attaacattt tccaatttca aaacttacta tagcactgcg 166560gtaatcaagc agtgtggcac tgtatagcat gtacattaca gatcagtgga ctagaatcaa 166620tgtccagaaa taaaccgtta tgtttataat gaattacttt ttaataaggt gtcaagacaa 166680cgcaatggga aaagaataat gaattcaaca aatgatgcat ggacaaccgg acatgcacat 166740gcaacacaat gaatttgaat tcttctatcg ctccatgcat aaaaactaac tcaaaatggg 166800tcacggatgt aaatgaaaag ctaaaactat aataatccta gaggaaaacc taggagtaaa 166860tctttaagat gttattgtag gcagtggttt ctcagatagg accccaaaat cacaagcgac 166920aaaagaaatt ggacttaaag ttaaatactt ttgtgcttca aacatcatca agaaagtgaa 166980aacacaaccc gcagaagcaa taaaaatgtc tgtaagtcat gtatccgatt agagacttct 167040atccaggata tataaataat gcaattcaat gataaaaaag ataaatagcc cagttttcca 167100aagagtcaag catctgaata tacatctctc caaaaatata cagatatcca acaagcatgt 167160gaaaagatgt tcaaagccat ttgccaggtg cacaaaccca agacagtatg aggagatgct 167220acagggactc tgctgcttca cagacatgaa gcgttggtga gaatgtaggc agccgccttt 167280ggggacttca catccccgcc gccccacgca cggtgagcta gtgtttaaac ttagccgaga 167340tcaatacacg cgactgtgtg cccgtcagac cctgcgctgc cggcggggct gggagaggcg 167400ggcgccagga gtgggcggga acctgggggt caggccccag ccgcgggaag ccgcccagga 167460gcgcgcgaaa ccttctccac acccttccag gcatttgccc gccgcgattc agagagccga 167520cccgtgaccc ctggcctccc ctagacagcc ccgcatgtcc agatgtgccg tcccgcctgc 167580ctcccgcgac cactggccat ctctgggcct gggcgcggtc tcggcgcccg cctgcccccg 167640ccaggagccg caggtccagc cagtgaagaa gcccgcgctg aaggagcctc tgtgctccag 167700aatccatcct cagtatcagc gctggggtgg cctcctccag gaagcccttc tgattctctc 167760atgggtcgct cttcctctgc agactcccgg agcacccctg ctccaagtac cgcaagtggc 167820actgagaact tggggagagc agaggctgtg cctagatttg tagggagtcc ccgcagctcc 167880accccagggc ctacaggagc ctggccttgg gcgaagccga ggcaggcagg cagggcaaag 167940ggtggaagca attcaggaga gaacgagtga acgaatggat gaggggtggc agccgaggtt 168000gccccagtcc cctggctgca ggaacagaca cctcgctgag gagagaccca ggagcgaggc 168060ccctgccccg cccgaggcga ggtcccgccc agtcggcgcc gcgtgaagag tgggagagaa 168120tactgcgggg gcgggggcgg gggcgggggc gggggcgggg gccgccggga gcctggagcc 168180agaccgggcg gggccggcac cgggccaggg acagtggggg aggaggctgc gggctgagcg 168240accctgaccc cccccagtcc gcgctggttc cgg 168273 3 2497 DNA Homo sapiens 3gggcgccgag gctccccgcc gctcgctgct ccccggcccg cgccatgccc tcctacacgg 60tcaccgtggc cactggcagc cagtggttcg ccggcactga cgactacatc tacctcagcc 120tcgtgggctc ggcgggctgc agcgagaagc acctgctgga caagcccttc tacaacgact 180tcgagcgtgg cgcggtggat tcatacgacg tgactgtgga cgaggaactg ggcgagatcc 240agctggtcag aatcgagaag cgcaagtact ggctgaatga cgactggtac ctgaagtaca 300tcacgctgaa gacgccccac ggggactaca tcgagttccc ctgctaccgc tggatcaccg 360gcgatgtcga ggttgtcctg agggatggac gcgcaaagtt ggcccgagat gaccaaattc 420acattctcaa gcaacaccga cgtaaagaac tggaaacacg gcaaaaacaa tatcgatgga 480tggagtggaa ccctggcttc cccttgagca tcgatgccaa atgccacaag gatttacccc 540gtgatatcca gtttgatagt gaaaaaggag tggactttgt tctgaattac tccaaagcga 600tggagaacct gttcatcaac cgcttcatgc acatgttcca gtcttcttgg aatgacttcg 660ccgactttga gaaaatcttt gtcaagatca gcaacactat ttctgagcgg gtcatgaatc 720actggcagga agacctgatg tttggctacc agttcctgaa tggctgcaac cctgtgttga 780tccggcgctg cacagagctg cccgagaagc tcccggtgac cacggagatg gtagagtgca 840gcctggagcg gcagctcagc ttggagcagg aggtccagca agggaacatt ttcatcgtgg 900actttgagct gctggatggc atcgatgcca acaaaacaga cccctgcaca ctccagttcc 960tggccgctcc catctgcttg ctgtataaga acctggccaa caagattgtc cccattgcca 1020tccagctcaa ccaaatcccg ggagatgaga accctatttt cctcccttcg gatgcaaaat 1080acgactggct tttggccaaa atctgggtgc gttccagtga cttccacgtc caccagacca 1140tcacccacct tctgcgaaca catctggtgt ctgaggtttt tggcattgca atgtaccgcc 1200agctgcctgc tgtgcacccc attttcaagc tgctggtggc acacgtgaga ttcaccattg 1260caatcaacac caaggcccgt gagcagctca tctgcgagtg tggcctcttt gacaaggcca 1320acgccacagg gggcggtggg cacgtgcaga tggtgcagag ggccatgaag gacctgacct 1380atgcctccct gtgctttccc gaggccatca aggcccgggg catggagagc aaagaagaca 1440tcccctacta cttctaccgg gacgacgggc tcctggtgtg ggaagccatc aggacgttca 1500cggccgaggt ggtagacatc tactacgagg gcgaccaggt ggtggaggag gacccggagc 1560tgcaggactt cgtgaacgat gtctacgtgt acggcatgcg gggccgcaag tcctcaggct 1620tccccaagtc ggtcaagagc cgggagcagc tgtcggagta cctgaccgtg gtgatcttca 1680ccgcctccgc ccagcacgcc gcggtcaact tcggccagta cgactggtgc tcctggatcc 1740ccaatgcgcc cccaaccatg cgagccccgc caccgactgc caagggcgtg gtgaccattg 1800agcagatcgt ggacacgctg cccgaccgcg gccgctcctg ctggcatctg ggtgcagtgt 1860gggcgctgag ccagttccag gaaaacgagc tgttcctggg catgtaccca gaagagcatt 1920ttatcgagaa gcctgtgaag gaagccatgg cccgattccg caagaacctc gaggccattg 1980tcagcgtgat tgctgagcgc aacaagaaga agcagctgcc atattactac ttgtccccag 2040accggattcc gaacagtgtg gccatctgag cacactgcca gtctcactgt gggaaggcca 2100gctgccccag ccagatggac tccagcctgc ctggcaggct gtctggccag gcctcttggc 2160agtcacatct cttcctccga ggccagtacc tttccattta ttctttgatc ttcagggaac 2220tgcatagatt gtatcaaagt gtaaacacca tagggaccca ttctacacag agcaggactg 2280cacaggcgtc ctgtccacac ccagctcagc atttccacac caagcagcaa cagcaaatca 2340cgaccactga tagatgtcta ttcttgttgg agacatggga tgattatttt ctgttctatt 2400tgtgcttagt ccaattcctt gcacatagta ggtacccaat tcaattacta ttgaatgaat 2460taagaattgg ttgccataaa aataaatcag ttcattt 2497 4 21 DNA Homo sapiens 4aggagcgcgc aaaaccttct c 21 5 20 DNA Homo sapiens 5tggacttaaa tacttttgtg 20 6 21 DNA Homo sapiens 6tcatgtatcc aattagagac t 21 7 21 DNA Homo sapiens 7acttactata acactgcggt a 21 8 21 DNA Homo sapiens 8ttacagatca atggactaga a 21 9 18 DNA Homo sapiens 9gggccaggga ccagtggt 18 10 18 DNA Homo sapiens 10aaccgggtcc cggacgca 18 11 19 DNA Homo sapiens 11aggctcagga gaccacgca 19 12 16 DNA Homo sapiens 12tcccgcccct gcacag 16 13 24 DNA Homo sapiens 13cattgggcat tgttattgtt cttc 24 14 25 DNA Homo sapiens 14agttgtaggt aaggtgaagt ttggg 25 15 19 DNA Homo sapiens 15tcgtctgaca gtgtgggcg 19 16 22 DNA Homo sapiens 16ccatgaggaa aggagtgagg gt 22 17 19 DNA Homo sapiens 17tggtgtgaag gggctctgc 19 18 25 DNA Homo sapiens 18aagtatcaga caggagagca gcatc 25 19 21 DNA Homo sapiens 19gcctcgcttt tctcctggta g 21 20 19 DNA Homo sapiens 20ccacttcccc agcccatca 19 21 22 DNA Homo sapiens 21gatttttggt ccgtctgctg ag 22 22 18 DNA Homo sapiens 22gaaaggtgtg ccccccag 18 23 22 DNA Homo sapiens 23tttcctttcc cccaatgtat ca 22 24 18 DNA Homo sapiens 24gggcggtagc tggctgta 18 25 18 DNA Homo sapiens 25gagctgcggg tccctgag 18 26 20 DNA Homo sapiens 26agatagggag tgaaggcggc 20 27 18 DNA Homo sapiens 27agccacccgc tcagggca 18 28 17 DNA Homo sapiens 28aggcaggggt cccggct 17 29 17 DNA Homo sapiens 29tccgcagacc tggctgg 17 30 16 DNA Homo sapiens 30gaggggtggc ggaggg 16 31 18 DNA Homo sapiens 31ccggtggttc caccctag 18 32 19 DNA Homo sapiens 32gggaggaggc agcggcctt 19 33 17 DNA Homo sapiens 33tgctggcggt cgtctcc 17 34 16 DNA Homo sapiens 34cgccctgccc agcttc 16 35 28 DNA Homo sapiens 35tgggatgatt attttctgtt ctatttgt 28 36 25 DNA Homo sapiens 36gagtagacac tgcttgaggg aaaaa 25 37 18 DNA Homo sapiens 37gcgtcctgtc cacaccca 18 38 26 DNA Homo sapiens 38tgaactgatt tatttttatg gcaacc 26 39 18 DNA Homo sapiens 39gcccgattcc gcaagaac 18 40 24 DNA Homo sapiens 40gaggaagaga tgtgactgcc aaga 24 41 25 DNA Homo sapiens 41cagtttacac gggtagtgga ttgac 25 42 23 DNA Homo sapiens 42gaagagatgt gactgccaag agg 23 43 23 DNA Homo sapiens 43cctcttggca gtcacatctc ttc 23 44 28 DNA Homo sapiens 44acaaatagaa cagaaaataa tcatccca 28 45 23 DNA Homo sapiens 45ctaactcaaa atgggtcacg gat 23 46 20 DNA Homo sapiens 46attgcttctg cgggttgtgt 20 47 18 DNA Homo sapiens 47gagagccgac ccgtgacc 18 48 24 DNA Homo sapiens 48gctgatactg aggatggatt ctgg 24 49 21 DNA Homo sapiens 49tgaaaacaca acccgcagaa g 21 50 19 DNA Homo sapiens 50tgcacctggc aaatggctt 19 51 20 DNA Homo sapiens 51aaagaacagc gttggtggat 20 52 22 DNA Homo sapiens 52caaattcatt gtgttgcatg tg 22 53 24 DNA Homo sapiens 53aacttagccg agatcaatac acgc 24 54 19 DNA Homo sapiens 54gcaaatgcct ggaagggtg 19 55 24 DNA Homo sapiens 55gcacaaaccc aagacagtat gagg 24 56 18 DNA Homo sapiens 56cggcggggat gtgaagtc 18 57 20 DNA Homo sapiens 57tggcactgag aacttgggga 20 58 18 DNA Homo sapiens 58actggggcaa cctcggct 18 59 24 DNA Homo sapiens 59gctccagaat ccatcctcag tatc 24 60 21 DNA Homo sapiens 60gcctctgctc tccccaagtt c 21 61 6 DNA Homo sapiens 61ggcggg 6 62 6 DNA Homo sapiens 62gttaaa 6 63 168174 DNA Homo sapiens misc_feature (1)...(168174) n = A,T,C or G 63ttgcacgctn ctcagccccg gctgtactat tggtctacac gtgccatcgt ggtttgctgc 60acccatcaac ccatcatcta cattaggtat ttctcctaat gctctctttc cccttgcccc 120ccaccctcta acacgccctg ttgtgtgatg ttgacctccc tgtgtacatg tgttgtcatt 180gtacaactcc tgcttatgag tgacaacatc tgtctgattt tctgtcttgt gttagtttgc 240tgagaatgat ggttcccatc ttcatccttg tacctgcaaa ggacatgaac tcatccttta 300atatggctgc ttagaatccc acagtataga tgtgcctcat tttctttatc cagactatca 360ttgatgggca tttgggttgg atccaagact ttgctattgt gaacagtgat tcaataaaca 420tacgagtgca tgtgtcttta tggatgaatg attgataatc ctttgggcat atacccacta 480atgggattgc tggacctaat ggtttgcctg gatcctgatc cttgaggaat caccacactc 540tcttacagtg taaaagtgtc tctatatata ctcatcccct ccagcatctg ttatttactg 600actctttaat catcaacctt cttactggag agagaaggtt taattaccac taatgacgag 660cctttaatca aattgttgtt ggacccttcc caacttactt gcgaaggggt catttgtaaa 720cttacgccac cttttgatgg ggcagttttt ctcgtttatc cgcaaagatc ctgggggacc 780ccttttttta actcttttga agaccgttat atctgaacaa tttctcccat accgagaggt 840ggcgggttac aacccttgaa agtttctttt tttttttggg gccccttagt ttcacaaaaa 900tcccttcgga ccaatttgcc ttttgttgcc aaggaatttt tccccctaag tcactaggaa 960tttttcccag ggcacacagg tgtagtggcc ttccccagaa tttttctcct gggtatccaa 1020tgttataaga aaaaaaattg gggaattttt ttaaacctcc aataaaattt tttatagaga 1080taaggccggt ttcatttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc 1200ggtcttcagc ccgtggtgaa gactgattaa cagtcataag aaggtaaagc tctaagtgac 1260ctgtaagaaa catacagtct cacacaatta ggcaaggagt ccaacttgga agaaagagtg 1320gcatagattc aaaccctgat gccacaactt atgttaaccc tcctgaggct caacttctca 1380cacatgacag gagaatctca tcatgtacct caattaattg atatacagat tatggtaaac 1440attcaatgaa aaagtatgta aagtttctat cattctacta cagttggtat tcaacaaatg 1500ccactttatt cactttctgt taagcctgaa agaagtactg aatgacatca acgagtgcaa 1560ttcaacaaat ataatttgag cactgattac tcaataagca ttatgaggaa ctaaaatatt 1620atcagcaaat gatggctgac atagaggaaa tgtgacagag taggaagatc cctgtactcc 1680ctgtatcaaa ggactgagat tcttccactc aggaccatgt gtgaccccaa cagaaagtag 1740aaatttacag aaagatacct ggcaaaacac ccaaatagtt tgaagttaaa aaatatacct 1800ctgaataaca ccccatggaa atttttaaag attataatgt gaattaaaat gaaaacatat 1860aaccataaca aaaattgtag gatacaggaa aactggtgct catatagcat taactgttta 1920cattacattt tagagaatca acttaattca attatctaag tttctatttt agaaaactat 1980gtaaaaaaag gagagcatat ttaaaaagta agcagaagaa caaaagataa gcatgacaga 2040caatgaaatt gaaaatggaa aaatcataga gaagaatcaa atgaatcaaa agtagtttct 2100tggaaaagat caataaataa aattggttga tataatttac tgatattagg agtgggaaag 2160ataatcactg atcatagatc catgatgaac aagcttattt caataaatta ataaactcag 2220caacttcaac aaaatggaca gattacttgg aagacaccaa ttaccaaagc tcaaaaaaag 2280agaatctaaa tagtcctata tttatgaaaa aaaaaaacaa aactgaattt gtatttaaaa 2340actttgccac aaagaaaatt ttaggttcag atgggtttcc taataaattt ttcccattta 2400gtaagaataa tatcaattct acacaaactc attcaaaaaa aaaggagaga gaatacttta 2460caacacgttc tgcaaggtca gtattaccct gataccaaaa caaaggatat tacataaaaa 2520cgacaccaat atccttcatg aacatagatt cttcaccacg gggctggaag gatccgnnnn 2580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatag cacgcccttg agcccctggt 2700gaatataaga gattaggcta cagcgtgttc tactccaact ttatttgctt tctgagcatc 2760taattccact ttgcaaaatg gacatttttt ttccctcttg agagtcagga aattaccagg 2820tgtgccagga taatccttag tatcaactgt aaaatatcag tataaaataa tgagtaagca 2880tatttcagtg ggtgtttata tgcttttggt tgcatagcat gagccaaagg cagataaaaa 2940atgtgggctc cagatagtga ctcaatctcc agaaatcata atgtcagtta cacaatgata 3000tttctatatt ggcatcacaa aataatccac ttgtcatagg ataattgctc tagatgaggg 3060catggagctg tgctgaggaa tctgccattt ttctttatcc agactctgtc tcataccgac 3120cacaggcccc aatcattgtc tatcaaaaaa catgcttgtg tctcttttgg cttactatga 3180aaggaagcaa gatcttttcc tcaagcattt aagtgttgac tgtgggctac ccagtgtgct 3240agacactgng gatcaaattc tgtgaggtta aaaatacccc ataactcact ggatagaaat 3300tcaaaatgct cgctatgttg attctatgat caacttccaa ggggaccttt ntacctccta 3360tcttcatcag caagcaacta cattgatttt gtagcccagg atctattccc acgtcaaccg 3420tgggtttcaa atgtttgctc tagctcaatg gcctcttaag ccactaaccc aatatgttgc 3480tttttaccag taaaaaagtg catctgggga ataagttggt gtatatttgt ggttcagcca 3540gcccatagat cgtccagagg accccaggct taggaggaat cctcagattt gagagccact 3600gtcctgtatg atgcagatag cacagctctt cctgaaaata ggaattcaaa tgaggaaaaa 3660tgaaaactct cctaattatt ttcttctgcc tctttcattc caagctcatc taattatcat 3720gctctattgt gaagaatcaa agaaagaact cataaatata ccttttgtgc ctgtattttg 3780aatgtacgtt gtgcatgtaa gcctttgtgg ttaagcaaat gtcaagaaaa tccctagtcc 3840cattgcttac agtcaatatg gggcaattca agcaaagtat atgcctctaa tatggtttga 3900aaattctgaa agttcagaaa aaatgttgct acttcagcca tctgatgtta ctggatattg 3960agaatgagac ctcctctccc tctgctttcc tcatttccat ctcctccttc ctctttctcc 4020tctctccctc tcctttattc tccttctccc tcctctctct ccatgcttac tcccttcctc 4080ttctccctct ttcttcttta ttctccttct ccctcctctc tctctctccc ctttttcctc 4140ccttcctctc ctccctccct ctcctttatt ctccttctcc ctcctctctc tctctccccc 4200ttttcctata ttcccagatt aggccatttt caaaactggt taaacattcc ccaaaagcat 4260ttatgacaga gaattgacta ggatctcacc tgaatcacag agcttgccca ttgaacacag 4320gcaaagatca agccattttc tcttgatgtg atgcttcagc attttatgca tttgaaacaa 4380attttatgta aaaaaaagct tagccctttg gtgacgggga ttccttttct ggatgatctg 4440ccaaagaatc aagacccaac gtccagctta tcaaattttc tctggacctt tannnnnnnn 4500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgtggtg aagacttcca cgtgaacttt 4620aaagtagttt tttccaattc tgtgaagaat gtcattggta gcttgatggg gatggcactg 4680aagattttct tcatttttat gactgcatag cattccatgg agtatatgta ccacattttc 4740tttatccaat tcactgttgg tgggcaccta ggtcaattcc atgactttgc tgttgtgaat 4800agtactgcaa tgaacatgtg agtgcatgtg tctttttgga ggaataattt gttctctttt 4860ggatatatac ccagtaatgg ggttgctggg ccaaatggta gttctgtttt aagttctttg 4920agaaatctcc aaactgcttt ccacagaggc tgaactaatt gacattccca ctgccatgca 4980tgagcattcc cttttctcct cagctccacc aacatctgtt gctttctgac tttttgataa 5040tagccattct gatgtgtgag atggtagccc attggggttt cttttttttt ttttaagaca 5100gagtctcact ctgtcaccta ggctgtggca caatgttggc tcgctgcaac ctctgcctcc 5160tgggttcaag tgattctcct acctcagcct actgagaagc tagggttaca ggcacctgcc 5220accacaccca gctaattttg gtatttttgg tagagacagg gtttagccat attggcaagg 5280ctggtctcta actcctgacc tcaggtgacc cacctgcctc ggcttcccaa agtgctggga 5340ttgcatgcgt tagctaccat gggtggtttt tgacttgcat ttctctgatg attagtaatg 5400atgatccttt tttcatacat ttgttggcca cttgtatgtc ttcttttgag aagtgtctgt 5460tcatatcttt tgcccatttt tcaatggggt tgttttttcc ttgtccagtt gtttaagtta 5520tttatagatt ctggatatta gtcctttgtt gggtgcataa tttgcaaata ttttatctca 5580ttctgtaggt tatctattta atctgttgat agtgtctttt cttgtacaga agttgttcag 5640tttaattagg tctcacttgt caatttctgt ttttgttgta attgcttttg aggacttaat 5700cataaattct ttcccaaggc ccatgtccag aatggtgttt ctgagatttt cttctaggat 5760tcttttactt caaggtctta tatttaaatc tttaatccat ctggagttaa tttttgtatg 5820tggcaaaagt taggtgtctt gttttatttt tctgcatatg gctagtcagc tatcccagga 5880ccattcattc actagggagt cctttcccca ttgtttactt ttggcaactt tgtcaaagat 5940tagatggctg taagtgtgca gatttatttc tcagctctct attctgttca actggtgtag 6000gtatctgttt tcgtactagc accatgctgt tttggttact gtagtcttat tagtatagtt 6060tgaagccagg taatgtgatg cctctggctt tgttcttttt gcttaggatt gctttttggg 6120ctctctgttg ttgttgttcc atatgaattt taggacactt ttttccaatt ctataaaaaa 6180atgacattgg tagtttggta ataatagcat tgaatctgta gatagattgc tttgggcagt 6240atggccattt taatgatatt gattcttcca atccctgagc atggaatgtt tttccattta 6300tttgtgtcct ctctgatttc ttttagcagc gttttatagt tctccttgta gagatctttc 6360acctccttgg ttagctgttt tcctaggtat tttaattttt tgggcctttg taagtgggat 6420tgcaagcttg aattggttct tagcctgaat gttattggtt tatagaaata ctactaattt 6480ttgccatcca ttttgtatcc tgaaacgtcc ctgagtcatt tgttaattcc cagaggcttt 6540gtggagtctt taggttttct atttttagaa tctattcttc ccaaagagaa atactttccc 6600ctctcttttc ctaatcggac ccctttattt attcctttgc ccaaggcttg gaaagccctt 6660ccatactttt ttaaaaagaa cgtggaaaaa ggcccctttc ttattctcgt ttaaaggaac 6720cttccatttt cgctgtcaaa tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6840nnntttgttg ggaatatccg gtgcttattc ctcttcagga gttttattct tccaaaaatg 6900cagcttttca ttcctcctcg tttttctttc tgtgccaaat gcctccagcc tttccatgtt 6960ggtgcggtaa gctagacatt tttagtttgt agtgattgtt ttcgtttgtg tttgtatgtt 7020tttgtgccca tagtcacttc tgtaatgttc gtttctgagt ttcctttggg gggattaacc 7080tgccactgaa gtgtcagctt ccctgtcatg ggactgtcct tggaatgaca ggttttcctt 7140ccaccatagg atctgccact gtgaaggaga tgatgagagc cccctgatca ccccctgcca 7200ctgcacagga agcctccact tcgtgcacca ggcctgcctg cagcagtgga tcaagagctc 7260cgacacgcgc tgctgcgagc tctgcaagta tgagttcatc atggagacca agctgaagcc 7320actgagaaaa gtacgtggga ggaagggatg cttcaccctt cctgaaacca gtgaccccca 7380gaaagctggt gtctcagatc atgaggtcat ggcttgcctg cgttcatttc cttaaaaagc 7440tggggctggt gcttacattt gcattgtaga gattgttgtt tctggtgctg attttcaagt 7500ccctcttctc aggaagaagc tctttctgac cattcataat ccttcttttc ccttcaaagt 7560aagactgctt gcttgtcggt gtcagtcctc tggcctttga ctacagactg tttgtgacat 7620atcttagcac ctaattttat acatattaac tgttgacgtc ctgcttctct gattaggact 7680gtctgttgga gattagtatc tccccagcac ccagcacagt gtagcataca gtaaatttat 7740ttgtatacac ccatgataac tatttgctgg atgggagtgt gagtaggtgg atagggaaaa 7800gaaaggaagc acaaacgttg cgtgatgtgc aactgtcttc agaaaggacc cacaggcatt 7860gcatgacacg cagctgtctg gaggcccatg tcactgctcc ctctgagagg agctttgtcc 7920gccacactgg cttcctgttt aaattttgac tgtttcatat atctctctag atgcccagca 7980caaaaggttt tgtctggaag aataaaaaac tgttcctatc tcagaacatt aatatataaa 8040ttgcaagata agtcacataa tcaaatacta gccaattaaa tgctaaatgg tgtgctggta 8100gagcataaca gtcattcaga aaaggaaatg ctcattatga gctagagtgc tggggaagtg 8160gagcgatggt agcctcatag ggtgtttggg ttttagagca gttcttgaag gatcactagg 8220atttggaggc agagtgcaag gagtttagag aggtcaggct gtctgtggag taaagcatac 8280cctagaaagg tgaaggtgaa cattgacagg gtgtcaagct acgtgcgttg taggaagtag 8340cagggagcat ggggcactga ggcactgact ctgtattata ggtaggaaga ccagggaact 8400tgctagtgta taaaatccca gcagaaaaca accagattta gagaaggctt gtgcaaactg 8460cttaaaaaaa tgttttgccc agtagaaacc ttgtaattta ttgggtgcct actctatgct 8520aatctctcaa catgggaagt ccttgaggct ctattttcaa ctaccctgca tagactcatc 8580tggaaagagg ctgtgactag ggttggcaaa ggatatgtgc agagtgttta cacattgttc 8640tttctctcta cctttatgtg ttttctcctg attcggaaac cagtaaatga aaaagaaagt 8700ctggctattt ggagtaaatt aatgagctcc tagaggagat gggactagca gagtctgctt 8760gtaccaggaa ctcttagcgt cgatttcgag ctgttgctgc caaagtagca aggaccaaag 8820agtgagcgtc ttctgccatt caccttcaca atgtagtgtg acccatctgc tagtgtgttg 8880tggcatcatc aaccctcttc ttccgctcgt ccatgccagt cagcacatca gggagactta 8940aaacgtagta cctgggtcct gggccaacat actgttttca ttaggactga aatcatagaa 9000ttctttatca ggccatctta tgtctgcttg caagaataag cagacaaggg gatttttgat 9060ggtagttctg cattcatctt gatcctggct tcttttcatt gcatagaaag ttggctgcac 9120ctggtacctg gttgaagcca agtggtttta ggagaacaat acttggggga atgcatctag 9180tttgggaagg gagaagtggg gtggaaagcc aatcttacct acctacaatg ctctcacccc 9240accggagccc ttccccagaa gcctgagcct cacactctgt gttgtctttc tagtgggaga 9300agttgcagat gacgtccagc gagcgcagga agatcatgtg ctcagtgaca ttccacgtca 9360ttgccatcac atgtgtggtc tggtccttgt atgtgctcat tgaccgtact gctgaggaga 9420tcaagcaggg gcaggcaaca ggtgagttct caggctggag caagctcagc atgggggccg 9480ggatagctca caggagtcct ggggtcagag gattttccag ttagccaaaa acgtctgggt 9540ttccggagga gggacatcag ttaggaggca ctagcctcct ttttggcatt tcttttgctg 9600tggtggtagt ttttgaaacc cagtcaggag agttcatatt ctggagcatg agaatctccg 9660caaactacat cctataaaag caggaagtag ctggacactg tcttccactc ttctttgact 9720gagcaacatt ggatgagttg tgaatccatt ggtcagtggt gcaggcttgt ggtccccaga 9780tacttgaggc tgagggatgg aggattgctg aggctcagga gttcaaggct gtagtgtgcc 9840atgatcacac ctgtgaatag ccactgcact ccagcgtggg caacatagca agactccatc 9900tcttaaaaaa caacaacaac aacaaaggtc ttcaccaggg atctgcctag gtcagcgagg 9960tggttcatca agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggagtgt 10080acttcacccc gtggtgaaga tgcggcacag actggcgttt cctacggcgc cttcactgga 10140aagcgcccct gtggtcctgg ggagagggcg gctccatccg ccacccttcc cggagctgcc 10200cccgcccatt ctcatgggag gaaatcgcag ggcgtgtgag cccccgaccc ctcgccccca 10260cctctgccag gaccggccgc atttctgcac tgtcccgccc cactgggaag gaaactctct 10320ttctcaattt cctttaatga ggacattgtc ttttcagccc tgccaccccc tgcaatcgtc 10380ttccaggctg caaacagcga gtggggaggt gaacacatca gcttttcctc cctccacacc 10440accaggaaat ttcgcaaacc acttggctct cggcggcctg gcctcgccac acctgtgccc 10500acagtctctc tccgctgcag ccccctgtgc cattctccag gccccacgta cctctgcctg 10560ggcccagtaa gacctggtca gatggccccc ggctgcaaag ccttcctggg ccccatcaga 10620gaggacgccc aggtgaagcg agggtcccct gcccaccctg caagcgcagg ggcccttcca 10680agagtgcagc cctaggccgg tgtgtgatgg tggagacgcg gtcccagagg gcctgaacca 10740agaatggaga agacaaaggc tgtctttagg gagaaacttc cctgaggggc tgatgtgtgc 10800gatcccattt taatctcaca accctgggga ggagctgggg ccgggtctca ttgttcattt 10860gaggacaccg aggctcaggg aacttagacg actttcctag ggtcccccca acccccaccg 10920gcccagggcc ctctcccagg cacccctcat ccccacacag gctcctctaa gctgcttcct 10980tgtggtccag cccccttggc tcagctcctc aaatacaggg acacctcttc tctcccccgc 11040agaactcaag ggtctccatc cactgtgcca gcacagaggc ggtgctcaga gtggggctaa 11100gcttactgtc cagcctgctg ggccaggagg ggcagaaggg cccctctcag gattccttcg 11160ctctgctcct gccaataggc catcactcaa ggggctaatg acaggacgat gtggagaaac 11220gtgggctgca gcacttctgg ggccaagggc agggtgggcg gcactgggga gtacagggga 11280ggggacctca ctggggccag aggcttctag aagcttctgc aagaaggggc ccctcaccca 11340tgtcttggcc aagaagtggg gagggtgggt gtggcaaagg aaacagctag gtcagagaca 11400agagatgctc tgcaaagccc tgctggctcc ccacggtgga gcatcctatg catgtaggac 11460ctgagagatg gcaggggcgg gtgcgtccct atggatgtat ctccatttct gggcagagac 11520atctgggctc tcacctgcaa ttgagccaac caagaaatta tatttacttt ttagagcgag 11580cctccaacaa attatacaag cacttgagaa taagccctcc cactgtcttg agagatggag 11640cccagggagg cagctggaaa gatgctcctg ccctcactca ggagagatgg tggagctggg 11700agaggaggag gcactccctg ggacccagca gatggacttg actcagcaaa tagatgcaac 11760catatgtggg gggtggggag gcccagaaag gcattacagg ccagtgttct taatcctggc 11820tatacactgg aatccaccag tagccctaaa atataactgt gctggacttc ccctggttcc 11880acagtttgtg cagagtgggc agcatggagt ctgtaacact aaacacacag gattgagaac 11940catatgtcta ggctgttgtt agccatgatg gggatacatg aaaaggaggt gcacagttac 12000cttctatacc acaggatagg tgctcattaa accctgttaa aatggactgt gacccagctg 12060gcactcaggg gcactcaggg gccaggtggg tcacactttc attttaatag gatttaatga 12120gcacctattc tgtgacagct gctaagatac agaggctcct gagatacaga gatgacaatg 12180accttcagaa acactgaaca gagataagaa aggcacagtc tggccttcac tccaccatta 12240attcctccat ccatccatcc atccatctat ccatccatct ctctgtctat acttccatcc 12300atccaccatc cattcaccat ccacccatca atccatccac ccatctatcc atccatccat 12360ctcactatct gtcatccatc catccatcca ccatccatga atccatccat ccgtatatca 12420atttctcatc tatctctcca tccatccccc atccacctct tcttccatcc ttcctccttc 12480ccttcatcca tccattcatc atccatccct caacccatcc atctgcctgt ccatcggttt 12540gattgtccat ctgtccagta ttcctccatt ccctccaaac tgggtggcag tactctccac 12600ctcactacct caccatcctt attattccct ccaaactgtc tctttcacaa gggccttctg 12660tcctgcctgt cccatcctgg tctgggacct atcccactaa agctacatgg ctttgatcaa 12720caatcccaat cccagagtag gggaagggaa gtgagcccct aactgccgtg atgtttccag 12780ggagatggtg gatactgctg cattactaat ccagatgtta ggttttaatt tttctctcca 12840gtagacccca attgtggtag gagaaaaatg ccccagcttt ggacacaaac agacctggat 12900tcaaatcctg gttttgccaa tttctgccta aatgacttta ggcaaactcc ttgaccttga 12960ccccttggag actcagtttc ctcacctaga aatggtcaat aatattattt acctcccagg 13020atggccatga agcttaaaat agattattgt accccccaaa acaccgacac aatgtttaga 13080aataaataat attattttaa gctttgaagt ctggctgggt tctcagcctc gtctacctgc 13140tgggtgtgca gactcaaagc cagggtccca ccctcataga ctgtcatctg tgggtgtggg 13200gttggcctgg cactagcagg tgctaatgct cctcagagga ttctaggtgt aaaaggaaat 13260gaaaatcttt ggaccccaat tcactctgcc aaaagggaaa aattaagctg aaagctgagt 13320gatgcaagaa acgaagatag ctacagataa aaggtgaaaa cctccacagg tagctcttgg 13380tgttcacctt atcaagtgct gatgtactaa gcgcaagagg aatacataac tgactattcc 13440cctacctgct ccttttcttt tgcaacatgt ggattcagta acgtgaccat gccctccctt 13500tcttccttcc agcctgttct cctttaaata ttgatgccct taaagacatc ttcggaaaaa 13560ggcacagtcc accaactgtt cctgtggatt tgtgttcctt ttttccgtgt atgtcttttt 13620ttttattatt atactttaaa ttctaggata catgtgcaca acgtgcagga ttgttacata 13680tgtatacatg tgccatgttg gtgtgctgca cccattaact cctaattagg tatatctcct 13740aatgctatcc ctcccccctc tccccacccc atgacaggcc ctggtgtgtg atgttcccct 13800tcctgtgtcc aagtgttctc attgttcaat tcccacctat gagtgagaac atgcggtgtt 13860tggttttttg tccttgcgat agtttgctga gaataatggt ttccagcttc atccatgtcc 13920ctgcaaagga cattaactca tcctttttta tggctgcata gtattccata gtgcatatgt 13980gccacatttt cttaatccag tctatcggtg atggacattt gggttggttc caagtctttg 14040ctattgtgaa tagtgccaca ataaacatac gtgtgcatgt gtctttatag cagcatgatt 14100tataatcctt tgggtatata cccgataatg ggatggctgg gtcaaatggt atttctagtt 14160ctagatcctt gaggaatcgt cacactgtct tccacaatgg ttgaactagt ttacagtccc 14220accaacagtg taaaagtgtt cctacttctc cacatcctct ccagcacctg ttgtttcctg 14280actttttaat gattgccatt ctaactggtg taagatggta tctcattgtg gttttgattt 14340gcatttctct gatggccagt gatgatgagc atttcttcat gtgtctattg gctgcatgaa 14400tgtcttcttt tgagaagtgt ctgctcatat cctttgccca ctttttgatg gggttgtttt 14460tttcttgtaa gtttgtttga gttgtttgta gattctggat atcagccttt tgtcagctga 14520gtagattgca aaattttttt cccattctgt aggttgcctg ttcactctga tggtagtttc 14580ttttgctgtg cagaagctct ttagtttaat tagatcccat ttgtccattt tggcttttgt 14640tgccattgct tttggtgttt tagacatgaa gtccttgccc atgcctatgt cctgaatggt 14700attgcctagg ttttcttcta gggtttttat ggttttaggt ctaacattta agtatttaat 14760ccatcttgaa ttaatttttg tataaggtgt aaggaaggga tccagcttca gctttctaca 14820tatggctagc cagtcttcac ccggggctga aggtaccgct tnnnnnnnnn nnnnnnnnnn 14880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 14940nnnnnnnnnn nnnnnnnnnn nacaatgaga acacatggac acgggttggg ggggtatcac 15000acactggggc ctgttgggct atggggggct ggggagggat agcattaggg gaaataccta 15060atgtagatga caggttgatg ggtgcaacaa accaccatgg cacatgtata tctatgtaac 15120aaacctgcac attctgcaca tgtaccccag aatttaaagt ataataagaa aaataaaaat 15180aaataagtta aaagagatta aaaaaataaa agagaacatt tgccacattc agtctttcta 15240gatggaaaga ggttgctgac atatgataga attagaaaat cacacatctt gtaaattctc 15300atttgtttaa aaagaaatca tacaaattag atgttttttg gagatgactt tttaaaatag 15360agtcgttaga tcacctctgt aagggatatg tctatatctg ttcagtgggt taggggacat 15420ggatctggaa agcctgagaa gaaaaagaag gttctatacc agacttgtga tatttagaca 15480ttttcatatt ctatccattg ttttgtgtgc attttattcc tcactattgt atatatagtt 15540gacaatgcta aacttttttg tgtattcttt ctatgtgttc cgaatgccta atatatgtca 15600aaattagcgg tagtaaaata atattttgta aatatctttt tgctaaaatt catatgaaat 15660gttgtttttg gaggggaatg gccaaactac ctgttgagta atactcatcg tgtttgtgtc 15720ctggttcagg ggaggaggaa ggaggggaaa gtgcagagag ctctatgccg ctgtgtttac 15780agtgaggcaa gattaaccat tatctcttat gtctgtgcat tttgttttac ttatctgttt 15840atgtagtgta tataaaggac aaacgagtcc taatttacaa catctagtct ttttagatgt 15900taaagaggtt gccagtgtat aacaaaagta gagttagtaa actaatatat tttgtatatt 15960ttgttttaaa attcctagga aagattgtct tcctatcttt gagcattctt gcgcactgag 16020ttgatggaga tgggagggat tctaagctag gatgttctta tttggaagac tctttcaaat 16080tataactatg gttacatgta tgcagtttat tcgagactgc tgtgtatata gtggacaaat 16140taactcctta cttgaaacac ctagtttatc tagatgttta gaagtgcctg atgtacgtta 16200aatgtagagg taataaaata ccactttgta aatatctttt tgctaaaatt cataggaaat 16260actgtctttt agaaatttaa ttgttaagcc acctttgtga gcagtatagt actgtctata 16320cttgttcaat ggtttagagg aggtgggagg gaagaaaaat atgtgactga cttatttcac 16380taagcctaat gtctttaagt ttcattcatg ttgtggcatg tgtcagaatt tccttccttt 16440ttaaagctga ataatattgc attgtataga tatattacct tttatttatc catttatcca 16500tcaatagaaa cttgggttgc ttccatattt tagctatcat gaatattgct gccatcaaca 16560tgttgagttc ctgtattcaa ctctttgggt aatacccaga agtgaaattg ctgaataagt 16620atggtaattc tctgttaaat ttttgaggag ctgctgattc atacttttac gtataaaata 16680ttagacctta aatgtattat gtaaaaacac taagtatttt taacattcta gagtaaagaa 16740ggtgtttctt agcaagacag aaaagctgaa accctcaata gaaaagcctt gttaatgtta 16800tcacataaaa tattcaaaat ttctgaacac acagacacac acgcactaac gaaaagctat 16860ttacaacttg tggtagatga agaaataatt tcttaacata gcaagaaccc aggcaaatga 16920ttaagaaaaa gaataatcat acaaatttaa tacatgtgag aagctgttca gcctttccca 16980ttaattaaag aagtaaaata taaaacaaaa catataattt ttcacttagc agagatgaaa 17040taccaaaaca gatgggtata ggtgtggaac atacttcctt ggttgacagt tagccaatat 17100ctattaaaat cccttgccac agtaatttca gttctagaca tttgtcttat attactgtat 17160ttattctgtc ctgtaaccat aaattccaca gctgcctagt gatggttcta tatttaaatg 17220gaatgcttac tctcagtatt gttttttctt ttgagatgga gtcttgttct gttgccaggc 17280tggagtgcag tgccatgatc tcagctcact gcaacctcca cctcccaggt tcaagtgatt 17340ctcctgcctc agcctcccga gtagctagga ctacaggtgt gcaccaccac gcccagctaa 17400tttttgtatt tttagtagag atggagtttc accttgttgg ccagtatggt ctcgatctct 17460tgacctcatg atctgcccac cttggcctcc caaagtgctg tgattacagg tgtgagccac 17520gacacccagc cagtattttt ttttttaaac cacagcttct tcattttgag tcatctctaa 17580atagctgcaa tttactataa cttagggttt acaattttta aaattgtgta tgttaaggta 17640tccacatgat gtttgatata cttcttttga cataaatata cacaatgaag tgattactac 17700agccaaataa tgattaccat acccatcatt gctagcgttt agaaagacaa ctgattttta 17760tgtgctgata ttgtaccctg caaccttact gaatttacta aatctagtag ttttttggtg 17820catcctttgg gattttctat taacagaatc atgccttctg caaatgtaag taatttagct 17880gcttcttttt aaatatgtat gcctcttatg tctttttctt ggctatgttc ttttctctgg 17940ctagaacttc tagtacaatg tccagtgtca gtgttgaaaa tgggcatcct tgtgttgttc 18000ctgattttag gagtaaagct tacagttttt ctacccctga gtatgatttt agttgtgaga 18060ttttcctata tgccctttat catgctaaga aagttccctt ttattttagt ttgctgattt 18120tttaaaatta tgaaagggca tttaatttta tcaaatactt ttttctgtgt ctgttgagag 18180tttggccatt ttttttctat tgaaatggtg tattatggta gctgattttt ggatgtgaaa 18240ccaaccttgt gtccttggga taaatcccat gtaatcatgg tgtataattc tttctatgta 18300ttgctggatt tggtttgcta gtattttgtt gggaactttt gtgcccataa gataagaggt 18360attgatctat ggttttcttt tcctgtgatg tctttatttg gtttttgtgt cagggaaata 18420gtggcttcat aaaatgttca agaagtgtac cctcctcatc tttttgttgg aagagtgtga 18480aaaattggta ttatcaaatg tttagtagaa ttcaccagtg aagccatttg cttttctttt 18540tgggtagttt ttgattactg attcattctc ttaatttgtt ttaggtctat tcagattgtc 18600tgtttcttct tgagtcagtc ttggtagttg tgtctttctt gaaatttttt catttcactt 18660aaattatctt atttgtcagt gtacaattat ttttggtatt cctttataat cctttttatt 18720tctttaatgt cagtagtaat gtctcttctt ttatttctga ttctatagtt tgagttttct 18780ctcctctttc cctgatgaaa ctgaagtttt ttccatttta ttgatttttt tcaaacgatc 18840aaattatttc attaatttgt gctgttatca ttatttcctt ccttctgctt acttaaattt 18900agtttcatat tttttgtaag tgtcttaagg tagaatttta agttattgat ttaagaactt 18960tcttaataca agtgtttaca gctgttgata ttactctaat tacttcttta gctgcatcac 19020aattgttttg gtatgttgtc ttcattatta ttcgtctaaa aatattatct aatttctgtc 19080ttgatttctt ctttgactca tgaattgttt tagagtggaa atttcatttt aaatttccaa 19140atatttgtga atttcccaaa tttcccctgt tgttgatttc taattttatt ccattgtggt 19200taataacata ctttgcatta tttctatcct tcaaatttat tgaggtgtgt tttatggcct 19260aatatgtggt ctatcttcta gaatgttcca tgctcacttg acatgaatgt atactttgtt 19320gttgttgtta agtagaatgt tccatagtcc ttgaggtcta tttggtttgt agtattgctc 19380aagtcctctt tttccttgtt gatccgtcca gttgttctat ccatttttga acgtgtagta 19440ttgaagtcct tgactatttc tgttgactcg gttgacttgt ctatttcttt cttttctcag 19500gtctgttttt gtttcattta ttttggtgct tcatatatat aggtgcatat atgttgataa 19560cagttatatc ttcctaatgg atggattcct ttgtaattac aaaaggccac tctatctcta 19620gtaacaattt ttgttttaaa gtctatttgt ctgatatcag tgtactgact ccagtgtttt 19680tgtggttgct gtttgcataa tatatttttc ctgttctttt actttcattc tgtgtcttcg 19740aatcaaaatt gtgttttctc cagacaatat atacttcatg ttttcttatc caatataaca 19800cctttgcctt ttgattgcat tgtttattcc attcacactg catattatta tttagtggga 19860tttatgtctg tcattttagt tcttttttgt ttttgttttg agacagaatc ttgctctgtc 19920acccaggccg gagtgcagtg gcgtgatctc ggctcactgc aagctccaac tcccgggttc 19980aggccattct cttgcctcag cctcccaagt agctgggact acaggtgccc accaccactc 20040gtggctaatt ttttgtattt ttagtggaga ctgggtttca ccgtgttagc caagatggtc 20100tcgatctcct gacctcgtga tctgcccacc tcggcctccc aaagtgttgg gattacaggc 20160atgagccacc gcacgcccgg attttttgtt ttttatatgt atcatgtctt tgttgttcct 20220gttttgtctt tacttctttc tatttcattg aattaatatt ttctaatata gtatttaagt 20280tttattaata attttatcac tgtattttta tttttagtgg tttctctagg attttctgta 20340tatatcttat cagtatgagt ttcagattca cactagctta attccagtaa tatgtagaaa 20400tgttactcat gtagaactta attctcttgc tcttttgtgg ttattttatt tatattatat 20460caattaatgt tacaaagata tacattgttt aaattattat tggtgctatt attagcatac 20520ataacaccca catattaaaa ttgtatatat tgcatatatg tattatagaa ttgtatatat 20580tatagggcca atagtatatt gtatacatgt tattttatat aattgttttt aaaatgagtt 20640aagaaaaaag aaaaatgcat ttctactgtc tattatagaa aaataattac atcatactga 20700acgtcttcac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 20760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcggaatt 20820cctaaaagaa ttgctgagac aaagtatatg tgcctttgtc atcctgaggg cagttgtcat 20880cctgtggagg tttaccattt gctctcccac cagcaatgta taagcatacc tgcttcccaa 20940cacttccgcc aacacatatt atttgattaa ttttcttggc tgataatggt atctcattgt 21000agttttaatt tgtatttctc attgtgagag aaatgaagca tcttttcatg ttttgagagc 21060catctgtgca tcttttccca ttgtttctgt taagtcattg atttgcagta ctgtaatttg 21120tgatgagttg taaacatgag ctcttctggc ctcttaacca tccttatcca gcctgtcttc 21180acgttcctcc ttgcctaaaa acaagctcac tcaaacagat cattcctgtg gccctctccc 21240ttttgaaata aatcagatgg gcaaatttgc ttttgttagg ggcctgagac ccaaatgttt 21300aaaagtagct tagctgatga gtgaaaagga ggcccagttg ggggttgcac agaaaatgag 21360gggcgtgggt aatagaggag gctgggcagt aacctggcag aaggcgccag aggccaagga 21420aggtgcgctg gtgtcctgca gtgagaagga tgtgtgcact atcaactggg taaacacact 21480gctatagctg ctccagcgac agcaggcact ctcagaaggg aactgagaaa aggggaatgc 21540cagtttctgc caaggatata tggaaaagtt ttttttcccc gtatgttctt tggcaacctt 21600gttaaaatca tttaaaatgg tgaatttgat aacaaagcca gccactaaaa gacttggatg 21660tagcaaaaca aattgtcaaa aagtatttat catttctaag aaataaaata aactgggatt 21720taggcttgga tagaaatcca gaggtttgtt ttggcataat taattacaac atctgaagaa 21780gggaggattt ttacaagaat tttttttttt gtagtgagat tgctttatat tccttttcat 21840gactggagag tctgtcattt ggcaacccgc aaagaacttc aggaacagag ggcctaagtt 21900ttcttgacac atttggattt ttgcatgatg aacatttgca cttttattag taattgtcga 21960gtcgtgtgtg gtctgatcct atggagagga gagtgtaggt gtnntctctg gagctagtac 22020ntgcccccgn gctcacacgt gccacactgc ctgctgcttt cangaaagag caggacggct 22080ggtcctgtcc catctagtga ggtcctgtcc catctagtaa ggttctgtcc tcagagctcc 22140taagagggga ttgctggcct ttgattacac tggcttccag aatctgacca tggaaccatt 22200atttgaagct gtcagcttca ttttgttgtt gatcaggcca gtcttttagg tctttgttgg 22260ttgatagatt tctctcaggt ctatagcagc aacaaataaa gctctttaaa attattttct 22320tgaccattct gcctatggat tggttctgac ctttgaaatg tttgctgtcc tttagatcgt 22380gacagaagga gcctcttctg ggagctgtga agaccccaca gatctcactg gttttcttgc 22440ttttattgga agagttagca tttgttgaag tttatattta tgtaaaataa caataagtag 22500attttaaaat ttttctttaa attctgtgat ctaataagaa gagagactct ttctctgtca 22560ttgggaggct tgatgaggat cggacatacc tgctgagagt atcctgtaat gttttcataa 22620gattggaaat ggtgaaatga gagcagaaat gtaggcagag gatgccaacg ctgtttccta 22680tttcctaagt aaaactgagc agggcagaat ctccccagcc ctgccagaac tcccttcctc 22740cttgggtccg ctgtcactgt catgctggcc ttgacatctg ctacaagtgc acacacatgg 22800ggaaccacac agggaagtga acacatgtcc tgacgtgtgt gtgtgcctgg tgtggctgct 22860tccttgatcc aggctttgtt ttgttacgag gctctgccct cctaccttca ggccttgctc 22920ctcatcatgg gcatgcaagg atggaatcgt taccccagag tacacttttt gacttggatt 22980acttgtagtt tagtactgga gttcagaact ttaatttccc atcagaaacc tgcctcccgg 23040acccaggaca ctgaccttga ttgggcacga ttttccagat aagaccttac ctcctcccaa 23100cagggtttgc cttcccttca gaatacagga aagtacacgt ggttccaaga ggaccatgct 23160cttactaaga taaaagggag gagggtgact aggtactttc ctctgctgcc atcccttgaa 23220ggaagggggc ttccttcaat gcctcatcag ttccagggag tatgcttgac ccagcaacac 23280agaggaagaa gtgacctcat agcaatgagg ggcaagcatg tggatattgc tgggtatttc 23340ttcctcaaag tctacctggg gacaggtgat gaagacgctt acttctctgc aatacaccgt 23400gatcaggctt tggtcttcat gacacatttt agccttgcct atgtgtgttg aacagagctg 23460gtcatcctca gaaataaaag aagagagcaa taagtacagg aaggctttca gtcccagaat 23520agtgttagga ctaaatgtca gagaacaggg ggactgtagg agtttgggga ccttagcatg 23580gataccctct caatctgctc ccctgtggta taaacagtaa agcccattgt actaaaaggc 23640cttgagtgct tgtctctggg gcaggtggag ccagtgatgg gtgccccgag aggaggatgc 23700tggagagggg acagtgtcta aggcagttgg agaaaaagga caatggctta tgcagtcagg 23760cctagaagat gcaagggatg tcacagcata gtgtccacag gcatccctag atgtctttca 23820ttttgtttca ggaagaatat tgacaaaggt agtgatggga gggttatttc aaggatgcta 23880ttttcatgtg tccaagtaaa cttaagattc ctaagaactt tgacctctaa tgcttttaaa 23940gaccgtgtac ctcatgccca tatgcacatt cagttagaaa ataaatgtag attctttggt 24000gaattcgaag atgtgctcat ggagtggcac agcgtgatct cagtgtacac atgaagcatg 24060ctgatgagag cttcttggat cttgtttctt caggtggact tgcattaaag tgctgttgaa 24120tctctgcact gggagtggga gcagccatgc gatgggtgcc ttaatccgag tgccatcctg 24180gctctctcca ggctgcttca tttgctgtga ggggtagggg gaggaggagt ttccaggcaa 24240tggccttatc tgttctggtg gttgaccaag ggtcgggggt cccaaaggtg gagttccagg 24300atgagagtgc acccttgggg atggtactta gaaagagtgg ggactaggcc tcagccaggg 24360aatggtgggc cagggtggag ctcctttgta tcaaggagtt agggccaagg gccatgcagg 24420tgggcctgca gaaggtgggt ggtgatatgg cctttctggt gataacagac tcgtggcact 24480gttttcctgg ggctgttggg gttgcatgga gcagattctt actcaggctt actgtcttaa 24540ggagggaaga atgctttttt atgaatacgc ataggtctgt ggggctgtgg agcctgggga 24600atccgaggct ggcgtgggag tctggggccc tctagcggtg gtggtgcatc ctcagctcag 24660tgccactcac tgtccctatt tctctctgtc catttctctc ctcactgcag tgcttcctct 24720ccttcctaga tttttttctc taagtttgac tattcaccac cccagagact ctgttctatc 24780ccatgacctc ttgctgtact ttctttcaaa tctaaatttt cttgattatt tttcttaatt 24840tacagtttca agattagatc tgttgggagc attctgtgct ctacctcagc ccctggttga 24900gctatggatt ggtgtccccc atggtgacgt ggtctggact cccttttgtg tgaagagctg 24960tggcagggca gtgaggtttt gtggaactca gggagcatct ggcactatgt atgaggttgt 25020gaggtgggag agagctctta aatctaccag ccttgttgat cttgggaact aggagagagt 25080gctagggaag ccagtgccag caggcctgga gcctcatatt tctttggcat ttgtatgata 25140ccttctagta cttcgttttt tgacttatat atgaactaac tcaatttaga tgcttagaaa 25200ataaacggcc actggacctg caagagatta gtagtggtgt tccatagcct tagacatttc 25260ttacactttt atccacagat aagaaaaaaa tagtgatatt tgatttgttt tgccaaggct 25320tagaaggatc taatatgtgt tactcgttga gcagcgggaa gcagcagaca tttctagggt 25380ttctccctgt caccattcag gagacagtgc acacaatccc cttggctctt acctgaacac 25440tgaccacagg tctgtcattg caggctggga gtcctccgtc agcatcagct ccggctccgg 25500tgtcctcctt ctctcgcact tctatcacgc catccagcca ggacatctgc aggtaacact 25560ctgcttcaga tgctgccatg atcagtgtgc agccagaaat aagcattagt gtctgtaggt 25620tcttgaattt ccatttgctt aatcaatctg cctttggtct aaacagttgg cgagttttat 25680gtgtggtttg ctcaccaagt tacccatgct agtctcattt cttcactatg atgtctattc 25740tcttttaaaa ttgtttttac tttctgtgct tttatcccac tgttctgcat gcagttccag 25800tgcagtgttt tctgagtgtt gtcaccacag ttccgtgcag tctgctgttg tctcgaaagc 25860tcctcactgc cagagttctc tgacacaagg gctcactgtg acagttatct gtaaggacac 25920attacaggcg tcaaagagaa attcctttgg ttcagaatgg gcccaggcct tgaagcctgc 25980taagaatacc aaagccagaa gaacactaaa gttctcaagg tccctcaatg atgtgggtga 26040gaaggcgcag gatacttcag aaagttttgc ctatgtggaa agaacttgtt ctgaagggaa 26100attaatactc cctcaagata cgtgtctcag aactaacagg tttcatcata aagaaaaaag 26160aaccctgaac cacaaacctc ttggcaattc caaacattct tgtgtttcat gcctttctgc 26220cggtcgctca actgcctcag aggtggaagc tggcaagggg ggcaggcccg gcctgctgct 26280ggaagagaag gcggatggtg aggccacgtc ccgaagccgg caactgctcc agtacctgtt 26340ctcactctcg cacggcttga gcgccagcag cctgcacagg ttccatgagc tggagagctg 26400cgctgctcgc ctgcacactg ccaagtcctc cagcgggctg gcagggagta tgggcttctg 26460ctctgacgag atgggagacg acgatgtctt tgaggacagc acatctgcaa aactgaagag 26520tagggttctg cgggcgcccc tctgctccac ggaaaaggac agcgacctgg attgtccttc 26580tcccttctct gaaaaattac cccccatatc tcccgtgtcc acgtcagggg atgtctgcag 26640gttggtttgc caggaagtgc cattctagcc atctctgctc gcatcaggaa cctcctcttg 26700ctttagtatg tgaagtttca ggaaaatgaa gtaataggaa gttgtaacca gttgtgggtt 26760gatggttttc tccagaagaa tggagtatca atatcgtttg aaaaacttaa tttttatgat 26820gtgagtccca agatagtttt acaaatgata aaagaggaag aagctggctg ggcgcagtgg 26880ctcacgcctg taatcccagc actttgggag gctgaggcgg gtggatcacc tgaggtcagg 26940agtttgagac cagcttggcc aacgtggcga aaccccgtct ctactaaaaa tacaaaaatt 27000agccgggcgt ggtggcgggc gcctgtaatc ccagctactc aggaggctga ggcaggagaa 27060ttgcttgaac ccgggaggcg gaggttgcag tgagccaaga ttgcgccatt gcactccagc 27120ctgggcaaca agagtgaaac tctgtctaaa aaaaaacaaa acaaaacaaa aaaaaaaaga 27180ggaagaagct acacatatta aaggccaagc tgactcttct aaactaaaaa taagtagact 27240ttgcataaag aaactggaag aaagaaacta gtatcttcag ttttccagcc tgatggtcca 27300gattttaagc attgttgcat ctcccagcct cttttttaac ccttgctccc tacctacccc 27360caaaaggtgt gtggggactg gctgttctcc agtagctcct tccttccttc tttttaactg 27420aggtgtagcc acctatgtcc catcctaact ctgtttcccc cctttatctt cccttgtgca 27480gcatccttgt ctggcctcca taattcactt tttgtcatga ctcttgtgcc ctcctcattc 27540tgacctttct ccagtctccg ctccttagtg ccagctgtca tcatttctaa catttggatc 27600agagatcccc tcactaaaac agcctgagag cctggctcct tccaggacga gtgccccaac 27660ctgttctcta gctgctggaa aagacccctc tgtttcttaa aagcctgtta agttccttca 27720ggctagtagc tacaccttgt tgcatacttc cttggaaata gagagtgctt tcaaattaat 27780ttctcactta ttgctattag tggtcccaag aaaaagagat gaaattgcag catcttgtaa 27840tggtgagttt tttaggtcta ggagagaggt actgtcatct ttgtttactt gctgagagac 27900gacagaaatg tgtagttgtg gggtatgaag gtaaagtcag tgattatagc taaaaagcaa 27960acgtcattta aggtcactgg tatttttgga tgttggaatg cagcgcttac catccataca 28020agaacccgct gggagggcca agaccatccc gtgcgcggcg ggaaccgcga catccgccac 28080tcggatgccc gctggagcgg gcggagaccc acgcgcgcgc caatgtcccg gcgcgcgggc 28140tgctcaccca gggcagcggc gtagtcgccg ggggcacaag cccgagccgc ccggggcggc 28200gcgcggagaa ggagcgcaga ccccgtgaac acgccggagg accgtccgcg cggcccgtcg 28260cgccactgcc tactgccagg ggcggcgggt gacgacaaaa ccctcggtgc gccgcgcctg 28320gcagtccgcg gcccccgccc ccccagcggc gcgcacccgg gggcgcgggc cgaacccggc 28380aaggtgagag ggccgtggcg cccgggcgct gaccactatc attctccgcc acacggacga 28440aggcaggagc accacgggcg gccggcgggc cgcccgctcg ggccaaagag cccgcccgcc 28500agcggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagg ccgggcgagc 28620gcaggcgggc ggagagcaga ccacgcggaa gaggccgaga gagaaagcgg ggcacccggc 28680cgggggggtc atttacaagg gacgctgggc acgaaggagg ctggggcgaa gatgagtcca 28740ggggttgggt tgagagcgct gcgcagccgg gctgagaaga gaagctgacg agtgactccc 28800gacaagggac gggggagagg gaacctgggg gcaggcagag gcctaggggg tgggctctga 28860gggcagccac tcccaccggc cctcccccag ccccgcaggc cagccacatg ccccgtgctc 28920cagacgaccc agctacatgg atggcagcgt cctgaagggg gatgatcggt agggagtggg 28980tgcggaggct cacgcctgta atcccagtgt tcggggattc ccgagcggga ggatcgcttg 29040agccggggag gtggaagctg cagtgatctg cgccactgaa ctcccgcctg ggcggcagag 29100tgagaccctg tttcaaaagg caccaggggt gtagggaagg agcgatatcc cccaggcaag 29160gaaggctgcg gaggggccag gccaggagag aagggccctg agctcagtgg acgggcaggg 29220tgcctgtgga ggaagggagg gacgtagcag caggaggaag ggtgaggacc caggctgggg 29280atcgggagaa gtgatggcgg aggaaagaga ggccggaaga ggagggggcc tgctggcctg 29340gcactggaaa gcaaagcgtg ggtttgtata tgggaagaag accacatccc ctcttatcgt 29400catcccaact cagaaaccat ttggccccaa gtcaccaaag cactcatagg aaccactttt 29460cctgtgacac aagtaggatt ctcccacttt taacttccca aatgctaccc gaaagcctct 29520gaaatccttg gaaatttaaa aaataatccc cttctattaa ctgatgccta taaaacatat 29580tgactttcat taaattttgt tatgaaatca gggatgtgtc ccattgtaaa ataaatttgt 29640ttggaaaatg tagggtggta tcccatctaa aaatcagacg aggctagaaa atttcaattt 29700tatttgctaa ggtagcatca tgttgttaaa aatgcatcat aaaataagac caagagttaa 29760tacaaagcat gaagtcatgt ttccctcaaa aaaatgattt gttctgtagt caaattaatt 29820tggaaaacat tgcaaactct tcagcagtgg ttctcaaagt atagcttgcg tcagaatccc 29880ctggggccct tgctaaaatc tggatggcta cactccccgc ctagagtggg ttaaactcag 29940tgcccactgg caggaaactg aaacttggct gaagtcaccg aggaggcctt ccccgttaaa 30000cctctggctg gcgacagctt ggtttcctcg ccgctgcttc tgagtaattc tggcacgcag 30060ttcctcctag ccacctgctg tacctggaag ggggccagcc ctcctcagca ggctttgctc 30120ctgccctgca cagcacaact gtggcgtaaa aaatggaaaa gctggtagca gaaggaaaga 30180gcagggagac agaggttagg tgaacctcaa tggcctcatt aaagcaaatt gagaaagaga 30240gtttccttcc cagtgaggcg ggacagtgca gaaatgcggc ccgtcctggg agaagtaggg 30300gcgaggggtg gggggctcac cgggcacatg gccaacagct ggtgcccagc ctgggccaca 30360gtttggcaca ggagaaagct gaagatgcag gagaaagcta ggtcactgcc cctaggccag 30420gtgatcaagg tggctgttac ctgcctgtgt ggccttgaaa aagcctggtg ccctctctgg 30480cttgcagggt ccttcttttt acaaaagcgg gtcttcgaag ccctgaatat tccagttatc 30540tgtaaggata tagaaatatt ttgcctctag atgacatgta aatatgttcc tcctaactcc 30600acttcttcct acatctgtgg cttcttctga gaactaccag acccaccctg ttttcagagt 30660aaacctcctg ttttgaggtc aaacagaccc agctgggtaa ttggtgactc actcctggtt 30720cctccaaaga acaggggcaa gggaccaccc gggacctcag gtgccccagc acaggacctc 30780tttctggggc accctctggc actgggccat cttcaggccc tacagggctc tcggggcctg 30840cgtaggcctc tcatctccca gaaataagtt cttttcccca cccagactga caccagatgc 30900tacagtgagg tttacccctc cagcttgaca agcattccta gcccagaaca ctcagctgac 30960gaggggtgct gggggaggaa ggaggcagca aggctgaaag aagcttctct gggtattcca 31020gtgcctggct gctggtcgta gccatggtga gaccacagca gcctccctgg aagcaagcac 31080ctgtgtaaaa gcccctcctt ccccacaagg ggctggatcc aagtgaagaa ggtaccagaa 31140ggactggaag atgatgctgg tattattcga cagctccagg gtgctgtagc actgagcacc 31200tactgtatgc agcccatcac aaagccagcc acactgccca ggctgtgcca tggcaacttc 31260cagcagtaca ccctctcggt cacctgcagg ctccttgttc atcctgacac atgaggagaa 31320aaagacagta ctcacaaagg cctctagcct tgtggcccca ggaccctctc ctgagcatca 31380tggcctccta ggagacccag ggtagccagg acaaaggaga gaagcatttg ctaagctaag 31440aaaggatgga aggggattgc tgttctgcct ggatgcaccc ctagacctgg cagggcaggc 31500aggagagacc cccagtgtct cttgcttatc ctaggagttc ccccctgtgg ttgctccagt 31560ttgctgcctt catgcatgtt cacagggtga ttcccctgag acaacagagc tgggatgtca 31620ccaagccctg tgtcccatca gccaggctct ccccagcctc catcctctca ttctgctgga 31680tcaggaggat gtggggaggt ccctgactcc ccagctttcc ccatgacaag gtgtgtgtct 31740acagctcaga cccagccatg ctccacctga gaagtgggtc caggcagagc actgggtcct 31800ggccacctag ggctgcctcc tcaggggtgg gcaggaaggg gagccactgg aggagtgccc 31860cgggcacagt cccctgcacc aggtgaggtc acctcttagt ccatgaggcg atgctgcatg 31920agcccctctg gacactagaa ggcctgggct caatgctgag taaagtgcag gcatgactgt 31980gccctcagca tgcatagggc agcaggcata gattcataca cgtgagtttg cacttaagct 32040gcagtaagag ctacagagga aatgaacaga gacagcatca ctccagtgag ccctccagat 32100acaccccatg tgcgcagtaa gagaatggca tagtctattg ggacagccac atcaaaatac 32160cacagaccag atggcttaaa cggcagatat ttgtctctca caggtccgga ggctagaagt 32220ccaagattag ggtgccagcc aatccacttg ctggtgaggg ccctcttccc agcttgcaga 32280tgtctctgtg ttcccacagc atggtcacgt ggcagagaga gggggacagg gcatgaataa 32340gccctcggtg ccttcctctt cttacgagga tgctaatccc atcacgggta ccccaccctc 32400gtgacctcgt ctaaccctaa tcacctccca aaggccccat ctcctaatgc catcacactg 32460ggggtcaggg cttcaacaca tgaatttggg gagcacaatt tagtccacag cagaaaagaa 32520aggggcacag agcattccag cctgaaggaa acagatgtgc aaaggcccaa ggcaaaggcc 32580tgagctctga ggggcaacca gagtcaaggc tgaagagtgg gcagtgattg agcactgacg 32640gtggagaggc aacaggctat cttgttaatc tcagtctttt gtgtgccaga gagagaaact 32700gaggcacagt gtgtcatggt gaacactggg cagcactccc acgctcctgt cctgcactct 32760gcagggttat ggaggaagcc tgggaagtct ctcccctctt ttccaccttt cctggaccca 32820tcccacaccc tgcctcctcc aggaagccct ggggattgct catacactca catctcactc 32880cttagtacct gcagcctgca ttctgaattt agcaagctct ggccttgggt ttctattttc 32940ttgtcctgtg cagtgagcat ttggaaactt ctcctcagca ccaaacacgg agccatttag 33000cagaaaatac atgagttttc aagtcaattg aactgttcat cgccccaaaa gccaggcaac 33060acggtagagc agctggatct ctgttcgtag aagctttctc agggactggc ccaccgagtg 33120ggggactgca agtgcccgat gagtgaatga atgaacacat gtgtgaatgg aattcaaggc 33180actgcaggct attctggagg gtactggcct gtctcttcga caggtgaggt gcagtagaac 33240agcatcccgt atagatgcca gtttctgacc tggttctgaa aagccagtca cctacctctg 33300cagagctgcc tggagggggc gggggttgtg aggacccctg agagcttggt gtgaaggggc 33360tctgcagagg gaggcatggc tagtgtgcgc aaggtgacct ctctcctggt ctgcaggatg 33420gagaacctgt tcatcaaccg cttcatgcac atgttccagt cttcttggaa tgacttcgcc 33480gactttgaga aaatctttgt caagatcagc aacactattt ctggtgagtg tgcctctggg 33540ggcccaagtg gtgctgggga caggggatgc tgctctcctg tctgatactt gccgggaaat 33600tgacaagggc cttcctgcct gctgcagcat gggggcctcg ctgccaccat gccaccctgg 33660acagcacccc ctacactcca caccttttac catcactgtg gtcacccttg tgcccagagg 33720tttctcctcc tcagatattc aagctcgagc accctcaacc tcatttcact atcaaaagtc 33780tcaaaagtat cagaactttt tcttggcaca aatcaatcat ttgtgcttct gaacagagca 33840aaggcagaac ttggcttgtg ttttaggtat ttacacaaaa gcaactttgg gttcctttgg 33900actaggatgt gtttgatgag agcactagct tcatagtggc cagtcatggc atgggaagga 33960cgttaggaag gcaggaatgg ctggacaaga agaggtgctg aaatcaggac atggggaacc 34020aaacttggca aactgccccc agggactctc ctccacgccc tccttcttcc atcctggaca 34080ctccatgctg ggctgaagga cctgagtgct ccaggaggct tctgagccac gaagggccag 34140ctcaggtatg atgagttggt gccacagcct gacccagtga tgggcagggg gcagcagttg 34200gagctgtgcc catccaggga gcactcggga gaggagacca agcagggact ctgctcttag 34260gtgaggtcag gagggccatg gccctggctg ccctctactc agagctcagg gtgggcctcg 34320cttttctcct ggtagagcgg gtcatgaatc actggcagga agacctgatg tttggctacc 34380agttcctgaa tggctgcaac cctgtgttga tccggcgctg cacagagctg cccgagaagc 34440tcccggtgac cacggagatg gtagagtgca gcctggagcg gcagctcagc ttggagcagg 34500aggtccaggt aggggttgat gggctgggga agtggccaag gtcacagtct gtcaggtgga 34560agccagttcc tcctggccag tgctcatagg ccaccaagac gctaactgca ggcccatctg 34620gcctacagca gccgcttcct tttcctggca gcagtgtcag ccagggtcct gggcattatg 34680cagactgtct tgtgcaacat cagaggagga attgcgggga atgtttctcc atgatgctcg 34740agtctgggaa cataatgtca atattttcac tatcagtatc aataattaca ggagctaccc 34800ttgattaggg gcctgtggtg ggacaggcat tgtgacaggt gctttacaca caaggtcatc 34860agttgtcacc caccttgcaa aggggagaac actggagaaa gaagcagacc ttgtgtgaca 34920atacacaagg ggcacgagga gaacccaaca gaatggttga ttttctcgtt agaaatgctg 34980ctgcccatgc tcctgtcgca ccctcctccc caccctcacc tgcagactca gctgcctccg 35040gctgcaaggc tgacctagtc ttgagacaga agaagttcaa accaactcca cctggatctg 35100gtggggctca gcaacaggcg ctggcccatc agcctgcctc ttccccatgg atccagcgtg 35160atggggcctc ctgccaccca ggccctgctg cctatcccca ggtgtccagg tggcctctgc 35220tgcttccagt cttttgagct cagcttaccc tgaggcccta gttggagagg gatggttggt 35280gccctttaga gataaaccta cagccccagt acctggccca cctgaatcta gagggccacc 35340cacccagctg aagtccctgg gcaagtctcc tgccccagtg caggcagtgt ttcctaacct 35400cctcctcctc ctgcacccaa ccaggcccag aacacttcct ggtcatgtga acagctgaca 35460gttacaatcc cccacacact gagagagcat ctgtgttcct gagtggactg aattgctaga 35520tgtagtaaca ttgatttggt ggctataggt ttcaaacttc aagtcagaga tcatagaact 35580ttaagccata tagaatccta gaattggaag actctgagca gccatctctg cacccagcag 35640ccaaggagtg gccatcctca gcacctggca gggcatcagc ctcccactgg gagtcccggc 35700cacctccagg gcacgtgggt tatgagtcac ctttcctttg agctgaccca cccctcactg 35760actagactct taaacacaca ctgccccgtc taatacacac cagcatgatt cttaatcccc 35820acagtcattc acgctgccca tctggcagca ccttaatgat ctacagcaag cttgtggcct 35880actaaaaacg cctccattgt tttttaaaat gtgcagttat tttccggatc atgaatagag 35940aaattgtatc cctgttgttg gtattgattc agtgttccag ctcaagtttg agttcttaga 36000ctttatattt ggattctttg gtttcaaatc acaaaaatca actccatctg gtttaatcca 36060agaactctat tggctcaggc aacagtaaaa agcaatgcta actcatttgg gaccagggct 36120cagatcatcc cctgcactgt ctctctctcg ctccatgtct caatcctctg cctctggtga 36180ttttttcctt aggagggttt ccttatgtgg aggtaaaaaa tgatcaccgg cagcttcaga 36240tttaccttcc accagcttag caaacaccac ccactcccag caagaaagac cctgtttccc 36300aatgctccca gcagatgtct taggccatcc tcgggcctca ctgattctgt gagccagtgc 36360ccacggccag gagaatggaa tacactgacc tagaggggac agggactcta gacaggcaaa 36420aactccactg tcagaagcag aatcctaaaa tgtcagaact gaaaggtagg cccgcccctc 36480gttttccaga aatatgaaag ggaggcttgg gataacagag aaatgctgca ggcattggcc 36540ccaaatctca ggcctcctat tcccagtatc accttcacca gcctgggtgg tctcaagatt 36600ggattagcag ccattccttc tgggtagagt ctgatggtca cttcagctct caaagtccag 36660ctatccagcc atagtcctag aaggcaagac atcactgtgc ccgaccaacc caagggtctc 36720cacctgcccc cctcaacaca aggtgccctg agtgcctccg cggggatagt ccccgtcccc 36780acccctgctt cccccgccca tcgcgacaga tgctcagagc tcatccctgg tgtcacacca 36840cataaaggag gagggcggtg gccaggctcc gagctcctgc cgtcctgggt gggtggcttc 36900tgtcctgcgt gccgccgggc agcccggggg tcctgcgcgc gggcgcggtc ggtggagctg 36960caggggggcg ctctgggatg tctccaggca gagtagatac tgacccctcc gcagccggga 37020ccctcaccct ttccttcatc actcaggaaa cagaaaaggc ttcagaagga gcggccatgc 37080ccccggccta agcgctgcgt tcccggccca gcaggcctac cctgcacctg gcgttcctag 37140gggcccctct gcgggaccca ctgcgcggct cgcagcggcc gcccgcctcc cacttcaccg 37200gcacgctcgt tgcgtgtcga ggtgtgttga ctcctacagc aaagggcacg gatccgcggg 37260tcagcaagcc cgacgaattt acaccaatga cccgccgtgt cgccagtctc agaccagggc 37320acaacacgcc aaccctgcag accaccccca cctccccagc cactgcagac ccttccccct 37380ctgcttctgc agcacctgtc ccgaaacacg ctcattcccg cccccgcaga ccttccctac 37440ctcctggaga cccccctcaa cccctgtaga gtcccctgcc cgtgcagaat cccctccccc 37500ttcccccgca gcccccctac cccacccggc ggacagcccc gctcgcttcc tgttcttctc 37560ccaagggaga cccctgtcct ggctctgact ggggttgagt tcgccagttt tgacttatgt 37620gtagagtctt caccacgggc tggaagtacg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn 37680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 37740nnnnnnnnnn nngagcgtac ttcagcccgg gtgaagattc cttacacctt atacaaaaat 37800taattcaagt tggattaaag acttacatgt cagacctaaa accataaaat ccttagaaga 37860aaacctaggc aataccattc aggacatagg catgggcaag gacttcatgt ctgaaacacc 37920aaaagcaatg ggaacaaaag ccaaaataga caaatgggat ctaattaaac taaagagctt 37980ctgcacagca aaagaaacta ccatcagagt gaacaggcaa cccacagaat gggagaaaat 38040ttttgcacct actcatctga caaagggcta atatccagaa tctacaatga actcaaacaa 38100atttacaagc aaaaaacaaa caaccccatc aaaaagtagg caaaggatat gaacagacac 38160ttctcaaaag aagacattta tgcagccaaa aaacacatga aaaaatgctc atcatcactg 38220gccatcagag aaatgcaaat caaaaccaca gtgagatgcc atctcacacc agttagaatg 38280gcgatcatta aaaagtcagg aaacaacagg tgctggagag gatgtggaga aataggaaca 38340cttttacact gttggtggga ctgtaaacta gttcaaccat tgtggaagtc agtgtggcaa 38400ttcctcaagg atctagaact agaaatacca tttgacccag caatcccatt actgggtata 38460tacccaaagg actataaatc atgctgctat aaagacacat gcacacgtag gtttattgtg 38520gcactattcc caagagcaaa gacttggaac caacccaaat ttccaacaat gatagactgg 38580attaagaaaa tgtggcacat atacaccatg gaatactatg cagccataaa aaatgatgag 38640ttcatgtcct ttgtagggac atggatgaag ctggaaacta tcattctcag caaactattg 38700caaggacaaa aaaccaaaca cctcatgttc tcactcatag gtgggaattg aacaatgaga 38760acacatggac acaggaaggg gaacatcaca cactgggcac tgttgtgggg tggggggctg 38820ggggagggat agcattagga gatataccta atgctaaatg acgagttaat gggtgcagca 38880caccaacatg gcacatgtat acatatgtaa ctgacctgca cgttgtgcac atgtacccta 38940aaacttaaag tataataata aaaaaaaata aataaaaata aaaaataaaa gaaaatgaag 39000aaaatcatgt catttgcagc aacatggatt tggctagagg actttatcct aggcaaacaa 39060atacagaaac agaaaatcaa ataccacata ttctcactca taagggggag gtaaacactg 39120ggtactgatg gacataaaga tagcaacaat agacactggg gactattggg gtggtgaagg 39180agggaaggga gaaagtgttg aaaaactgtt gagtactatt ctcagtatct gggtaatggg 39240accattcata ccccaaacct caacatcaca caatataccc aggttacaaa cttgcacaag 39300tgccctctga atctaaaata aaagttgaaa aagcaaaaat aaaacagtaa acaacaacaa 39360caacaaaaca gcaaaaccct caataaatac ataaataaaa atataaataa atgtctttgc 39420cagagctttt tgttttttgt gtggctttga gttagtatct ggggtcacgt tgcttttaag 39480ctgtttgcta tcaactgcat cctatctaat atttcttttt cttaatagta aaccactcaa 39540tttttgtgag gagatattac attgtggttt tgatttacat ttctctaatg atttcctttc 39600cagtttggat gctgagcatt ttttcttatg ttttttgccc attcatatat aattttaaaa 39660gaaatgtcta ttcaaatcct tttgccattt taaaatcata ttatttttaa atattgtgtt 39720gtagaaatta tttatatatt ctggatatta actctttatc agatatataa tttactaata 39780ttttcttcca tccacaagtt ttttttaaca ttgttatgtc ctttaatgca taaaagtttt 39840aaaaagtttg atgtagtccc aattgtctat tttttctttt gttgtctgta cttttggtgt 39900catattaaaa aataattgcc atatcccctt attctaagag ttttatagtt ttagatcttc 39960catttaagtt ttaaatccat tttgagttaa ttcttgtata tggcataaga caaagtttca 40020acttcactct ttcatgtgtg gatatccaag tttcccaaca ctgtttgttg aagagaatgt 40080tcttccctca ttgagcggat ctggaatgct gtaaatatta tttgaccata tatgtggagt 40140tttcttttaa tgggttcttt attctattcc attggtctat atgtctctct ttaagccagt 40200gtcatgctgt tttaattact gtagtcttgt aacatgtttt gagatcacgg agcatgagac 40260cttcaactca gttagttttc aagattgtct agtcagagta tatatgaata tttagataaa 40320tttttctctt ttttcaaaaa atgcagctta gattttgata gggattacat ataatttgta 40380gactgctttg ggttctattg acttttcaaa ttaatacata atgttgtaca gatttgggga 40440atacatctga tattttggag atcttaacat taaatcttcc aatccatgaa taccaatgtc 40500tttacattta tttgtgactt ctttcagtac tgttttgtag ttttcagtgt acaagccttt 40560tatttccctg gctaagttta ttcctaagta ttttactatt tttgatggta ttgtaattaa 40620attattttct taattacctt tggattgttt atagttaatt tatagaaagg caactgattt 40680ttgtgtgtcg attttggatc ctgcaacttt ccagaatttg tttaatagtt ccaacagttt 40740tctggatttt atgcatatat gcatctaaca gttttctaga ttttatgcag gagtctagga 40800ttttatgcat ataggatcaa gtcatctgtg agcagagata attttacttt tttcttttat 40860tctttggatg tcttttcttg cttaattttt ttttcctaat tggtctgctt ggaaattcaa 40920atactatatt gaatagaagc gaagagacag agtatacttg tcttgttcct aatccagaag 40980aaaagtttga atcttttacc attgagtgta agtttacctg tggatttttc atatatggcc 41040tttattatgt tattttcctt ctattcctag tttgctgagt gttttcgtga taaaaggtgt 41100taaattttgt taaacatttt ttctacatca atataaatga tcatgctgtt ttgtccttta 41160ttctattact atagtgtatt acattgattt ttgcatgttg tattattctt tttttttgag 41220atgaaatctc attctgtcat tctggctgga gtgcagtggc atgacctcag ctcactgcaa 41280catttgcctc ctgggttcaa gggattctcc tgtctcagcc tcccaaagtg ctgggattac 41340aggcatgagc cagcacaccc tgccacatgt tctatcattc ttgcattcca ggaataaagc 41400caacctgatc atcacaaata ttggtctgta gttttctttt catgtaatgt ctttgtttga 41460ctttgaaatc agggtaatac tggcctcata taatgagctt tgaagtgttt tttatattca 41520tttttgtaaa tgactttgag aaacattggt tctaattctt ccttaaatgt ttggtagtca 41580tccggttctg ggctgttgtt tgtttggagg tttttgaacc agtttaatct ccttcgtagt 41640tataagtctg ttcgtttttt ctacttcttc atgattcact cttattaggt tgtatatttc 41700tagaaattta tgcacttcta aattatccaa tttgttgaca tataattaac tgttgatggt 41760attttcttat aatccttttg atttctatgg catgggttct aatgttctct tttttatttc 41820tgatttatta gttgaatctt ttttccttag ttaatctagc taagcatttg ttaattttgt 41880tggtgttttc aaaaaccacc tcttagtttt gttgattttt ttctattgtt tttatattct 41940atatttcatt tatctctgat ttaatctaat ctttaggatt ttcctccttc tgctagcttt 42000gggtgtagtt ttttttctct cctagtttcc tgaggtataa agttaggttg ttgatttgca 42060aactttcttc tacttctttt tttttttttc ttaagttgtg gagatggggt cttgctttgt 42120tgcccaggct ggtctcaaat tcctggcctc cagaagttct cctccctcag cctgtcaaag 42180tgctgggatt gcaggcatga cccaccatgc ctagccactt tcttcctttt ataatgcaca 42240tatttatagc tataaaattc tctgttagca ctgcttctgc tgcatcttac acattttggt 42300acattgtatt tttattttcc tttatctcaa gatattttct aacttccctt gtgatttctt 42360ttttgaccca ttgcttgttg aaaactgtgt tgtttaattt tcacatattt gtgaattttt 42420tgttttcctt ctgctactga tgtctagttt tatgtcattt tcatcaaaat agatattttg 42480tataatttca atcttttaaa atttgttaca agtttttttg tggtataaca tgtgatttat 42540ctttgggaat attccatgta aacttgagaa aaatactatt ctgattttat tgggtggaat 42600gttctgcaca tgtttgttag cactatttat agtgttgttc aagtactgta tttccttatc 42660gattttctgt cttgttgttc tagccattat tgtaagtgag gaattgaagt cgcctaccat 42720tattatgctg tttatgtttc ctctctgatc tgtcaatgtt tgcttcagat atttaggagc 42780tctggtatta gaggtatata tatttgtaat tgttatatct ttctgctgaa ttgacttttt 42840gttatataat atccattttt ttgtcttgtg actatttttg actttaagtc tattttgttg 42900gatagtagta tagtcacttc tgcttgcttt tatttaccat ttgcatggca tttacttttc 42960ctttttttca ttttaagcat atttgtttct tagacctaaa tgacaggata aagttggatc 43020ctttttacaa atccattttc cagtctatat cttttgattc aggagtttaa tccatttaca 43080tttaaagtaa ttactgatag ggaaggacct acacttgcca ttttgttgtt tcctgtatgt 43140tttgtaaatt ttgtttttta ttccttctct tactgacctc ttttgggttt tgtcgatttt 43200tttttgtggt gacattttga ttcccttcct gtttcctttt gtgtgtattc tatagatttt 43260tttatggtta ccataagaat tataaaaaac atcttaaagt gttaacaagc tattttaaac 43320tgttaagaga ataacttcga ctgtgtacaa aaactatttc ttcaaagctc ctaccctcac 43380cttaggttac ttatgtcaca agttacatct tcatatgtaa tgtattcatt aacacagttt 43440tatagttatt tttaagcttt tgtcttgtaa attctataca agaattaaaa gtgcatttat 43500gtaccaaaac tgcatagtac agtctctata tatgtccata tatttatctt tactggagag 43560ctttatattt tcataagact tcatgttact atttagcatc cttttgtttc aacttgcaag 43620atactacagc aggtccagtg gtaatgaact ccccagtttt tgttcatcct ggaaaggctg 43680atttttcctt tatgtttgaa ggatggtttt gctggatata atatttttgg ttgaaatttt 43740cttttctttc agctctttga atatattatc cccctgcctt ctggcctgca aggtttctgt 43800tgataaatcc actgataatt ttataaaggc ttccttttac atgacaagtt actttctttt 43860gctgctttca agattgtctc tttgtctttg acttttgaga gtttgattat agtgtgtctc 43920actgtgagtc tctgggtttt ctttaacatt ttttttttgt tcatatcttc tgatgatgaa 43980ttcctttaac ttttatattt gtgaaatgtt ttttcttcat tttcagaagg tatttttagt 44040gagtgtaaaa ttctacattg acagtttttt tctttcagta ctgtttagtg tttctctact 44100gtcttcttgc ttgtactgat tgtatagaaa ctctgctgta attcttatct ttgtgtcttt 44160gtacttaatg tgccttttta ctctgcctgt ttttaagatt ttatttttat tattgatttt 44220gatcatgtgg cttggtgtga gtttcttcat atttattgtg cttggggttc attgagctta 44280ttaagtctgt aggtttatat ttttcattac atttagaaaa attgtagcca ttattcattc 44340aaattttttt ctgttcttct cccatttctc atgcccttat ggggtctaat tatatgtata 44400ttaggctatt tgaggttgtc ttacagctca cttacagttc ttatgtttat ttttccactt 44460ctatttcatt tgggcaggtt tattatactt ttccctaatt tgctaatttt tttgcaatgt 44520ctaatccact gttaatctca tccaatgtat tattaatttc agacattgta gttttcatct 44580ctagaaattg ttcatctttt tatatcttct ttgtctctat ttgatatgct aagtcttttc 44640tctagcttct tgagcatatg aaatatagtt atgacaacta ttttatttta ttacaacttt 44700taccttaggt tcagaggtac atgtgcaggt ttgttatgta ggtaaactcg tgtcacagga 44760gtttgtttat tatttcatca ctcaggtact aagcctagta cccaatagtt gtttttctgt 44820tcctctccct tctcctaccc tccaacttca agtaggcccc agtgtttatt gttcctctct 44880ttgtatcact actattttaa tgtctctact agttctctta tttttgtcat ttctgagttg 44940attttatttt tctcactatg gccatattct cctacttctt tgaatatctg gtatgcaaat 45000tatacctagt atggatatta tatcaagacc ctgtcgcaat atggatacct aaaagaaaat 45060aacaatgttt accagtttgc tctaagcctt tcttagctct tttgcttgtt tctcaagaac 45120aagcacagag ggtggagctc aaccaagact gaagttaaag atgtagaaag attgagatgg 45180gaagtaatta agttgaggag ctctgataca atggtatttc tctttttaat catgtaagat 45240gcaagcccaa gataatatgt tgggaaagtt gaaggcgaat gtcttgagaa atatgaaatg 45300cacccaaggc tatggctggg tgatagaaat aactaaaaag ttttaaaagg attgttttat 45360agctggtcct tgctgaggtt aggggatagg cctggaaaaa ttgagcagtg gtattgccca 45420ggcaggactc ctcatgacat tctccagatc cctaagtttt tccaagtttc caagcatttc 45480cttggccagc aagtcatctc gcagcacaga tcctactctc tttcagtcag tcattccaca 45540actctacaaa gcatttttct ccatcaaatg ggaacttttg gggttctgca taatttttca 45600gggttccacc acttttgact ctttctggat accttgtaag gatattataa ctctttaatt 45660catgtccttg attttctctt ttaactttta atactgttca ttgcctgttt acctctttca 45720ccctcttctt ttcacttccg ccctctttct tgtgtaagta tataatcatc ctttttttct 45780tcctcgaatc tcagttctcg ggagtaacat atacaattca ttcttacttc cagtctttta 45840ttcctaagtt ccttatctct agttctcttg catatttgtg gcaattatta gtttttttcc 45900aaaaatccat tctccctttt tcctgtacat gtatctttcc agcttgacca catttcccag 45960cttccctggc agctaggtga agcaatgtaa ctaaactttt gcctgtaaaa tatgagcaga 46020agttatgtgc acaatatctg cctcacttgc ttaaaataaa ttgtattatt tgagctttct 46080atattgtccc tttttacaac taaaatgtag aggttatggc aacttttatg gtaaagagaa 46140tcatcacgta ggagatcaag aatcattgat ttgagaggaa cctaaatcct ttaatgactt 46200agtggagcaa gactgcccca tcagtctgga agctacccca caacttatgt atatcttcag 46260attgttgagc aaaagagaaa aaatgaagcc actatatatt taggtctctt catgttaaca 46320gtttagcttt taacctaaca cacatttatt cctgaatggt agcatattat taacatcaca 46380ggaagggaat tccttgttca tggatctttt attttgttga aataaaggta atctgcttgg 46440gcttaatact gccaaggcag aattttttgg aataattatt tctatagatg acccattcat 46500aatgacaact aacatggttt ctaaaatgga ttcatgtacg tcatcttatt tagtccccgc 46560aaaaattctg aaggtaggta taattattcc cattctatat attgaaaaat tgggattcag 46620agagactacg gacttataca agtttctgca gctatttaag aaacagagta aagtctcaaa 46680gccattaatg aactgtttag tttagtgctg cttagtaaaa ttagaactgt ttgaagtgcg 46740ttttaatagt aaacccatcg atatttagtc agctctctgc ttgctaacta atatggaaga 46800aaaaacatac atctacgtac atcctgaatt gggagaaggg aaataaaata ttgccaatcc 46860cctttcctgt attaggtact gtgataaggc gataaggctt ttcattatta taaatgagtc 46920ctggagaggt taagcgcagt cacccaaagt cacatgacca gtaactttca gagattttac 46980cccaggtctg gaaagactct gaagcccatg ttattgcctc cagagcctgg tggagaaccc 47040ctgaatggga cagagactca gcagacagag tggtcctttc cttccaggga gtcagggaag 47100gtttggtagg ggagatagag ttagatctgg gtgggatttg gatgaaatga gatcaagcag 47160ggataaataa gatccaagct gtttatgagg gacagggaga ggccagctct gttagaaaga 47220gtggtttata cagagaagca tggaaacatt tgctccatgc ctcgccttcc atggatagca 47280gtccagaaat gctacttgcc tgcctgacag tctttctcca gtccccaggt gtggacacgt 47340tctgagcacc tgagcctggg tctgccctga cccagattct gtgagctggg acagagaatg 47400tgtcctgctg tccctcctcc cccacttggg cccacaccct tttctacaca tttggattag 47460aaatctgaat tggcctttct gtgaacccca atgacaggca agtccccagt aaagaagcac 47520ttaatttaat tgctccttca gggtccactt agagtaatag ttgtgaacag ctctccctag 47580tgtctcccac aggcagtgac aaatgttgct aaagataagt cacaaattct tggcaaggcc 47640atcttaaata tgggaaggaa accttgttca gtcctgaaca gtcctgttca gactgatctt 47700atcacaggtt gcacactgcc tcaatgtcac catgtttcta ccctcagttg tgcttggcag 47760agagaggaca gctatacata aagtctgttc actctacaaa ggtgaaatca ttttttttaa 47820ggtaattatg attgttctga ggatggtgat tgagtgacat ttaaaaaatg ggaaaagttc 47880tactcataac tctgtctgct taatttgctt atataaaaga cttaatggtt tttgttgagt 47940tgatccagag tcagtaacat ttgaatatgt gttattacca tttgatcagt aacaaaacaa 48000cagggtaagt gggatgccaa atgtggctga gagtttaggg tttacattcc acaccccagc 48060cttaaatacg tttgtataaa ttattggttg atttttcttt ttcatggctt ttctttgaca 48120tttgatgggg ctggatttat ggaggcaacc aacaaattac acagcaatga attctggact 48180gtagtcccac agccttgagc ctagatacta gctgtgtcac taatactctg tgtgaccagg 48240gtaagttatt tagatacatt tatgactttc caaatcaata ggttggacaa agttatcttc 48300aaaatgtctt tcagatttga ccctgaacat atgcgattct ggcatgttct gatttttagt 48360tttctgttct tgaagcatgt ctacaataga aatgcctacc ttcatataca ataaaaggac 48420attagattaa tatggtgtct gtgaaaatca ttgaacagct ttagagataa aaataatgaa 48480atgagtcaag ttctccatta ttaattaaat cttttaaatt ttctgtttat gaaactatga 48540acaataaatt ggaacaaaga tataatctca ctacaagtaa attgtatttt atgtaaaata 48600taatttgtct ttagaaaata ctcaatgaaa ttaccttttt aatttcagag attttgagga 48660ctattattgt tatttcttta aagtgataaa tgtaatccaa gatgcctttt ttcccccttc 48720agaagcttga gtgcccaagg gtttgataaa tgtggaaaat aaccgctgtt cgaatatcct 48780gttggcctgc tggtgagatg tcatcgtgtt gtctgaatgc agtatgtgtt taaacttgcc 48840atttattacc tgaaattgtg ttattttgcc tcataccctt agtaatactc ttgtaatgct 48900ggctgagaat tgaagcaaat gccctatgaa actcaaggga gcaaggtctt tgttattttt 48960cagaagaaat gactggctga ggctgtaaag aaatcttgtg gtcattctca tggaaagagg 49020attggagccc cgattctttg atctcattat tgaccagttg attgagaaga ctgtcctcct 49080gtccccaccc aatcacaata agtgggtaat tacctgaggg ctaatttgct ccttgaatgt 49140gaatacattt gtggaaattt ccttgtgctc agcacctgtt caaaacatcc atttgtattg 49200cagagctatt gtttttctaa tctcattgtg gagctaagag agagattgtg tttaaggttt 49260ccttcgtaat aggttttctt gctccaggaa ggatgcgtga cagactctgc acactgctgc 49320ttggtaggtg ttcagtgatg gatgcgtttt ccatctccca ggtacagctg cagctgtgct 49380gactctggtg cgtttgtaat atgcactgtg gagtgcacca tgatttcatg atggctggag 49440aaatctggtg gctagaattc tagggaggag gacatgagtc ggaagtgcag aaacctcaca 49500ctgggacatt gatggagaaa atccaattct gtccctggag ttaaagagga gtcactcact 49560gggccatcat aagagcaaat aaagttacag ttatttcttc agatccacaa ctcaaactca 49620gcgaaagtaa ttattcactc tttttgtccc aaacaataat gaaatcagct aaagcatatt 49680gtatttatta cttccactca agttattgag ctgttctgac accttgttgc tgggaagata 49740cacagatgga acaccatcat ttccattgat tctgatcata ccccacgcta atatagcctt 49800attgctctga gtcataggga ttttatgctt gcctattcaa aaaacagttt ttaccttatt 49860aaaaggtgat atgacctgag ctggagatga tgtttggtca ttttgtgtaa accttttaat 49920attttagtgg taaatacatt tcttgaatct tgacaaaaac ataattaaaa tgaacatatt 49980taaaatggac atcttccaaa tgttcatttt taaaacaaac atcttccaaa tgttcatttt 50040taaaatgaat atcttcgtta actttttttt ttttttttga gacggagtct cgctctgtca 50100cccaggctgg agtgcagtgg cgcgatctcg gctcactgca agctccgcct cccgggttca 50160cgccattctc ctgcctcagc ctccagagta gctaggacta caggcgcccg ccaccacgcc 50220cggctaattt ttgtattttt agtagagacg gggtttcacc gtgttagcca ggaaggtctc 50280gatctcctga ccttctgatc caccctccac ggcctcccaa agtgctggga ttacaggcgt 50340gagtcaccac gcccagcctc ttccttaact tttaatgaat aaagagttga aagggaaaaa 50400ccaaatgtgc actatgctga ttttatcttg agctgtacag aatgtgcagt aacttaagaa 50460tataagtcat tctgctgtta ttcatactag cctattcttt agcattattt aaacacacat 50520atttgagtgt ctacacactg cctgctaggc agggggctgg gtgccaagaa gggatgtcca 50580gagataaacg ggcatatgtg ttaatctttt agggcacagt ccagtaacct gatcaaggag 50640tgacgtgtat tgtgtactag gatagaagtc agtatcagag gaaacacaaa ggaaagagtg 50700gtactttctc cccagggaag ggatgctcag aaagaggggg ctgattatgt ccctggttca 50760tgagaaaaag ggcattcctg gctcagggag tgggaagaac aaaaccaaga aatcctggaa 50820tggcttggtg catcagccag tctgtgagtg cagggcatgg ctggagttct ggctttaaga 50880tgagtgggaa gaaaggctgg agaggaggtg gatccaggaa aagttgctgg gagcatttat 50940tcttttggca atgggaagcc atggaagaac tgttgtaagg aggattctgt gtgatacatc 51000tgccttttag agaggtcatt ttatagaaaa agtagaaaat acagaccagc aaaaatttaa 51060aaagtgaaaa caatcactgt tcaagctctg gtctagcact caccagtgtt tttcctacag 51120gtgcatatac aaacatgctt ttacatttta tttttatttt aaatttttca ctatcctcaa 51180tatcagtgga aacaaatctg cttttttaat ttttttttta aaaatgagat cctattgtac 51240acattatttt aaattttttt tcattctttc ctttttttaa aattaaaaca attttttttg 51300agacagtctc actctgtcgc ccaggctaga gtgcagcatc gtggtctcag ctcaccgaaa 51360ccttgcctcc caggttcaag cgattctcct gcctcagcct tccaagtagc tgggattaca 51420gacatccacc accaccgtgt caggttaatt tttgtatttt tagtagagaa tggggtttca 51480ccattttgga caggctggtc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51600nnagcgtatt cgcccgtggt gaagatcgta gtagagtcta ccacctcggc cgtgaacctg 51660gctcaggggg cgcagccggg gtgggcgacc cagccaggtc tgcggagcgg tgcccggcgg 51720caaccccctc acggacgctg ggactccccc accccgtccc cattcagcgc tagcggtgga 51780ggggccaggc ccgccccagg tagtcaggca ggggtcccgg ctcctccccc aggaccaccg 51840ccccgcgggc gctcacgtcc tgatggcttc ccacaccagg agcccgtcgt cccggtagaa 51900gtagtagggg atgtcttctt tgctctccat gccccgggcc ttgatggcct cgggaaagca 51960cagggaggca taggtcaggt ccttcatggc cctctgcacc atctgcacgt gcccaccgcc 52020ccctgtggcg ttggcctgga gtgggaggta gagtgccctg agcgggtggc tcctcccaca 52080gctgtccaga cccatccctg aggaaatgtg ccaggggagg cgaggaggcc aggcaggcag 52140aggtgcaagc ccttgtcctg tgagatgggg gagggatgtt taacgcagag aatgacaagg 52200gcagccgggt ggagaggtgc tagggaggag cctcccgccc aatccaatgc aagggactga 52260accgtggggc tgcagagtac aagagggaag ggtgcgctcc cagccacagg agggcagcag 52320gcgtgggcag gtgtgtgggg gaccccagca ccagcgggcc tgggctgtga agtctgctct 52380agtgctgagc acacgaccct cagtcattgc tctcagtgac cctcagtttc caggacccat 52440gcaactctgt tgagggcctg ttgtgtgctc aggtgagggc tggagagacc cacaaggagg 52500gatcctggtc ctgatcccga tcccggcttc tgagtgatgc agacacactc acaagctcac 52560tggcacatat gtgtgtccac gtgcacacac acacatgcaa atacacccag ggggctgggc 52620tgtcggtgcc acagaggttt tgaagaagga cttgggtgct ggggtgccag agattggtcc 52680acggtgggca ttcagcccaa gcaggaccaa ccagagctgc cccccagcac tttctgttta 52740gatgcggctg ggagataatg gccacatgag aacctgaggt ggagctacta tgatgagctc 52800ctgtgccctc tcccatgggg gcctgtctgc agcaggagag tgtgatgcca acacaggtga 52860aagagacaga aagagggagc cagtgtgcat gcaccctggg gcattgcttg agtccctgga 52920tccatttctg gctgaggccc ccgaccctct attgtgtcca ggtatttgag ataaggcatg 52980tcttgtccag ctgcagctgg tttgagttgg gttttgtcat ttgcagccca aacagtgtct 53040caacttcagt ggtcatctgg cctggtcact aatgggctgg ggaaaggagg aaggttgggg 53100caagggccca tgtgagagtc caagcttggt aaggctacag agggctcaaa acgaggtggg 53160gtgctacctg agggatgcga gggctgggca gggcctggag tccccgatgc aggagacagt 53220gggttgggag ggttgggacc cgggaagcag agtgctgggg aggtccaggt cagggtggca 53280ggggtgtggc agattaagag gcagacacag gttcctggtg cgtaggaggt cagggggcca 53340taaggagtgt gttccagagg ggggtctaca cccaggtccc agaggctggg agaggggagg 53400ggtgggagga ggggaccagg agccatcagt gagaaggaag acctggtggt gcgcctggag 53460gagtgctcct ggctgaatgg cacgggctgg agacatcagg aggatgctgg agccagtgct 53520ctgagcagac tccagccttc acccttccag gcctcctgaa agggtgtcta gatctcagat 53580agggagtgaa ggcggccagg ctgggctctt ccaggcaaca agtagggtag gagggcaccc 53640accttgtcaa agaggccaca ctcgcagatg agctgctcac gggccttggt gttgattgca 53700atggtgaatc tcacgtgtgc caccagcagc tggggagggg agaggaggag gcctcagagg 53760gggcccagtc aggtgacctg gtgcctcagg gacccgcagc tcccccaccc aggcctgccc 53820tataggatga tgctgggcgc agggaggaag tgaaggctgg gtgctgatct ttctgctgaa 53880taagtgctgg aggcctgcac ccctctgatg cttactaagc atgataatga ttcagagggt 53940cctggccagc acttgtacaa tgcttacctc tttgctccct gagttcactg tcttaatctg 54000accatcattc tgtaaggcag ctgtactatt tgacctgttt tactggtgag gaaactgagg 54060cacatgggta cttttagcat gcagtcaccc cagcagccaa tgcttgtagg attcgagaac 54120agggacggag agtgatggca ggggcggcca tcagcatcag ccgaggatca ccatgaactc 54180atttgccttt tccaactcta ctggctggcc agcgaggccc gtgtgtcagg cccatgcccc 54240agccgcgtgt cctggggaac agcaggctcc tagctgtttt cttcctttag gggcattact 54300gactctgaga acatctctcc agggctccct ttctgccacc cactcccagc cacaggtccc 54360cctgtgggat gtcagtgatc cctcccactc cacatctcag atgccctccc atagcaggtg 54420gggcggtagc tggctgtacc ttgaaaatgg ggtgcacagc aggcagctgg cggtacattg 54480caatgccaaa aacctcagac accagatgtg ttcgcagaag gtgggtgatg gtctggtgga 54540cgtggaagtc actggaacgc acccagattt tggccaaaag ccagtcgtat tttgcatccg 54600aagggaggaa aatagggttc tcatctcccg ggatttggtt gagctgatac attgggggaa 54660aggaaacaca ctgctatatt tcacttcaga gtatagttgt tggagaactt cgcctcttgg 54720gaagttccag aagtatctga atttctttaa cagtgacttt ggaatcagaa gagcctggag 54780ggggtaaagg gccctgcggt ggtatttcct gtcccagcca tattacttac aacctgcatg 54840actctggatg acacactggg ctttctaagc tccggcttca gcatctgtca tgtggggaca 54900gtgatcgctc ggctcacagt tactcaggag ctggcagcat ggaaggagcg ggcaggccga 54960aggcccagca gcggaggggg aggatcgctg tccccctctt ccctccttgc cctgctccct 55020cttctactgc ctctccttgg tctctcatac tttcctccct ctcctcttcc ctgaaatgct 55080ggagctcccc agggtctgcc atggtgctcc tgtcttcttt tcttgcccta agtcagctcc 55140tcctgtttaa aggcttgggg ccctcctggt actgagcctc ccagatcagg gtctgcagct 55200tgagctctgc ctggagccca cacctgtggc caggcacctc ctgtagcaga ggctggacct 55260gtcttccctt tccagtttcc actccacact gggacaaggt gagttttttt cttttccaaa 55320atgcaaatca tgtcatgtta actgcctcct caatcacctc ttaatgccag aaaataaaat 55380ccccaagctc tgtggtgacc tggttgctga tgcctctgta cacacagcct cactttcgtg 55440cccccacacc agcacccagc cccaggtccc tccatgccag cagccctgca cgttgccccc 55500ttattgagag cccagcacag cctcagcacc cgacagtgag ttctcacggg agccggcagc 55560aggtgctggc attgcccatt tttacatgaa gacatggggt attggagggg ttgacagaaa 55620gaatgaggtg ggtggcaggg ctgcagccaa gagcggccag gaaggggcat ggccagactt 55680taggtgctgg gaaagaccag tgtcactgca gcacagtggt catggctggg gagggagaac 55740tgtcccagag cacacagctg tgtcacttgc tataagtatt gtggaaggtg gggtccttgg 55800gcgtctctcc ctcgaaaaca gtgctgtcca atagacatat aatgctaccc cacacatgga 55860attgtatata ttcaggaagc catattaata aaaataaaag gaaacaggtg aaatgccggt 55920gaataatata attttatttt acccaatata tccagaatat tatcacttca acatgaaacc 55980aatctaaaac tattaaatga gatatttcac attccccacc tccttattct aagtcttaaa 56040aatcaggtgt gaactttgca cttgcagcag gttttggttt ggacgagcca aatcccaagt 56100gctccagagc cctggtggcc agtggctcca ccctggtcag agagacccca gaagcctcag 56160cactccctga ggaccaagga ggggggaggg aggagggaga gcagagtgtg acttgcacat 56220taggcgtgcc tgtctgttta tacttgagat tagttggctg cttttaaata ccagacagtt 56280tcacataaaa atttgaatga cctggttttc ttgaaaaatt agaaggtctc agaactctgc 56340acccaggttc tcacagaact acgctcttgt cccttttaca gagtcccagc tctcagggtc 56400cccactgcca cacccgtttg catgagatgc ctgggccact gatacctgat ctgtgcccta 56460gaatgctggg ctgcctggca tgctctgaga cactttctga catgtcccca catgctctga 56520catgttcctg atggctctgg gacactgaca tgttcctgat ggctctggga cactgacatg 56580ttcctgacat cctctggggc cgaggagccc ccctgttgtg tgctaggggc tttacccatg 56640ctatctcact taatcctcac tttgaccctg caaggcagat attccttgct gtacccattt 56700tacatggggg gaaactgagg ctccagctgg tttagcagct ctcccaagtc acacagtagg 56760taaagagctc agccggcaac atgatattga acaggcaaaa attggaagca atccccctaa 56820aaactggaac aagacaagga tgtccactct cacccctcct attcaataga gtactggaag 56880tcctagtcag agcaattagg caagagaaag aaataaaagg gatccaaatg aaaaaaaaaa 56940aaagaggaag tcaaattatc tttgttcatc catgatatga ttctatacct agaaaaccct 57000gaagattcct ccaaaagact cctagacttg ataaacaact tcagtaaagt ctcaatgtta 57060taagtaaaat gcttgtttag aaacagaatg cttgttcctc ggtactgcaa ggaaaaatca 57120gcatttagac aaaaagtttt tttagcaagc caattttact ttctgcagaa agggtgctcc 57180tcgcagatgg aacaatggcg agagcacacc tgaataatgg agagaagcaa tttttattcc 57240ttacgcagct tgtccctgct accgtgtcct gtctccattg gctggagcca aacctcacag 57300tctaaactaa aacccgactg gctaataact taaaactttt ctaaataggt aaaagcagtg 57360gaaagacaaa ggaaaagagg aagttgctta tgaaaggact tagaaaagta ataacatttc 57420caaataagga aggggcatgg gctgcaagct gggacatgcc tgtgagcacg tccagcacaa 57480atatcttggt taaagtacaa ggacatagaa tgtactcatt cccttatatc taacagctac 57540atatgatagg gcttaacaaa aagttattag cacaaagcga aaaggcttaa aaaaagttag 57600tctttagaaa aaactattat ttctaacaca taatttactc cttaacaaaa aaaaaacttt 57660tgactttcta cactcaagat acaaaatcag tgtacaaaaa tcagtagcat ttctttttat 57720ttgagacgga gtctcactat cacccaggct ggagtgcagt ggtgtgatcg cagctcactg 57780caacctccgc ctcccaggtt caagcaattc tcctgcctca gcctcccaag tagctgggat 57840tacaggcagg tgttaccaca cctggctaat ttttgtattt ttaattgaga cggggtttca 57900ccatgttggt caggctggtc tcaaactcct gacctcaggt gatctgccca cctcggcctc 57960ccaaagtgct gggattacag gcgtgagcca ctgtgcctgg cctaaaaatc agtagcattt 58020ctatgcacca acaatgttca ggttcaggct gagaaccata tcaggaacgt ggtgatctca 58080gttacaatag tcccccatac acaaaataaa ataccaaaga atgtatccag ccaaggaggt 58140gaaagagctc tacagagaga actataaaac actgatgaaa gaaatcatag atgacacaaa 58200aaaatggaaa aacatcccag ctaatgggtt ggaagaaagc aatctacaga tacaacacaa 58260ttcctatcaa attatcaaca tcatttttca cagaattaga aaaaaaaatc ctaaaattca 58320tatggaacca aaaaatagcc gaatagccaa agcaatccta ggcaaaaata caaagctgga 58380ggcatcacat tacctgactt caaactatac tacaagacta cagtaacaaa aacagcatgg 58440tatactggta caaaaatagg cacagagatc aatggaacag aacagagaac ccagaaataa 58500agccatatac ctgcaaccaa ctgatttttg acaaaatcaa caaaaataaa caataaggaa 58560aggacatcct attcaataaa tagtgttgga aaaactggct acctatatgc agaagaatga 58620cgctgggccc ctacctctca ccatatacaa aaattacctc tagatggatt acagacttaa 58680atgtaagact tcaaacttta aacattctag aagaaaacct aggaaaaaat cttatggaca 58740tttcctaggc aaagaattta tgactagacc tcaaaggcat atgcaacaaa aacaaaaatt 58800gacaaatgag acttaatcaa attaaagagc ttctgcacag caaaacaaac tatcaacaaa 58860gtaaacagac aacctacaga atgagagaaa atatttgcaa ctatgcatcc acaaacgact 58920aatacccaga atctatgagg aacttaagaa aaaacaccac cattaaaaag tgggcaaagg 58980acaggaacag acacttctca aaagaagaca tacaagtggc caacaaacat atgaaaaaat 59040gcccaacatc actaatcatc agagaaatgc aaattaaaac cacaaaggga taccagtcag 59100aatggctatt attaaaaagt aaaaaaatga cagatgttgg caggatgcag acaaaaggga 59160atgcttacac actgttggtg gcagtgtaaa ttacttccat ccctgtggaa agcagtttgg 59220agattttgca aagaactgca aatagaatta ccagttgacc caacaacccc attactgtgt 59280acctacccaa aggaaaagaa attgttttac caaaaggaca taggcacaca tatgtttatt 59340gcagcactgt tcacaatatc aaaatcatgt aatcaaccta ggtgcccatc aatagtggca 59400ccagataaag aaaatgtgat acatgtactc caaggaatac tacatagcca taaaaaagaa 59460tgaaatcgtg ttctttgcag caacatggat gcagcttagg attggcactt aagccataat 59520cttaagtgaa ttaacccaga aacagaaaat cgaatactgc atgttcttat ttataagtgg 59580gagctaagca ttgtgtacac atgggcataa aagtgggaac aaaagacact ggggactcca 59640ggaaggggag ggagggagga aagggttgaa aaactgggta ttgggtacta tgctcagtat 59700ttgagtgatg ggttcaatag aagcctaacc ccagcattat gcaatataac catgtaacaa 59760acctgcacat gtacctgaat ctaaaatttt atatattaaa aaaaaaaaaa ctgttctgac 59820cagtaaaaaa agagctgaat gaggatgggc ccagtgctct gcctatttca gcccatcact 59880ctcccttctg cttctcttca cctcccggcc tgaccgggcc tcacctgccc atctccttga 59940gctcttgtac cactgagctg ggccttcccc aacccatcct tcacatcctc ccactcctgt 60000ccaggatttt ggcctgccat gggatgccag aacttgccct gagcaatttg tgtttcttaa 60060atttgttttt tgggtaagga agtagctttg cgtttctgat tagaaaagaa tatgtgctta 60120tcgtaaaaag aaatctgagt catgacagga gagcctgaga aagaaaacat catctcatct 60180tccagcgccc agggataacc accaaattct ggggagcctc ctttcatagg tctttgcatg 60240tgtgcacatt tatctgtcca attttccatc aactgaatat gattataatc aacatctgtc 60300tctttgtttt gctcacctgt cccccactgg gccatcactg tgttcctacc tggtgacact 60360gcgtccttgt gtccccatgc tggcacagta ggcctgctgc atccttgcat tcattttaca 60420taagtgagac catgcccatt gccttagcag tttgaaggag ctatttgtat acgacagata 60480tgtatccttt atcggtccct tgtgtcttgt ttttccactg ttaactcatt gtggagaccc 60540ctccaggtca gctggaaggg aaccaaagca ttcctttcaa agacgcctca acattttatg 60600gtgaggacat ggaagggctc actcactcaa tcctcttctg ccttcagact gttttcaatt 60660tttttcccca tcacaaacta tgctcctgtt gctacccaca cacatttgtg ctttcatttc 60720tgctgagtgg ctgctagcga aggctgagcc ttgggggttt gggtatgttt tcgagtagac 60780agtgcctgcc ctactgttcc tcagacagtt tcactctcct ggtgcacacc tgttcagtgc 60840tactggctgt cagaacactt aaacaataaa gtcccctcca gtgctcctcc aattgagacg 60900gggcaggggc ctcctcttag ggcactgtag ccccccagcc ccgcaacagg gcatggaaat 60960gaaggaaaat cccgagttcc ttgaagggaa gttccaggca cctagctagc cctgagaaat 61020aaatgagcaa cttgacaaac aagagggtaa cagcagccta aaacaacagc caaggaagcc 61080agagtcctgg gctgtttggt tccctctgga aactaaagag aacccctgaa catgtgtctc 61140tgagttgttt ttcagaaacc cagaccccca ccaaaccgat ccactggcac agagacctca 61200gataagacgg aactgaggac tgaactctga gcaggctctt tgttctaaat ttcttcctgg 61260ggggcctggg ggaagtcccg cccacaagcc acagctaaca ttcctttctg gtaacccaaa 61320tttttaaatg aaacttctct tccttaacca atttcaaaac aacaaatttt tgaatctacc 61380tatgacctgg aagcccaccc ccacttcaag atatcccgcc cttttaggcc aaaaccaatg 61440tgtaccctcc atgtattgat taatgatttt gcctgttaac ttctgcaatt cctgaaattc 61500actctgcctt taaaaaccct tacctgcaag ccatcaggga gttccggtct taagagtgag 61560ctgcccaatt ctcctttttt ggtccccgca ataaatgcct tgtgttctcg tagattatat 61620cccaatctca gtgtttggtt ttgctgcact gggcaagcag atcccagtcc agctcggtaa 61680cacaactgtc ctcgccacga cagcctcaac cccaggaccc tctgaccgcc cactgcaaca 61740tgcacctggt acagcaggcg ctgtgggatc tgctccggtg ctggccgaag ctctgacctt 61800gctccggtgc tggccgaagc tctgaccttg ggctgcccag gcccaccagt tatcccgtga 61860acactgccag gctgcaacaa ctcatgagca gagcatgggg ctccgtcctt gctggtgtct 61920gggagttacc cctttgtttt tccaaccagg catgtcagaa atgatatctc attgttaact 61980ttcatttgca tttctctaac tacaggtgaa gttaggagtt tttccatatg ctggttgccc 62040atttacattt cttcttttgt gaattgccta ttcatattct ctgctcattt ttccactgga 62100gtattggtat tttcccgtca atgtaaagga gctacctgtg tatcatagat aggcatctct 62160tagctgtcac ttctgttatg gttactcacc ctaatctctg ttttgctttt tggctttgtt 62220tatggtttat cactcaagtt ttaaaacatt acataatcaa atatgcttag ctttcccctt 62280ataacttctt ggtttccttt tccttctaaa agtcttctga gtgtctgcgt aattagtttt 62340tctaaattcg tttacttttt tcattttaat ttttacttca tctgaatttt ttctttttct 62400tctaagcaac tcctctacat gatgagattt atttttgtaa atggcatgac agagcagtcc 62460aatttaattt tcttctcatg caaaggcagt gtgatagcat cattaaaata atatcccttt 62520ttcactgagt tgaaaaacct ccattgtgat acactgaatt cctctgtggg ggagtccatt 62580tctggatctt actctatact gtcccccaat tctctctgtt ttcaagcaca gtgtctgctt 62640aacagtaacc ttacaaatcc agcaaggaaa gtgttccttc tgtgttttct tttacttttt 62700tttttttttt taaagatagg gtcttactct gttgcccagt gtggagtgca gtggagcgac 62760catggctcac tgcagcctca aactcctgga ctcaagccat cctcccacct cagcctcctg 62820agcagttggg attacaggca tgcgccacca tacctaattt tttctattaa tttttttttt 62880ttttaagaca tgaggtctca cttggttgcc ctgcctctct atgtggtttt aatttttttc 62940aaaaaatttt tgactatgct agatttcttt ttccatatga atctttcaat tttttaattg 63000ttttctctcc tttttagttc ctcttcacca ccaccacacc cccaccaaga aagataaaaa 63060attctggctg aaattttact tggaagtgca ttaagttttt ataatagaat ttggtagaac 63120tgacattttt acagtttcct ccccaatatc aaatcactgt taagagttca ttgtatatcc 63180tttaaaataa ctttatagtt ggccgggtgc ggtagctcac gcctgtaatg ccaacacttt 63240gggaggctga ggcaggcaaa tcacaaggtc aggagttcga gaccagcctg gccaacatgg 63300tgaaatgccg tctctactaa aaatacaaaa aattagctgg gcgtggtggc aggtgcctgt 63360aatcccagct actcgggagg ctgaggcagg agaattgctt gaacctggga ggtggaggtt 63420gcagtgagcc gagatcacgc cactgcactc tagcctgggt gacagtgcga aactccatct 63480caaataaata aataaataaa taaataaata aataaataaa taactttata gtttttcctt 63540atatgggcat tatattttat tgttagtatg gagaaaggct attgattttt ttgagaaata 63600tatttatcta aacactttgc caaatgaatt tattaatcta gtagctttta aaaaaattag 63660aatctcttgt gttttctagg tctaccatag cattaccaga aaagatcatc ttccctgttc 63720tttgacaata tgtttattga ttgttttcct gttttatcat gtggtatgac ctctaaaata 63780atgttgaata aaaacagtga tatcagcatt cttattattt taatggaaat ggcattagag 63840ttgcaccttt ttgatgttag ctatctttat catataaagt aaggttcctc ctattaattt 63900ttattagttg tggctattaa atgttattaa attccctttt agtgtgaatt gatataatca 63960taaactttat ccttatgggt ataataaatt atggtggtag ctctcttgat attgaaatat 64020ctggaataaa ccctgcttgg ttattacata tagtcattta atacactgct gcactcactt 64080cgctaatatc acctttagtt ttcttttaaa tctttattta atacttggat tggtcttcag 64140ccttcttttg aagcctgcct gtatccagtt tcagaactaa gcttacacca gctttatatg 64200atcaatttgg ggctgataaa gagccctgga aatgtcagct gtctagtggc tgggtgagac 64260tcagctgctc gctgtgtagg tctgtttcca atcactatat ggaaattggc ctattttcta 64320caatttcttc taatttatct tttcctaggt ttttatccat ttctctaatt tttcaaattt 64380ttgccatatt actctcattt aaaaaatatg taatcataat tactgttctt tttttttttt 64440aagagacggg gtcttgatat gttgctcagg ctagactgct gtggctattc acaggtgtga 64500tcatagcaca ctacagcctc aagtgagcct cctgcctcag cctctcaagt agctgggact 64560acagttgtgt accaccatgg tgcctggcta taattactct ttttatgatt ccttttttat 64620tccgaatctt gcattttttt tttctatctc tttttttcca tcagcctcat gaggaggtta 64680ttattattat tttttggacc tttcaaagaa ccagatcttg gatttactat cctctggctt 64740tcatttgctt gagtctttat agtttgcaaa gtttccattt tcagaagaag aaagagaccc 64800aggaacattc tgtggcttgc tcaaggtccc acagcctgct agcaagtggc tgaactggaa 64860tttaagctag gcccctgact ccaggctgct ccaagagatc tctgagccaa gcttttgaag 64920gcaccatcag tggtgcctgg gatctccggg gatctcccag caattttgtc ttcttttagg 64980tcacctgtcc ctttacctgc tgcttcagtt tcttaggcac agggcttctc tctggtaaca 65040gacaggaagc tcctctgagg ctacgtgccc ctgggagact gtggggaggc tggaatctag 65100agtcagatga cctggcttga atcctagctc tgtggctggc tgtgggcacc tgtgtctcag 65160ttttcttatc tgggtaaaat gatcaacatc aatagagccc actacctagg atggttgtgg 65220tgatgattac acaaataaag ataggttatc taaaacccca gtggtcaaat gtgcttcaga 65280atccagaatt tttaggattt tagcagtggg tatacttcat tatgtgccat accccctccc 65340acccccagtc tagggcaaca tactatttta caatcgaaca gagcagtatt tctgcagtga 65400tccacataca tatcacattg aatggaaaaa gcctctaaat aacctcacct attgcttcaa 65460agaaggtttg tttacaaaca atgaatttgc tgcacagttt tgaaatatgc agcagttttc 65520agatttttta gatttagaat cgagggtaag ggattgcgga ctgtgcaggt taacccatag 65580gatagccttg gcatgtggct ggtgcctgac ctatccagct gctattgtgt ccacagctcc 65640caaggtccag ttcatggaca ttatttgctg aatgctcatc tgccagtctc ccacggtgtc 65700ctctgcgcag ttcctcccta ctcccaggga cctatgccca gtccagtcct ggccttggcc 65760cctgctgtct gcacaccagc tcccagcctg gcagcaggct ggtgccagga acagggggag 65820acaaagccct gtgcccgcct gcctttcctc cagggtctcc ctcttcctcc tgcctcatct 65880ccggtggaca taagaactca catcatcatc aaaggtggca taccaggact ggcctggttc 65940ctgaccccag aacccatggt gcccctcagt ggtcttggtc tccagcttag tccacagctt 66000cacctgcctg gcccctggca gcaccttgac tgggggccct tggtcagggt gctggggtgg 66060agagtgcaga atatgatccc agggcaggtc ctccacagtg tgcctcgact ggccaggggc 66120ttctcaagtg tgccttgggg agtagaaccc tctccgcccc tatggacagc ccacctcagg 66180gcctgcagga agccctagtg tctttcaaga catagcttcc tcagctggag tgtgaatgga 66240gccacccagg agccctggat cgcagaggac tgagctgctc agaaaggtgt gccccccagc 66300agcctacctg gatggcaatg gggacaatct tgttggccag gttcttatac agcaagcaga 66360tgggagcggc caggaactgg agtgtgcagg ggtctgtttt gttggcatcg atgccatcca 66420gcagctcaaa gtccacgatg aaaatgttcc cttgctgcag gaggcagaga gatgtgtcca 66480aatcaggctc agcagacgga ccaaaaatca ggcctgggga aagagtgatg acctctgggc 66540acccctctcc ctttcctgcg actcagcgga gctcagaatg tgtacttctc tcctctgact 66600ccaaggctga ttgattcaca tggaaggtgt tcacaggttg tggcagttcg gggtaagatg 66660aaggaatggc agcagctcat tcggcatttg tggtgtctga aacctgcttg actggcacga 66720aagatgagag accgacccga gtgtcagttt actaagaaga aagaggttag gtgaaataga 66780tgtgacgagt taaatgccaa tcttcaccac ggggctgaag gtccgnnnnn nnnnnnnnnn 66840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66900nnnnnnnnnn nnnnnnnnnn nnnnnggggt cctcagggac tgggcctcag cccgccggtg 66960gttccaccct aggcttcccc aagtcggtca agagccggga gcagctgtcg gagtacctga 67020ccgtggtgat cttcaccgcc tccgcccagc acgccgcggt caacttcggc caggtaggca 67080gggccgggcc cgctgggcag ggctcccttc tcaaggccgc tgcctcctcc cccgccccgg 67140ttctgcacgc gtactgcacc ctcggacagc ctcggggcct ggcacgggac ttgcaggatg 67200gattctgccc gctcagccaa gggcgctggc cgcggggaaa gaggatggac ggactgcagg 67260gcccgctgga gttggggggc acggggagga cggggcccag ggggcagctg ggcagcaggg 67320cttcgggggt gcccacgctt gctggcggtc gtctccgcag tacgactggt gctcctggat 67380ccccaatgcg cccccaacca tgcgagcccc gccaccgact gccaagggcg tggtgaccat 67440tgagcagatc gtggacacgc tgcccgaccg cggccgctcc tgctggcatc tgggtgcagt 67500gtgggcgctg agccagttcc aggaaaacga ggtgaagctg ggcagggcgg ggcacagccc 67560caggtcaccc caggttaagc ggttcctcag cctcagggct ttgtgactcg ggccccaagg 67620ctcacttgga gcaaaggaat cctgacttcc aaggctggaa gggcccagaa ggctgcagcc 67680gccaccaggt cccccggcct cagcctggac agagctcagg gtgtgcaggg caggagagca 67740cacagcccag gctttgctca ctgtcaccag agggtcgtgt gtgacgcccc ctccccccag 67800ctattgacaa agttcttgca catgtgtttt accctggtac tccagaggga atgaccaaga 67860gtttcctggg ttccttccgg acacgtgcat ctcatttaac ctaacaactg aatccggtgt 67920gtcttcgtgg atgcacccgc tttggggtag ctcagtactg catagaatgg ccggaccagt 67980ccaacctgct ccagactgct gggtgtctga gttgttcccg gccaatgtgt tggtccctca 68040ctggacatcc ttgtttttgt ggctttgcac atgtgttcag tatgaccgca cacattccta 68100gcagacaaat tccttggtca aagaatattg atactgaatt gccctccaca aactaaattc 68160aaaatcccat tatcaatagc ctatgtttct tggacctttc cagctcagaa gctttgtgat 68220cttaatccaa tacagtattt ttttaaaatg atatctagct ctgccccgca gacatctgtg 68280tgagaatgca aataagcaca ggaccccaac agggcagcca ccccttcagg ctccctggcc 68340cgctttctgc cttcctgggc tggagaggcc agtgctggcc ccagcgcccc tgatgggagg 68400tgagagtgct gcgcaggggt ggcccaagac agcaggatac catggctgca aacaccagca 68460gcccccagct tcatctgcat catctcgtaa ccacttggca gcaggcagtt attttcccac 68520ttatccatga agccccagcc ctggagcctt ccttagagaa gcaggttgga ggcgacgaca 68580cttgccttcc cgaggccctc ttgtcaggca gcagagggtg aatatgggga ggtgaataga 68640tgctccctcc ttcatctccc aaacggtggc tggccccttg ggatgagaca ggcctgtcag 68700tttacacggg tagtggattg acctatgtgt gtgtccatgt ctgggccctc agctgttcct 68760gggcatgtac ccagaagagc attttatcga gaagcctgtg aaggaagcca tggcccgatt 68820ccgcaagaac ctcgaggcca ttgtcagcgt gattgctgag cgcaacaaga agaagcagct 68880gccatattac tacttgtccc cagaccggat tccgaacagt gtggccatct gagcacactg 68940ccagtctcac tgtgggaagg ccagctgccc cagccagatg gactccagcc tgcctggcag 69000gctgtctggc caggcctctt ggcagtcaca tctcttcctc cgaggccagt acctttccat 69060ttattctttg atcttcaggg aactgcatag attgatcaaa gtgtaaacac catagggacc 69120cattctacac agagcaggac tgcacagcgt cctgtccaca cccagctcag catttccaca 69180ccaagcagca acagcaaatc acgaccactg atagatgtct attcttgttg gagacatggg 69240atgattattt tctgttctat ttgtgcttag tccaattcct tgcacatagt aggtacccaa 69300ttcaattact attgaatgaa ttaagaattg gttgccataa aaataaatca gttcatttaa 69360aatgggtctt gttccatgtg ttatattcca atcaccccaa acagctcact gccatctccc 69420acaccaagag aaaaaaaaag ccatagtcac cgtgatttta tcaaggcaaa agcctcccca 69480cccaagtctg gatagaaggt gctttttccc tcaagcagtg tctactctct ttggaacaca 69540tcccaaatgt caccacctct gggaaggcat ccttggttcc tgtctctacc ctggatttgt 69600cagaacctct gttcttgccc cctctgtggt ctctcccttg ggacaggagc ccctggaagg 69660acagaaaacc cactttattt atgtctgtgc cccagggctg gcataaggca ccagcactca 69720gctgttctct ccttttcctc ctggaagggc acctagctaa tggatattag gcattgtctt 69780gggagctggg ggcttatttt ttcctgaggc agggttctta acatgacata aaaaataaaa 69840atgggcaggg cgcggtggct cacgcctgta atcccagcat tttgggaggc caaggtggga 69900ggatcatttg aggtcagaat tcaagaccag cctggccaac acggtgaaac cccatctcta 69960ctgaaaatac aaaaattagc caggcatggt agcaggtacc tgtaatccca gctactcagg 70020aggctgaggc aggaaaattg catgaacctg ggagacagag gttgcagaga gtggatatcg 70080caccactgca ctccagcctg ggcaatagag cgagactctg tctcaaatag ttaataaata 70140aataaattaa attaaataaa aacgcattct ctttccccag ccccaagtag cagagcccgc 70200tctgagcctg ggcatggtga cccattctcc tcgtgctcac atgcccaact cccagccctc 70260cagcccctgg cttcggaatc atgtcatcag ggtctcccat gtctctggaa ggtgcttcca 70320gtgtggcagg ccatcagctc agtgtctggg taaaggcttc tacagggcca gcatggcata 70380ggggggaggt gtgcatgcgt tcctgaagcc acaaggtctt ctgaggagac ctgactattg 70440gggtggggac tagagactga ggtccagccc cttaatgccc cagactccct gccatccctc 70500ggagaatttc ccaccacact tatgaatgat cttcgggaca ggtgacataa acatacagct 70560atggtagagg cattcagctc catgccactg tgagaatgaa cataccaact tttaggcaac 70620aaggccatgt ggtcaaggag agaggagaaa cgaataggag taggcagggg tggtgaagca 70680ggtctgacat tggcaaaagt gacagagaag gattgggttg gaagggcttc agatggcaga 70740gaacctctga aaaagtctca tgccagctaa tgaagagtcc tcaaagtcac catcacagaa 70800atcttacatc tggcagaaat gacagcaata gtttggcatg aaccccacag tggatccaaa 70860cgtgtggcag ctgagatcgt cagttaatta tgttctctgc agctggtcct tctggttccc 70920tggagctggg tatctgagta gcacagctcc actgctacca caggagaggg agaatcccaa 70980attctgtgca tagactgtct aaacctctgg atgaccccta agacacatgt acataaggca 71040aactgcaaac agcccaattt aggataaaac agcctgtctt actctgtttg tgctgctata 71100ataaaatacc tcagactggg tcatttatat agaacagaaa ttgattcctc acagttctgg 71160aggctggaag tctaagagga ggcactggca gtttcagtgt ctggtaaggg agggatcagt 71220gtctgctccc aagaggatgc cctctgtgct gtgtcctcac ccagcagaag agcaaaaaag 71280ggcgaacatt ctcaaaagcc tcttttataa ggacatcaat ccattcatga gggcagaacc 71340ctcaagaatt aatcatttct caaaaggccc ctcctcctaa tgccatcacc ttggggttta 71400agttccaaca catgattttt ggaaggacac atacattcaa atcattgcac aaccaaagtg 71460atatttgaac tttcaaatag tttgcagttt gagatcaact aagttaattg cttgcttgaa 71520taaaaacatc acagccgggc acgatggctc acgcctgtaa ttccagcact ttgggaagcc 71580aaggtgggca gatcacttga ggtcaggagt tcgaaaccag cctgaccaac atggtgaaac 71640cccatttcta ctaaaaatac aaaaattagc caggcggccg ggcgtggtgg ctcatgcctg 71700taatcccagc tctttgggag gccgaggcgg gtggatcaca aggtcaggag attgagacca 71760tcctggccaa cacagtgaaa ccctgtctct actaaaaatg caaaaattag ctgttgagac 71820tactcgggaa gctgaggcag gagaattgct tgagccgaga tcgtgccatc gcactccagc 71880ctgggggaca agagcaagac ttcgtctcaa aaaaaaaaaa agaaaagaaa ttttctggat 71940taggataatg ttctatattt ggataagggt ttaggttacc tagatagata catttatcaa 72000aagtcatcaa atggtacact tcagatgtac gcatgtcact gtatgtaaat attaccccaa 72060aatcaacaag aaccatgagc aaatactgac ttctagtgaa cgacatgcat actgaaactt 72120tcgtagtgaa gtataattat ggctgcagtt gactctgaaa tacataaaaa cctaagttga 72180tgggtgaata cagtgattaa tagatagaaa tctgtggtaa agcatacgga gaaaaatgtt 72240aattgtagat tctagggttt atatatattc tcactatata attaaacgtt tttatgttta 72300aaaattttta taatgttgaa aaaccatgaa atcctaccta acgcagtaag acaagaaaaa 72360gagataaaag gtatatgtac tgggaagtaa gaaataaaac cgtctttgtt agcagatgac 72420atgctcatct atgtagaaaa tccaaaagaa tggataaaga aactcctgga actaataagt 72480ggttacagta aggttgtagg atacaaggtt aatatacaaa agtcaatcac tctcttattc 72540accagtaatg aacaagtgga aattgaaatt caaaacaaaa tactatttac attaatactc 72600caaaaataca tacttaggtt taaatctaat aaaatacata caagatcttt atgaggaaaa 72660ctgcaaccct gatgacagaa atcaaagaga aactaaagaa atagacataa gtaaatattc 72720cccagaaaga ctcaatattg tcaagatgtc agttcttcaa aacttgacct atagattcaa 72780tgcaatccta atcaaaatct cagcaagtta tgttgtggat actgacaaac ttattctttt 72840tttttttttt tttttttttg agacggagtt tcgctctgtt gcccaggctg gagtgcagtg 72900gcgcgatctc gactcactgc aagctccgcc tcccgggttc acgccattct cctgcctcag 72960cctcccatgt agctgggact acaggcacgc gccaccatgc ccggctaatt tttgtatttt 73020tagtagagac ggggtttcac cgtgttagcc aggatggtct cgatctcctg acctcgtgat 73080ccacccgtct cggcctccca aagtgctggg attacaggcg tgagccaccg cgcccggcca 73140acttattcta aaatttagat agggaagcaa aagacccaga acagctaaca caacaatata 73200gtaggaaaac aacaaatttg gaaaatggat actacccaac tccaagactt actataaagc 73260tacagtaatc aagatagtgc cgtattggtg aaagaataga caaatagatc aatggaacag 73320aatagagagc ctaagaaata gaccttcata aatacagtca actcatcctt gacaaagtag 73380catagacact acaatggagc aaagattgtc ttttcaacaa gtggtgcagg aacaactgga 73440tatccacatg caaaaacaaa caaacaagca tgaatctaga tacagacctt atgctcttca 73500caaaaattaa ctcaaaatgg attatagatc taaatgtaaa attcaaaact ataaaattcc 73560cataacatag gataaaatct agatgaccta ggtatggcta tgacttttta gacataacac 73620caaagccatg gtccatgaaa gaaataattg gtaagttgga cttaattaaa attaaaacct 73680tccgctctgc aaaagaccac actaggagag ggaggaaata tttacaaaga cacatctgat 73740aaagaattgt tacttaaatt atacaaagcc ttcttaaact taacagtaag gaaacaatct 73800gattgaaaaa tgggccaaag actttaacaa acaaaagaag atatacaaat ggcaagtaag 73860tatgtgaaaa gaagctgcac agctgggggg cgtggtggct cacgccttta atcccagcac 73920tttgggaggt caaggcgggc agatcacctg aggtcaagag accagcctgt ccaacatgga 73980aaaacctgtc tctactaaaa atataaaatt agctaggcat ggtgatgcat gcctgtcatc 74040ccagctactt gggaagctga ggcaggagaa tcgcttgaac ccggaaggtg gaggttgcag 74100tgagccaaga tcgcgccatt gcactccagc ctgggcaaca agagtaaaac tctgtctcaa 74160gaaaaaaaaa aaaaagctgc acattctatg ttatcaggga aatgcaaatt tagaataacg 74220caataacaag ataccactac atacatatta gaatggccaa aattgagaac actgacagca 74280ccaaatgtgg aggaggatgt ggagcaacag gaactagaga gaatgcaaaa tggtatagcc 74340acttaggaag acagcttggc agtttcttac aaaactaaat atactcttac cacacaatcc 74400agcaatcaca ctccttggta tttacttaca tcccaacaaa aaccctcata tggatgtcta 74460gagcagcttt gttcataatt gtcaaaactt ggaagcaacc aaagatgccc ttcggtaggt 74520gagtaaataa ataatatcca gacaatggaa ataccattca gtactaaaga gaaatgagct 74580atcaagccat gaaaaggcgt ggaagaaact gaagtgtgta tcactaaaag tcaatctgaa 74640aagggtgcat aagtttccaa agatatgata ttgcagaaaa ggcaaaactg tggagacagt 74700aaaaagattg ccaggggcta gggcagtggg agggatgaat aggcagggca cagaggattt 74760tcagggcaat aacactactc tgtatgacac tataatgttg gatacatggc cagatccaca 74820gaatgtagaa caccaagagt gaaccttgaa ctatggactt tgagtgatga ggatgagtca 74880gtgtaggctc atggattgta ataaatgcac cactgtgggg ggggggggat attgataact 74940ggggggctgt acatgtgtag ggacaggagt aaatgggaaa tctctacttt tgttaccggt 75000ggagggtgta ccgcattttg aacaaaaaat tggacaaaac gcacaaagca aggaaagaat 75060gaagcaacaa aagcagagat ttattgaaaa cgaaagtaca ctccacaggg tgggagcagg 75120cctaagcagg tggctcaagg gcctgaatac agatttttct ggggtttaaa taccctctag 75180aggtttccat tggttacttg gtgtataccc tacgcaaatg aagaggatga agggaggtta 75240caaagttatt tacttggtgt agaaaattag ggtttttccc ttttatttag ttgtaggaag 75300cccttaggtt acctgcctcc agaccctatt ctcctgcctc attttattcc taattttgtt 75360gtgaacctaa aattgctcta aaaaatgaaa tcttttaaac aagaaaataa acttataaat 75420tgcaaatatg tgtcacttac ataaaaacaa agaattagtg tccctacatc aggaaaaaaa 75480cacctataat tcaatgtaaa aatgcaaaaa agccaagaga aatggccaag ccatggaaat 75540tcacagaagt agaaataggc agtaaataaa caatgctcaa tttcatttat ctgagaaatg 75600gagagtaaaa cttcagtgac atacaaagga gtaacaatca ttttaggaac aattttaaaa 75660ataacacttg tgatgtggct caggaaagat tcatattctc actgtaggaa ggtaagtgaa 75720tcattttaga ggtcagtttt gcaacttaga agatcaaatg catctaccct ttttctactt 75780ctagaaatgt atcttcagaa gtgcccctcc ctccctcaca cacccccaag tgtacaaaaa 75840taaatgacat ggatgtggac tgcagccttg tttctaatac caaaaatcag taaacaacct 75900aaaaatctgt atccatgagg atttaaagaa atcctgcatg agttagaaag aataaggtag 75960atgcctgggt tctaacatag aaaatatcaa actggttgtt agatgaaaaa agcaagttgc 76020aaagtgaggt aatgtgtaaa tgcagtaagc aaaatacatc atatatatag tactttctat 76080gtatttacct atatgagcgt gatgcctcca actgcatagc tgtggctccg ggagcaggac 76140cttgtaacag agcctctgcc cctgactgtc ctgaggggcc tttccctggg aaaggaacca 76200ggtttgattt tcagtctctt ttgttatcac catgctgaca gctttgcttg tcatatgcct 76260ttccctccgc tgtactgatt ttaccttaca gaagctctcc ttagtgcttc atcagacact 76320ccctcttttc caaagaaaag acagccgcat tccatggtca tctggttgag ctcgaggtga 76380gccccaggga tcatgtgcct cccaccaggc tgacacccca gtggtgagga gttgcccctt 76440ggctttttcg aggccaagca actcagaggt gtggcaggat tgcatgtcac agtggcactt 76500ccaaggccct ggagagtgac tcaacgcctc tgttattaag atgtcacaca ggctgtgcag 76560caacaactct agggacaccc ttcacactga cagagttggc aagcttttcc tataaagggg 76620cacacagtga atactgtagg ctttgtaggc catactatct gtcccaacaa ttcaactttg 76680cccactgtag ctgtgaatca accgtagata atattaagtg aatgtgcact gctgtttgct 76740aatagtttat tgacaaaaac agctgctttt gagcagtttg ctaccccgac agagactgtg 76800gggagtccct ggtggcagcg agctacaggc agccagtacc ccatcagtgt ggctggttcc 76860atcctctcaa gcctgcctgc acccagacac tgtcgagtgt gtcttcagaa aaactcctaa 76920aattccccaa aagcacagtg gctccccgag agccacctcc ctcaccttct tacaggggca 76980ggttcctctt ccggttccac cctcctcagc acggagctca ggggccatcc ccacctcctc 77040ttggccgcaa gcacagacct tcccacacag gctcagcagc tctgcgggcc tgtcccccac 77100ctggagctgc aggcctccac tcctggggac agctgtgacc atggctctgc aaagcagcct 77160tgtgcctagc tcccaagtca gctggcagcc cagagtggaa caattctgtg gatcacctct 77220gagacagcag cacatctggg caagcctggc ttcccacccc tcacctcctg caacctctga 77280cccttgccat gcatgtggta gggaagctgc aaccaggcct cagcactccc agacctgtgg 77340gcggtttgct gtctgaggat cgtggcactg ccttccctca gaggccttgc cagagccagg 77400ggatgcagcg tggcagagct gtcactggca accctgctgg tctccctggt gctttctcag 77460ctgggctgtg ggtcaggaca aatttctatg gataaggctg gatcataatc tagtgctcca 77520tggtcatgca gcgacagact cgagtctgcc ttctctcagg gtggtgagct caggtaggaa 77580cgggatgttt gaagcaagca cacctgtgag aaatgcctgt tttcactccc atcccacaca 77640ctcgtccaga cactgccagc ccacccccga cacacgtgtc tgagggaggc tgaggaaaac 77700agacacctcc aggagccgca tactatttcc tgatgtgtaa gccagacaac cctggaaagg 77760aggtcaggga accggagtgc ctgccaacgt ccccagcctc cctgcagcaa aacactctca 77820tggcaaaact cctttttaaa gaaaggcttt attgctgcaa ttagacatcc cagtgtatag 77880cacaaacccc cctgtacaga gaattattca agtggtaata ctgagaaaca gtgaacacac 77940acaaaagaat acaaaactag acatttagat cactagaaac tttctaaggt aagaaaaatt 78000tcaaatgtga agtgcctttt agaaactaca ccacacatgc cagtgtaaat ttggtttaac 78060aatcaatttc tataaacatt gcatctaact gtgcactgat caactgcaca acttcaacaa 78120ttacttccaa gacaaagata aatgctttta tttccctatg ttttggtctt tccttgggct 78180agagacagaa gctgcatgtt tcaaaccaga ttcaaaaggg aaagcacttc aaaacagaaa 78240gtacttcaat tgtgtatttg ccttcagcca catccagaga cttgtggatc tcacccggtt 78300ggtcccagcc cttctccaac cctgaagccc acagcagggg tgcagaggca gccccagatc 78360gtgcagacat cacccgaggc tcccaaactg gcccagctgc ctgaggcctg catgcctgtg 78420gccaggtagg acacatgtgc aggtttgaaa actaggccta caagactgaa gagtggactg 78480aagcaaaaag gggagaaaca ttagaggcct acagtaggaa gagacctttt atagagtttt 78540caggaaattc tcacaataaa cttgggcaca aagaccaaat tgctgaggcc agaagcctgc 78600ttatggtact gtgacttatg gggagtagag gccaaatcag agagggctgg ctggtgatac 78660cccagaccac ttgcctcttc cccttccctg gcctacaaat accaggcctc ctggccctga 78720gacctcattc tgggtttcta tatgggtcaa ccaaatgcca tcctttcact aaacccaggt 78780gcaaaatggg gggggaaggg gggaagctgg ggagtacaca gcacccaaaa gaaccgagag 78840agcaagtttt ccaaggctac aggaacattg gcagagctgg tggccacacc agatgggagt 78900accacatccc caccaggctt catgcttcac tgctagatgc agggggctag aagggtctga 78960tgaatcagga gctgaactag aaccttcaag aaaagaaaag gcaagggggt ggctctgtgc 79020cccgccagga gaggaaagaa gaatgtgtgt ggattgtcca cagtgaggcc agaaacgatg 79080agatggtgtt aggcagaaaa gaagggagag ggcttcccat gagaatctgg ctgggcatgg 79140gagggataca gggctcagtt atcaaggcac ctttgccata gtggggtgcc agctacattg 79200ggtgggatgc ccaggggctg tccctaccta agcactttct tgtctacagg aagtcttggt 79260gcttttcaag ttcagcataa ggggttccat atgtgaagtg gggattcaca cttagaaaat 79320ctatcctggt cttcacctga aacactcatc tactgctggg tggcaggaac aagtatacaa 79380aaccaggggc cttgcctggg cagttcctaa gggacattgg cagggctgac ctgggattca 79440gctcccctga gtggacagtc tgtggttgcc tggggtcccc aggctgagaa ggggtatggg 79500taattgcact tcggggcaca gtgaacacag gcacgggctg gatgtgccct gggagacaga 79560aaggagaggc tgacctattc tcaggagctg gctatggctg gacagtgcct gcccggccag 79620aggaaggcca cagcagagcc actccacaga ctaagacctg tgttgccaag gaatcagaca 79680ctggcttgga ggagacagga ggacaaagcc tgctgctatt aagcccacac cgtagcttag 79740agatccttca gcctcctggc cttcaaaaca aactccaggg cttccactct gaaaggcaaa 79800ggaatcacct cgtgtttgct tcatcccctt agcaggatga acgaaacgcc taaggcccaa 79860aaagactgag ggcaggaccc ccagcatctc ccatctcctc cgtgcaggct gggtgagaga 79920atggcctgga aaggaagcat ctgcgctaca ttgtgcttca ggccccccca gcaactttcc 79980cggaggtaac tatttgcctg gaaaactagg aaactgggtt ttaaaaacag aattttaagc 80040agacttttta gagggacaag gccatttgat ggtagtgatt ttggttttgt tttatgaaaa 80100tggtacaggt ggtaatccct gatgacagaa tgcactgaga gtggggccaa gggccctgaa 80160ggatcctgtc tgttcagtct ggttaaggcg acagctgggc gggcatgtgg ccggggagca 80220gtggcacagc ggaccctggc agtttgctca cacctggccc tcgtcccaga gccagcccac 80280ctgcctcttg gccctggtag tgactccaca gaacttcccc gagcagaagc ctcacagtgg 80340gccagcaccc atcttgcatg caagggagaa cctgcatgca gtgcacacac cccggccagg 80400gtggcacatg gctgctcttt caatgtgaag ccaacaccaa ctggagagat ttaactataa 80460aggaatattt tttaaatatt cagttttgtt gtcattactc acttcctttc cttccatttc 80520gatctcagaa actgttcttt ttcagtgttg acaaaaaaga aatgtatggg ccaggtctac 80580aaagtaacac cttgtcataa aacaggctca aaagcatagg aatcatgctt taattgccat 80640ttagcatcca ctaattccca tgagatctgt gattaaaaaa aaagcctctg gcctgcttgt 80700cctgtcttaa aagtcatgag tctgttttat gaacagaaag aatgccacgc ttcttagcat 80760atgtaaggca aaatggattt ttgatctctc atgatgtaaa ggagatacaa aggtaaggaa 80820gggaagattt ctaagtgaga ctttttttga gtgtcaatcc cgctggacac acatattaca 80880aaataaagat tttcttctgt aaagtgctgt ttgctcagtg aatcatgcgc caccacagag 80940caccatggct ccacctgctc aagagcatgg aggaggcagc atcggcagag ggcaggcagg 81000agtctgtgtt tgggggtctg tttcaatacc atctcctggg gttcgccgtg atgcagaggg 81060aatcttctcg tcatggctag acactcccca atgctctgct ccaggctggg gaccactgca 81120agctggaggc ggctgggtat cagggcctgg gcacccctgc tcctcctctt cccttgggat 81180tggcatttta ttctctcatt cagctcaaga ccatgggcag gaactctgct ggctccccat 81240gatgtcatca tggggtcttc cactttccca cagagctgcc aggcagaggc aggacccagg 81300catgcctggc ccacaggaag gttttctagg agactaagga gggtttagaa aaagagaagc 81360cactataaat agtcacctgt ccagtctatg ctattaaagg acataagaaa ggtatacaat 81420tggcagtaaa caatttcctt cagctcttca tggatgtcca ggaaaatgac aacccgcaca 81480caatcagacg tgaatgattt ctgctccagt gtcttcaggc tctgtgcaac aagaagagtt 81540tgtgtcggaa tgacagattc catatccatg tttattttca aagttgggct ctgttagtgg 81600agatttttca aaaatattct ttttgcttgt ttctggacag ttttgaacat agatcactct 81660attataggcc ttgagtctct tccacaattg cacatacact ttacactgaa catacataaa 81720aagaagtcct ccggtgaagc cgatggccac aaccaccaat ttagtccaaa agggccattc 81780taggattcct ggaagggaaa aactcagcct attagatttt atttaagata tgtcttcacc 81840acggggctga agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 81900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcggtga 81960ctgtgacatt gaggttcggc gtcgggggag agggaaaagg agagggagag ggagagggag 82020agggagaggg agagggagag ggagagccat atgtagttta aagtagtttt tttccaattc 82080tgagaagaag gtcattggta gcttgatggg gatggcaatg actctataaa ttaccttggg 82140cagtatggct attttcacga tattgattct tcctacccat gagcatggaa tgttcttcca 82200tttgtttgta tcctctttta tttccttgag cagtggttta tatttctcct tgaagaggtc 82260cttcacatcc cttgtaagtt ggattcctag gtattttatt ctctttgaag caattgtgaa 82320tgggagttca ctcatgattt ggctctctgt ttgtctgtta ttggtgtata agaatgcttg 82380tgatttttac acattgattt tgtatactga gactctgctg aagttgccta tcagcttaag 82440gagattttgg gctgagacga tggggttttc taggtataca atcatgtcat ctgcagacag 82500ggacaatttg acttcctctt ttcctaatcg aatgcccttt atttccttct cttgcctgat 82560tgccctggct agaacttcca acactatgtt gaataggagt ggtgagagag ggcatccctg 82620tcttgtgcca gttttcaaag ggaatgcttc cagtttttgc ccatgatgta tgatattggc 82680tgtgggtttg tcatagatag ctcttattat tttgagatac atctcatcaa tacctaattt 82740attgagagtt tttagcatga agcgttgctg aattttgtca aaggcctttt ctgcatctat 82800tgaaataatc atgtggtttt tgtcattggt tctgtttgta tactgggtta catttattga 82860tttgtgtacg ttgaaccagc ccttgcatcc cagggatgaa gcccacttga tcatggtgga 82920taagcttttt gatgtgctgc tggattcgtt tgccagtatt tattgaggat ttttgcatcg 82980atgttcatca aggatattgg tctaaaatgg gttttttgtt gggtctctgc ccagctttgg 83040tctcaggatg atgcctcccc tattaaaaga gggagggagg attccctctt tttctattga 83100ttggaatagt ttcagaagga atggtaccag ctcctccttg tacctctggt agaattcggc 83160tgtgaatcct tcaggtcctg gactgttttt ggttggtaag gtattaattg ttgcctcaat 83220ttcagagcct gttattggtc tattcagaga ttcatcttct tcctggttta gtcttgggag 83280ggtgtatgta tggaggaatt tttccatttc ttctagattt tctagtttat ttgcatagag 83340gtgtttataa tattctctga tggtagttta tatttctgtg ggatcagtgg cgatatcccc 83400tttatcattt tttattgcat ctatttgatt cttctgtctt ttcttcttta ttagtcttgc 83460tagcggtcta tcaattttgt tgatcttttc aaaaaaccag ctcctggatt cattgatttt 83520ctgaagggtt ttttgtgtct ctatttcctt cagttctgct ctgatcttag ttatttcttg 83580ccttctgcta gcttttgaat gtgtttgctc ttgcttctct atttcttttc attgtgatgt 83640tagggtgtca attttagatc tttcctgctt tctcatgcgg gcatttagtg ctataaattt 83700ccttctacat attgctttga atatgtccca gagattctgg tatgttgtgt gtttgttctc 83760attggtttca aagaatatct ttgtttctgc cttcatttca ttatgtaccc agtagtcatt 83820caggagcagg ttgttcagtt tccatgtagt tgagtggttt tgagtgagtt tcttagtcct 83880gagttctagt ttgattgcac tgtggtctga gagatagttt gttacaattt ctgttctttt 83940acatttgctg aggagagctt tacttccaac tatgtggtca attttggaat aggtgtggtg 84000tggtgctaaa aaaaatgtat attctgttga tttggggtgg agagttctgt agatgtctat 84060taggtccgct tggtgcagag ctgagttcaa ttcctgggta tccttgttaa ctttctgtct 84120cgttgatctg tctaatgttg acagtggggt gttaaagtct cccattatta ttgtgtgggt 84180gtctaattct ctttgtagat ctctaaggac ttgctttatg aatctgggtg cccctgtact 84240gggtgcatgt atgtttagta tagttagctc ttcttgttga attgatccct ttaccattgt 84300gtaatggcct tctttgtctc ttttgatctt tgttggttta aagtctgttt tatcagagac 84360taggattgca acccctgcct ttgttttcca tttgcttggt agatcttcct ccatcccttt 84420attttgaacc tatgtgtgtc tctgcatgtg agatgggttt cctgaataca gcacactgat 84480gggtcttgac tctttatcca gttttccagt ctgtgtcttt taactggaac atttagccca 84540tttactttta aggttaatat tgttatgtgt gaatttgatc ctgtcattat gatgttagct 84600ggttattttg ctagttagtt gatgcagttt cttcctagca tcgatggtct ttacaatgtg 84660gcatgttttt gtggtggctg gtatgggttg cccctttcca tgtttagtgc ttccttcagg 84720agctctttta gggcaggcct ggtggtgaca aaatctctca gcatttgctt gtctgtaaag 84780gattttatgt ctccttcatt tatgaagctt agtttggttg gatatgaaat tctgggttga 84840aaattctttt ctttaagaat gttgaatatt ggccctcact ctcttctggc ttgtagagtt 84900tctgccaaga catgagctgt tagtctgatg ggcttccctt tgtgggtaac ctgacctttc 84960tctctggctg ctctcaacat tttttccttg atttcaactt tggtgaatct gacaattatg 85020tgtcttggag ttgctcctct cgaggagtat ctttgtggca ttctctgtag ttcctgaatt 85080tgaatgttgg gctgccttgc tagattgggg aagttctcct ggataatatc ctgcagagtg 85140ttttccaact tggttccatt ctccccgtct ctttcaggta caccaattag atgtagattt 85200ggtcttttca catagtccca tgtttcttgg aggctttgtt catttttttt ttcttttttc 85260tctaaacttc tcttcatgct tcatttcatt gatttgattt tccatcccca ataccctttc 85320ttccagttga ttgaatcagc tactgaggct tgtgcattca tcatgtagtt ctcatgccat 85380ggttttctgc tccatcaggt catttaagga cttctctgca ttggttattc tagttagcca 85440ttcatctaat tttttttcaa ggtttttaac tgctttgcca tgggttcgga cttcctcctt 85500tagctcagag tagtttgatc atctgaagcc ttcttctctc aactcgtcaa agtcattctc 85560catccaggtt tgttctgttg ctggtgagga gccgtgttcc tttggaggag gagaggcact 85620ctgattttta gagcttccag tttttctgct ctgtttgttc ctcatctttg tggttttatc 85680tacctttggt ctttgatgat ggtgacgtac agatggggtt ttggtgtgga tgtcctttct 85740gtttgttagt tttccttcta gaattcagga ccctcagctg caggtctgtt ggagtttact 85800ggaggtccac tccagaccct gtttgcctgg gtatcagcag cagaggctgc agaacagtgg 85860atattggtga acagcaaatg ttgctgcctg atcattcctc tggaagtttt gtctgagagg 85920agtacccagc cgtgtgaggt gtcagtctgc ccctactagg gggtgcctcc cagttaggct 85980attcgggggt cagggaccca cttgaggagg cagtctgccc attctcagat gtcaagctgt 86040gtgctgggag aaccacttct ttcttcaaag ctgtcagaca gggacattta agtctgcagt 86100ggttattgct gtcttttgtt tgtctgtgcc ctgcccctgg aggtggagtg tacagaggca 86160tgcaggcctc cttgagctgt ggtgggctcc agccagttca agcttcccgg ctgctttgtt 86220tacctactca agcctgggca atggcgggca cccctccccc agcctcgctg ccgccttaca 86280gtttggtctc agactgctgt gctagcaaag agtgaggctc tgtgggtgta gaaccctcca 86340agctatgtgt gggatataat ctcctggtgt gctgtttatt aagcccattg gaaaagcaca 86400atattagggc aggagtgacc caattttcca ggtgccatct gtgaatgctt tctttgacta 86460ggaaagggaa ttccctgacc tcttgccctt cccaggtgag gtgatccctc accctgcttt 86520gggtcacgca tggtgtgcta cacccactgt cctgcaacca ctgtctggca ctccccagtt 86580agatgaaccc ggtacctcag ttggaaatgt ggaaatcacc tgtcttttgc atcgctcatg 86640ctgggagctg tagactggaa ctgttcctat tcagccatct tggctccacg tatatccact 86700gccacacttc tttagtcata aagtgagtgc cttggtcaga ggcaatgctg tgtggaatac 86760catgatggtg gataaggcat tctgtgagtc catgaatggt agtcttggca gagcattgtg 86820ttcagtttag gcaaacccat atccagagta agtgtctgtt tcagtgagga caaacctctg 86880cccttttcag gatggaagag gtccagtata accaaccttc caccaggtaa cagactgatc 86940accccgagga atggtgccat attgagggct cagtgttagt ctctgctgct gtcaaattgg 87000gcactcagct gtagccatag ccaggccagt tttggtgagt ggaagtccat gttgctgagc 87060ccatgtataa cctccatccc tgccaccatg gccactttgt ttatgggccc attgggtgat 87120aacaggggtg gctggggaaa gaggctgagt gatgtccaca gaacaggtca tcttatccac 87180ttgattatta aaatcctcct ctgctgagat cacccattgg tgagcactca catgggatcc 87240aaatatcttc acaggttttg gccactcaga gaggtccatc cacatacctc ttccccaaat 87300ttctttgtca ccaattttcc aatcatgctt cttccaagtc cctgaccatc cagccaaacc 87360attggctaca gcccatgaat caatatataa tcgcacatct ggccatttat ccttccatga 87420aaagtgcaca accaggtgca ctgcttgaag ttctgcccac tgggaagatt tcccttcacc 87480actgcccttc agtgatgtcc tagaaagggg ctgtagtgct gcagctgtcc acttttgggt 87540ggtgcctgca tatcgtgcag aaccatctgt gaaccaggcc ctagtcttct cttcctctgt 87600caactgatca tagagaactc cccatgaggc catcggtgca ggctggggaa gagaaggtgg 87660ggtggcagtt gtggagacca tgggcatttg agctacttcc ttgtgtaact tacttgtgcc 87720tttaggacct gctcgagccc aatcacatat atatcacttc catttgatga tggaatgctg 87780ctgtgcatga cccattttat ggctacatgg gtcagaaagc acccagttca tgatagacag 87840ttcagttcac atggtgactt gatgacccat aatcaaatgt tcagtttcca ccaaagctca 87900gtaacaggcc aagagctgtc tttcaaaagc acagtagtta tatgcagaaa atggcagggc 87960cttgtttcaa aattttagag gcatctgctg tgattcacct gtgggggcct gccaaaggct 88020ccaaacagca tccctatctg ccactgatgc ctcaagcacc attggatctg ctgagtcata 88080tggcccaaac agcagagcag gttggacagc agcctggacc tgaggcagag ccttatcctg 88140ttctggaccc cactcaaaac tggcagcctt ttgagtcact cgataaatgg gcctgagtaa 88200cacacccaaa tgaggaaagt gttacctcca aaatccaagt aggcccacta catattgtgc 88260ctttttctta gttgtaggag gggccaaatg cagcaactta tccttcatct tacaaggagt 88320atcttgacag gccccacacc agtggacccc tagaaatgtt actgaggtag aaggtccctg 88380aattttagtc aaatttattt ctcatcctct ggcacacaaa catctcacaa ataagtccag 88440tgtgtttgct acttcttgct cactggatcc gatcagcata atgtcatcaa tgtaatggac 88500cagtgtgata tcttgcagaa gcaaaaagtg gtcaagatct ctccaaataa gattatgatg 88560caaagccaga gtttatatac tcttgaggta ggagagtaaa gatatattgc tagtcttgcc 88620agctgaaggc aaattgcttc tagtgggcct tatgaatagg aatggagaaa aaggcatttg 88680ccaagtcaat ggctgcatac caggtaccag gaaatgtgtt aatttgctca agcaatgaaa 88740ccgtatctgg tacagcagct gcaattggag tcaccactta gttaagctta tgataatcca 88800ctgtcattct ccaagatcca tctgtcttat gcacatgcca aatgggagag ttgaatgggg 88860gtgtgatgaa aatcaccacc cttgcgcctt tcaagtcctt gatcatggca ctaatctctg 88920caatccctcc agggatgtga tattgttttt gatttaccat ttttctaggt agaggcagct 88980ctaatgcctt ccatttggcc tttcccacca taataggcct caccctacca gtcagggagc 89040caatgtgggt gttcttccag ctgctaagta tgtctatgcc aattatgcat tctggcactg 89100gggaaatgaa cacaggatga gcctggggac ccattggacc cactgtaagt cagacctgag 89160ctaaaactcc attaattacc tcacctccat aagctcctgc tttaactgga ggaccacaat 89220gacgttttga gtcccttaga attaacgtca gctcagagct agtgtcctgt aatcccccaa 89280atgactgata atttcccttt ccccaatgca cagttacctt gataaaaggc tggaggtctc 89340cttgggaaaa aatgggagaa agattaacag cataaattgt tggtagtgta gtgggctcct 89400tccttaatgg gacccaactt cccctttatt caaggggttc tgtgtctgta aactggctca 89460agtcaaaaat tgattgaggg gccatgattc tctattttta taattcaaag tagtgttttg 89520tccatttgac ctggaagttt tctgcttata taaattaagt aggaaagaag taggcatcct 89580aacaatttca cttctgggaa cactgtgatt aattagcgaa tgccagaact ctacatgagt 89640cagactattc tgattgctgc tttgccacta ctgtctgtta tggtagttat gcccaccttg 89700cctttgatgg ttgagtgcca ccacttggcc tctgccacct tgggatccaa ttattcccat 89760tgtatttacc tttattagtt gagtgactgt ggttctcacc attggatctg atatgcagag 89820aattacgggg ctcttcaaag atgcaggtgc taccctcaca gatctatttt gcaaggtatt 89880ggtcaagggt atatcttctg ggcccttcca tctgggatga gtaggtctaa agtgactaat 89940ccactccacc atctcaatct ctctaagcct ttggatccct tcctctacat taaaccaaga 90000gtgatcagac atttccagct ccctcacagt ggccatcttt caatccatat ttcagctaac 90060taagcaaata aattattaga accttttttt aactccctga gctgcaatat taaatgcaga 90120gtctctactt agtgggccca aatcaacaaa ttcagcctga tccaactcta tgttccttcc 90180accattatcc cacaccctta atatccattc tcatgcctgt tctccagatt tctgtttata 90240taaattagaa aactcaagta gttcttttca agtatagtga acctcctcat gggtttcact 90300ctcaacctca cctcttgggg cccataagga ctttagtctg gttataggtc tagaagcaga 90360caggggtgtt gggggtggct cctgaggaga atcaacatta ttttgcctgg caactgctca 90420gggggggcca tcactgttgc cttaggcagc acagtgttta tctccccaga caaatatgga 90480aaggctgatg gcagcatggg tcagggagag gatgtcacca ctactgggga tggggaagct 90540gttttttttc tggcaaaaaa ggtttcctga gactttacaa actcagtgtc cccagcttca 90600tcagggtcct cccacatgtc ccctttccaa gttgcagggt cccattcttt tccaaacgat 90660gccctcactt taatagtaca tacctggtga ggctgtgcat gcacccttca ttggaagtca 90720gccacttgca caataagagc tcatatccat tttcccacaa tttcagctct ttctctgcag 90780gagataatac tttcactcag ggcaatctta gcagatttga ggctcagtat ctgcttctga 90840agccaggagt tagaatccct aagttcattt tctttcatca ctttgtccat tgaacttagg 90900agaaaccaac caacttcatt atgtttccac atatggtcaa aggtattatt tatagagtca 90960ctaaactcct tgcctctcat gagcgctgaa tcaggaatgt caaatgcatt tattttgctt 91020aactctctaa acagttcata gaaaggaaca ctgaatcagt gttctccata ctatcagaag 91080tagagtcctt agcatttttg agtctaatca tattaaacag ccactccaga aaccctaaaa 91140ccaatgaaag aactgcatcc ttaatgttct gttcctctag aaccactcct ggtacaaaaa 91200tctgtgttag ggttctctag aggggcagaa ctaataggat agagatatat aagaaggaaa 91260gtttattaag tattaactca catgatcaca aggtcccaca ataggatatc tgcaagctga 91320ggaacaagga gagccagtcc aagtcctaaa ctgaagaact tcgagtctga tgttcaaggg 91380cagggagcat ccagtaccag agaaagatgt aggctgggaa gctaggccag tttagtcttt 91440tcatgttttt ctgccaactt ttcatattct agctgcactg gcagctgatt agatggtgcc 91500cacccaggtt aagcgtgggt ctgcctttcc cagcccactg actcaaatgt taatctcctt 91560tggcaacacc ttcacagaca cacctaggat caatactttg catccttcaa tccaatcaag 91620ttgacactca gtattaacca tcacagaagc attatctact aataccacca tataaaagat 91680gcggatgtta gcatagttat cacactgttt cctcacacaa tccactaccc atggcttggt 91740ttctatggac atattattgg atctgagcca gcttattata atgttttaaa taatattcaa 91800gaattgttac aattgcattt tgaattgtta ttatttaaat ataggtcaat ttctaaatag 91860attcttgtcc atagtcagat tttacatcat gatttcctca ctcatgacat ttaaaagttt 91920atttgttaaa tttagccaat atatacaagt ctgcatttgg ggatgggtaa ttgaatgcca 91980tttttaattt ttgggtaatg gagacttttt ttatgttgcc tttatatttg atccataact 92040aggctatgta gtacatttct gggtcaaatc tctattgctt taaaactttg tggttttgct 92100tcattatctc ctagtatcta cagttactga agtgatacgt acaaccagct ttggacatga 92160tgaatgtttt aggctaaaga cttgggtgtt ctttggtctt tggaagtttt ctttaatact 92220ctctttaaat ttgtctgatt ccacttcttg gttccctttt caggaacacc aagtattcac 92280acagtggata gtcatctgtc ctctacttct aaaattttgt tatttaacaa aatgttataa 92340aagtagaatc acatttatgt aacctttttg gattgatgtt ttttgctcag cataatttcc 92400tggcaatcca tccaagttgt tgcatgcatc aatcatccat tcctttacat tgctaagtag 92460tattccatgc tatgtattta caacagtttg tttacccatt cacctgttga aaaacatctt 92520ggctgaatct agttcttagc tattaatatt ataaacaaag atgctataaa catttgtttg 92580acagtttttt gtgtgaagtt tttatttttc tgggataaat gcctgggagt gcaattgctg 92640ggtcatatga tagttgtgtg tttttctttg agaaactccc aaaatttccc agagtggatg 92700aagtatttta tgtttctatc agtaatgtat cagtgatccg gtttctacac atccttacca 92760gcatttggtg gtgtcatgat ttttaatttt agccattctg atagatgagt agtgatagct 92820tattgttgtt tcaatttgca tttccctaaa acctaataat tttgaacatc ttttcatgtg 92880attatttgct gtccatgaaa tatctgtgga aacagtgagt agaaggatgc tttccacagt 92940ctgagaagga cagctgggag aggtggatga agagaggttg gtatatgggt acaaaaatac 93000aattaagacc tctggtccat gctgggcaca gtggctcatg cctgcaatcc cagcactttg 93060ggaggccaaa gcgggtggat cacctgaggt caggagctta agaccagctt ggccaacatg 93120gtgaaaccct gtctctactg aacatacaaa aattagccgg gcatggtggt gcgtgcctgt 93180aatcccagct actcaggagg ctgaggtggg agaatcactt aaacccggga ggcgaagttt 93240gcagtaagcc gaggtcacac cactgcactc cagcctgggc aacagagcac tccagccggg 93300gcaacagagc aagactctgt ctcaaaaaaa taaaataaaa taaagtaaaa caaaataaag 93360agttccaatc cagcagagag tgtggtgaga tggtgcggga ccagctccct gggacttctg 93420agcctttgcc ctggccagtg ggggagctga gccaggatag gctgtagcct ctgccccgtg 93480acaccaggac ccttgtggtg gctgccacca ggacccttgg ggtgggtgcc tgggccttga 93540agatgcagca agcctccctt ccccctacac ctcagtgact gagggtggtg gtggcctttc 93600ccaggccgca ccctgccggg tatagacgaa gtccccactg agcctggatg cagtgccttt 93660gggtggtggt gggagtggca ggggcagctt ggtgactatc cgggtgtgag gattgggaag 93720gtgctcttgg gatgcaggac acgggctcag ccacctcttt aggccatggc gctgccagat 93780ccgggtcctg gtgaccactc tgtcccagct gggtgagact tacctagtcc tgaggacaac 93840agacaatggc cttaataggc ctggaaggtg agtggaggca cagaggagga cagtgtggaa 93900cagttagcag gcattgggct gttggtcttc cttctaccag acctggaagg acctcacatt 93960tggcctatgg gaaatgcccg ccacactttg ggaagattta ctatctgctt ctagaaggct 94020gtgtgtatat tatgaaaaag ctattctcaa ctcaccccca accttttaat agataacatc 94080tgtggggagg ctgacaagat ggctgaatag aaacagctca ggtgtgcagc tcacagtgag 94140atcaatgcag aaggtgggtg atttctgcat ttccaactga ggtacccggc tcatctcatt 94200tggactggtt agacagtggg tgcagcccat ggagggtgaa ctgaggcagg gtggggtgtt 94260gcctcacctg ggaagtgcaa ggggtcagaa aactcccctg ttagccaagg gaagctgtaa 94320ggagctgtgc catgaggagt ggtgcattcc agcccagata ctatgctttt cccatggtct 94380ttgcaaccca cagaccagga gattccctcc agtgcttatg ccaccagggt cttgggtttc 94440aagcacaaaa ctgggtggcc atttgggtag acaccaaggt agctgcagga gtcttttttc 94500ataccccagg gatgcctgga atgtcagcga gacagaacca ttcactcccc tggaaagggg 94560gccgaagcca gggaaccagg tggtctagct cagtggatcc cacccccatg gagcccagca 94620agctaagatc cactggcttg aaattcttgc tgccagcaca gtagtctgaa gttgacctgg 94680gatgctggag cttggtatgg ggaggggcgt ctgccattac tgaggcttga gtaggcagtg 94740ttcctctcac agtgtaaaca aaactgcaag gaagttccaa ctgggtggaa cacaccacag 94800cgctgccagg ctgctgtagc aagactgcct ctctagattc ctcctgtctg ggcagggcat 94860ctctgaaaaa aaggcagaag ccccagtcag ggacttatag ataaaactcc catctctctg 94920ggacagagca cctgggggaa ggggcagctg tgggtgcagc ttcagcagat tttaacatcc 94980ctgcctgcca gctctgaaga gagcagtgga tctcccagca cagtgtttga gcagactgcc 95040acctcaagtg ggtccctgac cccctggcct cctgaccggg agataccttc cagaagaagc 95100caacagacac ctcacacaag agagctccag ctggcatctg gcaggtgccc ctctgggaca 95160aagctttcag aggaaggaac aggcagcaat ctttgctgtt ctgcagcctc cactggtgat 95220acccaggcaa acaaggtctg aagtagatgt ccagcacact ccagcagacc tgcagcagag 95280aggcctgact gttagaagaa aaactaacaa acagaaagga atagcatcaa catcaacaaa 95340aaggacatcc actcagaaac cccatccaaa ggtcaccaac atcaaacaca aaaggtaggt 95400aaatccatga agatggggag aaaccagcac aaaaaggctg aaaattccaa aaaccagaat 95460gcctcttctt ctccaaaaaa tcacaacttc tcaccatcaa gggaacaaaa ctggatggag 95520aatgaatttg atgaattggc agaagtaggc ttcaaaggtg gataataaca aactcctctg 95580agctaaagga gaatgttcta acccaacgca aggaagcaaa gaaacttgaa aaaagattag 95640atgaattgct aactagaata acgagtttag agaagaacat aaatgacctg atggagctga 95700aaaacacagc acaagaactt agtgaagcat acacacatat caatagctga atcgatcaag 95760tggaagaaag gatatcagag attgaagatc aacttgcaga aaccctataa gccagaagag 95820actaggggcc aatattcaac attcttaaag aaaagaattt tcaatgctga atttcatatc 95880cagccaaact aagcttcata agtgaaggag aaataaaatt ctttacagac aagcaaatgt 95940gagacatttt gtcgtcacca cgcctgcatt acaagagctc ctgaagggag caataagcat 96000ggaaaggaaa aaatggtgcc agccactgca aaaacatgcc aaatggtaaa gaccatcaac 96060gctatgaaga aactgcatca agtaatgggc aaaataacca gctagcatca taatgatagg 96120atcaaattta cacataacat attaacctta aatgtaaatg ggctaaatgc cccaattaaa 96180agacacagac tggcaacatg gataaagagt caagacccat cagtgtgcag tattcaggag 96240acccatctca catgcaaaaa cacacatagg cccaaaataa agggatggag gaatatttac 96300caagcaaatg gaaagcaaaa aacaaacaaa caaacaaaca aacaaacaaa caggggttgc 96360aatcatagtc tctgataaaa cagactttaa accaacaaag atcaaaagag aaaaagaaag 96420ccattacata gtggctaaga agagctaact atcctaaata tacatgcacc caatacagga 96480gcacccagat tcataaggca agttcttaaa cacctacaag gagacttaga ctcccacaca 96540ataatagtgg gagattttaa taccccactg tcaatatcag acaggtcaat gagacagaaa 96600attaacaagg atatctagga attgaactca gctccagact aagcagacct aatagacatc 96660tatagaactc tccaccccaa atcaacagaa tatacattct tctcagcacc acatcgcact 96720tattctaaaa ttgaccacat aattggaagt aaaacactcc tcagcaaatg cagaagaatg 96780gaaatcataa caaacagtct ctcacaccac agcgcaatta aattagaatt cagaattaag 96840aaactcactc aaaacctcac acttacatgg aaactgaaca acctgctcct gaatgactgc 96900tggataaata atgaaatgaa gaaggataaa gatgttgttt gaaaacaata agaataatgt 96960accagaaact ctgggacata tttaaagcag tgtgtagagg aaaatttata gcagtaattg 97020cctacaaaag aacacaggaa agatctaaaa tcgacaccct aacatcacaa ttaaaagaac 97080tagagaagca agagcaaaca aattcaaaag ctagcagaaa acaagagata actaagatca 97140gggcagaact gaaagagata gagacatgaa aaacccttaa aaatcaatta atcgctctcc 97200ctctccctct ccctctccct ctccgtctcc ctctccgtct ccctctcccc acggtctccc 97260tctccctctc tttccacggt ctccctctga tgccgagccg aagctggact gtactgctgc 97320catctcggct cactgcaacc tccctgcctg attctcctgc ctcagcctgc cgagtgcctg 97380cgattgcagg tgcgcgccgc cacgcctgac tggttttctc tgcccggcca gccgccccgt 97440ccgggaggga ggtaggaggt cagccccccg cccggccagc cgccccatcc gggaggtgag 97500gggtgcctct gcccggccgc ccctactggg aagtgaggag cccctctgcc cagccaccac 97560cccgtctggg aggtgtaccc aacagctcat tgagaagggg ccatgatgac aatggcggtt 97620ttgtggaata gaaagggggg aaaggtgggg aaaagattga gaaatcggat ggttgccgtg 97680tctgtgtaga aagaagtaga catgggagac ttttcatttt gttctgtact aagaaaaatt 97740cttctgccgt gggatcctgt ggatctgtga ccttaccccc aaccctgtgc tctctgaaac 97800atgtgctgtg tccactcatg gttaaatgga ttaagggtgg tgcaagatgt gctttgttaa 97860acagatgctt gaaggcagca tgctccttaa gagtcatcac cactccctaa tctcaagtac 97920ccagggacac aaacactgcg gaaggcagca gggtcctctg cttgggaaaa ccagagacct 97980ttgttcactt gtttatctgc tgaccctccc tccattattg tcctatgacc ctgccaaatc 98040cccctctgcg agaaacaccc aagaatgatc aataaaaaaa taaataaata aaaataaaaa 98100taaaataaaa taaaataaaa aaagaaataa acacaaataa aatgttaaaa ttcccattct 98160acataaaatc atctatagat gtttttaaat atacaacatg attatcaagg acttaatctg 98220taccacattc ttgtagatgt aaagttaaaa ttcttgtaac catcactctc aaccctaccc 98280tatcttccag cataaatgag actttcacat gttttctctt gcccagatgg agacactaac 98340tgtaggcttt ggaaatgtct taaaaaaaaa aaaaaaaaaa agagagccag gcatcttcaa 98400acagagaaaa gctgtttgaa cacatttatc taagtctatc aaaagctatg attgttagca 98460agaggtaacc ttgtaatgcc aaggcagaaa actgccacca aaccacaaaa agcttgttca 98520actgctgaac ctaaaattaa acaatttatc tctcattaaa aaaaaaaaat caattaatcc 98580aggagctggt ttttttttaa agatcaacaa aatagataga ccactagcca gactaataaa 98640gaggaaaaga gagaagagtc aaatagacat aataaaaaat gataaaggag atatcaccac 98700tgatcccaca gaaatacaac ctaccatcag agaatattat aaacacctct atgcaaataa 98760accagaaaat ctagaagaaa tggataaatt cctggacaca tacaccctcc caagactaaa 98820ccaggaagaa gtcaaatccc tgaataagcc aataacaaac tctgaaattg aggtggtaat 98880taatagccta ccaaccaaaa aaagtccagg accagacgga ttcacagctg aattctacca 98940gaggtacaaa aggagctggt accattcctt ctgaaactat tccaaacaat agaaaaagag 99000agaatctccc taactcattt tacgaggcca gcatcatcct gataccaaag cctggcagag 99060acacaacaaa aaaagaaaat ttcaggccga aatccctaat gaatattgat gcaaaaatcc 99120tcagtaaaat tctggcaaac cgaatccagc agcacatcaa aaagcttatc caccatgatc 99180aagttggctt catccctggg atgcaaggct ggttcaacat atgcaaatta atgaacgtaa 99240tgcatcacat aaacagaacc aatgacaaaa accatgtgat tatctcaata gatgcagaaa 99300agaccttcga caaaattcaa cagcccttca cgctaaaaac tctcaataaa ctagatattc 99360atggaacatg tctcaaaaca atcagagcta tttatgagaa acccacagca gccaatatca 99420tactgaatgg gcaaaaactg gaagcattcc ctttgaaaac tggcataaga caaggatgcc 99480ctctctcacc actcctattc aacatagtat tgggagttct ggccagggca atcaggcaac 99540agaaagaaat gaaggatatt caattaggaa aagaggaagt caaattgtct ctgtttgcag 99600atgacatgac tgtatattta gaaaacccca tcatctcagc ccaaaatctc cttaagctga 99660taagcaactt cagcaaagtc ttgggataca aaatcaatgt gcaaaaatca caagcattcc 99720tatacaccaa taacagacaa accgagagcc aaatcacgag tgaactccca ttcacagttg 99780ctgcaaagag aataaaatac ttaggaatcc aacttacaag ggatgtgaag gacctcttca 99840aggagaacta caaatcactg ctcaatgaca tcagagagga cacaaacaaa tggaaaaaca 99900ttccatgctc atggatagga agaatcaata tcgtgaaaat ggccataata tccaaagtaa 99960tttatagatt gaatgctatc cccatcaagc taccactgac tttcttcaca gaattggaaa 100020aaactacttt aaatttcata tagaaccaaa aaagagcctg catagccaag ccaatcttaa 100080gcaaaaataa caaagctgga ggcatcacac tacctgactt taaactatac tacatggctg 100140cagtaaccaa aacagcatgg tactagtacc aaaacagata tatagaccaa tggaacagaa 100200cagaggcctc agaaataaca ccacacatct acaactatct gatctttgac aaacctgaca 100260aaaacaagca atggggaaag gattctctac ttaataaatg gcattgggaa aactggttag 100320ccatatgcag aaagctgaaa ctagatccct tccttccact gtatacaaaa attaactcaa 100380gatgggctaa agacttaaat gtaagaccta aaaccataaa aaccctaaaa gaaaacatag 100440gtaataccat tcaggacata ggcatgggca aagacttcat gactaaaaca caaaaagcaa 100500tggcaacaaa agccaaaatt gacaaatggg atctaattaa actaaggagc ttctgcacag 100560cgaaagtaac taccatcaga gtgaacaggc aaccaacaga atgggagaac atttttgcaa 100620tctatccatc taacaaaggg ctaatatcca gaatctacaa agaacttaaa caaatttaca 100680agaaaaaaca aacaattcca tcaaaaagtg ggtgaaggat aagaaaagac tcttctcaaa 100740agaagacatt tatgcagtca acaagcatag gaaaaaaagc tcatcatcac tggtcattag 100800agaagtgcaa atcaaaaaca caatgagata ccatttcatg ccagttagaa tggccatcat 100860taaaaagtca agaaacaaca gatactagag agcatgtgga gaaataggac cacttttaca 100920ctgttggtgg gagtggaaat tagttcaacc attgtggaag acagtgtggt attcctcatc 100980ttcaccacgg ggtggaggta cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 101040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 101100nnagaaacat agaccctgcc caacgcatat tcctgaaagg gatggcaagg tttttttaaa 101160ggttttatgg gtttaggtgt actctaaagg attttaaccc ttctaaaatt atttttgtat 101220aaaggtgtaa gaaagggatc caacttcact cttttacaaa tgggaagcca gtttttcccg 101280gccccttttt ttaaaaagga ttccctttcc cattggttgt ttttttcaag gttgtcaaag 101340atcagaaggt tgtagatgtg ggttggtaat ttttaggggt ttgttttggt cccattggtc 101400cataggtcgg ttgtggtacc agggccatgt tgttttggtt actgtaagcc tcgtagtatg 101460gtttgaagtc aggtagcatg atgcctccag ctctgttctt ttggcttagg attgtcttgg 101520caatgcgggc tcttttttgg gttccatatg aactttaaag tagtttttcc caattctgtg 101580cagaaagtca ttggtagttt gatggggatg gcattgatct ataaattacc ttgggagtat 101640ggccattttc atgatattga ttcttcctat ccatgagcat ggaatgttct tccatttgtt 101700tgtgtcctct tttattttgt tgagcagtgg tttgtagttc tccttgaaga ggtccttcac 101760atcccttgta agttggattc ctaggtattt tattctcttt gaagcaattg tgaatgggag 101820ttcactcatg atttggctgt ctgtctgtta ttggtatata agaatgcttg tgatttttgc 101880acattgattt tgtatcctga gactttgctg aagttcctta tcagcttaag gagattttgg 101940gctgagacga tggggttttc tagatataaa atcatgtcat ctacaaacag ggacaatttg 102000acttcctctt ttcctaattg aatacccttt atttccttct cctgcctgat tgccctggcc 102060agaacttcca acactatgtt gaataggagt ggtgagagag ggcatccctg tcttgtgcca 102120gttttcaaag ggaatgcttc cagtttttgc tccttcagta tgatattggc tgtgggtctg 102180tcataaatag ctcttattat tttgagatac atcccatcaa tacctaattt attgagagtt 102240ttcagcatga atggttgttg aattttgtca aaggcctttt ctgcatctat tgagataatc 102300atgtgttttt tgtcattggt tctgtttata tgctagatta catttattga tttgcgtatg 102360ttgaaccagc cttccatccc agggatgaag cccacttgat catggtggat aagctttctg 102420atgtactgct ggattcggtt tgccagtatt ttattgagga ttttcgcatt gatgttcatc 102480agggatattg gtctaaaatt ctctttgttt gttgtgtgtc tgccaggctt tggtatcggg 102540atgatgctgg cctcatataa tgagttaggg aggattccct ctttttctat tgattggaat 102600agtttcgcca aactgacatg atttttaaaa ttccctatag ggtcaatgtg cagatttaaa 102660gatccctaca ctcagtagtc ctgggatagg gtcgggggat ccatatgttt aacaagcagc 102720ctagagtctc ccgatagcct gggcaagctt taaaaagccc tgcggacatt cggatgaaac 102780aaaggtgcac agaacctata tctctgccat ttattagctg ggggccttag gcaaggaagg 102840tagtcaacct ccgtattcct cagttcctca actgtaaaat ggggatgatg actgtggctc 102900cctcgtgggg ttgtgacgga ggaacagggg acatatgtcc tgggatcagc actggcaatt 102960tggccattct taccatagca cctgccatct tggtgacagc ctggacacaa agccttcaca 103020cctttgaagt ggccatagag agcatgagat atggatgggg aggaggcatt ccaactaaag 103080acccctgctt tgggccaaaa acacatgtat gtctggtgtc tggagtcctc tggttatgcc 103140ttttttaaga taccggttgt cctgcaattg tgcgttgaga atccactcta tggccaacat 103200atcatcaaag cgtttggctc agctaggctg ttcccccaac ccaaggaccc catgaggaaa 103260ggagtgaggg ttccaccccc agcaccagcc actggagggg ctgcccagag tccctcagtt 103320ctcgtaaact tacgctttgg agtaattcag aacaaagtcc actccttttt cactatcaaa 103380ctggatatca cggggtaaat ccttgtggca tttggcatcg atgctcaagg ggaagccagg 103440gttccactcc atccatctga gcatgagaaa ggagaagaaa ttgagttaaa tgccagcctt 103500tggtctgccc cctcagctga gggccgccca cactgtcaga cgatgttaca cagggagatt 103560gatgtactca aaataaagtc aacacatcat aggctgaccc tgtgtccagt aaggttctgg 103620gacctcttcc aaagtccagt cgcaccaact ttgctgtgcc ctgagcatca gagtgggaac 103680cctgagactg ggcttatagt ggtacaggtg tgcaccactg cataaaaagt tctgtaggta 103740gtctagggag acctcactta ctagaggtga cattgaacag tagaggcctg ccttgccagt 103800cactggaggt agaaggtatt aaggctgccc agccacgccc aacctggaca tccaccattc 103860tgccaacttg atcagtgtgt gccctggact gcgtgcatgt cctgcatgtg gcatttgggg 103920aaatgggaca gttcttctgc cttcacaata ggacgacacg gcagcaggtg gcctgggaga 103980gggacccagg cacagatggc taaaaggctt ccctctctca cctgctggcc aatagcagct 104040ttagagatta ttacaatcaa aacagcttcc ccaaagacaa gccacggact gcatccagta 104100aacatgctca gatcctgaca tccttgaaac gtatcccgtg gcattgggca tttgtgtttt 104160tgaaaaatgt ttattttgct tttttgttta cttattgagt aaacttaaat tgtgaaaatg 104220tcagaggtgt ttgaaccaca gcaactccat cttgaatagg ggctgggtaa aataaggctg 104280agacctactg ggcagcattc ccaggaggtt aggcattcta agtcacagga tgagacagga 104340cattgatgca agatacaggg cagaaagacc ttgctgataa aatgggttgt ggtaaagaag 104400ccggccaaaa cccaacaaaa ctaagatggt gatgaaagtg acctctgttc atcctcacca 104460ctcattatat gctaattata atgcattagc atgctaaaag aaactcccac cagtgccatg 104520acagtttaca aatgccatag taatgtcagg aagttaccct atatggtcta aaaaagggag 104580gaaccttcag ttcctggaat tgcccacccc tctcctggaa aactcatgaa taatccactc 104640attgtttagc acatgatcac gaagtgactg taagtatgct cagtcgagta gcccatgcta 104700ctgccttgcc tatggagtag cctttcttta ttgctttact ttcctaataa acttgctttc 104760actttatgga ttcaccctga attctttcct gtggaggtcc aagaactctc tcttggggtc 104820tggatcagga cccctttctg gtaacaaaaa caaaatgaaa tcctgttagt ttctttgggg 104880agggagaatg attttgtgtt tgtttaaaaa ccagatttta cattttaggg ttttagcttt 104940ttcatttata ttcctaattc tccttccttt taaaattttc ttttgagagg aaagtaaaat 105000atcgagattt tgggtcttaa aaaagtcctg agttatttta atatgatctt gtgctttgac 105060tttccccaaa tcccaactgt cacagtgtcc tcttggccca gtagcctctc cttaggatca 105120caagaagtga acagggaccg ggtatgatcc ttctgacagc aaaggcattt tgtcaagtgt 105180tttctgttat gtggcacctt acatttctca ctgttggttt atttagaata ttctacgtcc 105240tcagatttta agaaaacctt ccatttttgc tgtttgtatt aatgccaatg atagtatatt 105300aacaaggata aaaatgtgga caagtctaca cattttttgt ttgtttgttt tggaaaatga 105360ctcaaggcat ctccaatata tatctaaaat tgagccattc agtagcctca ctggtagtaa 105420tgatcaatta attagctatc attagtcata tcaatttaaa tattactgat gattttctct 105480ttgcctggac agttcaaggg ctctagttaa ttttaaaagc tcagtggagc tagatgtttt 105540tcagtatgta aacaatgagc catgcaaggg catctgtgcc agcaaatggc attaatgttt 105600tcttaagtga atagtccact tggacggatg tggaatactt cttcagcacc aaaaagacct 105660tcttgcccca tgccctgccc tggtctatgg agggtggcag caccaatggg cagaaatgcc 105720ccacttgtgt ggttagaaga tgatggcctc acagcacatg gagacaggac agcttgcttg 105780ctagttcact actgcccccc tgaaaccaaa tgtttatgtc ccttccactg caggtcactg 105840ctgcttaaga gaccctgaca ggctgacctg cacaggagcc gaaggcctgc atggccacag 105900acattaactg gtggagatgg aatcccactc cattgaggaa gaccaagtgg aacacccagg 105960cagaaggaac aaagaagtag aaaggcattt aatttattgg aaaaaaaatg agagagagag 106020agaaagagag agagagaaag caagagagac agagagacag agagagagag agagagaaag 106080agagagagag aaagcaagag agacagagag acagagagag agagagagag agagagacag 106140acagacagag agatcctaag gggatgcagt tagtataatt ggagtgtaaa aagactttct 106200gtaaccacaa acatgatttc tgaatgaaga cttcaataga gaaacagaca aagagaataa 106260tcactgtgag atgtttggtc tcttgtatta aaagaaaagc tttagacaaa ttaaatttag 106320cagtttaatt gagcaaagaa caattcaaga atagggcagc ccctaaacca gagcaggctc 106380cgagtgactt tgatgctgtc tcatagttgg ataggattta tgggcagaaa aaggaacgtg 106440atgtacagaa aatggaagtg aggtacagaa acagccagat tggtgatggc tcagcatttg 106500ccttacttga gcccagtttg aacagctggc cactgtgagt ggctgaagtc tggccgctgt 106560gattggctga gacttggtta cttgctatga gagtaggtga cagtctgttt acacatccag 106620ttaggttaca gttcactatg tacagagaaa cttttaggct gaatttaaaa tatgtaagga 106680gaaaagttta agctaaactc attaacacct agaagagaga aggtgttcat tcgacaacta 106740attatggagc acctactatg tgccaggaag tgctttgtgc aaaatagaag aacatgtaac 106800ccaaatacag ggtgaaacta caaagaaatg gaagccataa atgaaactat gagagacata 106860aaccaaaaat aaaattctaa gcccctgaac tgactgatgg accctcccct tggccaaggg 106920cattccaaag ttaacctgaa aaattagttc aggccatgat aggaatgggt ggctaaacgt 106980gcctcattgt actctcctcc tcttggaatt caggcacaac tgatcagcat tcacatcaac 107040acagagatgt taagcccatt agaacagact ctttaagatt gataagaaac atttacaatt 107100gattctacct gaagcctgct acctggaggc ttcatctgca tgataaaaaa ctttgtttcc 107160acaacccctt atcttaacct agacattcca agtttttaga taataattct tttaagcagt 107220tgccaatcag aaactctttg aatctgccta tgacctggaa gctcccctcc cccaagttgt 107280cccacctttc caaaaccaaa ccactgtcca tcttacatgt attgactgat gtcttatgtc 107340tccctaaaat gcacaaaagc aagctgtacc ccaaccacct tgggtgcatg ttctcaggat 107400ctcctggggc tgcatcatgg gccactggtc actcatattt ggctcagaat aagtcttttc 107460aaatatttca cagagtgtga ctcttttcat taacagacat gaaagccaga tacagatgta 107520atatgcaagg agtaaaagct ccaaaaggag agagagtaat atatggagaa gtgaaaaaaa 107580tcaaagaaat aaaagaagaa ctcctttaag ctgaaaaata cttcaatatc ttaagtctgt 107640gaaaggaaat taaattttag gaccccaaac tcatttagcc aaagggaaat gtcaagctgg 107700gaactgtgtc atgcaaacct gcctccccct tttggttcct aaataagatg gctatgatga 107760aaagctacat gtctccacca tattttgctc acaaggaaat tcctagtgaa ccgttaaaat 107820ttcaccatgg caatgcaaat tgatagctta tctttacaag tgcagtcacc caccagatac 107880aaatgcatat ctgattgttc ccctgcctta ctttatctat gttctcttat gtaaaatgca 107940gactccctgc atttttcctc tgcctcattt gtttatgtca tcttatgtta aaaaaaatgc 108000agattcactg agccagagaa aggcatgaat aactattttt ccctgccctg ctcttacatg 108060aaaattgtgt acttctcaat atcccgccct tttcccttta aatttggagc cctcaaaatc 108120accttcagag aaaggcatag tgctgtctcc cgggcatgcg tccttaactt tcgcaaataa 108180atttcctaaa atgattgaga cttgtctcgt catttttctc aatgacaagt cctaggtggg 108240ggggaaaaaa aaaaaggaaa aagatacacc taggtatata ctgaatacat tttaaaatgt 108300ccaagagaca ggagaaacag agacagagag aaggaaagag atgcagagag tgaacgagag 108360aggaagaaag tgggggaggg agggagatag aaactctagg aaaagaaaca aactatcatt 108420agatttctcc tctgtcattc tgaaaatggg aagactgaag tgtatacaca ctttttttag 108480atcgtcatct aagcctctca tattgtcatc aatgagggca ggggaatttg tttgagggga 108540tgggtagggg gacaggacga caagcacaga ctcagatctt acactacaca tggaccttct 108600gaggaaatta attgaacaag ttcagctgaa caaaaattaa attagaatga atcaataaac 108660tcctaaatac agcaaagaat atgcattata aaactacagc aaatataata gtaaacaaag 108720aaacaaaaat atgagaggga tgttcatcac tactaccact acttgttttg ggaattccag 108780acatggaata agaaaagaat atcagtggtg tagataatgg aaagtaagag acaaaattat 108840taacatttag ataatatgag cacattaact aaccagactc actaacaaca aacaaacaaa 108900aacaagtgat tagaaccaat gaaagaattc agtaagggac caacacacag taaatgtaag 108960aaacaagtaa tttacattta tcccagcaat atttttgcaa acagaaaaat atcctactca 109020gaacagcaaa tgctaaatgt agagtagcta gaaaggtgaa ggacctgcat gaagaaaact 109080agaaattcta tggaagtacg ttgacacagg atctagagag gtggaaagac acgctgtgtt 109140cctggatgga aagactgaat ctcagtaaaa tgccaacaca taacacagtt atgtgaagtt 109200caaagtaatt ctcattatga aaaacttaag aaagaacagt gagatatgta acagacatta 109260acatttatta gaaagttatg ataaccaaaa gctggtgctg gggtctcgag tagaaaaata 109320ggctaacaca taaagtgcgg aaacagaccc agagactatg tatacataat acaaaactcg 109380ccaacactta tccagtgctt tctatgtgcc tactactatt ctctgtgttt tcatggttta 109440acctattatc tcaaacccct aaaaggaagg cactgttatt tccctcattt tacagatgag 109500ggaagtgagg cacagacaga ttaaacaact ttcacaggac tggagaaagt gccagtaagt 109560gtgagttgag cccagacact cgaatccaga gctcatgact gccatcctat gccagcttgt 109620accgtgggta tcatcatccc tgtcatccaa gtcaggagga aagacatcat ccctgtcatc 109680caagtcagga acgaatgcta ccgggagagt tccacccagg tatgtatgaa aacacgtggc 109740aggagtgggg catggtggcg cacgcctgta gccccagcta cttgggaggc tgacgtggga 109800agatggattg agcctgggtt agtttgaggc agcagtgaac catgatcatg ccactgcact 109860ccagcctagg aaacagagca agactctgtc tcaaaaaaaa aaaaaaaggt agatgaatga 109920aagctctaaa ctcaaagggg aaattataaa agtattagac aaaattacgg gggaagtatt 109980tgtgtaacac tgggatggaa agggttttct taagcaaagg aggaaaccca aaagttatag 110040atgtaaacaa tacatagtta ataaaataaa gaactaaaat ctcctgtggc aaaagcaaca 110100gaagacaaat aataaatgct atacatatgt aacaaacgga tactatcctg atatagaaag 110160agctccttta gattaataat tgaaagaaca atagggaaaa aaagcttata aataggcaat 110220gcagtgatgc ttattagtgg tcaggggagt gcagactaaa acaaccacaa ggtcttaggg 110280atttttagac tgtcaaacaa ggtcatcggg tactgacaaa tgtatggatg ggcaaaagta 110340tcttttcata cacagctggt aggaccgcaa attacgatac aattttgtga aaggattttt 110400gtcggtattt cttaagatga aaaaatgctg atttgttcca gcaatctgag ttctggaaat 110460ggatctgcca ttaattaaag caccagtaca tcaagatgtt tgtacaaggt tggttctggc 110520agcattgttt gtactgcaat acccaaaaca aacaaacaca tctacaaatg ggaaatgaac 110580tgaacgtcag tgaatagggg aatggctaag taaattatgg tgcattccca gaaggcaaga 110640aatagtagca gtttaagaag gggaaaatat atgagatact atttcacacg caagaatgaa 110700aacatctgaa gatgcgaagt gttgatgatg cagggaaaca gtgaatctca gaaacagtta 110760ctggggtgtc tactggcaga atgcctttgg aaaacaatca gcaatatcta gaaagcttaa 110820aaacacgtgg tctgcaaccc tgcaactcta cttcttggaa tattccctac agtaactctt 110880ccatgagagc acagagagat gtgtgagaaa gctcactgtc gcttggtttg caacagctag 110940aaattaaaga cagcccgcct gcccatcagc aggagagcgg acgagcgcat gggttattca 111000gatactgata tgccatgaag aagttaactg catgaaccac atctatctgt gtcaaccaga 111060tcctcttcaa tacttagtga tgagtgaaaa gtgcaggtca cataatgatt agattaaatt 111120atatcgatta acaaacacct acactttgcc atttactgct gtgtgtgagt gtgcatgtaa 111180cacagacaga tgtggtcaca gtgggagagc acaagctgtg ggctatgacc tgggtctgct 111240tctccttctc cctgcgtggc tgagtgagta aggagggcct gcaagaagcg ccagctttct 111300gacatagtgt ggactattct tagtcattgc tttacttcca aactctgatc tggaagcctg 111360agaaatcttc tgagaaagag gaactccaag attgtgcaga cagtgcaaag ttcagtgttt 111420cctgaacgga agcaaaggag ggtttctgaa aaggacagat gagtggaagg aggaagaaaa 111480aaatgattaa atctaagaaa gggggaaacg atctcaccag aagccccctg ctgcaagatt 111540atgataatgt taagtagctc ccagataatt gggctagttt gtgttatctt agatgagtag 111600atagcaaagt ctaattagag ccccactaat tcatactaaa gtgttagtgg cttataacaa 111660attaaatgaa ttttatgggc attttatctt aacccagatt cacaggccag aagtgctctg 111720agaactggct gcaggggctt tgccataggt gtctgaaaca gggagggaag ccaaggaaga 111780agcatagaaa caacagccct ttgggatggg aggcagagct caagcacaac gtgtgcactg 111840caccaggtat ggaagtctgg aaacacatca tatggtgcct gagctgagag gcttgtgcac 111900aaatactcta aaacaaatca cacatttata aacccttaac atgacccaca gcacagcagg 111960ttactctccc acacttgatg ctaatggcat gtaagtttta tttttgattt ttcaattggg 112020aaatagaata tataaaatat gtatggatat gtatatatgc acacacacac tcacacatgc 112080agaaaaatgt acaaaacacc tgtacgaaca ccatccaggt aaaaaatcag aacattgtcc 112140gtaccctcac ccccaccttt cccaatcaac cctccttcca tccccggtaa tgaccatcct 112200cggctttatg ataatcattt tcttgttatt gtttaaggtt ccgctatgac acacctccct 112260aaacaatttc attccaaagt tatgaattgg ccagacacgc tcctggcttc tttccttcaa 112320cactgcgtca gtgaggtttg tccatgttag cagcagtttg ttgattttcc ttgcagtgta 112380gaattttcct tgacaatttg tctgttctac tgtttgttgg acattgagtt gttgccattt 112440tttggccatc atgagtaatg ctgttgtgag tatccaggcg cacatgcaca caaatttgga 112500tcccactata ggatttctgg gtcatagatt ataatgtagc ttcagtttta tgacatattg 112560ctaaattgtt ttccacggag gtcttaccaa cttgtcctcc tgccggtggt atatgagtgt 112620tcccagccct ctggatcttc tttaatactc aggtgttgtc agttgttgaa gtgttgccca 112680tctgggggca tatgtgtggt agtagcagca gttcactgtg atcttctgat ttccccgagt 112740cctaatgaag ttgaacacct tttgtatact tgctgcttat attttgtaca gtaagtactc 112800aaacttcttg cccattttct agtgaatgac actccccttc ttaaagattt gtaggtcctt 112860cacatactag gaataattct cgtcaatcac tccatgactt gccttcactc tctcctggtg 112920tctttcaatg aacagagtgt ccttatcttg agggaggcaa acttaccaat cttttccttt 112980gtgtttaata tgttctgagt cttgtttgaa aaatcctttt agcttgagac ctggaagaat 113040aatcacctgt agtctaaatg tcttacagtt ttgcctttaa cttttaagtc taatcaaccc 113100caagctgagt ctcctgaatg gcaagaggtg gagtctgatt tcttctcctt ctccttcagc 113160cctccgtgct ggctggcccc tgtcctcgag atgcatgtcc agtggcccag tgccacccat 113220cagccgagcc ctccgtagca gacccagtac ccacatacgt gtggctgttt gttgtttctt 113280tattctgttt caaggaatgt ttttgatgaa aagggggatg aagctaagac agttttgagg 113340tggggcaatt gtgttcttgg tgtgggattt ctcacccctt gacagcagtg ggtggctggg 113400cataggggaa gaaggagcta cagggttgag ggatgctgtt gcaacttaga aaaattaaga 113460aaaatgaaga tattgctcag ttatggcaag tgtttgatga aacagggacc catacatggc 113520tcgtgaaaac tggttaaaga tttttttttt tttttttttt gagacggagt ctcgctctgt 113580cgcccaggct ggagtgcagt ggtgcaatct cggctcactg caagctccgc ctcccaggtt 113640cacgccattc tcttgcctca gcctcccgag cagctgggac tacaggcgcc cgccaccacg 113700cccggctaat tttttgtatt ttttagtaga gacggggttt caccgtgtta gccaggatgg 113760tctcgatctc ctgacctggt gatccaccca cctcagcctc ccaaatggtt aaagattttt 113820gaggagaaat ttgtcaatgt atactaataa cttttactat ttggtaattt catatctggg 113880catctgtcct aggaaaatac ttaataatgt atacaaattt cacatacaag ggttttcact 113940ttgcttataa gaatacaaaa attagaaacc tcctaaatgt ccagggaagc tgggtgttta 114000agtgaatgat gaggcatcca tccaatggaa tagtacatag aaactagaat aatgtttatg 114060atgagatttt gaaaatatgg gaaaatgatt tttgctacac tgctaaaggg cagcaaggag 114120gatgcaaagt ggtatatata gcacaatccc aaattttaaa aagtgaataa gacaaagtga 114180aaacaattaa acatgaattt ttatgaaaaa gttcataaaa ttcataattc atacagaaaa 114240taggtatttt tctggctcta tattattttc cttcttttac ttttttgagt taaaaaaaca 114300acccagatat actacatatt ccttgtaaga agttcaacta ttgagggata tgtagaaaag 114360tatcacaata ttggctccca aaggcccctc cacagcccag gtaacttcac tgtccatgta 114420gaatgattcc ttccactttc ttcccttgga aggaggcaca gactcaaatg tttccagggg 114480ccaaagcagg aaatgaaatg aaagaaaacc ttgatataga caacagacaa gtgtggactg 114540gggaagcctg aagaatatct ctgtttactc ataagtcaat aatgatccat caaacacttg 114600tagaggctgg atacagccca tcagccaccc atttccagag gagcactctg taatgtgtta 114660ttatcttcca aaaagcttta ctgagataca tttacatacc attaaattca ccccttaaaa 114720atatgctatc aaaagatttt aatatatttg cagagttgta tgtgtaacca tcattataac 114780ccaattctga aataatctca tccccagagg acacctcgaa cccattaaca ttcactcctt 114840gtttcccctc cccaactcct agtgctaggc aactactcac ctactaacct gactctgtgg 114900gtttgcctat tctggacatt tcatacagat agaatcacac aatatggggt cttccgtaag 114960tgagttttcc atttagcata tttttgaagt tcattcagtg tttggcatct atctgtagtt 115020catttctttt cttttctttt ctttttttga aatggagtct tgctctgtca cccaggctgg 115080agtgcagtgg cgggatctag gctcactgca acctccgcct cccgggttca agtgattctc 115140ctgccttagc ctcccaagca gctgggatta caggcgtgca ccaacatgcc cggctaattt 115200ttgtattttt gtagacatgg ggtttcacca tgttggccag gttggtctca aactcctgac 115260ctcaagtgat ctgcccgtct caacctccca aagtgctagg attacaggtg tgagcctccg 115320cgcttggcat tcattccttt ttgatgccaa aaaatatccc attgtatgga taaactatat 115380tttgtttatt cattcctcag ttaatggaat ttaggttgtt tctacttttt gactattgtg 115440aacaatggtg ctataaatat gtgtgtacaa gttttgtata gacatgtggt ttcaatattc 115500ttgggtagag gcctaggagt agaattgctg ggtcatattg tagctgggtt tcagattttg 115560aggaaccact aaactgtttt ctgaagtggc tgcatcatct tacattccgg tagcagcatc 115620agggttccag tttctccaca tccttggcag cacttgttac tatctgtctt ttttattcta 115680accattttag tggttagaat tgtatcttgt ggttttgatt tgaaatttcc tgatgattaa 115740taatgttgag catattttca cgtgcttatt ggagattcac aaattttctc tggagaacta 115800tttaattaaa tcctttgcta atttttaaac ggggtttttt gtacagaata ttttaaaatt 115860gagttgtcag agttctttat atattctgga tacaagtccc ttatcagaca tatgatttag 115920atattttctc cattctgtgg attgtctttt cactttattg atgatgtcct ttgaagcata 115980gatcttttac attttaatga catctaactt gtctactttc ttttgctgcc tgtgctcttg 116040gtgccatatc taagagactt tgccgaaccc aagattatga agacttactt gtatgttttc 116100ttccaagagt gttatgttgt tagcccttac ttttagatct atcatccatt ttgaattaat 116160ttttaaattt atctagcagc gttattgaaa tataatgcac atactgtaaa atttatccat 116220ttaaagtgtt caattcaagg atgtttagta ttaattttta cacaatggac aaccatcagt 116280tttagaacat tttcatcccc ggcaaaagga acactgtatc ctttagctgt cactcccatg 116340accacttcct cattccctgc ccctaggcaa ccagtaatct actttatatc tgtacagatt 116400tttcttttcc agaaatttca tataaaagga atcatacaat atatgatctt ttgtgactgg 116460tttctttcac ttagcataat gtttttgaga ttcatccatg ttgtaacaag tgtcagtatt 116520tcattcttct ttcatggtca aattatatag atatactata ttttatccat tcatccatta 116580atggacgttt gggttgtgta cactttttga tagttacagg tcatgctgct ataatcatgc 116640atgtacaagt ttttgtgtgg gcatatgttt ccatttctgt tgggaatatg cttaagagtg 116700gaaatgctgt catattgtaa ctctctgttg aacagtcagg agctagcaga ctgtttccca 116760agcagctgtg ccattttaca ttcccaccag cagtgtatga gggttctgat ttctccacat 116820ccttgtcaac acttgttctt gtcggatttt tttattctag ccatcctagt atcaagtggt 116880atctcattgt ggtgttgatt tgcatttcct agatgactaa tgggtccttg gagaaatatc 116940tattcagatc ctcactcatt tttttcattt tttaattgga ctatttgtct ttattgagtt 117000gtaagagttc tttataaatt atggatggaa ggcccttatc agatatacaa tttgtaaata 117060tttcttccat tctgtggttc atcttttcac tttcttgatg gtgtcctttg aaacataaag 117120gtttgtaaat ttgatgacat cactttatca ctttatctat ttttcttttg ttgctgatgc 117180ttttggtgtc atatcaagag tcctttgtca aatccaaagt catgaaggtt tactcctgtg 117240ttttcttaaa agagttttat agttttagct cttacattta ggtaggtatt tgactcattt 117300tgtgttaatt tatgtatatg gtgtgagcta aggatccaac ttcattcttt tgcatgtggc 117360tatccagttt tgctaggacc atttgttgaa aagactattc tttctctgtt aaactgtctt 117420gctgcttctg tcaaaaatta attggccata agtgtaaggg cttatttctg gactctcaag 117480tctatttcat tgatctatat atctatcctt atgccgatat cacattgtct tgaatactat 117540agctgtgtag taattttgat attgagaagt gcaagtcctc catctttgtt ttctttttat 117600caagattgtt ttggctattc tgggtccctt ccatttccat atgaatttgc agaattgaca 117660gcttgtcaat ttctgcaaag aaatcagcta gaattttgat aagggttgtg ttgaacgtgt 117720acattaattt gtggggtatt gccattttaa caaaattaag tcctctgatt catgaacatg 117780aaatgtcttt caacctattt agatcttctt taataatgtt aaacaatgtt ttgtagtttc 117840attgtacaag tctcagacta ctttgttaaa tttattcata aatgtttact gtttttgatg 117900ctattgtaaa ttgaactttt tcttactttt atttcacata gttggttgtt aatgtataga 117960aaaacaattg attatcttgt atcttgcaat cttggtggtt tattagttct aataatttct 118020tttggtgaat gccttcagat tttctatata taagatcatg ttatttgtaa attgtggtag 118080ttttacttac tgtactccat tgtgggtgcc tttacttatc tttttattcc ctaactgagc 118140tggctagacc ctccagtaca atgttgaata gaagtagcaa aagtgaacat ctttatcctg 118200tttctggatt cagcctttca tcattaagta taatgttaac tgtgaatttt ctttttttgt 118260gtatgtgggg ggacggagtc ttgcttgtca ccatgctgga gtgcagtggc atgatctcag 118320ctcactgcaa cctccgcctt ctgggttcaa gtgattctcc tgcctcagcc tcctgagtag 118380ctgggactac aggcgcatgc caccacacac agctactttt tgtattttag tatagacggg 118440gtttcaccat gttggccagg atggtctcaa tctcttgaca tcgtgatcca cccgccttgg 118500cctcccaaag tgctgggatt acaggtgtga accaccacgc ccagcctaat tgtgaatttt 118560caaaccttta tcaggttgag gacattttct tcaattccta ttttttgagt gttttatcat 118620gaatgggtgt tggattttgt caaatgcctt ttttgtatgt ctattgaaat gattatgtac 118680tttgttttgt tttgttattt actctattga tatggtgaat tcaattaatt gatttttgga 118740tgttaaacca actttgcatt cctgggctaa atcccacatg ttcatattgt ttaatgcctt 118800ttatatactt ctgggttagg tttgctagta tttcgttgaa aatgtttata tctatattgc 118860taagggatat tggtttatag ttttcttgtg atgcctttag ttttggtata atatgggtat 118920gagacaatga attgggaagt gttccttcta tttcttaaaa gagtttatgc aagatcagta 118980ttaattcttt aaatgtttga tagaattagc cagtgaagct atctgggtct gggcttctat 119040ttgtgggaaa ttttagaatt agtaattcaa tctctttggt tgtgattgtt ttattcagat 119100taactatttc ttcatgagtc agttttggca gtttgcgtct ttctaggaat ttgcgcattt 119160catttaacta gtttgttggc atgcaattgt tcatagtatt cttttttatt aattttaatt 119220tctgtattga ttttccctca ttttatgagt ctcatttttc cttttttatt cattttaatt 119280tctgtattga ttttctctta ttgctcattt taatggtttg aatcctctgg tcagttggct 119340gaagagttgt caattttatt gctgatcatg tatcactttt ataattgaat tgttataaga 119400agacataaga agtaagaggc aacccctaca atggagaaca ttaaggtaca ctcaagcttt 119460tctgcccatt cttcctacat gccaatatgg ttgatccaaa gttattttca ttactctcct 119520ctccatctct tgtctggagt tgtaggtaag gtgaagtttg gggattgtgc acccacactg 119580ggagctggtg ccttggtgac tttccagcag tgacaggcca tacactgtgg aaagggttta 119640tttcatacca tgctcttgag atagaagaaa ccatggcccc gtggccactc gatctgatgt 119700cataactcac cgatattgtt tttgccgtgt ttccagttct ttacgtcggt gttgcttgag 119760aatgtgaatt tggtcatctc gggccaactt tgctgtaaag aaagaacaac atgagaggag 119820cctgaggaag aacaataaca atgcccaatg ccaagtgttc aggcttccct cagatgccct 119880gcccatgccg agtgctttat gtgcatgacc tcatttgagc ctcagagcac ttccccaatt 119940aatgatgaag aaactgaggc ttagggaagt tgtgtagctt tgctcttgaa ctttacccaa 120000ggtcacacac ctaattagtg aattttcttt tttttttttt aattctactt taagttctag 120060gatacatgtg cacaacgtgc aggtttgtta catatgtata catgtgtcat gttggtgtgc 120120tgcacccatt aactcgtcat ttacattagt tatatctcct aatgctatcc ctccccctta 120180cccccacccc atgacagacc ttggtgtgtg atgttcccct tcctgtgtcc aagtgttctc 120240attgttcaat tcccacctat gagtgagaac atgtagtgtt tgtttttttg tccttgcgat 120300agtttgctga gaagggtgat ttccagcttc atccatgtcc ctacaaagga catgaattca 120360tcctttttta tggctgcata gtattccatg gtgtatatgt gccacatttt cttaattcag 120420tctatcattg atggacattt gggttggttc caagtctttg ctattgtgaa tagtgctgca 120480ataaacatac gtgtgcatgt gtctttatag cagcatgatt tataatcctt tgggtatata 120540cccagtaatg ggatggctgg gtcaaatggt atttctagtt ctagatccct gaggaattgc 120600cacagtgtct tccacaaagg ttgaactagt ttacagtccc accaacagta taaaagtgtt 120660cccatttctc cacaccctct ccagcacctg ttgtttcctg actttttaat gattgccatt 120720gtaactggtg tgagatggta tatcattatg gttttgattt gcatttctct gatggccagt 120780gatgatgagc attttttcat gtgtctgttg gctgcataaa cgtcttcttt tgagaagtgt 120840ctgttcatat ccttcgccca ctttttgatg gggttgtttg tttttttctt gtaaatttgt 120900ttgagttgtt tgtagattct ggatattagc cctttgtcag atgagtagat tgcaaaattt 120960ttttcccatt ctgtaggttg cctgttcact ctgatggtag tttcttttgc tgtgcagaag 121020ctctttagtt taattagatc ccatttgtcc attttggctt ttgttgccat tgcttttggt 121080gttttagaca tgaagtcctt gcccatgctt atgtcctgaa tggtattgcc taggttttcc 121140tctagggttt tgatggtttt aggtctaaca tttaagtctt taatccacct tgaattaatt 121200tttgtataag gtgtaaggaa gggatccagc ttcagctgtc tacctatggc tagccagttt 121260tcccagcacc atttattaaa tagggaatcc tttccccatt gcttgttttt gtcaggtttg 121320tcaaagatca gatggttgta gatgtgtggt gtcatttctg agggctctgt tctgttccat 121380tggtctatat ctctgttttg gtaccagtac catgctgttt tggttactgt agccttgtag 121440tatagtttga agtcaggtag catgatgcct ccagctttgt tcttttggct taggattgtc 121500ttggcaatgc gggctctttt ttggttccat atgaacttta acgtagattt ttccaattct 121560gagaagaaag tcattggtag tttgatgggg atgacattga atctataaat taccttggga 121620gtatggccat ttttatgata ttcattcttc ctatccatga gcatgggatg ttcttccatt 121680tgtttgtgtc ttcctttatt tcgttgagca gtgatttgta gttctccttg aagaggtcct 121740tcacatccct tgtaagttgg attcttaggt attttattct ctttgtagca attgtgaatg 121800ggagttcact cctgatttgg ctctctgttt gtctgttatt ggtgtataag aatgcttgtg 121860attcttgcac attgattttg tatcctgaga ctttgctgaa gttgcctatc agctttaagg 121920agattttggg ctgagacaat ggggttttct aaatatacaa tcatgtcatc tggaaacagg 121980gacaatttga cttcctcttt tcctaattaa atacccttta tttctttctc ctgcctgatt 122040gccctggcca gaacttccaa cactatgttg aataggagtg gtgagagagg gcatccctgt 122100cttgtgccag ttttcaaagg gaatgcttcc agtttttgcc cattcagtat gatattggtt 122160gtgggtttgt cataaatagc tcttattatt ttgagatacg tcccatcaat acctaattta 122220ttgagagttt ttagcatgaa tggttgttga attttgtcaa aggccttttc tgcatctatt 122280gagataatca tgtggttttt gtctttggtt ctgtttatat gctggattac atttattgat 122340ttgtgtatgt tgaaccagct ttgcatccca gggatgatgc ccacttgatc ctggtggata 122400agctttttga tgtgctgctg gattcggttt gccagtattt tattgaggat ttttgcatcg 122460atgttcatca gggatattgg tctaaaattg ttttttttgt gtgtgtatct gccaggcttt 122520ggtatcagga tgatgctggc ctcataaaat gagttaggga ggattccctc tttttctatt 122580gattggaata gtttcagaag gaatggtacc agctcctcct tgtacctctg gtagaattcg 122640gctgtgaatc catctggtcc tgaacttttc tgggttggta ggctgttaat tattgcctca 122700atttcagagc ctgttattgg tctattcagg gattcaactt cttcctggtt tagtcttggg 122760agggtgtatg tgtcgaggaa tttatccatt ttttctagat tttctagtta atttgcttag 122820agttgtttat agtattctct gatggtagtt tgtatttctg tggaatccgt ggtgatgtcc 122880cctttatcac tttttattgc gtctaattga ttcttctctt cttttttttt tctttttttt 122940taattgatca ttcttgggtg tttctcgcag agggggattt ggcagggtca taggacaata 123000gtggagggaa ggtcagcaga taaacaagtc aacaaaggtc tctggttttc ctaggcagag 123060gaccctgcgg ccatccgcag tgtttgtgtc cctgggtact tgagattagg gagtggtgat 123120gactcttaag gagcctgctg ccttcaagca tctgtttaac aaagcacatc ttgcactgcc 123180cttaatccat ttaaccctga gtggacacag cacatgtttc agagagcaca gggttggggg 123240taaggtcata gatcaacagg atcccaaggc agaagaattt ttcttagtac agaacaaaat 123300gaaaagtctc ccatgtctac ctctttctac acagacacag caaccatccg atttctcaat 123360cttttcccca cctttccccc ttttctattc cacaaaacca ccattgtcat catggcccgt 123420tctcaatgag ctgttggcta cacctcccag atggggtggt ggccaggcag aggggctcct 123480cacttcccag taggggtggc caggcagagg cacccctcac ctcccggacg gggcagctgg 123540ccgggcgggg ggctgacccc cccacctccc tctcggacgg ggcggctggc cgggcggggg 123600gctgaacccc tcccctcccg gacggggcgg ctggccgggc ggggggctga ccgcccccca 123660cctccctccc ggacagggtg gctgccgggc ggagacgctc ctcacttccc agacggggcg 123720gctgccgggc ggaggggctc ctcacttctc agaaggggcg gctgccgggc ggaggggctc 123780ctcacttctc agatggaggg gctcctcact tctcagatgg ggcggctgcc gggcggaggg 123840tctcctcact tctcagacag ggcggccggg cagaggcgct cctcacctcc cagacagggt 123900ggtggggcag tggtgctccc cacatctcag atgatgggcg gctgggcaga gacgctcctc 123960acttcatccc agacgatggg cggccaggca gagacgctcc tcacttccta gacaggatgg 124020cggccgggca gagacgctcc tcactttcca gactgggcag ccaggcagag gggctcctta 124080catcccagac gatgggtggc caggcagaga cgctcctcac ttcccagacg gggtggcggc 124140cgggcagagg ctgcaatctc ggcactttgg gaggccaagg caggcggctg ggaggcggag 124200gttgtagccg agccgagatc acgccactgc accccagcct gggcaccatt gagcactgag 124260tgaatgagac tccgtctgct atcccggcac ctctgggagg ccgaggctgg cggatcactc 124320gcggttagga tctggatatt agcctgttct acacagtgaa tctccgtctt taccatttta 124380ttatgttata ttagttctgc gtggtggtgc gcacctgtct ctctcatgca ctcggctatg 124440ctgtagcatg acaatccgtc agtgttgttg tttcgactct tgagtcgcct cgacactcag 124500tcttggtgct ccttatcata tggagatcgt tggtcgtcgt ttcttgtttg ggttttgctt 124560gtgccttttc tgtttattgt atcgcttatg atcgtgatgt tgtcttagtt gcttgttggt 124620ctctagtgtg ttttttgtgt tgtatcttgt tcttgcgttg agtgggttgg gcgttggccc 124680gaggctttct tttggttctg tggtgtctct gacgttttgt tgctctnnnn nnnnnnnnnn 124740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 124800nnnnnnnnnn nnnnnnnnnn nnnnnntaga tttggacgtt atgttttgag tattttattt 124860gattgggtat ggttagtagt attgaggggc atgtgggtta attaggtttt gtatagatta 124920tatttatttt atttattgta ttttaatgat tagatgttgg ttttaacgtg atagatggtg 124980ggtatagatt atagatgtga gtcggtgagc tatatgggat aggtaggttg ggtgtgtcgt 125040gcttctcgcg gcctctctca gcagtgtgct tcggcctcga gaccaccaac tcaacatcac 125100gtccatctca tttcgcgcta cgacacactc atgtctgtac gcacgagtgt atcttctcgc 125160gagcgcttct gtctggcgtg ctgggggggc gtgggggggg ggggcggggc ggggggagat 125220aggacgtgga agaggagaga tggcagaggg gagatggcag agggagagct gaatcttctc 125280ttttcttctt tattagtctt gctagcagtc tatcaatttt gttgatcctt tcaaaaaatc 125340agctcctgga ctcattgatt ttttgaaggg atttttgtgt ctctacctcc ttcagttctg 125400ctctgatctt aattatttct tgccttctgc tagcttttga atgtgtttgc tcttgcttct 125460ctagttcttt taactgtgat gttagggtgt caattttaga tcttccctgc tttctcttgt 125520gggcatgtag tgctataaat ttccctctac acactgcttt aaatgtgtcc cagagattct 125580ggtatgttgt gtctttgttc tcattggttt caaagaaaat ctttatttct gccttcattt 125640cgttatgtac ccagtagtca ttcaggtgca ggttgttcag tgtccatgta gttgagaggt 125700tttgagtgag tttcttaatc ttgagttcta gtttgattgc actgtggtct gagagacagt 125760ttgttatggt ttctgttctt ttacatttgc taaggagtgc tttacttcca actatgtggt 125820caatttcaga ataggtgtga tgcagtgctg agaagaatgt acattctgtt gatttggggt 125880ggagagttct gtagatgtct attagctccg cttgctggag agctgagttc aattcctgga 125940tatccttgtt aactttctgt ctcattgatc tgtctaatgt tgacagtggg gtgttaaagt 126000ctctcattat tattgtgtgg gagtctaggt ctctttgtag gtttttaaga acttgcttaa 126060taaatttggg tgctcctgta ttgggtgcat atacgtttcg gatagttagc tcttcttgtt 126120gaattgatcc ctttaccatt atgtaatggc cttctttgtc tcttttgatc tttgttggtt 126180taaagtctgt tttatcagag actaggattg caacccctgc ctttgttttc catttgcttg 126240gtagatcttc ctccaatccc tttattttga gcttatgtgt gtctccgcac atgagatggg 126300tttcctgaat acagcacact gatgggtctt gactctttat ccaatttgcc agtctgtgtc 126360ttttaattgg agcatttagc ccatttacat ttatggttaa tattgttatg tgtgaatttg 126420atcttgtctt tacgatgtta gatggttatt ttgctcatta gttgatgcag tttcttccta 126480gcattgatgg tctttacaat gtggcatgtt tttgcagtgg ctggtaccag ttgttcattt 126540ccatgtttag tgcttccttc aggagttctt ttagggcagg cctggtggtg acaaaatctc 126600tcagcatttg cttgtctgta aaggatttta tttctccttc acttatgaag cttagtttgg 126660ctggatatga aattctgggt tgaaaattct tttctttaag aatgttgaat attggccccc 126720agtctcttct ggcttgtagg gtttctgccg agagatcagc tgttagtctg atgggcttcc 126780ctttgtgggt aacctgacct ttctctctgg ctgcccttaa catcttttcc ttcatttcaa 126840ctttggtgaa tctgacaatt atgtgtctcg gagttgctct tctcaaggag tatctttgtg 126900gtgttctctg tatttcctga atttgaatgt tggcctgcct tgctagggtg agcaagttct 126960cctgcataat atcctgcaga gtgttttcca acctggttcc attctcccca tcactttcag 127020gtacaccaat cagatgtaga tttggtcttt tcacatagtc ccatatttct tggaggcttc 127080gttcatttct ttttattctt ttttctctaa acttatcttc tcgcttcatt tcattcattt 127140gatcttcagt cactgatacc ctttcttaca gttgatcgaa tcggctactg aagcttgtgc 127200attcgtcaca tagttctcgt gccatgtttt tcagctccat caggtcattt aaggacttct 127260ctacattggt tattctagtt agccattcgt ctaatctttt ttcaaggttt ttagcttttt 127320ttgcgatggg ttccaacttc cttctttagc tgggagaagt ttgatcatct gaagccttct 127380tctctcaact cgtcaaagtc attctccatc cagctttgtt ccaccactcg cgaggagctg 127440ccttcctttg gaaggggaga ggcactctga tttgtagaat gttcagcttt tctgctctgt 127500ttttccccat ctttgtggtt ttatttacct ttggtctttg atgatggtga cgtacagatg 127560gggttttggt ggtgtggatg tcctttctgt ttgttagttt tccttctaac agtcaggacc 127620ctcagctgca ggtttgttga tgtttgctag aggtccactc cagaccctgt ttgcccgagt 127680atcagcagca gaggctgcag aacagcgaat attgctgaac agcaaatgtt gctgcctgat 127740tgctcctctg gaagctttgt ctcagaggtg tacctggcca tgtggggtgt cagtctgccc 127800ctactggagg gtgcctccca gttaggctac tctggggtca gggagccact tgagggggca 127860gtctgtccct tctcagatct caaattccat gttgggtgaa ccactagtca cttcaaagct 127920gtcagacagg gacatttaag tctgcagagg tttctgctgc cttttgttcg gctatgacct 127980gcccccagag gtggagtcta cagaggctgg caggccttct gagctgcgat gggctccacc 128040cagttcgagc ttgctggcaa tgagcaaggc tctgtgggca tgggaccctc cgagccagac 128100atgggatata atctcctggt atgctgtttg ctaagactgt tggaaaagta cagtattagg 128160gtgggagtga cccaattttc caggtgctgt ccgtcaccac ttcccttggc taggaaaggg 128220aattccctga ctccttgcac ttcccaggtg aggtgatgct cacactcaat gggctgcccc 128280cactgttctg cccccactgt ccaacaatcc ccagtgagat gaacccagtg cctcagttgg 128340aaatgcagaa atcacccatc ttctgcattg ctcatgttgg gagctgtaga ctggagctgt 128400tcctattcgg ccatcttgga accagtctga attagtgaat tttcaacata aaactcaact 128460cctgtgctct tagccactca ctttacaaaa gccaaatcca gttcctcatc ctcttgccca 128520ctcactcagc aagccttggg gcccctgcct ttcagtgctt agagaatcat gttcagatgc 128580aagagccagg cttggttctc actgggcttg gcaggcacag tacatgccta actctggcct 128640gcgtgtgcat ttccacttga ggtcctaacc ttccactcag cccctcaaga ctcttcaggg 128700acctaagggc ccctggtgca gagctgcctt tttgctccag gagaaaggca tttcgtggcc 128760cttggtgggt atctttaaat cactgcagaa tagtttactc ctcttctctg aaattcttat 128820actgagcaaa aacgggagtt cactctgggt tttagtgttg cctgtgagag cagcagttct 128880cacagctctt atgtttctaa gtccatttca ttgtttcatg gagaaatgtg tgacgatgac 128940acaacccggt ttcctgtcgt gcctacacag aggattgaga atgagctcca ttctaaaaga 129000gtgcagtgtc ctagatggct ttagaagaga cacatgtgct tggacttggg ctctgtcact 129060gaagctgtgt gaccaagagt tgccctgacc ccctcacatg taaaatggag atgataatgc 129120ctacttctcc gggtcatggt gtgcatttgg ggagagaatg caggaaagcc ctccacacgg 129180cgcctggcac gcagtaaatg ccaatggatg ggaacaatga tcattatcat tatgcctata 129240gtcatcactg gcctgagggt tctgctcctc cctctaagcc tagctctgag agcatcttat 129300ctctcagcct gtcctgctgc cgtctctcac tgcaggagtt ggagcaggta gcaggtagct 129360aagggcgggg ctgcttctca gagcctttag agcagatgag gtcatatcat gaccactctt 129420taaactattg cttaaggcca agcctgtgaa cgcagcccat agagctgtga atagggggag 129480cccagatctc ctgaaaacag gtcctggaat agtttctcta gctctcgtgg taaagcattg 129540tgttttaaaa cttacttttc aacacagatt attatatttt tgtaaaatat aataaaaatt 129600aattactaga aaaatgaaat tttaaaaacc caaagatatt caaaatccaa gtcttttttt 129660ttcttattag gtgcaaaagt cacaaaattg ttctgttaag ttgctataaa agtttctaaa 129720acccctcctc tgagtctttg tattttattt tgtatttatt ttatttttat tttttttaag 129780agacagggtc tccctctgtc tcccaggcta gagtgcagtg gcatgatcat agctccctgc 129840agccttgaac ttcaggactc aagtgattcc ccaacctcag cctcctaagt agttgggact 129900ataggcacac accaccacac ctggctaatt tttttttttt ttttgagaca gagtcttgct 129960ctgttgccca ggctggggta cagtggcatg atctcagctc actgcaagct ccacttccca 130020ggttcacgcc attctcctgc ctcagcctcc caagtagctg ggactacagt tgcccaccac 130080cacatccagc taattttttg tatttttagt agagacgggg ttttactgtg ttagccagga 130140tggtctcgat ctcctgacct tgtgatccac ccgcctcggc ccctagctaa ttttcaattt 130200tttttgtaga gatggggtct ctctacattg cccaggcttg tgacaaaatc ctagactcaa 130260acaatcctcc cgccttagcc acccaaagtg ctggaactat aggcttcaac ctctgtactg 130320atctaattgc agacctggac gcagttagtt ctcagatgac actcaggcca cattctgagc 130380tgtccacccg acagcacttc caggctgaga catcttcatc cttgagcttg gaactggggt 130440ccaaaaagga atattaacgg gatacctctg catcctcccc tggggatgta aagccagcca 130500ggaggggagg cttgaggcca cagaaggcaa gctgagtatt aatggtgaca ataactgtta 130560tcattggcta tgggggtaat tctggaggca ctcaacattc atcatctctt atctacctta 130620aggaaccagc taatctcttg ggcaccttga ctctgaggag ctggatctct tcagtttcac 130680tggtatgaat aagtcagtac atgcggaatt gcaacatgcc aagtttcaaa aagagaatag 130740ttctgtctcc cctggtcccg aaggtgtttg aagaatttat ccaatggctg atgaagttct 130800gggagcacag caggatgaga aagcctggac tgacggcaga agagctcagc agggcaggtt 130860ccaaggactg acacagtgcc ttaatgaagc tgtcccttca cacagatgct ctggatctgt 130920ggggccacag taatggccct ctgacattca tgttcaccca aggtgtagtg gtgaaatatt 130980agatttaagg ggtgatcata tggtgagtaa ttgaatcact tgtaggctgg cccccctcat 131040ttccaggttg ttcaagggat tggttgtttt gccatgtggt ggattaataa ttggccacag 131100ctggacatgg tagctcatga ctgtaatccc agcactttgg gaggccgagc cgggtggatc 131160acctgaggtc aggagttcca gactagcctg gccaacatgg caaaaccctt tctctagtaa 131220aaatacaaaa attagccagg cttggtggct acctggagcc tgtaaaccaa gctccctagg 131280aggctgaggc aggagaatcg cttgaacctg ggaggcagag gttgcagtgg gctgagatcg 131340tgccactgcc ctccagcttg ggcaatagag tgagactctg tcccaaaata ataataataa 131400taataataat aggccacaaa ttctttgaca ttccttccat tgagagatgg gggggtctgc 131460atccccaccc ccacccccgg agtctgggtg ggctctgtga ctgcttggac cagtagaata 131520tgatggaagt tacagtgtgc cagtttccag gcccaagcct gaagagattg gcagcttcca 131580ctttctgtct cttagaaaac ttgctcttgg aagccagcca tcatgtaaga aatgcaggca 131640cccagaggac accattctgt gagaagcctt aaagaatgag actccatatg gacagagagg 131700ccaaggagcc tagaggtgcc agacacatga cagaagaagg cccagccccc ttcagctgat 131760gctacatggc cacagatgaa ctgcccagtg aagcctttcc taaattcctg acctgcaaga 131820gcatgaacaa aattaaatgg ctgtttaaag ttaccaagga ggttggtttt acagcagtag 131880agagaatgga acttgctgga cagcttctct atgctcaccc tccaacctgt gctgtccctg 131940aaagtgattg gaatgagatg cctcaatggg ttttccactg ggaactctga ccagaaagga 132000gggaatgtgt agaggaagga gaggccaatc actccccaga acctttctac tgagggccgc 132060agctcctcca agggtgctgt ctccaggata cttctgcatt actcccacaa ccttccccct 132120ccccctgcat caggactagt ggtgctgatg gccccgcact ctgcatggtc cctctgcaca 132180tgggcccaca cctttgtgat gcccctttgt taaaccctca tcctagtgta ccatctgttt 132240tttgccaaga tgagctgaag ggtctgcagg aagaagggct tcataaccga gaccttgggt 132300agatgtgcag gagggagttt cagagccagc attttctaac taccagaaaa agagaagtgc 132360cctgtccaca accttccaca ttctgttgac cacaggaata aatgaacttt gtgatttttt 132420ttcctgtcat taggtagggg gacaaaaatc ctcttaactc catgttcaga aatattctgg 132480aaatttggtt tcttaagtca aattccacat cagaaacgat gggatgtggc ctggtagctt 132540aagaacagca ggtacagaat aacttttttc tcctttgtct cagttttctt agctataaaa 132600tgggggtaac agtcatctct tcctacctac cccaccaaac ttcgtgaaga acacactagc 132660tacaggaaac ttggcaaaga gaaagagttt gacgctgtca tggcagaagc agtggctttc 132720tcttattacg gtggctgcct ggcctttgaa ggcttatctt tccaggttta aactgagagc 132780aagctgaagg gttatgactc gaagtgaggt aggattttgc caggcacact aggaggctgg 132840gtatgcaagg tggttcattc agggagcctt ggctgacctt gacgcctgcg tccgtctctg 132900atgctctgta ccaggtcagc tgtgtgtccc taacaaagaa tggcctccta actggggtct 132960gcacatctgg gcatcttagt aaggggaaca gatttgaggt tcttctttct ttcagcgaaa 133020ggtgagactt cgggttgaag aactggtaac tgtgatttgc aggcttgtcc atggctcctc 133080agagctggca cttggggtgc tccttgtcca ccccttccct ccctaggccc acatggggca 133140ccctgagctt gcctgagccc tactgtgatc tcacggaggg caggctatga actagaaagt 133200gatgccacac cctggcaact agagaagtgc ccatccaggg gcatgtccca taggtatctg 133260cctgtatgga ccctagttac ccactgacaa aaagaggggc acaaccctcc cctgtagaac 133320tgtagcgcag agtgaggaca tgacgacagt gaggcctggg gccacccgct ccaccctcag 133380gggcttgtaa gaatcccaag gaaagtcatg cctcctgcag gtgtgtcgag gcacagggca 133440gagccctccc acttcccgcc cctgcacagc agtgtcattc ctcctatgac tacagtgctt 133500tgaggacagg aagaactttc ggttcttggg aagcccgggg cagaaggggc ctgagctgct 133560caccgcgtcc atccctcagg acaacctcga catcgccggt gatccagcgg tagcagggga 133620actcgatgta gtccccgtgg ggcgtcttca gcgtgatgta cttcaggtac cagtcgtcat 133680tcagccagta cttgcgcttc tcgattctga ccagctggat ctcgcccagt tcctcgtcca 133740cagtcacgtc gtatgaatcc acctgggaag gagaacaggc aatcaggcca tgcgtggtct 133800cctgagcctt tgttctggag gtgttaagaa tttgtcaccc caaggtatgc tgctgtggca 133860cagggactac gttgaatgaa aagcacttaa aaaaacagca ggtgcaagat actctgacat 133920tcgtgctgtt tcttagaagc aggagatgaa agtcccctat gaaagatgtc ctcactatac 133980caaaaggaaa gtaacactct tttcatcaag gttgggaagt tgaagccaaa ggaaatccat 134040acaaacagat cttgtacaat gaacgcttac ctcccgagtc acttccctgc ccagttacct 134100gccctagccc aagccccttg gccttatcac atctcacgat gattcattca atgcagtatc 134160taagggggat tgactctaac tgcttcttgg ggtcttcatc tttttaagag ggctcccatg 134220ccacgtacaa cttgtattaa agaaatctgt aggctttcgc ccatgaatct gtcttatgcc 134280gattctcaga cctagctgaa atgggaagag gtggagtttt gcctccacta cagttcatgg 134340agccaagatt cttcatgaac accttaaatg aactcctgaa ttcatttacc caagacattc 134400actgaaggcc tgcccctgac aaggctcagt gctacgaccc aggcaggtga aagaaatgga 134460caaatcctgg gccctgccct tgaggagaga gaatccagtg agccaggatg gtgctgtgct 134520gctcagaggg caacgcctgc tgactgcact tagctcaggc tggaggatgg ggagatgccc 134580aggctcatgc ctgcacctgc gaggctgtgc ccagtgaggg tccgcagctg cccccacatg 134640ggatagcctt gtgggagacc tttacaactg gagatgggct tgctgacagt ggatccccac 134700aatgactctg ggagtgcagg ggagttacca ccccttccac agatgaagac agcgaggctc 134760agtgactccc acctctgcca cttgccagca gagctggagt cacaccccag gtctttccat 134820catagccact tcctctcttt ccagttttca gtggggataa taagtgagtg aaaatggtga 134880ggcttagata cagggagctt gcatggattc gagtcgtcct gggacatttg cagttcacag 134940ctgcatggtg atgcccagag aacagtgaga ccaagcagct ctgctgcacc tgtggcatta 135000ctggtggcag ggtgaggcta gccttgcagc tagaaggcac caccccaggc tgtgtacagc 135060gcagctgccc aggcctctgg gacaaggctg cttggccccc tccctgccag gctcctgctt 135120ccaggacaca ggcggctctg aggcaacagc catagcttct ggagggcttt tgtctctgca 135180gcaatggatg gcagacagga agttgggatg agaggctcag gcattgcatc acgcattctg 135240atgaccagca ggtttggaaa gccccggact cgatgccctc caaggcatgt tcttttttat 135300tttgaggtgg agtctcactg tgttgctcag gctggagtgc agtggctcga tctcggctca 135360ctgcaacctc cacttccagg gttcaagtga ttctcctgcc tcagtctccc aagtagctgg 135420gactacaggc acctgccacc acatctggtt aagttttgta ttttcagtag agacagggtt 135480tcaccatgtt ggccaggctg gtctcaaact cctgacctca agtgatccac ctgcctcggt 135540ctcccaaagt gctgggatta caggcatgag ccactgtgcc tggcccctgc aaggcacttt 135600ctaagccaga ttcatcaacc cttgaacttt atccctggcg gcagaaccta gagttctcag 135660catgatggca aagtggaagg ggcgaggggt atcatccttt gtgcgagcgc aggcatcccc 135720attccatgcc ctgcctccaa gctgccagga ggacccctgg agggcttcct aagaggaatg 135780ggaacttgag tgttttccac aaacctcctg gctctgaata ccgtgagcag tttggggtgg 135840ctgacgctga ggccagacgc ttgtggtcag aaagcggaag cttcctccct gtgctctgta 135900gtctaggcca gaggccctag gaacagtttc tgatgacaga ggagccaggc ccatgtggtg 135960ggatgacatg tcttgggtcc agccacatgt ggaccagcct cggccccacg acaatgcccc 136020tcctgcaggg agagtatcag cctcatctgc cacctctgag aaagccttcc tgccacagct 136080tgtcctcagg agagcagggc cacacacaag gcctcgccca tcatggacag ggtgggcagg 136140ttctgatggg acaggagtct cagcatctca ggggctcggg cccatctgga ggagcagagt 136200gggggctgag tggcccagga ctggatacgc gtcgggcctg ccccacgcca ggccctgcat 136260gccacaggga tttacagagg ccttagctgc aggttgtgag ccaagacaga gattcctgat 136320cccaccgagg agtggggaga gggcagtgag gggtagaagg ctcggtgggc cctggggtct 136380ggagctctga attcacatcc cagcttcagg caagtagctg gatggcctgg gcacattgct 136440tcattctctg aagctgtttc ttcatctacc caaaggagat acccctcact tgcaggacca 136500ttgagaaaat gaaatgacac catcctgggt ggcctccttt ctccagaggc acaggccacc 136560ggttgtgtgc atcagaacca ccagtgcccc ttcctgaatc cagggggttg cagggcacca 136620ggccccacac cgcctgtgag ctagcatggg cccctgcgtc acggccactt cgtgactaca 136680tgtgccaatc cgagtaactg gagcacacat gggaggagga aactgaacct gtgacctcaa 136740actccttcac tcaaagccca gaaggaactc ctgaaaggct ggagacaggg ctgtgaggac 136800agagggagag tgggaggcat tctgaatgct ggtggggcag gcagagcaag tctgtgtgcg 136860gtgaggactg aggggcggcc accagaggcc cgggcctggt ctgagtgtgg ctgctgcagg 136920ggaagtagct ttcctatggc tctgaggggc tggagaggga ggaaggtgtc cccaggggat 136980agatgacagg aaagctgggg tggggtggga ggagctaaca gggacactct catgggaggg 137040aggctcccag taccttggac taagagggga gggagatggg ctgaggaggc attccaggac 137100tgggcaggtc ccacagggag ggcccgatgt ctacctgggg gtgacgaccc acacgaagag 137160actgagcagc ttctctgagg gagtggacag tggggtgacc ctccagcaca cctgtggtgc 137220agtcacaggg gactgggaca ctcctggccc cctgccctac ctacacctgc aggcacctgg 137280tggagagccc ctgagtttca cttggggtga aatcactagg cagatttaca aggactccat 137340ctgccatctg acagggactt ccacagcagg agggggccag gcctgcagtc tggctggtct 137400atggccagcc cagccacttt cctggtgctg gcttcaagcc tgaatatgat aagaggtgaa 137460caaggggcag agggctccag ccttgaccca actcagggag taaaagacaa agcttgatgg 137520ctgtggttct aacatcccag gggccactaa tggaagaatg acagataaag gcctccttcc 137580aggaaaatac cagggtcaca tgtgtcaaac tgcaaaatat cttcggaagg tttgtggaac 137640tacaatggcc accttcctcc ctcctctctg ggccaagtac actgggctga tgctatattt 137700gtctatttgc tgtgacacat taaattctca ggtgacacac tgaattatgg tgggcactat 137760caacagtgga caccccccaa caggccctca ggaagcactg ggcctgggaa tggccgagcg 137820agtgattatc ctgctttaca gatagaaact gaggctcaga gagcaatggt ggcctcaaga 137880tcagaaccta cacctgtgtg atggccaagc ccaggttcct cccacagttc cccaaggcca 137940catcttgaga aaagccctga accggaagct agaaggccca gctttgagcc ctggtttgta 138000ccctggatgg agtgtaaagg ggcaatgtgg agtggcatgg aaggacaggg aaaagcttca 138060taagagggta tttcaggcag cttttagggg ctgatcagat ttcagagggc agatgagtag 138120gaggatgggc atgggtagca catttcagat gtggggaact gggagggcag ggtccccggg 138180ggattcacac gtgggaggag gaaggctgga cacagtttct ggggacagaa gtgggtggcg 138240tggtggtgca gatgctggag agaaacaagg gaaacctgca actcaccggc ctcatttcac 138300aaggggccac tttgctgatt ttgcttgttg cttttaactt tttattggat atgtagtttt 138360aaagcttttg aatatttcgg caaagagtca gttggtcagt tttcatgtga gctttctatc 138420agcattttag gaaagttttc atctgtcttt catcagatct ctgctgttgc tgggacaggg 138480agacggatct ctctgcggaa gcaaggatgt gtgccccacc cccagactac ccatcttgga 138540gggggactaa tgaggggctg tgggaggagg aagctgtaga gcagagagga agattggggg 138600ccagacaaaa cagagactcg ggatgagaga gggggaggct atggggaaga gctgaggaaa 138660acaagggacg gagagaggga ttggggtagt ggatagggag accaaggggc agagaagggg 138720gaaaatgtgg gacagagagc cggagattga gggagcagag aaggggcctc tgagaggtgg 138780ctggaagaca gagtgtagag gaaaattcag agcaaaaaag aagggagagt tgtagggtag 138840agagcagaaa cggggggcat ttagggaact gggagcaggg gaaggaagag gaggcagaag 138900agggggcaga atgtagggaa aagatggctc agagggggag actgggagat gagagacgga 138960ttgagggtca taccaaagaa atatttatgc tccactaata tattcccatt cacaagaagg 139020aggtattcta gaattaattt ttcatgaatg taagaatact cactgactta agaagcatga 139080atcattataa cacttttcta cacttgttga acatattcaa gtatcatata ttaaacacca 139140cgctattgcc cttagctttc ttaaaagtac cagcacagaa tgaaatgggc caactaagat 139200ttctcagaaa tcttcagatc tgttacagtt atcgtatccg atctgaatta ctttaaattt 139260acagagttaa agaaataaat caacttgtta aatttgccta gacttgggtt agcctctgga 139320aacagggcca ttctgtgtag gatcccggct ttctccagca gacagtgagg agccaaggaa 139380gacacaaccg tttataggat aatcccacct gcgaaggact tgattcccag tcctgactga 139440atcactatgc ggaactctga tgagaataac aagggacctg tgactcactt tatcaaggaa 139500aaaatcagtc ctgtccatta tttgactgaa ctatgccctg gaggaggggg gtgttgacct 139560gccaataact tggaaatgat taaattgtgt ggaaatacct ttattgttat tgttatcgtt 139620actactgtta acaatacaga ttttaacact tttccctgta aaatgggata accttgtctt 139680ctcagcaagt acttcctcta gggtttctct tttaccttta cccaaaaagg atgattgtct 139740ttacagatac caagctggga gaatgaggaa gtagttctgg gctgggcaag agaaagtgtt 139800gagtaacttt gcatcaacag gaactttctc aaaagctgat tttttcaagg aaactttcag 139860ccttagctgg ttccccaccc tgcggtagcc cccaccccca accacccccc gccccacccc 139920tgacttatag ccctctcaac ccaatggaaa tgtgacatgc aaatctctcc tgaaatttgc 139980ccctgggatt ccggctgagg tgtttaatta taatgtcatt tgaatgactt ctcagggtag 140040gggaagacag accgagagag ggagatagtg gacaaaacag tgccttcact ctttgtccga 140100agagcccgct atggccacgt tatcatgtcc cctgcaggag gaaagcacac acggggtgcc 140160cgtgctgtgc agtgtgagca caccaatggc ctgtgcttct agaaccttat gatgtgtgga 140220ggagaatggg gaggaggaag cacttacaaa ggggaagtag cctgtaccct gtccttctcc 140280cccaggtgtg gggaatccat gaaataatgg aagtaaatga aggtgcgtca gaaatggcag 140340agggtgccat caccctaaat ggacgacaca ttaactccag ggcaccgcag tcctcagggt 140400cagttccccg actggggcaa ctaagcaact gaaggttgct cttgtactca acacctaact 140460cttcttgaag ggagctcaag ccaggatgct agtgaacttg gctgagggcc atgtcgtgct 140520acagcgaggg tatgggctcc tggataggaa tcgagcccat ggagagcttg gaatccagaa 140580aaagagtagt ttttacttac tttaggctct gcacacctaa aacacaaatt ttatccaaga 140640gtaatcagtt gaaacatgaa cttgccggtt gtatttgtcc attatgagca atttcacaag 140700gtttgatcaa ataataaact aaatggtgag aaggcagctt ggtaaagtct aaatcttttt 140760ggtccagtta ggagctgggg tggggtggaa gggaactgat attccctgcg cacctgcagt 140820atgtctggga ctattaccag tcatttacgg agcgcttcct ctgtgccagg tactggatta 140880ggcacttcat taaaggctca gtgagaactg cgacctgccc atgctcatgg aaaagagtcg 140940tggggctgga attcaggtcc agatcagcat taggaactag cttccaaatg ctgagggctc 141000cctcgcgtcc tcacacctag cgtggaagtg ttccctggtg tacattctgc tgggagggag 141060tgcagcccct ccatttagga aaagcctctc ctggcctggg ttctgttcct tccctttaat 141120ttcctctggg gactgtcctg agaatgtaag ctgcccaggt gccgtctgag ccaccagatg 141180ggcctgggct cacccatgac tcatggacca gactgaagcc aaccctccat tttcagctgg 141240ggaaactgag gcccagacag acggtagagt gagctggtgg tcatgtctgg acagaactca 141300ggcctctgga cccgctgacg tgcacccttc cccacggctc acagctggct gctccgaggt 141360gccctcagag ttctgtgagc atgactcatc tgcctctccc tccctgtgcg acctgcagaa 141420ggagcctaag gaagaggtgg cacaagaaac gcggcccagt gaagggggct ccggagcctc 141480ggagcccaga tcttgaagtg gaggggaaac cttgggcctt ctcctcccag ggaagacgcc 141540ttgcaggcgg cacgcgggaa gtctccgttt ccgacagggg tcgcggccgc cgcgaggggt 141600cctggacgct tgcccaccag ggtacccacc ggccactggg agcccagggc acgccccacc 141660gcagttctgc cctccctgac agggtcctgg acacccccag tcctgtccgg gccgccccga 141720cgggccctcg gcgcccccgc ccaggcctct gccgtccaaa ccgggtcccg gacgcacctc 141780agcccgcgct ccacccgtgc cccgcccgcg ctcaccgcgc cacgctcgaa gtcgttgtag 141840aagggcttgt ccagcaggtg cttctcgctg cagcccgccg agcccacgag gctgaggtag 141900atgtagtcgt cagtgtcttc accacggggt gaagtacggt ccnnnnnnnn nnnnnnnnnn 141960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142020nnnnnnnnnn nnnnnnnnnn nntttttggg ttgttgttgt ctctttgttt ttgcgagcgg 142080tccttccgcc ccgtggtgaa gacaagtggg cgcctctgcc tggccatccc atctgggagg 142140tgaggagtgc ctctgcccgg ccaccccgtc tgggaagtga ggagcgtgtc tacccagcca 142200ccacgtctgg gaggtgagga gcgcttctgc ctggccgccc catctgggaa gtgaggagcg 142260cctctatctg gccacccctt ctgggaagtg aggagtgcct ctgcccggcc gccaccccgc 142320ctcggaggcg aggagcacct ctgcccggcc accaccccat ctgagaggca aggaccacct 142380ctgcctggcc gccatcctgt ctgggaggcg aggagcacct ctgcccagcc gccgccccac 142440ctaggaggtg aggagcgcct ctggccagcc gccccacctg ggaggcgagg agcgcctctg 142500cctggccgcc ccatctggga ggtgaggagc gcctctgccc agccaccacc ccatctggga 142560agtgaggagc gtctctgcca ggccgccccg tctgggaagt gtacccaaca gctccgaaga 142620gacagcgacc attgagaacg ggccatgatg acgatggcgg ttttgtcaaa aagaaaaggg 142680ggaaatgtgg ggaaaagaaa gagagatcag attgttactg tgtctgtgta gaaagaaggt 142740gacataggag acaccatttt gttctgtact aagaaaaatt cttctgcctt gggatgctgt 142800taatgtacaa ccttaccccc aaccccgtgc tcgctgaaac atgtgctgtg tcaactcagg 142860gttaaatgga ttaagggtgg tgcaagatgt gctttgttaa acagatgctt gaaggcagca 142920cgctcattaa gagtcatcac cactccctaa tctcaagtac ccagggacac aaacactgct 142980gaaggctgca gggacctctg cctaggaaaa ccagagacct ttgttcacct gtttatctgc 143040tgaccttctc tccactatta tactatgact ctgccacatc cccctctctg agaaacaccc 143100aagaatgatc aataaatact aaaaaaacaa attaattaat taaataaatt tcatatggaa 143160ccaaaaaaga gcctgcattg ccaagtcaat cttaagccaa aggaacaaag ctggaggcat 143220cacactacct gacttcaaac tatactacaa ggctacagta accaaaacag cacggtactg 143280gtaccaaaac agaggtatag accaatggaa cagaacagag ccttcagtaa taatgccaca 143340tatgtacaac tatctgatct ttgacaaacc tgacaaaaac aagaaatggg gaaaggattc 143400cctatttcat aaatggtgtt gggaaaactg gctagccaat aaatggtgta gggaaaactg 143460gctagccata tgtagaaagc tgaaactgga tcccttcctt acatcttata caaaaattaa 143520ttcaagatgg attaaagact tacatgttag acctaaaacc ataaaaaccc tagaagaaaa 143580cctaggcaat accattcagg acataggcat gggcaaggac ttcatgccta aaacaccaaa 143640agcaatggca atgaaagcca aaattgacaa atgggatcta attaaactaa agagcttctg 143700cacagcaaaa gaaactacca tcagagtgaa caggcaaccc acagaatggg agaaaatttt 143760tgcaatctac tcatctgaca aagggctaat atccagactc tacaatgaac tcaaacaaat 143820ttacaagaaa aaaacaaaca accccatcaa aatgtgggcc aaggaaaaga acagacactt 143880ctcaaaagaa ggcatttatg cagccaaaag acacatgaaa aaatgctcat catcactggc 143940catcagagaa atgcaaatca aaaccacaat gagataccat ctcacaccag ttagaatggt 144000gatcgttaaa aagtcaggaa acaacaggtg ctggagagga tatggagaaa taggaatact 144060tttacactgt tggtgggact gtaaactagt tcaaccattg tggaagtcag tgtggcgatt 144120cctcagggat ctagaactag aaataccatt tgacccagca atcccattac tgggtatata 144180cccaaagtat tataaatcat gctgctataa agacacatgc acacatatgt ttattgtggc 144240actattcaca agagcaaaga cttggaacca acccaaatgt ccaacaatga tagactggat 144300taagaaaatg tggcacttat acaccatgga ataccaagca gccataaaaa atgatgagtt 144360catgtccttt gtagggacat ggatgaagct agaaaccatc attctcagca aactattgca 144420aggacaaaaa accaaacact gcatgttctc actcataggt gggaattgaa caatgagaac 144480acatggacac aggaagggga acatcacaca ctggagcctg ctgtggggtg gggggttggg 144540ggagggatag cattaggaga tatacctaat gctaaatgac gagttaatgg gtgcagcaca 144600ccaacatggc acatgtatac atatgtaact aacctgcatg ttgtgcacat gtaccctaaa 144660acctaaagta taataataaa aaaataagct tataaaatgc ttttaatgct atataacttt 144720ataactctga aagaataata gatttgtatt atgctgtcag tgagtaggtc tattaagtga 144780taacattaaa cacaaatacc accatttata aagtatgatt ttgatatcat cggttgtgta 144840ttttcacccc aaactctgta gatagtaact ttgttttctc atttgaccat gctcaatctt 144900tagagaccat gctcttctct caaaatgact gtaggttcat ccaagtatgt ttgctaccca 144960aaatcaatga aacacagtca caactatgtc tgtctaggaa taaagccagc taattagatt 145020cttattctaa ggaacaagaa gagggcaggt aagaatttga ttatggcaga attctgacca 145080taatcaataa ctgttttcta ttttttctcc atgttgtcgg gtggttcata cctgactttc 145140atcatttcca atgattcctg tttgttttgt ttataaattt caaaatcatc atgaaaagtt 145200tcactgaatg aaaacctgaa aattgggaaa taaacatttt tctgcaaaaa aaaaaaaaca 145260atgaacactg acttctccta tttctcaaag tcatctcaaa gccatatgag gattaccaca 145320aacttgcagg tgagaaaatt ctttgcaatg taagaagtgc catgcaaatt agttgatgct 145380attataatta gtctcaggga tcaaaaatgc ttgaacccta aatctaacac ttcaaaggaa 145440agcattattt ttcattcatt ttacaagtta cacctccctt cttccccaca tctttgttta 145500gaatctggac cctgaagtta gaggaccaag attcaaatcc cagcctcatc agtactagct 145560atgtgatatt gggaatgtta cttgatccct gcttatctta ctttctgcat ttattaaatg 145620gggatgagaa taatatctac ccaaataagg tgcccaatgc agtgcccaac agcatgaagc 145680ccaataaagg ttagttaaca tcacatccat aattgacacc tttaggtatt agattttcat 145740catcccagaa cattttcctt ctttaagcct ttttctttct aagtggcact ttcttggata 145800ctgattgcac tcctcacaaa cttgaaacgt tcaaacacac aagatgccat ttaggagaca 145860ttggaacttg ggtttccttc tctgtaaaac tgagtaatga cagtcacctc ccagggctgt 145920tgtaagaatg aatgagctag agtatgtaaa atatcttagc acagagtgct taagaaatta 145980ttagttccag ccaagcgtgg tggctcacgc ctgtaatccc agcacttagg gaggccaagg 146040caggcggctc atctggggtc aggagttcaa gaccacctgg tcaacatggt gaaaccccgt 146100ctctactaaa aatacaaaaa ttagccaggc gtggtggcat gtgcatgtaa tcccagctac 146160tcaggaggct gagacaggag aatcacttga acccaggagg tggaggttgc agtgagccga 146220gatcgtgcca ttgcactcca gcctgggcaa caagagtgaa actctgtctc aaaaaaaaaa 146280aaaaaaaaaa gaagaagaag aagaaagaag gaaagaaaga gagagagaga aagaaagaaa 146340gaaagaaaga aagaaagaaa gaaagaaaga aaaagaaaga aagaaaagaa agaaagaagg 146400aaagagacag aaagaaagaa gggaagaaag aaagaaagaa agaaagaaag aaagaaagaa 146460agaaagaaag aaagaaagaa gggaagaaag aaagaaagaa agaaaaaaag aaagaaagaa 146520agaaaaagaa agaaagagag aaagagaaag aaagaaagaa agaaaggaga gacagaacga 146580aggaaagaag gaaggaatta tttccctttt tcccacccac gcctcaccac aaaccttgtt 146640gtggggataa catgtaaaag tgaacaaaac tgagaagatc acatcgcata tgcactcagc 146700ctctagtggg agacacaggc aataaacagg aaaagttaaa aacagtacta ggtagtggtg 146760agtgcaatta gcctcccgca gcataaaggg aggagaataa ccagtggctc tttcatgcag 146820agaatcaggg tagacctctg gggagagacc cgcactcaga ctgggatgga attatttatt 146880ttatgcgtgt tcagttagga gtagaggtgc tctgtcgtga ttatctaatc ttgggaggaa 146940caatgtcaga cgggtttcaa gatgagtatg agaattggtc ttccttctga aaaaacaaga 147000attgagttca cactaatcac aaatacatca agcctatttg agtcttgtgt gtgtcatgga 147060ttgaagtgta tctttccact tagtcaagaa gttccaatga gaagaatgct ccttaacttc 147120ctaagtgcaa cagagtagtt gaactgagcc atggatggta aatctactta agaaagcaca 147180tgccatggaa agatagcatt ggctgccctg cctacttctg atggcacagc cttgcaggaa 147240gggaaagaag ccaagaggca aaagcctggt cttgttctga aaggcacaag gaggtggaag 147300attaggggaa actcacccaa aaatgactca gctctgaact cagactcaag aagccacaat 147360aagcaacatg aatggaactg gagggaaata aaccaggcgt agaaacacaa atgttgcata 147420ttcttactca tgtgggaact aaaaacatgc atttcaagga gaatagaatg atggttatca 147480gagactggga acagttgtgg gtgggggtgt taagagaggt tgcttaatgg gtacaaacat 147540atagttagat agaaggtata agttctaatg tttgacagca gagtaggaga actatagtta 147600acagtaatat attgtatact ccaaaatagc tagacaggaa gatttgaaat gttcccaaca 147660catagaaatg acaaaagctt gaggtaatgg ataccctaaa tatcctgact tgatcattac 147720acagtctatg cgtgtaacga aataccatat gtaccccata aatatgtaca aatattatgt 147780atcaataaaa atttttaaaa agaagctgca atcaacattt catcattatc ttttcagcag 147840catccttaca aagttacaga gacatcttaa gatgtatccc tcttcctctg aatcttcctg 147900ggatagaaca aatgaaaact aaacaaggag tgggctggga ggagctgtca acatccatcc 147960taaggaaaag tctgttaggg ctatgagctt cctctgtcct agagtagagc tgggaaaata 148020gagtcttgac aattgatttc ttattctttc tttctctaaa tccccaatga atatgttttt 148080ctttttagtg ctcagtggta gacagggctt aattctgttt agttatgcaa acaggccaga 148140agaaactgaa ataggctata aatgtttgaa gctggccaaa agaagatgta gtcacacaag 148200ataccctctg gaggaaagtg actgtcagga aagtcacttt cctgaccagc aaaaggacca 148260gcactatttt gtcctgctga agaagtacat ttgtcctgct gctgtcatgg ggacagtggc 148320tccagggctt cctgtggaca tgccacaagt tccactccat gagtcaggag atcatgcggt 148380cagctggggg gaacttaggg ttgctctctt gagcttagcc ttatgctgcg tgtctgcttt 148440gccaaccaca aaaacgatag tttcatagca agttgcaggc tccctggaaa cttaacctac 148500ctactaggat acatttacag ctgcttttct cctggcatca ggctggagat caagaaatac 148560ataaaacata gtccctgccc tcagttagct cagaatatgg tgggggaatg gagacagaag 148620caaatactgg tgggacaagt gacgatgggc caaaatatgg gcttaaacaa aggttgtggg 148680agctcagaaa aggagcaact cactctgcct gaaagagtca tggagggctc cacggaaaaa 148740gcaacatttg tgcaggagga gattagaaca cagaggagaa ggatagcagc actccagatg 148800aagggaagga aatgtgccaa gatttcatgg gaatgagaag atgtaaactg ctcaggaaaa 148860accatggttg aagctcttca cagtggctga agcatggtgt gattgtcagt tttgcttgtc 148920aacttggtca ggctgtagta ctcaactatt caaccaagca ctgatctagg tattgctgtg 148980aaggtatttt gtagtcgcaa ttaacctcta caatcagttg actctattta aaggacatta 149040ttctgtacta tctgagtgga cctgatccac ttcatcccaa aggtaaatga ttctccaaca 149100gtgttctcat ctacatctag ggcttggaag atgaaggtga aggagaatat ggaaaaacaa 149160caagaaaaaa gtcaatcaat cagaacttcc agccctgtgt ttgttagttc actttatgtt 149220caaccagtat gtctccactt ttatctgctt ccttgtaaga tttcatctga aaaagggaat 149280tcactcttca ggtgttggaa atcaattatg agagaagcac ttacagaatg actatctgaa 149340gagctctgca gacattctcc agagtaaaac aaccataacc ggtgaaaatt aaaaacaaac 149400aaaataaaaa aatgttagag tattggaaat tgttctaagg gccgacagca aatgaagaaa 149460tccaagaaag cctaccaatt tcagtgagag gagtgagagt ctatggtgtt gagtctatgg 149520tgtttaagtt ctgttcattt cctccctctc ctgcaatcag ttcagtgaaa cagaaaccct 149580gctccagaga tgtgcagcca aaaagacagg gtgccctttc ccctcagctc ccaatctagg 149640gcagactacc agcatttccc aagcccatct ctggctctat gttgcagaag ttctattcca 149700agaaagtttg actgagaatc tgaagcttcc ttcttccact cagcctctac tattaaggcg 149760taagttctat ccccagtgcc acgggccaag aatactgatg ctgaatgtcc ttgctccgct 149820acactcatag ggcagagatt ccatgggagg agtgaagttt ccattccaga aaaggcagaa 149880aaacagaaat tactgcccaa tccagtaccc cacccataaa gaaggaaaga catctgagaa 149940aagcatgaca ctgtccccaa aaccacctgc agagtagtgg cagtagaggc agtagggaga 150000ctagaggacc tgcctagagg gagaagtagg ccataagaag aaaagtctct agttctccct 150060aaagggacaa actttatttg caacagaaca tgcggaagtt caagcctaag ggcactgtca 150120aaaacaatga atattttggt ggtaagcaat taataggtta atggcagctt agtgagagca 150180acaaactaaa ccataaacca gctagacata tacaataaaa atccaagaaa gagagagcca 150240agaagggcct tcctgaggtt aaaacaaaca tcaaagtctt gtctgaaaaa ctgctcctgc 150300aaaggaagca aaatatattt ggatcagact gtggagcaat ttatgcccct aaaaagctgt 150360caaaaccaaa agagcaatta tctagcaatt gctggaggtt aacagttggg tatgatacca 150420aaagaggcaa acaactgaac agatcagaga aagaggcagt caaagagagc actgctaaat 150480gcactgtaat tccaatttga gagaaaatgt gcccagtgct gtgactccct caggaacaac 150540atcagaggcc acaaattatg gtggaaatag gcttcactat atagtctagc caggcaatta 150600aacaaataaa caattaaaca acagtaacat agtgaggggg gcagaaatga gtattgcaac 150660tagctagaac atatttttga acaaaaaaat tatgagtcaa gcagagatat ggaaaagaca 150720gagccataca ccagaaaaaa ggtaagcaag agaaactgcc tttgtgagtg ccaagatgtc 150780agacatagca gaaaaaaaac ctcaaagtaa ttattataaa tatgttcaaa aactaaaaga 150840aaatgtgctt aaaaaataaa agatggtatg ataatttctt gtcaattcaa aaataccaat 150900aatagattaa aaatattttt tcaaaagtta attaaccaaa tggaaattct ggggtttaaa 150960agtaaaataa caacaataag aacattttgc tagaggaaat gaacaataga tttgtaccag 151020aaggaaaaat aatcaaataa acagggattt catcaacacc aaacctgtct tacaagaaat 151080actaaaggga gttcaatctg aaagaaagaa cattaacaag caacaggaaa tcatctgaag 151140gtacaaaact cactgtacct tcagatgata ataagtcctc aaaaaacaca gaatattata 151200acactgtaat tatggtgtgt aagctactta tatcataagt agaaagagga aaagatgaac 151260tgattaaaaa acaattacaa cagcttttca agacagtata ataagatata taaataacag 151320aaagcttaaa agcatataaa taaagttaaa atgtagaggt ttttttagtt ttctttttgc 151380ttgattgtgt atttatttat gcaatcagtg ttgttttcat cagcataaaa taatgggtta 151440taagatagta tttgcaagcc tcatagtaac ctcaaattta aaaacataca atgtatacac 151500aaaaagtgaa gagcaagaaa ttaaaacata ccaccaaaaa aaatactttc actaaaaagg 151560agacaggaag taagaaaaga tgggagagaa gatcacaaaa caaccagaaa ataaataaca 151620aaatggcaag caagtcctta tttgtcaata ataatattga atgtaaatgg actatactct 151680ccagtaaaaa gatacagagt ggttgaatgg attaaaaaaa 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gagaacttcc ccaatctagc aaggcaggcc 157560aacattcaga ttcaggaaat acagagaacg ccacaaagat actcctcgag aagagcaaca 157620ccaagacaca taattgtcag attcaccaaa gttgaaatga agggaacaat gttaagggca 157680gccagagaga aaggtcgggt taccctcaaa gggaagccca tcagactaac agcggatctc 157740tcggcagaaa ctctacaagc cagaagagag tggtggccaa tattcaacat tcttaaggaa 157800aagaattttc aaaccagaat ttcatataca gccaaactaa gcttcataag tgaaggagaa 157860ataaaatcct ttacagacaa gcaaatgctg agaaattttg tcaccaccag gcctgcccta 157920aaagagctcc tgaaggaagt gctaaacatg gaaaggaaca accggtacca gccgctgcaa 157980aatcatgcca aaatgtaaag accatcgagg ctaggaagaa actgcatcaa ctaacgagca 158040aaacaacctg ctaacatcat aatgacagga tcaaattcac acataacaat attaacttta 158100catgtaaatg gactaaatgc tgcaattaaa agacacagac tggcaaattg cataaagatt 158160caagacccat cagtgtgctg tattcaggaa acccatctca cgggcagaga cacacatagg 158220ctcaaaataa agggatggag gaagacctac caagcaaatg gaaaacaaaa aaaaggcagg 158280ggttgcaatc ctagtctctg ataaaacaga ctttaaacca acacagatca aaagagacaa 158340agaaggccat tacttaatgg 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cctatttaat 160860aaatggtgct gggaaaactg gctagccata tgtagaaaga tgaaactgga tcccttcctt 160920acaccttata caaaaattaa ttcaagatgg attaaagact taaatgtcag acctaaaacc 160980ataaaaaccc tagaagaaaa cctaggcatt accattcagg acataggcat gggcaaggac 161040ttcatgtcta aaacaccaaa agcaatggca acaaaagcca aaattgacaa atgggatcta 161100attaaactaa agagcttctg cacagcaaaa gaaactacca tcagagtgaa taggcaacct 161160acagaatggg agaaaatttt tgcaatctac tcatctgaca aagggctaat attcagaatc 161220tacaaagaac ttaaacaaat gttcaagaaa aaaacaaccc catcaaaaag tgggcaaaat 161280acatgaaaag acacttctca aaagaagaca tttatgcagc caacagacac atgaaaaaat 161340gctcatcatc actggccatc agagaaatgc aaatcaaaac cacaatgaga taccatctca 161400caccagttag aatgacgatc attaaagtca ggaaacaaca gatgctggag aggacatgga 161460gaaataggaa tgcttttaca ctgttggtgg gggtgtaaat tagttcaatc attgttgaag 161520acagtgtggc aattcctcaa ggatctagaa ctagaaatac catttgaccc agcaatccca 161580ttactgggta tataccgaaa agattacaaa tggtgcaact ataaagacac atgcacacga 161640atgtttattg tggcactatt cacaatagca aagacttcta 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cccaaaaaaa aagaaacaca taaaattgaa 162540atgaagaaaa caatacaaaa gatcaacaaa aatgaaaggt tggcttttta aagagataaa 162600caaaattgac aaatgtttag ccagactaag aaaaagagaa ggcccaaata aataaaatca 162660gagatgaaaa agcagacatt ataactgata ctgcggaaat tcaaaggatc attaatggct 162720actatgagca actgtatgcc aaacattgga aaatattgaa gaaatagata aattcgtaga 162780cacacgcaac ctaacaagat tcaaccatga agaaattcaa aatctgaaca gaccaaaaac 162840aagtaacaag atcaaagcca taattaaaat tttcccagca aagaaaagcc tgagacccaa 162900tggcttcact gctgaattct atcaaacatt taaagaacta ataccaatcc tactcaaact 162960attccaaaaa gtagaagagg agggaatatt tccaaactta ttctatgagg ccattactgc 163020tcctatatca aaaccaagga cacatcaaaa aaagaaaact ataggccaat atctcacatg 163080aatattgatg aatcctcaaa aaatgctagc aaactgaatt caacatcaca ttaaaaatca 163140ttcattatga ccaaatggga tttatcccag ggatgcaaat atggttcaac atatacacat 163200cagtcagtgt aacatatcat atcaacagaa tgaagaagaa aaaccatatg gtcatgttaa 163260ttgatgctga aaaagtattt gagagaatta aacatctctt catgacaaaa accctcaaaa 163320acactggaga cagaaggaac atacctcaac acaataaaca gacatatatg acagactcac 163380agctagaatc atactaaatg gggagaaact gaaagtcatt cctctaagat ctggaacaag 163440acaaggatgc tcattttcac cagtgttagt ggaacatagt actgaaagtc ctagctagaa 163500cagactagag acagaaataa ggggcatcca aactgggaag agagaagtca aattaccttt 163560gttcgcagat ggtatgatct tctgtttgga aaaacctaga ctccataaaa aaatgattag 163620aactgataaa ttcagtaaag tttcaggata caaaattaac atacaaaaat cagtagcatt 163680tctatatgtc aatagcaaac aatctgaaaa tgaaatcaag gaagtaatcc catttcccat 163740ttgcaatagc tataaataaa attaatataa tctcatctct tcaataagtg gtgttgggaa 163800aactgcatat ccacatacaa aagaataaaa ttagaccctt ttaatataca aaactggacc 163860caagttaata tacaaaaatt aacttgaaat gaattaaaga cttaaatgta agatctgaaa 163920ccaaaaactc ctagagagaa acatagggga aaagctcctt gacgttggcc ttggcaataa 163980ttttttggga tattacacca aaagcacaga cttttgaaaa aataaacaag taggactaca 164040tcaaactaaa aagcttctgc atggcaaaag aaagtcaaca acatgaaaag gcaacctaca 164100gaatggaggg aaatatttgc aaaccatata cctgatgaga agttaatatc aaaatataga 164160aaatatataa ggaactcaca tatctcaata ccaaaaaaat aataacctgt tttataatgg 164220gcaaaggacc tgaatagaca ttttttcaaa gaagacacac agatggccaa cgagtgcagg 164280aaaaagcgtt caacatcact aatcatcagg gaaatgcaaa tcaaaaccac aatgagatat 164340cactccacac ctgttaggaa agctattatg aaaaacacaa gagacaatga atattggcaa 164400gggcatggag aaaaaagaac ccttgtacac tgttggtaga aatgtaaatt gcaacagcct 164460ttatggaaaa tagaatggag gttcctcaaa aaataaaaat agaactacca tacaatctag 164520caattccact tatgagtgta catccaaagg aatcaaatca ctatgtcaaa gagatatctg 164580tacttccttg tttattgcag ctttactcac agtagccaag ataaggaaaa aatctaaatg 164640tccatcaacg gataaagaaa atggggggag gggtgtgcat gtatacatac acaatggact 164700atttttcagc cataacaaag aaggaaaccc tgccatttgt gacgatatga atgaacccag 164760aggacattat gttaagtgaa ataagccaga cacagaaata caaatattgt atgatctcat 164820ttatatgcga atctaaaaat ttcaaacttg aagacgaata agtagaacag tatttatcag 164880gagctagggg atgtggggaa gaagcaaaat attgggcaaa gagtataaac tttcagttat 164940gagatgaagg ccaggtgcga tggctcatgt tggtaatccc aatactttgg aaggctgagg 165000cagagggatt gcttgagacc agcctaggca agaaagtgag acctcatctc tacaaaaaat 165060aaaaataaaa gaatcagctg ggagtggtgg catacacctg tagtcccagc tagtcaggag 165120gctgaggggg gaggatcatt tgaaattgga aagtcaaggc tgcagtcagc caagatagtg 165180ccactgcatt gcagcctggg tgacagagcg aaaccctgtc tcaaaaaaaa aaaaaaaaaa 165240aagatgaata agttctgggg atcaaatgta cagcatggtg actatagttt ataactgcgt 165300tattacttga aattggataa gagcagattt taagcatccc cagcaccccc ccaacataca 165360cacacacaaa tggtaactat aggtggtgat agatatgtta atttgactgt gacaatcagc 165420attcaatata tacatatatc aaatcatcac attttacccc ttgaaataga aactttgatt 165480tgtcaatcaa atattttaaa acgaaaataa tcataatatt aatatagcat acaggaaaaa 165540atttcgtatc tcgactgata cagaaaattt catgataaaa acactttaaa acaaaagaaa 165600taaaagggaa ctccctcaac ctgataaatg gcatctgtgg aaacccccag ctagcatcaa 165660acttaataca gaaaggctgg gtgttcacct cttgaaccag gaacaagaca aagatgcctg 165720cttttgccac ttccatttga ccttgtactg aggttctggc tagggcaatt atccctgaaa 165780aagaaataaa aggcttccaa ataaaagaag aagtaaaact atctctactc gctcatgaca 165840tgatcttgca tatagaaaat gtgcacatgt acacacacac aaaccattag aactaataaa 165900caagttcagc aagtttgcag aatataaaat gaatgtacaa aaatcaagtg tttttctata 165960tactagcaat taacaatctc aaaatgaatt aaaattccat ttacaatagt atcaaacata 166020aattatttag aaataaaaag tgcactgaaa actacaaaat attttgaaat aaatcagaaa 166080agatttaagt aaatggatca cttgaacctg ggaagcagag gttgcagtgt gccgagattg 166140taccactgca ctttagcctg ggcaacagag ggagactcca aagagtcgaa aagaaaagaa 166200aagatttaaa taagctgaaa catattctat ggatcagaag acttaatatt gttaaagtga 166260caatattccc caaattgatc tacagcttca actcaacccc tatcaaaatc ctagcttgct 166320ttttggctga aattgacaag ctgattctat aatttatatg gaatctcaaa ggatccagaa 166380taaccaaaac aatattgaaa aataaagaac agcgttggtg gattaacatt ttccaatttc 166440aaaacttact atagcactgc ggtaatcaag cagtgtggca ctgtatagca tgtacattac 166500agatcagtgg actagaatca atgtccagaa ataaaccgtt atgtttataa tgaattactt 166560tttaataagg tgtcaagaca acgcaatggg aaaagaataa tgaattcaac aaatgatgca 166620tggacaaccg gacatgcaca tgcaacacaa tgaatttgaa ttcttctatc gctccatgca 166680taaaaactaa ctcaaaatgg gtcacggatg taaatgaaaa gctaaaacta taataatcct 166740agaggaaaac ctaggagtaa atctttaaga tgttattgta ggcagtggtt tctcagatag 166800gaccccaaaa tcacaagcga caaaagaaat tggacttaaa gttaaatact tttgtgcttc 166860aaacatcatc aagaaagtga aaacacaacc cgcagaagca ataaaaatgt ctgtaagtca 166920tgtatccgat tagagacttc tatccaggat atataaataa tgcaattcaa tgataaaaaa 166980gataaatagc ccagttttcc aaagagtcaa gcatctgaat atacatctct ccaaaaatat 167040acagatatcc aacaagcatg tgaaaagatg ttcaaagcca tttgccaggt gcacaaaccc 167100aagacagtat gaggagatgc tacagggact ctgctgcttc acagacatga agcgttggtg 167160agaatgtagg cagccgcctt tggggacttc acatccccgc cgccccacgc acggtgagct 167220agtgtttaaa cttagccgag atcaatacac gcgactgtgt gcccgtcaga ccctgcgctg 167280ccggcggggc tgggagaggc gggcgccagg agtgggcggg aacctggggg tcaggcccca 167340gccgcgggaa gccgcccagg agcgcgcgaa accttctcca cacccttcca ggcatttgcc 167400cgccgcgatt cagagagccg acccgtgacc cctggcctcc cctagacagc cccgcatgtc 167460cagatgtgcc gtcccgcctg cctcccgcga ccactggcca tctctgggcc tgggcgcggt 167520ctcggcgccc gcctgccccc gccaggagcc gcaggtccag ccagtgaaga agcccgcgct 167580gaaggagcct ctgtgctcca gaatccatcc tcagtatcag cgctggggtg gcctcctcca 167640ggaagccctt ctgattctct catgggtcgc tcttcctctg cagactcccg gagcacccct 167700gctccaagta ccgcaagtgg cactgagaac ttggggagag cagaggctgt gcctagattt 167760gtagggagtc cccgcagctc caccccaggg cctacaggag cctggccttg ggcgaagccg 167820aggcaggcag gcagggcaaa gggtggaagc aattcaggag agaacgagtg aacgaatgga 167880tgaggggtgg cagccgaggt tgccccagtc ccctggctgc aggaacagac acctcgctga 167940ggagagaccc aggagcgagg cccctgcccc gcccgaggcg aggtcccgcc cagtcggcgc 168000cgcgtgaaga gtgggagaga atactgcggg ggcgggggcg ggggcggggg cgggggcggg 168060ggccgccggg agcctggagc cagaccgggc ggggccggca ccgggccagg gacagtgggg 168120gaggaggctg cgggctgagc gaccctgacc ccccccagtc cgcgctggtt ccgg 168174 64 21 DNA Homo sapiens 64aggagcgcgc raaaccttct c 21 65 18 DNA Homo sapiens 65tggacttaaa tacttttg 18 66 21 DNA Homo sapiens 66tcatgtatcc rattagagac t 21

Claims

1. A method for determining the identity of an allelic variant of a 5-LO gene in a nucleic acid obtained from a patient, wherein the sample comprises a 5-lipoxygenase (5-LO) gene sequence, comprising contacting a sample nucleic acid from the patient with a probe or primer having a sequence which is complementary to a 5-LO gene sequence, wherein the allelic variant comprises one or more nucleotide sequences selected from the group consisting of those set forth in SEQ ID NO: 4, SEQ ID NO:5, and SEQ ID NO:6, or the complements thereof, thereby determining the identity of the allelic variant.

2. The method of claim 1, wherein determining the identity of the allelic variant comprises determining the identity of at least one nucleotide at any one of the nucleotide residues selected from the group consisting of: residue 1000 of SEQ ID NO:1, any one of residues 472-477 of SEQ ID NO:1, and residue 559 of SEQ ID NO:1.

3. The method of claim 1, wherein determining the nucleotide content comprises sequencing the nucleotide sequence.

4. The method of claim 1, wherein determining the identity of the allelic variant comprises performing a restriction enzyme site analysis.

5. The method of claim 1, wherein determining the identity of the allelic variant is carried out by single-stranded conformation polymorphism.

6. The method of claim 1, wherein determining the identity of the allelic variant is carried out by allele specific hybridization.

7. The method of claim 1, wherein determining the identity of the allelic variant is carried out by primer specific extension.

8. The method of claim 1, wherein determining the identity of the allelic variant is carried out by an oligonucleotide ligation assay.

9. The method of claim 1, wherein the probe or primer comprises a nucleotide sequence from 15 to about 30 nucleotides.

10. The method of claim 1, wherein the probe or primer is labeled.