The present invention relates to a device for evaluating particles in a liquid and a method for operating the device.
A technique of fixing a part of a test liquid containing particles to a specific region while adjusting a gap between two transparent glass in accordance with a size of the particles in the liquid is known as means for automatically observing particles in a liquid such as microorganisms in a test liquid (Patent Literatures 1 and 2).
However, in the technique in the background art (Patent Literatures 1 and 2), drive members such as transparent glasses are moved up and down, and particles in the liquid to be observed are crushed and adhered to a drive member. It has been considered a problem that the adhered substances not only hinder automatic observation, but also requires a great burden to remove them.
Therefore, an object of the present invention is to provide a device for evaluating particles in a liquid, which can greatly prevent adhesion of particles in a liquid to a drive member or the like while observing the particles in a test liquid, with a suitable gap by driving the drive member in a opposite direction (up-down driving), and a method for operating the device.
In order to achieve the above object, one embodiment of the present invention includes a device for evaluating particles in a liquid of the present invention including:
a main body member including a first opening part and a second opening part facing each other, and an hollow part inside thereof;
a first flow channel and a second flow channel connected to the main body member and communicating with the hollow part;
a first drive member and a second drive member which are provided in the hollow part so as to face each other and being slidable within the hollow part;
a drive means for driving the first drive member and/or the second drive member in a facing direction within the hollow part;
a liquid feeding means for introducing a liquid containing particles into the hollow part through the first flow channel; and
an imaging means for capturing an image of particles within the hollow part through the first opening part or the second opening part,
in which when observing the first drive member and the second drive member from the facing direction, the first drive member and the second drive member include portions wider than the first flow channel and the second flow channel.
One embodiment of the present invention include a method for operating the device for evaluating particles in a liquid including driving the first drive member and/or the second drive member such that a width between the first drive member and the second drive member in the facing direction is 0.01 mm or more and less than 0.05 mm during observation, and is 0.5 mm or more at times other than the observation.
The device for evaluating particles in a liquid of the present invention, and the method for operating the device enable to observe the particles in a liquid contained in a test liquid in a suitable gap by driving a first drive member and a second drive member in a facing direction (up-down driving), and further enable to greatly prevent the particles in a liquid from adhesion to a drive member or the like. Thus, the accuracy of observation results can be improved and the burden of device maintenance can be greatly reduced.
Preferred embodiments of the present invention will be described below with reference to the drawings.
The device for evaluating particles in a liquid according to an embodiment of the present invention includes:
a main body member including a first opening part and a second opening part facing each other, and a hollow part inside of the body member;
a first flow channel and a second flow channel connected to the main body member and communicating with the hollow part;
a first drive member and a second drive member provided in the hollow part so as to face each other and being slidable within the hollow part;
a drive means for driving the first drive member and/or the second drive member in a facing direction within the hollow part;
a liquid feeding means for introducing a liquid containing particles into the hollow part through the first flow channel; and
an imaging means for capturing an image of particles within the hollow part through the first opening part or the second opening part,
in which when observing the first drive member and the second drive member from the facing direction, the first drive member and the second drive member include portions wider than the first flow channel and the second flow channel.
In the device for evaluating particles in a liquid according to the embodiment of the present invention, it is preferable that the first opening part and the second opening part face each other and communicate with the hollow part.
The device for evaluating particles in a liquid according to the embodiment of the present invention includes: a main body member including a first opening part and a second opening part facing each other and communicating with each other to form a hollow part, and a first flow channel and a second flow channel respectively communicating with the hollow part; a first drive member and a second drive member respectively fitted or loosely fitted to the hollow part; a drive means for driving the first drive member and/or the second drive member in a facing direction within the hollow part; a liquid feeding means for introducing a liquid containing particles into the hollow part through the first flow channel; and an imaging means for capturing an image of particles within the hollow part. When the first drive member and the second drive member are observed from the facing direction, the first drive member and the second drive member include portions wider than the first flow channel and the second flow channel.
The device for evaluating particles in a liquid according to the embodiment of the present invention may include a main body member including a first opening part and a second opening part facing each other and communicating with each other to form a hollow part, and a first flow channel and a second flow channel respectively communicating with the hollow part.
The first opening part 2 and the second opening part 3 have the same width in the main body member 1 of
The main body member includes a first opening part and a second opening part facing each other, and a hollow part inside of the body member.
The main body member may have a tubular shape, a substantially cylindrical shape, or a polygonal tubular shape. In addition, the first opening part may be provided at one end, and the second opening part may be provided at the other end.
The first opening part 2 and the second opening part 3 may communicate with each other to form a hollow part 6. In the device for evaluating particles in a liquid according to the embodiment of the present invention, a test liquid present in the hollow part 6 can be observed and evaluated through the first opening part 2 or the second opening part 3.
In the main body member 1 of
In the cross-sectional diagram of
In
The first flow channel 4 and the second flow channel 5 are connected to the main body member 1 separately and communicate with the hollow part 6.
Materials for forming the main body member are not particularly limited as long as they are not corroded by the test liquid, and examples thereof include metal such as stainless steel and aluminum.
The device for evaluating particles in a liquid according to the embodiment of the present invention requires a first drive member and a second drive member which are provided within the hollow part so as to face each other and which are slidable within the hollow part. In addition, the device for evaluating particles in a liquid of the present invention requires a drive means which drives the first drive member and/or the second drive member in a facing direction within the hollow part.
Here, “fitted” means that the first drive member and the second drive member are respectively fitted to the hollow part so that the hollow part is in a liquid-tight state.
The first drive member and/or the second drive member may be in a state where the hollow part is not liquid-tight, that is, may be “loosely fitted”.
Both of the first drive member and the second drive member may be driven within the hollow part, or only one of the first drive member and the second drive member may be driven within the hollow part.
In the latter case, the drive member on a non-driving side may be fixed by an adhesive or the like such that the first opening part or the second opening part, or a part of the hollow part is closed. Alternatively, a portion corresponding to the drive member on the non-driving side may be integrally molded together with the main body member. In these cases, with no strict interpretation, if it is assumed that the fixed drive member or the portion corresponding to the drive member does not exist, the first opening part and the second opening part can be regarded as parts which are in communication with each other to form a hollow part.
The first drive member and/or the second drive member may be driven not only within the hollow part but also from the inside of the hollow part to the outside of the hollow part.
In the device for evaluating particles in a liquid according to the embodiment of the present invention, a test liquid present in a partial region of the hollow part sandwiched by the first drive member and the second drive member is imaged and evaluated through the first drive member or the second drive member. Therefore, at least a part of the first drive member or the second drive member is preferably transparent or translucent. On the other hand, both of the drive members may include a transparent portion, or the entire drive member may be molded so as to be transparent.
Examples of a material for forming the transparent portion of the drive member include glass, an acrylic resin, and a polycarbonate resin. Examples of a material for forming a portion other than the transparent portion of the drive member include metal such as stainless steel and aluminum.
The device for evaluating particles in a liquid according to the embodiment of the present invention requires a liquid feeding means (not shown) which introduces a liquid containing particles into the hollow part through the first flow channel. In addition, the device for evaluating particles in a liquid according to the embodiment of the present invention requires an imaging means which captures an image of the particles in a liquid within the hollow part.
The liquid feeding means can introduce a liquid containing particles, that is, a test liquid, into the partial region of the hollow part sandwiched by the first drive member and the second drive member through the first flow channel. Then the imaging means can capture an image of the test liquid present in the partial region of the hollow part sandwiched by the first drive member and the second drive member and evaluate it. For example, the test liquid can be imaged and evaluated by an imaging means 9 through the first drive member 7 or the second drive member 8 in
Examples of the liquid feeding means include a turbo (non-positive displacement) type pump or a positive displacement pump.
Examples of the imaging means include a camera including an element typified by a CCD or a CMOS, and it is preferable to properly use element sensitivity such as color or monochrome or UV or IR depending on an evaluation object.
The test liquid introduced to the partial region of the hollow part through the first flow channel is discharged to the outside of the hollow part through the second flow channel. Although a test liquid may be continuously introduced into a partial region of the hollow part, a test liquid within the partial region of the hollow part is retained temporarily to be imaged and evaluated in this state, by stopping a liquid flow of a test liquid into the first flow channel and/or the second flow channel by providing a closing means in the first flow channel and/or the second flow channel, thereby.
In the device for evaluating particles in a liquid according to the embodiment of the present invention, when the first drive member and the second drive member are observed from a facing direction, the first drive member and the second drive member are required to include a portion that is wider than the first flow channel and the second flow channel.
In a case where the first drive member 7 or the second drive member 8 in
On the other hand, as shown in
When the lengths L3 of the first drive member 7 and the second drive member 8 is excessively short, it is more likely that the particles in a liquid is retained in the hollow part 6, or that cleaning of the hollow part 6 is insufficient. On the other hand, when L3 is excessively long, clogging of the particles in a liquid introduced into the hollow part 6 is more likely. Therefore, L3 is preferably similar in length to L2, and further, shapes of the first drive member 7 and the second drive member 8 when observing the first drive member 7 and the second drive member 8 in the facing directions of each other are more preferably substantially circular or polygonal, and are still more preferably substantially circular, from the viewpoint of preventing the retained particles in a liquid from being caught.
The width between the first drive member and the second drive member in the facing direction may be appropriately determined depending on an average size of the particles in a liquid or a depth of field of the imaging means provided in the device for evaluating particles in a liquid according to the embodiment of the present invention, and during the observation of the particles in a liquid, the width is preferably 0.01 mm or more when the particles in a liquid is activated sludge, from the viewpoint of imaging resolution and position repeatability of a drive member. In addition, the width between the first drive member and the second drive member in the facing direction during the observation of the particles in a liquid is preferably less than 0.05 mm from the viewpoint of preventing the particles in a liquid from being misrecognized due to overlapping in the up-down direction.
On the other hand, the width between the first drive member and the second drive member is preferably 0.5 mm or more in order to prevent clogging of the hollow part, at times other than the observation of the particles in a liquid. The width between the first drive member and the second drive member at times other than the observation of the particles in a liquid is preferably 1 mm or less in order to avoid an increase in the observation time due to the drive time. At times other than the observation of the particles in a liquid, the particles in a liquid adhered to the first drive member and the second drive member are likely to be released, thus, the introduction of a test liquid and the driving of the first drive member and the second drive member in the facing direction (up-down driving) are preferably repeated intermittently.
In the method for operating the device for evaluating particles in a liquid according to the embodiment of the present invention, the first drive member and/or the second drive member are driven such that the width between the first drive member and the second drive member in the facing direction is 0.01 mm or more and less than 0.05 mm during the observation, and is 0.5 mm or more at times other than the observation.
The first drive member and/or the second drive member are/is driven in the facing direction preferably at a speed of 5 mm/s or less in order to reduce the chance of destruction of the drive member or the like while making evaluation time including the imaging appropriate. In addition, from the viewpoint of work efficiency, it is preferable to set the speed to be 0.5 mm/s or more.
In the cross-sectional diagrams of
In the cross-sectional diagrams of
A shape of the coupling member may be appropriately determined depending on a material of the coupling member, a drive range of the first drive member, or the like. The coupling member may be an elastic member, and examples of the material of the coupling member include a resin, a rubber, or an elastomer that can be expanded and contracted while having a constant tear strength.
Examples of a method for attaching the coupling member 11 to each of the main body member and the first drive member include fixing by adhesion or the like.
In the cross-sectional diagram of
The present invention can be suitably used for evaluating a state of particles in a liquid, such as activated sludge in a water treatment tank.
Although the present invention has been described in detail using specific embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible without departing from the spirit and scope of the present invention.
This application is based on Japanese Patent Application No. 2018-16114 filed on Feb. 1, 2018, and the contents of which are incorporated herein by reference.
Number | Date | Country | Kind |
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2018-016114 | Feb 2018 | JP | national |
This is the U.S. National Phase application of PCT/JP2019/001557, filed Jan. 18, 2019, which claims priority to Japanese Patent Application No. 2018-016114, filed Feb. 1, 2018, the disclosures of each of these applications being incorporated herein by reference in their entireties for all purposes.
Filing Document | Filing Date | Country | Kind |
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PCT/JP2019/001557 | 1/18/2019 | WO | 00 |