Claims
- 1. A DNA molecule, the sequence of which encodes the amino acid sequence of SEQ ID NO: 18, wherein Xaa.sup.469 and Xaa.sup.470 are acidic amino acids and Xaa.sup.471 and Xaa.sup.472 are arbitrary amino acids.
- 2. The DNA molecule of claim 1, wherein the encoded polypeptide has Glu.sup.469, Asp.sup.470, Tyr.sup.471, and Glu.sup.472.
- 3. The DNA molecule of claim 1, wherein the encoded polypeptide has Asp.sup.469, Glu.sup.470, Ala.sup.471, and Asp.sup.472.
- 4. A DNA molecule having the nucleotide sequence of SEQ ID NO: 3.
- 5. A replicable recombinant vector comprising the DNA sequence of claim 1.
- 6. A vector comprising, in order (5'.fwdarw.3'):
- a promoter sequence;
- a ribosome binding sequence;
- an initiation codon;
- a sequence encoding a signal peptide;
- a sequence encoding a mature translation product according to claim 1;
- 7. A DNA molecule having the nucleotide sequence of SEQ ID NO: 4.
- a termination codon; and
- a polyA addition signal.
- 8. A cultured cell transformed with the vector of claim 5.
- 9. A process for making a modified thrombomodulin polypeptide comprising the steps of:
- cultivating the cell of claim 8 under conditions which permit the expression of the heterologous DNA, and
- collecting the polypeptides produced by said cell.
Priority Claims (2)
Number |
Date |
Country |
Kind |
2-335720 |
Nov 1990 |
JPX |
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3-30271 |
Feb 1991 |
JPX |
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CROSS REFERENCE TO RELATED APPLICATION
This is a division of application Ser. No. 08/014,723, filed on Feb. 8, 1993, now U.S. Pat. No. 5,273,962, which is a continuation-in-part of 07/796,336 filed Nov. 22, 1991, now abandoned.
Non-Patent Literature Citations (4)
Entry |
Jackman, R. W., et al. (1987) Proc. Natl. Acad. Sci. USA 84: 6425-6429. |
Parkinson, J. F., et al. (1990) Biochem. Biophys. Res. Comm. 169(1): 177-83. |
Nawa, K., et al. (1990) id. 171(2): 729-37. |
Bourdon, M. A., et al. (1986) J. Biol. Chem. 261(27): 12534-37. |
Divisions (1)
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Number |
Date |
Country |
Parent |
14723 |
Feb 1993 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
796336 |
Nov 1991 |
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