Claims
- 1. A recombinant DNA molecule comprising sequence comprising the following elements in the 5' to 3' direction, said elements which are operably linked:
- a translation initiation codon;
- four polylinker condons;
- a DNA sequence encoding the first part of a fusion protein, said DNa sequence encoding human plasminogen fibrin binding regions selected from the group consisting of (a) kringle 1, (b) kringle 4, (c) kringle 5, (d) kringle 4 and kringle 5, and (e) kringle 1 and kringle 2 and kringle 3 and kringle 4;
- six polylinker condons; and
- a DNA sequence encoding the second part of a fusion protein, said DNA sequence encoding streptokinase derived from Streptococcus equisimilis.
- 2. The recombinant DNA molecule of claim 1 wherein the DNA sequence encoding human plasminogen fibrin binding regions is derived from one of the following plasmids: pKSK056, pKSK036, pKSK029, pKSK351, and pKSK067.
- 3. The recombinant DNA molecule of claim 1 wherein the DNA sequence encoding streptokinase is derived from Streptoccus equisimilis H46A.
- 4. A recombinant DNA molecule comprising a sequence comprising the following elements in the 5' to 3' direction, said elements which are operably linked:
- a DNA sequence encoding the first part of a fusion protein, said DNA sequence encoding human plasminogen fibrin binding regions selected from the group consisting of (a) kringle 1, (b) kringle 4, (c) kringle 5, (d) kringle 4 and kringle 5, and (e) kringle 1 and kringle 2 and kringle 3 and kringle 4; and
- a DNA sequence encoding the second part of a fusion protein, said DNA sequence encoding streptokinase derived from Streptococcus equisimilis.
- 5. The recombinant DNA molecule of claim 4 wherein the dNA sequence encoding human plasminogen fibrin binding regions is derived from one of the following plasmids: pKSK056, pKSK036, pKSK029, pKSK351, and pKSK067.
- 6. The recombinant DNA molecule of claim 4 wherein the DNA sequence encoding streptokinase is derived from Streptococcus equisimilis H46A.
CROSS REFERENCE TO RELATED APPLICATIONS
This is a continuation of co-pending application U.S. Ser. No. 07/348,206 filed on May 9, 1989, entitled "HYBRID STREPTOKINASES WITH FIBRIN BINDING DOMAINS AND METHODS FOR THE SYNTHESIS OF SAME," now abandoned which is a continuation-in-part of U.S. Ser. No. 07/212,254 filed on Jun. 27, 1988, entitled "STREPTOKINASE CODING RECOMBINANT VECTORS," now U.S. Pat. No. 5,066,589, which is a continuation of U.S. Ser. No. 06/585,417 filed on Mar. 2, 1984, entitled "STREPTOKINASE CODING RECOMBINANT VECTORS," now U.S. Pat. No. 4,764,469.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4764469 |
Ferretti et al. |
Aug 1988 |
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Foreign Referenced Citations (1)
Number |
Date |
Country |
293934 |
Dec 1988 |
EPX |
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Continuations (2)
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Date |
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Parent |
348206 |
May 1989 |
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Parent |
585417 |
Mar 1984 |
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Continuation in Parts (1)
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Parent |
212254 |
Jun 1988 |
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