DNA molecules encoding murine son of sevenless (mSOS) gene and mSOS polypeptides

This invention broadly relates to polynucleotides encoding mammalian Sos gene and protein product thereof.
Protein tyrosine kinases (PTKs) are important regulatory proteins that control many aspects of cellular growth, differentiation, and metabolism (Cantley et al., 1991). Many polypeptide hormones are known to regulate the metabolism, differentiation, and growth of target cells by binding transmembrane receptors that possess intracellular PTK domains (Cross and Dexter, 1991).
The biological effects of PTK activation throughout mammalian cells may be mediated, at least in part, via the Ras proteins (Simon et al., Cell, Vol. 67, 701-716). Ras genes and protein products are associated with a variety of human tumours, particularly where Ras is overproduced or inappropriately expressed.
Simon et al. (Supra) identified a Drosophila gene, associated with protein tyrosine kinase signalling. The Drosophila son of sevenless (Sos) gene product is homologous to a yeast protein (CDC25), which is an activator of guanine nucleotide exchange by Ras proteins. The data of Simon et al. (Supra) indicates that tyrosine kinase signalling is effected by activation of the Sos protein (such as via tyrosine phosphorylation, or some form of indirect stimulation), which then activates Ras proteins by exchange GDP for GTP.
We have surprisingly identified the mammalian Sos gene, hereinafter referred to as mSos, fragments thereof, which have homology to various guanine exchange factors.
In accordance with a first aspect of this invention, there is provided a polynucleotide encoding an mSos gene, or a fragment or analogue thereof. This invention includes any mammalian Sos including human, domestic animal (sheep, cattle, etc) and companion animal (cats, dog, etc) mSos.
The term polynucleotide as referred to herein refers to DNA and RNA, and derivatives thereof as are well known in the art
Partial nucleotide sequences of the murine mSos genes mSos1 (SEQ ID NO:1) and mSos2 (SEQ ID NO:3) are shown in FIGS. 1 and 2. This invention includes such polynucleotide sequences and their full length equivalents, as well as analogues thereof where one or more nucleotides are substituted, deleted or inserted. Methods for the generation of polynucleotide analogues are well known in the art.
This invention includes vectors, such as plasmid, viral or other vectors as are well known in the art, which include a polynucleotide encoding mSos or a fragment or analogue thereof.
In another aspect of this invention, there is provided an mSos polypeptide or fragment or analogue thereof. The amino acid sequence of two murine mSos polypeptides, referred to as mSos 1 (SEQ ID NO:2) and mSos 2 (SEQ ID NO:4) are shown in FIGS. 1 and 2, compared to the drosophila Sos protein product. This invention extends to any mammalian mSos polypeptide, fragment or analogue thereof, including human, domestic animal and companion animal mSos polypeptide.
Reference to mSos polynucleotide fragments, or mSos polypeptide fragments, is to be taken to mean fragments which are unique to mSos. Such fragments will generally comprise in excess of twenty nucleotides, or 20 amino acids, and in particular correspond to a central domain of about 430 amino acids, which is homologous to a number of yeast guanine nucleotide exchange factors (as depicted in FIGS. 3a and 3b (SEQ ID NOS:1 and 3)).
Analogues of mSos polypeptides include amino acid modifications, deletions, substitutions, derivitizations and insertions of one or more amino acids.
It is stressed that this invention extends to mammalian Sos (mSos), in particular human. While this invention is exemplified with reference to murine Sos, the invention is clearly not so limited. Reference to mSos means any mammalian Sos gene or polypeptide, which may be isolated and characterised in accordance with this invention. For example, human mSos polynucleotides may be isolated by hybridization of murine mSos polynucleotides or fragments thereof to human cDNA or genomic sequences followed by isolation and characterisation of hybridising species. Mammals may have several forms of mSos, all of which are involved as regulators/effectors of tyrosine kinase signalling. All such forms of mSos are within the scope of this invention.
Mutations in the natural mammalian Sos genes, and gene products, may result in specific genetic defects, or tumour formation, given the role of mSos in PTK activation via Ras genes and protein products.
Accordingly, this invention extends to a method of detecting mutant mSos genes in an individual associated with a pathological phenotype (where pathological phenotype refers to any pathological condition), which method comprises comparing the nucleotide sequence or chromosomal location or structure of a suspected mutant mSos gene with a reference non mutated mSos gene as herein described. Suitable comparisons may be made by nucleotide sequencing, restriction fragment polymorphism, analysis of amplified products (such as by PCR), and other well known techniques in the art. Such methods may be used in genetic counselling, prenatal diagnosis or the like.
According to a further aspect of this invention there is provided an antagonist of mSos which comprises a compound having the same or similar three-dimensional structure as an mSos polypeptide or a fragment thereof, which blocks the interations between mSos and its substrate in the PTK signalling pathway. The invention further relates to a polynucleotide capable of blocking transcription or translation of mSos or fragments thereof. The polynucleotide may be in the form of a triple helix forming polynucleotide, an antisense polynucleotide or a ribozyme, as are well known in the art.

This invention will now be described by way of example only, with reference to the following non-limiting Figures and Examples. In the Figures:
FIG. 1: shows the nucleotide sequence (SEQ ID NO:1) and predicted amino acid sequence (SEQ ID NO:2) of the mSos 1 gene;
FIG. 2: shows the nucleotide sequence (SEQ ID NO:3) and predicted amino acid sequence (SEQ ID NO:4) of the mSos 2 gene;
FIG. 3: shows the partial nucleotide sequence of mSos 1 and mSos 2 wherein the nucleotide sequences are aligned to maximise homology;
FIG. 4a: Predicted amino acid sequence of the mSos1 (SEQ ID NO:6) and mSos2 (SEQ ID NO:4) genes and their aligned with Drosophila Sos4(SEQ ID NO:5). Identical residues are in black boxes, conservative substitutions in grey boxes.
FIG. 4b: Alternate aminoterminal coding regions of the mSos1 gene (SEQ ID NOS:7 and 8) identified by 5' RACE (Frohman, M. A. and Martin, G. R., 1988). The sequence of the mSos1 gene was derived from a composite of cDNA clones and 5' RACE products. The majority of RACE products (type 2) (SEQ ID NO:8) terminated before reaching a potential initiating methionine in a highly GC region that could not be processed by reverse transcriptase. An alternate RACE product was identified (type 1) (SEQ ID NO:7) which diverged from this sequence as indicated and extended to a potential methionine initiation codon and upstream stop codon. The molecular weight of the predicted protein is 150 kd;
FIG. 5a: Alignment of the predicted Drosophila (SEQ ID NO:9) and mouse Sos proteins (SEQ ID NOS:10 and 11) with four related yeast proteins cdc25 (SEQ ID NO:12), sdc25 (SEQ ID NO:13), ste6 (SEQ ID NO:14) and buds (SEQ ID NO:15).
FIG. 5b: Schematic representation showing the size of each protein and the relative position of the conserved domain shown in (a) within each protein. Percentages indicate the degree of amino acid identity between a given domain and cdc25; and
FIG. 6: Northern blot analysis of RNA derived from a range of (a) mouse embryonic and adult tissues and (b) continuous mouse and human cell lines. Transcripts of 5.4 kb and 8.4 kb were detected with an mSos1 probe in all tissues and cell lines tested. Variation in signal intensity was mainly due to differences in the amount and quality of RNA (see control CAODH probe). Additional smaller transcripts were apparent in testes RNA. A single major transcript of 5.6 kb was detected with an mSos2 probe, expressed at apparently comparable levels of mSos1. Adult tissues are indicated. Embryonic tissues were from heads and bodies of embryos whose stage of gestation was estimated from the time of plugging of the donor females. Mouse cell lines are, 3T3, Balb/c fibroblast; FN4, erythroid; FDCP1, myeloid; J774, macrophage; ABLS8, preB lymphoid, W279, B lymphoid, MPC11, plasmacytoma; EL4, early T lymphoid and W7.1, T lymphoid. Transcripts of the same size were also detected in RNA from the human erythroleukaemic cell line K562. GAPDH was used as a control probe for the amount and quality of RNA.

EXAMPLE 1
A fragment of the Drosophila Sos gene (corresponding to amino acids 841-1303, Simon et al., Supra) was used to screen mouse embryonic eye and adult brain cDNA libraries at low stringency.
cDNA clones were isolated from random bred Swiss E17 embryonic eye and Balb/c adult brain (Stratagene) cDNA libraries using a random Sos cDNA fragment. Duplicate nitrocellulose filters were hybridized in 5.times.SSC, 5.times.Denharts, 5 mM EDTA, 100 .mu.g/ml herring testes DNA, 0.1% SDS at 65.degree. C. for 18 hr and washed in 2.times.SSC, 0.1% SDS at 50.degree. C. Partial DNA sequence obtained from two clones confirmed the isolation of two Sos related genes termed mSos1 and mSos2. Subsequent screening of the eye and brain libraries with these cDNA inserts identified the remainder of the clones, present at approximately 1;150,000 (eye) and 1;250,000 (brain) recombinants. S5' RACE of mSos1 was performed using RNA derived from an adult mouse brain as described previously (Frohman and Martin, 1988) and also with the modifications of using thermostable reverse transcriptase Tet-Z, Amersham) and prior denaturation of RNA with methyl mercury (Sambrook et al, 1989). Double stranded dideoxy chain termination DNA sequencing was performed on either nested deletions of cDNA cloned in Bluescript KS or with specific oligonucleotide primers using standard methods (Sambrook et al., Supra). The sequence of both strands was obtained and nucleotide sequence of mSos 1 and 2 is shown in FIGS. 1 and 2, (SEQ ID NOS:1 and 3, respectively).
FIG. 3 shows alignment of sequences of partial sequences of mSos 1 and mSos 2 to maximise homology. The nucleotide numbering of FIG. 3 differs from FIGS. 1 and 2, as in FIGS. 1 and 2 the protein sequence starts at position 18, that is, the second methionine residue.
FIG. 4 shows the amino acid sequence of Drosophila (Sos) and mouse Sos (mSos) proteins. The sequence is presented using the standard one letter amino acid code The molecular weight of the predicted protein is about 150 kd The correct amino acid sequence numbering is shown in FIGS. 1 and 2 with the second methinone residue in mSos representing the start of translation.
The predicted amino acid sequence for mSos2 (SEQ ID NO:4) extended to within 85 amino acids of the aminoterminal end of Sos (FIG. 4a). Sos shows an overall amino acid identity to both mSos1 and mSos2 of 45%. Both mSos1 and mSos2 remain colinear with SoS over their coding regions, with the exception of their carboxyterminal ends where homology between Sos and the murine genes is more scattered. Comparison of the two murine genes shows that they share approximately 67 amino acid identity, with the lowest degree of similarity residing in the final 270 amino acids (41% identity, FIG. 4a). No significant areas of homology were identified when the untranslated regions of the two genes were compared.
FIG. 5 shows alignment of the predicted Drosophila and mouse Sos proteins with four related yeast proteins cdc25, sdc25, ste6 and bud5.
The most notable feature of the Sos and mSos genes is a central domain of approximately 430 amino acids which shows a high degree of homology to several yeast guanine nucleotide exchange factors, including cdc25, sdc25 and ste6. This domain is present in a fragment of the sdc25 gene which is capable of catalysing nucleotide exchange by either the yeast RAS2 protein or human c-ras (Crechet at al., 1990), suggesting that the region of homology between the yeast, Drosophila and mouse genes defines a domain that catalyses nucleotide exchange on ras proteins. Inclusion of the mSos1 and mSos2 in this alignment further highlights residues which are highly conserved between the members of this gene family (FIG. 3a). Similarity between Sos and Sos and the yeast genes is limited to this domain.
We have also identified a second domain on both the mSos 1 (amino adds 200 to 500 of FIG. 1) and mSos 2 predicted polypeptides which has homology with the dbl protein, which is a guanine exchange factor for a ras-related protein. This suggests that mSos proteins may have separate guanine exchange domains of ras and for ras related proteins.
EXAMPLE 2
RNA isolated from various murine development stages, adult tissues, haemopoietic cell lines, and a human cell line was analysed for Sos transcripts.
Polyadenylated RNA was isolated from tissues and cell lines by disruption in proteinase K and SDS (Gonda, 1985) and oligo-(dT) affinity chromatography. Two micrograms of RNA from each source was subjected to formaldehyde agarose gel electrophoresis (Sambrook et al, Supra), transferred overnight to Hybond C-super (Amersham) in 20.times.SSC and then baked for 2 hrs. Filters were prehybridized from 4-6 h in 50% formamide, 5.times.SSC, 5.times.Denhardts, 5 mM EDTA, 100 .mu.g ml-1 herring tests DNA and 0.5% SDS at 42.degree. C. and then hybridized for 18 h. The 32P labelled probes were from nt299 to 4464 of mSos1 and from nt1 to 3801 of the available mSos2 sequence. Identical results were obtained with non-overlapping probes from the 3' untranslated region of each gene (data not shown). Washes were performed in 0.2.times.SSC, 0.3% SDS at 65.degree. C. and the filter autoradiographed for 3 days at -70.degree. C. in the presence of an intensifying screen.
As shown in FIG. 6, the mSos1 gene encodes two distinct transcripts of 5.4 kb and 8.4 kb that are present in approximately equal abundance. Additional smaller transcripts of 4.8 kb and 3.9 kb were detected in RNA derived from testes (FIG. 6a). The mSos2 gene appears to encode a single transcript of 5.6 kb. As a cDNA of 5.3 kb has been obtained for mSos2 this suggests that the majority of the coding region has been obtained for this gene. To investigate whether there was any lineage restriction in expression of either gene within a given tissue, we tested RNA expression in haemopoietic cell lines that were representative of early and late lymphoid, myeloid and erythroid lineages. Expression of both mSos1 and mSos2 was detected in all haemopoietic lineages tested and was comparable to that in Balb/c 3T3 fibroblats (FIG. 6b). The broad pattern of expression obtained with mSos1 and mSos2 is consistent with a postulated role in regulating the widely expressed ras proteins.
Northern and Southern blot analysis was also performed using human RNA and DNA. Transcripts corresponding in size to those from mSos1 and mSos2 were present in RNA from the human erythroleukaemic cell line K562 (Lozzio and Lozzio, 1975) (FIG. 6b) and mSos1 and mSos2 hybridized with human genomic DNA when probed at high stringency (data not shown), indicating the presence of separate human genes closely related to both mSos1 and mSos2, which may be regarded as the human mSos genes.
This invention has been described by way of Example only, and includes all modifications, variations, nucleotide sequences and fragments/analogues thereof, and protein sequences and fragments and analogues thereof as herein described. Human Sos genes and polypeptides, as well as fragments and analogues thereof, may be isolated and characterised according to these Examples.
REFERENCES
Cantley, L. C., et al. (1991) Cell 64, 281-302.
Crechet, J., et al. (1990) Science 248, 866-868.
Cross, M., and Dexter, T. M. (1991) Cell 64, 271-280.
Frohman, M. A., and Martin, G. R (1988) Proc. Natl. Acad. Sci. 85, 8998-9002.
Gonda, T. J., et al. (1985) Embo J. 4, 2003-2008.
Lozzio, C. B., and Lozzio, B. B. (1975) Blood 45, 321-334.
Sambrook, J. et al. (1989) Molecular Cloning, Cold Spring Harbor Laboratory, New York.
__________________________________________________________________________SEQUENCE LISTING(1) GENERAL INFORMATION:(iii) NUMBER OF SEQUENCES: 15(2) INFORMATION FOR SEQ ID NO:1:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 4716 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(ix) FEATURE:(A) NAME/KEY: CDS(B) LOCATION: 22..3975(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:AAGCAGCACCCCGCGGGCACCATGCAGGCGCAGCAGCTGCCTTACGAGTTT51MetGlnAlaGlnGlnLeuProTyrGluPhe1510TTCAGCGAGGAGAACGCGCCCAAGTGGCGGGGGCTGCTGGTGCCTGCG99PheSerGluGluAsnAlaProLysTrpArgGlyLeuLeuValProAla152025CTGAAAAAGGTTCAGGGGCAAGTTCACCCTACTCTTGAGTCTAATGAT147LeuLysLysValGlnGlyGlnValHisProThrLeuGluSerAsnAsp303540GATGCTCTTCAGTATGTTGAAGAATTAATTTTGCAATTACTAAATATG195AspAlaLeuGlnTyrValGluGluLeuIleLeuGlnLeuLeuAsnMet455055CTATGCCAAGCTCAGCCCCGGAGTGCTTCAGATGTGGAGGAACGTGTT243LeuCysGlnAlaGlnProArgSerAlaSerAspValGluGluArgVal606570CAAAAGAGTTTTCCTCATCCAATTGATAAGTGGGCAATAGCTGATGCC291GlnLysSerPheProHisProIleAspLysTrpAlaIleAlaAspAla75808590CAATCAGCCATTGAAAAGAGGAAGAGACGAAATCCTTTATCGCTGCCA339GlnSerAlaIleGluLysArgLysArgArgAsnProLeuSerLeuPro95100105GCAGAAAGAATTCATCATTTATTAAGGGAGGTCCTCGGTTATAAAATT387AlaGluArgIleHisHisLeuLeuArgGluValLeuGlyTyrLysIle110115120GACCACCAGGTTTCTGTTTACATAGTAGCAGTATTAGAATACATTTCT435AspHisGlnValSerValTyrIleValAlaValLeuGluTyrIleSer125130135GCAGATATTTTAAAGCTCGTGGGGAATTATGTAAGAAATATACGGCAT483AlaAspIleLeuLysLeuValGlyAsnTyrValArgAsnIleArgHis140145150TATGAAATTACAAAACAAGACATTAAAGTGGCAATGTGTGCTGATAAG531TyrGluIleThrLysGlnAspIleLysValAlaMetCysAlaAspLys155160165170GTATTGATGGATATGTTTCATCAAGATGTAGAAGATATAAATATCTTA579ValLeuMetAspMetPheHisGlnAspValGluAspIleAsnIleLeu175180185TCTTTAACTGATGAAGAGCCTTCCACCTCAGGAGAACAAACTTATTAT627SerLeuThrAspGluGluProSerThrSerGlyGluGlnThrTyrTyr190195200GATTTGGTAAAAGCATTCATGGCAGAAATTCGACAGTATATAAGAGAA675AspLeuValLysAlaPheMetAlaGluIleArgGlnTyrIleArgGlu205210215TTAAATCTAATTATAAAAGTTTTTCGAGAGCCCTTTGTCTCTAATTCC723LeuAsnLeuIleIleLysValPheArgGluProPheValSerAsnSer220225230AAATTGTTTTCATCTAATGATGTAGAAAACATATTCAGTCGTATAGTA771LysLeuPheSerSerAsnAspValGluAsnIlePheSerArgIleVal235240245250GATATACATGAACTTAGTGTAAAGTTACTGGGCCATATAGAAGATACT819AspIleHisGluLeuSerValLysLeuLeuGlyHisIleGluAspThr255260265GTAGAAATGACAGATGAAGGCAGTCCCCACCCATTAGTAGGAAGCTGT867ValGluMetThrAspGluGlySerProHisProLeuValGlySerCys270275280TTTGAAGACTTAGCAGAAGAACTGGCATTTGACCCGTATGAGTCATAT915PheGluAspLeuAlaGluGluLeuAlaPheAspProTyrGluSerTyr285290295GCTCGGGATATTTTACGACCCGGATTCCATGGCCATTTTCTTAGTCAG963AlaArgAspIleLeuArgProGlyPheHisGlyHisPheLeuSerGln300305310TTATCAAAGCCTGGGGCAGCACTTTATTTGCAGTCCATAGGCGAAGGC1011LeuSerLysProGlyAlaAlaLeuTyrLeuGlnSerIleGlyGluGly315320325330TTCAAAGAAGCTGTCCAGTACGTCCTGCCCCGGCTGCTGCTTGCCCCT1059PheLysGluAlaValGlnTyrValLeuProArgLeuLeuLeuAlaPro335340345GTGTACCACTGTCTGCATTACTTTGAACTTCTGAAGCAGTTAGAAGAA1107ValTyrHisCysLeuHisTyrPheGluLeuLeuLysGlnLeuGluGlu350355360AAGAGTGAAGATCAAGAAGACAAGGAGTGTATGAAGCAAGCAATAACA1155LysSerGluAspGlnGluAspLysGluCysMetLysGlnAlaIleThr365370375GCCCTGCTTAATGTCCAAAGTGGCATGGAAAAAATTTGCTCCAAAAGT1203AlaLeuLeuAsnValGlnSerGlyMetGluLysIleCysSerLysSer380385390CTTGCAAAACGAAGACTAAGTGAGTCTGCATGTCGGTTTTACAGCCAG1251LeuAlaLysArgArgLeuSerGluSerAlaCysArgPheTyrSerGln395400405410CAGATGAAGGGGAAACAGCTAGCCATCAAGAAGATGAACGAGATCCAG1299GlnMetLysGlyLysGlnLeuAlaIleLysLysMetAsnGluIleGln415420425AAGAACATTGATGGCTGGGAGGGGAAGGACATTGGACAGTGTTGCAAT1347LysAsnIleAspGlyTrpGluGlyLysAspIleGlyGlnCysCysAsn430435440GAGTTCATAATGGAAGGAACTCTTACACGTGTAGGAGCCAAACACGAG1395GluPheIleMetGluGlyThrLeuThrArgValGlyAlaLysHisGlu445450455AGACACATATTTCTCTTCGATGGCTTAATGATTTGCTGTAAATCAAAC1443ArgHisIlePheLeuPheAspGlyLeuMetIleCysCysLysSerAsn460465470CATGGGCAGCCAAGACTCCCTGGTGCTAGCAGTGCAGAATACCGGCTT1491HisGlyGlnProArgLeuProGlyAlaSerSerAlaGluTyrArgLeu475480485490AAAGAAAAGTTTTTTATGCGAAAGGTACAGATTAATGATAAAGATGAC1539LysGluLysPhePheMetArgLysValGlnIleAsnAspLysAspAsp495500505ACCAGTGAGTACAAGCATGCTTTTGAAATCATTCTGAAAGATGGCAAT1587ThrSerGluTyrLysHisAlaPheGluIleIleLeuLysAspGlyAsn510515520AGTGTTATATTTTCTGCCAAGTCAGCTGAAGAGAAAAACAACTGGATG1635SerValIlePheSerAlaLysSerAlaGluGluLysAsnAsnTrpMet525530535GCAGCACTGATCTCTTTGCAGTACCGCAGCACCCTGGAGAGGATGCTG1683AlaAlaLeuIleSerLeuGlnTyrArgSerThrLeuGluArgMetLeu540545550GACGTAACGGTGCTGCAGGAGGAGAAGGAGGAGCAGATGAGGCTGCCC1731AspValThrValLeuGlnGluGluLysGluGluGlnMetArgLeuPro555560565570AGTGCTGAAGTGTACAGGTTTGCAGAACCTGACTCCGAGGAGAATATT1779SerAlaGluValTyrArgPheAlaGluProAspSerGluGluAsnIle575580585CTATTCGAAGAGAATGTGCAGCCCAAAGCTGGGATCCCCATTATCAAG1827LeuPheGluGluAsnValGlnProLysAlaGlyIleProIleIleLys590595600GCAGGGACAGTGCTTAAGCTCATTGAGAGGCTTACCTACCACATGTAC1875AlaGlyThrValLeuLysLeuIleGluArgLeuThrTyrHisMetTyr605610615GCAGATCCAAATTTTGTTCGGACGTTTCTTACAACATACAGGTCCTTT1923AlaAspProAsnPheValArgThrPheLeuThrThrTyrArgSerPhe620625630TGCAGACCTCAAGAACTACTGAGTCTTCTGATAGAAAGATTTGAAATT1971CysArgProGlnGluLeuLeuSerLeuLeuIleGluArgPheGluIle635640645650CCAGAGCCTGAGCCAACAGAAGCTGATCGCATAGCTATAGAGAATGGA2019ProGluProGluProThrGluAlaAspArgIleAlaIleGluAsnGly655660665GATCAGCCCCTGAGTGCAGAGCTGAAGAGGTTTAGAAAGGAATATATT2067AspGlnProLeuSerAlaGluLeuLysArgPheArgLysGluTyrIle670675680CAGCCTGTGCAGTTGAGGGTGTTAAATGTGTGTCGGCACTGGGTGGAG2115GlnProValGlnLeuArgValLeuAsnValCysArgHisTrpValGlu685690695CACCATTTCTATGACTTTGAAAGAGATGCAGACCTTTTACAGAGAATG2163HisHisPheTyrAspPheGluArgAspAlaAspLeuLeuGlnArgMet700705710GAGGAATTTATTGGAACAGTAAGAGGTAAAGCAATGAAAAAATGGGTC2211GluGluPheIleGlyThrValArgGlyLysAlaMetLysLysTrpVal715720725730GAATCCATCACTAAGATAATCCAAAGGAAAAAAATTGCAAGAGACAAT2259GluSerIleThrLysIleIleGlnArgLysLysIleAlaArgAspAsn735740745GGCCCAGGTCATAACATTACATTTCAGAGCTCACCTCCCACAGTTGAG2307GlyProGlyHisAsnIleThrPheGlnSerSerProProThrValGlu750755760TGGCACATAAGCAGACCTGGGCACATAGAGACTTTTGACTTGCTCACC2355TrpHisIleSerArgProGlyHisIleGluThrPheAspLeuLeuThr765770775TTACACCCAATAGAAATTGCTCGGCAACTCACTTTACTTGAATCAGAT2403LeuHisProIleGluIleAlaArgGlnLeuThrLeuLeuGluSerAsp780785790CTATACCGGGCTGTGCAGCCATCAGAATTAGTTGGAAGTGTGTGGACA2451LeuTyrArgAlaValGlnProSerGluLeuValGlySerValTrpThr795800805810AAAGAAGATAAAGAAATTAATTCTCCCAACCTTCTGAAGATGATTCGG2499LysGluAspLysGluIleAsnSerProAsnLeuLeuLysMetIleArg815820825CACACCACTAACCTCACTTTGTGGTTTGAGAAATGTATTGTAGAAACA2547HisThrThrAsnLeuThrLeuTrpPheGluLysCysIleValGluThr830835840GAAAACTTAGAAGAAAGAGTAGCTGTAGTAAGTCGGATAATTGAGATT2595GluAsnLeuGluGluArgValAlaValValSerArgIleIleGluIle845850855CTACAAGTCTTTCAAGAGCTGAACAACTTCAATGGTGTCCTGGAAGTT2643LeuGlnValPheGlnGluLeuAsnAsnPheAsnGlyValLeuGluVal860865870GTCAGTGCTATGAACTCGTCACCTGTTTACAGACTAGACCACACATTT2691ValSerAlaMetAsnSerSerProValTyrArgLeuAspHisThrPhe875880885890GAGCAAATACCAAGCAGACAAAAGAAAATTTTAGAAGAAGCTCATGAA2739GluGlnIleProSerArgGlnLysLysIleLeuGluGluAlaHisGlu895900905TTGAGTGAAGATCACTATAAGAAATATTTGGCAAAACTCAGGTCTATT2787LeuSerGluAspHisTyrLysLysTyrLeuAlaLysLeuArgSerIle910915920AATCCACCGTGTGTGCCTTTCTTTGGAATTTATCTCACAAATATCCTG2835AsnProProCysValProPhePheGlyIleTyrLeuThrAsnIleLeu925930935AAGACAGAAGAGGGCAACCCTGAGGTCCTGAGGAGACACGGGAAAGAG2883LysThrGluGluGlyAsnProGluValLeuArgArgHisGlyLysGlu940945950CTTATTAACTTCAGCAAGAGGAGGAGAGTGGCCGAGATCACAGGCGAG2931LeuIleAsnPheSerLysArgArgArgValAlaGluIleThrGlyGlu955960965970ATCCAGCAGTACCAGAACCAGCCCTACTGCTTACGGGTGGAGCCGGAC2979IleGlnGlnTyrGlnAsnGlnProTyrCysLeuArgValGluProAsp975980985ATCAAGAGGTTCTTTGAAAACTTGAATCCAATGGGAAACAGCATGGAG3027IleLysArgPhePheGluAsnLeuAsnProMetGlyAsnSerMetGlu9909951000AAAGAATTTACAGACTATCTGTTCAACAAATCCCTAGAAATAGAACCC3075LysGluPheThrAspTyrLeuPheAsnLysSerLeuGluIleGluPro100510101015CGGCACCCTAAGCCTCTTCCGAGATTCCCAAAAAAATACAGCTATCCC3123ArgHisProLysProLeuProArgPheProLysLysTyrSerTyrPro102010251030CTAAAATCTCCTGGTGTTCGTCCATCAAATCCAAGACCAGGAACCATG3171LeuLysSerProGlyValArgProSerAsnProArgProGlyThrMet1035104010451050AGACATCCCACACCTCTGCAGCAGGAGCCAAGAAAAATTAGCTACAGT3219ArgHisProThrProLeuGlnGlnGluProArgLysIleSerTyrSer105510601065CGGATTCCTGAAAGTGAGACGGAAAGCACAGCATCTGCACCAAACTCC3267ArgIleProGluSerGluThrGluSerThrAlaSerAlaProAsnSer107010751080CCTCGGACCCCACTGACGCCGCCCCCTGCATCTGGCACCTCCAGCAAC3315ProArgThrProLeuThrProProProAlaSerGlyThrSerSerAsn108510901095ACAGATGTTTGCAGCGTGTTCGATTCTGACCACTCGGCAAGCCCTTTT3363ThrAspValCysSerValPheAspSerAspHisSerAlaSerProPhe110011051110CATTCAAGATCTGCTTCAGTCTCATCTATAAGTTTATCCAAGGGCACT3411HisSerArgSerAlaSerValSerSerIleSerLeuSerLysGlyThr1115112011251130GATGAAGTGCCTGTCCCCCCTCCTGTACCCCCTCGAAGACGTCCAGAG3459AspGluValProValProProProValProProArgArgArgProGlu113511401145TCTGCCCCAGCTGAATCCTCCCCATCCAAGATTATGTCTAAGCACTTG3507SerAlaProAlaGluSerSerProSerLysIleMetSerLysHisLeu115011551160GACAGCCCCCCAGCTATTCCTCCTAGGCAACCCACATCCAAAGCCTAT3555AspSerProProAlaIleProProArgGlnProThrSerLysAlaTyr116511701175TCACCACGCTATTCAATATCAGATCGGACCTCTATATCAGATCCTCCT3603SerProArgTyrSerIleSerAspArgThrSerIleSerAspProPro118011851190GAAAGCCCTCCCTTGTTACCACCACGGGAACCTGTGAGGACACCTGAT3651GluSerProProLeuLeuProProArgGluProValArgThrProAsp1195120012051210GTTTTCTCAAGCTCACCATTACATCTCCAACCTCCTCCTTTGGGCAAA3699ValPheSerSerSerProLeuHisLeuGlnProProProLeuGlyLys121512201225AAGAGTGATCATGGCAACGCCTTCTTCCCAAACAGCCCATCCCCTTTT3747LysSerAspHisGlyAsnAlaPhePheProAsnSerProSerProPhe123012351240ACACCGCCACCCCCCCAAACCCCCTCTCCTCATGGCACGAGAAGGCAT3795ThrProProProProGlnThrProSerProHisGlyThrArgArgHis124512501255CTGCCATCACCACCACTGACACAGGAGATGGACCTCCATTCCATTGCT3843LeuProSerProProLeuThrGlnGluMetAspLeuHisSerIleAla126012651270GGGCCTCCTGTTCCTCCACGACAAAGCACTTCTCAACTTATCCCCAAA3891GlyProProValProProArgGlnSerThrSerGlnLeuIleProLys1275128012851290CTCCCTCCAAAAACTTACAAAAGGGAGCACACACACCCATCCATGCAT3939LeuProProLysThrTyrLysArgGluHisThrHisProSerMetHis129513001305AGAGATGGACCACCACTGCTGGAGAATGCCCATTCTTCCTGAGTTCCT3987ArgAspGlyProProLeuLeuGluAsnAlaHisSerSer13101315CTAAGCTGGGATAGTTTCCTAGCCCCCAGATCCATTGCTGGCAATGGA4035TGCACTGAACATGCCAGCACTGGGGAGTTCAAATGAGAACTCCAAACACTAAC4088GACTCTACTTCACGATGTAGTATAAGACAATGAGTTTTAACCTACATGGAATTATGGAAT4148AAAATGGTATTCCAGCTTAGAATGTGGAAACTGATTGCACCTGGAAATCACGTGAAGGGA4208CTTTTCTGGCCATTGGGCAGAGTCCTCATATTGTGAAGTGATCTTTATCATTAAAGGGAT4268GGAAAACAGTCTAATGTCCAACAAGCCCATATGTTGACAGTTTTTGTAATTCAAAATATT4328ATGCACTTTTAAAAAATCTTAAACAGGGATCTCCTCCTTTGTTTTCCTTTGCTTTACTCT4388TCTACTTTAGAATATTTTCGTAAAAGTTATTCAGAGGACTGTGAGAAAAGGCTGTGGTAC4448CTGACCTTGTTGAAATCAAGGCCCAGCACTGTACTACAGTCCTGTTTACAGATTATTACA4508GTGATCTGAATGGGTACCGAGGCTTCACCAAAAGAGGTACTTTTTGTTATTGTTATTGTT4568TAAGAATAATTATGCCAATTTAAGAACATCCCCTACCCACCCCCACTCACAAACAAAATG4628TGGTGGTGTTGCCTTTAAACAAAAAATGTCAATGTCATTAACATGATGGAAGAAGAACAT4688TTTAAAACGTAACTGTCAAGTATCATTT4716(2) INFORMATION FOR SEQ ID NO:2:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 1319 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:MetGlnAlaGlnGlnLeuProTyrGluPhePheSerGluGluAsnAla151015ProLysTrpArgGlyLeuLeuValProAlaLeuLysLysValGlnGly202530GlnValHisProThrLeuGluSerAsnAspAspAlaLeuGlnTyrVal354045GluGluLeuIleLeuGlnLeuLeuAsnMetLeuCysGlnAlaGlnPro505560ArgSerAlaSerAspValGluGluArgValGlnLysSerPheProHis65707580ProIleAspLysTrpAlaIleAlaAspAlaGlnSerAlaIleGluLys859095ArgLysArgArgAsnProLeuSerLeuProAlaGluArgIleHisHis100105110LeuLeuArgGluValLeuGlyTyrLysIleAspHisGlnValSerVal115120125TyrIleValAlaValLeuGluTyrIleSerAlaAspIleLeuLysLeu130135140ValGlyAsnTyrValArgAsnIleArgHisTyrGluIleThrLysGln145150155160AspIleLysValAlaMetCysAlaAspLysValLeuMetAspMetPhe165170175HisGlnAspValGluAspIleAsnIleLeuSerLeuThrAspGluGlu180185190ProSerThrSerGlyGluGlnThrTyrTyrAspLeuValLysAlaPhe195200205MetAlaGluIleArgGlnTyrIleArgGluLeuAsnLeuIleIleLys210215220ValPheArgGluProPheValSerAsnSerLysLeuPheSerSerAsn225230235240AspValGluAsnIlePheSerArgIleValAspIleHisGluLeuSer245250255ValLysLeuLeuGlyHisIleGluAspThrValGluMetThrAspGlu260265270GlySerProHisProLeuValGlySerCysPheGluAspLeuAlaGlu275280285GluLeuAlaPheAspProTyrGluSerTyrAlaArgAspIleLeuArg290295300ProGlyPheHisGlyHisPheLeuSerGlnLeuSerLysProGlyAla305310315320AlaLeuTyrLeuGlnSerIleGlyGluGlyPheLysGluAlaValGln325330335TyrValLeuProArgLeuLeuLeuAlaProValTyrHisCysLeuHis340345350TyrPheGluLeuLeuLysGlnLeuGluGluLysSerGluAspGlnGlu355360365AspLysGluCysMetLysGlnAlaIleThrAlaLeuLeuAsnValGln370375380SerGlyMetGluLysIleCysSerLysSerLeuAlaLysArgArgLeu385390395400SerGluSerAlaCysArgPheTyrSerGlnGlnMetLysGlyLysGln405410415LeuAlaIleLysLysMetAsnGluIleGlnLysAsnIleAspGlyTrp420425430GluGlyLysAspIleGlyGlnCysCysAsnGluPheIleMetGluGly435440445ThrLeuThrArgValGlyAlaLysHisGluArgHisIlePheLeuPhe450455460AspGlyLeuMetIleCysCysLysSerAsnHisGlyGlnProArgLeu465470475480ProGlyAlaSerSerAlaGluTyrArgLeuLysGluLysPhePheMet485490495ArgLysValGlnIleAsnAspLysAspAspThrSerGluTyrLysHis500505510AlaPheGluIleIleLeuLysAspGlyAsnSerValIlePheSerAla515520525LysSerAlaGluGluLysAsnAsnTrpMetAlaAlaLeuIleSerLeu530535540GlnTyrArgSerThrLeuGluArgMetLeuAspValThrValLeuGln545550555560GluGluLysGluGluGlnMetArgLeuProSerAlaGluValTyrArg565570575PheAlaGluProAspSerGluGluAsnIleLeuPheGluGluAsnVal580585590GlnProLysAlaGlyIleProIleIleLysAlaGlyThrValLeuLys595600605LeuIleGluArgLeuThrTyrHisMetTyrAlaAspProAsnPheVal610615620ArgThrPheLeuThrThrTyrArgSerPheCysArgProGlnGluLeu625630635640LeuSerLeuLeuIleGluArgPheGluIleProGluProGluProThr645650655GluAlaAspArgIleAlaIleGluAsnGlyAspGlnProLeuSerAla660665670GluLeuLysArgPheArgLysGluTyrIleGlnProValGlnLeuArg675680685ValLeuAsnValCysArgHisTrpValGluHisHisPheTyrAspPhe690695700GluArgAspAlaAspLeuLeuGlnArgMetGluGluPheIleGlyThr705710715720ValArgGlyLysAlaMetLysLysTrpValGluSerIleThrLysIle725730735IleGlnArgLysLysIleAlaArgAspAsnGlyProGlyHisAsnIle740745750ThrPheGlnSerSerProProThrValGluTrpHisIleSerArgPro755760765GlyHisIleGluThrPheAspLeuLeuThrLeuHisProIleGluIle770775780AlaArgGlnLeuThrLeuLeuGluSerAspLeuTyrArgAlaValGln785790795800ProSerGluLeuValGlySerValTrpThrLysGluAspLysGluIle805810815AsnSerProAsnLeuLeuLysMetIleArgHisThrThrAsnLeuThr820825830LeuTrpPheGluLysCysIleValGluThrGluAsnLeuGluGluArg835840845ValAlaValValSerArgIleIleGluIleLeuGlnValPheGlnGlu850855860LeuAsnAsnPheAsnGlyValLeuGluValValSerAlaMetAsnSer865870875880SerProValTyrArgLeuAspHisThrPheGluGlnIleProSerArg885890895GlnLysLysIleLeuGluGluAlaHisGluLeuSerGluAspHisTyr900905910LysLysTyrLeuAlaLysLeuArgSerIleAsnProProCysValPro915920925PhePheGlyIleTyrLeuThrAsnIleLeuLysThrGluGluGlyAsn930935940ProGluValLeuArgArgHisGlyLysGluLeuIleAsnPheSerLys945950955960ArgArgArgValAlaGluIleThrGlyGluIleGlnGlnTyrGlnAsn965970975GlnProTyrCysLeuArgValGluProAspIleLysArgPhePheGlu980985990AsnLeuAsnProMetGlyAsnSerMetGluLysGluPheThrAspTyr99510001005LeuPheAsnLysSerLeuGluIleGluProArgHisProLysProLeu101010151020ProArgPheProLysLysTyrSerTyrProLeuLysSerProGlyVal1025103010351040ArgProSerAsnProArgProGlyThrMetArgHisProThrProLeu104510501055GlnGlnGluProArgLysIleSerTyrSerArgIleProGluSerGlu106010651070ThrGluSerThrAlaSerAlaProAsnSerProArgThrProLeuThr107510801085ProProProAlaSerGlyThrSerSerAsnThrAspValCysSerVal109010951100PheAspSerAspHisSerAlaSerProPheHisSerArgSerAlaSer1105111011151120ValSerSerIleSerLeuSerLysGlyThrAspGluValProValPro112511301135ProProValProProArgArgArgProGluSerAlaProAlaGluSer114011451150SerProSerLysIleMetSerLysHisLeuAspSerProProAlaIle115511601165ProProArgGlnProThrSerLysAlaTyrSerProArgTyrSerIle117011751180SerAspArgThrSerIleSerAspProProGluSerProProLeuLeu1185119011951200ProProArgGluProValArgThrProAspValPheSerSerSerPro120512101215LeuHisLeuGlnProProProLeuGlyLysLysSerAspHisGlyAsn122012251230AlaPhePheProAsnSerProSerProPheThrProProProProGln123512401245ThrProSerProHisGlyThrArgArgHisLeuProSerProProLeu125012551260ThrGlnGluMetAspLeuHisSerIleAlaGlyProProValProPro1265127012751280ArgGlnSerThrSerGlnLeuIleProLysLeuProProLysThrTyr128512901295LysArgGluHisThrHisProSerMetHisArgAspGlyProProLeu130013051310LeuGluAsnAlaHisSerSer1315(2) INFORMATION FOR SEQ ID NO:3:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 5253 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(ix) FEATURE:(A) NAME/KEY: CDS(B) LOCATION: 1..3891(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:CCCACCCTCTCAGCTAATGAAGAGTCTCTCTATTATATTGAAGAACTG48ProThrLeuSerAlaAsnGluGluSerLeuTyrTyrIleGluGluLeu151015ATTTTTCAGCTGCTTAATAAGCTATGCATGGCTCAACCAAGGACTGTT96IlePheGlnLeuLeuAsnLysLeuCysMetAlaGlnProArgThrVal202530CAAGATGTTGAGGAACGAGTTCAAAAGACCTTTCCTCATCCTATTGAT144GlnAspValGluGluArgValGlnLysThrPheProHisProIleAsp354045AAATGGGCAATTGCTGATGCACAATCTGCTATAGAGAAACGAAAACGA192LysTrpAlaIleAlaAspAlaGlnSerAlaIleGluLysArgLysArg505560AGAAATCCTCTCTTACTACCTGTGGACAAAATCCATCCTTCCTTGAAG240ArgAsnProLeuLeuLeuProValAspLysIleHisProSerLeuLys65707580GAAGTTTTGGGGTATAAAGTGGACTACCATGTGTCCCTCTACATTGTG288GluValLeuGlyTyrLysValAspTyrHisValSerLeuTyrIleVal859095GCTGTATTGGAGTATATCTCAGCAGATATTTTGAAATTGGCTGGTAAT336AlaValLeuGluTyrIleSerAlaAspIleLeuLysLeuAlaGlyAsn100105110TATGTTTTTAATATCCGGCATTATGAAATATCTCAGCAAGACATTAAA384TyrValPheAsnIleArgHisTyrGluIleSerGlnGlnAspIleLys115120125GTGTCCATGTGTGCAGATAAGGTTTTGATGGACATGTTCGATCAGGAT432ValSerMetCysAlaAspLysValLeuMetAspMetPheAspGlnAsp130135140GATGATATAGGCTTGGTTTCTCTCTGTGAAGATGAGCCTTGTTCTTCT480AspAspIleGlyLeuValSerLeuCysGluAspGluProCysSerSer145150155160GGTGAGCTAAACTATTATGACCTCGTCAGGACTGAAATTGCAGAAGAA528GlyGluLeuAsnTyrTyrAspLeuValArgThrGluIleAlaGluGlu165170175AGACAGTATCTACGGGAGCTGAATATGATCATTAAAGTGTTCCGGGAA576ArgGlnTyrLeuArgGluLeuAsnMetIleIleLysValPheArgGlu180185190GCCTTTCTCTTGGACAGAAAGTTGTTCAAGCCTTCTGAAATTGAAAAG624AlaPheLeuLeuAspArgLysLeuPheLysProSerGluIleGluLys195200205ATTTTCAGTAACATTTCAGATATACATGAATTGACTGTGAAACTTTTA672IlePheSerAsnIleSerAspIleHisGluLeuThrValLysLeuLeu210215220GGTTTAATTGAAGACACAGTAGAAATGACAGATGAAAGTAGTCCTCAT720GlyLeuIleGluAspThrValGluMetThrAspGluSerSerProHis225230235240CCATTAGCTGGTAGCTGTTTTGAAGATTTAGCAGAGGAGCAAGCGTTT768ProLeuAlaGlySerCysPheGluAspLeuAlaGluGluGlnAlaPhe245250255GATCCCTATGAAACATTATCACAGGACATTCTTGCACCAGAGTTTAAT816AspProTyrGluThrLeuSerGlnAspIleLeuAlaProGluPheAsn260265270GACCACTTCAGCAAGTTGATGGCCAGACCTGCAGTCGCTCTACATTTT864AspHisPheSerLysLeuMetAlaArgProAlaValAlaLeuHisPhe275280285CAGTCCATTGCTGACGGCTTTAAGGAGGCTGTTCGTTATGTCCTTCCA912GlnSerIleAlaAspGlyPheLysGluAlaValArgTyrValLeuPro290295300CGCCTCATGCTGGTTCCCGTGTATCACTGTTGGCATTACTTTGAATTA960ArgLeuMetLeuValProValTyrHisCysTrpHisTyrPheGluLeu305310315320TTAAAGTTGAAGGCATGCAGTGAAGAGCAGGAGGACAAAGAGTGTTTG1008LeuLysLeuLysAlaCysSerGluGluGlnGluAspLysGluCysLeu325330335AATCAGGCTATAACTGCCCTCATGAACCTCCAAGGCAGCATGGACCGC1056AsnGlnAlaIleThrAlaLeuMetAsnLeuGlnGlySerMetAspArg340345350ATTTACAAGCAGCACTCCCCCAGACGCCGGCCTGGGGATCCAGTTTGC1104IleTyrLysGlnHisSerProArgArgArgProGlyAspProValCys355360365CTTTTTTACAATCGTCAATTAAGAAGCAAACACCTGGCTATCAAAAAA1152LeuPheTyrAsnArgGlnLeuArgSerLysHisLeuAlaIleLysLys370375380ATGAATGAAATTCAGAAAAACATAGATGGGTGGGAAGGCAAAGATATC1200MetAsnGluIleGlnLysAsnIleAspGlyTrpGluGlyLysAspIle385390395400GGACAGTGTTGTAATGAGTTCATAATGGAGGGGCCACTGACCAGAATT1248GlyGlnCysCysAsnGluPheIleMetGluGlyProLeuThrArgIle405410415GGTGCTAAACACGAAAGGCATATCTTTCTCTTTGATGGCTTAATGATC1296GlyAlaLysHisGluArgHisIlePheLeuPheAspGlyLeuMetIle420425430AGCTGTAAACCCAATCATGGCCAGACCCGGCTTCCAGGATATAGCAGT1344SerCysLysProAsnHisGlyGlnThrArgLeuProGlyTyrSerSer435440445GCAGAATACAGATTAAAGGAGAAGTTTGTCATGAGGAAAATTCAAATC1392AlaGluTyrArgLeuLysGluLysPheValMetArgLysIleGlnIle450455460TGTGATAAGGAAGACGCCTGTGAGTACAGACATGCTTTTGAATTAGTG1440CysAspLysGluAspAlaCysGluTyrArgHisAlaPheGluLeuVal465470475480TCCAAAGATGAAAACAGTGTAATATTTGCTGCCAAATCAGCTGAAGAG1488SerLysAspGluAsnSerValIlePheAlaAlaLysSerAlaGluGlu485490495AAAAACAACTGGATGGCAGCCCTCATTTCCCTGCACTATCGCAGCACT1536LysAsnAsnTrpMetAlaAlaLeuIleSerLeuHisTyrArgSerThr500505510CTAGACAGAATGCTGGACTCTGTGCTGCTGAAAGAAGAGAATGAGCAG1584LeuAspArgMetLeuAspSerValLeuLeuLysGluGluAsnGluGln515520525CCCCTGAGGCTACCCAGTCCAGATATGTATCGCTTTGTGGTAACAGAC1632ProLeuArgLeuProSerProAspMetTyrArgPheValValThrAsp530535540TCTGAGGAAAACATTGTGTTTGAAGACAACTTGCAAAGCAGAAGTGGG1680SerGluGluAsnIleValPheGluAspAsnLeuGlnSerArgSerGly545550555560ATCCCCATAATTAAAGGAGGCACTGTGGTGAAGTTGATCGAAAGGCTA1728IleProIleIleLysGlyGlyThrValValLysLeuIleGluArgLeu565570575ACATACCACATGTATGCAGATCCCAATTTTGTTCGTACTTTTCTTACT1776ThrTyrHisMetTyrAlaAspProAsnPheValArgThrPheLeuThr580585590ACATATCGTTCATTTTGTAAACCACAGGAATTGCTAAACTTGCTGATA1824ThrTyrArgSerPheCysLysProGlnGluLeuLeuAsnLeuLeuIle595600605GAACGGTTTGAAATTCCAGAACCAGAACCTACTGAGGCAGACAAGCTG1872GluArgPheGluIleProGluProGluProThrGluAlaAspLysLeu610615620GCGTTAGAAAAAGGCGAGCAGCCAATCAGCGCAGATCTGAAAAGATTC1920AlaLeuGluLysGlyGluGlnProIleSerAlaAspLeuLysArgPhe625630635640CGCAAGGAATACGTCCAACCTGTGCAGCTTAGGGTCTTGAATGTCTTT1968ArgLysGluTyrValGlnProValGlnLeuArgValLeuAsnValPhe645650655CGCCACTGGGTTGAGCATCATTATTATGACTTTGAAAGAGACTTGGAA2016ArgHisTrpValGluHisHisTyrTyrAspPheGluArgAspLeuGlu660665670CTGCTTGAAAGACTAGAATCCTTCATTTCAAGTGTAAGAGGGAAAGCC2064LeuLeuGluArgLeuGluSerPheIleSerSerValArgGlyLysAla675680685ATGAAGAAATGGGTAGAATCCATTGCTAAAATAATCAAGAGGAAGAAG2112MetLysLysTrpValGluSerIleAlaLysIleIleLysArgLysLys690695700CAAGCTCAGGCAAATGGAATAAGCCATAATATCACCTTTGAAAGTTCC2160GlnAlaGlnAlaAsnGlyIleSerHisAsnIleThrPheGluSerSer705710715720CCCCCACCAGTGGAATGGCACATCAGTAGAACAGGACAGTTCGAAACA2208ProProProValGluTrpHisIleSerArgThrGlyGlnPheGluThr725730735TTTGACCTTATGACACTTCATCCAATAGAGATCGCACGGCAGCTAACA2256PheAspLeuMetThrLeuHisProIleGluIleAlaArgGlnLeuThr740745750CTTTTGGAATCTGACCTCTACAGGAAAGTCCAGCCCTCTGAACTTGTA2304LeuLeuGluSerAspLeuTyrArgLysValGlnProSerGluLeuVal755760765GGGAGTGTCTGGACCAAAGAAGATAAAGAAATAAATTCTCCAAACTTA2352GlySerValTrpThrLysGluAspLysGluIleAsnSerProAsnLeu770775780TTAAAAATGATTCGCCATACAACAAACCTCACTCTATGGTTTGAGAAA2400LeuLysMetIleArgHisThrThrAsnLeuThrLeuTrpPheGluLys785790795800TGCATTGTGGAAGCAGAAAACTTTGAAGAACGGGTGGCAGTGCTCAGC2448CysIleValGluAlaGluAsnPheGluGluArgValAlaValLeuSer805810815AGAATAGTAGAAATTCTGCAAGTATTTCAAGACTTGAATAATTTCAAT2496ArgIleValGluIleLeuGlnValPheGlnAspLeuAsnAsnPheAsn820825830GGCGTGTTGGAGATAGTGAGTGCAGTCAACTCCGTGTCAGTGTACAGG2544GlyValLeuGluIleValSerAlaValAsnSerValSerValTyrArg835840845CTAGACCACACGTTTGAGGCACTGCAGGAAAGGAAGCGGAGAATTTTG2592LeuAspHisThrPheGluAlaLeuGlnGluArgLysArgArgIleLeu850855860GATGACGCTGTGGAACTAAGTCAGGACCACTTTAAAAAGTACCTAGTA2640AspAspAlaValGluLeuSerGlnAspHisPheLysLysTyrLeuVal865870875880AAACTTAAGTCAATCAATCCGCCTTGTGTGCCTTTTTTTGGAATATAT2688LysLeuLysSerIleAsnProProCysValProPhePheGlyIleTyr885890895TTAACAAATATTCTGAAGACTGAAGAAGGGAACAGTGACTTTCTAAAG2736LeuThrAsnIleLeuLysThrGluGluGlyAsnSerAspPheLeuLys900905910AGGAAAGGGAAAGATTTGATCAATTTCAGTAAGAGGAGGAAAGTGGCT2784ArgLysGlyLysAspLeuIleAsnPheSerLysArgArgLysValAla915920925GAAATAACTGGAGAGATCCAGCAGTATCAGAACCAACCGTACTGCTTA2832GluIleThrGlyGluIleGlnGlnTyrGlnAsnGlnProTyrCysLeu930935940CGGACAGAACCAGAAATGAGGAGATTCTTTGAAAACCTCAACCCCATG2880ArgThrGluProGluMetArgArgPhePheGluAsnLeuAsnProMet945950955960GGAATTTTATCTGAAAAAGAGTTTACAGATTATTTGTTCAACAAATCA2928GlyIleLeuSerGluLysGluPheThrAspTyrLeuPheAsnLysSer965970975TTAGAAATCGAACCCCGAAACTGCAAACAACCACCTCGATTTCCTAGG2976LeuGluIleGluProArgAsnCysLysGlnProProArgPheProArg980985990AAGTCAACCTTTTCCTTAAAATCTCCTGGAATAAGGCCCAATGCTGGC3024LysSerThrPheSerLeuLysSerProGlyIleArgProAsnAlaGly99510001005CGCCATGGCTCTACCTCAGGCACGCTACGAGGTCACCCAACGCCTCTG3072ArgHisGlySerThrSerGlyThrLeuArgGlyHisProThrProLeu101010151020GAAAGAGAGCCTTATAAGATAAGCTTTAGCCGGATCGCTGAGACAGAG3120GluArgGluProTyrLysIleSerPheSerArgIleAlaGluThrGlu1025103010351040CTAGAATCAACAGTGTCTGCACCAACCTCCCCCAACACTCCATCCACC3168LeuGluSerThrValSerAlaProThrSerProAsnThrProSerThr104510501055CCACCAGTGTCTGCTTCTTCAGACCACAGCGTGTTTCTAGATGTGGAC3216ProProValSerAlaSerSerAspHisSerValPheLeuAspValAsp106010651070CTCAATAGCTCCTGTGGCAGCAACACCATCTTTGCTCCAGTCCTCTTG3264LeuAsnSerSerCysGlySerAsnThrIlePheAlaProValLeuLeu107510801085CCACACTCAAAGACTTTCTTCAGCTCATGTGGAAGTTTACACAAACTG3312ProHisSerLysThrPhePheSerSerCysGlySerLeuHisLysLeu109010951100AGTGAAGAGCCACTAATTCCTCCTCCGCTTCCCCCTCGGAAAAAGTTT3360SerGluGluProLeuIleProProProLeuProProArgLysLysPhe1105111011151120GATCATGATGCTCTCAATTCCAAGGGAGCTGTGAAATCTGATGATGAC3408AspHisAspAlaLeuAsnSerLysGlyAlaValLysSerAspAspAsp112511301135CCTCCTGCTATTCCACCAAGACAGCCCCCTCCTCCGAAGGTAAAGCCA3456ProProAlaIleProProArgGlnProProProProLysValLysPro114011451150AGAGTTCCTGTCCTCATGGGTACATTTGATGGGCCTGTGCCCAGTCCA3504ArgValProValLeuMetGlyThrPheAspGlyProValProSerPro115511601165CCTCCACCTCCTCCAAGAGACCCTCTTCCTGATACCCCTCCACCAGTT3552ProProProProProArgAspProLeuProAspThrProProProVal117011751180CCTCTTCGGCCTCCGGAACACTTTATAAACTGTCCATTTAATCTTCAG3600ProLeuArgProProGluHisPheIleAsnCysProPheAsnLeuGln1185119011951200CCGCCTCCACTGGGCCATCCTCACAGAGACCCAGACTGGCTCAGAGAC3648ProProProLeuGlyHisProHisArgAspProAspTrpLeuArgAsp120512101215GTCAGCACGTGTCCTAACTCACCAAGCACTCCTCCCACTACGCCCTCT3696ValSerThrCysProAsnSerProSerThrProProThrThrProSer122012251230CCACGGATTCCACGCAGCTGTCACTTGCTCAGCTCCAGTCACAGCAGC3744ProArgIleProArgSerCysHisLeuLeuSerSerSerHisSerSer123512401245CTTGCTCATCTTCCAGCTCCTCCTGTCCCACCAAGGCAGAATTCAAGC3792LeuAlaHisLeuProAlaProProValProProArgGlnAsnSerSer125012551260CCTCTCTTACCAAAGCTGCCACCAAAGACTTACAAACGGGAGCTTTCC3840ProLeuLeuProLysLeuProProLysThrTyrLysArgGluLeuSer1265127012751280CACCCGCCACTGTATAGACTGCCTCTGCTGGAAAATGCAGAAACTCCT3888HisProProLeuTyrArgLeuProLeuLeuGluAsnAlaGluThrPro128512901295CAATGACCTTGGCCATATGTAGTCATTGACACTGGAATAGTATTTGTAAAGGT3941GlnTTTTAATTTATTCAAAAAGACATAGTATTTTAGTACTTTTTACAGAAATGCTACTGATTA4001AATAAGCTCTTAAGAATTAGTAAACTTGTTGGATGGCAGTGGCCCACAGCTGTAATCCCA4061GCACCCTGGTTGCAGGGGCAGTGGGCTTCAGGGTTTGAGAGCAGCCTGGTCTACAGAGCA4121AGTCCCAGGACAGCCAGGGCTACACAAAAAAACCTTGTCTCAAAAGTCAAAATACAAACA4181AAAGAGAGAAAAGGAAGGAAGGAAAGAATTTGAAATATTAAAATGCAAAAGCCCTTCACC4241ATAGCCCCCAGGTGATCAGCAGCATTGCTTCCTCGGCTCCTCAGTGCTGCCCTAGGCCAG4301TATAAAGACTGTATTGCACTGTAGACTCTGCTAGCAGACGGCACTGAGAGGAGCCAGCGC4361TCGAGGGCCGTTCTCACGCCTGACTGATAGATACCTTTTTAGGGTGATGAACTTACACAG4421ATGCAGAAGATATGGGGTGGTGGTCCTGGGTTTTAACCCTCTGACCGTCTTCTAGCTTCT4481AATTTTGTTTGGTTTTTACATCTAGGAGACTTAGGAATAATACCCGCTGTTCTTAAACTC4541TTTGAGACCATGTCTTAAATGTCAGTATTTGCTGCTGAAGACAAAAACGGAAAATAGAAT4601GAAATAATAGAATGCACTGTGTTTATTATTTTGTTAAAATTATAAACAGTTCTACATAAC4661CTGATTATAGAAGAAGGGCATGTGTTCATTAAGATGTGCCTTTTGTTTTGCAGTGTATGG4721TGTTTAGCTAATCATTGTTTAGCTAATGATTTGCCTATTATTTGGGAAGACAAAATTAAT4781ATGCCATATATGTACAGTTTATTTATATTGTATATATTTAAAGATAATGCTAATAACCTC4841TATAAATGTAAGTGACTTGAGGCCTATAATACAATCTGCTATTTGACTAATTTGTAAGTC4901TGGAACAAAAGTGTCTTATGGCATAAGAACCAACTGCCATTGTCAAACCTATTAACTGTC4961TTTAATCTCGTGTTAACTGAAATTTTTGAAAAGTTTTTCCAGATTAGTAATATTTAAACA5021GAAAATACTTTAAAAAGCTTTATTAAATTTTTTAATCAGACAGGATAAAGCTTTGCCATT5081TGGATACTATCATTCAAAGTGATCAAGGTATGTATGTTATGCTGATAGTGCAGTAGCAGC5141CATTGTAAAGTAGCCAAAAGCCACGTTGTTTATCTACTGGTCTGTGGCCTTTTACTGTGC5201TTTGTATCAGAGTTCTTAACAAGATTAATAAATCACCTCAGTCTTAATTTGT5253(2) INFORMATION FOR SEQ ID NO:4:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 1297 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:ProThrLeuSerAlaAsnGluGluSerLeuTyrTyrIleGluGluLeu151015IlePheGlnLeuLeuAsnLysLeuCysMetAlaGlnProArgThrVal202530GlnAspValGluGluArgValGlnLysThrPheProHisProIleAsp354045LysTrpAlaIleAlaAspAlaGlnSerAlaIleGluLysArgLysArg505560ArgAsnProLeuLeuLeuProValAspLysIleHisProSerLeuLys65707580GluValLeuGlyTyrLysValAspTyrHisValSerLeuTyrIleVal859095AlaValLeuGluTyrIleSerAlaAspIleLeuLysLeuAlaGlyAsn100105110TyrValPheAsnIleArgHisTyrGluIleSerGlnGlnAspIleLys115120125ValSerMetCysAlaAspLysValLeuMetAspMetPheAspGlnAsp130135140AspAspIleGlyLeuValSerLeuCysGluAspGluProCysSerSer145150155160GlyGluLeuAsnTyrTyrAspLeuValArgThrGluIleAlaGluGlu165170175ArgGlnTyrLeuArgGluLeuAsnMetIleIleLysValPheArgGlu180185190AlaPheLeuLeuAspArgLysLeuPheLysProSerGluIleGluLys195200205IlePheSerAsnIleSerAspIleHisGluLeuThrValLysLeuLeu210215220GlyLeuIleGluAspThrValGluMetThrAspGluSerSerProHis225230235240ProLeuAlaGlySerCysPheGluAspLeuAlaGluGluGlnAlaPhe245250255AspProTyrGluThrLeuSerGlnAspIleLeuAlaProGluPheAsn260265270AspHisPheSerLysLeuMetAlaArgProAlaValAlaLeuHisPhe275280285GlnSerIleAlaAspGlyPheLysGluAlaValArgTyrValLeuPro290295300ArgLeuMetLeuValProValTyrHisCysTrpHisTyrPheGluLeu305310315320LeuLysLeuLysAlaCysSerGluGluGlnGluAspLysGluCysLeu325330335AsnGlnAlaIleThrAlaLeuMetAsnLeuGlnGlySerMetAspArg340345350IleTyrLysGlnHisSerProArgArgArgProGlyAspProValCys355360365LeuPheTyrAsnArgGlnLeuArgSerLysHisLeuAlaIleLysLys370375380MetAsnGluIleGlnLysAsnIleAspGlyTrpGluGlyLysAspIle385390395400GlyGlnCysCysAsnGluPheIleMetGluGlyProLeuThrArgIle405410415GlyAlaLysHisGluArgHisIlePheLeuPheAspGlyLeuMetIle420425430SerCysLysProAsnHisGlyGlnThrArgLeuProGlyTyrSerSer435440445AlaGluTyrArgLeuLysGluLysPheValMetArgLysIleGlnIle450455460CysAspLysGluAspAlaCysGluTyrArgHisAlaPheGluLeuVal465470475480SerLysAspGluAsnSerValIlePheAlaAlaLysSerAlaGluGlu485490495LysAsnAsnTrpMetAlaAlaLeuIleSerLeuHisTyrArgSerThr500505510LeuAspArgMetLeuAspSerValLeuLeuLysGluGluAsnGluGln515520525ProLeuArgLeuProSerProAspMetTyrArgPheValValThrAsp530535540SerGluGluAsnIleValPheGluAspAsnLeuGlnSerArgSerGly545550555560IleProIleIleLysGlyGlyThrValValLysLeuIleGluArgLeu565570575ThrTyrHisMetTyrAlaAspProAsnPheValArgThrPheLeuThr580585590ThrTyrArgSerPheCysLysProGlnGluLeuLeuAsnLeuLeuIle595600605GluArgPheGluIleProGluProGluProThrGluAlaAspLysLeu610615620AlaLeuGluLysGlyGluGlnProIleSerAlaAspLeuLysArgPhe625630635640ArgLysGluTyrValGlnProValGlnLeuArgValLeuAsnValPhe645650655ArgHisTrpValGluHisHisTyrTyrAspPheGluArgAspLeuGlu660665670LeuLeuGluArgLeuGluSerPheIleSerSerValArgGlyLysAla675680685MetLysLysTrpValGluSerIleAlaLysIleIleLysArgLysLys690695700GlnAlaGlnAlaAsnGlyIleSerHisAsnIleThrPheGluSerSer705710715720ProProProValGluTrpHisIleSerArgThrGlyGlnPheGluThr725730735PheAspLeuMetThrLeuHisProIleGluIleAlaArgGlnLeuThr740745750LeuLeuGluSerAspLeuTyrArgLysValGlnProSerGluLeuVal755760765GlySerValTrpThrLysGluAspLysGluIleAsnSerProAsnLeu770775780LeuLysMetIleArgHisThrThrAsnLeuThrLeuTrpPheGluLys785790795800CysIleValGluAlaGluAsnPheGluGluArgValAlaValLeuSer805810815ArgIleValGluIleLeuGlnValPheGlnAspLeuAsnAsnPheAsn820825830GlyValLeuGluIleValSerAlaValAsnSerValSerValTyrArg835840845LeuAspHisThrPheGluAlaLeuGlnGluArgLysArgArgIleLeu850855860AspAspAlaValGluLeuSerGlnAspHisPheLysLysTyrLeuVal865870875880LysLeuLysSerIleAsnProProCysValProPhePheGlyIleTyr885890895LeuThrAsnIleLeuLysThrGluGluGlyAsnSerAspPheLeuLys900905910ArgLysGlyLysAspLeuIleAsnPheSerLysArgArgLysValAla915920925GluIleThrGlyGluIleGlnGlnTyrGlnAsnGlnProTyrCysLeu930935940ArgThrGluProGluMetArgArgPhePheGluAsnLeuAsnProMet945950955960GlyIleLeuSerGluLysGluPheThrAspTyrLeuPheAsnLysSer965970975LeuGluIleGluProArgAsnCysLysGlnProProArgPheProArg980985990LysSerThrPheSerLeuLysSerProGlyIleArgProAsnAlaGly99510001005ArgHisGlySerThrSerGlyThrLeuArgGlyHisProThrProLeu101010151020GluArgGluProTyrLysIleSerPheSerArgIleAlaGluThrGlu1025103010351040LeuGluSerThrValSerAlaProThrSerProAsnThrProSerThr104510501055ProProValSerAlaSerSerAspHisSerValPheLeuAspValAsp106010651070LeuAsnSerSerCysGlySerAsnThrIlePheAlaProValLeuLeu107510801085ProHisSerLysThrPhePheSerSerCysGlySerLeuHisLysLeu109010951100SerGluGluProLeuIleProProProLeuProProArgLysLysPhe1105111011151120AspHisAspAlaLeuAsnSerLysGlyAlaValLysSerAspAspAsp112511301135ProProAlaIleProProArgGlnProProProProLysValLysPro114011451150ArgValProValLeuMetGlyThrPheAspGlyProValProSerPro115511601165ProProProProProArgAspProLeuProAspThrProProProVal117011751180ProLeuArgProProGluHisPheIleAsnCysProPheAsnLeuGln1185119011951200ProProProLeuGlyHisProHisArgAspProAspTrpLeuArgAsp120512101215ValSerThrCysProAsnSerProSerThrProProThrThrProSer122012251230ProArgIleProArgSerCysHisLeuLeuSerSerSerHisSerSer123512401245LeuAlaHisLeuProAlaProProValProProArgGlnAsnSerSer125012551260ProLeuLeuProLysLeuProProLysThrTyrLysArgGluLeuSer1265127012751280HisProProLeuTyrArgLeuProLeuLeuGluAsnAlaGluThrPro128512901295Gln(2) INFORMATION FOR SEQ ID NO:5:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 1572 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:MetPheSerGlyProSerGlyHisAlaHisThrIleSerTyrGlyGly151015GlyIleGlyLeuGlyThrGlyGlyGlyGlyGlySerGlyGlySerGly202530SerGlySerGlnGlyGlyGlyGlyGlyIleGlyIleGlyGlyGlyGly354045ValAlaGlyLeuGlnAspCysAspGlyTyrAspPheThrLysCysGlu505560AsnAlaAlaArgTrpArgGlyLeuPheThrProSerLeuLysLysVal65707580LeuGluGlnValHisProArgValThrAlaLysGluAspAlaLeuLeu859095TyrValGluLysLeuCysLeuArgLeuLeuAlaMetLeuCysAlaLys100105110ProLeuProHisSerValGlnAspValGluGluLysValAsnLysSer115120125PheProAlaProIleAspGlnTrpAlaLeuAsnGluAlaLysGluVal130135140IleAsnSerLysLysArgLysSerValLeuProThrGluLysValHis145150155160ThrLeuLeuGlnLysAspValLeuGlnTyrLysIleAspSerSerVal165170175SerAlaPheLeuValAlaValLeuGluTyrIleSerAlaAspIleLeu180185190LysMetAlaGlyAspTyrValIleLysIleAlaHisCysGluIleThr195200205LysGluAspIleGluValValMetAsnAlaAspArgValLeuMetAsp210215220MetLeuAsnGlnSerGluAlaHisIleLeuProSerProLeuSerLeu225230235240ProAlaGlnArgAlaSerAlaThrTyrGluGluThrValLysGluLeu245250255IleHisAspGluLysGlnTyrGlnArgAspLeuHisMetIleIleArg260265270ValPheArgGluGluLeuValLysIleValSerAspProArgGluLeu275280285GluProIlePheSerAsnIleMetAspIleTyrGluValThrValThr290295300LeuLeuGlySerLeuGluAspValIleGluMetSerGlnGluGlnSer305310315320AlaProCysValGlySerCysPheGluGluLeuAlaGluAlaGluGlu325330335PheAspValTyrLysLysTyrAlaTyrAspValThrSerGlnAlaSer340345350ArgAspAlaLeuAsnAsnLeuLeuSerLysProGlyAlaSerSerLeu355360365ThrThrAlaGlyHisGlyPheArgAspAlaValLysTyrTyrLeuPro370375380LysLeuLeuLeuValProIleCysHisAlaPheValTyrPheAspTyr385390395400IleLysHisLeuLysAspLeuSerSerSerGlnAspAspIleGluSer405410415PheGluGlnValGlnGlyLeuLeuHisProLeuHisCysAspLeuGlu420425430LysValMetAlaSerLeuSerLysGluArgGlnValProValSerGly435440445ArgValArgArgGlnLeuAlaIleGluArgThrArgGluLeuGlnMet450455460LysValGluHisTrpGluAspLysAspValGlyGlnAsnCysAsnGlu465470475480PheIleArgGluAspSerLeuSerLysLeuGlySerGlyLysArgIle485490495TrpSerGluArgLysValPheLeuPheAspGlyLeuMetValLeuCys500505510LysAlaAsnThrLysLysGlnThrProSerAlaGlyAlaThrAlaTyr515520525AspTyrArgLeuLysGluLysTyrPheMetArgArgValAspIleAsn530535540AspArgProAspSerAspAspLeuLysAsnSerPheGluLeuAlaPro545550555560ArgMetGlnProProIleValLeuThrAlaLysAsnAlaGlnHisLys565570575HisAspTrpMetAlaAspLeuLeuMetValIleThrLysSerMetLeu580585590AspArgHisLeuAspSerIleLeuGlnAspIleGluArgLysHisPro595600605LeuArgMetProSerProGluIleTyrLysPheAlaValProAspSer610615620GlyAspAsnIleValLeuGluGluArgGluSerAlaGlyValProMet625630635640IleLysGlyAlaThrLeuCysLysLeuIleGluArgLeuThrTyrHis645650655IleTyrAlaAspProThrPheValArgThrPheLeuThrThrTyrArg660665670TyrPheCysSerProGlnGlnLeuLeuGlnLeuLeuValGluArgPhe675680685AsnIleProAspProSerLeuValTyrGlnAspThrGlyThrAlaGly690695700AlaGlyGlyMetGlyGlyValGlyGlyAspLysGluHisLysAsnSer705710715720HisArgGluAspTrpLysArgTyrArgLysGluTyrValGlnProVal725730735GlnPheArgValLeuAsnValLeuArgHisTrpValAspHisHisPhe740745750TyrAspPheGluLysAspProMetLeuLeuGluLysLeuLeuAsnPhe755760765LeuGluHisValAsnGlyLysSerMetArgLysTrpValAspSerVal770775780LeuLysIleValGlnArgLysAsnGluGlnGluLysSerAsnLysLys785790795800IleValTyrAlaTyrGlyHisAspProProProIleGluHisHisLeu805810815SerValProAsnAspGluIleThrLeuLeuThrLeuHisProLeuGlu820825830LeuAlaArgGlnLeuThrLeuLeuGluPheGluMetTyrLysAsnVal835840845LysProSerGluLeuValGlySerProTrpThrLysLysAspLysGlu850855860ValLysSerProAsnLeuLeuLysIleMetLysHisThrThrAsnVal865870875880ThrArgTrpIleGluLysSerIleThrGluAlaGluAsnTyrGluGlu885890895ArgLeuAlaIleMetGlnArgAlaIleGluValMetMetValMetLeu900905910GluLeuAsnAsnPheAsnGlyIleLeuSerIleValAlaAlaMetGly915920925ThrAlaSerValTyrArgLeuArgTrpThrPheGlnGlyLeuProGlu930935940ArgTyrArgLysPheLeuGluGluCysArgGluLeuSerAspAspHis945950955960LeuLysLysTyrGlnGluArgLeuArgSerIleAsnProProCysVal965970975ProPhePheGlyArgTyrLeuThrAsnIleLeuHisLeuGluGluGly980985990AsnProAspLeuLeuAlaAsnThrGluLeuIleAsnPheSerLysArg99510001005ArgLysValAlaGluIleIleGlyGluIleGlnGlnTyrGlnAsnGln101010151020ProTyrCysLeuAsnGluGluSerThrIleArgGlnPhePheGluGln1025103010351040LeuAspProPheAsnGlyLeuSerAspLysGlnMetSerAspTyrLeu104510501055TyrAsnGluSerLeuArgIleGluProArgGlyCysLysThrValPro106010651070LysPheProArgLysTrpProHisIleProLeuLysSerProGlyIle107510801085LysProArgArgGlnAsnGlnThrAsnSerSerSerLysLeuSerAsn109010951100SerThrSerSerValAlaAlaAlaAlaAlaAlaSerSerThrAlaThr1105111011151120SerIleAlaThrAlaSerAlaProSerLeuHisAlaSerSerIleMet112511301135AspAlaProThrAlaAlaAlaAlaAsnAlaGlySerGlyThrLeuAla114011451150GlyGluGlnSerProGlnHisAsnProHisAlaPheSerValPheAla115511601165ProValIleIleProGluArgAsnThrSerSerTrpSerGlyThrPro117011751180GlnHisThrArgThrAspGlnAsnAsnGlyGluValSerValProAla1185119011951200ProHisLeuProLysLysProGlyAlaHisValTrpAlaAsnAsnAsn120512101215SerThrLeuAlaSerAlaSerAlaMetAspValValPheSerProAla122012251230LeuProGluHisLeuProProGlnSerLeuProAspSerAsnProPhe123512401245AlaSerAspThrGluAlaProProSerProLeuProLysLeuValVal125012551260SerProArgHisGluThrGlyAsnArgSerProPheHisGlyArgMet1265127012751280GlnAsnSerProThrHisSerThrAlaSerThrValThrLeuThrGly128512901295MetSerThrSerGlyGlyGluGluPheCysAlaGlyGlyPheTyrPhe130013051310AsnSerAlaHisGlnGlyGlnProGlyAlaValProIleSerProHis131513201325ValAsnValProMetAlaThrAsnMetGluTyrArgAlaValProPro133013351340ProLeuProProArgArgLysGluArgThrGluSerCysAlaAspMet1345135013551360AlaGlnLysArgGlnAlaProAspAlaProThrLeuProProArgAsp136513701375GlyGluLeuSerProProProIleProProArgLeuAsnHisSerThr138013851390GlyIleSerTyrLeuArgGlnSerHisGlyLysSerLysGluPheVal139514001405GlyAsnSerSerLeuLeuLeuProAsnThrSerSerIleMetIleArg141014151420ArgAsnSerAlaIleGluLysArgAlaAlaAlaThrSerGlnProAsn1425143014351440GlnAlaAlaAlaGlyProIleSerThrThrLeuValThrValSerGln144514501455AlaValAlaThrAspGluProLeuAlaAlaThrAspLeuAlaSerGly146014651470LysLeuLeuAspAspHisIleThrAlaAspThrArgHisValAlaHis147514801585ValSerGlnHisSerGlnProSerGlyGlyGluHisValGluGlnLeu149014951500CysProProThrAlaAsnAlaAlaAlaAlaAlaAlaAlaAspAlaSer1505151015151520SerAspLeuLeuAlaAlaProSerThrSerCysHisProSerAlaAla152515301535ProSerAlaProAlaProPheGluSerAspAlaValAlaLeuValPro154015451550GlnGlyValLeuSerAspCysHisGluProArgGlyHisThrGlnThr155515601565SerThrLysThr1570(2) INFORMATION FOR SEQ ID NO:6:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 1336 amino acids(B) TYPE: amino acids(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:MetLeuValSerHisLeuIleLeuProLysLysGlnHisProAlaGly151015ThrMetGlnAlaGlnGlnLeuProTyrGluPhePheSerGluGluAsn202530AlaProLysTrpArgGlyLeuLeuValProAlaLeuLysLysValGln354045GlyGlnValHisProThrLeuGluSerAsnAspAspAlaLeuGlnTyr505560ValGluGluLeuIleLeuGlnLeuLeuAsnMetLeuCysGlnAlaGln65707580ProArgSerAlaSerAspValGluGluArgValGlnLysSerPhePro859095HisProIleAspLysTrpAlaIleAlaAspAlaGlnSerAlaIleGlu100105110LysArgLysArgArgAsnProLeuSerLeuProAlaGluArgIleHis115120125HisLeuLeuArgGluValLeuGlyTyrLysIleAspHisGlnValSer130135140ValTyrIleValAlaValLeuGluTyrIleSerAlaAspIleLeuLys145150155160LeuValGlyAsnTyrValArgAsnIleArgHisTyrGluIleThrLys165170175GlnAspIleLysValAlaMetCysAlaAspLysValLeuMetAspMet180185190PheHisGlnAspValGluAspIleAsnIleLeuSerLeuThrAspGlu195200205GluProSerThrSerGlyGluGlnThrTyrTyrAspLeuValLysAla210215220PheMetAlaGluIleArgGlnTyrIleArgGluLeuAsnLeuIleIle225230235240LysValPheArgGluProPheValSerAsnSerLysLeuPheSerSer245250255AsnAspValGluAsnIlePheSerArgIleValAspIleHisGluLeu260265270SerValLysLeuLeuGlyHisIleGluAspThrValGluMetThrAsp275280285GluGlySerProHisProLeuValGlySerCysPheGluAspLeuAla290295300GluGluLeuAlaPheAspProTyrGluSerTyrAlaArgAspIleLeu305310315320ArgProGlyPheHisGlyHisPheLeuSerGlnLeuSerLysProGly325330335AlaAlaLeuTyrLeuGlnSerIleGlyGluGlyPheLysGluAlaVal340345350GlnTyrValLeuProArgLeuLeuLeuAlaProValTyrHisCysLeu355360365HisTyrPheGluLeuLeuLysGlnLeuGluGluLysSerGluAspGln370375380GluAspLysGluCysMetLysGlnAlaIleThrAlaLeuLeuAsnVal385390395400GlnSerGlyMetGluLysIleCysSerLysSerLeuAlaLysArgArg405410415LeuSerGluSerAlaCysArgPheTyrSerGlnGlnMetLysGlyLys420425430GlnLeuAlaIleLysLysMetAsnGluIleGlnLysAsnIleAspGly435440445TrpGluGlyLysAspIleGlyGlnCysCysAsnGluPheIleMetGlu450455460GlyThrLeuThrArgValGlyAlaLysHisGluArgHisIlePheLeu465470475480PheAspGlyLeuMetIleCysCysLysSerAsnHisGlyGlnProArg485490495LeuProGlyAlaSerSerAlaGluTyrArgLeuLysGluLysPhePhe500505510MetArgLysValGlnIleAsnAspLysAspAspThrSerGluTyrLys515520525HisAlaPheGluIleIleLeuLysAspGlyAsnSerValIlePheSer530535540AlaLysSerAlaGluGluLysAsnAsnTrpMetAlaAlaLeuIleSer545550555560LeuGlnTyrArgSerThrLeuGluArgMetLeuAspValThrValLeu565570575GlnGluGluLysGluGluGlnMetArgLeuProSerAlaGluValTyr580585590ArgPheAlaGluProAspSerGluGluAsnIleLeuPheGluGluAsn595600605ValGlnProLysAlaGlyIleProIleIleLysAlaGlyThrValLeu610615620LysLeuIleGluArgLeuThrTyrHisMetTyrAlaAspProAsnPhe625630635640ValArgThrPheLeuThrThrTyrArgSerPheCysArgProGlnGlu645650655LeuLeuSerLeuLeuIleGluArgPheGluIleProGluProGluPro660665670ThrGluAlaAspArgIleAlaIleGluAsnGlyAspGlnProLeuSer675680685AlaGluLeuLysArgPheArgLysGluTyrIleGlnProValGlnLeu690695700ArgValLeuAsnValCysArgHisTrpValGluHisHisPheTyrAsp705710715720PheGluArgAspAlaAspLeuLeuGlnArgMetGluGluPheIleGly725730735ThrValArgGlyLysAlaMetLysLysTrpValGluSerIleThrLys740745750IleIleGlnArgLysLysIleAlaArgAspAsnGlyProGlyHisAsn755760765IleThrPheGlnSerSerProProThrValGluTrpHisIleSerArg770775780ProGlyHisIleGluThrPheAspLeuLeuThrLeuHisProIleGlu785790795800IleAlaArgGlnLeuThrLeuLeuGluSerAspLeuTyrArgAlaVal805810815GlnProSerGluLeuValGlySerValTrpThrLysGluAspLysGlu820825830IleAsnSerProAsnLeuLeuLysMetIleArgHisThrThrAsnLeu835840845ThrLeuTrpPheGluLysCysIleValGluThrGluAsnLeuGluGlu850855860ArgValAlaValValSerArgIleIleGluIleLeuGlnValPheGln865870875880GluLeuAsnAsnPheAsnGlyValLeuGluValValSerAlaMetAsn885890895SerSerProValTyrArgLeuAspHisThrPheGluGlnIleProSer900905910ArgGlnLysLysIleLeuGluGluAlaHisGluLeuSerGluAspHis915920925TyrLysLysTyrLeuAlaLysLeuArgSerIleAsnProProCysVal930935940ProPhePheGlyIleTyrLeuThrAsnIleLeuLysThrGluGluGly945950955960AsnProGluValLeuArgArgHisGlyLysGluLeuIleAsnPheSer965970975LysArgArgArgValAlaGluIleThrGlyGluIleGlnGlnTyrGln980985990AsnGlnProTyrCysLeuArgValGluProAspIleLysArgPhePhe99510001005GluAsnLeuAsnProMetGlyAsnSerMetGluLysGluPheThrAsp101010151020TyrLeuPheAsnLysSerLeuGluIleGluProArgHisProLysPro1025103010351040LeuProArgPheProLysLysTyrSerTyrProLeuLysSerProGly104510501055ValArgProSerAsnProArgProGlyThrMetArgHisProThrPro106010651070LeuGlnGlnGluProArgLysIleSerTyrSerArgIleProGluSer107510801085GluThrGluSerThrAlaSerAlaProAsnSerProArgThrProLeu109010951100ThrProProProAlaSerGlyThrSerSerAsnThrAspValCysSer1105111011151120ValPheAspSerAspHisSerAlaSerProPheHisSerArgSerAla112511301135SerValSerSerIleSerLeuSerLysGlyThrAspGluValProVal114011451150ProProProValProProArgArgArgProGluSerAlaProAlaGlu115511601165SerSerProSerLysIleMetSerLysHisLeuAspSerProProAla117011751180IleProProArgGlnProThrSerLysAlaTyrSerProArgTyrSer1185119011951200IleSerAspArgThrSerIleSerAspProProGluSerProProLeu120512101215LeuProProArgGluProValArgThrProAspValPheSerSerSer122012251230ProLeuHisLeuGlnProProProLeuGlyLysLysSerAspHisGly123512401245AsnAlaPhePheProAsnSerProSerProPheThrProProProPro125012551260GlnThrProSerProHisGlyThrArgArgHisLeuProSerProPro1265127012751280LeuThrGlnGluMetAspLeuHisSerIleAlaGlyProProValPro128512901295ProArgGlnSerThrSerGlnLeuIleProLysLeuProProLysThr130013051310TyrLysArgGluHisThrHisProSerMetHisArgAspGlyProPro131513201325LeuLeuGluAsnAlaHisSerSer13301335(2) INFORMATION FOR SEQ ID NO:7:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 108 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:TGTGTTCTAGTTGGGTGCAAGGTCTAGCCTAAGGCAATGCTTGTCTCC48CysValLeuValGlyCysLysValProLysAlaMetLeuValSer151015CACCTCATCCTGCCAAGGAAGCAGCACCCCGCGGGCACCATGCAGGCG96HisLeuIleLeuProArgLysGlnHisProAlaGlyThrMetGlnAla202530CAGCAGCTGCCT108GlnGlnLeuPro35(2) INFORMATION FOR SEQ ID NO:8:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 57 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA(xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:GCCCCTCCGCCCGCCCCGAGGCGCCCCGCGGGCACCATGCAGGCGCAG48AlaProProProAlaProArgArgProAlaGlyThrMetGlnAlaGln151015CAGCTGCCT57GlnLeuPro(2) INFORMATION FOR SEQ ID NO:9:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 430 amino acids(B) TYPE: amino acids(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:IleLysGlyAlaThrLeuCysLysLeuIleGluArgLeuThrTyrHis151015IleTyrAlaAspProThrPheValArgThrPheLeuThrThrTyrArg202530TyrPheCysSerProGlnGlnLeuLeuGlnLeuLeuValGluArgPhe354045AsnIleProAspProSerLeuValTyrGlnAspThrGlyThrAlaGly505560AlaGlyGlyMetGlyGlyValGlyGlyAspLysGluHisLysAsnSer65707580HisArgGluAspTrpLysArgTyrArgLysGluTyrValGlnProVal859095GlnPheArgValLeuAsnValLeuArgHisTrpValAspHisHisPhe100105110TyrAspPheGluLysAspProMetLeuLeuGluLysLeuLeuAsnPhe115120125LeuGluHisValAsnGlyLysSerMetArgLysTrpValAspSerVal130135140LeuLysIleValGlnArgLysAsnGluGlnGluLysSerAsnLysLys145150155160IleValTyrAlaTyrGlyHisAspProProProIleGluHisHisLeu165170175SerValProAsnAspGluIleThrLeuLeuThrLeuHisProLeuGlu180185190LeuAlaArgGlnLeuThrLeuLeuGluPheGluMetTyrLysAsnVal195200205LysProSerGluLeuValGlySerProTrpThrLysLysAspLysGlu210215220ValLysSerProAsnLeuLeuLysIleMetLysHisThrThrAsnVal225230235240ThrArgTrpIleGluLysSerIleThrGluAlaGluAsnTyrGluGlu245250255ArgLeuAlaIleMetGlnArgAlaIleGluValMetMetValMetLeu260265270GluLeuAsnAsnPheAsnGlyIleLeuSerIleValAlaAlaMetGly275280285ThrAlaSerValTyrArgLeuArgTrpThrPheGlnGlyLeuProGlu290295300ArgTyrArgLysPheLeuGluGluCysArgGluLeuSerAspAspHis305310315320LeuLysLysTyrGlnGluArgLeuArgSerIleAsnProProCysVal325330335ProPhePheGlyArgTyrLeuThrAsnIleLeuHisLeuGluGluGly340345350AsnProAspLeuLeuAlaAsnThrGluLeuIleAsnPheSerLysArg355360365ArgLysValAlaGluIleIleGlyGluIleGlnGlnTyrGlnAsnGln370375380ProTyrCysLeuAsnGluGluSerThrIleArgGlnPhePheGluGln385390395400LeuAspProPheAsnGlyLeuSerAspLysGlnMetSerAspTyrLeu405410415TyrAsnGluSerLeuArgIleGluProArgGlyCysLysThr420425430(2) INFORMATION FOR SEQ ID NO:10:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 423 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:IleLysAlaGlyThrValLeuLysLeuIleGluArgLeuThrTyr151015HisMetTyrAlaAspProAsnPheValArgThrPheLeuThrThr202530TyrArgSerPheCysArgProGlnGluLeuLeuSerLeuLeuIle354045GluArgPheGluIleProGluProGluProThrGluAlaAspArg505560IleAlaIleGluAsnGlyAspGlnProLeuSerAlaGluLeuLys657075ArgPheArgLysGluTyrIleGlnProValGlnLeuArgValLeu808590AsnValCysArgHisTrpValGluHisHisPheTyrAspPheGlu95100105ArgAspAlaAspLeuLeuGlnArgMetGluGluPheIleGlyThr110115120ValArgGlyLysAlaMetLysLysTrpValGluSerIleThrLys125130135IleIleGlnArgLysLysIleAlaArgAspAsnGlyProGlyHis140145150AsnIleThrPheGlnSerSerProProThrValGluTrpHisIle155160165SerArgProGlyHisIleGluThrPheAspLeuLeuThrLeuHis170175180ProIleGluIleAlaArgGlnLeuThrLeuLeuGluSerAspLeu185190195TyrArgAlaValGlnProSerGluLeuValGlySerValTrpThr200205210LysGluAspLysGluIleAsnSerProAsnLeuLeuLysMetIle215220225ArgHisThrThrAsnLeuThrLeuTrpPheGluLysCysIleVal230235240GluThrGluAsnLeuGluGluArgValAlaValValSerArgIle245250255IleGluIleLeuGlnValPheGlnGluLeuAsnAsnPheAsnGly260265270ValLeuGluValValSerAlaMetAsnSerSerProValTyrArg275280285LeuAspHisThrPheGluGlnIleProSerArgGlnLysLysIle290295300LeuGluGluAlaHisGluLeuSerGluAspHisTyrLysLysTyr305310315LeuAlaLysLeuArgSerIleAsnProProCysValProPhePhe320325330GlyIleTyrLeuThrAsnIleLeuLysThrGluGluGlyAsnPro335340345GluValLeuArgArgHisGlyLysGluLeuIleAsnPheSerLys350355360ArgArgArgValAlaGluIleThrGlyGluIleGlnGlnTyrGln365370375AsnGlnProTyrCysLeuArgValGluProAspIleLysArgPhe380385390PheGluAsnLeuAsnProMetGlyAsnSerMetGluLysGluPhe395400405ThrAspTyrLeuPheAsnLysSerLeuGluIleGluProArgHis410415420ProLysPro(2) INFORMATION FOR SEQ ID NO:11:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 423 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:IleLysGlyGlyThrValValLysLeuIleGluArgLeuThrTyr151015HisMetTyrAlaAspProAsnPheValArgThrPheLeuThrThr202530TyrArgSerPheCysLysProGlnGluLeuLeuAsnLeuLeuIle354045GluArgPheGluIleProGluProGluProThrGluAlaAspLys505560LeuAlaLeuGluLysGlyGluGlnProIleSerAlaAspLeuLys657075ArgPheArgLysGluTyrValGlnProValGlnLeuArgValLeu808590AsnValPheArgHisTrpValGluHisHisTyrTyrAspPheGlu95100105ArgAspLeuGluLeuLeuGluArgLeuGluSerPheIleSerSer110115120ValArgGlyLysAlaMetLysLysTrpValGluSerIleAlaLys125130135IleIleLysArgLysLysGlnAlaGlnAlaAsnGlyIleSerHis140145150AsnIleThrPheGluSerSerProProProValGluTrpHisIle155160165SerArgThrGlyGlnPheGluThrPheAspLeuMetThrLeuHis170175180ProIleGluIleAlaArgGlnLeuThrLeuLeuGluSerAspLeu185190195TyrArgLysValGlnProSerGluLeuValGlySerValTrpThr200205210LysGluAspLysGluIleAsnSerProAsnLeuLeuLysMetIle215220225ArgHisThrThrAsnLeuThrLeuTrpPheGluLysCysIleVal230235240GluAlaGluAsnPheGluGluArgValAlaValLeuSerArgIle245250255ValGluIleLeuGlnValPheGlnAspLeuAsnAsnPheAsnGly260265270ValLeuGluIleValSerAlaValAsnSerValSerValTyrArg275280285LeuAspHisThrPheGluAlaLeuGlnGluArgLysArgArgIle290295300LeuAspAspAlaValGluLeuSerGlnAspHisPheLysLysTyr305310315LeuValLysLeuLysSerIleAsnProProCysValProPhePhe320325330GlyIleTyrLeuThrAsnIleLeuLysThrGluGluGlyAsnSer335340345AspPheLeuLysArgLysGlyLysAspLeuIleAsnPheSerLys350355360ArgArgLysValAlaGluIleThrGlyGluIleGlnGlnTyrGln365370375AsnGlnProTyrCysLeuArgThrGluProGluMetArgArgPhe380385390PheGluAsnLeuAsnProMetGlyIleLeuSerGluLysGluPhe395400405ThrAspTyrLeuPheAsnLysSerLeuGluIleGluProArgAsn410415420CysLysGln(2) INFORMATION FOR SEQ ID NO:12:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 426 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:IleArgGlyGlyThrLysGluAlaLeuIleGluHisLeuThrSer151015HisGluLeuValAspAlaAlaPheAsnValThrMetLeuIleThr202530PheArgSerIleLeuThrThrArgGluPhePheTyrAlaLeuIle354045TyrArgTyrAsnLeuTyrProProGluGlyLeuSerTyrAspAsp505560TyrAsnIleTrpIleGluLysLysSerAsnProIleLysCysArg657075ValValAsnIleMetArgThrPheLeuThrGlnTyrTrpThrArg808590AsnTyrTyrGluProGlyIleProLeuIleLeuAsnPheAlaLys95100105MetValValSerGluLysIleProGlyAlaGluAspLeuLeuGln110115120LysIleAsnGluLysLeuIleAsnGluAsnGluLysGluProVal125130135AspProLysGlnGlnAspSerValSerAlaValValGlnThrThr140145150LysArgAspAsnLysSerProIleHisMetSerSerSerSerLeu155160165ProSerSerAlaSerSerAlaPhePheArgLeuLysLysLeuLys170175180LeuLeuAspIleAspProTyrThrTyrAlaThrGlnLeuThrVal185190195LeuGluHisAspLeuTyrLeuArgIleThrMetPheGluCysLeu200205210AspArgAlaTrpGlyThrLysTyrCysAsnMetGlyGlySerPro215220225AsnIleThrLysPheIleAlaAsnAlaAsnThrLeuThrAsnPhe230235240ValSerHisThrIleValLysGlnAlaAspValLysThrArgSer245250255LysLeuThrGlnTyrPheValThrValAlaGlnHisCysLysGlu260265270LeuAsnAsnPheSerSerMetThrAlaIleValSerAlaLeuTyr275280285SerSerProIleTyrArgLeuLysLysThrTrpAspLeuValSer290295300ThrGluSerLysAspLeuLeuLysAsnLeuAsnAsnLeuMetAsp305310315SerLysArgAsnPheValLysTyrArgGluLeuLeuArgSerVal320325330ThrAspValAlaCysValProPhePheGlyValTyrLeuSerAsp335340345LeuThrPheThrPheValGlyAsnProAspPheLeuHisAsnSer350355360ThrAsnIleIleAsnPheSerLysArgThrLysIleAlaAsnIle365370375ValGluGluIleIleSerPheLysArgPheHisTyrLysLeuLys380385390ArgLeuAspAspIleGlnThrValIleGluAlaSerLeuGluAsn395400405ValProHisIleGluLysGlnTyrGlnLeuSerLeuGlnValGlu410415420ProProSerGlyAsnThr425(2) INFORMATION FOR SEQ ID NO:13:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 418 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:IleLysGlyGlySerLysHisAlaLeuIleSerTyrLeuThrAsp151015AsnGluLysLysAspLeuPhePheAsnIleThrPheLeuIleThr202530PheArgSerIlePheThrThrThrGluPheLeuSerTyrLeuIle354045SerGlnTyrAsnLeuAspProProGluAspLeuCysPheGluGlu505560TyrAsnGluTrpValThrLysLysLeuIleProValLysCysArg657075ValValGluIleMetThrThrPhePheLysGlnTyrTrpPhePro808590GlyTyrAspGluProAspLeuAlaThrLeuAsnLeuAspTyrPhe95100105AlaGlnValAlaIleLysGluAsnIleThrGlySerValGluLeu110115120LeuLysGluValAsnGlnLysPheLysLeuGlyAsnIleGlnGlu125130135AlaThrAlaProMetLysThrLeuAspGlnGlnIleCysGlnAsp140145150HisTyrSerGlyThrLeuTyrSerThrThrGluSerIleLeuAla155160165ValAspProValLeuPheAlaThrGlnLeuThrIleLeuGluHis170175180GluIleTyrCysGluIleThrThrPheAspCysLeuGlnLysIle185190195TrpLysAsnLysTyrThrLysSerTyrGlyAlaSerProGlyLeu200205210AsnGluPheIleSerPheAlaAsnLysLeuThrAsnPheIleSer215220225TyrSerValValLysGluAlaAspLysSerLysArgAlaLysLeu230235240LeuSerHisPheIlePheIleAlaGluTyrCysArgLysPheAsn245250255AsnPheSerSerMetThrAspIleIleSerAlaLeuTyrSerSer260265270ProIleTyrArgLeuGluLysThrTrpGlnAlaValIleProGln275280285ThrArgAspLeuLeuGlnSerLeuAsnLysLeuMetAspProLys290295300LysAsnPheIleAsnTyrArgAsnGluLeuLysSerLeuHisSer305310315AlaProCysValProPhePheGlyValTyrLeuSerAspLeuThr320325330PheThrAspSerGlyAsnProAspTyrLeuValLeuGluHisGly335340345LeuLysGlyValHisAspGluLysLysTyrIleAsnPheAsnLys350355360ArgSerArgLeuValAspIleLeuGlnGluIleIleTyrPheLys365370375LysThrHisTyrAspPheThrLysAspArgThrValIleGluCys380385390IleSerAsnSerLeuGluAsnIleProHisIleGluLysGlnTyr395400405GlnLeuSerLeuIleIleGluProLysProArgLysLys410415(2) INFORMATION FOR SEQ ID NO:14:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 402 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:IleLysThrAlaThrLeuValPheIleIleAsnTyrLeuLeuArg151015ThrAspIleAspSerThrPhePheThrThrIlePheLeuAsnThr202530TyrAlaSerMetIleSerSerSerAspLeuPheSerIleLeuGly354045AlaHisPheArgPheIleCysSerLeuAsnPheGlyLysIleSer505560PheIleSerHisGluPheTyrArgValSerLysArgPheLeuAsp657075IleLeuLeuIleTrpPheGluSerTyrLeuValGluGluLeuAsp808590AsnSerLysSerIlePhePheLeuPheLysIleTyrLysValPhe95100105GluValPheValValProHisPheAlaSerAlaGluGluLeuLeu110115120HisSerLeuSerHisLeuLeuHisHisProSerThrLysArgSer125130135HisLysMetLeuGluGlyLysGluLeuSerGlnGluLeuGluAsp140145150LeuSerLeuHisAsnSerProAspProIleIleTyrLysAspGlu155160165LeuValLeuLeuLeuProProArgGluIleAlaLysGlnLeuCys170175180IleLeuGluPheGlnSerPheSerHisIleSerArgIleGlnPhe185190195LeuThrLysIleTrpAspGluLeuAsnArgPheSerProLysGlu200205210LysThrSerThrPheTyrLeuSerAsnHisLeuValAsnPheVal215220225ThrGluThrIleValGlnGluGluGluProArgArgArgThrAsn230235240ValLeuAlaTyrPheIleGlnValCysAspTyrLeuArgGluLeu245250255AsnAsnPheAlaSerLeuPheSerIleIleSerAlaLeuAsnSer260265270SerProIleHisArgLeuArgLysThrTrpAlaAsnLeuAsnSer275280285LysThrLeuAlaSerPheGluLeuLeuAsnAsnLeuThrGluAla290295300ArgLysAsnPheSerAsnTyrArgAspCysLeuGluAsnCysVal305310315LeuProCysValProPheLeuGlyValTyrPheThrAspLeuThr320325330PheLeuLysThrGlyAsnLysAspAsnPheGlnAsnMetIleAsn335340345PheAspLysArgThrLysValThrArgIleLeuAsnGluIleLys350355360LysPheGlnSerValGlyTyrMetPheAsnProIleAsnGluVal365370375GlnGluLeuLeuAsnGluValIleSerArgGluArgAsnThrAsn380385390AsnIleTyrGlnArgSerLeuThrValGluProArg395400(2) INFORMATION FOR SEQ ID NO:15:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 362 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:ProSerProPheAspSerAlaAsnLeuLeuLeuAsnPheArgAsp151015TrpThrThrAspAsnAlaLeuLeuGlnGluLeuLeuLeuSerTyr202530ProThrIleAsnLysAsnLysHisLysAsnHisSerValProArg354045LeuIleGlnIleTrpValGluSerTyrTrpGlnAspSerGluThr505560ThrLeuLysAspIleLeuAsnPheTrpTyrSerHisLeuAlaGlu657075TyrTyrGluTyrGlnGluLeuPheAlaAspIleValGlnLeuPhe808590IleAsnLysLysArgThrArgGlnLeuLysIleHisTyrIleGly95100105LeuThrAspLysGluIleGluGluAsnLysProProLeuAspTyr110115120GluAsnLeuPheLeuGlnTyrGluIleAspLysThrAsnAlaAsn125130135AspGluLeuCysGlyAlaThrAspLeuSerAspLeuLeuPheGln140145150TrpLysGlnGlyGluProLeuGluValGluAlaPheAlaLeuAsn155160165ValSerProTrpSerLeuAlaLysThrLeuThrLeuLeuGluSer170175180SerLeuTyrLeuAspIleGluThrIleGluPheThrArgHisPhe185190195LysHisAsnAspThrThrIleAspSerValPheThrLeuSerAsn200205210GlnLeuSerSerTyrValLeuGluThrThrLeuGlnGlnThrHis215220225ThrIleSerTyrTrpLeuGlnValAlaLeuAlaCysLeuTyrLeu230235240ArgAsnLeuAsnSerLeuAlaSerIleIleThrSerLeuGlnAsn245250255HisSerIleGluArgLeuSerLeuProIleAspValLysSerAsp260265270HisLeuPheGlnArgLeuLysValValValHisProAsnAsnAsn275280285TyrAsnValTyrArgArgThrIleLysHisIlePheHisSerGln290295300LeuProCysValProPheThrSerLeuLeuIleArgAspIleThr305310315PheIleArgAspGlyAsnAspThrPheThrLysAspGlyAsnAsn320325330ValAsnMetGlnLysPheAsnGlnIleThrLysIleValAlaPhe335340345AlaGlnTyrLeuGlnGlnLysGlnTyrGluAspIleHisCysSer350355360AsnThr__________________________________________________________________________

DNA molecules encoding murine son of sevenless (mSOS) gene and mSOS polypeptides

Information

Patent Number

Date Filed

Date Issued

Inventors

Original Assignees

Examiners

Agents

CPC

US Classifications

Field of Search

US

International Classifications

Abstract

Description

Claims

Priority Claims (1)

PCT Information

Non-Patent Literature Citations (2)

Entry
Bonfini et al. (Jan. 1992) Science 255:603-606.
Bowtell (Jul. 1992) Proc. Natl. Acad. Sci. USA 89:6511-6515.