Claims
- 1. An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of
- (a) nucleotide #1 through nucleotide #387 of figure I,
- (b) nucleotide #356 through nucleotide #1543 of figure II,
- (c) Nucleotide #402 through nucleotide #1626 of figure III,
- (d) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a), (b), and (c); and
- (e) sequences which
- (1) hybridize to any of sequences (a), (b), (c), or (d) under stringent hybridization conditions; and
- (2) encode a protein characterized by the ability to induce the formation of bone and/or cartilage.
- 2. A vector comprising a DNA sequence of claim 1 in operative association with an expression control sequence for said DNA sequence.
- 3. A host cell transformed with the vector of claim 2 said host cells selected from the group consisting of mammalian, bacterial, yeast and insect cells.
- 4. A method for producing an osteoinductive protein, said method comprising the steps of
- (a) culturing in a suitable culture medium said transformed host cell of claim 3; and
- (b) isolating and purifying said osteoinductive protein from said culture medium.
- 5. The host cell of claim 4 wherein said host cell is a mammalian cell.
- 6. The host cell of claim 4 wherein said cells are selected from the group consisting of bacterial, yeast and insect cells.
- 7. A mammalian host cell transformed with the vector of claim 2.
Parent Case Info
This application is a continuation-in-part of Ser. Nos. 28,285 filed Mar. 20, 1987 now abandoned; 943,332 filed Dec. 17, 1986 now abandoned; and 880,776 filed July 1, 1986 now abandoned. This application also claims priority of PCT/US887/01537 filed June 30, 1987.
US Referenced Citations (2)
Non-Patent Literature Citations (2)
Entry |
Maniatis, T. et al., Molecular Cloning, A Laboratory Manual, Cold Spring Hobar Laboratory, Csh, N.Y. (1982). |
Suggs, S. V. et al., Proc. Natl. Acad. Sci., U.S.A., 501 78, pp. 6613-6617 (1981). |
Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
28285 |
Mar 1987 |
|
Parent |
943332 |
Dec 1986 |
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Parent |
880776 |
Jul 1986 |
|