Claims
- 1. A nucleic acid molecule comprising (a) the nucleotide sequence of SEQ ID NO: 1; or (b) a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO: 2 when the nucleotide sequence is translated according to the codon usage of Candida albicans.
- 2. The nucleic acid molecule according to claim 1 which further comprises a promoter operably linked to the nucleotide sequence.
- 3. The nucleic acid molecule according to claim 2 which further comprises a terminator operably linked to the nucleotide sequence.
- 4. The nucleic acid molecule according to claim 2 or 3, where the promoter or terminator is that of the CaACT1 gene.
- 5. The nucleic acid molecule according to claim 2 or 3, wherein the promoter or terminator is that of the CaPCK1 gene.
- 6. The nucleic acid molecule according to claim 1 further comprising a nucleotide sequence encoding a negative selectable marker.
- 7. The nucleic acid molecule according to claim 6 wherein the negative selectable marker is thymidine kinase.
- 8. The nucleic acid molecule according to claim 1, 2, or 3 further comprising a first nucleotide sequence and a second nucleotide sequence, wherein the first and second nucleotide sequences flank both ends of the nucleic acid molecule, and are hybridizable separately to different non-contiguous regions of a chromosome of Candida albicans or Saccharomyces cerevisiae.
- 9. The nucleic acid molecule of claim 8, wherein the first and second nucleotide sequences are hybridizable to regions of a yeast chromosome that are contiguous with a gene selected from the group consisting of CaKRE1, CaWSC3, and CaYHR036W.
- 10. A vector comprising the nucleic acid molecule of claim 1, 2, 3, or 8.
- 11. An expression vector comprising the nucleic acid of claim 2, wherein a protein displaying the activity of streptothricin acetyltransferase is expressible in Candida albicans.
- 12. A kit comprising the vector of claim 10.
- 13. A yeast cell comprising the nucleic acid molecule of 1, 2, or 3 wherein the yeast cell is selected from the group consisting of Candida albicans and Saccharomyces cerevisiae.
- 14. A yeast cell comprising a streptothricin acetyltransferase comprising the amino acid sequence of SEQ ID NO: 2 wherein the yeast cell is selected from the group consisting of Candida albicans and Saccharomyces cerevisiae.
- 15. A method of culturing yeast cells in the presence of nourseothricin comprising: (a) introducing the nucleic acid molecule of claim 2 into the yeast cells; and (b) culturing the yeast cells such that the nucleotide sequence of claim 2 is expressed in the yeast cells, wherein the yeast cells are cells of Candida albicans or Saccharomyces cerevisae.
- 16. A method of identifying yeast cells comprising the nucleic acid molecule of claim 2, said method comprising:
(a) introducing the nucleic acid molecule of claim 2 into the yeast cells; and (b) culturing the yeast cells under conditions suitable for growth and in the presence of nourseothricin for a time sufficient for the expression of the nucleotide sequence of claim 2, such that the yeast cells that contain the nucleic acid molecule grow faster than yeast cells that do not contain or express the nucleic acid molecule, thereby allowing the yeast cells that contain the nucleic acid molecule to be identified, wherein the yeast cells are cells of Candida albicans or Saccharomyces cerevisae.
- 17. A method for enriching yeast cells that comprise a first nucleic acid molecule, said method comprising:
(a) introducing a mixture of nucleic acid molecules into the yeast cells, said mixture comprising the first nucleic acid molecule and the nucleic acid molecule of claim 2; (b) culturing the yeast cells under growth conditions and in the presence of nourseothricin for a time sufficient for the expression of the nucleotide sequence of claim 2, such that the yeast cells that contain the nucleic acid molecule of claim 2 grow faster than yeast cells that do not contain or express the nucleic acid molecule of claim 2, thereby allowing the yeast cells that comprise the nucleic acid molecule of claim 2 to be identified; and (c) recovering the yeast cells that comprise the nucleic acid molecule of claim 2, wherein said recovered yeast cells are enriched for yeast cells that comprise the first nucleic acid molecule, wherein the yeast cells are cells of Candida albicans or Saccharomyces cerevisae.
- 18. A method of treatment of a disease in a subject caused by an infection by a Candida albicans, said method comprising administering to the subject in need of such treatment a pharmaceutical composition comprising a pharmaceutically acceptable carrier and nourseothricin.
- 19. A method of inhibiting the growth of Candida albicans cells comprising contacting Candida albicans cells with a composition comprising an effective amount of nourseothricin.
- 20. A method of inhibiting the growth of Saccharomyces cerevisiae cells comprising contacting Saccharomyces cerevisiae cells with a composition comprising an effective amount of nourseothricin.
- 21. A method of preventing or reducing contamination of an object by a fungus comprising contacting the object with a composition comprising an effective amount of nourseothricin.
- 22. A method of preventing or reducing formation on a surface of a biofilm comprising Candida albicans, said method comprising contacting the surface with a composition comprising an effective amount of nourseothricin.
- 23. A culture medium suitable for growth of Candida albicans comprising nourseothricin.
- 24. A culture medium suitable for growth of Saccharomyces cerevisae comprising nourseothricin.
Parent Case Info
[0001] This application claims priority to the U.S. provisional application No. 60/183,462, filed Feb. 18, 2000, which is incorporated herein by reference in its entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60183462 |
Feb 2000 |
US |