Patent Application
20250057159
This application claims the benefit of priority from Chinese Patent Application No. 202410645603.0, filed on May 23, 2024. The content of the aforementioned application, including any intervening amendments made thereto, is incorporated herein by reference in its entirety.
This application relates to dust mite attractants, and more particularly to a dust mite attractant and its microcapsule preparation method.
Dust mites are primary allergens commonly found in household dust, and approximately 10% to 20% of people worldwide are allergic to them. They will trigger type I allergic hypersensitivity reaction, such as allergic asthma, allergic rhinitis, dermatitis, and chronic urticaria. They widely breed in household items (e.g., carpets, mattresses, and sofa) and daily necessities as well as food items, such that it is difficult to control and treat them. As people increasingly prioritize their health and living environments, especially those affected by dust mite allergies and their families, there has been a growing emphasis on the prevention and control of dust mites. Attractants, as a technological approach to pest management, are also gaining popularity.
There are currently a wide variety of products available on the market for controlling dust mites, most of which have a complex action mechanism. Therefore, there is an urgent need to develop an efficient and safe attractant, which could attract the dust mites in the living environment to facilitate the subsequent control and treatment, so as to prevent the allergic reaction caused thereby.
In order to diversify the dust mite control approaches in the prior art, the present disclosure provides a dust mite attractant and a method for preparing a microcapsule thereof, which enable the efficient, safe, and easy control of dust mites.
Technical solutions of the present disclosure are described as follows.
In a first aspect, this application provides a dust mite attractant, comprising:
In some embodiments, a weight ratio of the neryl propionate to the geranyl formate to the methyl eugenol to the geraniol to the terpene is 10:4:4:1:1.
In some embodiments, the dust mite attractant is in a form of solid or liquid.
In a second aspect, this application provides a method for preparing a microcapsule of the dust mite attractant, comprising:
In some embodiments, in step (4), the emulsification is performed sequentially by shear emulsification at 10,000 rpm for 8 min and ultrasonic emulsification under stirring for 10 min.
In some embodiments, in step (5), the reaction is performed at a rotation speed of 600 rpm for 2.5 h.
Compared to the prior art, the present disclosure has the following beneficial effects.
(1) Any one of the five components in the attractant of the present disclosure exhibits an attractive effect on dust mites, particularly neryl propionate and geranyl formate, which demonstrate excellent efficacy in attracting dust mites. Compared with the existing mite prevention and control products with complex action mechanism, the present disclosure is easy to operate. Specifically, attractant components contain mite aggregation pheromones, which can produce a scent to attract the mites to assemble, thereby facilitating the treatment of mites and reducing the number of dust mites in the living environment.
(2) Compared to those compositions composed of one, two, three or fourth selected from the group consisting of neryl propionate, geranyl formate, methyl eugenol, geraniol, the terpene, the attractant composed of the five selected components in the present disclosure exhibits a significantly-enhanced attracting efficacy. Additionally, these five substances have a slow volatile rate, and are non-toxic, all of which are components of natural or synthetic fragrances. These substances are easily soluble, and are easy to prepare. Furthermore, they all possess a pleasant fragrance such that they can improve the production, storage, and application environments.
(3) It has been experimentally demonstrated that the attractant provided by the present disclosure exhibits a significant attracting effect on Dermatophagoides pteronyssinus and Dermatophagoides farinae (two common species of dust mites). This attractant can effectively attract dust mites within 48 h and 96 h. In addition, different preparation forms (liquid and solid) exhibit a similar attracting effect. Therefore, the attractant of the present disclosure has superior efficacy, excellent long-acting characteristic, and diverse forms, such that it can adapt to various application environments.
(4) The present disclosure also provides a method for preparing the dust mite attractant microcapsule, in which the liquid attractant is uniformly mixed with isophorone diisocyanate to form the oil phase. Based on good solubility of individual components of the attractant in an organic solvent, the prepared oil phase is stable, in which individual components are not susceptible to decomposition, and reaction with each other.
The technical solutions of the present disclosure will be described in further detail below with reference to the accompanying drawings and embodiments.
To facilitate the understanding of the present disclosure, a more comprehensive description will be provided below with reference to the accompanying drawings, in which several embodiments of the disclosure are illustrated. However, the present disclosure can be implemented in various forms and is not limited to the embodiments described herein. Rather, the provision of these embodiments is intended to illustrate the technical solutions disclosed in the present disclosure more fully and completely.
Provided herein was a liquid dust mite attractant, which included neryl propionate, geranyl formate, methyl eugenol, geraniol and a terpene in a weight ratio of 10:4:4:1:1.
The liquid dust mite attractant was prepared as follows.
The neryl propionate, the geranyl formate, the methyl eugenol, the geraniol, and the terpene (all liquid materials) were mixed uniformly according to the above weight ratio to obtain the liquid attractant, which was stored in the dark for use.
The dust mite attractant provided herein was different from that provided in Example 1 merely in the compounding ratio of individual ingredients. Specifically, in this example, the weight ratio of the neryl propionate to the geranyl formate to the methyl eugenol to the geraniol to the terpene was 1:1:5:5:5.
The dust mite attractant provided herein was different from that provided in Example 1 merely in the compounding ratio of individual ingredients. Specifically, in this example, the weight ratio of the neryl propionate to the geranyl formate to the methyl eugenol to the geraniol to the terpene was 5:4:8:8:6.
The dust mite attractant provided herein was different from that provided in Example 1 merely in the compounding ratio of individual ingredients. Specifically, in this example, the weight ratio of the neryl propionate to the geranyl formate to the methyl eugenol to the geraniol to the terpene was 5:3:3:4:3.
The dust mite attractant provided herein was different from that provided in Example 1 merely in the compounding ratio of individual ingredients. Specifically, in this example, the weight ratio of the neryl propionate to the geranyl formate to the methyl eugenol to the geraniol to the terpene was 1:1:1:1:1.
Provided herein was a dust mite attractant in the form of microcapsule, which included neryl propionate, geranyl formate, methyl eugenol, geraniol and a terpene in a weight ratio of 10:4:4:1:1.
The dust mite attractant microcapsule was prepared as follows.
1.5 g of gum arabic and 100 mL of deionized water were mixed uniformly to obtain the aqueous phase.
2.5 g of the liquid attractant obtained in EXAMPLE 1 and 3.7 g of isophorone diisocyanate were mixed uniformly to obtain the oil phase.
1.25 g of tetraethylenepentamine and 30 g of deionized water were mixed uniformly to obtain the tetraethylenepentamine solution.
The aqueous phase obtained in step (1) was slowly added with the oil phase obtained in step (2), and subjected to high-speed shear emulsification at 10,000 rpm for 8 min, and ultrasonic emulsification under stirring for 10 min to obtain an emulsified mixture.
Step (5) The emulsified mixture was transferred to a three-necked flask, which was placed in an oil bath at 35° C. and 600 rpm, and dropwise added with the tetraethylenepentamine solution obtained in step (3). After that, the oil bath temperature was increased to 65° C., and the reaction was performed at such temperature for 2.5 h to obtain the solid attractant (microcapsule) for dust mites.
The solid attractant prepared in Example 6 for dust mites was observed under a microscope, as shown in
The testing process was performed based on a long-range olfactometer (1100 mm×100 mm×80 mm). As shown in
The liquid attractant was dissolved in n-hexane and then added to the Petri dish in the test area. 100 mg of yeast feed was added to the Petri dish coated with paraffin oil in the blank control area. After that, 100 mg of a mite-containing culture was placed on a filter paper (60 mm) at the center of the release area. After 48 h, the mites in the Petri dishes from the test and blank control areas were counted, respectively. This experiment was performed in triplicate, and the results were averaged and shown in Table 1 and
It could be seen from Table 1 and
The testing process was performed also based on the long-range olfactometer (1100 mm×100 mm×80 mm). The solid attractant was added to the Petri dish within the test area, and 100 mg of yeast feed was added to the Petri dish coated with paraffin oil in the blank control area. After that, 100 mg of a mite culture was placed on a filter paper (60 mm) at the center of the release area. After 48 h and 96 h, the mites in the Petri dishes from the test and blank control areas were counted, respectively. This experiment was performed in triplicate, and the results were averaged and shown in Table 2 and
It could be demonstrated by Table 2 and
The testing process was performed based on the same long-range olfactometer (1100 mm×100 mm×80 mm). The liquid dust mite attractant was dissolved in n-hexane and then added to the Petri dish in the test area, while the solid dust mite attractant was added to the Petri dish in the blank control area. After that, 100 mg of a mite (Dermatophagoides farinae)-containing culture was placed on a filter paper (60 mm) at the center of the release area. After 48 h and 96 h, the mites in the Petri dishes from the test and blank control areas were counted, respectively. This experiment was performed in triplicate, and the results were averaged and shown in Table 3 and
The Dermatophagoides farinae-containing culture was replaced with a Dermatophagoides pteronyssinus-containing culture to investigate the attracting effect on Dermatophagoides pteronyssinus according to the above process. The results were shown in Table 4 and
As shown in Tables 3-4, and
The embodiments described above are merely illustrative of the present disclosure, and are not intended to limit the scope of the present disclosure. It should be understood that various changes or substitutions made by those of ordinary skill in the art without departing from the spirit of the present disclosure shall fall within the scope of the present disclosure defined by the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202410645603.0 | May 2024 | CN | national |
