Claims
- 1. A method of stimulating cell growth comprising the step of contacting said cell with an EGFL6 polypeptide or a variant thereof having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 24.
- 2. A method of stimulating cell growth comprising the step of contacting said cell with an EGFL6 polypeptide fragment of SEQ ID NO: 24 wherein the fragment lacks a C-terminal portion of the EGFL6 polypeptide.
- 3. The method of claims 1 or 2 wherein the cell is selected from the group consisting of kidney cells, heart cells, brain cells, neurons and adrenal cells.
- 4. The method of claim 2 wherein the fragment comprises amino acids 1 to 377 of SEQ ID NO: 24.
- 5. The method of claim 2 wherein the fragment comprises amino acids 22 to 377 of SEQ ID NO: 24.
- 6. A fusion protein comprising the EGFL6 polypeptide of SEQ ID NO: 24 or a fragment thereof which exhibits growth promoting activity and a heterologous polypeptide.
- 7. The fusion protein of claim 6, wherein the EGFL6 polypeptide fragment comprises amino acids 1 to 377 of SEQ ID NO: 24.
- 8. The fusion protein of claim 6, wherein the EGFL6 polypeptide fragment comprises amino acids 22 to 377 of SEQ ID NO: 24.
- 9. The fusion protein of any one of claims 6, 7 and 8, wherein the heterologous polypeptide is a human Fc polypeptide.
- 10. A polypeptide fragment of SEQ ID NO: 24, wherein the fragment lacks a C-terminal portion of EGFL6 polypeptide and exhibits growth promoting activity.
- 11. The polypeptide fragment of claim 9 wherein the fragment lacks the C-terminal amino acids 378 to 553 of SEQ ID NO: 24.
- 12. The polypeptide fragment of claim 9 wherein the fragment further lacks amino acids 1 to 22 of SEQ ID NO: 24.
1. RELATED APPLICATIONS
[0001] This patent application is a continuation-in-part of U.S. patent application Ser. No. 09/981,649 filed Oct. 15, 2001 which is a continuation-in-part of U.S. patent application Ser. No. 09/687,860 filed Oct. 13, 2000 which is a continuation-in-part of U.S. patent Ser. No. 09/363,316 filed Jul. 28, 1999 which is a continuation-in-part of U.S. patent application Ser. No. 09/249,697 filed Feb. 12, 1999 which is a continuation-in-part of U.S. patent application Ser. No. 08/968,200 filed Nov. 22, 1997. All of these applications are herein incorporated by reference in their entirety.
Continuation in Parts (5)
|
Number |
Date |
Country |
Parent |
09981649 |
Oct 2001 |
US |
Child |
10124986 |
Apr 2002 |
US |
Parent |
09687860 |
Oct 2000 |
US |
Child |
09981649 |
Oct 2001 |
US |
Parent |
09363316 |
Jul 1999 |
US |
Child |
09687860 |
Oct 2000 |
US |
Parent |
09249697 |
Feb 1999 |
US |
Child |
09363316 |
Jul 1999 |
US |
Parent |
08968800 |
Nov 1997 |
US |
Child |
09249697 |
Feb 1999 |
US |