ENCODED MICROPARTICLES

Abstract

An encoded microparticle including an optical substrate comprising a material that permits light to propagate therethrough. The optical substrate has an elongated body that extends in a direction along a central axis. The optical substrate includes an outer region that extends about the central axis. The encoded microparticle also includes an optically detectable code that is disposed within the optical substrate and extends along the central axis. The outer region surrounds the optically detectable code about the central axis. The optically detectable code is readable when the light propagates through the outer region and is at least one of reflected or filtered by the optically detectable code. Said at least one of reflected or filtered light propagates through the outer region to be detected for reading the optically detectable code.

Description

BACKGROUND OF INVENTION

1. Technical Field

The present invention relates to a method and apparatus for fabricating an optical identification element; and more particularly to a method and apparatus for fabricating a holographic optical identification element using a lithographic technique, as well as the holographic optical identification element itself.

SUMMARY OF INVENTION

The present invention provides a new and Unique method for fabricating an optical identification element, wherein a removable plate or substrate having a photosensitive material fabricated on is provided, one or more gratings are written on the photosensitive material, then lines are etched to create one or more separate optical identification elements.

The one or more gratings may be written by exposing the photosensitive material to ultraviolet (UV) light.

The lines may be etched to create the one or more separate optical identification elements by photolithography to define/create the same.

The one or more separate optical identification element are planar elements.

The optical identification element may take the form of a holographic optical identification element having one of the following geometric shapes, such as a plate, a bar, a brick, a disc, a slab, etc.

The method according to the present invention enables many possible options, geometries, sizes, photosensitive materials in relation to the overall fabrication of an optical identification element.

The present invention also includes the possibility of using a surface relief grating, a densification grating, cover slips, or borosilicate.

The scope of the invention is also intended to include the apparatus for fabricating an optical identification element consistent with the description of the aforementioned method, including a combination of devices for performing the steps described above, as well as an optical identification element that results from the steps of the method or process shown and described herein.

One advantage of the present invention is that conventional technology may be used to fabricate an optical identification element with a high level of flexibility.

In effect, the present invention potentially adds a whole new dimension to existing biochip technology.

BRIEF DESCRIPTION OF THE DRAWINGS

The drawings, which are not drawn to scale, include the following:

FIG. 1 is a diagram of steps for fabricating a optical identification element according to the present invention.

FIG. 2 is a diagram of a partially etched substrate according to the present invention.

FIG. 3 is a block diagram of an optical arrangement for fabricating an optical identification element according to the present invention.

FIG. 4 is a diagram of an optical identification element according to the present invention.

FIG. 5 is a side view of an optical identification element.

FIG. 6 is a perspective view of an optical identification element having a grating that is smaller than the substrate.

FIGS. 7( a)-(c) show images of digital codes on a CCD camera.

DETAILED DESCRIPTION OF THE INVENTION

FIG. 1 shows steps 1-4 for performing a method for fabricating an optical identification element 20 (see also FIG. 4) according to the present invention.

In step 1, a removable plate or substrate 10 having a photosensitive material 10 fabricated thereon. Suitable photosensitive materials are known in the art, and the scope of the invention is not intended to be limited to any particular kind either now known or later developed in the future. The plate or substrate 10 may take the form of many different medium or material, including, but not limited to, an optical medium or material, although the scope of the invention is also intended to include other materials for the substrate now known or later developed in the future.

In step 2, one or more gratings 13 (best shown in FIGS. 2 and 4) are written on the photosensitive material 12, for example, by exposing the photosensitive material 12 to ultraviolet (UV) light 14, although the scope of the invention is also intended to include using other grating writing, techniques either now known or later developed in the future.

In step 3, one or more lines 16 are etched or formed to create and form one or more separate optical identification elements that are generally indicated as 18 in FIG. 1 using photolithography to define/create the same, although the scope of the invention is also intended to include using other etching techniques either now known or later developed in the future.

In step 4, the etching process in step 3 results in the formation of the one or more separate optical identification elements 20. In this case, the elements 18 are removed or separated from the substrate 10 by the etching process. Alternatively, the elements 18 may be removed or separated from the substrate 10 by exposing them in a suitable solution and form the one or more optical identification elements 20. Such a suitable solution is known in the art, and the scope of the invention is not intended to be limited to any particular type or kind thereof. The one or more separate optical identification element 20 take the form of planar elements, as distinguished from optical fiber, optical filaments, or the like that are known in the art.

Moreover, the scope of the invention is intended to include the optical identification element 20 taking the form of a holographic optical identification element or other suitable optical identification element having an interference pattern reproduced from a pattern of interference produced by a split coherent beam of radiation (as a laser) either now known or later developed in the future, or by any of the techniques described in copending U.S. patent application. Ser. Nos. 10/661,234 or 10/661,116, and other related cases referenced herein. Moreover, the optical identification elements 20 may take the geometric form of one or more planar objects, including plates, bars, bricks, discs, slabs, chips, or other suitable planar geometric shape and/or dimensionality now known or later developed in the future, including those described in U.S. patent application Ser. No. 10/661,234 and other patent applications referenced herein.

Alternative Technique

The present invention also provides an alternative format micro “chip” assay technique relating to code reading via embedded collocated gratings, as follows:

Grating Orientation:

The scope of the invention is intended to include at least the following grating orientation techniques shown by way of example in FIG. 2:

1) Writing grating codes 13a, 13b, 13c, 13d1, 13d2 at multiple axes across each disc or element 18—where a single axis reader always may be used to pick up one code.

2) Adding orientation ‘marker’ 15 to one or more discs or elements 18, such as by adding one or more of the following:

a) Magnetic material for self-alignment;

b) Birefringence; or

c) Fluorescence to determine alignment/orientation.

3) Putting each “bit” in along a different axis and use a spinning readout system (e.g. each bit assessed).

The Optical Arrangement or Apparatus

The scope of the invention is also intended to include an optical arrangement or apparatus for fabricating an optical identification element consistent with the description of the aforementioned method, including a combination of devices for performing the steps described above. For example, FIG. 3 shows the optical arrangement or apparatus generally indicated as 30 for fabricating such an optical identification element 20, including the combination of a means or device 32 for providing a removable plate or substrate 10 having the photosensitive material 12 fabricated thereon; a means or device 34 for writing one or more gratings 13, 13a, 13b, 13c, 13d1, 13d2 (see FIG. 2) on the photosensitive material 12; and a means or device 36 for separating and creating the one or more separate optical identification elements 20, including by, e.g., etching the lines 16 on the photosensitive material 12.

The Optical Identification Element 20

FIG. 4 shows, by way of example, the optical identification element 20 in greater detail that results from the steps of the method or process shown in FIG. 1, and/or the optical arrangement shown in FIG. 3. The scope of the invention is also intended to include the optical identification element itself made by the method or process set forth above, including, but not limited to, a holographic optical identification element made from the lithographic technique described herein. The present invention may be used to create the encoded elements consistent with that described in copending. U.S. patent application Ser. No. 10/661,234, filed 12 Sep. 2003 (CyVera Docket No. CV-0038A) and the other patent applications referenced herein, which are incorporated herein by reference in their entirety.

Referring to FIG. 5, an optical identification element 108 comprises a known optical substrate 110, having an optical diffraction grating 112 disposed (or written, impressed, embedded, imprinted, etched, grown, deposited or otherwise formed) in the volume of or on a surface of a substrate 110. The grating 112 is a periodic or aperiodic variation in the effective refractive index and/or effective optical absorption of at least a portion of the substrate 110.

The substrate 110 has an inner region 120 where the grating 112 is located. The inner region may be photosensitive to allow the writing or impressing of the grating 112. The substrate 110 has an outer region 118 which does not have the grating 112 therein.

The grating 112 is a combination of one or more individual spatial periodic sinusoidal variations in the refractive index that are collocated along the length of the grating region 120 of the substrate 110, each having a spatial period (or pitch) Λ. The grating 112 (or a combination of gratings) represents a unique optically readable code, made up of bits. In one embodiment, a bit corresponds to a unique pitch Λ within the grating 112.

The grating 112 may also be referred to herein as a composite or collocated grating. Also, the grating 112 may be referred to as a “hologram”, as the grating 112 transforms, translates, or filters an input optical signal to a predetermined desired optical output pattern or signal.

The substrate 110 comprises silica glass (SiO₂) having the appropriate chemical composition to allow the grating 112 to be disposed therein or thereon. Other materials for the optical substrate 110 may be used if desired. For example, the substrate 110 may be made of any glass, e.g., silica, phosphate glass, borosilicate glass or other glasses, or made of glass and plastic, or solely plastic. For high temperature or harsh chemical applications, the optical substrate 110 made of a glass material is desirable. If a flexible substrate is needed, a plastic, rubber or polymer-based substrate may be used. The optical substrate 110 may be any material capable of having the grating 112 disposed in the grating region 120 and that allows light to pass through it to allow the code to be optically read.

The optical substrate 110 with the grating 112 has a length. L and an outer diameter D1, and the inner region 120 diameter D. The length L can range from very small (about 1-1000 microns or smaller) to large (about 1.0-1000 mm or greater). In addition, the outer dimension D1 can range from small (less than 1000 microns) to large (1.0-1000 mm and greater). Other dimensions and lengths for the substrate 110 and the gating 112 may be used.

The grating 112 may have a length Lg of about the length L of the substrate 110. Alternatively, the length Lg of the grating 112 may be shorter than the total length L of the substrate 110.

Moreover, referring to FIG. 6, the size of any given dimension for the region 120 of the grating 112 may be less than any corresponding dimension of the substrate 110. For example, if the grating 112 has dimensions of length Lg, depth Dg, and width Wg, and the substrate 110 has dimensions of length L, depth D, and width W, the dimensions of the grating 112 may be less than that of the substrate 110. Thus, the grating 112, may be embedded within or part of a much larger substrate 110. Instead of rectangular dimensions or coordinates for size of the substrate 110, the element 108, or the grating 112, other dimensions/coordinates for size may be used, e.g., polar or vector dimensions.

Also, the element 108 may be embedded or formed in or on a larger object for identification of the object.

The substrate 110 may have end-view cross-sectional shapes other than circular, such as square, rectangular, elliptical, clam-shell, D-shaped, or other shapes, and may have side-view sectional shapes other than rectangular, such as circular, square, elliptical, clam-shell, D-shaped, or other shapes. Also, 3D geometries other than a cylinder may be used, such as a sphere, a cube, a pyramid, a bar, a slab, a plate, a brick, or a disc shape, or any other 3D shape. Alternatively, the substrate 110 may have a geometry that is a combination of one or more of the foregoing shapes.

The dimensions, geometries, materials, and material properties of the substrate 110 are selected such that the desired optical and material properties are met for a given application. The resolution and range for the optical codes are scalable by controlling these parameters (discussed more hereinafter).

The substrate 110 may be coated with a polymer material or other material that may be dissimilar to the material of the substrate 110, provided that the coating on at least a portion of the substrate, allows sufficient light to pass transversely through the substrate for adequate optical detection of the code using side illumination.

Referring to FIG. 7, illustrations (a)-(c), for the grating 112 in a cylindrical substrate 110 having a sample spectral 17 bit code (i.e., 17 different pitches Λ1-Λ17), the corresponding image on a CCD (Charge Coupled Device) camera is shown for a digital pattern of 17 bit locations 89. FIG. 7(b), (c), and (a), respectively, illustrate 7 bits turned on (10110010001001001); 9 bits turned on (11000101010100111); and all 17 bits turned on (11111111111111111).

For the images in FIG. 7, the length of the substrate 110 was 450 microns, the outer diameter D1 was 65 microns, the inner diameter D was 14 microns, δn for the grating 112 was about 10-4, n1 in portion 120 was about 1.458 (at a wavelength of about 1550 nm), n2 in portion 118 was about 1.453, the average pitch spacing Λ for the grating 112 was about 0.542 microns, and the spacing between pitches ΔΛ was about 0.36% of the adjacent pitches Λ.

The grating 112 may be impressed in the substrate 110 by any technique for writing, impressed, embedded, imprinted, or otherwise forming a diffraction grating in the volume of or on a surface of a substrate 110. Examples of some known techniques are described in U.S. Pat. Nos. 4,725,110 and 4,807,950, entitled “Method for Impressing Gratings Within Fiber Optics”, to Glenn et al; and U.S. Pat. No. 5,388,173, entitled “Method and Apparatus for Forming A periodic Gratings in Optical Fibers”, to Glenn, respectively, and U.S. Pat. No. 5,367,588, entitled “Method of Fabricating Bragg Gratings Using a Silica Glass Phase Grating Mask and Mask Used by Same”, to Hill, and U.S. Pat. No. 3,916,182, entitled “Periodic Dielectric Waveguide Filter”, Dabby et al, and U.S. Pat. No. 3,891,302, entitled “Method of Filtering Modes in Optical Waveguides”, to Dabby et al, which are all incorporated herein by reference to the extent necessary to understand the present invention.

Alternatively, instead of the grating 112 being impressed within the substrate material, the grating 112 may be partially or totally created by etching or otherwise altering the outer surface geometry of the substrate to create a corrugated or varying surface geometry of the substrate, such as is described in U.S. Pat. No. 3,891,302, entitled “Method of Filtering Modes in Optical Waveguides”, to Dabby et al, which is incorporated herein by reference to the extent necessary to understand the present invention, provided the resultant optical refractive profile for the desired code is created.

Further, alternatively, the grating 112 may be made by depositing dielectric layers onto the substrate, similar to the way a known thin film filter is created, so as to create the desired resultant optical refractive profile for the desired code.

Unless otherwise specifically stated herein, the term “microbead” is used herein as a label and does not restrict any embodiment or application of the present invention to certain dimensions, materials and/or geometries.

Applications, Uses, Geometries and Embodiments for the Encoded Element of the Present Invention

Applications, uses, geometries and embodiments for the encoded element of the present invention may be the' same as that described in the following cases which are all incorporated herein by reference in their entirety: U.S. patent application Ser. No. 10/661,234 (CyVera Docket No. CV-0038A), filed Sep. 12, 2003, entitled “Diffraction Grating-Based Optical Identification Element”; U.S. patent application Ser. No. 10/661,031 (CyVera. Docket No. CV-0039A) filed Sep. 12, 2003, entitled “Diffraction Grating-Based Encoded Micro-particles for Multiplexed Experiments”; U.S. patent application Ser. No. 10/661,082 (CyVera Docket No. CV-0040), filed Sep. 12, 2003, entitled “Method and Apparatus for Labeling Using Diffraction Grating-Based Encoded Optical Identification Elements”; U.S. patent application Ser. No. 10/661,115 (CyVera Docket No. CC-0041), filed Sep. 12, 2003, entitled “Assay Stick”; U.S. patent application Ser. No. 10/661,836 (CyVera Docket No. CV-0042), filed Sep. 12, 2003, entitled “Method and Apparatus for Aligning Microbeads in order to Interrogate the Same”; U.S. patent application. Ser. No. 10/661,254 (CyVera Docket No. CV-0043), filed Sep. 12, 2003, entitled “Chemical Synthesis Using Diffraction Grating-based Encoded Optical Elements”; U.S. patent application Ser. No. 10/661,116 (CyVera Docket No. CV-0044), filed Sep. 12, 2003, entitled “Method of Manufacturing of a Diffraction grating-based identification Element”; and U.S. patent application Ser. No. 10/763,995 (CyVera Docket No. CV-0054), filed Jan. 22, 2004, entitled, “Hybrid Random Bead/Chip Based Microarray”, U.S. Provisional Patent Application Ser. Nos. 60/609,583, 60/610,059 and 60/609,712, all filed Sep. 13, 2004 (CV-0082PR, 83PR and 84PR); U.S. Provisional Patent Application Ser. Nos. 60/611,205, 60/610,910, 60/610,833, 60/610,829, 60/610,928, all filed Sep. 17, 2004 (CV-0085PR, 86PR, 87PR, 88PR and 89PR); U.S. Provisional Patent Application Ser. No. 60/611,676, filed Sep. 20, 2004 (CV-OO91PR); and U.S. patent application Ser. No. 10/956,791, filed Oct. 1, 2004 (CV-0092 US).

Computer Programs and Other Data Processing Methods

Various aspects of the present invention may be conducted in an automated or semi-automated manner, generally with the assistance of well-known data processing methods. Computer programs and other data processing methods well known in the art may be used to store information including e.g. microbead identifiers, probe sequence information, sample information, and binding signal intensities. Data processing methods well known in the art may be used to read input data covering the desired characteristics.

APPLICATIONS

The invention may be used in many areas such as drug discovery, functionalized substrates, biology, proteomics, combinatorial chemistry, DNA analysis/tracking/sorting/tagging, as well as tagging of molecules, biological particles, matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, radioactive or non-radioactive proximity assays, and other assays, (including fluorescent, mass spectroscopy), high throughput drug/genorne screening, and/or massively parallel assay applications. The invention provides uniquely identifiable beads with reaction supports by active coatings for reaction tracking to perform multiplexed experiments.

SCOPE OF THE INVENTION

The dimensions and/or geometries for any of the embodiments described herein are merely for illustrative purposes and, as such, any other dimensions and/or geometries may be used if desired, depending on the application, size, performance, manufacturing requirements, or other factors, in view of the teachings herein.

It should be understood that, unless stated, otherwise herein, any of the features, characteristics, alternatives or modifications described regarding a particular embodiment herein may also be applied, used, or incorporated with any other embodiment described herein. Also, the drawings herein are not drawn to scale.

Although the invention has been described and illustrated with respect to exemplary embodiments thereof, the foregoing and various other additions and omissions may be made therein and thereto without departing from the spirit and scope of the present invention.

Moreover, the invention comprises the features of construction, combination of elements, and arrangement of parts which will be exemplified in the construction hereinafter set forth.

It will thus be seen that the objects set forth above, and those made apparent from the preceding description, are efficiently attained and, since certain changes may be made in the above construction without departing from the scope of the invention, it is intended that all matter contained in the above description or shown in the accompanying drawing shall be interpreted as illustrative and not in a limiting sense.

Claims

1-63. (canceled)

64. An encoded microparticle comprising: an optical substrate comprising a material that permits light to propagate therethrough, the optical substrate having an elongated body that extends in a direction along a central axis, the optical substrate including an outer region that extends about the central axis; andan optically detectable code disposed within the optical substrate and extending along the central axis, the outer region surrounding the optically detectable code about the central axis, the optically detectable code being readable when the light propagates through the outer region and is at least one of reflected or filtered by the optically detectable code, said at least one of reflected or filtered light propagating through the outer region to be detected for reading the optically detectable code.

65. The encoded microparticle in accordance with claim 64, wherein the optical substrate includes etched outer surfaces.

66. The encoded microparticle in accordance with claim 64, wherein the optical substrate is shaped from a photolithographic process.

67. The encoded microparticle in accordance with claim 64, wherein the optical substrate has planar sides that extend parallel to the central axis.

68. The encoded microparticle in accordance with claim 67, wherein the planar sides are etched planar sides.

69. The encoded microparticle in accordance with claim 67, wherein the planar sides are shaped from a photolithographic process.

70. The encoded microparticle in accordance with claim 67, wherein the optical substrate is rectangular along the central axis and has a square cross-section.

71. The encoded microparticle in accordance with claim 67, wherein the planar sides include four planar sides that extend parallel to the central axis.

72. The encoded microparticle in accordance with claim 67, wherein said at least one of reflected or filtered light is permitted to propagate through any one of the planar sides.

73. The encoded microparticle in accordance with claim 64, wherein a length of the optically detectable code is less than a length of the optical substrate.

74. The encoded microparticle in accordance with claim 64, wherein the outer region completely surrounds the optically detectable code in a uniform manner about the central axis.

75. The encoded microparticle in accordance with claim 64, wherein the optical substrate comprises silicon dioxide (SiO2).

76. The encoded microparticle in accordance with claim 64, wherein the optically detectable code comprises a photosensitive material.

77. The encoded microparticle in accordance with claim 64, wherein the optically detectable code is defined by a grating having a periodic or aperiodic variation in at least one of a refractive index and an optical absorption.

78. The encoded microparticle in accordance with claim 64, wherein the optically detectable code is one of written, impressed, embedded, imprinted, etched, grown, and deposited within the optical substrate.

79. The encoded microparticle in accordance with claim 64, wherein the optically detectable code is defined by a diffraction grating.

80. The encoded microparticle in accordance with claim 64, wherein the optically detectable code is formed from dielectric layers.

81. The encoded microparticle in accordance with claim 64, wherein the optical substrate provides a reaction support to be used during a multiplexed experiment.

82. The encoded microparticle in accordance with claim 81 further comprising nucleic acids attached to the optical substrate.

83. The encoded microparticle in accordance with claim 64, wherein the encoded microparticle comprises a plurality of encoded microparticles in which differently encoded microparticles have optically detectable codes that are unique with respect to each other.