Claims
- 1. A method to monitor progression, angiogenesis and/or metastasis of tumor cells or tissue on the internal organs of a tumor model system, which system is a immunocompromised or syngeneic experimental animal, which method comprises
observing fluorescent tumor cells and/or tissue in said model system through an opened skin flap; wherein said skin flap is arranged to be reversibly opened and closed.
- 2. A method to evaluate the effect of a compound or protocol on cancer progression, angiogenesis and/or metastasis, which method comprises
performing the method of claim 1 in the presence and absence of the compound or protocol to be tested, and comparing the results obtained, whereby the effect of said compound or protocol on cancer progression, angiogenesis, and/or metastasis is evaluated.
- 3. The method claim 1 or 2 wherein the immunocompromised experimental animal is a mouse or rat.
- 4. The method of claim 1 or 2 wherein said fluorescent tumor cells have been modified to contain a nucleic acid molecule for expression of a fluorescent protein.
- 5. The method of claim 4 wherein the fluorescent protein emits green fluorescence.
- 6. The method of claim 4 wherein the fluorescent protein emits red fluorescence.
- 7. The method of claim 1 or 2 wherein the experimental animal is provided tumor cells by subcutaneous injection.
- 8. The method of claim 1 or 2 wherein the experimental animal is provided tumor tissue by surgical orthological implantation (SOI).
- 9. The method of claim 1 or 2 wherein the tumor tissue is endogenous and is made fluorescent by infecting with a viral vector for expression of fluorescent proteins.
- 10. The method of claim 1 or 2 wherein the skin flap is an arc-shaped flap.
- 11. The method of claim 1 or 2 wherein the tumor cells are tissues or of brain, lung, liver, colon, breast, prostate, ovary or pancreas.
- 12. The method of claim 1 or 2 wherein the observing is macroscopic.
- 13. The method of claim 1 or 2 wherein the observing is microscopic.
- 14. The method of claim 1 or 2 wherein said tumor cells or tissue comprise nucleic acid molecules for production of fluorescent proteins of at least two different colors.
- 15. A method to observe simultaneously, the progression, angiogenesis and/or metastasis of at least first and second tumor cells or tissues, which method comprises
the step of providing at least a first tumor cell or tissue which is modified to express fluorescent protein that emits a first color and a second cell or tissue which is modified to express a fluorescent protein which emits a second color; and observing said cells or tissues in an immunocompromised or syngeneic experimental animal.
- 16. The method of claim 15 wherein said first tumor cells are tissues are of different origin from said second cells or tissues.
- 17. The method of claim 15 wherein said cells are tissues are of prostate, breast, or fibrosarcoma.
Parent Case Info
[0001] This application claims priority under 35 U.S.C. §119(e) to U.S. provisional application Serial No. 60/322,291 filed Sep. 10, 2001. The contents of which are incorporated herein by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60322291 |
Sep 2001 |
US |