Claims
- 1. Protein fusions having a formula selected from the group consisting of E1-Ln-E2 or E2-Ln-E1, wherein E1 and E2 are selected from the group comprising β-ketothiolases, acyl-CoA reductases, PHA synthases, PHB synthetases, phasins, enoyl-CoA hydratases and beta-hydroxyacyl-ACP::coenzyme-A transferase, in which Ln is a peptide of n amino acids that links E1 to E2 or E2 to E1.
- 2. The fusion of claim 1 selected from the group consisting of beta-ketothiolase (phbA) and acyl-CoA reductase (phbB); phbB and phbA; PHA synthase (phaC) and phasin (phaP); phaP and phaC (1D); phaC and beta-hydroxyacyl-ACP::coenzyme-A transferase (phbG); phbG and phaC; phaC and enoyl-CoA hydratases (phaJ); and phaJ and phaC.
- 3. The fusion of claim 1 wherein n in the linker is between zero and 50 amino acids.
- 4. The fusion of claim 1 wherein the linker is glycine-serine.
- 5. The fusion of claim 1 expressed in a plant.
- 6. The fusion of claim 1 expressed in a bacteria.
- 7. A gene encoding protein fusions having a formula selected from the group consisting of E1-Ln-E2 or E2-Ln-E1, wherein E1 and E2 are selected from the group comprising β-ketothiolases, acyl-CoA reductases, PHA synthases, PHB synthetases, phasins, enoyl-CoA hydratases and beta-hydroxyacyl-ACP::coenzyme-A transferase, in which Ln is a peptide of n amino acids that links E1 to E2 or E2 to E1.
- 8. The gene of claim 7 encoding a fusion protein selected from the group consisting of beta-ketothiolase (phbA) and acyl-CoA reductase (phbB); phbB and phbA; PHA synthase (phaC) and phasin (phaP); phaP and phaC (1D); phaC and beta-hydroxyacyl-ACP::coenzyme-A transferase (phbG); phbG and phaC; phaC and enoyl-CoA hydratases (phaJ); and phaJ and phaC.
- 9. The gene of claim 7 wherein n in the linker is between zero and 50 amino acids.
- 10. The gene of claim 7 wherein the linker is glycine-serine.
- 11. The gene of claim 7 comprising a promoter for expression in plants.
- 12. The gene of claim 11 comprising a promoter specific for expression in a tissue, plastid or other organ.
- 13. The gene of claim 11 comprising a promoter specific for expression during a regulatory phase.
- 14. The gene of claim 7 further comprising RNA processing signals or ribozyme sequences.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] Priority is claimed to U.S. provisional application Serial No. 60/094,674, filed Jul. 30, 1998.
Provisional Applications (1)
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Number |
Date |
Country |
|
60094674 |
Jul 1998 |
US |