Estrogen receptor beta variants and methods of detection thereof

Abstract

The present invention is based on sequencing genomic DNA from human chromosome 6 and cDNAs to define the genomic structure of estrogen receptor beta genes and novel polymorphism in the estrogen receptor gene/protein. Such polymorphism can lead to a variety of disorders that are mediated/modulated by a variant estrogen receptor, such as a susceptibility to cancer, osteoporosis, cardiovascular disorder, etc. Based on this sequencing approach, the present invention provides genomic nucleotide sequences, cDNA sequences, amino acid sequences and sequence polymorphism in the ESR-beta genes, methods of detecting these sequences/polymorphism in a sample, methods of determining a risk of having or developing a disorder mediated by a variant estrogen receptor and methods of screening for compounds used to treat disorders mediated by a variant estrogen receptor.

Description

FIELD OF THE INVENTION

The present invention is in the field of disease detection and therapy. The present invention specifically provides the identification of previously unknown nucleic acid/amino acid polymorphisms within the estrogen receptor beta gene (ESR-beta) and the genomic sequence of this gene for use in the development of diagnostics and therapies for diseases and disorders mediated/modulated by the estrogen receptor.

BACKGROUND OFF THE INVENTION

Estrogen Receptor

The human estrogen receptor beta belongs to the nuclear hormone receptor family. Nuclear hormone receptors are a family of hormone-activated transcription factors that can initiate or enhance the transcription of genes containing specific hormone response elements.

The ER protein consists of 595 amino acids with a molecular weight of 66 kDa, 8 transcribed exons, with six different functional domains. Two of those domains are highly conserved in the primary sequence of members of the nuclear hormone receptor superfamily. One of the domains, the DNA binding domain (DBD), contains two zinc fingers that mediate receptor binding to hormone response elements in the promoters of hormone-responsive genes. In the C-terminal region, the hormone-binding domain (HBD) contains two regions of sequence homology with other hormone receptors and gives hormone specificity and selectivity. The human ER-alpha gene is located in chromosome 6q.25.1.Estrogen receptors, like other steroid receptors, are transcription factors that are activated upon binding to steroids (estradiol) or steroid analogs such as tamoxifen. Upon activation the receptors dimerize to form homodimers or heterodimers that bind to estrogen receptor elements (EREs) located in the promoter region of estrogen-activated genes and coordinate transcription by interacting with host co-activators.

Role of Estrogen in Cardiovascular Disease

Heart disease is the leading cause of mortality in women, a fact that is under appreciated by both women and physicians. One in 9 women aged 45-65 have some form of cardiovascular disease and the number increases to 1 in 3 after age 65. Each year, 240,000 U.S. women die from heart disease, and nearly 90,000 die of stroke. Moreover, approximately 44% die within one year of suffering a heart attack, compared with 26% of men (Warren M P and Kulak J Clin Obs Gyn 1998 41(4):976-987).

Estrogens exert a wide range of physiological effects on a large variety of cell types. For example, they regulate cell growth and apoptosis and a myriad of functions related to reproduction. There are two types of estrogen receptors, beta and beta. Blood vessels and bone contain beta receptors, the liver has beta receptors, and both beta and beta receptors are found in the central nervous system. The interaction of these different receptor sites influences the biological effects of estrogen and selective estrogen receptor modulators (SERMs), such as raloxifene. The binding patterns dictate whether an estrogen or a SERM acts as an estrogen agonist or an antagonist (Mendelsohn ME and Karas R H New Engl J Med 1999, 340(23):1801-1811; Grese T A and Dodge J A Curr Pharm Design 1998, 4:71-92). Tissue-specific relationships exist between SERMs and the receptor binding sites. Estrogens also increase high-density lipoprotein cholesterol levels, decrease low-density lipoprotein cholesterol, and decrease plasminogen-activating inhibitor levels (Meisler J G Jour Women's Health 1999, 8(1):51-57). All estrogens require cellular receptors for their expression. In general, estrogen receptors are ligand-inducible transcription factors, which regulate the expression of target genes after hormone binding (Faustini-Fustini et al. Eur J Endocrin 1999, 140:111-129). Estrogen may also have important effects on the vascular wall. Estradiol and progesterone receptors have been identified in arterial endothelial and smooth muscle cells (Campisi D et al. Int J Tiss React 1987, IX(5):393-398). Estrogens act on the wall of the artery to relax vascular smooth muscle and to decrease vascular resistance. The mechanism appears to be through stimulation of endothelial-derived relaxing factors and an endogenous nitrate (Warren M P and Kulak J Clin Obs Gyn 1998 41(4):976-987). The relaxation induced by 17B-estradiol may play an important role in the regulation of coronary tone, which reduces the risk of coronary disease in postmenopausal women. The production of nitric oxide is mediated by the estrogen receptor, because when the receptor is blocked by an antiestrogen agent, nitric oxide is suppressed.

Several studies have shown that estrogen therapy reduces the risk of heart disease by up to 50% (most recently reviewed by Mendelsohn M E and Karas R H New Engl J Med 1999, 340(23):1801-1811; Rich-Edwards J W N Engl J Med, 1995, 332:1758-1765; Gerhard M, Ganz P, Circulation, 1995, 92:5-8; Grodstein F, et al N Engl J Med 1997, 336:1769-75; Chasen-Taber L and Stampfer M J Ann of Int Med, 1998, 128:467-477; Warren M P and Kulak J Clin Obstet Gyn 1998, 41(4):976-987). Loss of estrogen may be one of the most important factors in the development of cardiovascular disease in women.

While there is no direct evidence that estrogen prevents atherogenesis, considerable epidemiologic evidence exists that suggests that estrogens may have some benefit in reducing cardiovascular disease: (1) In all age groups, women have a lower incidence of cardiovascular disease than do men; (2) women who undergo a premature surgical menopause and do not take estrogens are twice as likely to have cardiovascular disease are age-matched premenopausal controls; (3) postmenopausal women who use estrogens have a significantly lower incidence of cardiovascular disease compared with those who do not; and (4) women with coronary artery disease detected by angiography have a higher survival rate if they are estrogen users.

In recent years, reports of favorable effects of estrogen therapy on cardiovascular morbidity and mortality have led to enthusiasm for widespread use of estrogens by postmenopausal women (Meinertz T Herz 1997, 22: 151-157). Guidelines for estrogen therapy issued by the American College of Physicians include the statement “Women who have coronary heart disease are likely to benefit from hormone therapy.”

More than 30 prospective studies and 13 case controlled studies have examined the effect of estrogen replacement therapy on cardiovascular incidence or prevalence and all cause mortality (Stampfer M J et al. New Engl J Med 1991, 325:756-62; Grady D et al. Ann Intern Med 1992, 117:1016-37). The majority of these studies showed lower morbidity and mortality from coronary heart disease among users of postmenopausal estrogens than among non-users. Specifically, they have shown that coronary artery disease in estrogen takers is approximately 50% that in women who do not take estrogen. Overall, the bulk of the evidence strongly supports a protective effect of estrogens yielding a relative risk of 0.56 (95% confidence interval 0.50-0.61). However, a “healthy woman selection bias” is present in these studies and potentially may confound these results (estrogen takers have better weight control, exercise more, and smoke less than women who are not prescribed estrogen). Moreover, other biases such as estrogen takers tend to have higher education, higher income, etc., are confounding these epidemiologic studies (Abrams J Clin Cardiol 1998, 21:218-222).

Since the earlier observational trials were not randomized, it is believed by many that as much as 25% of this 50% reduction in risk is due to these various methodological biases (Barrett-Conner E and Grady D 1998, Ann Rev Public Health 19:55-72). Recently, 2 meta-analyses estimated the reduction in coronary heart disease associated with estrogen use to be in the range of 35 to 44%, respectively (Grodstein F and Stampfer M J Prog Cardiol Dis 1995, 38: 199-210; Barrett-Conner E and Grady D 1998, Annu Rev Public Health 19:55-72). Recent studies are exploring the issue of opposed vs unopposed estrogen, because of a documented increased risk for uterine cancer in women with an intact uterus who are taking estrogen alone. The new lines of evidence are suggesting that women taking estrogen plus a progestin (usually a medroxyprogesterone acetate) do not receive an equivalent benefit from the cardioprotective effects compared to women taking estrogen alone (Hulley S et al 1998 JAMA 280:605-613; Abrams J Clin Cardiol 1998, 21:218-222).

The loss of estrogen at menopause is associated with a 6% decline in HDL cholesterol levels and a 5% rise in LDL cholesterol levels, which may explain the higher cardiovascular disease rate among postmenopausal women compared with premenopausal women. The lower incidence of cardiovascular disease among postmenopausal women who take estrogen may be explained in part by the resultant 15% to 19% decrease in LDL cholesterol levels and the 16% to 18% increase in HDL cholesterol levels (JAMA 1995, 273:199-208). The PEPI (Postmenopausal Estrogen/Progestin Intervention, a randomized, double-blind placebo-controlled trial, showed that HDL cholesterol levels rose significantly more in women assigned to estrogen alone than in women assigned the combined estrogen (JAMA 1995, 273:199-208). Recent non-human primates studies substantiate these findings (Clarkson T B Lab An Sci 1998, 48(6):569-72). Statistical modeling of the effect of estrogen on lipid profiles indicates that 25-50% of the apparent cardioprotection due to estrogen is mediated by favorable changes in HDL-cholesterol (Bush T L et al. 1987 Circulation 75:1102-9; Gruchow H W et al. 1988 Am Heart J 115:954-63).

Estrogen replacement therapy is not without risk. For years, studies have shown a 3-4-fold increased risk of venous thromboembolism (VTE) in users of oral contraceptives compared to non-users (Weiss G Am J Obstet Gynecol 1999 180:S295-301). One study has shown that intrinsic coagulation factors play a significant role in oral contraceptive-associated VTE (Vandenbroucke J P et al. Lancet 1994 344:1453-7; Rosing J et al. Br J Haematol 1997, 97:233-238). The Factor V Leiden mutation increases risk of VTE 5-10 fold in non users, but 30-fold in third-generation oral contraceptive users. Combined estrogens appear to induce resistance to the body's natural anticoagulation system (APC). Heterozygotes for the Factor V Leiden mutation who take oral contraceptives develop APC resistance as high as that seen in women who are homozygous.

Estrogens increase the risk of endometrial carcinoma approximately 6-fold, an effect that is eliminated, for the most part, by the addition of progestins (Barrett-Conner E and Grady D 1998, Ann Rev Public Health 19:55-72). Controversy continues over whether estrogen replacement increases the risk of breast cancer, but some studies indicate risk is elevated by as much as 30%. (Greendale GA et al. Lancet 1999, 353:571-80).

A number of prospective randomized studies designed to definitely establish whether estrogen replacement therapy reduces the risk of cardiovascular disease in women and whether it increases the risk of breast cancer, are underway. One recently completed trial (HERS—Heart and Estrogen/progestin Replacement Study) compared continuous combined estrogen plus medroxyprogesterone acetate to placebo in 2700 women with pre-existing coronary disease (Hully S et al. 1998 JAMA 280(7):605-13). Compared to controls, the intervention group had significantly more heart disease events in year one of the trial, but significantly fewer events in years 4 and 5 of the trial. Moreover, a significant increase in the rate of thromboembolic events occurred in the early years of the study in women taking hormones. Based on these results, hormone replacement therapy is not recommended for secondary prevention of heart disease.

Two other large, ongoing clinical trials on primary prevention of cardiovascular disease using estrogens are underway. The Women's Health Initiative, due to be completed in 2005 and a U.K study called WIS-DOM, due to be completed in 2010, should shed new light on the protective effects of estrogen on cardiovascular disease (Meisler J G Jour Women's Health 1999, 8(1):51-5).

In summary, ongoing research suggests that estrogen replacement therapy, particularly involving recently formulated designer estrogens or SERMs, may have beneficial effects on the cardiovascular system as well as bone, without the untoward effects on breast and endometrial tissue. Caution still needs to be observed, nonetheless. Women who take estrogens are, on average, better educated, healthier, have higher incomes and have better access to health care. These differences rather than the estrogens may account for much of the lower risk of heart disease.

For postmenopausal women without frank disease, estrogen replacement therapy appears to have a beneficial effect when one considers the magnitude, consistency, and biological plausibility of the data. For women with pre-existing disease, questions remain as to the safety and efficacy of exogenous estrogens as protective agents against cardiovascular disease.

Estrogen and Autoimmune Diseases

A. Systemic Lupus Erythematosus

There is a widely held view that estrogens play a role in Systemic lupus erythematosus because:

1. Women of child bearing age are nine times more likely to develop systemic lupus erythematosus than men. Prior to pubescence the rate is three fold higher in females, while post menopausal women have an equal chance of developing SLE as aged matched males. Many studies have been done that show that the reason for the differences in the sexes is probably estrogen related (Lahita R. G., 1986: Springer Seminars in Immunopathology 9, 305-314; Krammer, G. M. and Tsokos, G. C., 1998 Clinical Immunology and Immunopathology 89: 192-195; Rider at al., 1998 Clinical Immunology and Immunopathology 89: 171-180).

Clues to the role of estrogens in SLE came from studies that concluded that oral contraceptives adversely affected the morbidity of this illness (Buton, J. P., 1996 Ann. Med. Interne, 147:259-264; Julkunen, 1991: Scan. J. Rheumatol. 20:427-433).

2. Patients with Klinefelter syndrome (XXY), have been reported with SLE (Stern et al., 1977: Arthritis and Rheumatism 20:18-22).

3. Patients with SLE have anti-estrogen antibodies (Feldman, 1987: Biochem. Biophys. Acta, 145:1342-1348: Bucala et al., 1987: Clin. Exp. Immunol. 67:167-175)

In the past, oral contraceptives have been shown to cause flare ups of SLE, their use was discouraged in women with SLE, while the current thinking is that the lower dose birth control pills are safe for SLE patients (Julkunen H A Scand J Rheumatol 1991;20(6):427-33). As well hormone replacement therapy is considered safe for SLE patients (Mok et al., Scand J Rheumatol 1998;27(5):342-6: Kreidstein et al., 1997 , J Rheumatol 1997 November;24(11):2149-52)

4. The estrogen antagonist tamoxofin seems to improve the course of the disease (Sthoeger, 1997 , Ann NY Acad Sci 1997 Apr. 5;815:367-8: Sthoeger, 1994 , J Rheumatol 1994 December;21(12):2231-8).

B. Estrogen, Rheumatoid Arthritis (RA) and Osteoarthritis

The literature surrounding the involvement of estrogens in Rheumatoid arthritis is less clear than with osteoarthritis. Epidemiological studies suggests that RA is influenced by female sex hormones, by one study states that the use of oral contraceptives may postpone the onset of RA, but that estrogens alone no not alleviate the symptoms of RA (Bijlsma Am J Reprod Immunol 1992 October-December;28(3-4):231-4). Adjuvant oestrogen treatment does increase bone mineral density in postmenopausal women with RA, and may protect against osteophoresis which is often a complication of RA (van den Brink: Ann Rheum Dis 1993 April;52(4):302-5). While the study mentioned above indicated that estrogens did not alleviate RA symptoms, another study concluded that adjuvant estrogen therapy did not even improve the symptoms. One polymorphism has been reported in the estrogen receptor that seems to be associated with the age of onset of RA (Ushiyama Ann Rheum Dis 1999 January;58(1):7-10)

Osteoarthritis on the other hand is less prevalent in postmenopausal women who take estrogen replacement therapy (ERT) (Felson Curr Opin Rheumatol 1998 May;10(3):269-72) suggesting that ERT may be beneficial in preventing osteoarthritis.

C. Estrogen and Osteophorosis

Osteophorosis is a metabolic bone disorder that leads to bone fragility and subsequent risk of fracture. Treatment for postmenopausal women with osteophoresis includes hormone replacement, in particular estrogen. Estrogen has shown to reduce the incidence of bone loss and fractures (Weiss et al., N Engl J Med 1980 Nov. 20;303(21):1195-8:Paganini-Hill et al., Ann Intern Med 1981 July;95(1):28-31: Ettinger et al., Ann Intern Med 1985 March;102(3):319-24)

Further, polymorphisms in the estrogen receptor have been associated with bone loss in both humans and mice. (Kobayashi J Bone Miner Res 1996 March;11(3):306-11: Kurabayashi Am J Obstet Gynecol 1999 May; 180(5):1115-20; Deng Hum Genet 1998 November;103(5):576-85)

Estrogens and Cognitive Function

Compared with men, women are at greater risk of developing Alzheimer's disease. Several studies show that women who take estrogen after menopause have a lower incidence of Alzheimer's disease. Among women with Alzheimer's, those taking estrogen suffer less severe symptoms and slower mental deterioration. The duration of estrogen use also seems to be important in reducing risk. Women with a history of long-term term use (more than 10 years) had the lowest risk. But even women who took estrogen for a short time also benefited.

Estrogen and Breast Cancer

The major risk factors for the development of breast cancer are sex, age, family history of breast cancer, age of menarche, age at first full-term pregnancy, and age of menopause. All of these factors, with the exception of family history, have been shown to be directly associated with lifetime exposure to estrogen, increased hormone exposure being associated with increased risk of developing breast cancer. The increased cancer risk is believed to be caused by an estrogen receptor-mediated proliferative response in cells of the mammary epithelium.

Tamoxifen, an estrogen receptor antagonist, has been shown to be an effective agent for both the prevention and treatment of breast cancer. Using immunohistochemical methods, it is possible to classify breast tumors as being estrogen receptor positive or negative, depending upon the amount of estrogen receptor protein expressed in the tissue. Estrogen receptor positive tumors are more likely to respond to treatment with tamoxifen than estrogen receptor negative tumors. Pre-menopausal women are more likely to develop estrogen receptor negative breast cancers than are post-menopausal women.

Mutations altering the structure and function of the estrogen receptor have been described in primary breast tumors or breast cancer cell lines. It is not clear however whether these changes are primary (and involved in the processes leading to carcinogenesis) or secondary (and a consequence of genetic instability in cancer tissues). In addition to these somatic mutations, some studies have pointed to a possible association between inherited DNA sequence changes and the development of breast cancer, but these studies are also controversial.

Further evidence for the role of estrogen receptors in breast cancer comes from the recent finding that the gene BRCA 1, which when inherited in a mutant form predisposes to the development of breast cancer, inhibits estrogen receptor signaling.

Estrogens and Endometrial Cancer

Carcinoma of the endometrium is the most common pelvic malignancy in women, however because in approximately 75% of cases it is confined to the body of the uterus at the time of diagnosis, it can usually be cured by hysterectomy. Unopposed exposure of endometrial cells to estrogens dramatically increases the chance of developing this form of uterine cancer and it is for this reason that hormone replacement therapy consisting solely of estrogen should not be given to women with intact uteri. Cyclical or continuous co-administration of progesterone serves to prevent excessive proliferation of endometrial cells, reducing the risk of endometrial cancer in post-menopausal women receiving estrogen as part of hormone replacement therapy regimens.

The majority of cases of endometrial cancers express estrogen receptor and, in general, estrogen responsive tumors have a favorable prognosis. Acquired (somatic) mutations have been described in up to 8.5% of cases, however the role of these mutations in the development and progression of endometrial cancer is uncertain at present.

Although it remains somewhat controversial, studies suggest that use of tamoxifen may increase the chance of developing endometrial cancer. This may be because, in addition to its role in estrogen receptor blockade, tamoxifen has partial receptor agonist activity and results in low-grade induction of estrogen responsive genes that induce endometrial proliferation.

Given the involvement of the estrogen receptor in mediating/modulating various disorders, it is critical to identify sequence polymorphisms in the estrogen receptor and to correlate these with disease states, therapeutic effectiveness and the like. The present invention advances the art by providing a variety of previously unidentified polymorphisms in the ESR-beta protein.

SNPs

The genomes of all organisms undergo spontaneous mutation in the course of their continuing evolution, generating variant forms of progenitor sequences (Gusella, Ann. Rev. Biochem. 55, 831-854 (1986)). The variant form may confer an evolutionary advantage or disadvantage relative to a progenitor form or may be neutral. In some instances, a variant form confers a lethal disadvantage and is not transmitted to subsequent generations of the organism. In other instances, a variant form confers an evolutionary advantage to the species and is eventually incorporated into the DNA of many or most members of the species and effectively becomes the progenitor form. Additionally, the effect of a variant form may be both beneficial and detrimental, depending on the circumstances. For example, a heterozygous sickle cell mutation confers resistance to malaria, but a homozygous sickle cell mutation is usually lethal. In many instances, both progenitor and variant form(s) survive and co-exist in a species population. The coexistence of multiple forms of a sequence gives rise to polymorphisms, such as SNPs.

The reference allelic form is arbitrarily designated and may be, for example, the most abundant form in a population, or the first allelic form to be identified, and other allelic forms are designated as alternative, variant or polymorphic alleles. The allelic form occurring most frequently in a selected population is sometimes referred to as the “wild type” form.

Approximately 90% of all polymorphisms in the human genome are single nucleotide polymorphisms (SNPs). SNPs are single base pair positions in DNA at which different alleles, or alternative nucleotides, exist in some population. The SNP position, or SNP site, is usually preceded by and followed by highly conserved sequences of the allele (e.g., sequences that vary in less than 1/100 or 1/1000 members of the populations). An individual may be homozygous or heterozygous for an allele at each SNP position. As defined by the present invention, the least frequent allele at a SNP position can have any frequency that is less than the frequency of the more frequent allele, including a frequency of less than 1% in a population. A SNP can, in some instances, be referred to as a “cSNP” to denote that the nucleotide sequence containing the SNP is an amino acid coding sequence.

A SNP may arise due to a substitution of one nucleotide for another at the polymorphic site. Substitutions can be transitions or transversions. A transition is the replacement of one purine nucleotide by another purine nucleotide, or one pyrimidine by another pyrimidine. A transversion is the replacement of a purine by a pyrimidine, or vice versa. A SNP may also be a single base insertion/deletion variant (referred to as “indels”). A substitution that changes a codon coding for one amino acid to a codon coding for a different amino acid is referred to as a non-synonymous codon change, or missense mutation. A synonymous codon change, or silent mutation, is one that does not result in a change of amino acid due to the degeneracy of the genetic code. A nonsense mutation is a type of non-synonymous codon change that results in the formation of a stop codon, thereby leading to premature termination of a polypeptide chain and a defective protein.

SNPs, in principle, can be bi-, tri-, or tetra-allelic. However, tri- and tetra-allelic polymorphisms are extremely rare, almost to the point of non-existence (Brookes, Gene 234 (1999) 177-186). For this reason, SNPs are often referred to as “bi-allelic markers”, or “di-allelic markers”.

Causative SNPs are those SNPs that produce alterations in gene expression or in the expression or function of a gene product, and therefore are most predictive of a possible clinical phenotype. One such class includes SNPs falling within regions of genes encoding a polypeptide product, i.e. cSNPs. These SNPs may result in an alteration of the amino acid sequence of the polypeptide product (i.e., non-synonymous codon changes) and give rise to the expression of a defective or other variant protein. Furthermore, in the case of nonsense mutations, a SNP may lead to premature termination of a polypeptide product. Such variant products can result in a pathological condition, e.g., genetic disease. Examples of genes in which a polymorphism within a coding sequence gives rise to genetic disease include sickle cell anemia and cystic fibrosis. Causative SNPs do not necessarily have to occur in coding regions; causative SNPs can occur in any region that can ultimately affect the expression and/or activity of the protein encoded by the nucleic acid. Such gene areas include those involved in transcription, such as SNPs in promoter regions, in gene areas involved in transcript processing, such as SNPs at intron-exon boundaries that may cause defective splicing, or SNPs in mRNA processing signal sequences such as polyadenylation signal regions. For example, a SNP may inhibit splicing of an intron and result in mRNA containing a premature stop codon, leading to a defective protein. Consequently, SNPs in regulatory regions can have substantial phenotypic impact.

Some SNPs that are not causative SNPs nevertheless are in close association with, and therefore segregate with, a disease-causing sequence. In this situation, the presence of the SNP correlates with the presence of, or susceptibility to, the disease. These SNPs are invaluable for diagnostics and disease susceptibility screening.

Clinical trials have shown that patient response to treatment with pharmaceuticals is often heterogeneous. Thus there is a need for improved approaches to pharmaceutical agent design and therapy. SNPs can be used to help identify patients most suited to therapy with particular pharmaceutical agents (this is often termed “pharmacogenomics”). Pharmacogenomics can also be used in pharmaceutical research to assist the drug selection process. (Linder et al. (1997), Clinical Chemistry, 43, 254; Marshall (1997), Nature Biotechnology, 15, 1249; International Patent Application WO 97/40462, Spectra Biomedical; and Schafer et al. (1998), Nature Biotechnology, 16, 3.).

Population Studies

Population Genetics is the study of how Mendel's laws and other genetic principles apply to entire. Such a study is essential to a proper understanding of evolution because, fundamentally, evolution is the result of progressive change in the genetic composition of a population. Population genetics thus seeks to understand and to predict the effects of such genetic phenomena as segregation, recombination, and mutation; at the same time, population genetics must take into account such ecological and evolutionary factors as population size, patterns of mating, geographic distribution of individuals, migration and natural selection.

Ideally, one would wish to know how to describe the types and frequencies of genes in a population, to explain how the population's genetic composition came to be the way it is, and to predict how the population would change as a result of natural selection or as a result of artificial selection.

In order to explain many of those issues it is important to understand the existing relation between loci denominated: Linkage.

Linkage is the coinheritance of two or more nonallelic genes because their loci are in close proximity on the same chromosome, such that after meiosis they remain associated more often than the 50% expected for unlinked genes. During meiosis, there is a physical crossing over, it is clear that during the production of germ cells there is a physical exchange of maternal and paternal genetic contributions between individual chromatids. This exchange necessarily separates genes in chromosomal regions that were contiguous in each parent and, by mixing them with retained linear order, results in “recombinants”. The process of forming recombinants through meiotic crossing-over is an essential feature in the reassortment of genetic traits and is central to understanding the transmission of genes.

Recombination generally occurs between large segments of DNA. This means that contiguous stretches of DNA and genes are likely to be moved together. Conversely, regions of the DNA that are far apart on a given chromosome are likely to become separated during the process of crossing-over.

It is possible to use molecular markers to clarify the recombination events that take place during meiosis. Some markers as (CA)n repeats of different lengths are dispersed throughout human DNA and there is little selective pressure in their lengths are used as position markers and regional identifying characters along chromosomes. Those markers can be used to distinguished paternally derived from maternally derived gene regions.

Other markers are Single Nucleotide Polymorphism (SNP), those are biallelic markers, also used to analyzed the transmission of those markers to offspring.

The pattern of a set of markers along a chromosome is referred to as a “Haplotype”. Therefore sets of alleles on the same small chromosmal segment tend to be transmitted as a block through a pedigree. By analyzing the haplotypes in a series of offspring of parents whose haplotypes are known, it is possible to establish which parental segment of which chromosome was transmitted to which child. When not broken up by recombinations, haplotypes can be treated for mapping purposes as alleles at a single highly polymorphic locus.

The existence of a preferential occurrence of a disease gene in association with specific alleles of linked markers is called “Linkage Disequilibrium” (LD). This sort of disequilibrium generally implies that most of the disease chromosomes carry the same mutation and the markers being tested are quite close to the disease gene. For example, there is considerable linkage disequilibrium across the entire HLA locus. The A3 allele is in LD with the B7 and B14 alleles, and as a result B7 and B14 are also highly associated with hemochromatosis. Thus, HLA typing alone can significantly alter the estimate of risk for hemochromatosis, even if other family members are not available for formal linkage analysis. As a result, using a combination of several markers surrounding the presumptive location of the gene, a haplotype can be determined for affected and unaffected family members.

SNP-Based Association Analysis and Linkage Disequilibrium Mapping

SNPs are useful in association studies for identifying particular SNPs, or other polymorphisms, associated with pathological conditions, such as breast cancer. Association studies may be conducted within the general population and are not limited to studies performed on related individuals in affected families (linkage studies). An association study using SNPs involves determining the frequency of the SNP allele in many patients with the disorder of interest, such as breast cancer, as well as controls of similar age and race. The appropriate selection of patients and controls is critical to the success of SNP association studies. Therefore, a pool of individuals with well-characterized phenotypes is extremely desirable. For example, blood pressure and heart rate can be correlated with SNP patterns in hypertensive individuals in whom these physiological parameters are known in order to find associations between particular SNP genotypes and known phenotypes. Significant associations between particular SNPs or SNP haplotypes and phenotypic characteristics can be determined by standard statistical methods. Association analysis can either be direct or LD based. In direct association analysis, causative SNPs are tested that are candidates for the pathogenic sequence itself

In LD based SNP association analysis, random SNPs are tested over a large genomic region, possibly the entire genome, in order to find a SNP in LD with the true pathogenic sequence or pathogenic SNP. For this approach, high density SNP maps are required in order for random SNPs to be located close enough to an unknown pathogenic locus to be in linkage disequilibrium with that locus in order to detect an association. SNPs tend to occur with great frequency and are spaced uniformly throughout the genome. The frequency and uniformity of SNPs means that there is a greater probability, compared with other types of polymorphisms such as tandem repeat polymorphisms, that a SNP will be found in close proximity to a genetic locus of interest. SNPs are also mutationally more stable than tandem repeat polymorphisms, such as VNTRs. LD-based association studies are capable of finding a disease susceptibility gene without any a priori assumptions about what or where the gene is.

Currently, however, it is not feasible to do SNP association studies over the entire human genome, therefore candidate genes associated with breast cancer are targeted for SNP identification and association analysis. The candidate gene approach uses a priori knowledge of disease pathogenesis to identify genes that are hypothesized to directly influence development of the disease. The candidate gene approach may focus on a gene that is directly targeted by a drug used to treat the disorder. To discover SNPs associated with an increased susceptibility to breast cancer candidate genes can be selected from systems physiologically implicated in the disease pathway. SNPs found in these genes are then tested for statistical association with disease in individuals who have the disease compared with appropriate controls. The candidate gene approach has the advantages of drastically reducing the number of candidate SNPs, and the number of individuals, that need to be typed, compared with LD-based association studies of random SNPs over large areas of, or complete, genomes. Furthermore, in the candidate gene approach, no assumptions are made about the extent of LD over any particular area of the genome.

Combined with the use of a high density map of appropriately spaced, sufficiently informative SNP markers, association studies, including linkage disequilibrium-based genome wide association studies, will enable the identification of most genes involved in complex disorders, such as cardiovascular diseases, cancer etc. This will enhance the selection of candidate genes most likely to contain causative SNPs associated with a particular disease. All of the SNPs disclosed by the present invention can be employed as part of genome-wide association studies or as part of candidate gene association studies. The present invention advances the state of the art and provides commercially useful embodiments by providing previously unidentified SNPs in the estrogen receptor genes.

SUMMARY OF THE INVENTION

The present invention is based on sequencing genomic DNA from human chromosome 6 and cDNAs to define the genomic structure of estrogen receptor beta genes, novel polymorphisms in the estrogen receptor gene/protein. Such polymorphisms can lead to a variety of disorders that are mediated/modulated by a variant estrogen receptor, such as a susceptibility to cancer, osteoporosis, cardiovascular disorders, etc. Based on this sequencing approach, the present invention provides genomic nucleotide sequences, cDNA sequences, amino acid sequences, sequence polymorphisms in the ESR-beta gene, methods of detecting these sequences/polymorphisms in a sample, methods of determining a risk of having or developing a disorder mediated by a variant estrogen receptor and methods of screening for compounds used to treat disorders mediated by a variant estrogen receptor.

DESCRIPTION OF THE FIGURES

FIG. 1 . Shows the Complete genomic sequence of the estrogen receptor beta gene.

FIG. 2 . Shows sequence polymorphisms found in the ESR-beta genomic DNA (nucleotide position is based on the sequence provided in FIGS. 1 and 3 ).

a) SNPs in Liverpool clinical tissue samples.

b) SNPs in pooled Coriell Diversity panels.

c) SNPs in unpooled Coriell Diversity panels.

d) SNPs in CEPH families.

e) PCR primers.

f) Sequencing primers.

FIG. 3 . Shows the nucleic acid sequence of a cDNA covering the ESR-beta gene.

FIG. 4 . Shows the amino acid sequence of the estrogen receptor beta protein.

FIG. 5. A graphic representation of the human ESR 2 locus.

(a) Complete structure of the human synaptic nuclei expressed gene 2 (syne-2) contained within intron 9 of ERβ. Exons are represented by filled boxes and introns by horizontal lines. Note that the gene is on the opposite strand as ERβ. (b) Complete structure of the human estrogen receptor beta (ERβ). Exons are represented by filled boxes and introns by horizontal lines. (c) Order and names of contigs used to complete the genomic sequence. GA numbers represent Celera contig numbers. Research genetics BAC clones are represented by standard plate and well numbering.

FIG. 6 . The exon structure of the genomic ESR-beta gene.

FIG. 7 . Shows the distribution and frequency of the SNPs of the present invention in the ESR-beta gene.

FIG. 8 . ESR-beta SNPs: a) in Coriell Samples, b) in Liverpool Samples (T=tumor sample, B=blood sample).

FIG. 9 . ESR-beta exons with SNPs. (see FIG. 2 for “N”, “C”, “I”, “A”, “S” representations). Underlined sequences indicate the primer sequences.

DETAILED DESCRIPTION OF THE INVENTION

General Description

The present invention is based on sequencing genomic DNA from human and cDNAs to define the genomic structure of estrogen receptor beta genes and novel polymorphisms in the estrogen receptor gene/protein. Such polymorphisms can lead to a variety of disorders that are mediated/modulated by a variant estrogen receptor, such as a susceptibility to cancer, osteoporosis, cardiovascular disorders, etc. Based on this sequencing approach, the present invention provides genomic nucleotide sequences, cDNA sequences, amino acid sequences and sequence polymorphisms in the ESR-beta gene, methods of detecting these sequences/polymorphisms in a sample, methods of determining a risk of having or developing a disorder mediated by a variant estrogen receptor and methods of screening for compounds used to treat disorders mediated by a variant estrogen receptor.

Isolated SNP-Containing Nucleic Acid Molecules

The present invention provides isolated nucleic acid molecules that contain one or more SNPs disclosed by the present invention. The present invention further provides isolated nucleic acid molecules that encode the variant protein. Such nucleic acid molecules will consist of, consist essentially of, or comprise one or more SNPs of the present invention. The nucleic acid molecule can have additional nucleic acid residues, such as nucleic acid residues that are naturally associated with it or heterologous nucleotide sequences.

As used herein, an “isolated” SNP-containing nucleic acid molecule is one that contains a SNP of the present invention and is separated from other nucleic acid present in the natural source of the nucleic acid. Generally, the isolated SNP-containing nucleic acid, as used herein, will be comprised of one or more SNP positions disclosed by the present invention with flanking nucleotide sequence on either side of the SNP positions. Preferably the flanking sequence is up to about 300 bases, 100 bases, 50 bases, 30 bases, 15 bases, 10 bases, or 4 bases on either side of a SNP position for detection reagents or as long as the entire protein encoding sequence if it is to be used to produce a protein containing the coding variants disclosed in Figures. The important point is that the nucleic acid is isolated from remote and unimportant flanking sequences and is of appropriate length such that it can be subjected to the specific manipulations or uses described herein such as recombinant expression, preparation of probes and primers for the SNP position, and other uses specific to the SNP-containing nucleic acid sequences.

Moreover, an “isolated” nucleic acid molecule, such as a cDNA molecule containing a SNP of the present invention, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. However, the nucleic acid molecule can be fused to other coding or regulatory sequences and still be considered isolated. For example, recombinant DNA molecules contained in a vector are considered isolated. Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA molecules in solution. Isolated RNA molecules include in vivo or in vitro RNA transcripts of the isolated SNP-containing DNA molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.

Isolated SNP-containing nucleic acid molecules can be in the form of RNA, such as mRNA, or in the form DNA, including cDNA and genomic DNA obtained by cloning or produced by chemical synthetic techniques or by a combination thereof. The nucleic acid, especially DNA, can be double-stranded or single-stranded. Single-stranded nucleic acid can be the coding strand (sense strand) or the non-coding strand (anti-sense strand).

The present invention further provides related nucleic acid molecules that hybridize under stringent conditions to the nucleic acid molecules disclosed herein. As used herein, the term “hybridizes under stringent conditions” is intended to describe conditions for hybridization and washing under which nucleotide sequences encoding a peptide at least 60-70% homologous to each other typically remain hybridized to each other. The conditions can be such that sequences at least about 60%, at least about 70%, or at least about 80%, or at least about 90% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology , John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. One example of stringent hybridization conditions are hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 5065° C. Examples of moderate to low stringency hybridization conditions are well known in the art.

Specific Embodiments

Peptide Molecules

The present invention provides nucleic acid sequences that encode variants of the estrogen receptor. These variant molecule/sequences will be referred to herein as the estrogen receptor variants of the present invention, the estrogen receptor proteins of the present invention, or peptides/proteins of the present invention.

The present invention provides isolated estrogen receptor protein molecules that consist of, consist essentially of or are comprised of the amino acid sequences of the estrogen receptor variant proteins disclosed herein.

As used herein, a protein or peptide is said to be “isolated” or “purified” when it is substantially free of cellular material or free of chemical precursors or other chemicals. The peptides of the present invention can be purified to homogeneity or other degrees of purity. The level of purification will be based on the intended use. The critical feature is that the preparation allows for the desired function of the peptide, even if in the presence of considerable amounts of other components.

In some uses, “substantially free of cellular material” includes preparations of the peptide having less than about 30% (by dry weight) other proteins (i.e., contaminating protein), less than about 20% other proteins, less than about 10% other proteins, or less than about 5% other proteins. When the peptide is recombinantly produced, it can also be substantially free of culture medium, i.e., culture medium represents less than about 20% of the volume of the protein preparation.

The language “substantially free of chemical precursors or other chemicals” includes preparations of the peptide in which it is separated from chemical precursors or other chemicals that are involved in its synthesis. In one embodiment, the language “substantially free of chemical precursors or other chemicals” includes preparations of the estrogen receptor protein having less than about 30% (by dry weight) chemical precursors or other chemicals, less than about 20% chemical precursors or other chemicals, less than about 10% chemical precursors or other chemicals, or less than about 5% chemical precursors or other chemicals.

The isolated estrogen receptor proteins can be purified from cells that naturally express it, purified from cells that have been altered to express it (recombinant), or synthesized using known protein synthesis methods. For example, a nucleic acid molecule encoding the estrogen receptor protein is cloned into an expression vector, the expression vector introduced into a host cell and the protein expressed in the host cell. The protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Many of these techniques are described in detail below.

Accordingly, the present invention provides proteins that are encoded by an ESR-beta gene that consist of the ESR-beta encoding nucleic acid sequence provided in FIGS. 1 and 3 , including one or more of the sequence polymorphisms provided in FIG. 2. A protein consists of an amino acid sequence when the amino acid sequence is the final amino acid sequence of the protein.

The present invention further provides proteins that are encoded by an ESR-beta gene that consist essentially of the ESR-beta encoding nucleic acid sequence provided in FIGS. 1 and 3 , including one or more of the sequence polymorphisms provided in FIG. 2. A protein consists essentially of an amino acid sequence when such an amino acid sequence is present with only a few additional amino acid residues in the final protein.

The present invention further provides a that are encoded by an ESR-beta gene that comprises the ESR-beta encoding nucleic acid sequence provided in FIGS. 1 and 3 , including one or more of the sequence polymorphisms provided in FIG. 2. A protein is comprised of an amino acid sequence when the amino acid sequence is at least part of the final amino acid sequence of the protein. In such a fashion, the protein can be only the peptide or have additional amino acid molecules, such as amino acid residues (contiguous encoded sequence) that are naturally associated with it or heterologous amino acid residues/peptide sequences. Such a protein can have a few additional amino acid residues or can comprise several hundred or more additional amino acids. A brief description of how various types of these proteins can be made/isolated is provided below.

The estrogen receptor protein of the present invention can be attached to heterologous sequences to form chimeric or fusion proteins. Such chimeric and fusion proteins comprise a estrogen receptor protein operatively linked to a heterologous protein having an amino acid sequence not substantially homologous to the estrogen receptor protein. “Operatively linked” indicates that the estrogen receptor protein and the heterologous protein are fused in-frame. The heterologous protein can be fused to the N-terminus or C-terminus of the estrogen receptor protein.

In some uses, the fusion protein does not affect the activity of the estrogen receptor protein per se. For example, the fusion protein can include, but is not limited to, enzymatic fusion proteins, for example beta-galactosidase fusions, yeast two-hybrid GAL fusions, poly-His fusions, MYC-tagged, FE-tagged and Ig fusions. Such fusion proteins, particularly poly-His fusions, can facilitate the purification of recombinant estrogen receptor protein. In certain host cells (e.g., mammalian host cells), expression and/or secretion of a protein can be increased by using a heterologous signal sequence.

A chimeric or fusion protein can be produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different protein sequences are ligated together in-frame in accordance with conventional techniques. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and re-amplified to generate a chimeric gene sequence (see Ausubel et al., Current Protocols in Molecular Biology, 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety (e.g., a GST protein). A estrogen receptor protein-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the estrogen receptor protein.

Polypeptides often contain amino acids other than the 20 amino acids commonly referred to as the 20 naturally-occurring amino acids. Further, many amino acids, including the terminal amino acids, may be modified by natural processes, such as processing and other post-translational modifications, or by chemical modification techniques well known in the art. Common modifications that occur naturally in polypeptides are described in basic texts, detailed monographs, and the research literature, and they are well known to those of skill in the art. Accordingly, the polypeptides also encompass derivatives or analogs in which a substituted amino acid residue is not one encoded by the genetic code, in which a substituent group is included, in which the mature polypeptide is fused with another compound, such as a compound to increase the half-life of the polypeptide (for example, polyethylene glycol), or in which the additional amino acids are fused to the mature polypeptide, such as a leader or secretory sequence or a sequence for purification of the mature polypeptide or a pro-protein sequence.

Known modifications include, but are not limited to, acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent crosslinks, formation of cystine, formation of pyroglutamate, formylation, gamma carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination.

Such modifications are well-known to those of skill in the art and have been described in great detail in the scientific literature. Several particularly common modifications, glycosylation, lipid attachment, sulfation, gamma-carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation, for instance, are described in most basic texts, such as Proteins—Structure and Molecular Properties, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York (1993). Many detailed reviews are available on this subject, such as by Wold, F., Post - translational Covalent Modification of Proteins , B. C. Johnson, Ed., Academic Press, New York 1-12 (1983); Seifter et al. ( Meth Enzymol. 182: 626-646 (1990)) and Rattan et al. ( Ann. N.Y. Acad. Sci. 663:48-62 (1992)).

The present invention further provides fragments of the estrogen receptor proteins of the present invention, in addition to proteins and peptides that comprise and consist of such fragments. The fragments to which the invention pertains, however, are not to be construed as encompassing fragments that may be disclosed publicly prior to the present invention.

As used herein, a fragment comprises at least 8 or more contiguous amino acid residues from a estrogen receptor protein. Such fragments can be chosen based on the ability to retain one or more of the biological activities of the estrogen receptor protein or could be chosen for the ability to perform a function, e.g. act as an immunogen. Particularly important fragments are biologically active fragments, peptides which are, for example, about 8 or more amino acids in length, that contain a variant amino acid residue (FIG. 2 ). Such fragments will typically comprise a domain or motif of the estrogen receptor proteins of the present invention, e.g., active site, ligand binding domain or DNA binding domain. Further, possible fragments include, but are not limited to, domain or motif containing fragments, soluble peptide fragments, and fragments containing immunogenic structures. Predicted domains and functional sites are readily identifiable by computer programs well-known and readily available to those of skill in the art (e.g., PROSITE analysis).

Protein/Peptide Uses

The proteins of the present invention can be used in assays to determine the biological activity of the protein, including in a panel of multiple proteins for high-throughput screening; to raise antibodies or to elicit another immune response; as a reagent (including the labeled reagent) in assays designed to quantitatively determine levels of the protein (or its binding partner or receptor) in biological fluids; and as markers for tissues in which the corresponding protein is preferentially expressed (either constitutively or at a particular stage of tissue differentiation or development or in a disease state). Any or all of these research utilities are capable of being developed into reagent grade or kit format for commercialization as research products. Methods for performing the uses listed above are well known to those skilled in the art. References disclosing such methods include “Molecular Cloning: A Laboratory Manual”, 2d ed., Cold Spring Harbor Laboratory Press, Sambrook, J., E. F. Fritsch and T. Maniatis eds., 1989, and “Methods in Enzymology: Guide to Molecular Cloning Techniques”, Academic Press, Berger, S. L. and A. R. Kimmel eds., 1987.

The estrogen receptor proteins of the present invention are useful for biological assay. Such assays involve any of the known estrogen receptor functions or activities or properties useful for the diagnosis and treatment of estrogen receptor-related conditions (see Background Section).

The estrogen receptor proteins of the present invention are also useful in drug screening assays, such as in cell-based or cell-free systems. Cell-based systems can be native, i.e., cells that normally express the receptor protein, as a biopsy or expanded in cell culture. In one embodiment, however, cell-based assays involve recombinant host cells expressing the receptor protein.

The estrogen receptor proteins of the present invention can be used to identify compounds that modulate receptor activity. Both the estrogen receptor protein of the present invention and appropriate fragments can be used in high-throughput screens to assay candidate compounds for the ability to bind and/or modulate the activity of the receptor. These compounds can be further screened against a functional receptor to determine the effect of the compound on the receptor activity. Further, these compounds can be tested in animal or invertebrate systems to determine activity/effectiveness. Compounds can be identified that activate (agonist) or inactivate (antagonist) the receptor to a desired degree. Such compounds can be selected for the ability to act on one or more of the variant estrogen receptor proteins of the present invention.

Further, the receptor polypeptides can be used to screen a compound for the ability to stimulate or inhibit interaction between the receptor protein and a target molecule that normally interacts with the receptor protein, e.g. estrogen. The target can be ligand or a binding partner that the receptor protein normally interacts (for example, an estrogen ligand or a DNA sequence). Such assays typically include the steps of combining the receptor protein with a candidate compound under conditions that allow the receptor protein, or fragment, to interact with the target molecule, and to detect the formation of a complex between the protein and the target or to detect the biochemical consequence of the interaction with the receptor protein and the target, such as any of the associated effects of DNA binding or signal transduction.

Candidate compounds include, for example, 1) peptides such as soluble peptides, including Ig-tailed fusion peptides and members of random peptide libraries (see, e.g., Lam et al., Nature 354:82-84 (1991); Houghten et al., Nature 354:84-86 (1991)) and combinatorial chemistry-derived molecular libraries made of D- and/or L-configuration amino acids; 2) phosphopeptides (e.g., members of random and partially degenerate, directed phosphopeptide libraries, see, e.g., Songyang et al., Cell 72:767-778 (1993)); 3) antibodies (e.g., polyclonal, monoclonal, humanized, anti-idiotypic, chimeric, and single chain antibodies as well as Fab, F(ab′) 2 , Fab expression library fragments, and epitope-binding fragments of antibodies); and 4) small organic and inorganic molecules (e.g., molecules obtained from combinatorial and natural product libraries).

One candidate compound is a soluble fragment of the receptor that competes for ligand binding. Other candidate compounds include mutant receptors or appropriate fragments containing mutations that affect receptor function and thus compete for ligand. Accordingly, a fragment that competes for ligand, for example with a higher affinity, or a fragment that binds ligand but does not allow release, is encompassed by the invention.

The invention further includes other end point assays to identify compounds that modulate (stimulate or inhibit) receptor activity. The assays typically involve an assay of events in the signal transduction pathway that indicate receptor activity. Thus, the expression of genes that are up- or down-regulated in response to the receptor protein dependent signal cascade can be assayed. In one embodiment, the regulatory region of such genes can be operably linked to a marker that is easily detectable, such as luciferase. Alternatively, phosphorylation of the receptor protein, or a receptor protein target, could also be measured. Any of the biological or biochemical functions mediated by the receptor can be used as an endpoint assay. These include all of the biochemical or biochemical/biological events described herein, in the references cited herein, incorporated by reference for these endpoint assay targets, and other functions known to those of ordinary skill in the art.

The receptor polypeptides are also useful in competition binding assays in methods designed to discover compounds that interact with the receptor. Thus, a compound is exposed to a receptor polypeptide under conditions that allow the compound to bind or to otherwise interact with the polypeptide. Ligands to the receptor are also added to the mixture. If the test compound interacts with the receptor or ligand, it decreases the amount of complex formed or activity from the receptor target. This type of assay is particularly useful in cases in which compounds are sought that interact with specific regions of the receptor.

To perform cell free drug screening assays, it is sometimes desirable to immobilize either the receptor protein, or fragment, or its target molecule to facilitate separation of complexes from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay.

Techniques for immobilizing proteins on matrices can be used in the drug screening assays. In one embodiment, a fusion protein can be provided which adds a domain that allows the protein to be bound to a matrix. For example, glutathione-S-transferase fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, Mo.) or glutathione derivatized microtitre plates, which are then combined with the cell lysates (e.g., 35 S-labeled) and the candidate compound, and the mixture incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads are washed to remove any unbound label, and the matrix immobilized and radiolabel determined directly, or in the supernatant after the complexes are dissociated. Alternatively, the complexes can be dissociated from the matrix, separated by SDS-PAGE, and the level of receptor-binding protein found in the bead fraction quantitated from the gel using standard electrophoretic techniques. For example, either the polypeptide or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin using techniques well known in the art. Alternatively, antibodies reactive with the protein but which do not interfere with binding of the protein to its target molecule can be derivatized to the wells of the plate, and the protein trapped in the wells by antibody conjugation. Preparations of a receptor-binding protein and a candidate compound are incubated in the receptor protein-presenting wells and the amount of complex trapped in the well can be quantitated. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the receptor protein target molecule, or which are reactive with receptor protein and compete with the target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the target molecule.

Agents that modulate the protein of the present invention can be identified using one or more of the above assays, alone or in combination. It is generally preferable to use a cell-based or cell free system first and then confirm activity in an animal or other model system. Such model systems are well known in the art and can readily be employed in this context.

Modulators of receptor protein activity identified according to these drug screening assays can be used to treat a subject with a disorder mediated by the receptor pathway, by treating cells that express the estrogen receptor protein. These methods of treatment include the steps of administering the modulators of protein activity in a pharmaceutical composition as described herein, to a subject in need of such treatment.

This invention further pertains to novel agents identified by the above-described screening assays. Accordingly, it is within the scope of this invention to further use an agent identified as described herein in an appropriate animal model. For example, an agent identified as described herein (e.g., an estrogen receptor modulating agent, an antisense estrogen receptor nucleic acid molecule, an estrogen receptor-specific antibody, or an estrogen receptor-binding partner) can be used in an animal model to determine the efficacy, toxicity, or side effects of treatment with such an agent. Alternatively, an agent identified as described herein can be used in an animal model to determine the mechanism of action of such an agent. Furthermore, this invention pertains to uses of novel agents identified by the above-described screening assays for treatments as described herein.

The estrogen receptor proteins of the present invention are also useful to provide a a=target for diagnosing a disease or predisposition to disease mediated by the estrogen receptor. Accordingly, the invention provides methods for detecting the presence, or levels of, the estrogen receptor variants of the present invention (or encoding mRNA) in a cell, tissue, or organism. The method involves contacting a biological sample with a compound capable of interacting with the receptor protein (or gene or mRNA encoding the receptor) such that the interaction can be detected.

One agent for detecting a protein in a sample is an antibody capable of selectively binding to a variant form of the estrogen receptor protein. Such samples include tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject. Other agents, such as nucleic acid based probes, primers and arrays are described below.

The estrogen receptor proteins of the present invention also provide targets for diagnosing active disease, or predisposition to disease, in a patient having a variant estrogen receptor, particularly a disease involving the estrogen pathway, such as cardiovascular disease, autoimmune disease, cell differentiation, etc (see Background Section). Thus, the receptor can be isolated from a biological sample and assayed for the presence of a genetic mutation that results in aberrant receptor activity. This includes amino acid substitution, deletion, insertion, rearrangement, (as the result of aberrant splicing events), and inappropriate post-translational modification as provided in FIG. 2 . Analytic methods include altered electrophoretic mobility, altered tryptic peptide digest, altered receptor activity in cell-based or cell-free assay, alteration in ligand or antibody-binding pattern, altered isoelectric point, direct amino acid sequencing, and any other of the known assay techniques useful for detecting mutations in a protein. Particularly useful are the variants provided in FIG. 2 .

In vitro techniques for detection of peptide include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations and immunofluorescence. Alternatively, the peptide can be detected in vivo in a subject by introducing into the subject a labeled anti-peptide antibody. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques. Particularly useful are methods that detect the specific allelic variants of the estrogen receptor disclosed herein that are expressed in a subject and methods which detect fragments of a peptide in a sample.

The peptides are also useful in pharmacogenomic analysis. Pharmacogenomics deal with clinically significant hereditary variations in the response to drugs due to altered drug disposition and abnormal action in affected persons. See, e.g., Eichelbaum, M. ( Clin Exp. Pharmacol. Physiol. 23(10-11):983-985 (1996)), and Linder, M. W. ( Clin Chem. 43(2):254-266 (1997)). The clinical outcomes of these variations result in severe toxicity of therapeutic drugs in certain individuals or therapeutic failure of drugs in certain individuals as a result of individual variation in metabolism. Thus, the genotype of the individual can determine the way a therapeutic compound acts on the body or the way the body metabolizes the compound. Further, the activity of drug metabolizing enzymes effects both the intensity and duration of drug action. Thus, the pharmacogenomics of the individual permit the selection of effective compounds and effective dosages of such compounds for prophylactic or therapeutic treatment based on the individual's genotype. The discovery of genetic polymorphisms in some drug metabolizing enzymes has explained why some patients do not obtain the expected drug effects, show an exaggerated drug effect, or experience serious toxicity from standard drug dosages. Polymorphisms can be expressed in the phenotype of the extensive metabolizer and the phenotype of the poor metabolizer. Accordingly, genetic polymorphism may lead to allelic protein variants of the receptor protein in which one or more of the receptor functions in one population is different from those in another population. The peptides thus allow a target to ascertain a genetic predisposition that can affect treatment modality. Thus, in a ligand-based treatment, polymorphism may give rise to amino terminal extracellular domains and/or other ligand-binding regions that are more or less active in ligand binding, and receptor activation. Accordingly, ligand dosage would necessarily be modified to maximize the therapeutic effect within a given population containing a polymorphism/haplotype. As an alternative to genotyping, specific polymorphic peptides could be identified.

Antibodies

The invention also provides antibodies that selectively bind to the estrogen receptor proteins of the present invention as well as fragments thereof. As used herein, an antibody selectively binds a target protein when it binds the target protein and does not significantly bind to unrelated proteins. An antibody is still considered to selectively bind a protein even if it also binds to other proteins that are not substantially homologous with the target protein so long as such proteins share homology with a fragment or domain of the protein target of the antibody. In this case, it would be understood that antibody binding to the protein is still selective despite some degree of cross-reactivity.

As used herein, an antibody is defined in terms consistent with that recognized within the art: they are multi-subunit proteins produced by a mammalian organism in response to an antigen challenge. The antibodies of the present invention include polyclonal antibodies and monoclonal antibodies, as well as fragments of such antibodies, including, but not limited to, Fab or F(ab′) 2 , and Fv fragments.

Many methods are known for generating and/or identifying antibodies to a given target peptide. Several such methods are described by Harlow, Antibodies, Cold Spring Harbor Press, (1989). In general, to generate antibodies, an isolated peptide is used as an immunogen and is administered to a mammalian organism, such as a rat, rabbit or mouse. The full-length protein, an antigenic peptide fragment or a fusion protein can be used.

Antibodies are preferably prepared from regions or discrete fragments of the estrogen receptor protein. Antibodies can be prepared from any region of the peptide as described herein. However, preferred regions will include amino acids residues that are altered in the variants of the present inventions, particularly those involved in function/activity and/or receptor/binding partner interaction. An antigenic fragment will typically comprise at least 10 contiguous amino acid residues. The antigenic peptide can comprise, however, at least 12, 14, 20 or more amino acid residues. Such fragments can be selected on a physical property, such as fragments correspond to regions that are located on the surface of the protein, e.g., hydrophilic regions or can be selected based on sequence uniqueness.

Detection on an antibody of the present invention can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, O-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include 125 I, 131 I, 35 S or 3 H.

Antibody Uses

The antibodies can be used to isolate the estrogen receptor protein of the present invention by standard techniques, such as affinity chromatography or immunoprecipitation. The antibodies can facilitate the purification of the natural protein from cells and recombinantly produced protein expressed in host cells. In addition, such antibodies are useful to detect the presence of the estrogen receptor protein of the present invention in cells or tissues to determine the pattern of expression of the protein among various tissues in an organism and over the course of normal development. Further, such antibodies can be used to detect protein in situ, in vitro, or in a cell lysate or supernatant in order to evaluate the abundance and pattern of expression. Also, such antibodies can be used to assess abnormal tissue distribution or abnormal expression during development. Antibody detection of circulating fragments of the full length estrogen receptor protein can be used to identify turnover.

Further, the antibodies can be used to assess expression in disease states such as in active stages of the disease or in an individual with a predisposition toward disease related to the protein's function, particularly diseases involving cardiovascular disease, autoimmune disease, etc (see Background Section). When a disorder is caused by an inappropriate tissue distribution, developmental expression, level of expression of the protein, or expressed/processed form, the antibody can be prepared against the normal protein. If a disorder is characterized by a specific mutation in the protein, antibodies specific for this mutant protein can be used to assay for the presence of the specific mutant protein.

The antibodies can also be used to assess normal and aberrant subcellular localization of cells in the various tissues in an organism. The diagnostic uses can be applied, not only in genetic testing, but also in monitoring a treatment modality.

Accordingly, where treatment is ultimately aimed at correcting the expression level or the presence of aberrant sequence and aberrant tissue distribution or developmental expression, antibodies directed against the protein or relevant fragments can be used to monitor therapeutic efficacy.

Additionally, antibodies are useful in pharmacogenomic analysis. Thus, antibodies prepared against polymorphic proteins can be used to identify individuals that require modified treatment modalities. The antibodies are also useful as diagnostic tools as an immunological marker for aberrant estrogen receptor protein analyzed by electrophoretic mobility, isoelectric point, tryptic peptide digest, and other physical assays known to those in the art.

The antibodies are also useful for inhibiting protein function, for example, blocking the binding of the estrogen receptor protein to a binding partner such as a ligand. These uses can also be applied in a therapeutic context in which treatment involves inhibiting the protein's function. An antibody can be used, for example, to block binding, thus modulating (agonizing or antagonizing) the peptides activity. Antibodies can be prepared against specific fragments containing sites required for function or against intact protein that is associated with a cell or cell membrane.

The invention also encompasses kits for using antibodies to detect the presence of a protein in a biological sample. The kit can comprise antibodies such as a labeled or labelable antibody and a compound or agent for detecting estrogen receptor protein in a biological sample; means for determining the amount of protein in the sample; means for comparing the amount of estrogen receptor protein in the sample with a standard; and instructions for use.

Nucleic Acid Molecules

The present invention further provides isolated nucleic acid molecules that encode any of the estrogen receptor proteins of the present invention. Such nucleic acid molecules will consist of, consist essentially of, or comprise a nucleotide sequence that encodes one of the estrogen receptor proteins of the present invention.

As used herein, an “isolated” nucleic acid molecule is one that is separated from other nucleic acid present in the natural source of the nucleic acid. Preferably, an “isolated” nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5′ and 3′ ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. However, there can be some flanking nucleotide sequences, for example up to about 5 KB, 4 KB, 3 KB, 2 KB, or 1 KB or less, particularly contiguous peptide encoding sequences and peptide encoding sequences within the same gene but separated by introns in the genomic sequence. The important point is that the nucleic acid is isolated from remote and unimportant flanking sequences such that it can be subjected to the specific manipulations described herein such as recombinant expression, preparation of probes and primers, and other uses specific to the nucleic acid sequences.

Moreover, an “isolated” nucleic acid molecule, such as a cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. However, the nucleic acid molecule can be fused to other coding or regulatory sequences and still be considered isolated.

For example, recombinant DNA molecules contained in a vector are considered isolated. Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA molecules in solution. Isolated RNA molecules include in vivo or in vitro RNA transcripts of the isolated DNA molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.

Accordingly, the present invention provides nucleic acid molecules that consist of the ESR-beta encoding nucleotide sequence shown in FIGS. 1 and 3 , including one or more of the sequence polymorphisms provided in FIG. 2. A nucleic acid molecule consists of a nucleotide sequence when the nucleotide sequence is the complete nucleotide sequence of the nucleic acid molecule.

The present invention further provides nucleic acid molecules that consist essentially of the ESR-beta encoding nucleotide sequence shown in FIGS. 1 and 3 , including one or more of the sequence polymorphisms provided in FIG. 2. A nucleic acid molecule consists essentially of a nucleotide sequence when such a nucleotide sequence is present with only a few additional nucleic acid residues in the final nucleic acid molecule.

The present invention further provides nucleic acid molecules that are comprised of the ESR-beta encoding nucleotide sequences shown in FIGS. 1 and 3 , including one or more of the sequence polymorphisms provided in FIG. 2. A nucleic acid molecule is comprised of a nucleotide sequence when the nucleotide sequence is at least part of the final nucleotide sequence of the nucleic acid molecule. In such a fashion, the nucleic acid molecule can be only the nucleotide sequence or have additional nucleic acid residues, such as nucleic acid residues that are naturally associated with it or heterologous nucleotide sequences. Such a nucleic acid molecule can have a few additional nucleotides or can comprise several hundred or more additional nucleotides. A brief description of how various types of these nucleic acid molecules can be readily made/isolated is provided below.

The isolated nucleic acid molecules can encode the mature protein plus additional amino or carboxyl-terminal amino acids, or amino acids interior to the mature peptide (when the mature form has more than one peptide chain, for instance). Such sequences may play a role in processing of a protein from precursor to a mature form, facilitate protein trafficking, prolong or shorten protein half-life or facilitate manipulation of a protein for assay or production, among other things. As generally is the case in situ, the additional amino acids may be processed away from the mature protein by cellular enzymes.

As mentioned above, the isolated nucleic acid molecules include, but are not limited to, the sequence encoding the estrogen receptor protein alone, the sequence encoding the mature peptide and additional coding sequences, such as a leader or secretory sequence (e.g., a pre-pro or pro-protein sequence), the sequence encoding the mature peptide, with or without the additional coding sequences, plus additional non-coding sequences, for example introns and non-coding 5′ and 3′ sequences such as transcribed but non-translated sequences that play a role in transcription, mRNA processing (including splicing and polyadenylation signals), ribosome binding and stability of mRNA, as well as genomic regulatory sequences such as promoters. In addition, the nucleic acid molecule may be fused to a marker sequence encoding, for example, a peptide that facilitates purification.

Isolated nucleic acid molecules can be in the form of RNA, such as mRNA, or in the form DNA, including cDNA and genomic DNA obtained by cloning or produced by chemical synthetic techniques or by a combination thereof. The nucleic acid, especially DNA, can be double-stranded or single-stranded. Single-stranded nucleic acid can be the coding strand (sense strand) or the noncoding strand (anti-sense strand).

The invention further provides nucleic acid molecules that encode fragments of the proteins of the present invention. A fragment comprises a contiguous nucleotide sequence greater than 12 or more nucleotides. Further, a fragment could at least 30, 40, 50, 100, 250 or 500 nucleotides in length. The length of the fragment will be based on its intended use. For example, the fragment can encode epitope bearing regions of the peptide, or can be useful as DNA probes and primers. Such fragments can be isolated using the known nucleotide sequence to synthesize an oligonucleotide probe. A labeled probe can then be used to screen a cDNA library, genomic DNA library, or mRNA to isolate nucleic acid corresponding to the coding region. Further, primers can be used in PCR reactions to clone specific regions of gene.

A probe/primer typically comprises substantially a purified oligonucleotide or oligonucleotide pair. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 20, 25, 40, 50 or more consecutive nucleotides. The preferred probe/primer set will cover/span one of the variant bases identified in FIG. 2 .

As used herein, the term “hybridizes under stringent conditions” is intended to describe conditions for hybridization and washing under which nucleotide sequences encoding a peptide at least 50-55% homologous to each other typically remain hybridized to each other. The conditions can be such that sequences at least about 65%, at least about 70%, or at least about 75% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology , John Wiley & Sons, N.Y. (1989), Sections 6.3.1-6.3.6. One example of stringent hybridization conditions are hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50-65C.

Nucleic Acid Molecule Uses

The nucleic acid molecules of the present invention are useful for probes, primers, chemical intermediates, and in biological assays. The probe can correspond to any sequence along the entire length of the nucleic acid molecules provided in FIGS. 1 and 3 , including one or more of the sequence polymorphisms provided in FIG. 2 . Accordingly, it could be derived from 5′ noncoding regions, the coding region, and 3′ noncoding regions. However, as discussed, fragments are not to be construed as encompassing fragments disclosed prior to the present invention.

The nucleic acid molecules are also useful as primers for PCR to amplify any given region of a nucleic acid molecule and are useful to synthesize antisense molecules of desired length and sequence.

The nucleic acid molecules are also useful for constructing recombinant vectors. Such vectors include expression vectors that express a portion of, or all of, the peptide sequences. Vectors also include insertion vectors, used to integrate into another nucleic acid molecule sequence, such as into the cellular genome, to alter in situ expression of a gene and/or gene product. For example, an endogenous coding sequence can be replaced via homologous recombination with all or part of the coding region containing one or more specifically introduced mutations.

The nucleic acid molecules are also useful for expressing antigenic portions of the proteins.

The nucleic acid molecules are also useful for designing ribozymes corresponding to all, or a part, of the mRNA produced from the nucleic acid molecules described herein.

The nucleic acid molecules are also useful for constructing host cells expressing a part, or all, of the nucleic acid molecules and peptides.

The nucleic acid molecules are also useful for constructing transgenic animals expressing all, or a part, of the nucleic acid molecules and peptides.

The nucleic acid molecules are also useful for making vectors that express part, or all, of the peptides.

The nucleic acid molecules are also useful as hybridization probes for determining the presence, level, form and distribution of nucleic acid expression. Accordingly, the probes can be used to detect the presence of, or to determine levels of, a specific nucleic acid molecule in cells, tissues, and in organisms. The nucleic acid whose level is determined can be DNA or RNA. Accordingly, probes corresponding to the peptides described herein can be used to assess expression and/or gene copy number in a given cell, tissue, or organism. These uses are relevant for diagnosis of disorders involving an increase or decrease in estrogen receptor protein expression relative to normal results.

In vitro techniques for detection of mRNA include Northern hybridizations, in situ hybridizations and nucleic acid array based expression profiling. In vitro techniques for detecting DNA includes Southern hybridizations, in situ hybridization and nucleic acid array based expression profiling.

Probes can be used as a part of a diagnostic test kit or nucleic acid array format for identifying cells or tissues that express a estrogen receptor proteins of the present invention, such-as by measuring a level of a receptor-encoding nucleic acid in a sample of cells from a subject e.g., mRNA or genomic DNA, or determining if a receptor gene has been mutated.

Nucleic acid expression assays are useful for drug screening to identify compounds that modulate estrogen receptor nucleic acid expression.

The invention thus provides a method for identifying a compound that can be used to treat a disorder associated with nucleic acid expression of the estrogen receptor gene. The method typically includes assaying the ability of the compound to modulate the expression of the estrogen receptor nucleic acid and thus identifying a compound that can be used to treat a disorder characterized by undesired estrogen receptor nucleic acid expression. The assays can be performed in cell-based and cell-free systems. Cell-based assays include cells naturally expressing the estrogen receptor nucleic acid or recombinant cells genetically engineered to express specific nucleic acid sequences.

The assay for estrogen receptor nucleic acid expression can involve direct assay of nucleic acid levels, such as mRNA levels, or on collateral compounds involved in the signal pathway. Further, the expression of genes that are up- or down-regulated in response to the estrogen receptor protein signal pathway can also be assayed. In this embodiment the regulatory regions of these genes can be operably linked to a reporter gene such as luciferase.

Thus, modulators of estrogen receptor gene expression can be identified in a method wherein a cell is contacted with a candidate compound and the expression of mRNA determined. The level of expression of estrogen receptor mRNA in the presence of the candidate compound is compared to the level of expression of estrogen receptor mRNA in the absence of the candidate compound. The candidate compound can then be identified as a modulator of nucleic acid expression based on this comparison and be used, for example to treat a disorder characterized by aberrant nucleic acid expression. When expression of mRNA is statistically significantly greater in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of nucleic acid expression. When nucleic acid expression is statistically significantly less in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of nucleic acid expression.

The invention further provides methods of treatment, with the nucleic acid as a target, using a compound identified through drug screening as a gene modulator to modulate estrogen receptor nucleic acid expression. Modulation includes both up-regulation (i.e. activation or agonization) or down-regulation (suppression or antagonization) or nucleic acid expression.

Alternatively, a modulator for estrogen receptor nucleic acid expression can be a small molecule or drug identified using the screening assays described herein as long as the drug or small molecule inhibits the estrogen receptor nucleic acid expression.

The nucleic acid molecules are also useful for monitoring the effectiveness of modulating compounds on the expression or activity of the estrogen receptor gene in clinical trials or in a treatment regimen. Thus, the gene expression pattern can serve as a barometer for the continuing effectiveness of treatment with the compound, particularly with compounds to which a patient can develop resistance. The gene expression pattern can also serve as a marker indicative of a physiological response of the affected cells to the compound. Accordingly, such monitoring would allow either increased administration of the compound or the administration of alternative compounds to which the patient has not become resistant. Similarly, if the level of nucleic acid expression falls below a desirable level, administration of the compound could be commensurately decreased.

The nucleic acid molecules are also useful in diagnostic assays for qualitative changes in estrogen receptor nucleic acid, and particularly in qualitative changes that lead to pathology. The nucleic acid molecules can be used to detect mutations in estrogen receptor genes and gene expression products such as mRNA. The nucleic acid molecules can be used as hybridization probes to detect naturally-occurring genetic mutations in the estrogen receptor gene and thereby to determine whether a subject with the mutation is at risk for a disorder caused by the mutation. Mutations include deletion, addition, or substitution of one or more nucleotides in the gene, particularly those identified in FIG. 2 , and chromosomal rearrangement, such as inversion or transposition, modification of genomic DNA, such as aberrant methylation patterns or changes in gene copy number, such as amplification. Detection of a mutated form of the estrogen receptor gene associated with a dysfunction provides a diagnostic tool for an active disease or susceptibility to disease when the disease results from overexpression, underexpression, or altered expression of a estrogen receptor protein.

Individuals carrying mutations in the estrogen receptor gene can be detected at the nucleic acid level by a variety of techniques. Genomic DNA can be analyzed directly or can be amplified by using PCR prior to analysis. RNA or cDNA can be used in the same way. Samples can be used to examine one variation at a time or multiplexed, such as by using a nucleic acid array, to examine many variants in a single experiment. In some uses, detection of the mutation involves the use of a probe/primer in a polymerase chain reaction (PCR) (see, e.g. U.S. Pat. Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or, alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegran et al., Science 241:1077-1080 (1988); and Nakazawa et al., PNAS 91:360-364 (1994)), the latter of which can be particularly useful for detecting point mutations in the gene (see Abravaya et al., Nucleic Acids Res. 23:675-682 (1995)). This method can include the steps of collecting a sample of cells from a patient, isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, contacting the nucleic acid sample with one or more primers which specifically hybridize to a gene under conditions such that hybridization and amplification of the gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. Deletions and insertions can be detected by a change in size of the amplified product compared to the normal genotype. Point mutations can be identified by hybridizing amplified DNA to normal RNA or antisense DNA sequences.

Alternatively, mutations in a estrogen receptor gene can be directly identified, for example, by alterations in restriction enzyme digestion patterns determined by gel electrophoresis.

Further, sequence-specific ribozymes (U.S. Pat. No. 5,498,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site. Perfectly matched sequences can be distinguished from mismatched sequences by nuclease cleavage digestion assays or by differences in melting temperature.

Sequence changes at specific locations can also be assessed by nuclease protection assays such as RNase and S1 protection or the chemical cleavage method. Furthermore, sequence differences between a mutant estrogen receptor gene and a wild-type gene can be determined by direct DNA sequencing. A variety of automated sequencing procedures can be utilized when performing the diagnostic assays ((1995) Biotechniques 19:448), including sequencing by mass spectrometry (see, e.g., PCT International Publication No. WO 94/16101; Cohen et al., Adv. Chromatogr. 36:127-162 (1996); and Griffin et al., Appl. Biochem. Biotechnol. 38:147-159 (1993)).

Other methods for detecting mutations in the gene include methods in which protection from cleavage agents is used to detect mismatched bases in RNA/RNA or RNA/DNA duplexes (Myers et al., Science 230:1242 (1985)); Cotton et al., PNAS 85:4397 (1988); Saleeba et al., Meth Enzymol. 217:286295 (1992)), electrophoretic mobility of mutant and wild type nucleic acid is compared (Orita et al., PNAS 86:2766 (1989); Cotton et al., Mutat. Res. 285:125-144 (1993); and Hayashi et al., Genet. Anal. Tech Appl. 9:73-79 (1992)), and movement of mutant or wild-type fragments in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (Myers et al., Nature 313:495 (1985)). Examples of other techniques for detecting point mutations include, selective oligonucleotide hybridization, selective amplification, and selective primer extension.

The nucleic acid molecules are also useful for testing an individual for a genotype that while not necessarily causing the disease, nevertheless affects the treatment modality. Thus, the nucleic acid molecules can be used to study the relationship between an individual's genotype and the individual's response to a compound used for treatment (pharmacogenomic relationship). Accordingly, the nucleic acid molecules described herein can be used to assess the mutation content of the estrogen receptor gene in an individual in order to select an appropriate compound or dosage regimen for treatment.

Thus nucleic acid molecules displaying genetic variations that affect treatment provide a diagnostic target that can be used to tailor treatment in an individual. Accordingly, the production of recombinant cells and animals containing these polymorphism allow effective clinical design of treatment compounds and dosage regimens.

The nucleic acid molecules are thus useful as antisense constructs to control estrogen receptor gene expression in cells, tissues, and organisms. A DNA antisense nucleic acid molecule is designed to be complementary to a region of the gene involved in transcription, preventing transcription and hence production of estrogen receptor protein. An antisense RNA or DNA nucleic acid molecule would hybridize to the mRNA and thus block translation of mRNA into estrogen receptor protein.

Alternatively, a class of antisense molecules can be used to inactivate mRNA in order to decrease expression of estrogen receptor nucleic acid. Accordingly, these molecules can treat a disorder characterized by abnormal or undesired estrogen receptor nucleic acid expression. This technique involves cleavage by means of ribozymes containing nucleotide sequences complementary to one or more regions in the mRNA that attenuate the ability of the mRNA to be translated. Possible regions include coding regions and particularly coding regions corresponding to the catalytic and other functional activities of the estrogen receptor proteins of the present invention, such as ligand binding.

The nucleic acid molecules also provide vectors for gene therapy in patients containing cells that are aberrant in estrogen receptor gene expression. Thus, recombinant cells, which include the patient's cells that have been engineered ex vivo and returned to the patient, are introduced into an individual where the cells produce the desired estrogen receptor protein to treat the individual.

The invention also encompasses kits for detecting the presence of an estrogen receptor nucleic acid in a biological sample. For example, the kit can comprise reagents such as a labeled or labelable nucleic acid or agent capable of detecting estrogen receptor nucleic acid in a biological sample; means for determining the amount of estrogen receptor nucleic acid in the sample; and means for comparing the amount of estrogen receptor nucleic acid in the sample with a standard. The compound or agent can be packaged in a suitable container. The kit can further comprise instructions for using the kit to detect estrogen receptor protein mRNA or DNA.

Design of SNP-Containing Nucleic Acids Detection Methods

The SNP-containing nucleic acid molecules of the present invention are useful as probes, primers, chemical intermediates, and in biological assays for SNPs of the present invention. The probes/primers can correspond to one or more of the SNPs provided in FIGS. 1-3 , 8 and 9 or can correspond to a specific region 5′ and/or 3′ to a SNP position. However, as discussed above, fragments are not to be construed as encompassing fragments that are not associated with SNPs of the present invention or those known in the art for SNP detection. The SNP-containing nucleic acid molecules and information provided herein are also useful for designing primers for PCR to amplify any given SNP of the present invention and to design any formatted SNP detection reagent/kits.

A probe/primer typically comprises substantially a purified oligonucleotide or oligonucleotide pair. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 20, 25, 40, 50 or more consecutive nucleotides. Depending on the particular application, the consecutive nucleotides can either include the target SNP position, or be a specific region in close enough proximity 5′ and/or 3′ to the SNP position to carry out the desired assay.

Preferred primer and probe sequences can readily be determined using the sequences provided in FIGS. 1-3 , 8 and 9 . It will be apparent to one of skill in the art that such primers and probes are useful as diagnostic probes or amplification primers for genotyping SNPs of the present invention, and can be incorporated into a kit format.

For analyzing SNPs, it may be appropriate to use oligonucleotides specific to alternative SNP alleles (referred to as “allele-specific oligonucleotides”, “allele-specific probes”, or “allele-specific primers”). The design and use of allele-specific probes for analyzing polymorphisms is described by e.g., Saiki et al., Nature 324, 163-166 (1986); Dattagupta, EP 235,726, Saiki, WO 89/11548.

In a hybridization-based assay, allele-specific probes can be designed that hybridize to a segment of target DNA from one individual but do not hybridize to the corresponding segment from another individual due to the presence of different polymorphic forms in the respective segments from the two individuals. Hybridization conditions should be sufficiently stringent that there is a significant difference in hybridization intensity between alleles, and preferably an essentially binary response, whereby a probe hybridizes to only one of the alleles. Some probes are designed to hybridize to a segment of target DNA such that the polymorphic site aligns with a central position (e.g., in a 15-mer at the 7 position; in a 16-mer, at either the 8 or 9 position) of the probe. This design of probe achieves good discrimination in hybridization between different allelic forms.

Allele-specific probes are often used in pairs, the “pairs” may be identical except for a one nucleotide mismatch that represents the allelic variants at the SNP position. One member of a pair perfectly matches a reference form of a target sequence and the other member perfectly matches a variant form. In the case of an array, several pairs of probes can then be immobilized on the same support for simultaneous analysis of multiple polymorphisms within the same target sequence.

In one type of PCR-based assay, an allele-specific primer hybridizes to a site on target DNA overlapping the SNP position and only primes amplification of an allelic form to which the primer exhibits perfect complementarity. See Gibbs, Nucleic Acid Res. 17 2427-2448 (1989). This primer is used in conjunction with a second primer that hybridizes at a distal site. Amplification proceeds from the two-primers, resulting in a detectable product that indicates the particular allelic form is present. A control is usually performed with a second pair of primers, one of which shows a single base mismatch at the polymorphic site and the other of which exhibits perfect complementarity to a distal site. The single-base mismatch prevents amplification and no detectable product is formed. The method works best when the mismatch is included in the 3′-most position of the oligonucleotide aligned with the polymorphism because this position is most destabilizing to elongation from the primer (see, e.g., WO 93/22456). This PCR-based assay can be utilized as part of the TaqMan assay, described below.

SNP Detection Kits, Nucleic Acid Arrays, and Integrated Systems

The present invention further provides SNP detection kits, such as arrays or microarrays of nucleic acid molecules, or probe/primer sets, that are based on the SNPs provided in FIGS. 1-3 , 8 , 9 .

In one embodiment of the present invention, kits are provided which contain the necessary reagents to carry out one or more assays that detect one or more SNPs disclosed herein. The present invention also provides multicomponent integrated systems for analyzing the SNPs provided by the present invention.

SNP detection kits may contain one or more oligonucleotide probes, or pairs of probes, that hybridize at or near each SNP position. Multiple pairs of allele-specific oligonucleotides may be included in the kit to simultaneously assay large numbers of SNPs, at least one of which is one of the SNPs of the present invention. In some kits, such as arrays, the allele-specific oligonucleotides are provided immobilized to a substrate. For example, the same substrate can comprise allele-specific oligonucleotide probes for detecting at least 1; 10; 100; 1000; 10,000; 100,000; 300,000 or substantially all of the polymorphisms shown in FIGS. 1-3 , 8 and 9 .

Specifically, the invention provides a compartmentalized kit to receive, in close confinement, one or more containers which comprises: (a) a first container comprising one of the nucleic acid probes, for example an allele-specific oligonucleotide, that can bind to a fragment of the human genome containing a SNP disclosed herein; and (b) one or more other containers comprising one or more of the following: wash reagents or reagents capable of detecting the presence of a bound probe.

In detail, a compartmentalized kit includes any kit in which reagents are contained in separate containers. Such containers include small glass containers, plastic containers, strips of plastic, glass or paper, or arraying material such as silica. Such containers allow one to efficiently transfer reagents from one compartment to another compartment such that the samples and reagents are not cross-contaminated, and the agents or solutions of each container can be added in a quantitative fashion from one compartment to another. Such containers may include a container which will accept the test sample, a container which contains the SNP probe, containers which contain wash reagents (such as phosphate buffered saline, Tris-buffers, etc.), and containers which contain the reagents used to detect the bound probe. The kit can further comprise reagents for PCR or other enzymatic reactions, and instructions for using the kit. One skilled in the art will readily recognize that the previously unidentified SNPs of the present invention can be routinely identified using the sequence information disclosed herein and can be readily incorporated into one of the established kit formats which are well known in the art.

The present invention further provides arrays or microarrays of nucleic acid molecules that are based on the sequence information provided in FIGS. 1 and 3 , including one or more of the variations provided in FIG. 2 .

As used herein “Arrays” or “Microarrays” refers to an array of distinct polynucleotides or oligonucleotides synthesized on a substrate, such as paper, nylon or other type of membrane, filter, chip, glass slide, or any other suitable solid support. In one embodiment, the microarray is prepared and used according to the methods described in U.S. Pat. No. 5,837,832, Chee et al., PCT application WO95/11995 (Chee et al.), Lockhart, D. J. et al. (1996; Nat. Biotech. 14: 1675-1680) and Schena, M. et al. (1996; Proc. Natl. Acad. Sci. 93: 10614-10619), all of which are incorporated herein in their entirety by reference. In other embodiments, such arrays are produced by the methods described by Brown et al., U.S. Pat. No. 5,807,522. Arrays or microarrays are commonly referred to as “DNA chips”.

Any number of oligonucleotide probes, such as allele-specific oligonucleotides, may be implemented in an array, wherein each probe or pair of probes corresponds to a different SNP position. The oligonucleotides are synthesized at designated areas on a substrate using a light-directed chemical process. The substrate may be paper, nylon or other type of membrane, filter, chip, glass slide or any other suitable solid support.

Hybridization assays based on oligonucleotide arrays rely on the differences in hybridization stability of short oligonucleotides probes to perfectly matched and mismatched target sequence variants. Efficient access to polymorphism information is obtained through a basic structure comprising high-density arrays of oligonucleotide probes attached to a solid support (e.g., a chip) at selected positions. Each DNA chip can contain thousands to millions of individual synthetic DNA probes arranged in a grid-like pattern and miniaturized to the size of a dime, each corresponding to a particular SNP position or allelic variant. Preferably, probes are attached to a solid support in an ordered, addressable array.

The array/chip technology has already been applied with success in numerous cases. For example, the screening of mutations has been undertaken in the BRCA 1 gene, in S. cerevisiae mutant strains, and in the protease gene of HIV-1 virus (Hacia et al., 1996; Shoemaker et al., 1996; Kozal et al., 1996). Chips of various formats for use in detecting SNPs can be produced on a customized basis.

An array-based tiling strategy useful for detecting SNPs is described in EP 785280. Briefly, arrays may generally be “tiled” for a large number of specific polymorphisms. “Tiling” refers to the synthesis of a defined set of oligonucleotide probes that are made up of a sequence complementary to the target sequence of interest, as well as preselected variations of that sequence, e.g., substitution of one or more given positions with one or more members of the basis set of monomers, i.e. nucleotides. Tiling strategies are further described in PCT application No. WO 95/11995. In a particular aspect, arrays are tiled for a number of specific SNPs. In particular, the array is tiled to include a number of detection blocks, each detection block being specific for a specific SNP or a set of SNPs. For example, a detection block may be tiled to include a number of probes that span the sequence segment that includes a specific SNP. To ensure probes that are complementary to each allele, the probes are synthesized in pairs differing at the SNP position. In addition to the probes differing at the SNP position, monosubstituted probes are also generally tiled within the detection block. Such methods can readily be applied to the SNP information disclosed herein.

These monosubstituted probes have bases at and up to a certain number of bases in either direction from the polymorphism, substituted with the remaining nucleotides (selected from A, T, G, C and U). Typically the probes in a tiled detection block will include substitutions of the sequence positions up to and including those that are 5 bases away from the SNP. The monosubstituted probes provide internal controls for the tiled array, to distinguish actual hybridization from artefactual cross-hybridization. Upon completion of hybridization with the target sequence and washing of the array, the array is scanned to determine the position on the array to which the target sequence hybridizes. The hybridization data from the scanned array is then analyzed to identify which allele or alleles of the SNP are present in the sample. Hybridization and scanning may be carried out as described in PCT application No. WO 92/10092 and WO 95/11995 and U.S. Pat. No. 5,424,186.

Thus, in some embodiments, the chips may comprise an array of nucleic acid sequences of fragments of about 15 nucleotides in length. In further embodiments, the chip may comprise an array including at least one of the sequences selected from the group consisting of those disclosed in the FIGS. 1-3 , 8 and 9 , and the sequences complementary thereto, or a fragment thereof, said fragment comprising at least about 8 consecutive nucleotides, preferably 10, 15, 20, more preferably 25, 30, 40, 47, or 50 consecutive nucleotides and containing a polymorphic base. In some embodiments the polymorphic base is within 5, 4, 3, 2, or 1 nucleotides from the center of the polynucleotide, more preferably at the center of said polynucleotide. In other embodiments, the chip may comprise an array containing any number of polynucleotides of the present invention.

An oligonucleotide may be synthesized on the surface of the substrate by using a chemical coupling procedure and an ink jet application apparatus, as described in PCT application WO95/251116 (Baldeschweiler et al.) which is incorporated herein in its entirety by reference. In another aspect, a “gridded” array analogous to a dot (or slot) blot may be used to arrange and link cDNA fragments or oligonucleotides to the surface of a substrate using a vacuum system, thermal, UV, mechanical or chemical bonding procedures. An array, such as those described above, may be produced by hand or by using available devices (slot blot or dot blot apparatus), materials (any suitable solid support), and machines (including robotic instruments), and may contain 8, 24, 96, 384, 1536, 6144 or more oligonucleotides, or any other number which lends itself to the efficient use of commercially available instrumentation.

Using such arrays, the present invention provides methods of identifying the SNPs of the present invention in a sample. Such methods comprise incubating a test sample with an array comprising one or more oligonucleotide probes corresponding to at least one SNP position of the present invention, and assaying for binding of a nucleic acid from the test sample with one or more of the oligonucleotide probes. Such assays will typically involve arrays comprising oligonucleotides probes corresponding to many SNP positions and/or allelic variants of those SNP positions, at least one of which is a SNP of the present invention.

Conditions for incubating a nucleic acid molecule with a test sample vary. Incubation conditions depend on the format employed in the assay, the detection methods employed, and the type and nature of the nucleic acid molecule used in the assay. One skilled in the art will recognize that any one of the commonly available hybridization, amplification or array assay formats can readily be adapted to employ the novel SNPs disclosed herein. Examples of such assays can be found in Chard, T, An Introduction to Radioimmunoassay and Related Techniques, Elsevier Science Publishers, Amsterdam, The Netherlands (1.986); Bullock, G. R. et al., Techniques in Immunocytochemistry, Academic Press, Orlando, Fla. Vol. 1 (I 982), Vol. 2 (1983), Vol. 3 (1985); Tijssen, P., Practice and Theory of Enzyme Immunoassays: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1985).

The test samples of the present invention include, but are not limited to, nucleic acid extracts, cells, and protein or membrane extracts from cells, which may be obtained from any bodily fluids (such as blood, urine, saliva, phlegm, gastric juices, etc.), cultured cells, biopsies, or other tissue preparations. The test sample used in the above-described methods will vary based on the assay format, nature of the detection method and the tissues, cells or extracts used as the sample to be assayed. Methods of preparing nucleic acid, protein, or cell extracts are well known in the art and can be readily be adapted in order to obtain a sample that is compatible with the system utilized.

Multicomponent integrated systems may also be used to analyze SNPs. Such systems miniaturize and compartmentalize processes such as PCR and capillary electrophoresis reactions in a single functional device. An example of such technique is disclosed in U.S. Pat. No. 5,589,136, which describes the integration of PCR amplification and capillary electrophoresis in chips.

Integrated systems can be envisaged mainly when microfluidic systems are used. These systems comprise a pattern of microchannels designed onto a glass, silicon, quartz, or plastic wafer included on a microchip. The movements of the samples are controlled by electric, electroosmotic or hydrostatic forces applied across different areas of the microchip to create functional microscopic valves and pumps with no moving parts. Varying the voltage controls the liquid flow at intersections between the micro-machined channels and changes the liquid flow rate for pumping across different sections of the microchip.

For genotyping SNPs, the microfluidic system may integrate, for example, nucleic acid amplification, minisequencing primer extension, capillary electrophoresis, and a detection method such as laser induced fluorescence detection.

In a first step, the DNA samples are amplified, preferably by PCR. Then, the amplification products are subjected to automated minisequencing reactions using ddNTPs (specific fluorescence for each ddNTP) and the appropriate oligonucleotide minisequencing primers which hybridize just upstream of the targeted polymorphic base. Once the extension at the 3′ end is completed, the primers are separated from the unincorporated fluorescent ddNTPs by capillary electrophoresis. The separation medium used in capillary electrophoresis can be, for example, polyacrylamide, polyethyleneglycol or dextran. The incorporated ddNTPs in the single nucleotide primer extension products are identified by laser-induced fluorescence detection. This microchip can be used to process at least 96 to 384 samples, or more, in parallel.

Vectors/Host Cells

The invention also provides vectors containing the nucleic acid molecules described herein. The term “vector” refers to a vehicle, preferably a nucleic acid molecule, that can transport the nucleic acid molecules. When the vector is a nucleic acid molecule, the nucleic acid molecules are covalently linked to the vector nucleic acid. With this aspect of the invention, the vector includes a plasmid, single or double stranded phage, a single or double stranded RNA or DNA viral vector, or artificial chromosome, such as a BAC, PAC, YAC, OR MAC.

A vector can be maintained in the host cell as an extrachromosomal element where it replicates and produces additional copies of the nucleic acid molecules. Alternatively, the vector may integrate into the host cell genome and produce additional copies of the nucleic acid molecules when the host cell replicates.

The invention provides vectors for the maintenance (cloning vectors) or vectors for expression (expression vectors) of the nucleic acid molecules. The vectors can function in procaryotic or eukaryotic cells or in both (shuttle vectors).

Expression vectors contain cis-acting regulatory regions that are operably linked in the vector to the nucleic acid molecules such that transcription of the nucleic acid molecules is allowed in a host cell. The nucleic acid molecules can be introduced into the host cell with a separate nucleic acid molecule capable of affecting transcription. Thus, the second nucleic acid molecule may provide a trans-acting factor interacting with the cis-regulatory control region to allow transcription of the nucleic acid molecules from the vector. Alternatively, a trans-acting factor may be supplied by the host cell. Finally, a trans-acting factor can be produced from the vector itself. It is understood, however, that in some embodiments, transcription and/or translation of the nucleic acid molecules can occur in a cell-free system.

The regulatory sequence to which the nucleic acid molecules described herein can be operably linked include promoters for directing mRNA transcription. These include, but are not limited to, the left promoter from bacteriophage λ, the lac, TRP, and TAC promoters from E. coli , the early and late promoters from SV40, the CMV immediate early promoter, the adenovirus early and late promoters, and retrovirus long-terminal repeats.

In addition to control regions that promote transcription, expression vectors may also include regions that modulate transcription, such as repressor binding sites and enhancers. Examples include the SV40 enhancer, the cytomegalovirus immediate early enhancer, polyoma enhancer, adenovirus enhancers, and retrovirus LTR enhancers.

In addition to containing sites for transcription initiation and control, expression vectors can also contain sequences necessary for transcription termination and, in the transcribed region a ribosome binding site for translation. Other regulatory control elements for expression include initiation and termination codons as well as polyadenylation signals. The person of ordinary skill in the art would be aware of the numerous regulatory sequences that are useful in expression vectors. Such regulatory sequences are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual. 2nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., (1989).

A variety of expression vectors can be used to express a nucleic acid molecule. Such vectors include chromosomal, episomal, and virus-derived vectors, for example vectors derived from bacterial plasmids, from bacteriophage, from yeast episomes, from yeast chromosomal elements, including yeast artificial chromosomes, from viruses such as baculoviruses, papovaviruses such as SV40, Vaccinia viruses, adenoviruses, poxviruses, pseudorabies viruses, and retroviruses. Vectors may also be derived from combinations of these sources such as those derived from plasmid and bacteriophage genetic elements, eg. cosmids and phagemids. Appropriate cloning and expression vectors for prokaryotic and eukaryotic hosts are described in Sambrook et al., Molecular Cloning A Laboratory Manual. 2nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., (I 989).

The regulatory sequence may provide constitutive expression in one or more host cells (i.e. tissue specific) or may provide for inducible expression in one or more cell types such as by temperature, nutrient additive, or exogenous factor such as a hormone or other ligand. A variety of vectors providing for constitutive and inducible expression in prokaryotic and eukaryotic hosts are well known to those of ordinary skill in the art.

The nucleic acid molecules can be inserted into the vector nucleic acid by well-known methodology. Generally, the DNA sequence that will ultimately be expressed is joined to an expression vector by cleaving the DNA sequence and the expression vector with one or more restriction enzymes and then ligating the fragments together. Procedures for restriction enzyme digestion and ligation are well known to those of ordinary skill in the art.

The vector containing the appropriate nucleic acid molecule can be introduced into an appropriate host cell for propagation or expression using well-known techniques. Bacterial cells include, but are not limited to, E coli , Streptomyces, and Salmonella typhimurium . Eukaryotic cells include, but are not limited to, yeast, insect cells such as Drosophila, animal cells such as COS and CHO cells, and plant cells.

As described herein, it may be desirable to express the peptide as a fusion protein. Accordingly, the invention provides fusion vectors that allow for the production of the peptides. Fusion vectors can increase the expression of a recombinant protein, increase the solubility of the recombinant protein, and aid in the purification of the protein by acting for example as a ligand for affinity purification. A proteolytic cleavage site may be introduced at the junction of the fusion moiety so that the desired peptide can ultimately be separated from the fusion moiety. Proteolytic enzymes include, but are not limited to, factor Xa, thrombin, and enterokinase. Typical fusion expression vectors include pGEX (Smith et al., Gene 67:31-40 (1988)), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann et al., Gene 69:301-315 (1988)) and pET 11d (Studier et al., Gene Expression Technology: Methods in Enzymology 185:60-89 (1990)).

Recombinant protein expression can be maximized in a host bacteria by providing a genetic background wherein the host cell has an impaired capacity to proteolytically cleave the recombinant protein. (Gottesman, S., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990)119-128). Alternatively, the sequence of the nucleic acid molecule of interest can be altered to provide preferential codon usage for a specific host cell, for example E coli . (Wada et al., Nucleic Acids Res. 20:2111-2118 (1992)).

The nucleic acid molecules can also be expressed by expression vectors that are operative in yeast. Examples of vectors for expression in yeast e.g., S. cerevisiae include pYepSec1 (Baldari, et al., EMBO J. 6:229-234 (1987)), pMFa (Kurjan et al., Cell 30:933-943(1982)), pJRY88 (Schultz et al., Gene 54:113-123 (1987)), and pYES2 (Invitrogen Corporation, San Diego, Calif.).

The nucleic acid molecules can also be expressed in insect cells using, for example, baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., Sf 9 cells) include the pAc series (Smith et al., Mol. Cell Biol. 3:2156-2165 (1983)) and the pVL series (Lucklow et a!, Virology 170:31-39 (1989)).

In certain embodiments of the invention, the nucleic acid molecules described herein are expressed in mammalian cells using mammalian expression vectors. Examples of mammalian expression vectors include pCDM8 (Seed, B. Nature 329:840(1987)) and pMT2PC (Kaufman et al., EMBO J. 6:187-195 (1987)).

The expression vectors listed herein are provided by way of example only of the well-known vectors available to those of ordinary skill in the art that would be useful to express the nucleic acid molecules. The person of ordinary skill in the art would be aware of other vectors suitable for maintenance propagation or expression of the nucleic acid molecules described herein. These are found for example in Sambrook, J., Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989.

The invention also encompasses vectors in which the nucleic acid sequences described herein are cloned into the vector in reverse orientation, but operably linked to a regulatory sequence that pemits transcription of antisense RNA. Thus, an antisense transcript can be produced to all, or to a portion, of the nucleic acid molecule sequences described herein, including both coding and non-coding regions. Expression of this antisense RNA is subject to each of the parameters described above in relation to expression of the sense RNA (regulatory sequences, constitutive or inducible expression, tissue-specific expression).

The invention also relates to recombinant host cells containing the vectors described herein. Host cells therefore include prokaryotic cells, lower eukaryotic cells such as yeast, other eukaryotic cells such as insect cells, and higher eukaryotic cells such as mammalian cells.

The recombinant host cells are prepared by introducing the vector constructs described herein into the cells by techniques readily available to the person of ordinary skill in the art. These include, but are not limited to, calcium phosphate transfection, DEAE-dextran-mediated transfection, cationic lipid-mediated transfection, electroporation, transduction, infection, lipofection, and other techniques such as those found in Sambrook, et al. ( Molecular Cloning: A Laboratory Manual, 2nd, ed, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989).

Host cells can contain more than one vector. Thus, different nucleotide sequences can be introduced on different vectors of the same cell. Similarly, the nucleic acid molecules can be introduced either alone or with other nucleic acid molecules that are not related to the nucleic acid molecules such as those providing trans-acting factors for expression vectors. When more than one vector is introduced into a cell, the vectors can be introduced independently, co-introduced or joined to the nucleic acid molecule vector.

In the case of bacteriophage and viral vectors, these can be introduced into cells as packaged or encapsulated virus by standard procedures for infection and transduction. Viral vectors can be replication-competent or replication-defective. In the case in which viral replication is defective, replication will occur in host cells providing functions that complement the defects.

Vectors generally include selectable markers that enable the selection of the subpopulation of cells that contain the recombinant vector constructs. The marker can be contained in the same vector that contains the nucleic acid molecules described herein or may be on a separate vector. Markers include tetracycline or ampicillin-resistance genes for prokaryotic host cells and dihydrofolate reductase or neomycin resistance for eukaryotic host cells. However, any marker that provides selection for a phenotypic trait will be effective.

While the mature proteins can be produced in bacteria, yeast, mammalian cells, and other cells under the control of the appropriate regulatory sequences, cell-free transcription and translation systems can also be used to produce these proteins using RNA derived from the DNA constructs described herein.

Where secretion of the peptide is desired, which is difficult to achieve with multi-transmembrane domain containing proteins such as estrogen receptors, appropriate secretion signals are incorporated into the vector. The signal sequence can be endogenous to the peptides or heterologous to these peptides.

Where the peptide is not secreted into the medium, which is typically the case with estrogen receptors, the protein can be isolated from the host cell by standard disruption procedures, including freeze thaw, sonication, mechanical disruption, use of lysing agents and the like. The peptide can then be recovered and purified by well-known purification methods including ammonium sulfate precipitation, acid extraction, anion or cationic exchange chromatography, phosphocellulose chromatography, hydrophobic-interaction chromatography, affinity chromatography, hydroxylapatite chromatography, lectin chromatography, or high performance liquid chromatography.

It is also understood that depending upon the host cell in recombinant production of the peptides described herein, the peptides can have various glycosylation patterns, depending upon the cell, or maybe non-glycosylated as when produced in bacteria. In addition, the peptides may include an initial modified methionine in some cases as a result of a host-mediated process.

Uses of Vectors and Host Cells

The recombinant host cells expressing the peptides described herein have a variety of uses. First, the cells are useful for producing a estrogen receptor protein or peptide that can be further purified to produce desired amounts of estrogen receptor protein or fragments. Thus, host cells containing expression vectors are useful for peptide production.

Host cells are also useful for conducting cell-based assays involving the estrogen receptor protein or estrogen receptor protein fragments, such as those described above as well as other formats known in the art. Thus, a recombinant host cell expressing a native estrogen receptor protein is useful for assaying compounds that stimulate or inhibit estrogen receptor protein function.

Host cells are also useful for identifying estrogen receptor protein mutants in which these functions are affected. If the mutants naturally occur and give rise to a pathology, host cells containing the mutations are useful to assay compounds that have a desired effect on the mutant estrogen receptor protein (for example, stimulating or inhibiting function) which may not be indicated by their effect on the native estrogen receptor protein.

Genetically engineered host cells can be further used to produce non-human transgenic animals. A transgenic animal is preferably a mammal, for example a rodent, such as a rat or mouse, in which one or more of the cells of the animal include a transgene. A transgene is exogenous DNA which is integrated into the genome of a cell from which a transgenic animal develops and which remains in the genome of the mature animal in one or more cell types or tissues of the transgenic animal. These animals are useful for studying the function of a estrogen receptor protein and identifying and evaluating modulators of estrogen receptor protein activity. Other examples of transgenic animals include non-human primates, sheep, dogs, cows, goats, chickens, and amphibians.

A transgenic animal can be produced by introducing nucleic acid into the male pronuclei of a fertilized oocyte, e.g., by microinjection, retroviral infection, and allowing the oocyte to develop in a pseudopregnant female foster animal. Any of the estrogen receptor protein nucleotide sequences can be introduced as a transgene into the genome of a non-human animal, such as a mouse.

Any of the regulatory or other sequences useful in expression vectors can form part of the transgenic sequence. This includes intronic sequences and polyadenylation signals, if not already included. A tissue-specific regulatory sequence(s) can be operably linked to the transgene to direct expression of the estrogen receptor protein to particular cells.

Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and are described, for example, in U.S. Pat. Nos. 4,736,866 and 4,870,009, both by Leder et al., U.S. Pat. No. 4,873,191 by Wagner et al. and in Hogan, B., Manipulating the Mouse Embryo , (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986). Similar methods are used for production of other transgenic animals. A transgenic founder animal can be identified based upon the presence of the transgene in its genome and/or expression of transgenic mRNA in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying a transgene can further be bred to other transgenic animals carrying other transgenes. A transgenic animal also includes animals in which the entire animal or tissues in the animal have been produced using the homologously recombinant host cells described herein.

In another embodiment, transgenic non-human animals can be produced which contain selected systems which allow for regulated expression of the transgene. One example of such a system is the cre/loxP recombinase system of bacteriophage P1. For a description of the cre/loxP recombinase system, see, e.g., Lakso et al. PNAS 89:6232-6236 (1992). Another example of a recombinase system is the FLP recombinase system of S. cerevisiae (O'Gorman et al. Science 251:1351-1355 (1991). If a cre/loxP recombinase system is used to regulate expression of the transgene, animals containing transgenes encoding both the Cre recombinase and a selected protein is required. Such animals can be provided through the construction of “double” transgenic animals, e.g., by mating two transgenic animals, one containing a transgene encoding a selected protein and the other containing a transgene encoding a recombinase.

Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut, I. et al. Nature 385:810-813 (1997) and PCT International Publication Nos. WO 97/07668 and WO 97/07669. In brief, a cell, e.g., a somatic cell, from the transgenic animal can be isolated and induced to exit the growth cycle and enter G o phase. The quiescent cell can then be fused, e.g., through the use of electrical pulses, to an enucleated oocyte from an animal of the same species from which the quiescent cell is isolated. The reconstructed oocyte is then cultured such that it develops to morula or blastocyst and then transferred to pseudopregnant female foster animal. The offspring born of this female foster animal will be a clone of the animal from which the cell, e.g., the somatic cell, is isolated.

Transgenic animals containing recombinant cells that express the peptides described herein are useful to conduct the assays described herein in an in vivo context. Accordingly, the various physiological factors that are present in vivo and that could effect ligand binding, estrogen receptor protein activation, and signal transduction, may not be evident from in vitro cell-free or cell-based assays. Accordingly, it is useful to provide non-human transgenic animals to assay in vivo estrogen receptor protein function, including ligand interaction, the effect of specific mutant estrogen receptor protein on estrogen receptor protein function and ligand interaction, and the effect of chimeric estrogen receptor protein. It is also possible to assess the effect of null mutations, that is mutations that substantially or completely eliminate one or more estrogen receptor protein functions.

EXAMPLES:

SNP Identification and Characterization

The method used to identify SNPs in the ESR-beta gene was sequencing of overlapping ESR-beta PCR products amplified by primers listed below in the tables. Using DNA extracted from the blood and tumors of 48 breast cancer (BC) patients and a Coriell Diversity Panel, PCR was performed to amplify exons—7 through 9 of ESR2, plus approximately 100 bases of each flanking intron. PCR products were sequenced. Sequences for exons—7 to −2 were compared with cDNA sequence from AB06589 and an “assembled” BAC AL161756. Sequences for exons—1 through 9 ( FIG. 2 ) were compared to a BAC sequence representing 101 kb of ESR2 (all but the most 5′ and 3′ exons known to us), referred to here as Contig64 (FIG. 1 ), to discover single nucleotide polymorphisms (SNPs). Sequences for exon 10 were compared with an assembled BAC sequence representing all of ER2, referred to as ER2 BAC. PolyPhred version 2.0 (D. A. Nickerson, S. Taylor, N. Kolker, Univ. of Washington, 1998) was run on the sequences (with default settings) to visualize potential heterozygotes. Tagged sites were examined for quality to verify polymorphisms. 21 SNPs were found with a frequency greater than 2, with 4 being unique to the clinical samples, and 4 unique to the Coriell Samples. Six of these SNPs had been observed in ESR2 cDNA, isolated from a pool of prostate mRNA from 47 individuals (Clontech). 15 of the 16 SNPs had at least one instance of a change in heterozygosity, and 5 had at least one instance of a loss in heterozygosity. Of the three samples unique to the clinical samples, one was observed only in tumors.

TABLE 1
Summary of SNPs found in clinical samples with a frequency greater
than 2 and quality score greater than 20.
Total Number of SNPs       21
Number in Liverpool        16
Number in Coriell          17
Number unique to Liverpool 4
Number unique to Coriell   4

TABLE 2
Summary of changes in heterozygosity in clinical samples. SNPs had a
frequency greater than two and a quality score greater than 20.
Number of Liverpool SNPs 15
with >1 Change in Heterozygosity
Number of Liverpool SNPs 5
with >1 case of Loss of Heterozygosity

A summary of the SNPs that were identified is provided in FIG. 2 ( a ). SNPs were observed in 48 breast cancer patients. Genomic DNA was isolated from blood (B; 96 chromosomes) and matched tumor tissue (T; 96 chromosomes).

FIG. 2 ( b ) shows SNPs in pooled Coriell diversity panels. Some of the SNP sites were examined in 5 Coriell human diversity panels. Exons were PCR-amplified from pools of 3 or 4 individuals. Percent of chromosomes carrying the minor allele, calculated from peak height, was reported for each diversity panel, which represented 20 chromosomes (N. Eur, Northern European; Chi, Chinese; In-Pak, Indo-Pakistani; Af-Am, African-American; SW-NA, Southwestern Native American). As a reference, the frequency of these SNP's were observed in Liverpool BC samples was shown.

FIG. 2 ( c ) shows SNPs in unpooled Coriell diversity panels: The SNP sites were examined in 5 Coriell human diversity panels of 10 individuals each.

FIG. 2 ( d ) shows SNPs in CEPH families. SNP's found in clinical samples were validated in a second reference population of 3 CEPH families: Caucasian, African-American (Af-Am), and Asian. Each family represents 4 individuals, or 8 chromosomes. Percent of chromosomes carrying the minor allele (SNP allele) was also shown.

FIGS. 2 ( e ) and ( f ) show the primer set and M13 primers that were used for overlapping PCR and clone sequencing. FIG. 2 ( e ) shows the primers used to generate ix-(intron-exon) PCR products. Exons—7 though 8 were M13 tailed PCR primers. All sequencing done with M13 forward and reverse except for exon 9 in where the PCR primers were used for sequencing. FIG. 2 ( f ) shows primers used for sequencing exon 10.

FIG. 5 shows the domain structure of the ESR-beta protein and the position of many of the SNPs disclosed herein. FIGS. 6 a , 6 b and 7 provide a graphical representation of introns/exons of the ESR-beta gene.

FIG. 8 ( a ) shows the SNPs and frequency of occurance in Coriell Samples wherein the samples are collected from Northern European, Chinese, Indo-Pakistani, Africa American and Southwestern Native American ethnic groups. The result can be used for detection purpose among the specific ethnic groups. FIG. 8 ( b ) shows the SNPs and frequence of occurance in Liverpool samples wherein the samples are selected from the groups of blood samples and breast cancer samples.

2. ESR 2 Genomic Sequencing—The Complete Genomic Structures of Estrogen Receptor Beta

Estrogen receptor (ER) is a member of the nuclear hormone receptor gene superfamily. This family of genes is characterized by a modular structure with three distinct domains: a variable (N)-terminal domain, a highly conserved DNA binding domain, and a conserved (C)-terminal domain (Reviewed in 1, 2). Functionally, the (N)-terminus domain regulates transactivation, the DNA binding domain regulates dimerization and DNA binding, and the (C)-terminus domain regulates transactivation, dimerization, ligand binding, nuclear translocation, silencing, and Heat Shock Protein binding. It was shown that the functions of the individual domains of the nuclear hormone gene superfamily are independent of the receptor in which they are found, and that the domains retain their function even when placed into different heterologous proteins (3,4,5). The domain modularity in the nuclear hormone receptor gene superfamily exists because the major subfamilies of these genes evolved through a simple gene duplication early in evolution (6). The nuclear hormone receptor gene family can be separated according to two different classification schemes, one based on hormone binding, the other based on dimerization and how the receptors bind to their respective DNA response elements (for a review, see 2).

The cDNA for ERα was first cloned and sequenced from the MCF-7 breast cancer cell line and was found to have 27% identity and 41% conservation to the v-erb-A gene (7). ERα was mapped to chromosome 6q25.1 using Fluorescence In Situ Hybridization (FISH) and chromosome banding (8). In 1996, a novel estrogen receptor (ERβ) was identified by degenerate PCR (9) and mapped to 14q22-24 by FISH (10). ERα and ERβ were shown to have 96% sequence identity in the DNA binding domain, 58% identity in the ligand-binding domain, and low similarity in the 5′ and 3′ ends as well as in the hinge (domain D). A variety of ERα and ERβ variants have since been described, including single and multiple exon deletions, truncated transcripts, and transcripts containing insertions (11,12,13). These variants were isolated from a variety of sources, including normal tissues, tumor tissues and cell lines. The ER status of tumors in breast cancer patients has been used as an indicator of response to endocrine therapy (14,15), and many studies have examined the role of ER in breast cancer tumor progression, ER-negative status, and hormone antagonist resistance (for a complete review, see 16).

Because of the importance of the ER gene, we set about to clone it in its entirety and determine its complete structure. Initially, we used standard Bacterial Artificial Chromosome (BAC) sequencing to generate sequence information for the coding regions of the genes. As Celera's sequencing of the human genome progressed, the remaining regions of ER were filled in using Celera regional assemblies. A small region of less than 25 kb was filled in on ERα using a public BAC (A1353611.6, positions 1,497-25,941)

Materials and Methods

1) BAC Screening

Appropriate markers were designed for ERα and ERβ exons and used to obtain commercially available BAC clones from Research Genetics (Huntsville, Ala.). A number of positive BACs were selected and individual clones were re-screened for verification.

2) DNA Isolation and Library Preparation

BAC DNA was isolated from verified clones using QIAGEN columns (QIAGEN, Inc., Valencia, Calif.) according to the manufacturer's specifications. Shotgun libraries were prepared following standard protocols (17). Briefly, isolated BAC DNA was sonicated, polished, and size fractionated. Size selected DNA fragments were then subcloned into pUC19 using standard ligation techniques. Ligated DNA was transformed into Electrocompetent cells (Life Technologies, Rockville, Md.) and grown overnight.

3) DNA Sequencing and Annotation

Sequencing reactions were performed using Big Dye Terminator chemistry (Applied Biosystems, Foster City, Calif.) and run on an ABI PRISM 3700 DNA Analyzer (Applied Biosystems). Phred (18), Phrap and Consed (19) were used for base calling, assembly, and finishing, respectively. Exon locations were determined using Cross_Match to compare the published gene sequences to the genomic contig.

Results

1) Estrogen Receptor α

Alignment of the genomic sequence for ERα and published mRNA sequences for ERα show the gene consists of 14 exons and covers 446,296 bp of genomic sequence.

2) Estrogen Receptor β

Alignment of the genomic sequence for ERβ and published mRNA sequences for ERβ show the gene consists of 17 exons and covers 253,748 bp of genomic sequence ( FIG. 5 , Table 3). By analysis with the Celera Genome Browser, we were able to identify a gene, human synaptic nuclei expressed gene 2 (syne-2, accession number NM — 015180.1), that is completely contained within intron 9 of ERβ, on the opposite strand. Further analysis of the syne-2 gene showed it consists of 21 exons, and covers 51,471 bp of genomic sequence.

Discussion

Alignment of the complete ERα genomic sequence and various ERβ transcripts shows that the gene covers 446,296 bp of genomic sequence and consists of 14 exons. The alignment of the published sequence for exon 1 E (AJ002561) (20) and the ER a genomic sequence revealed that exon 1 E actually consists of two separate exons. The newly delineated exon is referred to here as exon 1 G to conform to the naming convention previously established. Exon 1 G is located approximately 45 kb upstream of exon 1 E and conforms to the GT/AG splice site consensus sequence.

Alignment of the various ERβ transcripts to the complete ERβ genomic sequence reveals a more complex organization than was previously accepted (13). The 5′ UTR of the ERβcx variant (AB006589) actually consists of seven untranslated exons (referred to here as exons −1 through −7), all of which conform to the GT/AG splice site consensus sequence ( FIG. 5 , table 3). Sequence alignment of ERβ variants AF061055 and AF061054 (12) showed that these transcripts both contain intron sequence and were probably partially mature transcripts. Both of these partially mature transcripts contain exon 7 and a portion of exon 9, but do not conform to the splice site consensus sequence at the sites where intron sequence is present.

By examining the ER genomic sequences using the Celera Genome Browser, we were able to identify a separate gene contained entirely within intron 9 of ERβ. This gene was identified as human synaptic nuclei expressed gene 2 (syne-2) and was shown to cover over 50 Kb of genomic sequence and consist of 21 exons, all of which conform to the GT/AG splice site consensus sequence (FIG. 5 and Table 4). The syne-2 gene is located on the antisense strand of ERβ.

Completion of the sequence and structures for ERα and ERβ should contribute to further understanding and characterization of these important receptors.

TABLE 3
Exon-Intron Boundaries and Locations in the Human Estrogen Receptor: Exon
sequences are shown in upper case and intron sequences are shown in lower case. Splice
sites are shown in bold.
								Intron
	Exon	Splice	Contig	Contig			Exon	size
Gene	no.	variant	start	end	5′ splice donor	3′ splice acceptor	Size (bp)	(Kb)
ER1	1G	AJ002561	18941	19032	—	ACCAAAGAAG gt aagttttt	91	33.79
	1F	AJ002562	52818	52940	—	TTCTCTTCAA gt aggtactc	122	11.21
	1E	AJ002561	64150	64280	aaaacaaa ag GAAGAAGAAA	CATCACTGAG gt atgtgtga	130	101.95
	1D	AJ002560	166228	166322	—	GAGAGAGCCA gt aagtcacg	94	1.68
	1C	X62462	168002	168120	—	ATCCAGCAGG gt aggcttgt	118	1.55
	1B	AJ002559	169674	169825	—	GACAAGTAAA gt aaagttca	151	0.04
	1A	X03635	169867	170678	—	CATTCTACAG gt acccgcgc	811	34.23
	2	X03635	204912	205102	ttcccccc ag GCCAAATTCA	AGTATTCAAG gt aatagtgt	190	37.87
	3	X03635	242970	243086	cttttaat ag GACATAACGA	ATGAAAGCTG gt aggtacat	116	63.08
	4	X03635	306168	306503	gtgttttc ag GGATACGAAA	AGGGTGCCAG gt aagaatgc	335	67.14
	5	X03635	373640	373778	ttgttttc ag GCTTTGTGGA	TCTTGGACAG gt aagtgacc	138	49.19
	6	X03635	422964	423097	gttttcat ag GAACCAGGGA	CTTAATTCTG gt gagttgat	133	33.26
	7	X03635	456354	456537	gcgcattc ag GAGTGTACAC	GGCACATGAG gt gaggcatc	183	4.16
	8	X03635	460701	465237	ccacctac ag TAACAAAGGC	—	4536	—
ER2	−7	AB006589	49552	49750	—	GGTTCTGAAG gt gcgtggtt	198	1.18
	−6	AB006589	50928	51235	tgcctctt ag ACATCCAAGT	TGTTTGTAAG gt aataaaaa	307	32.62
	−5	AB006589	83858	84041	tatccact ag AGGGAGACAT	GAGAACACAG gt gaacttca	183	1.90
	−4	AB006589	85942	86154	ctctccat ag AAATCCTGGG	ATTAGCCCTG gt aaggagct	212	2.88
	−3	AB006589	89037	89130	cattcaac ag TATCTGGGCT	GTGCAGGTAG gt aggtaaag	93	0.67
	−2	AB006589	89803	89988	ccttttac ag GGTTTTGTTT	GTGTTGACAG gt aagatgag	185	3.12
	−1	AF080555	93111	93488	—	TATCTGCAAG gt aagcgccc	377	10.96
	1	AF060555	104446	104897	ttctttac ag CCATTATACT	CTGTAAACAG gt aagtccag	451	2.47
	2	AF060555	107368	107540	tgctccct ag AGAGACACTG	AGCATTCAAG gt acaagaga	172	11.07
	3	AF060555	118610	118726	tctgctat ag GACATAATGA	GTGAAGTGTG gt gagtgctt	116	8.05
	4	AF06055	126774	127073	tcctcttc ag GCTCCCGGAG	AAGATTCCCC gt agggcttt	299	3.09
	5	AF060555	130158	130296	ctttcccc ag GCTTTGTGGA	TTCTGGACAG gt gagaaaaa	138	7.56
	6	AF060555	137853	137986	acttttgt ag GGATGAGGGG	CTCAATTCCA gt aagtaatc	133	14.39
	7	AF060555	152379	152559	ctttgtcc ag GTATGTACCC	GGCATGCGAG gt acgcgccc	180	1.65
	8	X99101	154206	154500	gtccccat ag TAACAAGGGC	—	826	5.42
	9	AB006589	159915	160827	tctactta ag GGCAGAAAAG	—	912	141.65
	10	AF060555	302474	303300	gtcttgac ag CTCTCTCTCA	—	826	—

TABLE 4
Exon-Intron Boundaries and Locations in the Human Synaptic Nuclei Expressed
Gene 2. Exon sequences are shown in upper case and intron sequences are shown in
lower case. Splice sites are shown in bold.
							Exon
	Exon	Splice	Contig	Contig			Size	Intron
Gene	no.	variant	start	end	5′ splice donor	3′ splice acceptor	(bp)	size (Kb)
Syne-2	1	NM_015180	212563	212391	—	CACTGTAGAG gt aaactcac	172	2.22
	2	NM_015180	210175	210044	tttcaaat ag ACCTGGGACC	GCTGATTAAG gt attgaaat	131	8.94
	3	NM_015180	201109	200946	ttaaatgc ag GAACTAGAAC	CTGCTTAAGG gt aagtcagc	163	1.97
	4	NM_015180	198981	198819	tcatttgc ag GTGGCCATAC	GTTACAGAAG gt aagggagg	162	1.36
	5	NM_015180	197462	197290	cctttgcc ag GACTGCATGG	TCGGATCAAG gt aagaaatg	172	12.56
	6	NM_015180	184732	184564	atatgtgt ag GGTGAAGAAG	TGAGCAGCAG gt gggacaat	168	5.79
	7	NM_015180	178777	178584	gtaatcac ag GATCTACAGC	GGCGCATGAA gt aagaacta	193	0.48
	8	NM_015180	178101	177949	ctcccatc ag AATCGAGGAG	GAGGTTTGAG gt aaacacct	152	0.36
	9	NM_015180	177591	177405	tgtgatgc ag GCCTTTCAGC	GAGACTCAGG gt gagctcct	186	1.84
	10	NM_015180	175570	175429	acttttgc ag CATTTCACCA	CCAACTGAAT gt gagggctg	141	0.71
	11	NM_015180	174718	174522	ctctcaac ag GGCTTCCAAC	CTGCACTCCG gt acgggcac	196	1.19
	12	NM_015180	173337	173051	tgtggttt ag GGCTTGGAAG	GCACTGTCAG gt aacagctg	286	1.76
	13	NM_015180	171289	171140	ttcgtttc ag GTAAATCCAT	ACCACCCTAT gt aagtctta	149	2.00
	14	NM_015180	169139	169013	ctcattct ag GGAAAGCTAC	CAGCAGTCAG gt actgcctg	126	0.69
	15	NM_015180	168327	168117	ttaattcc ag GTGCCTTCGA	GAGACTGCAG gt gagttaga	210	1.02
	16	NM_015180	167096	166890	tctctggt ag GAGATACTGA	GCAGTGCCAG gt acgctgac	206	0.93
	17	NM_015180	165957	165825	gtttttta ag GACTTCCACC	GGAACTAATG gt aagtttcc	132	1.48
	18	NM_015180	164342	164149	ctgttttc ag CAACTGGAAA	GGGAACCCAG gt gagtctac	193	1.08
	19	NM_015180	163074	162982	tgaatttc ag AACCCAGCCT	CCGAGCAAAG gt aagaagcc	92	0.45
	20	NM_015180	162537	162482	ctttaccc ag CAGTTCAGAG	CAGAGAGCAG gt aacggggc	55	0.27
	21	NM_015180	162214	161092	ctgttggc ag GGTCCCCGGC	—	1122	—

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All publications and patents mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the above-described modes for carrying out the invention which are obvious to those skilled in the field of molecular biology or related fields are intended to be within the scope of the following claims.

3 1 325791 DNA HUMAN 1agcccgctgt ttcaggcccc gccgatctgg aaggagtgtc agagctggag cgcgcgtggc 60ctcatcggtg ttggggtcac cccggggttg ccagggctca tggagggtcg tagtctggat 120tttgtcaccc ccacgtcccc gccccgcagc aagtctgggg ttggagaact cacgcggtct 180tcgtaagcta catgccagtt gaccctcgag gagggatgct ccctcccctt aagcgtccac 240gctggagaag gagtaagatg gacaattgcc tggggagcct gacagggcgg tggcagctgg 300gatgctggag aggactggcc ccttgtgtta ctgagtccaa ggaatatgct tgctctgctc 360taggaaccgc gttcaggtta cagtcatccc agtagagtcc tgaagatgcg tggttcaggt 420cacttaggac ttgaccagat accgggtttc ttttacaagc cgtttactac tggcagagct 480catctaaaac tttttttgtt tgtttgtttg agacggagtc tcattctgtc gcccaggctg 540gagtgcagtg gcacgatttt ggctcactgc aacctccgct tcccgggttc aagcagttct 600ctgcctcagc ctcccgagta gctgggatta caggcaccac ctaattagcc cgccaccacg 660cccggctaat ttttgtattt ttagtagaga cggggtttca ccatcttggc caggctggtc 720ttgaactcct gacctcgtga tccacccgcc tcggcctccc agagtgctgg gattacaggc 780gtgagccacc gcacctggcc taaaactgat tttttattaa ttttggggct tttaatattt 840ttttcttatt tctaaattct gaggttattt atagtagccc catatacggg attagataat 900ctcttgtgat tttctatttc tggtaattat ttctaatata tgtttttttg tttttgagac 960ggagtctcgc tatgtcgctc agggtggagt gcagtggcat gatctccgct ctttgtagcc 1020tctgcctccc gggttcaaat gatcctccca cctcagcccc ccgagtagct tggaccacag 1080gtgcatgcca ccacgccggc taatttttct atttttggta gagttggggt ttcactatgt 1140tgaccagact ggtctagatc tcaagggatt agtctccctt ggcctcccaa aatgctggga 1200taatgggcat gagccaccgt gcgtggcctt aaagttacta ttcttaaagt ttgcacaagt 1260gatatgttaa aggcacagac ttagtaatat aatgtcatta taataataac cctaaaacac 1320attgtctcat attgtgttgt acctaaacaa gtgaaattaa gaagaaaatt gaaggaaatg 1380tttctggtaa attgcagata gtgaatcttt tgtcttatac tatcaaatag gtattgacta 1440ttccagcttt cttatttgtt gaggaagatg gcagaaatcc cattttacag agggatagac 1500tttgaaggat aatacccaaa gctgcatagc tgtggctggt ataggcccca aacctgatgt 1560ttcttctcta aatctactgc ctttgccatc tcaacagcct ggtttttgac agttatctat 1620gtatgagttg cataaatcgt tcattcatgg agcaaataat tattgagtgg ccactatgcc 1680aacagcactg ctatagatgc tagagatacc ctagtgaacc agcaaagttt ctgctctcag 1740ctcatattct ggtggaggag acaacgatca agttaaagaa atacataggc taattttaga 1800gattatgaca tgctatattt taaaaatagg caagctaaga ggataggcag tgatgctggg 1860aggtgggaaa gttttgtctc agaaatgtgg taagagattt ctttgggcat ctgacttcag 1920cagaaacctt aatgaagaga ggaacttgga atgtaaaaga aagaaagcag ggatttgctc 1980tgagcaactg gaaagatgga attgccattc gctgagttga aataaagtaa aatgtaggac 2040taggttttgg ggttaagatt atgaattcgg ctttagacat ttttagattt ctcttagaca 2100tccaaatgga gagaagatat ttaaatccat gggattgaat gagatccaac caagggtatt 2160gtaggtagag agaggaccaa agactgacac ctagaacctt tcagtgttca gaatgcaagg 2220agacaggagg aaccaagagg gaagattgaa aaggagagtc cagctgggag ctgtggctac 2280acctttacta atcccagcac tttgggagac caaatacagg aagatcactt gaatccagga 2340gtttgtagaa cagccttagc aacatagcaa gaccctgtct ctacaaaaat aaaaattaaa 2400aaatttgcct gtaatccgag cattttggag gccgaggtgg gtggatcagc tgaggtcagg 2460agtttgagac caacttggcc aacacagtga aaccccgtct ctactaaaaa tacaaaaatt 2520agctgggcgt gatggctggt acctgtaatc ccagctactc gggaggccga ggcaggagaa 2580tcccttgaac ctaggaggca gaggttgcag tgagccgaga tcacgccact gcactccagc 2640ctgggagaca gagcgagact ccatctcaaa ataaataaat aaataaaaat ttaaaaagtt 2700agctgggcgt ggtggcatgt acctgtagtc ccagttactc agaaggctga ggtgggagga 2760tcctgtgagc ctaggagttg gaaacttcag taagctataa tcatcacact gcactccaac 2820ctaggcaaca gagcaagacc ctgtctctta aaaggaaagg agagtccagt gtgttctaag 2880gaaaacccca agagcatccc accttagaag acaagtgagg aggcctggca tggtgtctca 2940tgcctgtaat cccagcactt tgggaggcca aggtgggtgg atcacttgag gtcaggagtt 3000ctagaccagc ctgacaaaca tggcgaaacc tccgtctcta taaaaataca aaaattagcc 3060aggtgtggta gcgcgtgcct gtaatcccag ctactaggga ggctgaggca ggagaactgc 3120ttgaactcag ggggcagagt ttgcagtgag ccgagatagt gccactgcac tccagcctga 3180gcaacagagt gagactctgt ctcaaaaaca aacaagtaaa caaacaaaca aaaaaaacaa 3240gacaagtgaa gaatgtgttt catggaaaaa gaggtaatta attctgtcaa gtgttgcaaa 3300ttggtcaaat aaagaatgaa aatcaacctt tcacagcaaa tagaaggaaa aaatattttt 3360atttaaatgc ttataaaggc agttgctaga aaaaatgttt actttttgca gaggccccgt 3420ttttacaacc tttttcaggg gtaatttgat atgataatat ctacgggaaa aaaaatgttt 3480tttttttgag tcgctctgtc accaggctgg agtgcagtgg catgatctcg gctcactgaa 3540acctccgcct cccaggttca agcaattctt ctgcctcagc ctcccaagta gctgggacta 3600caggtgggcg ccaccacacc cagctaattt ttgtattttt agtagagacg gggtttcacc 3660atgttggcca ggatggtctc aatcttgacc tcgtgatctg ccttccttgg cctcccaaag 3720tgctgggatt ggtacaggtg tgaaccacca tacctggccc gaaaatttta aatttgtatt 3780ttctttgaca ttgtaattcc acttccagga tttttttttt ttggacagga ttttactctg 3840tcacccaggc tggagtgcag taacacaatc agctcactgc aaccctgaac tcctgggcta 3900aagtgatcct tctgagtagt tgggactata ggcacatgcc accacccctg actaattaaa 3960aaattttcct gtagagatag tcttgctatg ttgcccagac tggtctccaa ctcctggcct 4020caaaccatcc tcccaccttg accttccaaa acgttgggat tacaggcgtg agccacctct 4080cccagtttag gaatttatct taaagtaata tttacatata aagaaagatg tataggctgg 4140gtgcagtggt tcacgcctgt aatcccagca ctttggaagg ccgaggctgg tggatcaact 4200gaggtcagga gttcaagacc aacctggcta acatggcgaa accccatctc tactaaaaat 4260acaaaaatta gctgggcgtg gtggccagcc cctgtaatcc cagctactcg ggaggctgag 4320gcaggaggat agcttaaacc caggagacga aggttgcagt gagccaagat tacaccattg 4380cactccagcc tgagcaacaa aagcgaaact ccatctcaaa aaaacaaaaa ctaaaactaa 4440acaaaacaaa aaaaccatgt gtaaaactgt taatcacaac actgatttca atagcaaaaa 4500acaaaatttc tgtcaggatg gatgcagttg ttaaaggaac agcagataca tatgtataga 4560catgtaagat agctttcata cttttttttt tgagatggaa tttcgttctt gtcacccagg 4620ctggaatgca atggtgagat ctaggctccg cttctcaggt tcaagtgatt ctcctgcctc 4680agcctcctga gtaagctggg aatacaggcg cccgccacca cgcccaggta atttttgtat 4740gtttagtaga gacagggttt cgccatattg gcgaggcttg tctcaaactc ctgacctcag 4800gtgatccacc cacctccaaa agtgttggga ttacaggcat cacccaccac gcgtgaccag 4860cttttataca tttttaaatg ataaagacag gttaataaaa tgtataatat tatgttgcta 4920tatccaaaaa aaggccttct ttgattacac tatcaaaagt tacctctcca tttacatccc 4980cattactatc tcattaacct gttttattca tagcacttac taccatctaa aatgacttta 5040tttctttata tttttgtttg ctacctgtct tttacaccag aatttaattt ttaagctttt 5100tttgtttgtt tgtttgtttg ttttgagatg gagtcttgct ctgttgccca gactggagtg 5160cagtggtgcg atctcagctc actgcaacct ctgcctcctg ggttcaatca atcctcctgc 5220ctcagcctcc caagtagctg ggattacagg catgtgccac catgcccagc taatttttgc 5280atttttagta cagcagggtg tcaccatgtt ggtcaggctg gtctcaaact cctgacctca 5340ggagatccgt cagccttggc ctcccaaagt gttgggatta caggcgtgag ccacagcacc 5400cagccagaat ttaaacttta taagagattt cctgtcttgt tcatacttat atacctgcag 5460cttcgaaaca tatatatatg tttaatatat ataatagata tattttaaat tatatataga 5520gagatggagt ttcactcttg ttgcccagac tggagtgcaa tggcgtgatc tcggctcacc 5580acaacctctg ccccccgggt tcaagcaatt ctcctgcctc agcctccaga gtagttggaa 5640ttacaggcac gtgccaccat gcctggctaa ttttgtattt ttagtagaga cagagtttct 5700ccatgttggt caggctggtc tcgagctctt gacctcaggt agtccgcctg cctcggcctc 5760acaaagtgct gggattacaa gcatgagtca ctgtgccctg ccagaattta agctttataa 5820gagatttcct gtctgtttat acttaaatac ctgcagcttg gaaacatata tatgtttata 5880tgtatatatt acatacatta tatatatatt tttatatatt ataattataa actcctgacc 5940tcaggtgatc cacctgcctc agtctctcaa atgctgggat tacaggcgtg agccaccacg 6000ccaggccaga aacatatttt taaaaatctt gttttagagg aaaaacagag tatttctttt 6060ttttttttat tagatggatt ctcactctat tgcccaggct ggagtacagt ggcacaatct 6120tggctcaccg caacctcagc ctcccgggtt caagcaattc tcctgcctca gcctcccgag 6180tagttggaac tataggcgtg agccaccatg ccctgctaat ttttgtattt ttagtacaga 6240tggggtttca ctatgttggc caggctggtc tcgaactcct gaccttgtga tctgcccacc 6300tcggcctccc aaagtgctgg gattacaggt gtgagccact gcgcccggcc tatttcttct 6360tctctttgtt tagttattat ctattactct cattcctatg aacataactt gtttctcccc 6420ccttaatttt tatcatacat gattgtagac agtgggcact gtcttcaatt atagtgaatt 6480tagcagtaaa ttcacattag accaacttgt atagctttaa aaaatatatt tatgtctagg 6540ttccaccctt gaccaactaa gtcagaactt gggtgggttc aaggttcatt attctttgaa 6600gataagatga tgtttgaata aaattcctgg tgattctggt atcaaaaata caaatttggg 6660acatactttt tctgctgtaa aaatattttc ctaaggccag gcgcagtggc tcacgcctgt 6720aatcctagca ctttgggaga cggaggcggc agatcacttg aggccaggag ttcaagacca 6780gtctggccaa catggtgaaa cccagtctct actaaaaata gaaaaaatta gccaggcatg 6840gtggcacgtg cctgtagtcc cagctactca agaggctgag gcaggagaat cctttgaacc 6900cgggaggcag aggttgcagt gagctgatat tgcagcactg cactccagcc tgggtgacag 6960atcaaaactc tgtctcagaa aaaaaaaaaa aaaaaaaaga atttcctaga attagaatcg 7020caggggtttt ttttgttttg tttgtttgtt tgtttgtttg tttttgagac agagtttcac 7080tcctgtcgcc caggctggag tgcaatgcca tgatctcggc tcattgcaac ctctgcctcc 7140tgagttcaag caatcctcct gcctcagcct cccgaatagc tgggattaca ggcacctgcc 7200accatgccca gctaattttt gtatttttag tagagactgg gttttaccat gttggccaag 7260ctggtctcga actcctgacg tcaggtgatc cacccagctc attctcccaa agtgctggaa 7320ttacaagcat gagccactgc actcggcctt tatttattta tttatttttg agatgaagtc 7380ttgctctgtt gcccaagctg gagtgcaatg gcatgatctc ggctcactgc aacctccacc 7440tcccaggttc aagcaattct cctgcctcag ccccctgagt agctgggatt acaggcgtgc 7500accaccacgc ctggctaatt tttgtatttt tagtagagac agggtttcac tatgttggtc 7560aggttagtct cgagctcctg acttcgtgat ccgcccgcct cagccttcca aagtgttggg 7620attacaggcg tgagccaccg cgcctggcca gaatcccagt tttttaacac atctaatgct 7680ttaggaatag taaatggaaa catcatttcc ccttctttcg aagtacttct accttgatga 7740gatgtatgta ttggagtaca atatttgacc tagccaagaa tttcacaaaa gaagcccaaa 7800atatgatttt cacgtttact ggactgttca cttttggggg gatcactttc ttaagattac 7860ttaaagtact aatgcttgat gaaaatcatt tgtgttttca cttcattaat tggagaaaga 7920gctacatgtt tttgttgttg ttgttgttgt tttgagatgg tgtcttgctc tgtctcccag 7980gctggagtgc aatggcgtga tctcagctca ctgcaacctc cgcctcccgg gttcaagcga 8040ttctcctgcc tcagcctcct gagtagctgg gactacaggc gcctgccacc acgcccagct 8100aatttttata cttttaatag agacagggtt tcaccatatt ggccaggatg gtctcaatct 8160cttgaccttg tgatccaccc gccttggcct cccaaagtgc tgggattaca ggcgtgagcc 8220actgcgccca gccagagcta caggttttac tgtaacactt tagaaggtcc tttttctttg 8280tacctctgtg atgtgttcac ctagcaacag cttctttgat atgcaaacat tctacaggca 8340aattgctcag agcagctact catgttggac aattcaggtc tcttctggaa actggccttg 8400tatttggaat tttctccaga gtctgatgtg gtaaaaattt atacttttca cttcttacta 8460aatgcagcat agcatactac atcttttgag tgtgtaaaaa ataaagtagt catgtacaaa 8520cctaaaatca cagaataatc atgtaatggt gctatatttt atttttactt tagattccta 8580atacattttt attgtatctt tataaggtta gtttgggaga atagcactat catcatctat 8640attagttaac tttgcattaa ggtttttcat taaggttttt cccctgtttg gttctttcat 8700tttacgatcc atattttttg tgcctggtat gggatgataa agataacaga aatagctcct 8760gtttttaggt acctttattt gtttgtttgt ttggagacag ggtcttactc tgtcacccag 8820gctggtggca caatcatagc ttactacaga ctaactcctg gctcaagcca tcctccacct 8880cagcctccca agtaactgag attacaggtg cacaccacca cctctggcta attattaaaa 8940ttttcataga gacaggatct cacttggttg gccagactga tctcaaactc ctggtctcaa 9000gtgatcctcc caccccagcc tcctgaagta ctaggattgc aggcatgaac cactgccctg 9060gcctagatac atttaatgta gtagaggaga tgagattttt acatagtata atattgaatg 9120agacaacagt caaaagaaaa tcacatacta taagagaaga gattaccctt accttttagg 9180aatcaagaaa actccctgga ggagatggta cttgaactta tactggagga tttatatgtt 9240catccttctt ggtttatatt ttgtcccaca cggtagcttg tttctttttt cttttttttc 9300tttttctttt tttttttttt gaggcagagt tttgctctgt tgcccaggct agagtgcagt 9360ggcgcgatct cggctcactg caacctccgc ctcctgggtt caagcgattc tcctgcctca 9420gcctccctag tagcttgttt cttagagaca gtgtgagtga aactcattaa gtaaagtcaa 9480tacagcacaa gtttcataaa atggtaaaga aatagaaata aaagtaaagg atgaaattct 9540taagaacttt gtcaggccgg gcgtggtggc tcatgcctgt aatcccagca ctttgggagg 9600ccgaggtggg tggatcacct gaggtcagga gtttgagacc agcctggcca acatggcaaa 9660accctgtctc tctaaaaata caaaaattag ctgggcatgc tggcgggtgc ctataatccc 9720agctactcgg gaggctgagg caggagaatc gcttgaaccc aggaggcgga ggttgcagtg 9780agccgagatc acatcattcc actccagcct gggtgccaag agcaaaaaac tccatcttga 9840aaaataaaga attttgtctc cagtatcatt cttgttctag gactgagaaa cagttcattc 9900ttcattttcg tctactaaaa tttaagttct ttgattttct tctttaggtc ttggaaaaag 9960aattaataga gtaattttct taggtatcaa gtaatgaaaa tgagaaaaga gactagtctc 10020attatagatt tttttttttt attcttattt tattttattt gagacaaggt ctcgctctgt 10080cacccaggct ggagtgtagt ggctccatta tggttccctg cagccttgaa ctcaggggcc 10140caagcaatcc tcccacctca gcatcccgag cagctgggac tacaggtgca tgccaccatg 10200cctggctaat ttttgtgctt tttcgtgtat aaagatgaga tttcgccatg ttgcccaggc 10260tggtctcaaa ctcctgggct caagagatct gtccacctca gcctcctaaa gcgctgagat 10320tacaggcatg agccaccacg tccagcctag agatatttat actttaatag ctgcctgcaa 10380taccaataca cctagaatga attagaagaa tttgaaaaca gatttcagga ttagtaaaag 10440aaagtcaagc cctatgcctc ctgttctaca ttcctcaaat cacataatcc tgattttttc 10500ctcttcgtat gaaaattcct ctcattttgg gcctgtttga ttttttggga gacaccaccc 10560agcagaaaca caccgcactt acatgcaacc cctccattgc acagtttcta aatgcccccc 10620cccttttttt tccattctct gatcctaaaa aaagaaatcc agtggcacaa tcacggatct 10680ctgcagcctt gatctcttag cctcaagtga ttctcctgcc tcagcttcct aagtagctgg 10740gaccacaggt gcttgccacc atgcccagct attttttttt ctttgttttt ttgtaaagat 10800gtgtctcact atcttgccca ggctgatatt gaattcctgg gctcaagtga tcctcctacc 10860ttggcctccc aaagtgctgg gattttatat atatatatat ttccatttta cttatgttaa 10920tgctttccct cttttggcat agaaaagcta ataaagctta aacttaaatg gaacttgtat 10980aaacacaata tatctgatga tacctttgag agatgtctta catttgtctt ttctttcagc 11040aaaatgtgat gccagtccct gaaatgtgta gcaatttaat tatgggtagc attcttcttt 11100ccctgtactt actatagtat taacttacta ctaatgctaa gaagtttata atttttgata 11160attcagattt ggattagatt aagatttatg tctatgcata attcattaaa aactttttaa 11220accaaattgt caaaaaagat tgtaggtacc ttgtttaaag aaaatatata agctagtttc 11280aagaattcca aaatattttt taaaagcagc tctgtacatg tcgataaatt atttgctcat 11340tgtaattttt tgaatctgtt tgtcaaagca aatgtagacg ggctcgaact cctgacctca 11400aatgatccac ccgcctcagt ctcccaaatg ctggtattac aggcatgagc caccgctcat 11460ggcctgttat catttttaat tgaaaatttt actgagataa ttgtagattc acttgccatt 11520ataagaaata attcagagat atcacttgta tacttagccc agtgtccccc aaaggtaaaa 11580ttttgcaaaa ttatagtcta atgtaacagc gtgaatattg acattaatac aatccactga 11640gtttattcag atttccccag ttttacttgt attcaattgt gtgtttgtgt attaagttcg 11700atataactag tcaatatact gaacagttct aacatcacaa gtatccttca ggtagccctt 11760ttgtatccac atccacttcc ttctcatccc cagctattga caaccactaa tcccttttcc 11820atttctaaaa tgtgatttca aaaatgttac atacttggct gggggcagtg gctcatgcct 11880gtaatcccag cactttggat atactgtctc tactaaaaat acaaaaatta gctgggagtg 11940gttgtgcaca cctgtaatcc caggtgcttg ggaagctgag gcaggagaat tgcttgaacc 12000caggaggcag agcttgcagt gagccgagag tgtgccactt cactccagcc tgggcaacag 12060agtgagactc tgtttcaaaa ataaataaat aaataaataa aatggtcttc atacagcctt 12120tgctgctagg aaatatagtt tctaattaat gtaatttttt gtcaaataca aaatgcttct 12180gaacgtttct ggttatcaag ctggtaatct ttcacagtgt cctcaatttt tttttctact 12240ctcttactag ttctccagat cacatgcagg ctattaagaa atgtgaacta acaagttaaa 12300gtagcaggac gggcgctgtg gctcacacct ataatcccag cactttggga agctgaggca 12360ggtggatcac ttgaggtcag gagtttgaga gcagcctggc caacaaggtg aaaccacatc 12420tctactaaaa aaaaaaaaaa tttgccaggt gtggtggtgc atacctgtaa tcctagctat 12480ttgggaggct gaggcatgag aatcgcttga acccaggagg cagaggctgc ggggagccag 12540gatcatagca ctgcactcca gcctgggtga cagagctaga ccctgtctga aaaaaaaaaa 12600aaatgttagt agcagcagag cacagtggct catgcctgta atcccaacac tatgggagtc 12660tgaggcagga ggatctcttg agcttaggag ttcaagacca gcctgacaac atagtaagac 12720tccatctcta caaataatca ttaaaaaaat tagccgggcg tagtggcacc tacctgtagt 12780cccagctact ggggaggctg agatgggagg atcacctgag cctgggaggt caaggctgca 12840gtgagctgtg atcgccattg tactccaaac tgggtgacaa gagtgagacc atgcctcaaa 12900aataataata ataaatgtat aatttaaatg tgacctaaca ttatgaagtt tttaaaaaca 12960aaattataaa tgattttaac actttctcat tagctaagaa atcttcagaa caaacttttc 13020ataaaagaaa cttcactgca agagttgaag cctgagctag aaagttacaa agaaaataat 13080gtacgacagt cgttccagat aatgtccctg aaagataata tcaaggacct acagaaactt 13140actgcttctc taaccagaat taaatatttg agaaacacca atattcagag gcttcaaaga 13200ggcaactgga atttaactaa atgaattatt gagctagaaa ctgtctaagg tagggatttt 13260tccagtttgt tttcaaacat gtcttttgtt gtaagcttgc aaatagcata tgaactccat 13320actcctgatt gatcatagaa tttaaatctg cagaatttca cttaatacct gacccaacat 13380tattatattt tgtattgaga tataacttaa atacctcggg ccaggtgtgg tggctcatgc 13440ctataatccc agcactttgg gaggccaggg ctggtgaatc acttgaggtc aggagttcaa 13500aaccagcctg gccaacatga caaaaccctg tctctactaa aaatacaaaa aattagctgg 13560gcatggtggc atgcacctgt aatcccagct actcaggagg ctgaggtaga agaatcactt 13620gaatccagga ggtgcgagac tctgtctaaa aaaaaaaaat ccaatttgag atataactta 13680catacctcaa gattcagtta aagtgtacaa ttcaatggtt gtacaatcat caccactatc 13740taatttcaga acaatttcat cacacctccc caccccccac tgcaaaaaaa aacccatgcc 13800cggctgggca cggtggctca tgcctgagat cccagcactt tgggaggccg agacaggcgg 13860atcacaaggt caggagttcg agaccagcct ggccaacatg gcgaaacccc atctctacta 13920actacaggca ggtgcctgta atcccagcta ctcaggaggc tgaggcagga gaatagctta 13980aatccaggag gctgaggttg tggtgagctg agatcatgcc attgcactcc agcctgagca 14040gcaagagtga aactctgtct caaaaaaaaa aaaaaaaaaa aaaaaagatt taatgattga 14100cctgctggat tttgaacttg catggggcct atagcctctt tcttttggcc aaattctccc 14160ttttggaatg agagtattta cccaatgcct gcaattccca ttatatttcg gaagtcacta 14220acttgttttg ttgttgttgt tgttgttgtt gtttgagaca gagtcttgct ctgttgccca 14280ggctggagtg caatggcaca atctcggctc actgcaacct ctgcctcccg ggttcaagtg 14340attctcctgc ctcggcctcc caagtagctg agatcacagg catgcaccac cacccccggc 14400taatttttgt atttttagca gagatggggg tttcaccatg ttggccaggc tagtctccaa 14460ctcctgactt caggtgatcc accggccttg gcctcccaaa gtgctgggat tacaggcatg 14520agccaccgtg cccagcccga agttactaac ttgttttgga ttttacgggc tcacaggcag 14580aagggacttg ccttgtttca gatgagactt tgttctttgg gcttttgagt taatgctgga 14640atgagttaga actttggggg aactgttggg aaggcagatt gtattttgaa atgtgagaag 14700gacatgagat ttgagagggc ccagtgggag aatgggataa ggcttgagtc tgtgtccctg 14760cccaaatctg atgtcaaagt ataatcccca gtgttagggc ctggtgggag ctgattggat 14820catgggggtg tatttcccct ttggtgctgt tctcatgata gtgagtgagt taccatgaga 14880tctggttgtt taaaagtgtg tagcacctct cacctcactc tattccttct gctctggcca 14940tgtaagatgt gcctgcttcc ccctcacctt ctgccatgat tgtaagtttc ctgaggcttc 15000cctagccatg cttcccatgc agcctgtgga actgtgagcc aattaaacct cttttctttg 15060taaattacct agtctgaagc atttctttac agaagtgcaa gaacagacta atacattgaa 15120catctcttca tgtgcttatt ggccatgtgt atatcttctt tgtagaaata cctattcata 15180tttgttgtcc cttttaaaat tgggttgtct ttttattgct gagttgtaag tgttctttat 15240attttctgga tactggactt ttattaagtg tataatttgt aaatattttc tcccaatttg 15300tgggtcatct ttccactttc ctaaaagtgt catttcaagc aaaaatttta attttgatgg 15360agttgtgtgt gtgtgtgtgt gtgtgtgtat gtgtatgtgt gtctttggtg tcatagctga 15420gaaattattg tcaaatccag gatcatgaaa gatttacatc tatattttct tttaagagtt 15480atagttttgg ccgggcgtgg tggctcatgc ctgtaattcc agcactttgg gaggccaagg 15540caggtggagt tcgagaccag cctggccaac atggtgaaac tccgtcccta ctaaaaatac 15600aaaaattagc tgagcatggt ggcacacgcc tgtaatccca gctgctcgtg aggctgaggc 15660agaagaatag cttgagcccg ggagacagag gttgcagtgg gccaaaatca tgccacggca 15720ctccagcctg gccgacagac tctgtctcaa aaaaaaaaaa agatttatag ttttggctgg 15780gcgtggtggc tcatgcctat aatcccagca tttggggagg ccaaggcagg tggataactt 15840taggccagga gtttgagacc agccgggctg acataacaaa acctgatctc tactaaaagt 15900acaaaactta ggctgggcac agtgactcat gcctgtaatc ccagcactct gggaggccga 15960gatgggcaga taatttgagg ccaggcattg gagaccagcc tggccaacat ggtaaaaccc 16020tgtctctact aaaaatacaa aaatcagctg ggcgtggtgg cacgcacctg taatttcagc 16080tactcgggag gctgaggcgg gagaattgct tgaacccagg aggcagaggt ggcagtgaat 16140tgagatcatg ccactacact tcagcccggg tgatagaacg agactctgtc tcaaaataat 16200aataataata ataataatag ctggacatgg tggtgcacac ctgtagtccc agctacgtgg 16260aaggctgaag caggagaatt gcttgaaccc agaaggtgga ggttgcagtg agccaagatt 16320gtgccactgc actccagcct gggtgacaga gcaagacagg tgtactccag actgggtgac 16380tgcaccccag cctgggtgac actgcactcc agcctgggtg acagagcaac taactaacta 16440actaactaaa actacagtta gtttatatat agtttttagt taaaactata gttagtttga 16500gccaggcgcg gtggctcacg cctgtaatcc cagcactttg ggaggccgag gcgggcggat 16560cacgaggtca ggagatcgag accatcctgg ctaacaaggt gaaaccccgt ctctactaaa 16620aaatacaaaa aattaggcgg gcgtggtgtg gtcccagcta gtcgggaggc tgaggcagga 16680gaatcccttg aacccgggag gcggggcttg cagtgagccg agatcgcacc aatgcacctc 16740cagcctgggc ggcagagcga gactccgtct caaaaaaaaa acaaaaacaa aaacaaaaaa 16800aaagccatag tctttctaaa actatagtta gtttataatt aactatagtt ttagttataa 16860aatataacta taaagttata gttttagcac ttacatttat gtctttgact tcttttggca 16920aatttttatg tatgatgtga ggtaggagtc cagattcatt gtttttcata taaatatcca 16980gttgtcctta gcacctctgt ggaactatct tggcattctt gccaagaatc aattgaccat 17040aaatgtatgg gtttatcttt gggcacccaa ttctatttca ttggtctgta tgtctgtcct 17100tataccagca ccacactgtc ttgattaatg tagctttgta gtaagttttg aaatgggtaa 17160gtgtgaaaaa ttccaactta atttcatttt tttcaagatc attctggcta ttttgggtcc 17220cttgcttttc catatgtatt ttaagatcag cttttccatg aacatggaat atttttccat 17280ttatttaagt cttctttaat ttcttttttt tttttccccg agatggcgtc atgctctgtc 17340gtccaggctg gagtgcagtg gcacgatctc ggctcactgc aacctctgcc tcctgggttc 17400aagcaattct cctgcctcag cctcctgaat tgctaggatt acagatgctc accaccatgc 17460ccagctaatt tttgtatttt tagtagagac agggtttcac catgttagcc agactggtct 17520tgaactcctg accttgtgat ccacccgcct tggcctccca aagtgctggg attacaggcg 17580tgagccaccg cgcctggcct tctttttctt tttcttagag acagggtctc actctgttac 17640ccaggctgca gtgcagtggc acaatcatag ttcagtgtaa tcttgaattc ctgggctcaa 17700gcaatcctcc tgcctcagct tcccaagtag ataggactac agatgcatgc ctccatgctt 17760ggctaatttt taattttttt tttatataga tttggggtct tgctgtgtta cccaggctgg 17820tctcaaactc ctggcctcaa tcaatcttac tgccttggcc ttccaaagca ctgagattac 17880aggcatgcac caccacaccc agcctcttta aattgtttta acaatgtttt gtagttttca 17940gtgtatgtgt gttacatttc ttttgttaaa tttattacta atattttatt ccttttatgc 18000attgtaaatg aaactgtttc catatttcat tttttgattg tttattttta gagagtagaa 18060atacaattga ttcgtgtata tcagtctttg tcctgcaagc ttgctgaact cacttattag 18120ctctagggtt tttttggtat gtgtgtgtgg tttccttggg attttctcca tacaagacta 18180tgaatctgca aatatgtggg tttttaaaaa tttactatta ttattatttt tgagatggag 18240tcatactctg tcatccaggc tggagtgcag tggcacgatc acagctcaca acaacttctg 18300cctcccaggc tcaagcaatt cccctgcctc agcctcctgt agctgagatt acaggagtga 18360accaccatgc ctgactaatt tttgcatttt tagtagagat ggggttttgc catgttgcct 18420aggcttgtct caaactcctg ggttcaggct acccacttgc cttggcctcc caaagtgctg 18480gaattatagg cgtgagccac cacacccaaa tattgttgag tgtttttaat cacaaaagtg 18540tgttggattt tttgtccaat gctttttttt ttttgcatct tactgaaata atcatgtgat 18600tttatcctct atagaatttg ttattgttag acttattgta ttagttggta tacagagaga 18660ggagatattt gataatgtgc cctcagttga caggagaaac aaagtcatta atttttcctc 18720ttataataag aataatattt gagaaactca cacaatatga aaagctattc tattcagatg 18780tgcacactac tgtagcctat ttctattttg tattggttag tatgcatcag gtcatctcat 18840aggttggggc tcttctgagt cttgcatttc tcaaaacact tttttttctt ttgctgtatt 18900tataccaagt cactttttgt ttgtttgttg gttggttggt tggttggttg gttggttggt 18960tggttggttt gagtcggaat ctcactctgt cgccgaggct ggagtgcagt agagtgatct 19020tggctcactg caacctctgc ctcccaggtt caagcgattc tcctgcctca gcctcccaag 19080tagctgggac gacaggtgca cgccactctg cccagctaat ttttgtattt ttagtagaga 19140tggggtttca ccatgttggc caggctggtc tcgaactcct gacctcaggc aagccaccca 19200cctcagactt tcaaagtgct gggattacag gcgtaaacca ctgcgcccag actacttttt 19260aaaagaaatt atataggaga ctaaaattga aaaaagaaac aaacctttac tcagattgag 19320atattagtta aacttaaggg cctgaaacaa ggaaaatggg gtttgctttt ttctggttgt 19380gcagagagtg tatgaataaa aagatctcac aaagttcaag tgaaagactg attaaaagaa 19440attcatcatc caaatatctt ctcagtgtta agcaagcaca tgaagttagc tatagctcga 19500cccttaacag ctaatcaggt aaactcttca actcagtttt gaacgtaaca tagtatacta 19560cagacttttt gtttttgtcc tcagaggtaa agagaaacaa tggctatatg gcatactatg 19620aggattaatt ttatatgtct acttgactgg gccatagggt gcccaatata tggtcaaaca 19680ttattttagg tgtttctgtg agagtgtttt ggataatttt aacatttaaa ttggtatact 19740gagtaaagca gatgatactc cctattgtga gtaggcctca tccactgagt taaaggcctg 19800aatagaacaa aaagattaac cctcccccag gtaagagcga attcttcctg cctgatggcc 19860ttcaaaatgg gacatcagct ctttttcctg cctttggact caaaccattg gctcttcctg 19920ggtcttgagc ctgctggcct ttggactgga gctacactat cagctctctt gattttcagg 19980ccttcaaact tagactcaaa ctacattatt ggctcccctg ggtctcccag atcccagcag 20040atcttgggaa ttgccagcct tcataattgc tgaggcagtt cctttttttt gagatgggat 20100cttcctctgt cacccacact ggagtgtagt ggtacgatca tggcttactg caacctcaaa 20160cacctgggca taagtgatgc tcctgactca gcctcctgag tagcagggac cacaggcaca 20220tgccaccatg cccagctaat tttttaaaaa ttttttgtag atacaaggtc tcactttgtt 20280gcccaggctg gtcttgaact gctgggctca agctatcctt ccacctcagc ctcccaaggt 20340gctgggatta taggcatgag ccactgtgcc cagccaaaag tcagaagata attgaatggc 20400atcttaaagt gctgaaagaa aaaatactga caacccagaa tctatactca gtgaaattat 20460ccttcaaaat tgaagatgag gccgggcgcg gtggctcacg cctgtaatcc tagcactttg 20520ggaggccgag gcgggcggat cacgaggtca ggagatcgag atcatcctgg ctaacatggt 20580gaaaccccgt cagtactaaa aatacaaaaa aaattagccg ggcatggtaa cgggtgcctg 20640tagtcccagc tactcgggag gctgaggcgg gagaatggcg tgaacccagg aggcggagct 20700tgcagtgagc cgagatcgcg ccactgcact ccagcctggg cgagagtgag actccgtctc 20760aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aattgaagat gaaataaaca tggtttcaga 20820agaaaaaaaa agaaatcatt tattgtcaga agaactacac tattaagaaa cacaccaggc 20880aatttttcag gatgaaggaa aatgatcctt ggtggctaca gagaaatgta gggtagaata 20940aggagtaaag aaagggtaaa tagaaattaa tactgacaat taatttttaa aaatcctata 21000caatttataa tatatgaaag agtaaaacat atcagtagta caagggcaag aaatggtaaa 21060tggagttaag ctatcataag tgttttattt agggagtatc acaaaagtat taaagtagaa 21120tgtaataggc cgggcggggt agctcacacc tgtaatccga gtaccttggg aggctaagga 21180gggcggatca cttgaggtca agagttcgag atcagcctgg ccaacatggt gaaaccccac 21240ctctactaaa aatacaaaaa ttagccaggt gtggtggcac acgtctgtaa tcccagctac 21300ttgggaggct gaagcatgag aatagcttgc acgtgggagg cggaggttgc agtgagccga 21360gatcctgtca ctgcactcca gcctgggcga caaagcgaga ctctcaaaaa aaaaaaaaaa 21420aaagcatgta ataaatcaaa tatgcattat gtaatcacaa aatccagctt aacaactaaa 21480taataattta aaagatatta aaaacttaat atggaagaaa aatgaaataa aaaactttat 21540taatccaagg ccgggcgcgg tggctcatgc ctgtaatccc agcactttgg gaggccgagg 21600cgggtggacc acttgaggcc aggagttcca gaccagcctg gccaacatga tgaaaccccg 21660tctctactaa aaatacctgg gcaaagtaga gcatgcctgg ccgggcgcag tggctcatgc 21720ctgtaatccc agcactttgg gaggctgaga cgggtggatc aggaggtcag gagatcgaga 21780ccatcctggc caacatggtg aaaccctgtc tctactaaaa atacaagaat taactgggca 21840tggtggcaca tgcctgtaat tccagctact ctggaagctg aggcaggaga attgcttgaa 21900ccagggaatc aggttgcagt gagctgagat catgccactg cattgcaatc tggcgacaga 21960gcgagattct gtctcaaaaa aaaaaaaaaa aaaaaattag agcatgccta taatctgagc 22020tactcaggag gctgagacac gagaatcact tgaacccagg gcggcggagg tgcagtgagc 22080agcgatcaca ccactgcatt ccagcctggg cgacagagtg agactccctc tcaaaaaaca 22140aacaaacaaa caaacaaaca aacaaacaaa aactttatta atccaaatta aaatggtaga 22200gtagtagtaa aggaacagag aatagatggt aaaaacaata aataacatga taaacccgac 22260aatttcaata ataactttaa agatagatgg tattgtaact gcccaatggg ttcaccttgc 22320ccgctgccta gacagagacg atttctcaag acaggggaat tgcaatagag aaagataaat 22380tcacgcagag ctggctgtat gggagaccag agttttatta ttactcaaat cagtatccac 22440aagcattccg ccttcagaat ttttaaggac aacatgttgg gtgggaggaa gccagtgagc 22500tgggagtgct gattggtcag agctgaaatc atagggaatg gaagctgtct tcttaagctg 22560agtcaattcc tgggtgggga ctgcaagatc agatgagtca ggttatcaat ctacgtggtg 22620ccagctgacc catcaaatgc agggtctgca aaatatctca agcactgatc ttaggagcag 22680tttagggagg gtcagaatct tgtagcctcc agctgcaaga ctcctaaacc ataatttcta 22740atcttgtggt taatgttagt cctaccaagg caatctagtc tccaggcaag aaggaggtct 22800gctttgggaa agggctgtca tctttgtttt aaactataaa ctataaagta agtttctccc 22860aaagttagtt cagcctacac ccaggaatgc acaaggacag tttggaggtt agaaacaaga 22920tggggtcagt taagttagat ctctttcact gtctcaggca taattttgca caggcggttt 22980cagtataatc atttcagtac aagacaagat ttaaaaaaag aaaactaatg ctaagcattt 23040ttcaagaaac atactgtagg ccgggtgcag tggctcacgc ctgtaattcc agcaccttgg 23100gaggctgagg cgggcggatc acgaggtcag gagatcgaga ccatcctggc taacatggtg 23160aaaccccgtc tctactaaaa acacaaaaaa attagccggg tgtggtggcg ggcacctgta 23220gtcccagcta ctcaggaggc tgaggcagga gaatgggtca acccaggagg cagagcttgc 23280agtgagccaa gatcctgcca ctgcactcca gcctgggtga cagagcgaga ctctgtctca 23340aataaaaaaa aagaaggaaa catactgtag ccagacgcca cctatagtcc cagatacttg 23400ggaggctgag gcaggaggat agcttgggac cagtttgaga ccagcctgag caacatagca 23460agaccctgtc tccaaaattt aaaaatgttt aaaaagagat atattttaca tataagaaca 23520cagaaagatg ttattaaaaa taaaaaaatg acatttgggt aatgtgaata ttaccctaaa 23580gaaagctgca tctatgtgga actgttaccg ggaatgggtc ccaatctaga ccccaagaga 23640gggttcttgg acctcacgca agaaagaatt aggggcaaat ccataaagtg aaagcaagtt 23700tattgggaaa gtaaaggaat aaagaatggc tactccatag gtagagtcac agtatgggct 23760gcttaactga gtatactcag ttatttcttg attatatgct aaacaagggg tggattattc 23820atcagttttc tggaaaaggg gcaggcattt ctcggaactg agggttcctt ctttttttag 23880actgtatagg gtaacttcct gatgttgcca tggtatttat aaactgtcat ggccctagtg 23940ggagagtctt ttagcatgct aatgcattat aattagtgta taatgagcac tgaggacaac 24000cagaggtcac ctttgtcacc atcttggttt tggtaggttt gggctatctt ctttatcgca 24060ttctgtttca tcagcagggt ctttgtggtc tgtatcttgt gctgacctcc tatctcatcc 24120tgtgactaag aatgcctaag ctcctgggaa tgcagccagt agttctgagc ttactttacc 24180cagcccctat tcaagatgga gttgctctgg ttctaatgcc tctgacagaa cctcctgatt 24240gtcacaatat taccagtagc agcaggcagc tattcacacc caaatttcac cttaaaaaga 24300atggcttcaa aattgacctc ccccatggga aatattgcag gacttaacac aggattgttt 24360ttctcttcat ttctctacga tttctttctt tctttttttt tccttttttc cgaggtggag 24420tttctctctt tttgcccagg ctggagtgca ctggtgcaat cttggctcac cacaacctct 24480gtctcccggg ttcaagcaat tctcctgcct cagcctcccg agtagctggg attacaggca 24540tgcgccacca cgccaggcta attttgtatt tttagtagag acggtgtttc tccatgttgc 24600aacctcaggt gacccgccca cctcggcctc ccaaagtgct gggattacag gcgtgagcca 24660ctgcacccag cctaccattt ctttttggga cagggtcttg ctgtgttgcc caggctggtt 24720tctaactcct gggctcaagc gatccactgg cctcagcctc ctgaagtgct gggattacag 24780gagtcagcca ctgcaccagg cccatttctc taccatttct gatctctctc tagaaacact 24840tggataactg cacagctcct ttcataagaa atatatttta gggccgggtg tggtggctta 24900tgcctgtaat ctcagcactt tgggaggccg cggcaggtgg atcaaccgag gtcaggagtt 24960caagaccagc ctggccaaga tggtgaaacc ccatctccac taaaaataca aaaaattagt 25020caggagtggt ggtgcatgcc tgtaatccca gctactcagg aggctgaggc aggagaatca 25080cctgaaccca ggaggcggag gttgcagtga cccaacattg cgccattgca tgccagtctg 25140agcaacaaga gtgaaacttt gtctcaaaaa acaaataaac aaatgaacaa acaaacaaaa 25200aatcattgtt attcctcaga gtaagagcaa agatcatccc ctgcagaagc ttaggaacta 25260tgcacagaac tttacagaac aggggcgatg ctttaactga aggctgacta ctgaccagag 25320aatggaattc tgagagggct caaggaataa aaggaaacta ggcagggaaa gggaaggcgc 25380ccatctgaag caaacttcag cggccatcag gatatcttgt ggtggtcaca agttgtaggc 25440tctgtttttg gaaggtttgg gtatagcgca ggattccatt tgtctacttg gctacacctc 25500tgcctgaggt acactgttgc cagaaaagag ggtcccaatc cagaccccaa gagcaggtta 25560ctggatcttg cacaggaaat aattcaaggg aagtcacaca gcacagagaa aaagcaagtt 25620catacaatgt tactgagtaa gttatcgcca gaaagcagga ggaggaacac gccatccttg 25680ttagtgtctc tatttataag aaacttatga gaagctataa ttaaacttgg aacatgcaga 25740tgtgctcact aaaggtaggg gctattggtg ttatagatga ccattaatct ttcaacctaa 25800gcctgctcat taatggcatc tttaataaag tgggctacac tcttaggaca tctggacatt 25860ctgcaggatt ggtgggagat gttctgtatg gccaccaata ttctgtaatt ataattggtg 25920gtcagcttgg gatgtggcta ttttcagacc acaagcatta accttacaga gtgcctagct 25980actcatttca aggtggagtc actctggcca tgttttacca aaccagaggt ctggtaagga 26040gaggttcctc taacaccatg aggtcaacgt cactcaagtt cctggccagc caaactctga 26100aagcaaggag tcccaaattg aggatcaagt tcctccagaa atcattgcta tgggtggcaa 26160agcagtagat tccctgaagc caatctccca cattgtctgg aattttggga gcttcatttg 26220cccagttggc agtgccaatg gggaccccgc tggaagccat agtagcacct ttgggctctg 26280cataggactt gctcaataca ccatattgga gtcgttggct ctttttgtta ttttaagttg 26340aaatttgaag attggctgag ttggtttcat gatattgttg cctactgata tcttacacag 26400agtacgagta catctggttg aaagagaaaa ggcagagaga aaagcagact ttttggagtt 26460gttgttctca ggcactaaca gattcatccc ttatatgcat atgaatggac aataagattt 26520cttggatatt ttcatggcaa aggtgaggag aaatttttat tgctttttga agaaacatga 26580tttttattac cttgggttat tccaggagat aattgataaa tgttgagtag tttgttggtc 26640ttcttcttaa aggaggatca agagaagcag tacagcatgg cagactagaa gcatttctgt 26700ttttttgttt tgttttgttt gagatgaagt ctcactctgt tgccgaggct ggagtgcagt 26760ggagtgatct ctgctcactg caagctccgc ctcctgggtt caagtgattc tcctgcctca 26820gcctccaaag tatctgggat tacaggcatg tgccaccacg cccggctaat ttttacattt 26880ctagtagaga cggggtttca ccatgttagc caggctggtc tcaaactcct gaccttaaat 26940gatccaccca cctcggcctc ccaaagtgct gggattacag gcatgagcca ccgcacctgg 27000ctgaagggtc ttcagtatca tctctgtgat agataaattg tttagtgggt tatctacatt 27060ggccattgac atctagaatt agggtatgag ttgggtttag aagaaggaaa tgaggctggt 27120ctccaataga cagtgattca tatgataagc agaaagtgtt acagaagtct ggtagaggag 27180gatgggagac agattattgt caaggagata gtaaaagttt attttgaaat ccttagacta 27240tgccgggctc agtgggtcac acctgtaatc ccagcacttt gggaggccga ggcgggcgga 27300tcacctgagg tcaggagttc gagaccagcc tgaccaacat gaagaaactc ggtctttatt 27360aaaaatacaa aattagccag gcgtggtggt gcatgcctgt aatcccagct acttgggagg 27420ctgaggcagg agaatcactt gaatctggga ggcggaggtt gtggtgagcc aagatcacgc 27480cattgcactc ctgcctgggc aataagggcg aaactccatc tcaaaaaaaa gaaagaaatc 27540cttagactat aagtttgtta tataatctgc atgtaaaaca aggtcctata tgacttaaat 27600aatgtgcagg gttcccttta aaaagttcag caactacttt cctttttttt tttttgagat 27660ggagtctcac tctgtcaccc aggctggagt gggttggtgc aatcttggct cactgaaacc 27720tctgcctcct gggctcaagc catcttctca cctcagcatc ctcggtaatt tagttgggac 27780tactggcgtg cgccaccatg cccggctaat tttcctgtat tttgtggaga gacggggttt 27840caccatgttg cccaggcttg tctcaaactc ctgggctcaa gtgatcctcc tgccttgatc 27900tcccaaagtg ctaggattac aggcgtgagc caccacacct ggccaatgac tttcaaattt 27960gtttaaagta atcccaatct ttatttgcct ctctcaagta attaatgata acactttctt 28020taaaaaaaaa atgctgtcac taggctgggt gcggtggctc acatctgtaa tcccagcact 28080ttgggaagcc gaggcgggtg gatcacctga ggtcaggagt tcgagaccag cctgaccaac 28140acggtgaaac cctgtctcta ctaaatacaa aaattagcca ggtatggtgg tgggcacctg 28200taattccagc tacttaggag gctgaggaag gagaatcact tgaaccccgg gaggtggagg 28260ttgcattgag ccgagatggc accattgcac tccagcctgg gcaacaagag caaaactgtc 28320tcaaaaaaaa aaaaaaaaaa aaaacaaaaa acagacgggg catggtggct cacacctgta 28380atcccagcac tttgggaggc agaggcaggt ggatcacctg aagtcaggag tttgagacca 28440gcctggccaa catggcaaaa ccccacctct actaaaaata caaaaaaatt agctgggcac 28500ggtggcaggt gcctgtaatc tcatctacta gggaagctga agcaggggaa tcgcttgaac 28560ctgggagacg gaggttgcag tgagctgagg ttgtgccatt gcactccagc ctgggagaca 28620gagcaagact ccgtctcaaa aaaaaaaaaa aaaaatatgt cactaatctc taagatctct 28680gaataactct ctcagaatta gagtccatac agctctgatc ttttcttctt ttatgctgct 28740tgtttttcca aggcttttag tgatgcaaac ttatcattac tttaacaatt ccagctttcc 28800ttctatgtct tggttttaca cgacaagaag cccagaaact gaacttgctt actcagctcc 28860cgaggttgaa ggtggcaaga aggtggtaag aatcctagac aggctaggcc tggtggctca 28920cgcctataat cctaacaatt tgggaggcca agacaggagg atcgcttgag gccaggagtt 28980cgagactagc ctggggaaca tagtgagacc ctgtctctac aaaaaaaaaa aagaaaaaat 29040tagctgggtg tggtagtgca cacctgtggt cccagctact caggaggcca agttgggagg 29100attgcttgag cccaggaggt ggaggctaca gtgagcaatg atggcaccac actccaacct 29160gggcgacaca gtgagactct gtctcaaaac aaaacaaaac aaaacaaaac aaagaagatt 29220tggtagaatt ctcctgtggg agtttggaga aagaagtaga acattattat tgtttttttc 29280ctgtcatatt tctaatggaa acaggatgat tacaagagtg caggggaaag gttggggaaa 29340agaaatgtga cttctttcac ttttacatta ttgaaattgt tatttaaatt gtattctata 29400accataatta aatcttcata aacttttcac ttaatgatct tagcattcat tgatggtctt 29460tgcctgaatc ttatttcaaa aggcaaggca tttcaaaaat gctgattttt aaaatttcta 29520tcatttctta tacacttatt agctcacatt tttctgtaat gagtttaccc ttatcaagtg 29580ggactctttg gttagctgct cctctaagct aattttcttg tattctcaaa gcagtttctc 29640catactatca tttcaatacc tcttgctgtt ctagtttgct gttttgagta tgtccttgtc 29700ttacttatgt gattgacttt ttttttgaga tggagtttct ctctcgtcgc ccaggctgga 29760gtgcaatggc accatctctg ctcactgcaa cctccgcctc ccaagttcaa gcaattctcc 29820tacctcagct tcccgaataa ctgggattac aggcacctgc caccacaccc agataatttt 29880tgtattttta gtagagacgg ggtttcacca tgctggtcag gctggtcttg aactcctgac 29940ctcagaggat ccacccgcct tggccccaca aagtgctggg attccaggcg tgagccacag 30000tgcctggcca tgactaactc attccattga gggtcttttc tcctgaagtt ttgtgctatg 30060acttgatatt tcaaaagaag ggaaatagat gtctcagtat taaatttcaa acgggaagtt 30120taacctgtat attggcttat ttagggtaag agtgaagcta tcctggacaa gaactttgac 30180aggacaatac tattcactct gaaggaccaa aaaatgagca gaaaatttgg gataaatgtc 30240aacaagattt gaaccctaaa gaaaagcaag catcgagtta gacagacgtc catattcatt 30300caactgggaa aataaaactg cagtcccaac atcagaattt ccttggtcag ttggttattg 30360cgggacagca ttcttctgca tgacagtatt gtctccttac cagccacaga ggaggctgtg 30420agagagagga tttgggaaat tggtgcaaat gagaaatctt ggaaatctgt aagtatatag 30480atataaaagt tattctttcc agtttgattc ttttatgatg tagattttaa tatcagtcaa 30540tttaggaaac tctgtggctc tgaattatag ttataattct agttttacta ttacagtgaa 30600agaagagaag aggctgttca ttattatatt ggaagtagtg tagcatgttt attaagagtg 30660cagagcccca gcatgaagcc tagctctgcc atttgccagc tgtgtgtgct cttgggcaga 30720ctacttatcc tctctgtgcc tcattttcat ttgtgaagta ggggacggtg tgtagttccc 30780acctcacaga gtggttgcaa ggaccaaatg ggttaataca tgataaatgc ttagtttagt 30840gtaagttcaa taaatatcaa aatagtggta tgttaaagat agtgtttaca tgataaccta 30900aaattaattg ccagtttgtt ttaatttact ggtcaagtct atcaaatgat tgaatcagca 30960tgtttaagtg gatatatctc atttgtgtcc agatcatttt agtatattca tgactcctca 31020ctttaaaatt caaatgataa taggtacagt tagtcctcca tatctgtgga ttcaaccaag 31080taaaaataaa aaattgcatc acaacaaggc atggtggctc acatctgtag tcccagcact 31140ttgggaggct gaggcgggtg gaccgcttga ggccaggagt ttgagaccag cctggccaac 31200atgacaaaaa cccatctcta ctaaaaatac aaaaatcagc tgggtggtgg tggcttgcac 31260ttgtagtccc agctactcgg gaggctgagg gatgagaatt gcttgaaccc aggaggtgga 31320ggttgcaatg ggccgacatc tcaccactac actctagccc gggcaacaaa tcaagactgt 31380ctcttttttt tttttttttg agacgaagtc tcgctcagtc gcccaggcta gagcgcagtg 31440gcgcaatctc ggctcactgc aagctccgcc tcccaggttc acgccattct cctgcctcag 31500cctcccaagt agctgggact acaggcgccc accaccacgc ctggctaatt ttttgtattt 31560ttagtagaga cggggtttca ccgtgttagc caggatggtc tcgatctcct gacctcgtga 31620tccgcccgtc tcggcctccc aaagtgctgg gattacaagc gtgagccacc gtgcccggcc 31680aagactgtct caaaaaaaaa aaaaattgca tctgtactga acatgtacag acattcttcc 31740ttttcattat tctctaaaca acagagtaca actatttaca taacatttac gttatattag 31800gtattataaa agtctcccga gagacagagg ttgcagtgag ccgagatcgc gccattgcac 31860tccagcccgg gggaccagag cgagtcttcc tctcaaaaaa aaaaaaaaaa ttgagatgat 31920ttaaagtgta caggaggatg tgaataggtt agatacaagc acaataccat tttgtatcaa 31980agacttgagt attcataaat tttggcatct ctaggaggtt ctgacaccag tctcccaggg 32040acgctaggga cgcctgtata tggcttagat tcagtgtgtt agtgaaacct gctacacagt 32100agcctgttta gagttcccca tttttaaaaa tactctgctc ttttaaaatt cattatacag 32160ccttatttct cagtactgac taaaatgtct tatttttata tatcgaagct ttctatttat 32220tttttaaacc aatgtataca tgtcaaatcc taaaaatcgc ctgtattaat ctacttagta 32280acttaatgcc actccaatgt ggatataaat agaacttgca catagttttg aaactacgta 32340gaaagcatgg aggctgggtg cgtaggctca cgcctgtaat cccagcactt tgggaggcta 32400aggtgggcag atcacctgaa ttcaggagtt cgagaccagc ctgaccaata tggcaaaacc 32460ccgtctctac caaaaataca aaaattagcc tggcatggtg gcatgcacct gtagtgccag 32520ctacttggga ggctgagaca ggataattgc ttgaacctgg gaggcggagg ttaccgtgag 32580ccaacgtggc accactgcac tccagcctgg gtgaaagagc gagactctgt cttaagaaag 32640aaagaaagcg ggcggggggg cggggggaag ggcaagcaag catagagcat gaggaagttt 32700ttaatgtctt tttcttacag agaactaaag cctttcagca ggaaatccag atgctcacta 32760agtgactaga gcagctgcat catctttaag aagaaggtgc tcaagaatca tcccaagctg 32820aagaaaaaag tattgggaac aaaaaagacc cttgaaatgt ctagaaagaa aaattgagat 32880caaggacttt ttcaagagag attagacttg gacaggaaga aagtaagaat ttcctgaagt 32940ataagcattc ctttgataat gaaaatgatt gcattttatt cataacttta actttatcta 33000atgtttgaag ctgttaatac tgttaatact tttctccaca ttgggaaagg ggggaaattt 33060gctacaaact ctgaaagctt ccgattttat tttatttatt tatttatatt ttttgagaga 33120gagtctcgct ctgtcaccag actggagtgc agtgacgcta tcttggctca ctgcaacctc 33180tgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagtagctgg gactacaggc 33240acgcgccacc atgcccagct aatttttgta tttttagtag agacgaggtt tcaccatttt 33300agccaggatg gtctcaatct cttgacctta tgatccacct gcctcagcct cccaaagtgc 33360tgggattaca ggcatgagcc actgccctgg cctctgattt tatttttaaa agcctcttct 33420ctctccgtat ctccatgtct cttttgtgta cttatttgat gtttgttgtg agggcatctg 33480tttacatata tatctcaatg tactttaagg agaggattag aagaaaagga gctcaaagga 33540ataactctct ttttttcttt tttttcagat ggagtctcac tctgtcaccc agggtggagt 33600gcaatggtgt gatctcggct cactgcaacc tccgcctccc aggttcaagc gattttcctg 33660cctcagcctc ccaagtagct gggattacag gtgctcacca cccgcccggc taacttttgt 33720atttttagta gagacaaggt ttcaccatgt tggccagtct ggtctcaaac tcctgactgc 33780aggtgatctg cctgccttgg ccttccaaag tgctgggatt acatgtgtga gccactgtgc 33840ccggccaaag gaataactct ctaatgggga aattttagga attgtgacag gcagatataa 33900tgagcattga tgaggggcca ttgatgatgt ctctcaataa tcactgtata agtcattctc 33960ttctctactc tctgcttccc tgaatctgta aggaaaaagg cagtcctaaa agttggatag 34020aaataggtag gttgcaatac aatttatttt caggagattc tctattttac tacctcttca 34080tagaattgcc tatcatagcc gggcacagtg gctcacacct ataatcctag cattttggga 34140ggctgagaca ggcggatcac gaggtcagga gattgagacc atcctggcca acatggtgaa 34200actctgtctc tactaaaaat acaataatta gctggatgtg gtggcacaca cctataatcc 34260cagctactct ggaggctgag gcaggagaat tgcttgaact caggaggcag aaattgcagt 34320gagccaaggt agtgccgcta cattgcagcc tggtgacaga gcaagactcc atctcaagaa 34380aagaaaaaaa aaaaaaaaaa gaattgccta tcataaccaa attacgttat agtatttcta 34440taattgctat ggtccaaagt ggaatcttgc tcactcattt accatttact catttagtct 34500tttttttttt tttttttctg agactgagtc tcactccatc acccaggctg gagtgcagtg 34560gcgcaatctc ggctcactgc aacctccacc tcccaggttc caatgattct cctgcctcag 34620cctcccgagt agctgggact ataggtgtgt gccaccacac ccagctaatt tttgcagttt 34680ttagtagaga tgcagggttt caccatgttg gccaggctgg tcttgaactc ctgacctcgt 34740tatctgcctg cctcagcctc ccaaagtgct gggattatag gcatgagcca ctgcgcccag 34800ccttcattta gtcttttggc tcattcattt gtttatccaa tatttattga gcattcagtt 34860ttcttttctt ttcttttttg agacggagtt ttgcccttgt tgcctaggct ggagtgcaat 34920ggcgcgatct tggctcactg caatctctgc ctcccgagct caagcgattc tcctgcctca 34980gtctcctgag tagctgggat tacaggcatg cgccaccaca cctgtctaat tttgtatttt 35040tagtagtgac agtgtttctc catgttgttc aggctggtct cgaactcccg acctcagatg 35100accagcccac cttggcctcc caaagtgctg ggattacagg tgtgagccac catgcccggc 35160ctaatagttt tctttactag agttcttggg ttttctggat ttcactgtat attgtcaaat 35220tgtttcctag gaaataatgt atttttaaaa tttcacttat ttattttata aataatttga 35280ttctaaaggt ggaacagttt ggaccagctc atgggagaac tgtctttttt tttttttttt 35340tgagacagtc ttgctctgcc tcccaggctg gaatgtggtg ttaccatcac gattcactgt 35400agcctcaacc tcctaaggct caagtgctcc tcttgcctca gactcccgag tagctgggac 35460cacaggtgca caccactatg cccagctaat ttttgtagaa atggggtctt tccatgttgc 35520tcaggctggt cttgaactcc ttgtggctca aatgatccgc ccatctctgc ctcccaaagt 35580actggaatta cagcctttat ttcttttaga ttttcaattt actgccccta agttgcaaaa 35640tgttctctta gaattatttt tatctttgca ttctgtatcc attctgtatg catatctata 35700tattctgtat attcctcctt tctaatattg tgtattttca ctttctctct ttccttttta 35760accaggcttg cctgaggcat ctattttatc tttccaaaga accagtggtt ctttttgaga 35820cagggtctgg ctctgtctat tgtccaggct ggagtgcagt ggcgtgatta tggctcactg 35880aagcctcaac cttcagggct ccagtgatcc tcctgcctca gcctcccaga tagctggaac 35940tataggtgca cgcctccacg cctggctagc tttttgaagt ttttgtagag atgaggtttc 36000gccatgttgc ccaggctggt ctcaaactgc tgagctcaag tgattctccc gcctcggcct 36060cccaaagtgc tagtattaca ggcatgattc accgtgcctg gccagttctt tttttaaaat 36120atacgtttta tggcctggca cgctggctca ccctgtaatc ccagcatttt gggaggctga 36180ggtgggcaga tcacttgagg tcaggaattc gagaccagcc tgaccaacgt ggtaaaaccc 36240tgtctctact aaaaatgcaa aagttaactg ggcatggtgg tgtgcacctc taatctcagc 36300tacttgggag gctgaggcag gagaatcact tgaaccaggg aggtggaggt tgcattgagc 36360tgagattgtg gcactgcact ccagcctggg caacagagcg agactgtctc aaaaataaat 36420aaataaataa ataaatacac attttattag tttattttgg tttatatgta atgaatatat 36480atagtatgtt tattaaatct gcaccctact ttctcttttc ccttcttaag taaatgtgct 36540ttttttgtct tttgttttta aatcagaaaa agagtaagta ctattgaatg ttcctctgta 36600gcttgtccaa tagattttta taagaaatgt ttcttttcac tgtgttctat gtatttttgt 36660aattttagtg ttgatttcca ttttttggct gaaggtattc agaatttttg tttgtttgtt 36720ttttgttttt gagacggagt ttcactcttg ttgccaaggc tggagtgaag tggtgcaatc 36780tcggctcact gcaacctctg cctcccagat tcaagcaatt ctcctgcctc agcctcccta 36840gtagctggga ttacaggtgt gcaccaccat gcccagtgaa tttttgtatt tttagtagag 36900gtggggtttc accaccttgg ccaggctggt ctcgaattcc tgacctcagg tgatccccat 36960gcctcgacct cccaaagtgc tgggattaca ggcgtgagcc accgcaccca gcctagaatt 37020tttttaaccc tttatacaca cacacacaca cacacacaca cacacacttt tcaaaatcaa 37080atataccaag agtcttttat tgaaaaggag cagccctctt ctgtacctct cttatttccc 37140agagggaact ctttaactct tttagctgtt tctgatagta acttccattt tcctaaacaa 37200ttttaaactg ccttatctcg agttatctat attagacatg ttgttgattt cctgttatat 37260gatagatgaa atttcatctc tcataccact ttcctacctg ctcctttcat cttcccaatc 37320tggttatatt gatattttaa gctaaatgca taatcagcat ttactttacc gtgatactaa 37380atgtttacaa aagcatgaag tactatattc ttggtacatt ttctttcttg tattgcattt 37440tatttttcct acagttaata ccttccttat attttcatac gtttaatttt cagtgttcat 37500ttatcaaggt ttttttcctg cctaaatctg tatcagatga tcctttagtc tttaaaaatc 37560tcccattttt tctctcccaa agtccttcat tcccttgctt cagtctggat tttactctgt 37620gggcctgagc acagccatga tgccatgact ttccttctct aatctcctgg ttaggtttca 37680ttgtttgccg aatcacattt cttcctcttt tttggtttcc tttcttattt tactggaaca 37740catcctccaa tggcttccta agaaatggtg ccttggagag cgacattttt tgaggtcctt 37800gtgtgaatga caatgtcttt ttttgtttga atcatcatac tcaattgata gctgggttta 37860gactttattt tcacacacgg acttcattgt cttctaatct ctagggttgt tattgaggaa 37920aatatgccat tctgattctt gttccttaat atgttatttc ttgtaatgga ttaaaacatt 37980taccataact tttttgtaat aacattttat aactaatttt tttctcataa taaaattaat 38040tcagctgggc atagtggctc atgtccgtaa ttccagcact tagggaggct gaggtgggtg 38100gatagctgga acccaggaat ttgagatcag cctggacaac atggcaacac cccatctcta 38160caaaaaattt aagaactagc caggcatggt gcacacttgt agtcccagtt actggggagg 38220cttaggtggg aggatgggtt gagcacggga ggtcgaggct gcagtgtgct gtgattgcac 38280cactgcactc cagcctgagc ggcagggtga gaccctgtct caaaaaatta aaaaatcatg 38340tgtattagtg aagatttgta aagtagaaaa taatagaaat aaaattgaaa tcaccagtaa 38400tcccaccatt ttgcgataac tagtattaaa tataggtata tttccctctg attattttcc 38460atgtatattt ttatcatcat tgagatgtac atagtttttc ttaatcctgc ttttttcatt 38520aactacatca ttgttaattt ttcttaataa atatttttga aaacttaatt tctagggaat 38580aatctatcat ataataattt ttcttttttg acatttgcat tgtttcagtt ttttctatta 38640tgaataatgt tgtaaagaat atctttgtgg gccaggtgcg gtggctcatg cctgtaatct 38700cagcagtttg ggaggccaag gtgggcggat cacctgaggt caggagtttg cgatcagcct 38760ggccaacatg gtgaaaccct gtctctacta aaaataaaaa aattagccag gcatggtgac 38820aagcgcctgt aattccagct actcgggaag ctgaggctgg ggaatcactt gaacctagga 38880ggcggagatt gcatttagcc aagattatgc cattgcactc cagcctgggc cacaagagcg 38940aaactctcag atattcaaaa agaatatctg aatatcttta tggccgggtg cagtggctca 39000cccttgtaat accagcacct tggatgagca acgcaggagg atcacttgag cccaggagat 39060tgagggaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag cccgggtgtg cacaccctgt 39120agtcccagct actccagatg ttgagatggg aggatcactt gagcctggga ggtcaagcct 39180tcagtgagct gtgataacgc cactgcattc cagcatgggc gacagagcaa gaccctaact 39240taaaaaataa aataaaataa ataatatctt tgtgtgagtc tttttttttt tccccgagac 39300ggagtttcgc tctttcgccc aggctggagt gcagtggtgc aatctccgct cactgcagcc 39360tccgccttcc ggtttcaagc gattctcttg cctcagcctc ctcagtagct gggattacag 39420gcacctgcca ccacgcccag ctaatttttg tatttttagt agagaccggg tttcatcatg 39480ttggccatgg tctcgatctc ctgacctcgt gatctgcctt cctcagcctc ccaaaatgct 39540gggattacag gcttgagcca cagtgcccgg cccgtgtgtg aatctttatc cacatatttg 39600attatttctt tataatacat tcctagaaaa gttgaactaa tctatgctac tgcttggtgc 39660ttataaaatc tatttcattg tactcttggt agtcctgagt catcactttt attgtcatat 39720tttattttct tgattatttt aataatgctt atttatggtg aaaaatattt tacttatgga 39780agtattgtta agcctgcagt gtgttagtct ggagtggatg tggtattatg ctagtgtata 39840ataacatctt aaagcacaat tttttacttc aatactgttg atttctgcaa aggaaaactc 39900agggactaag cattcccaga tagacaagca tagcaagaag ttcaaaaagc tagagaaaga 39960caacatgcaa caaaacaaat gttattgaat atttggcaga atttgcagat tgctactacg 40020caaagattgg aggagaaaat tcagaaactt cagaaacagc tcagtgattt gaaattgtca 40080aataaaaata tgaaaactca gctgacaaga gtaaatgtcc ttaaagtaag taaaggaagt 40140gaggctttca gaaattcact aaagctttgg tcttttacca ttttagcagc atgcttttca 40200ttgaagctaa aaaaattaat attaaaatta ttttacatac atacaaaaga atatatattt 40260aagcatatag aataaaattg gaattcacca ctcattttaa gaaacaaata ttaccaatac 40320aattgaaacc cccatatatc catatatcct cctccttgta ttctaagtgt tattattaca 40380aatcccttgt tttttgtttt attattatta ttctttttga gacagagtct tgctctgtta 40440cccagactgg agtacagtgg cgtgatcttg gctcactgca acctctgcct tccaggttca 40500agtaatcctc ccacctcagc ctcccaagta gctgggaata caggcgcatg ccaccatgcc 40560cagttaattt ttgtattttt agtagaaaca gggtttcacc atgttggcca ggctggtctc 40620aaactcccga cctcaagtga tctgcctgcc ttggcctccc aaagtgctgg gattacaagt 40680gtgagctacc tcacccagtc tgttttttgt tttagtttca cttgtgtctc ttattggggt 40740aatagttaaa agttgtagtc attttgggga ctcttctttg gaccttctcc atatcaaact 40800tcagttatac tatttcaaaa attatgcctt aagccaaaca ggcactaaat aactgatcaa 40860ctgatatgcc agtttatcag caatatttct cttactgaaa tgccaattat cttctgtatt 40920tctgtatttg attaatgtag ggctttaaga cctttgagga tgcagaaaac atgttaagac 40980tctttaaatc ccaagtggcc agttaataaa agtgctaagt aatagttatt gatgagcacg 41040atcatggaga aatgtcctct taattcagaa attttggaat gtttttcttt tccatatatc 41100tctgtcttca taaaaaaaga aagaaaagga aaagaagcaa gagttatcat aactgttatt 41160tagaagagat ctaatcctag ttatttcctt cctttccccc atcttatatg ctgtcacagg 41220acaaaacaat tgaagagctc aggcaatctt tagcaaatgt tgaaaggatg aaagagaagg 41280caaatgttga aacgatgaaa gagaaggcag ttgtgaaaac agaaaacttg aaaactacat 41340tagactctgc agagcaaaag gcaagatcag acaaagagaa gacccagcag atgttagatg 41400ctgtcacttc tgagccccca acagcaaaga gcgcacctga agaagtatca ggacaagaac 41460aagaggtttt ttcaaaatag taaaattaaa attaatttag ttgaagtgat aaacacccac 41520gcctacttag actgtaacta acttgacaaa agggaatatt ggcttatgga atcatgagtt 41580aaacaaccta acctccaggt gggcggagat acacctgggc cccaggatca gcacctggaa 41640cctacaagcc ctttctcatg gtcacttctt ggcatctatg tgtcagctca tctgtctgac 41700tgctggttgg ttttctccat gacaggaaac atgatcactg gcaaccatgg agatttatat 41760ggagtttctg tgacaggaga gagaatccca agaaaaggtc ttattgaccc agtttaggtc 41820aagtgcttat ccctgctaaa cccacagtgg ctagggatgg gatatatgat tattttatat 41880actctaacaa gtgaccagga gtgtagagtt ctatgacatc atgtgtcatc atgtgaagct 41940caggggaaac catgtgtgtc actttctgtg tcactgtcta ggagaatata gtagtgcttc 42000ttaacttgct tagattttca ctatacttat aaagcaaata tttcgagaga attttttaaa 42060ggccaactga tcatctgggg caatttttaa attatatgtc acttctccat atattagttg 42120cttatgaaac ttatacctta agatatgctt aaaacataaa gtcatgatat tagcattttc 42180acttttttct ctctttaact tctgtgtgtt cactttcttg attgtttcta ttcctctctt 42240ttattttttg agacagagtc tcactctgtt gcccaggctg gagtgcagta gcatgctcat 42300ggctcactgc agcctcgacc tcctgagcta aagcagtcct ctcactgcag ccttccgagt 42360agctggggct ataggcgtgc caccaccaca cctaattttt gtatgttttg tagagacagg 42420gtttcaccat gttgcccagg ctggtctcaa actcctgggc tcaagcaatt ctcctgcctt 42480ggcttcccaa agtgctggga ttacaggtgt gagccaccgt gtctagtccc ttattttatt 42540ttcagtcaaa tctgttttcc tttggtcacc ttgtagttca ctcttcactt tttagatgat 42600ttagtgtttt cttgcctgag ttcaactctc acatctagat atcttggact attctgaatt 42660attacatttc tgattcaaaa taaagttttt ccccccattt ttgcaaatcc tttaaaaatt 42720gatatttaat ttttttcagg ggtgttgtgt caaatatttg tcctgtgttt cacggctgtg 42780tttttttttt ttgttttttg tttttttttt ttgagatgga gtttcgctct tgtcacccag 42840gctggagtgc aatggcatga tctcggctca ctgcaacctc cacctactgg gttcaagtaa 42900ttctcctgcc tcaacctcct gagtagctgg gattacaggc agtgctacca tgcccggcta 42960atttttgtat ttttagtaga gatggggttt caccatgttg gccaggctgg tcttgaactc 43020ctgacctcag gtgatccaac ttccttggcc tcccaaagtg ttgggattac aggtgtgagc 43080caccatgccc ggcccatggc tgtcttttgg gagtattttt tttttcatcg cctgaacagt 43140ttagatgata gtgtctttct attttcttct taaagtaatt ttatgtgaat ataatttgcc 43200atttgtctgt attcactttc aaattccttg aattgctctg gtcttcagat gtttctactt 43260tagggtagct ggaggcaggg cttactcact aggttcctta gctcaatact accctcttct 43320gttggcacag tgagtgcagt ttctaaagtt tactagatca agcctttttg ggagtgaggg 43380tttatatgat gtctgattct gtaatactgt ctcatttgta taaacatatt aatttccact 43440gtttgctttt ttcttttcct ttattaccaa gcctccaagg aacacaacta cctccctctc 43500ccctcagaaa ccttgccctc tacaactgcc atttttggtc tcatgtgctt ccaagaccct 43560tgcttttcct tcatttgtca gtgttctgat ccaccagatc tcagatttgt tcttggtatt 43620tcaccattta tgatctgtct tgtttctggg ggtaaggttg tctgtgttct acaccagtga 43680aaattagctg caccttcctc tgtagctgcc tctgctggtt tagaatattt atttccccac 43740taacatgcaa attgaagttt gtggtattct ctagttttgc tttaggcatg atttataggt 43800agtttttatt tgatctccgt gttgatcact atggtttttg gaggatgggt agaaaaatgt 43860gtttttaggg gactggtatt atcattcagc aaatcagaag tccaaaacgt aattacctta 43920tggatgaaaa ataacaaaat aagcagcaat aaaattaagt ttactctata aaagtgtaaa 43980agcaagaaaa aatttaaatg caaaggaaat tttaaataga gttattgtaa tagaagagac 44040catttttcta gcttaaaaat attgtttgaa agtagaatat attacattta ataatgttta 44100ataagactaa atttctagta ccaattcttt gtacataata cctgggtgac ccaaactaat 44160aacatatctt gatttctgta atggttgttt gtagcttagt gactttcaag aaactattct 44220cagctactca ggaggctgag gtggaggttg cagtgagcca agatcactgc actccagcct 44280gggcgacaga gtgagactct gtctcaaaaa ataataaatg aataaaattt aaaatttaaa 44340ataaaaatgg aaactgctca ccttccgagg tagttttaga tgtacccaag atatcctctg 44400tgggaacaag tcagaatctg gacaggcctg tgggactcga tgcctgtttt agagagctct 44460tcagcatggg cggggtgaga ttatactgga ccttacagaa gtaccatttt ggaacaaaat 44520aattatcttg aatattcatt caagggataa atgagaatca ctttccaaat ggccacagcc 44580atgattccca aactgtgtgc caaggcacac tggtgcactg taaggatctc agcatgccat 44640ggaatgtttt gattttattt atatatttat tgagatggag tcttgctgtg tcacccaggc 44700tggagtgcag tgacgtaatc tcggctcact gcaacctccg tctcccaggt tcaagcaatt 44760ctcctgtctc agcctcctga gtagctggga ctacaggggc ctgccaccac gcccggctaa 44820tttttgtatt tttagtggag acggggtttc accttgttgg tcaggctggt ctcgaactcc 44880tgacctccgg tgatccaccc acttcagcct cccaaagtgt tgtgattata ggcataagcc 44940accgtgccca gcctaaatat tttgatttta aagggaagca ctgtaatatt tgacatctgt 45000caggaaggta catggatact agcttcaaca ttacatcacc cttcattctt tttgatgaca 45060tacctttgtg atagaaactg cccaaggctt ctcaggattt ataaatttgt tgccgagtga 45120acttcagtag ttagtggtat cgctaccaaa gaagctgtgc tgcaaacttg gggaaggagg 45180ctctttcccc agattctagg atccctggct gcaatgtaag aaacttgctt atccttcctc 45240tcaataatat caccagccta gcaaaagagg aaaagtgagg gacaccttga ccagagcctc 45300cacttgcagc ccagcctgct caagctgtaa gaaccacctt cttattcaga atttccccag 45360agccacggcc caagggtcat ctgcctgctg cagccttagc acacaggagc ctgggcatga 45420aacaggcaga ccctctcctc cttccaggac acccttctct caggaaatgg aggccctacc 45480agctcctaca gccaagctct ccatgaaggc caaggtgtta aggtgggcca gtttctgaaa 45540gagccacttt ctgagcatat taaagaaaaa tctcttgtaa aaacaaagag ggcttagtgt 45600taatgctcct ttatgaattg tgttagtagg aactgattgt tcaatcatgg ggcagtgcaa 45660aacgttagct gtgttaactc tgagcaaaca ggggagatcc ttcaaggacc tggaacactg 45720tttcctagaa aagcttttaa tcatgtgtcc aaatcactgc cacactcttc tttaatgata 45780agaattggat ttagatttaa ggctttagat aaatgattct caaccttcaa gatgctatga 45840aatgacctaa ttaaggaatt attttttcct aggcctatcc agagattcca gttcagtttg 45900tggtatgagg ctgaagtatg gatatttttt tcaaagctcc ttaagtatat aaattgataa 45960aattactttg gaagactggc agtgtacgag ttttctgttg ctgctgtaac aaattatcat 46020aaactttgtg gctaaaacag tacaaattta ttatcttaca gttctagagg tcccattggg 46080ctaaaattaa agtgtcaaca gggtgtgttc cttctggaaa ggggaaaatc catttgtttg 46140ccttttccaa cttctagagg ttacccacat tcttgcctca ttgcctcctt cctccatctt 46200taaaactagt aggaattgat caggcccatc cagtctttct cacagctact gttctctggt 46260tctttatctg attctacaat ttctctgatt ctccttctct tgcctccctc ttctctattt 46320ttaagggtgc ttgtgattaa gttgggccca cctggacaat ccagaatgct ctccctattt 46380taaggccacc tcttggcaaa ctctgtaaac ttaattctct tttgccatgt aacctaatat 46440actcataggt tccagggatt aggacaagga tattttgggg gacccattat tttgcttgct 46500gtagttagca tctactaaaa ttgaatacat gcatgcctta tgaagacgta gtcccacttc 46560tacatacatg ttagaagcaa atttttcagt gccacaaaat aaaagaaaat agcactcgaa 46620tataaatttt ctcagcaagg caaatttact ctttcaggag ggtgcccctc gtaggtctgg 46680ttgccacgag aggacgcaca aacaaaggaa agcagggggt tttattatct ctaatgcagc 46740ttgtccctgt tactgcgtct tgcctccatt ggctggagtt ggaccacacg atctaagctg 46800aacctggttg gctaacttga aaagtgcagg aatgcggttt tcaagtggga aggtgggaag 46860atcaattttt ctgggaaagc tgttacagca gggagggggt gatttcttgg ctgtcttgcc 46920tgagcacagc aggatgggag gggctgatag attggcaggc aagattactg tagacaaaga 46980acagagaaat aagacttcag gacagacagt acaaggaagt aaagacctct tggagaagaa 47040taccttgttt gtaacaaagt gaaactcttt gaagaggaac tgtctaaact acttgttttt 47100aacatatata acagaaatgt gtacatattc ttttttaaat ttttaaattt tattattttt 47160ttggagacag agtttcgctc ttgttgccca ggctggagtg taatggcacg atcttggctt 47220actgcaacct ctgccccctg ggttcaagtg attctcctgc ctcagcctcc agagtagctg 47280gtattacagg catgcgccac cacacctggc ttattttttg tatttttagt agagacaggg 47340tttctccatg ctggtccggc tgatttctcg aactcccgaa ctcaggtgat ctgcccgcct 47400cagcctccca aagtgctggg attacagacg tgagctacct cgcctggcca gaaatgtgtg 47460catattctat acaaagacat ttacaatatt actaacggtg gcattgttca ttattgccgg 47520aaactggaaa ctacccaaat gaacaatggt ggcttaagta tggcaatcag atccattagt 47580taagcattcg atctcatttg gggttggaca gggagaggtc aactggagtg ctgaattttt 47640ctgaggccaa actagaaagt aactctagga gctgggcgtg gtggctcatg cctgttaatc 47700ccagcacttt gggaggccaa ggcgggcaga tcacctgagg tcgggagttg gagaccagcc 47760tgaccaacat ggagaaaccc cgtctctact aaaaatacaa aattagccag gcgtggtggc 47820gcatgcctgt aaatctcagc aacctgggaa gctgaggcag gagaatcgct tgaacctggg 47880aggcggaggt tgccgtgagc cgagatcgtg ctattgcact ccagctgggc aacaagagtg 47940aaactctgtc tcaaaacaac aacaacaaca acaaaacaac aacaacaact actactctag 48000gaccaggact gggagatggc aaatagggga gagcatggag ttccactaat aaaatggtaa 48060tacactaaca gtggagaaag caatgggctt gtaaggagaa cttggttctt gttgaacatc 48120cgtcactaat cagtcttgca acccttgatc aagtcttgtc acctcactaa actcgaattt 48180tctttatctc aaaactagaa agaaatcgtg gcctggattt ctgaattatg gggtttcagg 48240cagccacaag taggctggca gaaatgtttt ctggtcaaat aaaggagtct gttgccccag 48300aacaggagcc aggggcacag ccaacagtaa aattctgctt ggggccccat tcctcagctg 48360caaggtactt gtcttcaggt gtggcaggct gagatctgcc tccttgttcc cggcctatcc 48420cgctgggccc ctcccctgca agtgttcgct tccatacaga gggaccatta gggcctgtac 48480atcgtacttc aggtcccctc ctgtggtcca acattcccta ggggcaagtg caactgttta 48540tgcggagacc acttttccgt gcagcccaac tgaaggtgca cgttccaccg caacctggtg 48600cttacaacag ctcgggaggc cgcgctaccg cgcctgcgcc cctctgaata tggaagcgcg 48660cgcgaggtcc ttaagggaaa aggaagtaag ggcggggacg gagggagacg acttcaacgt 48720agggagttgc tgctgctaca ctgccggccg gagaggacaa ggaaaacgtg gaggaagtcg 48780gtgatgactg gctgaagggg atgattggcg ggtgaaaaga gccgggccag aaagcacctt 48840tgcatgtggc tagaaacccg cctgaagagg ggctgaaacc cacgccggaa cccgcccgat 48900tcgagccaat cagggagagg agccgggtgg gggggcggac ggggcggcct cgcggggggt 48960ggacggggcg gtctgcgggg agggggggcg gtctgcgggg agggggacgg ggcggcctcg 49020cgggagggag gacagtttcg cgggttcggg cggcgagtct cccggatgct cctcagctct 49080ggggacgcgg tgcagaagtg tgagggcgcc cggcttccag gcagtaatgg gcgggtccct 49140gcgcgggagc gtggcgggcg ctggactcta cagcagatgt ggaactggag agcttggcgc 49200gccttccgac tttgtcacac acctgcgccg ccagactggg gtcgggcccc tccgcgttct 49260gctctggagt gcctgggtct gggcccagca ccgcgctttt agaatctcct cagctgaatc 49320tgacgctcag cagtgggtga agcgcagccc cctgtttcag gccctgccga gctggaagga 49380gtgtcagagc tggagcgcgc gtggccccct ctgtgttggg gtcaccccgg ggttgccagg 49440gctcagggag ggtcgtagtc tggattttgt cacccgcacg tccccacccc ccagcaggtc 49500tggggttgga gaatccacgc gggcttcata agctagatgc cagttaactg tcgagagggg 49560acgctccctc ctcgtaggcg tccacactgg agaaggaata agatgggcga ttgcctggga 49620agcctgacag ggcggcggca gctgggatgc tggagaggac tggccccttg agttactgag 49680tccgatgaat gtgcttgctc tgctggagga accgcgctca ggttacagtc atcccaatat 49740ggttctgaag gtgcgtggtt caggtcactt aggacttgac cagataccgg gtttctttta 49800caagccgttt ctgacggtgg cctgtttcaa ctactggcag agctcatgta aaacagactt 49860ttaaaaaaat ttggggggct tttagtattt ttttcttatt cctatattct gaggatattt 49920tatagtagtc ccacatatgg aattagataa tctctttttt gtttgattaa cagttttatc 49980aagtataatg tacataccat aacgttcacc cattttaatg gattcaatga tttttagcat 50040atttacagag tggtgcaacc atcagcataa tagaattaag gaatcgtgat tttttttttc 50100tggtaattgc ttttacagtt ctcaaagttt gcacaagcgg atattttaga ggtacagtgt 50160aatataagag cttctgaaaa tgtccactta agttgtttta tacctgagca agtgaaatta 50220agaagggaat tgaagcaaat attcctggta agttgtaggg agtgaaactt ttgtgtcttg 50280taataccaag tagatattga ccatttcaac tggtttttat gctgaggaaa tgcataaacc 50340ccattttaca gatgatgaaa tcgactttga aggataagtt gcctacagct gcatacctgt 50400gcctgggcta ggccccaaac ccagatgctt tatctctcaa tttgttaccc ttgctacctc 50460aacagcttgg ttttcaacca tggtactgat gagtatgaac agtacaagcc attcatttac 50520tgagcaaata attattgagt gccactctgt gccaagaaca ctgctatagg tgctagagat 50580attattgaat cagataccgt agtgaactgt tcctgccctc agctcatctt ctggtgggga 50640ggacaatgat caagtaaaga aatatatagt tttagagatt catctatttt tttaataggt 50700aaattaaaag ggcaaggaat ggcagtggga ggcagaatct gatgagaaaa atctgaatga 50760agagaggaag ttaggatata agaaagaaag caagggtttg atttgagcaa gcgcaaaaat 50820agagttgtga tttactgaat tgaaataagg tgatactgga aggaccaggt tttgggggta 50880caatcataag tttggcttta aatgttttta aataccttgc ctcttagaca tccaagtgga 50940gatatggcat ttaaattcat gagattggat gagatcccac caaaggaaca ggtttaggtg 51000gagacaacca aataccgatg cctaggacac tgcagtgttt agaattcaag gagatgagaa 51060ggaaacagga gggaagattg aaaagaagag tccagtgtgt tatgaggaaa accccaagag 51120catgctgcct tacaagacag gtgaaaaatg tgttctgtga aagaaagagt aattaactgt 51180taaatgttac agactgatca aataaaatga agactgagaa tggcctgttt gtaaggtaat 51240aaaaatacat aaaatcttat gatagaaata tttatacata aagttagtaa ggaaacagtg 51300tttactcctt tttgtagaag tgtaaatttt tacaaccatt ttgaagggca gtttgatatt 51360atctacaact taaaattgtg cttccattga taatttcacc tgtggaagtt tatcctacaa 51420aaatattaat atgtgcacac aaatatgtgt aaaagtgttt atcacagctt gtacacatat 51480atatttataa atgtgttgtc caggaacagt ggcttatgcc tgtaatccca gcactctggg 51540aggccgaggt ggatggatca cctgaggtca ggagttcgag cccagcctgg ccaacatggc 51600gaaaccccgt ctctattaaa aatacacaca cacacacaca cacacacaca cacacacaca 51660cacacacaca caaattagct gggcgtggtg gcggacgcct gtaatcccag ctacttggaa 51720ggctgaggca ggagaatcac ttgaacccgg gaggtggagg ttgcagtaag ccgagatcac 51780gccactgtac ttctagcctg ggttacagag tgagacttca tctcaaaaaa aaaaaaaaaa 51840aaaaaaaagg tgtttatcac agcattgttt acatttgtaa aaaggtacaa gttttcatca 51900agatggatgc agttgttaaa gggaagatat aaatgtgtag atatgggaga tagctgctat 51960agacggaatt gtgtcccctg aactttcata tgttgaagcc cttaccctga atgtggtggt 52020atttggaggc agggcctttg ggaggtagtt tgatttagat gaggtcacgc agatggggcc 52080cccacgatgg gagtagtgtc cttatacaaa gaagaaggga gtccagagct ttcttctgtc 52140agtcatttaa ggacatggtg agaaggcagc catctgtaaa ttaggaagag tcctcaccag 52200gaactgaact ggctgtcacc ttgatcttgg tctttccagg ttccacagcc atgagatatg 52260aatgtctgtt tttaaagcca ctcagtctgt ggtattaata ttttgttata gcagcccaag 52320ttaagacaga tagctttgtt aaatgataaa gtcaggttat ctaatagaat gcatagtata 52380accccattta tcttaatgta tcacaggagg cctttctagt cacactaaca aaagttactc 52440ctttgtgtgc cttccctgat cactgttaca ttattctatg tacagcactt attatctaaa 52500attatttcat taatttttat acatgtttac tggcttgtca caatagaagg taagctctgt 52560aaggggtttg cctctcttgt ttatatcccc agtgctaggt atatattact ttaggaaaaa 52620ccattattta ttaaaaatat tttaggaaaa aaccctacac aaacagtatt cctgtagtgg 52680ttttaaaata agacaacagg ctgggcgtgg tagctcatgc ttgtaatccc agcactttgg 52740gtggccgagg caggcggatc acctgaggtc aggagttttg agaccagctt ggccaacatg 52800gtgaaacccc gtctctacta aaaatacaaa agttagcctg gcctggcgtc acacgccttt 52860aatctgagct acttgggagg ccaaggcagg agaatcactt gaacccagga ggcagaagtt 52920gcagtgagct gagatcgcac cattgcaccg tagtctgggc aacaagagca aatgtctcaa 52980aaaaataaaa taagaccaca atttctttga tagtgtttcc ttccaaaggt ggtggctaat 53040tctcctcttc ttgaatgtag gctggattta gtgacttgct tctatgtgta gaatatggcc 53100aatgtggagg tatgtcaata ggtcatgaat tcctttttgt tctctctctt cgatcattca 53160ctctgaagta aagcagctgc cttgtcatga gaacatatca aacagtgctg tggaaaggca 53220catttggtga gaaataggcc tactcccaac agccagggaa gaactgaagc cttctgtgac 53280atgtgaatga gccacctgag aaatgtattt ttcatcctca gtcaatcagt gtctcaaaag 53340aggccgttag ctggatccct caacaaagcc acttttgggt tcctttcaga taatacaggt 53400ttgctttgta atctactagg tttggtggta gagtgagaag actgaacaca ctccccttta 53460ggacacatca taaagcaaaa caagtatggc ccaaagtagc atacacttaa tgttcttttc 53520tactaggatt tacagaattc attgttggta caatttactc ttttaaaaaa taatttttat 53580gttgatcaga ataaaatacg gtattccaag ctatatgtgc taacttgatt ttattttaaa 53640aatgtattga acactggaac acacagattt gaaagatttg accttaatat atatttatat 53700ataaaatatg attttgaaat aatgaacttt taaatttaaa attataaata atttttaaaa 53760tgccttctat ttaggtaaag aatcttcaaa acaaacttct catatgatat ggtttgtctg 53820tgtccccacc caaatctcat cttgaattgt agctcccata attcccacat gttgtgggag 53880ggacccagtg ggagataatt gaatcatggg gggtggtttc ctccctgttg ttctcgtcgt 53940agtgaataag tctcatggga tggttttatt agggatttcc cctcttgttt ggctctcatt 54000ctaccttgcc tgttgccatg taagatgtat gtttcacctg ccatgattgt gaggcctccc 54060cagccatgtg gaactgtgag tccattaaac tttttattta taaattaccc agtcttgggc 54120atgtctttat cagcagtgtg aaaatggact aatacatcat aaaagaaatt tcattgcaaa 54180agttgaagtc tgaactaaaa agctacaaag aaaataatgt ttaatagcca tcccagatag 54240tgtccctgaa atacgatgtc aaggatctag aggaacatat tgtatcttta accagaatta 54300agtctgaaaa acaagtattc agagtcttaa aagaggcaag caggacttaa cggaacgaat 54360tataaaacta aggtagaaaa ttctagttta tttttgaaac atgtctctca tcataagctc 54420acatatagca tatgagctcc atgctcctga ttgatcagtt taatttcatg gaatttcact 54480tattgcctgg tataacatta ttacaatttt tcattataag acttgtgatt atcaaggtca 54540ggatatcaag accaacctgg ctaacacggt gaaaccccat ctctactaaa aaatacaaaa 54600aattagctgg gcgtggtggt gggcacctgt agtcccagct actcgggagg ctgaggcagg 54660acaatggcgt gaacccagga ggcagagctt gcagtgagct gagatcgcgc cactgcccta 54720ccctccagcc tgggcgacag agcgagactc tgtctcaaaa aaaaaaaaaa aagatttgtg 54780attatctggt caatgtgtgt agagaggaga tgtttgatca tatacggtac cctttttttt 54840ttttttgaga tggagtctca ctctgtcccc caggctggag tgcagtggtg cgatctccgc 54900tcactgcaag ctccgcctcc tgggttcatg tcattctcct gccttagcct cccgagtagc 54960tgggactaca ggtgcccacc agcacacctg gctaattttt tgtgttttta gtagagatgg 55020ggtttcaccg tgttagccag gatggtctcg atctcctgac ctcgtgatcc acccacctcg 55080gcctcccaaa gtgctgggat tacaggcgtg agccaccgtg cctggttaca gtaccctttt 55140tgatagcagg agaaaagatg gtcattaatg tatcctctta taataagagt aatatttaag 55200aaagccacaa aatatgaaaa gcttttctat ccagatttac attctgttgt agaccatctt 55260tattctgtta tttactgtac gttagaccaa ttgatacctt tcattttcct ctggggtttg 55320catttcgcag atcactttta aaaggaaaac ataggagcct gaaacagaag tgggaaacaa 55380atatttactc aaactaagag actaaactca gtagccagca acaagagatc aaggtgtgtg 55440tgtgttttct ggttgtgcag atattgtctg aaataagatg gctgaaaagt tcaagtgaaa 55500aagtaattaa aagcaattca tcaaccatag ccatagctgg atgtataata gctgatcagg 55560catagcaaac tcttcaggat aatttcattt ttaaaaattt atgtctttgt ccttttcatc 55620ttctaagcac agtttcaaat aagactacag agtgaggctc tagggaccat cagtttttgt 55680ctttagtgct aaaatggtgg ctgagtgaca caccatgatt ttttttctca atatttcatc 55740attctaccag tgttggaaaa gggagagaag gactctctga aggagactgt gcaaaggatt 55800cttctttttt tttttttttt tttgagatgg agtctcactc tgttgcccgg gctggagtgc 55860aatggcatga tctcggctca tgcaacctcc acctccctgg ttcaagggat tctcttgcct 55920tagcctcttt agtagctggg attacaggcg cgccaccacg ctcggctaat ttcttgtatt 55980tttagtagag aaaggatgtc accatgttgg tcaggccagt ctcgaactcc tgacctcgtg 56040atctgcccac ctcggcctcc gaaagtgctg ggattaccag cgtgagccac tgggcccggc 56100cccaaaggat ctttttacac catgtctggt tcccagccct ttttctatcc ttcctgtgca 56160gtgtggactg agttgactga gatatttagg cccaggactt cttgcttgtt ctatagttat 56220tgagaaaagt gtgtcaaaat atccatcact gattaaggat ttgtctgttt atttagttct 56280atcaacattt attttttaac tttgaagcta tttgcataca aattgaggat ttttatcttt 56340ctattgaatt gcccctttta tcgttatgaa atctcactta tttcatgtaa tactttttgc 56400cctatagtct aggttgtctg atattaacat agctagataa tatttcttag attgcatggt 56460atgtattttt ccatttttca ttttcaatct ttctatgtga ttaaagtatg tcttttgtaa 56520acagcatata gttttgtttt ttaatctagt cttataatct ttgtctttta attggaatgt 56580ttaggctatt tacattaaat tctgatattg ttggatttaa gtccaccata ctgctactta 56640ctgtgttttt tctcctctgg tctttgttct tgtaataatt agtttgtttt ttgttattgt 56700tgattttttt ttttttttgt caagatggag tcctcctctg tcacccaggc tggaacgcag 56760tggtatgatc tcggctaact gcaacctcag cctgccaggt tcaagcaatt cttctgcctc 56820agcctcccga gtagctggga ttacaggtgc ctgctgccat gatgattaat tttatgtgtt 56880aacttagctg ggctgtgttg cccagatagt tggttaaaca ttattctgga tgtttctgtg 56940aagatgtttt tggatgaggt taacatttag atcggtggac tttgagtaaa gcagattacc 57000tttcataatt tgggtggggc tcatccaatc agttgaacat ctgaagagac caaaagactg 57060accttctgca agcaaagaaa aattctgcca acagacagcc attggacttg aacttcaaca 57120ttgactcttc agtctattgg cccaccctgc aaattttgga cttgccagta agtgtctgaa 57180atctagtgag gcaatttctt tctttttttt tttttttgag atggagtttc gctcttgttg 57240tccaggctgg agtgcagtgg tgcgatctca gctcaccgta acctctgcct cccaggttca 57300agtgattctt ctgcctcagc ctcctgagta gctgggatta caggcatgtg ccaccacgcc 57360tggctacttt tgtattttta gtagagatgg ggtttctcca tattggtcag gctggtctca 57420aattcccaaa ctcaggtgat ccacccgcct tggcctccca aagtgctggg attacaggtg 57480tgagccacag tgcccagcct aatttctttc tttctttctt tctttttttg agacagagtt 57540ttgctctttt tgaccagaaa ggagtgcaat gtggcaggat gttggctcac tgcaacctcc 57600acctcctggc ctctctagta gctgggatta caggcgcctg ccaccacgcc cagctaattt 57660ttgtattttt agtagagatg gggtttcacc atgttggcca ggctggtctc aaactcctga 57720aattacgtga tctgcccgcc ttggcctccc aaagtgctgg gattacaggc gtgagccacc 57780atgcctagcc gggtagttta tcttgacttg acttcaggct caccaatcct tttggctgca 57840attctacgat agaaaaggac ataaaaaact ttaaattagc cttagaataa agagatgtta 57900tcattcccta gcaattagta ttcaaagcaa gatccaaata tgtaattagt catttatgta 57960tctaagctgt ttgtatgtat gatacaagtt ttcacataca aatttcttct ttctttcttt 58020cttttttttt gatagaggca gggtttcacg acattgccca ggctggtctt gagctcaagt 58080gatccatctg ccttggcctc ccaaagtgct gagattacag gcatgagcca cagtgcctgg 58140cccaaattat tgtagttatt tccaattcct ttcccccttc tcacatccca attaaagaat 58200tccactcagg aattgttgta gtagaagtgc tttagtctgt gtgctacggt ttggatactg 58260tttgtttgcc aagtctcatg ttggaatttg atcactaatg ttgaaggtgg agcctggtgg 58320gaagtgtttg ggttgttaag gcagatccct tatgaatggt gtggtgccct tctagaggga 58380gtaagttcgt tctcactctt ggttcccaca agatctcgtt gttgtaaaga tccttgtact 58440tacccctcct ctctctcttg ccttctcttt caccatgtga tctacacaca cagtatcata 58500aggcatcttt ctgatccttt agtgttcact ctccagtacc tttaatattt gccttcaaat 58560ttctcaaatt tctttattta cttccatttt tctcctacaa taattgtagg cgtacttaaa 58620gtagaattac aatataaata atattttaaa atatctacaa ctaatactaa aggggttact 58680ttattttatt taaattttat ttttaaataa gaatttaaaa tatctgcaac taatatcaga 58740gccaaggggc tactttcttt gaaatacaaa gagtctttag agtcagactg tgtatgtttc 58800aatctgggat ctacctctta tattgtaggt ttagacaaat tgctaaatat ttcttgtccc 58860agttttctca tctacaaaat ggaaaaatta gcttcccttt gctgtctgcc ttgagtagaa 58920gcttcctgag gccctcatcc aaaacagatg ttggtgccat gcttctagta cagtctgcag 58980aactgtgagc caaataaacc tcttttcttt ataaattact cagcctcaag tattccctta 59040tagcaacaca aatggactga gataccgtgt gtgatgtcct aatccttata atattatcct 59100actacccagg cagatattgc tctccaaatg tcttcttaaa aaggatggtt tctgaaatga 59160caccctcttg ggactattgg aattactgaa cagctgtttt cattagaaat cttttttttt 59220ttttgagaca gggtcttgct ctgtcgccca tgctggagtg cagtggtgca atttcagctc 59280actgcaacct ctgcctccca ggttcaagtg attctcctgt cttggcctcc tgagtacctg 59340ggactacagg tgtgcaacac cacacccagc taatttttgt gtttttagta gagatgggtt 59400tcattattta tttatttttt tgagacgaag tctcgttgtg tcacccaagc tggagtgcag 59460tggcgtgatc tcggctcact gcaacctcca cctcccaggt tcaagtgatt ctcctgcctc 59520agcctcctga gtaactggga ctacaggtgc acaccactat gcctggctaa tttttttttt 59580tttttttttt ttgtattttt agtagagaca ggtttcacca tgttagccag gctggtctca 59640aactcctgat ctcaggagat ccacccgctt tgcccttcca aagtgctggg attataggct 59700tgagccactg tgcccggcct tagaaatata ttttgactat acatatattt tgtttttatt 59760tatttttatt tttttgagat ggaggcttgc tctgttgccc aggctggagt gcagtggtgt 59820gatctcagct cactgcaacc tctgcctcct gggttcaagt gactctcctc cctcagcctc 59880tgaagtagct ggaattatgg gcacatgcca ccatacccag ctaatttttg tgtttgtatt 59940tgtatttttg aggtggggtc ttgctctgtc gcccaggctg gagtgtgtgg cattatcttg 60000gctcactgca acctccgcct cctgggttca agcaattctc ctgcctcagt ctcccgagta 60060gctgggatta caggagcccg tcaccacacc cggctaattt ttgtattttt agtagagatg 60120gggtttcacc atgttggcca ggctggtctc gaattcctga gctcaggtga ttcacctgcc 60180tcagcctccc aaagtgctgg gattacaggc gtttgccact gtgcctggcc aactatatat 60240atattttaaa aggggacatt tctttttaat tttggaatgg acatttgaaa attgtttgaa 60300ttactttagt ctactcatat ctttcagtct attgacacaa ggtatatctg gtttaaagag 60360aaaaggtgga acaaaaaaaa cccattctag atcaattggt agatgccaac agattcactc 60420ccatatgaat atgaaaggac aaggaaccat gaatattttc atgatgaagg tgagaataag 60480ttttgattga tttttgaaga aaaacaattt ttgttatctt gtttaactct aggaggtaat 60540cgagaaatgt tgagttgttt gttggttctc tcccaaaggg agggtagaag gaagccatgg 60600ttcctttata ccgtggttga ctgggagcct ttatgccttt ctgatatatt aagagaaaat 60660gcaagggggg cctaaaggtc tctgtgatac tgaagagaaa ggtatagggg taatagggct 60720gtgagaaagc tgaaagctga gatcatgtta cagaataaga tagcggagtt tcatatttct 60780ggtatggggc aattcctgct gatgacaaaa tccagggttg tttttggatc taggtgtagg 60840tggttgaagt agggtataaa ggcagtcatg tgctggtaaa ctggctcttg agaaaaagca 60900cccaatttga gcattcattg acttttgata ccaacatgtc attgagcata gaattagaaa 60960gagatatgaa taatcaactc ttgggagctg gaatgatctg gctttaacaa ccactttcta 61020catcaaaaaa agttaatgtt attaatatta gaataataaa taattaaata ataaatgagt 61080gtaggtgtag ggcattggaa ttaagtacac acatgaatca caaagctgta ttattggatc 61140gatcatctac tgtgacccct gaaatcttga attatggtat gagttggtat agaagaagaa 61200tgtgaggccc caaatcttca ttgagtgaag gagggttgag gagtagtcag tagaaaagaa 61260taaaaagaga agattttata gaagtctgtt gggggtaaaa tattgctgag gaagtaaaat 61320agtactgagg aagtgttctt caaattcctt cgactataac cactttttaa tgtaatctgt 61380atgtaaagca agggtctaca tgatccaatt tatgtgttgg ctccatttat aaaagaatat 61440ttcagttgtc aaaactagtt gaaagtacag ttaatccttt aataatctgg ggttagggga 61500tatggccatc atgcaaaaaa aaaaaaaatc tgtgtataat tgttgactcc tcccaaactt 61560aactactaat aacctgttgt tgactggaag acttaccagt aatataaaca gttgactaac 61620acatattttg tatgttgtat gtattataca ctgtattctt acagtaaagc tagagaaaag 61680aaaatgttat taagaaaatc ataaagaaga aaaaatatat ttactaatca ttaagtggaa 61740gtggatcatc ataaaggtct tcattctcat cgtcttcact ttgagtagtc tgagaaggag 61800gaaggaaagg aggggttggt cttgctgtct ccggggtagc agaagtagaa gaaaatccac 61860gttatcagtg gacccatgca attcaactcg gtcttcaagg gtcaactgta attccaatct 61920taattatttg ccttaactaa ttttcttaat aaaaggtgga atattcataa tttacaataa 61980caccttcatt ttcttaactt ttctcactat atctctcaca tcacatccta aacctttttc 62040tcctgtgcct aactctccat tctcttaaaa aactctccca gatccagtct atgctgctca 62100taatttctct tcccttcctc tttttcctac cttctttcta atgcaaattc atcatttcat 62160gaataatttt ctctcctctt atttcctact tttactcaac aaaagtccag aaactaaact 62220tgcttactca gatcccagag ctgcataaaa ggacaggaga tcttggatga tgtgtgggtt 62280ggaaacagaa ggtattacat tcttttgtta aataattgag gattttgcat gtggttaaaa 62340tgatgtcaga gctaggcaag gaaacgggat tctcctacat tcctgatagg agattaaatt 62400ggtacaaccc atttggaaat gcatttgtca atatctccta aaaccaaagt gtatccctaa 62460aaccagaata tatcctaccc tgtgactcag caattccact ccatcaacag tggaatgtaa 62520tgaatatggc tatcaggttt caatatgcta gtgacatctg ctacatctat taacagaagt 62580ctataatttt ttaacctctg atctctgaaa acttatttta tgactttatt actctacaaa 62640ctaaaatgtc ttactattgt gtatcagatc cacttctttt taaattaatt tttaaatgtc 62700aagtcttaat agtcttcctt tagcctctat ttactaattt attgtcccca caatgtcact 62760ctaaacgtaa ctgttaacta tcaggaagta tttccttctt tttctataga gaacagaaga 62820tcttcagcag gaaattcaga tgcttactca gcaaatggaa cagctgtatc atctttatga 62880acagctgttt gtgaatcatt ccaacttgaa gaaaagtata gggaacaaca aaagatcctt 62940gaaatacctg gaaggaaaaa ttgcttttaa tgatgtttta aaagattaga ctatgaaaaa 63000agactttcct gaattataga tgttattttg ggcaatgaaa ttaactattt attattctaa 63060tattaataac tttaacttta tttggtgtag aaagctgata aaaactattt atatttcact 63120taacattgga aaagtgaggg ggaaaaatcc ttagagttat gcttctaatt ttatcaaaaa 63180acatgccctt tcccatatct tcagtttttt caccgtgtac atatttgaca gataaaacca 63240tcaatataat atggaaagtt tagtgtcttt tataatctct tcttgtaagt tacataacat 63300cacttctgcc atattctatc agtcaaacag accaacctga tacaatgttg ttcgagacta 63360taagggtacc aggcggcgcg gtgcagatca ttggtggtca tcttaaaagt ctggctaccg 63420cactcatttc ttttactcac aaactgaatg atttgcttat tcattcattt attcaatact 63480tattgaacaa ccacagattt tacaaacata taggactggc tgggggcagt ggctcacgcc 63540tgtaatccca gcactttggg aggctgaggt gggctgatca cctgaggtcg ggagttcaag 63600accagcctgg ccaacatgga gaaaccctgt ctctactaaa aacacaaaaa ttagccaggt 63660gtagtggctc acatttgtaa tcccaactac ttgggaggtt gaagcaggag aatcgattga 63720acctgggaag tggaggttgc agtgagctga gatcgtgcca ctgcactcca gcttaggcga 63780cagagcaaga ctccatctca aaacaaacaa acaaaatagg acttaatgaa gtaaggtcaa 63840cagtagacca tatagagttt aaagataaat atatcatctc atctagcctc cacctctgcc 63900tttgaatatg tgtatggaaa taatacattg aatggttaat ccatgcaaat aaaaataatc 63960ctttattaag ttttcttaag attgtacaaa acgtgtgctt ggccaggcat ggtggctcac 64020acctgtaatc tcaacactct gtgaggccga ggtgggatca ctcgaggtca ggagttttaa 64080gaccagcctg gccaacatgg tgaagctctg tctctactaa aaacacaaaa attacctggg 64140cacggtagca catgcctgtg gtcccagcta cctgggaggc tgaggtggga gaatcatttg 64200aaactgggag gcagaggttg cagtgagcca agattgcacc actgcactcc agcctgggca 64260acagagtgag accctgtctc aaaaaacaaa caaaaaacaa aataacatgt gcctacccca 64320acacttaaag ctatgctaaa cagtttaaag gaaataataa ttttctctct gcccatgtca 64380cctcagtaac caatgttaac cattcccata gttatggaaa tatgtaaaca tatataaagg 64440gtaatggtgt cttcacaaaa ctaagatcat tctaataaaa atattctgca acttcctcta 64500cttagtagtg cctcatggtt gtatcttaag ttaaaagata tagctcttcc tttaataact 64560gtataatatt ctatagtatg catgtatctt aatttattca accatttctc ttttgaggga 64620tgatataatt atttccttct tttggtcact acaaataatg tgaaaataag tatctttcaa 64680cttatatcct tccacactgg tgcttttgtt gctaggggat taattgacaa atatgagctg 64740atagggtcac agtgcgtatt ttaaattcta atagccattg tcagattact atttgcaaaa 64800ggatagaagc agttcattta agagtaaatc attctccttt acatccagct agcattgaat 64860gctgtcattc ttttttgttg ttagttgggt aaaaaaagaa acaaaaaaca aggtacctca 64920ttattattgt aatttacatt ttcttgacta ctagtgaaga taaggatctt tttttttttt 64980tttttttcct ttctgtggag ataaggtctt actatgttac ccagactggt ctcaaacccc 65040tggatcaagc tatcctcctt tctcagcctc ccaaagggct gaaattacag gtgtgagtca 65100ttgcacttag ccagtaagca tccctcttct ttaaaaaaat aatttcaggc caggtgcagt 65160ggcacatgcc tgtaatccca gcactttggg aggtcaaggt gggtggatca cctgaggtca 65220ggagttcgag accagcctgg ccaagatggc aaaaccctgt ctctaccaaa aatacaaaaa 65280ttagctgggc atggtggtgg gtacctgtaa tcccagctac tcgggagcat gaggcaggag 65340aatggcttga acccaggagg cggaggttgc agtgagctga gatcatgcta ttgcactcca 65400gcctgggtga caagagcaaa actctgtctc aaataataat aataataatt tttattttta 65460ttatagatta aggggtacat gtgcaggttt gttacatggg cataatgcgt gatgctgagg 65520tttgggttac gtcaccaggt aatgagctta gtacccaata ggtgattttg catcccatgc 65580cccctctctc ccatgtctgg tagtccccag tgtctattgt tcccaccttt atgtttatgt 65640gtattcaatg tttagctccc acttataagt gagaacatgt ggtatttggc tttctgttct 65700tgtgttaatc tgcttaggat aatggctgcc agttccatct atgttgctgc aaaggatgtg 65760atctcattct ttttaatggc tggtaagcat cttcatatat gcctgttgac cactgggctt 65820ttcttttcta caaattgcct ccttcttccc ataatttgga tcttaggtgc agaagattgt 65880gctaatcaaa tttcttaaat agtgtcttgt cattggggac ataatggtcc atctctattt 65940aattttattg tttttggttc cattccccac ttccattcct tatgcccata ggtagcctca 66000cttaaatgtg tttatgtcta tcattttgtt tatgtgatta aaaaatcatt attgggatat 66060ttacatgcca taaaattcac tcatttaaag tctacaattc aatgattttt agtaagttaa 66120taaagttgtg caaatgccac cacaatccag gtttagaaca tttccatcac ccaaaaaaga 66180tttttttttt tttttgcttc tagacaatta atgccctctt ccatcactag tgccgggcaa 66240ccaccaatct gctttctgtg tgtatacatt ttcctttttt tggacatttc atagaaataa 66300ataactttaa tatgtagtct tttgcatcta gtttttaaaa ttagcattgt ttttgaggtc 66360catctatgtt gtagcattca tcagtattgt gttcttttta ttatttaatg gtattctatt 66420gtgtggatat gccacattaa aaaataatac tttatttttg gaagcaatta tagggttaca 66480gaaaaattga ctataaagta cagagatccc ataaacttcc ttccccatct tcacagtaac 66540aaattgcatt agtgtggtaa atttgttaca attgagttaa cattaataca ttattattat 66600tattattatt gaggcggagt ttcgctcttg ttacctaggc tggagtgcaa tggcatgatc 66660tcagctcact gcaacctccg cctcctgggt tcaaaagatt ctcctgcctc agcctcctga 66720gtagctggga ttacacacat gcaccaccac acccgactaa ttttgtactt tttttagtag 66780agacaggatt tcaccatgtt ggtcaggctg gtcttgaact gctgacctca ggtgatccgc 66840ctgcctcagc ctcccaaagt gttgggatta caggcatgag tcactgcgcc cagcctgata 66900cattattatt aactaaagtc cggggtttac attaggattc attctgtaat gtacattcta 66960tgggttttga aaagtgtata attacaagta tccatcatta catcatcata cagaatggtt 67020tcactgccct aaaaatgtcc tgtgttccat ctgttcattc cttcctcctc ctgcaaacct 67080ctggcaacca caactttttt tttttgagat ggatgtctcg ctatgttgcc caggcttatc 67140tcaaactcct gggctaaagc aattctcctg ccttagcctc ctgagtagct gggactacag 67200gtgtatgcca ccatgcccgg cttgatcttt ttactacctc cgtagttttg tcttttccag 67260aatgtcgtgt atttggaatc atacagatat aaccttttca gattggcttc tttcacttag 67320taatatgcat taaagttttc tccatgtctt ttggtggctt aatagctcat tgctttttat 67380tgcaatgtga ataaaaagca tttttttttt gcaaataata ttctgttgtg cagacttact 67440acattttagc tttccattta cctaatggta aatcttcgtt gcttccaatt tttgacaatt 67500ataaataaag ctgctataag cattcaagtg caggttttta tgtggacata aattttcact 67560tcacctgggt aaaaaccaag gagtactatt tctgagtctt attgtaagaa tatgtttagt 67620tttgttagaa actgccaaac tgttttacaa aatggctgtt ccattttgca tttccatcag 67680caatgaatga gagctattgc tgtcactctc acatcctcac cagcatttgg tgttgtcagt 67740gttctggatt ttagccattt gaataggtgt gtagtggtat ctcatcattg ttttaattgc 67800agttccctaa tgacatatga tgttgaacat cttttcatat gcttatttgc catctgtata 67860tcttctttga tgagaacttt tgttcagaac ttttgccatt tttaaattga gttctttatt 67920ttctagttgt tgaattttaa attttatttg tatattttgg gataacaatc ctttatcaga 67980tatatctttt gcaacaatta tctcccagtc tgtggcttgt cttttttatt ttcttaatag 68040tctctatcac agggcatact ttttagtttt aatgaagtcc aacttgtcag tttttttttt 68100catgaatctt gcttttctat tgtatccaaa aaatcatctc taaacctagg tcacttacat 68160tttctcctac gttgtcttct aggagtttta tagttttgta ctttacattt aggtctgtga 68220tgtattttga gttagttttt gtgaaggtgg tatgaggtct gtgtctggat tcattttttg 68280ttaatgtgga tatgtagttg tatgtagttg ttctagtacc atgtgttgaa aagactatcc 68340tttcttgatt gaattgcctt gttcctttgt taaagatcag actttggatg agtctatttc 68400tttaatttct ttcatccaag ttttaaaata gtcctcattt agactttttt tttttttgag 68460actgggtctc tctctttcac cagggctgga gggctggagt gcagtgatgc aatcacagct 68520cactgcagcc ttgacctcct gggctcaagt gatcctccca tctcagcctc cctagtagct 68580gggattacag gcacatgcca accacgcctg gctaattgta ttttttgtag agataggatt 68640gcaccatgtt gcccaggctg gccttgaact cttgggctta agcaatctgc ctgccttggc 68700ctgccaaagt gctgggatta caggcatgaa ccacaacacc tggctagcta atttaaaatt 68760ttttcttttg tagagatgga atcttgctgt gttgacctgg ctagtttcta attcctggcc 68820tcaaatgatc ctcccaccat ggcctcctgg ggtgctggga ttacagatgt gagccaccac 68880acccagcata ttttgttaga tttataccta agtatttaac ttgcttgata atttaaattt 68940tttttttttt tttttttttt tttttttttg agatggagtt tcgttcttgt cgcccaggct 69000agagtgtggt ggcacgatct tggctcactg caacttttgc atcccagatt caaaggatgc 69060tcctgcctaa gcctcccaag tagctgggat tacaggcatg tgccaccatg cctggctaat 69120ttttgtattt ttagtagaga cagggtttta ctatgttggt caggctggtc tcgaactcct 69180aacctcaagt gatccacctg ccttggcctc ccaaagtgct gggattacag gcatgaacca 69240ccgcacccgg ccgatacttt aaatgttatt gtgcttttaa tttcaatttc taattgttca 69300tatttggtat attaggaaag caattgactt tgtatattaa ctttgtattt tgcaaccttg 69360ctgtaattgt ttattagttc cagaaatttt aaaagtcaat tctttgggat tctctacata 69420gagaatcatg taatctgtga acaaaaacag tttcatttct tccttttcaa tctgtattaa 69480ttttcttttc ttttcttgcc tcattgcact ggctagatct tctagcattg tactgaataa 69540gaacaataag catggatatc ctgttttcaa tcttagaggg aaagcattca gtctttcacc 69600attaaatgta atgttaaata tagatttttt tatagatgct tgttatcaag ttgagaaagc 69660tcccctgtat tcctgttttt ctgagtttat ttttatgagt ggtgttgaat tttgtcatgc 69720tttttctgtg tctattgata tgatcatatg tttttctttt ctagcctgtt aacatagtga 69780gttacattga tttttgaagg ttgaaccacc cttgcatctc tggaattaag gcctgatatt 69840gtttggatat ttatgctacc caattctcat ggtgaaatgt aatctccatt gttggaagtg 69900tggcctggtg agaggtgttt gggttatggg ggcagatccc tcatggcttg gtgctgtcct 69960cacgatagtg agtgagttct cacgagatct ggttaattta aaagtgtgtg gctccctccc 70020tgtctctcta tcttgcttct gctctagtta tgtgatatgc tgtcaggtgc tgggctcccc 70080cttcaccttc tgccatgatt gtgagcttcc tgaggcctca ctggaagctg agcagatgcc 70140ccgcaccatg cttcctgtac agcctgtaga actatgagac aattaaacct attttctttg 70200taaattatcc agtctcaagt attttttgtt tgtttgtgtt gtgagatagg gtctcactct 70260gtcgcctagg ctgtagtgca gtggtgcgac ctgggcccac tgcaacctct gcctctgggt 70320tcaagtggtt ctcccacctc agcctcctga gtagctggaa ctacaggtgt gtgccaccac 70380acccggctaa tttttgtatt ttttggtaga catggggttt caccatgttg gtcaggttgg 70440tcttgaactc ctgacctcaa gtgatcagcc tgccttggcc tcccaaagtg ctgggattac 70500cagcatgagc caccacagtt ggcctcaagt atttctttat agcaatgaaa gaatggccaa 70560atacaacccc actttatcat ggtatataat tccttgtatg tattgctgaa tttgatttga 70620taatattttg ttaaggattt ttgtatatat tcatgtggta tattagtctg tagttatttt 70680attttatttt tattttttga gatggagtct tagtccattg cccaggctgg agtgcagtcg 70740tgggatctgg gctccctgta acttccacct tctgggttca agtgattctc ttgcctcagc 70800ctacaaagta gctggtacca caggtgcgtg ccaccatgcc tgactaattt ttgtattttt 70860agtagagaca gggtttcacc atgttggcca ggctggtctc aaactcctga cctcaagtga 70920tccacccacc ttggcctccc aaagtgctgg gattacaggc aagagccacc gtgcctggcc 70980acagttatat ttttttggat tgtctttgtt tggtttttat atcagggtaa tattagtttc 71040ataaaatgaa tttagaagta ttctctgtgt ctattttttg gaagatattg tgtaggatta 71100gtgttaactc ttcttttaag atttgataga attctccagt gagaccatcc ggatatggag 71160atttctgtta tgggaagttt taaaattata aattctggct gggcactgtg gctcatgcag 71220taatcccagc acgttgggag gctgaggcag gaggatcact tgagcccagg agtttgagac 71280cagcctgggc aatagagtga gaccctgtct ctacagaaaa aaaaaaaaaa ttagctgggc 71340atggtggcat gtgcctatag tcttagctac tcgagaagct gaggtgggaa gatgtcttga 71400gcctaggagt tcaaagctac aatgagctat gatcatgctg ctgcactcca gcctgggtga 71460cagtgagaca ctgcctctaa aaaataaaaa agtaaaaata aattataaat tcaatctctt 71520taatagttaa gggcaattaa gattatctgc ttaaggccag gcgtggtggc acatgcctgt 71580aatcccagca ctctgggagg ctgaggcggg tggatcacga ggtcaagaga tggagaccat 71640cctggccaac atggtgaaac actctctcta ctaaaaatac aaaaattagc tgggcgtggt 71700ggcacgcacc tgtagttcta gctactcagg aggctgagga aggagaattg cttgaacctg 71760ggaggcagag gttgcagtga gctgagatca tgccactgca ctccagcctg tcaacagagc 71820aagactccat ctcaaaaaaa aaaaaaatac aaaaaataca aaaaattagc caggtgtggt 71880ggtgcgtgcc tgtagtccca gctactcagg aggctgagac aggagaatcg cttgaacctg 71940ggaggcagag gttgcagtga gtcaagatgg cgccactgca ctccagcctg ggcaacagag 72000cgagactctg tctcaaaaaa ataaataaat aaaacattaa aaaaagataa cctacttaat 72060attggatgat tgtagtagtt tgtgtttttc aaagaattgg ttcatttaat gtaaattgtc 72120cagtttatgt gtgtagagtt gtttataata attccttatt attttttaga catctgtata 72180gtctgtagta atagaccttg cattctgaat actggtaact agcgtcttct ctctctctcc 72240tttttttttt tttttttttt tttgagacag actctcgctc tgttgcccaa gctggagtgc 72300agtggtgcga tcttggctta ccacaacctc cacctcccag gttcaagtga ttttcctgcc 72360tcagcctccc gagtagctgg gactacaggc acacaccacc atgcccagct aatttttgta 72420tttttagtag agatggggtt tcactatgtt gccaggctgg tcttgaactc ctaaccttga 72480gatctgcccg ccttggcctc ccagagtgct gggattacag gcatgagcca ccgcgtccat 72540ccagtcttct ctcatttgtg ctttgttagt cttgatagaa gtttgtcaat tttattaatt 72600tttctttttt tttttttttt tttttttttg agacggagtc ttgctctgtt acccaggcca 72660gagtgcagta gtgtgatctc ggctcactgc aacctccgcc tcccaggttc aagtgattct 72720cctgcatcag cctcccgagt agctggaact acaggcttgc accaccaggc ccagctaatt 72780tttgtatttt tagtagagat ggagtttcgc catgttggcc aggctggtct tgaactcctg 72840acctcaggtg atctgcctgc ctcaggctct caaagtgctg ggattacagg tgtgagccac 72900cgtgcccagc cgattttatt aatttttcaa aagaaccagt tctttgtttc attggttttt 72960ctattttttt cctgttttac atttaatcaa ttttgttctt atttttatta tttccttcct 73020tctgcttgct ttggatttat tttgttctta ttttcctagg ttcttggtgt gggagcatag 73080attattaatt tgagatcttc cctcttttct aatacacaca tttagtgcta taaatttccc 73140tcttggtggt gctttagctg tgtccctcaa gtgttgatat gttttatttt cattttcatt 73200cagttccatg tatttttaaa atttcccttg acctatgttt tatttaggag tacttgtttc 73260atttccatgt gattggagat tttcctgtta tctgttattg gtttctagtt tgattccact 73320gtggtcagaa atcacattct atacgatttc aattcttgta aatattttga tgtttgtttt 73380aatgctcagg atatggtcta tcttactatt tcttgcatag accctcaaaa ggttgtgtag 73440cctgctcttg tagggtggag tattctacaa atgtcaattg gattttgttg atgctggtgt 73500ggttgagttt ttctatgttc gtgctgatta tctatctcat tctatcaact gagagaggag 73560ctgaatcctc caacaatagt ggatttttct ctttcttctt tctttctttc tttctttttt 73620tttttttgag acagagtctc cctgtgttgc cctggctgga gtgaagtggc gagatctcca 73680ctcactgcaa gctccacctc ctgggttcat gccattctcc tacctcagcc tcccgagtag 73740ctgggactac aggcacccgc caccacgccc ggctaatttt ttttgtattt ttggtagagg 73800tggggtgcca ggatggtctc gatctcctga ccttgtgatc cacccgcctc agccttccaa 73860agtgttggga ttacaggcgt gagccaccgc gcctggcctc ttctttcttt tttcttttct 73920ttctttcttt ctctttctct ctctctttcc ttttcttttc tttttttttt ttttttgaca 73980gagcctcact gttgccccag gctggagtgc agtggcctga tctcggctca ctggaacctc 74040cgcctcccag gttcaagtga ttctcttgcc tcagcctcca gagtagctga gactacaggt 74100gtgcaccacc acatctggct gatttttgta ttttttatta gagatggggt tttgccatgt 74160tggccaggct gctttcaatc tcctgacctc aggtgataca cccgccttgg cctcccaaaa 74220tgctgggatt ataggcatga gctatcatgc ctgacctttt ttctttcatt tctatcagtt 74280tttgcttcac atatcttata actttgttgt ttgggggcat ttaagattac tgtgtcttct 74340tggttgattg atccttttgt tattatataa tgtccctccc tgtgtctggt aattttattt 74400gctctgaagt ctactttgtt tgacactttc ctttaatatt tgcataacat attttttcca 74460tcctcttact gtcaaattcc ttatattttt atttgaagag tttcttatag ataccatata 74520gttaaacatc ttttaaatcc cctctgctaa ctctgtcttt taactggggt atttattttt 74580atttattttt ttctttttgt gatggagtct cactctgttc cccaggctgt agtgtagtga 74640tgctcacttg gctcactgca acctctgcct cccgggttca agtgattctc ctgccttggc 74700ctcccaagta gctggaattg cagatgtgca ccaccatgcc tggataattt ttttgtattt 74760ttagtagaga ctggccaggc tggtcttgaa cttttgactg tatgggaaca gacacacaac 74820tctcccaaat aagcacaaca aagagacaca gaagcagtcc aagcctctga taaactctcc 74880catcctgaat ccttaaaaat gcttagtctg taagaggatg tgcctctgac ctaactcagc 74940cagacgcccc tctcaggttt gttttttcta aaataaacct gtcttgactg gcaagccacc 75000tttcttttct ctcctctttc tttaattcct acactgactt caagtgatct gcttgcttcg 75060gcctcccaaa gtgctgggat tacaggtgtg agacactgcg cccggcctaa ctggtgtatc 75120tagaccattt acatttaatg taattattgc tatattaggg cttaagtctt ccttttcatt 75180ttgttttctc tgttttttaa atttctgttt tctttttcct aatttcatgc ttgttcctga 75240aacatttttt agaattccat tttgaattat ttatagtttt tgatgataaa catatatatt 75300tggtatagct tttttagtgg ttgctccagg tattacattt tgtatatatg acttaataca 75360gtgtattgat gtcattttac cagtttgagt aaagtataga actcttagct tccattatgt 75420ctctactttt ccctgtttat ataattatct tagctatttc ctcttcatac atttagaacc 75480acatcataca gtgttatagt ttttgcttta accatcaaac atattttaga aaactcaaga 75540gaaggaaagc ctattgtatt tacccacagt tttgctcatt atattttctg tctcctgatg 75600ttccaagatt ccttcatttt taaaaatcat tttctttctg tttggagaac ttcattattt 75660agtaagtctt tttgtttttg tttttgtttt tttttagaga tggggtattg ctgtcaccta 75720ggctggagtg cagtagtgtg atcatagctc actgcagcct tgaactcttg agctcaagca 75780atccccctgc tcagcctacc aaatagctgg tactacaggc atgcaccacc atgcctggct 75840aatttttttt tttttttttt ttctgagatg gagtctccct ctgtcaccca ggctggagtg 75900caatggcgtg atctcagctc actgcaacct ctgcctccca ggttcaagca attattctct 75960catctctgcc tcctgagtag ctgggactac aggcacacac caccacacct ggctcatttt 76020tgtattttta gtagagacag ggtatcacca tgttggccag gctggtctca aactcctgac 76080ctctagtgat ccgcctgcct cagcctccca aagtgctggg attacaggcg tgaaccacca 76140tccccagtgt gttgtaggct tttaaaatgt aaagcaaaat tgttctacca gcagtgaatc 76200aaacagtagg ttttgaaacg tcaagaagcc caaacacaaa tttaagttag agttttgtaa 76260agtaatataa gttctccttt aaatgcattt taaaatatta ataattttct ttagtattgc 76320ttaaccccct gtaagtcact agggctccat aattattttg gaaccaactc ctaagttaat 76380attctttcac tgtaatttca gcatccttaa atcttctaag cacagctata agttgaaatg 76440attttagaga actgtgagta aaaatctaat atgataaaat ggctccattt tgcggggaag 76500gatgtactgg taattgacag aaaatgacca ggaacatgga aataggagta ggtcagacag 76560attgaattgt taagtatttt gaatatacta taaatgagat ataaatgata ttttgaaatc 76620aatatgcaat ttttgttgta tctaataagg acttttaagg atacagtcaa gaaggagaga 76680tgcaatatta ctgtgtttag ccttactaaa gcaaaggaaa gtactgtacg taaaagttct 76740ctggcgcggt ggctcatgcc tgtaatccca gcactttggg aggccgaggc gggcagatca 76800cgaggtcagg agttccagac cagcctggcc aacataatga aacctcgtct ctactaaaaa 76860tacaaaaatt agttgggcgt ggtggtgtgc acctgtaatt ccagctgctt gggaggcaga 76920ggcaggagaa ttgcttgaaa ccggaaggca gaggttgcag tgagccaaga tcgtactact 76980gcactccagc ctgggcaaca agagagaaac tccgtctaaa aaaaaaaaaa aaagttctcc 77040ggcatttttt gaaaaaggca aactgcactc ataaaatttt acctttggaa cagaatcttt 77100atagttacat aatcaatgga aagaacagat ttgatgacaa tattgagctt atgaattaat 77160caaatttgaa gctgctctac acccagaatt attattatta ttattattat tattattatt 77220ttttgagacg acgtcttact ttgtctcact ttgtcgccca ggctggaatg cagtggcgcg 77280atcttggctc actgcaacct ccgcctccca gattcaagcg attctcctgc ctcagccttc 77340cgagtagctg ggattacagg cacctgccag cgtgctcggc taagttttgt atttttagta 77400gagacgagct ttcttttttt taagacggag tctcgctctg tcgcccaggc tgcagtacag 77460tggcgtgatc tcggctcact gcaaactctg cttcccgggt tcacgccatt ctcctgtctc 77520agcctcccga gtagctggga ctacaggcgc ccgccaccat gcccggctaa ttttttgtat 77580ttttattaga gacggcgttt tgccgtgtta gccaggatgg tctcgatctc ctgaccttgt 77640gatccgcccg cctcagcctc ccaaagtgct gggattacag gcgtgagcca ccgcgcctgg 77700ccagagacga gctttcacca tgttagtcaa gctgtcctcg aactcctggc ctcaagccat 77760ccacccacct cggcctctca aagtgctggg attacaggtg tgagctacca tgcccagttt 77820atacccagtc ttgttaagtg agatgttaca tctccctctg tttagttcac ttgacgcaag 77880attctctatt tttttttttt tttttttgag atggagtttc actcttgttg cccagggttg 77940tagtggcaca atcttggctc attgcaacct ctgcctccca ggttcgagca attctcctgc 78000ctcagcctcc agagtagctg gaattacagg cgcctgccac caatacaata cttttttgta 78060tttttagtag agatagggtt tcactatgtt ggccaggctg gtctcaaact cctgatctca 78120ggtgatccac ccacctcggc ctcccaaagt gttgagatta taggcataag ccactgcacc 78180cggcctaaga ttctctatta cttgagaata aaacaacctg ttaaaatatt ataccacagt 78240gtgcttggcc tatgtaacat ctgcttagat aacatactct cttaagcagt aaatgagtat 78300gagttacagg ggctctcctt ttgttcttta gggactctag aaatgccaga taattccact 78360tttgtggtga cagaagaatc tggcaataat agctaccgtt tactgaacaa caactgcaca 78420ttaagcactg tgtcatatgc tttaggtatg ttatttgatc ctcaccaaat gcctaggtat 78480tattcctctt ttcttttctt ttcattttct tttctttctt ttctcttttc ttttttattt 78540tctttctttt taacaaagaa agaaactgag ggggctgggt gtggtggctc aggtgtgtag 78600tcccagcatt ttgggaagct gaggttggag gatcacttaa ggtcaagaat ttgaggttac 78660aatgagctat gctagcacca ctgcactcca gcctgggtga caggtgagac tctgtctcta 78720aaaaataaat aaatttacat ctgttcaaaa gataaatgac cttttaaaca aacaacatgt 78780agtataaagt ttatgacata caatcataaa aaataattaa taaaaaaaac agccaatgtg 78840acctgatatt tatagaacac tcttaacaat agcagaatac acatttttaa aagtacctgt 78900agaacattta tcaaaatagg ccatactatt tttctcaata aatttaaaat tatttctgtc 78960ataaaatata ctttctggcc acaatataat taaattagaa atcaataaaa aggatatcta 79020gaaaatctcc aaatgtttgg aaaataaaac ttctatatca cacattagtt tcaaaaaaag 79080aaattggaaa gtgttttgaa ctgtctgaaa attaaaacac aagataataa aacttgtgag 79140atacaataaa atagtgctag agggagtctt gtagcactaa atgcctatat tagaaaatag 79200gggcccggcg cggtgtctca tgcctataat cctagcactt tgggaggccg aggcaggtga 79260tggcttgagc tcaggagttc aagaccaacc tgggcaacat ggtgagaccg cctctctaca 79320aaaaatacaa aaattagctg ggcagggtgt catgcacttg tggtctccgc tcctcaggag 79380gctgaggtgg gagggtggct tgagcctggg aggttgaggc tgcactgagg catgttcatg 79440ccactgcact ccagtctgtg tgacaaagca agaccccgtc tcaacaacaa caacaaaaac 79500aacaaacaaa caaacaaaaa acgaaattag aaaaagagta agttaaacac agaataaaat 79560gaagacagga aataattaag attggagcag aaacttatga aatagaaaac aaaaatagca 79620ggaaatcaat aaagcctaaa gctggttctt tgagaagatc aataaaatta ataaatccct 79680aggccgggca tggtggctca cgcctgtaat cccagcattt ttggaggccg aggcgggtgg 79740atcacgaggt cagaaggtga gaccaacctg gctaacacag tgtaacccag tctctaccaa 79800aaatacaaaa aaattagccg ggcgtggtgg tgggcgcctg tagtcccacc tactcaggag 79860gctgaggcag gagaatggcg tgaacccagg aggcggagat tgcagtgagc tgagatcatg 79920ccactgcact ccagcctggg cgacagaatg agactctgtc tcaaaaataa acaaaacaaa 79980acaaaacaaa aaacaggtta aaagaccggt gtggtggctc atgcctgtaa ttccagcact 80040ttggaaggct gaggtgggcg gatcacgagg tcaggagttc gagaccaccc tgaccaacat 80100agtgaaaccc catctctact aaaaatacaa aaaaaattag ctgggcatgg tggcacatgc 80160ctgtaatccc aggtactcag gaggctgagg caggaggatc acttgaaccc aggaggcaga 80220ggttgcagtg agccgagatc gtgccactgc actccagcct gggtgacaga gcaagactct 80280gtcttaaaat aaataaataa ataaataaat aaattaaatt aaattaataa acctctagcc 80340agactgaaca gaaaaaaagt gaaaggaaac acaaattgca aatatcagga atgaaggaga 80400taacctacag attctacagc tattaaaata ataattagag aatattatga aaaacttttt 80460aacaaaaaat tcaacatata taaaatggac aaaccccttg aaaaaaacca aattaccaaa 80520aattgtacaa gaagagctga cctgagtagt cctatatcta ttttttaaaa ttgaatttgt 80580agtttaaaac cttcctacaa ggaaaactcc aagcccagat ggcttcagtg gtgaattata 80640ccaaatgatt aaggagaaat aacagcagtt ctctaccacc tctttcagaa aatggaagcc 80700aatggaatac ttcccaattc atcctaggat aacagcatta ccctgatacc aaaacctgac 80760aaagacattc ttagaaaact acagatcagt agtcttcagg aacacaggtg caaaaattct 80820caaggaaatt ttagcaaatc caacctaaca atatgtaaaa aggacaatgc attaagacca 80880accggagttt atttcaggca tataagtctt catttcaaag cccaatcaat ataattcact 80940acattaacat aaaattaaac catatgatta ccccaacaga tccaccaaaa gtgtttgaca 81000aaatctaaca tccgttccta ataaaaactc agcaaactag gtataggggc cctttgtttg 81060tctttttctg gtttccaagt ccttgaaaca aaatccaact atgtccaaat gccatgaagg 81120tttgtgttgc tgctgatgtc agagataaac attactttta aggacaggac ggagtggagt 81180agtagaagca tttagatgag aaaaaagaca aattaacttg tttaattctt cttaagagcc 81240aaaatgcagg tgtttcttgc acaatgtagt attctttttc tttttacttt cttttttttt 81300ttcttttttt ttgagatgga gtttcgctct tgtcacccag gctggagtgc aatggcggat 81360ctcagctcac ttctgcctcc cgggttcaag tgattctcct acctcagcct cccgagtagc 81420tggtattaca ggcatgcgcc accatgccca gctaattttt gtatttttac tagggacggg 81480gtttcaccat gttggtcagg atgatctcaa tctcttgacc ttgtgatccg cctgcctcgg 81540cctcccaaag tgctgggatt acaggcgtga gccaccgtgc ccggcctatt tttctgtagt 81600cccattttct tgcttcagag ttattcagga gttagcacgg tactacaatt gctatgcaca 81660gaagctgagg aacatttggt agtgttaaat acctaacatt gacttaaatc tgtacatagg 81720tagttctaga tatactatgc ttctttactg catcaaccag atggacatta aatggtagaa 81780ttatgactaa tttgtataaa gcattttata tagtatatat attttattta tttatttatt 81840tattgagaca gagtctcgct gtgttgccca ggctggagtg cagtggtgcg atcttggctc 81900actgcaagct ccgctgccct ggttcacacc attctcctgc ctcagcctcc caagtagctg 81960agactacagg tgtccgctac cacgcccgcc taattttttt gtatttttag tagagatggg 82020gtttcactgt gttagccagg atggtctcga tctcctgacc tcgtgatccg cccgccttgg 82080cctcccaaag tgctgggatt acaggcgtga gccactgcgc ccggcctagt ataataattt 82140ttaaaattag ctttaaatat ttttgagtta aaatcttgat attttaaaat gttgcccatt 82200aattaatttt tttttttttt ttttttgaga cggagtctcg ctctgtcgcc caggctggag 82260tgcagtggca cgatctcggc tcagtgcaag ctctgcctcc tgggttcacg ccattctcct 82320gcctcagcct ccagagtagc tgggactaca ggcgcccgcc accacgcccg gctaattttt 82380ttgtattttt agtagagacg gggtttcacc gtgttagcca ggttggtctc gatctcctga 82440ccttgtgatc cacccacctc agcctcccaa agagctggga ttacaggcgt gagccaccac 82500gcctggccgc ctattaattt ttataagcag tttgctttta atattttaga agaaaatagc 82560tctttgaata catttaaaac cagttttaac tttttaaatt ttaatacttt atttatttat 82620ttattgtttg tttgtttgtt tgacagaatg tctcgctctg ttgcccaggc tagagtgcag 82680tggaacaatc acagctcact gcagcctcaa actcctgggc tcaagccatc ctcccacctc 82740agcctcccaa gtagctagga ctagaggcat gagtcaccac acccagctaa tttttaaaag 82800attttttttt tgcagagaca tggtctcact atgttgccca ggctgatctc aaactcctga 82860cttcaagtga tcctcctgct tcagcctccc aaagcgttgg aggttacagg catcagctac 82920tatgcgcagg ttttaattta cttttgaata agtatgtgaa attaaataat tcaaacttaa 82980agctgttgga actttattct gagccttgag aggtgtgtgg ctgtgcagcc tgagtcacat 83040ggcatgcagc tgcaactttt gccttgtttt tcctttagat aattaagaac aaacagcacc 83100aaagaccccc acagatcatt acccctcctt atagagtaat aaagtattct ttcttggaat 83160ttagcaatct gtaaccaatc aaattgctgt ggcatatgca ctagtcttgt atgaaaagag 83220tcttgctctg tcgcccaggc tgcagggcag tggcagtcat agctcactgc agcctcgaac 83280ctgccgggct caggtgatcc tcccacctca gccctctgag tagctaggac tacaggcatg 83340caccactgtg cccagctaaa tgtatttttt gtagagatgg agttttgcca tgttgctcag 83400cctgtttttg aactgggctc aagcaatcct cccatctcag ccttccaaag tgctgggatt 83460acaggcgtga gccaccatgc ccggccaaaa ccaactaata ttaacagtat tttgtgtgtc 83520tctctaaata tatcctatgt gaatgtatgt atgtattctt tcttttgcct ttataaacaa 83580atgatagtat atttttcata acgttctgca ctctgatttt cttctcaatg tatcttggca 83640gtctttctca gtatatagtg acttttctca tttttttatc tttatacctc aatatctggc 83700acatagtaag caaatcataa atgctgagtg aatgaaatat taaatgaata aaaaggaaat 83760ttttgtgctg ctattggaaa ttagctctct atatatttca acatgttaca catatacaat 83820gatctaaaaa cttgtcttac tctttcctat ccactagagg gagacatcaa cctgttgtgg 83880aaaagaatga tcacttaaag tctttagaaa ttctgaacca actctctagc aggtgatcct 83940tgttagaatt tgagccctta acgctatcca ggactggagg ttgaagggac gatagaggga 84000gcaggaggag aatgcacatg gattaaggag cgagaacaca ggtgaacttc agcttttttg 84060ctaacagtca gacaaactac tgaccctgac tcagtgatgt gctagtaaac cagctcttta 84120aaaaaaaaaa aaaaagccct agattgctga tttgtatgta atgtttatga atttcagtag 84180agaaaaagac aatattcaaa ctgagccatg cacccaaaac aagagaacag ccaagaagtg 84240ttcacttcta tcagtgccct gggttgtttg aaaaaagaag ccgacctgag cacctgtgag 84300ctcccttctg gcgaggagaa atctggagtg tagttattcc accatggcca aattcaagcc 84360actcggggtt taatcaccga attgcaaatt ccttgaacat ttaacagtag gctctcttgg 84420ctgggcgcgg tggctcatgc ctgtaatccc agcaatttgg gaggccatgg caggaggatt 84480acctgaggtc gggagttgga gaccagcctg gccaacatag tgaaacccca tttctactaa 84540aaatacaaaa aattagctgg gcgtggtggc aggtgcctgt ggtcccagct actcgggagg 84600ctgaggcagg agaatcgctt gaacccagga ggcggaggtt gcagtgagcc gagattgtgc 84660cactgcactc cagcctgggc gacaacagtg aaactccatc tcaaaaaaac aaaacaacaa 84720taacaacaac agtaggctct cttgagccag cctgagcagg ctcttgcatg ctgctgaagc 84780ttgtcgggtc ttagttactt ttcctgtaaa gtggggatga taaatctgct cattatgtag 84840attctattac atagaggaca cataagttct ttgaatgctt aaagcaatgt ttcctaaact 84900tctttggtca tgaaatcacc cagtggcttg tgtaaaataa acattcccag gacctgccct 84960agagcacctg ggttagaaca ttttggggga gggggctggg aatctgtatt ttaaataagc 85020aacccaggtg aggccgggcg cggtgcctca cacctgtaat cccagcactt tgggaggccg 85080aggcgggtgg atcacgagat caagagattg agaccatcct ggctaacacg gtgaaattcc 85140atctctacta aaaatacaaa agaattagcc gggcatggtg gcaggagcct gtagtcccag 85200ctatttggga ggccgaggca ggagaatggc atgaacccgg gagacagagc ttgcagtgag 85260ccgagattgc gccactgcac tccagcctgg gcgacagagc gagactgtct caaaaaataa 85320ataaataaat aaataaataa ataaataaat aaataaataa aaataaaaaa taaaaaagtg 85380acccaggtga ctcttatgac cctgtgaaat gggagaaaca ctgctgcaaa ttactcttat 85440aattgggtca ggtgtcaggg gtctttctct aacttcacaa ttgggcctgc ttgaagagat 85500gtgtgcagag ttccacaaca cactccaggc aggcatttaa tccgttcact gtcttctcta 85560ccctcagagc ccaaacttcc caaagaggaa aacctgctcc ttgccatctc ttaggccaag 85620gcttctgtac acctgggaag tccttcaatc tgaggatctc tgggttgttt tcaagctact 85680atttattgag aatttacaaa gtgtcaggca cgttacagca atttgtcatt tctatgaaat 85740agcttcttgt gctattccca ttttacagag aaaaatcaaa gaagttggga aaatgtcgaa 85800gggcacacaa ctaggaagtg tttgtgctga aaacccaccc taggcccaag ccttggaact 85860ccaagcctgg gttccatccc tgcactgggc aattctgatc tatgtgcgct agtttccttg 85920tgttctctgt tctctccata gaaatcctgg gctctcttct cccagccaca aggttaggtt 85980gaaaaacaga gcagatggag gtagtttgta gcctacaggt gccctgaatg aagcttccac 86040agtgctaaag tggaagaacg agggactcca agggaaggat tcaaggctgg gcccatgcac 86100ctgtgtaatt cagaagagac cccagaggag atcagcgccc tctaattagc cctggtaagg 86160agctctggga gttactgtaa ctctctcaga agaacccaaa catgcgggaa cgtgacttct 86220taccttctga aagtccacaa aattcctgat tgccaccatt aatttgtcac ttatcatttg 86280caacaggcat tgtaggttgt cttatgcatt tgtcttctcc cttcagctag tgtataaagt 86340cttagggaga ccagcagttc agagagaatg ggctttggtg tgaaacagat ctggtttgaa 86400ccctctgcta cttactagct gttgggcaag ttccttaaat tctctgagtc ttaatcttct 86460catctgtaaa atggagacat aaggagtacc cacctcattg gattgtttta aggataaaat 86520taaatagtgc aggcaaagga tttacaagca actgctgaat gaatggtagt tatagcctcc 86580tcctcatcat ctgtgagcaa acaccctcat atttccttgt gtctcaggta gacacttaag 86640gtattgcaag cattaaggga gcattgtcac aaagagataa atgcatgagg gcaagatgca 86700gtctcaaaga agagtgtttt atgaaagaat aaatgtaatg ctgagtgtca gaaaaaaatt 86760ttttttttta aagatgaggt atctatcacc caggctgaag tgcagtggtg tgatcttagc 86820tcactgaagc ctcaacctcc caggctcaag tgatcctcca gcctcagcct cccgagtagc 86880tgggactaca ggtgccacca cacctggtta attgttgtat tttttgtaga gatgggtttt 86940cgccatgttg tccaggctgg tcttgaactc ctgggctcga gcgatcctct catcttggcc 87000tcccaaagtg ctgggattgc aggcatgagc caccacaccc agcctgtcag aaaaatttta 87060aggtgaaaat aactaaagaa gttgttaaga attttctccc ttgagtggta ttttagactg 87120agatgaggga gggtagaggt aggatgagaa ggaagggatg gggtccggtt gaaaggcctg 87180tgagatagta gcagtgcaat atggcagatg ttgacagcct cagtgctagg aacacagaaa 87240ctgaatctct tgcaaggagg caggtgtgca tctgtatgga agtcagatga cctgtgttcc 87300tatgagtgca aatctggaaa acaccctcaa gtttccttgt cagcaaattg gtgataaaat 87360caacattgta gggttggtgt gaacatgcag catgatgtgg ccatgcaagt tctttgttaa 87420ctagaagcca gtgtcatgcc aggacagcag tcctcctagt aagctgtggc tggtggcgtg 87480gtagaatacg tggagcaggc tgaggaagca cttgacttga ctatgagcag aaccattaag 87540aagctagtta gctaaactgc ctggacagta gaaaaataat atgtgaggat gtaaaaggaa 87600gagaaacaat gtgaggggag aggagaatgc agagatcctg gcccatggaa cagcattggt 87660gatccttaag tagctgcatg aactacttgg agaagttcat tttctgttta taattcccag 87720caaaggagag gactgaataa gagagaagaa aacgattcct ttctctggtt aggttcatca 87780gatcaaacgg tgacatatgt gaaagaagca cgctctgtgc acaaaaaatc aagtctgtat 87840ttttataaaa gccatttctg ggctgggcgc ggtggctgac gcctgtaatc ccagcacttt 87900gggaggcgga ggcgggtgga tcaggaggcc aggagatcga gaccatcctg gctaccacgg 87960tgaaaccctg tctctactaa aaaaatacaa aaaaattagc cgggtgtggt ggtgggtgcc 88020tgtagtctca gctacttggg gggctgaggc ggaagattgt gccactgcac tctagcctgg 88080gcgacagagc gagactccgt ctcaaaaaaa aaaaaaaaaa agctatttct gtaatgagca 88140tcactggaga gttagttgct atgggtctaa aggacaatat gaggcagtta tagtaacttt 88200ccatgatatg aacaaagaaa ttgaaaatgt tagatacatt tacaagaaga tgtagaaaaa 88260actttagtca aaatttttga aatatttttt gaaatattaa actatgaaat cagacagtct 88320tatctatggt ctcaagccat gtctgtctgt accttttttt tttttatctc atttcaggga 88380atattacact ggctgactta ttaatatctt ctgagccaga aaatgtaagg aagctgcatt 88440ttcagaattg catttgagtc atttgtgaaa ttgcatatta caatttgccg ccatttctaa 88500cagtcctata actttttttt ttttttttct taactgggtg ttcacattca tgccaatgac 88560ctctaggggc tagtttctct tctagctcaa gagaattgct gcagagttgg aagtaaggac 88620aaaaatgtgt atgcttcatg tttgatttca aatgcataga aaattagaaa cttaaggtat 88680gcaagggatt tgtgtggaat ttaagtacct ttgaggggca gtggacagga caaaaagtta 88740ttttttacct gtttgtttac aaatagcaaa gatcaagact gaaacacatg agtgtgattt 88800agaaagagtt ggctgcaggt gctgcttgct caggtggttc atttaaactg caggtcagag 88860caaccttgtc tcatggtcct ggtgcccagg tatcaggttg ggtctgtctt gctgcttatg 88920tccttgttac cctctgaggg ccccagtcca acgcagatca ataaagaata agttacataa 88980atatgctcat aggtggtcat tcctagacaa gaaattgaca acatttcatt caacagtatc 89040tgggctctac aggacagaca tgcctccatt tatgcaacaa ataagaacag catctcatga 89100cagtggagaa aacatgggat gtgcaggtag gtaggtaaag ttgggtggaa actttcaccc 89160taccaaatgc acatgggtga ctttataaaa taaatgttag ctctctgagc ctcagttttc 89220ccatctgtaa aatagacagt cccagggaat tttcaaggat taaatgaaat aaaagtgaat 89280caacctatgc aagcctgcct actgtggtgt ccaggctaga aaaatgctca ataaatatta 89340ggtttgtttt tatttctaca aaagatgtga tcctaaagag ctctatccaa attcaagttt 89400caaatgtcaa atcacatttt gtgaacttta tgttcagttg agatgatctc tgacatatta 89460attagtaatc ctatcttttt cattcatcac caccaaaaaa aggtgttatt gcacgttcaa 89520ttaatctttc ccctttatta attccataag tgtagggttt tatctctcag attctcttaa 89580aacagaccaa tttataccca cataatataa ataagcttgt tcctataaca ctctggagca 89640gataactatc ccagaaccca aatcctccta cttggcttca agctcagaga ataaagcaac 89700aatccaaagg caccctttgg catgacaccc ttctagacat ctgtagcatt cctcctttcc 89760ctccactttt cctattagct tttgctttct tgccttttac agggttttgt tttgcctctt 89820ggtagtttct ttcctacgga aaattctccc tctgatcttt ccaagtcaaa ggcttcagca 89880aacatttgtt gaacgcgtgg attgtgctag gtgggtgtta tggaccatgg agaatgctag 89940agatgtaaga catgcgctgt ccaatcgcag cgcaggttgt gttgacaggt aagatgaggg 90000ctgtagggga gccaatgtgc acgttccact gggctaatgt gctcttcacc ttatttaggc 90060tcttggcttt gggatgtgta agactttgct agacagagaa ggggtggggt gagaagatga 90120ggaaggtgca ccttttatgg agaggctttc cttcctcttc acagcaaacc atacctgtac 90180tacattgact tcctttgctt tcccaggtga catctagctc atgctgcaag ctcatcttgt 90240taatcataaa tgctagtaag ttaatattac ccatcatata taacatgact taattttaac 90300aattcaatgc tttatcccca aaagatgact taatggtgac aatttcaatc cccattgtag 90360gatattttgg agacaggcag tcctttcaat gtcatatgtg ggtgcttcct taggcaggtc 90420aggggtgagg tggaaatgag gctgggaccc tgctcactta tatagcaggc atcgttctca 90480ataccaggct tcagggggct ttttggtcta gccattggta tgaactgcct caagaataat 90540cccttcatca ttgtggtcac aattcaggta gaattggaat aatcaccctc tccactctgc 90600attaaaccag gcaaagtttc catctctggg taccattgtc tttcttgatg gacagggtga 90660gtcagaagga aacttactca ctcccattca ttttctgctt attatttcct gcagtgaggt 90720ttccttgtat aataaacagc ttctgtgggt gtttgagctg ctctgaaaag agaacatgct 90780gttcctgtgt gtagaatgcc ttctgaagga agcatcacag tgaacacaga gcagaagctt 90840ggcacacagg tggcagaagt ttgtctgcag tgttctgcat agagcagaga gtcaagccat 90900tttcattctg attgattgga ggcatggtat ggaggtaaat gggtccttgg cctctctcct 90960ggattcaagt ccttcttagc cactgatagg tcatgtgacc atagggaggt tgtttaacct 91020tcctgaacat tcattttctc aagtataaaa tgggggtaat agaatttgcc ttataggctt 91080gcgtataaaa taagaattat tgagagaaag cggggcataa atgtccaata agcggtagct 91140gtctatgaag ccactgttgt tactgggttc ctttctcact aggtggcttc aggtagctga 91200cagaagctct gtgagcctca atttcctcac tggaaaagtg gagtcaatat ctcactgagc 91260tggtgtgagg attaaatgag atgctgtgca ggtgcttagc acagcgtcag gtatgatgtt 91320aatattgata gatgcatttt cttcaccctc acctatcttt ttctgcctgt tggcttatgg 91380ttgaaattcc ttcatgacgg tttccatttc cagagatatc ttgttaacaa gtatatacca 91440ccaaatgaag ctgatttttt tttttttttt tttttttgag acagagtctc gctctgtcgc 91500ccaggctgga atgcagtggc gcgatcttgg ctcactgcaa cctccgcctc ccatgttcaa 91560gcgattctcc tgcctcagcc tcctgagtag ctgggattac tggcatgtgc caccacgtcc 91620agccaatttt tgtattttta gtagagacga ggtttcacca tgttggtcag gctggtctca 91680aactcctgac ctcgtgatcc acctgcctcg gcctcccaaa gtgctgagat tataggtgtg 91740agccaccatg cctggccatg aagctgattt ttttaaacca tcatttaaca ttttctccat 91800aaggtggcaa ggaggaagag catatgggga ctgggtactt tgagagaccc caggacagga 91860gacagggagg ctgagattgg catgttgtct gctgcagtta tttgccagcg acacactctt 91920cccgtccaaa ctaacttctc tgcctcaagg acagggagac tctgcctttc aacctgagag 91980aaaccaggac tctcagcttt aatgaaaatt ggacttaggg tggggcagtg gagacttttc 92040acagctattg tttagctgat gaagcagatg cttctccatc tttggagcct gtcttcatta 92100cctgtggacc tcatctttat caacccagag cacacttgcg tctctctatt ttggctaaac 92160accaaacagc tgaggctggt actgtaaaac tttccctcca aatgcccccc ctcgtcttcc 92220tctattagag atctggatca caaccctcaa aaaccatgtc ccttatgcca cctgagtaga 92280tggtttgatg attaattagg cacagatgtg acactggggg gttctcacaa tggcctgtgg 92340gtcacatgct actttccttt tcattttcat cagcaacagc tgccttaaag ccagttaaga 92400ctgtggtcct agtctcgcac cctggggctc ctgctggggt gggtgagggg aacaccccat 92460taagctgggg gaactggggc tgccaccagg gggcgcgagg ggccttcgcc cgagaagagg 92520ggtgggcagg tgcctccagc ggagaagggc gccgtggccg gaggcacagg tctccccggt 92580gccacttcaa gtgagttcga ggaagtacct gggatctttg atctaacgcg aaaggccttc 92640ccagtgacct cttgagagct gagaacccac tccctccacc tctagtccac ggctttgcca 92700ctccagggcc cgaggttacg tttgctgctg gggatttgac aaacccaaag cctctctggt 92760ttcaccactg gctccttaga atcagacatc tgttctgaat gacacttatg tgagtcaggg 92820gctgaggacg tgatcctcga agtgtggtcc ccagactggc tgtatcagtg tcggcatccc 92880ccaggacctg gttggaaatg catattctca ggccctactc cagacctctt aaatctgaga 92940ctggggctgc ggggagcgcc atctgtgcgc cactatcctt gtgggtggac caggagtcgg 93000ttcgagggtg ctcccactta gaggtcacgc gcggcgtcgg gcgttcctga gaccgtcggg 93060ctccctggct cggtcacgtg ggctcaggca ctactcccct ctaccctcct ctcggtcttt 93120aaaaggaaga aggggcttat cgttaagtcg cttgtgatct tttcagtttc tccagctgct 93180ggctttttgg acacccactc ccccgccagg aggcagttgc aagcgcggag gctgcgagaa 93240ataactgcct cttgaaactt gcagggcgaa gagcaggcgg cgagcgctgg gccggggagg 93300gaccacccga gctgcgacgg gctctggggc tgcggggcag ggctggcgcc cggagcctga 93360gctgcaggag gtgcgctcgc tttcctcaac aggtggcggc ggggcgcgcg ccgggagacc 93420ccccctaatg cgggaaaagc acgtgtccgc attttagaga aggcaaggcc ggtgtgttta 93480tctgcaaggt aagcgcccct tcgctcgagg tgtggtttaa ttgtctcatt ttgtttgaaa 93540tcctgcggtg agaaaccagt cgtgttgaga acaataaaag accaaaaaac gatcaccaaa 93600accaactgtc ctgaaagcta ctggaaagtt ggaaaatgca tgctttgatt aaatgtcttc 93660attcaagaca ctggcaagtt aacttattta gtttgtgccg tgagctctgg gttgattgtg 93720ctaatatgaa taactgaaaa acattttatt tccctatggt tttcctcgat ggacttcccc 93780actatgggtg aaatgacaat ggagttgaat acactttctg attgaacttt gagggcctgg 93840gaagatgtac acgtctcagg caagatgata ggggttttaa aatgtattaa ttggcattcc 93900ttagccatgt cagcaagctg cgttcctcct ttcctgggca gaccaagcta agctctaact 93960ggtctccttt atttgctgaa gaggagtcca acaactgccc tctaacaccc tgcgtgttat 94020tcttattgga aggacaatat taagtcaagt gaatgtcatt tttgtgaaaa aactttgagt 94080ggacttctat ttaggaagat aaggttgatt taattttact cgctgtttaa aaagcaggat 94140tgtgttttgg tgtggtaggc aacattttgg aggacagact ttgccttatt ttgttatatt 94200tctagtattt acatgggcat tccattagaa agttttactt ttgctctaag tttcgtaact 94260cggtgtctag tgaggggaaa catgtttgta atttaaaaag tgaacatgtg aaaggaaagg 94320cttttctgag agtgttgtaa aacaaatgta acgtgactat gaaaagaaca tgattaacat 94380ctttgactcc tattttttct gaagaaaatg tattttgata tgagttctag aagaaggaaa 94440ctataaggat ctgttcatca acaggcatta gagtatacac cgtaggattg cattttacgt 94500tcaagcattt ttttagatga atttctgaaa cattcttatt ttaaaagcca tcagatgctt 94560gttaacactt aagtcttgct caagacatag aagtttctga aatcaattaa catgtttagg 94620acacatttcg tagtgttctg agggatgtga ataaatctaa tcacagttta catttcttaa 94680tgtatttata attcagaaaa ggtagaattt agtagtaaat tcaactcata accatataat 94740taacatttaa tagatattga tatgttcact tttaagaata agaaggaaat tttctataag 94800tgtatgttga acacataata attcaaaatt catgtgataa ttttaggtga tgctttgagt 94860cgttttatag aatataaata tggataaaat ataaaatact gaaggctgaa ctcaaagtgt 94920ttaatgataa gtttttgata atacatctag aaaccttgag aattgtatgc ttgaacgtta 94980gatttcataa ttcagtgtct agcacattgt tttatatgca atagcacttt aaaaaaatta 95040ggctacagca gtataattta catacagtaa aatttagcct ctgtaaatgt acctctatga 95100attctgacag atgcacagtc atgtaaccag caccgcacac atgacacaga acagttccat 95160taccccaaaa gtcccctttg tacctctacc taccccactg cccctgaaaa tcactgatca 95220aaactacata atgattatgt ggttttgctc tttagtacgt ttttacttag acatattttc 95280ctttacttct tttgaaagaa aaacctgttt ttcccttttt ataggatgag tcagtttgtg 95340ctatttttaa ttctagtacc ttgggataaa tcaaggcaaa gacaatgcta tttgcaaatg 95400ggaaacttga gacttggact aagtgttaaa ttcatatagg gctaatagat ttagttctta 95460gcagatttag attctattgt ggtttaagcc tttggttatg gcatatatca ttagttatcc 95520tgaattgaaa tacaaggcca ttaaaagtta tttatatcat attaatagaa tgcatcattc 95580ttttataatc tttgaatttt aaaacttctt tattaaaaaa aaaactactt ttcattatac 95640ctgagattaa gaaagctacc tgaaattgca tattatcaaa tagtgagaag caaaacaggg 95700attgaaaatg acaaattgaa gacatttaaa atgcagagtg attacaattg ctgaaggtaa 95760aatatttatc ttcatagggg cttaggtctg tgtccaactt atttgtagat gtcaggattt 95820ttaaatttct gtgctcatgt cttgaagtct agattttcct gcagggtgga gatgtataac 95880cttttgtaaa ctaatatttt tcactgttta acacagtatt caattcagta tacagttagg 95940agcctgttat tggtaggtac tgctaacata tatatatata aaattgatgt ctttttcctt 96000tttcctttgt tctatgaaaa acagcctgta ttttaaatat gtaacttacc ttgcataccc 96060agttacagtg gtagtaacta ggatatgcag agtggcaagt ttatgaggag ctagcaaact 96120ggatagttgg ccttcctagc tggaattatg acaggtcttg aaaatgaagg gcttttagtg 96180gagaatcttt gtgtgggtgt acttgagaga gggcaggaga gttagggtga cctagaaaga 96240tagattgctg gacttgtata tgtttcctca aagccagact gcagcatttt gttagtaaat 96300tgttgtgtgt tctactgtca aacccaggcc tggaagggga gttgagtgca ttcagcctaa 96360cttctggatt ggctgtgtca tcttgaatcc cttcactcgg aattctctct gaccctgtcc 96420caaatgaata tttgaatttg gtccagttcc tacagagcat ggtctgtggc tgttgttggt 96480gttagggaag agcagaaact tgctgttgag agagaagaca cttgagaaga ctgatgaact 96540ctctcccacc cctgccttcg aggcttggtc ctcctaccct attcaaaccc ttgaaactct 96600ttcctatcca actaaataag cgccaattgg ttactaggag aattagcttt tcctcatttt 96660agaaggaaac agggtttcct tatgtacatg ttcttaagaa ttacatgcaa atcagttatt 96720aatgatgagt tctctggtga ttttggagtg ttttatcttc ctaatattaa attaattgag 96780ggccttaata ttttgttttg aaagaatata tttaaaaagg ctgggtgtgg tggctcacgc 96840ctgtaatctc agcactttgg gaggcctagg tggctggatc acttgagggc aggagttcaa 96900gaccagcctg gccaaataat gaaaccttgt ctctgttaag aatacaaaaa attagctggc 96960catggtggct caagcctgta gtcccagcta ctcaggaggc tgaggcatga gaattgcttg 97020aacctgggag gccgagttta cagtgagccg cgatcatgcc actgcattcc agcctgggca 97080acaaagcaag actctatctc taaataaata aataaataaa taaataagaa tacatttaaa 97140gataataatt ggccaggtgt ggtggttcat gcctgtgatc acagcacttt gggaggccga 97200ggtgggagga ttgcttgagg caaggagttc aagatcaatc tgggcaacac agtgagaccc 97260tatctctaca aaaatttaaa aatcagctgg gcatgatggt gcatgccttt agtcccagct 97320acttgggggg ctgagtttgg aggatccctt gagcccagga gatcaaggct gcagtaggcc 97380atgatcttgc cactacactc tagcctgagt tacagagcta gagtataacc cccacccccc 97440aaaaaagcta ataattgtca aacagctact tatgcacatc aaggatgctt gttgcttaag 97500aaatcttttt aaatcttttc catgaaattc cttctagttg ctgctttgtg agcgtgaatt 97560ttttacttct gcaggacaca caaatgtgga gcatttgaac tgaatgcttg ggaaagtgtg 97620atgggcaggt ggaagaagaa tagggatgag gacttatcct ctattcttat cctcctagac 97680ttatcctcct agtctgcaag cttgagaata tggcatcagg aatatgtggc attttgtcca 97740cacacacagt gttggcaggc taccagcagc ccagctatct ggactagggg tgatggattt 97800ctgtggacag aagtcaaaaa gtaaaattag gaggcaaaaa tcttcagggt ggccataaag 97860acattgtaac ttgtctggaa attccaacca acactaaatg tgtatccagt gatataccaa 97920tagactggct tcatcttctt ggatgtgtaa taatacctta cagaatgctt tctttttttt 97980tttctttttc tttttcttta ttttttttga aatgaagttt tgctcttgtt gcccaggctg 98040gagtgtaatg gcacaatctc agctcactgc aacctccacc tcccaggttc aagcgattgt 98100cctgcctcat cctcccgagt agctgggatt acaggcatgt gccaccatgc ccggctaatt 98160ttgtattttt agtagagacg gggtttctcc atgttggtta ggctggtctc aaactcccga 98220cctcaggtga tctgcccacc ttggcctccc aaagtgctgg ggttacaggc gtgagccact 98280gcgcccggcc tcagaatcct ttcacagaca tcatctcatt tcaccctcag agcaccgtga 98340aaaggtacag caccaaatag gtacctgatt ctactgaaga agatgtggca gctcagggag 98400tttgtggatt tgtctaagat tgcctggctt tcaggcagag ctggggctag aatgaatgtt 98460ctgctctatc cattgataga atatacataa gaacaggctt gatggtggct gacctttttt 98520tttttttttt ttttgagaca gagttttgct cttgtcacct aggttggagt gcagtggcgt 98580gatctcggct caccgcaacc tccacctcct gggttcaagc gattctcctg cctcagcctt 98640ctgagtagct gggtttacag gcaagcgctg ccacacccgg ctaattttgt atttttagta 98700gagactgggt ttctccatgt tggccaggct ggtcccgaac tcctgatttc aggtgatctg 98760cccaccttgg cctctcaaag tgctgggatt acaggcatga gccacccgcg cccgggtgac 98820tgatttctta ttaactagat ttacaggtgc tttgataaaa accagtctag tcttggctgg 98880cacggtggct catgcctgta atcccagcac tttgggagcc caaggcgggc gggtcacgag 98940gtcaagagat caagaccatc ctggctaaca tggtgaaacc ccgtctctac taaaaaatag 99000aaaaaattag ctgggcatgg tggcgggcac ctgtagtccc agctacttga gaggctgagg 99060caggagaatg gctgaacccg ggaggtggag cttgcagtga gccaagattg caccactgca 99120ctccagcctg ggcaacagag caagactcca tctcaaaaaa aaaaaaaaaa aagtgtagtc 99180tttttggagt gtttttctgc catttctagg gccaaacttt ttcttgtcca tgaatcattg 99240tcaaaattgg gaattttaaa tactactttt ttcttttaat tcaaaagcca tagtatgttt 99300cccagccagt acattagaac accatgcacg atcccatgtg tacaaaaagc tttctggctg 99360aattcagatg tgacctgaga gggccaaata caggggtgtg tgctgggaga gagagagagg 99420tctctggaca gaaaacaaag cctgttcacc acccaggata tggaccaact attttaggtt 99480atggtgacta aagaaaattg acatgcaaat aaatgaataa ttcttagaat caggatgtct 99540gggtactggt tctttggttg gccaggtgaa attccatgcc aggcccaaca attaaactct 99600ttagagacaa ttttttcctg ttgtaccaga acattgtact gaggccatgt ttgaacattc 99660aatcgatgtg ttgggaaaac tctgccctac aatgttaaag aaattaaatc ttttggggag 99720tctttccttt gaccagttta tatctctgtt ttagaggagg gcttctcaac cagaatgggt 99780ttgttgactt atttttacag acctctggta gaaaggaggt ctttttttgc tacctgttct 99840cctgtctcag agaactatta caatggtgta agttcatcat ttcttcccct tattatggct 99900ctgcttagga agaaaaactc tttgcattgg ctaccaagta cctaactatt caagatgcca 99960ctgacaaaga gttaatctgt gaatcatgtg aatctgatat atctgaaata tatccaaaca 100020aaaagcacct agccttttaa tgactctcca gaagtcagtt ctctaacttt aattatcatc 100080cttctgggga tatgtggaaa ttctacagaa gttgattggt gatatgttga gatgtgagat 100140ctgtattttc taagcaaagt tgccatgcac ctgattgatt ggctaggtgt atcctggcat 100200ttgtcatttg ttggtggggt ctgatagttg gtttcaccac tgctgggtac ccagagtcat 100260cacatccata gagacagaat gtaggctggt ggttgccagg ggctggggga agggaggagt 100320ggggaatttg tttaacagag agttttagtt ttgcaagatg aaatgagttc tagagattgg 100380ttgcacaata atgtgaatat ccttaacact actgaacttt atacttagaa atggctaaga 100440tggtaagttt tatgttacat gtattttaac acaattaaaa aagaaaaaaa aaaaaacaac 100500ttcaggccag gcacggtgac tcacacctgt aatcccagca ctttgggagg ctaaggcggg 100560cagatcactt gaggtcagga gttcaagacc agcctggcca acatggtgaa accccatctc 100620tactaaaaat acaaaaatta gcctggccta attgtgcatg cttataatcc cagctaattg 100680tgaggctgag gcaggggaat cgcctcaaac cctggaggtg gaggttgcaa tgagccgaga 100740tcacaccact gcactctcca gcctgggtga cagagtgaga tttcatttca aaacaaaaaa 100800ccactttaga aactgctagt tttggcaata gttatcacta tatgttttat cctgcatatt 100860ttctgttaag aataaggaat tgtttatgtt gatcaggaat ctaagtaatt aaaatacaaa 100920attctggctg gtggctctcg cttgtaatcc cagcactttg ggaggccaag gcgggtggat 100980catttgaggt cggaagttca agaccaggct ggtcaacatg gtgaaacccc atctctacta 101040aaagtacaaa aaattagctg ggcatggtgg taggcacctg taatcccagc tactagggag 101100gctgaggcag gagaagcact tgaagtcaag aggcggaggt tgcagtgagc caagattgta 101160ccactgcact ccagcctggg tgacacagcg aaactccatg taaaaaaaaa atgaaatata 101220aaattccata ctcattatta attacatata gtattaaaat aaaacccaaa caccaaacct 101280tccttgatcc tatatccttc tccagctacc attctctctc ctctccttgg tccaaatttt 101340tgatttacaa tgttggttgg aagtggtacc actttggtgt tagttcctta tcattttacc 101400tggtctgtcc tgcctcttcc tggtacatta gctccctgaa ggcagggtgt atgtcccaga 101460actccttgaa gtcccttttc tcagcatact accatgccta ctgcagcacc ccccatcttt 101520aatgtccttg acttggtgaa atattacatt ttgaacacat ttcctcactt ccttatgaca 101580aatattgatt gagtttcagt gcaaggtgag taagaaatgg tacttgcttt caaggagcta 101640aaaatctgaa tttccttttt tttttctttt tctttttctt tttttttttt ttttgagaca 101700gagtctcact ctgtcacctg ggctggagtg cagtggcacg atctcagctt aatgcagcct 101760ccgcctccca gattcagtga ttctcatgtc ttagcctctc gagtagctgg gactacaggc 101820atgcaccacc acgcctggct aacttttgta tttttagtga agatggtgtt tcaccatctt 101880ggccaggctg gcctcaaact cttgacctca tgtgatccac ccacctcggc ctcccaaagt 101940gctgagatta caggcattga ctttacttct tactctccta tgcacctcta tcattttgaa 102000gaagggttca aggtagttct gataagcagg attaggtttg tatgtaagtg attaaagggg 102060tgctatgagc aaaaaaagtg tgaaggtata acaagccaac cacctcacaa tgcagtttgc 102120atgtttctta atggacatag caggttttct gtaagaaaac agcaggagat tcgtgtggaa 102180tgatgggttg aggcaacata gtggcatccc ttgaatgctc gaagaatgtg acttagagtt 102240tggtgggaag cagagagctg ggttttaaga acatgaatct gacaactcta tggatctgga 102300ggagaagcta actggggacg aggagcagta agaagcctgt tacagatgca ctgataagaa 102360gtaatgagag ctggccgggc acagtggctc acgcctgtaa tcccagcact ttgggaggcc 102420gaggcgggca aatcacaagg tcaggatttc aagacgagcc tggccaacat ggtgaaacgc 102480cgtctctact aaaaatacaa aaagttagct gggcgtggtg gcgggcgcct ataatcccag 102540ctactcggga tgctgaggca gaagaatcgc ttgaacctgg aaggtggagg ttgcagtgag 102600ccgagattgc gccactgcac tccagcctgg gtgacagtgc gagactccgt ctcaaaaaaa 102660aaaaaaaaaa gtaatgcgat aatgagagct tacttcaaga tggcagcaaa agacagtgga 102720aaaaaggcat tgggaaaaaa agccaatgtg ccttgatgag taaagttaac tgagtcaagg 102780ggagaagtca aaggtaacta tgatgggctt tttctattaa cacaaatagg aaatgagtgg 102840ttttgggaaa gaaagtgatg aattacccct cagatattgt attaattgtc tattactgtg 102900gccgggcatg gtagctcatg cctgtaatcc cagcactttg ggaggccgaa acaggcagat 102960cacttgaggt caggagttcg agaccagcct ggccaacacg gtgaaaccct gtctctacta 103020aaaatacaaa aattagtgtg gtggtgtatg cctgtaatcc cagctactca ggaggctgag 103080acatgataat tgcttgaacc tgggaggcag agattgcagt gagctgatat ggcgccattg 103140cactccagcc taggcaacaa gagtgaaact ccatctcaaa aaaaaagatt tgcctgtaat 103200cagccagcac ccccagcctt gtgctcactt tacatacaaa aattctgttt tttagagcat 103260aaattgaagg gcacattcaa aactgatacg taggccaggc atggtgactt atgcctgtaa 103320tcccagcact ttgggagacc gaggcaggtg gatcactcga gatcaggagt ttgagaccag 103380cctggccaac gtggtgaaac cccatcccta ctaaaaaata caacaaatta gccagtcaca 103440gtggtgcgca cccatagtct cagctactcg tgaggctgag gcaggagaat cactagaacc 103500tgggaggcag gaggttgcag tgagccgaga tcatgccact gcactccagc ctgggtgaca 103560gagtgagacc ttgtctcaaa aacaaagaca aaaccaaaac aaaacaaaac tgagaagcaa 103620cagattgata agtgacacag ttacactggt cagtctcttc agctaatacc cattgttttt 103680tattattgga gattcataat gtgttttctt tcttttaaaa acttttttcg gaaatggtaa 103740tttctctctt tttttttttt tttttttttt tttgagacag ggtctcactc tatcacccag 103800gctggagcgc ggtggcacaa tctctgctca ctacaacctc tgcctcctgg gcttgagcaa 103860tcacacctca gcctcttgag tagctgggac aacaggcaca tgccaccatt cctggctaat 103920ttttagtaga gacggggttt caccatgttg cccaggctgg tctcgaactc ctgacctcaa 103980gtaatctgcc cacctcagcc tcccaaagta ttgggattac aggcgtgagc cactacgctt 104040ggcctcatag cgtattttaa tattggttga gactagcctt gctcattgat cttctcttag 104100cgtttacttg gttattcttg cttatttttc cataagaact ttcattttta tttaatcctg 104160tgttttttgg ttttaaagac tattttataa taaattttcg tgattaaact cttgtgctta 104220aactcttgat taaacaaaca agcaatgaag agatgaatga agcagaaaat gtgagtttca 104280tgcctcacat tcccactcct ctgaggttaa tattttcatg tatatttttc aggatgtatt 104340tgtaatctca tacaaacgta tgtatttttt taatgaaaat atttaaattt tcatagttaa 104400cagctgtagc tctaacttgg caatatcttc tgtgtttctt tacagccatt atacttgccc 104460acgaatcttt gagaacatta taatgacctt tgtgcctctt cttgcaaggt gttttctcag 104520ctgttatctc aagacatgga tataaaaaac tcaccatcta gccttaattc tccttcctcc 104580tacaactgca gtcaatccat cttacccctg gagcacggct ccatatacat accttcctcc 104640tatgtagaca gccaccatga atatccagcc atgacattct atagccctgc tgtgatgaat 104700tacagcattc ccagcaatgt cactaacttg gaaggtgggc ctggtcggca gaccacaagc 104760ccaaatgtgt tgtggccaac acctgggcac ctttctcctt tagtggtcca tcgccagtta 104820tcacatctgt atgcggaacc tcaaaagagt ccctggtgtg aagcaagatc gctagaacac 104880accttacctg taaacaggta agtccagtct tcattctgaa ttatagttgc tagccatttc 104940tcaaatcact ttatggttga gtgagaagga aataatatgt tagacaaggt ctttattgta 105000ttaattacat agtttactta cagcacccaa aacacaggat gccctgttct attctgatat 105060tttagttctc attaaaaact ggtatgtgta catcagtgtt gtggggagaa tttgctatca 105120tgactattgt ctttatacag taaatactga acttaagtca ctccttttct ttttttgaga 105180cagggtctcg ctctgtcact cagactggag tataatggca cgattgcggc tcactgcaac 105240cttcacctcc tgggttcaag caattctcgt gccttagtct cccgagtagc tgggattaca 105300ggcgcgtgcc accacgccca gctcattttt taaattttta gtagagacag ggtttcacca 105360tgttggctag gctggtcttg aactcctgac ctcaaatgat ccacctgcct tggcctccca 105420aagtgctggg attacagacg tgatgaacac tgtgcctggt ctgaacttaa gtcactctta 105480atggagttat ttggatttga aaaatgaatt tttactttac tttcagtttc aaagtcttct 105540tatagtgaaa ccacaattta atgttcatga caaattgttt ccaggataaa agtaactgtg 105600atagtattac aacttaaatg aaattctaga catgcgaagc atgaaaagat agatgattgg 105660tataagcttt ttaaccatga actaaaataa taacattata taaagattgg tggaaactat 105720tgaagtttag gcttcagttg acattccctg aagttaaaaa ggatatgtgt actctttaaa 105780tgcaaggtaa cataatggat tatttccatc taattattaa tatttctaat gataatcata 105840ggtatgaagg gaatggatag tataatgaga aaggagaggg ggagataaaa atctaaaagt 105900actaagggca tgttggatat tgaaattcac tactttcaaa tattatcata aaactttgag 105960acagtaacat tgcaccatta tttttcttct tttaaaaaca ttttactcat tggtaaagag 106020aatataaaca ttgtggataa cttttttaaa gtaatggttt gttttttttt tctccttcct 106080cctttaaagg aagacatatt ttgtttctga gcatgaatta taatcaaagt tctgctaatt 106140tttgggcaaa ttaatccatt atataattac cttcatttat aaatcaataa tacctttacc 106200attccctttc caaaagaacc atgcctggca acatcaggaa ctagccagat gtgttttgga 106260ggctgcctgg ggatcccttg ttagactttt cgttccttta tgaacctctt gcctgtggtc 106320cagcattgag cctctgcttc cttccaagcc tttccaggcc aggcacttgc ttgttctctc 106380tcttctcttc tctcttcttt tttctctctc cctttctctt ctcttccccc tttttcttgt 106440ctcacattca tctcaaggta acttaaagtc catttgttat tcctcttaaa gttattttta 106500ttttattttt ttgagatgga gtctcactct gttgcccagg ctggagtgca gtggcacagt 106560cttggctcac tgcaacctct gcctcccggg ttcaagcaat ctcctgcttc atcctccaaa 106620gtagctggga ttacaggtgt gcaccaccat gtctggctaa tttttgtatt tttagtagag 106680atagggtttc accttgttgg ccaagctggt ctcgaacttc tggcctcagg tgatacgccc 106740accttggctc cccaatgtgc taggattaca ggcatgaacc attgcgccca acctgaaagt 106800tattttaaat ctagaccttt atctgaaatt gcagagtgtg agatgtttgt tctccattta 106860aatgggaact tcaaatgtct gaagggctgc ttagcaatgc tgttgggaat gactgatgtt 106920tggaagtggt tgaatgcctt cacacccatc catgcagcat tcgtgaactc tagtaactac 106980agaagaccaa tgcatatcct gcctgtggtt cagacctgtg ggtaagattt gatctggcca 107040ctcctttcat tacacttaga gatgtagctc ccaccccatg gctatgactg gtcttcggca 107100gtgacaaatg ctcatcagca tcacgtggat gggcataaac tcacctaccc actttcaaac 107160attagtcatt ccccacagcg tggctctttg tagatatgat atcagtatca aaagctttgc 107220tgtatcagat ttccgggaat atatttacca ggaaccctgg aggaaaaaga gattaaatta 107280ggcaatgttc atgctatttt tttttcctag aaagcccttc ctttcccttt tatgctctgt 107340tcaatggata ttttctttgc tccctagaga gacactgaaa aggaaggtta gtgggaaccg 107400ttgcgccagc cctgttactg gtccaggttc aaagagggat gctcacttct gcgctgtctg 107460cagcgattac gcatcgggat atcactatgg agtctggtcg tgtgaaggat gtaaggcctt 107520ttttaaaaga agcattcaag gtacaagaga attgttaact gcttctttag tttcctactt 107580ttgatttcaa acaattttgc agagatgact tggcagaaat gtcactactg gcctgtttgg 107640cacacaaagt atttgatgag cagttcagag gatcatgtgt gtttggaagt gggttgggtg 107700gtggggtgga attgcagatt tctaccccag aaccccaaga ttatacagcc aactcgaatg 107760ggtcttaccc ctcgttcacc cacatgggtg ttggatagaa gacatcgagt tacaaccttg 107820tgaagatgtc tcttggaaaa aatgtgctca caaggagttg caaagattgt ttctttcttt 107880tacttaaatt taatatatag catgcttaac agtcatgatg gtgggctggc tcctgaggaa 107940gaaagaataa acacattttt tggaaatggt cagaaatcag gaattcagct acagtggact 108000ttgagaattg atctagacac atttcttccc ctaggctagg agggtctcag ttcacaatcc 108060ccttgttttc tgggctgtgt ttagattatt tccctaactt tctctaaacg ccttctggat 108120tttttttttt aaatcaactt gttgatgaaa agaatcaaac tctgtaaaat atttgaagag 108180atttattctg agccaaatat gagtgacaaa tggcctgtga catagccctc aggagatctg 108240agaacatgtg cccaaggtgg tcaggccaca acttggtctt atacatttta gggagacata 108300aggcattaat caatgcatgt aagatgtaca ttgattcagc ctgaaaaggc aggacacctg 108360aaagcagggg cttccaagtc acaggcagtt caaagatttt ctgattggca attgattgaa 108420agaattatta tcagtaggaa gcaatgattg ggttacaata agggattgtg gagaccaagg 108480ttttatcatg cagatgaagc ctccaggtag caggcttcag agagaataga ttgtaaatat 108540ttcttagggg tcttaaaggg tctgttctat cagtgattcc aaaaggggag ggagggtata 108600atgaaccatg tctgtctccc ttgttccatc atggcctaaa cttatttttc aggttaactt 108660tgtaatgccc ttggccaaga ggagggaccc attcagatgg ttgaggggcc ttagaatttt 108720attttttggt ttataaactt caagttgtgc acccctgatt tcaaggctgg tcagctcatc 108780tccctgcatg tgtctttgct acactccttc tctcgtacca gccctgattt gctgaagtca 108840ctttcttgct tactcttgtt ttctctattt gccccataac ctgtccctca actgctccct 108900cccaggcaac accctatgtt tccatctgaa agctcccttc ctttttctat caaagcccca 108960atgctttgtt ctttgccagt taagaaaagc aacgttgaga gaattcatag tgtgtaaatg 109020gcaaatagca atttactaaa ttaactcacc cattgataac tctaagagga tgttttacct 109080taagcagaga aatactgata gaatccagga tatggtgagg agtgaaatgt tggtagtcac 109140cttcctacct gtcccctgaa attcaccctg tatgaatggc agcctctttg tcctggattt 109200tataattact agctctgcga cttcacctcc tagcctgttt cctcctctgt gaaatggaga 109260tactcatagg gattttctaa agatgaaata aggttgatta tatgaaaaca tattcagtgc 109320tcaaatattt tatttgtgac aatcttaaca gtagattata aggccaagtc catttcctgg 109380ctatatgata agaacaatat tgattttctg aaattctgaa ctgaattctt gatacgatga 109440ctattttgta tcttgctgag tttctaggat tttacccctt aagaacgttt ggacctatta 109500ctactaacca tatcttttaa aaagagatcc ttcttttttt tttttgcttt tggggaaaca 109560ttggtctgct tgaaacatct ttgacccctg agactacagc taataacaat tgaaagtaaa 109620tttcctttgc ttctctatgt tgtttcttcc ttcctgctgc atcagacagg aatgtcaaat 109680tctaaatgtg caaagaggaa agagttaaag ctgttacagt tgtacagttg tagtgcctaa 109740atgatccttt ctttgcatgc ttcctgtctt tgatataagt gcattacagt aactgaaagt 109800ggccacttat ttttaaaatt gtctcaaata ggccaggatg gtacagtatt gagaaattcc 109860ttgcatgtaa cttttttttt tttttttttt tttttggaga tggagtctca ttctgtcacc 109920caggctggag tgcagtggca caatctcggc tcactgcaag ctctgcctcc cagattcaca 109980ccattctcct gcctcagact cccaagtagc tgggattaca ggagctggcc accacaccct 110040gctaattttt tgtattttta gtagagacag ggtttcggca tgttagccag gatggtctcg 110100atctcctgac cttgtgatcc gcccgcctcg gcctcccaaa gtgctgggat tacaggtgtg 110160agccaccgca cctggcccat tcttatgttt tttataattt taaacttgtc ttgctaactt 110220gatttataag ctaattgacc atatcttagt catgtacctg tccccttcac tgtacaaatg 110280cactggaagc tgtgttgtgc ttgcttttcc attgatactt tgttggcttc ttcacacaat 110340gagttgccat cagagtgata agtgctgttg tttctctact gggttatgga gcacagagga 110400aggaggacat agggagaagg acctcatcac ttcatctggt ccagatgaca gcatggctta 110460ttttttgagc ttatccttta ctttttgttc tctttcctat tggtgttcat ttaacaaata 110520tttattgagc attgtaccag gctctacgga tgcagtggtg aacaagacaa tagattatag 110580attccatgag ggcaaggatt tttgtccatt ttgttcacta gtggcactta ccaattcctt 110640gaatatgatt tttcaaaatt aattgggctt atacacagag ttctgtatca tttttcactt 110700aatattgtcg tataagcatt tctgttgtta aatttccaga gacccttttc acaactgcac 110760aactgtgtaa tattctatct tatgatcaga tttaatttat ttaactcttt attattgaag 110820gccctatcaa ttattttcat tgttttaatg ctatggttac tttatttgtt catgaagttt 110880tgaaaaaaat aagtttctct ggatatgttt ttagaacaaa tctgtggggt cagagtgcat 110940taatgtttaa agttcttgac agatgttttc aagtgttcaa gtcttaagaa ggttgtacag 111000acttgctctt ttaccagcag tgtgagtgtc gctttttcca acctcttggt agcattgact 111060cttatcaaaa agaaaaaaac cttgctacat tgatacgtga tgtatagtat cttttggttt 111120caatttgctt ctctttatta gtgaggtaaa tgttttctca taaatctatc tgccatttgt 111180attttctctt ttatcttctt tattcagaga ttttgcccgt ttttatattg ggttctggca 111240tttgcttgat aaatttattg tgtgctttat atattaacct attattacat gtatgacaaa 111300tattttttcc acttgactct gattttgata tgcagaaata gttaatcttt aaatagtcaa 111360atattaccaa ctttgatagt tttgtgtata gtttttaagc ctaaaaaaag tcctttcata 111420cccaggcatt atataaactt ttgcgcatat tttgttattt aatagtttgt ttttacattt 111480tattatttaa ttcaaaagaa atttattttg gcatactgaa tgaaataacc aaatgtattt 111540atctctcgtt aattttctcc acatcatttt acttaataaa tccattcatt tttcattgat 111600ttaaaatatg ggagccaatt tttaaaagtt gagtttgaga tataatatgc atacaataaa 111660atttacctat tttcattact ggtaatagta agtacataga aaaccacaga ataatataac 111720actgttaatt gtggtttgta acctcatatt ttgagtagaa agtctaaagg aagaaccaat 111780gaaaaacaat aactacaact ttttttttct ttttttttga gacagagtct cactctgtca 111840tccaggctgg agtgtaatgg tgcaatctcg gctcactgaa acctccgact cccaggttca 111900agggattctc ctgcctcagc ctcccaagta gctgggatta cagacaccca ccaccacgcc 111960tggctaattt ttgtattttt agtagagatg gggtttcacc atattggcca ggctggtctc 112020gaactcctga cctcaggaga tcagcccacc tcagacacat gtagattgaa aataaaggga 112080tggaaaaata tttcatgcaa atggaaacca aaaaagagca ggagtggcta tacttagacc 112140agaccaaata gagttcaaga caaaaactat aaaaagagac aaaaaaggtc actaataata 112200aagatgtcaa ttcagcaaga gaatataaca attgtaaata tgtctggagc acacagatat 112260ataaagcaaa tattattaga gctaaagaga gagacagact gatatggtaa tagctggaca 112320ctttaacact ccactttcag catcgaacag atcatccaga cacaaaatca acaaagaaat 112380gtcagattta atctgcacta aagaccaaat ggacctaata gatatttaca gaatatttca 112440tccagtggct gcaacataca cattcttttc ctcagcacat ggatcattct gaaggatata 112500ccatatatta ggccacaaga caagtgttaa aacattcaaa aaaactggaa tcaaatcaag 112560caccttcttt gaccacaatg gaataaaact agaaatcaat aaagaatttt ggaaactata 112620caaacatgga aattaaacca tatactcctg aacaaccagt gtgtcaatga agaaattaag 112680aaggaaatta aaaatttctt gaaacaaatg gtaatggaaa caacatacca aaacctatag 112740gatacagtga aagcagtact aagaggaaag tttatagctt aagtgcctac atctaaaaag 112800tagaaaatct tgaagtaaac aacttaatga tgtatcttaa agaactagaa aagcaagagc 112860aagccaaacc caaaattaat agaagaaaag aaatattcat aaaaagatca aagcagaaat 112920aaatgaaatt gaaaccaaga aaacaacaca aaagattgac aaaatatgaa ggtttttttg 112980agaaaataaa cctgacagat ctttagccag actaattttt ttaaaaaaaa gatagaagag 113040tcgaataaaa tcagatgaaa aaggagatgt tacaactgat accgcagaaa tctaaaggat 113100cattataggc tattataagc aactatatga taataatttg gaaaacctag aagaaatgga 113160taaattccta gaccacatac atactgttaa gattgaacta tgaagaaatc cgaaacctga 113220acataccagt aacaagtaac aagattgaag ctgttataaa aagcctccca gcaagctggg 113280cacaatggct catacctata atcccagcac tttgggaagc caaggcagga ggatcacctt 113340aacccaggag ttcaagatta gcctggacaa cacacagaga tccctatctc tacaaaaaaa 113400aaaaaaatta caaattagcc aggtgtggtg gtatgcatct gtagtcccag ctcttcagga 113460ggctgaggtg ggaggatagc ttgggaccgg gaagtcaaga ctgtggtaag acaagattgc 113520accactgcat tctagcctgg gtgatggagt gtgatcaggt ctcaaaaaaa aaaaaaaaaa 113580gtctctcagc aaagaaaagc caggactgat ggcttcatcc agaattttac caaacattta 113640aagaagaact aatgccaatc ctattcaaac aattctgaaa aatagagaag gaggagggaa 113700taatttcaaa atcattccgt gagaccagta ttaccgtgat accagaacca aagaaacatc 113760aaaagaatat gacagaccaa tatccccaat gaatattgat gtaaaaatcc tcaataaaat 113820acaaaccaaa tgcaacaaca cgttaaaaag attattcatc ataaccaggt ggaatttatc 113880ccagggatgc aaggatggtt caacatatgc aaattaattt gatgcaccat atcgacagaa 113940tgaaggtgga aaaccatata atttcaattg atgctgaaaa ggcatttgat aaaattcaac 114000atcccttcat gataaaaacc cttaaaaaac tgggtataga cagaatatac ctcagcccaa 114060taacagacat ataacagacc cacagctagt atcacactta atggagaaaa actgaaagcc 114120tttcctctat atggaacatg acgaggatgc ccactttcac cactgttatt caacatagta 114180ctggaagtcc tagctagagc aatcagaaaa gagaaataaa gggcatctaa attggaaagg 114240aagaagtcta attatcctag tttgctgatg atcttatatt tggaaaaatt gaaaaattcc 114300accaaaaaac tattagatct aataaattca gtaaagttgc aggatcagta gcatttctat 114360atgccaacag caaacaatct gaaaaaaaaa tctaaagtga tctcatttac agtagctaca 114420aataaaatac ctgggaatta accaaataag tgaaagttct ctacaatgaa aactataaaa 114480cactggtgaa agaaattgaa gaggacaaaa aaaatggaaa gatattccat gttcatggaa 114540tggaggaatt aatatgtcca tactacccaa agcaatctac agattcagtg caattttatc 114600aaaataccaa tgatattttc acagaaatag aaaaaacaac cctaaaattt gtatggaacc 114660acaaaagatc cagaataacc aaagctattc tgagcaaaaa tatcaaaact gtggaagaat 114720cacattacct gactataaat tataccatag agctatagca accaaaacaa cgtggtacta 114780gcctaaaaca gacataggga tcaatggaac agaatagaga acccagaaac aaatccatac 114840atctacagtt aactcatttt tgaaaatagt ctcttcaata aatggtgctg ggaaaactgg 114900atatccatgt acagaagaat aaaactagat ccctatctct caccatatac aaaaatcaag 114960tccagatgga tcagtgactt aaatctaagg cctcaaacta tgaaactact aaaagaaaac 115020acggggaaac tctccaggac attgggtggg gcaaatattt cttgagtaat aataccacac 115080aagcacaggc aaccaaagta aaagtggaca aatggaatca catcaagtta aaaaactgct 115140tgcatggcaa aggaacaatc aatgaagtga agagacaaca cacagaatgg gagaaaatat 115200ctgcaaacat ctgacaaggt attaacaatc agaatataga aggagctcaa acaactctac 115260aaaaaaactt aaaaatccaa tttaaaaatg ggcaaaagag ctgagtaaac atttctcaaa 115320agaagatgta caaatggcaa atgggtatat gaaaaggagt tcaacatcat taattatcag 115380agaaatgcaa atcaaaacta caatgagata ccatcttacc ccaaagtagc ttatatccaa 115440aagatgggca ataacaaatg ctagtgagga tgtagagaaa agggaaccct ggtatactgt 115500tggtcagatt gtaaattagt acaactacta tggagaacag tttgaagttt cctcaaaaaa 115560ctacaaatag agctaccata tgatccagca atcccactgc tgggtatgca cccaaaagaa 115620aggaaatcag tatatcgaag agatgtctgc agtcccatgt ttgttgcagc gctgttcaca 115680atagccaaga tttggaagca acctaagtgt ccatccacag gtgaatatag ataaagaaaa 115740tgtggaacat atacaccact attcggccat aaaatgaatg agatcctgtc acttgcaaca 115800acacagatgc aactggaggt catgttaagt gaaataacca gacacacaaa gacaaacctc 115860ccatgttctc acttatttgt gggagctaaa aataaaaaca attgcagtgc ctcatgcctg 115920taatcctagc actttgggag gtcgaggcag gcagattgcc tgagctcagg agttcgagac 115980cagcttgggc aacacggtga aaccctgtct ctactaaaat acaaaaaatt agctgggtgt 116040ggcggcatgc gcctgtagtc ccagctactc gggaggctga ggcaggagaa ttgcttgaac 116100ccgggaggtg gaggttgcag tgagtcgaga tcatgccact gaactccagc ctgggtgaca 116160gagagagact ccgtctccaa aaaagaaaaa gaaagaaaac aattgaactt gtggggataa 116220agtagcaggt tggttgccag aggctaggaa gggtagtggg agtggggaaa gtgggagtcc 116280cagctacatg ggaggctgag atgggaggat tgcttaagct caggaggtgg aggttgcagt 116340gagttgagat cacaccactg caactccagc ctgggcaaca gagggagacc ctgtctcgga 116400aaaaaaaaaa gatgataaat caaagtattt taataaaatt gggccatact agagatgtta 116460ttgtttgaaa tcaaatatat gaagtatagt taataatata agtgtaatag aagaaaagga 116520gccttagaaa gcttgaaaaa catcgttgta cttcatatac atcttctttg cttacattat 116580aatggacaat gctgcaacaa acatggaagt gcagatatct ctttgcaaca tgagagattc 116640atgtagatct aagagactgt gaagactgtt tcaatattgg agttacaatt agctttttaa 116700ttacctcttc tggccagctg tggtggctca cgcctgtaat cccagcactt tgggagacca 116760aggcgggtgg atcacctgag gtcaggaatt cgagaccaga ctggccaaca cggccaaacc 116820ccgtctctac taaaaataca aaaattagct gggcgtggtg gtgggtgcct gaatcccagc 116880tatttgggag gctgaggcag gagtatcact tgaacccggg gggcagaggt tgcagtgagt 116940cgagatcgtg ccactgcgct ctaacctagg caacagagga aggttctgtc tcaaaaaaaa 117000aaaaattacc ttttcattgt ttgctaatgt gtagaattct gcatgtaaca tgtccagttt 117060aaagaatgat tatagagcaa atccctgtgt aaccagcgct caaacaatga aatagaatat 117120aatggcagcc cagaatccct ttgggtggtc cctcctgcca cacctacttc cctccctgca 117180gaggtgggac agtcctcctt tctgccttcc ttgctgtgta tggttttacc acctacacgt 117240gcatccctaa acgatgcagg ttgattttct ctgtttttga actttatgtg ttcatgtctt 117300ctacatatat tttgtgactt ttttctttta tgacttgctc atttcattta tcattgagat 117360tcatttattc tccgcactga aattctggtt cattataggc ttaatgtaag atgttgaggc 117420catattgttt attagaaagg cactaaaatg ccctattcac tttcactttt gcttctcatc 117480tattttatta taatttctat ttcttaaccc tttctcaaac ccatgtagct gtcctcatcc 117540tccctaccaa caccccatcc agacatctct catttgcaca actacagcag gctgtcatct 117600ggtctcctgg cctcatcagt tttgctgcct ctgattggtt cttcgtactg catctgaaat 117660aatttttgaa atataaatat tcttagctct tccttgcata agagaattaa aagtaccatt 117720gtcttagaga ttgctatata actaacaagt tcaaactctt aggattttac tacaatgggc 117780cttattttct gtacttctgt gtctttgagt tgtttagagt agctctactt cataaacttg 117840gttctgggct tttgtgggag gtcaggtctg ttccacatgt ccccacgttc ttcttggacc 117900agtggctacc tcacaggagc tcaagaggcc aagccaaact gttggagcag cacatctatt 117960gatatatcat tggctataaa aagtcctgtg gtcaagccca acatcaactg gtagggaagt 118020gtttgctctc tgcgcactct agtacactgc agggtcgcaa ggctgaggga gagaatgaag 118080aattgagaac ggtaatccac cacaactctt gtcaatagca gtactttctg tcattattta 118140gattgctaat ttctttattt gttccttttg ttattttatt tgactatgaa ttcccataaa 118200aatattgtat taaacccgaa agagggatat atgtaaaaga atataagaag ttgaatttga 118260tgacttgatt tacaactctt gagttctgtg acttggagca aatcaattta atgttagtct 118320tattttccac atccaaagga tatattttta tatctctctt ttgagaattc taagaatatg 118380cagagaataa catattagta aaaaaccagg atattgaaat gttcctaggt ctcctttact 118440cattaacaag gtgacaatgt agcttgactt tggctttgta cctgtactgg tcattaagaa 118500gatgtcccct atctctcagc tggaaagtgt tatcagtgtt gttgaccagg aagagattta 118560actaagagat catagcaata atcttttttt ccctcccact ctgctatagg acataatgat 118620tatatttgtc cagctacaaa tcagtgtaca atcgataaaa accggcgcaa gagctgccag 118680gcctgccgac ttcggaagtg ttacgaagtg ggaatggtga agtgtggtga gtgcttgctt 118740cccttcttat tgaatatggg ccttgctaaa agccctgtcc tctgaggaac tggggacagg 118800tagccgggaa aagagaagat ttgggacata gtaattaagt atttgcgtgt tgtcacattg 118860gagggggcat tgacttatcc acagtaactg cagaggacag agctggggtg aatgggaaca 118920gattatggga ggcagatttt ggccccaggt agagaagagc tttctagagt tcaagtggtc 118980tgaccacaga ataggccacc agatgggtta ggggacttct agccactgga atcctcaaac 119040agggctgggt ggccgtctgt ctgtgatctt gaaaagtcca gttctaagga tgaagtcgtg 119100gtaaatgtcc atggttaaaa ctcgtgacaa aaaagtagga tatcttgtgg gttactgggg 119160tagccatggg gaggctcaca cctatcccct cgtctagctt tctagaagta gaaaaatatg 119220taggagtcag aaacataatg gaactatgaa aagtacatac agcatagatt ttgttctatg 119280acagtcatag gtgtatacat atgtgtattt aacatattca catacatata ttcacatgta 119340ttttgtacac tcacatacat tcgcatatat tttatgcaca aagaaaagtg agcactttgg 119400tatataactg acaaagatgc caacacccag ctctctccac ctggctagat tttggttcac 119460ttgtctgtac tcacttgctt gtttatatgt actcagagca gttctgcctg cacttattct 119520tctgctagcc agtattttac ctgtggttaa ttgtaatttc tctgtgtaat tatttgaaaa 119580tttaaaacaa aaatccatca ctttactctc tgacaatttc tttttttttt tttttttgag 119640atggagtctc gctctgtcac ccagactgga gtgcagtggc ccattctcag cccactacaa 119700gctctgcctc ccgggttcac atcattctcc tgcctcagcc tcccaagtag ctgggactac 119760aggcacccac caccacgcct ggctaatttt tttttttttt gtatttttag tagagatggg 119820gtttcatcat gttagccagg atggtctcga tctcctgacc tcgtgatctg cctgccttgg 119880cctcccagag tgctgggatt acaggtgtga gccactgtac ccagccctcc ctgacaattt 119940cttagtagct ttgccttgtg agcattctct gcccttttct tttctctgtg tatgtaacag 120000attagaaccc tcagctatta tagttcagtt acagcagaag ttctcttcat ctgatcatgc 120060ttctctggct tcctagagtc actgatgatc ttcatttcct ctgtagaaca tcctgccagt 120120gcccatagcc tcacagcgtg tattattggt tattctctca aacacctaaa catttccatt 120180cccaccgctt cacattatcc ttgtcagaaa ccggtgggct tcttttcaaa cctgtttcta 120240ctcactgtaa ttgttacatt ataaaattta attaaaattt actcaaacat attatgaata 120300ggaaaagaca agtttggttt ttttctgtga aagtttagtt aaggccgggt gcggtggctc 120360acgcctgtaa tcccagcact ttgggaggcc aaggcatgca aatcatgagg tcaagagatc 120420aagaccatcc tggtcaacag ggtgaaacct tgtctccact aaaaatacaa aaattagccg 120480ggcgtggtgg catgtgcctg tagtcccagc tacttgggag gctgaggaag gagaatagtt 120540tgaacctggg aggtggagct tgcagtgagc cgagatcgcg ccactacact ccagcctggt 120600gacagagtga gactctgtct gggggcgggg ggaggaggaa gtttagttga aagttttgaa 120660taaaatctta aaggactaat agctattgag ataggtatgg gtgagactgg gggaaaaaaa 120720cccataaacc ttgggagatc ctgaattcag aattctttag aagtatctag gttcttgctc 120780tgtttttgtt ttaaagaggc tgaaactgaa aatccagaga taatatctta tgtgtatgtt 120840tatgcagaaa agtgactttg tctaattggc ccagatgctt aaagagaaag ccttggcact 120900ctgacaaaag attgcaaata aatgttttaa gttttaagtt aaactatttt aaagtgagta 120960tgtgtgtgtg tttaaaaaat gatttccaag ttagtcttaa gaatgctttt attatactag 121020gatccgttgc acagctattg ccctcatggc tcaaggcagt gtatgcaggg aagagcatgg 121080aggttggatc ccatagagtc tatgtttcat tttcgtttca tcacttcctg ctgactgtaa 121140ctgtgctcaa actactgaat cacctctttg gcccttggtt ttcatgtctc tgaacagaga 121200tatctgcttc acttggtttt gtgaacaata agtatgaaaa catatatgaa gacttagcac 121260aatatctgac actcaatttt agttttcctt ccattctctc tccttccctg aaaaactcat 121320atgagctttg atacaacact gtttcatgag acagagtaca gagggatagt taaagaagct 121380ttcatagaaa agggaatgag agaaaggttg ttgtatttag ccagaaagtc taagaaaaga 121440ctgtattctc tttggagatt atggaagaaa tgagatgggt tgttgcacat atacaatggg 121500atattttgcc cttcactgac catagagaaa gatcattaga gatgaacttt cttaactctg 121560tctctctcct ttcccatcac tcttcttatc tgccttcccc aaaaccctct atgcatgctt 121620tttcttctat caggtttgga ggactagaga ttctacctgc ttgttggatc ctcctgcacc 121680atcctgcttc ttttattttg aaaccatgta gtctgttatc acccttttct tctgaatttc 121740tgatcttgtc ttttctactg aagtatggat gtggtcatat aatggtagga caacacccac 121800ctagactaac tttatggatg aaacttcatt ataaggatat actgaaatgt aaggagccag 121860gaaatccctc tgaatagcca tgtatttggc ctatatcccc atattgggac aatagctcaa 121920catattttgg gtgccatatc tttatatacc tgctgtatac tcttctgtga aagggatttg 121980ataggtgggt agtataaaat agtggttaaa agcaccagct ctggatttag gctactgctt 122040gggtttagat cctgcttctg ctattttcta gctgtgccat cttagacaag ttatttgagc 122100ttatgtttgg ttcctcttct gtacattgga gacagtaata gttcctgtac tgtagggtag 122160ctgtcaggac atgtgcaata tgcaatgcct ggtgcataga agcttccagt agacattagc 122220tgccatttag tgtcatttat cactacgatc atcatcatct ttggctgggg ctatttacca 122280ctgcctaata tggagcactc gatttggtgg agctgctcat taagctcact cagaggcagc 122340tgcctaggtt acagtgtcaa agccacaaca ctagaaacat ccttgataga aaaatgagct 122400ccttgtcaag ggctttattg agtctagaac ccccagaatt cactacagga cctagaatgt 122460tagactttgt cagtgaaaat ttgtcaagta aatttgaacg tatgaattca aaatctctca 122520ctttgggtat gtaaagggta tataaatctg ttttgtaaat tccttatcct tatatactct 122580atactctaca aaagagaaat gtatgatcag aaaggtgctt tttttttttt cttttttttt 122640ttttgagaca gggtatcact ctgctgcagc ccaggctgga gtgcagtggt gcaatcttgg 122700cttactgcaa cttttacctc ctcgggctca attgattctc ccacctcagc ctcccgagta 122760gctgggacta caggtgtgtg caccaccatg cctggctaat ttttgtattt tttgtagaga 122820caggatttca ctatgttgcc caggctagtc ttcaactcct gggctcaagt gatccccttg 122880cctcagcctc tcaaagtgct gggggattac aggcatgagc caccttgcct agcagaaagg 122940tgctttttaa aactatacat tttgcagcaa accgccatgg catgtgcata cctatgtaac 123000aaacctgcat gttctgcaca tgtatcccag aacctaaagt atattaaaaa aattaagaaa 123060aacatacatt ttgctccatt ttatcctggg tgtataattg accttagcat tctgcttgat 123120tactaataaa atgaattgta ttttaggcct ttaattcttt tagcagtaaa tttggttcaa 123180attttctgaa taaatagagc cctttcttct actataacta gtcaatgtta agaggaaatt 123240ctgacaaatt ttcctgggag ccaataattt aaatttgctc acattttcta actaatattt 123300atttttaaaa atgtaaacaa ttgatttagt gaataaacat aatgatgggt gtataaaacc 123360aagcattttg cagatttcaa cttttagggt ttcttttttt aagggaaatt catataaaag 123420ttataaccat gctaatgaca tccttactta caacatgtct ttcttaattt ccattttaca 123480ttttttgtct ttaaccgatg aaaacttata aagatgttgg tgctcaaatg tatagggatt 123540tggaagttat atttttgttg ttgatttcca tttttcttat cgtcagagaa tatgatctga 123600ataataccta ttttaagatt ttcttcattg cctagcatgt gataattttt gcaaaatatc 123660tatggccttt gtagatcaag cttgttaatt atgtggttca aatattctgc attctgactt 123720tttgctcttt cagctgttga gagagagatt taaatagccc gttataatac tgcatctgtc 123780agtttctctt tttctttcag ttactttttg tattgtgttt ggaggctgtg ttttattgtt 123840tgtctattta tttatttatt tatttatttt ttcaacccaa gtcttgctct gtcacccagg 123900ctggagtgca tggcacggtc tcggttcact gtgcctcctg ggtttgtgcg actctcctgc 123960ctcagcctct tgagtagctg ggactacagg aatgcaccac catgcctggg taatttttgt 124020atttgtagta gagatggggt ttttccattt tggccaggct ggtctcaaac tcctgacctc 124080aggtgatccg cccaccttgg cctcccaaat tgctgggatt acaggcatga gccagcgcac 124140ctggcctttg ttgttttgaa gtatatgagt ttagaattat ttatcttttt aaaatattct 124200agcgatgagt ctccttatct ataataataa tttttgcctt aaagtttatt tgtctggtat 124260caatagagta atgtcaattt atttggttaa ttttgcctgt taaatatttt tctatctgtc 124320tactttgttt ttctatatgt taggtatatc tcttacacct aatctatgtc tagatttaaa 124380aatatgtata atttcagagt cactcttaaa tggtcaattt ggtatttttt gtttattgtg 124440ataactgata tttgggttca tttctatcat cttatttttt gatttaaaaa attttattat 124500gtattttcta cttcttccct tttaggagtt gatcacattt ttatgttttc tttttcttct 124560tttgctagtt taaaagtcat acattctgtt tcaattccct tttatctttt gagacagagt 124620ctcgctgtgt cacccaggct ggagtgcagt ggtgtgatct tggctcactg cagcctctgc 124680ctcccaggtt caagtgattc tcctgcctca ccttcctaaa cagctaggat tacaggcatt 124740tgccaccatg cccagctaat ttttgtatta ttagtagaga tggggtttca ccatgctgcc 124800caggttggtc ttgaactcct ggcctcaagt gatccgtccc ctcccgcccc acccgccgaa 124860accacctttg gcctcctaaa gttctgggat tacaagtgtg agccaccatg tttggccatg 124920tttcaattcc tttaatgacg tttatgtttt gtaacgtgtt cttgattaat ttgagaattt 124980aagcttctat cttcccaaaa aagaatctta gaaattctaa ccaaaatcat tcctctctga 125040ttttgcatgt tattgtttgt tattttggtt ccaccttgtt tctatatcac taaaacttaa 125100ttcttgggcc ggatgtggtg gcttatgcct gtaatcccag cactttcaga ggccaaggca 125160ggaggatcac ttgagcccag gaattcgaga ccagcctggg caacatggtg agaccctgtc 125220tttacaaaaa atacaaaaat tagtcagatg tggtggtgca cacttgtagt cccagctatc 125280caggaggctg aggtgggagg atctcttgag cctgggaggt tgaggctgca gtgagctgtg 125340atcatgactg taccaccata cttcagcctg ggtgacagag accttgtctc ttaaaaaaaa 125400aaaaaaaaaa gtaaatccaa agaacaagca tataaatcaa ctttgctagt aattaaaaca 125460tacaaagtaa agcgagatgg tttagttaga ttagcaaaca ttaaaaatga tttttaatgc 125520ccaatgggtt ctgagaaaac agtcaaacta ttgaggactg ggtaacatag taagacccta 125580gttctacaaa aaaatttaaa agttagctgg gcatggtggc atattcctgt agtcccagct 125640actcaggagg ctgaggcagg aggattgctt gagtccagga gatgaaggct gcagtgagct 125700atgattgcat cattacactc cagcttgggc aacagagcag gactctgtct caaaaataca 125760attaaaatag tgtagatact acaatctaat tttgtgtata aaggctgggt gcagtggctc 125820acgcctgtaa tcccagcact ttgggaggcc aagatgggca gatcacttga gaatcaggaa 125880tttgagagca gcctggccaa catggtgaaa tcacatctct actaaaaata taaaaattag 125940ccaggcatgg tggcgggctc ctgtaatccc agctacttgg gaggctaagg caggagaatc 126000gcttgaaccc gggaggctga agttgcagtg agccaagaat gtgccactga actgcagcct 126060gggtgacaga gtgagactcc gtctcaaaaa taaataaata attttgtgta taaaacgtgg 126120aaaaatatgg tggcaggcac ccatagtcct agctactcgg gaggctgagg caggagaatg 126180gcgtgaaccc ggtaggcgga gcttgcagtg agccgagatc acaccactgc actccagtct 126240aggccacaga gcaaggctcc gtctcaaaaa aaaaaaaaaa aagaaaaaag aaaaataaaa 126300gcattaaaaa gactgaaaga gtttatgcca aaatttattc tcttctatat ttttcagatt 126360ttttcactta atttgttatt tgaaatatac ttgttttgtg taagtataag gaaatatata 126420catatgcaca catgcatata aacattttaa gaatgtgtta taataaaagt atattatttg 126480atacctttgg aaatatcccc atttttctac ctgaagaaaa ttcctaattt catggtttgg 126540aaacaggttt atgagcactc tttatagaga aacggtgtta gtatctatag atgacctgga 126600aatggagacc taaaaagttt ctgaaaagtt atgtcgttgg ttttgctagt acggtcacga 126660ccatagtaat ctttggtacg tgccccacag gctccagaaa ataaaagtca agctgctttt 126720gcttgactgc ggttttaccc tggcaattcg aatgactctg ctttcctctt caggctcccg 126780gagagagaga tgtgggtacc gccttgtgcg gagacagaga agtgccgacg agcagctgca 126840ctgtgccggc aaggccaaga gaagtggcgg ccacgcgccc cgagtgcggg agctgctgct 126900ggacgccctg agccccgagc agctagtgct caccctcctg gaggctgagc cgccccatgt 126960gctgatcagc cgccccagtg cgcccttcac cgaggcctcc atgatgatgt ccctgaccaa 127020gttggccgac aaggagttgg tacacatgat cagctgggcc aagaagattc ccggtagggc 127080tttctggcta tcagttttcc atgtacttgt agaaaggccg gccgctaata tttaaggggc 127140aagagtacaa agtagaggtc catgagctgt gcctagatat ttaacaggtc ctcagctgga 127200tttgtaactt ttaagtgcaa tatgttcctt ccttctgtct tggcatacct accttcaaca 127260aggccgtgtt ctgatttaga attctgagac tcttctgagt tctgtaccca acatggtagt 127320gcagaaagag ttgtgcgtgg cccagccatt tctattcttg actgccttct tttcccatgg 127380ctagatgcat cccataccac cttgcacaaa ccctatcctg tgtgtccaca tctgctacag 127440acactcacct gttggccacc tctcatgcct agaggtggtc tgggaggatg gacccaggga 127500acctacctag gctctggaat tgggcttggg gtcatttggg caagaatcct agagtcctgg 127560aacctggaac gtggttaaaa tgatagactc cacattgacc catttcttgg ctgtggattc 127620ctcaccttga aaggaggggt ggggtagagt acagtatgac tagtttgaaa gtgaaaggtt 127680tgtcagatgc taaatagaat tttgtaaatt attgttccag tagagaatca atattatgta 127740cataaatgaa tatgtatgga caaacagagt aaatcagtgg ttgaagttac acgaatcatc 127800aatgggccca taaacctgga atgccatcaa gttaaaaatg agcttagtta ctcatgagtt 127860gtcacttgga acctgcgttt tccatcctcc aaagtgatca cttctctcaa gcccatttgt 127920aatatatatc tgaagtgctg tatgatgcta aaattaccag ctaattatca tttgacttgg 127980tgtttctgtg gaggagtgaa tctaggattc taacctagag tggcaacacc ccacgatccc 128040cctgtgacag cttctccatg ctgttcttta cagtccttga agaaatgaag tctcttataa 128100gttctgagcc actgggggca ttcccatggc ctggagggca gcgactgcac tgggcaagct 128160gtaaagatga ggaggggtga gaagctgggg gaagagaagt tttgggtaaa gagcctgggg 128220aactgaggcc tatggtgaca gtatcatttg gggacttttg ttggcctggg cccatttctt 128280ctgagcttcc tgaggatttt tggtttctag ttgtattttg ttttgtctag catcttcacc 128340tttgccagaa ttattttatt ttctctgctt tttcccaggg gaggcaatac tgatgcactt 128400tcctctagtt tttgctttaa atgtattcca aacacgattt tgcaggacca cacatggaga 128460gcagtggtga aattaattat tgctgaaagc tgtgcacctt ctttgtgcca taagaaatct 128520gaactcttaa actgcattat tccttattca agcctggtgt tttgaaaagt tttcaggaaa 128580cgtagacata atctgaaggc gtgatttttt ttctcctctc ttagctggca tagtcattgt 128640ccaaaccaaa aaatatatat taaaatatca tctagccttg atcttgttga atatctacaa 128700gattaagaac cgtgatctct cttgggtagg cttattgtca atcactatgg gtgagactgg 128760gaaggtatat acacattagg aacctaaact gagcaaagca tgtggattta gaaagtattt 128820atccatcttt acattcataa caccattaca ttctccttga ggcagatttg cgttataatt 128880gttcaaagac ttgaaccatg tgtgttctct ctgctgtagt ttcctcatct gtaaaacaag 128940aatgataaga gatcctgcct ataagacatt ctcagagata ggcattgtta cccccatttt 129000cctataagaa aaacaaagac ttaatgggag attaagtgaa cagctagaaa gaggctgagc 129060tggggttcga accagagtcc atttcactcc aaggcggtgt cttttgttat catatttata 129120ttacatggcc ctctcttttt atcatggctt gtgaaggaag ccccggtgtt ctctgctttg 129180cttttgaagt gcttccctcc ccagagatta cctgtttgca aacagtactg tgaccaacat 129240gggttattag gttgtcagga cctgcttcgt tattatattt gctctttatt tatttattta 129300tttatttatt tttgagacag ggtctcgctc tgttgcccag gctggagtgc agtggcgtga 129360tctcagctca ctacagcctc gacctcctgg gctcaggcga tcatcccact tcagcctcca 129420gagtatctgg gactacaggc acctgccacc atgaccagat aattttctgt agagatgggg 129480tttctccatg ttggccaggc tggtctcaaa ctcctgggtg caggcaatcc acccaccttg 129540acctcccaaa gtgctgggat tacaggtgtg cttggctata tttgctgttt aggatagaat 129600cacccagaaa cagtgcttct acccagaaga aggatcttaa cactggatag gaaattttaa 129660tcaatcagag aaatccttgc agttgaggcc ttggttttct gtgagggctg gcactgctct 129720ctgcaagcct ccaaccccaa cctccaccta ccccatcccc cacctacccc atcccccacc 129780ccttctgatc ccagtcaagg attgggtcag acaggcaggt cttctgactg gcagccaagc 129840atcaacattc tcagtagtgc agaggaatta tcaggacaca gctaacaaag atcagttctg 129900agccgaggtc gtagtgcttg acaaactcta aatgaagtat atttgtctct agaaggggtc 129960caagactgga aactaagttg cgcagcttaa cttcaaagtt ttcttccttt aatgagcagt 130020taatcacatc tataaaatat caactcccta atggtttgtg ttttcttagt gttttaacac 130080ttgccattct gtctctacac acacagggag ctgaggagga ggggtggggg tgtctcaccg 130140cctcttgctt tccccaggct ttgtggagct cagcctgttc gaccaagtgc ggctcttgga 130200gagctgttgg atggaggtgt taatgatggg gctgatgtgg cgctcaattg accaccccgg 130260caagctcatc tttgctccag atcttgttct ggacaggtga gaaaaaatac attgtgtttc 130320ttctctgact tgtttgagta aggtgcttag tgagtgggaa caaagtcctg ggtgctgcaa 130380ttaaaatctc acacttgcag ggcagaggat gatagcatca tcagctcctt cactgggtca 130440agaaccagag aaggagagag ttgggtccaa ggattcaggg tcctgtgact catttttaat 130500ctgtggtgca gcagcattta caggccagcg ctttaatagg ggactgtatc ccgtaggtat 130560gtggccacta tgtgtataag tcgacacaga tttttctcca ttaaaaattc cattttcagg 130620ttataatctt aagttgtcct gctgtttttt gtacctatag tgaccaatta tatctggagc 130680tttctggaca ggtgataaaa ttcttagaaa tgtgccaagt ttattttcac atgctttaac 130740tcactctttt gttttttttt gttttgtttt gttttgtttt ttgttttttt tctgagatgg 130800agtctctctc tgttgctcag gctggagtgc agaggtgcaa tcttggctca ctgcaacctc 130860cgcctaccgg attcaagtga tcctgctgcc tcagcctctc aagtagttgg gatcacaggt 130920gtccaccacc atgccaggct aatttttcta tttttagtag agaagtggtt tcaccatgtt 130980ggccaggctg gtcttgaact cctgacctca ggcgatctgc ccacctcagc ttcccaaagc 131040gctgggatta caggcgtgag ccaccatgcc cgatctgctt taacacattc taatgcatgt 131100actatatagc atttttggca atagcggtgg aaggaagggt tactaaaact atatgaaact 131160taacagaaaa tgggacatga tgctgtatct tggttgtgtt tgattttctt ttaaagatga 131220cacagaaaag gaaacaattt ttaattgact taggtgaact gtttatggag ggaaagctgg 131280actgtataaa aatactcaag ctttttagca ggaaagtaga acaccctctt ggtgtaaatt 131340cgagcagttc gaaatcttct tggaaattga tttccacatc tcttttatgg aaaaagtgct 131400aggttgaatg ttcagccaca tctgactctg catagcgtgg gaggatgcct agtgtctacc 131460ccaactcttg cattataatc ctgttaccac tttagatcat cagaagaccc tgtgttacac 131520agatgaagag tgatgcccca aggtatcagt ccccattctg ccttttgtca tggttgacaa 131580tgttattaaa agagcactgt tctgcataat ggtgttttga tagagaacag atcctctgag 131640aagagctgga ggactgatgt gacttgaaca ggagcaagcc caggtggtaa accatggagg 131700gaggctctgg aagaccagag aagttcaggg cacaagaccc ttcagtaaca aacaaaatag 131760ttaacctatt ggcttgtatg tgcttggcag caccttatgc atttaactta tgtcaacaca 131820tttaatcttc acaatcttcc tgcccccttt gagggagtag gatccattat tatctctatc 131880attcagatat tggaaatggg agattgagaa acctgcttac aggtaggata ataggtggtg 131940gagctggact tggggggttg ccaaatggca aactaactct ctactttatt ctacctgttg 132000ttatgggtga caatgttgac aaagagcaca ttctgcagaa cagagatgtt ttggtagaga 132060acagccctgt tttacttgta acacactgca gaaacccact ctccccactg tcatctcagg 132120gtaccatgtc gcaaggcagg ctgaaaagcc aagcacctag ccaagccatt gctctcattc 132180attcattgta ttctgcttgg tgttttaact ggggccaaat atacatatgt ataaatatac 132240acatataatt ttccttgaag ttagtcctag gaacacattc catcccttga caaataattt 132300gcagacttta ggattatttt atcttttgtc ttgatttcta aattgatgcc aaatttagtg 132360tttatttttg gtgactattt cattcctggt ttttagtaca attaactctc cactctccca 132420tttctctgta tgcgttcttt aattcctgta attgtgtgta tacattacta taagtggaca 132480caaatcctgg aaaaatatta ggcctacctt ttagttaata gaagaaaagt tatttttctt 132540acaaattatt tctaatagac ttacactgcc tttataactt aagtgaaagt attatgttgt 132600aaaacataaa tctagtatat ttgattgagt atagaagagg aattcttggg aattgtaaat 132660gcattcatgt tgagcaggca tttttttttt tttttggaat gactactggt gtttatttgt 132720tgttgcaatt tctagtagtt tttgtttgtt tgttttttgt ttttgagatg gagtctcgct 132780ctgtcaccca ggctggaata cgatggcatg atctcagctc actgcaatct ccgcctccca 132840agctcaagtg attcttgtgc ctcagcctcc tgagtaggtg ggattacagg catgtgccac 132900tacggctggc caatttttgt attttttttt tttttttagt ggagacgggg ttttaccatg 132960ttggccaagc tggtctcgag gtcctgactt caagtgatcc cccagcctca gcctcccaaa 133020ttgttgggat tacagacgtg agtcaccacg cccagcctac agtctctagt atttttaaca 133080cattaacttt ctgaagtctg gaacttgaag tctaagatag ttcagttact tagtcctctc 133140ttatacaaat gaatatactt ttatgtaata ggtatatttg tagaggagtt gctcattcaa 133200aaagtcagga gtcatgctcc ataaagactt ctattacgac tcttttttgc aaagtgaagg 133260gaatcttcac accatttgaa aataactgtc ttctgctgga ttgtcctagc agagcttctt 133320caagtggtaa tatggctgaa taaacagtga atacaactaa cagttgccca tttgtggata 133380ctgaaactat aatttctgtt tccctttatt cttgttgagg tgtccacaac aagaaaactt 133440gtgtctactg aggatgagag gaaaatctca ttacttcagc ttatttctaa gcatttagtt 133500tttcttttac taaccactaa attcatcata aattcacgtg aagatctaaa gaacctgact 133560gtctaattgc tcaaaaaaaa gtcacatatg caaagacatt tttgtgtcct tagtatcaac 133620aggcaactga ctaatgttaa attattagtc agaggaagtt tgtatctggc ttggatccca 133680ttgtggacat ttgcagatag gtccgtgaaa ttgtatatgt ataaatgtct tgagtttaca 133740ttcacattag ttatttgtat gctaaattcc ttcaagataa ccaccgaatt ttcaattccc 133800aattctaagc cttaaacact ccctgccatt gccatacaca cagaggtaaa ccatggtctg 133860tacccaggtg tgtgctgcga gcagagatat atatatatat atacacacac atacatacac 133920acacacacac acacacacac acacacacac acacacacac acacacacac aaatagtgta 133980cccctaaggg aggcccactc attcaacatt ttgttgttgt attaaacaat attcttcttt 134040aggccaggca cggtggctca cgcctgtaat cccagcactt ggggagactg agatgggtgg 134100atcacctgag gtcaggagtt cgagacaagc ctgagcaaca tgatgaaacc ccttctttac 134160taaaaataca aaaattagct gggtgtggtg gcaggcgcct gtaatcccag ctacttggga 134220agctgaggca ggagaattgc ttgaacccag gaagtggagg ttgcagtgag ccaagatcac 134280gtcattgcac tccagctggg gcgacagagc aagactccat cttaaaaaaa ataaaaaata 134340aaaagcaata ttcttatttt ataaagagtg attattggcc gggctcggtt gctcacacct 134400gtagtcccag cactttggga ggctgaggtg agtggatcac ttgaggtcag gagttcaaga 134460ccagcctggc caacatggtg aaaccccttc tctactaaaa atgcaaaaat tagccaggca 134520tagtggtgtg tgcctgtaat cccagctaca tgggaggctg aggcaggaga atcacttgaa 134580cctaggagga ggaggttgca gagagcagag atcatgccac tgcactccag tctgggcatc 134640ggggtgagac cctgtctcaa aaaaaaaaag tgattgtcaa gtaataaatt tgatatggtt 134700tggctctgtg tccccagcaa atctcatctg aaattgtaat ctccacgtgt caagggaggg 134760atctggtggg agtgattgga tcatggggat ggtttccccc atgctgttct catgagagtg 134820agtgagttct cacaggagct tatgctttaa aagtgtttgg cagctcccgg ctgtcttgct 134880cagtcactcg ctctcctgcc tccatgtaag atgtgccttg gtttcccttt gctctctgcc 134940atgattgtaa gtttcctgag gcctccccag ccatgcagaa ctgtgagtca gttaaacctt 135000ttttcttcat agattaccca gtctcagata gtgctttata gcaatgtgaa aatggactaa 135060tacaaagata ttccatgtta ttactgattt tatttagtag tttatggaca gatagtgtgc 135120aaaaataaat ttcctgagta ggtcagtttg gttgatacat tgtttcaata ttttaacatt 135180caaatcatat gccctgtttt tgtttttgtt tttttttttt tagagacggt cccgctctgt 135240ggcccaggct ggagtgcagt ggtgccatca cggctcactg caaccttggg ctcctggcct 135300caagtgatct tttgcttcag ccccctgagg aactgggact ataggtgtat gctaccatgc 135360ctggtttatt attattttgt agagacaagg tcttgctaca tcgcccaggc tggtctagaa 135420ctcttggcct caagtgatcc tcccactttg gcctccccaa agcacgagga ttacagacat 135480gggccacttt acccagccag ccctgtcttt aattcaactc ttttaaccct gtcctaattt 135540cttactcata attcagtttc aatctaaaat tataaaataa ataaaataga tgtcattaat 135600ttagagtctt tactaacttt gttctgtgta actcatctga aagaccttta ctgggctgtc 135660atttacgatg tcttatactt tattgcctct tcatttctca tttattttgt tgaaatgtat 135720ttggtttctg cagactgtgg agtgaataaa aatattctaa ctttgccacc ccttgaatga 135780aatggttgac tgtcacacct gcttaaaaag aaagcaatca gacctagttc tttagacttt 135840gtttagaaat taactttctc catgagttat gtatggtctg attactgtga gggacagcct 135900ttatcagggt ttaaaatacc tcagtatgcc aaccctcctc ccatttttgg aacataaatt 135960tgcagtgaaa atggcatata ttttaatgag gaaatgatac caatttcaat attgaggaac 136020taaggcacag gtacttctgg aaaataggat tgatttcagg tgggttccct taccatacca 136080cttggggagg ggtgtgtgtg agtgtgtgtg tgtgtgtgta tgtattgtgc atgtgtgtat 136140aatcccacat cagcacagaa gaataaagag ataatcaaat atcaatgcag gagttggtgg 136200gtttttttgt tttttttttt ttttgcccca tagagatatt ttcaaactag ctttccttag 136260tatcaaatgt ccccaagtcc aacagttaca atttccaata attaattgtc cgcaggcaag 136320gtgattcagg tgttttttgt gttatctctg tgcagggctt gtgttgtcct tactggatgc 136380ctgcatcagg ttgcctggga gagcctagag ctggggaggt ggaaagatga ggcttcctgt 136440agatttggca ctctttgccc agtgctctgg attctctaag acggcctttt cctatgagtg 136500acttccaggg ggcactggtg ttttgtcact taacctgtgt acttatagaa aattgcaggt 136560gtttacagaa tttatgattt agtaaattta gtaacttagt aatgctcata taccaaagtg 136620agcaatttgc atgcttgtag ctctgtgtga gcgagtctgg gtgggagagt gtgagtgctt 136680cggaatgcag gatcccggtg agtgccatgt acggcaggta atgggaaaga cttctgcagg 136740actggtgtat ccagtggtgt cagaggctct tccctgaaat actgccatcg ctggaaatgc 136800cctgagttcg gggaaggagg aggggagcag ccagctcttt gaagacctca aggccccttc 136860aggggctgct agagactaaa aatggaactc gcataaaccc actgcccttt ctgtgtgctg 136920caggcttttg ggagcaaagg gtggttttgt gacaaaatca tctaactgct tgtcaaggac 136980ttccaataac cctgtgactg acaataatag agtgttttgg gggagcagtg aggtggaata 137040atgtgtgtct ggctggagtg aatagaagct ggtattttcc agataaagtt caagtaattt 137100acttccaaag tatatttaaa catttatttc tacaaggagt gctccaaaga attttgatta 137160gatggctcaa agtttaaaga taatccttgc ttgaagataa tccttggcaa gtcaaaaatt 137220tttccccacc tccatgtata ccttcttttc ctgattctaa tccatcttct ctaattgcga 137280tttctttctc atagtcagct ttttcaaatt acaggtaaat gtcttagttg ctacacaagt 137340ttctaagtga ccaccaggaa gtgagagtta agccctagat atggagtttt attcttggga 137400tatttgcttc tgtgacacac ggtcttcctc attaatactt cccgatggga acatgaagtg 137460tctcattttg aaatacgtgt catatctggg gctggttgac tgatatggtt ttgattgaaa 137520acaatcaata ggagtggtat tgctgtaaga aaacatttgg tgaaaatgta gaaggaaaat 137580attccaatgc acatttttgc ctaaataata tttattcata tatttacttc agggatttat 137640aggaaatggc ctatcttctt tatatgaaga caattctagt aatttcatat tgctgggtgt 137700ggtctcatta acaccctgtt gtagttaaaa tgatattatc agatgaacat gttacaagat 137760gaaacttgag attaaaaata aaacattcct tattgttttt ttgatggttt cctgaagcta 137820tgttccttaa atttccaaac gaacttttgt agggatgagg ggaaatgcgt agaaggaatt 137880ctggaaatct ttgacatgct cctggcaact acttcaaggt ttcgagagtt aaaactccaa 137940cacaaagaat atctctgtgt caaggccatg atcctgctca attccagtaa gtaatcacac 138000agctgggcca tgttttatcg gggagagatg ctgtttctac aactagcgtg atattaagaa 138060gaatgttgaa cttctatttt atttgaaagg gtaaaatggt ttccttttgg acttcgtttt 138120tattttgata gcgatttaaa ctgtaggtaa cttttggtaa cttggacata aattactcat 138180taagtgaatg actggcaatc aatttaaaag tagctcaagc cacttgctgg aaaagaaaaa 138240aaaaggaact ttaaattgtt tatcttttaa acttttttca gtgctcacac agacacttta 138300catggttggc atgcatttat acttatgtct ggggtcctcc ttttttacag attcattcgt 138360tcagtaaaga tacaatccta ccctcaaatg gctcatagtt taggcaggga gagagagaaa 138420acaaatcatt aaaaataatg atttctgtgc tatgataaag tctacacaaa atactacggg 138480aaaataggag gagagatgct ggagttgttg cagaagggaa tgattgaaca aatcttcagg 138540aaagagcaga gggaagtagg tatgacttta aaatgcagtg ctgaagatta gaaactgctg 138600cccaggcttt gggcagctta gaagaggttc aggcagggga gtgtcatgca cagatatgcg 138660atatagaaag gtcactctgg cttccatgtg gaggactaga aagggcagag actgaagccg 138720ggggcccatt agaggcaatg agagcctgaa ctgacattat ggcgtgaggt cagggagcaa 138780aggacttgac ttgaaggaaa agtgggaggt agaggaggga aataaggtgt ctaggatatg 138840cagatggttt cgttttgttg gttttatctt atataaatat ctgattattg ttaataaaca 138900ttcaaatgag aaaaacatac aaggaagaaa ataaaatcat caggaatacc tgccctcaaa 138960ataaccacca taactttggt gaccattcct ttctttcttt ttttttttct tttttttttt 139020ttttttgaga caggatcttg ctctgctgcc caggctggag ggcagtggca tgatcatagc 139080tcactgtaac ctagtactga gctcaagtga tcctcccacc ttggcctcac aagtagcttg 139140gaatacaggt gcataccacc agacctggtt aattaaaaca attttttttt gtagagacag 139200aatcttgctg tgttgccaga ggtggccttg aactcctggc ctcaagcagt cctcccacct 139260cagcctccca aagttctgga atttacaagc gtgagccact gtgcacagtc tgtatcttgt 139320tttttcactt ttctttttga gacagggtgt cactctgttg cccaggctgg aatgcagtgg 139380cacgatcatg gtttactgca gttccgacct cctgggctca agtgattctc ccacctcagc 139440cacctgagta gctgagacca caagcacctg ccaccacacc cgaataattt ttgtattttt 139500tgtagaggtg aggtatccct gtgttggcca agctggtctc aaactcctgt atttttgttt 139560ttcttttcaa aatgctgtga aaccattctg ggtcaactag gagagatcta acacaatctt 139620tgatatgagg gcattatact aaattgttca accatttctc tgttattaaa tatccagttc 139680ctcttccttt ttaaccatta taaacattac tgcaataaat agagatgtgt tattttgtat 139740gaatttctaa gtttctggat ggttgtcaag actggtcatt tcacgatcta cctggtgtct 139800aagccagccg ctctagcaga tattgatggc tttgcttagc catttactct tgtcgagcct 139860ttaggttatt gacttttttt cttcctcaaa cactgtatat ccaggtttta atgttcacct 139920gaagacttac agatatctct atttagacaa catattgggc cttatttatc caatcttaga 139980gttcgatact tgaaacaaca gggatatatc agatcatatt atactacggt ctttaaatca 140040gccaaagtag cagttcctga agccaagatt caatgcagaa ttcactgtgg tcacatgttt 140100ccagctgcct cttgatctgg ggccagctga ccttcatacg tgtcttctct cacagactct 140160tggatattgg cactagtttt tttgtttgtt tgtttgtttt gagacagagc ctcactctgt 140220tgaccaggct ggagtgttaa ggatagttac ttttgcaaaa tataacaaaa atgaattatg 140280agaaaaataa aagtgtaaaa tacaagtcct agtttataat attattagtt atcacattca 140340actgatttaa aattactctg tcgattgcta aaaatgttcc taaatgctta cactcaattt 140400ctacccatct ctttgtaaat gggcaacaga ccatacatta gctctggaga gagcacagga 140460tactgtccaa gagttgcttg gatctagggt ggagggtggg gttagcccct gaagaactgg 140520gtgagggaga atgaagaagg aatctgaagg ccatgtgaag gtacagagac ctggaagaca 140580acctttgagc atttcctagt taagtctcaa tctggcctta cctgcctaac aggtcatttc 140640ccctgcaccc aacacaccct tccctgttta ttgttaccat tcacctttta cagaataaca 140700tgagggccca gcttatactg atgattatgt tgatgtgcat ataaggaaag tccagccagg 140760tgtgtttttt tttttttttt tttttttttt ttttgacaga gtcttcctgt gttggccagg 140820ctagagtgga gtgcagtggt ggaatcttgg ctcactgaaa cctccgcctc ccaggttcaa 140880gtgattctca agcgatgcct cagcatccca agtagctggg attacaggtg catgccacca 140940tgcctggcta atttttgtat ttttagtaga gatgatatat tagtctgttc tcacactatt 141000atgaagaaat acctgagact gggtaattta taaaggaaag agatttaatt gactcacagt 141060tcagtatggc tggggaggcc tcaggagact tacaataatg gcggaaagtg aagaggaagc 141120aagatacctt cttcacaagg tgccaggaag gagaagtccc aagcaaaggc agaagagccc 141180cttatataac catcacatct tgggagagct cactcactgt catgagaaca gcatggggga 141240aactgccccc atgattcaat tacctccacc tggtctctct cttgacacat ggggattatg 141300gagattacaa ttcaagatga gatttgggtg gggacacaaa gcctaaccat gtcagacagg 141360gtttcaccat gttgtccagg ctggtcttga actcctggct tcgagtgatc tgcccacctt 141420ggcctcccaa agtgccgtga ttacaggtgt gagccaccac atccggccca ccagccagga 141480tatttgaaat tgatcatgga ataagatcaa ccctttctga ccttttccaa accacctacc 141540aacattacct cacataggtg ctgccatttc tgtcaaaggg aggatctgct tgaagagtac 141600cttcccatct tggcaatgga agatcatcaa atgccagatg atggggcttc tctcactttc 141660agaaataatt tagatctctt ttctgtgcag gaaagtgctt ctcggaaagc actgtttgct 141720tgttgttaca acactttaca gtataaagcc ttctgtttgg caaggctcct tataggcatt 141780ttagcctccc agacatcata ttgtgttgtt gtcaaagcta gacgcagcat ctgtgcaaat 141840gggaaagatg aaggctacag cattcccctg cagcatgaca gaactgccac attgagataa 141900ttacagaagg cgagggagac atgtgatgta attaccactt gtggcagtaa acgagtaaaa 141960tttgtttact gtaaatccaa gtttaagaaa tctttttttt tttttttttt ttttttttga 142020cagagtcttg ccctgtcgcc caggctggag tgcaatggtg cgatcttggc tcactgcaac 142080ctctacctcc tggcttaaaa cagttctcct gcctcagcct cctgagtagc tgggattaca 142140ggcacccacc accacgcctg gctaattttt gtatttttag tagagatggg gtttcaccat 142200gttggccagg ctggtctcga actcctgacc tcgtgatccg cttgcctcgg cctcccaaag 142260tgctgggccc tcgcctggcc agagatcttc ttttaaggaa gttcctttct tggtagtcat 142320aacaattgtc aaaataaatt gatcctgttc agcattgcta tggcgaaaat gggacaattt 142380tcactgctag gttaagtgag tcttttctat gctaggtttt aaggatttgt aagtacaggc 142440ttttttcttc tggattattt gtggtattta aatttaaaaa aaaataggga tggaatctgc 142500ctccccgcct taaaatttaa aaccctgaca gaatatataa aacagatatt ggacattgga 142560caacagtgat ccccaggagg agggacacaa acgaggagag ccctttgatt gtccagttta 142620ctgcctggag ccagtttcca ggttgcaaag cagggatggg tgtgttaggt ttctccagag 142680aaacagaacc aataggatgg ataggtaggt aggcagataa atgagagggg atttattatg 142740aaaactgact tgaacaatta tgaaggctga gaagtcacat gatatgcgtc tgcatgctag 142800tgaaccaggg aagccagtag catggctcag tgtaaatgga aagacctgag aactagggag 142860ctggtggtgt aaccctcagt ttgagattga aggcctgaga aactgggagg ccactggtgt 142920gagtcccagg gtctggaggc tggagaacct ggagttctga tgtccaaggg caggagaaaa 142980tgggtgttcc agctccgaga gagagaattc tcttcctctg ccatttcgtt ctatctgggc 143040actcagccaa ttggacggtg cctgccaaca ttggctaagg gcagatcttc cttacttagt 143100ccactgttct ttcttttttt ttttttttga gatgaagtct tgctttgtta ctcaggctgg 143160agtgcagtgg tgccatcttg gctcactgca acctccacct tctgggttca agcgattctc 143220ctgcctcagc ttccagagta gctgagatta caggcatctg ccaccacgcc tggctaattt 143280tttgtatttt tagtagagat ggggtttcac cacattagac aggctggtct cgaactcctg 143340acctcaagtg atccacccac tttggcctcc gaaagtgcag ggattacagg tgtgagccac 143400tgtgcctggc cttagtccac tgactctaat gccagtctct tcctggaaca ctctcacaga 143460catacccaga aataatgctt tatctgctat ctgggtatcc ctttatccag tcaagttgac 143520acctaagact aaccatcaca aagggtaacc caaatagaca ccagtggtct cccttggtag 143580caaggcagct aggacttgga ggggagagta ctgagtggga aagagctgca caaagaattt 143640tggagatcta tggagagtcc tcttcaagtc ttcagctgag tgctaatctg cccatgctta 143700tgaggatacc aaggacaggg aaagaaccat cagaaaggag cgggcgaaac aatccctaga 143760gttcacacag ggccaggaac agttcacatt ctcaccagcc agtgggaaaa accttgcagt 143820tcactgggta ttgggcactt ctcagccttc ctatagtatt cagaagggta ttgcctcagt 143880agtgggccta gactaaaagc cattatgatc ctaccaaaca aaaaagcaag cctggaggat 143940caaacaattg ctaagtgatt taactgcatc ccagcacaaa gctcaagagt agagacacat 144000cccatttcat tacatgttgc tttattgtgc atcacagata ctgcatgttt ttacaaatcg 144060aaggtttgtg gcaatgctgc attgaacaag tctgttagta ccattttttc caacagcatg 144120tgctcacttt atgtctgtgt caaattttga taacactttg caatatttct aactttttca 144180ttatatctat tacagtgatc tgtaatcagt gatttttgat gttactattg taattgtttt 144240ggggtgccac aaactatgcc catataagct ggcaaactta acctataaat ttgtgtgttc 144300tgactgctcc accaactggt ggccccacca ccatctggaa ttctgggaga attctaccat 144360gcatttgagg aaggaataat accaagtgat atggtttggc tctgtgtccc cacccaaatc 144420tcatcttgta gtgcccataa ttcccacatg ttgtgggagg gacctggtgg gagatgattg 144480aatcatggga gcaggtcttt actgtgctgt tctcatgata gtgaataaat ctcacgagat 144540ttgatggtta tataaaaatg ggagtttccc tgcacaagcc ctcttctctt gtctgccgcc 144600acatgagatg tgcctttcac cttctgccat gattgtgagg cttccccagc catgtggaac 144660tgtaagtcca ataaaccttt cttttgtata ttgcccagtc ttgggtatgt ctatcagcag 144720tgtgaaaatg gactaataca ccaagtttac acatactctt acagaaaatt gaacagcatg 144780gaatgttttc caattcattc tgtgaggcca gcattactct gatagaacac tcagactaac 144840acactagaag aaaagaagac aacagaccaa tttccctcat gcatgtataa gcaaaagttc 144900tctaaatttt tttttttttg gtaactagaa tccaaaactg tattaaaaga atagcacatc 144960atgaacaagc agaatttttg ggaatacaag gtttctttaa catttgaaaa tcaatcaaaa 145020ttcatattaa cagaataata atgaaaaacc atatgatttt atatatatat atattttttt 145080tttgtttgtt tgtttgtttt gttttttttt gttttttttt tttgagacag tctcactctc 145140cgcccaggct ggagtgcatt ggtgctatct cagggctcac cgcaacctct gcctgctggg 145200ttcaatcaat tctgtctcaa cctcctgagt agctgggatt ataggtgcct gccaccatgc 145260ctagctaatt tttgtgtttt tagtagagat gaggtttcac catgttggcc aggatggtct 145320caaactgctg acctcaggtg atccacccgc cttggcctcc caaagtgcta ggattacagg 145380tgtgagccac tgcacctagc catgattatc ttaatagatg cacacagcat ttgacaaaat 145440ccaacatcca ctcctgctaa aaacactgta caaacaagga atagaaggaa acttcctcaa 145500tccattaaag ggcacctatg aaaatcctac atttaatatt atacttaatc acaatcagga 145560acaaggcaag tatgtccact gtccttaatt ctattcaaca ttttactgta agttctaccc 145620agtgcattaa ggcaagaaaa gaggtaaaag gcatcaatat tggaaaggta gaagtgaaag 145680tctttattta aaaacatgag aatctatgta gaaagtccta aggagtctaa aaaatgtgaa 145740tttagcaagt ttgtaaggtg taagggcaat atatataaat caattgtatt tctgtgtggc 145800accagtgagc aattggaaat tgaaatgaaa aaccactacc atttacaata gcatcaaaca 145860ttgtgaaacc ttgggaataa acttgcaaaa gacatgaaac ctgcacacta aacactgcaa 145920aatatagctg aaggaaatta aagaaatcct gaataaatgg agagagatgt taatggatca 145980taagattcag tattgttttc aatctataga ttcaaactga taaaaatccc aggaggcttt 146040ttggtagaaa ttgataagct gattcttaaa atcatgtgaa aatgcaatgg acatagaata 146100gtcaaaacaa ctttgaaaaa gaacaaactg ggaggactta cactacctga tttagaagat 146160aatgtggtat tgatgtcaac agaaacaaat agatcaatgg aacagagagt ccagaaataa 146220tctatacaac tacagatgtt cctcaattta tgatggggtg atttcccaaa aaacccatct 146280taagttgaaa atattgctag tcaaaaatat acttaacaca cctaacctac tgaacatcat 146340agcttagcct agcctatctt tttttttttt tttttttttt tttgagacgg agtctcgctc 146400tgtggcccag gcgggagtgc agtggcgcaa tctcggctca ctgcaagctc cgcctccagg 146460gttcacgcca ttctcctgcc tcagcctccc cagtagctgg gactacaggc gcccaccatc 146520acgcccggct aatttttttt gtatttttag tagagacggg gtttcaccgt gttagccagg 146580atggtctcga tctcctgatc tcgtgatccg cccgcctcgg cctcccaaag tgctgggatt 146640acaagcgtga gccaccgcgc ccggccagcc tagcctatct taaatgtgtt cagaatactt 146700acattaccct gcagttgggc aaaatcatct aatataaagc ctattttata atacagtaat 146760gaacatttca tgtaatttat ggaatactga aagttactgt actgaaaaac gaaaaaacac 146820aatggttgta tgtgtactgg aagtacagtt tctactgaat gcaaaaactt gcagctgagt 146880gtgttcatta tcttggttgt ggtgatggct ccaccatgta tatgtatgtc aaagtacatc 146940aaatcgtaca cgcaaaatat gtgcaggtta ttgcatgtca ggtatacctg gatgaatctg 147000taaacaatgt aatgaaagca aaacaaaaag attaagagag caaagtttgt aggctaaatg 147060gaaaagaaat accaccaagc ggggaaccaa atcacagggt ggaggccctg gaggataagg 147120gtcaggagag gagaaatggg ggtaggtctc ttaagtcaaa aggctgcgaa cttctctatt 147180ccatgttagg atagcagagt ttccaagcgc tgcatttggt tgctgctaga tggccttgcc 147240aggctagata agcattgggc tgtctgacga tggtctcctg catagtttgg tctcctgttt 147300tcctgtgtat gtgacatgct taagttagga ttatgtcact caatcacatc tgcagtggta 147360cagcacgcta gctggccagg tcgcggtttg tcagtagtca tgttttaaaa gctgcccatt 147420tctgggttat gcatatctac taataatggc tataatatgg aatggaaatt aactgtgtca 147480tccagctaaa tttcagctca gtttctggta tgtatattaa tgacttctaa atactaagga 147540tgtcaaaatg atttagatat aatgcttttg gtctagaatg ggatatatac tcaaatagtt 147600aatcaaaggt ctgatccatg gtgggcttaa gtggagaggc acatatttct ctcttgggga 147660ggcaagggaa aggaccacaa cattctaact ctctcagcca atcctcttcc actatgcata 147720tataggttgt gtggtacttg gaattcctgt atcatactta gcctttgata tggctcttga 147780gagtaagaga caacagaaaa atgttgcatt taacaacctg ttacaatgct tgttagagtg 147840tttttataaa ctctaaggtg ttatgcaagt gtcatagtta ataaaatagc ctacccaaca 147900cccaacagac agactggcca tcttgccacc caaatcctcc cttggataga attagagggg 147960gtatggaatt taggaattag agtgtaatta attacattga ttatccatag tctttaaaat 148020attttaaatt agaaacaagt ctatttaaac agttttaaga tttacaaagg atgaaacttt 148080tcattaaatg aaagaaatag aggggttaag ccaggaaatc ctattttaca ttaagaaaat 148140tattaagaga cactggctta aaccctagtt ccctctgagt ttatagggag agttcccatg 148200gagtgggtgg gtggagaaga caaagacata gatggatgct gatgaggaaa gatgcggggg 148260tccttttctg ttgaccaaga acactggggc aaagcacagt tgaacagcag cctgcagcct 148320cacaccatgg caccttttga gtcccatctg ccctcatgtg ctgggggcag gaggtggtga 148380cagagggcgt gggtcatggc cagaggttcc tttcctcaaa gcaaacaagc aaacgccaca 148440tacggctccc caaagccagg acttcttccc tttggtcagt attctgggac ttctattagc 148500acattagatt tttctcattt atttgccttc agtcaaggaa agcttatgtt ttcatccttt 148560gaacaaatca gacgtggcaa atcttgaagg agaggtggct gtcccccacc actgtgctgc 148620tcagaatgtc accaggtggg ctggtgagag gagcacacag ctgttcccag ctgataaagg 148680ggagagaaga ttgtgtcctt gattttattt cactttcttt ggtatgtgtg aggcatggtg 148740ccaagatctt ggtttttttt gttttttttt tttaaactat acttcttccg tttcatcaaa 148800agtaatttaa ttttgtttta cagtgaatcc taactgatgt ttttactttt gggggatgga 148860gagggtgcta tatttttgtg gttttctgtg cctgactggg cagagctttg gatcttgtcc 148920cttgccccat gctgcccagg gcctgccact tagcaagtac tctgtagata tgtatttgat 148980gagcaagggc ctgagcatgg atgtctgagg tgcaggcacg cactgctgac tggagagcca 149040ggcagcagca tgggtattct tcagcacagt tcttttctgg gagggtattt cttttctatg 149100tgatcaatga gaacaggagt ctccaggata attttatgta agtcagtctt tttgtatata 149160cactgccccc ctaccccacc atatgtaaaa tggatttcgc atatgccttt ccacaactgc 149220agtgcctcac ctccccaaac cgctgtggct gatggactct gggccccagg tggagctgtg 149280ctgcccctac agcctgcaga aggcccaggg tctggccttg gcaatgactg tggttcgtga 149340agtgggtaac acaatgacac atacgtgttc tctgagggga aacttcgttg cacacagccc 149400agggaattta tgttattgta actttggttc tgaggcgttc ttttattatt attattacta 149460ttatttttag taacagcttt attgtgatat aattcattta ccatataatt tatccatatt 149520aagtatacag ttcaatgttt ttagtttatt cacggtatgt ggtgcaacca tcaccaccat 149580caattttaga acattttcat cacctgaaaa gaaaccccat gcttcttagc catcatttcc 149640cactccctat cccacccaca gccctaggca accactaatt tgcttttctg actctatgga 149700tttgcctatt ctagacattt tattataaat ggaatcatac aacatgtggt cctttgtgtc 149760tggcttattt tgcttagcct gatgttttca aggttcatct gtatcagtac ctcattcctt 149820ttcgtagctg aatactattc cactgtatgg atagaccaca ttttgttgag ccattcgtca 149880gttagtggac attccacttt taggctgagt tatgctgcta tgaacatttg tttataatct 149940gaggatttgt ttttatattt tcaatctttg tcactttgaa ctgagacatg tacaggcaca 150000caattttggc tccttttgga attcccagac atagtattgc ttgatggcag cggaagtcca 150060tggagcacat gtcatgcagc tgaacacact acggggtagt taaaaggaag tacttgttta 150120tgcagatggg gttaatttta gggaaagtaa gcttgaaata attttctctg tacttttgat 150180aattttctgt gtgtacctaa aacatacatt agcatgcata tttaccattt caaatatgat 150240gtgtgtttgg ctaaaaaaaa taagggtctg gccgggcaca gtggctcacg cttgtaatcc 150300cagcactttg ggaggctgag gcaggcggat tgcgaggtca ggagtttgag accagcctgg 150360ccagcatggt gaaacactgt ctctactgaa aatacaaaaa atttgctggg catggtggcg 150420catgcctgta atcccagcta ctaaggaggc tgaggcagga gaattgcttg aaaccgggag 150480gcggaggttg cagtgagctg agattgtacc actgcactcc aggctgggtg acagagtgag 150540actctgtctc aagaaaagaa aaaaaaaaaa aggtctgtgc cctcaaagca ctcatgtcca 150600gtcttgctga gggcagaagg gtggctgtgg ggtgtgtgtg gggacaaggc agacatccag 150660catgtggggc aacatggtgt ctctgctgag gatgaacagg gcactgtcag agtatcaggg 150720gacacctcag accagactta gggtgggatg gtagggaggt tgggggaagc ttccagaagg 150780aatttctgac caggttggaa tctacaggag gaatgggtat aaatgagcaa aagaatcagg 150840gtagagaaag aggaaggaga gagtttccaa gcagcaagtt gagcatgttc ggagcaccac 150900acattcaggg agttgagagg gggactcaag gcgaggtgtg gtgggaactg cagatgagag 150960aagcggggag ggccctggta cctctgatag ctgcaccagg tggtttggac tctatcctat 151020gagctgggaa gtcattaaac ggagccccat gagcagatct gctttttggc ctctcagaag 151080gggacacacg gggccaaggg tggggtcttg tttcccctgc ggtgggaggg caagtcatct 151140ctggggcgac agtgggaggt ttgaggctgt ggggggattc tggaagaacc aatgtggaga 151200acaaagtgag cacagggatt ggagaagcag cttcagggct attgaaaaga tgaatatttt 151260aaattcgtat catcagacat tatggaggtc cctagggatg tggcaaagca ctacacttac 151320gtaattgtgc ttcagaatgt cccttgcctt acctgagtta aacttagttg aattgagctg 151380ccttaattga actgaaagtg ccaataaaaa tagagaacaa aaactgccaa aacaaattct 151440gtggttgctg gagcaccagc catcatcagt ctcatgacag ccaagactca gcagctccct 151500ggttgatttc acatatttat tcttgctttg aaatggaaag cctggaagag aagctaatta 151560ttaaagggaa tcaaggagtc aggcaggggt cggggggagg agatttatct gagctgttac 151620tttgctgcca ttgggatgcc acagtatctc aatcctagag ttggagggga gttaaacaca 151680gggcagggca ggatggggga ggcagcctac ccaggacgtg gctgtgggga cctaagcaga 151740tgtgttcctg catgcgttgc tcagtgagga actgaggctc agagagctcc agatggtggc 151800tagaaagtag gtctgtctga ctccaaatca gtggtcttcc tgccccagcc aggtgccact 151860caagcgagat gcagaggtgg tagcaggggc cctgccatgg ctggctgcgg cacgtggtac 151920acacaaggag gtggcagagg aggcttcatc acattggcca ttcctttgtt tattaaactc 151980cctttagatg gggagccctc cgtggggcta aaagtagaat taatctcacc ttctgaccat 152040ctctgtatct gttgctgcag atgagaaaca ccacgtaatg atttcgggag actagatata 152100ctcgccacgg caaggccaca attatgggcc tggtggacac ttcaggtggc aatttagtct 152160gtctgcatta ggccaggctt ctcttctagc tctgtgacgg ggctggctct cagggaagat 152220cccctggggg aggtaagacc atgcttataa gctcctgcca cacatgcagc tgtcaaagca 152280acccagatca cctcggagca ggcgcacgga acagctgagc acacgacttc tgctcctttg 152340ctcagagcaa tgacttctgg cttttattct ttgtccaggt atgtaccctc tggtcacagc 152400gacccaggat gctgacagca gccggaagct ggctcacttg ctgaacgccg tgaccgatgc 152460tttggtttgg gtgattgcca agagcggcat ctcctcccag cagcaatcca tgcgcctggc 152520taacctcctg atgctcctgt cccacgtcag gcatgcgagg tacgcgccct aaggagctgc 152580tctgcttggg cttgggatgg gattatgtgc tccacggagg gtgaagtgat ttgggaaaag 152640tgtctgcaag ttaaggaaaa tgaatgcctg aaagggaatg gggaatttgt cagttcacac 152700acctgtaagc aaagatgggc acagagtggg catggaagga atgtcatgtg gtatcttaca 152760ggctctgcat ggcagccagt ggtggctcat gggtttttca attgctgggg tttatagcct 152820gtttatggag tcctaaaagg ggcagttcct cccctaacac gaactgccac ccctgtttac 152880accacccagg gctgaggccc tgaggccact ttttgtggag aggctaagac ccgctcccct 152940agatggcccc tcgagctggt gatgcgaaga agtgcacaaa tgcttcccta agagttgttc 153000tttcggtggc atcaggaaat taaggataag acttaagaga agtggtggac ccagcagatt 153060taggaaggca gggctgtagg tagggcatgt ttctgatcag gaaacgtaat tgtgtgtgct 153120gatgaagagg gtgtgcagtg gtggctactg ttggtacaat gatgctcagt gcttggtgtc 153180acccacgatg agggtagcct tgccctggag ctggaggagg ggaggggagg gtggaaggta 153240attaactggt cactgaggag gcaagtctag aggctgtgga gaaggacaat atacacctcg 153300agaatcttaa gtgagatgaa gacctctgcc tttccccttt aatgattgct cagcacatag 153360ccatttgcag aacagatcct gtgtttgtag attccttcat tgtgaattta tctgcttgct 153420aaaatttatt tgtaacccca aaatcaatat ttgtggtgtt tttgaggtca tgaacagagt 153480ggcagaaatt ttgagttgcc ctttatgtac agtcccagct gagatggaac aagcagctgc 153540tctcatactg tcaacaagtg tcctttactt ggtctactta gtgccatggt tttacatttt 153600tgtgcttttg gtgacttcac tgtttaaaat gcccccctgg tgtggtgctg aagacctgtc 153660tagtgttcct cggtgtgaaa aagctgtgat gtgccttatg gagaaagtat gtgttaagct 153720ttgctcgggt gtgagttata gtgctgctgg ccatgagttc aatgttaatg agtcaatggt 153780atttatcaca taaggcatct ttagaaagaa acacacataa aacaaggttt tgtattgatc 153840agctgatgaa gatgtggcca gaggcttgca ggaacctaac cctgtatttc ccctatgagt 153900gaggattcag tgttcacagt gactttacgg aacataatta ccgcaaacaa tgaggattga 153960ttgtcctatg tgtcaggcca ttgtaggtgt gtggtgggac acagaggctg acaagacatc 154020gtccttgccc ttgagcctaa attatcaggg ggagctggat gcacgagcca tggataaatg 154080ggctggggga agagtgggtt taggggtggg gtagactggc tctgagcaaa gagagccggg 154140gaaggcttcg gggttcctgt ggctgcctcg gaggagggaa tctcagcacc tttttgtccc 154200catagtaaca agggcatgga acatctgctc aacatgaagt gcaaaaatgt ggtcccagtg 154260tatgacctgc tgctggagat gctgaatgcc cacgtgcttc gcgggtgcaa gtcctccatc 154320acggggtccg agtgcagccc ggcagaggac agtaaaagca aagagggctc ccagaaccca 154380cagtctcagt gacgcctggc cctgaggtga actggcccac agaggtcaca ggctgaagcg 154440tgaactccag tgtgtcagga gcctgggctt catctttctg ctgtgtggtc cctcatttgg 154500tgatggcagg cttggtcatg taccatcctt ccctccacct tcccaactct caggagtcgg 154560tgtgaggaag ccatagtttc ccttgttagc agagggcaca tttgaatgca gcgtttccac 154620actcaatggc ctcataggat ctcagtgtgg tctttcttac tttccttctt ccttcctccc 154680ctttgtgaaa catcttaaag gttttggaat gaatggtgga aatctgactt ggaagggctg 154740cgaatcagaa aggggagagg aagtgacacg cttacagaag tgggctaacc cttcttgtgt 154800ggcacacact acccttccct ctgagagttg acctttgctg ttttccggac cactccattg 154860taagattgaa aacccctgtg gcaattgcgt acttacctcc caggcctgtg gggactgatc 154920atatcatatg atgcttattc tgtcaaaggc cagagggact gtggttaagc tgggatgtga 154980gtcatgttct ctccctgacc ttgctgccag ctgcacacag atttgtccct ctcgatttgt 155040attcacagag cctgccaata atttggggta tgtgtgtatg agcgtgtgat cattttcatg 155100caggactgtg ggagatacaa atctcgctgc ttctggagct gctcttcctt aaacctgttg 155160tcccatgggg ccagcgtggg tgctggagaa aggccgtgtt tgcaggaatg gggttctctc 155220ctgtgggtgt gggtgacagc cacagtgttt ccctggggca atgtggatgc agtttccatc 155280ttgtacaacc tcataagtag cagccacaat tgccccatca gtcaccacaa gtagtcaggg 155340atactttggg ctgtggatgt gtgcagtgtg ctgttttatg gatggatgag tagctatgca 155400ccccagtgtg tcagctctgg ggccacactg tatagccttg atgagtacgc cccttgaaca 155460agacccagtt tgtgaactct ccttaaagag aaatatttag ggataattat ttatagcaag 155520aaagaattct tttacacttg agagctcttt taaaaatatt ttcttattgg aaaatttata 155580tggtgggcag ggtgaaaaag aaacagtaaa aatattagtt cttattccaa gtggaacata 155640aataggacat gaagaagggc acctctgaaa tgacaacttt aactcacctt ttaaaagatg 155700tgaaatttcc agttttggat acacggtgaa tatgtaaaat gagtaacagc atactatgga 155760agccagcaat taaataatca tgtttcatta ttgcagtaac gttttaaaca attaccttgt 155820gatatgatat taaatatatt ttctttttga aaatatgttc actttgggta gcacatcctg 155880tatttactaa gtcattagga agactgcatt cagtgttacc aagactggtt tttgctagta 155940agacctcgaa taatccataa ttttgatatt ggtgcaattt tactataagt tgagcttagc 156000tgtttcagaa atgcttggac aagtacctag agaacacact gatgtctgtg ttctgaggca 156060gtctgaagtt attcttagag actcagttac agctttagta agatttagta caggcaggat 156120aagcttggtt tcataggaac cagggaacca gtgttagtgt cagcttcttt cctcctggtc 156180agcctagaat cccccactcc caatagaggg gtttggaagc tggagagtag gaagtaagag 156240gcaaagaagg cagccttcag caactcatta tctgccagtg aaattctatt aaatgtattt 156300ttaaaagaga ttaccaggta acaaaaacat aaaaaaccaa aacaaggcca gatgtggtgg 156360ctcacgcctg taatcccagc actttgggag gccgaggtgg gcgaaccact tgagcccatg 156420agtttgactc caggctgggc aacatggaaa ccctgtccta caaaagatac aaaaattagc 156480caggcgtggt ggtgcaggcc tgtagttcca gctacctggg aggctgaggt gggaggatca 156540cctgagcctg gggagatcaa ggctgcagtc cattgcactc cagcctgggt gacagaggga 156600gaccctgtct caaaaaaaaa aaaaaaaaaa ttgccacgaa atatatatat atatatatat 156660ataatttttt ttttttgaga gtagatctta agacagagat cacttctact cctgggagtg 156720aactggcaat ggcaatccct ttagagcctc gagtgggcag tatcaggagc gccgcacagt 156780gagtttccag ctgagctatt ctcaccgaat ctcgctctgt tctcacagca cccctctgtc 156840aggcctgtct catagtgact gcccaccagg actgactaca aaagacttga ccctaaaata 156900gtcttgaagg gatttttctc aaaaaattaa ggcgggaaca caagacaaag ctgtcagcct 156960agtcacaaat ctgaagactc aactgcatta aaaatagtgc aaaatcggca ggagctgtac 157020agtgcgagtc ttggtctgga atactccccc tgctaactca gctggaaggg caactatctt 157080agatttcagt aaggaagaaa aatcagttac caatacttgg cagagccata ttatatatcc 157140atatatattt atgtatataa gtggaattga agcaattcta gaattttcta gcatgtgaaa 157200gcagggttta gttcttattt acgtctgcta agggactttt caaattcaaa gtgaaccttc 157260tgtttatagg cctattttga aacaaagtat cctcacttaa taagatttga cacctttttt 157320tttttttttt tgagacaggg tctgactcct gttgctcagg ctagagtgca gtggcgccat 157380catggctcac tgcagcctcg acctcctggg ctcaagcgat cctcccacca cagtgcccca 157440tcccacccca ttcccgccct cgccgagtag ctggggtgca caccaccact cctggctaat 157500tcttttaata tttgtagaga tggggtttta ctatgctccc caggctggtc ctgaactcct 157560gggctccagc gatctgcctg ccaaggcctc ccaaagtgct gagattacag gcatgagcca 157620ctgtgcccag ccccgccaca ttttttttta agttgctgaa aatcttttaa aaagataaaa 157680acacattatt tagtatctaa agataatatc tgtgccagac acagttctca gtgcctcaga 157740cattcacatt taatccttat tataataact gctatttcct tattttctgg ttgtggaact 157800agacacggtc taagcaaact tgctgaaggt cacgtgggga gtaggtgatt gagctgaaca 157860caggcagtcc aagtccagtg ctgacagtga ccatgcactt caaacagttt aaaaatttaa 157920agaaaaatat tttaaaactg cagaatctat caggtgcaac ctgacatgca cggctgctgt 157980gatttaaatg gggccccctt gtgatacccc cttacctccc accacaatgt ccagaacacc 158040cctacagaca cagtaagttt gtaaacctct cacatcaaag ttcaactcca cctttcatat 158100ctgtgtaaat taaagcccac gggggcaaat tcacctattc aaggtcataa aactactcat 158160ggcaaagctt ggactggcac gcaagtcttc tgcttgccta gcgggccagt attgctcctg 158220ccccaggact tgcttctgtg agaatctgct ttgtgagctg agtcgcagca gaatggaggg 158280gcggtgaagt tagggttgtc ttctgctgta cctttagatc ccatctcctc agcttagatg 158340ggtctgcatg agcctttaca caacagcagc aatgacagat ggaaaaataa gatgcataat 158400ctgttattcc cattgtccca tctcaggttc atgagctcta gtgggtactg tgatcacctc 158460ctgtctgtga ctgctttccc caaacacgtg gaatatgttc cttggaagtg tactcatgta 158520aaattcacat cttttaggca ctgctgcttc cctgtggagt gtgatatact acagtgtgaa 158580aacacgtgcc acttattctt tatagctctc aaacttgctg gaattttggc tccagtggca 158640gctcttaaga tgtgcattgt ctgtgatgta tgatcgtagt gccatttttg ttgctttgga 158700gtcagggagg ttttttgttt gtttgtttgt ttgtttttta attccgagga tcctattcac 158760ttgtagggcc agccactggt aaactggtgg tgggtttcct ctatgggaag cacataagga 158820gtggtgatac cagccgcgaa cagttcctgt taactgtaca atggatgttt ttgcatttgt 158880ttcctctgtt gggtgtctaa atgccttaac tgttggtcct ataccttttg tcattcaatg 158940tgtacttcag agcctgttgg ttggctataa tttgccattt tctcagacga atgctttgta 159000tcattacact aatttgttga cttcatttgc aggctttaca tttgggcctt gtagaaatga 159060atgtttgctg ctctgtgaaa gcagattttg agacctgctt tcccttcctc cagggagtgt 159120tttccttact gtgtcccttt aatgtctatg gcactgtcgt agagagttta acatgatata 159180aataaagtgt ttcattattt tggctttaaa aatgtatttg ttgggggttg agtgtaagaa 159240cttacagtaa ttaggctaag tagtgtctac attctattct gaattcttat tgtggggtta 159300gagagtcctt tgagaatttg atgaaaacca gggctagtct tcctgggaaa gggcacctga 159360acacaaatgc ttgagtacaa tttcagaaga gttaagaagc tctgctttaa tgtatcttct 159420taaaaagaac aatttcatct ttagtcagct aatctcacac ttgtgattga tttatgacca 159480caggtcctgt gtatacaagt aaaatgcagc tcacaaaagt cctggtatcc agtgcatcga 159540ttatttggat agattttctg taatcattct gagtttgatt agaattatat cctttacaga 159600tggggagaaa agcaattcat tcatttgaag ttatcttagt gccaagagtc atgtgaaaat 159660gtcccttgca tgtgggcaat gaaagatttg cagacgatat aaaacccaga ctacctcata 159720aaagagtttt gggaatacac tgagctttga gtgaaagaag ctgcagtggc ctccctggag 159780atggggagca aaccagctta aaggccctta tcctgaggaa gagacaaaaa ttgacatgca 159840caatattaag ctttgaaatg cagaccacac ttcctttcac tgcaactttg acttgtcccg 159900catctctact taagggcaga aaaggcctct caaacactca cctcatttgg aatgaagatg 159960gagactcttt tgcctgaagc aacgatggag cagtgaccct ctaatcaact cggtggccta 160020aagaaaaatc ttgggtaaca ttttcacttc agtttccctc tgggatcatt gtaatccatg 160080aaaaaaataa ttttaaagaa agagttaaaa tactttgaag ttagttatgt ggttaaaaac 160140caccttcctt tctattatca atccaacaat ttgataactg taaacgctaa agtgaagacg 160200gattctcttc agatggtctc cttaactgcc cagggcttgc agatgtctca cccatgaggg 160260gcaccaatgt agaaagctga ggcttcatct actgatgagc ttcactggtt tcccctgagg 160320tttgtgcttt ggcagagaag gggaggaggg gactgggatt gtgtggtcag ctgtgcctgc 160380caacagatgc aggttaggaa ctgtgttcag tatcttccaa taagaaaggg gaaatgccga 160440tgcctatcct ctttgtttag gtagaaagta aaatgctact ggacttaaat gggcaacaag 160500gggctttgcc tgttcatttg ccatggagag ggctgggaat ccaggtgcgg tggctcacac 160560ctgtaatccc aacactttgg gaggccgagg tgggcagatc agttgaggtc aggagtttga 160620aaccagcctg gccaacatgg cgaaaccccg tctctattaa aaatataata attagccagg 160680catggtggtg tgtgcttgta atcccagcta ctcaggaggc tgaggcatga gaatggcttg 160740aacctggaag gcaaaggttg cagtgagccg agattgggcc accgcactcc agcctgggtg 160800actgacagag tgagactctg tcaaaaaaaa gagtagagta aactgggtat aagatccttc 160860cctttgcgtc cacctctcat gccatgctgc ctttgccatt ccctacaata gctgagggtc 160920acacgctgaa taatttaatt tacacataca cgagggtcca gagctaagtt aattctgtaa 160980ataagactta gaataaaagg ccctctccaa atattttaaa aataataatt tttgtttttt 161040ggaagattaa gcataccact gaactgcttt gttacagaat tcagtacaac agaagtctgg 161100ctaattttgt tttttaatga gaaacatctg agttgtacat atcacaaaca gcttcaagtt 161160tctgtaccaa ccccccgccc ccacccccgc cgtggccaaa cagttaaaac ccaaagcaaa 161220gcatcacttt ggatgtgaaa aagtcttaga aaattaactt acaaaaacat ccctatcaag 161280tcggtagttt ggcatttact ttacattagt caaaagctcc agctaaaatc taattttttt 161340aaaaaaaaat cgaagtttac attattcata cagattgggc attgttaaaa aatatgcaca 161400aataaccaca tccatgcaat acaatttctt taaaaattta aagcaatata aaagagcaga 161460gctaggtact gaacagaaca ttttggtgta taaccggcag ctcaaaattg ccagctgatt 161520ggagtaaaac tgattctaag cgtattaaat atgattgatt gtttccatca gctaagggtg 161580cctatgagtt tctgaaccat ttctagggtg gaatgtcctc gcttgcttct ataatatatg 161640tgatggacac cactgctcat tgaccatacc tacattataa taatgctgtt ttacaaacaa 161700accagaattc acaaagtgct tggctcttca ggaaactgac atttccagag atccctaaac 161760taatcaacta gttctgccaa aatacccggg gcacctgcca cacaggttcc ctgctcctgg 161820ggaggaacac aatctgaaag ctgccctggg ctccagggag cccgtgctgg gtaagcccag 161880aagaagtctg cacaggtccc gggaccttgc caacactaag tcactcagat tggtctgggg 161940ccacgtgctg ggcacccttg gcaatcaggc aggtggtgta gcactgtggc cagctatgcc 162000ctctatgtgg ggggtggccc attggtgtac ctcagcatgg ggtaaaagga ccgggcaaag 162060ttgttggcct gagtgcagct gtagtcttct tcggaggagg gcagcaggca ggccaggagc 162120agcagcagca ggaggagcag ctgcaggggt agggctgccc ggaccaccct tgagaggaag 162180gagcgctgtg gccgtgtgct gccggggacc ctgccaacag aggaggttga gagctgattg 162240ggaggctcca caggcacaac ccactctatt acctaagccc ctgcttatgt aagtaagaaa 162300tccaagacct gagatttaaa tagggccaac agttggggtt cagtttcaga ggagaaaacc 162360agccctttcc agacaaaaga aaaccagatt tttgcaagga ccttgatagt ggcattggca 162420agactgagtc agtgggagtg tggagcaggg gaacgcactg ctgtcacggt aaagccccgt 162480tacctgctct ctgtctcctc ctcgccttct gtagttctca ctgctctgaa ctgctgggta 162540aagaaactcc agttagtaag ttgaagacag tttagtccta tcaatgataa aaaaaaaaaa 162600aatcctccct taaattatat accaccttta tgttgtgtta cagccaaact ttggagacta 162660gagtaataca attgagatta aacgtcacct gaagtaggaa ataattaggt taatctactc 162720agtttcaggg tcaagtgtgt tgaagttttt aatggcaaaa tcagggaacc cctttagcga 162780cactataaga gctctcattc acaacctact gtgatcccaa agaagagtga ctagaggcag 162840actgtaagcc tctctatggg tcagcagaga cctgtgctgt cctgaaatgg ctaatgggct 162900cttggaatcc caagcttccc tcatcttagt gactttaaaa aataaccagt gaggttctca 162960cagaaggaag gggcttctta cctttgctcg gggagctggc acggatgttg caggaggctg 163020gtcccccgag tctacctcgt cgaagctggg caggggtgag gctgggttct gaaattcaga 163080ccccaaaggt tgagcacaga aatgtgtttc atgcatattc ttcatgatat agctcaccct 163140cccctacctt aaatcaatct ttttttgttt tgttttgttt tagacagtct cactgtgtca 163200cccaggctgg agtgcagtgg cacaatcaca ggtcactgca gcctcaacct cccaaactca 163260atccttctac ctcagcctcc tgagtagctg gaactatagg catgcaccac cacacctggt 163320tttgtatttt tttttttttt tttttttgca gagacgggat ttcaccatgt tgcccaggct 163380ggtctcaaac tcctggactt aagtgatcca cctgccttgg cctccccaag tgctgggatt 163440ataggcatta gccactgtgc ccagccttaa atctttcctg aaggggctct tctattgctc 163500tcactcccaa caacaacaga ctttttcgga caaaaaggaa tgtaaacaag gaaaagccaa 163560cccatattaa aaaccaaccc atagcacaga gctggggaga ctgagtttag cagcagcagt 163620ttgagtagat ggtgagctca tcacaggtcg gtgatgagat catgtggcca tcaagagagt 163680ctagttttgg ctgcttagga gaaaacagac ctgccctaag tctggtgagg ccacagtctg 163740ggtattccct ttgttttgag ttggatccca ttttaaagta ggccagctat ggtggctcat 163800gcctgtaatg ccagaacttt gcattgcttg aggtcaggag ttcaagacca gcctgggcag 163860catggcaaga acccttctct acaaaaaata caaaaattag ccacttcctt taaaaaaagt 163920ttaaaaattg tcatcccgcc actccctttt aaaaatgaca aaacagattc aaagagctca 163980tgcagctctt taagtccaca cagctagaaa aagggcacga catgaggccc cactcggacc 164040acctggccct tgcttctggc ctctgtctta gagcattgct acaacactgc tgtcctgtct 164100gcatgtaata cggcaatctt tacagttaaa agctacaagt agactcacct gggttccctg 164160caaggccatt aaatcttggg acacttgctc ccgtaactgt ttgagtttct tctcaataac 164220atgcaccttt tcttcagctt caatacagtc ttctccatgt cccttaatga gaaggctgtt 164280tgaaatctcc tgtaacatgt ccacttgagg ttgacgttct accagctcct tttccagttg 164340ctgaaaacag ataaagtgtg tgaaacagtg acctgtccaa agggaagcga gtgtggacac 164400aggaggtttt tctcagtacc agaaaaattc cagaatgaat gatgggtagt actcaactcg 164460agaatcctct atttctcata tctgcctgtg tcctccagcc cagcactatt tagatagcaa 164520gtgtattatg ccctgcccct atttttaatt tcttttcact ctgaagacaa ttgtgatgct 164580ggagaaatta gaagaaagaa gtgaggcaag tggtgtggga agaccaggtc cctcatccac 164640cacggaagga agtaaaggga aactctaaag tcaatcaacc gagaaacagc agcgtaacca 164700tagaggttca taccaggcaa acgggcttta attgttaagg gtggttatct tgcttgcaat 164760atggccacta gacacatgtg gctattcatt tatttatttt tttgaggtgg agtctcactc 164820tgtcgcccag ggtggagtac agtggcacga tctgggctca ctgcaacctc cgcctcctgg 164880gttcaagcga ttctcgtgcc tcagcctccc aggtagctgg gattacaggc gcctgccacc 164940atgcccagct aatttttctt gtatttttat tttattttgt atttttagta gagacagggt 165000ttcaccatgt tggccagact ggtcttgaac tcctgacctc aggcgatccg cccgtctcag 165060gctcccaaag tgctgggagc caccgtgccc ggcctacatg tggctattta aattaactaa 165120aattaaaaat tcagtttcag tcataatagc cacatttcag gtgctttaag ccacatgagg 165180ttattagaca gtgcagacac aggtcatctc tatcacttca gaaagttcta ctggacgcca 165240ctgctttgct ttgcaaacat tttaggaagc tgtattttta aacaatggct agcactctga 165300ttaaaattta aaatatttaa accacataca gatttatgga tgaaaatgtc accttgagca 165360ccagtgagaa tacagaaatc tcatcttggt gtgttagtga caaatacacc aggacacgac 165420tctcctccaa gaagaaagat ttttccatag tggatacaga aaacccattc agctcatcac 165480aggtgtgaag caaatgagca ccatatttct ccccaatgag caggattcac gagagaaaaa 165540tacttccact caaatggcct taagttcctc aggatatcga gagcacctat gtcctcagtt 165600ctgtggactg tggctctcca ttcctgcaag tcatttggct tcagaactac aaagggcagc 165660tcggtaaaca ccaactggag gaacactagg gctgaactcg tgcatttaca ggcaaacacc 165720actggcacag ttcctgaaat actaggcgag gctacctgag tgtgctgcct ctctggggct 165780gacagaggct ggtggcagta cagagccctt gggaggaaac ttaccattag ttccctccga 165840cactctagga gagcccgggg gtctgccttt ggatcggtga catgagcctt ctgcctccgg 165900ttcttggcac tcgctaacca cagcagcaga ttttgactca actggtggaa gtccttaaaa 165960aacaacacat cagagcgcgg ccgtgtctta gagccctgga agagggtgtg ccaggaacat 166020ggcttactgc ttccaatcca tcacagtcat ttgcttttct ggccttaccc actgaatacg 166080aggaccagct ttaaactaaa tgccagggcc ccaaaatgag gaagaggaaa gaaggaagac 166140actcagcaca gtgccaggag gccgcgaata aaagtgccac tatgaaggta gggctttggg 166200atgcccagct gattttatat atgaaggcca cacgcattcc tcagccaatc tgctgggttc 166260tctgctgggt tccagtggca gagggaaaca acccaacagg acaggctggg gtgggctcac 166320gttccctgag acacagccag agctaggcag tggtgcagag ctcacacccg ctgtgcattt 166380actgtgtcag gtactgggct aggattatac atactgcctc atttactcct aaaaagaacc 166440tttttaaggt tctttctaaa aagaaaatgg gaatgattcc cattttcaga tgagaacact 166500gaggtctagg aggttcaatg ctttgctcaa agtcacatgg ctattaagca gtaactgctc 166560ctctgcttca aggccaggtc tcttaatcac tctggcaaaa ggacaaaaag aggagttgtg 166620gggtggcatc tctaacctac aggtcgcctg caggaaccct gactccagga gtggctctgg 166680gcctgggcaa cagagacgag acctgggaca gggagtaggt ctctgagatg gccggtgagc 166740ctctgtggaa acaaatggta aagcacatgt ttcctgacac cagccacatt tggctgcacg 166800gaagcagcct gatgatgata aaaatatctg ctgctcctac cacgtgggcc tgggtaccgg 166860ctctgggagg cgggctgctg agtcagcgta cctggcactg catgagcgac tgtcgtaagc 166920cccctctcca gctgtccact gcgccctgtg ctgcttccca gagcaggctc agctggcgga 166980gtctactttg gagctctgtg gattcggggc tctcggtttg cagaaattcc ttgctgctca 167040cgttgacaga gaccactaat gccttgtaag tgtcaaaggc tttcagtatc tcctaccaga 167100gagagaaaag atgaggttag gagctctgaa agtagggagg aggggctcat tttgaacctc 167160agtagggaac aaattttcca cacacttttc caagctgcgg ccacagaaat gagagtgacc 167220tcgcctggga ctggctggaa agcatcaggt gagggagctg ccttaaggtc tgtgaacttg 167280gccaacaggg ccccaaagta gaggcctgct ggagggagga gaccctgctg acctctacag 167340gccccgctct gcaactggct taatgtttct ggaccgacta aaggtcttcc agataaagta 167400tatgtggtta ttctgcattt gctcctagaa atgccagaaa tgttattcct taggaggaat 167460ggtaacccct aatatattaa cacagatagt aaaataaaag tacaaatatg aaaggtacct 167520ggatcctcca aaacccctgc cctgggtctc aggctactgc tcatggcacc cttaaagcgc 167580agggctcaaa ctgtcccttt gcagctccct gggctagttc cagtggggtc aggacttatg 167640taagtgacac atcctaattg cataaaaggg taatgaggtc aggccacggg attcatcagt 167700aggtgagccg gtcctttccc aagccctttc aaggaagatt ccaccactag tctttcgtgt 167760aactgtgcag catacagttt ggaagagtgg ttttacactg cctgacagtg tccactagca 167820cctccagaaa agtctagaaa tgcctcggat gtcagatttt tccatctctg gagatctgag 167880ccacacctcc ccagtactct gggatcagga tcatttgcag atactcttcc atgtgaaaac 167940aaaaatctgc atctatgtcc ttgccaacta tggaactgaa atggaagaga gtgacctcta 168000catacaccat ctgcaagctg cgcctatgcc acgaatacat gggatctgct cgtccagcat 168060agcaatgcgt tcacatgcac attctgaatc cctcttcccc accacacctc taactcacct 168120gcagtctctt cactctcagt tctatttcct ggatatcaga gggaggcttt gccatcttta 168180acatttccag ctctgcttca gtttttttca gccaagtagt gatggcgctg atatcagagt 168240tcagctgttg caaattttgt tttattttga gcttattgtg aagctcctgt gcttgaatca 168300tctcccatct gtcgaaggca cctggaatta aaaaatcgcc aattaaaaaa agcaataatg 168360caacagattt ctctgcctcc agctaattag gggtcagggt ggttaaaaaa aaaaaaaaaa 168420aaaacaaaaa aactgtaggg gattcccctg acagaagggt ctggttttgg gagttctctc 168480ttctcctgga atgtggaata gcacatgatc tgaggcgtac actcccacag aggctggaat 168540gaagtggaag gtctatgtgc tacagtttca agctcaacag ttcagttcct gctgaatttt 168600ttcagttttc tcctttggtc cacaaatgac caggtcgatg ctgtcagctt tagatttagt 168660tgagtaatta cttttataat tactgggctg gtcgaatgaa gagacacttg agataacact 168720gatcatcatc ctcaagtctg atctttacac acaaatatag aaagtgaaaa cctgcacttc 168780ctcaggaggc ttattttacc aacatttaaa gaatagtttt caaattacac tgaacagcct 168840aggaatttgt tcgtgaatgc caatcgctga tttaaaaata acattggttg ttgtagggta 168900agtcccccag ttctggcgaa tcgtatagtt ggttaatatc taatgaatgt tctcttgagc 168960aaggacttaa ataccatact ctgcctctct ggacgactgc cagttacagg cagtacctga 169020ctgctgctct gtgataccgg ccagtccccc gtcttcctgc tgtgggttgc cattcaggac 169080tcgcgggcct tctttgccac catccgtgcc tggaggtaat agtagctttc cctagaatga 169140gccagttttt aagttaccat ctgtagatat gaaggatatc tgaagaacaa ctggattcat 169200ttcatattaa cagtgaagaa atgagatggg cccctttcct ttgaagggta tcttgctagg 169260aatacagtgg tgctttagtg tctaggtagc atttataagc tggcaaagct gatggcagaa 169320ataaagcctc agctaaggcc cagggagagg gaagcagatg agatgtcagt gatgggaaga 169380aaagggtctg tttcaattgg catgcttcca aacacccctc aaatgatcca cacgaaacaa 169440atcagaacaa tttgttgagc ttaccagacg gttactgcgt tagcactttg aattttcata 169500gtgttttaca gaattttagt tatccctgtc actctgtaga ggtggttggc atcattatca 169560cagaatcaga aatgcacaga ggatgaatct gcctcccagg ggcattcgtt accaagtgtt 169620tatctttcac cgcacatact gactcttctg ttatcacaca gccgattggg ctgttatctc 169680gggcctctcc aaaaataaat gtgtcccttt tagaaaagcc ctgctttctt tgtctttctt 169740ctgtctgtat ggcctatttg tgcagaatga aaataataaa aaaaaaacct tcagatttgt 169800ggtgatacat ctggcttcag acacaacaaa agggttgaga ttttgaaacc ggccaggtga 169860ggaacacagg cgtcatcaca ggaagggata gccaagccac atgagccaag gatgtaaagc 169920agaggtcagg gactgcggtc tttcccctgt cttattattc tttttaaaaa tgacacctgc 169980ttgttaagca agaggaagtg tttctgaatt ggacacaaac agcccattat cagctgactt 170040ggatttgagc atgcaaaggc agaatctcct ccaatgatgt aacaatattc tctccttaat 170100ttctctcgct gctgccctag gctgccctcc caggagctgc ctagccagag catgccaaga 170160actcaaggca gctgagggaa atgtaactaa gaagaaacag ccattgctat aaacaacaaa 170220ccaatacagg aataataatc acgaccaata caggaataat aatcacgacc attacttctg 170280aggaccttct tttaccttta tacacaataa tcttaacaac tactaggtga aatagcttta 170340gcctcaggtg agaaaggctt acagaagcaa ggcgaagtgc ctcaggtacc atggcaatgg 170400attggcaggg ctaggaatca aatccgtgtc cactgcagca caccatcaat gattttttgg 170460aataggatgt atggacaaaa ggttaacata acaaaggctt gaaggttgga tgcagtggac 170520catgcctgta atcccagcgc tttgggaggc tgaggcaggt ggatcacttg aggtcaggag 170580ttcaagacca gcctggccaa catggtgaaa cccccatctc tactaaaaat acaaaaatta 170640gccaggcatg gtggtgcaca cctgtaatcg cagctacttg ggaggctgag gcaggaaaat 170700cacttgaacc caggaggcag agattgcagt aagtcaagat cgtgccagtg cactccagcc 170760tgggtgacag aatgagactc cgtctccaca acaacaacaa aaagacaaag gcttgagagc 170820tcaggagcag tcaggaaagc cagcaccttg cttgctcact atttgcacag acacctgact 170880cgacagtaat aatgactgtt acaccacacc aacatacaga aagatggttt ttggccagaa 170940cctatctcta agtctaatcg gttttgccgg ggttctaatg aagtatggac agctcaacag 171000agacactgtc accataagaa aaccaactca aaatatgcct ggggaatgaa tacacgcagt 171060ttacaccaaa atctctctct cttgcaaatg taagtatgac tcacagacat aggctgtact 171120cccagtgaag ttaagactta catagggtgg tttataaggg gtgctggacg cagggggaac 171180aggtggaaca ttcctgtcac cttccatttg cttgtagtga tgctcgggac aggaagggct 171240gtcgggaaca tgccacgagt ggccatccga aatggattta cctgaaacga aagacagcaa 171300gcgacagtct attctctcct actgaacggg tcacgggtga catggcatca ggttacagta 171360tgaaaatgag ttctgcagct ttccttgcaa agcatgcatg tttgcaaaat gtcagtatgt 171420cccagaagag aatgagatga gtcatgcaac tcagcaacac tgtgccttca ctagatgggt 171480acacaacgga gcacgaccaa catgatttgc tgtgggatga gtggcgtttt ggagccaggg 171540tctcgagtgg tagggacact gagtggcaag gccgggtctg gggaccaaga tggcagaagg 171600cctctcagga gtgaggggaa gagtgaacat caagatggac ttgggaacca aaaggctcct 171660cagggctctc cccgacccac catggcagat agggatgggg ctaaggatga ggattagagc 171720tgcaaatcaa ggaaaggaag gttttttccc tcctgtggca aatcatcagc accctttacc 171780gccccaaccc cccacccctt tccaccaggg gatcccagta aagcaaggaa acagttccaa 171840accaagccac catgaagaaa gcttggccca tgccaaagga cttcagttag atggagaaaa 171900aaatcatcaa aacaggcacc atgaaaaatt ccctctgcac aggcctgaag ggaggagagg 171960gggttggcat cgagacggct ctggcttgga tggggttttg taaggagcac gcagttatgg 172020gtgagtacgg cagtgtaaat acaccatgac acaaggacca aggcgcatgg tgtccacaga 172080aggcagaggt agacacgaca tgggagcgag gttgaggatg cagtgtgcgc cacaggatgc 172140tgccatgttg acacatgcat gggcgggggc ctaccagaag acgctttcaa agtttttgtg 172200gtcattttaa gatatgcctc aggattttca gggatttcta catctgaaaa agaacaatgt 172260catttgcctc actggaggtg cctgaagatt aatgcccaag tattaacaca cagtagaaac 172320attgtcagct ggaaggagaa aactaccctt tattttttta attaaaagga ttttgaaaac 172380cagaaagatt tttttttcca gccaactcct gactgtccaa agcaggacac tgggtatcaa 172440agggagaagc tcagtctgca gacactctga gaaactcaac tgaaagggag ggtaggcaca 172500gtggctcatg tctgcaatct cagtactttg ggaggctgag gcgggaggac ttcttgagcc 172560caggagttca agaccagctc tggcaacata gcaagacctt gtttctacaa aaaatttaaa 172620aactagctgg gtgtggtggc gtgtacctat agtcccagct actcaggagg ctgaggtgga 172680aggattgctt gagccctgga ggttgaggct gcaatgagcc gtggtcgcac cactgcacct 172740cagcctgggt gacagtgtga gaccctgtat caaaaaaaaa tcactgatga gtctcccaga 172800cagccagagc catcactgag taaccttagt gtctgaatgt gaatttcatt ttttttcccc 172860ctttcattcc acctgtgatg agagtgagcc aattctcagg taagttaaga gatgggcagg 172920atagagcaca ggaaaccaca gaagtcaggg tcggccacaa gatggactct tggtcattga 172980gagctggccg tgttgctaag gggctaaaaa catagccaaa cgggtcactt tccagggtgc 173040tgggaaccca gctgttacct gacagtgcgc tgtagtatgg gccctcctcg tcctcttcgt 173100gagaggagga gccccccacg tcgcctgtgt ggtcccactc cagggggatg gagtccacgc 173160tgacaggggt ctcgcagcca gaccgctcgt gccctggggc cactagatga cacagggact 173220gaggagatga cggttcctcg ctctctcccc gtttacgcca agaatcagtc tggatttctc 173280tggggtcttc catgtctgtt tcattctcag aggcctcctt ttcatcttcc aagccctaaa 173340ccacagtatc cagttgttag aattaatcaa ctcataggct tctgcaatta gaagagcatg 173400agaagtctgc ctatctagca gtttccaaaa ataactgcca atagtatttc taaggcagct 173460ttaaaaagat caggttccca ggtcctgttt ctggttattc cagtttagac aatctggagt 173520ggggcctaaa atctgttatt ttcccaaacc tccagctatt ttttcttagg aaaaaaaagc 173580tgaagtttga taatctaagt taaacaactt tatctaggag ataaggaaat ggtggccaaa 173640gtttcattga tggttaatgg caggttcctc caagaatcag actatttttg aaaaggacat 173700ttgaatgttc tgggtaagac tcaaacttca accagatgca ccaggttctt tctttcttat 173760cctccaactt aattgctcag tgtctgtatt ctgcttgtgt cataacgtct ctttcagtct 173820aagaaaaatg actctaagtg gctgtactta aaaatgagaa attgtggggt aatttttgag 173880gctgtgtctg atgaaataac tacatttgaa tgtggccagt tggagacaca aaaaaagaaa 173940gagtggccag tcgtatcggt caatggttgt cattcaagct tcaggcacac aaaggcagta 174000cgctttgaac agtggcctta actccacctg aacttcccaa aagggccttt cgtctgggga 174060aacatcaaag ttgcttttga aactcttgtg ttttctcttc caaggtgaga acaaagcatc 174120agaaaggcac aaatactaaa agcttccagc ctctaagaaa gagggaaggg caaatacatt 174180cctaagttac taatgtaata aaagccagaa gagttcttca agaattctag gggtagtgac 174240gcatgccaga caagcccagc cctgaggaat acttaaaagc gactcgtgga gcatgcgcct 174300cagaagtgcc atgctttcca ctggggaatg ggaacttgta agttactgtg gctctatatt 174360aataaaagtg gaaattaaaa tgttttgata tagccctttc ttttcaacca aaaaataaac 174420cataattaac cattataaga caggtcccct aattctttaa aaatttggtc tcacgtaaac 174480tctgagcagc ccaggtgcca tttctaggca gcagtgcccg taccggagtg caggaggtga 174540gccgccggtg gaaccgggag acccttccaa acacctcctg gcagtagcgg tggagttcct 174600ccagctcatc ctcaatcagc acagcatcca ggggctcgct cttctgaatc agctgctccc 174660caaacacaat gagctgatca atcttgttgg tatttaatgt aatttcctgt tggaagccct 174720gttgagagag gagaaagaaa ggatttcaaa gaaagttacc ctgagcttcc actgcctgtt 174780tcttctaaca gggccataga aaagcatcca aatgtcatta ttgttgtttt ccttcaccta 174840ctactctagc tttagttttt aactaagtga caatgacatt ttaagaattt taagtgattg 174900tctatattcc ccctcacggg cactggcttt tccaattaaa ataattctaa aagtttgaca 174960cgggtggaca tttactgtta ttaggtgtga gagaaagaac ttgccatggc aaaacttgtt 175020gggaacagaa catttcccat aataagccac gaatgggcat ttgcttttgc tgacacaata 175080atattcactg gtgctgttat actagggaag gcaaatttcc atttctcaac ctcttccaaa 175140atctggccaa ggagagaatt acagtgagat tagcctgtca tacagatatc agaaacaatg 175200ggagagcgga cctctgttct tggccccact ggtgacaccc atactcctgg accgtaaaga 175260acagtatcct tgcacgtgat aatctggtag tacacaggtg ggatgtagcc tggctgcttt 175320aagaattctg aaatttgctc tcagagtatc gtgtgaaatt tgcacttggc cagctctccc 175380acgcaaaggt gtgttctttt gagaagagct agggaagcag cagccctcac attcagttgg 175440cgcatcttgt catcggcgtc actctctgag aagtgctcca cgttggtcag ctgcaggtcc 175500atctctgtga gccacaccag aatgctctcc ctggtgccct caaattcttc cctctggttg 175560gtgaaatgct gcaaaagtgg ggaggaaatc acatttctac tgaccacgga acaagagttc 175620agacagacag caaggccacc ttcggccaca agaaaagggt acccagaata ctcagggaga 175680aatctgcctc tagggtaccc agaaagcccc agaagcacta tgttgttcag aggctggggt 175740ctggttctgg ctttgtgaga ccaaacaagc cccctcaact tctctgagcc ccagcttcta 175800cgctggcaag tcggggagag agtgggaatg ttgattatgg tctctgaggt ccttaagcat 175860ttgaaattct aaaattctct agtccaaatc agtgattttc ctctgtcctt tgaagagccc 175920cctgaggggc caggccaggc cttctgcctg ttttacacat tggccttctg cagcttttgc 175980ttgacggaag gatttagtag cttcaagatg gaaaagcctt gatatcagga ttgaggccag 176040cctggtgccc ccttctgcct tcactgatag aattctgaat gtgtcttccc agagaaagaa 176100gactcagagc agttgtatca tctccaatta aggatgcctc tcaggttact gcttcacaga 176160taggcagctg atgttcagat cagtatagct tcagtgccaa acgaaggcag attagccaca 176220gccagatcgg caggagataa ttaatgcaag aggccgggca tggtggctca cacccacgat 176280cccagcactt tgggaggcca aggtggaaga ttacttgagg ccaggagttt gagaccagcc 176340tgggcaacat agtgagaccc catttccaca aaaattaaaa tcagccaggc acagtgatgc 176400acgccggtaa tcctagttac tcaggaggct gaggcaggag gatcccttga gcctaggagt 176460ttgaggctgc agtgagctat gatctcacca ctgcactcca gcctgggcaa cagagtgaga 176520ccctgtcaag aaagaagaaa gagagaagag agagaaagaa agacggagag acaggaaagg 176580agagagagac aggaagggag agagagacag aaagaaatgc aagggagttg tctaggttac 176640tagaaaagcc aatgtcttcc tgagaaggag ggagggggag agagagagaa aagaaaggaa 176700gaaaagaaag aaagcccaga tttacatctt tctagaatgg gttcaaccat gctctgccct 176760gagaacagac gcagaaatta aaattgtgtc ctgtagtccc tgcctgtgac agtcctttca 176820gtttataact ggattcacac aggtttacac agcacatctt ctgaatgaca gaaatctaag 176880gtgaatattt accttcaaaa accaaacctt tgtgtgctct gaaattcaga gaataatcac 176940agtccccaac cccccagtgc agacaggggc tcttgcattc acgtgccttg tccatcctcc 177000tcagtgcgag agctgggggg ggtcccataa aaacagacag atgggcaaag cggggctcct 177060tccaggggcc cacgtgttgc cttttccaga cccagcgaag cggcgccctg gactctgagc 177120taacatgcac accaccacgg gttacagaag ttgtaaaaag agctgtgcgt tggtaacgca 177180aatctcatca acaggccctg cctaagaagc cccacatgca aatccccctt ttaaaaaacg 177240aacgttggca gctttgggct tctttgtgtt ggaggactgt taaaacagct caggcctgaa 177300gtgaaacctt ctacctgctg ctttgtgcca caagcctgcc attgcgtatc gtgcgttatg 177360tctcctggaa aatctacacc cgagccaggt gcagaggagc tcaccctgag tctccgcagg 177420acggctgtga cccgcctctg aaggttgtcc cagcgctggt tgccctcgtg gaccatctgc 177480ttcagcctgc tggccgtgtc tgtgcggttc tcccgggcca gccgccggta ctgcttgttg 177540atgagctcca gctgagtgag ccgctcatga atctgccgct gaaaggcctg catcacatgg 177600gaggggtcag agccaaagca tgcagagtgc caggacgccc tccttccctt ctacatccca 177660agcgacctgc tgccaggggt tcatccccaa actgcaatgg cagcagaggg cctaattcac 177720cagtcctgat gccagctaca gaacaggctt tcaagttcat tacatagcag ctggagtaca 177780tcagcaatgt cttgcaaagc tgtctcagca gctgggggca ggctgtggtt taggcagccc 177840ttgccagagg tgaggggaag ctggctactt agaggaagtc atttcagcag cccccaggct 177900cgctgcaggc ttggttatga tcaggagacc gggatggaga aggtgtttac ctcaaacctc 177960ttcagttcct ctttggcact cgtgtacaac acctctgagg aatttgggca ggctgccgtc 178020ctctcagctg acttgagcca gtcctcaaag cgagaatagt cgtctaaaaa cttctgccac 178080aggcgccacg tctcctcgat tctgatggga gcaaagcaac ttaacacaag ccatcccctc 178140caagcctgtg cttttgcact tcttttatcc cctgacaccc gcacggttta gtgggggaga 178200ccctgttacc ccttctggga aataagaggg acgccacttc ttgtctgtgc tcctcctgcc 178260tgggagcgcc aagcagctca gagctcctgc ttcacagagg ttaaaaaacc aggagacagg 178320gctgcctcca ctactgccca gctaggtcaa cggaagcaca gcggccagtc cacagccaca 178380ccccacacag gacagctggg gagagacatt actccggtta catttttctt gaataaatcc 178440tttgataagc gttgggcctt ctctgccaat acctggcgca tttctaagtg aaatgaacag 178500aatagttact gtgaatgagg tcagaagggt gtattgagtt cggctgacca cgctgaaggc 178560atttggggga gcttagttct tacttcatgc gccgctccat ggacatggca caaatgttcc 178620tccagcgtct gtccaggctc ctggtggtct gctggatcga gtcacactcg gtctcatttg 178680cacaggcatc ggagtcgtgc agtaggacgt cacagatgtt aaacacggac tccacccctg 178740cgctgtgttg ttcaatatct cgctgtagat cctgtgatta caacagagta agaaacctct 178800tgagatgttg gtggcagcag gagggtcagc atcaaaggac ctccgttcct ccctcccttc 178860tcccagctca ggggatactt ccctgggtgg atttccacca tgaacttggc agtacacatg 178920gaatccctct catctttgtg ctacccttgt gctgtcagca cctgacacaa agtttaaaca 178980ctggaaacat ctaggactta tgcgctgaac catgtgaggg gtgccacggt gatgggtgtc 179040ctctacgcac tgacaggtgg cgccattact gttgagagcc catcgcccca cagacttcgg 179100gtatgcatgg gatctactcc ataccaccca accccaaagg gctccagttt agacttggtt 179160tcagggaggt ctttagaaac tgagtctctt cagcaggaac tctcaatctt ttatcagcta 179220ccgaactctt caaacacaca ccaaattgtg catgcagcag tgtggatgcc cacagcttcc 179280ccgtgagccc attcaagggc ttcatgagcc caagggagaa attctggcag acagaccttg 179340cctccccaac tgataaagtc cagccaaagc tgtggtggct tggagagagg tggggtctct 179400ggagtcttgt cctttgatga tgatagtgat gcagttcccc aatttgggaa aaataacatt 179460tatgaagaga aaatgcatgg tcataggaaa aaactggaaa acctcttcac gaataaacat 179520ggcattttta gtcatcatca gataatattt ctgctgtacc ccatgggtgg gctgcgggga 179580gtgagagaga taaggaacct gttttgccat tctggaagag ttaggcatcc ctgatgtcca 179640aattgtgtga caagtgacat gagaccacag cattaatttt aatatccaaa ttacattgtg 179700agaaagcaaa cactaagtca aattctatct taattatttg ataatatacc aatttctaaa 179760atctacacta ctttaaccac tattaactag actaaatcca gttcccagga ctcaacaaat 179820ttcagttaat aagaggactg tactataaat ggtagctgtc accatctaca gaaaggcaat 179880ataagcttca ttccaggtca ctttcatatt taaataactt gagattacta ctatattact 179940agatgggacc actattttca tactctcttt tttttttttt ttgagacaag ggtttcactc 180000tgttatagtg gtgcgatctt ggctcacgga aacctctacc tccgggctca agtgatcctc 180060ccacatcagc ctcccaagca gctggccacc atactcagct aattttttgt atttttggta 180120gagatggggt tttaccatgt tgtccaggct ggcctcaaac ttctgagctc aagtgatcca 180180ccctccttgg cctcccaaag tgctgggatt acaggggtga gccaccacgc ccagccccta 180240ttctcttact tttcttcata taaatacaca attccgatca ttgagtgttt aggttgatat 180300aagaaaataa aatgctaacc cgccaactga attttaaatc aggcagttcc taacactgat 180360cccgagtaca aatgaacacc accaggttat attcagaaga gatgtgtttt ccacttggcc 180420accctgtgtg cactgtgaag aatgtgcagg gtgatttctt taaagctctg cagtgtgggt 180480agaatgcctg atatgctcga atggtgggag tctccctgga gtcaatttga ggactaactt 180540taaagttatt acatctaaat aaaattccag ttttaagaac tcttaccggt tcacagaact 180600gtcatgggcc cttctaaaat tagaagatac atatcccatg taaatactaa aataataaat 180660taggaaccct aatactggta ttaaaataaa taatactaat cattaataac tgaaaaatgc 180720ccatgacctc atctggctcc aggtgggtaa ggtctagttg aaaagccgag agaagttcta 180780gtttatggac aatatgaatg ctctgtattt taataaactc tgcaattatc ctacattatt 180840tattcagctt acattgaatc aaatggctaa agaaaactga ttaatattta gggtaaatgg 180900ccgtaagaaa tgaactctac aataatttat aaaagaccat caacatcttg gttaaaaaaa 180960aaaaagccca aacattttca aaacttttca aaaaacattt tcaaaaaatt tttaaaaacc 181020atgtaaaaca ccacacaatg gcctactagt taccttgtac aaaaaaaaaa taaataaagt 181080gatagactac attttaaaat gcagaccatt taagtgctag acaagaacat tttgcaagct 181140gaaataatcc taccgctagt aaccacggag acctaagtct gcacaagaat caggttttag 181200aaactgtcca aacctagaga aaaagtggat ttaagaaaca acataaaaca atgagaaaga 181260cttttgccaa cagttctaac aggctcctgt tcatttcgtt tttctgcccc tgggcaggtg 181320acacataacc ctgaggttgg ggaggaactt gggggccagg gggtacagag caagaggaga 181380aggggagaca tgaaactgga gaagagaatg cagccagaag aatgagaata aaaaggaacc 181440aaaaacagaa ggtaagggtg gctgaggaga agaaaaacag gctgaaaaga cagaaaatca 181500tatagcgtga agcccccagt gccagctcct gaatgtgtct gtgggagggg cttgggtagc 181560aagtgggaca ggatggtatt gggatggttc agggggtttg aggtgaagct caactggccc 181620acggcagggg ctattaatta gccagtggaa gggtaactaa caacaactcc aagccaccat 181680ctggcacccc acagagaaac aagcaaccaa ccaatgaaca aactaggaca gggatcttaa 181740agattcaatg aaagtggttc gtgatttaag tgtcacaaaa ttgccactac ttcctgtgca 181800ttttaaagat gatatattcc gtaggaataa cccccattac cttatactta aaggtgttct 181860atataagaaa ggtgaaacag aaaggactcc tatagggtct gacccctccc caaccaggta 181920tattacagtg aggatccggg gacatcatgg ctcttagcta cttttggggg ctgagggctt 181980ttgcagaagg caatactagg tgatgccatg tttttaggtc ttatttttgg aagcaattaa 182040tttattatat ttagtttgaa aagtaaacat aggtaaggtg gttgtttttt taacccagac 182100caaaaatggt cactaggtaa ggttttaaaa agtggcccat acataattct tttgtgtgac 182160acatttgttt ccagtgtttt ggatggggta cttaaggtct gagtttagca taatataaac 182220acactgtaac ttattctttt taacaaaaat actgaaagta tcctgagatc atctgtggta 182280ggcagaataa tgacaatgac accccccaac cccccaacaa agacatccag atcctaatcc 182340tggaatctgt gaatatgtta gattacacgg caaaggagaa ttacggttgc taatcagctg 182400accttgaaat agggagatta tcctggatta tcagggtggg cccactgtaa tcctaagggt 182460ccttaacagc agaagaggag tcagctagag ggagatgtga ctaggaagac agacacagag 182520agatgcaatg ttgacggcgt tgaaggcaga agaaggcgca tgtgagccaa gcaatgtggg 182580tggcctctaa aaaccaggaa aagcaaaaaa atggattctc cccagagctt ccagaaagga 182640gcgtaatcct gatgacaccc tgatcttagc ccagtgagat ccatttcaga tctctaacct 182700ccagatatat agtaaatttg cgtttttaag ctgctaaatc tgtaatttgt tacagcagta 182760gtagaaaact aacacagcat ttaacttctt tacaataatg gtagtcctga acaccaattc 182820cattaatttt ttttttttaa gtaaccaagg gatagaaatg tcactaaggt gatgctgaat 182880tctataaaga cagaattttc aaatcaacca gattgaaatt atgtgccatt gtaggatggg 182940aagaaataag gctaagagaa tagcagtggc tccaaactgt atgcttttgg gaacaaattc 183000tctgcaattt aatgttctgt cacacttcgg atttataaaa aattaacaca caatcaacag 183060cccctgcaaa atttagccag ccattcttta aggggaaaga tactgacagc ttccatataa 183120tctacctgaa aaaactcacc actcagggta aattgttatt tgcaaggcct tgccttttca 183180cagtctgttc aaagacattc atcctagtgg atcacacttg taagtaaaac tgatgtgttc 183240agtagctgtg actccaatga gagacgcatt aaaatctctg taatgtccat taaaattcca 183300taatacttat atcaatattc ttttctgcac aaagcaaaga cagagcctag atgacctcca 183360tgaaaaagac atgcttaaag ttccaaggct aagtggaaaa accagtattt ttaatttgta 183420gcattactaa atcatatcac tttgaaagcc agataacagg tatcttttaa acagtaattg 183480taatagcact tagaaaagtc aagagcaggg agggccaggc atggtggcgc ctgcctgcag 183540tgccatctac tcaggaggct ggggtgggag gatcacttga gcccagaagt taaagaccag 183600ccggggcaat atagtgagac tccatctctt aaaaaaatgt caacagtagt atcaaatatt 183660aaatctggtg aaaaaggttc acccccaatc cggtctccaa gcacctgtgg tatgtggtgt 183720gtggtggagg cttaacatga agaaaacaga ttcaagtctg agaatcatac tgcttctatg 183780gttgaaacag aatctgccca taggtctgag aaaaacaggt gaacaaaagc taaagaaaga 183840ataagataaa cttagctatt taaaaaaaaa gtaaagacat atccaggcat tacaagtgag 183900aatgagcatg tggccatgag accagggaag gcaggaaagt ggattcgata aaccagtgac 183960atcaaagagc acctgtcagg tgggtacaat gagaaacact ggcagaactc agcagctttc 184020aaactgccca atttccaggg ttggctcaga gcatgcatat ctgataacaa aaatgcacag 184080agcattgcag ctcttaagag gcttcctaaa ctattggtcc aagcatgttc cttatcacac 184140cttttcttgt tttaattttt taaaaaagct gtaccagaca cacagaatat aatcccataa 184200aaaccttcct tggttagaaa agttgctttt ctttcaattc attgcctttc aaaactatat 184260taacaaaaca caacaaagat cagttaaaaa aagacataat taaaaaaatc tcattaacca 184320ccaagattaa ggtttgcatc ttaaacagta ttcacacagt ttcacaaaaa gctacatgaa 184380tgtgggaggg agcactcaag agacagatat aagaacatgg aaaggtggtc agttttctct 184440ggaaaaagca aaccaatgtt atggaatgtg agcacgtgaa aacacataga tatacgggga 184500aggcctcagt cagtgcatat cgcggacacc gtactcttgc aagagctcat ttctgattgt 184560cccacctgct gctcagcgag cctcttctgg atctcttgat catcgcagac atcataaaca 184620acaggcttgg aaagctcaga ctcaattcga gccaaccagg tgcgaaggtt gctcatgttt 184680ttgtccaact gctgaataaa agcaaaggtc tccttcagct tcttcaccct acacatattt 184740gcagaaaaac aaaacgaaac aaaaaaaaac cagtgatgga aaacaaaagc ccaatactta 184800gtggtggaag aatgtgccag taagagcatt gcagggtaaa aaagaatcta ttttgctttg 184860attttatatc agaacaactc tcatgtcacc agatacgagt tctcagagaa tatgtgaagc 184920tttgcaaggc tgttgcctct gttgattttc aaaaggactg agagttaatc cacccatgca 184980aatggaagtc ctatcattcc cagagtgaga gcccctgaga cccagttcat cttcctgggg 185040ctccagaggt gagaagatat gccatttttt cctaagtgaa aataaccact cctgacttct 185100gcccagtttc tgccgtgggt atgcctgaga ctgcagcagc cggtgatcta ccctgggaga 185160ttaaagatgt tacacgcctg gttcacttgg tggtatttgg tccacaaggc ctctcatggg 185220accctcctct ctgagtaagc agagaacatg tgccaagccc taccgcttca gtatgagggg 185280ttcacccagc cgtatacatt gaaccagccc atgcgggtag attatggggc atctctgtcc 185340gtctaaaagg gagggggcaa aggaaatctg cacgcaagcg cagttctaaa tgtgatctcc 185400ctcgccagtc tgcctcagct cccatccggc ccaactcggc gtggagcaga tttgaaagca 185460cacgcatggc atgagtacaa cttggcgtgc acgtccacat gctgtcaagg ggacgtgatg 185520tagctgttgg gggaaaacaa tctttttctg acatatatac taaaataaaa tgctccaata 185580aaccgctttc cttaaagtcc taaagcagag cgaggtgagc caggatctgt cctggcgttc 185640tgtgggattt gtagttaatt tcagacattt aaaagtaatg gctccaatgg aaagttccca 185700cttatcaata cttacgttgg gagatgctgt cagaactgca ccccatagct gggagcacta 185760gagagataaa tgggtgcact gaccctaact ctcaggaagc attgtgctcc atgccaccat 185820cacctcttac ttgaccgaac aacctcctct cagcccccta gacttcctgc ccacctttgg 185880ttctgtagcc aaagtgtaag taagttctag aaggcaagtc tgatccacgc tcaaagcctt 185940caatagtccc tgccctaccc tgaaaaaccg gttcagcccc ttgatctctg tacctcaagc 186000attcaggggc cactctgcct ctaaccttgc actctctcag acactgtgca cccaccagga 186060cagggagtgc catggccagg ctgcagccgg ccagtttccg ccgcactcac agcacctccc 186120acagggcctg gtgggcagga agctctccac aaatgcttgg tgaatggaca aatacggaca 186180ggcacttcca tgggactgac ttctagttcc attaccagct acttttaaga tttagtgaaa 186240gtcctttcac ctcagccccc ttatttcaag tacacccgca aagctgctta agcttcaggg 186300aagtggtagc atcgggtgag gtgagggccg gaactccaga ggcaagcaga cctgggccca 186360agttccattc tcccatctaa gagctctgca ccctgcaatg tacctaaccg ctgactctga 186420gtttcttcat gatctgtaaa attatccacc ttgcaggtgg cggtgagctt gaaataagag 186480gctgcatgta aagtcagctg cacagagtga atgctggacc acatgatgct acaatcacgc 186540tttatttaca aggcattgga cctcacaaag ttctttcatc agtgttttat tctcaagggc 186600aagcttggaa gaaggacatg tggtctggag gtggaagaac acagactctg tggtcagggc 186660ctgggtttgc gtcctggcca tgtttaatgc catctataaa atggtgttaa cagtagtacc 186720ttccttcact gggtagctga ggggactgaa tgagatataa catgtgaaag gcagaatgcc 186780tcggagataa atgcccagca acttacagtt gtgataagtt tcaaggagcc atgttttcca 186840aattacattc ttactgcaaa caccacaagg atacgttccc ttcaaagcca cagatgattt 186900attaaaaaga aagtaacaga atccaggacc aggcctggat acagctgtaa cctagatcct 186960gccttcattc ctatggcttc agaggtacac cctcctcacc ccagtatctt gccaccttga 187020ttgccctggt tccaaaaaga acactccaga caactaccag ggtagctgag aacccctggc 187080actgtgggac aaggagtcct ccaggcagtt cctgagggac tgccctcctg gaaggctctg 187140ctggagaaga ggcccagcca cgagagcttc ccgggggtgg gggaagggct caggctcgtg 187200acctctgcac agctctcctg ccctccccac ttctccctct tcccagaatg aaatctccca 187260cggccttcat ctagcccctg accctattcc accctaagaa tctcagcctc tttcttattt 187320tgaaaagaag agggctagaa tccaagtatt tatttatata tatttttctt tttccttgtt 187380tcctctcttc taacttaaag caattaacac accggaagaa ttcaactggt taaatgaaag 187440gagctaacta gcaacacaac agcctgggta cagatccaca ggactgtggt gatcttgaac 187500tgttaaccct gggggttagg tgtgcaacac gcagaggggg tatggagaag gtgggggaat 187560aatttgcttg gtttccccag ggctgtggta tccaaaatgg ggtgctcaga acaaaccaat 187620ggggtgtagg aataaaatat gagactgtct actgtcatcc cattctttta aaatttctac 187680aatgtgtatg ctttaaaatt ataaacttaa ttattaaaat aaaacatcta tatcccacgc 187740tgatggccac atgtgctagg cctgttgtgc tccatgttag caactcatgt actttttttt 187800tttttttttt tttttgagac agagtctcta tcgcctacgc tggagtgcag tgctgcgaca 187860tgggctcgct gcaacctccg cctcccaggt tcaagtgatt ctcctgcctc agactcctga 187920gtagctggga ttacaggcac ctgccaccat gctcagctaa tttttgtatt tttactagag 187980acagggtttc cccatgttgg ccaggctggt ctcgaactcc tgacctcaag tgatccacct 188040gcctcaacct cccaaagcgc taggattaca ggcatcattt cctcttcata acaatgctag 188100gagataggta gtattattat ccctacttga cagatgagga aaccgaggca cagagagtaa 188160agagcttgcc caagtcacat ggtcaatagc ggaagcagga ttctaacctg ggtagtctgg 188220tctggagctg ctgttcttaa gcactaccca tcctctcaca taatcccaca cacactaaca 188280tgcacatact ggagatacag gatcattttg ctgcagtcag agaacatgac cagaagtctg 188340gactcatagc tcaggataca ctgaggggta ggagctgata tagtagatgc tacaacctgg 188400gctcttagca ggtgcctggg gaggaggctg gaaggctcaa tacatgctgc ccacgtttcc 188460cctccatgat tttctttcag taaacactta accatttgcc ttgtcttgga tcctgcacag 188520accctgagga ttcagagata gctggaagat aggacttctt cccagagagg tcatcaccta 188580atttagatgt aatgttgaca ctgtaataca acataggagt tgctcaataa tagctgttga 188640aggagtaaat gatgttcact cataccttcc aatttgtctg atttgtcttc ttcataaaga 188700ggtggccaaa ttagattttt aaagaaaaga ggtaaagacc agagcatatc agagcccttc 188760tgccacaaag cagtgtgctg ccagcctagg agccactcgt gctcctcagc cctggtttca 188820cacagggcca atgaagcagt gacggacatt cacatcacag gcatacttag tgcaagttaa 188880agaggggggg aaggtgggag ggaaggaaaa gaaggtcacc aagtgacaaa gcactcatcc 188940agccaggatt tagccggtat atcccaaact ccatccagag gcctaatacc tccaaatgcc 189000tgggaatcta aatgttgttc ctctgatcat caggtaacaa cttaccctgg gactcatcag 189060gtgcactgct gagcgtgtat cgttctgttc tgtacagaca ctgtaatcta attatcacct 189120gtcccaggtc aggaagtatt tgtcttttgc caacccaggc aactgacctc cacattgttg 189180ggccttgtta acactactct tgaaaaattt aaaatctcaa gttaatatct ggaaatgaga 189240aaacctattc agaaaccaga ctgctggcac acagaagcaa ccacaacact gcctgacatc 189300aaccacctga gcgctttgct gccgtggcca ccatctctgc acttgcccag gttttgaccc 189360aggtgctctt aacatttctc acatagacat ttcaacaaat aacctttctc caggaaaaag 189420cagcatgatt tcctgaatta aattccagtt atgagaatca gaaaaacaaa atttttaggt 189480acaaaagcga agtcttccac ctgcataggc tctttattgg gagacctgaa caaaagcaaa 189540atgttttcta tgaaaccaaa caaaagaatt atgcctaaga gccgctttaa attagaagag 189600ttcttttgca gtgattatgg gtaaaaatgc agaaaacaat caaggcaaat aaagtttttc 189660ttttaactag caatggatga gaatataaca ttgatgaaca ctgctctcct tccaaacaaa 189720caaaatcaag tcaggttagc aacattctat ataccaagcc taagccttaa caatgtatga 189780tgaaggcttt acatgtcaaa tttatacata tggaataatt taccatttag tcctatcctc 189840tgtgctcagg agtctacagt taaattataa aagccctaaa tcagtctaag tgaacaaaaa 189900aattcctatg cctaatttaa aaaatactaa ggaaaactga gatggttcaa gtatctgaat 189960ttgtactgaa ataaacaaat agtcaaacct gcgggcagct gtcagttgca aaaaacactg 190020tacaaatcct gtacaattgt atcttttcac atgactaaac atccttatgg caaaataatg 190080cattactcca agatcctgaa gccctgattt aggggcagag gcattacaat acacgtctga 190140gctcattgcc tctttaaagc atttttgtca ccagaggact tccattttcc acacggcata 190200atcactcctc tgccctgctt gtcagaggca gactgcagac accacacgca tgctgacgct 190260gggaaagaac ccatcccccg atggctgagc acaaacactc cgcagccacc atctcatcgg 190320tgatttctat tgtaaccaaa gcgacacagc cagagagcaa gacacaaggg acagcagctg 190380gcgcctcggc ccaatgcaga gctgatgacg aaaatggcac ttactgttga tgagaaaaaa 190440tcattcctta tgtttaagcg aaagctccaa gttcccggct gcacctccac ctcccagggc 190500cgaggcagcc cctccccctg cccagcgtgc cctcttcact ggttcagcat cttgcacaga 190560ggcggacgca gccctctcca aggctccttg tgagcacggc tggctggctc gcaggcacag 190620ggcaatgcca gcgggaagga agacaccttc tcccctgcta actgcaattt ttaatctcag 190680ttttcttcct tccagtaaac aaatatctcc agctttttcc tcacacacac agtctttgtt 190740ctgacgctac aggattgcaa cgctggggga agaaagtcac actgttatct tggctatggg 190800ttatttatga agtgctataa acagagagaa gaaacattta gatttggggc atgcaaaaga 190860ggatttctgt cctcctcaaa ttaaatttct ctgtttcagg tttaaaagaa acagcaaaat 190920ctgaagcccc tgagtccaat aggaatgcct tcctcttgtc ctcttaaaga agaaatatcc 190980ctgtgggggt tttctgctct gcccttttgg tcctgaacat cctggtttag gagggtcatt 191040tagtttatct gctttgtgca aaaaaattct attttgcagt ataaagggaa tttattgcag 191100aggaagaaat tctgtaattt tccttgctag ctcctcccag ggtttaccat aatgtgtctg 191160aaaattcctc cttatttata gcctcagttt cttcggacaa tcctgagtgg cagtgcttgg 191220tttgtgtcta gtctacctta gactcattcc cagccttatt ctgagacttg atatcatttt 191280tcaggagtcc ctaaagacca acatttagtc attattatat ccccaccttg aaacgtagca 191340aacacgtgct ataccagtgg aatcagaagt gcagatctgg caatagttct cccaaaatgc 191400tgcatctaac tcaggcccac gtggttggct gttcctgcaa cctgctgaac gtgaacctct 191460gaggctcagt acactggacc tgcctgatgc tggccatcag gccgcttgct cattatcctc 191520tttcctttgg ggagctcccc aaccctcagg aaggcatttc tgcagggtct gggaacagcg 191580gccacccatt ctagaggcag aaggatcagg ctgatgggaa ggaaaatgga agtgaggagc 191640ctcacatctg gctctccctg tgctaaaata atccctaaat ccttccagtc ccactggatc 191700aggcaacctg ctaagaccaa cgcaagtcaa cttcgtgact agcagcattg tttggtgttc 191760cagaaaacct tgagaaaata cagataaaag gagagcaatg aaaagcttta tcagtttaaa 191820aaggcacacg tattcctgcc cattctgcaa ggcatggaaa acaatcttaa cacacaactg 191880ccacggtcgt aacttctgag tttaacggta acattttaat tgccactact ataaagtagg 191940tgagcttttc aggttggtag gcaaaattta gaagttatgc ttggtttaaa aagttccttt 192000tatggatttg atatttctga tagtgagagc aattcagtgt aaacaggaag gagctaatct 192060tcaaggtcat acggtactag gaaagtccct ccttgtcagt ggcttaataa ggacgacagg 192120ccttgctagt ggtgctacca tcagagagaa attcaggaga gaacataaaa ttgatgacat 192180ttgttcatga catgttataa catgacatct tccataatga acatttagaa agtacagata 192240agcaaaaaga aaattacaat cattttcatt aaaatgattc caaccaagag aaaaaccaat 192300gtaaactttc tgggagggta tgcttttcca cttcgctgtc caacctgtat cagatactga 192360acgctttcat cagattattc acacatgctg ctagaaagag tctttaaaga gttcctcacc 192420ctgatcccaa gtctctaaaa gaattcatca agatgtgcaa cctgccagag ggactcactg 192480aagacacagc cccacctgtg tctacagagc tctggttcat aggacaagca gagttctctc 192540attaaggaaa tggggaggga gttctgggaa acaaaagtag atgaattttt aaggagttca 192600actactgggt ttttaaaaat aaggggaggc ataggtgaga tcaggtggca ggtcaactcc 192660ttctctggca tcagtcctat tgaggggatg agatccaagg accgtctttt cagagctttg 192720gcccgtgctg gctgtgaacg ttaaccttgc atctttctgt ctatcaccca acaatttgta 192780tctcaactgt tcttaggtaa tccagattcc tccaaaacag aggaacacac atattggcag 192840ttctgaagga caccttctgt aatttagttg tctgtcttgc attcagaagt tttgctgagt 192900cacttttcaa agacgttatc aaaggctggg tgcaggtggc tcatgcctgt aattccagca 192960ctttgggagg cagaggtggg aggataactt gagcccagga gctcaagacc agcttgggca 193020atatggtgaa accccatctc tacaaaaaac aaacaacaaa agcaaacaac tatccgggca 193080tggtggtgtg tgcttgtagt cccagctacc tgggaggctg aggtaggaga attgtttgag 193140cctgaaaggt agaggctgca gtgagctgtg actgtgccac tgtattccag cctgggccat 193200acagccagac cttgtctcaa aaaaaaaaag aagaaaagag gagggagagg ggctaataga 193260cacagataaa aaaagtttaa ctcttccttt aatttgacaa atgaacgtag tagcatataa 193320tatttcttgt catcaaatca acttgggttc tacatagtta caatcaccaa acatatatat 193380agtgctttaa atgacctgtg ttgatgaaat tacctgcatg tttcataaga gtgtagatgt 193440caccactgta agaagctgcc tgagggccca tgaggacttt taaaagctcc cagagcaaca 193500aggagggaca gccaatcaca ggcctcttta tacccacacc agggcccaag taatcttatg 193560tgcacattcc tgtcccgggc agaattccca ttgtccttgg ctaaattatt taccttctct 193620gagctgcagt ttccctctcc gtaaaatgaa gaatcacact gactttgcag tgttgtttta 193680agaattcaaa tgaaataatc tatgtgagga gcctggcact taaaaaataa gcaatgttgg 193740tttcctctcc tatctatcca acaatggagg ctaccacctg aaatgatcag aaatctaatg 193800tggtttttaa ggaagcctat cctaaatccc attttttcct ccagtgaatt aaaactgatt 193860tattgaggcc ttggacttct gaaaaccatt cttaaccttg aagttagttt tatagttaag 193920atcttgcagt aacaaggccg ggtacctatg taggatcacc ttccaatctt tttttttttt 193980gagacggagt tcgctctgtc acccaggttg gagagcagtg gtgtgatcaa ggctcattgc 194040aacctccacc tcccaggttc aagtcattct cctgcctcag cctcctgagt agctgggact 194100acaggtgcgc accaccacgc ccaggtagtt tttgtatttt tggtagagat ggggtttcgc 194160catgtaagcc aggctggtct caaactcccg actgcaggtg atccactggc ttcagcctcc 194220caaagtgctg ggattacagg catgagccgc tatgcctggc cgatcttatt tttaacaaga 194280gctgctgttc cctgcccaca gggccaaagc agaatactgt gggaaggatg gaagaggcta 194340catggtgtcc tggatcctgt gggcaaatga agccatcagg gcatcccgca gagcaggttc 194400ttgtcgccac tgttcaaatg taaagtttgc agcctgtgct tccaaccctt cccttccttt 194460gtacaaggcc cagggtgaag tcagcatcag ggattctatg taaggctgat tctatgtaag 194520gctgattatg acaggtccaa gtttgctgcc tcaacccaca gattcacctg aacaataaac 194580tcagctaaga gcttcttaga ttctagctgt ctgtctcagc ctggcccttg ctttaaaata 194640agaaaaatga ttccctccca aattccttag tgctgaaggg attagcactc agcacgtact 194700gctcatttca gtgtttcaaa tgagagggtc tgggctgtca gggaagtgac actgcctaag 194760ttgaaatcct ggttacgtca catgctggct atgtaactgt gggtaagctg cttagcttca 194820gtcctcagtt tccttcttgg tatgtaaaat gggacaatgg tatctacttt acagaatcac 194880agtgaagttt aagtgaaatc acacataaaa ggcatattgt taagaatata ggcagctggc 194940aatatatttg ctattatttc aactcctttt cccccagctg ttctttccaa aaagatcaat 195000aaaattttac ccactgtttt atctctttcc caccaaaagg ttggctgaag acaggcaaag 195060agtgagcgag gaaagcaaat agcctgttaa actccaaagt gatacaagca gagcatgaac 195120gattaaaaat ggactgtcat tctaaccttc aggaaacatt agctagatct tgatcctgta 195180tgatccatga atgggtttga tgccatcata ccctcgacta gtgccaaggg cccgagaaga 195240cttaggctat aaaggcgacc acaaagacac aatttatccc acagaaatta tcttattctt 195300aacgaatgca ctgaaacatt tactcatttc aacaaacatc tgacgcgcta ttctaagctc 195360tgagaattag gtagtccagt tgcaggcctc actagggaaa gacctctcat cactagacat 195420aaccaacaga atcatcttcc actcaataaa cacacgacct aagagtcttc tcatggaaaa 195480gtaatgaaaa caaccactcc ataaatgctg gctcatatgt gctggcccca taccaaaggc 195540atgacacata tcctcacagc agacagcacc agcggcctct gatgtaagcc aattagaccc 195600attttacaga tttagcaact gaggcctaga aaggttaaat cacatactca agtttccaca 195660gtgaataagt gggaaagtca agaatggttc aaaacttgtg ctcttaatcc ttacttaggt 195720ttccatcatg catttctgta ggtttgtttt ttttccccca actggctgtg gagagaagtt 195780gaatttgggc cagtctatgt tgccagccaa atccccctaa tatttgtctt cagcaaaccc 195840tccactgcta aggaaccagg catgactcag atattttttc agaaggccaa gtatttagca 195900gagatttaaa gtcttctaag atttagcccc agcccatctt tctagctcat ctcctatgct 195960tggggccagt ggggcttctc atcacttcct gaacggcttc gatgctgctc tattctgtac 196020ctgtttgctc tcaatgtatg tgtctctctc tctgccacca gtaaccgcaa tccctcattc 196080aggaccatct gggaacgcag cccctctgta aggctcttcc taatcccagg aagatgggat 196140ctgagctctc aaaccacttg gttttacaga tgctcagata cctactccca ctaatgtaac 196200tgactcaact tacttcctgc ctgtaattct catagcattt agctcagtgc ctctgttgat 196260tgactcaggt tcacttgtgg tttccagtaa acaatgaggt aaagaagatg ccaaacttaa 196320taatttaaat tattaaattt aaataataat ttaaataata aatttaattt aaataataaa 196380tttaaataat aagataaaag tatcttactt ttatcacctc ccaattcttt attcacctta 196440taggttctcc tgtatattcc aaattctatg tgttgttcaa aacatctgag cacactgcta 196500tggaaaggac ccgtgtggcc agtggaagca cagctgcaca agtggtccca aggccaagtg 196560acggttcgct gtatgccacg tggccactga aaaaacatcc acctggagtt tgtctttaga 196620gagggaattt gaagacattt aaaaattttg ggcatcatca tcattttggg aggagagata 196680aaattatcag cgctatttct gataagattg cactggccaa tatagctttg atttgtggtc 196740ctttgattta ggactgggct gcgcccaaat ccacatccta ggacaagaca cccatgggag 196800acctggcact ctgagcccac acactttaag agtagactcc tgcccctgcc ttgagtgcaa 196860gtagtggggc agtcatgggc ccccagcatc cagaagccag gcctgtgccc ttacctgatc 196920tgccatactg aggcaggagc aacgggcatg gctccactgt ggggacacac aaatgctaac 196980acagaggcag aatttctaaa caataccccc tcccaaggat ccctttccaa atcagtttcc 197040aactcttcat taaaactact ctaattcatg taaagttgta agctaaagaa aaaatattaa 197100ggtgggatat actagcatat aattcttttt gtaacactga aagaatttcc actcattttt 197160atcaaacttt gccttaatct gtaaacttat aaaattgctg gatcatctta gaggaagact 197220gggttcagtt tactagatct gtaaagtaat tactgttcct ggagagtaat catttttagc 197280ccatttctta ccttgatccg atgacatcaa aaagatgttg ccaacgatcg ttaattttgt 197340tgagcttgtc atcgatctca gctgctcttg atttgttgct ggccttgatc aactggtcac 197400ccatctgctt taactgtaac ttgttttcac taaacaagtt tatttcttcc atgcagtcct 197460ggcaaaggca ccaaaacata aagcctggct aattagccca aactcatttc ttctccagcc 197520acagacatag agttacctct tgaaattaaa aaaaaaaaaa aaaattggcc aggcatagtg 197580gctcatacca ataatcccag cagtttggga agccgaggca agaggatcac tctagtccag 197640gagttcaaga ctagcctgtg caacacagca agaccccact tctaaaaaca ctagcaaggt 197700atgctggcac atgtctgtag tcccagctac tcaggtggct gaggtgggag gaccccttga 197760gccaagaagg ttgaggctgc agtgagctat gatctcgcca ctgcactcca gcacagtgag 197820acactgtctc aaaacctcat accttctaat gaagctaact tttcagtgag cagctaaaaa 197880gtcaggcaac gcgcatcagg caaaagcaaa atatgtaagg caataattat ttttaatagt 197940tatttaattc aaaagccctc tgtttcctct cacagatttc ttctaaccta cgtgggagct 198000gaggacaact gagcataact aattaggctg ctatcagtta cagttttcca gaggtttgac 198060tgggtggtgt tttaggtgac tacagcaaga atgttattac ttttccctgt ggagaccact 198120gatcatttca aaattatgag cctcactgct gttggcctct cccctttaac aaggggagct 198180tctttttttt ttttgagatg gaatttttgc tctgttgccc aggctggtgt gcaatggcac 198240catctcggct cactgcaacc tccacctccc aggttcaagc aatcctcctg cctcagcctc 198300ccaagtagct gggattacag gtgcccacca ccacaccagg ctaatttttg tatttttagt 198360agagacgagg tttcaccatg tcagccaggc tggtcttgaa ctcttgacct caggtgatcc 198420acccgccttg gcctcccaaa gtgctaggat tacagcaatg agccaccacg ccaggccaag 198480gggagcttct taataacaac tagagaaacc ctaactcact tggggtatca gctgacatgt 198540aaggatatgt aagccccttc cctggtgaaa ctttctaatg gagagaaagc aactctcctc 198600ggtaccatgc ccactgacaa tggtctcaca ctcccatctc tctggaacct cctctcttcc 198660aactgttctc tcaaaaggat cgaaaatagt gaagtccctt caaatttcta aaatgctact 198720ggaaacaccc gtaacattgc ccctgtttgg gcttaggagt gaggagggcc gacaattcca 198780tggtagtcat aactacatcc acctgggtgt cctcccttac cttctgtaac ttttcaatca 198840tttcttcaat actaatgtcc gctgtctgta gaactttgtt ttccatctgc accagccagg 198900cacacaactc tttatttttt tcattgaata caacccatgt attgagtctg tcttcaatct 198960cctgtttacg tatggccacc tgcaaatgaa aatacatttg gcagctgagt atagccagga 199020aggaaagcac ccccttctct ccctcaaaaa aactgagttt cattcatttt aatcagtagc 199080tctccgcact caaccttgtc tggttttgcc tcttctaaac ctagggttta gcatcaacag 199140aagttcctta cacctgtgat aaatgtcata aaaaataaca aaataaatac tgtacctgaa 199200atgtagctga ttggaaggat tgtttttatc caatactcgt gtgttactat ctggttatag 199260taaaaattag aaacaggaaa ggctagatga agatataacc caaatccttg cccttataac 199320aacgaatcaa atccttatac caacataagg acaatgtcct atttaactta aaaactatta 199380atccagatag aatgtcaatc aaacatttat aatgcaaatg gaccaggaaa actacagccg 199440tttaaatgtc cttagtgtta tgcttcaaaa caaagcaaaa ccaaaaacaa tgtacatcac 199500tcatttaacc cacttgcccg ttatgcactg gattttggat ggaaaccata aaagtatcat 199560caacagctgg tctgcagcaa gcaggctgac cccaaaatac ccaatacccc attatgcata 199620agtaacccct aaactgacat cagctaaggg tcctcctcct taaaggttta aatttcacta 199680aatataaatt taaataatgc aaatcagcct aactagatgt acaaaagtat tcccaggttt 199740taagaatctt cagaatctta gcgttaaaag gggtggctaa gagttctttt ggtaaagttt 199800tgacacccca ctatatggac caggaaacca agggtgaatt ctctgagatt ttcctgctgt 199860gtataaagaa acctctgctc ttttcggagg agaaaatact gaatgcttat atatcctagc 199920cagccaaacg tgttaaaaag gcttgaaaaa ctatcctaga aaaacaagtg ttgtgtttcc 199980attcctcggg gcatctgttt atcttttaaa aatcctggca ttttgcacat tgtcattctg 200040attgatttca taatagtaac aattaggaac atctgggccc cagtcttcct gtctacctta 200100actttcagaa cacttttcat atattctaaa cattttcttt tgtataaaaa ttgaaaagtt 200160ctaaaagaac atttaaaatt agggtacatt gtgaatatta aatactaaaa tcaaggagaa 200220cgtgtctgaa tttcaacttt ttaaatacat ctcttaattc acaaaaatga gattaaggaa 200280tttattttcg ttggaattct ctgttcttaa aacaaagatc tttaagacta tttgaacccc 200340ttaattcaat actgatcctg tcacaagcct ataccgtgta atactctatt aatacaatcc 200400cattcttaat aagttgaagt gatccattga gacaacatag cagttgatca tttctcttac 200460aggattttag aaaccagcta cttgaaataa acatcaggtt cctgtgaatg cagagtactc 200520atatgaaatg ctcccatgcc tggtgggcat gaagtaagac tcctgctgtt aggcccagaa 200580gagactcccc agtaaaggac ctcttcactg ggtccagctt ctgatacact gattaggact 200640tgccacctac acaagccaga gagcagtcta cacatctgat cagaattgca atttctgtct 200700ccacttaaag tgacttttta aaaaagctca ttaaaagcgt gcaaagttct agcatttagt 200760gctggagaaa caaaagggtc aaatctgtta aacctgttgc tgccttctaa aggagcagcg 200820gccgggccag gggtcccact gaaaagggcc tccggttcaa tgaaattaag ctcctttcta 200880ctttgtctgg ccttcagaga catttcaatc cactcccaaa cagccgtgcc ctgcagtgag 200940ctgacttacc cttaagcaga ggtcctccca ttgtctgtgc aaatgctcta tttgctcctt 201000caaaaccatc acatcttcca cgagaacgtg ccgggttaag tccgccttca tagtttgaag 201060ttctttcaag ttctgagtcc agctagccaa agactgttct agttcctgca tttaatcaca 201120agaagggggt aaaaagcctt caactcaagc ggctgttgtt tccaaacctg taggctaatc 201180ctttgattag actctggggg aggggaggcc aggagagagg gagggacctt ctgaatggga 201240aacagcccaa gacgagcgcc tgcagttgtg gttggccagt tctccaccac cttcttatca 201300aaggaaaact ctgttctacc tttattttca tttcctcagg tagctctgat tttgatagaa 201360tttattctat tttaaagtca cataggtctt catttagagg aaagtgtatt tggagtctaa 201420taatctttac acacacacac acatgcacac atacaatttt taatcctgaa actacatttc 201480agataaagtg tgattttcca tttaaaaaaa ccctaagaaa tgcattgctg ctattgtggc 201540aatcacgtaa cagcaacaac ttctgttcac agggaattat ttttggttaa cttttgaaaa 201600taatgagtaa gtataacgga agaaaaaata aacttttcta attggcaaag attagttagc 201660agatattttt ctggccatgg aaacatctat cttttaaaat gcagcattgg ctgggctcag 201720tggctcacac ctgtaatccc agcactttgg aagcccaagg tggacggatc acctgaggtc 201780aggagttcaa gaccagcctg gccaacatga tgaaacccca tctctactaa aaatacaaaa 201840attagctggg tgcggtggcg cgcgcctgta atcctagcta cttgggaggc tgaggcagga 201900gaattgcttg aacccaggag gcggaggttg cagtgagaca agatcgcacc actgcattct 201960agcctggctg acagagtgag actccacctc aaaaaaaaaa aaaaaaaaag aaaaagaaaa 202020agaaaaagaa aaaaaaaaga agcatctgtc aaattgaaac cttctgaaat ctaggattta 202080ctcaagagat ttactttcac agcagggaag gtaaaaggaa acaaccagaa cactcaagag 202140tctaccagat ttttattgtg cttacaattc ttcatgactt ggcacatttt aagggtcttc 202200actcctatct gtcaaatgaa atctgaaata agatactatg cctttttaat ttttattttt 202260tttttaagag acagggtctc accctgtttc ccatgctaga ctacagtggc atgatcatgg 202320cttactgcag cctcgactga cctcttgggc tcaagtgatc ctcatgcttc agcctccaga 202380gtagctgaga ctataggcac gcaccaccac aactggctaa tttttatatc tttttgtaga 202440gatggaagtc tcaccatttt gcccaggctg gtctcaaact ccccagctca agtgatcccc 202500ttgcctcagc cccctgaagt gtggggatga caagggcgag ccaccatgcc ctgctgatac 202560tatgcctaaa atcagggata agtgtgaaag gccctaacaa tatgttaatt ctgattgatt 202620ttcagagttc taaaagctac actggcatga tttcataact taccaacaag aatttatgtt 202680taataaaatg gcccagagaa ttctttttaa aggttgcatg aagattgagg aattaacacc 202740tttctcagaa gataaatcat taggtattta acatttttat tacctacttt tggtctcacg 202800attggttcca gtagatgaca gcagattcca cagtggaaca ctttggaaag ctacataaca 202860taaagggggc cacctgccag ctgaggtgta ccacttactg acttgaaatt ggagatctgg 202920aaacctctga gcttcaaaca gagtttccca gtcaggtggc aggaagctca gcctgtccag 202980tgccctgctc agaggtgctg gctcggccac cgagttatgc ccggccaggt ttcaatcagt 203040ttggtttgaa tacttacaat attgacaatc ggaaagtgta cagtcactgg ataaggttga 203100gactcctgtt ttactctcac tgccctgccc ttttcccctc taacactcac catgttcata 203160cacatacaca cacacacaca cacacacaca cacacacacc cctatgcata cagtgtggct 203220ctctggcaga ctggaccatt agctccacac atggcaggga ccatgacaac actgctcacg 203280gattcagtgc ccagaacctg ccatggtgct gctgcacagc aagttttcag ataccaaaat 203340gtacggctga aaggaaagca atttggctgt atgtctcgag agctttaaaa tgttcataaa 203400gactaaatga aaaaagcagg gagtcaaaca atatatagaa tgaggtctct tgtggaagtt 203460tactagagct gacgatcagt ttgtataaaa atgtcaacaa tcatgcctgt ctttaaatta 203520aattgctccc tctccctctc cccctcccct ccccctcccc accccctccc tctcccttct 203580ttccacgtct ccctctgatg ccgagccccc tctcccttct ttccacggtc tccctctgat 203640gctgagccaa agctggactg tactgctgcc atctggcctc actgcaacct ccctgcctga 203700ttctcctgcc tcagcctgcc gagtgcctgg gattgcaggc gcacgccacc acgtctgtct 203760ggttttcgta tttttttggt ggagacggag tttcgctgtg ttggccaggc tggtctccag 203820ctcctaacca cgagtgatct gccagcctcg gcctcccgag gtgccgggat tgcagacgga 203880gtctcgttca ctcagtgctc aatgttgccc aggctggagt gcagtggcgt gatctcggct 203940cgctacaacc tccacctccc agccgcctgc cttggcctcc caaagtgccg agactgcagc 204000ctctgcccgg ccgccaccct gtctgggaag tgaggagcgt ctctgcctgg ccgcccatcg 204060tctgggatgt gaggagcccc tctgcctggc tgcccagtct gggaagtgag gagcgcctct 204120tcctggctgc catcccgtct aggaagtgag gagcatctct gcccggccgc ccatcgtctg 204180agatgtgggg agcgcctctg ccctgccgcc ccgtctggga tgggaggagc gcctctgccc 204240ggccgccacc ctgtctggga ggtgagggga gtctctgccc ggccgccccg tctgagaagt 204300gaggagcccc tccgcccggc agctgccacg tccgggaagt gaggagcgtc tctgcccggc 204360agtcgccccg tccgagaagt gaggagcccc tccgcccggc agccgccccg tccgagaagt 204420gaggagcccc tccgcccggc agccgccccg tccgggaagt gaggagcgtc tccgcccagc 204480agccgccccg tccgggaggg aggcgggggc agcccccgcc tggccagtcg ccccgtccgg 204540gagggaggtg gggggggcct ctgcccggcc gccccttctg ggaagtgagg agcccctctg 204600cccagccgtc accccgtcta ggaggtgtac ccaacagctc attaagaacg ggccatgatg 204660actatggcgg ttttgtcaaa tagaaaaggg ggaaatgtgg ggaaaagata gagaaatcaa 204720attgttgctg tgtctgtgta gaaagaagta gacataggag actccatttt gttctgtact 204780aagaaaaatt ctgccttggg atgctgttaa tctatgacct tacccccaac cccatgctct 204840ctgaaacatg tgctgtgtcc actcaggatt aaatggatta agggcggtgc aagatgtgct 204900ttgttaaaca gatgcttgaa ggcagcatgc tcgttaagag tcatcaccac tccctaatct 204960caagtaccca gggacacaaa cactgaggaa ggccgcaggg tcctctgcct aggaaaacca 205020gagacctttg ttcacttgtt tatctgctga ccttccctcc actattgtcc tatgaccgtg 205080ccaaatcccc ctctgcgaga aacacccaag aatgatcaat ataaataaat aaacaaataa 205140ataaaaataa aaataaaaaa taaataaata aattaaattg ccccagactt gcaaccaaac 205200tgtcaaagag ataagtttcc accatcttca ttcaaccaga taaattatct ataccacaag 205260aatacctgaa aatttccaat tttttacgta acatgggatg taaaatttca agttgtttgg 205320ctgcgaaaag cggtgtgttg cacaggattt gactggactc tgcagtatca ggacagcatc 205380taagccttgc ctgtggactc aattaccagt ggaactgtca agtcccattt ctctgtacta 205440ccatttgggt aaaggaggac caaaaaagaa cgggtatata aataaaattt gacctgcata 205500tgcatactct atctttggaa gaaaatacaa caaacttgta acattgattg cttctgtgga 205560gaggaactga taagagaaaa attttactta tacaaaaatt atttaaaaac caaactgttc 205620caaggactca atctttggac aagtgttatg aaacacagat gtccattgta gcaattttca 205680taatagtgaa gttagaatat atgtaaaaca tatatgtaag gaaattattc cattcctcaa 205740cgtggctgta gtgggaatat aatttttctt ttttatcttt tatctttttc tttttgcagc 205800tgtgcttaga agggaatata attttatgag gaggaaagtc aagctcaaat tggtcaagtg 205860gctagcgtat ttcccccagg tgaatcaggg atgaaacaca ctgacctgga caagctttgg 205920gttacttggg atcttttcag aatatggtca cgggttgaac gaactcaatg ctttttgcct 205980ttccctgatt actttccact gataaatagg atgctgagac cgtgaaagct gaaaagatcc 206040cactggaaac tgagaagtgt gatacttgag aaaaattatg gctgctcaag ggtttacatt 206100tcatgttctg cacaggtttc tagaccttat ggaaggaaac tctgtgtttc agctgagagc 206160atgactccaa attatttccg ttgttaccaa agtcttgctc taagcctaat gaggtaggga 206220tgataaaaac tttgaaaatt tggcatagaa atcttgttaa cctaataaag ccactgactg 206280acacatcacc accaaatatc acatttaaca gtgaaatgtt agaatcattt tcttctaaaa 206340tcaacaatgg aagactcagc tattaatact tctgcttaac aatgttctga aggtattgac 206400ccgtgtagga aaaataggaa aaaaaaaagt atattaaaaa gaagaaacaa acaaaaataa 206460ccttgtcatt attgacagat gatgatggtc tcatagacac ccaaaaaaat acataaacga 206520attattggaa ttaacataag agtttaacaa gatggttaaa cataaatatg caaagaccaa 206580ttacatttct aggcatcagc aataatcagt taggaaatat aattagaaaa aggcagtatt 206640ttcaatagct tcaaaaatat tgcaccaaga aataaatcta acaaaagata tatcttttgt 206700gattaggaaa attataacaa gaataagcca ggtgtggtgg ctcaggcctg taatcccagc 206760actttgggag gccaaagcag gtggatcacc tgaggttggg agttagagac cagcctggcc 206820aacatggtga aaccccgtct ctactaaaaa tacaaaaatt agctgggcgt ggtggcgggt 206880gcctataatc ccagctactt gggaggctga ggcaggggaa tcccttgaac ccggaggcag 206940aggtttcagt gagccaagat cgtgccactg cacgccagcc tgggtgacaa gaacgaagct 207000ccatctcaaa aaaaaaaaaa aaagttataa accattagtg aaaacaacta aataatatga 207060ccaaagggaa atatatacca cagtcatgag catgagcagg aatactaaaa ataattatcc 207120aagttaatct aaaattcaat gcaattcaat taaaactctt atcagaactt ttcatggttt 207180aacaagctgt tcctcaaagt tacggaagat caaagaccca gggacatcca aagtaatgtg 207240agagaacaag gtgtgggtat acatcacacc tgaaataaag atgtgttaca ataaagtgca 207300gtaatcaaga cagtttgata ttaaagctga catacacaga ccagtggaac agagaactga 207360gaaacaaatc ctgcgtttat ggaaatttgg tatttgacat tagacaataa ataggcacca 207420agacaactgg ctatccatac gaataaaatt agaaaatgga tctctgcctt aaaacgtatg 207480caaaaatcaa ttcctcaatg aataaagatg tgaatgtgaa aatcaaaaac taaaaacttt 207540ttgaagagaa tgtaagcacg tattttatga ctttagtatg aagattttgc aaaggaagcg 207600gaaaatacaa atcatatagg aaaatactga ccaattcatc cacattaaca aaatatcttt 207660tgttcagcca gcaataccaa ggaaaacgca gaaaaacagg ctctcagatg ggaaaagaca 207720tttgcaatgt atatactcag tagaagagga atatttagaa taaataagca gctccttcaa 207780ctcaataaga aaaagacaaa tagcccaatg gaaaaaaatc agtaaaaggc acaaataggc 207840aattcacaaa agggcaaata tgaaaaaaat gtgcaactga ctaataaact ataaagagat 207900actatctcac agctgctgga ttagcaaaag tttaaaaaaa aatctgacaa taccaagtga 207960ctacagaagg gatatataaa ttgttgtata ttcttataat gcatactata cagcagtgag 208020aatgaatgtg cattaggtgc caacatctat gactccaaat cacaatgagg gaaaaaagtt 208080acattgtagg ccaggcgctg tggctcacgc ctgtaatccc agcactttgg gaggccgagg 208140caggtggatc gcttgaggcc ccaggagttc aagaccagcc tggccaacgt ggcgaaacac 208200catctctact aaaaatacaa aaaaattagc caggcctgat ggcacatgcc tgtaatccca 208260gctacttggg aggctgaggc acgagaattg ctggaacccg aatggtggag gttacagtga 208320gccaagattg cgccactgta ttccagcctg ggtgacagaa ggagactctg tcttaaaaaa 208380agaaaaaaaa attacattat atatagtact gcttatataa agttttaaaa tttatgtatt 208440ttgtttactt tttagagaca gtcttactct gtcatctagg gtggagcata gtggtgcatt 208500catgagtcac tgtatcctcg acctactggg ctcaaaggat cctcccacct cagcctcccc 208560agtggctagg atcacagtca agtgccacca catccagcta attaaaaaaa attttttgct 208620gtacatatgg ggatctcact ttgttgctca ggctactttc aaactgctgg actcaaggga 208680tcctcccgct gcagcctccc aaagtgctgg gattatagtt gtgagccacc atgcctagcc 208740atataacgtt taaaaacaga aagcaattct acatctcatt gacagcaggc tgggcgcctg 208800ctgtcacttt gggaggccaa ggcaggagaa ttgcttgagc ccaggagttg gagaccagtc 208860tgggcaacat ggcaaaaccc tgtctctaca aaaaatagaa aaattagccg ggcatggcgg 208920tgcatgcctg tactctgggc tactcggggg ggctgaggta ggaggatcgc ttgagcttgg 208980aagttggagg ttgtagtgag ctgagactgt gccactgcac tctagcctgg aagacagagc 209040cagaccctgt ctcttaaaaa caaacgaaca aacaacactg acacctacaa acacatgtat 209100taaaattatt aggacatgtg taagaatgat gaacaccaaa tccagggtta ctgacacctc 209160taaggagaag ttagatcata caggacactt gaactgtgtg tatacttttt aattttttag 209220ctaggtgatg gtgtcatggc agtttgtcat attatttgtt atgcctttta gcacatcaga 209280catttttaca acaaagaaaa aagatgtagg gagaaagaca ctaatttctg gcctaatttg 209340aagtataaca ctattcattt cttcctaatt ttttcaattt gatccccgaa agtcactgaa 209400ggaagaaaaa aattaaaata ccactccaac agtctctcaa tgtcttttaa acatcaaagc 209460tgcagacaga aaagagatgc agggagttgc tgacctggga attttcctgg ccccaattcc 209520tatccataag tttgccttgt ctccagcaaa ggcctaaaat atccaggaag ttttgctagt 209580gtggtattca ttaggaagaa tatatagttt ggatggtttt ataaaaatgc tatactggag 209640caaactccaa cctactggct ttcaaaaatg aattctttag tcatatttta ctcttgtttc 209700aaagaagagg acatttgata agggaacagc ttttacagga atctcagagg ccacatctta 209760tattacagag gaggagcaga cagaaagaag acctgaagga gaaaacccct aagatcctaa 209820gaagtgagtt tttgcaaaag tctttctcgt cttcaaacaa caatcgtaaa aagcatcaat 209880tctaattcct taagagacga gcatgttatg ttagacagat cgggaaaata ttttctgatt 209940gaaaatgaaa actcagtttc aatacagaaa gaggcaaaga agcacttcaa agtactcaga 210000aaacagctaa gtgattatct tggccacttg tttggatttc aataccttaa tcagctcttt 210060ttcgttatgg aggtcctcgt gaagctctgg aagaggatct tcactttgtg cctttaaaac 210120ttgcagcctg cttttcaact ccttgatttt cttttcacac tggtcccagg tctatttgaa 210180aacaagatta aaactggtaa gtattttgct ctttagaatg aattgtagaa atagaatagc 210240tcatttaatc cacataatct ttctattaag tttgttttta ataaatcaca cagacaccca 210300gttccctgtc ttaagcagac tatggtctcc aaaagcagaa ggaacaccaa tatgactcga 210360ccctccttgc agctttacct gtggctcatt tcaaccacca ggctaggctt tgcttgaaaa 210420tagttcttcc gggctgggca tggtggctca agcctgtaat cccagcactt tgggaggctg 210480aggcgggtgg atcacctgag gtcaggagtt tgagaccagc ctggccaaca tggcgaaact 210540ccgtctccac taaaaataca aaaattagct gggtgtggtg gtatgtgcct gtaatcccag 210600ctacttggga ggctgagaca ggagaattgc ttgaacctgg gaggtggagg ctgcagtgag 210660ccaagattgc accactgcac tccagcctgg gtgacagagc aagactccat cttaaaataa 210720ataaataaat aaataaataa ataaataaaa ataaaaaagt tcttcctctc tccccaatcc 210780ctctcatcta accaatgtga tttctacttt aattttggaa aagttcacac ctaaaaggct 210840actgtacgga gtaactgtta gcattgacag tctttggtat atgctatgac agcaccacag 210900aacacaagca ctcagccacc ttccttgtct tcttttccac cgtcacccct ctcctctgct 210960ggctgtcttc tcctttctac agcctggctt tagtgacctt ttcacattca accttcactt 211020tccacttctc accactgtgc tgactaccac cttctgctta aagcactttc ctgtttacaa 211080agcattttca actttattaa ctcattgtaa ttataataac cttaggagat agacaaggaa 211140agtattatca tatttaaaac aagtattatc atatttaaaa tgggaaaact aaggctcaga 211200gaaaaatgac tagcccgtgg atctggggtt aaaagccaag tcaagggtca accatctctt 211260ttcttcaaat tttaactgtt gaacaaaata ggtgcctgca ttttcaacta tagggtattt 211320atattcgatt cattaggata aacttctttc ctgaagttac taaaactata caaacctaca 211380atgaaacact attattcagt acagctgata cctaaacata cttgggttgg cctcagtcta 211440tggtaacctc tcactgaagg gaggccactt gtgtagcaat attaactatc tggaactcaa 211500ctcaaaagaa agcaaaggag aagcaaaacc atgagttctt caaactgtta tttgcaatta 211560tcattatgct tcacaaacat ggcagatgct taacaaatgt ttgctagtta atgaattcca 211620tatagtccat atatcaaata aaattcagat actttattca caggagaaat tttcaagccc 211680taactcagca tatatttact cttaattcag aaaaatatgt ctggtaagtt tggttatcta 211740gttttggcac acacgacaga ctagagagag tgggctataa gaagtaagtg agcttggaga 211800aagacaaagt aaaaatagac agtttgacat taagaggaga gaaagaaaaa tgataaaaac 211860catatcagaa ctcaaagagc acagtagttc agggagggcc acaggttcaa agtaattcag 211920aggccttgag aacaacttgg tatcttagca taacaaaaca ataatgattt tcctaacagt 211980gactcaaaat ataacactgt agttcttaaa ggtataatca tacattattt ggaaaattca 212040cttatatgga aaagtgcatt ctctgatgat agcagaaaaa tgaagtgttt ctgtctgttc 212100catacatgga acattagttt tatgtattga actttcattg aatatgtttc ctgtttcata 212160aaagtaagta tcttaataga atctcaagta tttaaatact aaacattgag ctcttgcaat 212220gctatatcat catttcacat taagtaactg gcatgactta cagattacag cttgagttgg 212280aaactcatga tactatagtt tcctaaaacc atttccattt tcacaccaca aatgaaaatc 212340tatgtactga atttgaatat catgaatgga aaagtaagtg gtgagtttac ctctacagtg 212400ctctggaact gcttaatcat ctctgccagc tggggctcca tgtctttcca gctgtcctga 212460agttgactga ttctcctacc cacagactct ttagttttca ggtcagttgt gagcagtaac 212520ttttctccag cttccaaggt tagggcacag gtagttcgcc tcctttgaaa atgaatttct 212580ttattctaag aaaaaaaaga gaagtaattt gcttaggttt ctgatgtgtt caaaatgtga 212640ttgtgtgtgc ctgtgtatat ttccttcaga gcttttctta agagttaaac tagatataaa 212700ttagacaaat tgggtcagaa tgtacataca cacacacatc aaaagctaat gttttacact 212760gagtagataa gcataccact aagtgaggtg ttcttaaaat aatttccaaa gattggtgag 212820ataagattct cagtgtaatg attagcagca cagagaaata aaaaaatata catcatagaa 212880taatagcctg caaggtaaat ggatctaaga gtattaagaa agtgaatgga gtaacagacc 212940tgaaatcaag taacagacct tatattacgc taaacaaatg taaaaggaaa gaacgcgaga 213000aagggtaaaa tacaaaaaat atacatttct taagcttttt atgagcacat aaacatgtac 213060attgatgtgt gccccaaagg cgcagcttta aaaaatttca tccgaatatc atgtaggctt 213120tctgaaaatt aaaaacaatt taaaaacaat agttttagac ccgaagtgat ggctcatgcc 213180tgtaatccca gcactttggg aggccaagac aggcagatca gttgagctca ggggttcgag 213240accagcctgg ccaacatgga gaaaccctga ctctactaaa aatacaaaaa ttagccaggc 213300atggtggtgg atgcctgtaa ttccagctac ttgggaggct gagtcccaag aatcacttta 213360acctgggagg cagagattgc agtgagccaa gatcacacca ctgcactcca gcctgggtga 213420cagagtgacg ctttgtctca aggaaaaaaa aaaaaaaaaa tttaagagac aaggtcttgt 213480ttatttttat ttttttctat attttagtgg cggtgttaca gctccgtgac tgctcctgca 213540gagcagggcc gctccgcagg cagtgtgcag agagtagcga aggtcctgtt ttttaagaga 213600tgcgccacca cacctggcta catgggcgtt ctttaaagag attgaaacta ggtatttata 213660atacaaaaat aaagtaaaaa ctttaaaatt gatccatact cccaaaggca tttggtttta 213720gaggaaaaat taaatagttt ttgtctgttc taaatatgtt ttcctggctg ggcattctgg 213780tgagtgcctg tggtcccagc tactcgggtg gccgaggtag cctcaccaga agtttgaggt 213840tacagtgagc tatgatcacg ccactgcact ccagcctggg agacagagtg agtacctatc 213900tctaaaaaaa taagtaaata aataaataat acataaatat tatttccttc accttgaaat 213960ttcttgaagg agacaaagtg tgggcatttt tccaaaatgg gagctgataa acatcaacag 214020agcatcacta atttacaaag gcatttctga gaagcctgaa gatcacccat acccaaagga 214080ggaaagccaa atgcaccctc aggagcagca cacaaataag agaaacagca actggaccac 214140aggcactcca cgtgcatctc tccaacatat tgtgctgcgc aaatgagcca ctcgtattag 214200acctgctcct cccttagtct tccctaactc tgtcaacagc acactattca ccaagatgct 214260tgagcttcaa cccagagtcg tccttcatct agcttttcct ttaccagact cacaaaccga 214320cactccaaaa tacggtgttt tgacacgctg aactgaagaa gtctcagggt ctctctaccc 214380taacgcactg cgtctcctac agaagctgaa gtcctttatc tgcctaagac ccggactcac 214440caaggagaat gattgttttt tctttccctc cctgttacct cattatctta ttgcagaaaa 214500gaagacccag atgtaaccac acccaaatag gctctttcaa gatgactgcc tccagcgatc 214560actgaaattc caaagataac cttttttttt ttttccagac aggtcttgct ctgttaccca 214620ggctggagtg cagtggtgtg accatggctc gttgcagcct caacctcccg ggctcaagtg 214680tgaccctcct gcctcagcct cctgagcagc tgggagtaca ggcgtgcacc accacacctg 214740gctaaatttt gaattttttt gtagagacag gggtctcact atgttgtaca caggctggtc 214800tcaaactggg ctcaagggat cctcccacct cggcctccca aagtgttggg atttcagcca 214860attctcatca aactgtggca cctctgtccc ctcctctcaa actcatgtgg acacatatga 214920tagccttcat tgttagagtg tggcagaagc gatatatgtg acttctgagg ttgggtttat 214980agggcaatac agcttgttct cgggataaga atctttgaaa ccattggggt accatataag 215040aagtctggcc actggaagcc ctcacgtggt aacacaacag agaatgatgc ccaaggagag 215100gagctccact gtcccagctc cttgctgctc cagttgttcc agcctaagca tcagtcatgt 215160gagtgagctt cagatgacac cagccccagc caccatctga ctgcaactgc ctgagagacc 215220ccaagcagga accaccaact cctgtcaacc ccaagaatga cgagacagaa tgatgagtgt 215280cattatgcag cgataggtaa gtgagatgtt aggattccta cttttccttt agctccccta 215340caatcaggaa cagccagagt gatctttaaa aaatatgggg ctgggcacag tggctcacgc 215400ctgtaatccc agcactttgg gaagctgagg tgggtggatc acctgaggtc aggagttcga 215460gaccagcctg gccaacaggg tgaaaccttg tctctactaa aaacacaaaa attagctggg 215520catggtggcg ggcgcctgta atcccagcta cttgggaggc tgagacacga gaattgcttc 215580aacctgggag gcagaggttg cagtgagcca agatcgcaac actgcactcc agcctgggcg 215640acagagtgag actccgtccc aaaaaaaaga aaaacaagca aacagtgtca tgctgccctc 215700tccctcagct gaaatgcttc aaaggctccc tggttccagc cagaccaggt aatccagctg 215760ccacacccaa cctgcaaaac actcctccct acccgccttt ctagttcacc tcctgccact 215820actgccttgc cagctctgcg ccagtcacac tggccttttt gccgttcctc tgtgctgagc 215880ttgttctcat cttggggact tggagtaagc ctctccttca gtctgaaagg ctctttcctt 215940agatcttgca tggctggctc cttctcatca ttcagtccca ggttaaatgt cacatggcca 216000gagacaaccc aatctaaagg agccacacat ccccatctct actggccctg ttttaatgat 216060ctacgtaagt cttattacaa tctgatattt tattatttat taatttatct gattaattat 216120tctggtttac tactttgtct gcccttatga gagcgaaggt cttctctcca tccacagaat 216180acacccagca cttaggacag tgattggcac agagaaggta ctcaattaat atgcgatgat 216240tgcataatga gtgagggctg tacaggattt caggagggtg gtgtttgttg agaggcttct 216300ctaaggtgga gagtagctct acagctccat gggacatgcc aaccacctat gctaatcctg 216360tgctcatcag gcaagtttct tttggaaagc tttgttggct gaatgaattt gcccctttat 216420ttttattttt tttaaatcag tttttaaaat tttgtgagtg catgaggttt ttaaatatag 216480gcatgcaatg tccaataagc acatcatgga gaacggggtt tccatcccct caagcattta 216540tcctttgagt tacaaataat ccaattacac tccttaagtt atttaaaaat atataattaa 216600attattatcg actatagtca ccctactgtg ctatcaaata gtaggtctta ttcattctat 216660tttttttgta cccattgaat ttgcccttca agagtaaaca atgccatcac tgtacattac 216720tacactaatg gtcaatttat acattagtca gtggcttaag aatctgggtg gtttgagtct 216780gttgccctaa acctccccca atttattcac ccatctccac tccatgcctc ctctcatggc 216840ccaagagaac cctagtattt ctggatgaat gggtaatatc gggtttccaa ccccacaatg 216900ctagggactg ggctgtgggg ggtggtgact gcaccttgct ggcctcacac tgcagagagc 216960ccacttggat cacatcatca tcaaaaacat taaaccaaag gtgaatgaac atcttgcaaa 217020caatgaccac cctgtggttc tcacactgct acgatgcata cactaccttc agctcatgga 217080tcagacttct ggtttggtag aggctgaagc gctcctggcc cttcactgca gatagcaggt 217140ggctggtgtc agtgaggaag cgaaacaagt tctccacaga agtagtgaaa tcttgccact 217200gcctcaccag cccatcaacg tcacccttcc tctgccgaac accctggaca gcattctgcc 217260accgatccgt cagctttgag aattctgtaa taaattctgg tctggggaaa aacaaatggt 217320tatagaatcc agacatcgac atgtagaaaa aataactatc agtgggtaat agcagctcag 217380attcagtttt tataagtaca atttacatga aaaaaatccc aacttctaaa cacctgagtg 217440ctattcaatt taaacatgat taaggctttg ggaagggcag catgcagact gagtttagca 217500gaaggtatct gcacgtaacc tggtattttg ccagcatgca taaccattcc cttaaacaaa 217560ctgaagctgt tttaatatac attaggccaa acgtggaaag gaaaactaga aactggatca 217620aagggaaaca gatgaacagg caggtacttt gtaacactat ggattacttg agtgtacttt 217680acataatttt agcaataaag agcatgcttt attgcagcta ctcaacagtt cttttctgga 217740taaataatat ccttcacacg agaaataagg cctcttgttt attatgggaa agtcatgttt 217800ttctgtacag atcaaataga aattagctta tcatcctgga actggcttgc atttactgct 217860tcagagctag ggagaaaagg gctttacatt tcttagttat gctgaaagaa gattcagagg 217920tgtatgggtg gtgatgtgga agaaaaatca tacccattag gacatttaga gaagtattta 217980aatggccagg ccatttccag gctatctgcc atgtgtacag ctgcatgagc agactcactc 218040agcattagca gactggccaa aggtgctctg ttcagcctga ttggcactgc ccagtgaccc 218100taagcccaca tatgtagcat ggagagaaag cccttctttc agacttatag gatttagcta 218160cctactggga acattaggaa taatcatttt ttagaaatta ttttttaagt cgcaaaacat 218220catgaggatg atggtagaga aggaaacagt acagaagaat catttggggg cacaaactta 218280gttaactata cttgagaagt gggcattttc caaagcttct gggctgctaa ccccatccac 218340ctaccatacg gaaaaggtgt ctgtcacaga tgtttttatg gtcacccagc aagatctagt 218400cccactacat agttagaagc ctggtcctat ggtgatctgt caaccaacag ggcacagggc 218460accaacatgg tcaaggttca catggtggaa agcctgccct gactgaatgc attaccttcc 218520atccgcccac aaccttcatg aggcccagac agctcacctg ttctctattt ctgttgtgtc 218580caggagttgt aaggactggg tgacatagga atcagcaatt gtctggttta tagaaacttc 218640agcttctaac atctgtatat gagtccaagc agaaaatatc agctagtaaa attcatttta 218700tgaatctcgg aacctacatt tcctattaaa atctaagctt acaaaggata tgagggataa 218760ttaattttca tattatttga acctactgca aaaaatgttc aactatcata tcaacatcac 218820ctacgaatat attcttaaaa gcaaacaggt ctaataataa ttacatctaa tacccatttg 218880ttgggcattt acgatttggt aggcactgta aaacatgcat tgtctcattt atttctcagc 218940cttaggattt aagcactatt attacatcca tttgaagagg aggaatctaa ggtataaaga 219000ggttcagtaa cttacttaag gccactcagc tttttggcag gggtgatttc attttaattg 219060gatgatctta atgtagcaat gtagacttca atcagttaca ttaaaaagtt gcagtgaagt 219120aattctgcac acttttgaaa tgtgtccttt gtaatcccag cactttggga ggccgaggca 219180ggcggatcac ttgaggtcag aagttcgaga ccagcctggc caacatggtg aaccccatct 219240ctactaaaaa tacaaaaatt agccaggtgc agtggcacat gcctatagtc ccagctactc 219300ggaagggtaa ggcacgagaa ttgcttgagc ctggggggtg gaagtttcag tgagccgaga 219360ttgcgccact gcactccagc ctgggtgaca gaggaagact ctgtctcccc ccaccaaaaa 219420aagcaggatc tgcctataac caccaggagt attttcaaat acatgtcagc ataagcactg 219480aggaaactcc tgtgaaatta ttaacgctac agttataatt ataccaggtg ctttaatact 219540atggagaatt attgagtgaa aatacaaaca tgagtcgaag tattttatta tactcttgat 219600agaatggtat aatgtttgca ttttgtggaa gcaaagttgt actagaagct tccatgtact 219660gcagaaaatc taggagtcct ctccctaata aaatagagca gtaaatgttg aaatttatga 219720aacgtacaag aattttacaa atgaccaatt gacaactgaa tttgcttaaa ggtatacatt 219780ctgtgtttag ggactgatag gtacactccc tgtgttttat ttaaagctat ctttccctga 219840ctcctgcccc agatgagcgg ttcaagggtg gcagagaaca caggtttacc ttataggttt 219900tctgctgctc caggagctca ggaaggctgt tagccacatc cactttgagt gcttcttcta 219960tcttctccaa aagttggatc cacttttcac agcaataaag aaacttttca ttcaatccaa 220020ttccctgaag ctcactacaa aggtttaaaa caaaacacac ccatgttaac aatgccattc 220080ccgagctgaa gccgttaatt ttaccttaat tgaaacaacg acagagaagg gatgttctaa 220140cctgcagcgc tccagtgccg tggccgtggc ccgaatccat tgccggttca tattttgtaa 220200cgtcttcaca gctacgtcac taagtgggag cttgaggctc acttcattca aatgttcaat 220260atcaggtgat tgggctgtca gtgccagcac atgattctat ttttaaaaaa ttgaagtaca 220320ggtttttgga tgccaataag atatctagat gacaaaaaac cctccttacc tcaaagataa 220380aggaaaactg gggcctgaac aggcaaatga cttgcccaca gtcaaacagc ttgctaatgt 220440tagagtggga cctgaattca gctatctcaa tgctgtgcct tctagaatac caacctgcaa 220500cacggccagc acccaaacag cccaggaaat agaaagttat ctcagatttt gtcaccgaag 220560aatacagaaa ataggctttt ttatttttta tttttcggaa tggagtctca ttctgtcgcc 220620caggctggac tgcagtatca tgatctcggc tcactgcaac ctccgcctcc tgggttcaaa 220680cgattctcct gcttcagcct cccaagtagg tggaattaca agtgcctgcc accacacctg 220740gctaattttt gtatttttag tttcaccatg ttggccagct ggtctaaaac tctgaccttg 220800gatgatccac ctgcctcggc ctcccaaagt gctgggatta caggcgtgag ccactgcacc 220860cagcctaaca tgctttttaa aaaaggacca attctgggct tttaagtccc atttagtctg 220920ccaataacct ggccttcaac aaacaggtca agcacagagt gatgaaaatt tgattcttaa 220980aataactcta gcagagcaag ggacttaaac acccatattt tgatagaaca tgacattgca 221040agaaagcaaa cttatcaaaa tttcattttt aaaaagatat tccagcgaca gcttctaagc 221100tgacaagcaa acatccttaa caatattgaa gttaccacta cagaactctg ctgtaatatt 221160ctaggtcagg ataagcaaac tttttctgta aaagggcaga tcataaatat tttaggcatt 221220tgtgggccat atggtctctg tagcaactag tcaactctgc ccttgtagcg tgaaaactat 221280aatagacaat atgtaaatga aagggcatgg ctgtgttcca tcaaaatttt atttaaaaaa 221340aaatcagtga tggacagtgc accacagttt gttgactgct gatctaagtt atgaatattg 221400attcctagga gagttacaag accagatcta attctttcag tcatttatgt ttactaaact 221460aaacttacga aggtaaagaa aaatgcataa tagaattttt tttatattaa actaaccaga 221520aaaattcaat taatcacaac agccaatttc tagattattt cagttcactg acggtaatta 221580tgaaaaaaca tctactacaa attactctga cgtattacta aaagagatcc ctatgtcatc 221640ctactttttc taggcctgag ggaaaatagg acagtaatga atggcataaa aatgactgtg 221700ctcaagggtg agttaggcca ggcgcagtgg ctcaggcctg taatcccagc actttgggag 221760gccgaggcag gcggatcacc tgacgtcaag agttcaagac cagcttggcc aatatggtaa 221820aacttcatct ctactaaaaa atacaaaaat tagccggaca tggtggcaca tgtctgtagt 221880cccagctact tgggaggctg aggcaggaga atggcttgaa cccgggaggt ggaggttgca 221940gtgagccgag atcgtgccac tgcactccag cctgggtgac agagcgagag tccgtctcaa 222000aaaaaaaaaa taagagtgag ttaggatgca agcattccat aagttcacag taatcagctt 222060atttttttta accttatggt gagtctaagc ccttagaaaa gacaaatatt ggcaatacca 222120ggaatgagtc aggattttct ttttcctgtt ggtgaagtct atccaaattc tcttcttcat 222180gcataataag accttttaaa gattccagcc ggactccaaa atccttaaac tgtgagagaa 222240caaactagaa caagagaaat tttcattaga aactattttc atgtacatat aaaacagaat 222300taaacacagg cttgggagct gctaaaaatt caagatgttt ttggctaggc acggtggctc 222360atggctgtaa tcccagcact ttgggaggcc gaggtgggaa gattgcttga gcccaggagt 222420tcaagaccag cctgagcaac ataggcagac tccatctctg caaaaaataa aaaattagct 222480gggcatagtg gtgtgagcct gtggtcccag ctttttggga ggctgaggca ggaggatcaa 222540ctgaacctgg gaggtcaagg ctgcagtaag ctgtaattat gccactgcac gccagcctgg 222600gtgacagaat aagaccctgt ctccctgtct caaacaaaca aacaaacaaa caaaaaaaaa 222660caaaaaataa aaaaaagtaa attcaagttg ttttcaacac ccagaagttc actattatct 222720caagaatcat agataaatat tcagaagccc cctgattttt cactcgtgcc tttgagcctt 222780cccaaatgga cagatcaccc gcctgaggag accacataga gagtcagtga gcactccgtt 222840ctctcagtcc cactagttaa atgtgctttt ttgtttgttt gttttcctgc tggtactgag 222900atgtgatact gcaactccat ggccttctgt gtagcttgtt gacagctcct gcactaaagg 222960ctttataaaa agtgaatgaa ggctgagagc agtggctcat gcttgtaatc ccagcacttt 223020gggaggccaa ggtgggtaga tcacttgagg ccaggagttc aagaccagcc tggccaatgt 223080ggcgaaaccc catctctact aattatacaa aaattagccg gccagacaca gtggctcacg 223140tctgtaatcc cagcattttg ggaggccaag gcaggtggat cacgaggtca ggagattgag 223200accatcctgg ctaacatggt gaaaccctgt ctctactaaa aatacaaaaa aattagccag 223260gcatggtggc aggcacctgt agtcccagct actcgggagg ctgaggcagg agaatggcat 223320aaacccagga ggcggagctt gcagtgagct gagatcacac cactgtactc cagcctgggc 223380gacagaacga gacatctcaa aataaataaa taaataaata aataaataaa taaataaata 223440agcaagccag gcgtggtggt gggtgcctgt agtcccagct atccgggagg ctgaggcagg 223500agaatcgctt gaacccggga ggtggaggtt gcagtgagcc aagattatgc cactgcactc 223560tagcctgggt gtcaggtcaa gagtctgtct caaaataaaa aaaagtgaat gaagacacga 223620agatatgaca aatcatcatg ctttaagcag ctacttctat ggctcaatta cttgactcag 223680aaatccaaat aactgtgaga tctgaacaat gtatgcaata taatacatat atgacttcta 223740gaactaacat cttcaataag gaagaaatga ttgcttaaga tctgcccatg tgattgaagc 223800tagaatcgta tacatttggg agcagcttca gtctgtaatt ttgctaagga gcagcccgta 223860aagcactact gcttcaaagg atacaaggga ttgaggatgg aatgcaaatg tctaagacta 223920aaacttgaat ggaaagaaaa gatgaacctc aaattcattt gctttcacaa gcagcatctt 223980ttccaaataa gcagccctct ggagagagtg cagggggccc gggaggagca tgtgggtgct 224040ggactctgcg ggttgtggga gtcgatccag gacactggaa ttttggagaa aggcttcttt 224100tgttttctgc acatcatgct gcagctcctg cagaagaaaa agggatggca aacacctctt 224160cctcctttca aaccacaatt ccctacccgc tgctgctcca tcaaaatcta ggagaatatt 224220tgccctgaaa acgtaggcat gatcttttgt tattatttat tttaaatagc tgaaaaggct 224280gggctttctt gaaatgatgt atatcactgg cgcttttccg aaatttgtca gttcaaaact 224340aaaagtgacc actgactaat agagtcgagg cttctagtgt atagaaactg gagacaacct 224400gagtactcat gtactttata tttcagttgt caaacagttt aggtatctgc tttcatgctg 224460tttcaagaat caatgcaact ctgacccctg cctgagactt tctgatataa atggattcac 224520gtgcagaaac tatagaattt tattaatcag gctgatttta aggctattct ggaaatattg 224580tgtacataaa atttgttttt ttatttttaa attatcagct caggtagaag ttaatcagat 224640gtacagagca ttatctgttg gattctcacc taattaaatt ggttgccacg ttttgatttg 224700acctacagta gcagaagata caaaatgcat ctgttcactc agttcagaac taaagagtat 224760tcactttctg agcgttcatt aacaataata gaaaaaacac catagaggaa actaagcaag 224820tctttaatgt agtctgaaat ttactatacc cacagaaaag gcctagcaac atggcaccaa 224880gtccatgatt actgctaaaa caagagatac aacatgtatt attcttacag ggtcacactc 224940agttgagaca cttgctttta cttcagattt catatgaagc ttttgcctta gagatgtaac 225000actagagaga aagcacagac ttggaacgag gtgatatcga gtccccctga tccagctatt 225060tgctgcctag acgtcacctc tgagccacca cctggtcctc ccagccttgc ctatgtccta 225120catggcaagt tcacagcacc tgtcctgact actctgtccc tgtggtctgt ctggtcccca 225180ggtcatggct gtcccttacc ccatctgttg ggctgacttg gccttggttt tccaaatggg 225240ctttgtaccc acctttatgt cctgcagggc tgggggcagg atctctgcca ggttgtttcc 225300agacatgctg atgtttgcga gctgttgaaa ctttgcttcc tgctgcttca gccttgcggc 225360agcttcacca tgagcattgc tataggcctt ccagagctgg agcagactct gggccttctg 225420caactggtct gcaatggctt ggttcacctg agtccacctg tcagtgcaac gcaaataaca 225480taaccctcat tctctccttt ctcttgtagc gaaagtacaa tgcacacact ctactggtaa 225540gttactagca gatggatcat taagaagaca ctgttaaatg gtttttaaaa ttagtggcat 225600caccattata gaaaccatta tataaaaata aatccaaatt aggctattta cttgacttta 225660taatattgca caaaaactac aaaatgtatg attggcaaca aatacctatg atgttatgaa 225720tgtttcactc tcttttttta agagagggga gtgatgaagg gagctcaaac tttttttttt 225780ttttttttgt agagacaggg ccttgatatg ttgcccagga tggtctcaaa ctctgcagct 225840caagtaatcc tccaacctca agcaatcctc ctgcctcagc ctcccaaagt gttgggatta 225900taggtgtgag ccatggtgcg ccccgcctat gaatgtctct ttggcaaaga tggagaaaac 225960actgaactgt atgcctgata aaatgactcc acatgaagag gctcccagtt tatagagata 226020tgctatcttt aaacaggttt taactttgta tttatatcaa ataaaattta ttatctgttt 226080acacaaatgt tccttacata gaagtggaaa taaaggaaat tgttttatta ttccatatac 226140agactctagt atcttgcctt tcttccctac tttttttttt tttctgaaac agggtcttgc 226200tctgttgccc aggctggagt gcagtggtgc aatctcagct cactgtagcc ttgacctcct 226260gggcttaagt gatcctccca tctcagcccc cacaagtagc taggaccaca ggtgcatgcc 226320accacgcctg gctaattttt gtttttttgt agagatgggg tttcaccatg ctacccaggc 226380tggtcttgaa ctcctggact caagtgatct acccacctcg gcctcccaaa gtgctgggat 226440tacaggcatg agccactatg cctggcccat ttaactttta aaaaatattt tccccacgtt 226500agtaataaaa gaaaataagt aaacctcatt ttaagtggct gtacaaatgg tctgtgggtt 226560tttttttctt tgtttcctta gtttaattgc taaattcctt agtttaattc ctgtttagta 226620tggatagtta agatatttgt aatttttgct attatgataa tgctcagata cagttttatt 226680caaaaggctt tttttctgta tttcaagata ttagtcttag actagatttc tgggagaatt 226740acagagtcaa agaatataaa gatgtaaata tttgtaaggc tttgggaata aatggtcaat 226800ctcctttctg aaagcattat atttacattc taacatgaaa tacatgagaa tgtttgctcc 226860acaatatcct caacaatatc ctcgaccctg ctgattaaag aatcaaaggt ggaaaatagc 226920acactgcttt acttttatgc atttcaaaaa tcttatcaga actgtctttt aaaataagat 226980tttccctaat tataaataca acatatattc actgtcaaaa agcaaacaaa atccctccag 227040atatttttct ttttatctat ctatcctacc tacttaccaa tccatgagga agttaaaaaa 227100tgcttgacat ctcatttatc tagaggtaac tactattagc attttagtgt tcttctcttc 227160agtctttttg ttatgcatgt ttttcaacat gccttccaac atattcttgt gccaacaagt 227220gaatgtgtac gtgcagcgta acaactgagt tcctttacca acagactgcc ctgccatacg 227280ttttgacggt agccctgata ttgtatgtgg agtcagatgc atttgcaggt tcctcttagt 227340ctgtctcctc cccgtcttcc aataaagact ggatcaagag ctaagggcac cactgcaggg 227400tatataattt ggggtccctt gttcattact tgttcgaagt tgatttacaa gagaggctga 227460aactgcatac aggaataatc aggaaggaca cttaagatag cataactctt catccctatc 227520tctaatcaat tggttggagg ccaactttta tatcccactc atcaaaataa aagccctgca 227580attaactagg gccacactcc ttaaagggag ctttcatatc ccaccataag tagttcatca 227640aagaattcct tccaaaccca caaatgccct ccgcaacaga tactctaaca tcccctttct 227700tccaccttat aatgtttcca gtgatgtttg aacaccccct gcaggatgcc catctggccc 227760actcctttgt ctgtttctgc ctctgacttt ggtgtttccg agatcgaact tgacatttgg 227820tctatactgt ctgtaagtga gtacactggg gttgttatta ggccatttct agactaatta 227880taccaaacac ttgtacaagg gatgtcacag ccctcatgac atagaatctt acatagacta 227940gattcatcaa agcagaagag accagaacag taggaaacca gtcatctcag aggaggaaga 228000agactttaca atgcctatgc cccctcattc ttcacaagtc attaagataa agatatctat 228060cattcaaatt atatctgaaa gcataccttt tatgagtatt ttggcatttc tcttcgatta 228120tttcagcaac agaggggcag agacctttga gtttgccgat aacctcctgg agtttctccc 228180aactcccttc actgctctca gcttcatctt gcagagacta agagacacaa acaagaaatt 228240taagtttcag aatggcctgg ctacttccaa agaaggtggc agcttctcaa cacttgctac 228300tatgtagcaa taacactcaa atcttacagc tctgataaac tattaggaat gtgaagaaaa 228360caatggagaa ggcattcttt atttgaggct gaacttttaa gtactaagta atataaagta 228420cttagcactt aggcatactg aaaaaaaaaa ggccactcaa aaaccaaaac cttaaattgt 228480gaaataatat ggacatggaa aataaaggaa gattcaacat tttcaactca aaattcaaac 228540attttacaat gttttactga tttggctcat gtgacttatt ttggaatttc cagttattct 228600tttgaaactg ctatttaaca tgctttgtgc ttctctgtgg gagggcacaa acacagttat 228660gcccgttact gtgcttcctc ccatttgtaa tttcctgcca ataatgcacc atgccaagct 228720ctggttaatt tacctgaagg ttctccacct ggcatctcaa ggtctccaat gataacacca 228780caggcttgct gtgttccatg cagtaccaga atcggattgt catcatattc acttcatcat 228840agagttgatc ataaatcttc cactcctgta aaactgactg catggaggtt ttgagctgat 228900tgacctagac aaaaagtgga aacgtactga tttagaaaat cactgagaag acatgctctc 228960caacagatct accattgtgt ccaatttagg catttcaatt tgagagtatt gaaggatcac 229020cagtgattaa ctgaaggtgg aaagggtagg tcaatgcata agaacatatc agagaaggct 229080gttttataat gagtgaatga aaaagagccc aaagatcaga aggatcagca ctgaaggagt 229140tcagactagg aagagacagc ccagggaaag aacactgggt gagtattaga atttgtgagt 229200gcaaggttca gttatattct attagctttc tgaccttggg caacttattg gaccctagag 229260tcagtttcct catccacaaa acagagataa taattgaaaa cacaaataaa atagtgtctg 229320tgaaaatgct ctgacaacag cacaacatta taaatgtaag atgttacctg gcacttaagt 229380ctgattatta ctacagagca agcaccaatt ggagaggaac atgctctacc aaaatgatgg 229440gcaagtgcaa agcaggaggc aagcaatggt gaatgtattt tgtccactgg tatgttctga 229500taatgatgaa aaaagtccaa gcattctgtt gcatatccaa aatgatctca gatacaatca 229560agagtatgta aagaaaactt acaaatcaat attaaagaca gataaactaa ctaaaaactg 229620ggcaaaggaa gtgactaaac atttccccaa agaacatata taatggtcaa taaacatatg 229680aaaagacact gaacatatac agtcattaag gaaacctaaa tcaaaaccac aatgagatat 229740tatttcacat ccactttgat ggccataatc aaaaagtcag ataataatgt gttggtgacg 229800atgtggaaaa actggaatcc tcatacattg ctggtgggaa tgtaaaatgg tacagtcact 229860gtggaaaaca gctagatagt tcctcaaaaa attaaataga gttgccatat gacctaacaa 229920ttgtactctt atacacacaa gagaattaaa agcgtatgtt cacataaaaa cctgacacaa 229980atgttcatag cagcacgatt catgagagcc taaaaatgga aacaacccac atgttcatca 230040actgatggag aggtaaacta aatatggtat atccatacaa tggaatatta gccataaaaa 230100taaatgaagt actgtaaata ctacaacacc gatgatcttg aaaacattat gcttagtgaa 230160ggaagccaga cccaaaaggc tatatagcat atgattctat ttatataaaa cgtccagaat 230220aggtaaatcc ataagaaggt agactagtgg ctgccaggga ctggagaagg ggaaaagtgg 230280ggagtgactg ccaatgggta tggaggtttc tttttgaggt aatgaaaatg ttctgtaatg 230340agatagtggt gatggctgtg tatctctgtt aatatactaa acatcactga actatacctt 230400agaaggtgaa ttttatggta tgtgaattat atctcaattt ttaaaaagga tctgggttga 230460aatggaatgt gatcactata gtggctccac aggccatctt tatgggtagt gtgatgtgag 230520ttttgggtac aatcacacag ctctgcactc agaccctgtt ttctgtttaa taactacaag 230580gaatgctggc acatccacag tgacagggag gctcctcaat ctctgcccca aacagggctg 230640gataaaaaac caaagtggga cgctaaaatg tgagtctcat gttggcaact atgtggactg 230700aatgtcttta caagggcagg atggctaagg aaaagaaacc aagttattta gttaagaaaa 230760taaagaaggc ggccaggtgt ggtggctcac gcctgtaatc ccagcacttt gggaggccaa 230820ggtggttgga tcacctaagg tcaggagttc aagaccagcc tggtcaacat ggtgaaaccc 230880tgtctctact aataatacaa aaattagccg ggagtggtgg cacacgcctg taatcccagc 230940tactagggag gctgaggcag gagaatcttt tgaacctgga aggtggaagt tgcagtgagc 231000tgagatcatg ccactgccct ccagcctggg caacaagagc aaaactctgt ctccacaaaa 231060aaaaaaaagg aaagaaaaaa gaaaataaag aagaaaggca aggaaggaat tcctcatcga 231120tgtacatttt tgctagaaat gatctcacat ctatctgggt ggcagggata attctggcac 231180aaagtaccgc atcatcccag agcccatata tacatgtcaa cacaagaact gccatggctc 231240atgttgcaca aggcctttgc agcttccaca gataaaggtg gtggaacttc tagcacctta 231300aaggccttct ggccttataa taaagcttca tggaaaagac acagtacatg aggtttgagg 231360gaaaggatga gaaaatcatc aagtaaaatt cactatacta aaaacaagcg ggaggaatat 231420tttcaaaatg ttccaactgg ggtcatcagc agggcagcat ttattgagca ttgtataggc 231480atcagtatta agatagaaat aaatgactag gtgttatcat ccccatttta tagaccagaa 231540aatcgaggct tagagaggtt aagtaacctg accgaagata cagatcaaaa atggtgccgc 231600caggatcctt atttaggcag aaactgcaaa gcccttgctc ttaaacacta ggttgtattg 231660actcttgttt acccctgggc tcaagtaatc ctcccatctc ggcctcccga atagctggga 231720ttacaggtgc atgccaccac atccggctaa ctttatttac tttttgtgga gatgggatct 231780cactctgttg cacaggctgt tcttgaactc ctggcctcaa gcgatcctcc tacctcagcc 231840acccaaagtg ctgacattac aggtttgagc cactgggcca ggcctacatt gactcttgac 231900agcttataga tggatgatct gtgcctcacg ccttctgcgt tttttttttt tttactttat 231960tgaaatataa ttcatgtacc atacgattca tctatttaaa atgtgtaatc tccataatca 232020attttagaac attttcatga ctctgaaaag aaactgcata actattagca gtcactcctc 232080gttctcccca gccactaatc tgccttctgt ctttatagaa ttgtccattc tggttattcc 232140acgtaaacag aatcaaacaa tatgtgacct tttgtgactg gcgtctttca ctcaccataa 232200tgtttccaag atcatcagtg ttgtagtata tatatatata tgtatgtact acttcattcc 232260ttttgtatgg atagaccacg ttttctttat acattcatta gtagatggac agttgggttg 232320tttacacttt ctggctatca tgaatcatgc tatgaatatt cacgtataag tttttggcgt 232380ggacacgttt tcatttctct tgggctatct atggaggagt agaactgcta agttgcatgg 232440taactttatg tttaaccatt tgaggaactg ccagactctt ttccagctcc atggaacccg 232500tccagcagtt tgtgagggtt acgtctccac atccacatta acacttgata ctgtctgtct 232560ttttgttaca gctatcctag tgggtggcaa gtggtacttc tttgtggtgt tgattttcat 232620ttccttgatg gttaatgaca tggaatatct tttcaatgca cttttcggcc atttgtattg 232680tcttcttcac agaaatgttt attcagatca tttgtctttt tttttttttt ttttttgaga 232740cagggtctca ctgtgttgcc caggctggag tgcagtggca tagtcttgcc ttactgctcc 232800ttccacctcc caggctcagg tgatcctcgt gcctcagcct cctaagtagc tggactacag 232860gcacacacca ccacacccag ctaatttttt ctatttttgg tagagatagg gttttgccat 232920gttggccagg ctggtcttaa actgctgaat tcaagcgatc tgcccacctt ggcctcccaa 232980agtgctggga ttacaggcgt gagccactga gcctggcttg tatccatttt ttccatttgg 233040tcatttttcc ttttattatc aagttgtgat aaatcttcat atattctagg tgtaagtccc 233100ttatcaaata tatgatttat aaatactttt tttaacaatt tgtggtttgc ctttactttt 233160ttgatgctgt gctttaaaac atcaaggtgt ttagttttga tgaagtccaa tgtatctatt 233220tttttctttg atagcttacg attttggtgt cattctaggt aacccctgcc taatccatga 233280agtcatgaag atttacacct gtgttttctt ctaagtttta tacttttagc tctaatactt 233340gtgtcttttt tttttttttt aatacaaatg ggggtcttgc tatgttgccc aggctggtct 233400tgaattcctt gcctcaaatg atctcccacc tcagtctccc aaagtgttgg gattacaggc 233460ataagccacc atgtctggcc tgcatttatg tctttgatct attttaattt ttgtatattg 233520tatgaatgca gaggtctaac ttcattattt tacatgtgga tacatggttg tcccagcacc 233580atttttcaag aaagtgtttt ccctcattaa attgtctgag gatccttgtt gaaaatcaat 233640tgactacaaa tgtgagggct tatttctgga ctctcaattc cattccaagt atatatatgt 233700ctatccttat gacagtacta tctgcatgtt ttcacatgca ttatttcatt taattttctc 233760tttatatttt tattatattg taattatctg tttacatgtg tttcttccac tagcctatga 233820gttctttgag ggtaggattc atttattttc ttgtttacct ttgtaaccct ggcagtgagc 233880acagtacctc acacaatgta tgcttaacaa atgactgaat aagctcatct ctatttttct 233940gctacattcc tgggggaatg aggactgagg aatgaggctg agtgttgcct atggatttca 234000tgcaggttat tccctattcc ctcttggtac aaatggttga aagtagattt tagtaggtga 234060caacccacaa tgaaggttct ctaggaaaca tggacatatc tataattgat ggtatagatt 234120ataccattga tagattatac caattgattg gtataattgg ttagtatttt tagttctctt 234180cctctgttgt gggaaaatgt caaggttgca atgtctagag atgcctgagc tcgctctctt 234240ttggtgctct cctcctggaa ggggtaaaat gctgacattg acatgccttt tgtaagatta 234300ttagatgtct tgcacccttt cttcctttat actctctctt caacaccatg tagtgtttct 234360tccaaacttt agagtcctcc tggagctgac tatgctccta gctcatagag gctacatgat 234420acaggatcac ttcctccctg aggcatctcc ctgtacatgg agacactgct tcatcactgt 234480ttctgtatat cttgccagga aacaatgcca gatactctgt tgggtttggg gtatggagat 234540atttatgtct gagtgagtac attagacaaa acaaagataa ccaagtggat ttgcagcctg 234600tcaatgacgt tggaattggt ggctttgcct ctaattattg agtgcataaa atgggacttg 234660aaatgcttct aatttacttc aatttttctc agcaactttt cagtgtttgg tatagagagt 234720ctccatgagt aaaggacgaa aacaactaca acgacaaata aggtaaaaga atatgtgaag 234780agaaatagca catttcaaat gaattctagt acctgagtga gaacactgct tctcttttga 234840aataactcat caattcggga tgctgtatct tctaacaatg gcactgcccc actctccaaa 234900gtcagataac tttgtttcaa ctcatctagt gctttggatt taattgcaag ttgattttct 234960atatcctgga agagtaagag gaaaaggttt tcaatgcaca atattacaaa acaattaata 235020aggtgtcaga gacaggagtt cactcttttc aagtaaaaat gtcattagat tatataatta 235080aatagcatta catcaaagaa atttaaatca taatactctt agttcaatta catatgacac 235140aactctgtat tttaggagaa tctaggcaac acgtctgaac catcatggca gagcagaaag 235200gatgctggtc cgagatctgg tccccattcc acgtctgccc tgcactggct gtttgaccag 235260gcataagtca tcgaaccttt cagtctccat tttctcattt ggaaaaagtg atgataccca 235320cagcatgtgt gccaagctaa gaactcaaat aaaatcacga gcaagaaggt gcctttaaac 235380catatagcat catataaatg acagacgata taactgttgg tagttcagta ataactttaa 235440tcattttcta cctcttagtt tattaattca ctcataagag gcaccctgac ctggtctact 235500ctttatagat acatttttaa aaacctacag gatccctcta ctacaaagga caccatgggg 235560ccagcagtct gaaaacagag atgaatgagg ccagcagcaa tggtgccact ctactcagag 235620gaaggacggt cggagctgct tatttgtcct tctcaggatt gcagttgaaa tccaacttgg 235680aaaaagttta aactttttaa aaattattaa agcattacat gctcattaca aagaaactgg 235740aaaacacaag agatctttgg cattcagagg gattcatttt gaattcccct caagtctccc 235800aattcacaaa tttgggacgc gcttctgcat actttcttta gcattacatt cccatccaga 235860tgcatgtgtt ctgaatgcat ggcctcaaga taacgaccct tagctcatgt tctatttctc 235920tcttgaaaat tgctttgcat aaggatccca gcttttttta tttttatttt ttgaaaattg 235980ctttgcctaa ggatcccagc cttttttttt tttttttgaa aattgctttg cctaaggatc 236040ccagcttttt ttttgaaaat tgctttgcct aaggatccca gctttttttt tttttttttt 236100ttttttttaa atcctttgta agaatcactg catggtccat aaggaaagag aaacatgagt 236160gcctcgtggg catggggtag ctgagcaggt ggcttgtacc ctgggaggga caagctccct 236220tgtgctagcc tcacagagga gcactgaaat ccagtcccgc aaaactgcca gattaccacg 236280tgaggagccc tcaggtcact cttggaaggt ggaaatcaca aactctaaac caatgaatgt 236340caggggtctt ctggactgga aagtagaaaa agggaaaaaa atctgttcca taagaaactg 236400atagctaggt ttgggagtaa gaacggatga tgagagaatg gagctggagg aatcttccac 236460tgtatgaccc tctatgcttt tagaatttag agccacatga atatatagcg tatgaacaga 236520aaacctctca ctgtcgtaac aatccaccca tactaacatg tgggtgaatt tatgaaatat 236580tattcttctg gactggttac aaaggcaccc aggaaacaaa acgacttctc tgaaatagcc 236640tttgccaatg agatttagtc agctgctacc tctgtccatc tctgccaccc taactcgctc 236700cctcacaccc tgacagcctt atttattagc atgtttcttt tatcataggt ttaacttttt 236760tcatctgcaa agttataaat gaaaacactt taatattaaa tcactttcct tctttgaatt 236820tataaaacag tctctcctaa aggcttgact aactagattt ctaaaaacac ttatttacta 236880ataagctgtc ataattcccc tataatgtaa agaaaactgg taagaaatga ctacaaagca 236940gacaaaagga agtaacaaca cacacacaca cacacaccct tcaaaaatgg cagcataaac 237000aacagccatg ccgggcacag tggctcacgc ctgtaatccc agcactttgg gaggccaagg 237060caggtggatc acttgagccc aggagtttga caccagcctg ggaaacatga caaaactctg 237120ccgttacaaa aaaatacaaa agttagctgg gtgtggtggc acacgcctac agtcccagct 237180acttgagagg ctgaggcggg aggatccttt tttttttttt tttttttttt tttttttgag 237240agagaatctt gctctgtcac ccatgctgca gtaggcatga tctcggctca ctgaaaactc 237300cgcctccggg attcaagtca ttttcctgcc tcagtctccc aagtagctga ggctacaggc 237360atgtgccacc atgcccagct aattttgagc caccgcgccc agagtgggag gatctcttaa 237420gcctaggagc tcaaggctga agtgagttat gattgcaaca ctgcactcca gcctgggtga 237480cagagtgaga ccctgtatca aaaaaaaaaa aaaaaaaaaa agaaaagaaa aaaaaaagcc 237540atggcaatag cagcacacga acaaccacac acggtggggt tgctcgtttc ttttgctctg 237600cagaataata cggatttagc ccagtagtcc tgtcatctga ccttgcttca ctgaggccta 237660acagtaccct tcaagtttcc caactcccac ttcaccggca aattaatttg tcttacttta 237720acttccaatt tgctacacat aaatcagggt ggaattaaaa ataatctttt gctaattatg 237780gagattcagt agaatttgca agtcctattt ttccattctg tgagaaccag cattaaatat 237840ttttaaaata tctaaattcc tctgacttta tcacgaacat acttctttaa gaagctgtgc 237900ttgcttatag tatagaggca tcaaacttga gggattaata atctcatctc aaacctcccc 237960actctattca actatacaat tttccttgat aattataata tgtcaaacgt ggcctttttc 238020cagtcatcaa tggcttttag aagcccttcg gaatatttca atcctaatgt cttcatgctt 238080catcaactcc agtccttaac ttttctagag taaagccact ttagcaagct tacctgaact 238140gaagcattct gccctgggat aagtcattaa agtatacatc aatcattttc atcattttct 238200tttctttttt tctttctgtt ttgttttttt agagatgggg tcttatccag gatggagtgc 238260agtggcatga tcatggctta ctgcttgaac cttgaactcc tggctcaagt gatcctcctg 238320cctcagctcc cgagtagcta ggattacagg tgtgcaacac tatgcccaga taactttttt 238380ttttttggta gagactgggt cttatgttgc cctggctggt tttgaactcc tggcctcaag 238440tgatccttct gccttagcct cccaaatttc tgggattaca ggtgtgagca actgcacctg 238500gcctcatttt agttcttgta tgcaaagaat agtggaaaaa aaaaaaaaaa aagagaaatt 238560aacatttata gaaaagcagc cttttaggtc tattaataga tatattcagg caactattaa 238620gcacctatga atttcacagg cagtgacagg tataacaggc atgctgatgt gccagcctgc 238680cttgagaatc ccacaatctg gtagacgaca gacccgtgga cagatgagcc cagtacagtc 238740tctgacacat gatgctacat ggggtgctag ggaagcctgt tttatttatt tgcttccatc 238800ttggccttgt ttatggcctt ttacaaagag gcatccaagc agcagcataa aaaattaaaa 238860taggaagaaa tgagctaatg gaaatgtggg agtaggtagg aaggcacagt cagtgggcac 238920gtggtgaggg agggggtcag ggtgctttga ttgagttctg acaaacatag aggagaacct 238980gtgtgtggat ggtgggggcg aggggaggga agtagaaata attctgcaat cagctgcaaa 239040tgtaatggca cagaggcaaa tggggactga cgagggatga tgccggccag gcatgtctgt 239100aaatttcttt taaagcccct tgtctcagtc aacactagtc atttttctaa tgagcagagc 239160aaatgtgaga aatggttttc tattggtaaa attaatggat tattattgtt attagagatg 239220gggtcttgct ttgtcaccca ggctgaacta cagtggcatg atcatagttt actgtcacct 239280tgaattcctg ggctcaaagg atattcttcc cttagcctct tgagtagctg ggactacagg 239340catgcaccac catgcccggc taatttttaa atagtttttt gtagagacgg ggtctcaccg 239400tcttgctcag gctggtctcg agctcctgga ctcaagcaat tctcccacct cagcctccca 239460aagtgctggg attatacaca tgagccactg cgcccggcct taatggctta ttaaataaca 239520taatgagtat tagaaatggg ttctcactag taaatttcat gtactcaaga tccatggatt 239580ggctaaacta ccgtagatat aatttccacc cctacttgaa cggctataag aactgggcat 239640agcctgttgc cttcaactag tgcaataatc cctaaggtaa gtccttatgt ggtttttgta 239700tccagtttaa actccaagga ttctggttgc ctaatgttgg ccatagaaat aatggactct 239760tgcttctgga atattactat gtctgccttt catgcaaagc ggtggttggt gtgctaaaga 239820gggcagagag gctttgctga acttagttat ataaactatt tttagtgctt gcttgctaat 239880ctatgtttct acagatggtg gcattgtgtc tggaatttat tttatttaca gcttggtagt 239940gaggttgtgg gaagaatagc taaacttaat ccatttcgac cttgttccgt ggctaccatt 240000atctatcaaa aagagaaaca actgtgtaag actcacttga cttaggtcta tttgcaactc 240060tttgtaggtg agggaccaga atgtcattct aaaacatgtg aacttcattc tgtggtccat 240120ctggagccac tggaggattt tccagtggag agtgttgtga ccctttgcat ttcagaaata 240180tccctctgca gcaatgtgga gaatgaatca gaaggaaatg agggaggagg ctggcaagtg 240240ggctggaagc agctgcatga gacaggcctg cactgagggt ggtctgaagg aagccggcct 240300gaccgagaca gactggccag acagtaagga gtaagtcaag agcacctaat gagaaactgt 240360atgtagcgac acgcatgaaa acacctcctg ggctctggaa agaaccacct actaggggac 240420aggccagcac caggctgcgg aatgtaggct tccagtaagg aagcacatga ctccaaggtg 240480gctgtcagaa ggctaaagtg gagatggctc attgggaggt gggaacagta tacgagcagg 240540tgaaaagggc cgcttacggt ggtgctgctg ctcacattta tgtccagtgc tatcccgtga 240600cctcctcagt agaaatgggg tccggccaga ctttccagtc ctgacagtga ctattgtaag 240660gccgtcacgc aggaagtgca gaaacagcac agcgtgtgcc gcctgccgtg atggcgatgg 240720cagaggtcct ctgaatgggc agcagccaca agggcagtgc taaagcagga tcagtgtgca 240780aagactgagt gatgcggcaa agcatagctg aaccacggac aacaccctca agcccaaatg 240840tgattcagca gaggttccaa gggacaagtg aactttttaa agcataatac acaataggta 240900gaagaaaatc attttcctcg aaaaaacaca gaatgaaatg ctaaaaataa tgaaaccttt 240960agagtaattt tgtaacatta ggcaaaatga ttaaacaaat ggaaaattgg aaagcattta 241020aatcaaaatt ccctttgaac atttttcccc aagagtacta ctcaaacctc ttaagagcag 241080caatactgta acggtgaaag tacattcttc tgtttatcat aaaaaacggc actttgcaat 241140tttgtaaaac ccatgggtca ttatctaatt gacataagaa aataagtaaa tagaggggag 241200ctggaagaga gtttccacaa actacagcaa cttatcctcg gggcaagaga ggtgaccgaa 241260tcaccccagc tcgcgggttg ggtgggatgc tgttcccctc ctcacctgac agtccaggag 241320cagcttctgc actgagatca cactttcagg tttttgtaaa gttttcagtc tctcttcttg 241380tgcttccagc cagttgttca agatctgtat tttattttca ctctcagtga tactttctag 241440aagttgttct aaatgttgta tctaagtgaa tgtaaagatt acaaaaaatg ttaaaatgta 241500tgttttaaaa ggtaaatgcc atgacgttat tttttaaaaa acaattttgc ttcttttcaa 241560atttctaata tttcatgtaa taagatgctc tgataacttt tgaataaaga ttcaaaaatc 241620agaaaacaga gttaaagctc ctcttaggaa aaaaatatca tatttaaggt gaaagctaat 241680ctgctgagaa aggtagctgt tgtggagttt cagatgtata caaaataaaa tgttcccaac 241740attcgatatt ttttcattgt gtcatctatt aatatatgta aagtataaga caaatttctt 241800tcctcagact ttctgcatta gagttgctta aaatttctac caatatcaat aaaaaataat 241860ttatcaattc aataaaataa tcattgtctt taatgtctgt gattctcatt cataataaat 241920tttttattag gtcataatca taatatacac acatttttat attctttttt acatacatta 241980taactactgt gtcatgacat aatttttttt tttttttttt tgagacggag tctcgctctg 242040tcacccaggc tggagtgcaa tggtgcaatc tcggctcact gcaacctcca cctcccgggt 242100tcaagcaatc ctcctgcctc agcctcccta gtagcaggga ttacaggcac gtgccgccat 242160gcccagctaa tttctgtatt tttagtaggt caagaaaaca cctgaccttg tgatctgccc 242220gcctcggcct cccaaagtgc tgggattaca ggcataagcc accacgccca gcctgtcatg 242280acataatttt aaaaggctgc ccggccaggc gtggtggctc ttacctgtaa tcccagcatt 242340ttgggaggca gaggtgggtg tatcactgga ggtcaggagt tcgagaccat cctgaccaac 242400atggtgaaac cctgactcta ctaaaaatac aaaaattagc taggcgtggc ggcaggcatg 242460tgtaatatca gctatttggg aggctgaggc aggagaattg catgaatctg ggaggtggaa 242520gttgcagtga gccgagatcg cgccactgca ctccagcctg gggaacaggg cgagactctg 242580tctcaaaaaa aaaaaaaaaa aaaaagctgc cccaatgttt catcaaatta atacagtata 242640attcgatgaa ctattcccct agagtgagaa ttaaggcatg gtacattttc acagctgtaa 242700acgatgctga attgaacact ctgtgcacat catttacttt tttgaaggac tatctactta 242760aatgtgtaag aggtgacttg cttggttaag tgataagatt attattacgg ttctttacat 242820tttccaaatg ctttccaaga aagtacttaa gccattatca gcaatgtatg aacgtgacag 242880ctttgggttt ttgtccttat ctgattaatt ttttttctaa ttaagtgcat tatgttacac 242940agtgtttatt ttgagtatct gctaatgata tctttccatg tttatttgct aactataatt 243000tcctcttttg tgaattgtct gttcatgtcc tttgtccatt catgtacttg tatcttaatt 243060gtgtaaatcc atttgtatgc attctctaca cagtaaacac accaattatc atcatgatta 243120tatgtaaagt attttttcag gaataagttt tccgaatctt ttcaagagtc atggagtgaa 243180aaatctacca aaaagccaag tgcatttttc tttcttttta taaccttttc aaagctgaaa 243240tagctttgcc ccttttgaac atctgctttc atagaaaatg taaaatctct gatcatttag 243300gagtctgaca taatacttga catgttccta aataaaattc ctcatcttca cttgcctagc 243360tcaatttccc actaagaaat ctcaaatata agaactattt ttatattctc ttataggtac 243420caatggagaa tgctaaaaca taggatttat aaaagggcta tagttgagat gattattagg 243480cttaataatc agtcaactaa ttgaacgtct tttttgattt ttcagaaagt gtgtttcata 243540aaggtcaaac cacacatcca gttgtgacgc aggaacacac ctttctattc agcattccat 243600gtacacggtg ccactggcgg ttcatctccc ccaggtgctc tgcaaactcc gttctttcat 243660agcgcttgct ttctacatca caggtgctta gctgaagtaa tgactggtta acgaagtcaa 243720ctatccactg tttatagtcc atttccattc taaactccta aaataagcaa agttaaacac 243780caaagcagtc atgaatgttg gtcagatcaa atggatttcc ctttatgtgc tcgagggggc 243840accaagctcc cgtccattaa tcaaggggca cctgtcagga gttctgttct accctcccca 243900acctacacca ggagttggct ttattggagt agggattctt ttggggaatt acacagagat 243960gcactgcttt caaataggct gaaaagtcaa aggggcacta tgtatggcag ttgctatcat 244020ctgaaccctg accacgaccc aggatatgac aaagcttcct gggggaggct gcctgtcagc 244080tgtgctggaa ttctgggcca tcctgagggc tcacggaaac aaccagatgg gaaataggaa 244140gtaaagtctc tttcagaaaa cccaggacac cttaaggaac tctgtcattt aggcagcttc 244200cctgttcctt cggggatagt taaggcctct caggtggctt tagaaagttt gggtggcttt 244260aagttgtaca gcaatcctaa acccacccag ggccaacaac atttctgcca aacctgtcac 244320ttactgattt tatctctgca agttctagca cctcttttgg agacacccgg gggtgacagt 244380catgtggtat gtgctaggtt gctagagcct gtgagaatat acacgtgtag acttttcgaa 244440gattttacta cacactgaag ttaggatgtg atggtacata aaaacaaaat tctctgctaa 244500tatcttgctt ggtgattaag ccattcatct tctgcctacc agaagctagt tccatcagcc 244560tgcatggtct taaagcaatg accacacttg tcactcatct ttgtattccc agtggccagc 244620atagtgctga gcacacaata cacgtttgca tgtggctcat gaaatgttaa ttaaatctac 244680ttgaaattaa atatcacaga gaaggccaca tttatatcta tttgtagaag gatctcaata 244740ttttcttact cattctctta ctcaaagaac atttcattaa cacctgttag aggaaggcag 244800gtattgggat aggcgtgcta tattttagca aggtgaagga tggcccctgg tctcgagaag 244860ctcagtcaag gtggggagac aggcaaataa accaataatt ataataatgc atgataggag 244920ctactaatag catcatggag acatctaatc caggaaagtc acctagtctg gctgggagag 244980tcaggggtgg atttgcaaac aaggacaact cgagctgact atggaagatg actaagagga 245040cgtatacagt taaacgtggg ccctggcatt ccaggtagag ggtgcagcat gtacacagga 245100tgataaggga gagcaccaag aacagttaag gaatcaaaat cactctgtca ggcagagtct 245160gtggttattg ggggtggtgg gatggggata aaaaggtgag accacagtct gttaaaagct 245220tgcttggatt gtcctgtggt gatgggggct ggcaaaagga atttaagtgg aacaaaagca 245280agtctactaa catctctcca gaatcatcca ccaccccagt ttcctacccc tagaattagt 245340tttttgaaat tatatcctat gtgtcccttt gtccagcatt acccctctga ttagccttct 245400tgtttcaatg aaaagacttc tgctcctttt gcataattac cttgtgcttc tgaagaagat 245460gtttaacttg agatgcagaa cttggtgaat gcacggagtc ttcatctgaa gtttgatgct 245520ccacattgtt catccagcta atcatttctg tgattgcttt acgagacggc aatttctcca 245580tttgaagctg taagaacaaa atgatttcca tttaattgcc tgcagttaac aaaatgaatc 245640agagcatcac taaagatgat atctggtttg ataaaaggtc ttgctatata attatgcagg 245700ttactcaaaa aaaaaacaaa cccagcattc aaacttagaa taagcgaccc caaaacaatg 245760acaatgacct tcacataaac taacacagtg gcaaagataa actgagaaat aaagtaatta 245820agcctgattt ctctcttaag aaaaatccca ttggaggagg gtccagtaat taagacttat 245880attcagtggt gccagagaaa aataggtagt gtcaatgaaa attattgagt cagagaaata 245940attttaaaag agttatgagc taacaagtga atgctaattc aaatgctgag gctctaaaga 246000cttacctggt gaagtttttc ttgaatatct ggaagttgag ttatgagcat tgtccatttt 246060tgttcaaact gtgctaaaga agctctcagt gtagctgtat cagtttcttt caggtgaaga 246120agctggttcc cgatactgat aacggcagtc ttcaaggagg atttttcatc aacttctttt 246180gaaaactcct aaaggagagt tttaaaattt agaaacttaa aatttaaata tagcagaaag 246240taagagtcgg aataactggc tagagtcaac aaataaacat agtaaatata cgaatgtgta 246300tacacacaca cacacacacc cctttttcct actgttgggc attacataca cgatggggaa 246360caaaagagat ggaatgcata cccctcaaga agcttatcac ccagtgggtt aacattttta 246420aaaataagtc attatttaaa tggggtaagt tttacaataa actatggaac ccaacacaaa 246480gtgcacatta aatgaattac ttgcaacaag tttgtaatta aaatgtttaa atctttccta 246540tgtagaaatt ctttcaagta tgttgaaaaa atttacattt taatagcaaa gttagtaatc 246600atagctacga agtatgaaaa ataaaaaaaa aacaggaaaa gtctaacaga aaagactacg 246660tttcataaat gcattgacca ataatcattt tgtaattata ttacaagagt aatacatttt 246720tatttccccc aaaatttggg ggatattttt gaattttttt ccaaaaaaca cctcaaaaca 246780atggaacaaa agctttttag tagaaatatc aattttgttt acaataattt aaaactatgc 246840atttattaat gttaacattt ataagttaat aaccttataa agttgttttt gggtcttgta 246900ttttttgtat ttgtaaaaca tgacctatgt cagtgcaagt tttttgagac tgagtttcac 246960tcttgatgcc caggctggag tgcaatggcg ccatctcagc tcattgcaac ctccacccac 247020cgcggttcaa gtgattctcc tgcctcagcc tcccgaggag ctgggattac aggcatgcac 247080caccatgctc ggctaattct gtatttttag tagagacagg gtttctccat gtttgtcagg 247140ctggtatcga aatcccgatc tcagctgatc cactgcctca gcctcccaaa gtgctgggat 247200tacaggcgtg agccactgca cctggcgcaa gttttaaatt cagatatatt ttatggggtc 247260aattaaaaac atattttatt ataaacgaat gaacagtata aacagttcta ggtttgaaga 247320acacagctgc cattcgccca agtcttaccc cttatccaca aataaatacg aagactatca 247380catcaaccct gatgcttttt ccttgatatt tatttctgac cagtaattaa ctgaacatat 247440gctattacaa cttacaaaaa aattgttgat gttgcttctg attgtatcca agtcctgaga 247500cacattgagg gactgttctt tccagtaatt cagagtatgc tgggaagatt ccaaccactt 247560ggttaactga tccgaatctc tgttataact aacaggagca aagtgagaga gaaggcagat 247620tagaaacata ttctgataat gatatgcatc accagcaaat tttcttattt tattgtatgt 247680cctcctatgt tgatcagtcc taacattcag aatatcatga cgttaataag gattatatca 247740tgttttatac catgatgctc ctggcatttt ctaggactct cttcattaca gctcagcaag 247800aacagtgagg attttaggcc agatgtggtg gctcatgcct gtaatcccag cactttggga 247860ggccgaaaaa ggtggattgc ctgaggtcag gagttcgaga ccagcctgaa aaacatggta 247920aaaccctgtc tctactaaaa aatacagaaa atacccaggc atggtggcag gcgcctgtag 247980tcccagctac tcaggaggct gaggcaggag aatcgcctga acccaagagg tggaggttgc 248040agtgagccga gattgcgcca ctgtactcca gcctgggcga cagagcaaga ctctgtctca 248100aataaaaaaa aaaataataa taataatgag gaagtctaaa gcattcttta tttcttatta 248160cagctacaca aaagtatacc caatgaagga acagagattt ttatccatca aatgtcttct 248220ataaatttgt ttatattaaa aatataggtc aggcacagtg gctcacttct gtaatcccag 248280cactttgggg taacaaggtg ggaggatggc ttgagcccag gaatttgaga ccagcctggg 248340caacatggaa aaaccccatt tctacaaaaa atacaaaaat tagcagggcg tggtggcatg 248400tgctgtggtt ccaggtactt gggaggctga ggtgggaaga ttacttgagc ccaggaggtc 248460aaggctgcag tgagccacga tcacgccact gcactccagc ctgggtgaca cagtgagacc 248520ctgtctcaaa aaataaataa aattacatac acacacacac acacacacac acacacacac 248580accatacata cacacacaca cacacacaca cacacaacca gcatttgtat aagctgtata 248640acaacaaact gctgctggct gaacacccaa cgtatttgcc cgaggtagtg aaaatattca 248700aacaatattc tgcccttctt gtaactttta aactgcctta gattaagatg cagggatgag 248760aatgatgttt aaatgttcta tgttctcctc cttcctcaca cggttcaaga ttataccaac 248820taagcacaac aaattctcca aagaactcaa aagcctattt caaactattc tgttagctaa 248880aatcatttaa tttttttaat tttaaaactt ttggaaagag caatatcatg gtataaaggt 248940aacctatata ttattttata acataattcc tagcttctat agaaatgtgt aaactagctg 249000ggtgcagtgg cttacacctg taatcccagc actttgggaa gctgaggcag gaggactgct 249060tgaggctagg agcttgagac caccctgggc aacaatgtga gaccccatct ctacaaaaaa 249120ttttataaaa ttagccatgc acggtggcct atgcctgtag tcacaccaac ttgggaggct 249180gaggcaggag gatcgcttga gtccaggagt tcaaggttgc agtgagctat gatagtgctg 249240ctgcactcca tgcactccag cctgggtgac agtgcaagac cttgcctcct taaaaacaaa 249300aaagaaaaga aaagaaaaga aaagaaaaaa cgatcacagc aaactgatta catgttatac 249360tgtgtctagc aaaccatcag agattctaca tcgcgtttag cccagaagag ggacccaact 249420gctttagact gcccaagaaa aaacagcata aggtgggttg caggtaaaat ttagttgact 249480acagaatccc cgaggtcttt cttcacagca caatatgaag ccaatccacc cccaaaaagg 249540ctgacctgag cagatgcttg agaagagctt gcagcctgtg gagctcatgg tcaattttct 249600tgttcaggga caaccactgc tcttccagtt ttgcgatctg gccctctaat tcaggacagc 249660tcacagacgc caccaactgt ttgccttcgt tcagagtttg gtaaagccgg gcgtgttttc 249720caccaatgtt tctttttatt tgctaataaa aagtaaagtc atagaatgaa ttatttagaa 249780aataaagtct gccaagctat tcacaaatac tgcatttaaa actgacattt gtgtccactg 249840ataaaacagt aacactttcc caacattcaa gactatatag tctgactgga tgcatgatta 249900atggtttatt ggttaccttt catgataggt gtccaatctt gcatttctag agcacagatt 249960caactttgta gacttactgg ctggatgcag tggctcatac ctgtaatccc agcactttgg 250020gaggctgagg caggcagatc acctgaggtc tgcagttcga gaccaggctg accaacatgg 250080tgaaacctcc tctctactaa aaatacaaaa aattagctag gtgtggtggc gcatgcctgt 250140aatcccagct acttgggagg ctgaggcggg acttggtggc gcacacctgt aatcccagct 250200agccaggagg ctgaggcagg agaattgctg gaacccagga ggtagaggct gcagtgagct 250260gagattgtgc cactgcactc cagcctgagc gacagagcga gactgtgtca aaacaaaaca 250320aaacaaaaca aaccaaccaa ccaacccaaa tcaactttat agacttattg catacaaaca 250380aaaaacaagc tggcagtcat taggccatgc tgagctcaca gataggtttt gtgtgacctt 250440aagatcagtt tgaaaaatca ggaaatctta cctaataatc aggattgcca gtgtctgtgg 250500gaaaactgga agacctagca attgttggtc tgcatctcat gtggcaaagg gcggctgtaa 250560ctgagcagca gctgctggct ctaggagagc ctactccctc tccttttccc ttggctcagt 250620ccccaccacc cctctgattg cagagccagt agggtttcct tatttctatt ctgcccccga 250680aagcacttca gatggtgact tagatatgaa gaggataaat aaaatagtat ttctctatta 250740tccatgagat gcgaatacta ggcagaaaaa ttcatttcca gggtcttcta ctgaatataa 250800agattcagta gagagaaagg aagaaaactt tagaaaaata aattaaaaca gcgtatcatt 250860aatccaactt cacggaaaga aaaatgaaat tctttgaaga gaagtctgtc taaaggtaat 250920tatctaacac agctggattc agctaatgcc atttatcctg acacctgttt aatcttaaaa 250980aataaattat taacaaaaca gaaggtttgc tattttcaaa ttaagttaag ccttttaaat 251040cattaccaaa aaattccacg tccatagccc tttccaccac tcccatttgt aatcccctcc 251100cccccgtgtc tgagcatctg tattaaaaga aaaaaaaatc agtctaattt tctaagtgaa 251160gtgctagatt cttcaccttt aaaacatctc tgaaaagtct taattcaccc cccaattttt 251220tttttttttt tttttttttg agatgaagtc tcgctctgtc acccaggctg gagtacagtg 251280gcgctatctc agctcactgc agcctccgac tcccgggttc cagcgattct cctgcctaag 251340tctcccaagt agctgggatt ataggcacgt gccaccacac ccagctaatt tttgtatttt 251400tagtagagat ggggttttac catattggct aggctggtct caaactcctg acctcaggtt 251460atccacccac ctcggcctcc taaggtgcta ggattacagg catgagccac cacgcccagc 251520ctaattcacc gattgtatgc atgggataca gtctgtgcac gagtaactag gctgagccag 251580catttgaaca caggtttgtt gacttcaaag gccagacaac tgactcctag gttccatgcc 251640ccacagacga cggctgatca agatgacaac atctgtcaga ctcacacaaa cctttccttg 251700attctgaact gagaatggcc tatgaagtcc atatgtgaga tgggctttac aggatatgtc 251760ttctttagtc tggcaaattt atttaaaagt cttagaacaa tttttttctt tttaatattt 251820caaatgtaat agaatcacac attttaacag atatggtcac ttttggagaa aacaatcaca 251880ctcatattag gttggctaat attagactgt tgcttaaaat aataagcctt aaaatactgt 251940tctcatctgc attctttcag ctgtttagat gataccttct gtatccctag actaacaaag 252000agctaaacag tactctcatc acccccactc ccacccaccc cagaggactc agatttacct 252060tagagttagg ctcaatataa ttaataaggc ggcaatgaga ccaactgtag ctttccaggc 252120atctagggag tataaataca gtgtcccagc ctctggaaaa tgttccttat agaggttaaa 252180atacctccat ggaaaagaga accctggaat tatttcctgc tttcattttc caactagttc 252240attcaggcaa taaatgtgct tttgaggttt taaagttgat atttcataac tgcctctagc 252300cgcacaactc agattctgac ggcatatatc ctaaagttaa aactaggaat tttaagttcc 252360tggtgagaag acttaaaact tgtttttatc ttgttggtca tgttctcaat gtcacttgaa 252420aactattttg aggttttatc ttttctttcg caccaaaatt agagacacag agacagatga 252480taacaacgca aaaagttaca caaaaaagtt taaaagtttg agggtttttg tttttttgtt 252540ttgagatgga gtctcgctct gtcacccagg ctggagcgca atggcatgat cttggctcac 252600tgcaacctcc acctcccagg tttaagcaat tctcctgcct cagcctccca agtaactggg 252660attacaggca catgccgcca tgcccagcta attttttttt tttttttgta ttttagtaga 252720gacagggttt caccattttg cccaggctgg tctcaaactc ctgagcccag gcaatccacc 252780tgccttggcc tcccaaagtg ctaggattac aggtgtgagc catcgtgtcc ggcctgagat 252840gtttttataa tgctggtgac aggtaagatt attgtctgtc ttatgggcta cgtctagtca 252900ggacttcact gtaggaataa aacgaaatat atgtttagag gccgaaggct gacaaagttc 252960tttccattct tcagctcatt tccatactag tagctcactg gcattcactt ccagagacag 253020gtagaattgg ctcgattatc tgcattccac agataaagaa attgaggctt agggacagca 253080aggtcacaga agtgataagt gatggagcca cgactcaagc aaggtcctct cccccaagcc 253140agacatgtgg ttatgctaag gagttcacat tcaaatctgt agtctccctg aatacattca 253200acagtgagtt gtatgatgaa cattttgttt caattcaaac cttccaaatc aagccactta 253260gtcaaaacaa atgtaaaaca caaagtgcta tggacacgcc tttgataact taaatggaag 253320acaaatacct ggtaaaatga aatcctttcc actaatcttt cctctgtttc ttccaccaaa 253380ctcacagagg gcaatgtaga tataagaatt tccaggtcct tttcaagaga tgcatagttt 253440tcatcaaatt cttcccattt ctagagaatc aaggacataa aattagtgga agccctttac 253500cagattggtt ccagataaca acataattaa tcacaaaaca aaaatgaaca ccaagaccat 253560acaaaattaa agcacagatt ccaggtgtgt tcgagagacg taaccccacc ctggtaagcg 253620ctcagcatct aatggaaaag aattccatca cctttgagga caaggtttaa aaagaatctg 253680agttggcatt tagtgtttta tgcttgtctt gtcaaactgg gtaacatgac taaaggagta 253740atacaatgaa gttaaaatga tactcttagc aactccgtag agaccttgga gccacaagag 253800agcctttctc ttattaggaa agggaaagag ctaactttta gacccttact caacagaagc 253860tattaggctg ctcctgctct ccaagcccac gcccaagccc accaaacccc catgccactt 253920gcagcatagg tctgctcaag gtgtctttga ggagtatagg cttacacttc atcaggggag 253980gacaactgcc ttagccctag tcttgtcatc ttttagcttg ccccgtttcc atggatgccc 254040accccgagga ccccaacaca ccacagagct gagttattga ctccaaacat tgtctgttag 254100gaaaagaacc acctcactct tttccatatc tattcacata gtggctcact tggcttttat 254160agaacactca ctgggtccat ggagtctttt ttaaaaaata ccatgacata ttataaatat 254220caacttgaca cactaactag aaaataaatt ttgttagcaa aaaaaataac accatattac 254280actatcaagt taaacatttc cttccttttt tctttttttt tttgagacag agtctcgctc 254340tgtcgcccag gctggagtgc agtggtgcaa tctaggctca ctgcaaggtc tgcctcccgg 254400gttcacgcca ttctcctgcc tcagcctcct gagtagctgg gactacaggc gcccaccacc 254460acgcccagct aatttttttt ttgtattttt agtagagacg gggtttcact gtgttagcca 254520ggatggtctc gatctcctga cctcgtgatc tgcccccctc cgcctcccaa agtgctggga 254580ttacaggcgt gagccactgc gcccggcctc cttcctattt tatttccttt aagtactaac 254640attggctatg aaaggtgtga ggggaatgcc tcaaaactga gaaggagctt tatgcatgct 254700tatttttttc tttgagttta gacaagattg tcatccattt taggacatct gggcctgttc 254760tcttgaagaa gtacagtttt taaaccaata actgtttcac ccaaatgatc tcattcaagg 254820tctcaaagtg cttatcgttt tgcttattca tacatgaacc taaaaggcag agagacgcag 254880taccatcccc aatctatgta tgagaaatcc cagccaactt ttccttcagg gagcggcctc 254940agaacctgag caggggtcca cattcctcac tgtcaggctt ccatttaact atagcttaat 255000gataactaga tacttcttct caacacctgg ggtggtgatg gaaaatcagc aattcccagc 255060ttcagtacaa cgtttacata acccaaatac ctgcaacaaa ctctgcagct tcataccaca 255120ttggcgtgac ttttcttcta gtattttaac ttctgttact tgttctgccc aaaatgtctc 255180tctgttttgc aataaagaag gaagtatttt ggcagagtat gcttggatca acaacatgtc 255240agcaacaagc ttctggaaaa aaacctatga gatggagaaa gttttaatat ttactttgaa 255300attgtgaaaa ctcttaaaaa ccaagttact tcaactttat atccagttat ttttgtttta 255360aatccaaaat cccaggacat ccgtgtagag tcaaattgac cagcctggcc aagatggcaa 255420tacaaaaatt agctaggcat ggtggtgaat gcccgtaatc ccagtcattt gagagactga 255480ggcaggagaa tcatttgaac ccgggaggcg gaggttgcag tgagccgaga ttgtgccact 255540ggactccagc ctaggtgaca gagcaagact ccctctcaaa aaggaaaaaa aaacaaaaaa 255600caaaaaaact cagtctttga acactagtgg aacttcaaca aattataaag tattaccctt 255660aggtgttatt ttccactaaa taatccattt taaatgacaa acatttttgt caatttcaat 255720ttccagatag atctttctaa atcagttgtt ttgagtctaa atgctacaaa aagatagatg 255780ccagctaggt gtggtggcac acatctatag tcccagctac tcaggaggct gaagtgagag 255840gattgtttga gaccaggatc tcgaggctac aatgagctat gattacacca ctgcactcca 255900gcctggaata cagagtgaga ccgtgtctct taaaaaaaca aaaacaaaaa caaaaacaaa 255960caaacaaaaa acagatgtca ctgtggctga tgaatttcaa gacaagttgg atggacagat 256020gtgcaaactc aagcaaccca catattcgaa agacaggtct ccttaaccct ggctttaact 256080tctactcaag agaatgacta aataaaggga atttgctgct ttataggaat cctggacata 256140aagcttcctg tatttgtatt tgaacttcaa gaacctttca agaggaaggc tttatttcac 256200atggttcttc tccatttaca gttgtctgga gggatccctc catattatgt gaatcccttc 256260tcctaagcct tgaaaaggac atatacatag agacgtttat aaaagcatct agcaaggggc 256320ctagcacact gcagataatc aatattattt tctcctcttc ccctccatcc ttcatacaat 256380atttaccttt caatttttct tagactaagc tctaccttcc aaatgctaga ccataagctt 256440acctcaggta ggaaccgtcc tacacatttt taattatcag cacctagatt actctttatc 256500tttagttggt gatcaagaag tttttgttta atgaaatgaa ttttaaaaat caaaggattt 256560actatagtct tatactacat aataacacac tttccttttg attctagttc tcaggaaagc 256620cttttcttct tttttttttt tttttgagat agagtcttgc tgtgtcgcca ggctggagtg 256680caggggcgtg atctcagctt actgcaacct ccgcctcctg agtttaagca attctcctgc 256740ctcagcctcc caggtagctg ggattacagg tgcctgccac catacccagc taatttttgt 256800atttttggta gagacagggt ttcaccatgt tggccaggct ggtctcgaac tcctgacctc 256860aggtgatcca cccgcctcag cctcccaaag tgctgggatt acaagcataa gccactgcgc 256920atggccagga aagccttttc taactttcct aagtcacctt ctgctattaa atgctctcat 256980agaatgtatc ctcattttaa ttttaaattt gtttgaagac ttgttgataa atatctatct 257040tctcaatcag gtgaaagctc tgtgaatgta aggaactgtg tctgttttta ctcataaccc 257100aaattcctag catgtaccag ggttcctggt acacattaag cattctaata gatgtttatt 257160aaaagaatga atagcttaaa tttcaacaaa gaagaaaaat ggatcctttc tgttcacatt 257220cagaaatacc ttaagatttt agtctatgaa ggggtcaccc gacccatgtg tctgtctcac 257280cttgtgattt tctatttgag cctgtaacgc cactttactt ttggtttgtt ttaagtggcc 257340atgagcaagc ttttccttcc caatcttcac cagttccacc atgccttgca acaaagcatc 257400ttgctggctc tcagtgacga aagggctgct gagttctgtg tacctggctc ttagcattcc 257460ccacatactg tcaagtacat cctgaaaaga gaagagcagg tgcacacatg cattttcatc 257520caaccggggc aataactgaa agacaaatct aaactctgct ggtggaagaa aaggatctgg 257580ctcctcacaa tcacagtgct gagtcaatgt tttctgctca ggcatacctc taatttgtaa 257640acctcctgga gtaaagcata aggtaaatga agcctctccc cttcatctct tagtttggcc 257700actcggctct cggcgttctc cagctccact gtatatgcat ctgctttcta agtgagagac 257760aatacaagaa aagaaaaccc ttcagtaagt cagagaagta cacatagtta tctaaaaaga 257820attaaatagt acagaaaggg atgaagggaa aagcaaaaat cccagctccc gctccctcac 257880ctcctgctct ccttaacaat cttgccagag gtcccaagtt cctaacatgc tccataccag 257940taggggtaga catgcatatc cctaaactca atgaactgca ccacagcatt ctgtcctgcg 258000gttagcattc ctcactcacc agctcttcag catctcttca cattggtata cacatatcta 258060gctccttcga aacagacaca cagcatgcta ttgtaagggt gtaccctgct tttgttatca 258120gtcagctctc aatggacttt tgggctatat acagttttta attcaagcag agctgctggg 258180tccatcctta aatatttatg acttatttat ttatttattt agagacaaaa gtctggctct 258240gtcacccaag ctggagtgca gtggcacaat ctcagttcac tgcaacctcc gccccccaag 258300ctcaagcaat tatcacacct cagcctccgg agtaggtggg actacagccg tgtgccactg 258360cacctggcta atttttgtat tttcgataga gatggggttt gccatgctgg ccaggctggt 258420cttaaattcc tggcttcaag tgatccacct gcctcagtct cccaaggtgc tgggattaca 258480ggcgtgagcc accaacctgg ccaaaatatt tatctttatg cacttgtctt atatccttag 258540gaggagtaca agttcagcac ttcaaacagt gctggaacat agtggatcct caattaatat 258600ttgctaaata catgaagtca aattattgct ggaagagaga gtattcatgt ttaaaattct 258660gatatacaga caaaggatcc ccagggctcc cctggcaaga ggatgagggt ctcattttcc 258720aataccctta ccaacactgg acatacacat tttgtcatgg ttgataagtg aaaatggcct 258780ccttttgttt taatacattg tgcttcagaa aattaggaga tgtttttaaa aagtcaacat 258840aaatagttct cattttttat ttatttactt aagccttgct gctgctcaca aaggaaagta 258900gttcagaagc acggcttata tgcaaagctc tgtggttcaa agttaagtca gtctgaatta 258960ttttgacttg attacctgaa gctgttcagc aacactctgc ccactgattt cattcaaaga 259020ctgttgtaaa gatgtcttac tcttaatgag ctgatgatat aatctcttta tttcattctg 259080taagataaat gtcggaaact gagcaatctc atttaggcct tcattatctt tttttatatt 259140tagttggttt ttataatttt tgtgacacta gaaatttcaa acaaatacaa aaatagagaa 259200atccccattt tatcatcatt aaacttcaac aattattgat tcatggctga ccttcttccc 259260tctacatcct ccctccagct ctcaactgta cctaatctcc tttccttcag gctattttga 259320agcaaatccc aggtctcaag tcatttcatc agtatacgtt tcagtatata tttctaaaag 259380ataaagatcc ttctctttac tccaacatac tcctcccctg ccatcaataa taattcctta 259440atatgattta actatccaga cagaattcac atatccctaa ttgcctataa tttttctttt 259500tgatttgttt caatgatgat ccaaatgagg tccacatgtc atgatggata aatacatctt 259560agatctctct taacctgcag aatccctgac cacttttttt gtttcttgaa tgtgtgtatg 259620tgaaaaaatg ggtcatttgt tcagtacagt ttcccacagg ctggatttgg cagactgcac 259680tcccagtgtg ctgtttaacc tgttcttctt ttcttgtgtc tgttttactt ggattctagc 259740agcttggtca gattcaggct tgatttttat gggcaagcct cattcataaa tagtattggg 259800tttcatttct ttttcattag ataattttag tagcttccta actcttctac ctgcctggag 259860tctctgcctc tccaattcac tttagacaac tgctggagga atctacctga aacatacacg 259920gattgtcatt ccttagctca aaaaccttaa tggctccctc tagctagaaa aacaaaaaca 259980aatgcctccc ccgccccacc attttaattg ttaaatggct aaatgtaata caagaaatga 260040aacattattt tagctcatcc taacaaattc ttactcaaga ctctactttt tttatttttt 260100atttttttac ttttttcttt ttttgagatg ggatctatgt tgcccaggct ggtcttgaac 260160tcctgggctc aagcaatcca cctacctagg cctcccaaag tgctgggatt acaggcatga 260220gctactacgc tcggtcaaga ctttaaaggg cttcaccttt ttttttttga gatggagtct 260280tgctctgccg ctcaggctgg agtgcagtcg tgtgatctca gctcactgca acttctgcct 260340cccaggttca agcaattctc ctgcctcagc ctcccaggta gctgggatta caggcacaca 260400ccaccatgtc cggctaattt ttgtattttt agtagagatg gggtttcacc atgttggcca 260460ggatggtctc aatctcctga cctcaggtga tccgcctgcc ttggcctctc ccaaagtgct 260520aggattacag gcgtgagcca ctgcactcgg ccagggcttc actttcaaat gatctccaac 260580catgagcaac aaacagaaga gtctcatcat ttcccttagc aattcttatt aacaaatgtc 260640accaggaata ggaataggca ttaggggttc atgctgtaac ttaaaacagt gccaaaatag 260700gctgtgtgct cggaatcata cctggtaact gcggttgtaa tcgaggccag ctttcaggga 260760cttgcttcta cagacagctg cagcctgagt gtgctccagg cagacttgaa ggttgtcaaa 260820acattcaagg agcaagttgg tgttctcgcc aggccaagtc atggctttca aaagatcacc 260880cttcttgtca cttagcgcta aataaagttt cttcaactca agagccagcg tctggagtgg 260940agaggaagga agacaatgaa tgagctctga cctcaagagg cttccacgtg ggcttaactc 261000acggggctgc tgtaatggcc aacacatggg ctcgtgagaa gtgccctacc tcgtagtgca 261060cctgctggcc agaaccatcc tccctgtaaa gtctgggcat ctccagcatc tcctccacac 261120tgctgaggca ctgactgagg gtcaggaata gttcaaacaa ttttttatcc aaattgatat 261180atgcttcttc tgtcatattt tcctgaatca agaggaaaaa agaaaaagaa aattaagata 261240cctttgcctt cctagaccta tattttgcct agtttccatt atagtttatg atttatgtta 261300tgcttctttt gtaataagtt gcttcaaacg ctttgtgaac taggtagcag aacaaataaa 261360taaacttacg atgttaaact tggagaaaaa tgagtttaag aagctcaacg ttaggtagtt 261420atttgagtac tgcatggaac taaaaggtaa atcatgagat gaaggatccc ttagcagggc 261480gatccaggtg aaagtgtggt cccagataaa ttacactgtc tcctttacca ctgtttccca 261540ccctccacat tctaatgtag gagaaagcgg ttaatgaaat caagtatagc tgggagaagg 261600aaaagaagtc acaaaagaga agcttgaagg ggttgattac aaagaggagg tacagtgtct 261660taaaaacagg agcaggctgc agaggagggg gaaagtgtca tttatgagcc gattgtgaga 261720atcaaggctt aggtgtatga ctccaagcac gtccccagtg tcatgcattg cgcctggtga 261780gtctgggagc cacagatctg ttttagcata agctgctggg gccacctctt tttctttacc 261840cacagttacc ctgaattctt gaacagttat aagtagttta ggagcatgcc atagctagtc 261900atcactccaa cttgggccct gtctcctact atcttctctg atagacacct cacataacct 261960agcattaatt acctcagtat taagtctgct cagggttgaa aaccatgcct atactccctc 262020cctactctcc tcttcaccct tccaatccca gatcaccact gcagacggca ctcatcaatc 262080atgatcctct ttcctgctga tcctggactc agaatccttt gcacacagtc cctagggaac 262140cactacaaaa tatattagag ttggcaagca agatcaattc aatttgcaat cctaggaccc 262200attccattat gtttcctttt acttgttcag tgacgacaat tatgaccaag tcacaatgat 262260gatatctcct ttgtttatgt tattacaatg aggaaaacac attgtgtgca gcttctacct 262320gtgtctgaag gttacttgct tcttggcgca ggtcttcaag ttgggtggta taggttttca 262380gttcttcagt tgttgggtat ctaaagttat acatagtcac gctgggtaga atacccatat 262440ttgtggaaac ctatttgaca aaggaaaacc ctattactga cagttttaga ccctgctgat 262500gttaattaat ttattttttt attttcgaga tggagtcttg ctctgtcgcc caggctggag 262560tgcagtggca tgatctcctc tcactgaaac ctctgtctcc catgttcaag agattctcct 262620acctcagcct cccgagtagc tgggataaca ggtgcctgcc accatgcccg gctaattttt 262680gtatttttag tagcgacggg gctttgccat gttagctagg ctattcttga actcgtgacc 262740tcaggtgatc cacctgcctc agcctcccaa agtgctggga ttacaggcat gagccaacat 262800gtccagcctg ctgatattaa tttaaataga aaaagatgcc ttagcatgct gtcttctgag 262860ccactttctt ggtgaacatt tttgtctatc ttgccattta taagaaaaaa caattaagtt 262920ttaatatttt ggacagcttt gaaaagtcat gactagttat atttagatac ttgaggtttc 262980atttagatat caaaatgaga ggaaaggctg ggcttagtgg cttatgccta taatcctagt 263040actttgcaag tagaggtggg aggattccta gaggccagga gtgcaaggga gttcaagacc 263100agcctgtaca acataacaag atacattctc tgcaaaaaaa aaaaaaaaaa attatacaaa 263160aatttttaaa gtagctggtc atggtgctgc acacctgtag tcctagctac tcggaaggct 263220gaggcaggag atcgtttgag cccaggagtt caaagctata atgagttttg gtcatgccac 263280tgcactccac cctgggtaac agcgagaacc cgtctctttt tttgagacgg agtctcgctg 263340ttgtcaccag ggctggagtg caatggcaca atctcagctc actgcaacct ctgccacctg 263400ggttccagca attttcctgc ctcagcctcc caagtagctg agattacagg tgcccaccac 263460cacggccagc taattttcgt atttttagta gagatggggt ttcaccatgt tgactaggct 263520ggtcttgaac tcctgacctc aggtgatcca cacgcctcag gctcctgaag tgctgggatt 263580ataggcgtga gccactgcac ctagctgaga ccctgtctct taaaaaaaaa aaaaaaaggt 263640ggtgtgtgtg tgtgtgtgtg aaatcattaa tctttcagtt tagtaataac aacattcacg 263700ctacagggct agctgcttat ttaccagttt acttttgaca cagccaacta ttagaataga 263760attcaagtct cttctaaggg atgggtttgc atcttgattt gttattgaaa agctaaataa 263820gtactgaaaa tgagctgtga tttcagaagg gtgaatgatt cagaattagc tcctttctca 263880aactggaaag ctgacaaata aaatgaaaca gggtgaggtg gtagatgact ctgcttgggg 263940aaagaactaa taatgagtgg agttctcctg tttgcaagag acctatgtac acgttacagt 264000gtaatatgga gaaaccaagg ttaagggcca actgatgagt ttgattgaac tttaattggt 264060caaataaggt ccttagcggg aaagaggaca gctgagaagt cctatcagac tgggaaattg 264120ggaaatcacg ggtaaccttt gcccaggcaa tgtgagtatg tggtggaagg cagaagcctg 264180ccttgtgcgc agtggggctg ttttgctcac ctgctccctg tctggagaga gacagtagtg 264240actgacacag caagccagcc tgatagagcc tgtgacggca gtgataagga gggccaatca 264300cagggaacac cgattggcca gtgccatcgg cacaggcgtt ggcacatggt agattccaaa 264360gaattttctg atgaatgaat gacactcatt gtagcgctgt atagttttta aagtgttttt 264420tttttttttg agacgcagtt tcactctcgt tgccctggct ggagtgcaat ggcgcgatct 264480cagctcacca taacctccgc ctcccaggtt caagcaattc tcctgcctca gcctcctgag 264540tagctgggat tacaggcatg caccaccacg gccagctaat tttgtatttt tagtagagac 264600agggtttctc catgttgagg ctggtcttga actcctgacc tcaggtgatc cacctgcctc 264660agcctcccaa agtgctggga ttacaggcgt gagccaccgc gcctggccaa gtgtttctta 264720aaatacatat tcattccttt attcattcaa caaacagtaa ctgagagtct gtgatgtcct 264780aggtacggtg cttggcactc aaatgcaaac caggcagaca tgatctgtct ttcttcccca 264840tctaatgggg aatacagact ataaacaaac aggcggatag ttgttataaa taacagctct 264900gatgagggaa gccctgggtg ctacagaagc acaaaggaag ggttcctaat gcaggtttcc 264960aagatcaaga aaatgtatca gccaagtaaa aaactcactt ggcaactttg ctttctggag 265020aatatacatg tttatttaat gaatgcttgt tggattttca aagataaaca ggctatttgc 265080accatgtaga tacagactga cctgaggctc cacaagctga gggagtggga gctttatttt 265140tgatgagagt tcatgatgca gatattgcca cttatctcca ttttggccct ggggtgacaa 265200gatcgagtct ggaacgtcat tttcagggct ggatgcctgg ttgcttttga agaagaacaa 265260aaggtgaaga gaataatcaa cactaaacat tttgtattac attcacagtt ctatgagcaa 265320tttttaaaag aaacactctt aattctccca gtcataaaac cactgaatga ccaactctat 265380ttttaaatgt tttaccttgc agatgattcc tgagttgtat cgttatcatg ttggcaatac 265440tggggccaca ttttcttagc attaaattct atgaatttga tgaaatcttt ctgttccatt 265500ggttttaact ccagaacctg taattgaatg agattcaaat ggtccaatcc atcaagcttt 265560tcgattagta cccactagtt tcctctgctg agataaatct aattttgagc cagttcctaa 265620atcattaatt actatttctc tttcttgcat tcctgttctt gtaaactctc acttcccaaa 265680aacctattta aaatattcac acaataactg ttatttaaac cgcatacatg gaaaatcatc 265740aaagacatac aaacacattt tgaaaagaca ggcaaagcag agtcgcaact acagaagttc 265800accctgaaga cagctgatat aagatgtaag ttaataaagg agcaaagtta tggtgtcccc 265860agtgttagca gctgaaagca aagcagatgc tatggtaaat gctgaaaact ctcagagttt 265920ctgcacacac acaaacaccc acatgccaca ctttgcagaa attccatata gaaagctttt 265980ttttgtttgt ttgtttttgt ttttggagac atggtctcac gctatcaccc caggttagag 266040tagagtggtg tgatcttagc tcgctgcaac ctctgcctcc tggattcaag tgattcttgt 266100gcctcagcct cctgagtagc tgggattaca ggcttgcacc accatgcctg gctcattttt 266160ttgtattttt agtagagcta gggtttcacc atgttgtcca ggctggtttc gaactcctga 266220cctcaaatga tctgcctgcc tcagcctccc aaagtgctgg gattacaggc atgagctact 266280gtgcctggcc ttttttttgg tttttgaaac agggtctcgc tatgttgccc aggctggcct 266340cgaacacctg gccacaggtg atcctcccac ctcagcctcc caagtagcta ggactgtagg 266400tttgcaccac cttgcctggt taaaaagtta tattctagtc cctacaactg ttgggggcta 266460gaattacctg ttgctgctgg aaatcttttt gtctgctgaa ttgtggcctt tcagttacaa 266520tggattccaa ttcagaaagg ttaactggtt ggagagcttc ttgatgctct ggctctgagg 266580atttctttag aatggtagga tgctgcaaaa catttcataa tggcaggcta tttattttta 266640aaaataaact ccactttatc tcaattctaa taatgggact acatagaaaa attttacatg 266700aaataaatga tggtattttg ttatttgcta taattatata ctataatttt ttgtttttgt 266760tttttgagat ggagtttcgc acttgttgcc caggctggag tgcaagggca tgatcttggc 266820tcactgcaac ctccacctct tgggttcaag ggattctcct gcctcagccc cccaagtagc 266880tgtgattgca gttgtgccca ccacacccag ctaatttttg tatttttagt ggagacgggg 266940tttcaccatg ttggccagac tggtctcaaa ctcctgacct caggtgatct gcctgcctca 267000gcctcccaaa gggctgggat tacaggcgtg agccaccaca cccagcctac tatgatttaa 267060tgtacctatt tcataacaac ttcaaaaact actacatttg gtgaagtgat catcttactt 267120cttcaagcta attcatccaa tgaattacta aaagcaagaa aatgtattgc cttttttctt 267180aaaaacaaat accaaataaa aaagtaccaa aaagagaaac agtattataa attcaaattt 267240cccctatggc taaaatacat tttttttaaa aagagagaac atattaggat gaaaggggaa 267300ccatatggcc tatgtatctg cagccagtct tatttctgtg aacataaatc tctaagtagg 267360aaccactcat aagcacatgc aaatctttta tagtaagatc tactcataag atgcacattt 267420gcatttataa acacagagtg cttttaaaaa tcatgagaac actttatatg atactttgat 267480tatatatata aatacttgca aatgaataac aaagtatcct ggaaacaggt ttatgtttct 267540gatgtcaaat tttagtcaca tctatgaaag ctttgttttc cagtcaatta gagtcacaaa 267600attcctttat aatacatcct tcttttacga agaagcctta cactagacct cactgccaac 267660aagaacactc agctacatta aaccaacaca aaccaaacca ccgccatcac caacaacaac 267720ctagacagga aaactgcaaa gtagaatgaa ataaaaataa caagccagtg tactcatcta 267780tggtgttaat gtgtgaaatt ctttcacact tagtacagtt tctctgtagc tggttcatca 267840cttccaacca gagttctctt ctggcaggtc tgccacatat gtgtatcaag atgaatgtcc 267900atagatagtc attttttacc tgatcttcac tgtgcttctc ctgaagcatc atctggactt 267960tttctaaatt gcacttcact gttttcagtt tcagggaaag cgcttcagcc tcatgttgag 268020tggctccatt atctcccagg ccctgatctt tgcaagtctc taacagaaag gcaaccttgt 268080gctcaatctc tgttagcata gcctaggaaa taaaagcatt taattattca taatgttgtt 268140cttgaagaag tattgtgcct gcaaacaagt taagacttac tctttttact taagtgtata 268200caagaagtcc cagaaacaca ggatggaaga aaccctggaa aaacaaaaac ttccacagaa 268260ttcttgtact tttcagtagg gatgcaacca aaccaatccc aattacacat gtcactctgt 268320aaaataattt aagtaaatga ctctatagtc gccttacaaa acatagcaga atgtatccag 268380cagaggatag tggttaagaa cacagatctt ggaactagac tatctggttt cagatcccag 268440tcctaccact tagtagatgt gtaaacacag gcaccttact ttagaggagt gtgcctctag 268500tagtctgtaa aatgaagata ataaccgaac tcacctccta aggtcattat caggagtgaa 268560tgaattaaat ccatgtactt tggccagtgc ttgatacatc tgtgcttaaa aagtgcagat 268620attcttattt tccccctacc ttcctaatct tttttttttt ttttttctga gacggagtct 268680cactctgttg cccaggatag agtgcagtgg tgtgatctcg gctcactgca acctccacct 268740cccaggttca agtaattctc ctgcctcagc ctcccgagta gctgggatta caagagtgcg 268800ccaccacgcc cagctaattt ttgtattttt agtagagatg gcgtttcgcc attttggcca 268860ggctggtctc aaactcctga cctcgtgatc tgcccaccta ggcctcccaa agtgctggga 268920ttacaggcat gaaccaccat gcctcgccta ccttcctaat cttttatgtt gcaatccaag 268980tgtggagtag gaatttaaaa aagaattcca cgtaactgtt tgaaaattat ttcccttgct 269040cttttccttc tctttctcaa gacaaataag cctccaaact ctcacaacac catagaagaa 269100ataatttgga acctgaaata tttagttatt gatacaaccg gccaattcac acaaatctaa 269160aaatatatca gctggatgaa gtaagtttcc cagtaagtct cggtcattgt attcctgctc 269220aacagatcaa gtttgccctc tcctggggct cagcagcctg ctcggtcatg ccagctggct 269280gggcactcac gtcagcagca acagacaaac tcacattcca gcaccatgat catttgtact 269340aaaactcggt gcgtgactaa gaacaagttc cattcattga gcgaatttaa ggaattaaaa 269400attttcctta aggacgaact ctattcttaa aacaaaattg caaataattt atcctataac 269460tccttcatcc aagcaagctg taacaggccc ctttatctat gcttttactt tctgtggttt 269520cagttacctg cagtcaatca gggctcaaaa atattaaatg gaaaattcca gaaataaata 269580attcatacat tttaaatttt gcaccacctg agtagcgtga taaaattgca caccatcctg 269640ctccatcccg cccaggatgt gaatcatccc tttgtccagc atattcatac tccagcactc 269700ggctcattag ttacttagta gaggttgagt atacctaatc tgaaaatcca aaatcccaaa 269760atgctccaaa acctgaaact ttttatgcac catgccagaa gtggaaaatt ccacacccaa 269820cctcatgtga tgggtcacag ttgaactctg tttcatgtat aaaattatta aagttattat 269880ataaaataac cttcaggata tgtatatgag gtatagataa aacataaatt gaggcctagc 269940atggtggctc atacctgtaa tcccagcact tcgggaggct gaggcaggag gctcacttga 270000ggccaggagt ttgagaccag cctgggcaac acagtgagac cctgagtcta caaaaaataa 270060aattagccag gcatggtggt gtacgcctgt agtcccagct attccggaga ctggggcagg 270120aggattgcct gagcccgtca ggtcaaggct gcagtgagcc atgatcatgc tactgcactc 270180cagcctgggt aacagagcaa gaccctgtta actctaatat aaataaagaa attaattaat 270240taatttagtg tttagacttg ggtcctctct ctaagatatc tcattatgta tttgcaaata 270300ttccaaaatc cctcccaaaa aaccaaatgc aaaatacttc tagttccaag catttgggga 270360taagggatac tcactctgta gccatctcag ttgtcagatc gaaaaaacat aatacacaca 270420gagtttggta ctatccacgg tttcaggcat ccattggggg tcttgtaaca caacccccac 270480agacaaaggt gggactactc tacttatgtc ctctatagat tcttctaagt ttaaacaata 270540tgtacccata gactgtacag tggctataaa caaaatgtag cataatttta tgctctgttt 270600ttcccactta actattacat actttttcat ctgtctccac acgtttcatg attttcattt 270660taacagctac aagttgatat cccatgattc acttaccaat ttccctcttg ttaccccttt 270720gtttctagtt cttcactttt gtaatcaatg cttctataat tatttttctc atttgggatt 270780attttcagaa ggtaaattaa aagtaatggt actggccggg cacggtggct catgcctgta 270840atctcggcac tttgggaggc caaggcaggt ggatcacctg aggtcaggag ttcgagatca 270900ccctggccaa catggggaaa ccgtttctac taaaaataca aaaattagcc agatgctgtg 270960gtgcatgcct gtaatcccag ctacttggga ggctgaggca ggagaatcac ttgaacccag 271020gaggcagaag ttgcagtgtg ccgagactgt gccactgcac tccagcctgg gtgagagcga 271080gacttcacct caaaaaaaaa gtaatggtac ttttaactca aagaagatga acagcttata 271140tgttgacaac tgacctccca aaacattgag ccaattagat agaactacca gacattagag 271200tatctatttt tccagaatga tattttttta atgtaatgtg acagctgttt taatttatgt 271260ctttaataat caaaattaaa caattctcaa atattacatt actaagcata atcctccctc 271320cctccctccc tcccttcttt cctttctctt tctttttttt gacagtgtct tactctcttg 271380cccaggctgg aatgcagtgg tgcaatcata gctcactgca gccttgaatt cttgggctta 271440agagatatta gtgcctcagc ctcccaggta gctgggacta cagatacaca ccaccatgcc 271500cagctagttt attttttaat ttttttttaa attattttgt agagacaagg tgtaactatg 271560ttgcccaggc tggtctcaaa ctcctggcct caagcaatcc acccgccttg gcctcccaaa 271620gtgctgggat tacaggcgtg agccaccact cctggtccat aatcttcact gaactgtttg 271680ctcctgtcct ttggccattt ttccactgga attttgtgtt tttacagacc actttaaggt 271740ttttatatgt ttcaataata actttattgt gttatgttat ataaatgagc caaagatgcc 271800tttgtatgtt ggccccatgt ggtttttctt caaagcaggt cagaagcagt aagttcaaaa 271860agtgccagca ccagactcaa atttttacac atttgacggc tttaaatgca gcccaaataa 271920gcatatttta atccatttag agcctgtctg ctttgcatac ccctgaaaac tgcacccaac 271980atctgccagc cacggataag actaaccctg ggctataaaa aaatcccaag cctctgctgc 272040ccttcggcgc tctctgaccc agagaccctc cactgtgctg agacatcgct agacacataa 272100gttccctctc cgtttctcct ctcccctggg agttcccttg ccttcctccc cctctgggtg 272160gtggcctgac ccccagtcta tgccttagcc tctggaaggt ctcctgcagt aaagagcttc 272220cctgtctctc acgatcataa tcttgccaaa atgctgccca aataaagctt gttgggtgca 272280actgccacct ggtagtcatg tcttttcctt gagcagcctg gaaatccttg aactcactac 272340aagtggcaat gaagatggga ttctggtgat aatcaacgag ttggcatagg gtctacccag 272400tcagtagata atcagttggc aaatcaatcc tgaatggcct taagtgggtg ggactcaaaa 272460tctggactgc ctcgagttga gtttagcata gcattgctct gtgctgtatg acgctctatg 272520gcattcacag gctcccctgg gcctcatcag ccaggccaag cggcagtgcc agtatctgag 272580tgaacctatc cctttagaac tgacttttgg gctgggcatg atagctcacg cctgtaatcc 272640agcactttgg gaggctgact tgggtggatc gcttgagccc aggagttcga gaccagcctg 272700ggcaatatag tgagacctct catctctaca aaaaaataca aaaagtagcc gggtgtggtg 272760gtgcagggct gtagtcccag ctacccagga agctgaggtg ggaggatcac ctgagcccag 272820gaagtcgagg ctgcaatgag ctatgattgc tccactgcac tccagcttgg gtgacagggt 272880gagaccccga aaaaaaaaaa aaagaactga cttttgagat cagaggtgcc gagatgcaca 272940gaaaaggagg ctggcctgtc tgtctggttc ctggatgttg ttaagactat gggcttacat 273000agaaggggag aatgcacaac tgtggatcaa aggcaggctt cccacaacaa cataacaggc 273060tacagcagga aacgctccct gtggcagggc tagtgatatg cgaactctga gacaagagga 273120tgtcttcatc ctggcagaca agcccatatg ataaagacaa gtctggtctc ctaagtgcag 273180ctgggagaga ggcctttgca agcagctcgc gtgcccctgc tcttgtgaca ctcgattatg 273240ctgttgcaat gtcatcattc tagcctctcc ctggagggat tagaaacccc aaccttcggg 273300ctgagccggc aagctttact cagagcgact agcggcaagg aagtttatat ccctgcccct 273360cccaagcaaa acccagggca ttcctagtaa aatggttgca aagccctttt atgctgtatc 273420ttcaattttg caaattaata cttttggttt catttatgat gaatgaatat tctaattact 273480tactaaaatt gaataaaata atagcattta ctgagcactt gttatatact ttatatacct 273540atttaatcct gacaaaaatc ctatgagata actttatatc ctatgacact gctttaaata 273600ccttatttaa atcctgcgac actactttaa ataccttatt taatcctgac aaaaatccta 273660taagataacc tactatataa ggaaaatgag gctgagaagt tcacttcccg aagttcacag 273720ctaatgtgac agagctggat ttagactcct agattctgaa cactactgtc ccctcaaata 273780agatagttcc ataaagcatt tcaaagaatt accctgagaa ctaaaagctg aggccaagat 273840aaatgtaagg gtcagagtaa cagaaagaaa aacagtgttg ctttaagaga aattcaccca 273900atcttggcag atgattggtg gagaggcaaa ccatgaactc tgacttctca gtcttacctg 273960gcaccctacc agctgctgtt ccagcacctg ctgcatgtct gcgtttaatg tttccggctc 274020aactgccacg ttggcttgtt gcagccacag ctccagctcg gccacctggg tcttgcaggc 274080atgcaggact tctgtgggct ccggcctagt tttctccacc ctgggctcca aacctccttg 274140cgcgtcagaa gagtccagag tgtcagcctc aataggaggt gtggtgccct aagagaaatc 274200caaagataag ccagggagtc caaagttttc agacttgggc ctgcaaagac ctgggaacct 274260gcaacatttc cccagttaaa agaaacaaaa tcaggacaga agaaggaaga aagaaaagag 274320aaatgcacct ccacactcat agcaacaatc acgtgacctg gtgccacagg tttccaagca 274380gccgatctgc gagtaagcag gtacagtatt cccgggcaca gaggacgcaa acaggctcac 274440agttcactac ccggtgagcc ccaggagaca ggtgagaatg tgagtgacag ggtgtgtgtg 274500tgtccgaaac atttaaggga attggccagt gtttagcgtt acgtgacaca tgattaagca 274560atgcttacat tgctgcacac tctattccaa cagaaaaaca aaacaaaaca gaacacgatc 274620tttacctccc agcagggata caataaaata taccacataa ttttaacttg acatcttctc 274680aaatagttag attataaaaa ttttagcttt ttaaaaacat acatggtatt acttatctaa 274740atttttccct aataaaaaca gcttgagtca agtaatgaac ttcagtgggg taacctggaa 274800acttttttcc tattcctcag aaggaatccc taaaatacct tccttcccct tctgcatact 274860cgaatatgat tgagttaagg actagatgct cacagtataa agaaagtgtg gtgtgttctt 274920tttacattga aataactggg ttgtttatca actgattgga aaattttctc ccagatacca 274980aatgaaagta ttccagtaat tatatattag atatgttcaa cctggaggca aaagataatt 275040ggatagagag atgtatttac ctcccactgt attatctatc cttcctcaga atatggaaaa 275100agtagtatcg atacttctag agaaattcct ttaggaataa ggatttgtgt tttgctgtat 275160aaaccttttg ctctcaaaaa atattttatt gattatgata aaatacaatg ttttctataa 275220gagtttgtta acgtgttagc atctaataat aaaatgtaat tctagccaag tgacaaactt 275280tgaatgggaa tcgattgcaa atatataccc taactcaaat gcacattctt caatgtttac 275340ggaattttgt ttagacatag aactttcagt gtaaaagagc gaacaaaatt ggtaggtggt 275400aacaaacacg tgtcaggcat gagaatgctg ttcattttca tgcacactga tgagtattat 275460gaaagctggt ctggcacaag tgtcttgggt tgtacttttt acctttcacc atctattatc 275520ttcacataca tgtgctgttt tctgccaaaa tgccatttct tccctgcctt cattaatcta 275580ttattataat tttcttcaag aatcttgaaa accttctcaa ggaaactttt ccttgtgttg 275640tgctttttct gaacttcata tatagtcaag tctatgcttg gattcaactg ttttaatttt 275700cttttttaat cctgaccttt ttttttcttc ttgggaagag tttccctctg tcacccaggc 275760tggagtacag tggcatgatc tcggctcact gcaacctccg cctcccaggt tcaagtgatt 275820ctcctgtctt ggcctcccaa ggaactggga ttacaagcac acaccaccat acctggctaa 275880ttttgtattt ttagtagagg cagggttttg tcatgttggc caggctggtc ttgaactcct 275940ggcctcaagt gatccacccg cctagacctc ccaaagtgct gggattacag gcataagcca 276000cagcgcctgg ccctgatttt tttttaaaaa aagcaacagt tgtgtgattt tgcttccaga 276060cttccagata tcaatttcta gtcatctgat taattccctt ctagttttca gatattgcta 276120tatctagttt tcagatattg ctacagacaa agattttgga attccctttc ctttttttgc 276180cacttgagag aacaatctat taatattaat taatctaaga caatctatta atattaatct 276240gtaatcacag atttaccacc tgccttcatt atttattcaa aaattttatt tgagcacctg 276300ttatatgcca cgcacatttt tttttttttg agatggggtc ttgccatgtt gcccaagtgg 276360gtctctaact tctgggctca ggtgatcctc ctgcctcggc ctctcaaagt gctgggatta 276420caggcatgag ccaccacacc cagccctcca cacacttttt ttaatgacag tattgagttt 276480ctctgctttc tcatgaactc accaaattaa tgaggaaata agcaagctgc atgactaatc 276540tgggtttttt ttgctagtta tttgaacctg aaaagaatcc aaaggggaag agaagacaaa 276600aagaatattt ccaaccatac agcttcaaga gctgagggtg gcctgaggaa cccactagag 276660atcagttcac ccacctcttt ttaaagtcta agaaatccaa gtgcacagaa tgttcaatca 276720gcccacatgc ctcccttttt accatgttgc ttctgagaag cacccaagtt actaacccaa 276780ggtcacacag ctctgtgatc ttttaattca gagttccctt gttttttaca cagtagcatc 276840cctgtaaaat accttctcta tatgatagcc ccaaattcta aaagcaaaac ttatttaaat 276900ggatgtttta taattgtaca ctttggcggg gggggggggg tgtaaaaagt attactataa 276960gtatttctta ttaaataaat atgtcttctc taaagcataa ctgtaaatta ataatagcta 277020gcctttattg ggctcttacc atgagcaggc actgtatggt cacacaacta gtaaacagaa 277080tcaaaagcaa ttcctgcttt cactacactt tggaatctac aagtccacat tcttgatata 277140atttcaccaa ccttttagta tccaagttaa cttttcagtt ggtgaatcac attgcttcac 277200ttctgcatca acacaacact gatctaagta atcagtgaag taatcagtaa tcagtaaaaa 277260aaactgcagt taaaatgcaa gttgattttc tggctattgc tacatttcat gccaaaatag 277320caaaccttta cattttctcc ttaatatttc agtcaattct acaaatacct gcatatctcc 277380cccactgctt atacctgcat atctccccca ctgcttatga aatgtaaaac tgcataccta 277440aagtacagta cctaactgca catgaataaa tgaaaagcat gcccctagaa tggcatggtg 277500gtggtttact ctgaggtcat gcctagcttg atttcacatt ttcacatttt tccatactaa 277560atatttatcc ttggttgtaa tacgggtcaa gaatgactca gaattttact aaaaattatg 277620caattatcat ttggtcaata cacttaacct ctgaaacaaa aggtttgttg aggctgatag 277680aaggctgatg acattggctg cctggtttct gcaaagtccc cactttcctt ctcattagtg 277740atgtgctgtg ccaatgagac agaaatcact aaaaggcagg aagacagtca ccaagaggta 277800tggaacttgg agccagtaaa ggtgtccctg aataatcaga agtggacaaa ttcctcctgt 277860agtaaagggt aacatctatt tttcctgctc caagaattcc tccccagcaa aagttctgag 277920ttaagaagaa aatacaaata cacacataca taaattagtt caaaagagag tcactggagc 277980ctaatagttc aaagctcagg ttttaaactc agagtcagaa agatctgggt ttgaatccta 278040gctttgccac ttactagcta aatgaccttg ggcatcttac ttctctgagc ctcagtttct 278100ccatctgtaa agtggggtaa cagtaatatt tatctcatag agctgttgtt aatgattaaa 278160agagatagta tatgtaaaat cccagtacct ggcccatatt aaatgttcag taagtatttt 278220gttatcctcc tttttcttcc tccttctctt tattgtcatc attatctagt atccacatgg 278280ccagggaaaa gaccctagag gttatccaca ccagcagaaa caatattaag cagaatggct 278340actgtagcta aaacaccatt gaagctacat ctgattttta attagatgtc aaaaataaca 278400ttacggtggg cagatggttt gagaccagga gttcgagacc agcctgggca acatggcaga 278460actgtgtgtc tgaaaaaata caaaaattag ccaggcgtga tggtgtgtgc ctgtggtcct 278520agccatccgg gaggctaagg tgggaggatc acctgagccc aggaggttga ggctccagtg 278580agccatgatt gtggccactg cactccagcc tgggtgaaga gtgagaccat gtccccagaa 278640caaaacccag attaattctg aacagcaaaa aaacatactg actaggtcag caacttcctt 278700agacctaagc attgccactg ggtgtaaacc tctgacattt gtccaccttc ctagacatat 278760attgttaaga gagacagaga atgaaaggga gggaaagaaa gaagagaaag gagtaacaat 278820ttgagaacta agagatattt ttgtaagact agacaaaaga ttgggagaaa aaaacataaa 278880tagaactcag tataaaagaa gaaaagagat gcaataatta aacaataaaa acagcaaaaa 278940catgccaata tcttttatag gagctatttg atgagaccct tggagcttta ggtccagcta 279000aacaccaaaa ttaacttggg aaatagtacc catcccccac accctgctgt aaggccttat 279060tgagaacaac tgaggtacaa gattccaaat taggaagcca gattagaata ttctgggttt 279120gattccttat tcctccattc acaagccata tatatatata tacatttttt taaaagacag 279180agtcttgctc aatcgcccag gcaggctgga gtgcagtggc gcaatcttgg ctcactgaaa 279240tctcctcctc ccaggttcaa gcgattctcc tgtctcagcc tcttgagtag ttgggattac 279300aggcatgtgc caccacgccc ggctaatttt tgtattttta gtagagacgg ggtttcacca 279360gcctgttggt caggctggtc tcaaactcct gacatcaggt gatccacctg cattggcctc 279420ccaaagtgct gtgattatag gcttgagcca ccatgcctgg cccactaact atatttttac 279480tccctgaatt ttgatttctt aatttgtaaa catatggtag gtatgaacct accttataca 279540gtctgagggt taaacagata actataaatg ccttttatgt aacaggctat taatcaatat 279600taacccttct ctactttctt tgcccccata agttctatct acagattaaa gaagtagtca 279660tcaacagaaa gactatttca tgatgacaaa tggtaaatga gtgaaaagcg tctaattctc 279720tatgccatgc ctattttctt tgtaaatatc tgatgacaag tcttccagtt ctacagcttg 279780acagcacata tcttcatatg ttaaagttca caaggaacac ttagcttgac gtacaaggta 279840ttgcaggaca cagaacactg gtcatctcta ggtgctagtc cagctatctg agcagtttac 279900ttattaccaa ccaggactta cctcttctgt ttggttgggc cttagggaac attctgggcc 279960ttgctcagtg tttagtgagt ctggtgtgag gatttgtgcc atggaattat cagaggtgct 280020tattttccca tatgcttcct gtaaagaaaa gaatcagagc tccagaaagg gtcctgccct 280080ggtgccacat gaattcttct ttctgcgcat gctcatttta caaactacct gcttgcaaaa 280140aagtcagata tggagaagtc agatacgatt agaaaaaagt ttgttcatta gggaaaaaat 280200cagcttcccc aagactgtct ttatttgaaa gacttgcatt ctccacactt ctcttcttaa 280260tgagcacata aacaatctag agaattacgt acttaagtaa attcttccag aagtatgctt 280320tgtcttgttg aagtattctt taaaagtatg tccttatgaa aatttaaatt acatggccag 280380gtgtgatgtc tcacacctat aatcccagca ctttggaaga ccaaggcaga agaattgctt 280440gtgctcagga gtttgagacc agcctgagca acatggtaag accctgtctc tatcaaaata 280500attaatcaat taattatatg ataaaggcca catttcaaag agtggggaaa aggtgaattt 280560attccattta ggaacaacta ccaaggcatt ttatgaaaaa tataagtaga attcttgtat 280620tactcctaac aataaattct agttatgaaa aatataacta gaatccttgt atcaccccta 280680acaataaatt ctcattgtat tctcaaagtt atgccataaa aattctaggt gtggggaagc 280740atgacaagaa aggaaaataa tattagtatt gactacataa aaaaataaaa taatatgcac 280800cacaaaagat accataatct aagttaaaga gaaatgacag actgggataa ctattacaca 280860tagtgttaat attttttttt gagacggagt ctcgctctgt cgcccaggct ggagggcagt 280920ggcacgatct cggctcacca caagctccgc ctcctgggtt cacgcccttc tcctgcctca 280980gcctcccgag tagctgggac tacaggcacc cgccaccacg cccagctaat tttttgtatt 281040tttagtagag atggggtttc accgttttag ccaggatgat cttgatctcc tgacctcgtg 281100atccactcgc ctcggcctcc caaagtgctg ggattacagg cgtgagccac catgcccggc 281160caatattctt aatatattaa ttactctata aataataagg aaaacatcaa cccctacata 281220gaaaaacagt caaatggtag gaacaagcaa gtcgcaaaag aagagttaca aaagccaata 281280ggcatctaaa gagatattca acattcttag aacataaatt aatttaaacc actggctaag 281340tttggctaag actgacaata tcaaaaatca gcttgtgtgt tggttggggg ggggggtctg 281400gaaatgcaat tccatgtata cctaggagaa atattgttgg tatagccttt ctttttcttt 281460ctttcttttt tttttttttg agacagggcc tcactctgtc accaggctgt ggtgcagtgg 281520tgcaatctta gcttcactgc aaccttcatc tcccaggctc aagtgatcct cccacctcaa 281580tctctgggac tataggcatg caccaccacg accggctaat tttgtatttt ttacagagac 281640agggtttcac tatgttgccc aggctggtct tgaactcctg agctcaagct gtccgcccac 281700ctcagcctcc caaagtgcta ggattacagg catgagacaa ctgcacctag cctggtataa 281760cctttctaga aggcagtttg aaaatgtgtg tcacatttta aatggatata tactttgatt 281820cagcaatcct tcttttaaga ttaaccaaag ttaagcaatg ggaaaatgac atgtacacaa 281880agaattaaca caacgtgaca gaaagcatgt ggatattata atcaaccaac cttgattgct 281940ctacttgcaa gttgtatgat cttggacaaa ttaacctgaa ctctctgaaa cctcagttac 282000ttcaccttta aaatgggaat aaaacctatc ttacctatgc ttgcaattaa gtgacatata 282060taaggcattt agcatttcct aacaaaatgt tagtgtcttt tcagcttcta ctgaaaacag 282120tgactgttat cagaaaggta taaaccaaga attaaaggta gagattagat aaaattagaa 282180aggtaggcgg ggaccaggaa aatctggaat gtcacgctaa gtttgcattt tcttcagtag 282240ataatcaaac tccatgaaga attttaggct gaggtttcgc atgattggtt ttaggaaaag 282300tcatcagtat tttataagat gtattaggaa aaacaattgg agactgtaaa ccaattagag 282360ggctactcca aaagtccagg gaagtaataa gaattgcttg aaataagacg atggcaaaaa 282420taatgggagg agaggctgta cacagtgaat ggacatcatt ggccactgac aggatgtgac 282480aggtgagaga aatgaggcac ctagtagaac tccaaagttt atctctgaga aaatagtggc 282540accaacagca gacactgaga aattagtaag aggagcaagc tttgaaggaa aggtggtaaa 282600ataggtttga gttggagaaa ctccatccca aaaaagtcta tcacgaggat agaaatgtgg 282660aatagattta tagatttaag agaaaagcca agactagaga tttagtttgg taatcactgg 282720catagaggtg accacaagct ctcagaggaa ataaaattgc cctgaaagaa aacagagagg 282780ccgggcgcag tggctcacac ctgtaatccc agcactttgg gaggccaagg caggtggatc 282840atctgaggtc aggagttcaa gactagcctg gccaacatgg caaaactctg tctctactaa 282900aaacaaaaaa aatgagccag gcatggtggc gggcacctgt aatcccagct gctcgggagg 282960ctgaagcagg agaatcgctt gaacccagga ggcagaggtt gcagtgagct gagatcgcac 283020cactgcactc cagcctgggc gacagatcga gactctgtct caaaaaaaaa aaaaaaaaaa 283080aagagaaaac agggagaagg aagaggacag aacattgggg gaatgcctac catcacagac 283140gaaagagaaa gaggggcaag aaaaggagat ggaggaggga ctagaggtag aagggacagg 283200gaagaacaga attaagaaag ttaaaggaac accgcagtta aagaaggaag aggtggtcac 283260tcccagagag gtcaataagg attaggacta ataaaagggt attgaatttg tactccagaa 283320gtcaatggca gtttaagcat agccagaggc tgaaacaaga ttacaaaagg ggccgaggga 283380gtagaacacc aaggaaagac cttcagtagc aaatgaaaga cgaagtgtag taattgacat 283440gggaagcagg atccagaaat tcagttcatt aaagtaccat actgagctga gtataggata 283500taaacgataa gcttctttcc actcatgaaa tcgtatcagg tgtattcata aaaatgtgct 283560aagtcataat tgaggttcag aatcctattg gaaaacaggg aaagagacag caatttcctt 283620tgcaggatgt caggaggttt cttggagcag gggaagttga gatgggtctt tgtggatggg 283680aaggaagtct acagagaggc atggagaaag ggcatcccag ggaccaaagc cttctgtgga 283740gctactgaag atgcaggctg gttctggaac tatctctctg tttgaagtag tggggccaca 283800aggaagagaa catgttaggg aggggaagcc tgagtactga ggtagagaag gaaggaacct 283860agccagaggc agagactgac aaggcaggta taggcagaga atggcgccaa tgattaagga 283920aagagttggc ctgagagcag ggggaggggt cagccctgga caggaggcag gagtgagagg 283980tttatggatc acgaggatgc agagatcttc cgggagcaaa ggaagcacgt agttttcaca 284040tagccttaca atgattttct aaagtgagac catttgagag actggaaaat atttagaatg 284100tcataaggag taaaaagact tagcgcatct aatttatctt acatactact ttgttccaca 284160aaaaaaaaaa aaaaagatta tggagagacc ctccacccaa aactcccaaa agagtggcat 284220gttaatttac atgctttggt aaaacagtgc agtgaagctt ttagctgctc ctttgaggta 284280tctgtttggt cagtttggat tttcacatat atggagtttt cctcaaaaaa agtactctcc 284340tgttattaag tacacaatag aaatagaacc aagcaggtgc tcattaaggc tgtctttgca 284400tttgtgcaaa ggtaatgtga gaagctttca aagattcacg gtagccaaga tagagaagca 284460accgaagtgt ccatcaactg atgaatggag aaagaacatg tggcatatat acacaacaga 284520gtactagcca gccttcaaaa agaaggaggt tctgtcattt gtgacaatat ggatgaaccg 284580gaagacatta tgttaaatga aataagccag gcacagaatg ataaatactg tatgatctca 284640cttacatgtg taatctaaaa aatatggact gcatagaagc agagaatagg atggtggtta 284700ccagggctgt tgggggctga gagagggcct ggggagatgt tggtcaaagg acacaaaatt 284760tcagttgaac agcaggaata agttcaagag aatctatgta taacatggca attatagtta 284820ataataatgt attgtatact taaaagttgc taagcgacta gattttaggt gttctaatta 284880ctgaaaagat gacaaatatg tgaggtaatg catatgttaa ttagcttgat acagccattt 284940ccaacgtata cacatttcaa aacatgttgt ataccataaa tatatacaat ggttgtcagc 285000taaaaaaaaa aaaaaaaaaa aaaaaaattc agcccatgca ggttccctcc ctaagcattc 285060ctttaaacaa aatagttctt tactatcctt atttttattc ctgtctgagc tacaaatggg 285120cattttaaag gtttaggcag tgcttgctga aaaccaaaca aaggaaagag ggaagagagg 285180aagcaaagct aacatagaaa gtcaggcaac atggcgaaac cccgtctcta ctacaaatac 285240aaaaattagc tgggcgtggt gacgtgtgcc tgtaatccca gctacttggg aggctgaagc 285300aggagaattg cttgaaccta agaggcggag gttgcagtga gctgagatca tacgactgca 285360ctccagtctg ggcgacagag cgagtccgtc tcaggggtgt agggaaagaa agtcgtgcag 285420aaagagatcc ctgctccccc ctgggccctc gtaaccccta agcgtggctg atacgcccag 285480cattacctga acaattgttc cagaggatga ggaagctctg tcttcttcca tgtccttgtc 285540atgcttccaa agtgaagacc aagactgagg cgatggctct gccttctcat ctccattcta 285600tcacagtaaa cgattggtta gcaaagggaa gagcttccct tactgtcata accggtgccc 285660tactcactca cgtcttttat accagactca acttatcagg cagtgtaacc acagcccttt 285720attgcagtcc tcccccattt ttttatctgt atttttttta ctaactatat catgtttaca 285780aatgtggctg ctcttcctaa agcctagaaa atacttttaa aagtaggggc tcggctcacg 285840tcacacaacg gaaatcttta aaatggtgct tttaggaatt taatctgctt tggtaaatgt 285900aaccttaaat ggtatctttg aggccaggca ctgtgactca cacccataaa cccagccctt 285960tgggaggctg aagtgggagg atcacttgag gccaggagtt ggaggttgca gtgagaacca 286020ctgcactcta gcctaggcaa cagagtgaga tcccatctct aaataaatat ttaaaaatac 286080gtaaaagtta gaaaaattaa aatgctatct ttaaaattac agccacgagt ataagaaaaa 286140atgttcaacc tcactaatca ccagggaaat gcaaattaaa accacagtga gatactactt 286200tacacctgtt agaatggctt atataaaaaa gataaaaatg acaagtggtc atgaggatgt 286260ggagacaaca gaacccctga atactgtcgg tgggaatgta aattggtaca gtatcaaagt 286320tcctcaaaaa actaaaaata gaaccagcaa tcccactact gagtacatat ccaaaggaaa 286380aaactcagta tgtcaaagag atgtctgcac tcccatgttc aatgcagcac tgttcacaac 286440aaccaagata cggaattaac ctaagtgtcc accaacaaat gaatggataa agaaaatgtg 286500gttatataca catactgaaa tactatctgg ccttaaaaaa taaggaaatt ctgtcatttg 286560caacaacatg ggtaaattag aggatcttct gttaagtgaa ataatccagg cacagaagga 286620cacataccac atcatctccc tttaatgtgg aatgtaaaaa aaaaaaactc atagaagcag 286680aaaataaaac aatggttacc agcgaccagg gattacaaaa atgtctgtca aaggacatta 286740aaaaaagggc acaggaggaa taagttcaag ggatcttttg tatatcatgg tgactatagt 286800taatatatgt ttgaaaatca tgaagagagt agattttaag ttttctcatt acaaaaaaaa 286860atggtatgtg aggtaacatg tatgttaatt agctcaattt agccattcca caatgcatat 286920atacatgtac tgtatcaaaa catcatacat gtcataaata tatatatata tatatatata 286980tatatatata tatatatttt tttttttttt ttttgagaca gttttgctct tgtcacccag 287040gctggagtgc aacagcgtga tctcggctca ctgcaacctc tgcctcctgg gttcaagcaa 287100ttctcctgcc tcagcctccc aagtagctgg gactacagga acctgccacc acacctggca 287160aattttttga atgcatacaa tttttacttg tcaatttttt taaaaaaatc acagcttgat 287220gttccccttc atgtgtccat gtgttctcat tgttcaattc ccacctatga gtgagaatat 287280gcggtgtttg gttttttgtt cttgcgatag tttactgaga atgatgattt tcaatttcat 287340ccatgtccct acaaaggaca tgaactcatc attttttatg gctgcatagt attccatggt 287400gtatatgtgc cacattttct taatccagtc tatcattgtt ggacatttgg gttggttcca 287460agtctttgct attgtgaata atgccgcagt aaacatatgt gtgcatgtgt ctttatagca 287520gcatgattta tagtcctttg ggtatatacc cagtaatggg atggctgggt caaatggtat 287580ttctagttct agatcatcac actctgggga gtgttgtggg ttagggggag ggggcagggc 287640tagcattggg agatatacct aatgctagat gacgagttag tgggtacggt gcaccagcat 287700ggcacatgta tgcatatgta actaacctgc acattgtgca catgtaccct aaaacttaaa 287760gtataataat aattaaaaaa aagaaaaaaa aatcacagct tgatgaacat gtactttttt 287820gttgctgtca aagacaaaca acactataaa catcagctca gaaggaaata catgtctcaa 287880attaacatcg tactgaagtc actctttttg gcccctggtc cctccattat tttcgtagtt 287940ctctctgcca tgaaggggta taatcagatt gcaaagttgt ttgatggtca cagagcacac 288000agtaaagttg tactcttcct tgtcacagtt ccaccatgtc ccagcgacac actctaggac 288060aagaggtctg ctctgtgtga gagtggagct atttaaccat ctataaaatg agcccaaaaa 288120gtgtctggtg gttacatgta agtcacaaaa attgtaagtt gcttcaattt tcttcctttt 288180gtcttaagac ccattgcaac ttattttttc aagtatgaaa tggaaactga cgtcagaaaa 288240gacaactgaa aatgtctcat tcaaccagtt ataatcaagt agtacaaatt cttagtatta 288300cttgctaaca caactcttca tgtcattgga aatactgttt tagagcaaaa gagtaattga 288360aatgcggaaa aatacagttc taaaaatgag accaggagtg ccaaacagca tgctctcaaa 288420acttcagaga aagtcctcag aaatcaggat actacagaga acttcttcac cttctcccgt 288480atgccaacaa gagtgtctga attacattca tctcactctt acacagtgaa gtaggtttcc 288540atacacttaa acattaataa cataagagaa ctacttcaat catactttta aaatatcaac 288600attaatttta aaaattctta ggtttccatt cactttaaga tcttttcatt gacttctttt 288660agagatctgg ttctaacaaa ctttaacaag aaaatcattt cccttacatc gctcttcacg 288720gaactttctt ccacctcttc ctcgactgct gccaggtaag acatggagcc tcttctgttc 288780aactgaaagg cacagcacaa gaatacctgc agtctgcttg ccagtcaaca tcaccaagat 288840cccagtccag ctgcattttt atgtagctaa ttttttactt actttatcca agatgcccac 288900ccccacaaga agcctgtctc ctggtccgtt cagctcctcc agccagatat ccggagtcag 288960tcaaacctta ggtcttgtta ttttcatggc cccctgtgcc ccttcataaa cgattaaaga 289020atgtgattca ttgatgtgaa tgcagagaag acttaggcac tgggcccttc ttgagtctgc 289080aaagggctga cttttctgta cgaagatact tcaaagggag gcttgatcca cctgcctgtg 289140aaattttata agcatgtcct aaagtaattc aggtgttaag aattctgggt ggagtccaga 289200acaggcacac cctgaggatt tatattcact agtaaacaac ctcaggttga gtatttccac 289260tttaaaaaat tcctttacta caggccatag acatagatgc cacaggtcgt tcaggtggaa 289320aacactaccc attaatgctc ttgtggagtg tggggccagc attaacctaa agtcataccc 289380accttccgct cagaagcatc cctctctgcc actccacctt cctctgatgt tacagctggc 289440agcctatctc tttctactcc ttcttgttct tgcttcaaat gctggtgaag gtttagtacg 289500gtggccttca agtccaccaa caggtcttct tttctctggt tcaaactcag aatctgtttc 289560tccatacgtt cgatttctcc ctgtgtagaa agagtgtttg cataggtttg agaagtctga 289620ggcctctgca ggcccagagc agcctgcttc ctttggtaca agggctgaag atttttcttt 289680gctatttggt ttacaaagcc agaagctagt ctctggtacg gtatagctcc tttccatcct 289740ggatacaatt ccaaaggtcc tgtgtagggt ttataagcta taggatgcct cagccttacc 289800agagaccttg tgaatcagaa tctctgcggg ttgagccaaa gcatggaaac ttgtaacatg 289860ctccacacgt gactgtggtg tgcggccagg gttgggagct accgctctag tggccagcta 289920caggactgag agaaccagga tccacggggc agggccccgg agtctgcaca ctaaccaaca 289980ctgcataagt gccttatgca gactttccat tgtgtcccaa agcatattaa aaatgcatgt 290040ccccaggaga catgttccca tgtctctcca ggaaattctt aagcagacta acatttggga 290100accactgaga gaaaatgaag acagaaatct cattctttta ttatcatctt tgaaggcttc 290160tttggtatta ctaaattcat ttactttttt tttaagcaga gtctatcctg ctcaatcatt 290220tactttttct acagtaaaat cttcctacta aataggaata ggtaaacaaa taaaggtacc 290280atgtagtata tcttattatg ggaatgatgg acagatgatc tcaagcttcg tgatgttact 290340tttaacattt atattattga tatgtgcaga tcaaatttct tattcatggc agatatgcag 290400atgcccactg tatgtctgag atgactatag gattatagat tcttagcttt ggaagaaaca 290460gcaggtgaca aatacactaa catcctcctc tctagcacct ctgagagatg tcattcaact 290520tgcctgagct cctgaagtga gtactgcatc agaagcagcc tgctcctttt tagaaaacct 290580tcatgtgttt gaaatttgtt cctaatatat tccttagacc cctattcttt ccttgttatg 290640tcttcctttc ttgctgtcag ttcatccatc cacccatcct acagatagtc atagaagcaa 290700taattttctt acaaaacaga gaaacgtaat ctgtctccac ctgcaagaga gagttctaaa 290760ggccagagaa agaaggtgat ttgtccaaag ctgcaactag cacacagcag agcacaggcc 290820tgggctttct cctggctgta ctgcacacat ttctatgcca atacccctat tctgtctgaa 290880gtcaaaattt ctgtgattgc ttttgggaaa taacaactgt ttgacttaaa tctgagttgg 290940ctgcattttg tgttccactt tcaataaaca ctaaacttca gaggtacact gcctcctgac 291000aagagcaata ctacagccac taggataaca caaacagagt agaggcacag tcctacatgg 291060agcagctgct ctcaaagcag catctgcaga cccctggcca cagtccatga ggtccagatc 291120attttcataa tactaaaatg ttatttgcct tttacaccat actgacattt gcactgatgg 291180catgaaagca atggtgggta aaactaccgg cacctaatat gaatcaaggc aggaacacca 291240agtatattcg ttgttactgg gttcttcact ttgatgtatt tatagtaaaa aaaattccat 291300tttccttaag aatgtccttg ataaaaatat gtgtcttagt ttatttgtgc tgccgtaaca 291360gaatatctgt gactgggtaa cttataaaga acagaaattt atttctcaca gttctggaga 291420ctgggaagtc caggatcaaa gtattggtac gtttggtgtc tggtgagggc tgttctctgt 291480ttccaagatg gcgcattgaa gactacatct tcctaaaggg agatttgtcg tgttctcaca 291540cggcacaaag cagaagggca aaaatgggtg gactccctcc ctcaagccct tttctgaggg 291600cacctaatcc cattcatgag ggaagagccc tcatgactca atcacctccc aaaggccaca 291660cctcccgata ctgctgtgtt ggtgattaag tttcaacatg aacaaaaatg ttggggggag 291720gggaggcaac attttttggg aggaaaaaac attcaaacca cagcagtatg tatttttagt 291780attctttgtg aaaaaatgga aagtatgcat atggcacttc tgctgcatac caagggccaa 291840tggttgagaa aagcacttat gctactgttt gagttgtaag ctgaactatc ctttttattc 291900acagaacact atttttacgt gaaaaaagcc agctgataac tgtattgctt tccttaaata 291960ctaaaagatt tttctgaaga gataagtgtt aatattaaca actatgattt aaagaatatt 292020agacaatgtg tcaacatttg gaagatcggc ctaactcagc taatcaggat tatccaagtg 292080atcgagcgtg atgtataaaa tcatgcattg ttagaagatc cattcgaagt acaaagtagg 292140ccagtaaatt ttaatgtaaa aacgtataaa gttcattgac atgggtttag attccgtttt 292200acaattaata catactttgc acttgttggg ttttagtata gtttcaaaga aaaatgtcca 292260caattattca aaaggactat taaaatattc ctccatcttc caagtgcatg tctttgagag 292320gctggattgt cttcctatac ttaaaacaaa actacatgct tcagcagatc aaatgcagaa 292380acatttgcag cacccacatc tgtccattag gtgggtgcaa aagtaatcgc ggtttttgtc 292440attactttta atggtaaaaa ctggaattac ttttgcactg acctaatatt aagccagata 292500ttaaagagat tacaaataca taaaacaatg tcactcttct cattactatt tgttttagaa 292560aatataacta ctttaaaaaa atgttacttc tactacagcc tgggtaacac agtgagacct 292620catctctaaa aaaaagaaaa taaaaatagt tattcctatt aatatgtagt gggtttatta 292680ttgttgctta aaaactaaat gaatgttttt acatttctga gttttaattt agttatcaat 292740ggatataatt catataaaca aaagctctct ggggtccttg attttttagc ataaggggaa 292800atctaatatt tttacaatac tgagtcttca ttactgagtg ggaattattg atccaccatt 292860taacagctgt gtcatcttgc actctcctgt acttcactgt agatgtcaaa tcacttgccc 292920caggtctcac agctggcgag tagtgtaatc ttcttttgag taccatatta attgcttgcc 292980tgtatcaatt tgatggcaag aaaaaaaagc agctctctat tacccttagc atacaatcac 293040gtttttttgt tgttgttgtt ttttattata gaaatgctac ttcaaaaaca aagactgaaa 293100aaacctagca acagatggtt aacatgcaaa ccattgtaac tgaatccaca ggacactgtt 293160tttttatttt ttattttttt taattttttt gagacggagt ctcactctgt cacccaggct 293220ggagtgcagt ggcgcgatct cggctcactg caagctctgc ctcccaggtt cactccattc 293280tcctgcctca gcctcccgac tagctgggac tacaggcgcc caccaacaca cccagctaat 293340tttttttgta tttttagtag agacagggtt tcaccgtgtt agccaggatg gtctcgatct 293400cctgaccttg tgatctgccc gcctcggcct cccaaagtgc tgggattaca ggcgtgagcc 293460accgcgcccg gcccacagga cactgttaat tctgacatat ggcaatatgt cagtatggca 293520atactgctta gaatatggaa ttcttcatta caatagtatt cagggtcttc actgctgaag 293580cccagcctgc ctacactgcc tgactctatc ccagaaagtc aggttgtcca aatatcctgt 293640gtgttatatc aggttctttg gtaccagagc aaattcacat caaaatgctt caagctgagg 293700ccatgggcac ttaaaaagcc ctccattaaa gaaggagatg cggcagccct ggctcagttt 293760ctgggattag gactgcccag gctacaaaca gataaggctt ctcttgatca aacatcaggg 293820gctataattt tgtcactttt tagtaatgat aattatataa tcatctattt tatgaaatag 293880ggatggaaag taaacacaca cagaaatacc ttcaattgtt acagagaaac aggtgttaca 293940ctgcaccact agaaaggctc ctagacacca aaggcttagt aagtattaaa taaatgcact 294000gatggacaaa tgaaacatac ctttatgaat gaaagaaaca tacctgtagt tgtggcagct 294060tgtcagcttg gtctggtgac atatgttcaa gggagaactg agctgaataa ttatttaaga 294120tctgtttcaa attttctatt tcttcatccc attcattggt ctgttttatg actacctata 294180aaataacccc cacaaaaaag aatctttgtc ataatctaag cagattaatg gactattttc 294240attgtctagc cccatggaag ttttaatcac tcacagcaaa tatgcaccca ttgagatttc 294300aataggacgt ggtagcctaa acagagagaa gaatgtttgc aaagctgaca cctgatcttc 294360ttgctctact agatgccagg gcaccttggg cagtgcttaa agtacaagtc tcactggaca 294420cacctttgct cttaagtatt tctggtccag tgggtttatt ctggaaagca ttttatggcg 294480tgttaacata aagttgctaa cttttgagaa gttactgaca agtcacatac accattttaa 294540tacatattcc atggccgggg gagcgggggg gctttccgca tagaaaggtg acataacaat 294600tcagactgaa cagagcatgt gaggtcagtg atgatgggtt ttcccggtcc agactcatct 294660tcacctggta ccccttggga tcagtgatgt gtgttttacg agtaagaggc tgagggttca 294720agaacagtct aaatttgcag aataagaact aaccagaaca atacctggca tctagtgcta 294780ggggagcagg aaagctcaga cctatctgtg gactaaataa tatgattaag cacgatgaaa 294840aacaaagaaa cagacttcaa aagaaaaact agatacttct ttggtatgct acataatgaa 294900gcaaaatgcc ttttaaaact acaaagtttc tagccaacac gaaattcttc cttgtatttt 294960ctttaatatt taactataat tttagccata ggctggaaat gaataactat gttcactgtg 295020cacatctgtt ccccagccct caataatgaa ctacatcccg ttctctccac aaaaggaatt 295080tgtcttattt tctacaacga gggtacttac aatatctaaa gtagaaatca aaggaaatgt 295140accatattct aatgattgtc ttcaccactg tcttccttag ctttgaaata tatgagggga 295200catctgattg ccaccagcac aatgagatgg aaaatttgag cagcatagtt tgatacctaa 295260cttggcacaa aacagaactg cctcttctat ttcacccatt tcgtctgtga agaagccact 295320tgaaagcata gttaggaccg tggctttctt tttctgacca ggagactaat aaagcacaaa 295380aggatgaaaa taatggatca aattttatat ttttttctct ttatattatt acaaggtagt 295440acattgagtg ctagatcttt tttctctctg taggtgttac agaatgacat cagttgacca 295500gcagccagac cagagaaact gagttaagac tcaaaccaga gattacagtc tcctcaaacc 295560ttaggagaac agagtggcca ccaaaatgtg catgaaattt aagagggaaa aacatatagt 295620catttttctt cttacaacca gaaggcggtc atgaaaaatg aaaaataacc cttacagtga 295680cccatctaat caggaaaggc agcatgcagg aggttgacgg agacggttgg aagtctgtgc 295740agactttctg caactttctg ttcttgtggg agtgattatt gatccaggaa tacaaactaa 295800agattattcc taactaatga tgattcctga tggagagtag tgatttaaca aaacccaacc 295860accaccacca ccaccacaac acaaaacccc tctggggaga agatgaagaa tatcagctct 295920caatatgcag aaatgacacc aggagtttag aaactcaaat tcttgttcca tgtgtacata 295980aattaacctc tacacaagtt aactctgtgg tctttcagca agtcacttaa ccctgatgcc 296040aatttcctta tctgtaaaaa taaagataaa gtgcctactc tacctacatc acgtttggta 296100atgagaaaat caataggaga gtcactgaga tgaacacaga gattactatg gtgatgatta 296160ctttacagat ctgttggcct cagaggctgc aaatgccttg ttcaggtttg actctcttcc 296220cacagcattt tgcccatgta ggtcaacaac tgtggaatga aattaaatag accataacta 296280tgctttaatg tcttaaagga taagaactga gaaaaataac cttagctcca tttgagcatg 296340aagatatgct tttatatcaa atgccacctc ctacaataat aggataataa tgaatttcaa 296400ttagaagtga aaaccaaacc agcttttggt gaacattttc atccagtaag ttggatgtca 296460tcagaaactg cttaccttta ataactcatt ttgttcttga gtcacatttt ccaatagttt 296520tatatcttgt aaaagctgat ttgtccgctg aaacacaggc agaggtttca ttcctgtttg 296580gggcaacctc agttccactt ggtaagacac tatctcagca atggttttct tcatgggacg 296640tatattttca agtatgacct taattatata agaggagcat gttacagaaa tgaaaagaca 296700atggccactg ttaaatttaa ttattcaaaa ttaaaataca gtatagtttt tatagtttgc 296760atttttaaaa taatttctta gctcagattg aaaagaatta ttatatcact catgatccgg 296820gagttgaaag tctcaggaaa gaataactgg ggcaagagta acctaccaat actaaattac 296880gcatttggac aactttttct tccttttctc cctccacttt ctgtgtagaa ctggcgttct 296940gctgtgtata ttgtcactag gcagtgagca atgtaagggc aggtactgcc tctcactttc 297000gtagcaacta cacctagcac aggacttccc gtatagtagg ccctcaaaag tgcttattga 297060cttgaacaga actgtgctgc atgagtccca ggagtataat tatattttgc tttaataaca 297120tctgatccat agaaatctgg atttatgtaa ccaatacatt agaagagatt actcaccgta 297180ctaagtcata tctcatataa tatcatttca tttaatcctc acaacccctc gagaggtaag 297240tatcctaacc tccatttcac aggtgaggaa attgggctca cagaagttag aagatccagg 297300taagcttagt ctgtaagagg cagagctggg aacagaatcc aggcctccct agtcctaaac 297360cacatgctct caatcactag gctgagcagg ggatcaacat ttgcctgctg tttagtcatg 297420tcccagctcc tctgtcaggg gctctattta cattacaatt ggccaatgta atctcctcag 297480taccagtgca cggatatgaa agagaatcca aaaaccatta attactcagt catgcagcca 297540gtgagcagtc tagctggggc tggaacccag ttcagcctga attaggtgct caagcccttg 297600agtttgattt aaatgaaaaa ataacctata ggaaaattat ctttacatag gtggtactac 297660aactacgatt taaatataca gttatagtta ttcccatcaa tttcccaacc ttattttgct 297720attactctgg taagactaaa tgctttttta aatcacatta tattatattt tgaaaaagac 297780ataataagta tcataagaaa atactttaaa taataatcta tgcttacctc cccatgtttg 297840agatgttctt caggtgaaaa atcaaatatt tcttttgata ataggatagt tttgattttt 297900gaaactcttt tttccattga ttttacttca gattcaatag caaccacatc ctagaatttc 297960agaagaacac actgatgcaa tatatattgt atattaatca tctataaaac agtttattgg 298020cattaatttt attctcttat ttagccaaat aatgtctgcc atctatacct ctgaaaaact 298080aaccaagcac tatagataaa taaataatgt tcaccatttt ctttcctttt tttttttttt 298140tttttttttt tttcctgaga cggagtttcg ctcttgttgc ccaggctgga gtgcaatgtc 298200gcgatctctg ctcactgtaa cctccgcctc ccaggttcaa gcgattctcc tgcctcagcc 298260tcctgaatag ctgggattac aggcacgtgc caccatgccc agttaatttt tatattttta 298320gtagagacgg agtttcgcca tgttggccag gctggtctcc aactcctgac cgcaggtgat 298380ccacccgcct cggcctccca aagtgctggg attacaggtg tgagccatcg ttcctggcca 298440gggaccaaca aaactttata agaaaatgaa gcagtatgta caatagctgt ttatagtgat 298500atcttcaaat tatgaggaaa agttaaatta catggacatt attctgtgca cctactattt 298560aagaaggtat atagaattat acaatgtatt ttagggtaat tttcatcaaa cattatagaa 298620gcatttaaaa attttgaaat gagcttcagt aaccaaaaat aattacttta acacttacta 298680ttccctgaag atacatgagt gtggtgtagg gaatgtaact gagtaacaaa tgtaaataat 298740gaagaacttt aattgcctct actatgtgac tctatcttta aataaatgct tctgatgttt 298800caagccaaaa taaaaatcca gaggctggca aataacaacc cctcaataaa tatttgctat 298860tggtaaataa agacttggga ctacctgttt tacacttgtt tttaattcct ttttgtggag 298920aagggggtct tactgtagta cccaggcagg tctcaaactc ctgtgctcac attatccttc 298980tgcctctgcc tccgtaaatg ctgggattac aggcgtgagc caccgtgctg ggcaccacgc 299040ccagccagaa ctatctcttt taattccctc tttcctatcc agcatttaaa aatcagtagt 299100atttctcttt gacaaagaga aaacactgag cttattttat cgggattctt tactctttaa 299160aacagaccac ttctggattg aaaataagga gatgcttttt gttatagtta cactacaggt 299220tgcaaagtaa agtaaaatat aattccacaa atgagaacat acatgacatt ttaccagatc 299280aatgtgtctt cacattgatt agttgtatag tgcttggtta gtagtataac tctaatttat 299340ttgacagaat cctgctctgt cacccaggct ggagtgcagt ggcgcaatct tggctcactg 299400caacctctgc ctcccaggtt caagcgattc tcctgcctca gccttccaag ttgctgggat 299460tacaggtgtg caccacgatg cccagctaaa ttttgtattt ttagtagaga cagggtttca 299520ccatgttggc caggcttgtc ttgaactcct gacctcaagt gatccacccg ccttggcctc 299580ccaaagtgct ggaattatag ggatgagcca ccattgccca gcctctaatt taatcctgaa 299640tgattaaatg aatggtgcaa acttacatca gccattcgct gaatctgtgg aaggctttcc 299700attatttttt gttgtaaagc tgttacttga ttacttaact cttgtatgga ttgggttaat 299760tcatctgttt caaaaattat tgacaggtct tctagatcca taaagattga atgtaaaaga 299820ttgtgcttct gttctgaatc ttccaaagcc atctagataa acacagaatg ggaaaaatag 299880agagaaacat caagatcctg atataagctg attaatattt aaatcagaga aaattgaaag 299940ttatctcaac attttaaact agtcatttct agttcgataa tgaaaacaga acaccctcta 300000ttgtcaatga cagtgaaaga cttcagagta tctacctgta taactgtaac ataataagaa 300060acataaatat ttggtcttgg tccacttcta aaacacttgg aatcctttaa taatagggga 300120gagagaagtg tcttttatta tacataataa gccctttcat acatgtaata actttcaaat 300180ttaagatgtc aaaatagctc agccacctaa tgaaaatgtt cagcttgata atgtactctt 300240caggtaattc cataaaaaat aactgatagg agaaagaaca ttcacttaca caactagaat 300300ttcagtcaca agtcactaaa actaagtggg ttattcagaa ttttatatta atcttattca 300360aaatccttat aggagaaaac ttttaggaaa acaaaactta aaaccaatgc tttttttttt 300420tttttttttt tttttaccaa ttatcactga caacagcatc aaaaaccata aaatacttag 300480gaataaattt aacagtatac atgcaagaaa tgtatactgg gccgggtgtg gtggctcctg 300540cctataattc cagcactttg ggaggctgag gtgggtggat cacctgaggt caggagttcg 300600agaccagcct ggccaacatg gcaaaaccct gtctctacta aaaatacaaa aattagccag 300660gtgtggtggc ggaagcctgt aatcccagct actcaggagg ctgaggcagg agaatcgctt 300720gaacccagga ggcggaggct gcagtgagcc attattgcac ccctgcactc cagcctgggt 300780gacagagcaa gacaccatct caaaaaaaaa aaaaaaaaaa aaagacctgc atactgaaaa 300840tcacaaaaca ttgttgagaa aaattaaatc cctagagaaa taaagagata taccatgttc 300900ctggattgaa tagtcactat ggttgagatg ttaattctct tccaaattga tctacaaatc 300960taatgcaatc ccactcaaaa ttcccacaga cctttattta gaaattaaca agcagattct 301020aagatttata tcgaaataca aaggccttga tttagccaaa acaaatctga aaaaggacaa 301080agttgtagaa ttgatgctac ctgatttcaa gacttactgt aaagctatag taatgaagac 301140tgtcatattg gtgaaagtat agacatataa attaattgaa cagaagggag agcccagaaa 301200tagatgcaca tgtataaggt caattgattt ccaacaaagt gccgaagtaa tttaatggtg 301260attgttatca ataaatgatg cagaaacaat tggatatccc catggaaaaa aagaactttg 301320atccttcaat cacaccttat gcaacaatta acatgaaatg aatcatagat ctaagagagt 301380aagagttaaa actatcaagt tactggaaga aaacacagga agaaatcttt gtaaccctgg 301440gttaggtaag gatttcttag aatgcaacac aaaagaataa tccataaaag aaaatattga 301500tacattggac ctcatcaaat taataacatt tgctctctga aagacagtgt taaaagaatg 301560aaaagacaag ccgcagactg gaagaaaata tttgtaaatg acatatatgt caaaagaatg 301620gttttcagaa tattttacaa aaaacttaca actcaacaag aagacaatgc aattttttaa 301680atgggcaaaa aaaattgaac agatacttca caaaggaagt gtacaaatga ccaataaaga 301740cataaataaa tgctcaaaat tattagtcat tagagaaaac ctgtttaaac cacaatgaaa 301800taccactgca agtcactaaa atggctaaaa tttaaaaggc agacaatatt aagcgctgtt 301860gaaaatatga agcaattaga actgatgttg ctggtgggaa agaaaaatga tacagccatt 301920ttggaaaaca atttctcagt ttattattta tttaaacaca tcgtaaaagt cagagatcct 301980acttttaggt atttattcat tgaaataaaa tgaaaacatt tgtctacaca aagatttata 302040tataaacatt cacagcagct ttgctcataa tagcctatgg tagcagcctc taaggtagcc 302100ctgaatgacc cctacctcct ggtattcata cccttatatc agcttccttg aatgcaggcc 302160agacttacta atttaattct aataaaatgt ggcagaaatg atgagatgtc acttccaagg 302220ttagattaca aagactgtgg cccagcctgg gcaacaaagt gaaacctttt tgcaaaaatt 302280tagaaattag ccaggcatgg ttgtgtgcgc ctgtagtccc aactacctgg gaggctaagg 302340tgggaggaac atttgaactc agaaggtgga ggctgcagtg agccgacatt gtgccactgc 302400actccagcct gggtgacaga gcaagaccct gtctctaaaa gaaaaaaaaa aaatgtggct 302460tctgtcttga cagctctctc tcactctctt ggagattgtt tatgctgagg gaagccagct 302520gccatggtgt gaggcagact cctggaggag cccacatgtc tgtaagtaga agcagatctt 302580ttgaggcctg tcaacagcca cgggaatgag cttggaagca gatcccacct cctccctcac 302640acaagtcgag ccttcagatg agcctgcagc ctttgtcgac accttgactg cattctcatg 302700agagaccttg agccagagat acttagctaa gccatgccca tggactcctg acccacagaa 302760actgtgataa taagtttgtt gtttcaagct gctaacttat ggagtaatat gttacacaaa 302820aatagctaat atatagctca aaactggaag caacccaaat atctattaac tggtagataa 302880acaaactact catttccaaa cttatttcca aaactggaac actacttggc aatcaaataa 302940ttaactatgc attaagtgta acaacctgga tgaatctcaa aggcattatg ttaagtgaaa 303000caagtgagcc acgtaagact acatactgtt tgattccctc tatatgatat tctagaaaag 303060gcaaaactat agtaatagga aacagtgagt gatcacctag ggttgaagac aggtgaaagg 303120ggattgactg caaagaggca ggaggaaacg tcttgggaga tggagatgtt ccttatattg 303180atggcggtgg tggttacaca actgcacttt tatcaaaact tacctaactg ctacttaaaa 303240taggtgtatt aatattttta ctgtatgtaa attatacctc aataaatttg atttaaaaaa 303300caggccgggt gtggtggctc acgcctgtac tcccagcact ttgggaggtc gaggtgggca 303360gatcagctga ggtcaggagt tcaagaccag cctggccaac atggtgaaat cctgtctcta 303420ctaaaaatac aaaataaggt cagcgtggtg gcacacgcct gtaatctcag ctactgggga 303480agctgaggca gaagaatcac ttgaacctgg gaggtggagg ttgcggtgag ccaagatcgc 303540accattgcac tccaggctgg gcaaaaagag tgaaactccg tctcaaaaaa aaaaaaaaat 303600tagttttcta tttttataat gtcattttat gaatgtatgt ttcagttatt cttacaacag 303660tagtatttgt ggaattatct ttaggttaca aagacctgtt ttaacaaatg caatccaggt 303720agaagggtat agtgcaatta aaacaaacat ttaaagctta gttgagagtt ctgacacttc 303780tttaaaagtc aatataaaaa ctaatacctg aatatgctag aaaatggaaa agggcatcct 303840aaaagtaaga ttattgcaca aatgaggatt tcacatagga ctagttattt gggacttact 303900tcccaggagg agattaggac acatcgggac acatagaaat aaacccgagc cttccttgtc 303960cctacttcct ttcctcagtt ctagctcaga aagaaagtct agcaatttag aatgtcctga 304020agtttgagag atgcttttac atttttacat gtgtatcagt agaaggtagc aaaatcccag 304080ctgcttttgc ctgagctcac ttttgtacag tttttttttt taactcataa taagcatttg 304140aaggaaaaaa aaaaagcatc attcctctct cttgtcttgg taaagtcctc aaaaatagtg 304200aatcagggag gtgataaaga gtttaaaaat gacaaacttt ggggatgtgg aaagttaatc 304260caagtggggg aaggcaaaaa aaatcacaag caaggggaag aaaagaaaaa aaaatgggta 304320gaaatgcagc atctttacaa ctgttaccgt aagaaaaaaa tatgccaacg attctcaaac 304380gtcagggagg tctgaggtca gcagctcact taggaacaca ctgtgccatt ccaaagataa 304440aaaaaggagc tgaatcacct tggaagtctg attctgtaaa cactgttacc aaataagctt 304500ttctctaagg gattccttct catggcagaa taagagaagg ggaacacacc tgcgcaatgc 304560aacttcccta gtactcagca tccggaagat gttttgcagc cgaggcctca agtgggaaaa 304620cactttcatt tgttttgact ttgtaagcca gcatggacac gtggggcatg tgtggagtac 304680cagcaaggac aggaaatttg gaatcatggt gtgttagaag tggaaggaac catgggttcg 304740ttttctattg ctgtgtaact aattaccaca aacttaatgg cttaaaacaa aacaaagtta 304800tctcacagtg tccaagggta aggagtccag gcatagctga ggtgagtcct ccttacaggg 304860agccacaagg atgcagtccg gtgccatctg gagcttgggg ttctctttga agatcattca 304920ggttgttggc aaaattcagc tgtaggactg ggttccctgt ttccttgctg gctctcattg 304980actctcagct tctagaaaaa gcctttgggc cctagctcca tggccctctg acaatatagc 305040cgctttctca agaagaatct ctctgcactc tgctgcaggc tgagtagccc ttatctgaaa 305100tgcttgagac cagaagcatt tcagattttg gactttttca aattttggaa tgtaagtatt 305160atacttaccg gtggagcatt ccaaatccca aaatcccata ttagagagcc ttataatcac 305220gttaatcaag tacttatggg agtgactacc ccattacctt aatcatataa cgtaacctag 305280tcaatgaagg gactatccca tcatattcat attcctgccc acattcaagt attattcttt 305340caggtcatat acaccagagg atgggaatat tggggggcat cttagaattc taacaaccaa 305400aaccatcagc actatcctat gcacattctt tttttttttt gagacggagt cttgctttgt 305460cgcccaggct ggagtgcagt ggtgcgatct cggctcactg caagctccgc ctcctgggtt 305520cacgccattc tcctgcctca gcctcccgag tagctgggac tacaggcgcc cgccaccgtg 305580cccagctaat tttttgtatt tttagtagag acagggtttc accgtgttag ccaggatggt 305640ctcgatctcc taaccttgtg atccgcccac attcttattt catggggaga aactgaggtc 305700catagaagat atactaaatt atccaaaaag tcataaagta gttatcagga cagtaagagc 305760tgaatcctgg tctccaaaat ccatgtctac tgcttgccac tctctcaagc ttccttctgc 305820cactgacatg gttagcggta aggttgtatt tcaggaaagg aggaaaaaag caccaagaca 305880atcagctaag ccacgaatct ttatgtagat gctatgagtg gcagaaaacc tggaagaaca 305940cttacctgca cagtgctagc atggtgactc agtgtcctca aagagtcata gtcagcagga 306000ttggccagca aatgactggt attttctatc caagcctcag tgctcttcag aaggtcacta 306060tattcctcca tcttaactgt ggcctgggaa agagatgcag gaccaaactt agtcagatgc 306120cttttactgg agctgaatga ctcctagatg aagagtcaat agctgttagt caggggtgtg 306180tcaggccttg cagatgtctg ctccccatgg ctggggctac ttattcacct gctctaggct 306240atgacacttc tgacttttaa gactaactct gaccataggt ggaaaggatt taatcagttc 306300tactccaacg ttatgggcct cagcaaagtt ctgggttagc cttgcaagta ttctggttct 306360cattttatgc ttcattttaa aaactggact gtactttgtc ctcctgttca ggaccttgcc 306420ctacagttct tctaagacta ggatgctgcc tcactcttgc taaatcacag acacaactca 306480gccaaccaca gctcctattt ggaaggaggg tagagagggt atcttttccc cgaatgacag 306540tctttctcat tgttgtctcc cttactagac tcctgagacc acctgcccta aattcccatt 306600actggtcttt atccacctgt tggggagcct ggacttccca acagtttgcc tggctcttgc 306660tgtggtcaac ttctggattc ttggttctgt ctgcccaatc agacttcctg cctgcactag 306720cattgaaatc tgtctcctca gcacccacac aagcctacca tggccccagc ttcaagtcca 306780aaactgataa ttcaacagaa actgactgtt tctgaatata ttgttttgct ttaacaccat 306840ctcaatacca ccacatgata tgaagctatt acgagtcaca gccatacctt attcaactgt 306900gaggttctac actctgctct ctgtgatact tgctgatact gctggaaagg aatcatcaag 306960ttatccatcc attcttgagc ctgtcctggg tcctgttcaa caatgtcctg aacttcagaa 307020tccagctcat ttagtttgga atgccataga tctaactcgt cccacatttt ctggagaagg 307080aaatcgtaag tcaacttgaa tgaagaggtc atagtttatg gaggcaatgc ctggttttca 307140cacagtattt acatttataa atgctactaa aacatcacag aaacaacatc acatgaacag 307200ttgttaattt atacccagca atttgtgatc taaactgaaa aacacttttc ctgagctctc 307260ctaactacaa ctgtattcat ttcactacaa attagctgta ggggaacaga aagacctgga 307320catttttaga gatgactgac acaaacaagg tcaatatagc cctcatctct caagagtgac 307380aagcaaaaca ttctgtttaa aaagggcttg gggaaagact tgaacctatt tacactagtt 307440catacacaca cacgcacgcg cgcacacaca cacacacaca cgctctatta atctacaaaa 307500taatttcaag tgaaaaaata aaacaaagaa aaatatctgg gctgggtgca gtggctcaca 307560caattttgga ggccaaggtg tgtgggctgc ttgaggccag gagttcaaga ccagcctgga 307620caacacaggg aaacaccgtc tctacaaaac ttagccaggt gtagtggcgc acacctgcag 307680tctcatctac ttgggagact gaggtgggag gatcaattga gccagggagg tcaaggctga 307740agtgagtttt gatcacacca ctgcactcca gcttgggtga cagggtgaga ccctatctca 307800aaaatttaaa aaaagaaaag aaaattatct gctgacttgg gatgaatgca gaggcagaaa 307860gctctagaaa catgtattag gagcaactgc ttttgactca agaaaccaat aggttggatt 307920caaatctgaa cttcgctggg cagaggaaaa tttcaaaggt aaaatattca attcttaatg 307980tttgcttaag aacatttttt tccctatctt gaggactagg aaaatctaac ttgttaaatt 308040aggttcctct gcttaagatt tgtccaatag tgagccatac tctgacctat tttaaaagcc 308100ccaaattatt aaaacatttc cttaagacat agattagaac aagaatcctt tccctatgct 308160ggagtttact tgcgaattaa gaaggagaat agtgtttatg tacagttttg cctaagattt 308220tattttgtaa gatattttac aagggaaata aatttggaat ttttattttt atttaacctt 308280tgcagtctaa aaaagagctt taatattttt catattctat tgaatgattt ttagtatcag 308340tatccccaag ataagatcct tactttcctt tacttgtgaa aatgctagtt cacaatcaca 308400ggctctcaga agtttgggta tatgcccacc atggaaaaac agaattattt ttttgagaca 308460gagtctcact ctgtcaccca cactggagtg cagtgacatg atctcggctc attgcaacct 308520ccatctcctg ggttcaagtg attttcgtgc ctcagcctcc cgagtagcta gaattgcagt 308580cttgtgccac cacacccagc taatatttgt atttttagta gagatggggt tttgccatgt 308640tggccaggct ggtctcaaac tcctaggctc aagtgatcat cccaccttgg cctcccaaag 308700tgctgggatt acaggcgtgg gtcaccgcac ctggccaaga gaataatttt taaatgcaac 308760tttccctatc aagcctctct aggtgatgcc cctgtggatt tggtttacaa tttctgtgct 308820tcagattaat tcaaagaact caccctgtgg tgactgatga acaaggcagg agtgggtcag 308880aagtaccaaa tgctgaaggg agttaaagga ggattttacc ttttgaattt cttgagcttt 308940ctcaatattt ttgcttttct gctgcaacat cttttcaaca ttatgaagcc cattgttaat 309000ttcttctatt tttctcctca ttgcatatga tggtgagctt tttaagactt cattgctaat 309060ctgtgaaaga gacaaagacg catttggctt agagaggttg tctgcacttt attcataggt 309120tcgaacaaca aaagtggtag tcactctgtt ttaacttgta aaaatttttt aagggctggg 309180ggaagaaatt ctggcaaatg cattaatgag aaggtggata ggaagactaa taaatggcag 309240gtgtgtttac ttcacctttt aaatctcatc ttaaataaaa taaaaattgg ttttttattt 309300gtactgtaat acatgtattg taacatgtat tacagatata tgctgtgttg ggcaaaatta 309360ctgagtggat tgtgtgaaac atttccatat ggatcaaatg atataaatga attcctgggg 309420gataaattat ttttatacaa tctttccatt ttatgagaat ctatctgcat agatctgttt 309480tcagtaaaac caaacttact ctcaagctga cttttttaaa aaaatgtaat tccaatcata 309540gtattcattt gggtgaaaca accttctaaa attgccattt cttgaggatg cacagagctt 309600aattattctc tgtgtaacca tttgtagatg ttttcccagc catcagaaac ttagctactg 309660aacagcgtaa ttttttttta tctaaacgat tctcttaaaa agaaacaaaa aaaaccttga 309720gctaggaaag ataataaatg aatgttgaat gtatcacttt ccaaatacct gactgatgtg 309780tgttcttgtt ttgctatttg ataaggcttt ctccaagcag ctactaaata accaaataat 309840actttatgct tttgtataat cctttgtttt gtaaattaaa aaaaaataac tctatctgta 309900taaccattga gactaaaatc atcctgtgca gacagtatac agatgctgaa actgtaacac 309960agactaaatg acttgctaca gtcatatgtc aaggaagtag ctgaacttgt cccaagaagc 310020gcccaagtcc ctagattgag tcccaggctc tattcacaat atcaggcctt gatactttta 310080tatcatgtct ttattcccca gaccatactg cagtcaatat tattagaaaa aaaaaaatcc 310140cactttggtt ccatattagc ttttcatggc tggctgtgtc acaaattacc acaagaaaag 310200ttttctgata ttttgagcac ttattctcct ggcagaaatg tataccattg agatacatta 310260ggacagatca tgatttagga aacatatgta gccatttgaa ttatacacaa tgttactatg 310320tctgtccccc gggatccttg aacttccttt agctgtatca cacaataaaa attagttgct 310380gtttgttaca aatatagagg gtcatagcgg aaattttcta ccttgaattt gaacgactga 310440ttttgattat taatttactc tgctagggat tttcataagt attcatgagc ataacattta 310500gtttctctca aaatatttag tattttcctg aaataaaatc aatagtaacg agatccttga 310560gaaagtacga aataggtttt cttgtgtgtt aactaaaaaa gatttgttat aaatatacgt 310620taagagttta catatttttt acatgtgaga ttttcctgag aaatctgtca tagaattaat 310680gacatattct agcaactaag tttgaaatct gaattttttg tgtttaagat ctattttatt 310740tatcaagttg taagtattct agacaaaaaa aaaaaaaaaa atccttctgt ggataccaga 310800ccaaacagaa ttttcttctg tttgatatgg gctgtctttc tacaatctaa tcaaaatatc 310860ttgctaagct cctatgtttc tagtattata aaatattttc caccttttct ttaaatctgg 310920actttcaggt ataaagattt gctcaggtac ttttcatgag aattctgtta acatcatcgt 310980tagacagaca ctacatacta agtgctattc ctagtgagtt gtgaaatttg tattcttatt 311040gccttagttg taatgtcaaa atgtttcaaa gaatgtattt taaagcaaag tattaacatc 311100caaaatgttc aagtggttgt acagtggttg tacaaaagca aacaaaacca gtaaaaaata 311160ttatataact gtattataaa atgcaagaga agaataagca ttttgacaat taaatacata 311220taaatcatct aattctgtta tcttatgtca catatttaga gcatctttat cttagcattc 311280aatagacacg atggtcactt aactcttaaa agaagttata gctgaaatcc tggcaaatgg 311340aatttgacat taaatgatat tttcctcttg atagtcattc agctccgtaa ataataacag 311400tagtacagac attattatta ccttctcatc tatgtcttct aaagcttttc tgcattcttc 311460cacctgggat tcaatctctg cagggactat ggtgtacatt tcggaatact tgattcgcag 311520tttttccata atattctcaa aagttagttt ccctttctta aggatgcttt gaagctccta 311580aaagcattaa gagagttgca atactcacga gactggggga aaagtttaat tctaaaaagt 311640gaaagcagcc ttccagcaca tccttatgat ttaaaggagt gcagtttgca tgacaaaacc 311700ccactagtga tacaagctga ttaagcccca gagacactca gtttagtcag atgatgcttt 311760ctaatttgta agaattaaac ttgatatctc ttcatagtgt cacaccatta tctagaaaag 311820acattacaac accccctgct tctaaagtac ctttaggatt taattgtatc tccattgcag 311880tcctgatcta aaaagctgca agcagcaaaa aaaaaaaaaa aaaagtttca aagaagtttt 311940ttgttgttgt tttaacgtgt tgctatttct acttcttcct ccattatcat cacaatgatt 312000taaggccact aaaagcccca cacttggagc agaagtgtct actttcatgt aattagacag 312060ccctgcagag ctcaactgct ttcaaaaggg aaggttccag ttccatacaa agaatgtagg 312120tttttaggag ataaaagaaa cctgaacaaa tagtctattg gttgtggcct agaaaccaaa 312180tctgatctac cttctcctgc agtaattata aaggagaaac tcatttgtat gggaaagttt 312240cccaaagatc aagtggaaaa atacttcttg acttgactgt ggcttttttg ttgtttgttt 312300tgtttttaca gtagctgttc agtagcatta tttgtgttca tggattgagt cactgtactg 312360aagaatgatt acaattttaa atgactctgt aatgtgtcat tgttttcctt ttcttaatcc 312420tcagcagagg acaaatctag agagagaaag ttagagaaca atgccattca aagatggggt 312480ttaataagta agtgtcatcc cgttttatct acacctagtc caggagtaca ggctttcatt 312540cagaggtaca cttagaaggc ttccccatga agactgcttc tttactttcc aatataataa 312600ccaaaagttc tgtttagaca gcatgtgact tttttttttt ttgagatgga gccaggctgg 312660attgcagcgg cacgatctcg gcttactaca accaccacct actgggtcca agtgattctc 312720ctgcctcagc atcccagtag ctgggattat aggcacacgc taccatgcct ggctaatttt 312780tgtattttta gtagagacgg agtttcccca tgttggccag gctggtcttg acctcctgac 312840cccaagtgat ctgcctgcct ggacctctca aagtgctggg attataggtg tgagccacca 312900tgtccggcca acacgtgact tttgaataaa cttccaaaca tacttttggg ctgtcactat 312960tggactggaa tgaaagagga gattcttctg aagaaatgag attccagatg acctgtaggg 313020tgtacataga ccctgtcaga gacaaggcgc catgtcctac taaaggagta atgtaacata 313080ctacttaaga gtggtctgag gcccttgttc accaaacaca ctggaaacat agttgaaaga 313140aggctgcttg gtccagactg gcctaccttc cagccttgtt tcaactctgt tctctgctct 313200ccagccacac tgatcttctc ccttggttct gcccatacca tgccacaggg cctttgccta 313260tgcctctctc tctctcccca gaccgctccc ccaaacccag ccactgagtt atctctaccc 313320caaggacttt ggatacaaga ctgtgggcct gtacctgcca cattcactaa ttcattcctc 313380acttacctca aattgttctg acttttctgg gtgatcctgg aatgccatat tttggaatga 313440agttgtggtc tgttgaaaga aattcttcaa agcaattatc tcttttgtga aggaatgtct 313500ttcttggatt tcagtctgca ccaattcttt atttttctga actttctgaa gaagcctaaa 313560agagaacgtc aaaatataat tgtttcttta tcctgctctt ctatgaataa aatgaaacag 313620ttttattgca acaattaatg aaatgcctga atagctacta aaggatggtt atttcctcta 313680agaaaaaaag taacaatttt caataatgga tgtaccacat ataaaaacag gtacaaaatc 313740taccacttaa tcaaaaacct ctccaaatct ttcgcctcaa atactctctt ttcacacttt 313800acattgtaac tgacatttct gtcctctctt tttctccacg taatccttga gctcacatga 313860aaatgactta gaggtgtgaa tggaatcaca ggtgggctct tggggacaga caatcccact 313920gggcttcttc tcttaagctg acccattttg ctatggatgc agcaaatatc agaagaggca 313980ctgaagtggg taaaatcttt cctgagggtt atttggtaag tatctatgat attgtgatat 314040aataaatata tacttgatct ctgctgtagt tcctgagaca gagctcttaa tacccttgta 314100gataggagtg ctagctagga gagtcttttg ttctaatact tgatttttga ccagttcctg 314160acacagagct cctaagccct ttgtaatttc ctgagtgata ggagcatctt tagttctaag 314220aaggcaactc taggtgggat cctgagtagc ctcaggatga gggctggttg ccaggggaac 314280caactatgtg attaaaaggt tggaactttc agtaccaccc ctacctccaa cacacacccc 314340caacctctgg gggaggggac agaagctgaa ggttgagttg atcgccaatg gccaattaca 314400taatcaatca tgactacata atgaagtctc cataaaaaaa cccaaaagac agggctcaga 314460gagcttctgg attgctgaaa gcctgggggt tccaccacct agagagagca gggaagcccc 314520aggccccttc ctataccatg ccttaggcac ctcttccatc tggctgttta tctgtatcct 314580tcattatatc ctttattaat aaactggtaa acatgagtaa agtgttttct tgagttctgt 314640gagccactct agcaaattaa ttgaacccaa gaaaggtatc aaaggatccc ttgatttata 314700gcctatcagc cagaagtgta ccagcggggc gcagtggctc acgcctgtaa tcccagcact 314760gtgggaggcc gaggcgggca gatgacctga agtcaggcgt tcgagaccac cctgggcaac 314820atggtgaaac ccctcctcta ctaaaaatac aaagaattag ccgggtgtgg tggcacatgc 314880gtgtaatccc agctactcag gaggctgagg caggagaatc gcttgaactc aggaagccga 314940gtttgcagtg agccgagatc atgctattgt actccagcct aggcgacaga gcaagactct 315000gtctcaaaaa aaaaaaaagg agaagtatag gtagcaacct actactgatg attggcatct 315060gaagtagagg tcctcttgtg ggatggattg agcccccagc ctgtgtgatc tgatgctgtc 315120tccgggtgga tagtgtgaga aatgaattgg tgtctgctgg agaactgcct gatgtgtggg 315180gaacccccat aaccaacatg gtgtcagaag tgctttgttg catggtgtgt aagggtagag 315240agaaaaaaca agtttgcttt ttcttcagag cacctctagc cataaaacta ctatattctt 315300tgacccagtg attctacttc tcactatctt tctcaatgaa ttagtcacag atgaaaatat 315360agattctggc ataagaatat tcactgcagt gttgttatga aaattaaaaa aaagtaattt 315420aaagattcct tattaggggc ttggtttaac acattatggc atatcttcat ggtagaatat 315480tatacagcta gttacatttt ttatttttat tttttgagat ggacgtttca ggctggagtg 315540caatggcatg atctcaactc actgcaacct ctacctcttg ggttcaagtg attctcttcc 315600ctcagcctcc tgagtagctg ggattatagg cgtgcgccac catgcccggc taattttttg 315660tatttttagt agagacgggg ttttaccatg ttgaccaggc cagtctcgaa ctcctgacct 315720caggtgatcc atccaccttg gcctcccaaa gtgctgggat tacaggcgtg agccaccatg 315780cccggccaca gctagttaca ttttaaaaga acatttaatg gcataggaag acaaacatga 315840tttgctggta aattactcag cagaatacaa aacagtacaa gtattttgat ctctatttat 315900ttaaaaacaa aatcatagta aaaaaggaga aaaaatgatt ggcagtggtt attgctgagt 315960ggtgacatta aggtagattt ttatattaat ttttgtaatt ttcacatttt ctgaatttcc 316020ttttttttga gacagagtct cactctgtca cccaggctgg agtgcagtgg cacgatctcg 316080gctcactgca agctctgcct tctgggttca cgccattctc ctgcctcagc ctcccgagta 316140gctgggacta caagctcctg ccaccacacc cggctgattt tttgtatttt tagtagagac 316200ggggtttcac cacgttagcc aggatggtct tgatctcctg acctcgtgat ctgcccgcct 316260cagcctccca aagtgctggg attacaggag tgagccaccg cgctcggcct gaatttccta 316320taatgaatat gcattactaa actagaaaaa gggcaaaaac aaacaaacaa acaaaaaacc 316380cacaaatgat aaaaaaggaa attggaagga cattcaccct cattaatacc aggatggaaa 316440atgtgtgctt aattatagtt actaatctgt tccatccctt tttataccta tcagtgttgt 316500ctcacctcaa ttctattgta tcatttgcca ttattctata cttttagatt ttctaaattt 316560tgagccataa ttgtcaagca catgtcctta atagtttttc ataaatctta cttgttgcat 316620cgttctttca tagaagtaat ttcttgaaat gctggaacag tttccacagc ccctgagctt 316680tcaggaacat tctggatact tctgatgcgc tgaagaagta gagtcagtaa cagctgttgc 316740ttctccacgt tctctccata ttggcataaa cagtctagca gctcagagag ttcctctgtg 316800gtggctgcct cttttgtttt ggaaatttca gggagttctt cctgaagatt atctaaaata 316860attgcctctc gttcaatcta attcaaaaaa caaaaagaac taaaataagt aaaatataca 316920attacatttc aggacctgaa gtagccataa tttttgtatg ctctctcttc aaagggttcc 316980tagtgccata tcctagtctg ttttcctata gctgggaata ttaatcctct gctatcagaa 317040tcccataatg cccagctatt gctcatgtag atgtgtgact gaaatggagt actgctgtgg 317100tgggctgaag gaaatgctgt tgaatatctt cataaataat gtatttctcc ttcagatttc 317160agatggataa agcaacattt ctcctgagat aagcacatga gctccaccaa actgctttca 317220ctaagtgtaa gaggttgggc aactattgga tatggaaaat ttttctgctg gtctcttttc 317280tttcacttcc aatccctgta tgctaaaaaa gtaaggccag caggttctca ttaccctgac 317340acacaacaaa ggtaaagcag ccagaattac caaaccagtg aagtatttat caatagtggt 317400gtacacagaa ctcagaaata cataacctat tacatcacat atctagaaac tgggagttcc 317460accagccctg aactcaacta agatgtagaa aaaagtctca aaggcccctg aatcaggtca 317520ttcttacgcc tcccctcgaa tcccctggcc cacccctcgg ctgcagtact catttatcaa 317580atcctgcctc agtgatggtt aactctcctg ctccacgttc aaactcattc atggatcttg 317640cagtcatgac attacaggat atcactccag tcatctatgt ttatgatatc atgctaactg 317700aaatggtgag gacctgaatg tccagtcaga catgccagag gctgagaaac aaagcctgtg 317760aaaattcagg agccccagta tcaggggtct agtggcctgg ggcatgccag gacttctcct 317820ctaagataaa ggtcaagttg ttcaatctta caactctacc actaaaaaag agacacagca 317880ctgaacggac cttttggggt ctaggaggca gtatatgcta tccttgggat tactgctcag 317940atgcatttat cagaatgctg ccaatgtcga gaggggcctg gggaaaaagc accgcagcag 318000ggttgggctg tggtataagc tgccctgctg tttgggctgt atgacccagt tgggtaccca 318060ataactagaa tacacggatg cagatgtaga ctggccactg tccaagagac ctaggtgtgc 318120gcaatgggtg cttctcatca cctcaaacct aaattcagtt gaattagaag tcctagttct 318180ttgccagaca gctacactta caccaggaaa tgagataagg gttccattgt atcattttct 318240gtccccctgc tggaaactag catgcaaata aagaaattgt tacatagatg ggggaattgt 318300acatctaggg tttccaatgt gcatcagggt tgaaaaaact gtgtctgtaa tccaggattt 318360aatggggcac ctcttggtgc ttacacacca gaaactggca gatttgactt gataataacc 318420agaaatgggc agctgtagca agcacaatcc agtaagagta aaaagaataa aggctcagac 318480ctttcctgtt tcggctcccc tcatctccca ctcctgttcc ctgggatcac tttccaaaat 318540aaactaccat ttgcaagcct cagtcccaag ctctggtgtc cactgtgaag ttggtaagtg 318600attgagaagg gggaatacaa gagggagaac aatctctttt tttttttttt tttttttgag 318660acggagtctc tctctgtcac ccaggctgga gtgcagtggc atgatctcgg ctcactgtaa 318720cctcccacgc cctaggttca agcaatcctc tgcctcagcc tccggagcag cggggattac 318780aggcacctgc caccatgcct ggctattttt tttgtatttt tagtagagac agggtttcac 318840catcttggcc aggctgatct tgaactcctg acctcgtgat ccacctgtct aggccaccca 318900aagtgctggg attacaggcg tgagccactg ctcctggcca caatttttat taggacaata 318960attttacccc aaagccaggc aattcttcaa cttgggaatg aataaacaaa ctgaattcac 319020ccacccaaag caatactact caggaataaa gagaaatgaa ctactaatac aagcagcagc 319080cagaatttca agtgcattat gcttagtgaa aaaaagtaaa ctcaaaaggc cacaaactgt 319140aaggagtcca tggatatgac attctggaaa tggaaatact atgagaacag aaacagatta 319200atggttccag gggctggagg ttatggaagg cactgaccac aaagaggaac gagggaaact 319260tctagggtga cagaattgtc ttaaaccttg attttgctgg tgatcatgtg accgtgtgca 319320cttgtcagaa caacatattt tacactaaaa agtgtgaaga cagccctagc atcagtaaaa 319380tagttgtcaa aaagcctgtg cattcacttt tccacctaga ataggctttg caataccttg 319440atgattttcc ttagacatgg gatcctaatc aaattggtta taaaactttc aggttcacgg 319500tcaattgtaa agtccggtct agagagggag aaggacccat agttgcatca taagcaaatc 319560caatgaagga gaccaaaaaa cccagaacca gcagaaaaga gacccaacaa agaaatgact 319620gttggtgcaa gtggaagaag ccggcctcac atctccaatt atctttgaca atgtgacgtt 319680cttctagcac attatgatca ttgaagcata aaatgatgat tctgaataac acaagtgaat 319740agttatagta aagagattct gtttaagaaa acagggaggt aaaattttag ggggacacaa 319800gttctatcaa aatttaaatt tagtttttaa tttgtaaact tacaaacagg catttaattt 319860ttaaaaaagt ctgctaatat aaactttatt tcataaacac tggattatta tgttactata 319920gatgacaggt ttaaagcaga aattcacttt gtcatatata aaagcaccaa tcatgctaac 319980agaagcttac atgtatgggc tgttagatga tatacttttt attgaatgga taaaagctac 320040actaaaatct tatatcaatt tgatgtatgt aaatatcatg taagttgaca gattaatgac 320100taagcaccag cgtctaagtt tgaatcctct tctacccctt gtgaatttga gcaagttact 320160cagtctcact gtgtctcact ttcctcatct acaaagtaag gataataaaa atatcctcac 320220agaattcttg aatagtgctc tgaactatcc tgtcacacaa taattgctca atttagtagt 320280tgtttttatt attgttatta cagggaagca tttttttttt gcttgtgcta ctctttgaaa 320340aatattttgt tagcagattt tgtcaaatgc ttatgtgaat aattagaaag ttctatatga 320400atggagtctg aattaataac acataattgt aaatgggtat atagcaataa tgtgataaaa 320460taaaaggagc aaaatcttag aattagccca gacctgaatt tgaataagag atgtccctca 320520acaaatattt actaagtgcc cactaagcgc agggccctag ccaggttcca aggttacagc 320580agtgagcagg acagccccag ccccagccta caggggttta cagcttaatc aatctctacc 320640tggggacctt gtgccactac tcaatctatc agtgcttcag tttccccatc gataaaatgg 320700tgataaaatt tacttcccag ggacaatgca aagattcagt gagaacaggt gtgaactgcc 320760ttctcagcac agcatagtaa ctcaataaat ggtaccacta tggacaggca agcactggtg 320820ccagcagccc caaccaaaag tgtaatttac aaagtttatt ttaacatttc aagagactat 320880tcactagtca ggcctcacca ggaccactga cataaaattc atttttaagg ataatagtta 320940taagcaagat aatacaatag tgctaagcat ttcatcctaa gataatttta cgttgtgttt 321000ttgtttgttt gtttttgaga cagtgtctca ctctgttgcc caggctggaa tgctagaata 321060cagtggcatg atcatggctc actgcagtct tgacctctca tcaagagatc ctcccacctc 321120agcctcccaa gaagctggga ctacaggcat gtgccaccat gcccagattt ttttttattt 321180tttgtagaga tggggttcac tccattgcct gggctgttct agaactcctg ggctcaagcg 321240attctcctgt ctcagcctcc caaagtgctg ggattacata ggtgaaccac tgcacccggc 321300tatctttaac tttctaaatt aattttcttt ctaaatatag ttcataccta aatcctgaac 321360caaggttaaa ttaacatttg tgattgatat atatcatgca ggttttgaga ctattttcaa 321420aaagaacatc ataacctcat atatttttag gtccacattt aacaattaca gttaatttcc 321480atagttgaaa attatagata atttcaaaat tatttggtga ttacacatat tacctaattc 321540taaaccttta gtataatgta gagacagtta tttctgagag gggttatttt cacaataaag 321600attgtaaaac tcaaaccagc tagtagtcac ttattaataa gaattatctg ctatcaataa 321660aggcactact ataaaataat ctgaataagt tcttgcaggt tatcttaata aggcctgaca 321720tctataaaca tttatgtatc atgtataagt gatgtgcaca aatggtatag gctaaggact 321780cgttttatag aaaaacaagg tgaaagtaat aaaacaattt aaataacatg ttagaaacta 321840catggtttgt tgaaaatgaa gcactcactt cttcactgac aaatttccat tcctgcttca 321900gttcttcgca ccacatctcc cactctttag cagcttccag caaactcagc catttctccc 321960acagagccga cacaatcttg agcaaacata tgccatcatt ttctgtgcac ctgagtctca 322020agagtctaag gatctgtcgt agtttcatta actcttcttt ggttgatata agatttgaca 322080ccaaggcctt aagaaagaaa agaaaaaaaa taagagggac atactattac ggttttcctg 322140aaacttacta actcttttaa atcctgaaca attaattatt tcaatcttcc ataagtattg 322200catgatccac ctcttatcct tttagaagtc aatttagacc agatgagaat tatctatgta 322260ttattgtatt ctgcatgaat ttgggattac agggacaaat aatgacccat aaaccatttt 322320tttccaagaa agaacatact ttcaaaagca aatatttgcc agacgcagtg gctcatgcct 322380gtgatcccag cactttggga ggccaaggtg ggtggatcat gagatcagga gatcgagacc 322440atcctggcca acatgttgaa accctatctc tattaaaaac acaaaattta gctgggcatg 322500gtggcatgcg cctgtagtcc cagctactca ggaggctgaa gcaggagaat tgactgaacc 322560tgggaggcac aggttgcagg gttgcagtga gctgaaattg tgccactcac tgcactccag 322620cctgggcaac agagtgagac tccgtataaa aaaaaaaaaa aaaagtctat aagtggctgg 322680gcacagtggc tcatgcagca ctttgggaga ctgaggcagg aggattgctt gaggctagga 322740attcaagaca tagcaaggca acatagcaag accctgtctc tacaaaaaat taaaaaatta 322800accaggcatg gtggtgtatg cctgtagccc cagctactca gcgggctggg atgggaggat 322860cccttgaggc caggagctca aggctgtgat aagccatgat tgcaccattg tactccagcc 322920tgggtgacag aacaagaccc tgtcttattt aaaaaaaaaa aaaaaaaaaa aaaaatctaa 322980aagtacaaca gccctcatta tctgggtttc tcctttcaag gtttcagtta cttgtgatta 323040acaacggtcc aaaaatacta aaaaattcca gaaggaaaaa atgtgtaaga tttatttgta 323100taaaatgtat gttttatttg tatataaata tataagtgct attctgacta gtgtgatgaa 323160atctcaagcc atctctctcc atcccacctg ggatacgaat cttccctttg tccagcacat 323220catgctgtat atgttcccca cctacgcgtc atttggtagc cttcttggtg atcagatcaa 323280ctgttgtggt atcaaatgct tgtgctccag atgaatagta gcccaacgct acctcaccat 323340ggctatatca tttcatttca tcacttcatt tcattacctc acttcatttc atcacctcac 323400ttcatttcat cacccaggca ttgtatagtc tcatgtcatt acaagaaggg tgaaaacagt 323460acaatgacat attttgagag aaagagacca cattcaccca acttttatta cagcatattg 323520ttataatttc tctgttattg ttgttgacct ctgactgcac tttaatttat aaattaaact 323580ttatcatagg cgtgtacagg aaaaaaatta tatacataga gttcagtgtt attcacagtc 323640tcaagcatcc actgggtgtc ttggaacata tcccctgtgg ataaaggggg actactgtac 323700attttgttat ttttaagtct cacattttac taagtagttc ctttgcacaa tacttcctat 323760atcaaccacc aacacaatat tgaaaggttt ttcttggttc gcttttgttt ttggggaatg 323820ttaactacag atgatttaga aagtcctcat ccaaatccta ataacataat accacagatt 323880ctaacagata aaaataagta aaagctcaaa ttgctttgta acagtctaag aagtaaccag 323940aattcagagc ttatgacagt tgcagtaagc gatcagtgga caacaaggct ctgtcactga 324000tccagcagta tccctcaaag tcacatgaga ggtgatgctt tagaaaagcc atcagtgagg 324060acaggatgct attgggggat gactgtctgt gaacctgatt aattaaatct ctcagtacca 324120ctgcttcatt tccttccttc tggcttttta agcttcaata tattacataa ggccaggcgc 324180agtggctcgc gcctataatc ccagcacttt gaaaggccga ggctggagga tggcttgaag 324240tcaggagttc aagagcagcc tggtcaacat tgtgaaaccc cgtctctact aaaaatacaa 324300aaatcagctg ggcatggtgg cgggcactgt aatcccagct actcaggagg ccgaggcagg 324360agaatcaaat cacttgaagc cgggaggtag aagctgcagt gagccaagac tgcatgactg 324420cactccagcc tgggcaagac tgtgtctcaa atatatataa aacattactc tctggctttc 324480gatttgagga atattcatgg atatttaact cactgtgaag atttctacgc gtcttaacag 324540gaccacattc tctgctgcct ctgccagttt gttctgcaat tttttgcaca cagacttact 324600ggctaattgc caatactatt accttgctct gttccaagcg ctctaaagct tctctgtaag 324660acagtggcaa cgaggacatg gactgtccaa gaactgtctc cattttgctg aggttagtgt 324720aaatatcctc ttccattttt ctatagcatt tgtaattctc aagataccta gtgagtcaca 324780gttagaaaaa aagtggtatt aaattttatt taacacaata aattcaaaat ttcccagaat 324840aaatattcca cacttaattt ttaagaaaag atatgacaac tttcgttttt gctaggcaat 324900gcattttaaa atgttttcca agaagttttt attcccaaag ggacctgcat atgggtttaa 324960atgcaaactt gaaaaggaag caagggccgt cctctaaaat gaagattttt tttttttaaa 325020gttcacttca gtaaaatgaa aactgcattc tcaaacacac tgatgagctg taaattggta 325080caattcttgt ggaaaaccac ttagtcttaa aactattccc atctttgacc cattaatgca 325140gcttctgcaa tttaccttaa gaactgtatc ctaaacatat agagcttatc ctaaatcttt 325200tgcacagtaa aacatcttta ctcactagac tattaattaa aacatcacac attggaatac 325260ccttatctaa caaaggtatg attaagaaat taaaacaatt catcgtatac aatattatag 325320agtaattaca ttacttttag aaagtatttg ttacaatcaa gaaaagaact gttataaaat 325380tttaaaataa agctggatat ttgatatttt atatatagca tatatatttt aaaacactaa 325440aattatgcat acaaaaacag acacagccta aatcccccat tgaatgtaac taaaaactct 325500atatcaaatt attcttaaaa aacaactctt aaaacgcatg aataaattga caaggaatta 325560gggagtcaaa ctgtaaaatg gaaatataca gaaataggca aagttgtagg accagttttt 325620tgccttgagg gcacttgagt ttcaattttc tttgattcct actgaaaggt gggggacaga 325680ccataaagct gggagccaat cccaggatgg gtgtctaata gaaaactccc tctgcaagaa 325740tatttgtaac acatataact ataaaggacc actctctaaa atatagaatt c 325791 2 2011 DNA Human 2tttcagtttc tccagctgct ggctttttgg acacccactc ccccgccagg aggcagttgc 60aagcgcggag gctgcgagaa ataactgcct cttgaaactt gcagggcgaa gagcaggcgg 120cgagcgctgg gccggggagg gaccacccga gctgcgacgg gctctggggc tgcggggcag 180ggctggcgcc cggagcctga gctgcaggag gtgcgctcgc tttcctcaac aggtggcggc 240ggggcgcgcg ccgggagacc ccccctaatg cgggaaaagc acgtgtccgc attttagaga 300aggcaaggcc ggtgtgttta tctgcaagcc attatacttg cccacgaatc tttgagaaca 360ttataatgac ctttgtgcct cttcttgcaa ggtgttttct cagctgttat ctcaagacat 420ggatataaaa aactcaccat ctagccttaa ttctccttcc tcctacaact gcagtcaatc 480catcttaccc ctggagcacg gctccatata cataccttcc tcctatgtag acagccacca 540tgaatatcca gccatgacat tctatagccc tgctgtgatg aattacagca ttcccagcaa 600tgtcactaac ttggaaggtg ggcctggtcg gcagaccaca agcccaaatg tgttgtggcc 660aacacctggg cacctttctc ctttagtggt ccatcgccag ttatcacatc tgtatgcgga 720acctcaaaag agtccctggt gtgaagcaag atcgctagaa cacaccttac ctgtaaacag 780agagacactg aaaaggaagg ttagtgggaa ccgttgcgcc agccctgtta ctggtccagg 840ttcaaagagg gatgctcact tctgcgctgt ctgcagcgat tacgcatcgg gatatcacta 900tggagtctgg tcgtgtgaag gatgtaaggc cttttttaaa agaagcattc aaggacataa 960tgattatatt tgtccagcta caaatcagtg tacaatcgat aaaaaccggc gcaagagctg 1020ccaggcctgc cgacttcgga agtgttacga agtgggaatg gtgaagtgtg gctcccggag 1080agagagatgt gggtaccgcc ttgtgcggag acagagaagt gccgacgagc agctgcactg 1140tgccggcaag gccaagagaa gtggcggcca cgcgccccga gtgcgggagc tgctgctgga 1200cgccctgagc cccgagcagc tagtgctcac cctcctggag gctgagccgc cccatgtgct 1260gatcagccgc cccagtgcgc ccttcaccga ggcctccatg atgatgtccc tgaccaagtt 1320ggccgacaag gagttggtac acatgatcag ctgggccaag aagattcccg gctttgtgga 1380gctcagcctg ttcgaccaag tgcggctctt ggagagctgt tggatggagg tgttaatgat 1440ggggctgatg tggcgctcaa ttgaccaccc cggcaagctc atctttgctc cagatcttgt 1500tctggacagg gatgagggga aatgcgtaga aggaattctg gaaatctttg acatgctcct 1560ggcaactact tcaaggtttc gagagttaaa actccaacac aaagaatatc tctgtgtcaa 1620ggccatgatc ctgctcaatt ccagtatgta ccctctggtc acagcgaccc aggatgctga 1680cagcagccgg aagctggctc acttgctgaa cgccgtgacc gatgctttgg tttgggtgat 1740tgccaagagc ggcatctcct cccagcagca atccatgcgc ctggctaacc tcctgatgct 1800cctgtcccac gtcaggcatg cgagtaacaa gggcatggaa catctgctca acatgaagtg 1860caaaaatgtg gtcccagtgt atgacctgct gctggagatg ctgaatgccc acgtgcttcg 1920cgggtgcaag tcctccatca cggggtccga gtgcagcccg gcagaggaca gtaaaagcaa 1980agagggctcc cagaacccac agtctcagtg a 2011 3 530 PRT Human 3Met Asp Ile Lys Asn Ser Pro Ser Ser Leu Asn Ser Pro Ser Ser Tyr 1 5 10 15Asn Cys Ser Gln Ser Ile Leu Pro Leu Glu His Gly Ser Ile Tyr Ile 20 25 30Pro Ser Ser Tyr Val Asp Ser His His Glu Tyr Pro Ala Met Thr Phe 35 40 45Tyr Ser Pro Ala Val Met Asn Tyr Ser Ile Pro Ser Asn Val Thr Asn 50 55 60Leu Glu Gly Gly Pro Gly Arg Gln Thr Thr Ser Pro Asn Val Leu Trp65 70 75 80Pro Thr Pro Gly His Leu Ser Pro Leu Val Val His Arg Gln Leu Ser 85 90 95His Leu Tyr Ala Glu Pro Gln Lys Ser Pro Trp Cys Glu Ala Arg Ser 100 105 110Leu Glu His Thr Leu Pro Val Asn Arg Glu Thr Leu Lys Arg Lys Val 115 120 125Ser Gly Asn Arg Cys Ala Ser Pro Val Thr Gly Pro Gly Ser Lys Arg 130 135 140Asp Ala His Phe Cys Ala Val Cys Ser Asp Tyr Ala Ser Gly Tyr His145 150 155 160Tyr Gly Val Trp Ser Cys Glu Gly Cys Lys Ala Phe Phe Lys Arg Ser 165 170 175Ile Gln Gly His Asn Asp Tyr Ile Cys Pro Ala Thr Asn Gln Cys Thr 180 185 190Ile Asp Lys Asn Arg Arg Lys Ser Cys Gln Ala Cys Arg Leu Arg Lys 195 200 205Cys Tyr Glu Val Gly Met Val Lys Cys Gly Ser Arg Arg Glu Arg Cys 210 215 220Gly Tyr Arg Leu Val Arg Arg Gln Arg Ser Ala Asp Glu Gln Leu His225 230 235 240Cys Ala Gly Lys Ala Lys Arg Ser Gly Gly His Ala Pro Arg Val Arg 245 250 255Glu Leu Leu Leu Asp Ala Leu Ser Pro Glu Gln Leu Val Leu Thr Leu 260 265 270Leu Glu Ala Glu Pro Pro His Val Leu Ile Ser Arg Pro Ser Ala Pro 275 280 285Phe Thr Glu Ala Ser Met Met Met Ser Leu Thr Lys Leu Ala Asp Lys 290 295 300Glu Leu Val His Met Ile Ser Trp Ala Lys Lys Ile Pro Gly Phe Val305 310 315 320Glu Leu Ser Leu Phe Asp Gln Val Arg Leu Leu Glu Ser Cys Trp Met 325 330 335Glu Val Leu Met Met Gly Leu Met Trp Arg Ser Ile Asp His Pro Gly 340 345 350Lys Leu Ile Phe Ala Pro Asp Leu Val Leu Asp Arg Asp Glu Gly Lys 355 360 365Cys Val Glu Gly Ile Leu Glu Ile Phe Asp Met Leu Leu Ala Thr Thr 370 375 380Ser Arg Phe Arg Glu Leu Lys Leu Gln His Lys Glu Tyr Leu Cys Val385 390 395 400Lys Ala Met Ile Leu Leu Asn Ser Ser Met Tyr Pro Leu Val Thr Ala 405 410 415Thr Gln Asp Ala Asp Ser Ser Arg Lys Leu Ala His Leu Leu Asn Ala 420 425 430Val Thr Asp Ala Leu Val Trp Val Ile Ala Lys Ser Gly Ile Ser Ser 435 440 445Gln Gln Gln Ser Met Arg Leu Ala Asn Leu Leu Met Leu Leu Ser His 450 455 460Val Arg His Ala Ser Asn Lys Gly Met Glu His Leu Leu Asn Met Lys465 470 475 480Cys Lys Asn Val Val Pro Val Tyr Asp Leu Leu Leu Glu Met Leu Asn 485 490 495Ala His Val Leu Arg Gly Cys Lys Ser Ser Ile Thr Gly Ser Glu Cys 500 505 510Ser Pro Ala Glu Asp Ser Lys Ser Lys Glu Gly Ser Gln Asn Pro Gln 515 520 525Ser Gln 530

Claims

1. An isolated nucleic acid molecule comprising SEQ ID NO:1, wherein position 89837 of SEQ ID NO:1 is ‘T’ instead of ‘C’.

2. A nucleic acid probe that is complementary over the entire length of said probe to a segment of SEQ ID NO:1 that includes position 89837 of SEQ ID NO: 1, wherein position 89837 of SEQ ID NO: 1 is ‘T’ instead of ‘C’, such that the probe hybridizes under high stringency conditions to a nucleic acid molecule comprising said segment of SEQ ID NO: 1 but does not hybridize to a nucleic acid molecule comprising said segment of SEQ ID NO: 1 having a ‘C’ at position 89837, wherein said high stringency conditions are hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50-65° C.

3. The probe of claim 2, wherein the probe is detectably labeled.

4. An isolated nucleic acid molecule that is entirely complementary to the nucleic acid molecule of claim 1.