Claims
- 1. A dicot plant comprising in its genome a copy of a gene construct comprising, in sequence 5' to 3':
- a promoter sequence that initiates transcription in plant cells;
- a translational enhancer sequence from the transcribed but untranslated sequence immediately preceeding the coding region of the alfalfa mosaic virus coat protein gene;
- a protein coding sequence encoding a protein of less than about 700 amino acids, the protein being a Bacillus thuringiensis delta-endotoxin which is toxic upon ingestion to Manduca sexta; and
- a polyadenylation sequence, the gene construct effectively expressing in the cells of the plant a protein toxic to Manduca sexta.
- 2. Seed of the plant of claim 1.
- 3. The plant as claimed in claim 1 wherein the translational enhancer sequence consists of nucleotide positions 6 through 30 of SEQ ID NO:5.
- 4. The plant as claimed in claim 1 wherein the protein coding sequence is the amino-terminal 644 codons of the sequence of the delta-endotoxin of Bacillus thuringiensis var. kurstaki HD-1-Dipel.
- 5. The plant as claimed in claim 1 wherein the gene construct further comprises, between the protein coding sequence and the polyadenylation sequence, a sequence coding for two proline amino acids.
- 6. The plant as claimed in claim 1 wherein the gene construct comprises nucleotides number 16 to 2723 of plasmid pAMVBTS, ATCC Accession No. 53637.
CROSS-REFERENCE TO RELATED APPLICATION
This is a continuation, of application Ser. No. 07/299,767 filed Jan. 23, 1989 issued as U.S. Pat. No. 5,608,142, which is a continuation-in-part of application Ser. No. 07/123,054 filed Nov 19, 1987, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5608142 |
Barton et al. |
Mar 1997 |
|
Non-Patent Literature Citations (1)
Entry |
Koziel MG, et al. "Optimizing expression of transgenes with an emphasis on post-transcriptional events." Plant Mol. Biol. 32: 393-405, 1996. |
Continuations (1)
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Number |
Date |
Country |
Parent |
299767 |
Jan 1989 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
123054 |
Nov 1987 |
|