Claims
- 1. A method for the expression of a somatotropin in plants said method comprising:
(a) introducing into a plant cell a chimeric nucleic acid sequence comprising:
(1) a first nucleic acid sequence capable of regulating the transcription in said host cell of (2) a second nucleic acid sequence, wherein said second sequence encodes a recombinant fusion polypeptide and comprises (i) a nucleic acid sequence encoding a sufficient portion of an oleosin protein to provide targeting of the recombinant fusion polypeptide to a lipid phase, linked in frame to (ii) a nucleic acid sequence encoding said somatotropin; and (3) a third DNA sequence encoding a termination region functional in said plant cell; and (b) growing said plant cell to produce said recombinant fusion polypeptide.
- 2. The method according to claim 1 further including separating the recombinant fusion polypeptide from cellular host cell components by selective partitioning into a lipid phase.
- 3. The method according to claim 1 further including separating the recombinant fusion polypeptide from cellular host components by selective partitioning into a lipid phase comprising oil bodies.
- 4. The method according to claim 3 wherein said recombinant fusion polypeptide is separated by addition of oil body components and reconstitution of the oil bodies.
- 5. A method according to claim 1 wherein said somatotropin is a fish growth hormone.
- 6. A method according to claim 5 wherein said fish growth hormone is carp growth hormone.
- 7. A chimeric nucleic acid sequence encoding a recombinant fusion polypeptide comprising (i) a nucleic acid sequence encoding a sufficient portion of an oleosin protein to provide targeting of the recombinant fusion polypeptide to a lipid phase, linked in reading frame to (ii) a nucleic acid sequence encoding a somatotropin.
- 8. A chimeric nucleic acid sequence according to claim 7 having the nucleic acid sequence shown in SEQ.ID.NO.:1.
- 9. A chimeric nucleic acid sequence, capable of being expressed in association with an oil body of a plant cell, comprising:
(1) a first nucleic acid sequence capable of regulating the transcription in said plant cell (2) a second nucleic acid sequence, wherein said second sequence encodes a recombinant fusion polypeptide and comprises (i) a nucleic acid sequence encoding a sufficient portion of an oleosin protein to provide targeting of the recombinant fusion polypeptide to a lipid phase, linked in reading frame to (ii) a nucleic acid sequence encoding a somatotropin; and (3) a third nucleic acid sequence encoding a termination region functional in said host cell.
- 10. A chimeric nucleic acid sequence according to claim 9 wherein said somatotropin is a fish growth hormone.
- 11. A chimeric nucleic acid sequence according to claim 10 wherein said fish growth hormone is carp growth hormone.
- 12. A plant transformed with a chimeric nucleic acid sequence according to claim 9.
- 13. A plant according to claim 10 wherein said plant is selected from the group comprising rapeseed (Brassica spp.), linseed/flax (Linum usitatissimum), safflower (Carthamus tinctorius), sunflower (Helianthus annuus), maize (Zea mays), soybean (Glycine max), mustard (Brassica spp. and Sinapis alba), crambe, (Crambe abyssinica), eruca (Eruca sativa), oil palm (Elaeis guineeis), cottonseed (Gossypium spp.), groundnut (Arachis hypogaea), coconut (Cocus nucifera), castor bean (Ricinus communis), coriander (Coriandrum sativum), squash, (Cucurbita maxima), Brazil nut (Bertholletia excelsa) and jojoba (Simmondsia chinensis).
- 14. A plant seed containing a chimeric nucleic acid sequence according to claim 9.
- 15. A plant seed according to claim 14 wherein said seed is obtained from a dicotelydenous plant.
- 16. A plant seed according to claim 14 wherein said somatotropin is expressed in the embryogenic tissue of the seed.
- 17. A plant seed comprising a recombinantly expressed somatotropin.
- 18. A plant seed according to claim 17 wherein said somatotropin is expressed as a fusion protein.
- 19. A plant seed according to claim 18 wherein said fusion protein comprises an oleosin.
- 20. A plant seed according to claim 17 wherein said somatotropin is expressed in the embryogenic tissue of said seed.
- 21. A plant seed according to claim 19 wherein said somatotropin is expressed in the embryogenic tissue of said seed.
- 22. A plant seed according to claim 21 wherein said somatotropin is fish growth hormone.
- 23. A plant seed according to claim 18 wherein said plant seed is obtained from a dicotelydenous plant.
- 24. A plant seed according to claim 18 wherein said seed is exalbuminous seed.
- 25. A plant seed according to claim 18 wherein said plant seed is obtained from the group of plants comprising rapeseed (Brassica spp.), linseed/flax (Linum usitatissimum), safflower (Carthamus tinctorius), sunflower (Helianthus annuus), maize (Zea mays), soybean (Glycine max), mustard (Brassica spp. and Sinapis alba), crambe, (Crambe abyssinica), eruca (Eruca sativa), oil palm (Elaeis guineeis), cottonseed (Gossypium spp.), groundnut (Arachis hypogaea), coconut (Cocus nucifera), castor bean (Ricinus communis), coriander (Coriandrum sativum), squash, (Cucurbita maxima), Brazil nut (Bertholletia excelsa) and jojoba (Simmondsia chinensis).
- 26. A fusion polypeptide encoded for by a chimeric nucleic acid sequence comprising (i) a nucleic acid sequence encoding a sufficient portion of an oil body protein to provide targeting of the fusion polypeptide to an oil body linked in reading frame to (ii) a nucleic acid sequence encoding a somatotropin.
- 27. A fusion polypeptide comprising a somatotropin linked to an oil body protein.
- 28. A fusion polypeptide according to claim 27 having the sequence shown in SEQ.ID.NO.:2.
- 29. A fusion polypeptide according to claim 27 wherein said somatotropin is biologically active.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation-in-part of U.S. Ser. No. 08/846,021 that was filed on Apr. 25, 1997 which is a continuation-in-part of U.S. Ser. No. 08/366,783 that was filed on Dec. 30, 1994 which is a continuation-in-part of U.S. Ser. No. 08/142,418 that was filed Nov. 16, 1993 which is a continuation-in-part of U.S. Ser. No. 07/659,835 that was filed on Feb. 22, 1991, all of which are incorporated herein by reference.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09210843 |
Dec 1998 |
US |
Child |
09887569 |
Jun 2001 |
US |
Continuation in Parts (4)
|
Number |
Date |
Country |
Parent |
08846021 |
Apr 1997 |
US |
Child |
09210843 |
Dec 1998 |
US |
Parent |
08366783 |
Dec 1994 |
US |
Child |
08846021 |
Apr 1997 |
US |
Parent |
08142418 |
Nov 1993 |
US |
Child |
08366783 |
Dec 1994 |
US |
Parent |
07659835 |
Feb 1991 |
US |
Child |
08142418 |
Nov 1993 |
US |