Claims
- 1. An expression vector comprising an isolated nucleic acid sequence comprising: (a) the sequence as shown in SEQ ID NO: 1; (b) the complement of (a); or (c) a fragment of SEQ ID NO: 1, wherein said nucleic acid sequence is capable of increasing the expression of a CTLA4 variant.
- 2. The expression vector of claim 1 further comprising a selectable marker.
- 3. The expression vector of claim 1 comprising nucleotides 3059 through 4709 of SEQ ID NO: 1.
- 4. The expression vector of claim 1 wherein the CTLA4 variant is LEA29YIg.
- 5. The expression vector of claim 1, further comprising a nucleic acid sequence encoding all or a portion of a CTLA4 variant.
- 6. The expression vector of claim 5, wherein the CTLA4 variant is LEA29YIg.
- 7. The expression vector of claim 3, further comprising a nucleic acid sequence encoding all or a portion of a CTLA4 variant.
- 8. The expression vector of claim 7, wherein the CTLA4 variant is LEA29YIg.
- 9. A host cell transformed with the expression vector of claim 1.
- 10. A host cell transformed with the expression vector of claim 2.
- 11. A host cell transformed with the expression vector of claim 3.
- 12. A host cell transformed with the expression vector of claim 5.
- 13. A host cell transformed with the expression vector of claim 6.
- 14. A host cell transformed with the expression vector of claim 7.
- 15. A CHO cell transformed with the expression vector of claim 1.
- 16. A CHO cell transformed with the expression vector of claim 2.
- 17. A CHO cell transformed with the expression vector of claim 3.
- 18. A CHO cell transformed with the expression vector of claim 5.
- 19. A CHO cell transformed with the expression vector of claim 6.
- 20. A CHO cell transformed with the expression vector of claim 7.
- 21. An expression vector comprising an isolated nucleic acid sequence comprising: (a) the sequence as shown in SEQ ID NO: 1; (b) the complement of (a); or (c) a fragment of SEQ ID NO: 1 wherein said nucleic acid sequence is capable of increasing the expression of a recombinant protein of interest wherein said recombinant protein of interest is an antibody.
- 22. An expression vector of claim 21 further comprising two nucleic acid sequences encoding different polypeptides in the same vector which assemble into a protein of interest with biological activity.
- 23. A host cell transformed with two expression vectors wherein each of the expression vectors comprises an isolated nucleic acid sequence capable of increasing the expression of a recombinant protein of interest said nucleic acid sequence comprising: (a) the sequence as shown in SEQ ID NO: 1; (b) the complement of (a); or (c) a fragment of SEQ ID NO: 1, and wherein each of the two expression vectors encodes a polypeptide subunit that when coexpressed in the host cell assembles into a protein of interest with biological activity.
- 24. The host cell of claim 23 wherein the protein of interest is anti-CD40 antibody.
- 25. A method for obtaining a recombinant CTLA4-Ig variant comprising culturing a host cell according to claim 12 under conditions promoting expression of the CTLA4-Ig variant, and recovering the CTLA4-Ig variant.
- 26. A method for obtaining a recombinant LEA29YIg, comprising culturing a host cell according to claim 13 under conditions promoting expression of said LEA29YIg and recovering the LEA29YIg.
- 27. A method for obtaining a recombinant biologically active anti-CD40 antibody, comprising culturing a host cell according to claim 24 under conditions promoting expression of said anti-CD40 antibody, and recovering the anti-CD40 antibody.
Parent Case Info
[0001] This application is a continuation-in-part application of U.S. patent application Ser. No. 09/386,816, filed Aug. 31, 1999, which claims the benefit of U.S. Provisional Application Serial No. 60/101,292, filed Sep. 22, 1998, the contents of all of which are incorporated by reference herein in their entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60101292 |
Sep 1998 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09386816 |
Aug 1999 |
US |
Child |
10320176 |
Dec 2002 |
US |