Claims
- 1. An isolated and purified nucleic acid molecule coding for a protein capable of forming a potassium (K+) ion channel, comprising two P domains and three or four transmembrane segments.
- 2. The nucleic acid molecule of claim 1 coding for a protein wherein the number of P domains is two and the number of transmembrane segments is four.
- 3. The nucleic acid molecule of claim 1 which is human.
- 4. The nucleic acid molecule of claim 1 which is a cDNA copy of a 2.6 kilobase transcript expressed at high levels in the pancreas and placenta, and at lower levels in the brain, lung, prostate, heart, kidney, uterus small intestine and colon.
- 5. The nucleic acid sequence of claim 1 which codes for a protein which comprises the sequence represented by SEQ ID NO:4.
- 6. The isolated and purified nucleic acid sequence of claim 1 which codes for a protein which comprises the sequence represented by SEQ ID NO:4 or a sequence having the equivalent function of being capable of forming a potassium (K+) ion channel which comprises two P domains and four transmembrane segments.
- 7. An isolated and purified nucleic acid sequence of claim 2 which comprises an open reading frame (ORF) of 1185 nucleotides.
- 8. The isolated and purified nucleic acid sequence of claim 7 which is human.
- 9. A self replicating vector comprising the nucleic acid molecule of claim 1.
- 10. A cell transformed with the vector of claim 9, which cell is selected from the group consisting of prokaryotes and eukaryotes.
- 11. The transformed cell of claim 10 which is a yeast, insect cell, plant cell or mammalian cell.
- 12. The transformed cell of claim 10 which is a bacterium.
- 13. A method for the expression and isolation of a potassium transport channel encoded by a nucleic acid molecule according to claim 1 in a competent host cell comprising transferring a self-replicating vector including said nucleic acid molecule into a competent host cell, culturing said host cell under conditions allowing the production of the potassium transport channel, and isolating and purifying the polypeptide comprising the potassium transport channel.
Priority Claims (1)
Number |
Date |
Country |
Kind |
96 01565 |
Feb 1996 |
FR |
|
RELATED APPLICATIONS
This patent application is a continuation-in-part (CIP) of pending application Ser. No. 08/749,816 Lesage et al., filed in the U.S. on Nov. 15, 1996, now U.S. Pat. No. 6,013,476 entitled, “New Family of Mammalian Potassium Channels, Their Cloning And Their Use, Especially For The Screening of Drugs” which is incorporated herein by reference in its entirety, which claims the priority filing date of French patent application Ser. No. 96/01565, filed Feb. 8, 1996.
This application also claims the priority of provisional patent application Ser. No. 60/095,234, filed on Aug. 4, 1998, entitled “Task, a Human Background K+channel to Sense External PH Variations near Physiological PH” which is also incorporated herein by reference in its entirety.
Non-Patent Literature Citations (3)
Entry |
Kubo et al. Nature. vol. 362, 127-131, 1993.* |
Lesage et al. EMBO J. vol. 15(5), 1004-1011, Mar. 1, 1996.* |
Duprat et al. EMBO J. vol. 16(17), 5464-5471, Sep. 1, 1997. |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/095234 |
Aug 1998 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08/749816 |
Nov 1996 |
US |
Child |
09/144914 |
|
US |