Feline polynucleotide vaccine formula

Abstract

Disclosed are plasmids that contain and express in vivo in a feline host cell nucleic acid molecules. The plasmid can include nucleic molecule(s) having sequence(s) encoding infectious peritonitis virus M; feline immunodeficiency virus env, or gag, or pro, or gag and pro, or env and gag and pro; rabies G; or feline leukemia virus env and/or gag. Compositions containing such plasmids, methods of use employing such plasmids, and kits involving such plasmids, are also disclosed.

Description

The present invention relates to a vaccine formula allowing the vaccination of cats against a number of pathologies. It also relates to a corresponding method of vaccination.

Associations of vaccines against certain canine viruses have already been proposed in the past.

The associations developed so far were prepared from inactivated vaccines or live vaccines and, optionally, mixtures of such vaccines. Their development poses problems of compatibility between valencies and of stability. It is indeed necessary to ensure both the compatibility between the different vaccine valencies, whether from the point of view of the different antigens used or from the point of view of the formulations themselves, especially in the case where both inactivated vaccines and live vaccines are combined. The problem of the conservation of such combined vaccines and of their safety especially in the presence of an adjuvant also exists. These vaccines are in general quite expensive.

Patent Applications WO-A-90 11092, WO-A-93 19183, WO-A-94 21797 and WO-A-95 20660 have made use of the recently developed technique of polynucleotide vaccines. It is known that these vaccines use a plasmid capable of expressing, in the host cells, the antigen inserted into the plasmid. All the routes of administration have been proposed (intraperitoneal, intravenous, intramuscular, transcutaneous, intradermal, mucosal and the like). Various vaccination means can also be used, such as DNA deposited at the surface of gold particles and projected so as to penetrate into the animals' skin (Tang et al., Nature 356, 152-154, 1992) and liquid jet injectors which make it possible to transfect at the same time the skin, the muscle, the fatty tissues and the mammary tissues (Furth et al., Analytical Biochemistry, 205, 365-368, 1992).

(See also U.S. Pat. Nos. 846,946, 5,620,896, 5,643,578, 5,580,589, 5,589,466, 5,693,622, and 5,703,055; Science, 259:1745-49, 1993; Robinson et al., seminars in IMMUNOLOGY, 9:271-83, 1997; Luke et al., J. Infect. Dis. 175(1):91-97, 1997; Norman et al., Vaccine, 15(8):801-803, 1997; Bourne et al., The Journal of Infectious Disease, 173:800-7, 1996; and, note that generally a plasmid for a vaccine or immunological composition can comprise DNA encoding an antigen operatively linked to regulatory sequences which control expression or expression and secretion of the antigen from a host cell, e.g., a mammalian cell; for instance, from upstream to downstream, DNA for a promoter, DNA for a eukaryotic leader peptide for secretion, DNA for the antigen, and DNA encoding a terminator.)

The polynucleotide vaccines may also use both naked DNAs and DNAs formulated, for example, inside cationic lipids or liposomes.

The invention therefore proposes to provide a muitivalent vaccine formula which makes it possible to ensure vaccination against a number of feline pathogenic viruses.

Another objective of the invention is to provide such a vaccine formula combining different valencies while exhibiting all the criteria required for mutual compatibility and stability of the valencies.

Another objective of the invention is to provide such a vaccine formula which makes it possible to combine different valencies in the same vehicle.

Another objective of the invention is to provide such a vaccine which is easy and inexpensive to use.

Yet another objective of the invention is to provide a method for vaccinating cats which makes it possible to obtain protection, including multivalent protection, with a high level of efficiency and of long duration, as well as good safety.

The subject of the present invention is therefore a vaccine formula intended for cats, comprising at least three polynucleotide vaccine valencies each comprising a plasmid integrating, so as to express it in vivo in the host cells, a gene with one feline pathogen valency, these valencies being selected from those of the group consisting of feline leukaemia virus (FeLV), panleukopenia virus (FPV), infectious peritonitis virus (FIPV), coryza virus (FHV), calicivirosis virus (FCV), feline immunodeficiency virus (FIV) and possibly rabies virus (rhabdovirus), the plasmids comprising, for each valency, one or more of the genes selected from the group consisting of env and gag/pol for the feline leukaemia, VP2 for the panleukopenia, modified S (or S*) and M for the infectious peritonitis, gB and gD for the coryza, capsid for the calicivirosis, env and gag/pro for the feline immunodeficiency and G for the rabies.

Valency in the present invention is understood to mean at least one antigen providing protection against the virus for the pathogen considered, it being possible for the valency to contain, as subvalency, one or more modified or natural genes from one or more strains of the pathogen considered.

Pathogenic agent gene is understood to mean not only the complete gene but also the various nucleotide sequences, including fragments which retain the capacity to induce a protective response. The notion of a gene covers the nucleotide sequences equivalent to those described precisely in the examples, that is to say the sequences which are different but which encode the same protein. It also covers the nucleotide sequences of other strains of the pathogen considered, which provide cross-protection or a protection specific for a strain or for a strain group. It also covers the nucleotide sequences which have been modified in order to facilitate the in vivo expression by the host animal but encoding the same protein.

Preferably, the vaccine formula according to the invention comprises the panleukopenia, coryza and calicivirosis valencies.

It will be possible to add the feline leukaemia, feline immunodeficiency and/or infectious peritonitis valencies.

As regards the coryza valency, it is preferable to use the two genes coding for gB and gD, in different plasmids or in one and the same plasmid, or to use either of these genes.

For the feline leukaemia valency, use is preferably made of the two env and gag/pol genes integrated into two different plasmids or into one and the same plasmid, or the env gene alone.

For the feline immunodeficiency valency, use will preferably be made of the two env and gag/pro genes in different plasmids or in one and the same plasmid, or only one of these genes. Still more preferably, the FeLV-A env gene and the FeLV-A and FeLV-B env genes are used.

For the infectious peritonitis valency, use is preferably made of the two M and modified S genes together in two different plasmids or in one and the same plasmid, or either of these genes. S will be modified in order to make the major facilitating epitopes inactive, preferably according to the teaching of Patent PCT/FR95/01128.

The vaccine formula according to the invention can be presented in a dose volume of between 0.1 and 3 ml and in particular between 0.5 and 1 ml.

The dose will be generally between 10 ng and 1 mg, preferably between 100 ng and 500 μg and still more preferably between 1 μg and 250 μg per plasmid type.

Use will preferably be made of naked plasmids simply placed in the vaccination vehicle which will be in general physiological saline (0.9% NaCl), ultrapure water, TE buffer and the like. All the polynucleotide vaccine forms described in the prior art can of course be used.

Each plasmid comprises a promoter capable of ensuring the expression of the gene inserted, under its control, into the host cells. This will be in general a strong eukaryotic promoter and in particular a cytomegalovirus early CMV-IE promoter of human or murine origin, or optionally of another origin such as rats, pigs and guinea pigs.

More generally, the promoter may be either of viral origin or of cellular origin. As viral promoter, there may be mentioned the SV40 virus early or late promoter or the Rous sarcoma virus LTR promoter. It may also be a promoter from the virus from which the gene is derived, for example the gene's own promoter.

As cellular promoter, there may be mentioned the promoter of a cytoskeleton gene, such as for example the desmin promoter (Bolmont et al., Journal of Submicroscopic Cytology and Pathology, 1990, 22, 117-122; and Zhenlin et al., Gene, 1989, 78, 243-254), or alternatively the actin promoter.

When several genes are present in the same plasmid, these may be presented in the same transcription unit or in two different units.

The combination of the different vaccine valencies according to the invention may be preferably achieved by mixing the polynucleotide plasmids expressing the antigen(s) of each valency, but it is also possible to envisage causing antigens of several valencies to be expressed by the same plasmid.

The subject of the invention is also monovalent vaccine formulae comprising one or more plasmids encoding one or more genes from one of the viruses above, the genes being those described above. Besides their monovalent character, these formulae may possess the characteristics stated above as regards the choice of the genes, their combinations, the composition of the plasmids, the dose volumes, the doses and the like.

The monovalent vaccine formulae may also be used (i) for the preparation of a polyvalent vaccine formula as described above, (ii) individually against the actual pathology, (iii) combined with a vaccine of another type (live or inactivated whole, recombinant, subunit) against another pathology, or (iv) as booster for a vaccine as described below.

The subject of the present invention is in fact also the use of one or more plasmids according to the invention for the manufacture of a vaccine intended to vaccinate cats first vaccinated by means of a first conventional vaccine (monovalent or multivalent) of the type in the prior art, in particular, selected from the group consisting of a live whole vaccine, an inactivated whole vaccine, a subunit vaccine, a recombinant vaccine, this first vaccine having (that is to say containing or capable of expressing) the antigen(s) encoded by the plasmid(s) or antigen(s) providing cross-protection.

Remarkably, the polynucleotide vaccine has a potent booster effect which results in an amplification of the immune response and the acquisition of a long-lasting immunity.

In general, the first-vaccination vaccines can be selected from commercial vaccines available from various veterinary vaccine producers.

The subject of the invention is also a vaccination kit grouping together a first-vaccination vaccine as described above and a vaccine formula according to the invention for the booster. It also relates to a vaccine formula according to the invention accompanied by a leaflet indicating the use of this formula as a booster for a first vaccination as described above.

The subject of the present invention is also a method for vaccinating cats, comprising the administration of an effective vaccine formula as described above. This vaccination method comprises the administration of one or more doses of the vaccine formula, it being possible for these doses to be administered in succession over a short period of time and/or in succession at widely spaced intervals.

The vaccine formulae according to the invention can be administered in the context of this method of vaccination, by the different routes of administration proposed in the prior art for polynucleotide vaccination and by means of known techniques of administration.

The subject of the invention is also the method of vaccination consisting in making a first vaccination as described above and a booster with a vaccine formula according to the invention.

In a preferred embodiment of the process according to the invention, there is administered in a first instance, to the animal, an effective dose of the vaccine of the conventional, especially inactivated, live, attenuated or recombinant, type, or alternatively a subunit vaccine, so as to provide a first vaccination, and, after a period preferably of 2 to 6 weeks, the polyvalent or monovalent vaccine according to the invention is administered.

The efficiency of presentation of the antigens to the immune system varies according to the tissues. In particular, the mucous membranes of the respiratory tree serve as barrier to the entry of pathogens and are associated with lymphoid tissues which support local immunity. The administration of a vaccine by contact with the mucous membranes, in particular the buccal mucous membrane, the pharyngeal mucous membrane and the mucous membrane of the bronchial region is certainly of interest for vaccination against respiratory and digestive pathologies.

Consequently, the mucosal routes of administration form part of a preferred mode of administration for the invention, using in particular nebulization or spray or drinking water. It will be possible to apply the vaccine formulae and the vaccination methods according to the invention in this context.

The invention also relates to the method of preparing the vaccine formulae, namely the preparation of the valencies and mixtures thereof, as evident from this description.

The invention will now be described in greater detail with the aid of the embodiments of the invention taken with reference to the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. No. 1 : Plasmid pVR1012

FIG. No. 2 : Plasmid pPB179

FIG. No. 3 : Sequence of the FOLV-B env gone

FIG. No. 4 : Plasmid pPB180

FIG. No. 5 : Sequence of the FeLV-A virus gag/pol gene (Glasgow-1 strain)

FIG. No. 6 : Plasmid pPB181

FIG. No. 7 : Plasmid pAB009

FIG. No. 8 : Plasmid pAB053

FIG. No. 9 : Plasmid pAB052

FIG. No. 10 : Plasmid pAB056

FIG. No. 11 : Plasmid pAB028

FIG. No. 12 : Plasmid pAB029

FIG. No. 13 : Plasmid pAB010

FIG. No. 14 : Plasmid pAB030

FIG. No. 15 : Plasmid pAB083

FIG. No. 16 : Plasmid pAB041

Sequence listing SEQ ID No.

SEQ ID No. 1: Oligonucleotide PB247

SEQ ID No. 2: Oligonucleotide PB249

SEQ ID No. 3: Oligonucleotide PB281

SEQ ID No. 4: Oligonucleotide PB282

SEQ ID No. 5: Sequence of the FELV-B virus env gene

SEQ ID No. 6: Oligonucleotide PB283

SEQ ID No. 7: Oligonuclootide PB284

SEQ ID No. 8: Sequence of the FeLV-A virus gag/pol gene (Glasgow-1 strain)

SEQ ID No. 9: Oligonucleotide AB021

SEQ ID No. 10: Oligonucleotide AB024

SEQ ID No. 11: Oligonucleotide AB103

SEQ ID No. 12: Oligonucleotide AB112

SEQ ID No. 13: Oligonucleotide AB113

SEQ ID No. 14: Oligonucleotide AB104

SEQ ID No. 15: Oligonucleotide AB101

SEQ ID No. 16: Oligonucleotide AB102

SEQ ID No. 17: Oligonuclootide AB106

SEQ ID No. 18: Oligonucleotide AB107

SEQ ID No. 19: Oligonucleotide AB061

SEQ ID No. 20: Oligonucleotide AB064

SEQ ID No. 21: Oligonucleotide AB065

SEQ ID No. 22: Oligonuclootide AB066

SEQ ID No. 23: Oligonucleotide AB025

SEQ ID No. 24: Oligonucleotide AB026

SEQ ID No. 25: Oligonucleotide AB067

SEQ ID No. 26: Oligonucleotide AB070

SEQ ID No. 27: Oligonucleotide AB154

SEQ ID No. 28: Oligonucleotide AB155

SEQ ID No. 29: Oligonucleotide AB011

SEQ ID No. 30: Oligonucleotide AB012

EXAMPLES

Example 1

Culture of the Viruses

The viruses are cultured on the appropriate cellular system until a cytopathic effect is obtained. The cellular systems to be used for each virus are well known to persons skilled in the art. Briefly, the cells sensitive to the virus used, which are cultured in Eagle's minimum essential medium (MEM medium) or another appropriate medium, are inoculated with the viral strain studied using a multiplicity of infection of 1. The infected cells are then incubated at 37° C. for the time necessary for the appearance of a complete cytopathic effect (on average 36 hours).

Example 2

Extraction of the Viral Genomic DNAs

After culturing, the supernatant and the lysed cells are harvested and the entire viral suspension is centrifuged at 1000 g for 10 minutes at +4° C. so as to remove the cellular debris. The viral particles are then harvested by ultracentrifugation at 400,000 g for 1 hour at +4° C. The pellet is taken up in a minimum volume of buffer (10 mM Tris, 1 mM EDTA). This concentrated viral suspension is treated with proteinase K (100 μg/ml final) in the presence of sodium dodecyl sulphate (SDS) (0.5% final) for 2 hours at 37° C. The viral DNA is then extracted with a phenol/chloroform mixture and then precipitated with 2 volumes of absolute ethanol. After leaving overnight at −20° C., the DNA is centrifuged at 10,000 g for 15 minutes at +4° C. The DNA pellet is dried and then taken up in a minimum volume of sterile ultrapure water. It can then be digested with restriction enzymes.

Example 3

Isolation of the Viral Genamic RNAs

The RNA viruses were purified according to techniques well known to persons skilled in the art. The genomic viral RNA of each virus was then isolated using the “guanidium thiocyanate/phenol-chloroform” extraction technique described by P. Chomczynski and N. Sacchi (Anal. Biochem., 1987, 162, 156-159).

Example 4

Molecular Biology Techniques

All the constructions of plasmids were carried out using the standard molecular biology techniques described by J. Sambrook et al. ( Molecular Cloning: A Laboratory Manual , 2nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989). All the restriction fragments used for the present invention were isolated using the “Geneclean” kit (BIO 101 Inc. La Jolla, Calif.).

Example 5

RT-PCR Technique

Specific oligonucleotides (comprising restriction sites at their 5′ ends to facilitate the cloning of the amplified fragments) were synthesized such that they completely cover the coding regions of the genes which are to be amplified (see specific examples). The reverse transcription (RT) reaction and the polymerase chain reaction (PCR) were carried out according to standard techniques (Sambrook J. et al., 1989). Each RT-PCR reaction was performed with a pair of specific amplimers and taking, as template, the viral genomic RNA extracted. The complementary DNA amplified was extracted with phenol/chloroform/isoamyl alcohol (25:24:1) before being digested with restriction enzymes.

Example 6

Plasmid pVR1012

The plasmid pVR1012 (FIG. No. 1 ) was obtained from Vical Inc., San Diego, Calif., USA. Its construction has been described in J. Hartikka et al. (Human Gene Therapy, 1996, 7, 1205-1217).

Example 7

Construction of the Plasmid pPB179 (FeLV-A Virus Env Gene)

An RT-PCR reaction according to the technique of Example 5 was carried out with feline leukaemia virus (FeLV-A) (Glasgow-1 strain) genomic RNA (M. Stewart et al. J. Virol. 1986. 58. 825-834), prepared according to the technique of Example 3, and with the following oligonucleotides:

PB247 (29 mer) (SEQ ID No. 1)

5′TTTGTCGACCATGGAAAGTCCAACGCACC3′

PB249 (28 mer) (SEQ ID No. 2)

5′TTTGGATCCTCATGGTCGGTCCGGATCG3′

so as to amplify a 1947 bp fragment containing the gene encoding the Env glycoprotein from the FeLV-A virus (Glasgow-1 strain) in the form of a SalI-BamHI fragment. After purification, the RT-PCR product was digested with SalI and BamHI in order to give a 1935 bp SalI-BamHI fragment.

This fragment was ligated with the vector pVR1012 (Example 6), previously digested with SalI and BamHI, to give the plasmid pPB179 (6804 bp) (FIG. No. 2 ).

Example 8

Construction of the Plasmid pPB180 (FeLV-S Virus Env Gene)

An RT-PCR reaction according to the technique of Example 5 was carried out with feline leukaemia virus (FeLV-B subtype) genomic RNA, prepared according to the technique of Example 3, and with the following oligonucleotides:

PB281 (29 mer) (SEQ ID No. 3)

5′TTTGTCGACATGGAAGGTCCAACGCACCC3′

PB282 (32 mer) (SEQ ID No. 4)

5′TTGGATCCTCATGGTCGGTCCGGATCATATTG3′

so as to amplify a 2005 bp fragment containing the gene encoding the Env glycoprotein from the FeLV-B virus (FIG. No. 3 and SEQ ID No. 5) in the form of a SalI-BamHI fragment. After purification, the RT-PCR product was digested with SalI and BamHI in order to give a 1995 bp SalI-BamHI fragment.

This fragment was ligated with the vector pVR1012 (Example 6), previously digested with SalI and BamHI, to give the plasmid pPB180 (6863 bp) (FIG. No. 4 ).

Example 9

Construction of the Plasmid pPB181 (FeLV gag/pol gene)

An RT-PCR reaction according to the technique of Example 5 was carried out with the feline leukaemia virus (FeLV-A subtype) (Glasgow-1 strain) genomic RNA, prepared according to the technique of Example 3, and with the following oligonucleotides:

PB283 (33 mer) (SEQ ID No. 6)

5′TTGTCGACATGTCTGGAGCCTCTAGTGGGACAG3′

PB284 (42 mer) (SEQ ID No. 7)

5′TTGGATCCTTATTTAATTACTGCAGTTCCAAGGAACTCTC3′

so as to amplify a 3049 bp fragment containing the sequence encoding the Gag protein and the 5′ part of the sequence encoding the Pol protein from the FeLV-A virus (Glasgow-1 strain) (FIG. No. 5 and SEQ ID No. 8) in the form of a SalI-BamHI fragment. After purification, the RT-PCR product was digested with SalI and BamHI to give a 3039 bp SalI-BamHI fragment.

This fragment was ligated with the vector pVR1012 (Example 6), previously digested with SalI and BamHI, to give the plasmid pPB181 (7908 bp) (Figure No. 6 ).

Example 10

Construction of the Plasmid pAB009 (FPV VP2 gene)

A PCR reaction was carried out with the feline panleukopaenia virus (193 strain) genomic DNA (J. Martyn et al., J. Gen. Virol. 1990, 71. 2747-2753), prepared according to the technique of Example 2, and with the following oligonucleotides: AB021 (34 mer) (SEQ ID No. 9)

5′TGCTCTAGAGCAATGAGTGATGGAAGCAGTTCAAC3′

AB024 (33 mer) (SEQ ID No. 10)

5′CGCGGATCCATTAATATAATTTTCTAGGTGCTA3′

so as to amplify a 1776 bp fragment containing the gene encoding the FPV VP2 capsid protein. After purification, the PCR product was digested with XbaI and BamHI in order to give a 1764 bp XbaI-BamHI fragment.

This fragment was ligated with the vector pVR1012 (Example 6), previously digested with XbaI and BamHI, to give the plasmid pAB009 (6664 bp) (FIG. No. 7 ).

Example 11

Construction of the Plasmid pAB053 (FIPV S* gene)

An RT-PCR reaction according to the technique of Example 5 was carried out with the feline infectious peritonitis (FIP) virus (79-1146 strain) genomic RNA (R. de Groot et al., J. Gen. Virol. 1987. 68. 2639-2646), prepared according to the technique of Example 3, and with the following oligonucleotides:

AB103 (38 mer) (SEQ ID No. 11)

5′ATAAGAATGCGGCCGCATGATTGTGCTCGTAACTTGCC3′

AB112 (25 mer) (SEQ ID No. 12)

5′CGTACATGTGGAATTCCACTGGTTG3′

so as to amplify the sequence of the 5′ part of the gene encoding the virus S glycoprotein in the form of an NotI-EcoRI fragment. After purification, the 492 bp RT-PCR product was digested with NotI and EcoRI in order to liberate a 467 bp NotI-EcoRI fragment (fragment A).

The plasmid pJCA089 (Patent Application PCT/FR95/01128) was digested with EcoRI and SpeI in order to liberate a 3378 bp fragment containing the central part of the gene encoding the FIP virus modified S glycoprotein (fragment B).

An RT-PCR reaction according to the technique of Example 5 was carried out with the FIP virus (79-1146 strain) genomic RNA, prepared according to the technique of Example 3, and with the following oligonucleotides:

AB113 (25 mer) (SEQ ID No. 13)

5′AGAGTTGCAACTAGTTCTGATTTTG3′

AB104 (37 mer) (SEQ ID No. 14)

5′ATAAGAATGCGGCCGCTTAGTGGACATGCACTTTTTC3′

so as to amplify the sequence of the 3′ part of the gene encoding the FIP virus S glycoprotein in the form of an SpeI-NotI fragment. After purification, the 543 bp RT-PCR product was digested with SpeI and NotI in order to liberate a 519 bp SpeI-NotI fragment (fragment C).

The fragments A, B and C were then ligated together into the vector pVR1012 (Example 6), previously digested with NotI, to give the plasmid pAB053 (9282 bp), which contains the modified S gene in the correct orientation relative to the promoter (FIG. No. 8 ).

Example 12

Construction of the Plasmid pAB052 (FIPV M gene)

An RT-PC′R reaction according to the technique of Example 5 was carried out with the feline infectious peritonitis (FIP) virus (79-1146 strain) genomic RNA (H. Vennema et al., Virology. 1991, 181. 327-335), prepared according to the technique of Example 3, and with the following oligonucleotides:

AB101 (37 mer) (SEQ ID No. 15)

5′ACGCGTCGACCCACCATGAAGTACATTTTGCTAATAC3′

AB102 (36 mer) (SEQ ID No. 16)

5′CGCGGATCCTTACACCATATGTAATAATTTTTCATG3′

so as to precisely isolate the gene encoding the FIP virus M glycoprotein in the form of a SalI-BamHI fragment. After purification, the 812 bp RT-PCR product was digested with SalI and BamHI in order to liberate a 799 bp SalI-BamHI fragment. This fragment was then ligated into the vector pVR1012 (Example 6), previously digested with SalI and BamHI, to give the plasmid pAB052 (5668 bp) (FIG. No. 9 ).

Example 13

Construction of the Plasmid pAB056 (FIPV N gene)

An RT-PCP reaction according to the technique of Example 5 was carried out with the feline infectious peritonitis (FIP) virus (79-1146 strain) genomic RNA (H. Vennema et ail., Virology. 1991, 181. 327-335), prepared according to the technique of Example 3, and with the following oligonucleotides:

AB106 (35 mer) SEQ ID No. 17)

5′ACGCGTCGACGCCCTGGCCACACAGGGACAACGCG3′

AB107 (36 mer) (SEQ ID No. 18)

5′CGCGGATCCTTAGTCGTAACCTCATCAATCATCTC3′

so as to precisely isolate the gene encoding the FIP virus N protein in the form of a SalI-BamHI fragment. After purification, the 1156 bp RT-PCR product was digested with SalI and BamHI in order to liberate a 1143 bp SalI-BamHI fragment. This fragment was then ligated into the vector pVR1012 (Example 6), previously digested with SalI and BamHI, to give the plasmid pAB056 (6011 bp) (FIG. No. 10 ).

Example 14

Construction of the Plasma pAB028 (FEV gB gene)

A PCR reaction was carried out with the feline herpesvirus (FHV-1) (C27 strain) genomic DNA (S. Spatz et al. Virology. 1993. 197. 125-36) prepared according to the technique of Example 2, and with the following oligonucleotides:

AB061 (36 mer) (SEQ ID No. 19)

5′AAAACTGCAGAATCATGTCCACTCGTGGCGATCTTG3′

AB064 (40 mer) (SEQ ID No. 20)

5′ATAAGAATGCGGCCCCTTAGACAAGATTTGTTTCAGTATC3′

so as to amplify a 2856 bp fragment containing the gene encoding the FHV-1 virus gB glycoprotein in the form of a PstI-NotI fragment. After purification, the PCR product was digested with PstI and NotI to give a 2823 bp PstI-NotI fragment.

This fragment was ligated with the vector pVR1012 (Example 6), previously digested with PstI and NotI, to give the plasmid pAB028 (7720 bp) (FIG. No. 11 ).

Example 15

Construction of the Plasmid pAB029 (FHV gD gene)

A PCR reaction was carried out with the feline herpesvirus (FHV-1) (C-27 strain) genomic DNA (S. Spatz et al. J. Gen. Virol. 1994. 75. 1235-1244), prepared according to the technique of Example 2 and with the following oligonucleotides:

AB065 (36 mer) (SEQ ID No. 21)

5′AAAACTGCAGCCATGATGACACGTCTACATTTTTG3′

AB066 (33 mer) (SEQ ID No. 22)

5′GGAAGATCTTTAAGGATGGTGAGTTGTATGTAT3′

so as to amplify the gene encoding the FHV-1 virus gD glycoprotein in the form of a PstI-BglII fragment. After purification, the 1147 bp PCR product was digested with PstI and BglII in order to isolate a 1129 bp PstI-BglII fragment. This fragment was ligated with the vector pVR1012 (Example 6), previously digested with PstI and BglII, to give the plasmid pAB029 (5982 bp) (FIG. No. 12 ).

Example 16

Construction of the Plasmid pAB010 (FCV C gene)

An RT-PCR reaction according to the technique of Example 5 was carried out with the feline calicivirus (FCV) (F9 strain) genomic RNA (M. Carter et al. Virology. 1992. 190. 443-448), prepared according to the technique of Example 3, and with the following oligonucleotides:

AB025 (33 mer) (SEQ ID No. 23)

5′ACGCGTCGACGCATCTGCTCAACCTGCGCTAAC3′

AB026 (31 mer) (SEQ ID No. 24)

5′CGCGGATCCTCATAACTTAGTCATGGGACTC3′

so as to isolate the gene encoding the FCV virus capsid protein in the form of a SalI-BamHI fragment. After purification, the 2042 bp RT-PCR product was digested with SalI and BamHI in order to isolate a 2029 bp SalI-BamHI fragment. This fragment was ligated with the vector PVR1012 (Example 6), previously digested with SalI and BamHI, to give the plasmid pAB010 (6892 bp) (FIG. No. 13 ).

Example 17

Construction of the Plasmid pAB030 (FIV env gene)

An RT-PCR reaction according to the technique of Example 5 was carried out with the feline immunodeficiency virus (FIV) (Petaluma strain) genomic RNA (R. Olmstec. et al. Proc. Natl. Acad. Sci. USA. 1989. 86. 8083-8096), prepared according to the technique of Example 3, and with the following oligonucleotides:

AB067 (36 mer) (SEQ ID No. 25)

5′AAAACTGCAGAAGGAATGGCAGAAGGATTTGCAGCC3′

AB070 (36 mer) (SEQ ID No. 26)

5′CGCGGATCCTCATTCCTCCTCTTTTTCAGACATGCC3′

so as to amplify a 2592 bp fragment containing the gene encoding the Env glycoprotein from the FIV virus (Petaluma strain, in the form of a PstI-BamHI fragment. After purification, the RT-PCR product was digested with PstI and BamHI to give a 2575 bp PstI-BamHI fragment.

This fragment was ligated with the vector pVR1012 (Example 6), previously digested with PstI and BamHI, to give the plasmid pAB030 (7436 bp) (FIG. No. 14 ).

Example 18

Construction of the Plasmid pAB083 (FIV gag/pro gene)

An RT-PCR reaction according to the technique of Example 5 was carried out with the feline immunodeficiency virus (FIV) (Petaluma strain) genomic RNA (R. Olmsted et al. Proc. Natl. Acad. Sci. USA. 1989. 86. 8088-8096), prepared according to the technique of Example 3, and with the following oligonucleotides:

AB154 (32 mer) (SEQ ID No. 27)

5′ACGCGTCGACATGGGGAATGGACAGGGGCGAG3′

AB155 (33 mer) (SEQ ID No. 28)

5′TGAAGATCTTCACTCATCCCCTTCAGGAAGAGC3′

so as to amplify a 4635 bp fragment containing the gene encoding the Gag and Pro proteins from the FIV virus (Petaluma strain) in the form of a SalI-BglII fragment. After purification, the RT-PCR product was digested with SalI and BglII to give a 4622 bp SalI-BglII fragment.

This fragment was ligated with the vector pVR1012 (Example 6), previously digested with SalI and BglII, to give the plasmid pAB083 (7436 bp) (FIG. No. 15 ).

Example 19

Construction of the Plasmid pAB041 (rabies virus G gene)

An RT-PC′R reaction according to the technique of Example 5 was carried out with the rabies virus (ERA strain) genomic RNA (A. Anilionis et al. Nature. 1981. 294. 275-278), prepared according to the technique of Example 3, and with the following oligonucleotides:

AB011 (33 mer) (SEQ ID No. 29)

5′AAAACTGCAGAGATGGTTCCTCAGGCTCTCCTG3′

AB012 (34 mer) (SEQ ID No. 30)

5′CGCGGATCCTCACACTCTGGTCTCACCCCCACTC3′

so as to amplify a 1589 bp fragment containing the gene encoding the rabies virus G glycoprotein. After purification, the RT-PCR product was digested with PstI and BamHI to give a 1578 bp PstI-BamHI fragment. This fragment was ligated with the vector pVR1012 (Example 6), previously digested with PstI and BamHI, to give the plasmid pAB041 (6437 bp) (FIG. No. 16 ).

Example 20

Production and Purification of the Plasmids

For the preparation of the plasmids intended for the vaccination of animals, any technique may be used which makes it possible to obtain a suspension of purified plasmids predominantly in the supercoiled form. These techniques are well known to persons skilled in the art. There may be mentioned in particular the alkaline lysis technique followed by two successive ultracentrifugations on a caesium chloride gradient in the presence of ethidium bromide as described in J. Sambrook et al. ( Molecular Cloning: A Laboratory Manual , 2nd edition, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989). Reference may also be made to Patent Applications PCT WO 95/21250 and PCT WO 96/02658 which describe methods for producing, on an industrial scale, plasmids which can be used for vaccination. For the purposes of the manufacture of vaccines (see Example 17), the purified plasmids are resuspended so as to obtain solutions at a high concentration (>2 mg/ml) which are compatible with storage. To do this the plasmids are resuspended either in ultrapure water or in TE buffer (10 mM Tris-HCl; 1 mM EDTA, pH 8.0).

Example 21

Manufacture of the Associated Vaccines

The various plasmids necessary for the manufacture of an associated vaccine are mixed starting with their concentrated solutions (Example 16). The mixtures are prepared such that the final concentration of each plasmid corresponds to the effective dose of each plasmid. The solutions which can be used to adjust the final concentration of the vaccine may be either a 0.9% NaCl solution, or PBS buffer.

Specific formulations such as liposomes, cationic lipids, may also be used for the manufacture of the vaccines.

Example 22

Vaccination of Cats

The cats are vaccinated with doses of 10 μg, 50 μg or 250 μg per plasmid.

The injections are performed with a needle by the intramuscular route. In this case, the vaccinal doses are administered in a volume of 1 ml.

The injections can also be performed with a needle by the intradermal route. In this case, the vaccinal doses are administered in a total volume of 1 ml administered at 10 points of 0.1 ml or at 20 points of 0.05 ml. The intradermal administrations are performed after shaving the skin (thoracic flank in general) or at the level of a relatively glabrous anatomical region, for example the inner surface of the thigh.

A liquid jet injection apparatus (with no needle) can also be used for the intradermal injections.

30 1 29 DNA Feline leukemia virus 1tttgtcgacc atggaaagtc caacgcacc 29 2 28 DNA Feline leukemia virus 2tttggatcct catggtcggt ccggatcg 28 3 29 DNA Feline leukemia virus 3tttgtcgaca tggaaggtcc aacgcaccc 29 4 32 DNA Feline leukemia virus 4ttggatcctc atggtcggtc cggatcatat tg 32 5 1989 DNA Feline leukemia virus 5atggaaggtc caacgcaccc aaaaccctct aaagataaga ctttctcgtg ggacctaatg 60attctggtgg gggtcttact aagactggac gtgggaatgg ccaatcctag tccgcaccaa 120atatataatg taacttggac aataaccaac cttgtaactg gaacaaaggc taatgccacc 180tccatgttgg gaaccctgac agacgccttc cctaccatgt attttgactt atgtgatata 240ataggaaata catggaaccc ttcagatcaa gaaccattcc cagggtatgg atgtgatcag 300cctatgagga ggtggcgaca gagaaacaca cccttttatg tctgtccagg acatgccaac 360cggaagcaat gtggggggcc acaggatggg ttctgcgctg tatggggttg cgagaccacc 420ggggaaacct attggagacc cacctcctca tgggactaca tcacagtaaa aaaaggggtt 480actcagggaa tatatcaatg tagtggaggt ggttggtgtg ggccctgtta cgataaagct 540gttcactcct cgacaacggg agctagtgaa gggggccggt gcaacccctt gatcttgcaa 600tttacccaaa agggaagaca aacatcttgg gatggaccta agtcatgggg gctacgacta 660taccgttcag gatatgaccc tatagccctg ttctcggtat cccggcaagt aatgaccatt 720acgccgcctc aggccatggg accaaatcta gtcctgcctg atcaaaaacc cccatccagg 780caatctcaaa tagagtcccg agtaacacct caccattccc aaggcaacgg aggcacccca 840ggtgtaactc ttgttaatgc ctccattgcc cctctacgta cccctgtcac ccccgcaagt 900cccaaacgta tagggaccgg aaataggtta ataaatttag tgcaagggac atacctagcc 960ttaaatgcca ccgaccccaa caaaactaaa gactgttggc tctgcctggt ttctcgacca 1020ccttattacg aagggattgc aatcttaggt aactacagca accaaacaaa cccctcccca 1080tcctgcctat ctactccgca acataagcta actatatctg aggtgtcagg gcaaggactg 1140tgcataggga ctgttcctaa gacccaccag gctttgtgca ataagacaca acagggacat 1200acaggggctc actatctagc cgcccccaat ggcacctatt gggcctgtaa cactggactc 1260accccatgca tttccatggc agtgctcaat tggacctctg atttttgtgt cttaatcgaa 1320ttatggccca gagtgaccta ccatcaaccc gaatacattt acacacattt cgacaaagct 1380gtcaggttcc gaagagaacc aatatcacta accgttgccc ttataatggg aggactcact 1440gtagggggca tagccgcggg ggtcggaaca gggactaaag ccctccttga aacagcccag 1500ttcagacaac tacaaatggc tatgcacgca gacatccagg ccctagaaga gtcaattagt 1560gccttagaaa aatccctgac ctccctctcc gaggtagtct tacaaaatag acggggccta 1620gatattctgt tcttacaaaa gggagggctc tgtgccgcct taaaggaaga atgctgcttc 1680tatgcagatc acaccggact cgtcagagac aatatggcta aattaagaga aagactgaaa 1740cagcgacaac aactgtttga ctcccaacag ggatggtttg aaggatggtt caacaagtcc 1800ccctggttta caaccctaat ttcctccatt ataggcccct tactaatcct actcctaatt 1860ctcctcttcg gcccatgcat ccttaaccga ttagtgcaat tcgtaaaaga cagaatatct 1920gtggtacaag ccttaatttt aacccaacag taccaacaga tacagcaata tgatccggac 1980cgaccatga 1989 6 33 DNA Feline leukemia virus 6ttgtcgacat gtctggagcc tctagtggga cag 33 7 40 DNA Feline leukemia virus 7ttggatcctt atttaattac tgcagttcca aggaactctc 40 8 3022 DNA Feline leukemia virus 8atgtctggag cctctagtgg gacagccatt ggggctcatc tgtttggggt ctcacctgaa 60tacagggtgt tgatcggaga cgagggagcc ggaccctcaa ggtctctttc tgaggtttca 120ttttcggttt ggtaccaaag acgcgcggca cgtcttgtca ttttttgtct ggttgcgtct 180tttcttgtcc cttgtctaac ctttttaatt gcagaaaccg tcatgggcca aactataact 240acccccttaa gcctcaccct tgatcactgg tctgaagtcc gggcacgagc ccataatcaa 300ggtgtcgagg tccggaaaaa gaaatggatt accttatgtg aggccgaatg ggtgatgatg 360aatgtgggct ggccccgaga aggaactttt tctcttgata gcatttccca ggttgaaaag 420aagatcttcg ccccgggacc atatggacac cccgaccaag ttccttacat tactacatgg 480agatccttag ccacagaccc cccttcgtgg gttcgtccgt tcctaccccc tcccaaacct 540cccacacccc tccctcaacc tctttcgccg cagccctccg cccctcttac ctcttccctc 600taccccgttc tccccaagcc agaccccccc aaaccgcctg tgttaccgcc tgatccttct 660tcccctttaa ttgatctctt aacagaagag ccacctccct atccgggggg tcacgggcca 720ccgccatcag gtcctaggac cccaaccgct tccccgattg caagccggct aagggaacga 780cgagaaaacc ctgctgaaga atcgcaagcc ctccccttga gggaaggccc caacaaccga 840ccccagtatt ggccattctc agcttcagac ttgtataact ggaagtcgca taacccccct 900ttctcccaag atccagtggc cctaactaac ctaattgagt ccattttagt gacgcatcaa 960ccaacctggg acgactgcca gcagctcttg caggcactcc tgacaggcga agaaaggcaa 1020agggtccttc ttgaggcccg aaagcaggtt ccaggcgagg acggacggcc aacccaacta 1080cccaatgtca ttgacgagac tttccccttg acccgtccca actgggattt tgctacgccg 1140gcaggtaggg agcacctacg cctttatcgc cagttgctat tagcgggtct ccgcggggct 1200gcaagacgcc ccactaattt ggcacaggta aagcaggttg tacaagggaa agaggaaacg 1260ccagcagcat ttttagaaag attaaaagag gcttatagaa tgtacactcc ctatgaccct 1320gaggacccag ggcaagcggc tagtgttatc ctatccttta tataccagtc tagcccagat 1380ataagaaata agttacaaag gctagaaggc ctacaagggt tcaccctatc tgatctgcta 1440aaagaggcag aaaagatata caacaaaagg gagaccccag aggaaaggga agaaagatta 1500tggcagcgac aggaagaaag agataaaaag cgccacaagg agatgactaa agttctggcc 1560acagtagttg ctcagaatag agataaggat agagaagaaa gtaaactggg ggatcaaagg 1620aaaatacctc tggggaaaga ccagtgtgcc tattgcaagg aaaaggggca ttgggttcgc 1680gattgcccca aacgacccag gaagaaaccc gccaactcca ctctcctcaa cttaggagat 1740aggagagtca gggccaggac ccccccccct gagcccagga taaccttaaa aatagggggg 1800caaccggtga cttttctggt ggacacggga gcccagcact cagtactgac tcgaccagat 1860ggacctctca gtgaccgcac agccctggtg caaggagcca cgggaagcaa aaactaccgg 1920tggaccaccg acaggagggt acaactggca accggtaagg tgactcattc ttttttatat 1980gtacctgaat gtccctaccc gttattaggg agagacctat taactaaact taaggcccaa 2040atccatttta ccggagaagg ggctaatgtt gttgggccca ggggtttacc cctacaagtc 2100cttactttac aattagaaga ggagtatcgg ctatttgagc cagaaagtac acaaaaacag 2160gagatggaca cttggcttaa aaactttccc caggcgtggg cagaaacagg aggtatggga 2220atggctcatt gtcaagcccc cgttctcatt caacttaagg ctactgccac tccaatctcc 2280atccgacagt atcctatgcc ccatgaagcg taccagggaa ttaagcctca tataagaaga 2340atgctagatc aaggcatcct caagccctgc cagtccccat ggaatacacc cttattacct 2400gttaagaagc cagggaccga ggattacaga ccagtgcagg acttaagaga agtaaacaaa 2460agagtagaag acatccatcc tactgtgcca aatccatata acctccttag caccctcccg 2520ccgtctcacc cttggtacac tgtcctagat ttaaaggacg cttttttctg cctgcgacta 2580cactctgaga gtcagttact ttttgcattt gaatggagag atccagaaat aggactgtca 2640gggcaactaa cctggacacg ccttcctcag gggttcaaga atagccccac cctatttgat 2700gaggccctgc actcagacct ggccgatttc agggtaaggt acccggctct agtcctccta 2760caatatgtag atgacctctt gctggctgcg gcaaccagga ctgaatgcct ggaagggact 2820aaggcactcc ttgagacttt gggcaataag gggtaccgag cctctggaaa gaaggcccaa 2880atttgcctgc aagaagtcac atacctgggg tactctttaa aagatggcca aaggtggctt 2940accaaagctc ggaaagaagc catcctatcc atccctgtgc ctaaaaaccc acgacaagtg 3000agagagttcc ttggaactgc ag 3022 9 34 DNA Feline Panleukopaenia Virus 9tgctctagag caatgagtga tggagcagtt caac 34 10 33 DNA Feline Panleukopaenia Virus 10cgcggatcca ttaatataat tttctaggtg cta 33 11 38 DNA Feline infectious peritonitis virus 11ataagaatgc ggccgcatga ttgtgctcgt aacttgcc 38 12 25 DNA Feline infectious peritonitis virus 12cgtacatgtg gaattccact ggttg 25 13 25 DNA Feline infectious peritonitis virus 13agagttgcaa ctagttctga ttttg 25 14 37 DNA Feline infectious peritonitis virus 14ataagaatgc ggccgcttag tggacatgca ctttttc 37 15 37 DNA Feline infectious peritonitis virus 15acgcgtcgac ccaccatgaa gtacattttg ctaatac 37 16 36 DNA Feline infectious peritonitis virus 16cgcggatcct tacaccatat gtaataattt ttcatg 36 17 35 DNA Feline infectious peritonitis virus 17acgcgtcgac gccatggcca cacagggaca acgcg 35 18 36 DNA Feline infectious peritonitis virus 18cgcggatcct tagttcgtaa cctcatcaat catctc 36 19 36 DNA Feline herpesvirus 1 19aaaactgcag aatcatgtcc actcgtggcg atcttg 36 20 40 DNA Feline herpesvirus 1 20ataagaatgc ggccgcttag acaagatttg tttcagtatc 40 21 36 DNA Feline herpesvirus 1 21aaaactgcag ccaatgatga cacgtctaca tttttg 36 22 33 DNA Feline herpesvirus 1 22ggaagatctt taaggatggt gagttgtatg tat 33 23 33 DNA Feline calicivirus 23acgcgtcgac gcatgtgctc aacctgcgct aac 33 24 31 DNA Feline calicivirus 24cgcggatcct cataacttag tcatgggact c 31 25 36 DNA Feline immunodeficiency virus 25aaaactgcag aaggaatggc agaaggattt gcagcc 36 26 36 DNA Feline immunodeficiency virus 26cgcggatcct cattcctcct ctttttcaga catgcc 36 27 32 DNA Feline immunodeficiency virus 27acgcgtcgac atggggaatg gacaggggcg ag 32 28 33 DNA Feline immunodeficiency virus 28tgaagatctt cactcatccc cttcaggaag agc 33 29 33 DNA Rabies virus 29aaaactgcag agatggttcc tcaggctctc ctg 33 30 34 DNA Rabies virus 30cgcggatcct cacagtctgg tctcaccccc actc 34

Claims

1. An immunogenic composition for inducing in a feline host an immunological response against infectious peritonitis virus comprising a plasmid that contains and expresses in vivo in a feline host cell a nucleic acid molecule having a sequence encoding infectious peritonitis virus M protein.

2. The immunogenic composition of claim 1 wherein the plasmid further comprises a cytomeglovirus early (CMV-IE) promoter operatively linked to the nucleic acid molecule.

3. The immunogenic composition of claim 1 further comprising a live whole vaccine against a feline pathogen, or an inactivated whole vaccine against a feline pathogen, or recombinant vaccine against a feline pathogen, or a subunit vaccine against a feline pathogen.

4. A method for inducing an immunological response in a feline comprising: administering to said feline an immunogenic composition as claimed in any one of claims 1, 2, 3.

5. A method for inducing an immunological response in a feline comprising: administering to said feline an immunogenic composition selected from the group consisting of a live whole vaccine, an inactivated whole vaccine, a subunit vaccine, and a recombinant vaccine; and thereafter, administering to said feline an immunogenic composition as claimed in any one of claims 1, 2, or 3.

6. A method for inducing an immunological response in a feline comprising: administering to said feline an immunogenic composition selected from the group consisting of a live whole vaccine, an inactivated whole vaccine, a subunit vaccine, and a recombinant vaccine; and two to six weeks thereafter, admire stering to, said feline an immunogenic composition as claimed in any one of claims 1, 2, or 3.

7. A method for inducing an immunological response in a feline comprising: administering to said feline an immunogenic composition as claimed in any one of claims 1, 2, or 3 in combination with a live whole vaccine against a feline pathogen, or an inactivated whole vaccine against a feline pathogen, or recombinant vaccine against a feline pathogen, or a subunit vaccine against a feline pathogen.

8. A kit comprising (i) an immunogenic composition according to claim 1, and (ii) a feline vaccine selected from the group consisting of a live whole vaccine, an inactivated whole vaccine, a subunit vaccine, and recombinant vaccine.

9. A plasmid that contains and expresses in vivo in a feline host cell a nucleic acid molecule having a sequence encoding infectious peritonitis virus M protein. infectious peritonitis virus M protein.

10. A vaccine for inducing in a feline host an immunological response against infectious peritonitis virus comprising a plasmid that contains and expresses in vivo in a feline host cell a nucleic acid molecule having a sequence encoding infectious peritonitis virus M protein.

11. The vaccine of claim 10 wherein the plasmid further comprises a cytomeglovirus early (CMV-IE) promoter operatively linked to the nucleic acid molecule.

12. The vaccine of claim 10 further comprising a live whole vaccine against a feline pathogen, or an inactivated whole vaccine against a feline pathogen, or recombinant vaccine against a feline pathogen, or a subunit vaccine against a feline pathogen.

13. A method for vaccinating a feline comprising: administering to said feline a vaccine as claimed in any one of claims 10, 11, or 12.

14. A method for vaccinating a feline comprising: administering to said feline a vaccine selected from the group consisting of a live whole vaccine, an inactivated whole vaccine, a subunit vaccine, and a recombinant vaccine; and thereafter, administering to said feline a vaccine as claimed in any one of claims 10, 11, or 12.

15. A method vaccinating a feline comprising: administering to said feline a vaccine selected from the group consisting of a live whole vaccine, an inactivated whole vaccine, a subunit vaccine, and a recombinant vaccine; and two to six weeks thereafter, administering to said feline a vaccine composition as claimed in any one of claims 10, 11 or 12.

16. A method for vaccinating a feline comprising: administering to said feline a vaccine as claimed in any one of claims 10, 11, or 14 in combination with a live whole vaccine against a feline pathogen, or an inactivated whole vaccine against a feline pathogen, or recombinant vaccine against a feline pathogen, or a subunit vaccine against a feline pathogen.