Patent Application
20180223371
The present invention relates to selection of subjects having tumors for treatment with an FGFR inhibitor as well as to the treatment of such subjects with such inhibitor.
Fibroblast growth factors and their receptors (FGFR) drive important developmental signaling pathways that affect cell proliferation, migration and survival. Aberrant FGF signaling plays a role in many cancers. Turner, N. and Grose, R. (2010) Nat. Rev. Cancer 10: 116-29. The FGFR family consists of FGFR1, FGFR2, FGFR3 and FGFR4. FGFR are tyrosine kinases that are activated in a fraction of tumors by gene amplification, mutations, or chromosomal translocations or rearrangements. Amplification of FGFR1 occurs in squamous cell lung carcinoma and estrogen receptor-positive breast cancer. FGFR2 is also amplified in gastric and breast cancers. Mutations in FGFR2 are observed in endometrial cancer and of FGFR3 in bladder cancer. The Cancer Genome Atlas Network (2012) Nature 489: 519-25; Elbauomy Elsheikh, S. et al. (2007) Breast Cancer Res. 9: R23; Turner, N. et al. (2010) Cancer Res. 70: 2085-94; Peng, D. F. et al. (2003) J. Pathol. 201: 439-50; Matsumoto, K. et al. (2012) Br. J. Cancer 106: 727-32; Byron, S. A. et al. (2008) Cancer Res. 68: 6902-7; Cappellen, D. et al. (1999) Nat. Genet. 23: 18-20; Al-Ahmadie, H. A. et al. (2011) J. Pathol. 224: 270-9. Chromosomal translocations and rearrangements of FGFR1, FGFR2 and FGFR3 were reported in various cancers. Parker, B. C. et al. (2014) J. Pathol. 232: 4-15.
FGFR fusion genes were also reported in various cancers, both in hematological and solid tumor types. For example, FGFR1-ERLIN2 in breast cancer, FGFR2-KIAA1967 in squamous cell lung cancer, FGFR3 translocation t(4,14) in multiple myeloma. It is noted that some fusions occur in different cancers. FGFR3-TACC3 fusions occur, for example, in glioblastoma, bladder cancer and squamous cell carcinoma. Comprehensive listings of known fusion genes are found, e.g., in Annala et al. (2013) or in Shaw, A. T. et al. (2013) Nature Reviews Cancer 13: 772-87.
Responding to a clear need, a number of more or less specific FGFR inhibitors were developed. They include PD173074 (Mohammadi et al. (1998) EMBO J. 17: 5896-904), Pazopanib (Harris et al. (2009) J. Med. Chem. 51: 4632-40; Keisner and Shah (2011) Drugs 71: 443-54), AZD4547 (Gavine et al. (2012) Cancer Res. 72: 2045-56), Ponatinib (or AP24534) (Huang et al. (2010) J. Med. Chem. 53: 4701-19), Dovitinib (Trudel et al.
(2005) Blood 105: 2941-8; Man et al. (2014) J. Cell, Mol. Med. 18: 143-55), BGJ398 (Guagnano et al. (2011) J. Med. Chem. 54: 7066-83), E-3810 also known as Lucitanib (Bello et al. (2011) Cancer Res. 71: 1396-405), JNJ-42756493 (Squires et al. (2008) AACR Abstract 1545), ARQ 087 (Yu et al. (2011) Cancer Res. 71 (Suppl. 1) 3671), LY2874455 (Zhao et al. (2011) Mol Cancer Ther. 10: 2200-10), BAY1163877 (Heroult et al. (2014) Cancer Res. 74 (Suppl. 19)—Abstract 1739), ASP5878 (73rd Annual Meeting of the Japanese Cancer Association (2014)—Abstract/Poster 1411), E7090 (Saori Watanabe Miyano et al. (2015) AACR Abstract 770), ODM-203 (Holmström et al. 26th ENA Symposium (2014) Eur. J. Cancer 50(56):142 - Abstract 432), Nintedanib (Roth et al. (2015) J Med Chem. 58: 1053-63), TAS-120 (Ochiiwa, et al. (2013) AACR; Mol. Cancer Ther. 12 (11 Suppl), Abstract A270), PRN 1109 and PRN 1371 (both in: Phan V T. et al. 26th ENA Symposium (2014) Eur. J. Cancer 50(56):157 - Abstract 483). They also include the inhibitory aminopyrazole derivatives and their pharmaceutically acceptable salts described in international patent publication WO 2011/016528, including in particular 5-amino-1-(2-methyl-1H-benzimidazol-5-yl)-1H-pyrazol-4-yl]-(1H-indol-2-yl)-methanone, herein referred to as Compound A.
Now that several FGFR inhibitors are being administered to human subjects, the question arises as to the proper criteria for determining whether a particular subject should or should not be treated with a particular inhibitor. The question relates to whether an inhibitor is active against a tumor harboring a mutationally activated FGFR, a tumor expressing an
FGFR fusion protein and/or a tumor containing an amplified, unmutated or mutated FGFR gene. A related question concerns the specificity for particular FGFR family members. Answers to these questions may be inhibitor-specific. Present practice appears to be to treat any tumor containing any of the above-described genetic alterations in an FGFR gene with the preferred FGFR inhibitor. Clearly, proper selection criteria are not yet known.
The present invention relates to a method for identifying and selecting a group of subjects diagnosed with a cancer that are likely to respond to a therapeutic regimen that comprises administration of a pharmaceutical composition comprising an effective amount of Compound A. The method comprises taking a tumor or liquid biopsy from a subject suffering from a cancer, determining the levels of expression of FGFR1, FGFR2 and FGFR3, and if the determined level of expression of at least one of FGFR1, FGFR2 and FGFR3 exceeds a pre-established threshold level for that FGFR, considering or declaring the subject eligible for the therapeutic regimen.
In particular embodiments, the above-described method determines levels of expression of FGFR1, FGFR2 and FGFR3 at the messenger RNA (mRNA) level, Messenger RNA levels may be assessed by any suitable method. Preferred is detection of FGFR mRNA molecules by digital methods. A specific method of this type is the nCounter Gene Expression Assay of NanoString used herein. Another suitable method is reverse transcription and quantitative PCR.
To establish threshold levels for each of FGFR1, FGFR2 and FGFR3, a balanced set of patient-derived tumor xenograft (PDX) models is assembled which models fit into four different categories which are similarly represented in the set:
(i) increased copy number of an FGFR gene and overexpression of an FGFR, (ii) increased copy number of an FGFR gene without overexpression of an FGFR, (iii) no increase in copy number of an FGFR gene but overexpression of an FGFR, and (iv) expression of an FGFR fusion gene product.
Levels of expression of FGFR1, FGFR2 and FGFR3 and efficacy of treatment with Compound A are measured. These data establish the relationship between response rates and percentile cutoff levels of FGFR expression. See Table 1. “Response Rate” (sometimes abbreviated as “RR”) refers to the percentage of models whose tumors are effectively treated by Compound A, i.e., that meet the required minimal treatment efficacy ΔT/ΔC (here 0). A percentile cutoff level of expression of an FGFR refers to a level at which a percentile of models has the same or a lower expression level for the FGFR. For example, the 75th percentile cutoff level of expression with respect to an FGFR identifies a level of the FGFR (threshold level) that corresponds to the highest level measured in the 75% of models having the lowest levels of the FGFR. It is noted that, whereas Table 1 provides threshold levels for certain percentile cutoff levels of expression, threshold levels can be easily determined for other percentile cutoff levels. The data needed for such determinations are provided in Table 2 below.
Threshold FGFR levels corresponding to cutoff levels of between about 44% and about 73% may be selected as this range of threshold levels does not exclude any model that is capable of responding to Compound A while substantially increasing the response rate observed within the models considered overexpressed, as compared to the response rate observed within all models (e.g. by more than 10%). Corresponding response rates are from about 36.2% to about 52.5%. More preferred are threshold FGFR levels corresponding to cutoff levels from about 60% to about 73%. Corresponding response rates are from about 39.6% to about 52.5%. Even more preferred are threshold FGFR levels corresponding to cutoff levels from about 65% to about 73%. Corresponding response rates are from about 42.9% to about 52.5%. Most preferred are threshold FGFR levels corresponding to cutoff levels from about 70% to about 73%. Less preferred threshold values—because of lower response rates - are those corresponding to cutoff levels below about 44%. Also less preferred because a fraction of treatable subjects is excluded are those corresponding to cutoff levels exceeding 73%.
It is noted that for the purposes of the indication-independent diagnostic method discussed here, FGFR1, 2 and 3 were considered in the calculations for each model, i.e. a model was considered overexpressed if the expression level of at least one of FGFR1, FGFR2 or FGFR3 exceeds the corresponding threshold level for the chosen percentile cutoff level.
In other particular embodiments of the above-described method, levels of expression of FGFR1, FGFR2 and FGFR3 are assessed at the protein level. Suitable methods include Western blotting, dot blotting, ELISA, immunochromatography, immunohistochemistry, mass spectrometry and flow cytometry.
The above-described method does not discriminate between cancer indications, i.e. is indication-independent. The invention also encompasses methods for identifying and selecting a group of subjects diagnosed with a specific type of cancer, e.g., esophageal cancer, lung cancer (e.g. squamous NSCLC) or bladder cancer, that are likely to respond to a therapeutic regimen that comprises administration of a pharmaceutical composition comprising an effective amount of Compound A. The method comprises taking a tumor or liquid biopsy from a subject suffering from the latter type of cancer, determining the level of expression of the FGFR (or multiple FGFR) known to be elevated in tumors of a fraction of subjects suffering from cancers of this type, and if the determined level of expression of the FGFR exceeds a pre-established threshold level, considering or declaring the subject eligible for the therapeutic regimen.
To establish a threshold level for the FGFR in question (or multiple FGFR), a balanced set of tumor xenograft (PDX) models for the specific type of cancer concerned may be assembled, whereby the models typically fit into four different categories which are similarly represented in the set:
(i) increased copy number of the FGFR gene and overexpression of the FGFR, (ii) increased copy number of the FGFR gene without overexpression of the FGFR, (iii) no increase in copy number of the FGFR gene but overexpression of an FGFR, and (iv) expression of an FGFR fusion gene product.
Levels of expression of the FGFR in question as well as efficacy of treatment with Compound A are measured. These data establish the relationship between response rates and percentile cutoff levels of FGFR expression. Threshold FGFR levels corresponding to cutoff levels or ranges of cutoff levels may be selected that are as elevated as possible without excluding any model that is capable of responding to Compound A (see the above discussion of the indication-independent method). Based on the selection objective, higher or lower percentile cutoff levels may be chosen.
The present invention also relates to a method of personalized cancer therapy comprising the above-described method of taking a tumor or liquid biopsy from a subject suffering from a cancer, determining the levels of expression of any or all of FGFR1, FGFR2 and
FGFR3, and, if the determined level of expression of at least one of FGFR1, FGFR2 and FGFR3 exceeds a pre-established threshold level, considering or declaring the subject eligible for the therapeutic regimen, and adding the further step of subjecting the subject to a therapeutic regimen that comprises administration of a pharmaceutical composition comprising an effective amount of Compound A. In a more specific embodiment, the latter method of personalized cancer therapy may be stratified by cancer indication.
The present invention also relates to a method of selecting a subject suffering from a cancer for treatment with Compound A, the method comprising determining the levels of expression of FGFR1, FGFR2 and FGFR3 in a tumor or liquid biopsy from the subject and if the determined level of expression of at least one of FGFR1, FGFR2 and FGFR3 exceeds a pre-established threshold level, considering the subject eligible for treatment.
The present invention further relates to the use of Compound A in the preparation of a medicament for treating cancer in a subject in need thereof, or to Compound A for use in the treatment of cancer in a subject, wherein the level of expression of at least one of FGFR1, FGFR2 and FGFR3 as determined in a tumor or liquid biopsy from the subject exceeds a pre-established threshold level.
In a specific embodiment of the above-described methods and uses, the levels of expression of FGFR1, FGFR2 and FGFR3 are measured at the messenger RNA level and the pre-established threshold level of expression of at least one FGFR corresponds to any level from the 44% to the 73% cutoff level of expression for the at least one FGFR, corresponding to expression levels relative to the median of levels of mRNA expression of a set of 16 reference genes measured by the nCounter Gene Expression Assay from 0.104 to 0.641 for FGFR1, from 0.257 to 1.094 for FGFR2, and from 0.128 to 0.815 for FGFR3, the 16 reference genes being ACTB, ALAS1, CLTC, MRPL19, RPL19, RPLP0, SF3A1, TBP, TUBB, c1orf43 exon 1, c1 or143 exon 2, CHMP2A exon 3, EMC7 exon 5, EMC7 exon 3, GPI exon 4 and GPI exon 6.
In yet a more specific embodiment, the pre-established threshold level of at least one FGFR corresponds to any level from the 60% to the 73% cutoff level of expression for the at least one FGFR, corresponding to expression levels relative to the median of levels of mRNA expression of the above-defined set of 16 reference genes measured by the nCounter Gene Expression Assay from 0.301 to 0.641 for FGFR1, from 0.669 to 1.094 for FGFR2, and from 0.289 to 0.815 for FGFR3. In yet a more specific embodiment, the pre-established threshold level of at least one FGFR corresponds to any level from the 65% to the 73% cutoff level of expression for the at least one FGFR, corresponding to expression levels relative to the median of levels of mRNA expression of the above-defined set of 16 reference genes measured by the nCounter Gene Expression Assay from 0.484 to 0.641 for FGFR1, from 0.884 to 1.094 for FGFR2, and from 0.490 to 0.815 for FGFR3. In yet a more specific embodiment, the pre-established threshold level of at least one FGFR corresponds to any level from the 70% to the 73% cutoff level of expression for the at least one FGFR, corresponding to expression levels relative to the median of levels of mRNA expression of a set of the above-defined 16 reference genes measured by the nCounter Gene Expression Assay from 0.558 to 0.641 for FGFR1, from 0.984 to 1.094 for FGFR2, and from 0.671 to 0.815 for FGFR3.
In another specific embodiment of the present invention, the cancer is gastric cancer and the pre-established threshold level of expression of FGFR2 corresponds to any level from the 44% to the 83% cutoff level of expression for FGFR2, corresponding to expression levels relative to the median of levels of mRNA expression of the above-defined set of 16 reference genes measured by the nCounter Gene Expression Assay from 0.257 to 1.610 for FGFR2. In such embodiment, more preferred are threshold FGFR2 levels corresponding to cutoff levels from about 60% and about 83% (0.669-1.610). Even more preferred are threshold FGFR2 levels corresponding to cutoff levels from about 70% and about 83% (0.984-1.610). Most preferred are threshold FGFR2 levels corresponding to cutoff levels from about 80% and about 83% (1.460-1.610). In such embodiment, the level of expression of FGFR2 only, or of FGFR1, 2 and 3, may be determined.
In yet another embodiment of the present invention, the cancer is esophageal squamous-cell carcinoma and the pre-established threshold level of expression of FGFR1 corresponds to any level from the 44% to the 84% cutoff level of expression for FGFR1, corresponding to expression levels relative to the median of levels of mRNA expression of the above-defined set of 16 reference genes measured by the nCounter Gene Expression Assay from 0.104 to 1.362 for FGFR1. In such embodiment, more preferred are threshold FGFR1 levels corresponding to cutoff levels from about 60% and about 84% (0.301-1.362). Even more preferred are threshold FGFR1 levels corresponding to cutoff levels from about 70% and about 84% (0.558-1.362). Most preferred are threshold FGFR1 levels corresponding to cutoff levels from about 80% and about 84% (0.759-1.362). In such embodiment, the level of expression of FGFR1 only, or of FGFR1, 2 and 3, may be determined.
All the above-mentioned threshold levels, as well as threshold levels for some intermediate percentile cutoff levels, are found in Table 1. As mentioned previously, threshold levels can be easily determined for other percentile cutoff levels.
Definitions:
Compound A is 5-amino-1-(2-methyl-1H-benzimidazol-5-yl)-1H-pyrazol-4-yl]-(1H-indol-2-yl)-methanone (CAS 1265229-25-1). The compound was described in international patent application publication WO 2011016528 and Nakanishi, Y. et al. (2014) Mol. Cancer Ther. 13: 2547-58. It also includes pharmaceutically acceptable salts of such compound, including but not limited to acetic acid, adipic acid, L-ascorbic acid, L-aspartic acid, capric acid (decanoic acid), carbonic acid, citric acid, fumaric acid, galactaric acid, D-glucoheptonic acid, D-gluconic acid, D-glucuronic acid, glutamic acid, glutaric acid, glycerophosphoric acid, glycolic acid, hippuric acid, hydrochloric acid, DL-lactic acid, lauric acid, maleic acid, (−)-L-malic acid, palmitic acid, phosphoric acid, sebacic acid, stearic acid, succinic acid, sulfuric acid, (+)-L-tartaric acid and thiocyanic acid, where (−)-L-malic acid is preferred.
“FGFR” refers to a member of the family of fibroblast growth factor receptors. The FGFR family is a member of the receptor tyrosine kinase family. Four members of the FGFR family are known, i.e., FGFR1, FGFR2, FGFR3 and FGFR4. The FGFR as referred to in the present invention may be from any origin, but preferably from a mammalian and, more preferably, from a human origin. The present invention is concerned with FGFR1, 2 and/or 3, and the term FGFR as used herein refers to any or any combination of the latter three FGFR species.
“Cancer” generally refers to malignant neoplasm, which may be metastatic or non-metastatic. For instance, non-limiting examples of cancer that develops from epithelial tissues such as gastrointestinal tract and skin include brain tumor, skin cancer, head and neck cancer, esophageal cancer, lung cancer, stomach cancer, duodenal cancer, breast cancer, prostate cancer, cervical cancer, cancer of uterine body, pancreatic cancer, liver cancer, cholangiocarcinoma, gallbladder cancer, colorectal cancer, colon cancer, bladder cancer, and ovarian cancer. Non-limiting examples of sarcoma that develops from non-epithelial tissues (stroma) such as muscles include osteosarcoma, chondrosarcoma, rhabdomyosarcoma, leiomyosarcoma, liposarcoma, and angiosarcoma. Furthermore, non-limiting examples of hematological cancer derived from hematopoietic organs include malignant lymphoma including Hodgkin's lymphoma and non-Hodgkin's lymphoma, leukemia including acute myelocytic leukemia, chronic myelocytic leukemia, acute lymphatic leukemia, chronic lymphatic leukemia, and multiple myeloma. The latter examples of cancer are also referred to herein as types of cancer.
By a “therapeutically effective amount” of Compound A (also referred to herein as the “active agent” or the “compound”) is meant an amount of the compound which, subsequent to single or multiple administration, confers a therapeutic effect on the treated subject, at a reasonable benefit/risk ratio applicable to any medical treatment. However, it is understood that effective doses will also vary depending on route of administration, as well as the possibility of co-usage with other agents, including anti-neoplastic agents. It will be understood, however, that the total daily usage of the compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular patient will depend upon a variety of factors including the severity of the condition/disease; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, the route of administration, the rate of excretion of the active agent, the duration of the treatment; drugs used in combination or contemporaneously with the active agent, and like factors well known in the medical arts.
The term “pharmaceutically acceptable carrier” or “pharmaceutically acceptable vehicle” encompasses any of the standard pharmaceutical carriers, solvents, surfactants, or vehicles. Suitable pharmaceutically acceptable vehicles include aqueous vehicles and non-aqueous vehicles. Standard pharmaceutical carriers and their formulations are described, in a non-limiting fashion, in Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa., 19th ed. 1995.
The term “subject” relates to a mammalian animal and, preferably, to a human person. The term “overexpression” relating to an FGFR means a level of expression (measured at the RNA or the protein level) in a tumor or a tumor biopsy that exceeds the level measured in the corresponding non-tumorous cells or tissue.
Methodology:
Sensitivity to treatment with FGFR kinase inhibitor Compound A was examined in 66 patient-derived xenograft (PDX) models. PDX models were selected based on available information that had been obtained by RNA sequencing (RNA-Seq) using next-generation sequencing (NGS) technology and hybridization to an Affymetrix Human Genome U219 array plate. A balanced set of models was selected which had the following characteristics: (i) an increased copy number of an FGFR gene and overexpression of an FGFR, or (ii) an increased copy number of an FGFR gene without overexpression of an FGFR, or (iii) no increase in copy number of an FGFR gene but overexpression of an FGFR, or (iv) expression of an FGFR fusion gene product.
For each tumor model, the effects of Compound A on tumor growth (treatment efficacy), FGFR copy number and FGFR mRNA levels were assessed (or re-assessed). In several model studies, FGFR protein levels were also estimated using immunohistochemical methods. In a typical experiment, tumor fragments from seed mice inoculated with selected PDX tumors were harvested and used for inoculation of female Balb/c nude mice. Each mouse was inoculated subcutaneously at the right flank with one tumor fragment (2-3 mm in diameter) for tumor development. Treatments were started when mean tumor size reached approximately 200-250 mm3. Compound A (60-80 mg/kg) formulated as a suspension in 1% Kollidon VA64 in deionized water or vehicle alone (i.e., 1% Kollidon VA64 in deionized water) were administered orally by gavage to the tumor-bearing mice daily for 14 consecutive days.
Tumor volume was measured twice weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5 a×b2 where a and b are the long and short diameters of the tumor, respectively. Body weight was also recorded twice weekly. Treatment efficacy was expressed as ΔT/ΔC, whereby ΔT reports the relative change in tumor volume of drug-treated animals and ΔC the relative change in tumor volume of not-drug-treated animals between the last day of treatment and the beginning of treatment (median volume differences). Results obtained for the different models are shown in Table 2.
FGFR copy number was estimated by FISH. Tumor tissue obtained from tumor-bearing animals was fixed in buffered formalin and embedded in paraffin blocks (FFPE). Three to four μm tissue sections were mounted on silanized slides, deparaffinated, protease-treated, washed, DNA denatured and then hybridized to an FGFR probe as described by Schildhaus, H. U. et al. (2012) Mol. Pathol. 25: 1473-80. Subsequent to hybridization, tumor tissue was scanned for amplification hotspots using a fluorescent microscope as also described in Schildhaus et al. (2012). FGFR probes used were FGFR1/CEN 8 Dual Color Probe, FGFR2/CEN 10 Dual Color Probe and FGFR3/4p11 Dual Color Probe (all from Zytovision GmbH, Bremerhaven, Germany). Gene copy number gain (GCN) was defined as either amplification (FISH FGFR probe-centromere probe ratio ≥2.2) or polysomy, defined as FGFR probe-centromere probe ratio <2.2 but each one of FGFR and centromeres probes >2.
To determine FGFR mRNA levels, total RNA was extracted from one to six macro-dissected 10 μm thick FFPE sections using the QIAgen miRNeasy FFPE kit (QIAgen 217504) according to the manufacturer's instructions. The procedure involved lysis of the deparaffinized tissue using the QIAgen proprietary RNA tissue lysis buffer and incubation with Proteinase K. In the presence of chaotropic salts, the RNA was specifically bound to the glass fibers of a spin column. Bound RNA was incubated with DNase and purified in a series of rapid wash-and-spin steps and then eluted in water. RNA concentration was determined by absorbance using a NanoDrop spectrophotometer (Thermo Fischer Scientific, Waltham, Mass., USA) and fluorescence using Qubit fluorometer (Life Technologies).
300 ng of total RNA per sample were analyzed using the nCounter Gene Expression Assay protocol as instructed by the manufacturer NanoString Technologies Inc., Seattle, Wash., USA (www.nanostring.com). The nCounter assay is based on direct digital detection of mRNA molecules of interest using target-specific, color-coded probes that hybridize directly to the target molecules in solution, so that the expression level of each gene is measured in a relative fashion by counts, without the need for cDNA synthesis and amplification. Each probe is constituted of a reporter probe part of 50 bases that carries the barcode on the 5′ end and a capture probe part of 50 bases that carries a biotin molecule on the 3′ end allowing the target-probe complex to be immobilized on a streptavidin-coated nCounter Cartridge for digital data collection (Counts) after washout of excess probes. Descriptions of the NanoString methodology are found in Geiss, G. K. et al. (2008) Nat. Biotech. 26: 317-325; Malkow, V. A. et al (2009) BMC Research Notes 2: 80; and Guancial, E. A. et al. (2014) Cancer Med. 3(4): 835-44.
FGFR genes and reference (i.e. housekeeping) genes were selected by the inventors. Probes for such FGFR and housekeeping genes were designed and synthetized by NanoString Technologies Inc. (Custom CodeSet), then inserted with all consumables and reagents (including 8 negative and 6 positive normalization probes provided by NanoString) in a ready-to-use nCounter Master Kit for sample processing in the nCounter Analysis System. Negative normalization probes are used in the background correction (see below) and positive normalization probes are used for cartridge quality control only. The method of normalization using the positive normalization probes was that described in the nCounter Expression Data Analysis Guide published by NanoString in 2012. The target sequences (FGFR target sequences as well as target sequences for standardization, i.e. sequences of housekeeping genes) used for the design of probes, as provided by NanoString, are reproduced in Table 3 below. References for normalization probes are provided in Table 4 below. FGFR mRNA levels (expressed relative to those of a set of 16 reference genes consisting of ACTB, ALAS1, CLTC, MRPL19, RPL19, RPLP0, SF3A1, TBP, TUBB, c1orf43 exon 1, c1orf43 exon 2, CHMP2A exon 3, EMC7 exon 5, EMC7 exon 3, GPI exon 4 and GPI exon 6) determined for the different models are presented in Table 2.
Count values obtained were background-corrected. Background-corrected values were standardized based on parallel measurement of expression levels of “housekeeping genes” in each RNA specimen analyzed. The following procedure was used:
Background Correction
Housekeeping Gene Standardization
It is noted that normalized threshold values are sensitive to the reference gene set utilized for standardization (Table 5).
Alternatively, FGFR mRNA levels may be determined by any other method capable of quantitating reliably mRNA levels. Suitable methods include but are not limited to:
(1) Northern blots. Northern blots can be utilized to estimate the molecular weight of an mRNA and to measure relative amounts of mRNAs present in different samples. A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. RNA is separated by gel electrophoresis, usually on an agarose gel. Because there are so many different RNA species on the gel, RNA usually appears as a smear rather than discrete bands. The RNA is transferred to a nitrocellulose membrane, although other types of membranes can also be used. The RNA molecules retain the same pattern of separation they had in the gel. The blot is incubated with a probe, typically a single-stranded DNA. This probe will form base pairs with its complementary RNA sequence and bind to form a double-stranded RNA-DNA molecule. The probe may be radiolabeled or coupled to an enzyme (e.g., alkaline phosphatase or horseradish peroxidase). In the latter case, the location of the probe is revealed by incubating it with a colorless substrate that the attached enzyme converts to a colored product that can be seen or gives off light which will expose X-ray film. If the probe was labeled with radioactivity, it can expose X-ray film directly. (2000) Invest. Ophthalmol. Vis. Sci. 41: 2357-62.
(2) Nuclease protection assays (NPA). The NPA (including both ribonuclease protection assays and S1 nuclease assays) is an extremely sensitive method for the detection and quantitation of specific mRNAs. The extracted RNA is first mixed with radiolabeled RNA or DNA probes that are complementary to the sequence or sequences of interest and the complementary strands are hybridized to form double-stranded RNA (or DNA-RNA hybrid). The mixture is then exposed to ribonucleases that specifically cleave only single-stranded RNA (DNA) but have no activity against double-stranded RNA (or DNA-RNA hybrid). When the reaction runs to completion, susceptible RNA regions are degraded to very short oligomers or to individual nucleotides; the surviving RNA fragments are those that were complementary to the added antisense strand and thus contained the sequence of interest. The remaining protected fragments are separated by gel electrophoresis and visualized by autoradiography. Maddula, K. et al. FGFR and FGF ligand overexpression in lung cancer: Implications for targeted therapy. HTG Molecular Diagnostics, Tucson, Ariz., USA, 2014 ASCO Annual Meeting.
(3) Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RT-qPCR has revolutionized the study of gene expression. It is now theoretically possible to detect the RNA transcript of any gene, regardless of the scarcity of the starting material or the relative abundance of the specific mRNA. In RT-qPCR, an RNA template is copied into a complementary DNA (cDNA) using a retroviral reverse transcriptase. The cDNA is then amplified exponentially by PCR and the amplification is linked to the generation of fluorescence which can simply be detected with a camera during each PCR cycle. The process is monitored in “real-time” so is quantitative. Relative quantitative RT-qPCR involves amplifying an internal control transcript simultaneously with the gene transcript of interest. The internal control is used to standardize the samples. Once standardized, direct comparisons of relative abundance of a specific mRNA can be made across the samples. For relative RT-qPCR results to be meaningful, all products of the PCR reaction must be analyzed in the linear range of amplification. Yan, D., et al. (2011) Arthritis Res. Ther. 13(4): R130; Wong, M. L. and Medrano, J. F. (2005) BioTechniques 39: 75-85.
(4) Hybridization to DNA microarray. GeneChip microarrays use hybridization of DNA segments (on the array) and RNA target molecules (from the samples to be analyzed) to determine the expression level, i.e., how much RNA is being made from a given gene. Known segments of DNA are used as “probes” to bind and identify sample mRNA. Although this is not substantially different from other hybridization techniques (such as Southern and Northern blotting), what is unique about microarrays is the amount of information that is provided at one time. Tens of thousands of DNA sequences (or even larger numbers of oligonucleotides) are arranged in a known and orderly fashion (hence “array”) on a small solid support structure or microchip. The RNA sample after amplification and labeling is allowed to bind to the array. If a gene in the sample is expressed, its mRNA (cDNA) will hybridize to its complementary DNA probe on the chip. The array is then scanned, providing both qualitative and quantitative information about the function of specific genes. Dalma-Weiszhausz, D. D. et al. (2006) Methods Enzymol. 410: 3-28; Yadav, V. et al. (2012) J. Biol. Chem. 287: 28087-98.
(5) RNA-Seq. RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. All RNA-Seq experiments follow a similar protocol. Total RNA is isolated from a sample of interest which, depending on the type of RNA to be profiled, may be purified to enrich for mRNAs, microRNAs or lincRNAs, etc., prior to preparing a library. Library preparation may involve such steps as reverse transcription to cDNA, PCR amplification and may or may not preserve strandedness information. Sequencing can produce one read in a single-end sequencing reaction, or two ends separated by an unsequenced fragment in paired-end reactions. Li, F. et al. (2015) Cancer Discov. 5: 438-51. For a review of deep sequencing technologies, see Metzker, M. L. et al. (2010) Nat. Rev. Genet. 11: 31-46.
Expression of FGFR genes may also be assessed by methods that are capable of quantitating reliably the protein levels of different FGFRs. Suitable methods include but are not limited to:
(1) Western blotting. Western blotting uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with primary antibodies specific to the target protein. Once bound the antibody is visualized, either with a specific tag coupled to the primary antibody or with a secondary antibody that can be visualized. Wynes, M. W. et al. (2014) Clin. Cancer Res. 20: 3299-309.
(2) lmmunodot assay. Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool. The key feature of dot blotting is the use of immunodetection to identify a specific protein, for example a protein marker for a disease. Once proteins are immobilized on a protein-binding membrane, usually nitrocellulose or PVDF, they can be probed with a primary antibody, i.e., an antibody specific for the protein of interest. Once bound, the antibody is visualized either with a specific tag coupled to the primary antibody or with a secondary antibody. Girjes, A. A. et al. (1993) Veterinary Record 133: 136-41; Shekhar, M. S. (2010) Aquaculture Research 41: 1683-90.
(3) ImmunoAssay. The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance, and a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate. A number of platform technologies offer methods for multiplexed and miniaturized immunoaffinity assays (e.g., Luminex, MesoScale Discovery and PerkinElmer). Human fibroblast growth factor receptor 3 (FGFR3) ELISA Kit (http://www.cusabio.com/); Lequin, R. M. (2005) Clin. Chem. 51: 2415-8.
(4) Immunochromatography. Immunochromatography assay (also known as lateral flow or strip test) utilizes simple devices intended to detect the presence (or absence) of a target analyte in a sample (matrix) without the need for specialized and costly equipment, though many applications exist that are supported by reading equipment. The technology is based on a series of capillary beds, such as porous paper or sintered polymer. Each of these elements has the capacity to transport fluid (e.g., urine) spontaneously. The first element (the sample pad) acts as a sponge and holds an excess of sample fluid. Once soaked, the fluid migrates to the second element (conjugate pad), a dried format of bio-active particles in a salt-sugar matrix that contains everything to guarantee an optimized chemical reaction between the target molecule (e.g., an antigen) and its chemical partner (e.g., antibody) that has been immobilized on the particles' surface. While the sample fluid dissolves the salt-sugar matrix, it also dissolves the particles and in one combined transport action the sample and conjugate mix while flowing through the porous structure. In this way, the analyte binds to the particles while migrating further through the third capillary bed. This material has one or more areas (often called stripes) where a third molecule has been immobilized by the manufacturer. By the time the sample-conjugate mix reaches these strips, analyte has been bound on the particle and the third ‘capture’ molecule binds the complex. After a while, when more and more fluid has passed the stripes, particles accumulate and the stripe-area changes color. Typically, there are at least two stripes: one (the control) that captures any particle and thereby shows that reaction conditions and technology worked fine, the second contains a specific capture molecule and only captures those particles onto which an analyte molecule has been immobilized. After passing these reaction zones the fluid enters the final porous material, the wick that simply acts as a waste container. Zeng. Q. et al. (2009) Am. J. Biomed. Sci. 1:70-79.
(5) Immunohistochemistry. Immunohistochemistry (IHC) is a method capable of demonstrating the presence and location of proteins in tissue sections. Though being semi-quantitative only, it enables the observation of processes in the context of intact tissue. The basic steps of the IHC protocol are as follows: fixing and embedding the tissue, cutting and mounting the section, deparaffinizing and rehydrating the section, applying antigen retrieval process, immunohistochemical staining and viewing the staining under the microscope. The following protocol was used to detect FGFR expression in tumor tissues: immunostaining was performed on 4-μm paraffin-embedded tissue sections. Briefly, slides were deparaffinized in xylene and dehydrated utilizing a graded ethanol series, and endogenous peroxidase was blocked with 3% hydrogen peroxide. After epitope retrieval, the slides were washed with and blocked with TRIS-buffered saline with 0.1% (vol.) Tween 20/5% (vol.) normal goat serum. Incubation with the primary antibody was performed overnight at 4° C. followed by incubation with the secondary antibody for 30 min at room temperature. Sections were washed three times with TRIS-buffered saline with 0.1% (vol.) Tween 20, stained with diaminobenzidine (DAB) and counterstained with hematoxylin. Primary antibodies:
FGFR1 Rabbit monoclonal antibody (Cell Signaling Technology, Cat# 9740, clone #D8E4)
FGFR2 Rabbit polyclonal antibody (Novus Biologicals, Cat# NB200-642)
FGFR3 Mouse monoclonal antibody (Santa Cruz Biotechnology, Cat# sc-13121, clone B-9)
Guancial et al. (2014); Redler, A. et al. (2013) PLoS One. 8: e72224.
(6) Mass Spectrometry. Mass spectrometry (MS) measures the mass-to-charge ratio of ions to identify and quantify proteins. While mass spectrometry can detect very low analyte concentrations in complex mixtures, MS is not inherently quantitative because of the considerable loss of peptides and ions during analysis. Therefore, peptide labels or standards are concomitantly analyzed with the sample and act as a reference point for relative or absolute peptide quantitation. Catenacci. D. V. et al. (2014) PLoS One 9: e100586; Razavi, M. et al. (2013) Clin. Chem. 59:1514-22. A mass spectrometry approach using Liquid Tissue-Selected reaction monitoring (LT-SRM) can be used to objectively quantify the levels of FGFR. Hembrough, T. et al. (2012) Clin. Proteomics: 9: 5; Hembrough, T. et al. (2013) J. Mol. Diagn. 5:454-65.
(7) Flow Cytometry. Flow cytometry is a laser-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, involving suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second. Using antibody specific of protein, flow cytometry can provide information regarding the expression of cell surface and, in some cases, cytoplasmic or nuclear markers that are used to understand complex cellular populations or processes. Yan, D. et al. (2011) Arthritis Res.Ther. 13: R130.
A waterfall plot of the ΔT/ΔC values of all 66 PDX models is shown in
Surprisingly, when only models were considered that overexpressed an FGFR to a certain level, 21 of 40 PDX models (response rate of about 52%) were found to be responsive to Compound A (
A post-hoc analysis of all models identified the presence of FGFR mutations in some models. Calculation of the response rates including such mutations as a selection criteria surprisingly confirmed the superior predictive power of FGFR overexpression over genetic alterations. A waterfall plot of ΔT/ΔC values of all PDX models that exhibited a gene copy number gain and/or the presence of a FGFR fusion gene and/or an FGFR mutation revealed a response rate of about 32% (
It is noted that the predictive power of FGFR overexpression can be altered by decreasing or increasing the threshold FGFR expression levels. At the 44th percentile cutoff level of expression, the response rate to therapy with Compound A was about 36% (Table 1). This is still a better response rate than that produced by selection for an increased copy number of an FGFR gene and/or the presence of an FGFR fusion gene and/or the presence of an
FGFR mutation. At the 73th percentile cutoff level of expression, the response rate was considerably higher, i.e., over 52% (
The overexpression selection criterion was found to be relatively insensitive to the ΔT/ΔC threshold value that was chosen to separate effective from ineffective treatment by Compound A. In the above analysis, the ΔT/ΔC threshold was set at zero (0), corresponding to complete arrest of tumor growth during the treatment period. When the ΔT/ΔC threshold was set at 0.4, corresponding to substantial reduction of tumor growth during the treatment period, the response rate without selection was 51.5% (
With a ΔT/ΔC threshold set at 0.4, the same observation as above can be made when considering FGFR mutations in the selection criteria. When only models were considered that had an increased copy number of an FGFR gene, expressed an FGFR fusion gene and/or had a mutation in an FGFR gene, response rate was 52.4% (
As discussed previously, the above analyses were not limited by the methodology used to estimate FGFR expression. Analogous data could be produced by other methods of measuring mRNA levels such as Northern blots, nuclease protection assays, RT-qPCR, microarray hybridization or RNA-Seq. An example demonstrating that RT-qPCR and NanoString mRNA expression results are very well correlated is shown in
Overexpression of an FGFR is a successful criterion for the selection of subjects that are likely to respond to therapy with Compound A independent of cancer indication or subtype. Stratification of the analysis based on cancer indication or subtype has the potential of enhancing the predictive power of the overexpression analysis (that is carried out as described above) in particular indications or subtypes. Examples showing that the correlation between FGFR expression and treatment efficacy holds for particular indications are presented in
Based on the above-described findings and conclusions, the inventors propose a method for identifying and selecting subjects diagnosed with a cancer that are likely to respond to treatment with Compound A. The first step consists of obtaining from a subject a tumor tissue biopsy or a liquid biopsy (e.g., blood, from which circulating tumor cells or exosomes may be isolated). The biopsy is processed according to the requirements of the method chosen for determining levels of expression of FGFR1, FGFR2 and FGFR3 genes. In the next step, amounts of FGFR1, FGFR2 and FGFR3 RNA or protein are estimated. In the final step, the expression values so obtained are compared to the pre-established threshold values that were determined by analyses of the type discussed before. If a subject's level of RNA or protein for any of FGFR1, FGFR2 and FGFR3 exceeds the respective threshold value, the subject is considered a candidate for treatment with Compound A. The invention also relates to a method of personalized cancer therapy, in which a patient selected by the latter diagnostic method as being likely to respond to treatment with Compound A, is subjected to a therapeutic regimen involving administration of a pharmaceutical composition comprising Compound A.
It is noted that pre-established threshold levels for specific cancer indications and, consequentially, cut-off levels, may be different from those determined for all indications. For esophageal squamous-cell carcinoma, threshold FGFR1 levels corresponding to cutoff levels of from about 44% to about 84% (0.104-1.362) may be selected as this range of threshold levels does not exclude any model that is capable of responding to Compound A. More preferred are threshold FGFR1 levels corresponding to cutoff levels from about 60% and about 84% (0.301-1.362). Even more preferred are threshold FGFR1 levels corresponding to cutoff levels from about 70% and about 84% (0.558-1.362). Most preferred are threshold FGFR1 levels corresponding to cutoff levels from about 80% and about 84% (0.759-1.362). For gastric cancer, threshold FGFR2 levels corresponding to cutoff levels of from about 44% to about 83% (0.257-1.610) may be selected as this range of threshold levels does not exclude any model that is capable of responding to Compound A. More preferred are threshold FGFR2 levels corresponding to cutoff levels from about 60% and about 83% (0.669-1.610). Even more preferred are threshold FGFR2 levels corresponding to cutoff levels from about 70% and about 83% (0.984-1.610). Most preferred are threshold FGFR2 levels corresponding to cutoff levels from about 80% and about 83% (1.460-1.610). It is noted that in the general (indication-independent) method, the levels of FGFR1, FGFR2 and FGFR3 are determined, whereas in a method relating to a specific type of cancer, it is sufficient to determine the level of the FGFR(s) of interest (i.e. known to be elevated in tumors of a fractions of subjects suffering from a cancer of this type) only.
It is further noted that the disclosed diagnostic method relies on the rational assumption that a patient's tumor and a tumor grown in an animal directly from fragments of the patient's tumor exhibit closely similar levels of expression of FGFR genes at both RNA and protein levels. The plausibility of this assumption was borne out by a recent analysis by Guo et al. 2016 Cancer Res. [Epub ahead of print] (CAN-15-3245 Published June 2016).
Pharmaceutical compositions of the present invention comprising FGFR kinase inhibitor Compound A may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir, preferably by oral administration or administration by injection. The pharmaceutical compositions may contain any conventional non-toxic pharmaceutically acceptable carriers, adjuvants or vehicles. In some cases, the pH of the formulation may be adjusted with pharmaceutically acceptable acids, bases or buffers to enhance the stability of the active agent or its delivery form. Standard pharmaceutical carriers and their formulations are described, in a non-limiting fashion, in Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa., 19th ed. 1995. The term parenteral as used herein includes subcutaneous, intracutaneous, intravenous, intramuscular, intraarticular, intraarterial, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection or infusion techniques.
Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to active agent, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other emulsifiers, solubilizing agents and solvents such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions, may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride and Dextrose solutions. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.
The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, autoclaving, dry heat, ionizing radiation, or by incorporating active agent in the form of a sterile solid composition which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
In order to prolong the effect of the active agent, it is often desirable to slow the absorption of the active agent from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the active agent then depends upon its rate of dissolution, which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the active agent in an oil vehicle. Injectable depot forms are made by microencapsulating the active agent in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of active agent to polymer and the nature of the particular polymer employed, the rate of release of the active agent can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the active agent in liposomes or microemulsions that are compatible with body tissues.
Solid dosage forms for oral administration include capsules, tablets, pills, powders and granules. In such solid dosage forms, active agent is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or: a) fillers or extenders such as starches, lactose, cellulose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar-agar, calcium carbonate, croscarmellose, crospovidone, carboxymethylcellulise, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution-retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol, sodium lauryl sulfate and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and/or i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents.
Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active agent only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.
In general, treatment regimens according to the present invention comprise administration to a human subject in need of such treatment from 10 mg to 250 mg of Compound A per day in single or multiple doses, such as 2 daily doses.
The amount of active agent that may be combined with pharmaceutically acceptable excipients or carriers to produce a single dosage form will vary depending on the particular mode of administration and, possibly, on the subject treated. A typical preparation will contain from 1% to 95% active agent (w/w). Alternatively, such preparations may contain from 20% to 80% active agent. Lower or higher doses than those recited above may be required. Specific dosage and treatment regimens for any particular subject will depend upon a variety of factors, including the age, body weight, body surface area, general health status, sex, diet, time of administration, rate of excretion, drug combination, the severity and course of the disease, condition or symptoms, the subject's disposition to the disease, condition or symptoms, and the judgment of the treating physician.
Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. Unless otherwise stated, all exact values provided herein are representative of corresponding approximate values (e. g., all exact exemplary values provided with respect to a particular factor or measurement can be considered to also provide a corresponding approximate measurement, modified by “about,” where appropriate).
The use of any and all examples, or exemplary language (e.g., “such as”) provided herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise indicated.
The citation and incorporation of patent documents herein is done for convenience only and does not reflect any view of the validity, patentability and/or enforceability of such patent documents. The description herein of any aspect or embodiment of the invention using terms such as reference to an element or elements is intended to provide support for a similar aspect or embodiment of the invention that “consists of',” “consists essentially of” or “substantially comprises” that particular element or elements, unless otherwise stated or clearly contradicted by context (e. g., a composition described as comprising a particular element should be understood as also describing a composition consisting of that element, unless otherwise stated or clearly contradicted by context).
This invention includes all modifications and equivalents of the subject matter recited in the aspects or claims presented herein to the maximum extent permitted by applicable law.
All publications and patent documents cited in this specification are herein incorporated by reference in their entireties as if each individual publication or patent document were specifically and individually indicated to be incorporated by reference.
EXAMPLES
11 PDX models of gastric cancer were selected considering their GCN and level of expression of FGFR2 as well as FGFR2 fusions, so as to constitute a balanced set of models with FGFR2 expression levels as well distributed as possible.
For each tumor model, the effects of Compound A on tumor growth (treatment efficacy), FGFR copy number and FGFR mRNA levels were assessed (or re-assessed). Tumor fragments from seed mice inoculated with selected PDX tumors were harvested and used for inoculation of female Balb/c nude mice. Each mouse was inoculated subcutaneously at the right flank with one tumor fragment (2-3 mm in diameter) for tumor development. Treatments were started when mean tumor size reached approximately 200-250mm3. Compound A (60-80 mg/kg) formulated as a suspension in 1% Kollidon VA64 in deionized water or vehicle alone (i.e., 1% Kollidon VA64 in deionized water) were administered orally by gavage to the tumor-bearing mice daily for 14 consecutive days. For each PDX model, 3 mice were used in the treatment group and 3 mice were used as control animals (vehicle only).
Tumor volume was measured twice weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5 a×b2 where a and b are the long and short diameters of the tumor, respectively. Body weight was also recorded twice weekly. Treatment efficacy was expressed as ΔT/ΔC, whereby ΔT reports the relative change in tumor volume of drug-treated animals and ΔC the relative change in tumor volume of not-drug-treated animals between the last day of treatment and the beginning of treatment (median volume differences). Results obtained for the different models are shown in Table 6.
FGFR copy number was estimated by FISH. Tumor tissue obtained from tumor-bearing animals was fixed in buffered formalin and embedded in paraffin blocks (FFPE). Three to four μm tissue sections were mounted on silanized slides, deparaffinated, protease-treated, washed, DNA denatured and then hybridized to an FGFR probe as described by Schildhaus, H. U. et al. (2012) Mol. Pathol. 25: 1473-80. Subsequent to hybridization, tumor tissue was scanned for amplification hotspots using a fluorescent microscope as also described in Schildhaus et al. (2012). FGFR probes used were FGFR1/CEN 8 Dual Color Probe, FGFR2/CEN 10 Dual Color Probe and FGFR3/4p11 Dual Color Probe (all from Zytovision GmbH, Bremerhaven, Germany). Gene copy number gain (GCN) was defined as either amplification (FISH FGFR probe-centromere probe ratio ?.2.2) or polysomy, defined as FGFR probe-centromere probe ratio <2.2 but each one of FGFR and centromeres probes >2.
mRNA levels were determined by NanoString technology as described in the Methodology section above.
The relationship between treatment efficacy with Compound A (ΔT/ΔC) and FGFR2 mRNA expression (measured as expression level of one of the above-mentioned exons 10-14 and 16) are shown in
It clearly appears that responding models and non-responding models displayed different levels of FGFR2 expression, and that selecting models (i.e. patient tumors) on the basis of FGFR2 amplification only (empty circles) does not allow to select all responding models. Indeed, one model displaying no amplification but high level of FGFR2 expression responded to Compound A.
13 PDX models of ESCC were selected considering their GCN and level of expression of FGFR1, so as to constitute a balanced set of models with FGFR1 expression levels as well distributed as possible. In this particular ESCC indication, no FGFR1 fusions have been reported yet.
For each tumor model, the effects of Compound A on tumor growth (treatment efficacy), FGFR copy number and FGFR mRNA levels were assessed (or re-assessed). Tumor fragments from seed mice inoculated with selected PDX tumors were harvested and used for inoculation of female Balb/c nude mice. Each mouse was inoculated subcutaneously at the right flank with one tumor fragment (2-3 mm in diameter) for tumor development. Treatments were started when mean tumor size reached approximately 200-250 mm3. Compound A (60-80 mg/kg) formulated as a suspension in 1% Kollidon VA64 in deionized water or vehicle alone (i.e., 1% Kollidon VA64 in deionized water) were administered orally by gavage to the tumor-bearing mice daily for 14 consecutive days. For each PDX model, 3 mice were used in the treatment group arid 3 mice were used as control animals (vehicle only).
Tumor volume was measured twice weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5 a×b2 where a and b are the long and short diameters of the tumor, respectively. Body weight was also recorded twice weekly. Treatment efficacy was expressed as ΔT/ΔC, whereby ΔT reports the relative change in tumor volume of drug-treated animals and AC the relative change in tumor volume of not-drug-treated animals between the last day of treatment and the beginning of treatment (median volume differences). Results obtained for the different models are shown in Table 7.
FGFR copy number was estimated by FISH. Tumor tissue obtained from tumor-bearing animals was fixed in buffered formalin and embedded in paraffin blocks (FFPE). Three to four μm tissue sections were mounted on silanized slides, deparaffinated, protease-treated, washed, DNA denatured and then hybridized to an FGFR probe as described by Schildhaus, H. U. et al. (2012) Mol. Pathol. 25: 1473-80. Subsequent to hybridization, tumor tissue was scanned for amplification hotspots using a fluorescent microscope as also described in Schildhaus et al. (2012). FGFR probes used were FGFR1/CEN 8 Dual Color Probe, FGFR2/CEN 10 Dual Color Probe and FGFR3/4p11 Dual Color Probe (all from Zytovision GmbH, Bremerhaven, Germany). Gene copy number gain (GCN) was defined as either amplification (FISH FGFR probe-centromere probe ratio ≥2.2) or polysomy, defined as FGFR probe-centromere probe ratio <2.2 but each one of FGFR and centromeres probes >2.
mRNA levels were determined by NanoString technology as described in the Methodology section above.
The relationship between treatment efficacy with Compound A (ΔT/ΔC) and FGFR1 mRNA expression (measured as expression level of one of the above-mentioned exons 10-14) are shown in
It clearly appears that responding models and non-responding models displayed different levels of FGFR1 expression, and that for this particular ESCC indication, genetic structural alteration such as amplification (empty circles) does not allow to select models that respond to Compound A.
Although the present invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be readily apparent to one of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the scope of the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| PCT/IB2015/001245 | Jul 2015 | IB | international |
| PCT/IB2016/000290 | Mar 2016 | IB | international |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IB2016/001044 | 7/21/2016 | WO | 00 |
