Food waste treatment device using microorganisms

Abstract

The present invention is directed to a composition for decomposing a majority of food waste into water and carbon dioxide comprising an effective combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes or any combinations thereof. A preferred embodiment comprises a combination of four different species of microbes having DNA sequences that correspond to SEQ ID Nos. 5-8 and which were deposited with the Korean Collection for Type Cultures (KCTC) on Mar. 8, 2007, and designated KCTC11085BP; KCTC11086BP; KCTC11087BP; KCTC11088BP, respectively. Also, presented by this invention is a device and methods for decomposing a majority of food waste into water and carbon dioxide using the microbial compositions presented herein.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts the external appearance of the food waste treatment device of the present invention.

FIG. 2 depicts the internal structure of the food waste treatment device of the present invention.

FIG. 3 depicts the processing container of the food waste treatment device of the present invention.

FIG. 4 depicts the bearing installed on the stirring axis of the food waste treatment device of the present invention.

FIG. 5 depicts the temperature control and drainage of the food waste treatment device of the present invention.

FIG. 6 depicts the 16S rDNA sequence (SEQ ID NO. 1) of a preferred bacteria strain used in the microorganism mixture, designated “B1” in Table 1, as well as a listing of bacterium species having the greatest homology with respect to this partial sequence.

FIG. 7 depicts the 16S rDNA sequence (SEQ ID NO. 2) of a preferred bacteria strain used in the microorganism mixture, designated “B2” in Table 1, as well as a listing of bacterium species having the greatest homology with respect to this partial sequence.

FIG. 8 depicts the 16S rDNA sequence (SEQ ID NO. 3) of a preferred bacteria strain used in the microorganism mixture, designated “B3” in Table 1, as well as a listing of bacterium species having the greatest homology with respect to this partial sequence.

FIG. 9 depicts the 16S rDNA sequence (SEQ ID NO. 4) of a preferred bacteria strain used in the microorganism mixture, designated “B4” in Table 1, as well as a listing of bacterium species having the greatest homology with respect to this partial sequence.

FIG. 10 depicts the rDNA sequence (SEQ ID NO. 5) of a preferred bacteria strain used in the microorganism mixture.

FIG. 11 depicts the rDNA sequence (SEQ ID NO. 6) of a preferred bacteria strain used in the microorganism mixture.

FIG. 12 depicts the rDNA sequence (SEQ ID NO. 7) of a preferred bacteria strain used in the microorganism mixture.

FIG. 13 depicts the rDNA sequence (SEQ ID NO. 8) of a preferred bacteria strain used in the microorganism mixture.

FIG. 14 depicts a production flowchart for the preparation of the microbial mixture for use in the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The present invention is directed to a food waste treatment composition used, with or without a device also described by this invention, which utilizes a particular combination of microbes to decompose the majority of food waste material and convert that food waste into water, carbon dioxide and a small amount of sediment.

The microbial mixture of the present invention is prepared and mixed with the food waste material. Using the appropriate microbial mixture results in decomposition of most of the food waste into water and carbon dioxide. In some embodiments of the microbial mixture, greater than 55% of the solid food waste is decomposed. In a preferred embodiment, up to 97% of the solid food waste is decomposed.

In certain embodiments, the microbial mixture comprises from 1 to about 10 different strains of bacteria. In certain preferred embodiments, the microbial mixture comprises about two to four different strains of microbial species. In yet other preferred embodiments, the mixture comprises four (4) different stains of microbial species.

Because the microbial mixture of this invention is capable of decomposing about 97% of the food waste material, many of the issues associated with other methods of food waste removal and/or treatment are alleviated. By reducing up to 97% of the solid food waste, the problems associated with large amounts of solid waste remaining (as with, e.g., composting) is eliminated. Furthermore, with the generation of mostly water as a by-product, much of the foul odor associated with decomposing organic waste is eliminated, as is the noxious leachates that can result from other decomposition methods. Additionally, the presently described compositions and methods are not costly and eliminate that inefficiencies associated with incineration, dehydration or other mechanical means of eliminating organic waste.

The Microbial Mixture:

The microbes used in the compositions of the present invention may include species of bacillus, lactobacillus, burkholderia, actimomyces, yeast fungus, eumycetes, as well as combinations thereof. The mixture is capable of decomposing the majority of food waste materials, including protein, starch, grease and cellulose; thus, one or more of the microbes of the mixture must be capable of decomposing long chain structures which are inherent in fats and starches of food waste.

The choice of microbes to add to the mixture and the proportion in which they are added may depend upon the general chemical composition of the food waste intended to be decomposed. The particular mixture of microbes used in a mix may be altered to more efficiently decompose waste of a particular composition. So, for example, a mixture may contain more or less of a given species of microbe to accommodate waste that is known to have a greater protein, fat or carbohydrate content. Thus, by way of an example, a preferred embodiment of this invention comprises four strains of bacteria, designated “B1”, “B2”, “B3” and “B4”. Their partial (16S rDNA) DNA sequences are provided in FIGS. 6-9 and are designated as SEQ ID Nos. 1-4, respectively. In this exemplary embodiment, “B1” was chosen as the predominant strain because of its ability to decompose carbon-nitrogen series of organic matter, as well as its ability to decompose some fat. “B2” was added for its ability to decompose starches and fats which have very long chain molecular structures. The overall time for decomposition of food waste is shortened by the addition of “B2” and its ability to break the long-chain structures of food wastes like starches and fats.

Additionally, when choosing microbes for the mixture, stability, vitality, adaptability, safety and resolvability of the microbes is also taken into consideration. In light of these criteria, strains such as streptomyces sp., cellulosinicrobium funkei, brucella sp., arthrobacter sp. and paenibacillus cookie may also be useful in the microbial mixture.

The mixture of the invention may contain anywhere from 1 to 10 strains of bacillus, lactobacillus, burkholderia, actimomyces, yeast fungus, eumycetes, as well as combinations thereof.

In a more preferred embodiment, the microbial composition is a mixture of three different bacillus species and one lactobacillus species. In a most preferred embodiment, the three bacillus species have DNA sequences corresponding to SEQ ID No. 5, SEQ ID No. 6 and SEQ ID NO. 8 and the lactobacillus species has a DNA sequences corresponding to SEQ ID No. 7.

In certain other preferred embodiments, the invention is a mixture of three different bacillus strains and one burkholderia strain, with: about 85% of a bacillus subtilis species, designated as “B1” in Table 1; about 12% of a second bacillus subtilis species, designated as “B2” in Table 1; about 1.5% of a third bacillus subtilis species, designated as “B4” in Table 1; and about 1.5% of a burkholderia species, designated as “B3” in Table 1:

TABLE 1
A Preferred Embodiment of Microbial Mixture
			%
	%		Similarity
	Composition	Closest Known Strains Based Partial Sequence	to Closest
Identifier	in Mixture	(16S rDNA Sequence)	Strains
B1	85%	MD 1979 (B4)	100.00
(SEQ ID		Bacillus subtilis subsp. subtilis DSM 10T	99.88
No. 1)		Bacillus subtilis subsp. spizizenii NRRL B-23049T	99.64
		Bacillus mojavensis IFO 15718T	99.64
		MD 1979 (B2)	99.53
		Bacillus atrophaeus JCM 9070T	99.41
		Bacillus amyloliquefaciens ATCC 23350T	99.40
		Bacillus vallismortis DSM 11031T	99.29
		Bacillus velezensis LMG 22478T	99.17
		Bacillus licheniformis DSM 13T	97.98
		Bacillus pumilus NCDO 1766T	95.85
		Bacillus carboniphilus JCM 9731T	95.36
		Bacillus oleronius ATCC 700005T	94.66
		Bacillus sporothermodurans DSM 10599T	94.54
		Bacillus firmus IAM 12464	94.38
		Bacillus indicus Sd/3T	94.10
		Bacillus azotoformans ATCC 29788T	93.88
		Bacillus methanolicus NCIMB 13114T	93.82
		Bacillus azotoformans DSM 1046T	93.71
		Bacillus badius ATCC 14574T	93.52
		Bacillus thuringiensis IAM 12077T	93.35
		Bacillus smithii DSM 4216T	93.27
		Bacillus cereus IAM 12605T	93.25
B2	12%	Bacillus amyloliquefaciens ATCC 23350T	99.76
(SEQ ID		Bacillus atrophaeus JCM 9070T	99.64
No. 2)		Bacillus vallismortis DSM 11031T	99.53
		Bacillus subtilis subsp. subtilis DSM 10T	99.41
		Bacillus velezensis LMG 22478T	99.41
		Bacillus mojavensis IFO 15718T	99.17
		Bacillus subtilis subsp. spizizenii NRRL B-23049T	99.17
		Bacillus licheniformis DSM 13T	97.75
		Bacillus pumilus NCDO 1766T	96.22
		Bacillus carboniphilus JCM 9731T	95.12
		Bacillus indicus Sd/3T	94.47
		Bacillus oleronius ATCC 700005T	94.43
		Bacillus sporothermodurans DSM 10599T	94.31
		Bacillus firmus IAM 12464	94.27
		Bacillus azotoformans ATCC 29788T	93.77
		Bacillus methanolicus NCIMB 13114T	93.59
		Bacillus thuringiensis IAM 12077T	93.36
		Bacillus mycoides DSM 2048T	93.29
		Bacillus cereus IAM 12605T	93.25
B3	1.5%	Burkholderia multivorans LMG 13010T	99.88
(SEQ ID		Burkholderia cenocepacia LMG 16656T	99.29
No. 3)		Burkholderia anthina R-4183T	99.17
		Burkholderia cepacia ATCC 25416T	99.17
		Burkholderia vietnamiensis LMG 10929T	99.05
		Burkholderia stabilis LMG 14294T	99.05
		Burkholderia pyrrocinia LMG 14191T	98.91
		Burkholderia ubonensis GTC-P3-415T	98.91
		Burkholderia ambifaria LMG 19182T	98.69
		Burkholderia glumae LMG 2196T	98.21
		Burkholderia gladioli ATCC 10248T	98.21
		Burkholderia plantarii LMG 9035TT	97.98
		Burkholderia pseudomallei 1026b	96.67
		Burkholderia sordidicola S5-BT	96.55
		Burkholderia mallei ATCC 23344T	96.43
		Burkholderia thailandensis E264T	96.31
		Burkholderia glathei ATCC 29195T	95.33
		Burkholderia sacchari LMG 19450T	95.25
		Burkholderia andropogonis ATCC 23061T	95.23
		Burkholderia phenazinium ATCC 33666T	94.72
		Burkholderia caryophylli ATCC 25418TT	94.65
		Burkholderia caledonica LMG 19076T	94.53
		Burkholderia fungorum LMG 16225T	94.05
		Burkholderia caribensis MWAP64T	93.68
		Burkholderia graminis C4D1M (type strain)T	93.58
		Burkholderia kururiensis JCM 10599T	93.34
B4	1.5%	Bacillus subtilis subsp. subtilis DSM 10T	99.88
(SEQ ID		Bacillus mojavensis IFO 15718T	99.64
No. 4)		Bacillus subtilis subsp. spizizenii NRRL B-23049T	99.64
		MD 1979-B2	99.53
		Bacillus subtilis BFAS	99.41
		Bacillus atrophaeus JCM 9070T	99.41
		Bacillus amyloliquefaciens ATCC 23350T	99.40
		Bacillus vallismortis DSM 11031T	99.29
		Bacillus velezensis LMG 22478T	99.17
		Bacillus licheniformis DSM 13T	97.99
		Bacillus pumilus NCDO 1766T	95.86
		Bacillus carboniphilus JCM 9731T	95.36
		Bacillus oleronius ATCC 700005T	94.67
		Bacillus sporothermodurans DSM 10599T	94.54
		Bacillus firmus IAM 12464	94.38
		Bacillus indicus Sd/3T	94.11
		Bacillus azotoformans ATCC 29788T	93.89
		Bacillus methanolicus NCIMB 13114T	93.83
		Bacillus badius ATCC 14574T	93.53
		Bacillus thuringiensis IAM 12077T	93.36
		Bacillus smithii DSM 4216T	93.28
		Bacillus cereus IAM 12605T	93.25
Note:
While B1 and B4 demonstrate the 16S rDNA sequence, they appear differently shaped under a microscope.

In a most preferred embodiment, the microbial mixture comprises about 85% of a bacillus species the DNA sequence of SEQ ID NO. 5; about 12% of a second bacillus species the DNA sequence of SEQ ID NO. 6; about 1.5% of a third bacillus species having the DNA sequence of SEQ ID NO. 8; and about 1.5% of a lactobacillus species having the DNA sequence of SEQ ID NO. 7. In this embodiment, the bacillus species having the DNA sequence of SEQ ID NO. 5 was chosen as the predominant strain because of its ability to decompose carbon-nitrogen series of organic matter, as well as its ability to decompose some fat. The bacteria having the DNA sequence of SEQ ID No. 6 was added for its ability to decompose starches and fats which have very long chain molecular structures. The overall time for decomposition of food waste is shortened by the addition of the bacteria with SEQ ID No. 6 and its ability to break the long-chain structures of food wastes like starches and fats. This microbial mixture (being a combination of microbes having the DNA sequences shown in SEQ ID Nos. 5-8) was deposited with the Korean Collection for Type Cultures (KCTC) on Mar. 8, 2007; these four species were designated KCTC11085BP (SEQ ID No. 5), KCTC11086BP (SEQ ID No. 6), KCTC11087BP (SEQ ID No. 7) and KCTC11088BP (SEQ ID No. 8), by the KCTC.

The bacteria strains used in the present invention may be artificially made or naturally occurring.

Preferred species of bacillus include amyloliquefaciens, subtilis, subtilis subsp. subtilis, subtilis subsp. spizizenii, mojavensis, atrophaeus, vallismortis, velezensis, licheniformis, pumilus, carboniphilus, oleronius, sporothermodurans, firmus, indicus, azotoformans, methanolicus, badius, thuringiensis, smithii, cereus, mycoides and combinations thereof.

Preferred species of lactobacillus include, for example, lactobacillus acidophilus, lactobacillus plantarum, lactobacillus brevis, lactobacillus sakei subsp. sakei, lactobacillus brevis, lactobacillus delbrueckii subsp. bulgaricus, lactobacillus casei, lactobacillus delbrueckii, lactobacillus fermentum, lactobacillus helveticus, lactobacillus plantarum, lactobacillus reuteri, lactobacillus sanfranciscensis and combinations thereof.

Preferred strains of burkholderia include multivorans, cenocepacia, anthina, vietnamiensis, stabiles, pyrrocinia, ubonensis, ambifaria, glumae, gladioli, plantarii, pseudomallei, sordidicola, mallei, thailandensis, glathei, sacchari, andropogonis, phenazinium, caryophylli, caledonica, fungorum, caribensis, graminis, kuruiensis and combinations thereof.

Optionally, the microbial mixture is added to a “filler” material. This filler, sometimes also referred to as a “moisture controller” in this disclosure, provides an environment in which the microbes can thrive and reproduce. The filler material of the present invention may be husks (such as chaff or rice hulls), wood chips or synthetic materials such as PolyEster. In a preferred embodiment, cedar wood chips are used as the filler.

When using a filler, the filler is soaked in the microbial mixture. The filler serves the additional purpose of maintaining the original concentration of microbes added to a food mixture. The microbes reside in, or on, the solid filler material and therefore, decrease the risk of microbes being “flushed” out with the water by-product of the invention.

When using filler, up to 40% of a container volume of a food waste treatment device (an example of such a device is described hereinbelow) may be consumed by the microbial soaked filler. The filler material needs minimal replacement in the device and provides space for microorganisms to thrive and reproduce. For example, when treating approximately 100 lbs of food waste per day, the microbial mixture was found to be effective for approximately 1 year—after which the microorganisms must be re-inoculated. The used filler material, when using husks or wood chips, may be used as fertilizer.

A very small amount of yeast fungus and eumycetes reside naturally in some filler material, e.g., wood chips. However, in a bacillus-predominant environment of some of the preferred embodiments of the microbial mixture, the eumycetes are unable to produce spores, and therefore comprise less than about 1% of the microbial mixture.

In preferred embodiments, the present invention provides a composition for decomposing a majority of food waste into water and carbon dioxide comprising an effective combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes or any combinations thereof. In more preferred embodiments, at least one of the strains of microorganisms is capable of decomposing long chain starches. In other preferred embodiments, the combination of microorganisms comprises at least one species of bacillus and at least one species of lactobacillus, and in more preferred embodiments, the combination of microorganisms comprises four species of microorganisms. In most preferred embodiments, each of the four species of microorganisms has a DNA sequence of SEQ ID No. 5, SEQ. ID No. 6, SEQ. ID No. 7 and SEQ. ID No. 8., and may comprise at least 85% SEQ ID No. 5, about 12% SEQ ID No, about 1.5% SEQ ID No. 7 and about 1.5% SEQ ID No. 8.

In preferred embodiments, the composition further comprises a filler material. In more preferred embodiments, the filler material is a husk, a wood chip (e.g., cedar wood chips), a man-made filler or combinations thereof.

In preferred embodiments, the composition as described herein, decomposes greater than 55% of the food waste, and in most preferred embodiments, about 97% of the food waste is decomposed.

In certain embodiments, the present invention provides a method for decomposing greater than about 55% of food waste material into water and carbon dioxide comprising: (i) adding an effective amount of a combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes and any combinations thereof; and (ii) mixing the food waste material and the combination of microorganisms for a time sufficient to allow the combination of microorganisms to decompose a majority of the food waste into water and carbon dioxide.

In preferred embodiments, greater than about 97% of the food waste material is decomposed by the above method.

In preferred embodiments, the combination of microorganisms used in the above method comprises at least one species of bacillus and at least one species of lactobacillus. In preferred embodiments, at least one of the microorganisms is capable of decomposing long chain starches. In more preferred embodiments, the combination of microorganisms comprises four species of microorganisms, and in even more preferred embodiments, the four species of microorganisms has a DNA sequence of SEQ ID No. 5, SEQ. ID No. 6, SEQ. ID No. 7 and SEQ. ID No. 8, and may comprise at least 85% having SEQ ID No. 5, about 12% having SEQ ID No. 6, about 1.5% having SEQ ID No. 7 and about 1.5% having SEQ ID No. 8.

In preferred embodiments, the above method further comprises soaking a filler material with the elective combination of microorganisms. In more preferred embodiments, the filler material is a husk, a wood chip (e.g., a cedar wood chip), a man-made filler or combinations thereof.

In preferred embodiments, 1 gram of the elective combination of microorganisms is mixed with about 110 kilograms of food waste material.

Also provided by this invention is a method of making the microbial mixture. Procedures for generating and preserving microbial mixtures are known in the art. Preferably, the microbial mixture of the present invention is formulated through lyophilization, which maximizes the number and the vitality of the microbes. FIG. 10 is a flow chart showing the procedures used to make the microbial mixture of the present invention; a detailed description is also provided in Example 1 hereinbelow. During lyophilization and pulverization, a protectant may be used. The protectant may be any known pasteurized composition, such as maltodextrin, trehalose, glucose or skim milk. This list is not exhaustive, as one of skill in the art would recognize other pasteurized compositions which would be useful in the present invention.

The Food Waste Treatment Device:

The food waste treatment device of the present invention may be any device capable providing maximum contact of the microbial mixture with the food waste and a drainage opening to filter out the water which results from the decomposition of food waste. The inventors of the present invention have created a device, as depicted in FIGS. 1-5, which provides optimal results when utilized with the microbial mixture of the present invention.

The figures in the drawings show the subject matter of the invention highly schematically and should be understood as not being to scale. The individual components of the subject matter according to the invention are represented so that their structure can be clearly shown.

FIG. 1 shows the main body (110) of the food waste treatment device which contains the processing container (130) which decomposes the food waste with microorganisms. The cover (120) is located at upper part of the main body. Hinge joints are used for cover (120). The main body (110) has wheels (112) attached to the bottom for ease of mobility. The opening of main body has a sensor (121) so the machine only operates when cover is completely closed. The control panel (200) is located in the front of the main body (110).

FIG. 2 shows the internal structure of the food waste treatment device. A filtering screen (135) is placed at the bottom of the processing container (131). In order to maximize the efficiency, it is recommended to use two or three stirrer arms (142). A driving motor (146) rotates the axis of the stirrer arms (142).

FIG. 3 shows the processing container (130). There are one or more “U” shape stirrer arms (142) on the outer surface of the stirring axis (141). In order to maximize the efficiency, it is recommended to use two or three stirrer arms (142). The stirrer (140) is located crossways within the processing container (130). One end of the stirrer (140) is fixed to the side of processing container (130) and has a driving motor to rotate the stirring axis (141). One end of stirrer arm (142) is fixed to the stirring axis (141) and the other end of the stirrer arm (142) is fixed to the center of the stirrer axis (141). At this point, it is recommended to arrange each arm at least 90 degrees tilted. The stirrer arm and outer surface of stirring axis have sharp blades attached (143 and 144, respectively).

Blades of the stirrer arm (143) beak the food waste that is in the processing container (130) and the axis blades (144) break the food waste that is entangled in the stirring axis (141)

A small amount of food waste may not be broken down, however, the pressure from the have load pushes the waste the food is then broken down by the axis blades (144).

In preferred embodiments of the present invention, at least one of the stirrer arms is “U” shaped. In more preferred embodiments, at least one of the stirrer arms is located at the outer surface of a stirring axis. In certain preferred embodiment the stirrer arm is arranged in at least a 90 degree angle. In other preferred embodiments, the center of at least one stirrer arm consists of a sharp cutter. In other preferred embodiments, either side of the stirring axis comprises a blade which is located across the stirring axis.

In preferred embodiments of the present invention, the device further comprises bearings located on both side of the stirring axis. In preferred embodiments, the bearings comprise a cap and a bushing. The cap is mounted on the inner wall of the processing container and the bushing is inserted at the inner center of the cap to pierce through the stirring axis. In certain embodiments, the bearings are made of non-metallic material to prevent corrosion and rust due to salinity contained by food waste.

In preferred embodiments, there is drainage that stores decomposed food wastes on the lower part of discharge outlet. In such an embodiment, one end of drainage may be connected with sewerage and the other end may be connected with a water supply to wash off remaining food wastes. In more preferred embodiments, sufficient pitch is provided to the side that is connected with sewerage.

FIG. 4 shows the bearings (147) installed on the stirring axis (141). Both ends of the stirring axis (141) are installed to the side of processing container (131) to allow for rotation. One end of stirring axis (141) penetrates processing container (130) and connects with the motor which is located on the outer surface of the processing container (130).

Both ends of stirrer axis (141) have bearings (147) for easier rotation of axis. Bearings that are used for the axis are preferably made of non-metallic material to prevent corrosion and rust due to salinity contained by food waste.

Furthermore, bearings (147) used at the each end of the stirring axis (141) consist of a cap (148) that is mounted on the inner wall of processing container (130) and a bushing (149), which is inserted at the inner center of the cap (148) to penetrate the stirring axis (141). A Packing gasket is placed to prevent moisture from food waste.

A temperature control (150) is installed at the outer wall of the processing container (130). Thin plates of heating element units (152) are placed for temperature control. The heating element units (152) are wrapped by insulation (154), and it surrounds the processing container (130). The processing container (130) has its own temperature sensor to keep the certain temperature.

FIG. 5 depicts the temperature control and drainage of the food waste treatment device. The moisture control (160) is installed at the upper part of processing container (130). It consists of nozzles (162) to check the moisture level of processing unit.

A water discharge is installed to wash off food waste from processing container that was filter through the filter screen (135). The water discharge consists of a discharge outlet (180) a drainage (181) is located at the lower part of discharge outlet (180). One end of drainage is connected with sewerage. One end of sewerage is connecting to the solenoid valve (190) which has water spray nozzles (182) for drainage (181).

The solenoid valve (190) supplies water to the nozzles (182), moisture control (160) and water discharge (180). This device may restrain the activities of microorganisms because of lack of oxygen supply if the cover is closed. Therefore, oxygen supply is one of the most important elements to maximize the activities of the microorganisms. A ventilation fan, also referred to as an oxygen supplier (170), is located on top of the inner walls of processing container (130).

The control panel (200) controls the main body (110), stirrer (140), temperature control (150), moisture control (160), oxygen supplier (170) and water discharge (180) with appropriate settings for time and temperature. In preferred embodiments, the humidity of the processing container is maintained at about 65% to about 75%, and the temperature of the processing container is maintained at about 20° C. to about 35° C.

To give further understanding of this device, the stirrer axis (141) rotates when the power is on (Timer: Approx. 5 min.) Then water is sprayed into the processing container (Solenoid valve timer: Approx. 30 Min.), which is controlled by moisture control (160). Broken down organisms will filter through the filter screen (135) and water discharge (180). Water spray nozzles will wash off drainage and transfer the broken organisms to sewage. After the process of the water spray nozzles are completed, the stirrer will stop itself. The process of break down will begin by microorganisms. When all of the processes are completed, the system will repeat itself by returning to the first step.

In certain preferred embodiments, the present invention is directed to a device for decomposing a majority of food waste material into water and carbon dioxide comprising: a processing container for receiving food waste material, having sides, a bottom and a lid and containing a composition for decomposing a majority of food waste material into water and carbon dioxide comprising an effective combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes or any combinations thereof; a stirring axis having a first end and a second end, the first end being attached to a side of the processing container and the second end being attached to an opposite side of the processing container; at least one stirrer arm attached to the stirring axis; a filter screen located near the bottom of the processing container; and a water discharge outlet connected to the processing container and located opposite the filter screen.

In more preferred embodiments, the device further comprises a control panel attached to the processing container for controlling the speed of the stirring axis, temperature, and humidity inside the processing container. In more preferred embodiments, the humidity in the processing container of the device is maintained at about 65% to about 75% and the temperature in the processing container is maintained at about 20° C. to about 35° C. In even more preferred embodiments, the control panel further controls oxygen supply to the processing container.

In preferred embodiments, the stirrer arm of the device is bladed. In other preferred embodiments, the stirrer arm is U-shaped.

In certain preferred embodiments, at least one of the species of microorganisms placed into the processing container is capable of decomposing long chain starches. In certain preferred embodiments, one of the species of microorganisms is eumycetes. In other preferred embodiments, the combination of microorganisms in the device comprises at least one species of bacillus and at least one species of lactobacillus. In more preferred embodiments, the combination of microorganisms comprises four species of microorganisms. In even more preferred embodiments, each of the four species of microorganisms has a DNA sequence of SEQ ID No. 5, SEQ. ID No. 6, SEQ. ID No. 7 and SEQ. ID No. 8, and may comprise at least 85% SEQ ID No. 5, about 12% SEQ ID No. 6, about 1.5% SEQ ID No. 7, and about 1.5% SEQ ID No. 8.

In certain embodiments, the processing container of the device further contains a filler material. In preferred embodiments, the filler material is a husk, a wood chip (e.g., cedar wood chips), a man-made filler or combinations thereof, and in most preferred embodiments, the filler material occupies up to about 40% of the volume of the processing container.

In preferred embodiments, the device is capable of decomposing greater than about 55% of the food waste, and in even more preferred embodiments, the device is capable of decomposing greater than about 97% of the food waste.

EXAMPLES

Example 1

Preparation of Microbial Mixture

Microbes Used: Bacillus subtilis (3 Kinds) and Lactobacillus

TABLE 2
Culture Medium
RAW MATERIAL     %
Peptone          2
Yeast Extract    1
Glucose          2
Sodium Acetate   0.1
Ammonium citrate 0.1
Sodium carbonate 0.05
K2HPO4           0.1
MgSO4            0.01
MnSO4            0.005
ZnSO4            0.001

1% of each spawn was inoculated to the culture medium. The culture medium, set forth in Table 2 (above), was added in the order of peptone, yeast extract and glucose to the culture fluid and dissolved completely at 140° F. Culture medium was pasteurized for at least 15 minutes at about 250° F. at 1.2 hPa, with no negative air pressure, and a pressure higher than 0.5 vvm of positive air when cooling after pasteurization was complete, maintaining constant water volume throughout the process. The volume of water used was the same before and after pasteurization of the culture medium.

Microbes were cultivated via depths nurture method (air supply and stirring) for 18-30 hours at 77° F.-98.6° F. and a pH of between about 6.0 and 7.0.

After cultivation process, microbes were collected by standard collection procedures, such as in a continuance centrifugal separator.

Lyophilization Method:

The collected body of microbes was mixed with an appropriate portion of protectant (such as maltodextrin, trehalose, glucose or skim milk). Lyophilization was conducted by quick freezing the mixture for 24 hours at −40° F., then placing into a lyophilizer for 3-4 days, using a shelf temperature of 86° F., a cold trap condenser below −94° F. and a vacuum below 15 mTorr. The dried microbes were homogenously pulverized using 100 μl strainer. The primary powder of the microbes was then vacuum packed and stored at 39.2° F.

The primary mixture of the above microbes may be added to the device with a suitable portion (e.g. about 40-70%) of a filler material. The filler material may be, for example, wooden chips such as cedar tree chips or oak tree chips. The filler material increases the efficiency and the rate of fermentation of the microbes by maximizing its contact area with food waste. The number of microbes is maintained at approximately 10^6-9 cfu/g.

Example 2

Mixing with Filler Material and Production of Final Product

The primary powder of the microbe mixture (made in Example 1) was mixed with husks (the filler material) and protectant in the following ratio: 0.03527 ounces primary powder (approximately 1 gram), 0.67 ounces protectant, 2.2 pounds husks. This proportion was prepared for a 250 pound (or approximately 110 kilograms) daily capacity of food waste. The primary powder/filler material/protectant mixture was placed into a mixer and mixed for 30 to 60 minutes. The final product was packed and stored in cool dark place at room temperature.

Example 3

Breakdown of Food Waste

A food waste treatment device was prepared using the microbial mixture of Example 2. 40% of the volume of the container of the device was filled with the husks of Example 2. The husks were wet with enough water to inoculate the microorganisms. After 3-4 minutes, food waste was placed in the device. At this time, the microorganisms were activated and started to break down the waste, then discharged the water through the filter screen.

In the preceding specification, the invention has been described with reference to specific exemplary embodiments and examples thereof. It will, however, be evident that various modifications and changes may be made thereto without departing from the broader spirit and scope of the invention as set forth in the claims that follow. The specification and drawings are accordingly to be regarded in an illustrative manner rather than a restrictive sense.

Claims

1. A composition for decomposing a majority of food waste into water and carbon dioxide comprising an effective combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes or any combinations thereof.

2. The composition of claim 1, wherein the majority of food waste decomposed is greater than 55% of the food waste.

3. The composition of claim 2, wherein the majority of food waste decomposed is about 97% of the food waste.

4. The composition of claim 3, wherein the combination of microorganisms comprises at least one species of bacillus and at least one species of lactobacillus.

5. The composition of claim 4, wherein the effective combination of microorganisms comprises four species of microorganisms.

6. The composition of claim 5, wherein the each of the four species of microorganisms has a DNA sequence of SEQ ID No. 5, SEQ. ID No. 6, SEQ. ID No. 7 and SEQ. ID No. 8.

7. The composition of claim 6, comprising at least 85% SEQ ID No. 5.

8. The composition of claim 6, comprising about 12% SEQ ID No. 6.

9. The composition of claim 6, comprising about 1.5% SEQ ID No. 7.

10. The composition of claim 6, comprising about 1.5% SEQ ID No. 8.

11. The composition of claim 1, wherein at least one of the strains of microorganisms is capable of decomposing long chain starches.

12. The composition of claim 1, further comprising a filler material.

13. The composition of claim 12, wherein the filler material is a husk, a wood chip, a man-made filler or combinations thereof.

14. The composition of claim 13, wherein the wood chip is a cedar wood chip.

15. The composition of claim 12, wherein one of the microorganism species is a species of eumycetes.

16. A device for decomposing a majority of food waste material into water and carbon dioxide comprising: a processing container for receiving food waste material, having sides, a bottom and a lid and containing a composition for decomposing a majority of food waste material into water and carbon dioxide comprising an effective combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes or any combinations thereof;a stirring axis having a first end and a second end, said first end being attached to a side of the processing container, said second end being attached to an opposite side of the processing container;at least one stirrer arm attached to the stirring axis;a filter screen located near the bottom of the processing container;a water discharge outlet connected to the processing container and located opposite the filter screen.

17. The device of claim 16, further comprising a control panel attached to the processing container for controlling the speed of the stirring axis, temperature, and humidity inside the processing container.

18. The device of claim 17, wherein the control panel further controls oxygen supply to the processing container.

19. The device of claim 16, wherein the stirrer arm is bladed.

20. The device of claim 19, wherein the stirrer arm is U-shaped.

21. The device of claim 17, wherein the humidity in the processing container of the device is maintained at about 65% to about 75%.

22. The device of claim 17, wherein the temperature in the processing container is maintained at about 20° C. to about 35° C.

23. The device of claim 16, wherein the combination of microorganisms comprises at least one species of bacillus and at least one species of lactobacillus.

24. The device of claim 24, wherein the effective combination of microorganisms comprises four species of microorganisms.

25. The device of claim 24, wherein the each of the four species of microorganisms has a DNA sequence of SEQ ID No. 5, SEQ. ID No. 6, SEQ. ID No. 7 and SEQ. ID No. 8.

26. The device of claim 25, comprising at least 85% SEQ ID No. 5.

27. The device of claim 25, comprising about 12% SEQ ID No. 6.

28. The device of claim 25, comprising about 1.5% SEQ ID No. 7.

29. The device of claim 25, comprising about 1.5% SEQ ID No. 8.

30. The device of claim 16, wherein at least one of the species of microorganisms is capable of decomposing long chain starches.

31. The device of claim 16, wherein the device is capable of decomposing greater than about 55% of the food waste.

32. The device of claim 16, wherein the device is capable of decomposing greater than about 97% of the food waste.

33. The device of claim 16, wherein the processing container further contains a filler material.

34. The device of claim 32, wherein the filler material is a husk, a wood chip, a man-made filler or combinations thereof.

35. The device of claim 33, wherein the wood chip is a cedar wood chip.

36. The device of claim 32, wherein the filler material occupies up to about 40% of the volume of the processing container.

37. The device of claim 34, wherein one of the species of microorganisms is eumycetes.

38. A method for decomposing greater than about 55% of food waste material into water and carbon dioxide comprising: (i) adding an effective amount of a combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes and any combinations thereof;(ii) mixing the food waste material and the combination of microorganisms for a time sufficient to allow the combination of microorganisms to decompose a majority of the food waste into water and carbon dioxide.

39. The method of claim 38, wherein the greater than about 97% of the food waste material is decomposed.

40. The method of claim 31 wherein the combination of microorganisms comprises at least one species of bacillus and at least one species of lactobacillus.

41. The method of claim 40, wherein the effective combination of microorganisms comprises four species of microorganisms.

42. The method of claim 41, wherein the each of the four species of microorganisms has a DNA sequence of SEQ ID No. 5, SEQ. ID No. 6, SEQ. ID No. 7 and SEQ. ID No. 8.

43. The method of claim 42, comprising at least 85% SEQ ID No. 5.

44. The method of claim 42, comprising about 12% SEQ ID No. 6.

45. The method of claim 42, comprising about 1.5% SEQ ID No. 7.

46. The method of claim 42, comprising about 1.5% SEQ ID No. 8.

47. The method of claim 38, wherein at least one of the microorganisms is capable of decomposing long chain starches.

48. The method of claim 38, further comprising soaking a filler material with the elective combination of microorganisms.

49. The method of claim 48, wherein the filler material is a husk, a wood chip, a man-made filler or combinations thereof.

50. The method of claim 48, wherein the wood chip is a cedar wood chip.

51. The method of claim 38, wherein 1 gram of the elective combination of microorganisms is mixed with about 110 kilograms of food waste material.