Claims
- 1. A composition comprising complementary fragments of a protein, said fragments generating an optically detectable signal when associated.
- 2. The composition of claim 1 wherein said fragments generate a fluorescent signal when associated.
- 3. The composition of claim 1 wherein said fragments generate a luminescent signal when associated.
- 4. The composition of claim 1 wherein said fragments generate a phosphorescent signal when associated.
- 5. The composition of claim 1 wherein said fragments are derived from a fluorescent protein.
- 6. The composition of claim 5 wherein said fragments are derived from a mutant fluorescent protein.
- 7. The composition of claim 6 wherein said complementary fragments differ from the corresponding fragments of the wild-type protein by at least one amino acid.
- 8. The composition of claim 6 wherein said complementary fragments are selected from the group consisting of: Seq. ID NO: 20 to Seq. ID NO: 1067.
- 9. The composition of claim 8 wherein said Seq. ID NO: 20 to Seq. ID NO: 1067 are further fused to a separate molecule.
- 10. A composition comprising complementary fragments of a mutant protein, said fragments generating an optically detectable signal when associated, wherein each fragment is fused to a separate molecule.
- 11. The composition of claim 10 wherein said fragments generate a fluorescent, luminescent or phosphorescent signal when associated.
- 12. The composition of claim 10 wherein said complementary fragments differ from the corresponding fragments of the wild-type protein by at least one amino acid.
- 13. Protein fragment complementation assays for the detection of molecular interactions comprising a reassembly of separate fragments from an optically detectable protein wherein reassembly of the fragments is operated by the interaction of molecular domains fused to each fragment, wherein reassembly of the fragments is independent of other molecular processes and wherein said reassembly is detected by means of reconstitution of activity of said optically detectable protein.
- 14. The assays of claim 13 wherein said fragments generate a fluorescent signal when associated.
- 15. The assays of claim 13 wherein said fragments are derived from a mutant fluorescent protein.
- 16. A method for detecting biomolecular interactions said method comprising:
(a) selecting an appropriate optically detectable protein; (b) effecting fragmentation of said optically detectable protein such that said fragmentation results in reversible loss of protein function; (c) fusing or attaching fragments of said optically detectable protein separately to other molecules; (d) reassociating said protein fragments through interactions of the molecules that are fused or attached to said fragments; and (e) detecting the resulting optical signal.
- 17. The method of claim 16 wherein said optically detectable reporter protein is a mutant fluorescent protein.
Parent Case Info
[0001] This application claims the priority benefit under 35 U.S.C. section 119 of U.S. Provisional Patent Application No. 60/461,133 entitled “Fragments of Fluorescent Proteins for Protein Fragment Complementation Assays”, filed Apr. 9, 2003, which is in its entirety herein incorporated by reference. This Application is also a continuation-in-part of pending U.S. application Ser. No. 10/353,090 filed Jan. 29, 2003; which application is a continuation of pending U.S. application Ser. No. 10/154,758 filed May 24, 2002; which is a continuation of U.S. Ser. No. 09/499,464 filed Feb. 7, 2000; and now U.S. Pat. No. 6,428,951; which is a continuation of U.S. Ser. No. 09/017,412 filed Feb. 2, 1998; and now U.S. Pat. No. 6,270,964.
Provisional Applications (1)
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60461133 |
Apr 2003 |
US |
Continuations (3)
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10154758 |
May 2002 |
US |
Child |
10353090 |
Jan 2003 |
US |
Parent |
09499464 |
Feb 2000 |
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10154758 |
May 2002 |
US |
Parent |
09017412 |
Feb 1998 |
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Child |
09499464 |
Feb 2000 |
US |
Continuation in Parts (1)
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10353090 |
Jan 2003 |
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10724178 |
Dec 2003 |
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