Claims
- 1. A method for enhancing the genetic capability of a mammalian host by employing mammalian cells having exogenous genetic material capable of expression which comprises:
- isolating regenerative mammalian parent cells from a mammlian source compatible with said host;
- combining said regenerative mammalian parent cells with DNA including at least one gene capable of providing a selective advantage over the parent cell due to a naturally occurring deficiency or external stress inducement, said combining being under conditions where said DNA is introduced into said parent cell to produce modified cells; and
- introducing said modified cells into a compatible mammalian host under naturally occurring deficiency conditions or external stress induced conditions in said host providing said selective advantage, whereby said modified cells regenerate in said host at the source of said cells.
- 2. A method according to claim 1, wherein said parent cells are in the dividing phase.
- 3. A method according to claim 1, wherein said one gene providing a selective advantage expresses an enzyme.
- 4. A method according to any of claims 1, 2 or 3, wherein said parent cells are bone marrow stem cells.
- 5. A method according to claim 3, wherein said enzyme is inhibited by a chemotherapeutic drug and said external stress induced conditions in said host comprise administering to said host said chemotherapeutic drug.
- 6. A method according to claim 5, wherein said drug is methotrexate.
- 7. A method according to claim 1, including the additional step of isolating said modified cells from said host.
CROSS-REFERENCE TO RELATED APPLICATIONS
This is a continuation application of pending patent application Ser. No. 134,234, filed Mar. 26, 1980, now U.S. Pat. No. 4,396,601.
Government Interests
The invention described herein was made in the course of, or under, a grant from the United States Public Health Service.
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Number |
Name |
Date |
Kind |
4172124 |
Koprowski et al. |
Oct 1979 |
|
4196265 |
Koprowski et al. |
Apr 1980 |
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4276282 |
Sugimoto et al. |
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Continuations (1)
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Number |
Date |
Country |
Parent |
134234 |
Mar 1980 |
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