Claims
- 1. A method for screening a molecule to determine if it modulates enzymatic activity of an enzyme which hydrolyzes an ADP ribose molecule from an ADP ribose, comprising:(i) transforming or transfecting a cell with an isolated nucleic acid molecule which is at least 80% homologous to the nucleotide sequence set forth at SEQ ID NO: 1; (ii) culturing the transformed or transfected cell of (i) to produce said enzyme; (iii) isolating and purifying said enzyme; (iv) combining said enzyme, said molecule and an ADP ribose polymer; (v) determining degree of hydrolysis in (iv), and (vi) comparing degree of hydrolysis of (v) to degree of hydrolysis obtained by combining said enzyme and said ADP ribose polymer in the absence of said molecule, difference there between indicating said molecule modulates activity of said enzyme.
- 2. The method of claim 1, wherein said enzyme has a molecular weight greater than about 100 kilodaltons as determined by SDS-PAGE.
- 3. The method of claim 1, wherein said enzyme has the amino acid sequence set forth in SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 6.
- 4. The method of claim 1, wherein the amino acid sequence of said enzyme comprises SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO: 14.
- 5. The method of claim 1, wherein said enzyme comprises amino acids 697 to 977 of SEQ ID NO: 4.
- 6. The method of claim 1, wherein the complementary sequence of said nucleic acid molecule hybridizes, under stringent conditions to the nucleotide sequence set forth in SEQ ID NO: 1.
- 7. The method of claim 1, wherein said nucleic acid molecule comprises the nucleotide sequence set forth in SEQ ID NO: 1, 3 or 5.
- 8. A method for screening a molecule to determine if it modulates enzymatic activity of an enzyme which hydrolyzes an ADP-ribose molecule from an ADP ribose polymer, wherein said enzyme comprises the amino acid sequences set forth in SEQ ID NOS: 11, 12, 13 and 14 and has a molecular weight greater than about 100 kilodaltors as determined by SDS-PAGE comprising contacting said molecule, said enzyme and an ADP ribose polymer, determining degree of hydrolysis of said ADP-ribose polymer, and comparing said degree of hydrolysis to the degree of hydrolysis obtained by combining said enzyme and said ADP-ribose polymer in the absence of said molecule, difference there between indicating said molecule modulates activity of said enzyme.
CROSS REFERENCE TO RELATED APPLICATION
This Application claims the benefits of US Provisional Application No. 60/083,768, filed May 1, 1998. The entire disclose of U.S. Provisional Application 60/083,768 is incorporated herein by reference.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
The present invention was supported in part by the National Institutes of Health (Grant CA43894). The United States Government may have certain rights in the invention.
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Provisional Applications (1)
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Number |
Date |
Country |
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60/083768 |
May 1998 |
US |