GENES FOR IMPROVING NUTRIENT UPTAKE AND ABIOTIC STRESS TOLERANCE IN PLANTS

FIELD OF THE DISCLOSURE

The disclosure relates generally to compositions and methods for increasing crop yield.

BACKGROUND

The domestication of many plants has correlated with dramatic increases in yield. Most phenotypic variation occurring in natural populations is continuous and is effected by multiple gene influences. The identification of specific genes responsible for the dramatic differences in yield, in domesticated plants, has become an important focus of agricultural research.

One group of genes affecting yield are the nitrogen utilization efficiency (NUE) genes. These genes have utility for improving the use of nitrogen in crop plants, especially maize. The genes can be used to alter the genetic composition of the plants rendering them more productive with current fertilizer application standards, or maintaining their productive rates with significantly reduced fertilizer input. Increased nitrogen use efficiency can result from enhanced uptake and assimilation of nitrogen fertilizer and/or the subsequent remobilization and reutilization of accumulated nitrogen reserves. Plants containing these genes can therefore be used for the enhancement of yield. Improving the nitrogen use efficiency in corn would increase corn harvestable yield per unit of input nitrogen fertilizer, both in developing nations where access to nitrogen fertilizer is limited and in developed nations were the level of nitrogen use remains high. Nitrogen utilization improvement also allows decreases in on-farm input costs, decreased use and dependence on the non-renewable energy sources required for nitrogen fertilizer production, and decreases the environmental impact of nitrogen fertilizer manufacturing and agricultural use.

Two kinds of genes have been found in plants that regulate plant growth and development. Some genes can enhance plant growth while others suppress plant growth. For example, during leaf development, growth enhancing genes are active to keep young leaves growing. When the leaf reaches full-size, the growth suppressing genes are activated to stop the leaf from further growth.

Insufficient water for optimum growth and development of crop plants is a major obstacle to consistent or increased food production worldwide. Population growth, climate change, irrigation-induced soil salinity, and loss of productive agricultural land to development are among the factors contributing to a need for crop plants which can tolerate drought. Drought stress often results in reduced yield. In maize, this yield loss results in large part from plant failure to set and fill seed in the apical portion of the ear, a phenomenon known as tip kernel abortion.

Plants are restricted to their habitats and must adjust to the prevailing environmental conditions of their surroundings. To cope with abiotic stressors in their habitats, higher plants use a variety of adaptations and plasticity with respect to gene regulation, morphogenesis and metabolism. Adaptation and defense strategies may involve the activation of genes encoding proteins important in the acclimation or defense towards different stressors including drought. Understanding and leveraging the mechanisms of abiotic stress tolerance will have a significant impact on crop productivity.

Methods are needed to enhance drought stress tolerance and to maintain or increase yield under drought conditions.

Crop yield improvements have long been sought and are an age-old problem. Crop yield enhancement has been achieved in the past, by various means, some known, most not. Continued crop yield enhancement will be challenging, demanding specific physiological improvements, such as abiotic stress, and involving more targeted specific approaches, that is, by manipulation of known sets of genes and including both transgenic and breeding approaches. Water limitations globally are the main limitation of crop yield. No prior solution is found to be sufficient to solve the problem of limited crop yield, and thus it remains an unsolved or unfulfilled problem warranting further investigation. This disclosure identifies a set of specific genes that can boost crop yield. It is expected that the main approach for crop yield improvements with these genes is via a judicious ectopic expression, and/or specific native or induced allele selections that could also achieve the yield enhancing effects. Some genes may require reduced expression or expression targeted to specific tissue(s) or developmental profiles.

The present disclosure provides methods to increase crop yield utilizing the disclosed genes controlling plant growth and yield. Plants, plant progeny, seeds and tissues created by these methods are also described.

BRIEF SUMMARY

The disclosure relates generally to compositions and methods for increasing crop yield. Certain embodiments provide methods for enhancing growth of harvestable organs. Certain embodiments provide methods for suppressing growth of non-harvestable organs such as male flower and pollen. Certain embodiments comprise pairs of growth enhancement components and growth suppression components in which the phenotype of the plants is modified to increase harvest index and subsequently crop yield. Certain embodiments provide constructs and methods useful for restructure of plant growth and development through manipulating organ size through cell size or cell numbers.

The present disclosure presents methods to alter the genetic composition of crop plants, especially maize, so that such crops can be more productive with current fertilizer applications and/or as productive with significantly reduced fertilizer input. The utility of this disclosure is then both yield enhancement and reduced fertilizer costs with corresponding reduced impact to the environment. The genetic enhancement of the crop plant's intrinsic genetics in order to enhance nitrogen use efficiency has not been achieved by scientists in the past in any commercially viable sense. This disclosure uniquely uses a highly selected set of maize plants that has been shown to differ in aspects of nitrogen utilization. The plants were then subjected to experiments in mRNA profiling and data analysis to yield a set of genes that are useful for modification of crop plants, especially maize for enhancing nitrogen use efficiency.

Compositions and methods for controlling plant growth for increasing yield in a plant are provided. The compositions include specific gene sequences from sorghum, maize, Arabidopsis thaliana and Pichia angusta. Compositions of the disclosure comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1-5105 as well as variants and fragments thereof.

Polynucleotides encoding the sequences are provided in DNA constructs for expression in a plant of interest. Expression cassettes, plants, plant cells, plant parts and seeds comprising the sequences of the disclosure are further provided. In one aspect, the polynucleotide is operably linked to a constitutive promoter. In another aspect, the polynucleotide is operably linked to a tissue-specific/tissue-preferential promoter.

Methods for modulating the level of a yield improvement sequence in a plant or plant part is provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a yield improvement sequence of the disclosure. The level of yield improvement polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

Methods are provided for increasing abiotic stress in plants. More particularly, the methods of the disclosure find use in agriculture for increasing abiotic stress in dicot and monocot plants. The methods comprise introducing into a plant cell a polynucleotide that encodes a polypeptide operably linked to a promoter that drives expression in a plant.

Methods are further provided for maintaining or increasing yield in plants under drought conditions. Also provided are transformed plants, plant tissues, plant cells and seeds thereof.

DETAILED DESCRIPTION

Methods are provided for increasing stress tolerance, particularly abiotic stress tolerance, in plants. These methods find use, for example, in increasing tolerance to drought stress and maintaining or increasing yield during drought conditions, particularly in agricultural plants.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Unless mentioned otherwise, the techniques employed or contemplated herein are standard methodologies well known to one of ordinary skill in the art. The materials, methods and examples are illustrative only and not limiting. The following is presented by way of illustration and is not intended to limit the scope of the disclosure.

The present disclosures now will be described more fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the disclosure are shown. Indeed, these disclosures may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will satisfy applicable legal requirements. Like numbers refer to like elements throughout.

Many modifications and other embodiments of the disclosures set forth herein will come to mind to one skilled in the art to which these disclosures pertain having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the disclosures are not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims. Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.

The practice of the present disclosure will employ, unless otherwise indicated, conventional techniques of botany, microbiology, tissue culture, molecular biology, chemistry, biochemistry and recombinant DNA technology, which are within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Langenheim and Thimann, BOTANY: PLANT BIOLOGY AND ITS RELATION TO HUMAN AFFAIRS, John Wiley (1982); CELL CULTURE AND SOMATIC CELL GENETICS OF PLANTS, vol. 1, Vasil, ed. (1984); Stanier, et al., THE MICROBIAL WORLD, 5^thed., Prentice-Hall (1986); Dhringra and Sinclair, BASIC PLANT PATHOLOGY METHODS, CRC Press (1985); Maniatis, et al., MOLECULAR CLONING: A LABORATORY MANUAL (1982); DNA CLONING, vols. I and II, Glover, ed. (1985); OLIGONUCLEOTIDE SYNTHESIS, Gait, ed. (1984); NUCLEIC ACID HYBRIDIZATION, Hames and Higgins, eds. (1984); and the series METHODS IN ENZYMOLOGY, Colowick and Kaplan, eds, Academic Press, Inc., San Diego, Calif.

Units, prefixes and symbols may be denoted in their SI accepted form. Unless otherwise indicated, nucleic acids are written left to right in 5′ to 3′ orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively. Numeric ranges are inclusive of the numbers defining the range. Amino acids may be referred to herein by either their commonly known three letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Nucleotides, likewise, may be referred to by their commonly accepted single-letter codes. The terms defined below are more fully defined by reference to the specification as a whole.

In describing the present disclosure, the following terms will be employed and are intended to be defined as indicated below.

By “microbe” is meant any microorganism (including both eukaryotic and prokaryotic microorganisms), such as fungi, yeast, bacteria, actinomycetes, algae and protozoa, as well as other unicellular structures.

By “amplified” is meant the construction of multiple copies of a nucleic acid sequence or multiple copies complementary to the nucleic acid sequence using at least one of the nucleic acid sequences as a template. Amplification systems include the polymerase chain reaction (PCR) system, ligase chain reaction (LCR) system, nucleic acid sequence based amplification (NASBA, Cangene, Mississauga, Ontario), Q-Beta Replicase systems, transcription-based amplification system (TAS), and strand displacement amplification (SDA). See, e.g., DIAGNOSTIC MOLECULAR MICROBIOLOGY: PRINCIPLES AND APPLICATIONS, Persing, et al., eds., American Society for Microbiology, Washington, D.C. (1993). The product of amplification is termed an amplicon.

The term “conservatively modified variants” applies to both amino acid and nucleic acid sequences. With respect to particular nucleic acid sequences, conservatively modified variants refer to those nucleic acids that encode identical or conservatively modified variants of the amino acid sequences. Because of the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For instance, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without altering the encoded polypeptide. Such nucleic acid variations are “silent variations” and represent one species of conservatively modified variation. Every nucleic acid sequence herein that encodes a polypeptide also describes every possible silent variation of the nucleic acid. One of ordinary skill will recognize that each codon in a nucleic acid (except AUG, which is ordinarily the only codon for methionine; one exception is Micrococcus rubens, for which GTG is the methionine codon (Ishizuka, et al., (1993) J. Gen. Microbiol. 139:425-32) can be modified to yield a functionally identical molecule. Accordingly, each silent variation of a nucleic acid, which encodes a polypeptide of the present disclosure, is implicit in each described polypeptide sequence and incorporated herein by reference.

As to amino acid sequences, one of skill will recognize that individual substitutions, deletions or additions to a nucleic acid, peptide, polypeptide or protein sequence which alters, adds or deletes a single amino acid or a small percentage of amino acids in the encoded sequence is a “conservatively modified variant” when the alteration results in the substitution of an amino acid with a chemically similar amino acid. Thus, any number of amino acid residues selected from the group of integers consisting of from 1 to 15 can be so altered. Thus, for example, 1, 2, 3, 4, 5, 7 or 10 alterations can be made. Conservatively modified variants typically provide similar biological activity as the unmodified polypeptide sequence from which they are derived. For example, substrate specificity, enzyme activity, or ligand/receptor binding is generally at least 30%, 40%, 50%, 60%, 70%, 80% or 90%, preferably 60-90% of the native protein for it's native substrate. Conservative substitution tables providing functionally similar amino acids are well known in the art.

The following six groups each contain amino acids that are conservative substitutions for one another:

1) Alanine (A), Serine (S), Threonine (T);

2) Aspartic acid (D), Glutamic acid (E);

3) Asparagine (N), Glutamine (Q);

4) Arginine (R), Lysine (K);

5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); and

6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W).

See also, Creighton, PROTEINS, W.H. Freeman and Co. (1984).

As used herein, “consisting essentially of” means the inclusion of additional sequences to an object polynucleotide where the additional sequences do not selectively hybridize, under stringent hybridization conditions, to the same cDNA as the polynucleotide and where the hybridization conditions include a wash step in 0.1×SSC and 0.1% sodium dodecyl sulfate at 65° C.

By “encoding” or “encoded,” with respect to a specified nucleic acid, is meant comprising the information for translation into the specified protein. A nucleic acid encoding a protein may comprise non-translated sequences (e.g., introns) within translated regions of the nucleic acid, or may lack such intervening non-translated sequences (e.g., as in cDNA). The information by which a protein is encoded is specified by the use of codons. Typically, the amino acid sequence is encoded by the nucleic acid using the “universal” genetic code. However, variants of the universal code, such as is present in some plant, animal and fungal mitochondria, the bacterium Mycoplasma capricolum (Yamao, et al., (1985) Proc. Natl. Acad. Sci. USA 82:2306-9) or the ciliate Macronucleus, may be used when the nucleic acid is expressed using these organisms.

When the nucleic acid is prepared or altered synthetically, advantage can be taken of known codon preferences of the intended host where the nucleic acid is to be expressed. For example, although nucleic acid sequences of the present disclosure may be expressed in both monocotyledonous and dicotyledonous plant species, sequences can be modified to account for the specific codon preferences and GC content preferences of monocotyledonous plants or dicotyledonous plants as these preferences have been shown to differ (Murray, et al., (1989) Nucleic Acids Res. 17:477-98, herein incorporated by reference). Thus, the maize preferred codon for a particular amino acid might be derived from known gene sequences from maize. Maize codon usage for 28 genes from maize plants is listed in Table 4 of Murray, et al., supra.

As used herein, “heterologous” in reference to a nucleic acid is a nucleic acid that originates from a foreign species, or, if from the same species, is substantially modified from its native form in composition and/or genomic locus by deliberate human intervention. For example, a promoter operably linked to a heterologous structural gene is from a species different from that from which the structural gene was derived or, if from the same species, one or both are substantially modified from their original form. A heterologous protein may originate from a foreign species or, if from the same species, is substantially modified from its original form by deliberate human intervention.

By “host cell” is meant a cell, which contains a vector and supports the replication and/or expression of the expression vector. Host cells may be prokaryotic cells such as E. coli, or eukaryotic cells such as yeast, insect, plant, amphibian or mammalian cells. Preferably, host cells are monocotyledonous or dicotyledonous plant cells, including but not limited to maize, sorghum, sunflower, soybean, wheat, alfalfa, rice, cotton, canola, barley, millet and tomato. A particularly preferred monocotyledonous host cell is a maize host cell.

The term “hybridization complex” includes reference to a duplex nucleic acid structure formed by two single-stranded nucleic acid sequences selectively hybridized with each other.

The term “introduced” in the context of inserting a nucleic acid into a cell, means “transfection” or “transformation” or “transduction” and includes reference to the incorporation of a nucleic acid into a eukaryotic or prokaryotic cell where the nucleic acid may be incorporated into the genome of the cell (e.g., chromosome, plasmid, plastid or mitochondrial DNA), converted into an autonomous replicon, or transiently expressed (e.g., transfected mRNA).

The terms “isolated” refers to material, such as a nucleic acid or a protein, which is substantially or essentially free from components which normally accompany or interact with it as found in its naturally occurring environment. The isolated material optionally comprises material not found with the material in its natural environment. Nucleic acids, which are “isolated”, as defined herein, are also referred to as “heterologous” nucleic acids. Unless otherwise stated, the term “yield improvement nucleic acid” means a nucleic acid comprising a polynucleotide (“yield improvement polynucleotide”) encoding a yield improvement polypeptide. The term “Growth Enhancement gene” means a gene that when expressed can increase cell numbers, cell size and dry matter accumulation, resulting in increased organ size, numbers and dry weight. On the opposite, the term “Growth suppression gene” means a gene when expressed can decrease or inhibit cell numbers, cell size and dry matter accumulation, resulting in decreased organ size, numbers and dry weight. The term “yield improvement gene” may include both “Growth Enhancer gene” and “Growth suppressor gene”.

As used herein, “nucleic acid” includes reference to a deoxyribonucleotide or ribonucleotide polymer in either single- or double-stranded form, and unless otherwise limited, encompasses known analogues having the essential nature of natural nucleotides in that they hybridize to single-stranded nucleic acids in a manner similar to naturally occurring nucleotides (e.g., peptide nucleic acids).

By “nucleic acid library” is meant a collection of isolated DNA or RNA molecules, which comprise and substantially represent the entire transcribed fraction of a genome of a specified organism. Construction of exemplary nucleic acid libraries, such as genomic and cDNA libraries, is taught in standard molecular biology references such as Berger and Kimmel, GUIDE TO MOLECULAR CLONING TECHNIQUES, from the series METHODS IN ENZYMOLOGY, vol. 152, Academic Press, Inc., San Diego, Calif. (1987); Sambrook, et al., MOLECULAR CLONING: A LABORATORY MANUAL, 2^nded., vols. 1-3 (1989); and CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Ausubel, et al., eds, Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc. (1994 Supplement).

As used herein “operably linked” includes reference to a functional linkage between a first sequence, such as a promoter and a second sequence, wherein the promoter sequence initiates and mediates transcription of the DNA sequence corresponding to the second sequence. Generally, operably linked means that the nucleic acid sequences being linked are contiguous and, where necessary to join two protein coding regions, contiguous and in the same reading frame.

As used herein, the term “plant” includes reference to whole plants, plant organs (e.g., leaves, stems, roots, etc.), seeds and plant cells and progeny of same. Plant cell, as used herein includes, without limitation, seeds suspension cultures, embryos, meristematic regions, callus tissue, leaves, roots, shoots, gametophytes, sporophytes, pollen and microspores. The class of plants, which can be used in the methods of the disclosure, is generally as broad as the class of higher plants amenable to transformation techniques, including both monocotyledonous and dicotyledonous plants including species from the genera: Cucurbita, Rosa, Vitis, Juglans, Fragaria, Lotus, Medicago, Onobrychis, Trifolium, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Arabidopsis, Brassica, Raphanus, Sinapis, Atropa, Capsicum, Datura, Hyoscyamus, Lycopersicon, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Ciahorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Heterocallis, Nemesis, Pelargonium, Panieum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Browaalia, Glycine, Pisum, Phaseolus, Lolium, Oryza, Avena, Hordeum, Secale, Allium and Triticum. A particularly preferred plant is Zea mays.

As used herein, “yield” includes reference to bushels per acre of a grain crop at harvest, as adjusted for grain moisture (15% typically). Grain moisture is measured in the grain at harvest. The adjusted test weight of grain is determined to be the weight in pounds per bushel, adjusted for grain moisture level at harvest.

As used herein, “polynucleotide” includes reference to a deoxyribopolynucleotide, ribopolynucleotide or analogs thereof that have the essential nature of a natural ribonucleotide in that they hybridize, under stringent hybridization conditions, to substantially the same nucleotide sequence as naturally occurring nucleotides and/or allow translation into the same amino acid(s) as the naturally occurring nucleotide(s). A polynucleotide can be full-length or a subsequence of a native or heterologous structural or regulatory gene. Unless otherwise indicated, the term includes reference to the specified sequence as well as the complementary sequence thereof. Thus, DNAs or RNAs with backbones modified for stability or for other reasons are “polynucleotides” as that term is intended herein. Moreover, DNAs or RNAs comprising unusual bases, such as inosine, or modified bases, such as tritylated bases, to name just two examples, are polynucleotides as the term is used herein. It will be appreciated that a great variety of modifications have been made to DNA and RNA that serve many useful purposes known to those of skill in the art. The term polynucleotide as it is employed herein embraces such chemically, enzymatically or metabolically modified forms of polynucleotides, as well as the chemical forms of DNA and RNA characteristic of viruses and cells, including inter alia, simple and complex cells.

The terms “polypeptide,” “peptide” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical analogue of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers.

As used herein “promoter” includes reference to a region of DNA upstream from the start of transcription and involved in recognition and binding of RNA polymerase and other proteins to initiate transcription. A “plant promoter” is a promoter capable of initiating transcription in plant cells. Exemplary plant promoters include, but are not limited to, those that are obtained from plants, plant viruses and bacteria which comprise genes expressed in plant cells such Agrobacterium or Rhizobium. Examples are promoters that preferentially initiate transcription in certain tissues, such as leaves, roots, seeds, fibres, xylem vessels, tracheids or sclerenchyma. Such promoters are referred to as “tissue preferred.” A “cell type” specific promoter primarily drives expression in certain cell types in one or more organs, for example, vascular cells in roots or leaves. An “inducible” or “regulatable” promoter is a promoter, which is under environmental control. Examples of environmental conditions that may affect transcription by inducible promoters include anaerobic conditions or the presence of light. Another type of promoter is a developmentally regulated promoter, for example, a promoter that drives expression during pollen development. Tissue preferred, cell type specific, developmentally regulated, and inducible promoters constitute the class of “non-constitutive” promoters. A “constitutive” promoter is a promoter, which is active under most environmental conditions.

The term “yield improvement polypeptide” refers to one or more amino acid sequences. The term is also inclusive of fragments, variants, homologs, alleles or precursors (e.g., preproproteins or proproteins) thereof. A “yield improvement protein” comprises a yield improvement polypeptide. Unless otherwise stated, the term “yield improvement nucleic acid” means a nucleic acid comprising a polynucleotide (“yield improvement polynucleotide”) encoding a yield improvement polypeptide.

As used herein “recombinant” includes reference to a cell or vector, that has been modified by the introduction of a heterologous nucleic acid or that the cell is derived from a cell so modified. Thus, for example, recombinant cells express genes that are not found in identical form within the native (non-recombinant) form of the cell or express native genes that are otherwise abnormally expressed, under expressed or not expressed at all as a result of deliberate human intervention. The term “recombinant” as used herein does not encompass the alteration of the cell or vector by naturally occurring events (e.g., spontaneous mutation, natural transformation/transduction/transposition) such as those occurring without deliberate human intervention.

As used herein, a “recombinant expression cassette” is a nucleic acid construct, generated recombinantly or synthetically, with a series of specified nucleic acid elements, which permit transcription of a particular nucleic acid in a target cell. The recombinant expression cassette can be incorporated into a plasmid, chromosome, mitochondrial DNA, plastid DNA, virus or nucleic acid fragment. Typically, the recombinant expression cassette portion of an expression vector includes, among other sequences, a nucleic acid to be transcribed and a promoter.

The terms “residue” or “amino acid residue” or “amino acid” are used interchangeably herein to refer to an amino acid that is incorporated into a protein, polypeptide, or peptide (collectively “protein”). The amino acid may be a naturally occurring amino acid and, unless otherwise limited, may encompass known analogs of natural amino acids that can function in a similar manner as naturally occurring amino acids.

The term “selectively hybridizes” includes reference to hybridization, under stringent hybridization conditions, of a nucleic acid sequence to a specified nucleic acid target sequence to a detectably greater degree (e.g., at least 2-fold over background) than its hybridization to non-target nucleic acid sequences and to the substantial exclusion of non-target nucleic acids. Selectively hybridizing sequences typically have about at least 40% sequence identity, preferably 60-90% sequence identity and most preferably 100% sequence identity (i.e., complementary) with each other.

The terms “stringent conditions” or “stringent hybridization conditions” include reference to conditions under which a probe will hybridize to its target sequence, to a detectably greater degree than other sequences (e.g., at least 2-fold over background).

Stringent conditions are sequence-dependent and will be different in different circumstances. By controlling the stringency of the hybridization and/or washing conditions, target sequences can be identified which can be up to 100% complementary to the probe (homologous probing). Alternatively, stringency conditions can be adjusted to allow some mismatching in sequences so that lower degrees of similarity are detected (heterologous probing). Optimally, the probe is approximately 500 nucleotides in length, but can vary greatly in length from less than 500 nucleotides to equal to the entire length of the target sequence.

Typically, stringent conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short probes (e.g., 10 to 50 nucleotides) and at least about 60° C. for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide or Denhardt's. Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1 M NaCl, 1% SDS (sodium dodecyl sulphate) at 37° C., and a wash in 1× to 2×SSC (20×SSC=3.0 M NaCl/0.3 M trisodium citrate) at 50 to 55° C. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1 M NaCl, 1% SDS at 37° C. and a wash in 0.5× to 1×SSC at 55 to 60° C. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C. and a wash in 0.1×SSC at 60 to 65° C. Specificity is typically the function of post-hybridization washes, the critical factors being the ionic strength and temperature of the final wash solution. For DNA-DNA hybrids, the T_mcan be approximated from the equation of Meinkoth and Wahl, (1984) Anal. Biochem. 138:267-84: T_m=81.5° C.+16.6 (log M)+0.41 (% GC)−0.61 (% form)−500/L; where M is the molarity of monovalent cations, % GC is the percentage of guanosine and cytosine nucleotides in the DNA, % form is the percentage of formamide in the hybridization solution and L is the length of the hybrid in base pairs. The T_mis the temperature (under defined ionic strength and pH) at which 50% of a complementary target sequence hybridizes to a perfectly matched probe. T_mis reduced by about 1° C. for each 1% of mismatching; thus, T_m, hybridization and/or wash conditions can be adjusted to hybridize to sequences of the desired identity. For example, if sequences with ≧90% identity are sought, the T_mcan be decreased 10° C. Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (T_m) for the specific sequence and its complement at a defined ionic strength and pH. However, severely stringent conditions can utilize a hybridization and/or wash at 1, 2, 3 or 4° C. lower than the thermal melting point (T_m); moderately stringent conditions can utilize a hybridization and/or wash at 6, 7, 8, 9 or 10° C. lower than the thermal melting point (T_m); low stringency conditions can utilize a hybridization and/or wash at 11, 12, 13, 14, 15 or 20° C. lower than the thermal melting point (T_m). Using the equation, hybridization and wash compositions, and desired T_m, those of ordinary skill will understand that variations in the stringency of hybridization and/or wash solutions are inherently described. If the desired degree of mismatching results in a T_m, of less than 45° C. (aqueous solution) or 32° C. (formamide solution) it is preferred to increase the SSC concentration so that a higher temperature can be used. An extensive guide to the hybridization of nucleic acids is found in Tijssen, LABORATORY TECHNIQUES IN BIOCHEMISTRY AND MOLECULAR BIOLOGY—HYBRIDIZATION WITH NUCLEIC ACID PROBES, part I, chapter 2, “Overview of principles of hybridization and the strategy of nucleic acid probe assays,” Elsevier, New York (1993) and CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, chapter 2, Ausubel, et al., eds, Greene Publishing and Wiley-Interscience, New York (1995). Unless otherwise stated, in the present application high stringency is defined as hybridization in 4×SSC, 5×Denhardt's (5 g Ficoll, 5 g polyvinypyrrolidone, 5 g bovine serum albumin in 500 ml of water), 0.1 mg/ml boiled salmon sperm DNA, and 25 mM Na phosphate at 65° C., and a wash in 0.1×SSC, 0.1% SDS at 65° C.

As used herein, “transgenic plant” includes reference to a plant, which comprises within its genome a heterologous polynucleotide. Generally, the heterologous polynucleotide is stably integrated within the genome such that the polynucleotide is passed on to successive generations. The heterologous polynucleotide may be integrated into the genome alone or as part of a recombinant expression cassette. “Transgenic” is used herein to include any cell, cell line, callus, tissue, plant part or plant, the genotype of which has been altered by the presence of heterologous nucleic acid including those transgenics initially so altered as well as those created by sexual crosses or asexual propagation from the initial transgenic. The term “transgenic” as used herein does not encompass the alteration of the genome (chromosomal or extra-chromosomal) by conventional plant breeding methods or by naturally occurring events such as random cross-fertilization, non-recombinant viral infection, non-recombinant bacterial transformation, non-recombinant transposition or spontaneous mutation.

As used herein, “vector” includes reference to a nucleic acid used in transfection of a host cell and into which can be inserted a polynucleotide. Vectors are often replicons. Expression vectors permit transcription of a nucleic acid inserted therein.

The following terms are used to describe the sequence relationships between two or more nucleic acids or polynucleotides or polypeptides: (a) “reference sequence,” (b) “comparison window,” (c) “sequence identity,” (d) “percentage of sequence identity” and (e) “substantial identity.”

As used herein, “reference sequence” is a defined sequence used as a basis for sequence comparison. A reference sequence may be a subset or the entirety of a specified sequence; for example, as a segment of a full-length cDNA or gene sequence or the complete cDNA or gene sequence.

As used herein, “comparison window” means includes reference to a contiguous and specified segment of a polynucleotide sequence, wherein the polynucleotide sequence may be compared to a reference sequence and wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. Generally, the comparison window is at least 20 contiguous nucleotides in length, and optionally can be 30, 40, 50, 100 or longer. Those of skill in the art understand that to avoid a high similarity to a reference sequence due to inclusion of gaps in the polynucleotide sequence a gap penalty is typically introduced and is subtracted from the number of matches.

Methods of alignment of nucleotide and amino acid sequences for comparison are well known in the art. The local homology algorithm (BESTFIT) of Smith and Waterman, (1981) Adv. Appl. Math 2:482, may conduct optimal alignment of sequences for comparison; by the homology alignment algorithm (GAP) of Needleman and Wunsch, (1970) J. Mol. Biol. 48:443-53; by the search for similarity method (Tfasta and Fasta) of Pearson and Lipman, (1988) Proc. Natl. Acad. Sci. USA 85:2444; by computerized implementations of these algorithms, including, but not limited to: CLUSTAL in the PC/Gene program by Intelligenetics, Mountain View, Calif., GAP, BESTFIT, BLAST, FASTA, and TFASTA in the Wisconsin Genetics Software Package®, Version 8 (available from Genetics Computer Group (GCG® programs (Accelrys, Inc., San Diego, Calif.)). The CLUSTAL program is well described by Higgins and Sharp, (1988) Gene 73:237-44; Higgins and Sharp, (1989) CABIOS 5:151-3; Corpet, et al., (1988) Nucleic Acids Res. 16:10881-90; Huang, et al., (1992) Computer Applications in the Biosciences 8:155-65 and Pearson, et al., (1994) Meth. Mol. Biol. 24:307-31. The preferred program to use for optimal global alignment of multiple sequences is PileUp (Feng and Doolittle, (1987) J. Mol. Evol., 25:351-60 which is similar to the method described by Higgins and Sharp, (1989) CABIOS 5:151-53 and hereby incorporated by reference). The BLAST family of programs which can be used for database similarity searches includes: BLASTN for nucleotide query sequences against nucleotide database sequences; BLASTX for nucleotide query sequences against protein database sequences; BLASTP for protein query sequences against protein database sequences; TBLASTN for protein query sequences against nucleotide database sequences; and TBLASTX for nucleotide query sequences against nucleotide database sequences. See CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Chapter 19, Ausubel, et al., eds., Greene Publishing and Wiley-Interscience, New York (1995).

GAP uses the algorithm of Needleman and Wunsch, supra, to find the alignment of two complete sequences that maximizes the number of matches and minimizes the number of gaps. GAP considers all possible alignments and gap positions and creates the alignment with the largest number of matched bases and the fewest gaps. It allows for the provision of a gap creation penalty and a gap extension penalty in units of matched bases. GAP must make a profit of gap creation penalty number of matches for each gap it inserts. If a gap extension penalty greater than zero is chosen, GAP must, in addition, make a profit for each gap inserted of the length of the gap times the gap extension penalty. Default gap creation penalty values and gap extension penalty values in Version 10 of the Wisconsin Genetics Software Package® are 8 and 2, respectively. The gap creation and gap extension penalties can be expressed as an integer selected from the group of integers consisting of from 0 to 100. Thus, for example, the gap creation and gap extension penalties can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, 50 or greater.

GAP presents one member of the family of best alignments. There may be many members of this family, but no other member has a better quality. GAP displays four figures of merit for alignments: Quality, Ratio, Identity and Similarity. The Quality is the metric maximized in order to align the sequences. Ratio is the quality divided by the number of bases in the shorter segment. Percent Identity is the percent of the symbols that actually match. Percent Similarity is the percent of the symbols that are similar. Symbols that are across from gaps are ignored. A similarity is scored when the scoring matrix value for a pair of symbols is greater than or equal to 0.50, the similarity threshold. The scoring matrix used in Version 10 of the Wisconsin Genetics Software Package® is BLOSUM62 (see, Henikoff and Henikoff, (1989) Proc. Natl. Acad. Sci. USA 89:10915).

Unless otherwise stated, sequence identity/similarity values provided herein refer to the value obtained using the BLAST 2.0 suite of programs using default parameters (Altschul, et al., (1997) Nucleic Acids Res. 25:3389-402).

As those of ordinary skill in the art will understand, BLAST searches assume that proteins can be modeled as random sequences. However, many real proteins comprise regions of nonrandom sequences, which may be homopolymeric tracts, short-period repeats or regions enriched in one or more amino acids. Such low-complexity regions may be aligned between unrelated proteins even though other regions of the protein are entirely dissimilar. A number of low-complexity filter programs can be employed to reduce such low-complexity alignments. For example, the SEG (Wooten and Federhen, (1993) Comput. Chem. 17:149-63) and XNU (Claverie and States, (1993) Comput. Chem. 17:191-201) low-complexity filters can be employed alone or in combination.

As used herein, “sequence identity” or “identity” in the context of two nucleic acid or polypeptide sequences includes reference to the residues in the two sequences, which are the same when aligned for maximum correspondence over a specified comparison window. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the molecule. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences, which differ by such conservative substitutions, are said to have “sequence similarity” or “similarity.” Means for making this adjustment are well known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., according to the algorithm of Meyers and Miller, (1988) Computer Applic. Biol. Sci. 4:11-17, e.g., as implemented in the program PC/GENE (Intelligenetics, Mountain View, Calif., USA).

As used herein, “percentage of sequence identity” means the value determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison and multiplying the result by 100 to yield the percentage of sequence identity.

The term “substantial identity” of polynucleotide sequences means that a polynucleotide comprises a sequence that has between 50-100% sequence identity, preferably at least 50% sequence identity, preferably at least 60% sequence identity, preferably at least 70%, more preferably at least 80%, more preferably at least 90% and most preferably at least 95%, compared to a reference sequence using one of the alignment programs described using standard parameters. One of skill will recognize that these values can be appropriately adjusted to determine corresponding identity of proteins encoded by two nucleotide sequences by taking into account codon degeneracy, amino acid similarity, reading frame positioning and the like. Substantial identity of amino acid sequences for these purposes normally means sequence identity of between 55-100%, preferably at least 55%, preferably at least 60%, more preferably at least 70%, 80%, 90% and most preferably at least 95%.

Another indication that nucleotide sequences are substantially identical is if two molecules hybridize to each other under stringent conditions. The degeneracy of the genetic code allows for many amino acids substitutions that lead to variety in the nucleotide sequence that code for the same amino acid, hence it is possible that the DNA sequence could code for the same polypeptide but not hybridize to each other under stringent conditions. This may occur, e.g., when a copy of a nucleic acid is created using the maximum codon degeneracy permitted by the genetic code. One indication that two nucleic acid sequences are substantially identical is that the polypeptide, which the first nucleic acid encodes, is immunologically cross reactive with the polypeptide encoded by the second nucleic acid.

The terms “substantial identity” in the context of a peptide indicates that a peptide comprises a sequence with between 55-100% sequence identity to a reference sequence preferably at least 55% sequence identity, preferably 60% preferably 70%, more preferably 80%, most preferably at least 90% or 95% sequence identity to the reference sequence over a specified comparison window. Preferably, optimal alignment is conducted using the homology alignment algorithm of Needleman and Wunsch, supra. An indication that two peptide sequences are substantially identical is that one peptide is immunologically reactive with antibodies raised against the second peptide. Thus, a peptide is substantially identical to a second peptide, for example, where the two peptides differ only by a conservative substitution. In addition, a peptide can be substantially identical to a second peptide when they differ by a non-conservative change if the epitope that the antibody recognizes is substantially identical. Peptides, which are “substantially similar” share sequences as, noted above except that residue positions, which are not identical, may differ by conservative amino acid changes.

The disclosure describes yield improvement polynucleotides and polypeptides. The novel nucleotides and proteins of the disclosure have an expression pattern which indicates that they regulate cell number and thus play an important role in plant development. The polynucleotides are expressed in various plant tissues. The polynucleotides and polypeptides thus provide an opportunity to manipulate plant development to alter seed and vegetative tissue development, timing or composition. This may be used to create a sterile plant, a seedless plant or a plant with altered endosperm composition.

Nucleic Acids

The present disclosure provides, inter alia, isolated nucleic acids of RNA, DNA and analogs and/or chimeras thereof, comprising a yield improvement polynucleotide.

The present disclosure also includes polynucleotides optimized for expression in different organisms. For example, for expression of the polynucleotide in a maize plant, the sequence can be altered to account for specific codon preferences and to alter GC content as according to Murray, et al, supra. Maize codon usage for 28 genes from maize plants is listed in Table 4 of Murray, et al., supra.

The yield improvement nucleic acids of the present disclosure comprise isolated yield improvement polynucleotides which are inclusive of:

- (a) a polynucleotide encoding a yield improvement polypeptide and conservatively modified and polymorphic variants thereof;
- (b) a polynucleotide having at least 70% sequence identity with polynucleotides of (a) or (b);
- (c) complementary sequences of polynucleotides of (a) or (b).

The following table, Table 1, lists the specific identities of the polynucleotides and polypeptides and disclosed herein.

TABLE 1

RR10

Sorghum

Polynucleotide
SEQ ID NO: 1

bicolor

Polypeptide
SEQ ID NO: 2

Genomic
SEQ ID NO: 3405

PTK1

Sorghum

Polynucleotide
SEQ ID NO: 3

bicolor

Polypeptide
SEQ ID NO: 4

Genomic
SEQ ID NO: 3406

ARGOS

Sorghum

Polynucleotide
SEQ ID NO: 5

bicolor

Polypeptide
SEQ ID NO: 6

Genomic
SEQ ID NO: 3407

ARPK

Sorghum

Polynucleotide
SEQ ID NO: 7

bicolor

Polypeptide
SEQ ID NO: 8

Genomic
SEQ ID NO: 3408

BHLH

Sorghum

Polynucleotide
SEQ ID NO: 9

bicolor

Polypeptide
SEQ ID NO: 10

Genomic
SEQ ID NO: 3409

COXVIIa

Sorghum

Polynucleotide
SEQ ID NO: 11

bicolor

Polypeptide
SEQ ID NO: 12

Genomic
SEQ ID NO: 3410

GIRLPK

Sorghum

Polynucleotide
SEQ ID NO: 13

bicolor

Polypeptide
SEQ ID NO: 14

Genomic
SEQ ID NO: 3411

SEU1

Sorghum

Polynucleotide
SEQ ID NO: 15

bicolor

Polypeptide
SEQ ID NO: 16

Genomic
SEQ ID NO: 3412

ERECTA

Sorghum

Polynucleotide
SEQ ID NO: 17

bicolor

Polypeptide
SEQ ID NO: 18

Genomic
SEQ ID NO: 3413

PHDF

Sorghum

Polynucleotide
SEQ ID NO: 19

bicolor

Polypeptide
SEQ ID NO: 20

Genomic
SEQ ID NO: 3414

TFL6

Sorghum

Polynucleotide
SEQ ID NO: 21

bicolor

Polypeptide
SEQ ID NO: 22

Genomic
SEQ ID NO: 3415

TFL8

Sorghum

Polynucleotide
SEQ ID NO: 23

bicolor

Polypeptide
SEQ ID NO: 24

Genomic
SEQ ID NO: 3416

FIE2

Sorghum

Polynucleotide
SEQ ID NO: 25

bicolor

Polypeptide
SEQ ID NO: 26

Genomic
SEQ ID NO: 3417

MEZ2

Sorghum

Polynucleotide
SEQ ID NO: 27

bicolor

Polypeptide
SEQ ID NO: 28

Genomic
SEQ ID NO: 3418

TPS1HA

Sorghum

Polynucleotide
SEQ ID NO: 29

bicolor

Polypeptide
SEQ ID NO: 30

Genomic
SEQ ID NO: 3419

SVP4

Sorghum

Polynucleotide
SEQ ID NO: 31

bicolor

Polypeptide
SEQ ID NO: 32

Genomic
SEQ ID NO: 3420

SILKY1

Sorghum

Polynucleotide
SEQ ID NO: 33

bicolor

Polypeptide
SEQ ID NO: 34

Genomic
SEQ ID NO: 3421

EBNA2

Sorghum

Polynucleotide
SEQ ID NO: 35

bicolor

Polypeptide
SEQ ID NO: 36

Genomic
SEQ ID NO: 3422

MYB

Sorghum

Polynucleotide
SEQ ID NO: 37

bicolor

Polypeptide
SEQ ID NO: 38

Genomic
SEQ ID NO: 3423

ALF1

Sorghum

Polynucleotide
SEQ ID NO: 39

bicolor

Polypeptide
SEQ ID NO: 40

Genomic
SEQ ID NO: 3424

ALF2

Sorghum

Polynucleotide
SEQ ID NO: 41

bicolor

Polypeptide
SEQ ID NO: 42

Genomic
SEQ ID NO: 3425

NDK4

Sorghum

Polynucleotide
SEQ ID NO: 43

bicolor

Polypeptide
SEQ ID NO: 44

Genomic
SEQ ID NO: 3426

PPBP1

Sorghum

Polynucleotide
SEQ ID NO: 45

bicolor

Polypeptide
SEQ ID NO: 46

Genomic
SEQ ID NO: 3427

SPS

Sorghum

Polynucleotide
SEQ ID NO: 47

bicolor

Polypeptide
SEQ ID NO: 48

Genomic
SEQ ID NO: 3428

SIG2B

Sorghum

Polynucleotide
SEQ ID NO: 49

bicolor

Polypeptide
SEQ ID NO: 50

Genomic
SEQ ID NO: 3429

HAP32

Sorghum

Polynucleotide
SEQ ID NO: 51

bicolor

Polypeptide
SEQ ID NO: 52

Genomic
SEQ ID NO: 3430

ARGOS3

Sorghum

Polynucleotide
SEQ ID NO: 53

bicolor

Polypeptide
SEQ ID NO: 54

Genomic
SEQ ID NO: 3431

ARP6

Sorghum

Polynucleotide
SEQ ID NO: 55

bicolor

Polypeptide
SEQ ID NO: 56

Genomic
SEQ ID NO: 3432

HAP3L3

Sorghum

Polynucleotide
SEQ ID NO: 57

bicolor

Polypeptide
SEQ ID NO: 58

Genomic
SEQ ID NO: 3433

CBFA2

Sorghum

Polynucleotide
SEQ ID NO: 59

bicolor

Polypeptide
SEQ ID NO: 60

Genomic
SEQ ID NO: 3434

TFL10

Sorghum

Polynucleotide
SEQ ID NO: 61

bicolor

Polypeptide
SEQ ID NO: 62

Genomic
SEQ ID NO: 3435

TFL13

Sorghum

Polynucleotide
SEQ ID NO: 63

bicolor

Polypeptide
SEQ ID NO: 64

Genomic
SEQ ID NO: 3436

SIG2A

Sorghum

Polynucleotide
SEQ ID NO: 65

bicolor

Polypeptide
SEQ ID NO: 66

Genomic
SEQ ID NO: 3437

ALAAT

Sorghum

Polynucleotide
SEQ ID NO: 67

bicolor

Polypeptide
SEQ ID NO: 68

Genomic
SEQ ID NO: 3438

FBA1

Sorghum

Polynucleotide
SEQ ID NO: 69

bicolor

Polypeptide
SEQ ID NO: 70

Genomic
SEQ ID NO: 3439

SVP3

Sorghum

Polynucleotide
SEQ ID NO: 71

bicolor

Polypeptide
SEQ ID NO: 72

Genomic
SEQ ID NO: 3440

CNR1

Sorghum

Polynucleotide
SEQ ID NO: 73

bicolor

Polypeptide
SEQ ID NO: 74

Genomic
SEQ ID NO: 3441

POL

Sorghum

Polynucleotide
SEQ ID NO: 75

bicolor

Polypeptide
SEQ ID NO: 76

Genomic
SEQ ID NO: 3442

GIP

Sorghum

Polynucleotide
SEQ ID NO: 77

bicolor

Polypeptide
SEQ ID NO: 78

Genomic
SEQ ID NO: 3443

FT6

Sorghum

Polynucleotide
SEQ ID NO: 79

bicolor

Polypeptide
SEQ ID NO: 80

Genomic
SEQ ID NO: 3444

NADHTR

Sorghum

Polynucleotide
SEQ ID NO: 81

bicolor

Polypeptide
SEQ ID NO: 82

Genomic
SEQ ID NO: 3445

RVDH

Sorghum

Polynucleotide
SEQ ID NO: 83

bicolor

Polypeptide
SEQ ID NO: 84

Genomic
SEQ ID NO: 3446

SENC

Sorghum

Polynucleotide
SEQ ID NO: 85

bicolor

Polypeptide
SEQ ID NO: 86

Genomic
SEQ ID NO: 3447

FT4

Sorghum

Polynucleotide
SEQ ID NO: 87

bicolor

Polypeptide
SEQ ID NO: 88

Genomic
SEQ ID NO: 3448

SBP8

Sorghum

Polynucleotide
SEQ ID NO: 89

bicolor

Polypeptide
SEQ ID NO: 90

Genomic
SEQ ID NO: 3449

NRP1

Sorghum

Polynucleotide
SEQ ID NO: 91

bicolor

Polypeptide
SEQ ID NO: 92

Genomic
SEQ ID NO: 3450

TFL16

Sorghum

Polynucleotide
SEQ ID NO: 93

bicolor

Polypeptide
SEQ ID NO: 94

Genomic
SEQ ID NO: 3451

PP2C

Sorghum

Polynucleotide
SEQ ID NO: 95

bicolor

Polypeptide
SEQ ID NO: 96

Genomic
SEQ ID NO: 3452

NUCPU3

Sorghum

Polynucleotide
SEQ ID NO: 97

bicolor

Polypeptide
SEQ ID NO: 98

Genomic
SEQ ID NO: 3453

DTP7

Arabidopsis

Polynucleotide
SEQ ID NO: 99

thaliana

Polypeptide
SEQ ID NO: 100

Genomic
SEQ ID NO: 3454

ARGOS6

Sorghum

Polynucleotide
SEQ ID NO: 101

bicolor

Polypeptide
SEQ ID NO: 102

Genomic
SEQ ID NO: 3455

ARGOS8

Zea mays

Polynucleotide
SEQ ID NO: 103

Polypeptide
SEQ ID NO: 104

Genomic
SEQ ID NO: 3456

ARP7

Sorghum

Polynucleotide
SEQ ID NO: 105

bicolor

Polypeptide
SEQ ID NO: 106

Genomic
SEQ ID NO: 3457

ARGOS9

Sorghum

Polynucleotide
SEQ ID NO: 107

bicolor

Polypeptide
SEQ ID NO: 108

Genomic
SEQ ID NO: 3458

NUCPU7

Sorghum

Polynucleotide
SEQ ID NO: 109

bicolor

Polypeptide
SEQ ID NO: 110

Genomic
SEQ ID NO: 3459

EBP1

Sorghum

Polynucleotide
SEQ ID NO: 111

bicolor

Polypeptide
SEQ ID NO: 112

Genomic
SEQ ID NO: 3460

LRR

Sorghum

Polynucleotide
SEQ ID NO: 113

bicolor

Polypeptide
SEQ ID NO: 114

Genomic
SEQ ID NO: 3461

TFL26

Sorghum

Polynucleotide
SEQ ID NO: 115

bicolor

Polypeptide
SEQ ID NO: 116

Genomic
SEQ ID NO: 3462

SINA

Sorghum

Polynucleotide
SEQ ID NO: 117

bicolor

Polypeptide
SEQ ID NO: 118

Genomic
SEQ ID NO: 3463

ALP

Sorghum

Polynucleotide
SEQ ID NO: 119

bicolor

Polypeptide
SEQ ID NO: 120

Genomic
SEQ ID NO: 3464

GSH1

Sorghum

Polynucleotide
SEQ ID NO: 121

bicolor

Polypeptide
SEQ ID NO: 122

Genomic
SEQ ID NO: 3465

FBA1

Sorghum

Polynucleotide
SEQ ID NO: 123

bicolor

Polypeptide
SEQ ID NO: 124

Genomic
SEQ ID NO: 3466

BZFP1

Sorghum

Polynucleotide
SEQ ID NO: 125

bicolor

Polypeptide
SEQ ID NO: 126

Genomic
SEQ ID NO: 3467

TFL1

Sorghum

Polynucleotide
SEQ ID NO: 127

bicolor

Polypeptide
SEQ ID NO: 128

Genomic
SEQ ID NO: 3468

TFL2

Sorghum

Polynucleotide
SEQ ID NO: 129

bicolor

Polypeptide
SEQ ID NO: 130

Genomic
SEQ ID NO: 3469

TFL3

Sorghum

Polynucleotide
SEQ ID NO: 131

bicolor

Polypeptide
SEQ ID NO: 132

Genomic
SEQ ID NO: 3470

YECPU1

Sorghum

Polynucleotide
SEQ ID NO: 133

bicolor

Polypeptide
SEQ ID NO: 134

Genomic
SEQ ID NO: 3471

DZFP1

Sorghum

Polynucleotide
SEQ ID NO: 135

bicolor

Polypeptide
SEQ ID NO: 136

Genomic
SEQ ID NO: 3472

YECPU2

Sorghum

Polynucleotide
SEQ ID NO: 137

bicolor

Polypeptide
SEQ ID NO: 138

Genomic
SEQ ID NO: 3473

BPIRP1

Sorghum

Polynucleotide
SEQ ID NO: 139

bicolor

Polypeptide
SEQ ID NO: 140

Genomic
SEQ ID NO: 3474

EREFTs

Sorghum

Polynucleotide
SEQ ID NO: 141

bicolor

Polypeptide
SEQ ID NO: 142

Genomic
SEQ ID NO: 3475

YECPU3

Sorghum

Polynucleotide
SEQ ID NO: 143

bicolor

Polypeptide
SEQ ID NO: 144

Genomic
SEQ ID NO: 3476

PC4

Sorghum

Polynucleotide
SEQ ID NO: 145

bicolor

Polypeptide
SEQ ID NO: 146

Genomic
SEQ ID NO: 3477

D9D8

Sorghum

Polynucleotide
SEQ ID NO: 147

bicolor

Polypeptide
SEQ ID NO: 148

Genomic
SEQ ID NO: 3478

ARG4

Sorghum

Polynucleotide
SEQ ID NO: 149

bicolor

Polypeptide
SEQ ID NO: 150

Genomic
SEQ ID NO: 3479

PKL1

Sorghum

Polynucleotide
SEQ ID NO: 151

bicolor

Polypeptide
SEQ ID NO: 152

Genomic
SEQ ID NO: 3480

SERK2

Sorghum

Polynucleotide
SEQ ID NO: 153

bicolor

Polypeptide
SEQ ID NO: 154

Genomic
SEQ ID NO: 3481

WSPL1

Sorghum

Polynucleotide
SEQ ID NO: 155

bicolor

Polypeptide
SEQ ID NO: 156

Genomic
SEQ ID NO: 3482

ZFP1

Sorghum

Polynucleotide
SEQ ID NO: 157

bicolor

Polypeptide
SEQ ID NO: 158

Genomic
SEQ ID NO: 3483

AP2L1

Sorghum

Polynucleotide
SEQ ID NO: 159

bicolor

Polypeptide
SEQ ID NO: 160

Genomic
SEQ ID NO: 3484

HMG

Sorghum

Polynucleotide
SEQ ID NO: 161

bicolor

Polypeptide
SEQ ID NO: 162

Genomic
SEQ ID NO: 3485

CNGC

Sorghum

Polynucleotide
SEQ ID NO: 163

bicolor

Polypeptide
SEQ ID NO: 164

Genomic
SEQ ID NO: 3486

MEIIS5

Sorghum

Polynucleotide
SEQ ID NO: 165

bicolor

Polypeptide
SEQ ID NO: 166

Genomic
SEQ ID NO: 3487

NDBP

Sorghum

Polynucleotide
SEQ ID NO: 167

bicolor

Polypeptide
SEQ ID NO: 168

Genomic
SEQ ID NO: 3488

RGDI

Sorghum

Polynucleotide
SEQ ID NO: 169

bicolor

Polypeptide
SEQ ID NO: 170

Genomic
SEQ ID NO: 3489

SBPPDK

Sorghum

Polynucleotide
SEQ ID NO: 171

bicolor

Polypeptide
SEQ ID NO: 172

Genomic
SEQ ID NO: 3490

SAMPU2

Sorghum

Polynucleotide
SEQ ID NO: 173

bicolor

Polypeptide
SEQ ID NO: 174

Genomic
SEQ ID NO: 3491

CNR06RNAi

Sorghum

Polynucleotide
SEQ ID NO: 175

bicolor

Polypeptide
SEQ ID NO: 176

Genomic
SEQ ID NO: 3492

VRS1RNAi

Sorghum

Polynucleotide
SEQ ID NO: 177

bicolor

Polypeptide
SEQ ID NO: 178

Genomic
SEQ ID NO: 3493

FBL2

Sorghum

Polynucleotide
SEQ ID NO: 179

bicolor

Polypeptide
SEQ ID NO: 180

Genomic
SEQ ID NO: 3494

UCP1

Sorghum

Polynucleotide
SEQ ID NO: 181

bicolor

Polypeptide
SEQ ID NO: 182

Genomic
SEQ ID NO: 3495

CNR02RNAi

Sorghum

Polynucleotide
SEQ ID NO: 183

bicolor

Polypeptide
SEQ ID NO: 184

Genomic
SEQ ID NO: 3496

TTL1RNAi

Sorghum

Polynucleotide
SEQ ID NO: 185

bicolor

Polypeptide
SEQ ID NO: 186

Genomic
SEQ ID NO: 3497

PPDK

Sorghum

Polynucleotide
SEQ ID NO: 187

bicolor

Polypeptide
SEQ ID NO: 188

Genomic
SEQ ID NO: 3498

LEC1LIKERNAi

Sorghum

Polynucleotide
SEQ ID NO: 189

bicolor

Polypeptide
SEQ ID NO: 190

Genomic
SEQ ID NO: 3499

GW21

Sorghum

Polynucleotide
SEQ ID NO: 191

bicolor

Polypeptide
SEQ ID NO: 192

Genomic
SEQ ID NO: 3500

GW22

Sorghum

Polynucleotide
SEQ ID NO: 193

bicolor

Polypeptide
SEQ ID NO: 194

Genomic
SEQ ID NO: 3501

PCYS1

Sorghum

Polynucleotide
SEQ ID NO: 195

bicolor

Polypeptide
SEQ ID NO: 196

Genomic
SEQ ID NO: 3502

SUMOE3

Sorghum

Polynucleotide
SEQ ID NO: 197

bicolor

Polypeptide
SEQ ID NO: 198

Genomic
SEQ ID NO: 3503

M14

Sorghum

Polynucleotide
SEQ ID NO: 199

bicolor

Polypeptide
SEQ ID NO: 200

Genomic
SEQ ID NO: 3504

EDCP647011

Sorghum

Polynucleotide
SEQ ID NO: 201

bicolor

Polypeptide
SEQ ID NO: 202

Genomic
SEQ ID NO: 3505

SPL1

Sorghum

Polynucleotide
SEQ ID NO: 203

bicolor

Polypeptide
SEQ ID NO: 204

Genomic
SEQ ID NO: 3506

ADA2

Sorghum

Polynucleotide
SEQ ID NO: 205

bicolor

Polypeptide
SEQ ID NO: 206

Genomic
SEQ ID NO: 3507

LOBDP1

Sorghum

Polynucleotide
SEQ ID NO: 207

bicolor

Polypeptide
SEQ ID NO: 208

Genomic
SEQ ID NO: 3508

YECP4

Sorghum

Polynucleotide
SEQ ID NO: 209

bicolor

Polypeptide
SEQ ID NO: 210

Genomic
SEQ ID NO: 3509

SAUER2

Sorghum

Polynucleotide
SEQ ID NO: 211

bicolor

Polypeptide
SEQ ID NO: 212

Genomic
SEQ ID NO: 3510

ET3

Sorghum

Polynucleotide
SEQ ID NO: 213

bicolor

Polypeptide
SEQ ID NO: 214

Genomic
SEQ ID NO: 3511

PWWPDPL1

Sorghum

Polynucleotide
SEQ ID NO: 215

bicolor

Polypeptide
SEQ ID NO: 216

Genomic
SEQ ID NO: 3512

GSH1

Sorghum

Polynucleotide
SEQ ID NO: 217

bicolor

Polypeptide
SEQ ID NO: 218

Genomic
SEQ ID NO: 3513

NAC6

Sorghum

Polynucleotide
SEQ ID NO: 219

bicolor

Polypeptide
SEQ ID NO: 220

Genomic
SEQ ID NO: 3514

M8

Sorghum

Polynucleotide
SEQ ID NO: 221

bicolor

Polypeptide
SEQ ID NO: 222

Genomic
SEQ ID NO: 3515

CIPK1

Sorghum

Polynucleotide
SEQ ID NO: 223

bicolor

Polypeptide
SEQ ID NO: 224

Genomic
SEQ ID NO: 3516

TD1

Sorghum

Polynucleotide
SEQ ID NO: 225

bicolor

Polypeptide
SEQ ID NO: 226

Genomic
SEQ ID NO: 3517

ER1

Sorghum

Polynucleotide
SEQ ID NO: 227

bicolor

Polypeptide
SEQ ID NO: 228

Genomic
SEQ ID NO: 3518

YABBY14

Sorghum

Polynucleotide
SEQ ID NO: 229

bicolor

Polypeptide
SEQ ID NO: 230

Genomic
SEQ ID NO: 3519

PCRTC

Sorghum

Polynucleotide
SEQ ID NO: 231

bicolor

Polypeptide
SEQ ID NO: 232

Genomic
SEQ ID NO: 3520

CSZ1

Sorghum

Polynucleotide
SEQ ID NO: 233

bicolor

Polypeptide
SEQ ID NO: 234

Genomic
SEQ ID NO: 3521

ZIMFP

Sorghum

Polynucleotide
SEQ ID NO: 235

bicolor

Polypeptide
SEQ ID NO: 236

Genomic
SEQ ID NO: 3522

WDRP

Sorghum

Polynucleotide
SEQ ID NO: 237

bicolor

Polypeptide
SEQ ID NO: 238

Genomic
SEQ ID NO: 3523

LEA

Sorghum

Polynucleotide
SEQ ID NO: 239

bicolor

Polypeptide
SEQ ID NO: 240

Genomic
SEQ ID NO: 3524

HSP

Sorghum

Polynucleotide
SEQ ID NO: 241

bicolor

Polypeptide
SEQ ID NO: 242

Genomic
SEQ ID NO: 3525

GmSRP

Sorghum

Polynucleotide
SEQ ID NO: 243

bicolor

Polypeptide
SEQ ID NO: 244

Genomic
SEQ ID NO: 3526

LTP

Sorghum

Polynucleotide
SEQ ID NO: 245

bicolor

Polypeptide
SEQ ID NO: 246

Genomic
SEQ ID NO: 3527

IRDR

Sorghum

Polynucleotide
SEQ ID NO: 247

bicolor

Polypeptide
SEQ ID NO: 248

Genomic
SEQ ID NO: 3528

KN1

Sorghum

Polynucleotide
SEQ ID NO: 249

bicolor

Polypeptide
SEQ ID NO: 250

Genomic
SEQ ID NO: 3529

INCW2

Sorghum

Polynucleotide
SEQ ID NO: 251

bicolor

Polypeptide
SEQ ID NO: 252

Genomic
SEQ ID NO: 3530

PPR1

Sorghum

Polynucleotide
SEQ ID NO: 253

bicolor

Polypeptide
SEQ ID NO: 254

Genomic
SEQ ID NO: 3531

Sb01g004490

Sorghum

Polynucleotide
SEQ ID NO: 255

bicolor

Polypeptide
SEQ ID NO: 256

Genomic
SEQ ID NO: 3532

YEP1

Sorghum

Polynucleotide
SEQ ID NO: 257

bicolor

Polypeptide
SEQ ID NO: 258

Genomic
SEQ ID NO: 3533

YEP31

Sorghum

Polynucleotide
SEQ ID NO: 259

bicolor

Polypeptide
SEQ ID NO: 260

Genomic
SEQ ID NO: 3534

LRR3

Sorghum

Polynucleotide
SEQ ID NO: 261

bicolor

Polypeptide
SEQ ID NO: 262

Genomic
SEQ ID NO: 3535

UP

Sorghum

Polynucleotide
SEQ ID NO: 263

bicolor

Polypeptide
SEQ ID NO: 264

Genomic
SEQ ID NO: 3536

GRF5

Sorghum

Polynucleotide
SEQ ID NO: 265

bicolor

Polypeptide
SEQ ID NO: 266

Genomic
SEQ ID NO: 3537

HSD1

Sorghum

Polynucleotide
SEQ ID NO: 267

bicolor

Polypeptide
SEQ ID NO: 268

Genomic
SEQ ID NO: 3538

SDH

Sorghum

Polynucleotide
SEQ ID NO: 269

bicolor

Polypeptide
SEQ ID NO: 270

Genomic
SEQ ID NO: 3539

SUT1

Sorghum

Polynucleotide
SEQ ID NO: 271

bicolor

Polypeptide
SEQ ID NO: 272

Genomic
SEQ ID NO: 3540

SPP1

Sorghum

Polynucleotide
SEQ ID NO: 273

bicolor

Polypeptide
SEQ ID NO: 274

Genomic
SEQ ID NO: 3541

SCL

Sorghum

Polynucleotide
SEQ ID NO: 275

bicolor

Polypeptide
SEQ ID NO: 276

Genomic
SEQ ID NO: 3542

GRP5

Sorghum

Polynucleotide
SEQ ID NO: 277

bicolor

Polypeptide
SEQ ID NO: 278

Genomic
SEQ ID NO: 3543

BA1

Sorghum

Polynucleotide
SEQ ID NO: 279

bicolor

Polypeptide
SEQ ID NO: 280

Genomic
SEQ ID NO: 3544

Bif2

Sorghum

Polynucleotide
SEQ ID NO: 281

bicolor

Polypeptide
SEQ ID NO: 282

Genomic
SEQ ID NO: 3545

Sb03g032340

Sorghum

Polynucleotide
SEQ ID NO: 283

bicolor

Polypeptide
SEQ ID NO: 284

Genomic
SEQ ID NO: 3546

MIPS1

Sorghum

Polynucleotide
SEQ ID NO: 285

bicolor

Polypeptide
SEQ ID NO: 286

Genomic
SEQ ID NO: 3547

TOR

Sorghum

Polynucleotide
SEQ ID NO: 287

bicolor

Polypeptide
SEQ ID NO: 288

Genomic
SEQ ID NO: 3548

Sb07g026630

Sorghum

Polynucleotide
SEQ ID NO: 289

bicolor

Polypeptide
SEQ ID NO: 290

Genomic
SEQ ID NO: 3549

Sb04g029890

Sorghum

Polynucleotide
SEQ ID NO: 291

bicolor

Polypeptide
SEQ ID NO: 292

Genomic
SEQ ID NO: 3550

Sb01g008730

Sorghum

Polynucleotide
SEQ ID NO: 293

bicolor

Polypeptide
SEQ ID NO: 294

Genomic
SEQ ID NO: 3551

Sb01g007580

Sorghum

Polynucleotide
SEQ ID NO: 295

bicolor

Polypeptide
SEQ ID NO: 296

Genomic
SEQ ID NO: 3552

Sb03g011680

Sorghum

Polynucleotide
SEQ ID NO: 297

bicolor

Polypeptide
SEQ ID NO: 298

Genomic
SEQ ID NO: 3553

Sb09g025520

Sorghum

Polynucleotide
SEQ ID NO: 299

bicolor

Polypeptide
SEQ ID NO: 300

Genomic
SEQ ID NO: 3554

Sb07g024970

Sorghum

Polynucleotide
SEQ ID NO: 301

bicolor

Polypeptide
SEQ ID NO: 302

Genomic
SEQ ID NO: 3555

Sb07g025220

Sorghum

Polynucleotide
SEQ ID NO: 303

bicolor

Polypeptide
SEQ ID NO: 304

Genomic
SEQ ID NO: 3556

Sb07g024890

Sorghum

Polynucleotide
SEQ ID NO: 305

bicolor

Polypeptide
SEQ ID NO: 306

Genomic
SEQ ID NO: 3557

Sb05g022280

Sorghum

Polynucleotide
SEQ ID NO: 307

bicolor

Polypeptide
SEQ ID NO: 308

Genomic
SEQ ID NO: 3558

Sb07g026630

Sorghum

Polynucleotide
SEQ ID NO: 309

bicolor

Polypeptide
SEQ ID NO: 310

Genomic
SEQ ID NO: 3559

Sb04g031170

Sorghum

Polynucleotide
SEQ ID NO: 311

bicolor

Polypeptide
SEQ ID NO: 312

Genomic
SEQ ID NO: 3560

Sb01g023750

Sorghum

Polynucleotide
SEQ ID NO: 313

bicolor

Polypeptide
SEQ ID NO: 314

Genomic
SEQ ID NO: 3561

Sb10g006910

Sorghum

Polynucleotide
SEQ ID NO: 315

bicolor

Polypeptide
SEQ ID NO: 316

Genomic
SEQ ID NO: 3562

Sb06g033870

Sorghum

Polynucleotide
SEQ ID NO: 317

bicolor

Polypeptide
SEQ ID NO: 318

Genomic
SEQ ID NO: 3563

Sb03g034260

Sorghum

Polynucleotide
SEQ ID NO: 319

bicolor

Polypeptide
SEQ ID NO: 320

Genomic
SEQ ID NO: 3564

Sb06g033840

Sorghum

Polynucleotide
SEQ ID NO: 321

bicolor

Polypeptide
SEQ ID NO: 322

Genomic
SEQ ID NO: 3565

Sb04g006250

Sorghum

Polynucleotide
SEQ ID NO: 323

bicolor

Polypeptide
SEQ ID NO: 324

Genomic
SEQ ID NO: 3566

Sb06g033970

Sorghum

Polynucleotide
SEQ ID NO: 325

bicolor

Polypeptide
SEQ ID NO: 326

Genomic
SEQ ID NO: 3567

Sb01g023740

Sorghum

Polynucleotide
SEQ ID NO: 327

bicolor

Polypeptide
SEQ ID NO: 328

Genomic
SEQ ID NO: 3568

Sb10g029510

Sorghum

Polynucleotide
SEQ ID NO: 329

bicolor

Polypeptide
SEQ ID NO: 330

Genomic
SEQ ID NO: 3569

Sb04g003690

Sorghum

Polynucleotide
SEQ ID NO: 331

bicolor

Polypeptide
SEQ ID NO: 332

Genomic
SEQ ID NO: 3570

Sb10g027830

Sorghum

Polynucleotide
SEQ ID NO: 333

bicolor

Polypeptide
SEQ ID NO: 334

Genomic
SEQ ID NO: 3571

Sb10g027790

Sorghum

Polynucleotide
SEQ ID NO: 335

bicolor

Polypeptide
SEQ ID NO: 336

Genomic
SEQ ID NO: 3572

Sb04g036060

Sorghum

Polynucleotide
SEQ ID NO: 337

bicolor

Polypeptide
SEQ ID NO: 338

Genomic
SEQ ID NO: 3573

Sb06g001970

Sorghum

Polynucleotide
SEQ ID NO: 339

bicolor

Polypeptide
SEQ ID NO: 340

Genomic
SEQ ID NO: 3574

Sb01g011700

Sorghum

Polynucleotide
SEQ ID NO: 341

bicolor

Polypeptide
SEQ ID NO: 342

Genomic
SEQ ID NO: 3575

Sb01g006960

Sorghum

Polynucleotide
SEQ ID NO: 343

bicolor

Polypeptide
SEQ ID NO: 344

Genomic
SEQ ID NO: 3576

Sb03g041740

Sorghum

Polynucleotide
SEQ ID NO: 345

bicolor

Polypeptide
SEQ ID NO: 346

Genomic
SEQ ID NO: 3577

Sb01g011780

Sorghum

Polynucleotide
SEQ ID NO: 347

bicolor

Polypeptide
SEQ ID NO: 348

Genomic
SEQ ID NO: 3578

Sb06g012520

Sorghum

Polynucleotide
SEQ ID NO: 349

bicolor

Polypeptide
SEQ ID NO: 350

Genomic
SEQ ID NO: 3579

Sb09g029240

Sorghum

Polynucleotide
SEQ ID NO: 351

bicolor

Polypeptide
SEQ ID NO: 352

Genomic
SEQ ID NO: 3580

Sb09g002810

Sorghum

Polynucleotide
SEQ ID NO: 353

bicolor

Polypeptide
SEQ ID NO: 354

Genomic
SEQ ID NO: 3581

Sb01g049650

Sorghum

Polynucleotide
SEQ ID NO: 355

bicolor

Polypeptide
SEQ ID NO: 356

Genomic
SEQ ID NO: 3582

Sb01g032250

Sorghum

Polynucleotide
SEQ ID NO: 357

bicolor

Polypeptide
SEQ ID NO: 358

Genomic
SEQ ID NO: 3583

Sb07g003690

Sorghum

Polynucleotide
SEQ ID NO: 359

bicolor

Polypeptide
SEQ ID NO: 360

Genomic
SEQ ID NO: 3584

Sb04g035410

Sorghum

Polynucleotide
SEQ ID NO: 361

bicolor

Polypeptide
SEQ ID NO: 362

Genomic
SEQ ID NO: 3585

Sb01g030930

Sorghum

Polynucleotide
SEQ ID NO: 363

bicolor

Polypeptide
SEQ ID NO: 364

Genomic
SEQ ID NO: 3586

Sb03g042440

Sorghum

Polynucleotide
SEQ ID NO: 365

bicolor

Polypeptide
SEQ ID NO: 366

Genomic
SEQ ID NO: 3587

Sb10g002070

Sorghum

Polynucleotide
SEQ ID NO: 367

bicolor

Polypeptide
SEQ ID NO: 368

Genomic
SEQ ID NO: 3588

Sb09g029110

Sorghum

Polynucleotide
SEQ ID NO: 369

bicolor

Polypeptide
SEQ ID NO: 370

Genomic
SEQ ID NO: 3589

Sb05g004100

Sorghum

Polynucleotide
SEQ ID NO: 371

bicolor

Polypeptide
SEQ ID NO: 372

Genomic
SEQ ID NO: 3590

Sb01g006100

Sorghum

Polynucleotide
SEQ ID NO: 373

bicolor

Polypeptide
SEQ ID NO: 374

Genomic
SEQ ID NO: 3591

NIR1

Sorghum

Polynucleotide
SEQ ID NO: 375

bicolor

Polypeptide
SEQ ID NO: 376

Genomic
SEQ ID NO: 3592

GLN1

Sorghum

Polynucleotide
SEQ ID NO: 377

bicolor

Polypeptide
SEQ ID NO: 378

Genomic
SEQ ID NO: 3593

NR1

Sorghum

Polynucleotide
SEQ ID NO: 379

bicolor

Polypeptide
SEQ ID NO: 380

Genomic
SEQ ID NO: 3594

Sb10g026570

Sorghum

Polynucleotide
SEQ ID NO: 381

bicolor

Polypeptide
SEQ ID NO: 382

Genomic
SEQ ID NO: 3595

Sb01g028950

Sorghum

Polynucleotide
SEQ ID NO: 383

bicolor

Polypeptide
SEQ ID NO: 384

Genomic
SEQ ID NO: 3596

NLM6

Sorghum

Polynucleotide
SEQ ID NO: 385

bicolor

Polypeptide
SEQ ID NO: 386

Genomic
SEQ ID NO: 3597

NRT1

Sorghum

Polynucleotide
SEQ ID NO: 387

bicolor

Polypeptide
SEQ ID NO: 388

Genomic
SEQ ID NO: 3598

NAC100

Sorghum

Polynucleotide
SEQ ID NO: 389

bicolor

Polypeptide
SEQ ID NO: 390

Genomic
SEQ ID NO: 3599

PIP1E

Sorghum

Polynucleotide
SEQ ID NO: 391

bicolor

Polypeptide
SEQ ID NO: 392

Genomic
SEQ ID NO: 3600

Sb05g004100

Sorghum

Polynucleotide
SEQ ID NO: 393

bicolor

Polypeptide
SEQ ID NO: 394

Genomic
SEQ ID NO: 3601

AMP1

Sorghum

Polynucleotide
SEQ ID NO: 395

bicolor

Polypeptide
SEQ ID NO: 396

Genomic
SEQ ID NO: 3602

Sb03g036560

Sorghum

Polynucleotide
SEQ ID NO: 397

bicolor

Polypeptide
SEQ ID NO: 398

Genomic
SEQ ID NO: 3603

MPK3

Sorghum

Polynucleotide
SEQ ID NO: 399

bicolor

Polypeptide
SEQ ID NO: 400

Genomic
SEQ ID NO: 3604

ERD9

Sorghum

Polynucleotide
SEQ ID NO: 401

bicolor

Polypeptide
SEQ ID NO: 402

Genomic
SEQ ID NO: 3605

Sb09g013790

Sorghum

Polynucleotide
SEQ ID NO: 403

bicolor

Polypeptide
SEQ ID NO: 404

Genomic
SEQ ID NO: 3606

SEL1

Sorghum

Polynucleotide
SEQ ID NO: 405

bicolor

Polypeptide
SEQ ID NO: 406

Genomic
SEQ ID NO: 3607

AKHSDH

Sorghum

Polynucleotide
SEQ ID NO: 407

bicolor

Polypeptide
SEQ ID NO: 408

Genomic
SEQ ID NO: 3608

Sb09g001560

Sorghum

Polynucleotide
SEQ ID NO: 409

bicolor

Polypeptide
SEQ ID NO: 410

Genomic
SEQ ID NO: 3609

MAT2

Sorghum

Polynucleotide
SEQ ID NO: 411

bicolor

Polypeptide
SEQ ID NO: 412

Genomic
SEQ ID NO: 3610

GLN1

Sorghum

Polynucleotide
SEQ ID NO: 413

bicolor

Polypeptide
SEQ ID NO: 414

Genomic
SEQ ID NO: 3611

Sb03g028760

Sorghum

Polynucleotide
SEQ ID NO: 415

bicolor

Polypeptide
SEQ ID NO: 416

Genomic
SEQ ID NO: 3612

Sb03g040180

Sorghum

Polynucleotide
SEQ ID NO: 417

bicolor

Polypeptide
SEQ ID NO: 418

Genomic
SEQ ID NO: 3613

Sb09g006480

Sorghum

Polynucleotide
SEQ ID NO: 419

bicolor

Polypeptide
SEQ ID NO: 420

Genomic
SEQ ID NO: 3614

Sb08g003730

Sorghum

Polynucleotide
SEQ ID NO: 421

bicolor

Polypeptide
SEQ ID NO: 422

Genomic
SEQ ID NO: 3615

Sb03g031310

Sorghum

Polynucleotide
SEQ ID NO: 423

bicolor

Polypeptide
SEQ ID NO: 424

Genomic
SEQ ID NO: 3616

Sb03g041220

Sorghum

Polynucleotide
SEQ ID NO: 425

bicolor

Polypeptide
SEQ ID NO: 426

Genomic
SEQ ID NO: 3617

Sb01g044110

Sorghum

Polynucleotide
SEQ ID NO: 427

bicolor

Polypeptide
SEQ ID NO: 428

Genomic
SEQ ID NO: 3618

Sb01g003680

Sorghum

Polynucleotide
SEQ ID NO: 429

bicolor

Polypeptide
SEQ ID NO: 430

Genomic
SEQ ID NO: 3619

Sb01g042740

Sorghum

Polynucleotide
SEQ ID NO: 431

bicolor

Polypeptide
SEQ ID NO: 432

Genomic
SEQ ID NO: 3620

Sb09g002840

Sorghum

Polynucleotide
SEQ ID NO: 433

bicolor

Polypeptide
SEQ ID NO: 434

Genomic
SEQ ID NO: 3621

Sb01g003710

Sorghum

Polynucleotide
SEQ ID NO: 435

bicolor

Polypeptide
SEQ ID NO: 436

Genomic
SEQ ID NO: 3622

Sb10g009590

Sorghum

Polynucleotide
SEQ ID NO: 437

bicolor

Polypeptide
SEQ ID NO: 438

Genomic
SEQ ID NO: 3623

Sb10g029870

Sorghum

Polynucleotide
SEQ ID NO: 439

bicolor

Polypeptide
SEQ ID NO: 440

Genomic
SEQ ID NO: 3624

Sb09g003830

Sorghum

Polynucleotide
SEQ ID NO: 441

bicolor

Polypeptide
SEQ ID NO: 442

Genomic
SEQ ID NO: 3625

Sb01g042450

Sorghum

Polynucleotide
SEQ ID NO: 443

bicolor

Polypeptide
SEQ ID NO: 444

Genomic
SEQ ID NO: 3626

Sb02g037580

Sorghum

Polynucleotide
SEQ ID NO: 445

bicolor

Polypeptide
SEQ ID NO: 446

Genomic
SEQ ID NO: 3627

Sb03g031780

Sorghum

Polynucleotide
SEQ ID NO: 447

bicolor

Polypeptide
SEQ ID NO: 448

Genomic
SEQ ID NO: 3628

Sb02g023230

Sorghum

Polynucleotide
SEQ ID NO: 449

bicolor

Polypeptide
SEQ ID NO: 450

Genomic
SEQ ID NO: 3629

Sb02g001600

Sorghum

Polynucleotide
SEQ ID NO: 451

bicolor

Polypeptide
SEQ ID NO: 452

Genomic
SEQ ID NO: 3630

Sb08g017630

Sorghum

Polynucleotide
SEQ ID NO: 453

bicolor

Polypeptide
SEQ ID NO: 454

Genomic
SEQ ID NO: 3631

Sb04g037800

Sorghum

Polynucleotide
SEQ ID NO: 455

bicolor

Polypeptide
SEQ ID NO: 456

Genomic
SEQ ID NO: 3632

Sb02g010830

Sorghum

Polynucleotide
SEQ ID NO: 457

bicolor

Polypeptide
SEQ ID NO: 458

Genomic
SEQ ID NO: 3633

Sb09g022710

Sorghum

Polynucleotide
SEQ ID NO: 459

bicolor

Polypeptide
SEQ ID NO: 460

Genomic
SEQ ID NO: 3634

Sb07g005200

Sorghum

Polynucleotide
SEQ ID NO: 461

bicolor

Polypeptide
SEQ ID NO: 462

Genomic
SEQ ID NO: 3635

Sb01g017230

Sorghum

Polynucleotide
SEQ ID NO: 463

bicolor

Polypeptide
SEQ ID NO: 464

Genomic
SEQ ID NO: 3636

Sb01g047140

Sorghum

Polynucleotide
SEQ ID NO: 465

bicolor

Polypeptide
SEQ ID NO: 466

Genomic
SEQ ID NO: 3637

Sb02g010760

Sorghum

Polynucleotide
SEQ ID NO: 467

bicolor

Polypeptide
SEQ ID NO: 468

Genomic
SEQ ID NO: 3638

Sb01g045720

Sorghum

Polynucleotide
SEQ ID NO: 469

bicolor

Polypeptide
SEQ ID NO: 470

Genomic
SEQ ID NO: 3639

Sb04g030600

Sorghum

Polynucleotide
SEQ ID NO: 471

bicolor

Polypeptide
SEQ ID NO: 472

Genomic
SEQ ID NO: 3640

Sb03g003100

Sorghum

Polynucleotide
SEQ ID NO: 473

bicolor

Polypeptide
SEQ ID NO: 474

Genomic
SEQ ID NO: 3641

Sb08g015550

Sorghum

Polynucleotide
SEQ ID NO: 475

bicolor

Polypeptide
SEQ ID NO: 476

Genomic
SEQ ID NO: 3642

Sb06g033310

Sorghum

Polynucleotide
SEQ ID NO: 477

bicolor

Polypeptide
SEQ ID NO: 478

Genomic
SEQ ID NO: 3643

Sb03g011700

Sorghum

Polynucleotide
SEQ ID NO: 479

bicolor

Polypeptide
SEQ ID NO: 480

Genomic
SEQ ID NO: 3644

Sb04g032900

Sorghum

Polynucleotide
SEQ ID NO: 481

bicolor

Polypeptide
SEQ ID NO: 482

Genomic
SEQ ID NO: 3645

Sb02g010830

Sorghum

Polynucleotide
SEQ ID NO: 483

bicolor

Polypeptide
SEQ ID NO: 484

Genomic
SEQ ID NO: 3646

Sb09g019740

Sorghum

Polynucleotide
SEQ ID NO: 485

bicolor

Polypeptide
SEQ ID NO: 486

Genomic
SEQ ID NO: 3647

Sb06g033600

Sorghum

Polynucleotide
SEQ ID NO: 487

bicolor

Polypeptide
SEQ ID NO: 488

Genomic
SEQ ID NO: 3648

Sb04g032430

Sorghum

Polynucleotide
SEQ ID NO: 489

bicolor

Polypeptide
SEQ ID NO: 490

Genomic
SEQ ID NO: 3649

Sb01g041700

Sorghum

Polynucleotide
SEQ ID NO: 491

bicolor

Polypeptide
SEQ ID NO: 492

Genomic
SEQ ID NO: 3650

Sb04g026650

Sorghum

Polynucleotide
SEQ ID NO: 493

bicolor

Polypeptide
SEQ ID NO: 494

Genomic
SEQ ID NO: 3651

Sb04g024150

Sorghum

Polynucleotide
SEQ ID NO: 495

bicolor

Polypeptide
SEQ ID NO: 496

Genomic
SEQ ID NO: 3652

Sb04g032900

Sorghum

Polynucleotide
SEQ ID NO: 497

bicolor

Polypeptide
SEQ ID NO: 498

Genomic
SEQ ID NO: 3653

Sb03g003200

Sorghum

Polynucleotide
SEQ ID NO: 499

bicolor

Polypeptide
SEQ ID NO: 500

Genomic
SEQ ID NO: 3654

Sb03g006420

Sorghum

Polynucleotide
SEQ ID NO: 501

bicolor

Polypeptide
SEQ ID NO: 502

Genomic
SEQ ID NO: 3655

Sb01g002960

Sorghum

Polynucleotide
SEQ ID NO: 503

bicolor

Polypeptide
SEQ ID NO: 504

Genomic
SEQ ID NO: 3656

Sb02g000780

Sorghum

Polynucleotide
SEQ ID NO: 505

bicolor

Polypeptide
SEQ ID NO: 506

Genomic
SEQ ID NO: 3657

Sb10g009590

Sorghum

Polynucleotide
SEQ ID NO: 507

bicolor

Polypeptide
SEQ ID NO: 508

Genomic
SEQ ID NO: 3658

Sb05g019500

Sorghum

Polynucleotide
SEQ ID NO: 509

bicolor

Polypeptide
SEQ ID NO: 510

Genomic
SEQ ID NO: 3659

Sb08g007586

Sorghum

Polynucleotide
SEQ ID NO: 511

bicolor

Polypeptide
SEQ ID NO: 512

Genomic
SEQ ID NO: 3660

Sb01g018430

Sorghum

Polynucleotide
SEQ ID NO: 513

bicolor

Polypeptide
SEQ ID NO: 514

Genomic
SEQ ID NO: 3661

Sb03g034260

Sorghum

Polynucleotide
SEQ ID NO: 515

bicolor

Polypeptide
SEQ ID NO: 516

Genomic
SEQ ID NO: 3662

Sb03g027360

Sorghum

Polynucleotide
SEQ ID NO: 517

bicolor

Polypeptide
SEQ ID NO: 518

Genomic
SEQ ID NO: 3663

Sb10g027790

Sorghum

Polynucleotide
SEQ ID NO: 519

bicolor

Polypeptide
SEQ ID NO: 520

Genomic
SEQ ID NO: 3664

Sb10g002890

Sorghum

Polynucleotide
SEQ ID NO: 521

bicolor

Polypeptide
SEQ ID NO: 522

Genomic
SEQ ID NO: 3665

Sb06g024150

Sorghum

Polynucleotide
SEQ ID NO: 523

bicolor

Polypeptide
SEQ ID NO: 524

Genomic
SEQ ID NO: 3666

Sb06g024150

Sorghum

Polynucleotide
SEQ ID NO: 525

bicolor

Polypeptide
SEQ ID NO: 526

Genomic
SEQ ID NO: 3667

Sb10g027790

Sorghum

Polynucleotide
SEQ ID NO: 527

bicolor

Polypeptide
SEQ ID NO: 528

Genomic
SEQ ID NO: 3668

Sb04g028020

Sorghum

Polynucleotide
SEQ ID NO: 529

bicolor

Polypeptide
SEQ ID NO: 530

Genomic
SEQ ID NO: 3669

Sb10g008090

Sorghum

Polynucleotide
SEQ ID NO: 531

bicolor

Polypeptide
SEQ ID NO: 532

Genomic
SEQ ID NO: 3670

RHS1

Sorghum

Polynucleotide
SEQ ID NO: 533

bicolor

Polypeptide
SEQ ID NO: 534

Genomic
SEQ ID NO: 3671

RHS2

Sorghum

Polynucleotide
SEQ ID NO: 535

bicolor

Polypeptide
SEQ ID NO: 536

Genomic
SEQ ID NO: 3672

RHS3

Sorghum

Polynucleotide
SEQ ID NO: 537

bicolor

Polypeptide
SEQ ID NO: 538

Genomic
SEQ ID NO: 3673

RHS4

Arabidopsis

Polynucleotide
SEQ ID NO: 539

thaliana

Polypeptide
SEQ ID NO: 540

Genomic
SEQ ID NO: 3674

RHS5

Sorghum

Polynucleotide
SEQ ID NO: 541

bicolor

Polypeptide
SEQ ID NO: 542

Genomic
SEQ ID NO: 3675

RHS6

Sorghum

Polynucleotide
SEQ ID NO: 543

bicolor

Polypeptide
SEQ ID NO: 544

Genomic
SEQ ID NO: 3676

RHS7

Sorghum

Polynucleotide
SEQ ID NO: 545

bicolor

Polypeptide
SEQ ID NO: 546

Genomic
SEQ ID NO: 3677

RHS8

Sorghum

Polynucleotide
SEQ ID NO: 547

bicolor

Polypeptide
SEQ ID NO: 548

Genomic
SEQ ID NO: 3678

RHS9

Sorghum

Polynucleotide
SEQ ID NO: 549

bicolor

Polypeptide
SEQ ID NO: 550

Genomic
SEQ ID NO: 3679

Sb03g029150

Sorghum

Polynucleotide
SEQ ID NO: 551

bicolor

Polypeptide
SEQ ID NO: 552

Genomic
SEQ ID NO: 3680

RHS10

Sorghum

Polynucleotide
SEQ ID NO: 553

bicolor

Polypeptide
SEQ ID NO: 554

Genomic
SEQ ID NO: 3681

Sb01g015140

Sorghum

Polynucleotide
SEQ ID NO: 555

bicolor

Polypeptide
SEQ ID NO: 556

Genomic
SEQ ID NO: 3682

RHS11

Sorghum

Polynucleotide
SEQ ID NO: 557

bicolor

Polypeptide
SEQ ID NO: 558

Genomic
SEQ ID NO: 3683

RHS12

Sorghum

Polynucleotide
SEQ ID NO: 559

bicolor

Polypeptide
SEQ ID NO: 560

Genomic
SEQ ID NO: 3684

Sb03g006140

Sorghum

Polynucleotide
SEQ ID NO: 561

bicolor

Polypeptide
SEQ ID NO: 562

Genomic
SEQ ID NO: 3685

Sb07g019540

Sorghum

Polynucleotide
SEQ ID NO: 563

bicolor

Polypeptide
SEQ ID NO: 564

Genomic
SEQ ID NO: 3686

RHS13

Arabidopsis

Polynucleotide
SEQ ID NO: 565

thaliana

Polypeptide
SEQ ID NO: 566

Genomic
SEQ ID NO: 3687

At4g15740

Arabidopsis

Polynucleotide
SEQ ID NO: 567

thaliana

Polypeptide
SEQ ID NO: 568

Genomic
SEQ ID NO: 3688

RHS14

Sorghum

Polynucleotide
SEQ ID NO: 569

bicolor

Polypeptide
SEQ ID NO: 570

Genomic
SEQ ID NO: 3689

RHS15

Sorghum

Polynucleotide
SEQ ID NO: 571

bicolor

Polypeptide
SEQ ID NO: 572

Genomic
SEQ ID NO: 3690

RHS16

Sorghum

Polynucleotide
SEQ ID NO: 573

bicolor

Polypeptide
SEQ ID NO: 574

Genomic
SEQ ID NO: 3691

Sb07g023200

Sorghum

Polynucleotide
SEQ ID NO: 575

bicolor

Polypeptide
SEQ ID NO: 576

Genomic
SEQ ID NO: 3692

Sb02g026818

Sorghum

Polynucleotide
SEQ ID NO: 577

bicolor

Polypeptide
SEQ ID NO: 578

Genomic
SEQ ID NO: 3693

RHS17

Sorghum

Polynucleotide
SEQ ID NO: 579

bicolor

Polypeptide
SEQ ID NO: 580

Genomic
SEQ ID NO: 3694

Sb04g010270

Sorghum

Polynucleotide
SEQ ID NO: 581

bicolor

Polypeptide
SEQ ID NO: 582

Genomic
SEQ ID NO: 3695

RHS18

Sorghum

Polynucleotide
SEQ ID NO: 583

bicolor

Polypeptide
SEQ ID NO: 584

Genomic
SEQ ID NO: 3696

Sb01g039360

Sorghum

Polynucleotide
SEQ ID NO: 585

bicolor

Polypeptide
SEQ ID NO: 586

Genomic
SEQ ID NO: 3697

RHS19

Sorghum

Polynucleotide
SEQ ID NO: 587

bicolor

Polypeptide
SEQ ID NO: 588

Genomic
SEQ ID NO: 3698

Sb04g003090

Sorghum

Polynucleotide
SEQ ID NO: 589

bicolor

Polypeptide
SEQ ID NO: 590

Genomic
SEQ ID NO: 3699

Sb01g030590

Sorghum

Polynucleotide
SEQ ID NO: 591

bicolor

Polypeptide
SEQ ID NO: 592

Genomic
SEQ ID NO: 3700

Sb04g003090

Sorghum

Polynucleotide
SEQ ID NO: 593

bicolor

Polypeptide
SEQ ID NO: 594

Genomic
SEQ ID NO: 3701

Sb01g030590

Sorghum

Polynucleotide
SEQ ID NO: 595

bicolor

Polypeptide
SEQ ID NO: 596

Genomic
SEQ ID NO: 3702

Sb02g034435

Sorghum

Polynucleotide
SEQ ID NO: 597

bicolor

Polypeptide
SEQ ID NO: 598

Genomic
SEQ ID NO: 3703

At1g58270

Arabidopsis

Polynucleotide
SEQ ID NO: 599

thaliana

Polypeptide
SEQ ID NO: 600

Genomic
SEQ ID NO: 3704

Sb04g026290

Sorghum

Polynucleotide
SEQ ID NO: 601

bicolor

Polypeptide
SEQ ID NO: 602

Genomic
SEQ ID NO: 3705

Sb04g030020

Sorghum

Polynucleotide
SEQ ID NO: 603

bicolor

Polypeptide
SEQ ID NO: 604

Genomic
SEQ ID NO: 3706

Sb03g043660

Sorghum

Polynucleotide
SEQ ID NO: 605

bicolor

Polypeptide
SEQ ID NO: 606

Genomic
SEQ ID NO: 3707

At5g02330

Arabidopsis

Polynucleotide
SEQ ID NO: 607

thaliana

Polypeptide
SEQ ID NO: 608

Genomic
SEQ ID NO: 3708

Sb02g020860

Sorghum

Polynucleotide
SEQ ID NO: 609

bicolor

Polypeptide
SEQ ID NO: 610

Genomic
SEQ ID NO: 3709

Sb04g000750

Sorghum

Polynucleotide
SEQ ID NO: 611

bicolor

Polypeptide
SEQ ID NO: 612

Genomic
SEQ ID NO: 3710

Sb02g005440

Sorghum

Polynucleotide
SEQ ID NO: 613

bicolor

Polypeptide
SEQ ID NO: 614

Genomic
SEQ ID NO: 3711

Sb02g039410

Sorghum

Polynucleotide
SEQ ID NO: 615

bicolor

Polypeptide
SEQ ID NO: 616

Genomic
SEQ ID NO: 3712

Sb01g039740

Sorghum

Polynucleotide
SEQ ID NO: 617

bicolor

Polypeptide
SEQ ID NO: 618

Genomic
SEQ ID NO: 3713

Sb04g020690

Sorghum

Polynucleotide
SEQ ID NO: 619

bicolor

Polypeptide
SEQ ID NO: 620

Genomic
SEQ ID NO: 3714

Sb04g002190

Sorghum

Polynucleotide
SEQ ID NO: 621

bicolor

Polypeptide
SEQ ID NO: 622

Genomic
SEQ ID NO: 3715

Sb09g028680

Sorghum

Polynucleotide
SEQ ID NO: 623

bicolor

Polypeptide
SEQ ID NO: 624

Genomic
SEQ ID NO: 3716

dpzm08g032000

Zea mays

Polynucleotide
SEQ ID NO: 625

Polypeptide
SEQ ID NO: 626

Genomic
SEQ ID NO: 3717

Sb03g041600

Sorghum

Polynucleotide
SEQ ID NO: 627

bicolor

Polypeptide
SEQ ID NO: 628

Genomic
SEQ ID NO: 3718

Sb06g013820

Sorghum

Polynucleotide
SEQ ID NO: 629

bicolor

Polypeptide
SEQ ID NO: 630

Genomic
SEQ ID NO: 3719

Sb03g023990

Sorghum

Polynucleotide
SEQ ID NO: 631

bicolor

Polypeptide
SEQ ID NO: 632

Genomic
SEQ ID NO: 3720

Sb03g042970

Sorghum

Polynucleotide
SEQ ID NO: 633

bicolor

Polypeptide
SEQ ID NO: 634

Genomic
SEQ ID NO: 3721

Sb06g006920

Sorghum

Polynucleotide
SEQ ID NO: 635

bicolor

Polypeptide
SEQ ID NO: 636

Genomic
SEQ ID NO: 3722

Sb06g024150

Sorghum

Polynucleotide
SEQ ID NO: 637

bicolor

Polypeptide
SEQ ID NO: 638

Genomic
SEQ ID NO: 3723

dpzm06g048910

Zea mays

Polynucleotide
SEQ ID NO: 639

Polypeptide
SEQ ID NO: 640

Genomic
SEQ ID NO: 3724

Sb09g028680

Sorghum

Polynucleotide
SEQ ID NO: 641

bicolor

Polypeptide
SEQ ID NO: 642

Genomic
SEQ ID NO: 3725

Sb01g032930

Sorghum

Polynucleotide
SEQ ID NO: 643

bicolor

Polypeptide
SEQ ID NO: 644

Genomic
SEQ ID NO: 3726

Sb02g039570

Sorghum

Polynucleotide
SEQ ID NO: 645

bicolor

Polypeptide
SEQ ID NO: 646

Genomic
SEQ ID NO: 3727

Sb05g025900

Sorghum

Polynucleotide
SEQ ID NO: 647

bicolor

Polypeptide
SEQ ID NO: 648

Genomic
SEQ ID NO: 3728

Sb03g036480

Sorghum

Polynucleotide
SEQ ID NO: 649

bicolor

Polypeptide
SEQ ID NO: 650

Genomic
SEQ ID NO: 3729

dpzm00g103627

Zea mays

Polynucleotide
SEQ ID NO: 651

Polypeptide
SEQ ID NO: 652

Genomic
SEQ ID NO: 3730

Sb08g017080

Sorghum

Polynucleotide
SEQ ID NO: 653

bicolor

Polypeptide
SEQ ID NO: 654

Genomic
SEQ ID NO: 3731

Sb04g034520

Sorghum

Polynucleotide
SEQ ID NO: 655

bicolor

Polypeptide
SEQ ID NO: 656

Genomic
SEQ ID NO: 3732

Sb08g017660

Sorghum

Polynucleotide
SEQ ID NO: 657

bicolor

Polypeptide
SEQ ID NO: 658

Genomic
SEQ ID NO: 3733

Sb03g036580

Sorghum

Polynucleotide
SEQ ID NO: 659

bicolor

Polypeptide
SEQ ID NO: 660

Genomic
SEQ ID NO: 3734

Sb02g009340

Sorghum

Polynucleotide
SEQ ID NO: 661

bicolor

Polypeptide
SEQ ID NO: 662

Genomic
SEQ ID NO: 3735

Sb07g021290

Sorghum

Polynucleotide
SEQ ID NO: 663

bicolor

Polypeptide
SEQ ID NO: 664

Genomic
SEQ ID NO: 3736

Sb03g039790

Sorghum

Polynucleotide
SEQ ID NO: 665

bicolor

Polypeptide
SEQ ID NO: 666

Genomic
SEQ ID NO: 3737

Sb06g032000

Sorghum

Polynucleotide
SEQ ID NO: 667

bicolor

Polypeptide
SEQ ID NO: 668

Genomic
SEQ ID NO: 3738

Sb09g029126

Sorghum

Polynucleotide
SEQ ID NO: 669

bicolor

Polypeptide
SEQ ID NO: 670

Genomic
SEQ ID NO: 3739

Sb02g024620

Sorghum

Polynucleotide
SEQ ID NO: 671

bicolor

Polypeptide
SEQ ID NO: 672

Genomic
SEQ ID NO: 3740

Sb01g041100

Sorghum

Polynucleotide
SEQ ID NO: 673

bicolor

Polypeptide
SEQ ID NO: 674

Genomic
SEQ ID NO: 3741

Sb01g038910

Sorghum

Polynucleotide
SEQ ID NO: 675

bicolor

Polypeptide
SEQ ID NO: 676

Genomic
SEQ ID NO: 3742

Sb03g036480

Sorghum

Polynucleotide
SEQ ID NO: 677

bicolor

Polypeptide
SEQ ID NO: 678

Genomic
SEQ ID NO: 3743

Sb08g021375

Sorghum

Polynucleotide
SEQ ID NO: 679

bicolor

Polypeptide
SEQ ID NO: 680

Genomic
SEQ ID NO: 3744

Sb02g028255

Sorghum

Polynucleotide
SEQ ID NO: 681

bicolor

Polypeptide
SEQ ID NO: 682

Genomic
SEQ ID NO: 3745

Sb04g020470

Sorghum

Polynucleotide
SEQ ID NO: 683

bicolor

Polypeptide
SEQ ID NO: 684

Genomic
SEQ ID NO: 3746

Sb06g030230

Sorghum

Polynucleotide
SEQ ID NO: 685

bicolor

Polypeptide
SEQ ID NO: 686

Genomic
SEQ ID NO: 3747

Sb03g041580

Sorghum

Polynucleotide
SEQ ID NO: 687

bicolor

Polypeptide
SEQ ID NO: 688

Genomic
SEQ ID NO: 3748

Sb01g048640

Sorghum

Polynucleotide
SEQ ID NO: 689

bicolor

Polypeptide
SEQ ID NO: 690

Genomic
SEQ ID NO: 3749

Sb01g026405

Sorghum

Polynucleotide
SEQ ID NO: 691

bicolor

Polypeptide
SEQ ID NO: 692

Genomic
SEQ ID NO: 3750

Sb05g004850

Sorghum

Polynucleotide
SEQ ID NO: 693

bicolor

Polypeptide
SEQ ID NO: 694

Genomic
SEQ ID NO: 3751

Sb09g017570

Sorghum

Polynucleotide
SEQ ID NO: 695

bicolor

Polypeptide
SEQ ID NO: 696

Genomic
SEQ ID NO: 3752

Sb01g038910

Sorghum

Polynucleotide
SEQ ID NO: 697

bicolor

Polypeptide
SEQ ID NO: 698

Genomic
SEQ ID NO: 3753

Sb09g021610

Sorghum

Polynucleotide
SEQ ID NO: 699

bicolor

Polypeptide
SEQ ID NO: 700

Genomic
SEQ ID NO: 3754

Sb07g028600

Sorghum

Polynucleotide
SEQ ID NO: 701

bicolor

Polypeptide
SEQ ID NO: 702

Genomic
SEQ ID NO: 3755

Sb10g022110

Sorghum

Polynucleotide
SEQ ID NO: 703

bicolor

Polypeptide
SEQ ID NO: 704

Genomic
SEQ ID NO: 3756

Sb02g032815

Sorghum

Polynucleotide
SEQ ID NO: 705

bicolor

Polypeptide
SEQ ID NO: 706

Genomic
SEQ ID NO: 3757

Sb08g002690

Sorghum

Polynucleotide
SEQ ID NO: 707

bicolor

Polypeptide
SEQ ID NO: 708

Genomic
SEQ ID NO: 3758

Sb04g009200

Sorghum

Polynucleotide
SEQ ID NO: 709

bicolor

Polypeptide
SEQ ID NO: 710

Genomic
SEQ ID NO: 3759

Sb01g045060

Sorghum

Polynucleotide
SEQ ID NO: 711

bicolor

Polypeptide
SEQ ID NO: 712

Genomic
SEQ ID NO: 3760

Sb09g022260

Sorghum

Polynucleotide
SEQ ID NO: 713

bicolor

Polypeptide
SEQ ID NO: 714

Genomic
SEQ ID NO: 3761

Sb04g007280

Sorghum

Polynucleotide
SEQ ID NO: 715

bicolor

Polypeptide
SEQ ID NO: 716

Genomic
SEQ ID NO: 3762

Sb09g018630

Sorghum

Polynucleotide
SEQ ID NO: 717

bicolor

Polypeptide
SEQ ID NO: 718

Genomic
SEQ ID NO: 3763

Sb03g031420

Sorghum

Polynucleotide
SEQ ID NO: 719

bicolor

Polypeptide
SEQ ID NO: 720

Genomic
SEQ ID NO: 3764

Sb06g033030

Sorghum

Polynucleotide
SEQ ID NO: 721

bicolor

Polypeptide
SEQ ID NO: 722

Genomic
SEQ ID NO: 3765

Sb06g030740

Sorghum

Polynucleotide
SEQ ID NO: 723

bicolor

Polypeptide
SEQ ID NO: 724

Genomic
SEQ ID NO: 3766

Sb09g020780

Sorghum

Polynucleotide
SEQ ID NO: 725

bicolor

Polypeptide
SEQ ID NO: 726

Genomic
SEQ ID NO: 3767

Sb03g004390

Sorghum

Polynucleotide
SEQ ID NO: 727

bicolor

Polypeptide
SEQ ID NO: 728

Genomic
SEQ ID NO: 3768

Sb10g007830

Sorghum

Polynucleotide
SEQ ID NO: 729

bicolor

Polypeptide
SEQ ID NO: 730

Genomic
SEQ ID NO: 3769

Sb03g042820

Sorghum

Polynucleotide
SEQ ID NO: 731

bicolor

Polypeptide
SEQ ID NO: 732

Genomic
SEQ ID NO: 3770

Sb09g029600

Sorghum

Polynucleotide
SEQ ID NO: 733

bicolor

Polypeptide
SEQ ID NO: 734

Genomic
SEQ ID NO: 3771

Sb0010s003120

Sorghum

Polynucleotide
SEQ ID NO: 735

bicolor

Polypeptide
SEQ ID NO: 736

Genomic
SEQ ID NO: 3772

Sb0010s012040

Sorghum

Polynucleotide
SEQ ID NO: 737

bicolor

Polypeptide
SEQ ID NO: 738

Genomic
SEQ ID NO: 3773

Sb0012s010440

Sorghum

Polynucleotide
SEQ ID NO: 739

bicolor

Polypeptide
SEQ ID NO: 740

Genomic
SEQ ID NO: 3774

Sb0013s011130

Sorghum

Polynucleotide
SEQ ID NO: 741

bicolor

Polypeptide
SEQ ID NO: 742

Genomic
SEQ ID NO: 3775

Sb0059s003070

Sorghum

Polynucleotide
SEQ ID NO: 743

bicolor

Polypeptide
SEQ ID NO: 744

Genomic
SEQ ID NO: 3776

Sb0073s002030

Sorghum

Polynucleotide
SEQ ID NO: 745

bicolor

Polypeptide
SEQ ID NO: 746

Genomic
SEQ ID NO: 3777

Sb0073s002040

Sorghum

Polynucleotide
SEQ ID NO: 747

bicolor

Polypeptide
SEQ ID NO: 748

Genomic
SEQ ID NO: 3778

Sb01g000255

Sorghum

Polynucleotide
SEQ ID NO: 749

bicolor

Polypeptide
SEQ ID NO: 750

Genomic
SEQ ID NO: 3779

Sb01g000430

Sorghum

Polynucleotide
SEQ ID NO: 751

bicolor

Polypeptide
SEQ ID NO: 752

Genomic
SEQ ID NO: 3780

Sb01g000550

Sorghum

Polynucleotide
SEQ ID NO: 753

bicolor

Polypeptide
SEQ ID NO: 754

Genomic
SEQ ID NO: 3781

Sb01g000725

Sorghum

Polynucleotide
SEQ ID NO: 755

bicolor

Polypeptide
SEQ ID NO: 756

Genomic
SEQ ID NO: 3782

Sb01g001140

Sorghum

Polynucleotide
SEQ ID NO: 757

bicolor

Polypeptide
SEQ ID NO: 758

Genomic
SEQ ID NO: 3783

Sb01g004400

Sorghum

Polynucleotide
SEQ ID NO: 759

bicolor

Polypeptide
SEQ ID NO: 760

Genomic
SEQ ID NO: 3784

Sb01g001630

Sorghum

Polynucleotide
SEQ ID NO: 761

bicolor

Polypeptide
SEQ ID NO: 762

Genomic
SEQ ID NO: 3785

Sb01g004670

Sorghum

Polynucleotide
SEQ ID NO: 763

bicolor

Polypeptide
SEQ ID NO: 764

Genomic
SEQ ID NO: 3786

Sb01g002130

Sorghum

Polynucleotide
SEQ ID NO: 765

bicolor

Polypeptide
SEQ ID NO: 766

Genomic
SEQ ID NO: 3787

Sb01g002240

Sorghum

Polynucleotide
SEQ ID NO: 767

bicolor

Polypeptide
SEQ ID NO: 768

Genomic
SEQ ID NO: 3788

Sb01g005470

Sorghum

Polynucleotide
SEQ ID NO: 769

bicolor

Polypeptide
SEQ ID NO: 770

Genomic
SEQ ID NO: 3789

Sb01g002520

Sorghum

Polynucleotide
SEQ ID NO: 771

bicolor

Polypeptide
SEQ ID NO: 772

Genomic
SEQ ID NO: 3790

Sb01g002660

Sorghum

Polynucleotide
SEQ ID NO: 773

bicolor

Polypeptide
SEQ ID NO: 774

Genomic
SEQ ID NO: 3791

Sb01g002760

Sorghum

Polynucleotide
SEQ ID NO: 775

bicolor

Polypeptide
SEQ ID NO: 776

Genomic
SEQ ID NO: 3792

Sb01g002780

Sorghum

Polynucleotide
SEQ ID NO: 777

bicolor

Polypeptide
SEQ ID NO: 778

Genomic
SEQ ID NO: 3793

Sb01g003210

Sorghum

Polynucleotide
SEQ ID NO: 779

bicolor

Polypeptide
SEQ ID NO: 780

Genomic
SEQ ID NO: 3794

Sb01g003330

Sorghum

Polynucleotide
SEQ ID NO: 781

bicolor

Polypeptide
SEQ ID NO: 782

Genomic
SEQ ID NO: 3795

Sb01g003430

Sorghum

Polynucleotide
SEQ ID NO: 783

bicolor

Polypeptide
SEQ ID NO: 784

Genomic
SEQ ID NO: 3796

Sb01g003740

Sorghum

Polynucleotide
SEQ ID NO: 785

bicolor

Polypeptide
SEQ ID NO: 786

Genomic
SEQ ID NO: 3797

Sb01g003840

Sorghum

Polynucleotide
SEQ ID NO: 787

bicolor

Polypeptide
SEQ ID NO: 788

Genomic
SEQ ID NO: 3798

Sb01g003850

Sorghum

Polynucleotide
SEQ ID NO: 789

bicolor

Polypeptide
SEQ ID NO: 790

Genomic
SEQ ID NO: 3799

Sb01g003960

Sorghum

Polynucleotide
SEQ ID NO: 791

bicolor

Polypeptide
SEQ ID NO: 792

Genomic
SEQ ID NO: 3800

Sb01g004060

Sorghum

Polynucleotide
SEQ ID NO: 793

bicolor

Polypeptide
SEQ ID NO: 794

Genomic
SEQ ID NO: 3801

Sb01g004240

Sorghum

Polynucleotide
SEQ ID NO: 795

bicolor

Polypeptide
SEQ ID NO: 796

Genomic
SEQ ID NO: 3802

Sb01g004330

Sorghum

Polynucleotide
SEQ ID NO: 797

bicolor

Polypeptide
SEQ ID NO: 798

Genomic
SEQ ID NO: 3803

Sb01g004360

Sorghum

Polynucleotide
SEQ ID NO: 799

bicolor

Polypeptide
SEQ ID NO: 800

Genomic
SEQ ID NO: 3804

Sb01g004400

Sorghum

Polynucleotide
SEQ ID NO: 801

bicolor

Polypeptide
SEQ ID NO: 802

Genomic
SEQ ID NO: 3805

Sb01g004550

Sorghum

Polynucleotide
SEQ ID NO: 803

bicolor

Polypeptide
SEQ ID NO: 804

Genomic
SEQ ID NO: 3806

Sb01g004950

Sorghum

Polynucleotide
SEQ ID NO: 805

bicolor

Polypeptide
SEQ ID NO: 806

Genomic
SEQ ID NO: 3807

Sb01g004980

Sorghum

Polynucleotide
SEQ ID NO: 807

bicolor

Polypeptide
SEQ ID NO: 808

Genomic
SEQ ID NO: 3808

Sb01g005070

Sorghum

Polynucleotide
SEQ ID NO: 809

bicolor

Polypeptide
SEQ ID NO: 810

Genomic
SEQ ID NO: 3809

Sb01g005110

Sorghum

Polynucleotide
SEQ ID NO: 811

bicolor

Polypeptide
SEQ ID NO: 812

Genomic
SEQ ID NO: 3810

Sb01g005400

Sorghum

Polynucleotide
SEQ ID NO: 813

bicolor

Polypeptide
SEQ ID NO: 814

Genomic
SEQ ID NO: 3811

Sb01g005420

Sorghum

Polynucleotide
SEQ ID NO: 815

bicolor

Polypeptide
SEQ ID NO: 816

Genomic
SEQ ID NO: 3812

Sb01g005650

Sorghum

Polynucleotide
SEQ ID NO: 817

bicolor

Polypeptide
SEQ ID NO: 818

Genomic
SEQ ID NO: 3813

Sb01g006200

Sorghum

Polynucleotide
SEQ ID NO: 819

bicolor

Polypeptide
SEQ ID NO: 820

Genomic
SEQ ID NO: 3814

Sb01g006200

Sorghum

Polynucleotide
SEQ ID NO: 821

bicolor

Polypeptide
SEQ ID NO: 822

Genomic
SEQ ID NO: 3815

Sb01g006220

Sorghum

Polynucleotide
SEQ ID NO: 823

bicolor

Polypeptide
SEQ ID NO: 824

Genomic
SEQ ID NO: 3816

Sb01g006280

Sorghum

Polynucleotide
SEQ ID NO: 825

bicolor

Polypeptide
SEQ ID NO: 826

Genomic
SEQ ID NO: 3817

Sb01g006340

Sorghum

Polynucleotide
SEQ ID NO: 827

bicolor

Polypeptide
SEQ ID NO: 828

Genomic
SEQ ID NO: 3818

Sb01g006350

Sorghum

Polynucleotide
SEQ ID NO: 829

bicolor

Polypeptide
SEQ ID NO: 830

Genomic
SEQ ID NO: 3819

Sb01g006410

Sorghum

Polynucleotide
SEQ ID NO: 831

bicolor

Polypeptide
SEQ ID NO: 832

Genomic
SEQ ID NO: 3820

Sb01g006480

Sorghum

Polynucleotide
SEQ ID NO: 833

bicolor

Polypeptide
SEQ ID NO: 834

Genomic
SEQ ID NO: 3821

Sb01g006630

Sorghum

Polynucleotide
SEQ ID NO: 835

bicolor

Polypeptide
SEQ ID NO: 836

Genomic
SEQ ID NO: 3822

Sb01g006650

Sorghum

Polynucleotide
SEQ ID NO: 837

bicolor

Polypeptide
SEQ ID NO: 838

Genomic
SEQ ID NO: 3823

Sb01g012050

Sorghum

Polynucleotide
SEQ ID NO: 839

bicolor

Polypeptide
SEQ ID NO: 840

Genomic
SEQ ID NO: 3824

Sb01g007240

Sorghum

Polynucleotide
SEQ ID NO: 841

bicolor

Polypeptide
SEQ ID NO: 842

Genomic
SEQ ID NO: 3825

Sb01g007290

Sorghum

Polynucleotide
SEQ ID NO: 843

bicolor

Polypeptide
SEQ ID NO: 844

Genomic
SEQ ID NO: 3826

Sb01g007430

Sorghum

Polynucleotide
SEQ ID NO: 845

bicolor

Polypeptide
SEQ ID NO: 846

Genomic
SEQ ID NO: 3827

Sb01g007550

Sorghum

Polynucleotide
SEQ ID NO: 847

bicolor

Polypeptide
SEQ ID NO: 848

Genomic
SEQ ID NO: 3828

Sb01g007760

Sorghum

Polynucleotide
SEQ ID NO: 849

bicolor

Polypeptide
SEQ ID NO: 850

Genomic
SEQ ID NO: 3829

Sb01g007780

Sorghum

Polynucleotide
SEQ ID NO: 851

bicolor

Polypeptide
SEQ ID NO: 852

Genomic
SEQ ID NO: 3830

Sb01g007850

Sorghum

Polynucleotide
SEQ ID NO: 853

bicolor

Polypeptide
SEQ ID NO: 854

Genomic
SEQ ID NO: 3831

Sb01g008290

Sorghum

Polynucleotide
SEQ ID NO: 855

bicolor

Polypeptide
SEQ ID NO: 856

Genomic
SEQ ID NO: 3832

Sb10g005120

Sorghum

Polynucleotide
SEQ ID NO: 857

bicolor

Polypeptide
SEQ ID NO: 858

Genomic
SEQ ID NO: 3833

Sb01g008695

Sorghum

Polynucleotide
SEQ ID NO: 859

bicolor

Polypeptide
SEQ ID NO: 860

Genomic
SEQ ID NO: 3834

Sb01g008740

Sorghum

Polynucleotide
SEQ ID NO: 861

bicolor

Polypeptide
SEQ ID NO: 862

Genomic
SEQ ID NO: 3835

Sb01g009480

Sorghum

Polynucleotide
SEQ ID NO: 863

bicolor

Polypeptide
SEQ ID NO: 864

Genomic
SEQ ID NO: 3836

Sb01g009560

Sorghum

Polynucleotide
SEQ ID NO: 865

bicolor

Polypeptide
SEQ ID NO: 866

Genomic
SEQ ID NO: 3837

Sb01g009620

Sorghum

Polynucleotide
SEQ ID NO: 867

bicolor

Polypeptide
SEQ ID NO: 868

Genomic
SEQ ID NO: 3838

Sb01g009950

Sorghum

Polynucleotide
SEQ ID NO: 869

bicolor

Polypeptide
SEQ ID NO: 870

Genomic
SEQ ID NO: 3839

Sb01g009970

Sorghum

Polynucleotide
SEQ ID NO: 871

bicolor

Polypeptide
SEQ ID NO: 872

Genomic
SEQ ID NO: 3840

Sb01g010050

Sorghum

Polynucleotide
SEQ ID NO: 873

bicolor

Polypeptide
SEQ ID NO: 874

Genomic
SEQ ID NO: 3841

Sb01g010250

Sorghum

Polynucleotide
SEQ ID NO: 875

bicolor

Polypeptide
SEQ ID NO: 876

Genomic
SEQ ID NO: 3842

Sb01g010310

Sorghum

Polynucleotide
SEQ ID NO: 877

bicolor

Polypeptide
SEQ ID NO: 878

Genomic
SEQ ID NO: 3843

Sb01g010480

Sorghum

Polynucleotide
SEQ ID NO: 879

bicolor

Polypeptide
SEQ ID NO: 880

Genomic
SEQ ID NO: 3844

Sb01g010610

Sorghum

Polynucleotide
SEQ ID NO: 881

bicolor

Polypeptide
SEQ ID NO: 882

Genomic
SEQ ID NO: 3845

Sb01g010840

Sorghum

Polynucleotide
SEQ ID NO: 883

bicolor

Polypeptide
SEQ ID NO: 884

Genomic
SEQ ID NO: 3846

Sb01g010920

Sorghum

Polynucleotide
SEQ ID NO: 885

bicolor

Polypeptide
SEQ ID NO: 886

Genomic
SEQ ID NO: 3847

Sb01g010990

Sorghum

Polynucleotide
SEQ ID NO: 887

bicolor

Polypeptide
SEQ ID NO: 888

Genomic
SEQ ID NO: 3848

Sb01g018330

Sorghum

Polynucleotide
SEQ ID NO: 889

bicolor

Polypeptide
SEQ ID NO: 890

Genomic
SEQ ID NO: 3849

Sb01g011080

Sorghum

Polynucleotide
SEQ ID NO: 891

bicolor

Polypeptide
SEQ ID NO: 892

Genomic
SEQ ID NO: 3850

Sb01g011240

Sorghum

Polynucleotide
SEQ ID NO: 893

bicolor

Polypeptide
SEQ ID NO: 894

Genomic
SEQ ID NO: 3851

Sb01g011360

Sorghum

Polynucleotide
SEQ ID NO: 895

bicolor

Polypeptide
SEQ ID NO: 896

Genomic
SEQ ID NO: 3852

Sb01g011520

Sorghum

Polynucleotide
SEQ ID NO: 897

bicolor

Polypeptide
SEQ ID NO: 898

Genomic
SEQ ID NO: 3853

Sb01g019490

Sorghum

Polynucleotide
SEQ ID NO: 899

bicolor

Polypeptide
SEQ ID NO: 900

Genomic
SEQ ID NO: 3854

Sb01g011810

Sorghum

Polynucleotide
SEQ ID NO: 901

bicolor

Polypeptide
SEQ ID NO: 902

Genomic
SEQ ID NO: 3855

Sb01g012250

Sorghum

Polynucleotide
SEQ ID NO: 903

bicolor

Polypeptide
SEQ ID NO: 904

Genomic
SEQ ID NO: 3856

Sb01g012260

Sorghum

Polynucleotide
SEQ ID NO: 905

bicolor

Polypeptide
SEQ ID NO: 906

Genomic
SEQ ID NO: 3857

Sb01g012780

Sorghum

Polynucleotide
SEQ ID NO: 907

bicolor

Polypeptide
SEQ ID NO: 908

Genomic
SEQ ID NO: 3858

Sb01g013070

Sorghum

Polynucleotide
SEQ ID NO: 909

bicolor

Polypeptide
SEQ ID NO: 910

Genomic
SEQ ID NO: 3859

Sb01g013160

Sorghum

Polynucleotide
SEQ ID NO: 911

bicolor

Polypeptide
SEQ ID NO: 912

Genomic
SEQ ID NO: 3860

Sb01g013180

Sorghum

Polynucleotide
SEQ ID NO: 913

bicolor

Polypeptide
SEQ ID NO: 914

Genomic
SEQ ID NO: 3861

Sb01g013340

Sorghum

Polynucleotide
SEQ ID NO: 915

bicolor

Polypeptide
SEQ ID NO: 916

Genomic
SEQ ID NO: 3862

Sb01g013560

Sorghum

Polynucleotide
SEQ ID NO: 917

bicolor

Polypeptide
SEQ ID NO: 918

Genomic
SEQ ID NO: 3863

Sb01g013700

Sorghum

Polynucleotide
SEQ ID NO: 919

bicolor

Polypeptide
SEQ ID NO: 920

Genomic
SEQ ID NO: 3864

Sb01g013810

Sorghum

Polynucleotide
SEQ ID NO: 921

bicolor

Polypeptide
SEQ ID NO: 922

Genomic
SEQ ID NO: 3865

Sb01g014290

Sorghum

Polynucleotide
SEQ ID NO: 923

bicolor

Polypeptide
SEQ ID NO: 924

Genomic
SEQ ID NO: 3866

Sb01g014370

Sorghum

Polynucleotide
SEQ ID NO: 925

bicolor

Polypeptide
SEQ ID NO: 926

Genomic
SEQ ID NO: 3867

Sb01g014910

Sorghum

Polynucleotide
SEQ ID NO: 927

bicolor

Polypeptide
SEQ ID NO: 928

Genomic
SEQ ID NO: 3868

Sb01g025600

Sorghum

Polynucleotide
SEQ ID NO: 929

bicolor

Polypeptide
SEQ ID NO: 930

Genomic
SEQ ID NO: 3869

Sb01g025610

Sorghum

Polynucleotide
SEQ ID NO: 931

bicolor

Polypeptide
SEQ ID NO: 932

Genomic
SEQ ID NO: 3870

Sb01g015040

Sorghum

Polynucleotide
SEQ ID NO: 933

bicolor

Polypeptide
SEQ ID NO: 934

Genomic
SEQ ID NO: 3871

Sb01g015210

Sorghum

Polynucleotide
SEQ ID NO: 935

bicolor

Polypeptide
SEQ ID NO: 936

Genomic
SEQ ID NO: 3872

Sb01g015240

Sorghum

Polynucleotide
SEQ ID NO: 937

bicolor

Polypeptide
SEQ ID NO: 938

Genomic
SEQ ID NO: 3873

Sb07g008201

Sorghum

Polynucleotide
SEQ ID NO: 939

bicolor

Polypeptide
SEQ ID NO: 940

Genomic
SEQ ID NO: 3874

Sb01g015770

Sorghum

Polynucleotide
SEQ ID NO: 941

bicolor

Polypeptide
SEQ ID NO: 942

Genomic
SEQ ID NO: 3875

Sb01g015970

Sorghum

Polynucleotide
SEQ ID NO: 943

bicolor

Polypeptide
SEQ ID NO: 944

Genomic
SEQ ID NO: 3876

Sb01g016020

Sorghum

Polynucleotide
SEQ ID NO: 945

bicolor

Polypeptide
SEQ ID NO: 946

Genomic
SEQ ID NO: 3877

Sb01g016170

Sorghum

Polynucleotide
SEQ ID NO: 947

bicolor

Polypeptide
SEQ ID NO: 948

Genomic
SEQ ID NO: 3878

Sb01g016490

Sorghum

Polynucleotide
SEQ ID NO: 949

bicolor

Polypeptide
SEQ ID NO: 950

Genomic
SEQ ID NO: 3879

Sb01g016600

Sorghum

Polynucleotide
SEQ ID NO: 951

bicolor

Polypeptide
SEQ ID NO: 952

Genomic
SEQ ID NO: 3880

Sb01g030990

Sorghum

Polynucleotide
SEQ ID NO: 953

bicolor

Polypeptide
SEQ ID NO: 954

Genomic
SEQ ID NO: 3881

Sb01g017230

Sorghum

Polynucleotide
SEQ ID NO: 955

bicolor

Polypeptide
SEQ ID NO: 956

Genomic
SEQ ID NO: 3882

Sb01g017450

Sorghum

Polynucleotide
SEQ ID NO: 957

bicolor

Polypeptide
SEQ ID NO: 958

Genomic
SEQ ID NO: 3883

Sb01g017460

Sorghum

Polynucleotide
SEQ ID NO: 959

bicolor

Polypeptide
SEQ ID NO: 960

Genomic
SEQ ID NO: 3884

Sb01g017540

Sorghum

Polynucleotide
SEQ ID NO: 961

bicolor

Polypeptide
SEQ ID NO: 962

Genomic
SEQ ID NO: 3885

Sb01g017560

Sorghum

Polynucleotide
SEQ ID NO: 963

bicolor

Polypeptide
SEQ ID NO: 964

Genomic
SEQ ID NO: 3886

Sb01g032390

Sorghum

Polynucleotide
SEQ ID NO: 965

bicolor

Polypeptide
SEQ ID NO: 966

Genomic
SEQ ID NO: 3887

Sb01g017720

Sorghum

Polynucleotide
SEQ ID NO: 967

bicolor

Polypeptide
SEQ ID NO: 968

Genomic
SEQ ID NO: 3888

Sb01g018600

Sorghum

Polynucleotide
SEQ ID NO: 969

bicolor

Polypeptide
SEQ ID NO: 970

Genomic
SEQ ID NO: 3889

Sb01g018700

Sorghum

Polynucleotide
SEQ ID NO: 971

bicolor

Polypeptide
SEQ ID NO: 972

Genomic
SEQ ID NO: 3890

Sb01g018910

Sorghum

Polynucleotide
SEQ ID NO: 973

bicolor

Polypeptide
SEQ ID NO: 974

Genomic
SEQ ID NO: 3891

Sb01g018950

Sorghum

Polynucleotide
SEQ ID NO: 975

bicolor

Polypeptide
SEQ ID NO: 976

Genomic
SEQ ID NO: 3892

Sb01g019100

Sorghum

Polynucleotide
SEQ ID NO: 977

bicolor

Polypeptide
SEQ ID NO: 978

Genomic
SEQ ID NO: 3893

Sb01g019230

Sorghum

Polynucleotide
SEQ ID NO: 979

bicolor

Polypeptide
SEQ ID NO: 980

Genomic
SEQ ID NO: 3894

Sb01g019330

Sorghum

Polynucleotide
SEQ ID NO: 981

bicolor

Polypeptide
SEQ ID NO: 982

Genomic
SEQ ID NO: 3895

Sb01g019510

Sorghum

Polynucleotide
SEQ ID NO: 983

bicolor

Polypeptide
SEQ ID NO: 984

Genomic
SEQ ID NO: 3896

Sb01g019540

Sorghum

Polynucleotide
SEQ ID NO: 985

bicolor

Polypeptide
SEQ ID NO: 986

Genomic
SEQ ID NO: 3897

Sb01g019580

Sorghum

Polynucleotide
SEQ ID NO: 987

bicolor

Polypeptide
SEQ ID NO: 988

Genomic
SEQ ID NO: 3898

Sb01g019840

Sorghum

Polynucleotide
SEQ ID NO: 989

bicolor

Polypeptide
SEQ ID NO: 990

Genomic
SEQ ID NO: 3899

Sb01g019860

Sorghum

Polynucleotide
SEQ ID NO: 991

bicolor

Polypeptide
SEQ ID NO: 992

Genomic
SEQ ID NO: 3900

Sb01g019970

Sorghum

Polynucleotide
SEQ ID NO: 993

bicolor

Polypeptide
SEQ ID NO: 994

Genomic
SEQ ID NO: 3901

Sb01g020180

Sorghum

Polynucleotide
SEQ ID NO: 995

bicolor

Polypeptide
SEQ ID NO: 996

Genomic
SEQ ID NO: 3902

Sb01g020810

Sorghum

Polynucleotide
SEQ ID NO: 997

bicolor

Polypeptide
SEQ ID NO: 998

Genomic
SEQ ID NO: 3903

Sb01g045010

Sorghum

Polynucleotide
SEQ ID NO: 999

bicolor

Polypeptide
SEQ ID NO: 1000

Genomic
SEQ ID NO: 3904

Sb01g021030

Sorghum

Polynucleotide
SEQ ID NO: 1001

bicolor

Polypeptide
SEQ ID NO: 1002

Genomic
SEQ ID NO: 3905

Sb01g021080

Sorghum

Polynucleotide
SEQ ID NO: 1003

bicolor

Polypeptide
SEQ ID NO: 1004

Genomic
SEQ ID NO: 3906

Sb01g021680

Sorghum

Polynucleotide
SEQ ID NO: 1005

bicolor

Polypeptide
SEQ ID NO: 1006

Genomic
SEQ ID NO: 3907

Sb01g021760

Sorghum

Polynucleotide
SEQ ID NO: 1007

bicolor

Polypeptide
SEQ ID NO: 1008

Genomic
SEQ ID NO: 3908

Sb01g021890

Sorghum

Polynucleotide
SEQ ID NO: 1009

bicolor

Polypeptide
SEQ ID NO: 1010

Genomic
SEQ ID NO: 3909

Sb01g022210

Sorghum

Polynucleotide
SEQ ID NO: 1011

bicolor

Polypeptide
SEQ ID NO: 1012

Genomic
SEQ ID NO: 3910

Sb01g080950

Sorghum

Polynucleotide
SEQ ID NO: 1013

bicolor

Polypeptide
SEQ ID NO: 1014

Genomic
SEQ ID NO: 3911

Sb01g025290

Sorghum

Polynucleotide
SEQ ID NO: 1015

bicolor

Polypeptide
SEQ ID NO: 1016

Genomic
SEQ ID NO: 3912

Sb01g025310

Sorghum

Polynucleotide
SEQ ID NO: 1017

bicolor

Polypeptide
SEQ ID NO: 1018

Genomic
SEQ ID NO: 3913

Sb01g026660

Sorghum

Polynucleotide
SEQ ID NO: 1019

bicolor

Polypeptide
SEQ ID NO: 1020

Genomic
SEQ ID NO: 3914

Sb01g026700

Sorghum

Polynucleotide
SEQ ID NO: 1021

bicolor

Polypeptide
SEQ ID NO: 1022

Genomic
SEQ ID NO: 3915

Sb01g027010

Sorghum

Polynucleotide
SEQ ID NO: 1023

bicolor

Polypeptide
SEQ ID NO: 1024

Genomic
SEQ ID NO: 3916

Sb01g027250

Sorghum

Polynucleotide
SEQ ID NO: 1025

bicolor

Polypeptide
SEQ ID NO: 1026

Genomic
SEQ ID NO: 3917

Sb01g110910

Sorghum

Polynucleotide
SEQ ID NO: 1027

bicolor

Polypeptide
SEQ ID NO: 1028

Genomic
SEQ ID NO: 3918

Sb01g027330

Sorghum

Polynucleotide
SEQ ID NO: 1029

bicolor

Polypeptide
SEQ ID NO: 1030

Genomic
SEQ ID NO: 3919

Sb01g027490

Sorghum

Polynucleotide
SEQ ID NO: 1031

bicolor

Polypeptide
SEQ ID NO: 1032

Genomic
SEQ ID NO: 3920

Sb01g027680

Sorghum

Polynucleotide
SEQ ID NO: 1033

bicolor

Polypeptide
SEQ ID NO: 1034

Genomic
SEQ ID NO: 3921

Sb01g027790

Sorghum

Polynucleotide
SEQ ID NO: 1035

bicolor

Polypeptide
SEQ ID NO: 1036

Genomic
SEQ ID NO: 3922

Sb01g027920

Sorghum

Polynucleotide
SEQ ID NO: 1037

bicolor

Polypeptide
SEQ ID NO: 1038

Genomic
SEQ ID NO: 3923

Sb01g028100

Sorghum

Polynucleotide
SEQ ID NO: 1039

bicolor

Polypeptide
SEQ ID NO: 1040

Genomic
SEQ ID NO: 3924

Sb01g028280

Sorghum

Polynucleotide
SEQ ID NO: 1041

bicolor

Polypeptide
SEQ ID NO: 1042

Genomic
SEQ ID NO: 3925

Sb01g028340

Sorghum

Polynucleotide
SEQ ID NO: 1043

bicolor

Polypeptide
SEQ ID NO: 1044

Genomic
SEQ ID NO: 3926

Sb01g117430

Sorghum

Polynucleotide
SEQ ID NO: 1045

bicolor

Polypeptide
SEQ ID NO: 1046

Genomic
SEQ ID NO: 3927

Sb01g028390

Sorghum

Polynucleotide
SEQ ID NO: 1047

bicolor

Polypeptide
SEQ ID NO: 1048

Genomic
SEQ ID NO: 3928

Sb01g028760

Sorghum

Polynucleotide
SEQ ID NO: 1049

bicolor

Polypeptide
SEQ ID NO: 1050

Genomic
SEQ ID NO: 3929

Sb01g028770

Sorghum

Polynucleotide
SEQ ID NO: 1051

bicolor

Polypeptide
SEQ ID NO: 1052

Genomic
SEQ ID NO: 3930

Sb01g029020

Sorghum

Polynucleotide
SEQ ID NO: 1053

bicolor

Polypeptide
SEQ ID NO: 1054

Genomic
SEQ ID NO: 3931

Sb01g029250

Sorghum

Polynucleotide
SEQ ID NO: 1055

bicolor

Polypeptide
SEQ ID NO: 1056

Genomic
SEQ ID NO: 3932

Sb01g029350

Sorghum

Polynucleotide
SEQ ID NO: 1057

bicolor

Polypeptide
SEQ ID NO: 1058

Genomic
SEQ ID NO: 3933

Sb01g029550

Sorghum

Polynucleotide
SEQ ID NO: 1059

bicolor

Polypeptide
SEQ ID NO: 1060

Genomic
SEQ ID NO: 3934

Sb01g031335

Sorghum

Polynucleotide
SEQ ID NO: 1061

bicolor

Polypeptide
SEQ ID NO: 1062

Genomic
SEQ ID NO: 3935

Sb01g031340

Sorghum

Polynucleotide
SEQ ID NO: 1063

bicolor

Polypeptide
SEQ ID NO: 1064

Genomic
SEQ ID NO: 3936

Sb01g031580

Sorghum

Polynucleotide
SEQ ID NO: 1065

bicolor

Polypeptide
SEQ ID NO: 1066

Genomic
SEQ ID NO: 3937

Sb01g031920

Sorghum

Polynucleotide
SEQ ID NO: 1067

bicolor

Polypeptide
SEQ ID NO: 1068

Genomic
SEQ ID NO: 3938

Sb01g126250

Sorghum

Polynucleotide
SEQ ID NO: 1069

bicolor

Polypeptide
SEQ ID NO: 1070

Genomic
SEQ ID NO: 3939

Sb01g032360

Sorghum

Polynucleotide
SEQ ID NO: 1071

bicolor

Polypeptide
SEQ ID NO: 1072

Genomic
SEQ ID NO: 3940

Sb01g032875

Sorghum

Polynucleotide
SEQ ID NO: 1073

bicolor

Polypeptide
SEQ ID NO: 1074

Genomic
SEQ ID NO: 3941

Sb01g033250

Sorghum

Polynucleotide
SEQ ID NO: 1075

bicolor

Polypeptide
SEQ ID NO: 1076

Genomic
SEQ ID NO: 3942

Sb01g033340

Sorghum

Polynucleotide
SEQ ID NO: 1077

bicolor

Polypeptide
SEQ ID NO: 1078

Genomic
SEQ ID NO: 3943

Sb01g129200

Sorghum

Polynucleotide
SEQ ID NO: 1079

bicolor

Polypeptide
SEQ ID NO: 1080

Genomic
SEQ ID NO: 3944

Sb01g129450

Sorghum

Polynucleotide
SEQ ID NO: 1081

bicolor

Polypeptide
SEQ ID NO: 1082

Genomic
SEQ ID NO: 3945

Sb01g033620

Sorghum

Polynucleotide
SEQ ID NO: 1083

bicolor

Polypeptide
SEQ ID NO: 1084

Genomic
SEQ ID NO: 3946

Sb01g033880

Sorghum

Polynucleotide
SEQ ID NO: 1085

bicolor

Polypeptide
SEQ ID NO: 1086

Genomic
SEQ ID NO: 3947

Sb01g034290

Sorghum

Polynucleotide
SEQ ID NO: 1087

bicolor

Polypeptide
SEQ ID NO: 1088

Genomic
SEQ ID NO: 3948

Sb01g034300

Sorghum

Polynucleotide
SEQ ID NO: 1089

bicolor

Polypeptide
SEQ ID NO: 1090

Genomic
SEQ ID NO: 3949

Sb01g034390

Sorghum

Polynucleotide
SEQ ID NO: 1091

bicolor

Polypeptide
SEQ ID NO: 1092

Genomic
SEQ ID NO: 3950

Sb01g034540

Sorghum

Polynucleotide
SEQ ID NO: 1093

bicolor

Polypeptide
SEQ ID NO: 1094

Genomic
SEQ ID NO: 3951

Sb01g034710

Sorghum

Polynucleotide
SEQ ID NO: 1095

bicolor

Polypeptide
SEQ ID NO: 1096

Genomic
SEQ ID NO: 3952

Sb01g034890

Sorghum

Polynucleotide
SEQ ID NO: 1097

bicolor

Polypeptide
SEQ ID NO: 1098

Genomic
SEQ ID NO: 3953

Sb01g131900

Sorghum

Polynucleotide
SEQ ID NO: 1099

bicolor

Polypeptide
SEQ ID NO: 1100

Genomic
SEQ ID NO: 3954

Sb09g004883

Sorghum

Polynucleotide
SEQ ID NO: 1101

bicolor

Polypeptide
SEQ ID NO: 1102

Genomic
SEQ ID NO: 3955

Sb01g035860

Sorghum

Polynucleotide
SEQ ID NO: 1103

bicolor

Polypeptide
SEQ ID NO: 1104

Genomic
SEQ ID NO: 3956

Sb01g036180

Sorghum

Polynucleotide
SEQ ID NO: 1105

bicolor

Polypeptide
SEQ ID NO: 1106

Genomic
SEQ ID NO: 3957

Sb01g036220

Sorghum

Polynucleotide
SEQ ID NO: 1107

bicolor

Polypeptide
SEQ ID NO: 1108

Genomic
SEQ ID NO: 3958

Sb01g036350

Sorghum

Polynucleotide
SEQ ID NO: 1109

bicolor

Polypeptide
SEQ ID NO: 1110

Genomic
SEQ ID NO: 3959

Sb01g037380

Sorghum

Polynucleotide
SEQ ID NO: 1111

bicolor

Polypeptide
SEQ ID NO: 1112

Genomic
SEQ ID NO: 3960

Sb01g037420

Sorghum

Polynucleotide
SEQ ID NO: 1113

bicolor

Polypeptide
SEQ ID NO: 1114

Genomic
SEQ ID NO: 3961

Sb01g037510

Sorghum

Polynucleotide
SEQ ID NO: 1115

bicolor

Polypeptide
SEQ ID NO: 1116

Genomic
SEQ ID NO: 3962

Sb01g037710

Sorghum

Polynucleotide
SEQ ID NO: 1117

bicolor

Polypeptide
SEQ ID NO: 1118

Genomic
SEQ ID NO: 3963

Sb01g037720

Sorghum

Polynucleotide
SEQ ID NO: 1119

bicolor

Polypeptide
SEQ ID NO: 1120

Genomic
SEQ ID NO: 3964

Sb01g037890

Sorghum

Polynucleotide
SEQ ID NO: 1121

bicolor

Polypeptide
SEQ ID NO: 1122

Genomic
SEQ ID NO: 3965

Sb01g037900

Sorghum

Polynucleotide
SEQ ID NO: 1123

bicolor

Polypeptide
SEQ ID NO: 1124

Genomic
SEQ ID NO: 3966

Sb01g137540

Sorghum

Polynucleotide
SEQ ID NO: 1125

bicolor

Polypeptide
SEQ ID NO: 1126

Genomic
SEQ ID NO: 3967

Sb01g038010

Sorghum

Polynucleotide
SEQ ID NO: 1127

bicolor

Polypeptide
SEQ ID NO: 1128

Genomic
SEQ ID NO: 3968

Sb01g038070

Sorghum

Polynucleotide
SEQ ID NO: 1129

bicolor

Polypeptide
SEQ ID NO: 1130

Genomic
SEQ ID NO: 3969

Sb01g038160

Sorghum

Polynucleotide
SEQ ID NO: 1131

bicolor

Polypeptide
SEQ ID NO: 1132

Genomic
SEQ ID NO: 3970

Sb01g038300

Sorghum

Polynucleotide
SEQ ID NO: 1133

bicolor

Polypeptide
SEQ ID NO: 1134

Genomic
SEQ ID NO: 3971

Sb01g038350

Sorghum

Polynucleotide
SEQ ID NO: 1135

bicolor

Polypeptide
SEQ ID NO: 1136

Genomic
SEQ ID NO: 3972

Sb01g038400

Sorghum

Polynucleotide
SEQ ID NO: 1137

bicolor

Polypeptide
SEQ ID NO: 1138

Genomic
SEQ ID NO: 3973

Sb01g038800

Sorghum

Polynucleotide
SEQ ID NO: 1139

bicolor

Polypeptide
SEQ ID NO: 1140

Genomic
SEQ ID NO: 3974

Sb01g038830

Sorghum

Polynucleotide
SEQ ID NO: 1141

bicolor

Polypeptide
SEQ ID NO: 1142

Genomic
SEQ ID NO: 3975

Sb01g039010

Sorghum

Polynucleotide
SEQ ID NO: 1143

bicolor

Polypeptide
SEQ ID NO: 1144

Genomic
SEQ ID NO: 3976

Sb01g039230

Sorghum

Polynucleotide
SEQ ID NO: 1145

bicolor

Polypeptide
SEQ ID NO: 1146

Genomic
SEQ ID NO: 3977

Sb01g039250

Sorghum

Polynucleotide
SEQ ID NO: 1147

bicolor

Polypeptide
SEQ ID NO: 1148

Genomic
SEQ ID NO: 3978

Sb01g039550

Sorghum

Polynucleotide
SEQ ID NO: 1149

bicolor

Polypeptide
SEQ ID NO: 1150

Genomic
SEQ ID NO: 3979

Sb01g039710

Sorghum

Polynucleotide
SEQ ID NO: 1151

bicolor

Polypeptide
SEQ ID NO: 1152

Genomic
SEQ ID NO: 3980

Sb01g039720

Sorghum

Polynucleotide
SEQ ID NO: 1153

bicolor

Polypeptide
SEQ ID NO: 1154

Genomic
SEQ ID NO: 3981

Sb01g039830

Sorghum

Polynucleotide
SEQ ID NO: 1155

bicolor

Polypeptide
SEQ ID NO: 1156

Genomic
SEQ ID NO: 3982

Sb01g040110

Sorghum

Polynucleotide
SEQ ID NO: 1157

bicolor

Polypeptide
SEQ ID NO: 1158

Genomic
SEQ ID NO: 3983

Sb01g040430

Sorghum

Polynucleotide
SEQ ID NO: 1159

bicolor

Polypeptide
SEQ ID NO: 1160

Genomic
SEQ ID NO: 3984

Sb01g040660

Sorghum

Polynucleotide
SEQ ID NO: 1161

bicolor

Polypeptide
SEQ ID NO: 1162

Genomic
SEQ ID NO: 3985

Sb01g040960

Sorghum

Polynucleotide
SEQ ID NO: 1163

bicolor

Polypeptide
SEQ ID NO: 1164

Genomic
SEQ ID NO: 3986

Sb01g040980

Sorghum

Polynucleotide
SEQ ID NO: 1165

bicolor

Polypeptide
SEQ ID NO: 1166

Genomic
SEQ ID NO: 3987

Sb01g041120

Sorghum

Polynucleotide
SEQ ID NO: 1167

bicolor

Polypeptide
SEQ ID NO: 1168

Genomic
SEQ ID NO: 3988

Sb01g041230

Sorghum

Polynucleotide
SEQ ID NO: 1169

bicolor

Polypeptide
SEQ ID NO: 1170

Genomic
SEQ ID NO: 3989

Sb01g142330

Sorghum

Polynucleotide
SEQ ID NO: 1171

bicolor

Polypeptide
SEQ ID NO: 1172

Genomic
SEQ ID NO: 3990

Sb01g041480

Sorghum

Polynucleotide
SEQ ID NO: 1173

bicolor

Polypeptide
SEQ ID NO: 1174

Genomic
SEQ ID NO: 3991

Sb01g041850

Sorghum

Polynucleotide
SEQ ID NO: 1175

bicolor

Polypeptide
SEQ ID NO: 1176

Genomic
SEQ ID NO: 3992

Sb01g042200

Sorghum

Polynucleotide
SEQ ID NO: 1177

bicolor

Polypeptide
SEQ ID NO: 1178

Genomic
SEQ ID NO: 3993

Sb01g042230

Sorghum

Polynucleotide
SEQ ID NO: 1179

bicolor

Polypeptide
SEQ ID NO: 1180

Genomic
SEQ ID NO: 3994

Sb01g042450

Sorghum

Polynucleotide
SEQ ID NO: 1181

bicolor

Polypeptide
SEQ ID NO: 1182

Genomic
SEQ ID NO: 3995

Sb01g042490

Sorghum

Polynucleotide
SEQ ID NO: 1183

bicolor

Polypeptide
SEQ ID NO: 1184

Genomic
SEQ ID NO: 3996

Sb01g042735

Sorghum

Polynucleotide
SEQ ID NO: 1185

bicolor

Polypeptide
SEQ ID NO: 1186

Genomic
SEQ ID NO: 3997

Sb01g042840

Sorghum

Polynucleotide
SEQ ID NO: 1187

bicolor

Polypeptide
SEQ ID NO: 1188

Genomic
SEQ ID NO: 3998

Sb01g042890

Sorghum

Polynucleotide
SEQ ID NO: 1189

bicolor

Polypeptide
SEQ ID NO: 1190

Genomic
SEQ ID NO: 3999

Sb01g043190

Sorghum

Polynucleotide
SEQ ID NO: 1191

bicolor

Polypeptide
SEQ ID NO: 1192

Genomic
SEQ ID NO: 4000

Sb01g043280

Sorghum

Polynucleotide
SEQ ID NO: 1193

bicolor

Polypeptide
SEQ ID NO: 1194

Genomic
SEQ ID NO: 4001

Sb01g043340

Sorghum

Polynucleotide
SEQ ID NO: 1195

bicolor

Polypeptide
SEQ ID NO: 1196

Genomic
SEQ ID NO: 4002

Sb01g043370

Sorghum

Polynucleotide
SEQ ID NO: 1197

bicolor

Polypeptide
SEQ ID NO: 1198

Genomic
SEQ ID NO: 4003

Sb01g043420

Sorghum

Polynucleotide
SEQ ID NO: 1199

bicolor

Polypeptide
SEQ ID NO: 1200

Genomic
SEQ ID NO: 4004

Sb01g043570

Sorghum

Polynucleotide
SEQ ID NO: 1201

bicolor

Polypeptide
SEQ ID NO: 1202

Genomic
SEQ ID NO: 4005

Sb01g043840

Sorghum

Polynucleotide
SEQ ID NO: 1203

bicolor

Polypeptide
SEQ ID NO: 1204

Genomic
SEQ ID NO: 4006

Sb01g145860

Sorghum

Polynucleotide
SEQ ID NO: 1205

bicolor

Polypeptide
SEQ ID NO: 1206

Genomic
SEQ ID NO: 4007

Sb01g044100

Sorghum

Polynucleotide
SEQ ID NO: 1207

bicolor

Polypeptide
SEQ ID NO: 1208

Genomic
SEQ ID NO: 4008

Sb01g044180

Sorghum

Polynucleotide
SEQ ID NO: 1209

bicolor

Polypeptide
SEQ ID NO: 1210

Genomic
SEQ ID NO: 4009

Sb01g044340

Sorghum

Polynucleotide
SEQ ID NO: 1211

bicolor

Polypeptide
SEQ ID NO: 1212

Genomic
SEQ ID NO: 4010

Sb01g044450

Sorghum

Polynucleotide
SEQ ID NO: 1213

bicolor

Polypeptide
SEQ ID NO: 1214

Genomic
SEQ ID NO: 4011

Sb01g146630

Sorghum

Polynucleotide
SEQ ID NO: 1215

bicolor

Polypeptide
SEQ ID NO: 1216

Genomic
SEQ ID NO: 4012

Sb01g147260

Sorghum

Polynucleotide
SEQ ID NO: 1217

bicolor

Polypeptide
SEQ ID NO: 1218

Genomic
SEQ ID NO: 4013

Sb01g044910

Sorghum

Polynucleotide
SEQ ID NO: 1219

bicolor

Polypeptide
SEQ ID NO: 1220

Genomic
SEQ ID NO: 4014

Sb01g045110

Sorghum

Polynucleotide
SEQ ID NO: 1221

bicolor

Polypeptide
SEQ ID NO: 1222

Genomic
SEQ ID NO: 4015

Sb01g045380

Sorghum

Polynucleotide
SEQ ID NO: 1223

bicolor

Polypeptide
SEQ ID NO: 1224

Genomic
SEQ ID NO: 4016

Sb01g045390

Sorghum

Polynucleotide
SEQ ID NO: 1225

bicolor

Polypeptide
SEQ ID NO: 1226

Genomic
SEQ ID NO: 4017

Sb01g148370

Sorghum

Polynucleotide
SEQ ID NO: 1227

bicolor

Polypeptide
SEQ ID NO: 1228

Genomic
SEQ ID NO: 4018

Sb01g045850

Sorghum

Polynucleotide
SEQ ID NO: 1229

bicolor

Polypeptide
SEQ ID NO: 1230

Genomic
SEQ ID NO: 4019

Sb01g046040

Sorghum

Polynucleotide
SEQ ID NO: 1231

bicolor

Polypeptide
SEQ ID NO: 1232

Genomic
SEQ ID NO: 4020

Sb01g046160

Sorghum

Polynucleotide
SEQ ID NO: 1233

bicolor

Polypeptide
SEQ ID NO: 1234

Genomic
SEQ ID NO: 4021

Sb01g046210

Sorghum

Polynucleotide
SEQ ID NO: 1235

bicolor

Polypeptide
SEQ ID NO: 1236

Genomic
SEQ ID NO: 4022

Sb01g046520

Sorghum

Polynucleotide
SEQ ID NO: 1237

bicolor

Polypeptide
SEQ ID NO: 1238

Genomic
SEQ ID NO: 4023

Sb01g046550

Sorghum

Polynucleotide
SEQ ID NO: 1239

bicolor

Polypeptide
SEQ ID NO: 1240

Genomic
SEQ ID NO: 4024

Sb01g046980

Sorghum

Polynucleotide
SEQ ID NO: 1241

bicolor

Polypeptide
SEQ ID NO: 1242

Genomic
SEQ ID NO: 4025

Sb01g047170

Sorghum

Polynucleotide
SEQ ID NO: 1243

bicolor

Polypeptide
SEQ ID NO: 1244

Genomic
SEQ ID NO: 4026

Sb01g047620

Sorghum

Polynucleotide
SEQ ID NO: 1245

bicolor

Polypeptide
SEQ ID NO: 1246

Genomic
SEQ ID NO: 4027

Sb01g047980

Sorghum

Polynucleotide
SEQ ID NO: 1247

bicolor

Polypeptide
SEQ ID NO: 1248

Genomic
SEQ ID NO: 4028

Sb01g048040

Sorghum

Polynucleotide
SEQ ID NO: 1249

bicolor

Polypeptide
SEQ ID NO: 1250

Genomic
SEQ ID NO: 4029

Sb01g048280

Sorghum

Polynucleotide
SEQ ID NO: 1251

bicolor

Polypeptide
SEQ ID NO: 1252

Genomic
SEQ ID NO: 4030

Sb01g048590

Sorghum

Polynucleotide
SEQ ID NO: 1253

bicolor

Polypeptide
SEQ ID NO: 1254

Genomic
SEQ ID NO: 4031

Sb01g048810

Sorghum

Polynucleotide
SEQ ID NO: 1255

bicolor

Polypeptide
SEQ ID NO: 1256

Genomic
SEQ ID NO: 4032

Sb01g049190

Sorghum

Polynucleotide
SEQ ID NO: 1257

bicolor

Polypeptide
SEQ ID NO: 1258

Genomic
SEQ ID NO: 4033

Sb01g049210

Sorghum

Polynucleotide
SEQ ID NO: 1259

bicolor

Polypeptide
SEQ ID NO: 1260

Genomic
SEQ ID NO: 4034

Sb01g154600

Sorghum

Polynucleotide
SEQ ID NO: 1261

bicolor

Polypeptide
SEQ ID NO: 1262

Genomic
SEQ ID NO: 4035

Sb01g049970

Sorghum

Polynucleotide
SEQ ID NO: 1263

bicolor

Polypeptide
SEQ ID NO: 1264

Genomic
SEQ ID NO: 4036

Sb01g050070

Sorghum

Polynucleotide
SEQ ID NO: 1265

bicolor

Polypeptide
SEQ ID NO: 1266

Genomic
SEQ ID NO: 4037

Sb01g050190

Sorghum

Polynucleotide
SEQ ID NO: 1267

bicolor

Polypeptide
SEQ ID NO: 1268

Genomic
SEQ ID NO: 4038

Sb01g050670

Sorghum

Polynucleotide
SEQ ID NO: 1269

bicolor

Polypeptide
SEQ ID NO: 1270

Genomic
SEQ ID NO: 4039

Sb01g050680

Sorghum

Polynucleotide
SEQ ID NO: 1271

bicolor

Polypeptide
SEQ ID NO: 1272

Genomic
SEQ ID NO: 4040

Sb0224s002010

Sorghum

Polynucleotide
SEQ ID NO: 1273

bicolor

Polypeptide
SEQ ID NO: 1274

Genomic
SEQ ID NO: 4041

Sb02g000230

Sorghum

Polynucleotide
SEQ ID NO: 1275

bicolor

Polypeptide
SEQ ID NO: 1276

Genomic
SEQ ID NO: 4042

Sb02g000280

Sorghum

Polynucleotide
SEQ ID NO: 1277

bicolor

Polypeptide
SEQ ID NO: 1278

Genomic
SEQ ID NO: 4043

Sb02g000370

Sorghum

Polynucleotide
SEQ ID NO: 1279

bicolor

Polypeptide
SEQ ID NO: 1280

Genomic
SEQ ID NO: 4044

Sb02g000380

Sorghum

Polynucleotide
SEQ ID NO: 1281

bicolor

Polypeptide
SEQ ID NO: 1282

Genomic
SEQ ID NO: 4045

Sb02g000620

Sorghum

Polynucleotide
SEQ ID NO: 1283

bicolor

Polypeptide
SEQ ID NO: 1284

Genomic
SEQ ID NO: 4046

Sb02g000830

Sorghum

Polynucleotide
SEQ ID NO: 1285

bicolor

Polypeptide
SEQ ID NO: 1286

Genomic
SEQ ID NO: 4047

Sb02g001300

Sorghum

Polynucleotide
SEQ ID NO: 1287

bicolor

Polypeptide
SEQ ID NO: 1288

Genomic
SEQ ID NO: 4048

Sb01g000443

Sorghum

Polynucleotide
SEQ ID NO: 1289

bicolor

Polypeptide
SEQ ID NO: 1290

Genomic
SEQ ID NO: 4049

Sb02g001658

Sorghum

Polynucleotide
SEQ ID NO: 1291

bicolor

Polypeptide
SEQ ID NO: 1292

Genomic
SEQ ID NO: 4050

Sb02g002200

Sorghum

Polynucleotide
SEQ ID NO: 1293

bicolor

Polypeptide
SEQ ID NO: 1294

Genomic
SEQ ID NO: 4051

Sb02g002600

Sorghum

Polynucleotide
SEQ ID NO: 1295

bicolor

Polypeptide
SEQ ID NO: 1296

Genomic
SEQ ID NO: 4052

Sb02g002750

Sorghum

Polynucleotide
SEQ ID NO: 1297

bicolor

Polypeptide
SEQ ID NO: 1298

Genomic
SEQ ID NO: 4053

Sb02g003260

Sorghum

Polynucleotide
SEQ ID NO: 1299

bicolor

Polypeptide
SEQ ID NO: 1300

Genomic
SEQ ID NO: 4054

Sb02g003400

Sorghum

Polynucleotide
SEQ ID NO: 1301

bicolor

Polypeptide
SEQ ID NO: 1302

Genomic
SEQ ID NO: 4055

Sb02g003440

Sorghum

Polynucleotide
SEQ ID NO: 1303

bicolor

Polypeptide
SEQ ID NO: 1304

Genomic
SEQ ID NO: 4056

Sb02g003450

Sorghum

Polynucleotide
SEQ ID NO: 1305

bicolor

Polypeptide
SEQ ID NO: 1306

Genomic
SEQ ID NO: 4057

Sb02g003710

Sorghum

Polynucleotide
SEQ ID NO: 1307

bicolor

Polypeptide
SEQ ID NO: 1308

Genomic
SEQ ID NO: 4058

Sb02g004830

Sorghum

Polynucleotide
SEQ ID NO: 1309

bicolor

Polypeptide
SEQ ID NO: 1310

Genomic
SEQ ID NO: 4059

Sb02g005160

Sorghum

Polynucleotide
SEQ ID NO: 1311

bicolor

Polypeptide
SEQ ID NO: 1312

Genomic
SEQ ID NO: 4060

Sb02g005950

Sorghum

Polynucleotide
SEQ ID NO: 1313

bicolor

Polypeptide
SEQ ID NO: 1314

Genomic
SEQ ID NO: 4061

Sb02g006000

Sorghum

Polynucleotide
SEQ ID NO: 1315

bicolor

Polypeptide
SEQ ID NO: 1316

Genomic
SEQ ID NO: 4062

Sb02g006130

Sorghum

Polynucleotide
SEQ ID NO: 1317

bicolor

Polypeptide
SEQ ID NO: 1318

Genomic
SEQ ID NO: 4063

Sb02g006450

Sorghum

Polynucleotide
SEQ ID NO: 1319

bicolor

Polypeptide
SEQ ID NO: 1320

Genomic
SEQ ID NO: 4064

Sb02g006570

Sorghum

Polynucleotide
SEQ ID NO: 1321

bicolor

Polypeptide
SEQ ID NO: 1322

Genomic
SEQ ID NO: 4065

Sb02g015780

Sorghum

Polynucleotide
SEQ ID NO: 1323

bicolor

Polypeptide
SEQ ID NO: 1324

Genomic
SEQ ID NO: 4066

Sb02g007100

Sorghum

Polynucleotide
SEQ ID NO: 1325

bicolor

Polypeptide
SEQ ID NO: 1326

Genomic
SEQ ID NO: 4067

Sb02g007310

Sorghum

Polynucleotide
SEQ ID NO: 1327

bicolor

Polypeptide
SEQ ID NO: 1328

Genomic
SEQ ID NO: 4068

Sb02g007390

Sorghum

Polynucleotide
SEQ ID NO: 1329

bicolor

Polypeptide
SEQ ID NO: 1330

Genomic
SEQ ID NO: 4069

Sb02g007660

Sorghum

Polynucleotide
SEQ ID NO: 1331

bicolor

Polypeptide
SEQ ID NO: 1332

Genomic
SEQ ID NO: 4070

Sb02g007780

Sorghum

Polynucleotide
SEQ ID NO: 1333

bicolor

Polypeptide
SEQ ID NO: 1334

Genomic
SEQ ID NO: 4071

Sb02g007850

Sorghum

Polynucleotide
SEQ ID NO: 1335

bicolor

Polypeptide
SEQ ID NO: 1336

Genomic
SEQ ID NO: 4072

Sb02g019130

Sorghum

Polynucleotide
SEQ ID NO: 1337

bicolor

Polypeptide
SEQ ID NO: 1338

Genomic
SEQ ID NO: 4073

Sb02g007960

Sorghum

Polynucleotide
SEQ ID NO: 1339

bicolor

Polypeptide
SEQ ID NO: 1340

Genomic
SEQ ID NO: 4074

Sb02g008650

Sorghum

Polynucleotide
SEQ ID NO: 1341

bicolor

Polypeptide
SEQ ID NO: 1342

Genomic
SEQ ID NO: 4075

Sb02g008810

Sorghum

Polynucleotide
SEQ ID NO: 1343

bicolor

Polypeptide
SEQ ID NO: 1344

Genomic
SEQ ID NO: 4076

Sb02g008970

Sorghum

Polynucleotide
SEQ ID NO: 1345

bicolor

Polypeptide
SEQ ID NO: 1346

Genomic
SEQ ID NO: 4077

Sb02g009180

Sorghum

Polynucleotide
SEQ ID NO: 1347

bicolor

Polypeptide
SEQ ID NO: 1348

Genomic
SEQ ID NO: 4078

Sb02g009290

Sorghum

Polynucleotide
SEQ ID NO: 1349

bicolor

Polypeptide
SEQ ID NO: 1350

Genomic
SEQ ID NO: 4079

Sb02g009300

Sorghum

Polynucleotide
SEQ ID NO: 1351

bicolor

Polypeptide
SEQ ID NO: 1352

Genomic
SEQ ID NO: 4080

Sb02g009380

Sorghum

Polynucleotide
SEQ ID NO: 1353

bicolor

Polypeptide
SEQ ID NO: 1354

Genomic
SEQ ID NO: 4081

Sb02g009500

Sorghum

Polynucleotide
SEQ ID NO: 1355

bicolor

Polypeptide
SEQ ID NO: 1356

Genomic
SEQ ID NO: 4082

Sb02g009610

Sorghum

Polynucleotide
SEQ ID NO: 1357

bicolor

Polypeptide
SEQ ID NO: 1358

Genomic
SEQ ID NO: 4083

Sb02g009670

Sorghum

Polynucleotide
SEQ ID NO: 1359

bicolor

Polypeptide
SEQ ID NO: 1360

Genomic
SEQ ID NO: 4084

Sb02g009690

Sorghum

Polynucleotide
SEQ ID NO: 1361

bicolor

Polypeptide
SEQ ID NO: 1362

Genomic
SEQ ID NO: 4085

Sb02g009870

Sorghum

Polynucleotide
SEQ ID NO: 1363

bicolor

Polypeptide
SEQ ID NO: 1364

Genomic
SEQ ID NO: 4086

Sb02g010190

Sorghum

Polynucleotide
SEQ ID NO: 1365

bicolor

Polypeptide
SEQ ID NO: 1366

Genomic
SEQ ID NO: 4087

Sb02g043450

Sorghum

Polynucleotide
SEQ ID NO: 1367

bicolor

Polypeptide
SEQ ID NO: 1368

Genomic
SEQ ID NO: 4088

Sb02g011390

Sorghum

Polynucleotide
SEQ ID NO: 1369

bicolor

Polypeptide
SEQ ID NO: 1370

Genomic
SEQ ID NO: 4089

Sb02g060390

Sorghum

Polynucleotide
SEQ ID NO: 1371

bicolor

Polypeptide
SEQ ID NO: 1372

Genomic
SEQ ID NO: 4090

Sb02g018530

Sorghum

Polynucleotide
SEQ ID NO: 1373

bicolor

Polypeptide
SEQ ID NO: 1374

Genomic
SEQ ID NO: 4091

Sb02g112300

Sorghum

Polynucleotide
SEQ ID NO: 1375

bicolor

Polypeptide
SEQ ID NO: 1376

Genomic
SEQ ID NO: 4092

Sb02g021040

Sorghum

Polynucleotide
SEQ ID NO: 1377

bicolor

Polypeptide
SEQ ID NO: 1378

Genomic
SEQ ID NO: 4093

Sb02g021133

Sorghum

Polynucleotide
SEQ ID NO: 1379

bicolor

Polypeptide
SEQ ID NO: 1380

Genomic
SEQ ID NO: 4094

Sb02g021450

Sorghum

Polynucleotide
SEQ ID NO: 1381

bicolor

Polypeptide
SEQ ID NO: 1382

Genomic
SEQ ID NO: 4095

Sb02g133410

Sorghum

Polynucleotide
SEQ ID NO: 1383

bicolor

Polypeptide
SEQ ID NO: 1384

Genomic
SEQ ID NO: 4096

Sb02g021835

Sorghum

Polynucleotide
SEQ ID NO: 1385

bicolor

Polypeptide
SEQ ID NO: 1386

Genomic
SEQ ID NO: 4097

Sb02g022170

Sorghum

Polynucleotide
SEQ ID NO: 1387

bicolor

Polypeptide
SEQ ID NO: 1388

Genomic
SEQ ID NO: 4098

Sb02g022240

Sorghum

Polynucleotide
SEQ ID NO: 1389

bicolor

Polypeptide
SEQ ID NO: 1390

Genomic
SEQ ID NO: 4099

Sb02g022480

Sorghum

Polynucleotide
SEQ ID NO: 1391

bicolor

Polypeptide
SEQ ID NO: 1392

Genomic
SEQ ID NO: 4100

Sb02g022640

Sorghum

Polynucleotide
SEQ ID NO: 1393

bicolor

Polypeptide
SEQ ID NO: 1394

Genomic
SEQ ID NO: 4101

Sb02g022650

Sorghum

Polynucleotide
SEQ ID NO: 1395

bicolor

Polypeptide
SEQ ID NO: 1396

Genomic
SEQ ID NO: 4102

Sb02g022910

Sorghum

Polynucleotide
SEQ ID NO: 1397

bicolor

Polypeptide
SEQ ID NO: 1398

Genomic
SEQ ID NO: 4103

Sb02g022920

Sorghum

Polynucleotide
SEQ ID NO: 1399

bicolor

Polypeptide
SEQ ID NO: 1400

Genomic
SEQ ID NO: 4104

Sb02g022970

Sorghum

Polynucleotide
SEQ ID NO: 1401

bicolor

Polypeptide
SEQ ID NO: 1402

Genomic
SEQ ID NO: 4105

Sb02g023080

Sorghum

Polynucleotide
SEQ ID NO: 1403

bicolor

Polypeptide
SEQ ID NO: 1404

Genomic
SEQ ID NO: 4106

Sb02g023140

Sorghum

Polynucleotide
SEQ ID NO: 1405

bicolor

Polypeptide
SEQ ID NO: 1406

Genomic
SEQ ID NO: 4107

Sb02g023170

Sorghum

Polynucleotide
SEQ ID NO: 1407

bicolor

Polypeptide
SEQ ID NO: 1408

Genomic
SEQ ID NO: 4108

Sb02g023290

Sorghum

Polynucleotide
SEQ ID NO: 1409

bicolor

Polypeptide
SEQ ID NO: 1410

Genomic
SEQ ID NO: 4109

Sb02g023360

Sorghum

Polynucleotide
SEQ ID NO: 1411

bicolor

Polypeptide
SEQ ID NO: 1412

Genomic
SEQ ID NO: 4110

Sb02g023400

Sorghum

Polynucleotide
SEQ ID NO: 1413

bicolor

Polypeptide
SEQ ID NO: 1414

Genomic
SEQ ID NO: 4111

Sb02g023720

Sorghum

Polynucleotide
SEQ ID NO: 1415

bicolor

Polypeptide
SEQ ID NO: 1416

Genomic
SEQ ID NO: 4112

Sb02g023830

Sorghum

Polynucleotide
SEQ ID NO: 1417

bicolor

Polypeptide
SEQ ID NO: 1418

Genomic
SEQ ID NO: 4113

Sb02g024020

Sorghum

Polynucleotide
SEQ ID NO: 1419

bicolor

Polypeptide
SEQ ID NO: 1420

Genomic
SEQ ID NO: 4114

Sb02g024060

Sorghum

Polynucleotide
SEQ ID NO: 1421

bicolor

Polypeptide
SEQ ID NO: 1422

Genomic
SEQ ID NO: 4115

Sb02g024350

Sorghum

Polynucleotide
SEQ ID NO: 1423

bicolor

Polypeptide
SEQ ID NO: 1424

Genomic
SEQ ID NO: 4116

Sb02g024450

Sorghum

Polynucleotide
SEQ ID NO: 1425

bicolor

Polypeptide
SEQ ID NO: 1426

Genomic
SEQ ID NO: 4117

Sb02g024480

Sorghum

Polynucleotide
SEQ ID NO: 1427

bicolor

Polypeptide
SEQ ID NO: 1428

Genomic
SEQ ID NO: 4118

Sb02g024810

Sorghum

Polynucleotide
SEQ ID NO: 1429

bicolor

Polypeptide
SEQ ID NO: 1430

Genomic
SEQ ID NO: 4119

Sb02g024900

Sorghum

Polynucleotide
SEQ ID NO: 1431

bicolor

Polypeptide
SEQ ID NO: 1432

Genomic
SEQ ID NO: 4120

Sb02g025140

Sorghum

Polynucleotide
SEQ ID NO: 1433

bicolor

Polypeptide
SEQ ID NO: 1434

Genomic
SEQ ID NO: 4121

Sb02g025340

Sorghum

Polynucleotide
SEQ ID NO: 1435

bicolor

Polypeptide
SEQ ID NO: 1436

Genomic
SEQ ID NO: 4122

Sb02g025510

Sorghum

Polynucleotide
SEQ ID NO: 1437

bicolor

Polypeptide
SEQ ID NO: 1438

Genomic
SEQ ID NO: 4123

Sb02g025590

Sorghum

Polynucleotide
SEQ ID NO: 1439

bicolor

Polypeptide
SEQ ID NO: 1440

Genomic
SEQ ID NO: 4124

Sb02g025790

Sorghum

Polynucleotide
SEQ ID NO: 1441

bicolor

Polypeptide
SEQ ID NO: 1442

Genomic
SEQ ID NO: 4125

Sb02g026140

Sorghum

Polynucleotide
SEQ ID NO: 1443

bicolor

Polypeptide
SEQ ID NO: 1444

Genomic
SEQ ID NO: 4126

Sb02g026210

Sorghum

Polynucleotide
SEQ ID NO: 1445

bicolor

Polypeptide
SEQ ID NO: 1446

Genomic
SEQ ID NO: 4127

Sb02g026270

Sorghum

Polynucleotide
SEQ ID NO: 1447

bicolor

Polypeptide
SEQ ID NO: 1448

Genomic
SEQ ID NO: 4128

Sb02g026320

Sorghum

Polynucleotide
SEQ ID NO: 1449

bicolor

Polypeptide
SEQ ID NO: 1450

Genomic
SEQ ID NO: 4129

Sb02g026450

Sorghum

Polynucleotide
SEQ ID NO: 1451

bicolor

Polypeptide
SEQ ID NO: 1452

Genomic
SEQ ID NO: 4130

Sb02g026460

Sorghum

Polynucleotide
SEQ ID NO: 1453

bicolor

Polypeptide
SEQ ID NO: 1454

Genomic
SEQ ID NO: 4131

Sb02g026570

Sorghum

Polynucleotide
SEQ ID NO: 1455

bicolor

Polypeptide
SEQ ID NO: 1456

Genomic
SEQ ID NO: 4132

Sb02g026600

Sorghum

Polynucleotide
SEQ ID NO: 1457

bicolor

Polypeptide
SEQ ID NO: 1458

Genomic
SEQ ID NO: 4133

Sb02g026680

Sorghum

Polynucleotide
SEQ ID NO: 1459

bicolor

Polypeptide
SEQ ID NO: 1460

Genomic
SEQ ID NO: 4134

Sb10g008950

Sorghum

Polynucleotide
SEQ ID NO: 1461

bicolor

Polypeptide
SEQ ID NO: 1462

Genomic
SEQ ID NO: 4135

Sb02g026840

Sorghum

Polynucleotide
SEQ ID NO: 1463

bicolor

Polypeptide
SEQ ID NO: 1464

Genomic
SEQ ID NO: 4136

Sb02g027210

Sorghum

Polynucleotide
SEQ ID NO: 1465

bicolor

Polypeptide
SEQ ID NO: 1466

Genomic
SEQ ID NO: 4137

Sb02g027410

Sorghum

Polynucleotide
SEQ ID NO: 1467

bicolor

Polypeptide
SEQ ID NO: 1468

Genomic
SEQ ID NO: 4138

Sb02g027430

Sorghum

Polynucleotide
SEQ ID NO: 1469

bicolor

Polypeptide
SEQ ID NO: 1470

Genomic
SEQ ID NO: 4139

Sb02g153000

Sorghum

Polynucleotide
SEQ ID NO: 1471

bicolor

Polypeptide
SEQ ID NO: 1472

Genomic
SEQ ID NO: 4140

Sb02g153510

Sorghum

Polynucleotide
SEQ ID NO: 1473

bicolor

Polypeptide
SEQ ID NO: 1474

Genomic
SEQ ID NO: 4141

Sb02g028300

Sorghum

Polynucleotide
SEQ ID NO: 1475

bicolor

Polypeptide
SEQ ID NO: 1476

Genomic
SEQ ID NO: 4142

Sb02g028390

Sorghum

Polynucleotide
SEQ ID NO: 1477

bicolor

Polypeptide
SEQ ID NO: 1478

Genomic
SEQ ID NO: 4143

Sb02g028590

Sorghum

Polynucleotide
SEQ ID NO: 1479

bicolor

Polypeptide
SEQ ID NO: 1480

Genomic
SEQ ID NO: 4144

Sb02g028660

Sorghum

Polynucleotide
SEQ ID NO: 1481

bicolor

Polypeptide
SEQ ID NO: 1482

Genomic
SEQ ID NO: 4145

Sb02g028870

Sorghum

Polynucleotide
SEQ ID NO: 1483

bicolor

Polypeptide
SEQ ID NO: 1484

Genomic
SEQ ID NO: 4146

Sb02g028950

Sorghum

Polynucleotide
SEQ ID NO: 1485

bicolor

Polypeptide
SEQ ID NO: 1486

Genomic
SEQ ID NO: 4147

Sb02g029040

Sorghum

Polynucleotide
SEQ ID NO: 1487

bicolor

Polypeptide
SEQ ID NO: 1488

Genomic
SEQ ID NO: 4148

Sb02g029070

Sorghum

Polynucleotide
SEQ ID NO: 1489

bicolor

Polypeptide
SEQ ID NO: 1490

Genomic
SEQ ID NO: 4149

Sb02g029310

Sorghum

Polynucleotide
SEQ ID NO: 1491

bicolor

Polypeptide
SEQ ID NO: 1492

Genomic
SEQ ID NO: 4150

Sb02g029460

Sorghum

Polynucleotide
SEQ ID NO: 1493

bicolor

Polypeptide
SEQ ID NO: 1494

Genomic
SEQ ID NO: 4151

Sb02g029470

Sorghum

Polynucleotide
SEQ ID NO: 1495

bicolor

Polypeptide
SEQ ID NO: 1496

Genomic
SEQ ID NO: 4152

Sb02g029940

Sorghum

Polynucleotide
SEQ ID NO: 1497

bicolor

Polypeptide
SEQ ID NO: 1498

Genomic
SEQ ID NO: 4153

Sb02g156430

Sorghum

Polynucleotide
SEQ ID NO: 1499

bicolor

Polypeptide
SEQ ID NO: 1500

Genomic
SEQ ID NO: 4154

Sb02g030700

Sorghum

Polynucleotide
SEQ ID NO: 1501

bicolor

Polypeptide
SEQ ID NO: 1502

Genomic
SEQ ID NO: 4155

Sb02g030920

Sorghum

Polynucleotide
SEQ ID NO: 1503

bicolor

Polypeptide
SEQ ID NO: 1504

Genomic
SEQ ID NO: 4156

Sb02g031030

Sorghum

Polynucleotide
SEQ ID NO: 1505

bicolor

Polypeptide
SEQ ID NO: 1506

Genomic
SEQ ID NO: 4157

Sb02g031300

Sorghum

Polynucleotide
SEQ ID NO: 1507

bicolor

Polypeptide
SEQ ID NO: 1508

Genomic
SEQ ID NO: 4158

Sb02g031460

Sorghum

Polynucleotide
SEQ ID NO: 1509

bicolor

Polypeptide
SEQ ID NO: 1510

Genomic
SEQ ID NO: 4159

Sb02g031600

Sorghum

Polynucleotide
SEQ ID NO: 1511

bicolor

Polypeptide
SEQ ID NO: 1512

Genomic
SEQ ID NO: 4160

Sb02g032000

Sorghum

Polynucleotide
SEQ ID NO: 1513

bicolor

Polypeptide
SEQ ID NO: 1514

Genomic
SEQ ID NO: 4161

Sb02g032120

Sorghum

Polynucleotide
SEQ ID NO: 1515

bicolor

Polypeptide
SEQ ID NO: 1516

Genomic
SEQ ID NO: 4162

Sb02g032150

Sorghum

Polynucleotide
SEQ ID NO: 1517

bicolor

Polypeptide
SEQ ID NO: 1518

Genomic
SEQ ID NO: 4163

Sb02g032160

Sorghum

Polynucleotide
SEQ ID NO: 1519

bicolor

Polypeptide
SEQ ID NO: 1520

Genomic
SEQ ID NO: 4164

Sb02g032430

Sorghum

Polynucleotide
SEQ ID NO: 1521

bicolor

Polypeptide
SEQ ID NO: 1522

Genomic
SEQ ID NO: 4165

Sb02g032720

Sorghum

Polynucleotide
SEQ ID NO: 1523

bicolor

Polypeptide
SEQ ID NO: 1524

Genomic
SEQ ID NO: 4166

Sb02g032725

Sorghum

Polynucleotide
SEQ ID NO: 1525

bicolor

Polypeptide
SEQ ID NO: 1526

Genomic
SEQ ID NO: 4167

Sb02g033230

Sorghum

Polynucleotide
SEQ ID NO: 1527

bicolor

Polypeptide
SEQ ID NO: 1528

Genomic
SEQ ID NO: 4168

Sb02g033380

Sorghum

Polynucleotide
SEQ ID NO: 1529

bicolor

Polypeptide
SEQ ID NO: 1530

Genomic
SEQ ID NO: 4169

Sb02g033410

Sorghum

Polynucleotide
SEQ ID NO: 1531

bicolor

Polypeptide
SEQ ID NO: 1532

Genomic
SEQ ID NO: 4170

Sb02g033710

Sorghum

Polynucleotide
SEQ ID NO: 1533

bicolor

Polypeptide
SEQ ID NO: 1534

Genomic
SEQ ID NO: 4171

Sb02g033780

Sorghum

Polynucleotide
SEQ ID NO: 1535

bicolor

Polypeptide
SEQ ID NO: 1536

Genomic
SEQ ID NO: 4172

Sb02g034070

Sorghum

Polynucleotide
SEQ ID NO: 1537

bicolor

Polypeptide
SEQ ID NO: 1538

Genomic
SEQ ID NO: 4173

Sb02g034250

Sorghum

Polynucleotide
SEQ ID NO: 1539

bicolor

Polypeptide
SEQ ID NO: 1540

Genomic
SEQ ID NO: 4174

Sb02g034410

Sorghum

Polynucleotide
SEQ ID NO: 1541

bicolor

Polypeptide
SEQ ID NO: 1542

Genomic
SEQ ID NO: 4175

Sb02g034920

Sorghum

Polynucleotide
SEQ ID NO: 1543

bicolor

Polypeptide
SEQ ID NO: 1544

Genomic
SEQ ID NO: 4176

Sb02g035170

Sorghum

Polynucleotide
SEQ ID NO: 1545

bicolor

Polypeptide
SEQ ID NO: 1546

Genomic
SEQ ID NO: 4177

Sb02g164910

Sorghum

Polynucleotide
SEQ ID NO: 1547

bicolor

Polypeptide
SEQ ID NO: 1548

Genomic
SEQ ID NO: 4178

Sb02g035440

Sorghum

Polynucleotide
SEQ ID NO: 1549

bicolor

Polypeptide
SEQ ID NO: 1550

Genomic
SEQ ID NO: 4179

Sb02g035610

Sorghum

Polynucleotide
SEQ ID NO: 1551

bicolor

Polypeptide
SEQ ID NO: 1552

Genomic
SEQ ID NO: 4180

Sb02g036010

Sorghum

Polynucleotide
SEQ ID NO: 1553

bicolor

Polypeptide
SEQ ID NO: 1554

Genomic
SEQ ID NO: 4181

Sb02g036040

Sorghum

Polynucleotide
SEQ ID NO: 1555

bicolor

Polypeptide
SEQ ID NO: 1556

Genomic
SEQ ID NO: 4182

Sb02g036260

Sorghum

Polynucleotide
SEQ ID NO: 1557

bicolor

Polypeptide
SEQ ID NO: 1558

Genomic
SEQ ID NO: 4183

Sb02g036500

Sorghum

Polynucleotide
SEQ ID NO: 1559

bicolor

Polypeptide
SEQ ID NO: 1560

Genomic
SEQ ID NO: 4184

Sb02g036685

Sorghum

Polynucleotide
SEQ ID NO: 1561

bicolor

Polypeptide
SEQ ID NO: 1562

Genomic
SEQ ID NO: 4185

Sb02g036760

Sorghum

Polynucleotide
SEQ ID NO: 1563

bicolor

Polypeptide
SEQ ID NO: 1564

Genomic
SEQ ID NO: 4186

Sb02g036800

Sorghum

Polynucleotide
SEQ ID NO: 1565

bicolor

Polypeptide
SEQ ID NO: 1566

Genomic
SEQ ID NO: 4187

Sb02g037260

Sorghum

Polynucleotide
SEQ ID NO: 1567

bicolor

Polypeptide
SEQ ID NO: 1568

Genomic
SEQ ID NO: 4188

Sb02g037380

Sorghum

Polynucleotide
SEQ ID NO: 1569

bicolor

Polypeptide
SEQ ID NO: 1570

Genomic
SEQ ID NO: 4189

Sb02g037620

Sorghum

Polynucleotide
SEQ ID NO: 1571

bicolor

Polypeptide
SEQ ID NO: 1572

Genomic
SEQ ID NO: 4190

Sb02g037650

Sorghum

Polynucleotide
SEQ ID NO: 1573

bicolor

Polypeptide
SEQ ID NO: 1574

Genomic
SEQ ID NO: 4191

Sb02g037860

Sorghum

Polynucleotide
SEQ ID NO: 1575

bicolor

Polypeptide
SEQ ID NO: 1576

Genomic
SEQ ID NO: 4192

Sb02g037875

Sorghum

Polynucleotide
SEQ ID NO: 1577

bicolor

Polypeptide
SEQ ID NO: 1578

Genomic
SEQ ID NO: 4193

Sb02g038020

Sorghum

Polynucleotide
SEQ ID NO: 1579

bicolor

Polypeptide
SEQ ID NO: 1580

Genomic
SEQ ID NO: 4194

Sb02g169130

Sorghum

Polynucleotide
SEQ ID NO: 1581

bicolor

Polypeptide
SEQ ID NO: 1582

Genomic
SEQ ID NO: 4195

Sb02g038640

Sorghum

Polynucleotide
SEQ ID NO: 1583

bicolor

Polypeptide
SEQ ID NO: 1584

Genomic
SEQ ID NO: 4196

Sb02g038710

Sorghum

Polynucleotide
SEQ ID NO: 1585

bicolor

Polypeptide
SEQ ID NO: 1586

Genomic
SEQ ID NO: 4197

Sb02g039120

Sorghum

Polynucleotide
SEQ ID NO: 1587

bicolor

Polypeptide
SEQ ID NO: 1588

Genomic
SEQ ID NO: 4198

Sb02g039190

Sorghum

Polynucleotide
SEQ ID NO: 1589

bicolor

Polypeptide
SEQ ID NO: 1590

Genomic
SEQ ID NO: 4199

Sb02g170670

Sorghum

Polynucleotide
SEQ ID NO: 1591

bicolor

Polypeptide
SEQ ID NO: 1592

Genomic
SEQ ID NO: 4200

Sb02g039560

Sorghum

Polynucleotide
SEQ ID NO: 1593

bicolor

Polypeptide
SEQ ID NO: 1594

Genomic
SEQ ID NO: 4201

Sb02g041830

Sorghum

Polynucleotide
SEQ ID NO: 1595

bicolor

Polypeptide
SEQ ID NO: 1596

Genomic
SEQ ID NO: 4202

Sb02g039920

Sorghum

Polynucleotide
SEQ ID NO: 1597

bicolor

Polypeptide
SEQ ID NO: 1598

Genomic
SEQ ID NO: 4203

Sb02g040320

Sorghum

Polynucleotide
SEQ ID NO: 1599

bicolor

Polypeptide
SEQ ID NO: 1600

Genomic
SEQ ID NO: 4204

Sb02g040490

Sorghum

Polynucleotide
SEQ ID NO: 1601

bicolor

Polypeptide
SEQ ID NO: 1602

Genomic
SEQ ID NO: 4205

Sb02g040530

Sorghum

Polynucleotide
SEQ ID NO: 1603

bicolor

Polypeptide
SEQ ID NO: 1604

Genomic
SEQ ID NO: 4206

Sb02g040650

Sorghum

Polynucleotide
SEQ ID NO: 1605

bicolor

Polypeptide
SEQ ID NO: 1606

Genomic
SEQ ID NO: 4207

Sb02g041150

Sorghum

Polynucleotide
SEQ ID NO: 1607

bicolor

Polypeptide
SEQ ID NO: 1608

Genomic
SEQ ID NO: 4208

Sb02g041160

Sorghum

Polynucleotide
SEQ ID NO: 1609

bicolor

Polypeptide
SEQ ID NO: 1610

Genomic
SEQ ID NO: 4209

Sb02g041240

Sorghum

Polynucleotide
SEQ ID NO: 1611

bicolor

Polypeptide
SEQ ID NO: 1612

Genomic
SEQ ID NO: 4210

Sb02g041360

Sorghum

Polynucleotide
SEQ ID NO: 1613

bicolor

Polypeptide
SEQ ID NO: 1614

Genomic
SEQ ID NO: 4211

Sb02g042210

Sorghum

Polynucleotide
SEQ ID NO: 1615

bicolor

Polypeptide
SEQ ID NO: 1616

Genomic
SEQ ID NO: 4212

Sb02g042230

Sorghum

Polynucleotide
SEQ ID NO: 1617

bicolor

Polypeptide
SEQ ID NO: 1618

Genomic
SEQ ID NO: 4213

Sb02g042260

Sorghum

Polynucleotide
SEQ ID NO: 1619

bicolor

Polypeptide
SEQ ID NO: 1620

Genomic
SEQ ID NO: 4214

Sb02g042750

Sorghum

Polynucleotide
SEQ ID NO: 1621

bicolor

Polypeptide
SEQ ID NO: 1622

Genomic
SEQ ID NO: 4215

Sb02g042880

Sorghum

Polynucleotide
SEQ ID NO: 1623

bicolor

Polypeptide
SEQ ID NO: 1624

Genomic
SEQ ID NO: 4216

Sb02g042960

Sorghum

Polynucleotide
SEQ ID NO: 1625

bicolor

Polypeptide
SEQ ID NO: 1626

Genomic
SEQ ID NO: 4217

Sb02g043020

Sorghum

Polynucleotide
SEQ ID NO: 1627

bicolor

Polypeptide
SEQ ID NO: 1628

Genomic
SEQ ID NO: 4218

Sb02g043310

Sorghum

Polynucleotide
SEQ ID NO: 1629

bicolor

Polypeptide
SEQ ID NO: 1630

Genomic
SEQ ID NO: 4219

Sb02g043400

Sorghum

Polynucleotide
SEQ ID NO: 1631

bicolor

Polypeptide
SEQ ID NO: 1632

Genomic
SEQ ID NO: 4220

Sb02g043440

Sorghum

Polynucleotide
SEQ ID NO: 1633

bicolor

Polypeptide
SEQ ID NO: 1634

Genomic
SEQ ID NO: 4221

Sb02g176750

Sorghum

Polynucleotide
SEQ ID NO: 1635

bicolor

Polypeptide
SEQ ID NO: 1636

Genomic
SEQ ID NO: 4222

Sb03g000370

Sorghum

Polynucleotide
SEQ ID NO: 1637

bicolor

Polypeptide
SEQ ID NO: 1638

Genomic
SEQ ID NO: 4223

Sb03g000670

Sorghum

Polynucleotide
SEQ ID NO: 1639

bicolor

Polypeptide
SEQ ID NO: 1640

Genomic
SEQ ID NO: 4224

Sb03g000690

Sorghum

Polynucleotide
SEQ ID NO: 1641

bicolor

Polypeptide
SEQ ID NO: 1642

Genomic
SEQ ID NO: 4225

Sb03g000850

Sorghum

Polynucleotide
SEQ ID NO: 1643

bicolor

Polypeptide
SEQ ID NO: 1644

Genomic
SEQ ID NO: 4226

Sb03g000930

Sorghum

Polynucleotide
SEQ ID NO: 1645

bicolor

Polypeptide
SEQ ID NO: 1646

Genomic
SEQ ID NO: 4227

Sb03g001020

Sorghum

Polynucleotide
SEQ ID NO: 1647

bicolor

Polypeptide
SEQ ID NO: 1648

Genomic
SEQ ID NO: 4228

Sb03g001140

Sorghum

Polynucleotide
SEQ ID NO: 1649

bicolor

Polypeptide
SEQ ID NO: 1650

Genomic
SEQ ID NO: 4229

Sb03g004100

Sorghum

Polynucleotide
SEQ ID NO: 1651

bicolor

Polypeptide
SEQ ID NO: 1652

Genomic
SEQ ID NO: 4230

Sb03g001430

Sorghum

Polynucleotide
SEQ ID NO: 1653

bicolor

Polypeptide
SEQ ID NO: 1654

Genomic
SEQ ID NO: 4231

Sb03g001440

Sorghum

Polynucleotide
SEQ ID NO: 1655

bicolor

Polypeptide
SEQ ID NO: 1656

Genomic
SEQ ID NO: 4232

Sb03g001590

Sorghum

Polynucleotide
SEQ ID NO: 1657

bicolor

Polypeptide
SEQ ID NO: 1658

Genomic
SEQ ID NO: 4233

Sb03g001800

Sorghum

Polynucleotide
SEQ ID NO: 1659

bicolor

Polypeptide
SEQ ID NO: 1660

Genomic
SEQ ID NO: 4234

Sb03g001990

Sorghum

Polynucleotide
SEQ ID NO: 1661

bicolor

Polypeptide
SEQ ID NO: 1662

Genomic
SEQ ID NO: 4235

Sb03g002660

Sorghum

Polynucleotide
SEQ ID NO: 1663

bicolor

Polypeptide
SEQ ID NO: 1664

Genomic
SEQ ID NO: 4236

Sb03g002990

Sorghum

Polynucleotide
SEQ ID NO: 1665

bicolor

Polypeptide
SEQ ID NO: 1666

Genomic
SEQ ID NO: 4237

Sb03g003063

Sorghum

Polynucleotide
SEQ ID NO: 1667

bicolor

Polypeptide
SEQ ID NO: 1668

Genomic
SEQ ID NO: 4238

Sb03g003130

Sorghum

Polynucleotide
SEQ ID NO: 1669

bicolor

Polypeptide
SEQ ID NO: 1670

Genomic
SEQ ID NO: 4239

Sb03g003700

Sorghum

Polynucleotide
SEQ ID NO: 1671

bicolor

Polypeptide
SEQ ID NO: 1672

Genomic
SEQ ID NO: 4240

Sb03g004110

Sorghum

Polynucleotide
SEQ ID NO: 1673

bicolor

Polypeptide
SEQ ID NO: 1674

Genomic
SEQ ID NO: 4241

Sb03g004330

Sorghum

Polynucleotide
SEQ ID NO: 1675

bicolor

Polypeptide
SEQ ID NO: 1676

Genomic
SEQ ID NO: 4242

Sb03g004390

Sorghum

Polynucleotide
SEQ ID NO: 1677

bicolor

Polypeptide
SEQ ID NO: 1678

Genomic
SEQ ID NO: 4243

Sb03g004410

Sorghum

Polynucleotide
SEQ ID NO: 1679

bicolor

Polypeptide
SEQ ID NO: 1680

Genomic
SEQ ID NO: 4244

Sb03g004630

Sorghum

Polynucleotide
SEQ ID NO: 1681

bicolor

Polypeptide
SEQ ID NO: 1682

Genomic
SEQ ID NO: 4245

Sb03g004760

Sorghum

Polynucleotide
SEQ ID NO: 1683

bicolor

Polypeptide
SEQ ID NO: 1684

Genomic
SEQ ID NO: 4246

Sb03g004920

Sorghum

Polynucleotide
SEQ ID NO: 1685

bicolor

Polypeptide
SEQ ID NO: 1686

Genomic
SEQ ID NO: 4247

Sb03g005120

Sorghum

Polynucleotide
SEQ ID NO: 1687

bicolor

Polypeptide
SEQ ID NO: 1688

Genomic
SEQ ID NO: 4248

Sb03g005130

Sorghum

Polynucleotide
SEQ ID NO: 1689

bicolor

Polypeptide
SEQ ID NO: 1690

Genomic
SEQ ID NO: 4249

Sb03g005330

Sorghum

Polynucleotide
SEQ ID NO: 1691

bicolor

Polypeptide
SEQ ID NO: 1692

Genomic
SEQ ID NO: 4250

Sb03g005710

Sorghum

Polynucleotide
SEQ ID NO: 1693

bicolor

Polypeptide
SEQ ID NO: 1694

Genomic
SEQ ID NO: 4251

Sb03g005950

Sorghum

Polynucleotide
SEQ ID NO: 1695

bicolor

Polypeptide
SEQ ID NO: 1696

Genomic
SEQ ID NO: 4252

Sb03g006090

Sorghum

Polynucleotide
SEQ ID NO: 1697

bicolor

Polypeptide
SEQ ID NO: 1698

Genomic
SEQ ID NO: 4253

Sb03g014160

Sorghum

Polynucleotide
SEQ ID NO: 1699

bicolor

Polypeptide
SEQ ID NO: 1700

Genomic
SEQ ID NO: 4254

Sb03g007320

Sorghum

Polynucleotide
SEQ ID NO: 1701

bicolor

Polypeptide
SEQ ID NO: 1702

Genomic
SEQ ID NO: 4255

Sb03g008180

Sorghum

Polynucleotide
SEQ ID NO: 1703

bicolor

Polypeptide
SEQ ID NO: 1704

Genomic
SEQ ID NO: 4256

Sb03g008460

Sorghum

Polynucleotide
SEQ ID NO: 1705

bicolor

Polypeptide
SEQ ID NO: 1706

Genomic
SEQ ID NO: 4257

Sb03g017060

Sorghum

Polynucleotide
SEQ ID NO: 1707

bicolor

Polypeptide
SEQ ID NO: 1708

Genomic
SEQ ID NO: 4258

Sb03g008750

Sorghum

Polynucleotide
SEQ ID NO: 1709

bicolor

Polypeptide
SEQ ID NO: 1710

Genomic
SEQ ID NO: 4259

Sb03g009250

Sorghum

Polynucleotide
SEQ ID NO: 1711

bicolor

Polypeptide
SEQ ID NO: 1712

Genomic
SEQ ID NO: 4260

Sb03g009260

Sorghum

Polynucleotide
SEQ ID NO: 1713

bicolor

Polypeptide
SEQ ID NO: 1714

Genomic
SEQ ID NO: 4261

Sb03g009370

Sorghum

Polynucleotide
SEQ ID NO: 1715

bicolor

Polypeptide
SEQ ID NO: 1716

Genomic
SEQ ID NO: 4262

Sb03g018950

Sorghum

Polynucleotide
SEQ ID NO: 1717

bicolor

Polypeptide
SEQ ID NO: 1718

Genomic
SEQ ID NO: 4263

Sb03g009410

Sorghum

Polynucleotide
SEQ ID NO: 1719

bicolor

Polypeptide
SEQ ID NO: 1720

Genomic
SEQ ID NO: 4264

Sb03g009450

Sorghum

Polynucleotide
SEQ ID NO: 1721

bicolor

Polypeptide
SEQ ID NO: 1722

Genomic
SEQ ID NO: 4265

Sb03g009910

Sorghum

Polynucleotide
SEQ ID NO: 1723

bicolor

Polypeptide
SEQ ID NO: 1724

Genomic
SEQ ID NO: 4266

Sb03g010570

Sorghum

Polynucleotide
SEQ ID NO: 1725

bicolor

Polypeptide
SEQ ID NO: 1726

Genomic
SEQ ID NO: 4267

Sb03g010620

Sorghum

Polynucleotide
SEQ ID NO: 1727

bicolor

Polypeptide
SEQ ID NO: 1728

Genomic
SEQ ID NO: 4268

Sb03g010690

Sorghum

Polynucleotide
SEQ ID NO: 1729

bicolor

Polypeptide
SEQ ID NO: 1730

Genomic
SEQ ID NO: 4269

Sb03g010710

Sorghum

Polynucleotide
SEQ ID NO: 1731

bicolor

Polypeptide
SEQ ID NO: 1732

Genomic
SEQ ID NO: 4270

Sb07g025410

Sorghum

Polynucleotide
SEQ ID NO: 1733

bicolor

Polypeptide
SEQ ID NO: 1734

Genomic
SEQ ID NO: 4271

Sb03g010840

Sorghum

Polynucleotide
SEQ ID NO: 1735

bicolor

Polypeptide
SEQ ID NO: 1736

Genomic
SEQ ID NO: 4272

Sb03g010930

Sorghum

Polynucleotide
SEQ ID NO: 1737

bicolor

Polypeptide
SEQ ID NO: 1738

Genomic
SEQ ID NO: 4273

Sb03g010940

Sorghum

Polynucleotide
SEQ ID NO: 1739

bicolor

Polypeptide
SEQ ID NO: 1740

Genomic
SEQ ID NO: 4274

Sb03g011440

Sorghum

Polynucleotide
SEQ ID NO: 1741

bicolor

Polypeptide
SEQ ID NO: 1742

Genomic
SEQ ID NO: 4275

Sb03g011510

Sorghum

Polynucleotide
SEQ ID NO: 1743

bicolor

Polypeptide
SEQ ID NO: 1744

Genomic
SEQ ID NO: 4276

Sb03g024480

Sorghum

Polynucleotide
SEQ ID NO: 1745

bicolor

Polypeptide
SEQ ID NO: 1746

Genomic
SEQ ID NO: 4277

Sb03g011700

Sorghum

Polynucleotide
SEQ ID NO: 1747

bicolor

Polypeptide
SEQ ID NO: 1748

Genomic
SEQ ID NO: 4278

Sb03g012020

Sorghum

Polynucleotide
SEQ ID NO: 1749

bicolor

Polypeptide
SEQ ID NO: 1750

Genomic
SEQ ID NO: 4279

Sb03g012330

Sorghum

Polynucleotide
SEQ ID NO: 1751

bicolor

Polypeptide
SEQ ID NO: 1752

Genomic
SEQ ID NO: 4280

Sb03g013000

Sorghum

Polynucleotide
SEQ ID NO: 1753

bicolor

Polypeptide
SEQ ID NO: 1754

Genomic
SEQ ID NO: 4281

Sb03g013080

Sorghum

Polynucleotide
SEQ ID NO: 1755

bicolor

Polypeptide
SEQ ID NO: 1756

Genomic
SEQ ID NO: 4282

Sb03g013090

Sorghum

Polynucleotide
SEQ ID NO: 1757

bicolor

Polypeptide
SEQ ID NO: 1758

Genomic
SEQ ID NO: 4283

Sb03g013170

Sorghum

Polynucleotide
SEQ ID NO: 1759

bicolor

Polypeptide
SEQ ID NO: 1760

Genomic
SEQ ID NO: 4284

Sb03g013340

Sorghum

Polynucleotide
SEQ ID NO: 1761

bicolor

Polypeptide
SEQ ID NO: 1762

Genomic
SEQ ID NO: 4285

Sb03g033220

Sorghum

Polynucleotide
SEQ ID NO: 1763

bicolor

Polypeptide
SEQ ID NO: 1764

Genomic
SEQ ID NO: 4286

Sb03g013590

Sorghum

Polynucleotide
SEQ ID NO: 1765

bicolor

Polypeptide
SEQ ID NO: 1766

Genomic
SEQ ID NO: 4287

Sb03g013615

Sorghum

Polynucleotide
SEQ ID NO: 1767

bicolor

Polypeptide
SEQ ID NO: 1768

Genomic
SEQ ID NO: 4288

Sb03g013840

Sorghum

Polynucleotide
SEQ ID NO: 1769

bicolor

Polypeptide
SEQ ID NO: 1770

Genomic
SEQ ID NO: 4289

Sb03g014460

Sorghum

Polynucleotide
SEQ ID NO: 1771

bicolor

Polypeptide
SEQ ID NO: 1772

Genomic
SEQ ID NO: 4290

Sb03g014690

Sorghum

Polynucleotide
SEQ ID NO: 1773

bicolor

Polypeptide
SEQ ID NO: 1774

Genomic
SEQ ID NO: 4291

Sb03g014740

Sorghum

Polynucleotide
SEQ ID NO: 1775

bicolor

Polypeptide
SEQ ID NO: 1776

Genomic
SEQ ID NO: 4292

Sb03g016720

Sorghum

Polynucleotide
SEQ ID NO: 1777

bicolor

Polypeptide
SEQ ID NO: 1778

Genomic
SEQ ID NO: 4293

Sb03g095130

Sorghum

Polynucleotide
SEQ ID NO: 1779

bicolor

Polypeptide
SEQ ID NO: 1780

Genomic
SEQ ID NO: 4294

Sb03g021050

Sorghum

Polynucleotide
SEQ ID NO: 1781

bicolor

Polypeptide
SEQ ID NO: 1782

Genomic
SEQ ID NO: 4295

Sb03g022880

Sorghum

Polynucleotide
SEQ ID NO: 1783

bicolor

Polypeptide
SEQ ID NO: 1784

Genomic
SEQ ID NO: 4296

Sb03g023490

Sorghum

Polynucleotide
SEQ ID NO: 1785

bicolor

Polypeptide
SEQ ID NO: 1786

Genomic
SEQ ID NO: 4297

Sb03g126290

Sorghum

Polynucleotide
SEQ ID NO: 1787

bicolor

Polypeptide
SEQ ID NO: 1788

Genomic
SEQ ID NO: 4298

Sb03g126310

Sorghum

Polynucleotide
SEQ ID NO: 1789

bicolor

Polypeptide
SEQ ID NO: 1790

Genomic
SEQ ID NO: 4299

Sb03g025100

Sorghum

Polynucleotide
SEQ ID NO: 1791

bicolor

Polypeptide
SEQ ID NO: 1792

Genomic
SEQ ID NO: 4300

Sb03g025560

Sorghum

Polynucleotide
SEQ ID NO: 1793

bicolor

Polypeptide
SEQ ID NO: 1794

Genomic
SEQ ID NO: 4301

Sb03g025750

Sorghum

Polynucleotide
SEQ ID NO: 1795

bicolor

Polypeptide
SEQ ID NO: 1796

Genomic
SEQ ID NO: 4302

Sb03g026670

Sorghum

Polynucleotide
SEQ ID NO: 1797

bicolor

Polypeptide
SEQ ID NO: 1798

Genomic
SEQ ID NO: 4303

Sb03g027246

Sorghum

Polynucleotide
SEQ ID NO: 1799

bicolor

Polypeptide
SEQ ID NO: 1800

Genomic
SEQ ID NO: 4304

Sb03g027405

Sorghum

Polynucleotide
SEQ ID NO: 1801

bicolor

Polypeptide
SEQ ID NO: 1802

Genomic
SEQ ID NO: 4305

Sb03g027470

Sorghum

Polynucleotide
SEQ ID NO: 1803

bicolor

Polypeptide
SEQ ID NO: 1804

Genomic
SEQ ID NO: 4306

Sb03g028040

Sorghum

Polynucleotide
SEQ ID NO: 1805

bicolor

Polypeptide
SEQ ID NO: 1806

Genomic
SEQ ID NO: 4307

Sb03g028070

Sorghum

Polynucleotide
SEQ ID NO: 1807

bicolor

Polypeptide
SEQ ID NO: 1808

Genomic
SEQ ID NO: 4308

Sb03g028140

Sorghum

Polynucleotide
SEQ ID NO: 1809

bicolor

Polypeptide
SEQ ID NO: 1810

Genomic
SEQ ID NO: 4309

Sb03g028300

Sorghum

Polynucleotide
SEQ ID NO: 1811

bicolor

Polypeptide
SEQ ID NO: 1812

Genomic
SEQ ID NO: 4310

Sb03g028330

Sorghum

Polynucleotide
SEQ ID NO: 1813

bicolor

Polypeptide
SEQ ID NO: 1814

Genomic
SEQ ID NO: 4311

Sb03g028420

Sorghum

Polynucleotide
SEQ ID NO: 1815

bicolor

Polypeptide
SEQ ID NO: 1816

Genomic
SEQ ID NO: 4312

Sb03g028600

Sorghum

Polynucleotide
SEQ ID NO: 1817

bicolor

Polypeptide
SEQ ID NO: 1818

Genomic
SEQ ID NO: 4313

Sb03g028850

Sorghum

Polynucleotide
SEQ ID NO: 1819

bicolor

Polypeptide
SEQ ID NO: 1820

Genomic
SEQ ID NO: 4314

Sb03g029030

Sorghum

Polynucleotide
SEQ ID NO: 1821

bicolor

Polypeptide
SEQ ID NO: 1822

Genomic
SEQ ID NO: 4315

Sb03g029170

Sorghum

Polynucleotide
SEQ ID NO: 1823

bicolor

Polypeptide
SEQ ID NO: 1824

Genomic
SEQ ID NO: 4316

Sb03g029360

Sorghum

Polynucleotide
SEQ ID NO: 1825

bicolor

Polypeptide
SEQ ID NO: 1826

Genomic
SEQ ID NO: 4317

Sb03g029430

Sorghum

Polynucleotide
SEQ ID NO: 1827

bicolor

Polypeptide
SEQ ID NO: 1828

Genomic
SEQ ID NO: 4318

Sb03g029490

Sorghum

Polynucleotide
SEQ ID NO: 1829

bicolor

Polypeptide
SEQ ID NO: 1830

Genomic
SEQ ID NO: 4319

Sb03g030090

Sorghum

Polynucleotide
SEQ ID NO: 1831

bicolor

Polypeptide
SEQ ID NO: 1832

Genomic
SEQ ID NO: 4320

Sb03g030450

Sorghum

Polynucleotide
SEQ ID NO: 1833

bicolor

Polypeptide
SEQ ID NO: 1834

Genomic
SEQ ID NO: 4321

Sb03g154350

Sorghum

Polynucleotide
SEQ ID NO: 1835

bicolor

Polypeptide
SEQ ID NO: 1836

Genomic
SEQ ID NO: 4322

Sb03g030720

Sorghum

Polynucleotide
SEQ ID NO: 1837

bicolor

Polypeptide
SEQ ID NO: 1838

Genomic
SEQ ID NO: 4323

Sb03g031310

Sorghum

Polynucleotide
SEQ ID NO: 1839

bicolor

Polypeptide
SEQ ID NO: 1840

Genomic
SEQ ID NO: 4324

Sb03g031780

Sorghum

Polynucleotide
SEQ ID NO: 1841

bicolor

Polypeptide
SEQ ID NO: 1842

Genomic
SEQ ID NO: 4325

Sb03g031930

Sorghum

Polynucleotide
SEQ ID NO: 1843

bicolor

Polypeptide
SEQ ID NO: 1844

Genomic
SEQ ID NO: 4326

Sb03g031940

Sorghum

Polynucleotide
SEQ ID NO: 1845

bicolor

Polypeptide
SEQ ID NO: 1846

Genomic
SEQ ID NO: 4327

Sb03g031990

Sorghum

Polynucleotide
SEQ ID NO: 1847

bicolor

Polypeptide
SEQ ID NO: 1848

Genomic
SEQ ID NO: 4328

Sb09g001966

Sorghum

Polynucleotide
SEQ ID NO: 1849

bicolor

Polypeptide
SEQ ID NO: 1850

Genomic
SEQ ID NO: 4329

Sb03g032220

Sorghum

Polynucleotide
SEQ ID NO: 1851

bicolor

Polypeptide
SEQ ID NO: 1852

Genomic
SEQ ID NO: 4330

Sb03g032235

Sorghum

Polynucleotide
SEQ ID NO: 1853

bicolor

Polypeptide
SEQ ID NO: 1854

Genomic
SEQ ID NO: 4331

Sb03g032460

Sorghum

Polynucleotide
SEQ ID NO: 1855

bicolor

Polypeptide
SEQ ID NO: 1856

Genomic
SEQ ID NO: 4332

Sb03g032580

Sorghum

Polynucleotide
SEQ ID NO: 1857

bicolor

Polypeptide
SEQ ID NO: 1858

Genomic
SEQ ID NO: 4333

Sb03g032710

Sorghum

Polynucleotide
SEQ ID NO: 1859

bicolor

Polypeptide
SEQ ID NO: 1860

Genomic
SEQ ID NO: 4334

Sb03g033080

Sorghum

Polynucleotide
SEQ ID NO: 1861

bicolor

Polypeptide
SEQ ID NO: 1862

Genomic
SEQ ID NO: 4335

Sb03g033220

Sorghum

Polynucleotide
SEQ ID NO: 1863

bicolor

Polypeptide
SEQ ID NO: 1864

Genomic
SEQ ID NO: 4336

Sb03g033340

Sorghum

Polynucleotide
SEQ ID NO: 1865

bicolor

Polypeptide
SEQ ID NO: 1866

Genomic
SEQ ID NO: 4337

Sb03g033390

Sorghum

Polynucleotide
SEQ ID NO: 1867

bicolor

Polypeptide
SEQ ID NO: 1868

Genomic
SEQ ID NO: 4338

Sb03g033480

Sorghum

Polynucleotide
SEQ ID NO: 1869

bicolor

Polypeptide
SEQ ID NO: 1870

Genomic
SEQ ID NO: 4339

Sb03g033540

Sorghum

Polynucleotide
SEQ ID NO: 1871

bicolor

Polypeptide
SEQ ID NO: 1872

Genomic
SEQ ID NO: 4340

Sb03g033710

Sorghum

Polynucleotide
SEQ ID NO: 1873

bicolor

Polypeptide
SEQ ID NO: 1874

Genomic
SEQ ID NO: 4341

Sb03g159610

Sorghum

Polynucleotide
SEQ ID NO: 1875

bicolor

Polypeptide
SEQ ID NO: 1876

Genomic
SEQ ID NO: 4342

Sb03g034110

Sorghum

Polynucleotide
SEQ ID NO: 1877

bicolor

Polypeptide
SEQ ID NO: 1878

Genomic
SEQ ID NO: 4343

Sb03g034250

Sorghum

Polynucleotide
SEQ ID NO: 1879

bicolor

Polypeptide
SEQ ID NO: 1880

Genomic
SEQ ID NO: 4344

Sb03g034500

Sorghum

Polynucleotide
SEQ ID NO: 1881

bicolor

Polypeptide
SEQ ID NO: 1882

Genomic
SEQ ID NO: 4345

Sb03g034530

Sorghum

Polynucleotide
SEQ ID NO: 1883

bicolor

Polypeptide
SEQ ID NO: 1884

Genomic
SEQ ID NO: 4346

Sb03g034680

Sorghum

Polynucleotide
SEQ ID NO: 1885

bicolor

Polypeptide
SEQ ID NO: 1886

Genomic
SEQ ID NO: 4347

Sb03g034750

Sorghum

Polynucleotide
SEQ ID NO: 1887

bicolor

Polypeptide
SEQ ID NO: 1888

Genomic
SEQ ID NO: 4348

Sb03g034830

Sorghum

Polynucleotide
SEQ ID NO: 1889

bicolor

Polypeptide
SEQ ID NO: 1890

Genomic
SEQ ID NO: 4349

Sb03g035060

Sorghum

Polynucleotide
SEQ ID NO: 1891

bicolor

Polypeptide
SEQ ID NO: 1892

Genomic
SEQ ID NO: 4350

Sb03g035070

Sorghum

Polynucleotide
SEQ ID NO: 1893

bicolor

Polypeptide
SEQ ID NO: 1894

Genomic
SEQ ID NO: 4351

Sb03g035090

Sorghum

Polynucleotide
SEQ ID NO: 1895

bicolor

Polypeptide
SEQ ID NO: 1896

Genomic
SEQ ID NO: 4352

Sb03g035480

Sorghum

Polynucleotide
SEQ ID NO: 1897

bicolor

Polypeptide
SEQ ID NO: 1898

Genomic
SEQ ID NO: 4353

Sb03g035650

Sorghum

Polynucleotide
SEQ ID NO: 1899

bicolor

Polypeptide
SEQ ID NO: 1900

Genomic
SEQ ID NO: 4354

Sb03g035750

Sorghum

Polynucleotide
SEQ ID NO: 1901

bicolor

Polypeptide
SEQ ID NO: 1902

Genomic
SEQ ID NO: 4355

Sb03g162110

Sorghum

Polynucleotide
SEQ ID NO: 1903

bicolor

Polypeptide
SEQ ID NO: 1904

Genomic
SEQ ID NO: 4356

Sb03g036255

Sorghum

Polynucleotide
SEQ ID NO: 1905

bicolor

Polypeptide
SEQ ID NO: 1906

Genomic
SEQ ID NO: 4357

Sb03g036390

Sorghum

Polynucleotide
SEQ ID NO: 1907

bicolor

Polypeptide
SEQ ID NO: 1908

Genomic
SEQ ID NO: 4358

Sb03g036610

Sorghum

Polynucleotide
SEQ ID NO: 1909

bicolor

Polypeptide
SEQ ID NO: 1910

Genomic
SEQ ID NO: 4359

Sb03g036780

Sorghum

Polynucleotide
SEQ ID NO: 1911

bicolor

Polypeptide
SEQ ID NO: 1912

Genomic
SEQ ID NO: 4360

Sb03g036810

Sorghum

Polynucleotide
SEQ ID NO: 1913

bicolor

Polypeptide
SEQ ID NO: 1914

Genomic
SEQ ID NO: 4361

Sb03g037040

Sorghum

Polynucleotide
SEQ ID NO: 1915

bicolor

Polypeptide
SEQ ID NO: 1916

Genomic
SEQ ID NO: 4362

Sb03g037200

Sorghum

Polynucleotide
SEQ ID NO: 1917

bicolor

Polypeptide
SEQ ID NO: 1918

Genomic
SEQ ID NO: 4363

Sb03g037490

Sorghum

Polynucleotide
SEQ ID NO: 1919

bicolor

Polypeptide
SEQ ID NO: 1920

Genomic
SEQ ID NO: 4364

Sb03g037590

Sorghum

Polynucleotide
SEQ ID NO: 1921

bicolor

Polypeptide
SEQ ID NO: 1922

Genomic
SEQ ID NO: 4365

Sb03g165210

Sorghum

Polynucleotide
SEQ ID NO: 1923

bicolor

Polypeptide
SEQ ID NO: 1924

Genomic
SEQ ID NO: 4366

Sb03g037900

Sorghum

Polynucleotide
SEQ ID NO: 1925

bicolor

Polypeptide
SEQ ID NO: 1926

Genomic
SEQ ID NO: 4367

Sb03g037920

Sorghum

Polynucleotide
SEQ ID NO: 1927

bicolor

Polypeptide
SEQ ID NO: 1928

Genomic
SEQ ID NO: 4368

Sb03g038020

Sorghum

Polynucleotide
SEQ ID NO: 1929

bicolor

Polypeptide
SEQ ID NO: 1930

Genomic
SEQ ID NO: 4369

Sb03g038110

Sorghum

Polynucleotide
SEQ ID NO: 1931

bicolor

Polypeptide
SEQ ID NO: 1932

Genomic
SEQ ID NO: 4370

Sb03g038330

Sorghum

Polynucleotide
SEQ ID NO: 1933

bicolor

Polypeptide
SEQ ID NO: 1934

Genomic
SEQ ID NO: 4371

Sb03g038410

Sorghum

Polynucleotide
SEQ ID NO: 1935

bicolor

Polypeptide
SEQ ID NO: 1936

Genomic
SEQ ID NO: 4372

Sb03g038680

Sorghum

Polynucleotide
SEQ ID NO: 1937

bicolor

Polypeptide
SEQ ID NO: 1938

Genomic
SEQ ID NO: 4373

Sb03g166730

Sorghum

Polynucleotide
SEQ ID NO: 1939

bicolor

Polypeptide
SEQ ID NO: 1940

Genomic
SEQ ID NO: 4374

Sb03g039170

Sorghum

Polynucleotide
SEQ ID NO: 1941

bicolor

Polypeptide
SEQ ID NO: 1942

Genomic
SEQ ID NO: 4375

Sb03g167060

Sorghum

Polynucleotide
SEQ ID NO: 1943

bicolor

Polypeptide
SEQ ID NO: 1944

Genomic
SEQ ID NO: 4376

Sb03g039440

Sorghum

Polynucleotide
SEQ ID NO: 1945

bicolor

Polypeptide
SEQ ID NO: 1946

Genomic
SEQ ID NO: 4377

Sb03g039430

Sorghum

Polynucleotide
SEQ ID NO: 1947

bicolor

Polypeptide
SEQ ID NO: 1948

Genomic
SEQ ID NO: 4378

Sb03g039480

Sorghum

Polynucleotide
SEQ ID NO: 1949

bicolor

Polypeptide
SEQ ID NO: 1950

Genomic
SEQ ID NO: 4379

Sb03g039670

Sorghum

Polynucleotide
SEQ ID NO: 1951

bicolor

Polypeptide
SEQ ID NO: 1952

Genomic
SEQ ID NO: 4380

Sb03g039740

Sorghum

Polynucleotide
SEQ ID NO: 1953

bicolor

Polypeptide
SEQ ID NO: 1954

Genomic
SEQ ID NO: 4381

Sb03g039900

Sorghum

Polynucleotide
SEQ ID NO: 1955

bicolor

Polypeptide
SEQ ID NO: 1956

Genomic
SEQ ID NO: 4382

Sb03g040240

Sorghum

Polynucleotide
SEQ ID NO: 1957

bicolor

Polypeptide
SEQ ID NO: 1958

Genomic
SEQ ID NO: 4383

Sb03g040530

Sorghum

Polynucleotide
SEQ ID NO: 1959

bicolor

Polypeptide
SEQ ID NO: 1960

Genomic
SEQ ID NO: 4384

Sb03g040720

Sorghum

Polynucleotide
SEQ ID NO: 1961

bicolor

Polypeptide
SEQ ID NO: 1962

Genomic
SEQ ID NO: 4385

Sb03g040830

Sorghum

Polynucleotide
SEQ ID NO: 1963

bicolor

Polypeptide
SEQ ID NO: 1964

Genomic
SEQ ID NO: 4386

Sb03g040840

Sorghum

Polynucleotide
SEQ ID NO: 1965

bicolor

Polypeptide
SEQ ID NO: 1966

Genomic
SEQ ID NO: 4387

Sb03g041040

Sorghum

Polynucleotide
SEQ ID NO: 1967

bicolor

Polypeptide
SEQ ID NO: 1968

Genomic
SEQ ID NO: 4388

Sb03g041330

Sorghum

Polynucleotide
SEQ ID NO: 1969

bicolor

Polypeptide
SEQ ID NO: 1970

Genomic
SEQ ID NO: 4389

Sb03g041430

Sorghum

Polynucleotide
SEQ ID NO: 1971

bicolor

Polypeptide
SEQ ID NO: 1972

Genomic
SEQ ID NO: 4390

Sb03g041560

Sorghum

Polynucleotide
SEQ ID NO: 1973

bicolor

Polypeptide
SEQ ID NO: 1974

Genomic
SEQ ID NO: 4391

Sb03g041770

Sorghum

Polynucleotide
SEQ ID NO: 1975

bicolor

Polypeptide
SEQ ID NO: 1976

Genomic
SEQ ID NO: 4392

Sb03g041910

Sorghum

Polynucleotide
SEQ ID NO: 1977

bicolor

Polypeptide
SEQ ID NO: 1978

Genomic
SEQ ID NO: 4393

Sb03g172070

Sorghum

Polynucleotide
SEQ ID NO: 1979

bicolor

Polypeptide
SEQ ID NO: 1980

Genomic
SEQ ID NO: 4394

Sb03g042960

Sorghum

Polynucleotide
SEQ ID NO: 1981

bicolor

Polypeptide
SEQ ID NO: 1982

Genomic
SEQ ID NO: 4395

Sb03g043040

Sorghum

Polynucleotide
SEQ ID NO: 1983

bicolor

Polypeptide
SEQ ID NO: 1984

Genomic
SEQ ID NO: 4396

Sb03g043420

Sorghum

Polynucleotide
SEQ ID NO: 1985

bicolor

Polypeptide
SEQ ID NO: 1986

Genomic
SEQ ID NO: 4397

Sb03g043430

Sorghum

Polynucleotide
SEQ ID NO: 1987

bicolor

Polypeptide
SEQ ID NO: 1988

Genomic
SEQ ID NO: 4398

Sb03g172850

Sorghum

Polynucleotide
SEQ ID NO: 1989

bicolor

Polypeptide
SEQ ID NO: 1990

Genomic
SEQ ID NO: 4399

Sb03g043690

Sorghum

Polynucleotide
SEQ ID NO: 1991

bicolor

Polypeptide
SEQ ID NO: 1992

Genomic
SEQ ID NO: 4400

Sb03g044130

Sorghum

Polynucleotide
SEQ ID NO: 1993

bicolor

Polypeptide
SEQ ID NO: 1994

Genomic
SEQ ID NO: 4401

Sb03g044160

Sorghum

Polynucleotide
SEQ ID NO: 1995

bicolor

Polypeptide
SEQ ID NO: 1996

Genomic
SEQ ID NO: 4402

Sb03g044200

Sorghum

Polynucleotide
SEQ ID NO: 1997

bicolor

Polypeptide
SEQ ID NO: 1998

Genomic
SEQ ID NO: 4403

Sb03g044240

Sorghum

Polynucleotide
SEQ ID NO: 1999

bicolor

Polypeptide
SEQ ID NO: 2000

Genomic
SEQ ID NO: 4404

Sb03g044420

Sorghum

Polynucleotide
SEQ ID NO: 2001

bicolor

Polypeptide
SEQ ID NO: 2002

Genomic
SEQ ID NO: 4405

Sb03g044530

Sorghum

Polynucleotide
SEQ ID NO: 2003

bicolor

Polypeptide
SEQ ID NO: 2004

Genomic
SEQ ID NO: 4406

Sb03g044580

Sorghum

Polynucleotide
SEQ ID NO: 2005

bicolor

Polypeptide
SEQ ID NO: 2006

Genomic
SEQ ID NO: 4407

Sb03g044630

Sorghum

Polynucleotide
SEQ ID NO: 2007

bicolor

Polypeptide
SEQ ID NO: 2008

Genomic
SEQ ID NO: 4408

Sb03g045290

Sorghum

Polynucleotide
SEQ ID NO: 2009

bicolor

Polypeptide
SEQ ID NO: 2010

Genomic
SEQ ID NO: 4409

Sb03g045340

Sorghum

Polynucleotide
SEQ ID NO: 2011

bicolor

Polypeptide
SEQ ID NO: 2012

Genomic
SEQ ID NO: 4410

Sb03g045390

Sorghum

Polynucleotide
SEQ ID NO: 2013

bicolor

Polypeptide
SEQ ID NO: 2014

Genomic
SEQ ID NO: 4411

Sb03g175730

Sorghum

Polynucleotide
SEQ ID NO: 2015

bicolor

Polypeptide
SEQ ID NO: 2016

Genomic
SEQ ID NO: 4412

Sb03g045990

Sorghum

Polynucleotide
SEQ ID NO: 2017

bicolor

Polypeptide
SEQ ID NO: 2018

Genomic
SEQ ID NO: 4413

Sb03g046080

Sorghum

Polynucleotide
SEQ ID NO: 2019

bicolor

Polypeptide
SEQ ID NO: 2020

Genomic
SEQ ID NO: 4414

Sb03g046660

Sorghum

Polynucleotide
SEQ ID NO: 2021

bicolor

Polypeptide
SEQ ID NO: 2022

Genomic
SEQ ID NO: 4415

Sb03g047230

Sorghum

Polynucleotide
SEQ ID NO: 2023

bicolor

Polypeptide
SEQ ID NO: 2024

Genomic
SEQ ID NO: 4416

Sb04g003200

Sorghum

Polynucleotide
SEQ ID NO: 2025

bicolor

Polypeptide
SEQ ID NO: 2026

Genomic
SEQ ID NO: 4417

Sb04g001190

Sorghum

Polynucleotide
SEQ ID NO: 2027

bicolor

Polypeptide
SEQ ID NO: 2028

Genomic
SEQ ID NO: 4418

Sb04g001270

Sorghum

Polynucleotide
SEQ ID NO: 2029

bicolor

Polypeptide
SEQ ID NO: 2030

Genomic
SEQ ID NO: 4419

Sb04g001550

Sorghum

Polynucleotide
SEQ ID NO: 2031

bicolor

Polypeptide
SEQ ID NO: 2032

Genomic
SEQ ID NO: 4420

Sb04g001620

Sorghum

Polynucleotide
SEQ ID NO: 2033

bicolor

Polypeptide
SEQ ID NO: 2034

Genomic
SEQ ID NO: 4421

Sb04g001730

Sorghum

Polynucleotide
SEQ ID NO: 2035

bicolor

Polypeptide
SEQ ID NO: 2036

Genomic
SEQ ID NO: 4422

Sb04g001810

Sorghum

Polynucleotide
SEQ ID NO: 2037

bicolor

Polypeptide
SEQ ID NO: 2038

Genomic
SEQ ID NO: 4423

Sb04g038150

Sorghum

Polynucleotide
SEQ ID NO: 2039

bicolor

Polypeptide
SEQ ID NO: 2040

Genomic
SEQ ID NO: 4424

Sb04g002080

Sorghum

Polynucleotide
SEQ ID NO: 2041

bicolor

Polypeptide
SEQ ID NO: 2042

Genomic
SEQ ID NO: 4425

Sb04g002450

Sorghum

Polynucleotide
SEQ ID NO: 2043

bicolor

Polypeptide
SEQ ID NO: 2044

Genomic
SEQ ID NO: 4426

Sb04g002790

Sorghum

Polynucleotide
SEQ ID NO: 2045

bicolor

Polypeptide
SEQ ID NO: 2046

Genomic
SEQ ID NO: 4427

Sb04g006650

Sorghum

Polynucleotide
SEQ ID NO: 2047

bicolor

Polypeptide
SEQ ID NO: 2048

Genomic
SEQ ID NO: 4428

Sb04g003440

Sorghum

Polynucleotide
SEQ ID NO: 2049

bicolor

Polypeptide
SEQ ID NO: 2050

Genomic
SEQ ID NO: 4429

Sb04g003780

Sorghum

Polynucleotide
SEQ ID NO: 2051

bicolor

Polypeptide
SEQ ID NO: 2052

Genomic
SEQ ID NO: 4430

Sb04g003970

Sorghum

Polynucleotide
SEQ ID NO: 2053

bicolor

Polypeptide
SEQ ID NO: 2054

Genomic
SEQ ID NO: 4431

Sb04g004670

Sorghum

Polynucleotide
SEQ ID NO: 2055

bicolor

Polypeptide
SEQ ID NO: 2056

Genomic
SEQ ID NO: 4432

Sb04g004830

Sorghum

Polynucleotide
SEQ ID NO: 2057

bicolor

Polypeptide
SEQ ID NO: 2058

Genomic
SEQ ID NO: 4433

Sb04g004850

Sorghum

Polynucleotide
SEQ ID NO: 2059

bicolor

Polypeptide
SEQ ID NO: 2060

Genomic
SEQ ID NO: 4434

Sb04g005150

Sorghum

Polynucleotide
SEQ ID NO: 2061

bicolor

Polypeptide
SEQ ID NO: 2062

Genomic
SEQ ID NO: 4435

Sb04g005630

Sorghum

Polynucleotide
SEQ ID NO: 2063

bicolor

Polypeptide
SEQ ID NO: 2064

Genomic
SEQ ID NO: 4436

Sb04g005680

Sorghum

Polynucleotide
SEQ ID NO: 2065

bicolor

Polypeptide
SEQ ID NO: 2066

Genomic
SEQ ID NO: 4437

Sb04g005710

Sorghum

Polynucleotide
SEQ ID NO: 2067

bicolor

Polypeptide
SEQ ID NO: 2068

Genomic
SEQ ID NO: 4438

Sb04g005810

Sorghum

Polynucleotide
SEQ ID NO: 2069

bicolor

Polypeptide
SEQ ID NO: 2070

Genomic
SEQ ID NO: 4439

Sb04g006010

Sorghum

Polynucleotide
SEQ ID NO: 2071

bicolor

Polypeptide
SEQ ID NO: 2072

Genomic
SEQ ID NO: 4440

Sb04g006370

Sorghum

Polynucleotide
SEQ ID NO: 2073

bicolor

Polypeptide
SEQ ID NO: 2074

Genomic
SEQ ID NO: 4441

Sb04g006440

Sorghum

Polynucleotide
SEQ ID NO: 2075

bicolor

Polypeptide
SEQ ID NO: 2076

Genomic
SEQ ID NO: 4442

Sb04g006450

Sorghum

Polynucleotide
SEQ ID NO: 2077

bicolor

Polypeptide
SEQ ID NO: 2078

Genomic
SEQ ID NO: 4443

Sb04g006710

Sorghum

Polynucleotide
SEQ ID NO: 2079

bicolor

Polypeptide
SEQ ID NO: 2080

Genomic
SEQ ID NO: 4444

Sb04g006890

Sorghum

Polynucleotide
SEQ ID NO: 2081

bicolor

Polypeptide
SEQ ID NO: 2082

Genomic
SEQ ID NO: 4445

Sb04g006900

Sorghum

Polynucleotide
SEQ ID NO: 2083

bicolor

Polypeptide
SEQ ID NO: 2084

Genomic
SEQ ID NO: 4446

Sb04g006970

Sorghum

Polynucleotide
SEQ ID NO: 2085

bicolor

Polypeptide
SEQ ID NO: 2086

Genomic
SEQ ID NO: 4447

Sb04g007140

Sorghum

Polynucleotide
SEQ ID NO: 2087

bicolor

Polypeptide
SEQ ID NO: 2088

Genomic
SEQ ID NO: 4448

Sb04g007400

Sorghum

Polynucleotide
SEQ ID NO: 2089

bicolor

Polypeptide
SEQ ID NO: 2090

Genomic
SEQ ID NO: 4449

Sb04g007530

Sorghum

Polynucleotide
SEQ ID NO: 2091

bicolor

Polypeptide
SEQ ID NO: 2092

Genomic
SEQ ID NO: 4450

Sb04g008160

Sorghum

Polynucleotide
SEQ ID NO: 2093

bicolor

Polypeptide
SEQ ID NO: 2094

Genomic
SEQ ID NO: 4451

Sb04g008360

Sorghum

Polynucleotide
SEQ ID NO: 2095

bicolor

Polypeptide
SEQ ID NO: 2096

Genomic
SEQ ID NO: 4452

Sb04g008400

Sorghum

Polynucleotide
SEQ ID NO: 2097

bicolor

Polypeptide
SEQ ID NO: 2098

Genomic
SEQ ID NO: 4453

Sb04g008760

Sorghum

Polynucleotide
SEQ ID NO: 2099

bicolor

Polypeptide
SEQ ID NO: 2100

Genomic
SEQ ID NO: 4454

Sb04g008770

Sorghum

Polynucleotide
SEQ ID NO: 2101

bicolor

Polypeptide
SEQ ID NO: 2102

Genomic
SEQ ID NO: 4455

Sb04g009090

Sorghum

Polynucleotide
SEQ ID NO: 2103

bicolor

Polypeptide
SEQ ID NO: 2104

Genomic
SEQ ID NO: 4456

Sb04g009760

Sorghum

Polynucleotide
SEQ ID NO: 2105

bicolor

Polypeptide
SEQ ID NO: 2106

Genomic
SEQ ID NO: 4457

Sb04g010290

Sorghum

Polynucleotide
SEQ ID NO: 2107

bicolor

Polypeptide
SEQ ID NO: 2108

Genomic
SEQ ID NO: 4458

Sb04g010650

Sorghum

Polynucleotide
SEQ ID NO: 2109

bicolor

Polypeptide
SEQ ID NO: 2110

Genomic
SEQ ID NO: 4459

Sb04g010980

Sorghum

Polynucleotide
SEQ ID NO: 2111

bicolor

Polypeptide
SEQ ID NO: 2112

Genomic
SEQ ID NO: 4460

Sb04g011000

Sorghum

Polynucleotide
SEQ ID NO: 2113

bicolor

Polypeptide
SEQ ID NO: 2114

Genomic
SEQ ID NO: 4461

Sb04g011060

Sorghum

Polynucleotide
SEQ ID NO: 2115

bicolor

Polypeptide
SEQ ID NO: 2116

Genomic
SEQ ID NO: 4462

Sb04g011180

Sorghum

Polynucleotide
SEQ ID NO: 2117

bicolor

Polypeptide
SEQ ID NO: 2118

Genomic
SEQ ID NO: 4463

Sb04g012170

Sorghum

Polynucleotide
SEQ ID NO: 2119

bicolor

Polypeptide
SEQ ID NO: 2120

Genomic
SEQ ID NO: 4464

Sb04g012920

Sorghum

Polynucleotide
SEQ ID NO: 2121

bicolor

Polypeptide
SEQ ID NO: 2122

Genomic
SEQ ID NO: 4465

Sb04g013580

Sorghum

Polynucleotide
SEQ ID NO: 2123

bicolor

Polypeptide
SEQ ID NO: 2124

Genomic
SEQ ID NO: 4466

Sb04g014190

Sorghum

Polynucleotide
SEQ ID NO: 2125

bicolor

Polypeptide
SEQ ID NO: 2126

Genomic
SEQ ID NO: 4467

Sb04g017430

Sorghum

Polynucleotide
SEQ ID NO: 2127

bicolor

Polypeptide
SEQ ID NO: 2128

Genomic
SEQ ID NO: 4468

Sb04g020150

Sorghum

Polynucleotide
SEQ ID NO: 2129

bicolor

Polypeptide
SEQ ID NO: 2130

Genomic
SEQ ID NO: 4469

Sb04g020180

Sorghum

Polynucleotide
SEQ ID NO: 2131

bicolor

Polypeptide
SEQ ID NO: 2132

Genomic
SEQ ID NO: 4470

Sb04g020450

Sorghum

Polynucleotide
SEQ ID NO: 2133

bicolor

Polypeptide
SEQ ID NO: 2134

Genomic
SEQ ID NO: 4471

Sb04g020510

Sorghum

Polynucleotide
SEQ ID NO: 2135

bicolor

Polypeptide
SEQ ID NO: 2136

Genomic
SEQ ID NO: 4472

Sb04g021530

Sorghum

Polynucleotide
SEQ ID NO: 2137

bicolor

Polypeptide
SEQ ID NO: 2138

Genomic
SEQ ID NO: 4473

Sb04g021890

Sorghum

Polynucleotide
SEQ ID NO: 2139

bicolor

Polypeptide
SEQ ID NO: 2140

Genomic
SEQ ID NO: 4474

Sb04g122150

Sorghum

Polynucleotide
SEQ ID NO: 2141

bicolor

Polypeptide
SEQ ID NO: 2142

Genomic
SEQ ID NO: 4475

Sb04g022410

Sorghum

Polynucleotide
SEQ ID NO: 2143

bicolor

Polypeptide
SEQ ID NO: 2144

Genomic
SEQ ID NO: 4476

Sb04g022460

Sorghum

Polynucleotide
SEQ ID NO: 2145

bicolor

Polypeptide
SEQ ID NO: 2146

Genomic
SEQ ID NO: 4477

Sb04g022970

Sorghum

Polynucleotide
SEQ ID NO: 2147

bicolor

Polypeptide
SEQ ID NO: 2148

Genomic
SEQ ID NO: 4478

Sb04g023000

Sorghum

Polynucleotide
SEQ ID NO: 2149

bicolor

Polypeptide
SEQ ID NO: 2150

Genomic
SEQ ID NO: 4479

Sb04g023020

Sorghum

Polynucleotide
SEQ ID NO: 2151

bicolor

Polypeptide
SEQ ID NO: 2152

Genomic
SEQ ID NO: 4480

Sb04g023130

Sorghum

Polynucleotide
SEQ ID NO: 2153

bicolor

Polypeptide
SEQ ID NO: 2154

Genomic
SEQ ID NO: 4481

Sb04g023390

Sorghum

Polynucleotide
SEQ ID NO: 2155

bicolor

Polypeptide
SEQ ID NO: 2156

Genomic
SEQ ID NO: 4482

Sb04g023750

Sorghum

Polynucleotide
SEQ ID NO: 2157

bicolor

Polypeptide
SEQ ID NO: 2158

Genomic
SEQ ID NO: 4483

Sb04g023870

Sorghum

Polynucleotide
SEQ ID NO: 2159

bicolor

Polypeptide
SEQ ID NO: 2160

Genomic
SEQ ID NO: 4484

Sb04g024270

Sorghum

Polynucleotide
SEQ ID NO: 2161

bicolor

Polypeptide
SEQ ID NO: 2162

Genomic
SEQ ID NO: 4485

Sb04g024390

Sorghum

Polynucleotide
SEQ ID NO: 2163

bicolor

Polypeptide
SEQ ID NO: 2164

Genomic
SEQ ID NO: 4486

Sb04g024490

Sorghum

Polynucleotide
SEQ ID NO: 2165

bicolor

Polypeptide
SEQ ID NO: 2166

Genomic
SEQ ID NO: 4487

Sb04g024500

Sorghum

Polynucleotide
SEQ ID NO: 2167

bicolor

Polypeptide
SEQ ID NO: 2168

Genomic
SEQ ID NO: 4488

Sb04g024570

Sorghum

Polynucleotide
SEQ ID NO: 2169

bicolor

Polypeptide
SEQ ID NO: 2170

Genomic
SEQ ID NO: 4489

Sb04g024880

Sorghum

Polynucleotide
SEQ ID NO: 2171

bicolor

Polypeptide
SEQ ID NO: 2172

Genomic
SEQ ID NO: 4490

Sb04g025260

Sorghum

Polynucleotide
SEQ ID NO: 2173

bicolor

Polypeptide
SEQ ID NO: 2174

Genomic
SEQ ID NO: 4491

Sb04g025500

Sorghum

Polynucleotide
SEQ ID NO: 2175

bicolor

Polypeptide
SEQ ID NO: 2176

Genomic
SEQ ID NO: 4492

Sb04g025870

Sorghum

Polynucleotide
SEQ ID NO: 2177

bicolor

Polypeptide
SEQ ID NO: 2178

Genomic
SEQ ID NO: 4493

Sb04g025910

Sorghum

Polynucleotide
SEQ ID NO: 2179

bicolor

Polypeptide
SEQ ID NO: 2180

Genomic
SEQ ID NO: 4494

Sb04g025960

Sorghum

Polynucleotide
SEQ ID NO: 2181

bicolor

Polypeptide
SEQ ID NO: 2182

Genomic
SEQ ID NO: 4495

Sb04g026020

Sorghum

Polynucleotide
SEQ ID NO: 2183

bicolor

Polypeptide
SEQ ID NO: 2184

Genomic
SEQ ID NO: 4496

Sb04g026030

Sorghum

Polynucleotide
SEQ ID NO: 2185

bicolor

Polypeptide
SEQ ID NO: 2186

Genomic
SEQ ID NO: 4497

Sb04g026320

Sorghum

Polynucleotide
SEQ ID NO: 2187

bicolor

Polypeptide
SEQ ID NO: 2188

Genomic
SEQ ID NO: 4498

Sb04g129820

Sorghum

Polynucleotide
SEQ ID NO: 2189

bicolor

Polypeptide
SEQ ID NO: 2190

Genomic
SEQ ID NO: 4499

Sb04g026440

Sorghum

Polynucleotide
SEQ ID NO: 2191

bicolor

Polypeptide
SEQ ID NO: 2192

Genomic
SEQ ID NO: 4500

Sb04g026750

Sorghum

Polynucleotide
SEQ ID NO: 2193

bicolor

Polypeptide
SEQ ID NO: 2194

Genomic
SEQ ID NO: 4501

Sb04g026950

Sorghum

Polynucleotide
SEQ ID NO: 2195

bicolor

Polypeptide
SEQ ID NO: 2196

Genomic
SEQ ID NO: 4502

Sb04g027620

Sorghum

Polynucleotide
SEQ ID NO: 2197

bicolor

Polypeptide
SEQ ID NO: 2198

Genomic
SEQ ID NO: 4503

Sb04g027800

Sorghum

Polynucleotide
SEQ ID NO: 2199

bicolor

Polypeptide
SEQ ID NO: 2200

Genomic
SEQ ID NO: 4504

Sb04g027880

Sorghum

Polynucleotide
SEQ ID NO: 2201

bicolor

Polypeptide
SEQ ID NO: 2202

Genomic
SEQ ID NO: 4505

Sb04g027980

Sorghum

Polynucleotide
SEQ ID NO: 2203

bicolor

Polypeptide
SEQ ID NO: 2204

Genomic
SEQ ID NO: 4506

Sb04g028070

Sorghum

Polynucleotide
SEQ ID NO: 2205

bicolor

Polypeptide
SEQ ID NO: 2206

Genomic
SEQ ID NO: 4507

Sb04g028210

Sorghum

Polynucleotide
SEQ ID NO: 2207

bicolor

Polypeptide
SEQ ID NO: 2208

Genomic
SEQ ID NO: 4508

Sb04g028440

Sorghum

Polynucleotide
SEQ ID NO: 2209

bicolor

Polypeptide
SEQ ID NO: 2210

Genomic
SEQ ID NO: 4509

Sb04g028450

Sorghum

Polynucleotide
SEQ ID NO: 2211

bicolor

Polypeptide
SEQ ID NO: 2212

Genomic
SEQ ID NO: 4510

Sb04g028690

Sorghum

Polynucleotide
SEQ ID NO: 2213

bicolor

Polypeptide
SEQ ID NO: 2214

Genomic
SEQ ID NO: 4511

Sb04g028740

Sorghum

Polynucleotide
SEQ ID NO: 2215

bicolor

Polypeptide
SEQ ID NO: 2216

Genomic
SEQ ID NO: 4512

Sb04g028760

Sorghum

Polynucleotide
SEQ ID NO: 2217

bicolor

Polypeptide
SEQ ID NO: 2218

Genomic
SEQ ID NO: 4513

Sb04g028810

Sorghum

Polynucleotide
SEQ ID NO: 2219

bicolor

Polypeptide
SEQ ID NO: 2220

Genomic
SEQ ID NO: 4514

Sb04g028980

Sorghum

Polynucleotide
SEQ ID NO: 2221

bicolor

Polypeptide
SEQ ID NO: 2222

Genomic
SEQ ID NO: 4515

Sb04g029000

Sorghum

Polynucleotide
SEQ ID NO: 2223

bicolor

Polypeptide
SEQ ID NO: 2224

Genomic
SEQ ID NO: 4516

Sb04g029020

Sorghum

Polynucleotide
SEQ ID NO: 2225

bicolor

Polypeptide
SEQ ID NO: 2226

Genomic
SEQ ID NO: 4517

Sb04g029030

Sorghum

Polynucleotide
SEQ ID NO: 2227

bicolor

Polypeptide
SEQ ID NO: 2228

Genomic
SEQ ID NO: 4518

Sb04g029410

Sorghum

Polynucleotide
SEQ ID NO: 2229

bicolor

Polypeptide
SEQ ID NO: 2230

Genomic
SEQ ID NO: 4519

Sb04g029660

Sorghum

Polynucleotide
SEQ ID NO: 2231

bicolor

Polypeptide
SEQ ID NO: 2232

Genomic
SEQ ID NO: 4520

Sb04g029810

Sorghum

Polynucleotide
SEQ ID NO: 2233

bicolor

Polypeptide
SEQ ID NO: 2234

Genomic
SEQ ID NO: 4521

Sb04g029850

Sorghum

Polynucleotide
SEQ ID NO: 2235

bicolor

Polypeptide
SEQ ID NO: 2236

Genomic
SEQ ID NO: 4522

Sb04g029920

Sorghum

Polynucleotide
SEQ ID NO: 2237

bicolor

Polypeptide
SEQ ID NO: 2238

Genomic
SEQ ID NO: 4523

Sb04g029940

Sorghum

Polynucleotide
SEQ ID NO: 2239

bicolor

Polypeptide
SEQ ID NO: 2240

Genomic
SEQ ID NO: 4524

Sb04g030530

Sorghum

Polynucleotide
SEQ ID NO: 2241

bicolor

Polypeptide
SEQ ID NO: 2242

Genomic
SEQ ID NO: 4525

Sb04g030560

Sorghum

Polynucleotide
SEQ ID NO: 2243

bicolor

Polypeptide
SEQ ID NO: 2244

Genomic
SEQ ID NO: 4526

Sb04g030700

Sorghum

Polynucleotide
SEQ ID NO: 2245

bicolor

Polypeptide
SEQ ID NO: 2246

Genomic
SEQ ID NO: 4527

Sb04g030830

Sorghum

Polynucleotide
SEQ ID NO: 2247

bicolor

Polypeptide
SEQ ID NO: 2248

Genomic
SEQ ID NO: 4528

Sb04g030840

Sorghum

Polynucleotide
SEQ ID NO: 2249

bicolor

Polypeptide
SEQ ID NO: 2250

Genomic
SEQ ID NO: 4529

Sb04g030895

Sorghum

Polynucleotide
SEQ ID NO: 2251

bicolor

Polypeptide
SEQ ID NO: 2252

Genomic
SEQ ID NO: 4530

Sb04g031600

Sorghum

Polynucleotide
SEQ ID NO: 2253

bicolor

Polypeptide
SEQ ID NO: 2254

Genomic
SEQ ID NO: 4531

Sb04g031750

Sorghum

Polynucleotide
SEQ ID NO: 2255

bicolor

Polypeptide
SEQ ID NO: 2256

Genomic
SEQ ID NO: 4532

Sb01g049305

Sorghum

Polynucleotide
SEQ ID NO: 2257

bicolor

Polypeptide
SEQ ID NO: 2258

Genomic
SEQ ID NO: 4533

Sb04g032840

Sorghum

Polynucleotide
SEQ ID NO: 2259

bicolor

Polypeptide
SEQ ID NO: 2260

Genomic
SEQ ID NO: 4534

Sb04g032880

Sorghum

Polynucleotide
SEQ ID NO: 2261

bicolor

Polypeptide
SEQ ID NO: 2262

Genomic
SEQ ID NO: 4535

Sb04g033000

Sorghum

Polynucleotide
SEQ ID NO: 2263

bicolor

Polypeptide
SEQ ID NO: 2264

Genomic
SEQ ID NO: 4536

Sb04g140630

Sorghum

Polynucleotide
SEQ ID NO: 2265

bicolor

Polypeptide
SEQ ID NO: 2266

Genomic
SEQ ID NO: 4537

Sb04g140640

Sorghum

Polynucleotide
SEQ ID NO: 2267

bicolor

Polypeptide
SEQ ID NO: 2268

Genomic
SEQ ID NO: 4538

Sb04g140670

Sorghum

Polynucleotide
SEQ ID NO: 2269

bicolor

Polypeptide
SEQ ID NO: 2270

Genomic
SEQ ID NO: 4539

Sb04g033340

Sorghum

Polynucleotide
SEQ ID NO: 2271

bicolor

Polypeptide
SEQ ID NO: 2272

Genomic
SEQ ID NO: 4540

Sb04g033370

Sorghum

Polynucleotide
SEQ ID NO: 2273

bicolor

Polypeptide
SEQ ID NO: 2274

Genomic
SEQ ID NO: 4541

Sb04g033570

Sorghum

Polynucleotide
SEQ ID NO: 2275

bicolor

Polypeptide
SEQ ID NO: 2276

Genomic
SEQ ID NO: 4542

Sb04g033700

Sorghum

Polynucleotide
SEQ ID NO: 2277

bicolor

Polypeptide
SEQ ID NO: 2278

Genomic
SEQ ID NO: 4543

Sb04g033710

Sorghum

Polynucleotide
SEQ ID NO: 2279

bicolor

Polypeptide
SEQ ID NO: 2280

Genomic
SEQ ID NO: 4544

Sb04g033895

Sorghum

Polynucleotide
SEQ ID NO: 2281

bicolor

Polypeptide
SEQ ID NO: 2282

Genomic
SEQ ID NO: 4545

Sb04g141710

Sorghum

Polynucleotide
SEQ ID NO: 2283

bicolor

Polypeptide
SEQ ID NO: 2284

Genomic
SEQ ID NO: 4546

Sb04g034056

Sorghum

Polynucleotide
SEQ ID NO: 2285

bicolor

Polypeptide
SEQ ID NO: 2286

Genomic
SEQ ID NO: 4547

Sb04g034130

Sorghum

Polynucleotide
SEQ ID NO: 2287

bicolor

Polypeptide
SEQ ID NO: 2288

Genomic
SEQ ID NO: 4548

Sb04g034136

Sorghum

Polynucleotide
SEQ ID NO: 2289

bicolor

Polypeptide
SEQ ID NO: 2290

Genomic
SEQ ID NO: 4549

Sb04g141930

Sorghum

Polynucleotide
SEQ ID NO: 2291

bicolor

Polypeptide
SEQ ID NO: 2292

Genomic
SEQ ID NO: 4550

Sb04g034440

Sorghum

Polynucleotide
SEQ ID NO: 2293

bicolor

Polypeptide
SEQ ID NO: 2294

Genomic
SEQ ID NO: 4551

Sb04g034540

Sorghum

Polynucleotide
SEQ ID NO: 2295

bicolor

Polypeptide
SEQ ID NO: 2296

Genomic
SEQ ID NO: 4552

Sb04g034590

Sorghum

Polynucleotide
SEQ ID NO: 2297

bicolor

Polypeptide
SEQ ID NO: 2298

Genomic
SEQ ID NO: 4553

Sb04g034650

Sorghum

Polynucleotide
SEQ ID NO: 2299

bicolor

Polypeptide
SEQ ID NO: 2300

Genomic
SEQ ID NO: 4554

Sb04g034700

Sorghum

Polynucleotide
SEQ ID NO: 2301

bicolor

Polypeptide
SEQ ID NO: 2302

Genomic
SEQ ID NO: 4555

Sb04g142920

Sorghum

Polynucleotide
SEQ ID NO: 2303

bicolor

Polypeptide
SEQ ID NO: 2304

Genomic
SEQ ID NO: 4556

Sb04g142930

Sorghum

Polynucleotide
SEQ ID NO: 2305

bicolor

Polypeptide
SEQ ID NO: 2306

Genomic
SEQ ID NO: 4557

Sb04g035180

Sorghum

Polynucleotide
SEQ ID NO: 2307

bicolor

Polypeptide
SEQ ID NO: 2308

Genomic
SEQ ID NO: 4558

Sb04g035290

Sorghum

Polynucleotide
SEQ ID NO: 2309

bicolor

Polypeptide
SEQ ID NO: 2310

Genomic
SEQ ID NO: 4559

Sb04g035420

Sorghum

Polynucleotide
SEQ ID NO: 2311

bicolor

Polypeptide
SEQ ID NO: 2312

Genomic
SEQ ID NO: 4560

Sb04g035610

Sorghum

Polynucleotide
SEQ ID NO: 2313

bicolor

Polypeptide
SEQ ID NO: 2314

Genomic
SEQ ID NO: 4561

Sb04g035690

Sorghum

Polynucleotide
SEQ ID NO: 2315

bicolor

Polypeptide
SEQ ID NO: 2316

Genomic
SEQ ID NO: 4562

Sb04g035980

Sorghum

Polynucleotide
SEQ ID NO: 2317

bicolor

Polypeptide
SEQ ID NO: 2318

Genomic
SEQ ID NO: 4563

Sb04g035990

Sorghum

Polynucleotide
SEQ ID NO: 2319

bicolor

Polypeptide
SEQ ID NO: 2320

Genomic
SEQ ID NO: 4564

Sb04g036050

Sorghum

Polynucleotide
SEQ ID NO: 2321

bicolor

Polypeptide
SEQ ID NO: 2322

Genomic
SEQ ID NO: 4565

Sb04g036120

Sorghum

Polynucleotide
SEQ ID NO: 2323

bicolor

Polypeptide
SEQ ID NO: 2324

Genomic
SEQ ID NO: 4566

Sb04g036640

Sorghum

Polynucleotide
SEQ ID NO: 2325

bicolor

Polypeptide
SEQ ID NO: 2326

Genomic
SEQ ID NO: 4567

Sb04g146060

Sorghum

Polynucleotide
SEQ ID NO: 2327

bicolor

Polypeptide
SEQ ID NO: 2328

Genomic
SEQ ID NO: 4568

Sb04g036700

Sorghum

Polynucleotide
SEQ ID NO: 2329

bicolor

Polypeptide
SEQ ID NO: 2330

Genomic
SEQ ID NO: 4569

Sb04g036850

Sorghum

Polynucleotide
SEQ ID NO: 2331

bicolor

Polypeptide
SEQ ID NO: 2332

Genomic
SEQ ID NO: 4570

Sb04g036930

Sorghum

Polynucleotide
SEQ ID NO: 2333

bicolor

Polypeptide
SEQ ID NO: 2334

Genomic
SEQ ID NO: 4571

Sb04g146540

Sorghum

Polynucleotide
SEQ ID NO: 2335

bicolor

Polypeptide
SEQ ID NO: 2336

Genomic
SEQ ID NO: 4572

Sb04g037140

Sorghum

Polynucleotide
SEQ ID NO: 2337

bicolor

Polypeptide
SEQ ID NO: 2338

Genomic
SEQ ID NO: 4573

Sb04g037160

Sorghum

Polynucleotide
SEQ ID NO: 2339

bicolor

Polypeptide
SEQ ID NO: 2340

Genomic
SEQ ID NO: 4574

Sb04g037280

Sorghum

Polynucleotide
SEQ ID NO: 2341

bicolor

Polypeptide
SEQ ID NO: 2342

Genomic
SEQ ID NO: 4575

Sb04g037730

Sorghum

Polynucleotide
SEQ ID NO: 2343

bicolor

Polypeptide
SEQ ID NO: 2344

Genomic
SEQ ID NO: 4576

Sb04g037740

Sorghum

Polynucleotide
SEQ ID NO: 2345

bicolor

Polypeptide
SEQ ID NO: 2346

Genomic
SEQ ID NO: 4577

Sb04g038060

Sorghum

Polynucleotide
SEQ ID NO: 2347

bicolor

Polypeptide
SEQ ID NO: 2348

Genomic
SEQ ID NO: 4578

Sb04g038190

Sorghum

Polynucleotide
SEQ ID NO: 2349

bicolor

Polypeptide
SEQ ID NO: 2350

Genomic
SEQ ID NO: 4579

Sb04g038290

Sorghum

Polynucleotide
SEQ ID NO: 2351

bicolor

Polypeptide
SEQ ID NO: 2352

Genomic
SEQ ID NO: 4580

Sb04g038630

Sorghum

Polynucleotide
SEQ ID NO: 2353

bicolor

Polypeptide
SEQ ID NO: 2354

Genomic
SEQ ID NO: 4581

Sb04g038640

Sorghum

Polynucleotide
SEQ ID NO: 2355

bicolor

Polypeptide
SEQ ID NO: 2356

Genomic
SEQ ID NO: 4582

Sb0506s002020

Sorghum

Polynucleotide
SEQ ID NO: 2357

bicolor

Polypeptide
SEQ ID NO: 2358

Genomic
SEQ ID NO: 4583

Sb05g000210

Sorghum

Polynucleotide
SEQ ID NO: 2359

bicolor

Polypeptide
SEQ ID NO: 2360

Genomic
SEQ ID NO: 4584

Sb05g000390

Sorghum

Polynucleotide
SEQ ID NO: 2361

bicolor

Polypeptide
SEQ ID NO: 2362

Genomic
SEQ ID NO: 4585

Sb05g000480

Sorghum

Polynucleotide
SEQ ID NO: 2363

bicolor

Polypeptide
SEQ ID NO: 2364

Genomic
SEQ ID NO: 4586

Sb05g000630

Sorghum

Polynucleotide
SEQ ID NO: 2365

bicolor

Polypeptide
SEQ ID NO: 2366

Genomic
SEQ ID NO: 4587

Sb05g001080

Sorghum

Polynucleotide
SEQ ID NO: 2367

bicolor

Polypeptide
SEQ ID NO: 2368

Genomic
SEQ ID NO: 4588

Sb05g001170

Sorghum

Polynucleotide
SEQ ID NO: 2369

bicolor

Polypeptide
SEQ ID NO: 2370

Genomic
SEQ ID NO: 4589

Sb05g001400

Sorghum

Polynucleotide
SEQ ID NO: 2371

bicolor

Polypeptide
SEQ ID NO: 2372

Genomic
SEQ ID NO: 4590

Sb05g002420

Sorghum

Polynucleotide
SEQ ID NO: 2373

bicolor

Polypeptide
SEQ ID NO: 2374

Genomic
SEQ ID NO: 4591

Sb05g003480

Sorghum

Polynucleotide
SEQ ID NO: 2375

bicolor

Polypeptide
SEQ ID NO: 2376

Genomic
SEQ ID NO: 4592

Sb05g003740

Sorghum

Polynucleotide
SEQ ID NO: 2377

bicolor

Polypeptide
SEQ ID NO: 2378

Genomic
SEQ ID NO: 4593

Sb05g003870

Sorghum

Polynucleotide
SEQ ID NO: 2379

bicolor

Polypeptide
SEQ ID NO: 2380

Genomic
SEQ ID NO: 4594

Sb05g004860

Sorghum

Polynucleotide
SEQ ID NO: 2381

bicolor

Polypeptide
SEQ ID NO: 2382

Genomic
SEQ ID NO: 4595

Sb05g005021

Sorghum

Polynucleotide
SEQ ID NO: 2383

bicolor

Polypeptide
SEQ ID NO: 2384

Genomic
SEQ ID NO: 4596

Sb05g005390

Sorghum

Polynucleotide
SEQ ID NO: 2385

bicolor

Polypeptide
SEQ ID NO: 2386

Genomic
SEQ ID NO: 4597

Sb05g005470

Sorghum

Polynucleotide
SEQ ID NO: 2387

bicolor

Polypeptide
SEQ ID NO: 2388

Genomic
SEQ ID NO: 4598

Sb05g006150

Sorghum

Polynucleotide
SEQ ID NO: 2389

bicolor

Polypeptide
SEQ ID NO: 2390

Genomic
SEQ ID NO: 4599

Sb05g006580

Sorghum

Polynucleotide
SEQ ID NO: 2391

bicolor

Polypeptide
SEQ ID NO: 2392

Genomic
SEQ ID NO: 4600

Sb05g006690

Sorghum

Polynucleotide
SEQ ID NO: 2393

bicolor

Polypeptide
SEQ ID NO: 2394

Genomic
SEQ ID NO: 4601

Sb05g006840

Sorghum

Polynucleotide
SEQ ID NO: 2395

bicolor

Polypeptide
SEQ ID NO: 2396

Genomic
SEQ ID NO: 4602

Sb05g007000

Sorghum

Polynucleotide
SEQ ID NO: 2397

bicolor

Polypeptide
SEQ ID NO: 2398

Genomic
SEQ ID NO: 4603

Sb08g021890

Sorghum

Polynucleotide
SEQ ID NO: 2399

bicolor

Polypeptide
SEQ ID NO: 2400

Genomic
SEQ ID NO: 4604

Sb05g008512

Sorghum

Polynucleotide
SEQ ID NO: 2401

bicolor

Polypeptide
SEQ ID NO: 2402

Genomic
SEQ ID NO: 4605

Sb05g008670

Sorghum

Polynucleotide
SEQ ID NO: 2403

bicolor

Polypeptide
SEQ ID NO: 2404

Genomic
SEQ ID NO: 4606

Sb05g008690

Sorghum

Polynucleotide
SEQ ID NO: 2405

bicolor

Polypeptide
SEQ ID NO: 2406

Genomic
SEQ ID NO: 4607

Sb05g008830

Sorghum

Polynucleotide
SEQ ID NO: 2407

bicolor

Polypeptide
SEQ ID NO: 2408

Genomic
SEQ ID NO: 4608

Sb05g009120

Sorghum

Polynucleotide
SEQ ID NO: 2409

bicolor

Polypeptide
SEQ ID NO: 2410

Genomic
SEQ ID NO: 4609

Sb05g009430

Sorghum

Polynucleotide
SEQ ID NO: 2411

bicolor

Polypeptide
SEQ ID NO: 2412

Genomic
SEQ ID NO: 4610

Sb05g016800

Sorghum

Polynucleotide
SEQ ID NO: 2413

bicolor

Polypeptide
SEQ ID NO: 2414

Genomic
SEQ ID NO: 4611

Sb05g017940

Sorghum

Polynucleotide
SEQ ID NO: 2415

bicolor

Polypeptide
SEQ ID NO: 2416

Genomic
SEQ ID NO: 4612

Sb05g017970

Sorghum

Polynucleotide
SEQ ID NO: 2417

bicolor

Polypeptide
SEQ ID NO: 2418

Genomic
SEQ ID NO: 4613

Sb05g018080

Sorghum

Polynucleotide
SEQ ID NO: 2419

bicolor

Polypeptide
SEQ ID NO: 2420

Genomic
SEQ ID NO: 4614

Sb05g001130

Sorghum

Polynucleotide
SEQ ID NO: 2421

bicolor

Polypeptide
SEQ ID NO: 2422

Genomic
SEQ ID NO: 4615

Sb05g018890

Sorghum

Polynucleotide
SEQ ID NO: 2423

bicolor

Polypeptide
SEQ ID NO: 2424

Genomic
SEQ ID NO: 4616

Sb05g020370

Sorghum

Polynucleotide
SEQ ID NO: 2425

bicolor

Polypeptide
SEQ ID NO: 2426

Genomic
SEQ ID NO: 4617

Sb05g020780

Sorghum

Polynucleotide
SEQ ID NO: 2427

bicolor

Polypeptide
SEQ ID NO: 2428

Genomic
SEQ ID NO: 4618

Sb05g021000

Sorghum

Polynucleotide
SEQ ID NO: 2429

bicolor

Polypeptide
SEQ ID NO: 2430

Genomic
SEQ ID NO: 4619

Sb05g021240

Sorghum

Polynucleotide
SEQ ID NO: 2431

bicolor

Polypeptide
SEQ ID NO: 2432

Genomic
SEQ ID NO: 4620

Sb05g021740

Sorghum

Polynucleotide
SEQ ID NO: 2433

bicolor

Polypeptide
SEQ ID NO: 2434

Genomic
SEQ ID NO: 4621

Sb05g023600

Sorghum

Polynucleotide
SEQ ID NO: 2435

bicolor

Polypeptide
SEQ ID NO: 2436

Genomic
SEQ ID NO: 4622

Sb05g024020

Sorghum

Polynucleotide
SEQ ID NO: 2437

bicolor

Polypeptide
SEQ ID NO: 2438

Genomic
SEQ ID NO: 4623

Sb05g024160

Sorghum

Polynucleotide
SEQ ID NO: 2439

bicolor

Polypeptide
SEQ ID NO: 2440

Genomic
SEQ ID NO: 4624

Sb05g151440

Sorghum

Polynucleotide
SEQ ID NO: 2441

bicolor

Polypeptide
SEQ ID NO: 2442

Genomic
SEQ ID NO: 4625

Sb05g024490

Sorghum

Polynucleotide
SEQ ID NO: 2443

bicolor

Polypeptide
SEQ ID NO: 2444

Genomic
SEQ ID NO: 4626

Sb05g024850

Sorghum

Polynucleotide
SEQ ID NO: 2445

bicolor

Polypeptide
SEQ ID NO: 2446

Genomic
SEQ ID NO: 4627

Sb05g025170

Sorghum

Polynucleotide
SEQ ID NO: 2447

bicolor

Polypeptide
SEQ ID NO: 2448

Genomic
SEQ ID NO: 4628

Sb05g025210

Sorghum

Polynucleotide
SEQ ID NO: 2449

bicolor

Polypeptide
SEQ ID NO: 2450

Genomic
SEQ ID NO: 4629

Sb05g025710

Sorghum

Polynucleotide
SEQ ID NO: 2451

bicolor

Polypeptide
SEQ ID NO: 2452

Genomic
SEQ ID NO: 4630

Sb05g025860

Sorghum

Polynucleotide
SEQ ID NO: 2453

bicolor

Polypeptide
SEQ ID NO: 2454

Genomic
SEQ ID NO: 4631

Sb05g025970

Sorghum

Polynucleotide
SEQ ID NO: 2455

bicolor

Polypeptide
SEQ ID NO: 2456

Genomic
SEQ ID NO: 4632

Sb05g026750

Sorghum

Polynucleotide
SEQ ID NO: 2457

bicolor

Polypeptide
SEQ ID NO: 2458

Genomic
SEQ ID NO: 4633

Sb05g022300

Sorghum

Polynucleotide
SEQ ID NO: 2459

bicolor

Polypeptide
SEQ ID NO: 2460

Genomic
SEQ ID NO: 4634

Sb05g026950

Sorghum

Polynucleotide
SEQ ID NO: 2461

bicolor

Polypeptide
SEQ ID NO: 2462

Genomic
SEQ ID NO: 4635

Sb05g158230

Sorghum

Polynucleotide
SEQ ID NO: 2463

bicolor

Polypeptide
SEQ ID NO: 2464

Genomic
SEQ ID NO: 4636

Sb05g027340

Sorghum

Polynucleotide
SEQ ID NO: 2465

bicolor

Polypeptide
SEQ ID NO: 2466

Genomic
SEQ ID NO: 4637

Sb05g027480

Sorghum

Polynucleotide
SEQ ID NO: 2467

bicolor

Polypeptide
SEQ ID NO: 2468

Genomic
SEQ ID NO: 4638

Sb05g027820

Sorghum

Polynucleotide
SEQ ID NO: 2469

bicolor

Polypeptide
SEQ ID NO: 2470

Genomic
SEQ ID NO: 4639

Sb05g027890

Sorghum

Polynucleotide
SEQ ID NO: 2471

bicolor

Polypeptide
SEQ ID NO: 2472

Genomic
SEQ ID NO: 4640

Sb0612s002010

Sorghum

Polynucleotide
SEQ ID NO: 2473

bicolor

Polypeptide
SEQ ID NO: 2474

Genomic
SEQ ID NO: 4641

Sb06g000310

Sorghum

Polynucleotide
SEQ ID NO: 2475

bicolor

Polypeptide
SEQ ID NO: 2476

Genomic
SEQ ID NO: 4642

Sb06g001140

Sorghum

Polynucleotide
SEQ ID NO: 2477

bicolor

Polypeptide
SEQ ID NO: 2478

Genomic
SEQ ID NO: 4643

Sb06g001740

Sorghum

Polynucleotide
SEQ ID NO: 2479

bicolor

Polypeptide
SEQ ID NO: 2480

Genomic
SEQ ID NO: 4644

Sb06g001890

Sorghum

Polynucleotide
SEQ ID NO: 2481

bicolor

Polypeptide
SEQ ID NO: 2482

Genomic
SEQ ID NO: 4645

Sb06g002500

Sorghum

Polynucleotide
SEQ ID NO: 2483

bicolor

Polypeptide
SEQ ID NO: 2484

Genomic
SEQ ID NO: 4646

Sb06g003280

Sorghum

Polynucleotide
SEQ ID NO: 2485

bicolor

Polypeptide
SEQ ID NO: 2486

Genomic
SEQ ID NO: 4647

Sb06g004750

Sorghum

Polynucleotide
SEQ ID NO: 2487

bicolor

Polypeptide
SEQ ID NO: 2488

Genomic
SEQ ID NO: 4648

Sb06g011765

Sorghum

Polynucleotide
SEQ ID NO: 2489

bicolor

Polypeptide
SEQ ID NO: 2490

Genomic
SEQ ID NO: 4649

Sb06g013750

Sorghum

Polynucleotide
SEQ ID NO: 2491

bicolor

Polypeptide
SEQ ID NO: 2492

Genomic
SEQ ID NO: 4650

Sb06g013790

Sorghum

Polynucleotide
SEQ ID NO: 2493

bicolor

Polypeptide
SEQ ID NO: 2494

Genomic
SEQ ID NO: 4651

Sb06g013860

Sorghum

Polynucleotide
SEQ ID NO: 2495

bicolor

Polypeptide
SEQ ID NO: 2496

Genomic
SEQ ID NO: 4652

Sb06g014220

Sorghum

Polynucleotide
SEQ ID NO: 2497

bicolor

Polypeptide
SEQ ID NO: 2498

Genomic
SEQ ID NO: 4653

Sb06g014330

Sorghum

Polynucleotide
SEQ ID NO: 2499

bicolor

Polypeptide
SEQ ID NO: 2500

Genomic
SEQ ID NO: 4654

Sb06g014710

Sorghum

Polynucleotide
SEQ ID NO: 2501

bicolor

Polypeptide
SEQ ID NO: 2502

Genomic
SEQ ID NO: 4655

Sb06g014740

Sorghum

Polynucleotide
SEQ ID NO: 2503

bicolor

Polypeptide
SEQ ID NO: 2504

Genomic
SEQ ID NO: 4656

Sb06g014890

Sorghum

Polynucleotide
SEQ ID NO: 2505

bicolor

Polypeptide
SEQ ID NO: 2506

Genomic
SEQ ID NO: 4657

Sb06g015080

Sorghum

Polynucleotide
SEQ ID NO: 2507

bicolor

Polypeptide
SEQ ID NO: 2508

Genomic
SEQ ID NO: 4658

Sb06g015150

Sorghum

Polynucleotide
SEQ ID NO: 2509

bicolor

Polypeptide
SEQ ID NO: 2510

Genomic
SEQ ID NO: 4659

Sb06g015230

Sorghum

Polynucleotide
SEQ ID NO: 2511

bicolor

Polypeptide
SEQ ID NO: 2512

Genomic
SEQ ID NO: 4660

Sb06g015260

Sorghum

Polynucleotide
SEQ ID NO: 2513

bicolor

Polypeptide
SEQ ID NO: 2514

Genomic
SEQ ID NO: 4661

Sb06g015360

Sorghum

Polynucleotide
SEQ ID NO: 2515

bicolor

Polypeptide
SEQ ID NO: 2516

Genomic
SEQ ID NO: 4662

Sb06g114730

Sorghum

Polynucleotide
SEQ ID NO: 2517

bicolor

Polypeptide
SEQ ID NO: 2518

Genomic
SEQ ID NO: 4663

Sb06g015490

Sorghum

Polynucleotide
SEQ ID NO: 2519

bicolor

Polypeptide
SEQ ID NO: 2520

Genomic
SEQ ID NO: 4664

Sb06g015550

Sorghum

Polynucleotide
SEQ ID NO: 2521

bicolor

Polypeptide
SEQ ID NO: 2522

Genomic
SEQ ID NO: 4665

Sb06g016070

Sorghum

Polynucleotide
SEQ ID NO: 2523

bicolor

Polypeptide
SEQ ID NO: 2524

Genomic
SEQ ID NO: 4666

Sb06g016110

Sorghum

Polynucleotide
SEQ ID NO: 2525

bicolor

Polypeptide
SEQ ID NO: 2526

Genomic
SEQ ID NO: 4667

Sb06g016230

Sorghum

Polynucleotide
SEQ ID NO: 2527

bicolor

Polypeptide
SEQ ID NO: 2528

Genomic
SEQ ID NO: 4668

Sb06g016420

Sorghum

Polynucleotide
SEQ ID NO: 2529

bicolor

Polypeptide
SEQ ID NO: 2530

Genomic
SEQ ID NO: 4669

Sb06g016920

Sorghum

Polynucleotide
SEQ ID NO: 2531

bicolor

Polypeptide
SEQ ID NO: 2532

Genomic
SEQ ID NO: 4670

Sb06g017090

Sorghum

Polynucleotide
SEQ ID NO: 2533

bicolor

Polypeptide
SEQ ID NO: 2534

Genomic
SEQ ID NO: 4671

Sb06g017380

Sorghum

Polynucleotide
SEQ ID NO: 2535

bicolor

Polypeptide
SEQ ID NO: 2536

Genomic
SEQ ID NO: 4672

Sb06g017540

Sorghum

Polynucleotide
SEQ ID NO: 2537

bicolor

Polypeptide
SEQ ID NO: 2538

Genomic
SEQ ID NO: 4673

Sb06g017620

Sorghum

Polynucleotide
SEQ ID NO: 2539

bicolor

Polypeptide
SEQ ID NO: 2540

Genomic
SEQ ID NO: 4674

Sb06g017640

Sorghum

Polynucleotide
SEQ ID NO: 2541

bicolor

Polypeptide
SEQ ID NO: 2542

Genomic
SEQ ID NO: 4675

Sb06g018070

Sorghum

Polynucleotide
SEQ ID NO: 2543

bicolor

Polypeptide
SEQ ID NO: 2544

Genomic
SEQ ID NO: 4676

Sb06g018220

Sorghum

Polynucleotide
SEQ ID NO: 2545

bicolor

Polypeptide
SEQ ID NO: 2546

Genomic
SEQ ID NO: 4677

Sb06g018590

Sorghum

Polynucleotide
SEQ ID NO: 2547

bicolor

Polypeptide
SEQ ID NO: 2548

Genomic
SEQ ID NO: 4678

Sb06g018640

Sorghum

Polynucleotide
SEQ ID NO: 2549

bicolor

Polypeptide
SEQ ID NO: 2550

Genomic
SEQ ID NO: 4679

Sb06g018810

Sorghum

Polynucleotide
SEQ ID NO: 2551

bicolor

Polypeptide
SEQ ID NO: 2552

Genomic
SEQ ID NO: 4680

Sb06g018950

Sorghum

Polynucleotide
SEQ ID NO: 2553

bicolor

Polypeptide
SEQ ID NO: 2554

Genomic
SEQ ID NO: 4681

Sb06g019780

Sorghum

Polynucleotide
SEQ ID NO: 2555

bicolor

Polypeptide
SEQ ID NO: 2556

Genomic
SEQ ID NO: 4682

Sb06g020120

Sorghum

Polynucleotide
SEQ ID NO: 2557

bicolor

Polypeptide
SEQ ID NO: 2558

Genomic
SEQ ID NO: 4683

Sb06g020230

Sorghum

Polynucleotide
SEQ ID NO: 2559

bicolor

Polypeptide
SEQ ID NO: 2560

Genomic
SEQ ID NO: 4684

Sb06g020390

Sorghum

Polynucleotide
SEQ ID NO: 2561

bicolor

Polypeptide
SEQ ID NO: 2562

Genomic
SEQ ID NO: 4685

Sb06g020450

Sorghum

Polynucleotide
SEQ ID NO: 2563

bicolor

Polypeptide
SEQ ID NO: 2564

Genomic
SEQ ID NO: 4686

Sb06g020680

Sorghum

Polynucleotide
SEQ ID NO: 2565

bicolor

Polypeptide
SEQ ID NO: 2566

Genomic
SEQ ID NO: 4687

Sb06g021240

Sorghum

Polynucleotide
SEQ ID NO: 2567

bicolor

Polypeptide
SEQ ID NO: 2568

Genomic
SEQ ID NO: 4688

Sb06g022310

Sorghum

Polynucleotide
SEQ ID NO: 2569

bicolor

Polypeptide
SEQ ID NO: 2570

Genomic
SEQ ID NO: 4689

Sb06g022330

Sorghum

Polynucleotide
SEQ ID NO: 2571

bicolor

Polypeptide
SEQ ID NO: 2572

Genomic
SEQ ID NO: 4690

Sb06g022600

Sorghum

Polynucleotide
SEQ ID NO: 2573

bicolor

Polypeptide
SEQ ID NO: 2574

Genomic
SEQ ID NO: 4691

Sb06g022790

Sorghum

Polynucleotide
SEQ ID NO: 2575

bicolor

Polypeptide
SEQ ID NO: 2576

Genomic
SEQ ID NO: 4692

Sb06g022790

Sorghum

Polynucleotide
SEQ ID NO: 2577

bicolor

Polypeptide
SEQ ID NO: 2578

Genomic
SEQ ID NO: 4693

Sb06g022870

Sorghum

Polynucleotide
SEQ ID NO: 2579

bicolor

Polypeptide
SEQ ID NO: 2580

Genomic
SEQ ID NO: 4694

Sb06g136130

Sorghum

Polynucleotide
SEQ ID NO: 2581

bicolor

Polypeptide
SEQ ID NO: 2582

Genomic
SEQ ID NO: 4695

Sb06g023405

Sorghum

Polynucleotide
SEQ ID NO: 2583

bicolor

Polypeptide
SEQ ID NO: 2584

Genomic
SEQ ID NO: 4696

Sb06g136620

Sorghum

Polynucleotide
SEQ ID NO: 2585

bicolor

Polypeptide
SEQ ID NO: 2586

Genomic
SEQ ID NO: 4697

Sb06g023780

Sorghum

Polynucleotide
SEQ ID NO: 2587

bicolor

Polypeptide
SEQ ID NO: 2588

Genomic
SEQ ID NO: 4698

Sb06g024130

Sorghum

Polynucleotide
SEQ ID NO: 2589

bicolor

Polypeptide
SEQ ID NO: 2590

Genomic
SEQ ID NO: 4699

Sb06g137700

Sorghum

Polynucleotide
SEQ ID NO: 2591

bicolor

Polypeptide
SEQ ID NO: 2592

Genomic
SEQ ID NO: 4700

Sb06g024320

Sorghum

Polynucleotide
SEQ ID NO: 2593

bicolor

Polypeptide
SEQ ID NO: 2594

Genomic
SEQ ID NO: 4701

Sb06g024660

Sorghum

Polynucleotide
SEQ ID NO: 2595

bicolor

Polypeptide
SEQ ID NO: 2596

Genomic
SEQ ID NO: 4702

Sb06g025210

Sorghum

Polynucleotide
SEQ ID NO: 2597

bicolor

Polypeptide
SEQ ID NO: 2598

Genomic
SEQ ID NO: 4703

Sb06g025380

Sorghum

Polynucleotide
SEQ ID NO: 2599

bicolor

Polypeptide
SEQ ID NO: 2600

Genomic
SEQ ID NO: 4704

Sb06g025620

Sorghum

Polynucleotide
SEQ ID NO: 2601

bicolor

Polypeptide
SEQ ID NO: 2602

Genomic
SEQ ID NO: 4705

Sb06g139700

Sorghum

Polynucleotide
SEQ ID NO: 2603

bicolor

Polypeptide
SEQ ID NO: 2604

Genomic
SEQ ID NO: 4706

Sb06g025950

Sorghum

Polynucleotide
SEQ ID NO: 2605

bicolor

Polypeptide
SEQ ID NO: 2606

Genomic
SEQ ID NO: 4707

Sb06g026150

Sorghum

Polynucleotide
SEQ ID NO: 2607

bicolor

Polypeptide
SEQ ID NO: 2608

Genomic
SEQ ID NO: 4708

Sb06g026280

Sorghum

Polynucleotide
SEQ ID NO: 2609

bicolor

Polypeptide
SEQ ID NO: 2610

Genomic
SEQ ID NO: 4709

Sb06g026890

Sorghum

Polynucleotide
SEQ ID NO: 2611

bicolor

Polypeptide
SEQ ID NO: 2612

Genomic
SEQ ID NO: 4710

Sb06g027000

Sorghum

Polynucleotide
SEQ ID NO: 2613

bicolor

Polypeptide
SEQ ID NO: 2614

Genomic
SEQ ID NO: 4711

Sb06g027320

Sorghum

Polynucleotide
SEQ ID NO: 2615

bicolor

Polypeptide
SEQ ID NO: 2616

Genomic
SEQ ID NO: 4712

Sb06g027405

Sorghum

Polynucleotide
SEQ ID NO: 2617

bicolor

Polypeptide
SEQ ID NO: 2618

Genomic
SEQ ID NO: 4713

Sb06g027490

Sorghum

Polynucleotide
SEQ ID NO: 2619

bicolor

Polypeptide
SEQ ID NO: 2620

Genomic
SEQ ID NO: 4714

Sb06g027570

Sorghum

Polynucleotide
SEQ ID NO: 2621

bicolor

Polypeptide
SEQ ID NO: 2622

Genomic
SEQ ID NO: 4715

Sb06g027820

Sorghum

Polynucleotide
SEQ ID NO: 2623

bicolor

Polypeptide
SEQ ID NO: 2624

Genomic
SEQ ID NO: 4716

Sb06g028030

Sorghum

Polynucleotide
SEQ ID NO: 2625

bicolor

Polypeptide
SEQ ID NO: 2626

Genomic
SEQ ID NO: 4717

Sb06g028270

Sorghum

Polynucleotide
SEQ ID NO: 2627

bicolor

Polypeptide
SEQ ID NO: 2628

Genomic
SEQ ID NO: 4718

Sb06g028310

Sorghum

Polynucleotide
SEQ ID NO: 2629

bicolor

Polypeptide
SEQ ID NO: 2630

Genomic
SEQ ID NO: 4719

Sb06g028440

Sorghum

Polynucleotide
SEQ ID NO: 2631

bicolor

Polypeptide
SEQ ID NO: 2632

Genomic
SEQ ID NO: 4720

Sb06g028820

Sorghum

Polynucleotide
SEQ ID NO: 2633

bicolor

Polypeptide
SEQ ID NO: 2634

Genomic
SEQ ID NO: 4721

Sb06g028840

Sorghum

Polynucleotide
SEQ ID NO: 2635

bicolor

Polypeptide
SEQ ID NO: 2636

Genomic
SEQ ID NO: 4722

Sb06g028890

Sorghum

Polynucleotide
SEQ ID NO: 2637

bicolor

Polypeptide
SEQ ID NO: 2638

Genomic
SEQ ID NO: 4723

Sb06g029070

Sorghum

Polynucleotide
SEQ ID NO: 2639

bicolor

Polypeptide
SEQ ID NO: 2640

Genomic
SEQ ID NO: 4724

Sb06g029210

Sorghum

Polynucleotide
SEQ ID NO: 2641

bicolor

Polypeptide
SEQ ID NO: 2642

Genomic
SEQ ID NO: 4725

Sb06g029725

Sorghum

Polynucleotide
SEQ ID NO: 2643

bicolor

Polypeptide
SEQ ID NO: 2644

Genomic
SEQ ID NO: 4726

Sb06g030410

Sorghum

Polynucleotide
SEQ ID NO: 2645

bicolor

Polypeptide
SEQ ID NO: 2646

Genomic
SEQ ID NO: 4727

Sb06g030520

Sorghum

Polynucleotide
SEQ ID NO: 2647

bicolor

Polypeptide
SEQ ID NO: 2648

Genomic
SEQ ID NO: 4728

Sb06g030900

Sorghum

Polynucleotide
SEQ ID NO: 2649

bicolor

Polypeptide
SEQ ID NO: 2650

Genomic
SEQ ID NO: 4729

Sb06g030940

Sorghum

Polynucleotide
SEQ ID NO: 2651

bicolor

Polypeptide
SEQ ID NO: 2652

Genomic
SEQ ID NO: 4730

Sb06g031220

Sorghum

Polynucleotide
SEQ ID NO: 2653

bicolor

Polypeptide
SEQ ID NO: 2654

Genomic
SEQ ID NO: 4731

Sb06g031340

Sorghum

Polynucleotide
SEQ ID NO: 2655

bicolor

Polypeptide
SEQ ID NO: 2656

Genomic
SEQ ID NO: 4732

Sb06g032000

Sorghum

Polynucleotide
SEQ ID NO: 2657

bicolor

Polypeptide
SEQ ID NO: 2658

Genomic
SEQ ID NO: 4733

Sb06g148700

Sorghum

Polynucleotide
SEQ ID NO: 2659

bicolor

Polypeptide
SEQ ID NO: 2660

Genomic
SEQ ID NO: 4734

Sb06g032260

Sorghum

Polynucleotide
SEQ ID NO: 2661

bicolor

Polypeptide
SEQ ID NO: 2662

Genomic
SEQ ID NO: 4735

Sb06g032330

Sorghum

Polynucleotide
SEQ ID NO: 2663

bicolor

Polypeptide
SEQ ID NO: 2664

Genomic
SEQ ID NO: 4736

Sb06g032490

Sorghum

Polynucleotide
SEQ ID NO: 2665

bicolor

Polypeptide
SEQ ID NO: 2666

Genomic
SEQ ID NO: 4737

Sb06g032610

Sorghum

Polynucleotide
SEQ ID NO: 2667

bicolor

Polypeptide
SEQ ID NO: 2668

Genomic
SEQ ID NO: 4738

Sb06g032640

Sorghum

Polynucleotide
SEQ ID NO: 2669

bicolor

Polypeptide
SEQ ID NO: 2670

Genomic
SEQ ID NO: 4739

Sb06g032970

Sorghum

Polynucleotide
SEQ ID NO: 2671

bicolor

Polypeptide
SEQ ID NO: 2672

Genomic
SEQ ID NO: 4740

Sb06g033120

Sorghum

Polynucleotide
SEQ ID NO: 2673

bicolor

Polypeptide
SEQ ID NO: 2674

Genomic
SEQ ID NO: 4741

Sb06g150170

Sorghum

Polynucleotide
SEQ ID NO: 2675

bicolor

Polypeptide
SEQ ID NO: 2676

Genomic
SEQ ID NO: 4742

Sb06g033260

Sorghum

Polynucleotide
SEQ ID NO: 2677

bicolor

Polypeptide
SEQ ID NO: 2678

Genomic
SEQ ID NO: 4743

Sb06g033280

Sorghum

Polynucleotide
SEQ ID NO: 2679

bicolor

Polypeptide
SEQ ID NO: 2680

Genomic
SEQ ID NO: 4744

Sb06g033300

Sorghum

Polynucleotide
SEQ ID NO: 2681

bicolor

Polypeptide
SEQ ID NO: 2682

Genomic
SEQ ID NO: 4745

Sb06g033650

Sorghum

Polynucleotide
SEQ ID NO: 2683

bicolor

Polypeptide
SEQ ID NO: 2684

Genomic
SEQ ID NO: 4746

Sb06g033720

Sorghum

Polynucleotide
SEQ ID NO: 2685

bicolor

Polypeptide
SEQ ID NO: 2686

Genomic
SEQ ID NO: 4747

Sb06g034020

Sorghum

Polynucleotide
SEQ ID NO: 2687

bicolor

Polypeptide
SEQ ID NO: 2688

Genomic
SEQ ID NO: 4748

Sb06g034090

Sorghum

Polynucleotide
SEQ ID NO: 2689

bicolor

Polypeptide
SEQ ID NO: 2690

Genomic
SEQ ID NO: 4749

Sb06g034110

Sorghum

Polynucleotide
SEQ ID NO: 2691

bicolor

Polypeptide
SEQ ID NO: 2692

Genomic
SEQ ID NO: 4750

Sb06g034230

Sorghum

Polynucleotide
SEQ ID NO: 2693

bicolor

Polypeptide
SEQ ID NO: 2694

Genomic
SEQ ID NO: 4751

Sb07g000230

Sorghum

Polynucleotide
SEQ ID NO: 2695

bicolor

Polypeptide
SEQ ID NO: 2696

Genomic
SEQ ID NO: 4752

Sb07g000650

Sorghum

Polynucleotide
SEQ ID NO: 2697

bicolor

Polypeptide
SEQ ID NO: 2698

Genomic
SEQ ID NO: 4753

Sb07g000920

Sorghum

Polynucleotide
SEQ ID NO: 2699

bicolor

Polypeptide
SEQ ID NO: 2700

Genomic
SEQ ID NO: 4754

Sb07g001450

Sorghum

Polynucleotide
SEQ ID NO: 2701

bicolor

Polypeptide
SEQ ID NO: 2702

Genomic
SEQ ID NO: 4755

Sb07g001580

Sorghum

Polynucleotide
SEQ ID NO: 2703

bicolor

Polypeptide
SEQ ID NO: 2704

Genomic
SEQ ID NO: 4756

Sb07g002500

Sorghum

Polynucleotide
SEQ ID NO: 2705

bicolor

Polypeptide
SEQ ID NO: 2706

Genomic
SEQ ID NO: 4757

Sb07g002650

Sorghum

Polynucleotide
SEQ ID NO: 2707

bicolor

Polypeptide
SEQ ID NO: 2708

Genomic
SEQ ID NO: 4758

Sb07g002900

Sorghum

Polynucleotide
SEQ ID NO: 2709

bicolor

Polypeptide
SEQ ID NO: 2710

Genomic
SEQ ID NO: 4759

Sb07g003190

Sorghum

Polynucleotide
SEQ ID NO: 2711

bicolor

Polypeptide
SEQ ID NO: 2712

Genomic
SEQ ID NO: 4760

Sb07g003280

Sorghum

Polynucleotide
SEQ ID NO: 2713

bicolor

Polypeptide
SEQ ID NO: 2714

Genomic
SEQ ID NO: 4761

Sb07g003510

Sorghum

Polynucleotide
SEQ ID NO: 2715

bicolor

Polypeptide
SEQ ID NO: 2716

Genomic
SEQ ID NO: 4762

Sb07g003590

Sorghum

Polynucleotide
SEQ ID NO: 2717

bicolor

Polypeptide
SEQ ID NO: 2718

Genomic
SEQ ID NO: 4763

Sb07g003600

Sorghum

Polynucleotide
SEQ ID NO: 2719

bicolor

Polypeptide
SEQ ID NO: 2720

Genomic
SEQ ID NO: 4764

Sb07g003650

Sorghum

Polynucleotide
SEQ ID NO: 2721

bicolor

Polypeptide
SEQ ID NO: 2722

Genomic
SEQ ID NO: 4765

Sb07g004260

Sorghum

Polynucleotide
SEQ ID NO: 2723

bicolor

Polypeptide
SEQ ID NO: 2724

Genomic
SEQ ID NO: 4766

Sb07g004700

Sorghum

Polynucleotide
SEQ ID NO: 2725

bicolor

Polypeptide
SEQ ID NO: 2726

Genomic
SEQ ID NO: 4767

Sb07g014030

Sorghum

Polynucleotide
SEQ ID NO: 2727

bicolor

Polypeptide
SEQ ID NO: 2728

Genomic
SEQ ID NO: 4768

Sb07g005470

Sorghum

Polynucleotide
SEQ ID NO: 2729

bicolor

Polypeptide
SEQ ID NO: 2730

Genomic
SEQ ID NO: 4769

Sb07g005500

Sorghum

Polynucleotide
SEQ ID NO: 2731

bicolor

Polypeptide
SEQ ID NO: 2732

Genomic
SEQ ID NO: 4770

Sb07g005660

Sorghum

Polynucleotide
SEQ ID NO: 2733

bicolor

Polypeptide
SEQ ID NO: 2734

Genomic
SEQ ID NO: 4771

Sb07g005685

Sorghum

Polynucleotide
SEQ ID NO: 2735

bicolor

Polypeptide
SEQ ID NO: 2736

Genomic
SEQ ID NO: 4772

Sb07g006220

Sorghum

Polynucleotide
SEQ ID NO: 2737

bicolor

Polypeptide
SEQ ID NO: 2738

Genomic
SEQ ID NO: 4773

Sb07g006300

Sorghum

Polynucleotide
SEQ ID NO: 2739

bicolor

Polypeptide
SEQ ID NO: 2740

Genomic
SEQ ID NO: 4774

Sb07g006390

Sorghum

Polynucleotide
SEQ ID NO: 2741

bicolor

Polypeptide
SEQ ID NO: 2742

Genomic
SEQ ID NO: 4775

Sb07g019850

Sorghum

Polynucleotide
SEQ ID NO: 2743

bicolor

Polypeptide
SEQ ID NO: 2744

Genomic
SEQ ID NO: 4776

Sb07g009450

Sorghum

Polynucleotide
SEQ ID NO: 2745

bicolor

Polypeptide
SEQ ID NO: 2746

Genomic
SEQ ID NO: 4777

Sb07g009560

Sorghum

Polynucleotide
SEQ ID NO: 2747

bicolor

Polypeptide
SEQ ID NO: 2748

Genomic
SEQ ID NO: 4778

Sb07g009570

Sorghum

Polynucleotide
SEQ ID NO: 2749

bicolor

Polypeptide
SEQ ID NO: 2750

Genomic
SEQ ID NO: 4779

Sb07g010440

Sorghum

Polynucleotide
SEQ ID NO: 2751

bicolor

Polypeptide
SEQ ID NO: 2752

Genomic
SEQ ID NO: 4780

Sb07g011460

Sorghum

Polynucleotide
SEQ ID NO: 2753

bicolor

Polypeptide
SEQ ID NO: 2754

Genomic
SEQ ID NO: 4781

Sb07g012110

Sorghum

Polynucleotide
SEQ ID NO: 2755

bicolor

Polypeptide
SEQ ID NO: 2756

Genomic
SEQ ID NO: 4782

Sb07g014210

Sorghum

Polynucleotide
SEQ ID NO: 2757

bicolor

Polypeptide
SEQ ID NO: 2758

Genomic
SEQ ID NO: 4783

Sb07g082870

Sorghum

Polynucleotide
SEQ ID NO: 2759

bicolor

Polypeptide
SEQ ID NO: 2760

Genomic
SEQ ID NO: 4784

Sb07g015150

Sorghum

Polynucleotide
SEQ ID NO: 2761

bicolor

Polypeptide
SEQ ID NO: 2762

Genomic
SEQ ID NO: 4785

Sb07g015160

Sorghum

Polynucleotide
SEQ ID NO: 2763

bicolor

Polypeptide
SEQ ID NO: 2764

Genomic
SEQ ID NO: 4786

Sb07g015390

Sorghum

Polynucleotide
SEQ ID NO: 2765

bicolor

Polypeptide
SEQ ID NO: 2766

Genomic
SEQ ID NO: 4787

Sb07g018840

Sorghum

Polynucleotide
SEQ ID NO: 2767

bicolor

Polypeptide
SEQ ID NO: 2768

Genomic
SEQ ID NO: 4788

Sb07g019180

Sorghum

Polynucleotide
SEQ ID NO: 2769

bicolor

Polypeptide
SEQ ID NO: 2770

Genomic
SEQ ID NO: 4789

Sb07g019220

Sorghum

Polynucleotide
SEQ ID NO: 2771

bicolor

Polypeptide
SEQ ID NO: 2772

Genomic
SEQ ID NO: 4790

Sb07g019450

Sorghum

Polynucleotide
SEQ ID NO: 2773

bicolor

Polypeptide
SEQ ID NO: 2774

Genomic
SEQ ID NO: 4791

Sb07g019470

Sorghum

Polynucleotide
SEQ ID NO: 2775

bicolor

Polypeptide
SEQ ID NO: 2776

Genomic
SEQ ID NO: 4792

Sb07g019750

Sorghum

Polynucleotide
SEQ ID NO: 2777

bicolor

Polypeptide
SEQ ID NO: 2778

Genomic
SEQ ID NO: 4793

Sb07g019840

Sorghum

Polynucleotide
SEQ ID NO: 2779

bicolor

Polypeptide
SEQ ID NO: 2780

Genomic
SEQ ID NO: 4794

Sb07g019863

Sorghum

Polynucleotide
SEQ ID NO: 2781

bicolor

Polypeptide
SEQ ID NO: 2782

Genomic
SEQ ID NO: 4795

Sb07g020220

Sorghum

Polynucleotide
SEQ ID NO: 2783

bicolor

Polypeptide
SEQ ID NO: 2784

Genomic
SEQ ID NO: 4796

Sb07g020640

Sorghum

Polynucleotide
SEQ ID NO: 2785

bicolor

Polypeptide
SEQ ID NO: 2786

Genomic
SEQ ID NO: 4797

Sb07g020940

Sorghum

Polynucleotide
SEQ ID NO: 2787

bicolor

Polypeptide
SEQ ID NO: 2788

Genomic
SEQ ID NO: 4798

Sb07g021060

Sorghum

Polynucleotide
SEQ ID NO: 2789

bicolor

Polypeptide
SEQ ID NO: 2790

Genomic
SEQ ID NO: 4799

Sb07g021100

Sorghum

Polynucleotide
SEQ ID NO: 2791

bicolor

Polypeptide
SEQ ID NO: 2792

Genomic
SEQ ID NO: 4800

Sb07g021140

Sorghum

Polynucleotide
SEQ ID NO: 2793

bicolor

Polypeptide
SEQ ID NO: 2794

Genomic
SEQ ID NO: 4801

Sb07g021160

Sorghum

Polynucleotide
SEQ ID NO: 2795

bicolor

Polypeptide
SEQ ID NO: 2796

Genomic
SEQ ID NO: 4802

Sb07g021350

Sorghum

Polynucleotide
SEQ ID NO: 2797

bicolor

Polypeptide
SEQ ID NO: 2798

Genomic
SEQ ID NO: 4803

Sb07g021400

Sorghum

Polynucleotide
SEQ ID NO: 2799

bicolor

Polypeptide
SEQ ID NO: 2800

Genomic
SEQ ID NO: 4804

Sb07g021630

Sorghum

Polynucleotide
SEQ ID NO: 2801

bicolor

Polypeptide
SEQ ID NO: 2802

Genomic
SEQ ID NO: 4805

Sb07g021700

Sorghum

Polynucleotide
SEQ ID NO: 2803

bicolor

Polypeptide
SEQ ID NO: 2804

Genomic
SEQ ID NO: 4806

Sb07g022000

Sorghum

Polynucleotide
SEQ ID NO: 2805

bicolor

Polypeptide
SEQ ID NO: 2806

Genomic
SEQ ID NO: 4807

Sb07g022480

Sorghum

Polynucleotide
SEQ ID NO: 2807

bicolor

Polypeptide
SEQ ID NO: 2808

Genomic
SEQ ID NO: 4808

Sb07g144470

Sorghum

Polynucleotide
SEQ ID NO: 2809

bicolor

Polypeptide
SEQ ID NO: 2810

Genomic
SEQ ID NO: 4809

Sb07g023740

Sorghum

Polynucleotide
SEQ ID NO: 2811

bicolor

Polypeptide
SEQ ID NO: 2812

Genomic
SEQ ID NO: 4810

Sb07g023950

Sorghum

Polynucleotide
SEQ ID NO: 2813

bicolor

Polypeptide
SEQ ID NO: 2814

Genomic
SEQ ID NO: 4811

Sb07g024150

Sorghum

Polynucleotide
SEQ ID NO: 2815

bicolor

Polypeptide
SEQ ID NO: 2816

Genomic
SEQ ID NO: 4812

Sb07g024450

Sorghum

Polynucleotide
SEQ ID NO: 2817

bicolor

Polypeptide
SEQ ID NO: 2818

Genomic
SEQ ID NO: 4813

Sb07g024460

Sorghum

Polynucleotide
SEQ ID NO: 2819

bicolor

Polypeptide
SEQ ID NO: 2820

Genomic
SEQ ID NO: 4814

Sb07g024490

Sorghum

Polynucleotide
SEQ ID NO: 2821

bicolor

Polypeptide
SEQ ID NO: 2822

Genomic
SEQ ID NO: 4815

Sb07g024860

Sorghum

Polynucleotide
SEQ ID NO: 2823

bicolor

Polypeptide
SEQ ID NO: 2824

Genomic
SEQ ID NO: 4816

Sb07g025470

Sorghum

Polynucleotide
SEQ ID NO: 2825

bicolor

Polypeptide
SEQ ID NO: 2826

Genomic
SEQ ID NO: 4817

Sb07g025510

Sorghum

Polynucleotide
SEQ ID NO: 2827

bicolor

Polypeptide
SEQ ID NO: 2828

Genomic
SEQ ID NO: 4818

Sb07g026000

Sorghum

Polynucleotide
SEQ ID NO: 2829

bicolor

Polypeptide
SEQ ID NO: 2830

Genomic
SEQ ID NO: 4819

Sb07g026260

Sorghum

Polynucleotide
SEQ ID NO: 2831

bicolor

Polypeptide
SEQ ID NO: 2832

Genomic
SEQ ID NO: 4820

Sb07g026480

Sorghum

Polynucleotide
SEQ ID NO: 2833

bicolor

Polypeptide
SEQ ID NO: 2834

Genomic
SEQ ID NO: 4821

Sb07g027290

Sorghum

Polynucleotide
SEQ ID NO: 2835

bicolor

Polypeptide
SEQ ID NO: 2836

Genomic
SEQ ID NO: 4822

Sb07g027510

Sorghum

Polynucleotide
SEQ ID NO: 2837

bicolor

Polypeptide
SEQ ID NO: 2838

Genomic
SEQ ID NO: 4823

Sb07g027570

Sorghum

Polynucleotide
SEQ ID NO: 2839

bicolor

Polypeptide
SEQ ID NO: 2840

Genomic
SEQ ID NO: 4824

Sb07g027640

Sorghum

Polynucleotide
SEQ ID NO: 2841

bicolor

Polypeptide
SEQ ID NO: 2842

Genomic
SEQ ID NO: 4825

Sb07g027650

Sorghum

Polynucleotide
SEQ ID NO: 2843

bicolor

Polypeptide
SEQ ID NO: 2844

Genomic
SEQ ID NO: 4826

Sb07g027830

Sorghum

Polynucleotide
SEQ ID NO: 2845

bicolor

Polypeptide
SEQ ID NO: 2846

Genomic
SEQ ID NO: 4827

Sb07g027950

Sorghum

Polynucleotide
SEQ ID NO: 2847

bicolor

Polypeptide
SEQ ID NO: 2848

Genomic
SEQ ID NO: 4828

Sb07g028140

Sorghum

Polynucleotide
SEQ ID NO: 2849

bicolor

Polypeptide
SEQ ID NO: 2850

Genomic
SEQ ID NO: 4829

Sb07g028200

Sorghum

Polynucleotide
SEQ ID NO: 2851

bicolor

Polypeptide
SEQ ID NO: 2852

Genomic
SEQ ID NO: 4830

Sb07g028980

Sorghum

Polynucleotide
SEQ ID NO: 2853

bicolor

Polypeptide
SEQ ID NO: 2854

Genomic
SEQ ID NO: 4831

Sb07g029190

Sorghum

Polynucleotide
SEQ ID NO: 2855

bicolor

Polypeptide
SEQ ID NO: 2856

Genomic
SEQ ID NO: 4832

Sb08g000370

Sorghum

Polynucleotide
SEQ ID NO: 2857

bicolor

Polypeptide
SEQ ID NO: 2858

Genomic
SEQ ID NO: 4833

Sb08g000640

Sorghum

Polynucleotide
SEQ ID NO: 2859

bicolor

Polypeptide
SEQ ID NO: 2860

Genomic
SEQ ID NO: 4834

Sb08g001050

Sorghum

Polynucleotide
SEQ ID NO: 2861

bicolor

Polypeptide
SEQ ID NO: 2862

Genomic
SEQ ID NO: 4835

Sb08g001340

Sorghum

Polynucleotide
SEQ ID NO: 2863

bicolor

Polypeptide
SEQ ID NO: 2864

Genomic
SEQ ID NO: 4836

Sb08g001730

Sorghum

Polynucleotide
SEQ ID NO: 2865

bicolor

Polypeptide
SEQ ID NO: 2866

Genomic
SEQ ID NO: 4837

Sb08g001930

Sorghum

Polynucleotide
SEQ ID NO: 2867

bicolor

Polypeptide
SEQ ID NO: 2868

Genomic
SEQ ID NO: 4838

Sb08g002000

Sorghum

Polynucleotide
SEQ ID NO: 2869

bicolor

Polypeptide
SEQ ID NO: 2870

Genomic
SEQ ID NO: 4839

Sb08g002056

Sorghum

Polynucleotide
SEQ ID NO: 2871

bicolor

Polypeptide
SEQ ID NO: 2872

Genomic
SEQ ID NO: 4840

Sb08g002240

Sorghum

Polynucleotide
SEQ ID NO: 2873

bicolor

Polypeptide
SEQ ID NO: 2874

Genomic
SEQ ID NO: 4841

Sb08g002430

Sorghum

Polynucleotide
SEQ ID NO: 2875

bicolor

Polypeptide
SEQ ID NO: 2876

Genomic
SEQ ID NO: 4842

Sb08g002707

Sorghum

Polynucleotide
SEQ ID NO: 2877

bicolor

Polypeptide
SEQ ID NO: 2878

Genomic
SEQ ID NO: 4843

Sb08g002720

Sorghum

Polynucleotide
SEQ ID NO: 2879

bicolor

Polypeptide
SEQ ID NO: 2880

Genomic
SEQ ID NO: 4844

Sb08g002910

Sorghum

Polynucleotide
SEQ ID NO: 2881

bicolor

Polypeptide
SEQ ID NO: 2882

Genomic
SEQ ID NO: 4845

Sb08g002960

Sorghum

Polynucleotide
SEQ ID NO: 2883

bicolor

Polypeptide
SEQ ID NO: 2884

Genomic
SEQ ID NO: 4846

Sb08g003430

Sorghum

Polynucleotide
SEQ ID NO: 2885

bicolor

Polypeptide
SEQ ID NO: 2886

Genomic
SEQ ID NO: 4847

Sb08g004050

Sorghum

Polynucleotide
SEQ ID NO: 2887

bicolor

Polypeptide
SEQ ID NO: 2888

Genomic
SEQ ID NO: 4848

Sb08g004180

Sorghum

Polynucleotide
SEQ ID NO: 2889

bicolor

Polypeptide
SEQ ID NO: 2890

Genomic
SEQ ID NO: 4849

Sb08g004190

Sorghum

Polynucleotide
SEQ ID NO: 2891

bicolor

Polypeptide
SEQ ID NO: 2892

Genomic
SEQ ID NO: 4850

Sb08g005260

Sorghum

Polynucleotide
SEQ ID NO: 2893

bicolor

Polypeptide
SEQ ID NO: 2894

Genomic
SEQ ID NO: 4851

Sb08g005300

Sorghum

Polynucleotide
SEQ ID NO: 2895

bicolor

Polypeptide
SEQ ID NO: 2896

Genomic
SEQ ID NO: 4852

Sb08g005340

Sorghum

Polynucleotide
SEQ ID NO: 2897

bicolor

Polypeptide
SEQ ID NO: 2898

Genomic
SEQ ID NO: 4853

Sb08g016200

Sorghum

Polynucleotide
SEQ ID NO: 2899

bicolor

Polypeptide
SEQ ID NO: 2900

Genomic
SEQ ID NO: 4854

Sb08g006180

Sorghum

Polynucleotide
SEQ ID NO: 2901

bicolor

Polypeptide
SEQ ID NO: 2902

Genomic
SEQ ID NO: 4855

Sb08g006460

Sorghum

Polynucleotide
SEQ ID NO: 2903

bicolor

Polypeptide
SEQ ID NO: 2904

Genomic
SEQ ID NO: 4856

Sb08g006540

Sorghum

Polynucleotide
SEQ ID NO: 2905

bicolor

Polypeptide
SEQ ID NO: 2906

Genomic
SEQ ID NO: 4857

Sb08g006600

Sorghum

Polynucleotide
SEQ ID NO: 2907

bicolor

Polypeptide
SEQ ID NO: 2908

Genomic
SEQ ID NO: 4858

Sb08g006690

Sorghum

Polynucleotide
SEQ ID NO: 2909

bicolor

Polypeptide
SEQ ID NO: 2910

Genomic
SEQ ID NO: 4859

Sb08g007300

Sorghum

Polynucleotide
SEQ ID NO: 2911

bicolor

Polypeptide
SEQ ID NO: 2912

Genomic
SEQ ID NO: 4860

Sb08g007570

Sorghum

Polynucleotide
SEQ ID NO: 2913

bicolor

Polypeptide
SEQ ID NO: 2914

Genomic
SEQ ID NO: 4861

Sb08g008180

Sorghum

Polynucleotide
SEQ ID NO: 2915

bicolor

Polypeptide
SEQ ID NO: 2916

Genomic
SEQ ID NO: 4862

Sb08g039210

Sorghum

Polynucleotide
SEQ ID NO: 2917

bicolor

Polypeptide
SEQ ID NO: 2918

Genomic
SEQ ID NO: 4863

Sb08g008505

Sorghum

Polynucleotide
SEQ ID NO: 2919

bicolor

Polypeptide
SEQ ID NO: 2920

Genomic
SEQ ID NO: 4864

Sb08g009100

Sorghum

Polynucleotide
SEQ ID NO: 2921

bicolor

Polypeptide
SEQ ID NO: 2922

Genomic
SEQ ID NO: 4865

Sb08g011300

Sorghum

Polynucleotide
SEQ ID NO: 2923

bicolor

Polypeptide
SEQ ID NO: 2924

Genomic
SEQ ID NO: 4866

Sb08g012560

Sorghum

Polynucleotide
SEQ ID NO: 2925

bicolor

Polypeptide
SEQ ID NO: 2926

Genomic
SEQ ID NO: 4867

Sb08g015000

Sorghum

Polynucleotide
SEQ ID NO: 2927

bicolor

Polypeptide
SEQ ID NO: 2928

Genomic
SEQ ID NO: 4868

Sb08g015131

Sorghum

Polynucleotide
SEQ ID NO: 2929

bicolor

Polypeptide
SEQ ID NO: 2930

Genomic
SEQ ID NO: 4869

Sb08g015555

Sorghum

Polynucleotide
SEQ ID NO: 2931

bicolor

Polypeptide
SEQ ID NO: 2932

Genomic
SEQ ID NO: 4870

Sb08g016370

Sorghum

Polynucleotide
SEQ ID NO: 2933

bicolor

Polypeptide
SEQ ID NO: 2934

Genomic
SEQ ID NO: 4871

Sb08g016490

Sorghum

Polynucleotide
SEQ ID NO: 2935

bicolor

Polypeptide
SEQ ID NO: 2936

Genomic
SEQ ID NO: 4872

Sb08g016720

Sorghum

Polynucleotide
SEQ ID NO: 2937

bicolor

Polypeptide
SEQ ID NO: 2938

Genomic
SEQ ID NO: 4873

Sb08g017180

Sorghum

Polynucleotide
SEQ ID NO: 2939

bicolor

Polypeptide
SEQ ID NO: 2940

Genomic
SEQ ID NO: 4874

Sb08g017210

Sorghum

Polynucleotide
SEQ ID NO: 2941

bicolor

Polypeptide
SEQ ID NO: 2942

Genomic
SEQ ID NO: 4875

Sb08g017700

Sorghum

Polynucleotide
SEQ ID NO: 2943

bicolor

Polypeptide
SEQ ID NO: 2944

Genomic
SEQ ID NO: 4876

Sb08g017830

Sorghum

Polynucleotide
SEQ ID NO: 2945

bicolor

Polypeptide
SEQ ID NO: 2946

Genomic
SEQ ID NO: 4877

Sb08g018160

Sorghum

Polynucleotide
SEQ ID NO: 2947

bicolor

Polypeptide
SEQ ID NO: 2948

Genomic
SEQ ID NO: 4878

Sb08g117320

Sorghum

Polynucleotide
SEQ ID NO: 2949

bicolor

Polypeptide
SEQ ID NO: 2950

Genomic
SEQ ID NO: 4879

Sb08g018493

Sorghum

Polynucleotide
SEQ ID NO: 2951

bicolor

Polypeptide
SEQ ID NO: 2952

Genomic
SEQ ID NO: 4880

Sb08g018740

Sorghum

Polynucleotide
SEQ ID NO: 2953

bicolor

Polypeptide
SEQ ID NO: 2954

Genomic
SEQ ID NO: 4881

Sb08g018890

Sorghum

Polynucleotide
SEQ ID NO: 2955

bicolor

Polypeptide
SEQ ID NO: 2956

Genomic
SEQ ID NO: 4882

Sb08g120510

Sorghum

Polynucleotide
SEQ ID NO: 2957

bicolor

Polypeptide
SEQ ID NO: 2958

Genomic
SEQ ID NO: 4883

Sb08g020750

Sorghum

Polynucleotide
SEQ ID NO: 2959

bicolor

Polypeptide
SEQ ID NO: 2960

Genomic
SEQ ID NO: 4884

Sb08g020830

Sorghum

Polynucleotide
SEQ ID NO: 2961

bicolor

Polypeptide
SEQ ID NO: 2962

Genomic
SEQ ID NO: 4885

Sb08g020910

Sorghum

Polynucleotide
SEQ ID NO: 2963

bicolor

Polypeptide
SEQ ID NO: 2964

Genomic
SEQ ID NO: 4886

Sb08g021630

Sorghum

Polynucleotide
SEQ ID NO: 2965

bicolor

Polypeptide
SEQ ID NO: 2966

Genomic
SEQ ID NO: 4887

Sb08g021670

Sorghum

Polynucleotide
SEQ ID NO: 2967

bicolor

Polypeptide
SEQ ID NO: 2968

Genomic
SEQ ID NO: 4888

Sb08g022230

Sorghum

Polynucleotide
SEQ ID NO: 2969

bicolor

Polypeptide
SEQ ID NO: 2970

Genomic
SEQ ID NO: 4889

Sb08g022270

Sorghum

Polynucleotide
SEQ ID NO: 2971

bicolor

Polypeptide
SEQ ID NO: 2972

Genomic
SEQ ID NO: 4890

Sb08g022390

Sorghum

Polynucleotide
SEQ ID NO: 2973

bicolor

Polypeptide
SEQ ID NO: 2974

Genomic
SEQ ID NO: 4891

Sb08g020000

Sorghum

Polynucleotide
SEQ ID NO: 2975

bicolor

Polypeptide
SEQ ID NO: 2976

Genomic
SEQ ID NO: 4892

Sb08g022830

Sorghum

Polynucleotide
SEQ ID NO: 2977

bicolor

Polypeptide
SEQ ID NO: 2978

Genomic
SEQ ID NO: 4893

Sb08g023040

Sorghum

Polynucleotide
SEQ ID NO: 2979

bicolor

Polypeptide
SEQ ID NO: 2980

Genomic
SEQ ID NO: 4894

Sb09g000280

Sorghum

Polynucleotide
SEQ ID NO: 2981

bicolor

Polypeptide
SEQ ID NO: 2982

Genomic
SEQ ID NO: 4895

Sb09g000330

Sorghum

Polynucleotide
SEQ ID NO: 2983

bicolor

Polypeptide
SEQ ID NO: 2984

Genomic
SEQ ID NO: 4896

Sb09g000350

Sorghum

Polynucleotide
SEQ ID NO: 2985

bicolor

Polypeptide
SEQ ID NO: 2986

Genomic
SEQ ID NO: 4897

Sb09g000780

Sorghum

Polynucleotide
SEQ ID NO: 2987

bicolor

Polypeptide
SEQ ID NO: 2988

Genomic
SEQ ID NO: 4898

Sb09g000970

Sorghum

Polynucleotide
SEQ ID NO: 2989

bicolor

Polypeptide
SEQ ID NO: 2990

Genomic
SEQ ID NO: 4899

Sb09g001080

Sorghum

Polynucleotide
SEQ ID NO: 2991

bicolor

Polypeptide
SEQ ID NO: 2992

Genomic
SEQ ID NO: 4900

Sb09g001430

Sorghum

Polynucleotide
SEQ ID NO: 2993

bicolor

Polypeptide
SEQ ID NO: 2994

Genomic
SEQ ID NO: 4901

Sb09g001530

Sorghum

Polynucleotide
SEQ ID NO: 2995

bicolor

Polypeptide
SEQ ID NO: 2996

Genomic
SEQ ID NO: 4902

Sb09g001880

Sorghum

Polynucleotide
SEQ ID NO: 2997

bicolor

Polypeptide
SEQ ID NO: 2998

Genomic
SEQ ID NO: 4903

Sb09g002250

Sorghum

Polynucleotide
SEQ ID NO: 2999

bicolor

Polypeptide
SEQ ID NO: 3000

Genomic
SEQ ID NO: 4904

Sb09g002400

Sorghum

Polynucleotide
SEQ ID NO: 3001

bicolor

Polypeptide
SEQ ID NO: 3002

Genomic
SEQ ID NO: 4905

Sb09g002860

Sorghum

Polynucleotide
SEQ ID NO: 3003

bicolor

Polypeptide
SEQ ID NO: 3004

Genomic
SEQ ID NO: 4906

Sb09g003060

Sorghum

Polynucleotide
SEQ ID NO: 3005

bicolor

Polypeptide
SEQ ID NO: 3006

Genomic
SEQ ID NO: 4907

Sb09g003630

Sorghum

Polynucleotide
SEQ ID NO: 3007

bicolor

Polypeptide
SEQ ID NO: 3008

Genomic
SEQ ID NO: 4908

Sb09g004000

Sorghum

Polynucleotide
SEQ ID NO: 3009

bicolor

Polypeptide
SEQ ID NO: 3010

Genomic
SEQ ID NO: 4909

Sb09g004150

Sorghum

Polynucleotide
SEQ ID NO: 3011

bicolor

Polypeptide
SEQ ID NO: 3012

Genomic
SEQ ID NO: 4910

Sb09g004430

Sorghum

Polynucleotide
SEQ ID NO: 3013

bicolor

Polypeptide
SEQ ID NO: 3014

Genomic
SEQ ID NO: 4911

Sb09g004490

Sorghum

Polynucleotide
SEQ ID NO: 3015

bicolor

Polypeptide
SEQ ID NO: 3016

Genomic
SEQ ID NO: 4912

Sb09g004520

Sorghum

Polynucleotide
SEQ ID NO: 3017

bicolor

Polypeptide
SEQ ID NO: 3018

Genomic
SEQ ID NO: 4913

Sb09g004630

Sorghum

Polynucleotide
SEQ ID NO: 3019

bicolor

Polypeptide
SEQ ID NO: 3020

Genomic
SEQ ID NO: 4914

Sb09g004685

Sorghum

Polynucleotide
SEQ ID NO: 3021

bicolor

Polypeptide
SEQ ID NO: 3022

Genomic
SEQ ID NO: 4915

Sb09g004883

Sorghum

Polynucleotide
SEQ ID NO: 3023

bicolor

Polypeptide
SEQ ID NO: 3024

Genomic
SEQ ID NO: 4916

Sb09g005070

Sorghum

Polynucleotide
SEQ ID NO: 3025

bicolor

Polypeptide
SEQ ID NO: 3026

Genomic
SEQ ID NO: 4917

Sb09g005250

Sorghum

Polynucleotide
SEQ ID NO: 3027

bicolor

Polypeptide
SEQ ID NO: 3028

Genomic
SEQ ID NO: 4918

Sb09g005380

Sorghum

Polynucleotide
SEQ ID NO: 3029

bicolor

Polypeptide
SEQ ID NO: 3030

Genomic
SEQ ID NO: 4919

Sb09g005450

Sorghum

Polynucleotide
SEQ ID NO: 3031

bicolor

Polypeptide
SEQ ID NO: 3032

Genomic
SEQ ID NO: 4920

Sb09g005650

Sorghum

Polynucleotide
SEQ ID NO: 3033

bicolor

Polypeptide
SEQ ID NO: 3034

Genomic
SEQ ID NO: 4921

Sb09g006040

Sorghum

Polynucleotide
SEQ ID NO: 3035

bicolor

Polypeptide
SEQ ID NO: 3036

Genomic
SEQ ID NO: 4922

Sb09g006090

Sorghum

Polynucleotide
SEQ ID NO: 3037

bicolor

Polypeptide
SEQ ID NO: 3038

Genomic
SEQ ID NO: 4923

Sb09g006900

Sorghum

Polynucleotide
SEQ ID NO: 3039

bicolor

Polypeptide
SEQ ID NO: 3040

Genomic
SEQ ID NO: 4924

Sb09g007185

Sorghum

Polynucleotide
SEQ ID NO: 3041

bicolor

Polypeptide
SEQ ID NO: 3042

Genomic
SEQ ID NO: 4925

Sb09g008070

Sorghum

Polynucleotide
SEQ ID NO: 3043

bicolor

Polypeptide
SEQ ID NO: 3044

Genomic
SEQ ID NO: 4926

Sb09g065360

Sorghum

Polynucleotide
SEQ ID NO: 3045

bicolor

Polypeptide
SEQ ID NO: 3046

Genomic
SEQ ID NO: 4927

Sb09g016510

Sorghum

Polynucleotide
SEQ ID NO: 3047

bicolor

Polypeptide
SEQ ID NO: 3048

Genomic
SEQ ID NO: 4928

Sb09g126280

Sorghum

Polynucleotide
SEQ ID NO: 3049

bicolor

Polypeptide
SEQ ID NO: 3050

Genomic
SEQ ID NO: 4929

Sb09g018720

Sorghum

Polynucleotide
SEQ ID NO: 3051

bicolor

Polypeptide
SEQ ID NO: 3052

Genomic
SEQ ID NO: 4930

Sb09g019100

Sorghum

Polynucleotide
SEQ ID NO: 3053

bicolor

Polypeptide
SEQ ID NO: 3054

Genomic
SEQ ID NO: 4931

Sb09g019240

Sorghum

Polynucleotide
SEQ ID NO: 3055

bicolor

Polypeptide
SEQ ID NO: 3056

Genomic
SEQ ID NO: 4932

Sb09g019290

Sorghum

Polynucleotide
SEQ ID NO: 3057

bicolor

Polypeptide
SEQ ID NO: 3058

Genomic
SEQ ID NO: 4933

Sb09g019590

Sorghum

Polynucleotide
SEQ ID NO: 3059

bicolor

Polypeptide
SEQ ID NO: 3060

Genomic
SEQ ID NO: 4934

Sb09g130560

Sorghum

Polynucleotide
SEQ ID NO: 3061

bicolor

Polypeptide
SEQ ID NO: 3062

Genomic
SEQ ID NO: 4935

Sb09g019680

Sorghum

Polynucleotide
SEQ ID NO: 3063

bicolor

Polypeptide
SEQ ID NO: 3064

Genomic
SEQ ID NO: 4936

Sb09g019760

Sorghum

Polynucleotide
SEQ ID NO: 3065

bicolor

Polypeptide
SEQ ID NO: 3066

Genomic
SEQ ID NO: 4937

Sb09g019940

Sorghum

Polynucleotide
SEQ ID NO: 3067

bicolor

Polypeptide
SEQ ID NO: 3068

Genomic
SEQ ID NO: 4938

Sb09g020070

Sorghum

Polynucleotide
SEQ ID NO: 3069

bicolor

Polypeptide
SEQ ID NO: 3070

Genomic
SEQ ID NO: 4939

Sb09g132690

Sorghum

Polynucleotide
SEQ ID NO: 3071

bicolor

Polypeptide
SEQ ID NO: 3072

Genomic
SEQ ID NO: 4940

Sb09g020410

Sorghum

Polynucleotide
SEQ ID NO: 3073

bicolor

Polypeptide
SEQ ID NO: 3074

Genomic
SEQ ID NO: 4941

Sb09g020820

Sorghum

Polynucleotide
SEQ ID NO: 3075

bicolor

Polypeptide
SEQ ID NO: 3076

Genomic
SEQ ID NO: 4942

Sb09g020830

Sorghum

Polynucleotide
SEQ ID NO: 3077

bicolor

Polypeptide
SEQ ID NO: 3078

Genomic
SEQ ID NO: 4943

Sb09g020860

Sorghum

Polynucleotide
SEQ ID NO: 3079

bicolor

Polypeptide
SEQ ID NO: 3080

Genomic
SEQ ID NO: 4944

Sb09g133620

Sorghum

Polynucleotide
SEQ ID NO: 3081

bicolor

Polypeptide
SEQ ID NO: 3082

Genomic
SEQ ID NO: 4945

Sb09g020940

Sorghum

Polynucleotide
SEQ ID NO: 3083

bicolor

Polypeptide
SEQ ID NO: 3084

Genomic
SEQ ID NO: 4946

Sb09g021540

Sorghum

Polynucleotide
SEQ ID NO: 3085

bicolor

Polypeptide
SEQ ID NO: 3086

Genomic
SEQ ID NO: 4947

Sb09g021920

Sorghum

Polynucleotide
SEQ ID NO: 3087

bicolor

Polypeptide
SEQ ID NO: 3088

Genomic
SEQ ID NO: 4948

Sb09g136020

Sorghum

Polynucleotide
SEQ ID NO: 3089

bicolor

Polypeptide
SEQ ID NO: 3090

Genomic
SEQ ID NO: 4949

Sb09g022360

Sorghum

Polynucleotide
SEQ ID NO: 3091

bicolor

Polypeptide
SEQ ID NO: 3092

Genomic
SEQ ID NO: 4950

Sb09g022370

Sorghum

Polynucleotide
SEQ ID NO: 3093

bicolor

Polypeptide
SEQ ID NO: 3094

Genomic
SEQ ID NO: 4951

Sb09g023580

Sorghum

Polynucleotide
SEQ ID NO: 3095

bicolor

Polypeptide
SEQ ID NO: 3096

Genomic
SEQ ID NO: 4952

Sb09g023650

Sorghum

Polynucleotide
SEQ ID NO: 3097

bicolor

Polypeptide
SEQ ID NO: 3098

Genomic
SEQ ID NO: 4953

Sb09g023840

Sorghum

Polynucleotide
SEQ ID NO: 3099

bicolor

Polypeptide
SEQ ID NO: 3100

Genomic
SEQ ID NO: 4954

Sb09g024390

Sorghum

Polynucleotide
SEQ ID NO: 3101

bicolor

Polypeptide
SEQ ID NO: 3102

Genomic
SEQ ID NO: 4955

Sb09g139040

Sorghum

Polynucleotide
SEQ ID NO: 3103

bicolor

Polypeptide
SEQ ID NO: 3104

Genomic
SEQ ID NO: 4956

Sb09g024810

Sorghum

Polynucleotide
SEQ ID NO: 3105

bicolor

Polypeptide
SEQ ID NO: 3106

Genomic
SEQ ID NO: 4957

Sb09g024990

Sorghum

Polynucleotide
SEQ ID NO: 3107

bicolor

Polypeptide
SEQ ID NO: 3108

Genomic
SEQ ID NO: 4958

Sb09g025150

Sorghum

Polynucleotide
SEQ ID NO: 3109

bicolor

Polypeptide
SEQ ID NO: 3110

Genomic
SEQ ID NO: 4959

Sb09g025190

Sorghum

Polynucleotide
SEQ ID NO: 3111

bicolor

Polypeptide
SEQ ID NO: 3112

Genomic
SEQ ID NO: 4960

Sb09g025250

Sorghum

Polynucleotide
SEQ ID NO: 3113

bicolor

Polypeptide
SEQ ID NO: 3114

Genomic
SEQ ID NO: 4961

Sb09g025400

Sorghum

Polynucleotide
SEQ ID NO: 3115

bicolor

Polypeptide
SEQ ID NO: 3116

Genomic
SEQ ID NO: 4962

Sb09g025430

Sorghum

Polynucleotide
SEQ ID NO: 3117

bicolor

Polypeptide
SEQ ID NO: 3118

Genomic
SEQ ID NO: 4963

Sb09g025520

Sorghum

Polynucleotide
SEQ ID NO: 3119

bicolor

Polypeptide
SEQ ID NO: 3120

Genomic
SEQ ID NO: 4964

Sb09g025790

Sorghum

Polynucleotide
SEQ ID NO: 3121

bicolor

Polypeptide
SEQ ID NO: 3122

Genomic
SEQ ID NO: 4965

Sb09g026020

Sorghum

Polynucleotide
SEQ ID NO: 3123

bicolor

Polypeptide
SEQ ID NO: 3124

Genomic
SEQ ID NO: 4966

Sb09g026120

Sorghum

Polynucleotide
SEQ ID NO: 3125

bicolor

Polypeptide
SEQ ID NO: 3126

Genomic
SEQ ID NO: 4967

Sb09g026780

Sorghum

Polynucleotide
SEQ ID NO: 3127

bicolor

Polypeptide
SEQ ID NO: 3128

Genomic
SEQ ID NO: 4968

Sb09g027010

Sorghum

Polynucleotide
SEQ ID NO: 3129

bicolor

Polypeptide
SEQ ID NO: 3130

Genomic
SEQ ID NO: 4969

Sb09g027030

Sorghum

Polynucleotide
SEQ ID NO: 3131

bicolor

Polypeptide
SEQ ID NO: 3132

Genomic
SEQ ID NO: 4970

Sb09g027040

Sorghum

Polynucleotide
SEQ ID NO: 3133

bicolor

Polypeptide
SEQ ID NO: 3134

Genomic
SEQ ID NO: 4971

Sb09g027060

Sorghum

Polynucleotide
SEQ ID NO: 3135

bicolor

Polypeptide
SEQ ID NO: 3136

Genomic
SEQ ID NO: 4972

Sb09g142860

Sorghum

Polynucleotide
SEQ ID NO: 3137

bicolor

Polypeptide
SEQ ID NO: 3138

Genomic
SEQ ID NO: 4973

Sb09g027380

Sorghum

Polynucleotide
SEQ ID NO: 3139

bicolor

Polypeptide
SEQ ID NO: 3140

Genomic
SEQ ID NO: 4974

Sb09g143020

Sorghum

Polynucleotide
SEQ ID NO: 3141

bicolor

Polypeptide
SEQ ID NO: 3142

Genomic
SEQ ID NO: 4975

Sb09g143660

Sorghum

Polynucleotide
SEQ ID NO: 3143

bicolor

Polypeptide
SEQ ID NO: 3144

Genomic
SEQ ID NO: 4976

Sb09g028120

Sorghum

Polynucleotide
SEQ ID NO: 3145

bicolor

Polypeptide
SEQ ID NO: 3146

Genomic
SEQ ID NO: 4977

Sb09g028130

Sorghum

Polynucleotide
SEQ ID NO: 3147

bicolor

Polypeptide
SEQ ID NO: 3148

Genomic
SEQ ID NO: 4978

Sb09g144220

Sorghum

Polynucleotide
SEQ ID NO: 3149

bicolor

Polypeptide
SEQ ID NO: 3150

Genomic
SEQ ID NO: 4979

Sb09g028400

Sorghum

Polynucleotide
SEQ ID NO: 3151

bicolor

Polypeptide
SEQ ID NO: 3152

Genomic
SEQ ID NO: 4980

Sb09g028540

Sorghum

Polynucleotide
SEQ ID NO: 3153

bicolor

Polypeptide
SEQ ID NO: 3154

Genomic
SEQ ID NO: 4981

Sb09g028650

Sorghum

Polynucleotide
SEQ ID NO: 3155

bicolor

Polypeptide
SEQ ID NO: 3156

Genomic
SEQ ID NO: 4982

Sb09g028780

Sorghum

Polynucleotide
SEQ ID NO: 3157

bicolor

Polypeptide
SEQ ID NO: 3158

Genomic
SEQ ID NO: 4983

Sb09g028840

Sorghum

Polynucleotide
SEQ ID NO: 3159

bicolor

Polypeptide
SEQ ID NO: 3160

Genomic
SEQ ID NO: 4984

Sb09g028940

Sorghum

Polynucleotide
SEQ ID NO: 3161

bicolor

Polypeptide
SEQ ID NO: 3162

Genomic
SEQ ID NO: 4985

Sb09g144920

Sorghum

Polynucleotide
SEQ ID NO: 3163

bicolor

Polypeptide
SEQ ID NO: 3164

Genomic
SEQ ID NO: 4986

Sb09g029030

Sorghum

Polynucleotide
SEQ ID NO: 3165

bicolor

Polypeptide
SEQ ID NO: 3166

Genomic
SEQ ID NO: 4987

Sb09g029400

Sorghum

Polynucleotide
SEQ ID NO: 3167

bicolor

Polypeptide
SEQ ID NO: 3168

Genomic
SEQ ID NO: 4988

Sb09g029840

Sorghum

Polynucleotide
SEQ ID NO: 3169

bicolor

Polypeptide
SEQ ID NO: 3170

Genomic
SEQ ID NO: 4989

Sb09g030140

Sorghum

Polynucleotide
SEQ ID NO: 3171

bicolor

Polypeptide
SEQ ID NO: 3172

Genomic
SEQ ID NO: 4990

Sb09g030570

Sorghum

Polynucleotide
SEQ ID NO: 3173

bicolor

Polypeptide
SEQ ID NO: 3174

Genomic
SEQ ID NO: 4991

Sb09g030720

Sorghum

Polynucleotide
SEQ ID NO: 3175

bicolor

Polypeptide
SEQ ID NO: 3176

Genomic
SEQ ID NO: 4992

Sb09g030750

Sorghum

Polynucleotide
SEQ ID NO: 3177

bicolor

Polypeptide
SEQ ID NO: 3178

Genomic
SEQ ID NO: 4993

Sb09g030830

Sorghum

Polynucleotide
SEQ ID NO: 3179

bicolor

Polypeptide
SEQ ID NO: 3180

Genomic
SEQ ID NO: 4994

Sb09g030840

Sorghum

Polynucleotide
SEQ ID NO: 3181

bicolor

Polypeptide
SEQ ID NO: 3182

Genomic
SEQ ID NO: 4995

Sb1068s002010

Sorghum

Polynucleotide
SEQ ID NO: 3183

bicolor

Polypeptide
SEQ ID NO: 3184

Genomic
SEQ ID NO: 4996

Sb10g000850

Sorghum

Polynucleotide
SEQ ID NO: 3185

bicolor

Polypeptide
SEQ ID NO: 3186

Genomic
SEQ ID NO: 4997

Sb10g000950

Sorghum

Polynucleotide
SEQ ID NO: 3187

bicolor

Polypeptide
SEQ ID NO: 3188

Genomic
SEQ ID NO: 4998

Sb10g001010

Sorghum

Polynucleotide
SEQ ID NO: 3189

bicolor

Polypeptide
SEQ ID NO: 3190

Genomic
SEQ ID NO: 4999

Sb10g003580

Sorghum

Polynucleotide
SEQ ID NO: 3191

bicolor

Polypeptide
SEQ ID NO: 3192

Genomic
SEQ ID NO: 5000

Sb10g001120

Sorghum

Polynucleotide
SEQ ID NO: 3193

bicolor

Polypeptide
SEQ ID NO: 3194

Genomic
SEQ ID NO: 5001

Sb10g001515

Sorghum

Polynucleotide
SEQ ID NO: 3195

bicolor

Polypeptide
SEQ ID NO: 3196

Genomic
SEQ ID NO: 5002

Sb10g001560

Sorghum

Polynucleotide
SEQ ID NO: 3197

bicolor

Polypeptide
SEQ ID NO: 3198

Genomic
SEQ ID NO: 5003

Sb10g001630

Sorghum

Polynucleotide
SEQ ID NO: 3199

bicolor

Polypeptide
SEQ ID NO: 3200

Genomic
SEQ ID NO: 5004

Sb10g001880

Sorghum

Polynucleotide
SEQ ID NO: 3201

bicolor

Polypeptide
SEQ ID NO: 3202

Genomic
SEQ ID NO: 5005

Sb10g002220

Sorghum

Polynucleotide
SEQ ID NO: 3203

bicolor

Polypeptide
SEQ ID NO: 3204

Genomic
SEQ ID NO: 5006

Sb10g002790

Sorghum

Polynucleotide
SEQ ID NO: 3205

bicolor

Polypeptide
SEQ ID NO: 3206

Genomic
SEQ ID NO: 5007

Sb10g006100

Sorghum

Polynucleotide
SEQ ID NO: 3207

bicolor

Polypeptide
SEQ ID NO: 3208

Genomic
SEQ ID NO: 5008

Sb10g006150

Sorghum

Polynucleotide
SEQ ID NO: 3209

bicolor

Polypeptide
SEQ ID NO: 3210

Genomic
SEQ ID NO: 5009

Sb10g003170

Sorghum

Polynucleotide
SEQ ID NO: 3211

bicolor

Polypeptide
SEQ ID NO: 3212

Genomic
SEQ ID NO: 5010

Sb10g003240

Sorghum

Polynucleotide
SEQ ID NO: 3213

bicolor

Polypeptide
SEQ ID NO: 3214

Genomic
SEQ ID NO: 5011

Sb10g003300

Sorghum

Polynucleotide
SEQ ID NO: 3215

bicolor

Polypeptide
SEQ ID NO: 3216

Genomic
SEQ ID NO: 5012

Sb10g003860

Sorghum

Polynucleotide
SEQ ID NO: 3217

bicolor

Polypeptide
SEQ ID NO: 3218

Genomic
SEQ ID NO: 5013

Sb10g004560

Sorghum

Polynucleotide
SEQ ID NO: 3219

bicolor

Polypeptide
SEQ ID NO: 3220

Genomic
SEQ ID NO: 5014

Sb10g004840

Sorghum

Polynucleotide
SEQ ID NO: 3221

bicolor

Polypeptide
SEQ ID NO: 3222

Genomic
SEQ ID NO: 5015

Sb10g004920

Sorghum

Polynucleotide
SEQ ID NO: 3223

bicolor

Polypeptide
SEQ ID NO: 3224

Genomic
SEQ ID NO: 5016

Sb10g006010

Sorghum

Polynucleotide
SEQ ID NO: 3225

bicolor

Polypeptide
SEQ ID NO: 3226

Genomic
SEQ ID NO: 5017

Sb10g006160

Sorghum

Polynucleotide
SEQ ID NO: 3227

bicolor

Polypeptide
SEQ ID NO: 3228

Genomic
SEQ ID NO: 5018

Sb10g006170

Sorghum

Polynucleotide
SEQ ID NO: 3229

bicolor

Polypeptide
SEQ ID NO: 3230

Genomic
SEQ ID NO: 5019

Sb10g006250

Sorghum

Polynucleotide
SEQ ID NO: 3231

bicolor

Polypeptide
SEQ ID NO: 3232

Genomic
SEQ ID NO: 5020

Sb10g006430

Sorghum

Polynucleotide
SEQ ID NO: 3233

bicolor

Polypeptide
SEQ ID NO: 3234

Genomic
SEQ ID NO: 5021

Sb10g006470

Sorghum

Polynucleotide
SEQ ID NO: 3235

bicolor

Polypeptide
SEQ ID NO: 3236

Genomic
SEQ ID NO: 5022

Sb10g013160

Sorghum

Polynucleotide
SEQ ID NO: 3237

bicolor

Polypeptide
SEQ ID NO: 3238

Genomic
SEQ ID NO: 5023

Sb10g006910

Sorghum

Polynucleotide
SEQ ID NO: 3239

bicolor

Polypeptide
SEQ ID NO: 3240

Genomic
SEQ ID NO: 5024

Sb10g007120

Sorghum

Polynucleotide
SEQ ID NO: 3241

bicolor

Polypeptide
SEQ ID NO: 3242

Genomic
SEQ ID NO: 5025

Sb10g007270

Sorghum

Polynucleotide
SEQ ID NO: 3243

bicolor

Polypeptide
SEQ ID NO: 3244

Genomic
SEQ ID NO: 5026

Sb10g007540

Sorghum

Polynucleotide
SEQ ID NO: 3245

bicolor

Polypeptide
SEQ ID NO: 3246

Genomic
SEQ ID NO: 5027

Sb10g007630

Sorghum

Polynucleotide
SEQ ID NO: 3247

bicolor

Polypeptide
SEQ ID NO: 3248

Genomic
SEQ ID NO: 5028

Sb10g007660

Sorghum

Polynucleotide
SEQ ID NO: 3249

bicolor

Polypeptide
SEQ ID NO: 3250

Genomic
SEQ ID NO: 5029

Sb10g008220

Sorghum

Polynucleotide
SEQ ID NO: 3251

bicolor

Polypeptide
SEQ ID NO: 3252

Genomic
SEQ ID NO: 5030

Sb10g008440

Sorghum

Polynucleotide
SEQ ID NO: 3253

bicolor

Polypeptide
SEQ ID NO: 3254

Genomic
SEQ ID NO: 5031

Sb10g008520

Sorghum

Polynucleotide
SEQ ID NO: 3255

bicolor

Polypeptide
SEQ ID NO: 3256

Genomic
SEQ ID NO: 5032

Sb10g008680

Sorghum

Polynucleotide
SEQ ID NO: 3257

bicolor

Polypeptide
SEQ ID NO: 3258

Genomic
SEQ ID NO: 5033

Sb10g008850

Sorghum

Polynucleotide
SEQ ID NO: 3259

bicolor

Polypeptide
SEQ ID NO: 3260

Genomic
SEQ ID NO: 5034

Sb10g008980

Sorghum

Polynucleotide
SEQ ID NO: 3261

bicolor

Polypeptide
SEQ ID NO: 3262

Genomic
SEQ ID NO: 5035

Sb10g009040

Sorghum

Polynucleotide
SEQ ID NO: 3263

bicolor

Polypeptide
SEQ ID NO: 3264

Genomic
SEQ ID NO: 5036

Sb10g009210

Sorghum

Polynucleotide
SEQ ID NO: 3265

bicolor

Polypeptide
SEQ ID NO: 3266

Genomic
SEQ ID NO: 5037

Sb10g009370

Sorghum

Polynucleotide
SEQ ID NO: 3267

bicolor

Polypeptide
SEQ ID NO: 3268

Genomic
SEQ ID NO: 5038

Sb10g010040

Sorghum

Polynucleotide
SEQ ID NO: 3269

bicolor

Polypeptide
SEQ ID NO: 3270

Genomic
SEQ ID NO: 5039

Sb10g010300

Sorghum

Polynucleotide
SEQ ID NO: 3271

bicolor

Polypeptide
SEQ ID NO: 3272

Genomic
SEQ ID NO: 5040

Sb10g010460

Sorghum

Polynucleotide
SEQ ID NO: 3273

bicolor

Polypeptide
SEQ ID NO: 3274

Genomic
SEQ ID NO: 5041

Sb10g010460

Sorghum

Polynucleotide
SEQ ID NO: 3275

bicolor

Polypeptide
SEQ ID NO: 3276

Genomic
SEQ ID NO: 5042

Sb10g010490

Sorghum

Polynucleotide
SEQ ID NO: 3277

bicolor

Polypeptide
SEQ ID NO: 3278

Genomic
SEQ ID NO: 5043

Sb10g010550

Sorghum

Polynucleotide
SEQ ID NO: 3279

bicolor

Polypeptide
SEQ ID NO: 3280

Genomic
SEQ ID NO: 5044

Sb10g010750

Sorghum

Polynucleotide
SEQ ID NO: 3281

bicolor

Polypeptide
SEQ ID NO: 3282

Genomic
SEQ ID NO: 5045

Sb10g011210

Sorghum

Polynucleotide
SEQ ID NO: 3283

bicolor

Polypeptide
SEQ ID NO: 3284

Genomic
SEQ ID NO: 5046

Sb10g011760

Sorghum

Polynucleotide
SEQ ID NO: 3285

bicolor

Polypeptide
SEQ ID NO: 3286

Genomic
SEQ ID NO: 5047

Sb10g012730

Sorghum

Polynucleotide
SEQ ID NO: 3287

bicolor

Polypeptide
SEQ ID NO: 3288

Genomic
SEQ ID NO: 5048

Sb10g012770

Sorghum

Polynucleotide
SEQ ID NO: 3289

bicolor

Polypeptide
SEQ ID NO: 3290

Genomic
SEQ ID NO: 5049

Sb10g050540

Sorghum

Polynucleotide
SEQ ID NO: 3291

bicolor

Polypeptide
SEQ ID NO: 3292

Genomic
SEQ ID NO: 5050

Sb10g013030

Sorghum

Polynucleotide
SEQ ID NO: 3293

bicolor

Polypeptide
SEQ ID NO: 3294

Genomic
SEQ ID NO: 5051

Sb10g013050

Sorghum

Polynucleotide
SEQ ID NO: 3295

bicolor

Polypeptide
SEQ ID NO: 3296

Genomic
SEQ ID NO: 5052

Sb10g016843

Sorghum

Polynucleotide
SEQ ID NO: 3297

bicolor

Polypeptide
SEQ ID NO: 3298

Genomic
SEQ ID NO: 5053

Sb10g019730

Sorghum

Polynucleotide
SEQ ID NO: 3299

bicolor

Polypeptide
SEQ ID NO: 3300

Genomic
SEQ ID NO: 5054

Sb10g019740

Sorghum

Polynucleotide
SEQ ID NO: 3301

bicolor

Polypeptide
SEQ ID NO: 3302

Genomic
SEQ ID NO: 5055

Sb10g020070

Sorghum

Polynucleotide
SEQ ID NO: 3303

bicolor

Polypeptide
SEQ ID NO: 3304

Genomic
SEQ ID NO: 5056

Sb10g020400

Sorghum

Polynucleotide
SEQ ID NO: 3305

bicolor

Polypeptide
SEQ ID NO: 3306

Genomic
SEQ ID NO: 5057

Sb10g020570

Sorghum

Polynucleotide
SEQ ID NO: 3307

bicolor

Polypeptide
SEQ ID NO: 3308

Genomic
SEQ ID NO: 5058

Sb10g115990

Sorghum

Polynucleotide
SEQ ID NO: 3309

bicolor

Polypeptide
SEQ ID NO: 3310

Genomic
SEQ ID NO: 5059

Sb10g021590

Sorghum

Polynucleotide
SEQ ID NO: 3311

bicolor

Polypeptide
SEQ ID NO: 3312

Genomic
SEQ ID NO: 5060

Sb10g122040

Sorghum

Polynucleotide
SEQ ID NO: 3313

bicolor

Polypeptide
SEQ ID NO: 3314

Genomic
SEQ ID NO: 5061

Sb10g021880

Sorghum

Polynucleotide
SEQ ID NO: 3315

bicolor

Polypeptide
SEQ ID NO: 3316

Genomic
SEQ ID NO: 5062

Sb10g021970

Sorghum

Polynucleotide
SEQ ID NO: 3317

bicolor

Polypeptide
SEQ ID NO: 3318

Genomic
SEQ ID NO: 5063

Sb10g022120

Sorghum

Polynucleotide
SEQ ID NO: 3319

bicolor

Polypeptide
SEQ ID NO: 3320

Genomic
SEQ ID NO: 5064

Sb10g022580

Sorghum

Polynucleotide
SEQ ID NO: 3321

bicolor

Polypeptide
SEQ ID NO: 3322

Genomic
SEQ ID NO: 5065

Sb10g023550

Sorghum

Polynucleotide
SEQ ID NO: 3323

bicolor

Polypeptide
SEQ ID NO: 3324

Genomic
SEQ ID NO: 5066

Sb10g023620

Sorghum

Polynucleotide
SEQ ID NO: 3325

bicolor

Polypeptide
SEQ ID NO: 3326

Genomic
SEQ ID NO: 5067

Sb10g023650

Sorghum

Polynucleotide
SEQ ID NO: 3327

bicolor

Polypeptide
SEQ ID NO: 3328

Genomic
SEQ ID NO: 5068

Sb10g023670

Sorghum

Polynucleotide
SEQ ID NO: 3329

bicolor

Polypeptide
SEQ ID NO: 3330

Genomic
SEQ ID NO: 5069

Sb10g023810

Sorghum

Polynucleotide
SEQ ID NO: 3331

bicolor

Polypeptide
SEQ ID NO: 3332

Genomic
SEQ ID NO: 5070

Sb10g024000

Sorghum

Polynucleotide
SEQ ID NO: 3333

bicolor

Polypeptide
SEQ ID NO: 3334

Genomic
SEQ ID NO: 5071

Sb10g024120

Sorghum

Polynucleotide
SEQ ID NO: 3335

bicolor

Polypeptide
SEQ ID NO: 3336

Genomic
SEQ ID NO: 5072

Sb10g131170

Sorghum

Polynucleotide
SEQ ID NO: 3337

bicolor

Polypeptide
SEQ ID NO: 3338

Genomic
SEQ ID NO: 5073

Sb10g024580

Sorghum

Polynucleotide
SEQ ID NO: 3339

bicolor

Polypeptide
SEQ ID NO: 3340

Genomic
SEQ ID NO: 5074

Sb10g024860

Sorghum

Polynucleotide
SEQ ID NO: 3341

bicolor

Polypeptide
SEQ ID NO: 3342

Genomic
SEQ ID NO: 5075

Sb10g025070

Sorghum

Polynucleotide
SEQ ID NO: 3343

bicolor

Polypeptide
SEQ ID NO: 3344

Genomic
SEQ ID NO: 5076

Sb10g133250

Sorghum

Polynucleotide
SEQ ID NO: 3345

bicolor

Polypeptide
SEQ ID NO: 3346

Genomic
SEQ ID NO: 5077

Sb10g025240

Sorghum

Polynucleotide
SEQ ID NO: 3347

bicolor

Polypeptide
SEQ ID NO: 3348

Genomic
SEQ ID NO: 5078

Sb10g025935

Sorghum

Polynucleotide
SEQ ID NO: 3349

bicolor

Polypeptide
SEQ ID NO: 3350

Genomic
SEQ ID NO: 5079

Sb10g026020

Sorghum

Polynucleotide
SEQ ID NO: 3351

bicolor

Polypeptide
SEQ ID NO: 3352

Genomic
SEQ ID NO: 5080

Sb10g026380

Sorghum

Polynucleotide
SEQ ID NO: 3353

bicolor

Polypeptide
SEQ ID NO: 3354

Genomic
SEQ ID NO: 5081

Sb10g026420

Sorghum

Polynucleotide
SEQ ID NO: 3355

bicolor

Polypeptide
SEQ ID NO: 3356

Genomic
SEQ ID NO: 5082

Sb10g026800

Sorghum

Polynucleotide
SEQ ID NO: 3357

bicolor

Polypeptide
SEQ ID NO: 3358

Genomic
SEQ ID NO: 5083

Sb10g137110

Sorghum

Polynucleotide
SEQ ID NO: 3359

bicolor

Polypeptide
SEQ ID NO: 3360

Genomic
SEQ ID NO: 5084

Sb10g027360

Sorghum

Polynucleotide
SEQ ID NO: 3361

bicolor

Polypeptide
SEQ ID NO: 3362

Genomic
SEQ ID NO: 5085

Sb10g027380

Sorghum

Polynucleotide
SEQ ID NO: 3363

bicolor

Polypeptide
SEQ ID NO: 3364

Genomic
SEQ ID NO: 5086

Sb10g027370

Sorghum

Polynucleotide
SEQ ID NO: 3365

bicolor

Polypeptide
SEQ ID NO: 3366

Genomic
SEQ ID NO: 5087

Sb10g027610

Sorghum

Polynucleotide
SEQ ID NO: 3367

bicolor

Polypeptide
SEQ ID NO: 3368

Genomic
SEQ ID NO: 5088

Sb10g027870

Sorghum

Polynucleotide
SEQ ID NO: 3369

bicolor

Polypeptide
SEQ ID NO: 3370

Genomic
SEQ ID NO: 5089

Sb10g028060

Sorghum

Polynucleotide
SEQ ID NO: 3371

bicolor

Polypeptide
SEQ ID NO: 3372

Genomic
SEQ ID NO: 5090

Sb10g028380

Sorghum

Polynucleotide
SEQ ID NO: 3373

bicolor

Polypeptide
SEQ ID NO: 3374

Genomic
SEQ ID NO: 5091

Sb10g028450

Sorghum

Polynucleotide
SEQ ID NO: 3375

bicolor

Polypeptide
SEQ ID NO: 3376

Genomic
SEQ ID NO: 5092

Sb10g028720

Sorghum

Polynucleotide
SEQ ID NO: 3377

bicolor

Polypeptide
SEQ ID NO: 3378

Genomic
SEQ ID NO: 5093

Sb10g029060

Sorghum

Polynucleotide
SEQ ID NO: 3379

bicolor

Polypeptide
SEQ ID NO: 3380

Genomic
SEQ ID NO: 5094

Sb10g029175

Sorghum

Polynucleotide
SEQ ID NO: 3381

bicolor

Polypeptide
SEQ ID NO: 3382

Genomic
SEQ ID NO: 5095

Sb10g029190

Sorghum

Polynucleotide
SEQ ID NO: 3383

bicolor

Polypeptide
SEQ ID NO: 3384

Genomic
SEQ ID NO: 5096

Sb10g029640

Sorghum

Polynucleotide
SEQ ID NO: 3385

bicolor

Polypeptide
SEQ ID NO: 3386

Genomic
SEQ ID NO: 5097

Sb10g029650

Sorghum

Polynucleotide
SEQ ID NO: 3387

bicolor

Polypeptide
SEQ ID NO: 3388

Genomic
SEQ ID NO: 5098

Sb10g029720

Sorghum

Polynucleotide
SEQ ID NO: 3389

bicolor

Polypeptide
SEQ ID NO: 3390

Genomic
SEQ ID NO: 5099

Sb10g030240

Sorghum

Polynucleotide
SEQ ID NO: 3391

bicolor

Polypeptide
SEQ ID NO: 3392

Genomic
SEQ ID NO: 5100

Sb10g031070

Sorghum

Polynucleotide
SEQ ID NO: 3393

bicolor

Polypeptide
SEQ ID NO: 3394

Genomic
SEQ ID NO: 5101

Sb10g031300

Sorghum

Polynucleotide
SEQ ID NO: 3395

bicolor

Polypeptide
SEQ ID NO: 3396

Genomic
SEQ ID NO: 5102

Sb1676s002010

Sorghum

Polynucleotide
SEQ ID NO: 3397

bicolor

Polypeptide
SEQ ID NO: 3398

Genomic
SEQ ID NO: 5103

Sb2674s002010

Sorghum

Polynucleotide
SEQ ID NO: 3399

bicolor

Polypeptide
SEQ ID NO: 3400

Genomic
SEQ ID NO: 5104

dpzm00g103644

Zea mays

Polynucleotide
SEQ ID NO: 3401

Polypeptide
SEQ ID NO: 3402

sbiMIR156B

Sorghum

Polynucleotide
SEQ ID NO: 3403

bicolor

Genomic
SEQ ID NO: 5105

ADH1YNT1PA

Pichia angusta

Polynucleotide
SEQ ID NO: 3404

Construction of Nucleic Acids

The isolated nucleic acids of the present disclosure can be made using (a) standard recombinant methods, (b) synthetic techniques or combinations thereof. In some embodiments, the polynucleotides of the present disclosure will be cloned, amplified or otherwise constructed from a fungus or bacteria.

The nucleic acids may conveniently comprise sequences in addition to a polynucleotide of the present disclosure. For example, a multi-cloning site comprising one or more endonuclease restriction sites may be inserted into the nucleic acid to aid in isolation of the polynucleotide. Also, translatable sequences may be inserted to aid in the isolation of the translated polynucleotide of the present disclosure. For example, a hexa-histidine marker sequence provides a convenient means to purify the proteins of the present disclosure. The nucleic acid of the present disclosure—excluding the polynucleotide sequence—is optionally a vector, adapter or linker for cloning and/or expression of a polynucleotide of the present disclosure. Additional sequences may be added to such cloning and/or expression sequences to optimize their function in cloning and/or expression, to aid in isolation of the polynucleotide or to improve the introduction of the polynucleotide into a cell. Typically, the length of a nucleic acid of the present disclosure less the length of its polynucleotide of the present disclosure is less than 20 kilobase pairs, often less than 15 kb, and frequently less than 10 kb. Use of cloning vectors, expression vectors, adapters, and linkers is well known in the art. Exemplary nucleic acids include such vectors as: M13, lambda ZAP Express, lambda ZAP II, lambda gt10, lambda gt11, pBK-CMV, pBK-RSV, pBluescript II, lambda DASH II, lambda EMBL 3, lambda EMBL 4, pWE15, SuperCos 1, SurfZap, Uni-ZAP, pBC, pBS+/−, pSG5, pBK, pCR-Script, pET, pSPUTK, p3′SS, pGEM, pSK+/−, pGEX, pSPORTI and II, pOPRSVI CAT, pOPI3 CAT, pXT1, pSG5, pPbac, pMbac, pMC1neo, pOG44, pOG45, pFRTβGAL, pNEOβGAL, pRS403, pRS404, pRS405, pRS406, pRS413, pRS414, pRS415, pRS416, lambda MOSSIox and lambda MOSEIox. Optional vectors for the present disclosure, include but are not limited to, lambda ZAP II and pGEX. For a description of various nucleic acids see, e.g., Stratagene Cloning Systems, Catalogs 1995, 1996, 1997 (La Jolla, Calif.); and, Amersham Life Sciences, Inc, Catalog '97 (Arlington Heights, Ill.).

Synthetic Methods for Constructing Nucleic Acids

The isolated nucleic acids of the present disclosure can also be prepared by direct chemical synthesis by methods such as the phosphotriester method of Narang, et al., (1979) Meth. Enzymol. 68:90-9; the phosphodiester method of Brown, et al., (1979) Meth. Enzymol. 68:109-51; the diethylphosphoramidite method of Beaucage, et al., (1981) Tetra. Letts. 22(20):1859-62; the solid phase phosphoramidite triester method described by Beaucage, et al., supra, e.g., using an automated synthesizer, e.g., as described in Needham-VanDevanter, et al., (1984) Nucleic Acids Res. 12:6159-68 and the solid support method of U.S. Pat. No. 4,458,066. Chemical synthesis generally produces a single stranded oligonucleotide. This may be converted into double stranded DNA by hybridization with a complementary sequence or by polymerization with a DNA polymerase using the single strand as a template. One of skill will recognize that while chemical synthesis of DNA is limited to sequences of about 100 bases, longer sequences may be obtained by the ligation of shorter sequences.

UTRs and Codon Preference

In general, translational efficiency has been found to be regulated by specific sequence elements in the 5′ non-coding or untranslated region (5′ UTR) of the RNA. Positive sequence motifs include translational initiation consensus sequences (Kozak, (1987) Nucleic Acids Res. 15:8125) and the 5<G> 7 methyl GpppG RNA cap structure (Drummond, et al., (1985) Nucleic Acids Res. 13:7375). Negative elements include stable intramolecular 5′ UTR stem-loop structures (Muesing, et al., (1987) Cell 48:691) and AUG sequences or short open reading frames preceded by an appropriate AUG in the 5′ UTR (Kozak, supra, Rao, et al., (1988) Mol. and Cell. Biol. 8:284). Accordingly, the present disclosure provides 5′ and/or 3′ UTR regions for modulation of translation of heterologous coding sequences.

Further, the polypeptide-encoding segments of the polynucleotides of the present disclosure can be modified to alter codon usage. Altered codon usage can be employed to alter translational efficiency and/or to optimize the coding sequence for expression in a desired host or to optimize the codon usage in a heterologous sequence for expression in maize. Codon usage in the coding regions of the polynucleotides of the present disclosure can be analyzed statistically using commercially available software packages such as “Codon Preference” available from the University of Wisconsin Genetics Computer Group. See, Devereaux, et al., (1984) Nucleic Acids Res. 12:387-395; or MacVector 4.1 (Eastman Kodak Co., New Haven, Conn.). Thus, the present disclosure provides a codon usage frequency characteristic of the coding region of at least one of the polynucleotides of the present disclosure. The number of polynucleotides (3 nucleotides per amino acid) that can be used to determine a codon usage frequency can be any integer from 3 to the number of polynucleotides of the present disclosure as provided herein. Optionally, the polynucleotides will be full-length sequences. An exemplary number of sequences for statistical analysis can be at least 1, 5, 10, 20, 50 or 100.

Sequence Shuffling

The present disclosure provides methods for sequence shuffling using polynucleotides of the present disclosure, and compositions resulting therefrom. Sequence shuffling is described in PCT Publication Number 1996/19256. See also, Zhang, et al., (1997) Proc. Natl. Acad. Sci. USA 94:4504-9 and Zhao, et al., (1998) Nature Biotech 16:258-61. Generally, sequence shuffling provides a means for generating libraries of polynucleotides having a desired characteristic, which can be selected or screened for. Libraries of recombinant polynucleotides are generated from a population of related sequence polynucleotides, which comprise sequence regions, which have substantial sequence identity and can be homologously recombined in vitro or in vivo. The population of sequence-recombined polynucleotides comprises a subpopulation of polynucleotides which possess desired or advantageous characteristics and which can be selected by a suitable selection or screening method. The characteristics can be any property or attribute capable of being selected for or detected in a screening system and may include properties of: an encoded protein, a transcriptional element, a sequence controlling transcription, RNA processing, RNA stability, chromatin conformation, translation or other expression property of a gene or transgene, a replicative element, a protein-binding element, or the like, such as any feature which confers a selectable or detectable property. In some embodiments, the selected characteristic will be an altered K_mand/or K_catover the wild-type protein as provided herein. In other embodiments, a protein or polynucleotide generated from sequence shuffling will have a ligand binding affinity greater than the non-shuffled wild-type polynucleotide. In yet other embodiments, a protein or polynucleotide generated from sequence shuffling will have an altered pH optimum as compared to the non-shuffled wild-type polynucleotide. The increase in such properties can be at least 110%, 120%, 130%, 140% or greater than 150% of the wild-type value.

Recombinant Expression Cassettes

The present disclosure further provides recombinant expression cassettes comprising a nucleic acid of the present disclosure. A nucleic acid sequence coding for the desired polynucleotide of the present disclosure, for example a cDNA or a genomic sequence encoding a polypeptide long enough to code for an active protein of the present disclosure, can be used to construct a recombinant expression cassette which can be introduced into the desired host cell. A recombinant expression cassette will typically comprise a polynucleotide of the present disclosure operably linked to transcriptional initiation regulatory sequences which will direct the transcription of the polynucleotide in the intended host cell, such as tissues of a transformed plant.

For example, plant expression vectors may include (1) a cloned plant gene under the transcriptional control of 5′ and 3′ regulatory sequences and (2) a dominant selectable marker. Such plant expression vectors may also contain, if desired, a promoter regulatory region (e.g., one conferring inducible or constitutive, environmentally- or developmentally-regulated, or cell- or tissue-specific/selective expression), a transcription initiation start site, a ribosome binding site, an RNA processing signal, a transcription termination site and/or a polyadenylation signal.

A plant promoter fragment can be employed which will direct expression of a polynucleotide of the present disclosure in all tissues of a regenerated plant. Such promoters are referred to herein as “constitutive” promoters and are active under most environmental conditions and states of development or cell differentiation. Examples of constitutive promoters include the 1′- or 2′-promoter derived from T-DNA of Agrobacterium tumefaciens, the Smas promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No. 5,683,439), the Nos promoter, the rubisco promoter, the GRP1-8 promoter, the 35S promoter from cauliflower mosaic virus (CaMV), as described in Odell, et al., (1985) Nature 313:810-2; rice actin (McElroy, et al., (1990) Plant Cell 163-171); ubiquitin (Christensen, et al., (1992) Plant Mol. Biol. 12:619-632 and Christensen, et al., (1992) Plant Mol. Biol. 18:675-89); pEMU (Last, et al., (1991) Theor. Appl. Genet. 81:581-8); MAS (Velten, et al., (1984) EMBO J. 3:2723-30) and maize H3 histone (Lepetit, et al., (1992) Mol. Gen. Genet. 231:276-85 and Atanassvoa, et al., (1992) Plant Journal 2(3):291-300); ALS promoter, as described in PCT Application Number WO 1996/30530; GOS2 (U.S. Pat. No. 6,504,083) and other transcription initiation regions from various plant genes known to those of skill. For the present disclosure ubiquitin is the preferred promoter for expression in monocot plants.

Alternatively, the plant promoter can direct expression of a polynucleotide of the present disclosure in a specific tissue or may be otherwise under more precise environmental or developmental control. Such promoters are referred to here as “inducible” promoters (Rab17, RAD29). Environmental conditions that may affect transcription by inducible promoters include pathogen attack, anaerobic conditions or the presence of light. Examples of inducible promoters are the Adh1 promoter, which is inducible by hypoxia or cold stress, the Hsp70 promoter, which is inducible by heat stress and the PPDK promoter, which is inducible by light.

Examples of promoters under developmental control include promoters that initiate transcription only, or preferentially, in certain tissues, such as leaves, roots, fruit, seeds or flowers. The operation of a promoter may also vary depending on its location in the genome. Thus, an inducible promoter may become fully or partially constitutive in certain locations.

If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3′-end of a polynucleotide coding region. The polyadenylation region can be derived from a variety of plant genes or from T-DNA. The 3′ end sequence to be added can be derived from, for example, the nopaline synthase or octopine synthase genes or alternatively from another plant gene or less preferably from any other eukaryotic gene. Examples of such regulatory elements include, but are not limited to, 3′ termination and/or polyadenylation regions such as those of the Agrobacterium tumefaciens nopaline synthase (nos) gene (Bevan, et al., (1983) Nucleic Acids Res. 12:369-85); the potato proteinase inhibitor II (PINII) gene (Keil, et al., (1986) Nucleic Acids Res. 14:5641-50 and An, et al., (1989) Plant Cell 1:115-22) and the CaMV 19S gene (Mogen, et al., (1990) Plant Cell 2:1261-72).

An intron sequence can be added to the 5′ untranslated region or the coding sequence of the partial coding sequence to increase the amount of the mature message that accumulates in the cytosol. Inclusion of a spliceable intron in the transcription unit in both plant and animal expression constructs has been shown to increase gene expression at both the mRNA and protein levels up to 1000-fold (Buchman and Berg, (1988) Mol. Cell Biol. 8:4395-4405; Callis, et al., (1987) Genes Dev. 1:1183-200). Such intron enhancement of gene expression is typically greatest when placed near the 5′ end of the transcription unit. Use of maize introns Adh1-S intron 1, 2 and 6, the Bronze-1 intron are known in the art. See generally, THE MAIZE HANDBOOK, Chapter 116, Freeling and Walbot, eds., Springer, New York (1994).

Plant signal sequences, including, but not limited to, signal-peptide encoding DNA/RNA sequences which target proteins to the extracellular matrix of the plant cell (Dratewka-Kos, et al., (1989) J. Biol. Chem. 264:4896-900), such as the Nicotiana plumbaginifolia extension gene (DeLoose, et al., (1991) Gene 99:95-100); signal peptides which target proteins to the vacuole, such as the sweet potato sporamin gene (Matsuka, et al., (1991) Proc. Natl. Acad. Sci. USA 88:834) and the barley lectin gene (Wilkins, et al., (1990) Plant Cell, 2:301-13); signal peptides which cause proteins to be secreted, such as that of PRIb (Lind, et al., (1992) Plant Mol. Biol. 18:47-53) or the barley alpha amylase (BAA) (Rahmatullah, et al., (1989) Plant Mol. Biol. 12:119, and hereby incorporated by reference) or signal peptides which target proteins to the plastids such as that of rapeseed enoyl-Acp reductase (Verwaert, et al., (1994) Plant Mol. Biol. 26:189-202) are useful in the disclosure.

The vector comprising the sequences from a polynucleotide of the present disclosure will typically comprise a marker gene, which confers a selectable phenotype on plant cells. Usually, the selectable marker gene will encode antibiotic resistance, with suitable genes including genes coding for resistance to the antibiotic spectinomycin (e.g., the aada gene), the streptomycin phosphotransferase (SPT) gene coding for streptomycin resistance, the neomycin phosphotransferase (NPTII) gene encoding kanamycin or geneticin resistance, the hygromycin phosphotransferase (HPT) gene coding for hygromycin resistance, genes coding for resistance to herbicides which act to inhibit the action of acetolactate synthase (ALS), in particular the sulfonylurea-type herbicides (e.g., the acetolactate synthase (ALS) gene containing mutations leading to such resistance in particular the S4 and/or Hra mutations), genes coding for resistance to herbicides which act to inhibit action of glutamine synthase, such as phosphinothricin or basta (e.g., the bar gene) or other such genes known in the art. The bar gene encodes resistance to the herbicide basta and the ALS gene encodes resistance to the herbicide chlorsulfuron.

Typical vectors useful for expression of genes in higher plants are well known in the art and include vectors derived from the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens described by Rogers, et al., (1987) Meth. Enzymol. 153:253-77. These vectors are plant integrating vectors in that on transformation, the vectors integrate a portion of vector DNA into the genome of the host plant. Exemplary A. tumefaciens vectors useful herein are plasmids pKYLX6 and pKYLX7 of Schardl, et al., (1987) Gene 61:1-11 and Berger, et al., (1989) Proc. Natl. Acad. Sci. USA, 86:8402-6. Another useful vector herein is plasmid pBI101.2 that is available from CLONTECH Laboratories, Inc. (Palo Alto, Calif.).

Expression of Proteins in Host Cells

Using the nucleic acids of the present disclosure, one may express a protein of the present disclosure in a recombinantly engineered cell such as bacteria, yeast, insect, mammalian or preferably plant cells. The cells produce the protein in a non-natural condition (e.g., in quantity, composition, location and/or time), because they have been genetically altered through human intervention to do so.

It is expected that those of skill in the art are knowledgeable in the numerous expression systems available for expression of a nucleic acid encoding a protein of the present disclosure. No attempt to describe in detail the various methods known for the expression of proteins in prokaryotes or eukaryotes will be made.

In brief summary, the expression of isolated nucleic acids encoding a protein of the present disclosure will typically be achieved by operably linking, for example, the DNA or cDNA to a promoter (which is either constitutive or inducible), followed by incorporation into an expression vector. The vectors can be suitable for replication and integration in either prokaryotes or eukaryotes. Typical expression vectors contain transcription and translation terminators, initiation sequences, and promoters useful for regulation of the expression of the DNA encoding a protein of the present disclosure. To obtain high level expression of a cloned gene, it is desirable to construct expression vectors which contain, at the minimum, a strong promoter, such as ubiquitin, to direct transcription, a ribosome binding site for translational initiation and a transcription/translation terminator. Constitutive promoters are classified as providing for a range of constitutive expression. Thus, some are weak constitutive promoters and others are strong constitutive promoters. Generally, by “weak promoter” is intended a promoter that drives expression of a coding sequence at a low level. By “low level” is intended at levels of about 1/10,000 transcripts to about 1/100,000 transcripts to about 1/500,000 transcripts. Conversely, a “strong promoter” drives expression of a coding sequence at a “high level” or about 1/10 transcripts to about 1/100 transcripts to about 1/1,000 transcripts.

One of skill would recognize that modifications could be made to a protein of the present disclosure without diminishing its biological activity. Some modifications may be made to facilitate the cloning, expression or incorporation of the targeting molecule into a fusion protein. Such modifications are well known to those of skill in the art and include, for example, a methionine added at the amino terminus to provide an initiation site or additional amino acids (e.g., poly His) placed on either terminus to create conveniently located restriction sites or termination codons or purification sequences.

Expression in Prokaryotes

Prokaryotic cells may be used as hosts for expression. Prokaryotes most frequently are represented by various strains of E. coli; however, other microbial strains may also be used. Commonly used prokaryotic control sequences which are defined herein to include promoters for transcription initiation, optionally with an operator, along with ribosome binding site sequences, include such commonly used promoters as the beta lactamase (penicillinase) and lactose (lac) promoter systems (Chang, et al., (1977) Nature 198:1056), the tryptophan (trp) promoter system (Goeddel, et al., (1980) Nucleic Acids Res. 8:4057) and the lambda derived P L promoter and N-gene ribosome binding site (Shimatake, et al., (1981) Nature 292:128). The inclusion of selection markers in DNA vectors transfected in E. coli is also useful. Examples of such markers include genes specifying resistance to ampicillin, tetracycline or chloramphenicol.

The vector is selected to allow introduction of the gene of interest into the appropriate host cell. Bacterial vectors are typically of plasmid or phage origin. Appropriate bacterial cells are infected with phage vector particles or transfected with naked phage vector DNA. If a plasmid vector is used, the bacterial cells are transfected with the plasmid vector DNA. Expression systems for expressing a protein of the present disclosure are available using Bacillus sp. and Salmonella (Palva, et al., (1983) Gene 22:229-35; Mosbach, et al., (1983) Nature 302:543-5). The pGEX-4T-1 plasmid vector from Pharmacia is the preferred E. coli expression vector for the present disclosure.

Expression in Eukaryotes

A variety of eukaryotic expression systems such as yeast, insect cell lines, plant and mammalian cells, are known to those of skill in the art. As explained briefly below, the present disclosure can be expressed in these eukaryotic systems. In some embodiments, transformed/transfected plant cells, as discussed infra, are employed as expression systems for production of the proteins of the instant disclosure.

Synthesis of heterologous proteins in yeast is well known. Sherman, et al., (1982) METHODS IN YEAST GENETICS, Cold Spring Harbor Laboratory is a well recognized work describing the various methods available to produce the protein in yeast. Two widely utilized yeasts for production of eukaryotic proteins are Saccharomyces cerevisiae and Pichia pastoris. Vectors, strains and protocols for expression in Saccharomyces and Pichia are known in the art and available from commercial suppliers (e.g., Invitrogen). Suitable vectors usually have expression control sequences, such as promoters, including 3-phosphoglycerate kinase or alcohol oxidase and an origin of replication, termination sequences and the like as desired.

A protein of the present disclosure, once expressed, can be isolated from yeast by lysing the cells and applying standard protein isolation techniques to the lysates or the pellets. The monitoring of the purification process can be accomplished by using Western blot techniques or radioimmunoassay of other standard immunoassay techniques.

The sequences encoding proteins of the present disclosure can also be ligated to various expression vectors for use in transfecting cell cultures of, for instance, mammalian, insect or plant origin. Mammalian cell systems often will be in the form of monolayers of cells although mammalian cell suspensions may also be used. A number of suitable host cell lines capable of expressing intact proteins have been developed in the art, and include the HEK293, BHK21 and CHO cell lines. Expression vectors for these cells can include expression control sequences, such as an origin of replication, a promoter (e.g., the CMV promoter, a HSV tk promoter or pgk (phosphoglycerate kinase) promoter), an enhancer (Queen, et al., (1986) Immunol. Rev. 89:49) and necessary processing information sites, such as ribosome binding sites, RNA splice sites, polyadenylation sites (e.g., an SV40 large T Ag poly A addition site) and transcriptional terminator sequences. Other animal cells useful for production of proteins of the present disclosure are available, for instance, from the American Type Culture Collection Catalogue of Cell Lines and Hybridomas (7^thed., 1992).

Appropriate vectors for expressing proteins of the present disclosure in insect cells are usually derived from the SF9 baculovirus. Suitable insect cell lines include mosquito larvae, silkworm, armyworm, moth, and Drosophila cell lines such as a Schneider cell line (see, e.g., Schneider, (1987) J. Embryol. Exp. Morphol. 27:353-65).

As with yeast, when higher animal or plant host cells are employed, polyadenlyation or transcription terminator sequences are typically incorporated into the vector. An example of a terminator sequence is the polyadenlyation sequence from the bovine growth hormone gene. Sequences for accurate splicing of the transcript may also be included. An example of a splicing sequence is the VP1 intron from SV40 (Sprague, et al., (1983) J. Virol. 45:773-81). Additionally, gene sequences to control replication in the host cell may be incorporated into the vector such as those found in bovine papilloma virus type-vectors (Saveria-Campo, “Bovine Papilloma Virus DNA a Eukaryotic Cloning Vector,” in DNA CLONING: A PRACTICAL APPROACH, vol. II, Glover, ed., IRL Press, Arlington, Va., pp. 213-38 (1985)).

In addition, the gene for yield improvement placed in the appropriate plant expression vector can be used to transform plant cells. The polypeptide can then be isolated from plant callus or the transformed cells can be used to regenerate transgenic plants. Such transgenic plants can be harvested, and the appropriate tissues (seed or leaves, for example) can be subjected to large scale protein extraction and purification techniques.

Plant Transformation Methods

Numerous methods for introducing foreign genes into plants are known and can be used to insert a yield improvement polynucleotide into a plant host, including biological and physical plant transformation protocols. See, e.g., Miki, et al., “Procedure for Introducing Foreign DNA into Plants,” in METHODS IN PLANT MOLECULAR BIOLOGY AND BIOTECHNOLOGY, Glick and Thompson, eds., CRC Press, Inc., Boca Raton, pp. 67-88 (1993). The methods chosen vary with the host plant, and include chemical transfection methods such as calcium phosphate, microorganism-mediated gene transfer such as Agrobacterium (Horsch, et al., (1985) Science 227:1229-31), electroporation, micro-injection and biolistic bombardment.

Expression cassettes and vectors and in vitro culture methods for plant cell or tissue transformation and regeneration of plants are known and available. See, e.g., Gruber, et al., “Vectors for Plant Transformation,” in METHODS IN PLANT MOLECULAR BIOLOGY AND BIOTECHNOLOGY, supra, pp. 89-119.

The isolated polynucleotides or polypeptides may be introduced into the plant by one or more techniques typically used for direct delivery into cells. Such protocols may vary depending on the type of organism, cell, plant or plant cell, i.e., monocot or dicot, targeted for gene modification. Suitable methods of transforming plant cells include microinjection (Crossway, et al., (1986) Biotechniques 4:320-334 and U.S. Pat. No. 6,300,543), electroporation (Riggs, et al., (1986) Proc. Natl. Acad. Sci. USA 83:5602-5606), direct gene transfer (Paszkowski, et al., (1984) EMBO J. 3:2717-2722) and ballistic particle acceleration (see, for example, Sanford, et al., U.S. Pat. No. 4,945,050; WO 1991/10725 and McCabe, et al., (1988) Biotechnology 6:923-926). Also see, Tomes, et al., Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment. pp. 197-213 in Plant Cell, Tissue and Organ Culture, Fundamental Methods eds. Gamborg and Phillips, Springer-Verlag Berlin Heidelberg New York, 1995; U.S. Pat. No. 5,736,369 (meristem); Weissinger, et al., (1988) Ann. Rev. Genet. 22:421-477; Sanford, et al., (1987) Particulate Science and Technology 5:27-37 (onion); Christou, et al., (1988) Plant Physiol. 87:671-674 (soybean); Datta, et al., (1990) Biotechnology 8:736-740 (rice); Klein, et al., (1988) Proc. Natl. Acad. Sci. USA 85:4305-4309 (maize); Klein, et al., (1988) Biotechnology 6:559-563 (maize); WO 1991/10725 (maize); Klein, et al., (1988) Plant Physiol. 91:440-444 (maize); Fromm, et al., (1990) Biotechnology 8:833-839 and Gordon-Kamm, et al., (1990) Plant Cell 2:603-618 (maize); Hooydaas-Van Slogteren and Hooykaas, (1984) Nature (London) 311:763-764; Bytebier, et al., (1987) Proc. Natl. Acad. Sci. USA 84:5345-5349 (Liliaceae); De Wet, et al., (1985) In The Experimental Manipulation of Ovule Tissues, ed. Chapman, et al., pp. 197-209; Longman, N Y (pollen); Kaeppler, et al., (1990) Plant Cell Reports 9:415-418 and Kaeppler, et al., (1992) Theor. Appl. Genet. 84:560-566 (whisker-mediated transformation); U.S. Pat. No. 5,693,512 (sonication); D'Halluin, et al., (1992) Plant Cell 4:1495-1505 (electroporation); Li, et al., (1993) Plant Cell Reports 12:250-255 and Christou and Ford, (1995) Annals of Botany 75:407-413 (rice); Osjoda, et al., (1996) Nature Biotech. 14:745-750; Agrobacterium mediated maize transformation (U.S. Pat. No. 5,981,840); silicon carbide whisker methods (Frame, et al., (1994) Plant J. 6:941-948); laser methods (Guo, et al., (1995) Physiologia Plantarum 93:19-24); sonication methods (Bao, et al., (1997) Ultrasound in Medicine & Biology 23:953-959; Finer and Finer, (2000) Lett Appl Microbiol. 30:406-10; Amoah, et al., (2001) J Exp Bot 52:1135-42); polyethylene glycol methods (Krens, et al., (1982) Nature 296:72-77); protoplasts of monocot and dicot cells can be transformed using electroporation (Fromm, et al., (1985) Proc. Natl. Acad. Sci. USA 82:5824-5828) and microinjection (Crossway, et al., (1986) Mol. Gen. Genet. 202:179-185), all of which are herein incorporated by reference.

Agrobacterium-Mediated Transformation

The most widely utilized method for introducing an expression vector into plants is based on the natural transformation system of Agrobacterium. A. tumefaciens and A. rhizogenes are plant pathogenic soil bacteria, which genetically transform plant cells. The Ti and Ri plasmids of A. tumefaciens and A. rhizogenes, respectively, carry genes responsible for genetic transformation of plants. See, e.g., Kado, (1991) Crit. Rev. Plant Sci. 10:1. Descriptions of the Agrobacterium vector systems and methods for Agrobacterium-mediated gene transfer are provided in Gruber, et al., supra; Miki, et al., supra; and Moloney, et al., (1989) Plant Cell Reports 8:238.

Similarly, the gene can be inserted into the T-DNA region of a Ti or Ri plasmid derived from A. tumefaciens or A. rhizogenes, respectively. Thus, expression cassettes can be constructed as above, using these plasmids. Many control sequences are known which when coupled to a heterologous coding sequence and transformed into a host organism show fidelity in gene expression with respect to tissue/organ specificity of the original coding sequence. See, e.g., Benfey and Chua, (1989) Science 244:174-81. Particularly suitable control sequences for use in these plasmids are promoters for constitutive leaf-specific expression of the gene in the various target plants. Other useful control sequences include a promoter and terminator from the nopaline synthase gene (NOS). The NOS promoter and terminator are present in the plasmid pARC2, available from the American Type Culture Collection and designated ATCC 67238. If such a system is used, the virulence (vir) gene from either the Ti or Ri plasmid must also be present, either along with the T-DNA portion or via a binary system where the vir gene is present on a separate vector. Such systems, vectors for use therein, and methods of transforming plant cells are described in U.S. Pat. No. 4,658,082; U.S. patent application Ser. No. 913,914, filed Oct. 1, 1986, as referenced in U.S. Pat. No. 5,262,306, issued Nov. 16, 1993 and Simpson, et al., (1986) Plant Mol. Biol. 6:403-15 (also referenced in the '306 patent), all incorporated by reference in their entirety.

Once constructed, these plasmids can be placed into A. rhizogenes or A. tumefaciens and these vectors used to transform cells of plant species, which are ordinarily susceptible to Fusarium or Alternaria infection. Several other transgenic plants are also contemplated by the present disclosure including but not limited to soybean, corn, sorghum, alfalfa, rice, clover, cabbage, banana, coffee, celery, tobacco, cowpea, cotton, melon and pepper. The selection of either A. tumefaciens or A. rhizogenes will depend on the plant being transformed thereby. In general A. tumefaciens is the preferred organism for transformation. Most dicotyledonous plants, some gymnosperms, and a few monocotyledonous plants (e.g., certain members of the Liliales and Arales) are susceptible to infection with A. tumefaciens. A. rhizogenes also has a wide host range, embracing most dicots and some gymnosperms, which includes members of the Leguminosae, Compositae and Chenopodiaceae. Monocot plants can now be transformed with some success. EP Patent Application Number 604 662 A1 discloses a method for transforming monocots using Agrobacterium. EP Patent Application Number 672 752 A1 discloses a method for transforming monocots with Agrobacterium using the scutellum of immature embryos. Ishida, et al., discuss a method for transforming maize by exposing immature embryos to A. tumefaciens (Nature Biotechnology 14:745-50 (1996)).

Once transformed, these cells can be used to regenerate transgenic plants. For example, whole plants can be infected with these vectors by wounding the plant and then introducing the vector into the wound site. Any part of the plant can be wounded, including leaves, stems and roots. Alternatively, plant tissue, in the form of an explant, such as cotyledonary tissue or leaf disks, can be inoculated with these vectors and cultured under conditions, which promote plant regeneration. Roots or shoots transformed by inoculation of plant tissue with A. rhizogenes or A. tumefaciens, containing the gene coding for the fumonisin degradation enzyme, can be used as a source of plant tissue to regenerate fumonisin-resistant transgenic plants, either via somatic embryogenesis or organogenesis. Examples of such methods for regenerating plant tissue are disclosed in Shahin, Theor. Appl. Genet. 69:235-40 (1985); U.S. Pat. No. 4,658,082; Simpson, et al., supra and U.S. patent application Ser. Nos. 913,913 and 913,914, both filed Oct. 1, 1986, as referenced in U.S. Pat. No. 5,262,306, issued Nov. 16, 1993, the entire disclosures therein incorporated herein by reference.

Direct Gene Transfer

Despite the fact that the host range for Agrobacterium-mediated transformation is broad, some major cereal crop species and gymnosperms have generally been recalcitrant to this mode of gene transfer, even though some success has recently been achieved in rice (Hiei, et al., (1994) The Plant Journal 6:271-82). Several methods of plant transformation, collectively referred to as direct gene transfer, have been developed as an alternative to Agrobacterium-mediated transformation.

A generally applicable method of plant transformation is microprojectile-mediated transformation, where DNA is carried on the surface of microprojectiles measuring about 1 to 4 μm. The expression vector is introduced into plant tissues with a biolistic device that accelerates the microprojectiles to speeds of 300 to 600 m/s which is sufficient to penetrate the plant cell walls and membranes (Sanford, et al., (1987) Part. Sci. Technol. 5:27; Sanford, (1988) Trends Biotech 6:299; Sanford, (1990) Physiol. Plant 79:206 and Klein, et al., (1992) Biotechnology 10:268).

Another method for physical delivery of DNA to plants is sonication of target cells as described in Zang, et al., (1991) BioTechnology 9:996. Alternatively, liposome or spheroplast fusions have been used to introduce expression vectors into plants. See, e.g., Deshayes, et al., (1985) EMBO J. 4:2731 and Christou, et al., (1987) Proc. Natl. Acad. Sci. USA 84:3962. Direct uptake of DNA into protoplasts using CaCl₂precipitation, polyvinyl alcohol or poly-L-ornithine has also been reported. See, e.g., Hain, et al., (1985) Mol. Gen. Genet. 199:161 and Draper, et al., (1982) Plant Cell Physiol. 23:451.

Electroporation of protoplasts and whole cells and tissues has also been described. See, e.g., Donn, et al., (1990) in Abstracts of the VIIth Intl. Congress on Plant Cell and Tissue Culture IAPTC, A2-38, p. 53; D'Halluin, et al., (1992) Plant Cell 4:1495-505 and Spencer, et al., (1994) Plant Mol. Biol. 24:51-61.

Increasing the Activity and/or Level of a Yield Improvement Polypeptide

Methods are provided to increase the activity and/or level of the yield improvement polypeptide of the disclosure. An increase in the level and/or activity of the yield improvement polypeptide of the disclosure can be achieved by providing to the plant a yield improvement polypeptide. The yield improvement polypeptide can be provided by introducing the amino acid sequence encoding the yield improvement polypeptide into the plant, introducing into the plant a nucleotide sequence encoding an yield improvement polypeptide or alternatively by modifying a genomic locus encoding the yield improvement polypeptide of the disclosure.

As discussed elsewhere herein, many methods are known the art for providing a polypeptide to a plant including, but not limited to, direct introduction of the polypeptide into the plant, introducing into the plant (transiently or stably) a polynucleotide construct encoding a polypeptide having cell number regulator activity. It is also recognized that the methods of the disclosure may employ a polynucleotide that is not capable of directing, in the transformed plant, the expression of a protein or an RNA. Thus, the level and/or activity of an yield improvement polypeptide may be increased by altering the gene encoding the yield improvement polypeptide or its promoter. See, e.g., Kmiec, U.S. Pat. No. 5,565,350; Zarling, et al., PCT/US93/03868. Therefore mutagenized plants that carry mutations in yield improvement genes, where the mutations increase expression of the yield improvement gene or increase the plant growth and/or organ development activity of the encoded yield improvement polypeptide are provided.

Reducing the Activity and/or Level of a Yield Improvement Polypeptide

Methods are provided to reduce or eliminate the activity of a yield improvement polypeptide of the disclosure by transforming a plant cell with an expression cassette that expresses a polynucleotide that inhibits the expression of the yield improvement polypeptide. The polynucleotide may inhibit the expression of the yield improvement polypeptide directly, by preventing translation of the yield improvement messenger RNA, or indirectly, by encoding a polypeptide that inhibits the transcription or translation of a yield improvement gene encoding a yield improvement polypeptide. Methods for inhibiting or eliminating the expression of a gene in a plant are well known in the art, and any such method may be used in the present disclosure to inhibit the expression of a yield improvement polypeptide.

In accordance with the present disclosure, the expression of a yield improvement polypeptide is inhibited if the protein level of the yield improvement polypeptide is less than 70% of the protein level of the same yield improvement polypeptide in a plant that has not been genetically modified or mutagenized to inhibit the expression of that yield improvement polypeptide. In particular embodiments of the disclosure, the protein level of the yield improvement polypeptide in a modified plant according to the disclosure is less than 60%, less than 50%, less than 40%, less than 30%, less than 20%, less than 10%, less than 5% or less than 2% of the protein level of the same yield improvement polypeptide in a plant that is not a mutant or that has not been genetically modified to inhibit the expression of that yield improvement polypeptide. The expression level of the yield improvement polypeptide may be measured directly, for example, by assaying for the level of yield improvement polypeptide expressed in the plant cell or plant, or indirectly, for example, by measuring the plant growth and/or organ development activity of the yield improvement polypeptide in the plant cell or plant or by measuring the biomass in the plant. Methods for performing such assays are described elsewhere herein.

In other embodiments of the disclosure, the activity of the yield improvement polypeptides is reduced or eliminated by transforming a plant cell with an expression cassette comprising a polynucleotide encoding a polypeptide that inhibits the activity of a yield improvement polypeptide. The plant growth and/or organ development activity of a yield improvement polypeptide is inhibited according to the present disclosure if the plant growth and/or organ development activity of the yield improvement polypeptide is less than 70% of the plant growth and/or organ development activity of the same yield improvement polypeptide in a plant that has not been modified to inhibit the plant growth and/or organ development activity of that yield improvement polypeptide. In particular embodiments of the disclosure, the plant growth and/or organ development activity of the yield improvement polypeptide in a modified plant according to the disclosure is less than 60%, less than 50%, less than 40%, less than 30%, less than 20%, less than 10% or less than 5% of the plant growth and/or organ development activity of the same yield improvement polypeptide in a plant that that has not been modified to inhibit the expression of that yield improvement polypeptide. The plant growth and/or organ development activity of a yield improvement polypeptide is “eliminated” according to the disclosure when it is not detectable by the assay methods described elsewhere herein. Methods of determining the plant growth and/or organ development activity of a yield improvement polypeptide are described elsewhere herein.

In other embodiments, the activity of a yield improvement polypeptide may be reduced or eliminated by disrupting the gene encoding the yield improvement polypeptide. The disclosure encompasses mutagenized plants that carry mutations in yield improvement genes, where the mutations reduce expression of the yield improvement gene or inhibit the plant growth and/or organ development activity of the encoded yield improvement polypeptide.

Thus, many methods may be used to reduce or eliminate the activity of a yield improvement polypeptide. In addition, more than one method may be used to reduce the activity of a single yield improvement polypeptide. Non-limiting examples of methods of reducing or eliminating the expression of yield improvement polypeptides are given below.

1. Polynucleotide-Based Methods:

In some embodiments of the present disclosure, a plant is transformed with an expression cassette that is capable of expressing a polynucleotide that inhibits the expression of a yield improvement polypeptide of the disclosure. The term “expression” as used herein refers to the biosynthesis of a gene product, including the transcription and/or translation of said gene product. For example, for the purposes of the present disclosure, an expression cassette capable of expressing a polynucleotide that inhibits the expression of at least one yield improvement polypeptide is an expression cassette capable of producing an RNA molecule that inhibits the transcription and/or translation of at least one yield improvement polypeptide of the disclosure. The “expression” or “production” of a protein or polypeptide from a DNA molecule refers to the transcription and translation of the coding sequence to produce the protein or polypeptide, while the “expression” or “production” of a protein or polypeptide from an RNA molecule refers to the translation of the RNA coding sequence to produce the protein or polypeptide.

Examples of polynucleotides that inhibit the expression of a yield improvement polypeptide are given below.

i. Sense Suppression/Cosuppression

In some embodiments of the disclosure, inhibition of the expression of a yield improvement polypeptide may be obtained by sense suppression or cosuppression. For cosuppression, an expression cassette is designed to express an RNA molecule corresponding to all or part of a messenger RNA encoding a yield improvement polypeptide in the “sense” orientation. Over expression of the RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the cosuppression expression cassette are screened to identify those that show the greatest inhibition of yield improvement polypeptide expression.

The polynucleotide used for cosuppression may correspond to all or part of the sequence encoding the yield improvement polypeptide, all or part of the 5′ and/or 3′ untranslated region of an yield improvement polypeptide transcript or all or part of both the coding sequence and the untranslated regions of a transcript encoding an yield improvement polypeptide. In some embodiments where the polynucleotide comprises all or part of the coding region for the yield improvement polypeptide, the expression cassette is designed to eliminate the start codon of the polynucleotide so that no protein product will be translated.

Cosuppression may be used to inhibit the expression of plant genes to produce plants having undetectable protein levels for the proteins encoded by these genes. See, for example, Broin, et al., (2002) Plant Cell 14:1417-1432. Cosuppression may also be used to inhibit the expression of multiple proteins in the same plant. See, for example, U.S. Pat. No. 5,942,657. Methods for using cosuppression to inhibit the expression of endogenous genes in plants are described in Flavell, et al., (1994) Proc. Natl. Acad. Sci. USA 91:3490-3496; Jorgensen, et al., (1996) Plant Mol. Biol. 31:957-973; Johansen and Carrington, (2001) Plant Physiol. 126:930-938; Broin, et al., (2002) Plant Cell 14:1417-1432; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Yu, et al., (2003) Phytochemistry 63:753-763 and U.S. Pat. Nos. 5,034,323, 5,283,184 and 5,942,657, each of which is herein incorporated by reference. The efficiency of cosuppression may be increased by including a poly-dT region in the expression cassette at a position 3′ to the sense sequence and 5′ of the polyadenylation signal. See, US Patent Application Publication Number 2002/0048814, herein incorporated by reference. Typically, such a nucleotide sequence has substantial sequence identity to the sequence of the transcript of the endogenous gene, optimally greater than about 65% sequence identity, more optimally greater than about 85% sequence identity, most optimally greater than about 95% sequence identity. See U.S. Pat. Nos. 5,283,184 and 5,034,323, herein incorporated by reference.

ii. Antisense Suppression

In some embodiments of the disclosure, inhibition of the expression of the yield improvement polypeptide may be obtained by antisense suppression. For antisense suppression, the expression cassette is designed to express an RNA molecule complementary to all or part of a messenger RNA encoding the yield improvement polypeptide. Over expression of the antisense RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the antisense suppression expression cassette are screened to identify those that show the greatest inhibition of yield improvement polypeptide expression.

The polynucleotide for use in antisense suppression may correspond to all or part of the complement of the sequence encoding the yield improvement polypeptide, all or part of the complement of the 5′ and/or 3′ untranslated region of the yield improvement transcript or all or part of the complement of both the coding sequence and the untranslated regions of a transcript encoding the yield improvement polypeptide. In addition, the antisense polynucleotide may be fully complementary (i.e., 100% identical to the complement of the target sequence) or partially complementary (i.e., less than 100% identical to the complement of the target sequence) to the target sequence. Antisense suppression may be used to inhibit the expression of multiple proteins in the same plant. See, for example, U.S. Pat. No. 5,942,657. Furthermore, portions of the antisense nucleotides may be used to disrupt the expression of the target gene. Generally, sequences of at least 50 nucleotides, 100 nucleotides, 200 nucleotides, 300, 400, 450, 500, 550 or greater may be used. Methods for using antisense suppression to inhibit the expression of endogenous genes in plants are described, for example, in Liu, et al., (2002) Plant Physiol. 129:1732-1743 and U.S. Pat. Nos. 5,759,829 and 5,942,657, each of which is herein incorporated by reference. Efficiency of antisense suppression may be increased by including a poly-dT region in the expression cassette at a position 3′ to the antisense sequence and 5′ of the polyadenylation signal. See, US Patent Application Publication Number 2002/0048814, herein incorporated by reference.

iii. Double-Stranded RNA Interference

In some embodiments of the disclosure, inhibition of the expression of a yield improvement polypeptide may be obtained by double-stranded RNA (dsRNA) interference. For dsRNA interference, a sense RNA molecule like that described above for cosuppression and an antisense RNA molecule that is fully or partially complementary to the sense RNA molecule are expressed in the same cell, resulting in inhibition of the expression of the corresponding endogenous messenger RNA.

Expression of the sense and antisense molecules can be accomplished by designing the expression cassette to comprise both a sense sequence and an antisense sequence. Alternatively, separate expression cassettes may be used for the sense and antisense sequences. Multiple plant lines transformed with the dsRNA interference expression cassette or expression cassettes are then screened to identify plant lines that show the greatest inhibition of yield improvement polypeptide expression. Methods for using dsRNA interference to inhibit the expression of endogenous plant genes are described in Waterhouse, et al., (1998) Proc. Natl. Acad. Sci. USA 95:13959-13964, Liu, et al., (2002) Plant Physiol. 129:1732-1743 and WO 1999/49029, WO 1999/53050, WO 1999/61631 and WO 2000/49035, each of which is herein incorporated by reference.

iv. Hairpin RNA Interference and Intron-Containing Hairpin RNA Interference

In some embodiments of the disclosure, inhibition of the expression of one or a yield improvement polypeptide may be obtained by hairpin RNA (hpRNA) interference or intron-containing hairpin RNA (ihpRNA) interference. These methods are highly efficient at inhibiting the expression of endogenous genes. See, Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 4:29-38 and the references cited therein.

For hpRNA interference, the expression cassette is designed to express an RNA molecule that hybridizes with itself to form a hairpin structure that comprises a single-stranded loop region and a base-paired stem. The base-paired stem region comprises a sense sequence corresponding to all or part of the endogenous messenger RNA encoding the gene whose expression is to be inhibited, and an antisense sequence that is fully or partially complementary to the sense sequence. Thus, the base-paired stem region of the molecule generally determines the specificity of the RNA interference. hpRNA molecules are highly efficient at inhibiting the expression of endogenous genes, and the RNA interference they induce is inherited by subsequent generations of plants. See, for example, Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:4985-4990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731 and Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 4:29-38. Methods for using hpRNA interference to inhibit or silence the expression of genes are described, for example, in Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:4985-4990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 4:29-38; Pandolfini, et al., BMC Biotechnology 3:7 and US Patent Application Publication Number 2003/0175965, each of which is herein incorporated by reference. A transient assay for the efficiency of hpRNA constructs to silence gene expression in vivo has been described by Panstruga, et al., (2003) Mol. Biol. Rep. 30:135-140, herein incorporated by reference.

For ihpRNA, the interfering molecules have the same general structure as for hpRNA, but the RNA molecule additionally comprises an intron that is capable of being spliced in the cell in which the ihpRNA is expressed. The use of an intron minimizes the size of the loop in the hairpin RNA molecule following splicing, and this increases the efficiency of interference. See, for example, Smith, et al., (2000) Nature 407:319-320. In fact, Smith, et al., show 100% suppression of endogenous gene expression using ihpRNA-mediated interference. Methods for using ihpRNA interference to inhibit the expression of endogenous plant genes are described, for example, in Smith, et al., (2000) Nature 407:319-320; Wesley, et al., (2001) Plant J. 27:581-590; Wang and Waterhouse, (2001) Curr. Opin. Plant Biol. 5:146-150; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 4:29-38; Helliwell and Waterhouse, (2003) Methods 30:289-295 and US Patent Application Publication Number 2003/0180945, each of which is herein incorporated by reference.

The expression cassette for hpRNA interference may also be designed such that the sense sequence and the antisense sequence do not correspond to an endogenous RNA. In this embodiment, the sense and antisense sequence flank a loop sequence that comprises a nucleotide sequence corresponding to all or part of the endogenous messenger RNA of the target gene. Thus, it is the loop region that determines the specificity of the RNA interference. See, for example, WO 2002/00904, herein incorporated by reference.

v. Amplicon-Mediated Interference

Amplicon expression cassettes comprise a plant virus-derived sequence that contains all or part of the target gene but generally not all of the genes of the native virus. The viral sequences present in the transcription product of the expression cassette allow the transcription product to direct its own replication. The transcripts produced by the amplicon may be either sense or antisense relative to the target sequence (i.e., the messenger RNA for the yield improvement polypeptide). Methods of using amplicons to inhibit the expression of endogenous plant genes are described, for example, in Angell and Baulcombe, (1997) EMBO J. 16:3675-3684, Angell and Baulcombe, (1999) Plant J. 20:357-362 and U.S. Pat. No. 6,646,805, each of which is herein incorporated by reference.

vi. Ribozymes

In some embodiments, the polynucleotide expressed by the expression cassette of the disclosure is catalytic RNA or has ribozyme activity specific for the messenger RNA of the yield improvement polypeptide. Thus, the polynucleotide causes the degradation of the endogenous messenger RNA, resulting in reduced expression of the yield improvement polypeptide. This method is described, for example, in U.S. Pat. No. 4,987,071, herein incorporated by reference.

vii. Small Interfering RNA or Micro RNA

In some embodiments of the disclosure, inhibition of the expression of a yield improvement polypeptide may be obtained by RNA interference by expression of a gene encoding a micro RNA (miRNA). miRNAs are regulatory agents consisting of about 22 ribonucleotides. miRNA are highly efficient at inhibiting the expression of endogenous genes. See, for example, Javier, et al., (2003) Nature 425:257-263, herein incorporated by reference.

For miRNA interference, the expression cassette is designed to express an RNA molecule that is modeled on an endogenous miRNA gene. The miRNA gene encodes an RNA that forms a hairpin structure containing a circa 22-nucleotide sequence that is complementary to another endogenous gene (target sequence). For suppression of yield improvement expression, the 22-nucleotide sequence is selected from a yield improvement transcript sequence and contains 22 nucleotides of said yield improvement sequence in sense orientation and 21 nucleotides of a corresponding antisense sequence that is complementary to the sense sequence. miRNA molecules are highly efficient at inhibiting the expression of endogenous genes and the RNA interference they induce is inherited by subsequent generations of plants.

2. Polypeptide-Based Inhibition of Gene Expression

In one embodiment, the polynucleotide encodes a zinc finger protein that binds to a gene encoding a yield improvement polypeptide, resulting in reduced expression of the gene. In particular embodiments, the zinc finger protein binds to a regulatory region of a yield improvement gene. In other embodiments, the zinc finger protein binds to a messenger RNA encoding a yield improvement polypeptide and prevents its translation. Methods of selecting sites for targeting by zinc finger proteins have been described, for example, in U.S. Pat. No. 6,453,242, and methods for using zinc finger proteins to inhibit the expression of genes in plants are described, for example, in US Patent Application Publication Number 2003/0037355, each of which is herein incorporated by reference.

3. Polypeptide-Based Inhibition of Protein Activity

In some embodiments of the disclosure, the polynucleotide encodes an antibody that binds to at least one yield improvement polypeptide and reduces the cell number regulator activity of the yield improvement polypeptide. In another embodiment, the binding of the antibody results in increased turnover of the antibody-yield improvement complex by cellular quality control mechanisms. The expression of antibodies in plant cells and the inhibition of molecular pathways by expression and binding of antibodies to proteins in plant cells are well known in the art. See, for example, Conrad and Sonnewald, (2003) Nature Biotech. 21:35-36, incorporated herein by reference.

4. Gene Disruption

In some embodiments of the present disclosure, the activity of an yield improvement polypeptide is reduced or eliminated by disrupting the gene encoding the yield improvement polypeptide. The gene encoding the yield improvement polypeptide may be disrupted by any method known in the art. For example, in one embodiment, the gene is disrupted by transposon tagging. In another embodiment, the gene is disrupted by mutagenizing plants using random or targeted mutagenesis and selecting for plants that have reduced cell number regulator activity.

i. Transposon Tagging

In one embodiment of the disclosure, transposon tagging is used to reduce or eliminate the yield improvement activity of one or more yield improvement polypeptide. Transposon tagging comprises inserting a transposon within an endogenous yield improvement gene to reduce or eliminate expression of the yield improvement polypeptide. “yield improvement gene” is intended to mean the gene that encodes a yield improvement polypeptide according to the disclosure.

In this embodiment, the expression of one or more yield improvement polypeptide is reduced or eliminated by inserting a transposon within a regulatory region or coding region of the gene encoding the yield improvement polypeptide. A transposon that is within an exon, intron, 5′ or 3′ untranslated sequence, a promoter or any other regulatory sequence of a yield improvement gene may be used to reduce or eliminate the expression and/or activity of the encoded yield improvement polypeptide.

Methods for the transposon tagging of specific genes in plants are well known in the art. See, for example, Maes, et al., (1999) Trends Plant Sci. 4:90-96; Dharmapuri and Sonti, (1999) FEMS Microbiol. Lett. 179:53-59; Meissner, et al., (2000) Plant J. 22:265-274; Phogat, et al., (2000) J. Biosci. 25:57-63; Walbot, (2000) Curr. Opin. Plant Biol. 2:103-107; Gai, et al., (2000) Nucleic Acids Res. 28:94-96; Fitzmaurice, et al., (1999) Genetics 153:1919-1928). In addition, the TUSC process for selecting Mu insertions in selected genes has been described in Bensen, et al., (1995) Plant Cell 7:75-84; Mena, et al., (1996) Science 274:1537-1540 and U.S. Pat. No. 5,962,764, each of which is herein incorporated by reference.

ii. Mutant Plants with Reduced Activity

Additional methods for decreasing or eliminating the expression of endogenous genes in plants are also known in the art and can be similarly applied to the instant disclosure. These methods include other forms of mutagenesis, such as ethyl methanesulfonate-induced mutagenesis, deletion mutagenesis and fast neutron deletion mutagenesis used in a reverse genetics sense (with PCR) to identify plant lines in which the endogenous gene has been deleted. For examples of these methods see, Ohshima, et al., (1998) Virology 243:472-481; Okubara, et al., (1994) Genetics 137:867-874 and Quesada, et al., (2000) Genetics 154:421-436, each of which is herein incorporated by reference. In addition, a fast and automatable method for screening for chemically induced mutations, TILLING (Targeting Induced Local Lesions In Genomes), using denaturing HPLC or selective endonuclease digestion of selected PCR products is also applicable to the instant disclosure. See, McCallum, et al., (2000) Nat. Biotechnol. 18:455-457, herein incorporated by reference.

Mutations that impact gene expression or that interfere with the function (cell number regulator activity) of the encoded protein are well known in the art. Insertional mutations in gene exons usually result in null-mutants. Mutations in conserved residues are particularly effective in inhibiting the cell number regulator activity of the encoded protein. Conserved residues of nutrient update improvement polypeptides suitable for mutagenesis with the goal to eliminate cell number regulator activity have been described. Such mutants can be isolated according to well-known procedures, and mutations in different yield improvement loci can be stacked by genetic crossing. See, for example, Gruis, et al., (2002) Plant Cell 14:2863-2882.

In another embodiment of this disclosure, dominant mutants can be used to trigger RNA silencing due to gene inversion and recombination of a duplicated gene locus. See, for example, Kusaba, et al., (2003) Plant Cell 15:1455-1467.

The disclosure encompasses additional methods for reducing or eliminating the activity of one or more yield improvement polypeptide. Examples of other methods for altering or mutating a genomic nucleotide sequence in a plant are known in the art and include, but are not limited to, the use of RNA:DNA vectors, RNA:DNA mutational vectors, RNA:DNA repair vectors, mixed-duplex oligonucleotides, self-complementary RNA:DNA oligonucleotides and recombinogenic oligonucleobases. Such vectors and methods of use are known in the art. See, for example, U.S. Pat. Nos. 5,565,350; 5,731,181; 5,756,325; 5,760,012; 5,795,972 and 5,871,984, each of which are herein incorporated by reference. See also, WO 1998/49350, WO 1999/07865, WO 1999/25821 and Beetham, et al., (1999) Proc. Natl. Acad. Sci. USA 96:8774-8778, each of which is herein incorporated by reference.

iii. Modulating Plant Growth and/or Organ Development Activity

In specific methods, the level and/or activity of a cell number regulator in a plant is increased by increasing the level or activity of the yield improvement polypeptide in the plant. Methods for increasing the level and/or activity of yield improvement polypeptides in a plant are discussed elsewhere herein. Briefly, such methods comprise providing a yield improvement polypeptide of the disclosure to a plant and thereby increasing the level and/or activity of the yield improvement polypeptide. In other embodiments, an yield improvement nucleotide sequence encoding an yield improvement polypeptide can be provided by introducing into the plant a polynucleotide comprising an yield improvement nucleotide sequence of the disclosure, expressing the yield improvement sequence, increasing the activity of the yield improvement polypeptide and thereby increasing the number of tissue cells in the plant or plant part. In other embodiments, the yield improvement nucleotide construct introduced into the plant is stably incorporated into the genome of the plant.

In other methods, the number of cells and biomass of a plant tissue is increased by increasing the level and/or activity of the yield improvement polypeptide in the plant. Such methods are disclosed in detail elsewhere herein. In one such method, a yield improvement nucleotide sequence is introduced into the plant and expression of said yield improvement nucleotide sequence decreases the activity of the yield improvement polypeptide and thereby increasing the plant growth and/or organ development in the plant or plant part. In other embodiments, the yield improvement nucleotide construct introduced into the plant is stably incorporated into the genome of the plant.

As discussed above, one of skill will recognize the appropriate promoter to use to modulate the level/activity of a plant growth and/or organ development polynucleotide and polypeptide in the plant. Exemplary promoters for this embodiment have been disclosed elsewhere herein.

Accordingly, the present disclosure further provides plants having a modified plant growth and/or organ development when compared to the plant growth and/or organ development of a control plant tissue. In one embodiment, the plant of the disclosure has an increased level/activity of the yield improvement polypeptide of the disclosure and thus has increased plant growth and/or organ development in the plant tissue. In other embodiments, the plant of the disclosure has a reduced or eliminated level of the yield improvement polypeptide of the disclosure and thus has decreased plant growth and/or organ development in the plant tissue. In other embodiments, such plants have stably incorporated into their genome a nucleic acid molecule comprising a yield improvement nucleotide sequence of the disclosure operably linked to a promoter that drives expression in the plant cell.

iv. Modulating Root Development

Methods for modulating root development in a plant are provided. By “modulating root development” is intended any alteration in the development of the plant root when compared to a control plant. Such alterations in root development include, but are not limited to, alterations in the growth rate of the primary root, the fresh root weight, the extent of lateral and adventitious root formation, the vasculature system, meristem development or radial expansion.

Methods for modulating root development in a plant are provided. The methods comprise modulating the level and/or activity of the yield improvement polypeptide in the plant. In one method, a yield improvement sequence of the disclosure is provided to the plant. In another method, the yield improvement nucleotide sequence is provided by introducing into the plant a polynucleotide comprising a yield improvement nucleotide sequence of the disclosure, expressing the yield improvement sequence and thereby modifying root development. In still other methods, the yield improvement nucleotide construct introduced into the plant is stably incorporated into the genome of the plant.

In other methods, root development is modulated by altering the level or activity of the yield improvement polypeptide in the plant. An increase in yield improvement activity can result in at least one or more of the following alterations to root development, including, but not limited to, larger root meristems, increased in root growth, enhanced radial expansion, an enhanced vasculature system, increased root branching, more adventitious roots and/or an increase in fresh root weight when compared to a control plant.

As used herein, “root growth” encompasses all aspects of growth of the different parts that make up the root system at different stages of its development in both monocotyledonous and dicotyledonous plants. It is to be understood that enhanced root growth can result from enhanced growth of one or more of its parts including the primary root, lateral roots, adventitious roots, etc.

Methods of measuring such developmental alterations in the root system are known in the art. See, for example, US Patent Application Publication Number 2003/0074698 and Werner, et al., (2001) PNAS 18:10487-10492, both of which are herein incorporated by reference.

As discussed above, one of skill will recognize the appropriate promoter to use to modulate root development in the plant. Exemplary promoters for this embodiment include constitutive promoters and root-preferred promoters. Exemplary root-preferred promoters have been disclosed elsewhere herein.

Stimulating root growth and increasing root mass by increasing the activity and/or level of the yield improvement polypeptide also finds use in improving the standability of a plant. The term “resistance to lodging” or “standability” refers to the ability of a plant to fix itself to the soil. For plants with an erect or semi-erect growth habit, this term also refers to the ability to maintain an upright position under adverse (environmental) conditions. This trait relates to the size, depth and morphology of the root system. In addition, stimulating root growth and increasing root mass by increasing the level and/or activity of the yield improvement polypeptide also finds use in promoting in vitro propagation of explants.

Furthermore, higher root biomass production due to an increased level and/or activity of yield improvement activity has a direct effect on the yield and an indirect effect of production of compounds produced by root cells or transgenic root cells or cell cultures of said transgenic root cells. One example of an interesting compound produced in root cultures is shikonin, the yield of which can be advantageously enhanced by said methods.

Accordingly, the present disclosure further provides plants having modulated root development when compared to the root development of a control plant. In some embodiments, the plant of the disclosure has an increased level/activity of the yield improvement polypeptide of the disclosure and has enhanced root growth and/or root biomass. In other embodiments, such plants have stably incorporated into their genome a nucleic acid molecule comprising a yield improvement nucleotide sequence of the disclosure operably linked to a promoter that drives expression in the plant cell.

v. Modulating Shoot and Leaf Development

Methods are also provided for modulating shoot and leaf development in a plant. By “modulating shoot and/or leaf development” is intended any alteration in the development of the plant shoot and/or leaf. Such alterations in shoot and/or leaf development include, but are not limited to, alterations in shoot meristem development, in leaf number, leaf size, leaf and stem vasculature, internode length and leaf senescence. As used herein, “leaf development” and “shoot development” encompasses all aspects of growth of the different parts that make up the leaf system and the shoot system, respectively, at different stages of their development, both in monocotyledonous and dicotyledonous plants. Methods for measuring such developmental alterations in the shoot and leaf system are known in the art. See, for example, Werner, et al., (2001) PNAS 98:10487-10492 and US Patent Application Publication Number 2003/0074698, each of which is herein incorporated by reference.

The method for modulating shoot and/or leaf development in a plant comprises modulating the activity and/or level of a yield improvement polypeptide of the disclosure. In one embodiment, a yield improvement sequence of the disclosure is provided. In other embodiments, the yield improvement nucleotide sequence can be provided by introducing into the plant a polynucleotide comprising a yield improvement nucleotide sequence of the disclosure, expressing the yield improvement sequence, and thereby modifying shoot and/or leaf development. In other embodiments, the yield improvement nucleotide construct introduced into the plant is stably incorporated into the genome of the plant.

In specific embodiments, shoot or leaf development is modulated by decreasing the level and/or activity of the yield improvement polypeptide in the plant. An decrease in yield improvement activity can result in at least one or more of the following alterations in shoot and/or leaf development, including, but not limited to, reduced leaf number, reduced leaf surface, reduced vascular, shorter internodes and stunted growth and retarded leaf senescence, when compared to a control plant.

As discussed above, one of skill will recognize the appropriate promoter to use to modulate shoot and leaf development of the plant. Exemplary promoters for this embodiment include constitutive promoters, shoot-preferred promoters, shoot meristem-preferred promoters and leaf-preferred promoters. Exemplary promoters have been disclosed elsewhere herein.

Decreasing yield improvement activity and/or level in a plant results in shorter internodes and stunted growth. Thus, the methods of the disclosure find use in producing dwarf plants. In addition, as discussed above, modulations of yield improvement activity in the plant modulates both root and shoot growth. Thus, the present disclosure further provides methods for altering the root/shoot ratio. Shoot or leaf development can further be modulated by decreasing the level and/or activity of the yield improvement polypeptide in the plant.

Accordingly, the present disclosure further provides plants having modulated shoot and/or leaf development when compared to a control plant. In some embodiments, the plant of the disclosure has an increased level/activity of the yield improvement polypeptide of the disclosure, altering the shoot and/or leaf development. Such alterations include, but are not limited to, increased leaf number, increased leaf surface, increased vascularity, longer internodes and increased plant stature, as well as alterations in leaf senescence, as compared to a control plant. In other embodiments, the plant of the disclosure has a decreased level/activity of the yield improvement polypeptide of the disclosure.

vi Modulating Reproductive Tissue Development

Methods for modulating reproductive tissue development are provided. In one embodiment, methods are provided to modulate floral development in a plant. By “modulating floral development” is intended any alteration in a structure of a plant's reproductive tissue as compared to a control plant in which the activity or level of the yield improvement polypeptide has not been modulated. “Modulating floral development” further includes any alteration in the timing of the development of a plant's reproductive tissue (i.e., a delayed or an accelerated timing of floral development) when compared to a control plant in which the activity or level of the yield improvement polypeptide has not been modulated. Macroscopic alterations may include changes in size, shape, number or location of reproductive organs, the developmental time period that these structures form or the ability to maintain or proceed through the flowering process in times of environmental stress. Microscopic alterations may include changes to the types or shapes of cells that make up the reproductive organs.

The method for modulating floral development in a plant comprises modulating yield improvement activity in a plant. In one method, a yield improvement sequence of the disclosure is provided. A yield improvement nucleotide sequence can be provided by introducing into the plant a polynucleotide comprising a yield improvement nucleotide sequence of the disclosure, expressing the yield improvement sequence and thereby modifying floral development. In other embodiments, the yield improvement nucleotide construct introduced into the plant is stably incorporated into the genome of the plant.

In specific methods, floral development is modulated by decreasing the level or activity of the yield improvement polypeptide in the plant. A decrease in yield improvement activity can result in at least one or more of the following alterations in floral development, including, but not limited to, retarded flowering, reduced number of flowers, partial male sterility and reduced seed set, when compared to a control plant. Inducing delayed flowering or inhibiting flowering can be used to enhance yield in forage crops such as alfalfa. Methods for measuring such developmental alterations in floral development are known in the art. See, for example, Mouradov, et al., (2002) The Plant Cell S111-S130, herein incorporated by reference.

As discussed above, one of skill will recognize the appropriate promoter to use to modulate floral development of the plant. Exemplary promoters for this embodiment include constitutive promoters, inducible promoters, shoot-preferred promoters and inflorescence-preferred promoters.

In other methods, floral development is modulated by increasing the level and/or activity of the yield improvement sequence of the disclosure. Such methods can comprise introducing a yield improvement nucleotide sequence into the plant and increasing the activity of the yield improvement polypeptide. In other methods, the yield improvement nucleotide construct introduced into the plant is stably incorporated into the genome of the plant. Increasing expression of the yield improvement sequence of the disclosure can modulate floral development during periods of stress. Such methods are described elsewhere herein. Accordingly, the present disclosure further provides plants having modulated floral development when compared to the floral development of a control plant. Compositions include plants having an increased level/activity of the yield improvement polypeptide of the disclosure and having an altered floral development. Compositions also include plants having an increased level/activity of the yield improvement polypeptide of the disclosure wherein the plant maintains or proceeds through the flowering process in times of stress.

Methods are also provided for the use of the yield improvement sequences of the disclosure to increase seed size and/or weight. The method comprises increasing the activity of the yield improvement sequences in a plant or plant part, such as the seed. An increase in seed size and/or weight comprises an increased size or weight of the seed and/or an increase in the size or weight of one or more seed parts including, for example, the embryo, endosperm, seed coat, aleurone or cotyledon.

As discussed above, one of skill will recognize the appropriate promoter to use to increase seed size and/or seed weight. Exemplary promoters of this embodiment include constitutive promoters, inducible promoters, seed-preferred promoters, embryo-preferred promoters and endosperm-preferred promoters.

The method for decreasing seed size and/or seed weight in a plant comprises decreasing yield improvement activity in the plant. In one embodiment, the yield improvement nucleotide sequence can be provided by introducing into the plant a polynucleotide comprising a yield improvement nucleotide sequence of the disclosure, expressing the yield improvement sequence, and thereby decreasing seed weight and/or size. In other embodiments, the yield improvement nucleotide construct introduced into the plant is stably incorporated into the genome of the plant.

It is further recognized that increasing seed size and/or weight can also be accompanied by an increase in the speed of growth of seedlings or an increase in early vigor. As used herein, the term “early vigor” refers to the ability of a plant to grow rapidly during early development, and relates to the successful establishment, after germination, of a well-developed root system and a well-developed photosynthetic apparatus. In addition, an increase in seed size and/or weight can also result in an increase in nutrient update when compared to a control.

Accordingly, the present disclosure further provides plants having an increased seed weight and/or seed size when compared to a control plant. In other embodiments, plants having an increased vigor and nutrient update are also provided. In some embodiments, the plant of the disclosure has an increased level/activity of the yield improvement polypeptide of the disclosure and has an increased seed weight and/or seed size. In other embodiments, such plants have stably incorporated into their genome a nucleic acid molecule comprising a yield improvement nucleotide sequence of the disclosure operably linked to a promoter that drives expression in the plant cell.

vii. Method of Use for Yield Improvement Promoter Polynucleotides

The polynucleotides comprising the yield improvement promoters disclosed in the present disclosure, as well as variants and fragments thereof, are useful in the genetic manipulation of any host cell, preferably plant cell, when assembled with a DNA construct such that the promoter sequence is operably linked to a nucleotide sequence comprising a polynucleotide of interest. In this manner, the yield improvement promoter polynucleotides of the disclosure are provided in expression cassettes along with a polynucleotide sequence of interest for expression in the host cell of interest. As discussed in Example 2 below, the yield improvement promoter sequences of the disclosure are expressed in a variety of tissues and thus the promoter sequences can find use in regulating the temporal and/or the spatial expression of polynucleotides of interest.

Synthetic hybrid promoter regions are known in the art. Such regions comprise upstream promoter elements of one polynucleotide operably linked to the promoter element of another polynucleotide. In an embodiment of the disclosure, heterologous sequence expression is controlled by a synthetic hybrid promoter comprising the yield improvement promoter sequences of the disclosure, or a variant or fragment thereof, operably linked to upstream promoter element(s) from a heterologous promoter. Upstream promoter elements that are involved in the plant defense system have been identified and may be used to generate a synthetic promoter. See, for example, Rushton, et al., (1998) Curr. Opin. Plant Biol. 1:311-315. Alternatively, a synthetic yield improvement promoter sequence may comprise duplications of the upstream promoter elements found within the yield improvement promoter sequences.

It is recognized that the promoter sequence of the disclosure may be used with its native yield improvement coding sequences. A DNA construct comprising the yield improvement promoter operably linked with its native yield improvement gene may be used to transform any plant of interest to bring about a desired phenotypic change, such as modulating cell number, modulating root, shoot, leaf, floral and embryo development, stress tolerance and any other phenotype described elsewhere herein.

The promoter nucleotide sequences and methods disclosed herein are useful in regulating expression of any heterologous nucleotide sequence in a host plant in order to vary the phenotype of a plant. Various changes in phenotype are of interest including modifying the fatty acid composition in a plant, altering the amino acid content of a plant, altering a plant's pathogen defense mechanism, and the like. These results can be achieved by providing expression of heterologous products or increased expression of endogenous products in plants. Alternatively, the results can be achieved by providing for a reduction of expression of one or more endogenous products, particularly enzymes or cofactors in the plant. These changes result in a change in phenotype of the transformed plant.

Genes of interest are reflective of the commercial markets and interests of those involved in the development of the crop. Crops and markets of interest change, and as developing nations open up world markets, new crops and technologies will emerge also. In addition, as our understanding of agronomic traits and characteristics such as yield and heterosis increase, the choice of genes for transformation will change accordingly. General categories of genes of interest include, for example, those genes involved in information, such as zinc fingers, those involved in communication, such as kinases and those involved in housekeeping, such as heat shock proteins. More specific categories of transgenes, for example, include genes encoding important traits for agronomics, insect resistance, disease resistance, herbicide resistance, sterility, grain characteristics and commercial products. Genes of interest include, generally, those involved in oil, starch, carbohydrate or nutrient metabolism as well as those affecting kernel size, sucrose loading, and the like.

In certain embodiments the nucleic acid sequences of the present disclosure can be used in combination (“stacked”) with other polynucleotide sequences of interest in order to create plants with a desired phenotype. The combinations generated can include multiple copies of any one or more of the polynucleotides of interest. The polynucleotides of the present disclosure may be stacked with any gene or combination of genes to produce plants with a variety of desired trait combinations, including but not limited to traits desirable for animal feed such as high oil genes (e.g., U.S. Pat. No. 6,232,529); balanced amino acids (e.g., hordothionins (U.S. Pat. Nos. 5,990,389; 5,885,801; 5,885,802 and 5,703,409); barley high lysine (Williamson, et al., (1987) Eur. J. Biochem. 165:99-106 and WO 1998/20122) and high methionine proteins (Pedersen, et al., (1986) J. Biol. Chem. 261:6279; Kirihara, et al., (1988) Gene 71:359 and Musumura, et al., (1989) Plant Mol. Biol. 12:123)); increased digestibility (e.g., modified storage proteins (U.S. patent application Ser. No. 10/053,410, filed Nov. 7, 2001) and thioredoxins (U.S. patent application Ser. No. 10/005,429, filed Dec. 3, 2001)), the disclosures of which are herein incorporated by reference. The polynucleotides of the present disclosure can also be stacked with traits desirable for insect, disease or herbicide resistance (e.g., Bacillus thuringiensis toxic proteins (U.S. Pat. Nos. 5,366,892; 5,747,450; 5,737,514; 5,723,756; 5,593,881; Geiser, et al., (1986) Gene 48:109); lectins (Van Damme, et al., (1994) Plant Mol. Biol. 24:825); fumonisin detoxification genes (U.S. Pat. No. 5,792,931); avirulence and disease resistance genes (Jones, et al., (1994) Science 266:789; Martin, et al., (1993) Science 262:1432; Mindrinos, et al., (1994) Cell 78:1089); acetolactate synthase (ALS) mutants that lead to herbicide resistance such as the S4 and/or Hra mutations; inhibitors of glutamine synthase such as phosphinothricin or basta (e.g., bar gene) and glyphosate resistance (EPSPS gene)) and traits desirable for processing or process products such as high oil (e.g., U.S. Pat. No. 6,232,529); modified oils (e.g., fatty acid desaturase genes (U.S. Pat. No. 5,952,544; WO 94/11516)); modified starches (e.g., ADPG pyrophosphorylases (AGPase), starch synthases (SS), starch branching enzymes (SBE) and starch debranching enzymes (SDBE)) and polymers or bioplastics (e.g., U.S. Pat. No. 5,602,321; beta-ketothiolase, polyhydroxybutyrate synthase and acetoacetyl-CoA reductase (Schubert, et al., (1988) J. Bacteriol. 170:5837-5847) facilitate expression of polyhydroxyalkanoates (PHAs)), the disclosures of which are herein incorporated by reference. One could also combine the polynucleotides of the present disclosure with polynucleotides affecting agronomic traits such as male sterility (e.g., see, U.S. Pat. No. 5,583,210), stalk strength, flowering time or transformation technology traits such as cell cycle regulation or gene targeting (e.g., WO 1999/61619; WO 2000/17364; WO 1999/25821), the disclosures of which are herein incorporated by reference.

In one embodiment, sequences of interest improve plant growth and/or crop yields. For example, sequences of interest include agronomically important genes that result in improved primary or lateral root systems. Such genes include, but are not limited to, nutrient/water transporters and growth induces. Examples of such genes, include but are not limited to, maize plasma membrane H⁺-ATPase (MHA2) (Frias, et al., (1996) Plant Cell 8:1533-44); AKT1, a component of the potassium uptake apparatus in Arabidopsis, (Spalding, et al., (1999) J Gen Physiol 113:909-18); RML genes which activate cell division cycle in the root apical cells (Cheng, et al., (1995) Plant Physiol 108:881); maize glutamine synthetase genes (Sukanya, et al., (1994) Plant Mol Biol 26:1935-46) and hemoglobin (Duff, et al., (1997) J. Biol. Chem 27:16749-16752, Arredondo-Peter, et al., (1997) Plant Physiol. 115:1259-1266; Arredondo-Peter, et al., (1997) Plant Physiol 114:493-500, and references sited therein). The sequence of interest may also be useful in expressing antisense nucleotide sequences of genes that that negatively affects root development.

Additional, agronomically important traits such as oil, starch and protein content can be genetically altered in addition to using traditional breeding methods. Modifications include increasing content of oleic acid, saturated and unsaturated oils, increasing levels of lysine and sulfur, providing essential amino acids and also modification of starch. Hordothionin protein modifications are described in U.S. Pat. Nos. 5,703,049, 5,885,801, 5,885,802 and 5,990,389, herein incorporated by reference. Another example is lysine and/or sulfur rich seed protein encoded by the soybean 2S albumin described in U.S. Pat. No. 5,850,016 and the chymotrypsin inhibitor from barley, described in Williamson, et al., (1987) Eur. J. Biochem. 165:99-106, the disclosures of which are herein incorporated by reference.

Derivatives of the coding sequences can be made by site-directed mutagenesis to increase the level of preselected amino acids in the encoded polypeptide. For example, the gene encoding the barley high lysine polypeptide (BHL) is derived from barley chymotrypsin inhibitor, U.S. patent application Ser. No. 08/740,682, filed Nov. 1, 1996 and WO 1998/20133, the disclosures of which are herein incorporated by reference. Other proteins include methionine-rich plant proteins such as from sunflower seed (Lilley, et al., (1989) Proceedings of the World Congress on Vegetable Protein Utilization in Human Foods and Animal Feedstuffs, ed. Applewhite, (American Oil Chemists Society, Champaign, Ill.), pp. 497-502, herein incorporated by reference); corn (Pedersen, et al., (1986) J. Biol. Chem. 261:6279; Kirihara, et al., (1988) Gene 71:359, both of which are herein incorporated by reference) and rice (Musumura, et al., (1989) Plant Mol. Biol. 12:123, herein incorporated by reference). Other agronomically important genes encode latex, Floury 2, growth factors, seed storage factors and transcription factors.

Insect resistance genes may encode resistance to pests that have great yield drag such as rootworm, cutworm, European Corn Borer, and the like. Such genes include, for example, Bacillus thuringiensis toxic protein genes (U.S. Pat. No. 5,366,892; 5,747,450; 5,736,514; 5,723,756; 5,593,881 and Geiser, et al., (1986) Gene 48:109), and the like.

Genes encoding disease resistance traits include detoxification genes, such as against fumonosin (U.S. Pat. No. 5,792,931); avirulence (avr) and disease resistance (R) genes (Jones, et al., (1994) Science 266:789; Martin, et al., (1993) Science 262:1432 and Mindrinos, et al., (1994) Cell 78:1089), and the like.

Herbicide resistance traits may include genes coding for resistance to herbicides that act to inhibit the action of acetolactate synthase (ALS), in particular the sulfonylurea-type herbicides (e.g., the acetolactate synthase (ALS) gene containing mutations leading to such resistance, in particular the S4 and/or Hra mutations), genes coding for resistance to herbicides that act to inhibit action of glutamine synthase, such as phosphinothricin or basta (e.g., the bar gene) or other such genes known in the art. The bar gene encodes resistance to the herbicide basta, the nptII gene encodes resistance to the antibiotics kanamycin and geneticin and the ALS-gene mutants encode resistance to the herbicide chlorsulfuron.

Sterility genes can also be encoded in an expression cassette and provide an alternative to physical detasseling. Examples of genes used in such ways include male tissue-preferred genes and genes with male sterility phenotypes such as QM, described in U.S. Pat. No. 5,583,210. Other genes include kinases and those encoding compounds toxic to either male or female gametophytic development.

The quality of grain is reflected in traits such as levels and types of oils, saturated and unsaturated, quality and quantity of essential amino acids and levels of cellulose. In corn, modified hordothionin proteins are described in U.S. Pat. Nos. 5,703,049, 5,885,801, 5,885,802 and 5,990,389.

Commercial traits can also be encoded on a gene or genes that could increase for example, starch for ethanol production, or provide expression of proteins. Another important commercial use of transformed plants is the production of polymers and bioplastics such as described in U.S. Pat. No. 5,602,321. Genes such as β-Ketothiolase, PHBase (polyhydroxyburyrate synthase) and acetoacetyl-CoA reductase (see, Schubert, et al., (1988) J. Bacteriol. 170:5837-5847) facilitate expression of polyhyroxyalkanoates (PHAs).

Exogenous products include plant enzymes and products as well as those from other sources including procaryotes and other eukaryotes. Such products include enzymes, cofactors, hormones and the like. The level of proteins, particularly modified proteins having improved amino acid distribution to improve the nutrient value of the plant, can be increased. This is achieved by the expression of such proteins having enhanced amino acid content.

When referring to the relationship between two genetic elements, such as a genetic element contributing to tolerance and a proximal marker, “coupling” phase linkage indicates the state where the “favorable” allele at the tolerance locus is physically associated on the same chromosome strand as the “favorable” allele of the respective linked marker locus. In coupling phase, both favorable alleles are inherited together by progeny that inherit that chromosome strand. In “repulsion” phase linkage, the “favorable” allele at the locus of interest (e.g., a QTL for tolerance) is physically linked with an “unfavorable” allele at the proximal marker locus, and the two “favorable” alleles are not inherited together (i.e., the two loci are “out of phase” with each other).

“Linkage disequilibrium” generally refers to a phenomenon wherein alleles tend to remain together in linkage groups when segregating from parents to offspring, with a greater frequency than expected from their individual frequencies.

“Linkage group” generally refers to traits or markers that generally co-segregate. A linkage group generally corresponds to a chromosomal region containing genetic material that encodes the traits or markers. “Locus” refers to a segment of DNA.

A “map location,” “map position” or “relative map position” is an assigned location on a genetic map relative to linked genetic markers where a specified marker can be found within a given species. Map positions are generally provided in centimorgans. A “physical position” or “physical location” is the position, typically in nucleotide bases, of a particular nucleotide, such as a SNP nucleotide, on the chromosome.

“Mapping” is the process of defining the linkage relationships of loci through the use of genetic markers, populations segregating for the markers and standard genetic principles of recombination frequency.

“Marker” or “molecular marker” is a term used to denote a nucleic acid or amino acid sequence that is sufficiently unique to characterize a specific locus on the genome. Any detectible polymorphic trait can be used as a marker so long as it is inherited differentially and exhibits linkage disequilibrium with a phenotypic trait of interest. Each marker is an indicator of a specific segment of DNA, having a unique nucleotide sequence. The map positions provide a measure of the relative positions of particular markers with respect to one another. When a trait is stated to be linked to a given marker, it will be understood that the actual DNA segment whose sequence affects the trait generally co-segregates with the marker. More precise and definite localization of a trait can be obtained if markers are identified on both sides of the trait. By measuring the appearance of the marker(s) in progeny of crosses, the existence of the trait can be detected by relatively simple molecular tests without actually evaluating the appearance of the trait itself, which can be difficult and time-consuming because the actual evaluation of the trait requires growing plants to a stage and/or under environmental conditions where the trait can be expressed. Molecular markers have been widely used to determine genetic composition in crop plants. “Marker assisted selection” refers to the process of selecting a desired trait or traits in a plant or plants by detecting one or more nucleic acids from the plant, where the nucleic acid is linked to the desired trait, and then selecting the plant or germplasm possessing those one or more nucleic acids.

“Haplotype” generally refers to a combination of particular alleles present within a particular plant's genome at two or more linked marker loci, for instance at two or more loci on a particular linkage group.

“Polymorphism” means a change or difference between two related nucleic acids. A “nucleotide polymorphism” refers to a nucleotide that is different in one sequence when compared to a related sequence when the two nucleic acids are aligned for maximal correspondence.

“Quantitative trait loci” or “QTL” refer to the genetic elements controlling a quantitative trait.

Provided are markers and haplotypes associated with tolerance of abiotic to root-knot nematode, as well as related primers and/or probes and methods for the use of any of the foregoing for identifying and/or selecting soybean plants with improved tolerance to root-knot nematode. A method for determining the presence or absence of at least one allele of a particular marker or haplotype associated with tolerance to root-knot nematode comprises analyzing genomic DNA from a soybean plant or germplasm to determine if at least one, or a plurality, of such markers is present or absent and if present, determining the allelic form of the marker(s). If a plurality of markers on a single linkage group are investigated, this information regarding the markers present in the particular plant or germplasm can be used to determine a haplotype for that plant/germplasm.

This disclosure can be better understood by reference to the following non-limiting examples. It will be appreciated by those skilled in the art that other embodiments of the disclosure may be practiced without departing from the spirit and the scope of the disclosure as herein disclosed and claimed.

EXAMPLES
Example 1
Identification of Sequences of Interest

A multi-faceted computational analysis was done to identify a set of genes that can improve crop yield. The yield enhancement may occur through various physiological avenues, but especially via drought tolerance or WUE efficiency. These genes comprised a set of 1703 genes. These genes were identified by analyses relying on multiple sets of profiling data, pathway-network curation and literature interpretation. Most of the genes hail from sorghum, which is known to be a drought tolerant crop and many have root or root-preferred expression. This work consisted of several substeps, including: Part_—1. Generate sorghum orthologs for genes already in the testing pipeline as well as newly nominated genes slated for that pipeline. Part_—2. Literature and Nominations. A set of genes from literature were identified, and also a complex search of proprietary software that intersects various genomic and genetic information was used to generate a subset of genes of interest. Part_—3. Sorghum Profiling Analyses, especially emphasizing sorghum genes that are stress/drought responsive where the maize orthologs are not. Part_—4. Sorghum orthologs to maize mRNA profiling results of a proprietary set of elite germplasm tested under well-watered and drought conditions where this set of genes correlated to yield performance. These were dubbed yield stability genes, with the stability being under drought. Part_—5. Root Hair Specific Set. As set of sorghum orthologs were identified to the Arabidopsis root hair formation genes. All the genes from parts 1-5 were gathered, sequence redundancy removed and they were further filtered by whether the ORF was complete and the degree of sorghum root preference in expression.

Example 2
Transgenic FAST Corn

Transgenic FAST Corn plants transformed with three sorghum genes expressed from the constitutive ubiquitin promoter from maize were subjected to a reproductive drought screen at the T1 generation. The three constructs, Sb09g004150, Sb03g011680 and Sb06g033870, were selected for the T1 reproductive drought evaluation based on phenomic data from T0 FAST Corn plants. TO phenotyping involves measurement of overall growth of the plant as well as measurement of yield components. T1 reproductive drought assay involves imposition of a chronic drought stress starting at the vegetative stage and continuing through to the flowering stage. The experiment is terminated prior to grain filling, at 8 days after silking and the reproductive parameters including ear area, ear length, ear width and silk count are determined.

Evaluation of TO plants of Sb09g004150 indicated that 3 out 10 tested events had statistically significant increase in ear area and maximum total plant area. At the construct level, several traits were statistically significant on the positive side, and these traits include ear area, ear length, ear width, maximum total plant area and seed number. In the T1 reproductive drought assay, 6 events were evaluated, and some parameters were positive and some negative amongst these events. T0 plants of Sb03g011680 showed significantly positive maximum total plant area for 2 of 10 events. T1 assay under drought for 6 events of this construct revealed two events with significantly improved ear area of which one had significantly increased ear length as well. In the case of Sb06g033870, 4 of 10 events evaluated at the T0 stage had significantly positive ear area and three had significantly positive seed number as well. At the construct level, ear area, ear length, maximum total plant area and seed number were all significantly positive. This construct, when tested in the T1 reproductive assay, showed one of six events with significantly positive ear area, ear length and silk count. The anthesis silking interval was significantly high for this event as well.

Example 3
Transformation and Regeneration of Transgenic Plants

Immature maize embryos from greenhouse donor plants are bombarded with a plasmid containing the sorghum uptake or stress tolerance sequence operably linked to the drought-inducible promoter RAB17 promoter (Vilardell, et al., (1990) Plant Mol Biol 14:423-432) and the selectable marker gene PAT, which confers resistance to the herbicide Bialaphos. Alternatively, the selectable marker gene is provided on a separate plasmid. Transformation is performed as follows. Media recipes follow below.

Preparation of Target Tissue:

The ears are husked and surface sterilized in 30% Clorox® bleach plus 0.5% Micro detergent for 20 minutes and rinsed two times with sterile water. The immature embryos are excised and placed embryo axis side down (scutellum side up), 25 embryos per plate, on 560Y medium for 4 hours and then aligned within the 2.5-cm target zone in preparation for bombardment.

Preparation of DNA:

A plasmid vector comprising the nutrient uptake/stress tolerance sequence operably linked to an ubiquitin promoter is made. This plasmid DNA plus plasmid DNA containing a PAT selectable marker is precipitated onto 1.1 μm (average diameter) tungsten pellets using a CaCl₂precipitation procedure as follows:

100 μl prepared tungsten particles in water

10 μl (1 μg) DNA in Tris EDTA buffer (1 μg total DNA)

100 μl 2.5 M CaCl₂

10 μl 0.1 M spermidine

Each reagent is added sequentially to the tungsten particle suspension, while maintained on the multitube vortexer. The final mixture is sonicated briefly and allowed to incubate under constant vortexing for 10 minutes. After the precipitation period, the tubes are centrifuged briefly, liquid removed, washed with 500 ml 100% ethanol and centrifuged for 30 seconds. Again the liquid is removed and 105 μl 100% ethanol is added to the final tungsten particle pellet. For particle gun bombardment, the tungsten/DNA particles are briefly sonicated and 10 μl spotted onto the center of each macrocarrier and allowed to dry about 2 minutes before bombardment.

Particle Gun Treatment:

The sample plates are bombarded at level #4 in particle gun #HE34-1 or #HE34-2. All samples receive a single shot at 650 PSI, with a total of ten aliquots taken from each tube of prepared particles/DNA.

Subsequent Treatment:

Following bombardment, the embryos are kept on 560Y medium for 2 days, then transferred to 560R selection medium containing 3 mg/liter Bialaphos and subcultured every 2 weeks. After approximately 10 weeks of selection, selection-resistant callus clones are transferred to 288J medium to initiate plant regeneration. Following somatic embryo maturation (2-4 weeks), well-developed somatic embryos are transferred to medium for germination and transferred to the lighted culture room. Approximately 7-10 days later, developing plantlets are transferred to 272V hormone-free medium in tubes for 7-10 days until plantlets are well established. Plants are then transferred to inserts in flats (equivalent to 2.5″ pot) containing potting soil and grown for 1 week in a growth chamber, subsequently grown an additional 1-2 weeks in the greenhouse, then transferred to classic 600 pots (1.6 gallon) and grown to maturity. Plants are monitored and scored for increased abiotic stress. Assays to measure improved abiotic stress are routine in the art and include, for example, increased kernel-earring capacity yields under drought conditions when compared to control maize plants under identical environmental conditions. Alternatively, the transformed plants can be monitored for a modulation in meristem development (i.e., a decrease in spikelet formation on the ear). See, for example, Bruce, et al., (2002) Journal of Experimental Botany 53:1-13.

Bombardment and Culture Media:

Bombardment medium (560Y) comprises 4.0 g/l N6 basal salts (SIGMA C-1416), 1.0 ml/l Eriksson's Vitamin Mix (1000×SIGMA-1511), 0.5 mg/l thiamine HCl, 120.0 g/l sucrose, 1.0 mg/l 2,4-D and 2.88 g/l L-proline (brought to volume with D-I H₂O following adjustment to pH 5.8 with KOH); 2.0 g/l Gelrite® (added after bringing to volume with D-I H₂O) and 8.5 mg/l silver nitrate (added after sterilizing the medium and cooling to room temperature). Selection medium (560R) comprises 4.0 g/l N6 basal salts (SIGMA C-1416), 1.0 ml/l Eriksson's Vitamin Mix (1000×SIGMA-1511), 0.5 mg/l thiamine HCl, 30.0 g/l sucrose and 2.0 mg/l 2,4-D (brought to volume with D-I H₂O following adjustment to pH 5.8 with KOH); 3.0 g/l Gelrite® (added after bringing to volume with D-I H₂O) and 0.85 mg/l silver nitrate and 3.0 mg/l bialaphos (both added after sterilizing the medium and cooling to room temperature).

Plant regeneration medium (288J) comprises 4.3 g/l MS salts (GIBCO 11117-074), 5.0 ml/l MS vitamins stock solution (0.100 g nicotinic acid, 0.02 g/l thiamine HCL, 0.10 g/l pyridoxine HCL and 0.40 g/l glycine brought to volume with polished D-I H₂O) (Murashige and Skoog, (1962) Physiol. Plant. 15:473), 100 mg/l myo-inositol, 0.5 mg/l zeatin, 60 g/l sucrose and 1.0 ml/l of 0.1 mM abscisic acid (brought to volume with polished D-I H₂O after adjusting to pH 5.6); 3.0 g/l Gelrite® (added after bringing to volume with D-I H₂O) and 1.0 mg/l indoleacetic acid and 3.0 mg/l bialaphos (added after sterilizing the medium and cooling to 60° C.). Hormone-free medium (272V) comprises 4.3 g/l MS salts (GIBCO 11117-074), 5.0 ml/l MS vitamins stock solution (0.100 g/l nicotinic acid, 0.02 g/l thiamine HCL, 0.10 g/l pyridoxine HCL and 0.40 g/l glycine brought to volume with polished D-I H₂O), 0.1 g/l myo-inositol and 40.0 g/l sucrose (brought to volume with polished D-I H₂O after adjusting pH to 5.6) and 6 g/l Bacto™-agar (added after bringing to volume with polished D-I H₂O), sterilized and cooled to 60° C.

Example 4

Agrobacterium-Mediated Transformation

For Agrobacterium-mediated transformation of maize with an antisense sequence of the nutrient uptake/stress tolerance sequence of the present disclosure, preferably the method of Zhao is employed (U.S. Pat. No. 5,981,840 and PCT Patent Publication WO 1998/32326, the contents of which are hereby incorporated by reference). Briefly, immature embryos are isolated from maize and the embryos contacted with a suspension of Agrobacterium, where the bacteria are capable of transferring the sequence to at least one cell of at least one of the immature embryos (step 1: the infection step). In this step the immature embryos are preferably immersed in an Agrobacterium suspension for the initiation of inoculation. The embryos are co-cultured for a time with the Agrobacterium (step 2: the co-cultivation step). Preferably the immature embryos are cultured on solid medium following the infection step. Following this co-cultivation period an optional “resting” step is contemplated. In this resting step, the embryos are incubated in the presence of at least one antibiotic known to inhibit the growth of Agrobacterium without the addition of a selective agent for plant transformants (step 3: resting step). Preferably the immature embryos are cultured on solid medium with antibiotic, but without a selecting agent, for elimination of Agrobacterium and for a resting phase for the infected cells. Next, inoculated embryos are cultured on medium containing a selective agent and growing transformed callus is recovered (step 4: the selection step). Preferably, the immature embryos are cultured on solid medium with a selective agent resulting in the selective growth of transformed cells. The callus is then regenerated into plants (step 5: the regeneration step) and preferably calli grown on selective medium are cultured on solid medium to regenerate the plants. Plants are monitored and scored for a modulation in meristem development, for instance, alterations of size and appearance of the shoot and floral meristems and/or increased yields of leaves, flowers and/or fruits.

Example 5
Transgenic Maize Plants Overexpressing Sorghum Genes Showed Improved Ear Traits and Yield Components

Sorghum genomic clones (SEQ ID NOS: 3553, 3563, 3564, 3589, 3680, 4042, 4548, 4202, 4306, 4345, 4530, 4724, 4887, 4910) containing the corresponding 13 genes were isolated and each individual gene was transformed into maize plants. In the designed vector, transgene expression was driven by a constitutive maize ubiquitin promoter. TO plants overexpressing the transgenes were generated. Transgenic plants from multiple events were subjected to T1 reproductive assay under low nitrogen stress treatment (4 mM concentration). Multiple ear traits were collected from multiple events of the transgenic plants corresponding to these 13 genes, respectively. Compared to non-transgenic controls, the transgenic plants showed significant improvement in plant growth especially ear traits, such as ear length, ear width, ear area and silk number, which reflects the seed number potential per ear (Table 2, below). These data demonstrate the efficacy of these sorghum genes in improving yield components and potential yield of maize and under stressed condition of low nitrogen.

TABLE 2

Seq

ID

Ear length
Ear width
Silk count
Stress

Gene Loc
NO
Ear area (cm²)
(cm)
(cm)
(no.)
type

Sb03g011680
3553
2/6 events sig
2/6 events sig
2/6 events
2/6 events
NUE

increase up to
increase up to
sig increase
sig increase
and

31%
22%
up to 8%
up to 23%
Drought

Sb06g033870
3563
1/6 events sig
1/6 events sig
4/6 events;
1/6 events
NUE

increase up to
increase up to
NS
sig increase
and

24%
18%

up to 16%
Drought

Sb03g034260
3564
1/6 events sig
3/6 events; NS
3/6 events;
6/6 events;
NUE

increase up to

NS
NS

15%

Sb09g029110
3589
2/6 events sig
2/6 events sig
2/6 events
2/6 events
NUE

increase up to
increase up to
sig increase
sig increase

32%
18%
up to 11%
up to 32%

Sb03g029150
3680
1/6 events sig
4/6 events; NS
1/6 events
3/6 events;
NUE

increase up to

sig increase
NS

16%

up to 5%

Sb02g000230
4042
4/5 events; NS
4/5 events; NS
4/5 events;
4/5 events;
NUE

NS
NS

Sb04g034130
4548
1/6 events sig
5/6 events; NS
1/6 events
1/6 events
NUE

increase up to

sig increase
sig increase

18%

up to 6%
up to 18%

Sb02g041830
4202
2/3 events; NS
2/3 events; NS
2/3 events;
2/3 events
NUE

NS
sig increase

up to 26%

Sb03g027470
4306
6/6 events; NS
1/6 events sig
1/6 events
1/6 events
NUE

increase up
sig increase
sig increase

to 14%
up to 6%
up to 16%

Sb03g034500
4345
2/3 events; NS
2/3 events; NS
2/3 events;
3/3 events;
NUE

NS
NS

Sb04g030895
4530
1/6 events sig
1/6 events sig
1/6 events
3/6 events;
NUE

increase up to
increase up to
sig increase
NS

21%
16%
up to 6%

Sb06g029070
4724
1/6 events sig
2/6 events sig
1/6 events
1/6 events;
NUE

increase up to
increase up to
sig increase
NS

31%
26%
up to 10%

Sb08g021630
4887
4/6 events; NS
3/6 events; NS
3/6 events;
3/6 events;
NUE

NS
NS

Sb09g004150
4910
4/6 events; NS
4/6 events; NS
2/6 events;
1/6 events
Drought

NS
sig increase

up to 25%

NS - increase not significant

P < 0.10

Example 6
Soybean Embryo Transformation

Soybean embryos are bombarded with a plasmid containing nutrient uptake/stress tolerance sequence operably linked to an ubiquitin promoter as follows. To induce somatic embryos, cotyledons, 3-5 mm in length dissected from surface-sterilized, immature seeds of the soybean cultivar A2872, are cultured in the light or dark at 26° C. on an appropriate agar medium for six to ten weeks. Somatic embryos producing secondary embryos are then excised and placed into a suitable liquid medium. After repeated selection for clusters of somatic embryos that multiplied as early, globular-staged embryos, the suspensions are maintained as described below.

Soybean embryogenic suspension cultures can be maintained in 35 ml liquid media on a rotary shaker, 150 rpm, at 26° C. with florescent lights on a 16:8 hour day/night schedule. Cultures are subcultured every two weeks by inoculating approximately 35 mg of tissue into 35 ml of liquid medium.

Soybean embryogenic suspension cultures may then be transformed by the method of particle gun bombardment (Klein, et al., (1987) Nature (London) 327:70-73, U.S. Pat. No. 4,945,050). A Du Pont Biolistic PDS1000/HE instrument (helium retrofit) can be used for these transformations.

A selectable marker gene that can be used to facilitate soybean transformation is a transgene composed of the 35S promoter from Cauliflower Mosaic Virus (Odell, et al., (1985) Nature 313:810-812), the hygromycin phosphotransferase gene from plasmid pJR225 (from E. coli; Gritz, et al., (1983) Gene 25:179-188) and the 3′ region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens. The expression cassette comprising nutrient uptake/stress tolerance sense sequence operably linked to the ubiquitin promoter can be isolated as a restriction fragment. This fragment can then be inserted into a unique restriction site of the vector carrying the marker gene.

To 50 μl of a 60 mg/ml 1 μm gold particle suspension is added (in order): 5 μl DNA (1 μg/μl), 20 μl spermidine (0.1 M), and 50 μl CaCl₂(2.5 M). The particle preparation is then agitated for three minutes, spun in a microfuge for 10 seconds and the supernatant removed. The DNA-coated particles are then washed once in 400 μl 70% ethanol and resuspended in 40 μl of anhydrous ethanol. The DNA/particle suspension can be sonicated three times for one second each. Five microliters of the DNA-coated gold particles are then loaded on each macro carrier disk.

Approximately 300-400 mg of a two-week-old suspension culture is placed in an empty 60×15 mm petri dish and the residual liquid removed from the tissue with a pipette. For each transformation experiment, approximately 5-10 plates of tissue are normally bombarded. Membrane rupture pressure is set at 1100 psi, and the chamber is evacuated to a vacuum of 28 inches mercury. The tissue is placed approximately 3.5 inches away from the retaining screen and bombarded three times. Following bombardment, the tissue can be divided in half and placed back into liquid and cultured as described above.

Five to seven days post bombardment, the liquid media may be exchanged with fresh media, and eleven to twelve days post-bombardment with fresh media containing 50 mg/ml hygromycin. This selective media can be refreshed weekly. Seven to eight weeks post-bombardment, green, transformed tissue may be observed growing from untransformed, necrotic embryogenic clusters. Isolated green tissue is removed and inoculated into individual flasks to generate new, clonally propagated, transformed embryogenic suspension cultures. Each new line may be treated as an independent transformation event. These suspensions can then be subcultured and maintained as clusters of immature embryos or regenerated into whole plants by maturation and germination of individual somatic embryos.

Example 7
Sunflower Meristem Tissue Transformation

Sunflower meristem tissues are transformed with an expression cassette containing the nutrient uptake/stress tolerance sequence operably linked to a ubiquitin promoter as follows (see also, EP Patent Number 0 486233, herein incorporated by reference and Malone-Schoneberg, et al., (1994) Plant Science 103:199-207). Mature sunflower seed (Helianthus annuus L.) are dehulled using a single wheat-head thresher. Seeds are surface sterilized for 30 minutes in a 20% Clorox® bleach solution with the addition of two drops of Tween® 20 per 50 ml of solution. The seeds are rinsed twice with sterile distilled water.

Split embryonic axis explants are prepared by a modification of procedures described by Schrammeijer, et al., (Schrammeijer, et al., (1990) Plant Cell Rep. 9:55-60). Seeds are imbibed in distilled water for 60 minutes following the surface sterilization procedure. The cotyledons of each seed are then broken off, producing a clean fracture at the plane of the embryonic axis. Following excision of the root tip, the explants are bisected longitudinally between the primordial leaves. The two halves are placed, cut surface up, on GBA medium consisting of Murashige and Skoog mineral elements (Murashige, et al., (1962) Physiol. Plant., 15:473-497), Shepard's vitamin additions (Shepard, (1980) in Emergent Techniques for the Genetic Improvement of Crops (University of Minnesota Press, St. Paul, Minn.), 40 mg/l adenine sulfate, 30 g/l sucrose, 0.5 mg/l 6-benzyl-aminopurine (BAP), 0.25 mg/I indole-3-acetic acid (IAA), 0.1 mg/l gibberellic acid (GA₃), pH 5.6 and 8 g/l Phytagar.

The explants are subjected to microprojectile bombardment prior to Agrobacterium treatment (Bidney, et al., (1992) Plant Mol. Biol. 18:301-313). Thirty to forty explants are placed in a circle at the center of a 60×20 mm plate for this treatment. Approximately 4.7 mg of 1.8 mm tungsten microprojectiles are resuspended in 25 ml of sterile TE buffer (10 mM Tris HCl, 1 mM EDTA, pH 8.0) and 1.5 ml aliquots are used per bombardment. Each plate is bombarded twice through a 150 mm nytex screen placed 2 cm above the samples in a PDS 1000® particle acceleration device.

Disarmed Agrobacterium tumefaciens strain EHA105 is used in all transformation experiments. A binary plasmid vector comprising the expression cassette that contains the nutrient uptake/stress tolerance gene operably linked to the ubiquitin promoter is introduced into Agrobacterium strain EHA105 via freeze-thawing as described by Holsters, et al., (1978) Mol. Gen. Genet. 163:181-187. This plasmid further comprises a kanamycin selectable marker gene (i.e, nptII). Bacteria for plant transformation experiments are grown overnight (28° C. and 100 RPM continuous agitation) in liquid YEP medium (10 gm/l yeast extract, 10 gm/l Bacto® peptone and 5 gm/l NaCl, pH 7.0) with the appropriate antibiotics required for bacterial strain and binary plasmid maintenance. The suspension is used when it reaches an OD₆₀₀of about 0.4 to 0.8. The Agrobacterium cells are pelleted and resuspended at a final OD₆₀₀of 0.5 in an inoculation medium comprised of 12.5 mM MES pH 5.7, 1 gm/l NH₄Cl and 0.3 gm/l MgSO₄.

Freshly bombarded explants are placed in an Agrobacterium suspension, mixed, and left undisturbed for 30 minutes. The explants are then transferred to GBA medium and co-cultivated, cut surface down, at 26° C. and 18-hour days. After three days of co-cultivation, the explants are transferred to 374B (GBA medium lacking growth regulators and a reduced sucrose level of 1%) supplemented with 250 mg/l cefotaxime and 50 mg/l kanamycin sulfate. The explants are cultured for two to five weeks on selection and then transferred to fresh 374B medium lacking kanamycin for one to two weeks of continued development. Explants with differentiating, antibiotic-resistant areas of growth that have not produced shoots suitable for excision are transferred to GBA medium containing 250 mg/l cefotaxime for a second 3-day phytohormone treatment. Leaf samples from green, kanamycin-resistant shoots are assayed for the presence of NPTII by ELISA and for the presence of transgene expression by assaying for a modulation in meristem development (i.e., an alteration of size and appearance of shoot and floral meristems).

NPTII-positive shoots are grafted to Pioneer® hybrid 6440 in vitro-grown sunflower seedling rootstock. Surface sterilized seeds are germinated in 48-0 medium (half-strength Murashige and Skoog salts, 0.5% sucrose, 0.3% Gelrite®, pH 5.6) and grown under conditions described for explant culture. The upper portion of the seedling is removed, a 1 cm vertical slice is made in the hypocotyl, and the transformed shoot inserted into the cut. The entire area is wrapped with Parafilm® to secure the shoot. Grafted plants can be transferred to soil following one week of in vitro culture. Grafts in soil are maintained under high humidity conditions followed by a slow acclimatization to the greenhouse environment. Transformed sectors of T₀plants (parental generation) maturing in the greenhouse are identified by NPTII ELISA and/or by nutrient uptake/stress tolerance activity analysis of leaf extracts while transgenic seeds harvested from NPTII-positive T₀plants are identified by nutrient uptake/stress tolerance activity analysis of small portions of dry seed cotyledon.

An alternative sunflower transformation protocol allows the recovery of transgenic progeny without the use of chemical selection pressure. Seeds are dehulled and surface-sterilized for 20 minutes in a 20% Clorox® bleach solution with the addition of two to three drops of Tween® 20 per 100 ml of solution, then rinsed three times with distilled water. Sterilized seeds are imbibed in the dark at 26° C. for 20 hours on filter paper moistened with water. The cotyledons and root radical are removed, and the meristem explants are cultured on 374E (GBA medium consisting of MS salts, Shepard vitamins, 40 mg/l adenine sulfate, 3% sucrose, 0.5 mg/l 6-BAP, 0.25 mg/l IAA, 0.1 mg/l GA, and 0.8% Phytagar at pH 5.6) for 24 hours under the dark. The primary leaves are removed to expose the apical meristem, around 40 explants are placed with the apical dome facing upward in a 2 cm circle in the center of 374M (GBA medium with 1.2% Phytagar) and then cultured on the medium for 24 hours in the dark.

Approximately 18.8 mg of 1.8 μm tungsten particles are resuspended in 150 μl absolute ethanol. After sonication, 8 μl of it is dropped on the center of the surface of macrocarrier. Each plate is bombarded twice with 650 psi rupture discs in the first shelf at 26 mm of Hg helium gun vacuum.

The plasmid of interest is introduced into Agrobacterium tumefaciens strain EHA105 via freeze thawing as described previously. The pellet of overnight-grown bacteria at 28° C. in a liquid YEP medium (10 g/l yeast extract, 10 g/l Bacto® peptone and 5 g/l NaCl, pH 7.0) in the presence of 50 μg/l kanamycin is resuspended in an inoculation medium (12.5 mM 2-mM 2-(N-morpholino) ethanesulfonic acid, MES, 1 g/l NH₄CI and 0.3 g/l MgSO₄at pH 5.7) to reach a final concentration of 4.0 at OD 600. Particle-bombarded explants are transferred to GBA medium (374E) and a droplet of bacteria suspension is placed directly onto the top of the meristem. The explants are co-cultivated on the medium for 4 days, after which the explants are transferred to 374C medium (GBA with 1% sucrose and no BAP, IAA, GA3 and supplemented with 250 μg/ml cefotaxime). The plantlets are cultured on the medium for about two weeks under 16-hour day and 26° C. incubation conditions.

Explants (around 2 cm long) from two weeks of culture in 374C medium are screened for a modulation in meristem development (i.e., an alteration of size and appearance of shoot and floral meristems). After positive (i.e., a change in nutrient uptake/stress tolerance expression) explants are identified, those shoots that fail to exhibit an alteration in nutrient uptake/stress tolerance activity are discarded and every positive explant is subdivided into nodal explants. One nodal explant contains at least one potential node. The nodal segments are cultured on GBA medium for three to four days to promote the formation of auxiliary buds from each node. Then they are transferred to 374C medium and allowed to develop for an additional four weeks. Developing buds are separated and cultured for an additional four weeks on 374C medium. Pooled leaf samples from each newly recovered shoot are screened again by the appropriate protein activity assay. At this time, the positive shoots recovered from a single node will generally have been enriched in the transgenic sector detected in the initial assay prior to nodal culture.

Recovered shoots positive for altered nutrient uptake/stress tolerance expression are grafted to Pioneer hybrid 6440 in vitro-grown sunflower seedling rootstock. The rootstocks are prepared in the following manner. Seeds are dehulled and surface-sterilized for 20 minutes in a 20% Clorox® bleach solution with the addition of two to three drops of Tween® 20 per 100 ml of solution, and are rinsed three times with distilled water. The sterilized seeds are germinated on the filter moistened with water for three days, then they are transferred into 48 medium (half-strength MS salt, 0.5% sucrose, 0.3% Gelrite® pH 5.0) and grown at 26° C. under the dark for three days, then incubated at 16-hour-day culture conditions. The upper portion of selected seedling is removed, a vertical slice is made in each hypocotyl, and a transformed shoot is inserted into a V-cut. The cut area is wrapped with Parafilm®. After one week of culture on the medium, grafted plants are transferred to soil. In the first two weeks, they are maintained under high humidity conditions to acclimatize to a greenhouse environment.

Example 8
Abiotic Stress Screening of Transgenic Plants Expressing Sorghum Stress Tolerance Proteins

A qualitative drought screen was performed with plants over-expressing different sorghum stress tolerance genes under the control of different promoters. The soil is watered to saturation and then plants are grown under standard conditions (i.e., 16 hour light, 8 hour dark cycle; 22° C.; ˜60% relative humidity). No additional water is given.

Digital images of the plants are taken at the onset of visible drought stress symptoms. Images are taken once a day (at the same time of day), until the plants appear dessicated. Typically, four consecutive days of data is captured.

Color analysis is employed for identifying potential drought tolerant lines. Color analysis can be used to measure the increase in the percentage of leaf area that falls into a yellow color bin. Using hue, saturation and intensity data (“HSI”), the yellow color bin consists of hues 35 to 45.

Maintenance of leaf area is also used as another criterion for identifying potential drought tolerant lines, since Arabidopsis leaves wilt during drought stress. Maintenance of leaf area can be measured as reduction of rosette leaf area over time. Leaf area is measured in terms of the number of green pixels obtained using the LemnaTec imaging system. Transgenic and non-transgenic control plants are grown side by side in flats.

When wilting begins, images are taken for a number of days to monitor the wilting process. From these data wilting profiles are determined based on the green pixel counts obtained over four consecutive days for transgenic and accompanying control plants. The profile is selected from a series of measurements over the four day period that gives the largest degree of wilting.

The ability to withstand drought is measured by the tendency of transgenic plants to resist wilting compared to control plants.

Estimates of the leaf area of the Arabidopsis plants are obtained in terms of the number of green pixels. The data for each image is averaged to obtain estimates of mean and standard deviation for the green pixel counts for transgenic and non-transgenic control plants. Parameters for a noise function are obtained by straight line regression of the squared deviation versus the mean pixel count using data for all images in a batch. Error estimates for the mean pixel count data are calculated using the fit parameters for the noise function. The mean pixel counts for transgenic and control plants are summed to obtain an assessment of the overall leaf area for each image. The four-day interval with maximal wilting is obtained by selecting the interval that corresponds to the maximum difference in plant growth. The individual wilting responses of the transgenic and control plants are obtained by normalization of the data using the value of the green pixel count of the first day in the interval. The drought tolerance of the transgenic plant compared to the control plant is scored by summing the weighted difference between the wilting response of transgenic plants and control plants over day two to day four; the weights are estimated by propagating the error in the data. A positive drought tolerance score corresponds to a transgenic plant with slower wilting compared to the control plant. Significance of the difference in wilting response between transgenic and control plants is obtained from the weighted sum of the squared deviations.

Transgenic events with a significant delay in yellow color accumulation and/or with significant maintenance of rosette leaf area, when compared to the control are considered drought tolerant

Example 9
Variants of Sequences

A. Variant Nucleotide Sequences that do not Alter the Encoded Amino Acid Sequence

The nucleotide sequences are used to generate variant nucleotide sequences having the nucleotide sequence of the open reading frame with about 70%, 75%, 80%, 85%, 90% and 95% nucleotide sequence identity when compared to the starting unaltered ORF nucleotide sequence of the corresponding SEQ ID NO. These functional variants are generated using a standard codon table. While the nucleotide sequences of the variants are altered, the amino acid sequence encoded by the open reading frames does not change.

B. Variant Amino Acid Sequences of Polypeptides

Variant amino acid sequences of the polypeptides are generated. In this example, one amino acid is altered. Specifically, the open reading frames are reviewed to determine the appropriate amino acid alteration. The selection of the amino acid to change is made by consulting the protein alignment (with the other orthologs and other gene family members from various species). An amino acid is selected that is deemed not to be under high selection pressure (not highly conserved) and which is rather easily substituted by an amino acid with similar chemical characteristics (i.e., similar functional side-chain). Using a protein alignment, an appropriate amino acid can be changed. Once the targeted amino acid is identified, the procedure outlined in the following section C is followed. Variants having about 70%, 75%, 80%, 85%, 90% and 95% nucleic acid sequence identity are generated using this method.

C. Additional Variant Amino Acid Sequences of Polypeptides

In this example, artificial protein sequences are created having 80%, 85%, 90% and 95% identity relative to the reference protein sequence. This latter effort requires identifying conserved and variable regions and then the judicious application of an amino acid substitutions table. These parts will be discussed in more detail below.

Largely, the determination of which amino acid sequences are altered is made based on the conserved regions among protein or among the other polypeptides. Based on the sequence alignment, the various regions of the polypeptide that can likely be altered are represented in lower case letters, while the conserved regions are represented by capital letters. It is recognized that conservative substitutions can be made in the conserved regions below without altering function. In addition, one of skill will understand that functional variants of the sequence of the disclosure can have minor non-conserved amino acid alterations in the conserved domain.

Artificial protein sequences are then created that are different from the original in the intervals of 80-85%, 85-90%, 90-95% and 95-100% identity. Midpoints of these intervals are targeted, with liberal latitude of plus or minus 1%, for example. The amino acids substitutions will be effected by a custom Perl script. The substitution table is provided below in Table 3.

TABLE 3

Substitution Table

Strongly

Similar and
Rank of

Optimal
Order to

Amino Acid
Substitution
Change
Comment

I
L, V
1
50:50 substitution

L
I, V
2
50:50 substitution

V
I, L
3
50:50 substitution

A
G
4

G
A
5

D
E
6

E
D
7

W
Y
8

Y
W
9

S
T
10

T
S
11

K
R
12

R
K
13

N
Q
14

Q
N
15

F
Y
16

M
L
17
First methionine cannot change

H

Na
No good substitutes

C

Na
No good substitutes

P

Na
No good substitutes

First, any conserved amino acids in the protein that should not be changed is identified and “marked off” for insulation from the substitution. The start methionine will of course be added to this list automatically. Next, the changes are made.

H, C and P are not changed in any circumstance. The changes will occur with isoleucine first, sweeping N-terminal to C-terminal. Then leucine and so on down the list until the desired target it reached. Interim number substitutions can be made so as not to cause reversal of changes. The list is ordered 1-17, so start with as many isoleucine changes as needed before leucine and so on down to methionine. Clearly many amino acids will in this manner not need to be changed. L, I and V will involve a 50:50 substitution of the two alternate optimal substitutions.

The variant amino acid sequences are written as output. Perl script is used to calculate the percent identities. Using this procedure, variants of the polypeptides are generating having about 80%, 85%, 90% and 95% amino acid identity to the disclosed sequences.

All publications and patent applications in this specification are indicative of the level of ordinary skill in the art to which this disclosure pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated by reference.

The disclosure has been described with reference to various specific and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the disclosure.

GENES FOR IMPROVING NUTRIENT UPTAKE AND ABIOTIC STRESS TOLERANCE IN PLANTS

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