Claims
- 1. A recombinant ZCCT1 protein coding sequence comprising a nucleic acid that hybridizes to a nucleic acid molecule encoding SEQ ID NO:75 under hybridization conditions that include at least one wash in 0.1×SSC and 0.1% SDS at 60-65° for thirty minutes.
- 2. The recombinant ZCCT1 protein coding sequence of claim 1 wherein said sequence is operably linked to a heterologous promoter.
- 3. The recombinant ZCCT1 protein coding sequence of claim 3 wherein the heterologous promoter is an inducible promoter.
- 4. The recombinant ZCCT1 protein coding sequence of claim 3 wherein the heterologous promoter is a regulated promoter.
- 5. The recombinant ZCCT1 protein coding sequence of claim 3 wherein the heterologous promoter is a constitutive promoter.
- 6. A vector comprising the recombinant ZCCT1 protein coding sequence of claim 1.
- 7. A vector comprising the recombinant ZCCT1 protein coding sequence of claim 2.
- 8. A vector comprising the recombinant ZCCT1 protein coding sequence of claim 3.
- 9. A vector comprising the recombinant ZCCT1 protein coding sequence of claim 4.
- 10. A vector comprising the recombinant ZCCT1 protein coding sequence of claim 5.
- 11. A cell comprising the vector of claim 6.
- 12. A cell comprising the vector of claim 8.
- 13. A cell comprising the vector of claim 9.
- 14. The cell of claim 11 wherein said cell is a plant cell.
- 15. The cell of claim 12 wherein said cell is a plant cell.
- 16. The cell of claim 13 wherein said cell is a plant cell.
- 17. A transgenic plant comprising the recombinant ZCCT1 protein coding sequence of claim 1.
- 18. The transgenic plant of claim 17 wherein said plant is selected from the group consisting of wheat, barley, rye, oats, and forage grasses.
- 19. Seed from the transgenic plant of claim 17.
- 20. A transgenic plant comprising the recombinant ZCCT1 protein coding sequence of claim 3.
- 21. Seed from the transgenic plant of claim 20.
- 22. A transgenic plant comprising the recombinant ZCCT1 protein coding sequence of claim 4.
- 23. Seed from the transgenic plant of claim 22.
- 24. A method for altering a plant's response to vernalization, the method comprising: transforming a plant or plant tissue with a genetic construct comprising the recombinant ZCCT1 protein coding sequence as in claim 3 and inducing the expression of the genetic construct in said plant to alter said plant's response to vernalization.
- 25. A method for altering a plant's response to vernalization, the method comprising: transforming a plant or plant tissue with a genetic construct comprising the recombinant ZCCT1 protein coding sequence as in claim 4 and expressing the genetic construct in said plant to alter said plant's response to vernalization.
- 26. The method of claim 24, wherein the plant is selected from the group consisting wheat, barley, rye, oats, and forage grasses.
- 27. The method of claim 25, wherein the plant is selected from the group consisting wheat, barley, rye, oats, and forage grasses.
- 28. A transgenic plant comprising a genetic construct that inhibits ZCCT1 repression of AP1.
- 29. The transgenic plant of claim 28 wherein the genetic construct is an RNAi construct that inhibits ZCCT1 activity.
- 30. The transgenic plant of claim 28 wherein the genetic construct comprises an antisense construct that inhibits ZCCT1 activity.
- 31. The transgenic plant of claim 28 wherein the genetic construct comprises a nucleic acid sequence encoding a repression defective ZCCT1 protein operably linked to a promoter.
- 32. The transgenic plant of claim 28 wherein the genetic construct comprises a nucleic acid sequence encoding a DNA binding defective ZCCT1 protein operably linked to a promoter.
- 33. A molecular marker for Vrn2 derived from SEQ ID NO:75.
RELATED APPLICATIONS
[0001] This application is a continuation-in-part application of U.S. application Ser. No. 10/412,137, filed Apr. 11, 2003, which is incorporated by reference herein in its entirety.
GOVERNMENT INTEREST
[0002] This invention was made with Government support under Grant (or Contract) No. 2000-1678 awarded by the USDA-NRI. The Government has certain rights in the invention.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
10412137 |
Apr 2003 |
US |
Child |
10723947 |
Nov 2003 |
US |