Claims
- 1. A method of purifying a recombinant heparanase from overexpressing cells or growth medium comprising adsorbing said recombinant heparanase on an ion-exchange column under low salt conditions, washing said column with low salt solution thereby eluting other proteins, and eluting the recombinant heparanase from said column by a salt gradient or higher salt concentration.
- 2. The method of claim 1, wherein said ion-exchange column is a Source-S column.
- 3. The method of claim 1, wherein said recombinant heparanase includes an amino acid sequence at least 70% homologous to SEQ ID NO:2.
- 4. The method of claim 1, wherein said recombinant heparanase includes an amino acid sequence as set forth in SEQ ID NO:2.
- 5. A method of affinity purifying heparanase comprising:
(a) contacting a heparanase preparation with an affinity substrate including a solid matrix and an anti-heparanase antibody being immobilized thereto, said anti-heparanase antibody being capable of specifically binding at least one epitope of said heparanase; (b) washing said affinity substrate; and (c) eluting heparanase molecules being adsorbed on said affinity substrate via said anti-heparanase antibody.
- 6. The method of claim 5, wherein said heparanase includes an amino acid sequence at least 70% homologous to SEQ ID NO:2.
- 7. The method of claim 5, wherein said heparanase includes an amino acid sequence as set forth in SEQ ID NO:2.
Parent Case Info
[0001] This is a continuation of U.S. patent application Ser. No. 09/635,923, filed Aug. 10, 2000, which is a continuation of U.S. patent application Ser. No. 09/487,716, filed Jan. 19, 2000, which is a continuation of U.S. patent application Ser. No. 09/260,038, filed Mar. 2, 1999, now U.S. Pat. No. 6,348,344, issued Feb. 19, 2002, which is a continuation-in-part of U.S. patent application Ser. No. 09/071,618, filed May 1, 1998, now abandoned, which is a continuation-in-part of U.S. patent application Ser. No. 09/071,739, filed May 1, 1998, now U.S. Pat. No. 6,177,545, issued Jan. 23, 2002, which is a continuation-in-part of U.S. patent application Ser. No. 08/922,170, filed Sep. 2, 1997, now U.S. Pat. No. 5,968,822, issued Oct. 19, 1999.
Continuations (3)
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09635923 |
Aug 2000 |
US |
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10137351 |
May 2002 |
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09487716 |
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09635923 |
Aug 2000 |
US |
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09260038 |
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09487716 |
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Continuation in Parts (3)
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09071618 |
May 1998 |
US |
Child |
09260038 |
Mar 1999 |
US |
Parent |
09071739 |
May 1998 |
US |
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09071618 |
May 1998 |
US |
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08922170 |
Sep 1997 |
US |
Child |
09071739 |
May 1998 |
US |