Glucan chain length domains

Abstract

The invention relates to a method for changing the glucan chain lengths using fusion protein domains of various starch synthase enzymes in any starch or starch granule producing organism. The invention relates to identification of a GLucan ASSociation domain (herein after referred to as “GLASS” domain) of granule bound starch synthase (GBSS) used in combination with any other GLYcosyl TRransferase domain otherwise referred to as pfam00534-catalytic domain (herein after referred to as “GLYTR” domain) of one or more of any of the other starch synthase enzymes. The invention relates to identifying and using the new and surprising discovery that starch synthases are composed of at least two distinct functional domains herein after labeled as “GLASS” and “GLYTR”. More specifically, this invention relates to the genetic constructs that encode the fusions of the above domains and to the plants transformed with said constructs. The method of invention can thus be used in particular to provide a modified profile of starch granule associated starch synthase (SS) enzymes and by which modified glucan chain lengths of amylopectin and hence, modified starches and or complexes will be generated. This can be done in any organism and more particularly any plant that stores or synthesizes starch in any of its parts, such as potato, sweet potato, cassaya, pea, taro, banana, yam and cereal crops such as rice, maize, wheat, barley, oats, and sorghum.

Description

[0002] The development of genetic engineering techniques has made it possible to transfer genes from various organisms and plants into other organisms or plants. Although starch has been altered by transformation and mutagenesis in the past, there is still a great need for further starch M modifications in order to meet the ever-increasing industrial need for variations of this natural polymer. Toward this end, there is a need to manipulate the chain lengths of glucan chains in amylopectin in order to provide novel starches with multitude of industrial uses. In order to achieve this goal the present invention is directed at introducing changes in the ratios, composition, and functionality of various enzymes in the starch synthesis pathway.

[0003] The present invention relates to novel plants expressing transgenic genes and having an altered ability to produce specialty starch traits with modified glucan chain lengths. Modification of starch biosynthesis pathways by changing the functionality of starch synthase enzymes has an enormous potential for production of new and improved starches. Maize starch is used to produce a wide range of food products (for human and animal consumption) and several industrial products. Several crop varieties are known which produce different types of starch. The type or quality of starch makes it suitable for certain purposes, including particular methods of processing or particular end-uses. For example, U.S. Pat. Nos. 4,789,557, 4,790,997, 4,774,328, 4,770,710, 4,798,735, 4,767,849, 4,801,470, 4,789,738, 4,792,458 and 5,009,911 describe naturally occurring maize mutants producing starches of differing fine structure suitable for use in various food products. Although known mutants produce altered starch, some of these lines are not suitable for crop breeding and/or for the farmers' purposes. By combining various mutants together, such as double, triple and quadruple mutants it is possible to create a variety of plant starches. One key element that is lacking in these starches is the ability to control glucan chain length in the amylopectin molecule. Moreover, such mutant plants often give relatively poor yields and also low gemination rates.

[0004] Glucan chain length and chain length distribution are the two key components that determine the functionality of any given starch. Glucan chain lengths can be modified by genetic manipulation of enzymes known to possess other favorable characteristics. For example, by manipulating the function and expression levels of one or more starch synthesizing enzyme genes in a plant, it is possible to significantly alter the type of starch produced. The present invention has led to a new undertstanding of how different starch synthase (SS) enzymes recognize and have specific catalytic capabilities to various lengths of glucan chains (See also, Commuri and Keeling, 2001, The Plant Journal, 25(5), 475-486; and Commuri et al., 2002, in review, The Plant Journal). Comparative analysis have recently been completed on the functionality and catalytic properties of various maize starch synthase enzymes, namely, SSI, SSIIa, SSIIb, granule bound starch synthase (GBSS), and Du1 (SSIII) using glucan substrates with varying average chain lengths (FIGS. 1, 2 &3). Each one of these enzymes possessed different glucan chain length specificities. In maize endosperm, not all the SS enzymes are expressed at the same levels nor localized uniformly in the amyloplast stroma, and/or starch granules. Thus, each form of SS enzyme must contribute in a unique and a specific way in setting the starch structure, and there exists an enormous potential to bring a modification to the structure of starch by alteration of their location of expression and manipulating the levels of activity of these enzymes in the endosperm. Of the five different SS enzymes known to date in maize, GBSS enzyme has the highest affinity to amylopectin followed by SSI (Table 1). It is because of this affinity for its glucan substrate that most of the protein entrapped in the starch granules is comprised of GBSS (˜60%) and SSI enzymes (FIG. 4). Enzymes like SSIIa or SSIIb are undetectable in the granule and are present in low amounts in the amyloplast stroma. The Du1 protein is barely detectable in the granules and is found in reasonable amounts in the amyloplast stroma. Different forms of starch synthases are broadly conserved in evolution and it is reasonable to propose that specific functions have been selected for each one of these isoforms (Myers et al., 2000). For example, in the dull1 (Wang et al., 1993) and sugary 2 (Takeda and Preiss, 1993) mutants that affect enzymes other than SSI (Gao et al., 1998, Harn et al., 1998), both mutations decrease the proportion of the intermediate and average length of long chains (B2 and B3) in the B amylopectin relative to the short (A and B1) chains. This suggests that the enzymes other than SSI are relatively less needed for synthesis of short chains, but are needed for the synthesis of intermediate and longer chains. Furthermore, dull 1 mutation in maize eliminates SS III and the amylopectin from this mutant endosperm is enriched in short chains. In maize, potato and pea, genes for all three forms of SS exist, however, the dominant activity in maize endosperm is SSI, whereas in pea embryos it is SSII, and in potato tubers it is SSIII. It is most interesting to discover in applicants' recent studies using maize that the different SS enzymes have different catalytic capabilities to synthesize various glucan chain lengths. For example, SSI synthesizes shorter chains whereas

TABLE 1
A Comparison of Kd values of Maize Granule
Bound Starch Synthase (GBSS) and SS1-2
	Temperature (° C.) 4	Room Temperature (˜23.5)
Type of	GBSS	SSI-2	GBSS	SSI-2
Glucan	mM
Amylose	0.14 ± 0.01	0.35 ±	0.150 ± 0.01	1.06 ± 0.12
		0.07a
Amylo-	0.015 ± 0.001	0.06 ± 0.00	0.054 ± 0.004	0.07 ± 0.03
pectin
Glycogen	1.50 ± 0.369	1.20 ± 0.03	(no binding)	3.38 ± 0.83
a= The molar concentration is based on the average outer chain length (OCL) of the substrate molecule (For amylose, amylopectin, and glycogen the apparent average chain lengths are 8-9, 11-12, and 6-7, respectively).

[0005]

TABLE II
Comparison of K-values of maize SSI and SSI-2,
with α-amylase (porcine pancreas) and
glucoamylase (Aspergillus niger)
	Maize SSI	Maize SSI-2	α-amylase	glucoamylase
Substrate	mg/mL	mM	mg/mL	mM	mg/mL	mM	mg/mL	mM
Starch	0.261	0.069a	0.241	0.065a	0.539	0.144a	0.090	0.024a
Amylopectin	0.217	0.076	0.242	0.078	0.602	0.212	0.030	0.010
a = Molar concentration is calculated based on the average chain length (C.L.) of the substrate molecule.

[0006] GBSS synthesizes very long chains. SSIIa and SSIIb synthesize shorter and more intermediate chains, and SSIII (DuI) synthesizes relatively long chains. Also, these enzymes differ in their glucan binding affinities. SSIIa does not bind to any given glucan of any particular chain lengths that we tested, where as SSIIb displayed partial or minor glucan affinity and SSI binds with greatest affinity to longer glucan chains in amylopectin. This observation explains why SSIIa or SSIIb enzymes are not entrapped in the starch granules and SSI does entrap during the course of starch synthesis. The present invention provides modified starch, and methods of making and using the same, by, for example, structural modification by genetic manipulation of SS enzymes, which is possible due to the presently disclosed discovery of the specificity displayed by the enzymes described herein to a given glucan chain length. This can be accomplished by several ways and listed below and described herein are a few examples: (a) regulating the expression levels of SS enzymes, (b) alteration of the starch biosynthetic pathway by incorporation of the genes encoding one or more enzymes involved in the starch or glycogen biosynthetic pathway, (c) increasing the association of SSIIa, SSIIb and Du1 with the starch granules especially, by engineering entrapment of their corresponding enzymes with the starch granules, and (d) entrapment of fusion proteins of SS enzymes, for example, catalytic domains of SSIIa, SSIIb and Du1 in association with glucan binding domains of GBSS or SSI in the starch granules to bring a change in the glucan chain lengths and distribution and thereby synthesize modified starch.

[0007] U.S. Pat. Nos. 5,824,790, 6,130,367, and 5,300,145 describe methods of (a) and (b) of the above. The international application WO 92/11376 describes a method for suppressing amylose formation in potato by transforming potato plant with a construct comprising antisense fragments designed to inhibit the expression of GBSS gene. The Canadian patent application 2,061,143 describes a similar technique for producing amylose free potato starch. The production of modified starches by plants transformed with genes encoding enzymes involved in starch synthesis is described for example in DE-A-19534759, WO 92/14827 in which branching enzyme derived from potato cDNA is used and WO 92/11376 describes an alternative method for antisense suppression of GBSS activity in plants.

[0008] However, none of these above mentioned patents describe the combination of different domains of SS enzymes, for example starch association domain (“GLASS” domain) of one enzyme, like GBSS to the catalytic domain (“GLYTR” domain) of another, for example SSIIa, in order to bring a modification to the structure of starch. Surprisingly, the applicants discovery of threading SS enzymes using 3D-PSSM (three-dimensional position-specific scoring matrix) program (Kelley et al., 2000, J. Mol. Boil. 299:499-520) to predict three dimensional structure of SS enzymes and the sequence comparisons revealed two distinct domains for each one of these enzymes (herein after referred to as “GLASS” and “GLYTR”). 3D-PSSM uses structural alignments of homologous proteins of similar three-dimensional structure in the structural classification of proteins (SCOP) database to obtain a structural equivalence of residues. These equivalences are used to extend multiply aligned sequences obtained by standard sequence searches. The resulting large-superfamily based multiple alignment is converted into a PSSM (position specific scoring matrix). Combined with secondary structure matching and solvation potentials, 3D-PSSM can recognize structural and functional relationships beyond state-of the-art sequence methods (Kelly et al., 2000, J. Mol. Biol. 299:499-520). Analysis through 3D-PSSM revealed a conserved two domain 3D structure for all maize starch synthases tested (FIG. 5). This is the first ever report in the scientific literature to model the 3D structure of any starch synthases. Using ProDom database of protein domain families available at the world widee web address Toulouse.inra.fr/prodom.html, Denyer et al., 2001; J. of Plant. Physiol. 158: 479-487, showed identity to three different domains in maize SS enzymes. However, they have not identified a function to Domain I and II and reported that Domain II is also found in Yeast α-amlyase. They reported Domain III as a putative glucosyl transferase domain, but neither provided detailed information as to which group in this family that maize SS enzymes fall under, nor sequence homology or models for 3D-structure of SS protein. Present invention provides the discovery of “GLYTR” domains for all these enzymes as a catalytic domain with significant alignments (using RPS-BLAST 2.2.2; oasis_sapv1.54 database and Domain architectural retrieval tool [DART]) to the glycosyl transferase group-1 domain otherwise referred to as the pfam00534 family (FIG. 6 & Table III). Members of this family are spread across about at least 20+ groups with different mechanism of glycosyl transferase function. Members of the pfam 00534 (PI00534) family transfer UDP, ADP, GDP or CMP linked sugars to a variety of substates including glycogen. The sequence in the catalytic or “GLYTR” domains is highly conserved in starch synthases (Table IV). Furthermore, the present invention relates to the identification of “GLucan ASSociation Domain (“GLASS” Domain), peptides and nucleic acids encoding the same. Glucan chain length specificity is conserved in “GLASS” domain of each form of starch synthase and glycosyl transferase function is conserved in “GLYTR” domain. In addition, starch entrapment function is also embedded in the “GLASS” domain of GBSS and SSI. Also, via genetic means, the present invention provides for generation of starch synthase(s) with novel functionalities by combining various domains from different synthases, i.e. by mixing and matiching functional “GLYTR” and “GLASS” domains from different organisms. Thus, the present invention in particular relates to modification to starch structure by increasing the association of SSIIa, SSIIb and Du1 with the starch granules especially, by engineering entrapment of their corresponding enzymes with the starch granules, and expression and entrapment of fusion proteins of SS enzymes, for example, catalytic domains of SSIIa, SSIIb and Du1 in association with glucan binding domains of GBSS or SSI in the starch granules to bring a change in the glucan chain lengths and distribution and thereby synthesize modified starch. The present invention provides modified plants which contain altered or modified starch synthase domains or polypeptide fusions expressed inside the amyloplast stroma and become associated with the starch granules of economically important crops like maize, potato, rice, oat, wheat, barley, sweet potato, cassaya, taro, sago, yam, banana, pea, etc. These SS enzyme fusions thus expressed will alter or influence the starch structure leading to plants with improved starch properties and modified starches with various industrial uses. Further applications and embodiments of this invention will be explained in detail herein below.

TARI F III
			%
			align-
ENZYME	Seq. ID No.	Alignment with Pfam 00534 (Glycosyl transferase, Group 1 domain)	ment
GBSS	SEQ ID NO:11	query:	378	NKEALQAEVGLPVDRNIPLVAFIGRLEEQKGPDVMAAAIPQLME-MVEDVQIVLLGTGKK	436	91.3
	(query)	Sbjct:	1	DREEIRKKLGIKEEKKI--ILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGG	58
	SEQ ID NO:12	Query:	437	KFERMLMSAEEKFPGKVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTP	496
	(snjct)	Sbjct:	59	EDELKLLALKLGLEDNVIFLGFVPDEDLPELYKSADVFVLPSRYEGFGIVLLEAMACGLP	118
		Query:	497	CACASTGGLVDTIIEGKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIK 545
		Sbjct:	119	VIATDVGGIPEIVKDGETGL----------LVEPGDVEALAEAIEKLLK 157
SSI	SEQ ID NO:13	Query:	441	LPIRPDVPLIGFIGRLDYQKGID-LIQLI--IPDLMREDVQFVMLGSGDPELEDWMRSTE	497	75
	(query)	Sbjct:	9	LGIKEEKKIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALK	68
	SEQ ID NO:14-	Query:	498	SIFKDKFRGWVGF-SVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATGGL	556
	(snjct)	Sbjct:	69	LGLEDNVI-FLGFVPDEDLPELYKSADVFVLPSRYEGFGIVLLEAMACGLPVIATDVGGI	127
		Query:	557	RDTVENFNPFGENG 570
		Sbjct:	128	PEIVKD----GETG 137
SSIIa	SEQ ID NO:15	Query:	540	LEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWIA---GQDVQLVMLGTGRADLERMLQHLE	596	84.3
	(query)	Sbjct:	9	LGIKEEKKIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALK	68
	SEQ ID NO:16	Query:	597	REHPNKVRGWVGFSVPMAHRIT--AGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	654
	(snjct)	Sbjct:	69	LGLEDNVI-FLGF-VPDEDLPELYKSADVFVLPSRYEGFGIVLLEAMACGLPVIATDVGG	126
		Query:	655	LRDTVAPFDPFGDAGLGWTFDRAEANKLIEALR 687
		Sbjct:	127	IPEIVKDGET------GLLVEPGDVEALAEAIE 153
SSIIb	SEQ ID NO:17	Query:	506	LQVRDDVPLIGFIGRLDHQKGVDIIADAIHWIA---GQDVQLVMLGTGRADLEDMLRRFE	562	87.2
	(query)	Sbjct:	9	LGIKEEKKIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALK	68
	SEQ ID NO:18	Query:	563	SEHSDKVRAWVGFS----VPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAV	618
	(snjct)	Sbjct:	69	LGLEDNVI-FLGFVPDEDLPELYKS---ADVFVLPSRYEGFGIVLLEAMACGLPVIATDV	124
		Query:	619	GGLRDTVAP------FDPFNDTGLGWTFDRAEAN 646
		Sbjct:	125	GGIPEIVKDGETGLLVEPGDVEALAEAIEKLLKD 158
DuI	SEQ ID NO:19	Query:	1478	PVVGIVTRLTAQKGIHLIKHAIHRTLERNGQVVLLGSAPDSRIQADFVNLANTLHGVNHG	1537	70.3
	(query)	Sbjct:	16	KIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALKLGLEDNV	75
	SEQ ID NO:20	Query:	1538	QVRLSLTYDEPLSHLIYAGSDFILVPSIFEPCGLTQLVAMRYGTIPIVRKTGGLFDTVFD	1597
	(snjct)	Sbjct:	76	IFLFGVPDEDLPEL--YKSADVFVLPSRYEGFGIVLLEAMACGLPVIATDVGGIPEIVKD	133
		Query:	1598	VDN 1600
		Sbjct:	134	GET 136

[0009]

TABLE IVa
PIR Multiple Alignment for “GLYTR” Domain of Maize SS enzymes
Enzyme
(res. #
of the	SEQ ID
start)	NO:.	CLUSTAL W (1.8) multiple sequence alignment
SSIIa-540	21	-----------EVRDDVPLLGFIGRLDGQKGVDIIGDAMPWIAG--QDVQLVMLGTG-----
		RADLERMLQHLEREHPNKVRGWVGFSVPMAHRITAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVGGLRDTVAPFDP-
		FGDAGLGWTFDRAEANKLIEALR---
SSIIb-506	22	----------LQVRDDVPLIGFIGRLDHQKGVDIIADAIHWIAG--QDVQLVMLGTG-----
		RADLEDMLRRFESEHSDKVRAWVGFSVPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAVGGLRDTVAPFDP-
		FNDTGLGWTFDRAEAN----------
SSI-441	23	-------- LPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLMR--EDVQFVMLGSG-----
		DPELEDWMRSTESIFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATGGLRDTVENFNP-
		FGENG---------------------
GBSS-378	24	NKEALQAEVGLPVDRNIPLVAFIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTG-----
		KKKFERMLMSAEEKFPGKVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIEGKTGFHMGR
		LSVDCNVVEPADVKKVATTLQ
Du1-1478	25	-----------------PVVGIVTRLTAQKGIHLIKHAIHRTLE--RNGQVVLLGSAPDS
		RIQADFVNLANTLHGVNHGQVRLSLTYDEPLSHLIYAGSDFILVPSIFEPCGLTQLVAMR
		YGTIPIVRKTGGLFDTVFDVDN--------------------------
		*::.:: **  *** .::   :       .:*.*:**:.        .:       .    .:.*  : :.
		.::* * **.*.: :.* ****** ** .*  ***      .*** **:   .
Footnote:
* = identical amino acid
:. = similar amino acids
- = residues left out

[0010]

TABLE IVb
PIR Multiple Alignment of SSI and SSII enzymes
ENZYME	CLUSTAL W (1.8) multiple sequence alignment	SEQ ID NO:
SSIIA.	MSSAAVSSSSSTFFLALASASPGGRRRARVGSSP---FHTGASLSFAFWAPPSPPRAPRD	26
SSIIB.	-MPGAISSSSSAFLLPVASSSPR-RRRGSVGAALRSYGYSGAELR-LHWARRGPPQD--G	27
SSI.	--MATPSAVGAACLLLARAAWP-----AAVG-----------DR-----ARPRRLQR---	29
	.: *: .:: :*   :: *     , **           .      *     :
SSIIA.	AALVRAEAEAGGKDAPPERSGDAARLPRARRNAVSKRRDPLQPVGRYGSATGNTARTGAA	26 (cont.d)
SSIIB.	AASVRAAAAPAGGESEEAAKSSSSSQAGAVQGSTAKAVDSASPPNPLTSAP---KQSQSA	27
SSI.	-VLRRRCVAELSREGPAPRPLPPALLA------P-----PLVP-----------------	29
	.  *  .   . :.       .:  .            .  *
SSIIA.	SCQNAALADVEIKSIVAAPPTSIVKFPAPGYRMILPSGDIAPETVLPAPKPLHESPAVDG	26 (cont.d)
SSIIB.	AMQNG------------------------------TSGGSSASTAAPVSGPKADHPSAPV	27
SSI.	-------------------------------------G-FLAPPAEPTGEPASTPPPVP-	29
	*   . .. *.  *    *..
SSIIA.	DSNGIAPPTVEPLVQEATWDFKKYIGFDEPDEAKDDSRVGADDAGSFEHYG-DNDSGPLA	26 (cont.d)
SSIIB.	TKREIDASAVKPEPAGDDARPVESIGIAEPVDAKADAAPATDAAASAPYDREDNEPGPLA	27
SSI.	-DAGLGDLGLEPE------------GIAEG--SIDNTVVVASEQDSEIVVGKEQARAKVT	29
	.  :    ::*             *: *   :  ::   :.   *      ::  . ::
SSIIA.	GENVMNVIVVAAECSPWCKTGGLGDVVGALPKALARRGHRVMVVVPRYG------DYVEA	26 (cont.d)
SSIIB.	GPNVMNVVVVASECAPFCKTGGLGDVVGALPKALARRGHRVMVVIPRYG------EYAEA	27
SSI.	----QSIVFVTGEASPYAKSGGLGDVCGSLPVALAARGHRVMVVMPRYLNGTSDKNYANA	29
	.::.*:.*.:*:.*:****** *:** *** ********:***       :*.:*
SSIIA.	FDMGIRKYYKAAGQDLEVNYFHAFIDGVDFVFIDAPLFRHRQDDIYG---GSRQEIMKRM
SSIIB.	RDLGVRRRYKVAGQDSEVTYFHSYIDGVDFVFVEAPPFRHRHNNIYG---GERLDILKRM
SSI.	FYTEKHIRIPCFGGEHEVTFFHEYRDSVDWVFVDHPSY-HRPGNLYGDKFGAFGDNQFRY
	:      * : **.:** : *.**:**:: * : ** .::**   *   :   *
SSIIA.	ILFCKVAVEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDHGLMQYTRSVLV	26 (cont.d)
SSIIB.	ILFCKAAVEVPWYAPCGGTVYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMQYARSVLV	27
SSI.	TLLCYAACEAPLILELGGYIYG-QNCMFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILV	29
	*:* .* *.*     **  **  * :*:.****::*:** * * **  *: : :**:**
SSIIA.	IHNIAHQGRGPVDEFPYMDLP-------EHYLQHFELYDPVG-GEHANIFAAGLKMADRV	26 (cont.d)
SSIIB.	IHNIABQGRGPVDDFVNFDLP-------EHYIDHFKLYDNIG-GDHSNVFAAGLKTADRV	27
SSI.	IHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHALDKGEAVNFLKGAVVTADRI	29
	***:****  *.. :  :.**       *  : .:   . :. *:  *.: ..:  ***:
SSIIA.	LETLDAGKRQCKAALQRELGLEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWIAGQDVQLV	26 (cont.d)
SSIIB.	FETLDTGKRQCKAALQRQLGLQVRDDVPLIGFIGRLDHQKGVDIIADAIHWIAGQDVQLV	27
SSI.	VDDLS-GKAKCKGALQKELGLPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLMREDVQFV	29
	: *. ** :**.***::*** .* ****:******* ***:*:*   :  :  :***:*
	MLGTGRADLERMLQHLEREHPNKVRGWVGFSVPMAHRITAGADVLVMPSRFEPCGLNQLY	26 (cont.d)
SSIIA.	MLGTGRADLEDMLRRFESEHSDKVRAWVGFSVPLAHRITAGADILLMPSRFEPCGLNQLY	27
SSIIB.	MLGSGDPELEDWMRSTESIFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLY	29
SSI.	***:* .:**  ::  *  . *.*.
	*******::*******.*::**************
SSIIA.	AMAYGTVPVVHAVGGLRDTVAPFDPFGDAG---	26 (cont.d)
SSIIB.	LGWTFDRAEANKLIEALRHCLDTYRKY	27
SSI.	AMAYGTVPVVHAVGGLRDTVAPFDPFNDTG---	29
	LGWTFDRAEANRMIDALSHCLTTYRNY
	AMQYGTVPVVHATGGLRDTVENFNPFGENGEQGTGWAFAPLTTENMFVDIANCN-----
	-
	** *********.*******  *:**.: *    **:*    ::.::  : :*
SSIIA.	GESWKSLQARGMSQDLSWDHAAELYEDVLVKAKYQW	26 (cont.d)
SSIIB.	KESWRACRARGMAEDLSWDHAAVLYEDVLVKAKYQW	27
SSI.	------IYIQGTQVLLGRANEARHVKRLHVGPCR--	29

[0011]

TABLE V
Possible, but not limited to Amino acid Sequences for Some of the
Proposed Fusion Proteins
A GBSS(61-300) + Du1 (1201-1674)
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTS SEQ ID NO:30
VVSEIKMGDG YETVRFFHCY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAL
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN WMKAGILEAD RVLTVSPYYA EELISGIARG CELDNIMRLT
GITGIVNGMD VSEWDPSRDK GGIYDNRNGL DYHIPVFGSI AKEPPMHIVH IAVEMAPIAK VGGLGDVVTS
LSRAVQDLGH NVEVILPKYG CLNLSNVKNL QIHQSFSWGG SEINVWRGLV EGLCVYFLEP QNGMFGVGYV
YGRDDDRRFG FFCRSALEFL LQSGSSPNII HCHDWSSAPV AWLHKENYAK SSLANARVVF TIHNLEFGAH
HIGKAMRYCD KATTVSNTYS KEVSGHGAIV PHLGKFYGIL NGIDPDIWDP YNDNFIPVHY TCENVVEGKR
AAKRALQQKF GLQQIDVPVV GIVTRLTAQK GIHLIKHAIH RTLERNGQVV LLGSAPDSRI QADFVNLANT
LHGVNHGQVR LSLTYDEPLS HLIYAGSDFI LVPSIFEPCG LTQLVAMRYG TIPIVRKTGG LFDTVFDVDN
DKERARDRGL EPNGFSFDGA DSNGVDYALN RAISAWFDAR SWFHSLCKRV MEQDWSWNRP ALDYIELYRS
ASKL
B. GBSS (61-300) + 551 (400-622)
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTS SEQ ID NO:31
VVSEIKMGDG YETVRFFHCY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAL
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN KATTVSNTYS KEVSGHGAIV PHLGKFYGIL NGIDPDIWDP
NGIDINDWNP ATDKCIPCHY SVDDLSGKAK CKGALQKELG LPIRPDVPLI GFIGRLDYQK GIDLIQLIIP
DLMREDVQFV MLGSGDPELE DWMRSTESIF KDKFRGWVGF SVPVSHRITA GCDILLMPSR FEPCGLNQLY
AMQYGTVPVV HATGGLRDTV ENFNPFGENG EQGTGWAFAP LTTENMFVDI ANCNIYIQGT QVLLGRANEA
RHVKRLHVGP CR
C. GBSS (61-300) + SSIIa (481-732)
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTS SEQ ID NO:32
VVSEIKMGDG YETVRFFHCY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAL
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN DIIRSNDWKI NGIVNGIDHQ EWNPKVDVHL RSDGYTNYSL
ETLDAGKRQC KAALQRELGL EVRDDVPLLG FIGRLDGQKG VDIIGDAMPW IAGQDVQLVM LGTGRADLER
MLQHLEREHP NKVRGWVGFS VPMAHRITAG ADVLVMPSRF EPCGLNQLYA MAYGTVPVVH AVGGLRDTVA
PFDPFGDAGL GWTFDRAEAN KLIEALRHCL DTYRKYGESW KSLQARGMSQ DLSWDHAAEL YEDVLVKAKY
QW
D. GBSS (61-300) + SSIIb (481-698)
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTS SEQ ID NO:33
VVSEIKMGDG YETVRFFHCY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAL
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN YTNYTFETLD TGKRQCKAAL QRQLGLQVRD DVPLIGFIGR
LDHQKGVDII ADAIHWIAGQ DVQLVMLGTG RADLEDMLRR FESENSDKVR AWVGFSVPLA HRITAGADIL
LMPSRFEPCG LNQLYAMAYG TVPVVHAVGG LRDTVAPFDP FNDTGLGWTF DRAEANRMID ALSHCLTTYR
NYKESWRACR ARGMAEDLSW DHAAVLYEDV LVKAKYQW

SUMMARY OF THE INVENTION

[0012] The invention provides the polypeptide sequence of GBSS enzymes (FIGS. 9A & 9B) that will enable the fused polypeptides of other starch synthases to become entrapped in the starch granules and be functional. The present invention provides modified starches with altered glucan chain lengths and a variety of starch synthase polypeptide domain fusions (TABLE V) to produce the same, as well as gene constructs that encode such fusions and in methods for the transformation of plants using such constructs as well as in the transformed plants thus obtained. The present invention also relates to the expression in plants of polypeptides-including SS enzymes as fusion proteins with improved affinity to starch and modified catalytic capabilities and to the in vivo and in vitro synthesis of glucan chains of modified lengths as compared to a plant producing native starch or starch produced with native starch synthases. In particular, the invention relates to the expression in plants of soluble starch synthase protein domains and/or polypeptide domains as fusion peptides with starch association domain of GBSS or SSI or any other SS enzyme. According to this invention, GBSS is any fusion protein thus generated using GBSS, for example, any SS or any other enzyme domain plus GLASS domains of GBSS and may include GLYTR domain as well. SS or Starch synthase means any starch synthesis enzyme that is present in soluble form, for eg. SSI, SSIIa, SSIIb, and SSIII.

[0013] The present invention provides a method for obtaining transformed plants that produce starches with modified glucan chain lengths. A farther object of this invention is to express the desired starch synthases or polypeptides in plants with modified functionalities in vivo and in association with starch or starch granules in order to introduce a desired modification in the average chain length of amylopectin.

[0014] The above objects are achieved by expressing a desired fusion protein of starch synthase or polypeptide that can interact with starch or starch granule in bringing a modification of glucan chain lengths.

[0015] By “interact with starch or starch granules” is generally meant that the fusion protein of starch synthases can modify, alter the chain length distribution of starch or modify the fine structure of starch. This interaction will result in starch or starch granules that differ from the naturally occurring plant starch in at least one property thereof, for example, glucan chain lengths, glucan composition, crystallinity, branching degree etc. Therefore, starch synthase fusion protein will influence at least one physical or chemical property of the starch.

[0016] Broadly, the invention relates to a method for expressing fusion proteins consisting of a desired one or more catalytic domains (“GLYTR” Domain) of one or more starch synthase or any other enzyme in association with glucan association domain (“GLASS” Domain) of GBSS or similar enzyme.

[0017] In addition, the invention also relates to a method for expressing fusion proteins consisting of a desired one or more catalytic domains (“GLYTR” Domain) of one or more starch synthase or any other enzyme in association with glucan association domain (“GLASS” Domain) of SSI or other similar enzymes.

[0018] The invention also relates to a method for expressing fusion proteins consisting of a desired domain (“GLASS” Domain) of any starch synthase enzyme from any organism fused with another desired domain of another starch synthase enzyme (“GLYTR” Domain) from the same or any other organism in any combination and vice versa.

[0019] The starch synthase protein domains or polypeptides expressed via the method of invention can be from any plant or from any plant part including seeds, leaves, roots, tubers, stems, stalks, fruits, and/or flowers. The starch synthase polypeptides thus expressed may or may not by themselves have natural affinity for starch or starch granules, and the method of the invention is used to provide a polypeptide of GBSS or SSI with such affinity.

[0020] Furthermore, the starch synthase polypeptides thus expressed may not by themselves have the natural affinity for starch or starch granules, and the method of invention is used to provide a polypeptide of SSI with such affinity.

[0021] The transformants of the invention expressing the starch synthase fusion proteins, may change the starch structure in different forms. For example, the starch synthases of the invention can change any one or the more of crystallinity of said starch, can change the glucan content, degree of branching, and especially the length of glucan chains in the amylopectin molecule.

[0022] The above modification in the glucan chain length distribution can bring changes in the affinity of the starch synthase enzymes that are intrinsic to the starch granule. And the change can increase or decrease the association of intrinsic starch synthase enzymes, like SSI and GBSS, to the starch granules.

[0023] Therefore, a further aspect of the invention relates to a method for providing a recombinant protein or polypeptide with affinity for starch granules and that has catalytic activity in order to bring changes in the structure of the starch.

[0024] The genes encoding the desired starch synthase polypeptide sequence may be derived from any source, including plants, animals, fungi, algae, yeasts, bacteria, and any other microorganisms. The expressed genes may be homologous or heterologous to the starch producing plant in which the fusion peptides of starch synthase are expressed.

[0025] A further aspect of the invention is that the genes encoding any of the starch synthase fusion polypeptides can be variants or mutants of such proteins, such as those known in the art and/or obtainable via genetic manipulations. This includes mutant enzymes with biological activity but, with altered properties in terms of altered substrate binding activity, altered substrate specificity, and finally altered kinetic properties.

[0026] In another aspect the present invention provides expression of fusion proteins with of the invention is that the expression of fusion proteins with the starch association domain of SSI and/or GBSS (“GLASS” Domain) which may include partial or full length catalytic domains of any starch synthases, starch branching enzymes, debranching enzymes, disproportionating enzymes, kinases, phosphorylases and any of the isoforms of above enzymes.

[0027] More in particular, the expression of these starch synthase fusion proteins along with the starch association domain of GBSS will lead to a “modified-starch”, the subject matter of invention.

[0028] The said modified starch may be further modified according to the techniques known to the skilled person. Whether in modified or unmodified form, the starch will be used for food and non-foodstuff.

[0029] The above “modified starch” resulting from the expression of fusion proteins of starch synthases will have at least one of the listed below altered or improved properties as compared to the natively produced starch by a plant. The modified starch will have an altered or improved morphology, retrogradation, waterbinding or swelling potential of the granules, gel strength, adhesiveness, cohesiveness, hardness, elasticity, increased or decreased granule size, degree of branching, crystallinity, degree of cross-linking, and increased or decreased glucan chain lengths.

[0030] The present invention further provides the following method of:

[0031] a) providing a genetic construct containing at least one or more nucleotide sequence encoding desired polypeptide sequence containing one or more catalytic domains (“GLYTR” Domain) of starch synthase fusion protein combined with at least one nucleotide sequence encoding starch association domain (“GLASS” Domain) of GBSS or SSI;

[0032] b) providing a genetic construct containing at least one or more nucleotide sequence encoding desired polypeptide sequence(s) containing at least one domain (“GLYTR”or “GLASS” Domain) of one starch synthase with at least one nucleotide sequence encoding desired polypeptide sequence of another domain (“GLYTR”or “GLASS” Domain) of another starch synthase and vice versa;

[0033] c) transforming a plant with any of the above construct(s); and

[0034] d) expressing the genetic construct in the plant in vivo.

[0035] The present invention provides an isolated DNA molecule encoding a fusion protein consisting of four different functional domains selected from the group consisting of GLASS, LINKR, GLYTR, and CTEND which are operably linked to one another. The isolated DNA molecule of the present invention may contain, for example, a GLASS domain which contains a GBSS GLASS. Further, the GBSS GLASS of the present invention may contain a GLASS of SEQ ID NO: 1. Alternatively, the isolated DNA molecule of the present invention may contain a GLASS domain which contains a SSI GLASS. The SSI GLASS of the present invention may contain, for example, a GLASS of SEQ ID NO: 2. Moreover, the isolated DNA molecule of the present invention may contain a GLASS domain which contains a SSII GLASS. For example, the SSII GLASS of the present invention may ontain a GLASS of SEQ ID NOs: 3 and/or 4. Furthermore, the isolated DNA molecule of the present invention may contain a GLASS domain which contains a SSIII GLASS. The SSIII GLASS of the present invention may further contain a GLASS of SEQ ID NO:5

[0036] In one embodiment, the isolated DNA molecule of the present invention contains at least one of a GBSS GLASS, a SSI-GLASS, a SSII-GLASS or a SSII-GLASS wherein the GLASS or GLASS domain are a GLASS or GLASS domain of a glucan producing organism or at least 80% (preferably at least 85%, more preferably at least 90%, alternatively at least 95%, or at least 98%) identical or similar to a GLASS or GLASS domain peptide of a glucan producing organism.

[0037] The present invention further provides an isolated DNA molecule, as described herein and above wherein the LINKR domain is a GBSS-LINKR, a SSI-LINKR, a SSII-LINKR and/or a SSIII-LINKR. The LINKR of the invention may contain a LINKR sequence containing any of SEQ ID NOs:121-171; 336-386;527-577; 733-783; and/or 983-1033.

[0038] The present invention further provides an isolated DNA molecule, as described herein wherein the GLYTR domain may contain a GBSS-GLYTR, a SSI-GLYTR, a SSII-GLYTR and/or a SSIII-GLYTR. More specifically, the GLYTR domain of the present invention may contain a GLYTR sequence containing at least one of SEQ ID NOs:1136, 1137, 1138, 1139, and/or 1140. Alternatively, the GLYTR of the present invention may contain a GLYTR sequence containing at least one of SEQ ID NOs: 172-222; 387-437; 578-628; 784-834; and/or 1034-1084.

[0039] The present invention further provides an isolated DNA molecule, as described herein and above wherein the CTEND domain is a GBSS-CTEND, a SSI-CTEND, a SSII-CTEND and/or a SSIII-CTEND. The CTEND of the invention may contain a CTEND sequence containing any of SEQ ID NOs: 1146, 1147, 1148, 1148, 1149 and/or 1150. Alternatively, the CTEND of the present invention may contain a CTEND sequence containing a CTEND sequence containing at least one of SEQ ID NOs:223-266; 438-461; 629-676; 835-882; and/or 1085-1135.

[0040] The present invention provides an isolated DNA molecule encoding a fusion peptide which contains a GBSS GLASS domain operably linked to a LINKR and a catalytic domain from a functional protein that synthesizes an ∀-1,4 glucan or an ∀-1,3 glucan, or an ∀-1,6 glucan, wherein the fusion peptide is capable of modifying the glucan structure of a starch producing organism when starch is produced by such an organism or part thereof in the presence of a fusion peptide of the present invention.

[0041] In a further embodiment, the present invention provides a DNA molecule which encodes a fusion peptide, and a fusion peptide coded for by the same, wherein the GLASS and/or LINKR sequence contained therein contains at least one of SEQ ID NOs:75-120; 284-335, 475-526; 682-732; 933-982 and/or 121-171, 336-386, 527-577, 733-783, and 983-1033.

[0042] In a further embodiment, the present invention provides an isolated DNA molecule encoding a polypeptide, and a polypeptide so encoded, with glucan association properties of a maize GBSS enzyme, and being capable of modification of starch metabolism in a plant or plant cell, the DNA containing a molecule of, for example, at least one of the following:

[0043] (a) a DNA molecule encoding a protein domain having the amino acid SEQ ID NO:1;

[0044] (b) a DNA molecule containing a corresponding nucleotide sequence from SEQ ID No:1141;

[0045] (c) a DNA molecule containing a nucleotide sequence differing from the sequence of the DNA molecules of (a) or (b) due to the degeneracy of the genetic code;

[0046] (d) a DNA molecule containing a DNA sequence which hybridizes to any one of the DNA molecules of (a), (b) or (c) or fragment thereof, and which is equal to or more than 80% homologous or identical or similar to the DNA molecule of (a), (b), or (c), or fragment thereof, wherein the DNA sequence encodes a polypeptide with Glucan Association Domain (Domain A) of a GBSS enzyme.

[0047] The present invention provides an isolated DNA molecule encoding a polypeptide with a glycosyl transferase function of a soluble or granule bound maize SS enzymes capable of modifying starch metabolism in a plant or plant cell, the DNA molecule containing, for example, at least one of the following:

[0048] (a) a DNA molecule encoding a protein domain containing an amino acid of SEQ ID NOs:1, 2, 3, 4, and 149;

[0049] (b) a DNA molecule containing the corresponding nucleotide sequence of SEQ ID NOs: 1141, 11142, 1143, 1144, and 1145;

[0050] (c) a DNA molecule containing a nucleotide sequence differing from the sequence of the DNA molecules of (a) or (b) due to the degeneracy of the genetic code,

[0051] (d) a DNA molecule containing a DNA sequence which hybridizes to any one of the DNA molecules of (a), (b) or (c), or fragment thereof, and which is equal to or more than 80% homologous or identical to the DNA molecule of (a), (b), or (c), or fragment thereof, wherein the DNA sequence encodes a polypeptide with a glycosyl transferase domain of a SS enzyme.

[0052] The present invention provides a recombinant or isolated DNA molecule, as described, containing a maize GBSS nucleotide coding region encoding for an amino acid sequence of SEQ ID NOs: 101-146 fused with a corresponding coding region of a maize SS enzyme that encode for an amino acid sequence containing any of SEQ ID NOs: 35-74; 121-171; 172-222; 223-266; 268-283; 284-335; 336-386; 387-437; 438-461; 463-474; 475-526; 527-577; 578-628; 629-676; 678-681; 682-732; 733-834; 835-882; 884-932; 933-982; 1034-1084; and/or 1085-1135.

[0053] The present invention provides a recombinant or isolated DNA molecule, as described, containing a GLYTR, LINKER or CTEND domain DNA sequence containing any of SEQ ID NOs: 172-222; 387-437; 578-628; 784-834; 1034-1084, 121-171; 336-386; 527-577; 733-783; 983-1033 or 223-266; 438-461; 629-676; 835-882; 1085-1135 operably linked in any order with a corresponding DNA sequence that encodes for a glucan association domain containing any of SEQ ID NOs: 75-120; 284-335; 475-526; 682-732; and/or 933-982.

[0054] The present invention provides a recombinant or isolated DNA molecule, as described, further containing a DNA sequence differing from the sequence of any of the DNA molecules of SEQ ID NOs: 34-1150 due to the degeneracy of the genetic code, and/or protein or polypeptide originating from a different source, such as a plant species other than plant species such as maize, bacteria (e.g. E. Coli), Yeast, algae (Chlamydomonas), or fungus.

[0055] The present invention provides a recombinant or isolated DNA molecule, as described herein, wherein the DNA sequence contains at least one coding region of a glucan association domain of SEQ ID NOs:75-120; 284-335; 475-526; 682-732; and/or 933-982 fused with a coding region of any glucan transferases listed in Table XXXVII.

[0056] The present invention further provides a method of expressing a starch synthase fusion proteins or polypeptides in a plant, in which the starch synthase protein or polypeptide domains are expressed as a fusion with a glucan association domain of granule bound starch synthase. Theprotein or polypeptide of the method of the invention may be heterologous with respect to the plant in which the fusion is expressed.

[0057] The present invention further provides a method, as described herein, wherein th method involves the steps of:

[0058] providing a genetic construct containing at least one nucleotide sequence encoding the desired protein domain or polypeptide domain combined with at least one nucleotide sequence encoding a glucan association domain of GBSS, so that the construct encodes a fusion of the desired protein/polypeptide and at least one glucan association domain;

[0059] transforming a plant with the genetic construct;

[0060] expressing the genetic construct in the plant.

[0061] The present invention further provides a method, as described herein, in which the protein or polypeptide or recombinant protein or recombinant polypeptide is an enzyme. Such an enzyme of the present invention may, for example, be an enzyme which is an enzyme that can interact and associate with starch or starch granules, or facilitate or be entrapped in starch or starch granules, and is capable of at least one of modifying, increasing, decreasing, altering or influencing starch structure or starch synthesis.

[0062] The present invention further provides a vector containing a DNA molecule as provided herein. The vectors of the present invention may contain, for example, a DNA molecule which is linked in the sense orientation to DNA elements ensuring transcription of a translatable RNA in a prokaryotic or an eukaryotic cell.

[0063] The present invention further provides a host cell containing a vector of the present invention.

[0064] In a further embodiment, the present invention provide a plant cell containing a DNA molecule of the present invention linked to a heterologous promoter.

[0065] The present invention further provides a plant containing a plant cell of the present invention. The plants according to the present invention may be, for example, a cereal, such as maize, rice, wheat, barley, oats, or a root crop, such as potato, sweet potato, cassaya, yam, taro, or other starch producing plant, such as peas or banana. Moreover, the plants of the present invention may contain or produce starch or starch granules in at least one of its parts, including its seeds, leaves, roots (tubers), tubers, stems, stalks, fruits, grains or flowers. Furthermore, the plants of the present invention include elements containing a homologous or heterologous promoter specific for expression of said DNA molecule in the at least one of its parts.

[0066] The present invention provides seeds from the plant of the present invention, which are preferably capable of expressing the recombinant molecule or DNA molecule of the present invention.

[0067] The present invention provides amodified starch derived from cells of a plant or plant part of the present invention.

[0068] In a further embodiment, the present invention provides a food or feed containing a modified starch of the present invention or plant or plant part of the present invention.

BRIEF DESCRIPTION OF THE FIGURES

[0069] FIG. 1. Shows 14C-ADPG incorporation as dpms (disintegrations per minute) into different glucan chain lengths separated on Sepharose CL-6B Column by various starch synthase enzymes from maize. The glucan (potato amylopectin and glycogen) was debranched after SS enzyme reaction and prior to running on the column. Shown in the order (from top to bottom of the figure) are, GBSS (granule bound starch synthase), Du-1 (SSIII), soluble starch synthase IIa, soluble starch synthase IIb (IIb), and soluble starch synthase I (SSI). Dp=degree of polymerization or number of glucose units. There is a significant difference in the chain length specificity of various enzymes. For example, GBSS incorporated most of the 14C-ADPG into very long glucan chains that are more than 30 units long. Du-I or SSIII incorporated more than half the label into glucan chains that are in between dp 20 and 30. SSIIa and SSIIb incorporated most of the 14C-ADPG into glucan chains that are shorter than 20. Most of the 14C-ADPG incorporation by SSI was into the glucan chains that are shorter than dp 10. Therefore, there are four distinct classes of starch synthases with differences in chain length specificity that are detected in maize endosperm.

[0070] FIG. 2. Shows results from Sepharose CL-6B chromatography of debranched products of GBSS and SSI. The figure displays clear distinction in the chain length specificities of GBSS and SSI enzymes in that 14C-labeled products of GBSS elute much earlier than the 14C labeled products of SSI. This means that GBSS elongates longer glucan chains, where as SSI elongates shorter glucan chains.

[0071] FIG. 3. Shows results from thin layer chromatography of debranched glycogen after 14C-ADPG incorporation into various glucan chains by different starch synthase enzymes in maize. Panel on the left shows the carbohydrate staining and panel on the right shows 14C-label incorporation into different glucan chain lengths. Carbohydrate staining shows that there is equal amount of carbohydrate loaded in each well. Also, there is equal amount of carbohydrate visible in each glucan class. However, panel on the right shows that each enzyme picked a different glucan class for 14C-ADPG incorporation. The numbers on the left indicate the size of the glucan in each class. Maltooligosaccharide (MOS) ladder (of known sizes) as a marker was run in order to enable us to estimate the glucan chains in each lane. The numbers 1-7 on the left panel indicate the number of glucoses. The numbers on the far left indicate the glucan chain (8-13) lengths interpreted based on the MOS ladder. The right panel shows that GBSS and Du-1 incorporated 14C-ADPG mostly into glucan chains longer than dp 13. Contrarily, SSI incorporated most of the 14C-ADPG into glucan chains that are dp 8 or 9. SSIIa incorporated most of the 14C-ADPG into glucan chains that are dp 8. SSIIb incorporated most of the 14C-ADPG label into glucan chains that are dp 11 and 12. Therefore, there appears to be a chain length specificity for each SS enzyme.

[0072] FIG. 4. Shows SDS-Page of proteins associated with the starch granules of maize kernels. Proteins from starch granules were extracted by boiling and ran on 10% polyacrylamide gels. Proteins were stained with coomassie blue. The figure shows that majority of the protein entrapped in the starch granules is GBSS and there is some SSI and branching enzymes as well.

[0073] FIG. 5. Shows proposed model for starch synthases based on 3D-PSSM automated fold recognition technique (Kelley et al., 2000). All the five known starch synthases from maize have two distinct domains with a linker in between. The labels on the top show the corresponding names of these domains based on the functionality disclosed in the present application. “GLASS” stands for glucan association domain and “GLYTR” stands for glycosyl transferase domain. “GLASS” and “GLYTR” are linked to each other by “LINKR” sequence.

[0074] GBSS is shown in FIGS. 5A-1, upper left panels.

[0075] SS1 is shown in FIGS. 5A-2, upper right panels.

[0076] SSIIa is shown in FIGS. 5A-3, lower left panels.

[0077] SSIIb is shown in FIGS. 5A-4, lower right panels.

[0078] DU1 is shown in FIGS. 5B-1 (FIG. 5. Cont.d).

[0079] FIG. 6. Is a cartoon showing the location of Glycosyl transferase group 1(Pfam 00534) domain of maize starch synthases.

[0080] FIG. 7. Shows a picture of affinity gel electrophoresis to determine glucan association peptide of GBSS.

[0081] Panel 1=Native gel containing 0.2% potato amylopectin. It shows GBSS has strong affinity to amylopectin.

[0082] Panel 2=GBSS enzyme was digested into various peptides using Endo-Glu-C or V8 enzyme. The peptides were separated on 10% SDS-PAGE gels and visualized by using silver staining of peptides.

[0083] Panel 3=Purified GBSS enzyme on 10% SDS-PAGE gels.

[0084] Panel 4=V8 enzyme peptides that were bound to amylopectin in the native gels were excised and ran on 10% SDS-gels. The arrows indicate the peptides that had affinity to glucan.

[0085] Panel 5=A renaturing gel for detecting the activity of SS enzymes. The smallest peptide from FIG. 4 of the above was blotted onto PVDF membrane. The amino acid sequence of the peptide is as follow.

KIYGPVAGTDYRDNQL RFSLLCQAAL EAPRILSLNN NPYFSGPYGE
DVVFVCNDWHTGPLSCYLKSNYQSHGIYRD AKTAFCIHNI
SYQGRFAFSD YPELNLPERF
KSSFDFIDGYEKPVEGRKINWMKAGILEAD RVLTVSPYYA EE

[0086] FIG. 8 shows the effect of increasing avg. OCL of glycogen on the affinity (1/Kd) (graphs, A, B, C) and catalytic activity (graph D) of the SSI-2 enzyme.

[0087] FIG. 8A shows the effect of increasing avg. OCL of glycogen on the affinity (1/Kd);

[0088] FIG. 8B shows the effect of increasing avg. OCL of glycogen on the affinity (1/Kd);

[0089] FIG. 8C shows the effect of increasing avg. OCL of glycogen on the affinity (l/Kd); and

[0090] FIG. 8D shows the catalytic activity of the SSI-2 enzyme. The graphs shows increased affinity and decreased enzyme activity with increase in the average outer chain length of the substrate molecule. Graph 3B (8B) shows how the affinities of amylose, amylopectin and starch fall within the same range of modified glycogens with extended OCL and also shows upward trend in affinity after dp of about 17. Graph 3D (8D) shows the contrasting results with the enzyme activity using modified glycogens with extended OCLs. Data are average of three separate replications±SE.

[0091] FIG. 9. Shows a comparison of mobility of SSI and SSI-2 proteins in the substrate containing native gels.

[0092] FIG. 9A.

[0093] SSI and SSI-2 proteins were run in the native gels containing either none (panels 1 and 2) or 2% starch (panels 3 and 4), or 2% glycogen (panels 5 and 6). The gels were stained for activity using I2 solution (for details, see materials and methods section). The arrows indicate the binding of protein to the substrate in the gels containing 2% starch.

[0094] FIG. 9B.

[0095] Panels 2A, 2B, and 2C are coomassie staining, and panels 3A, 3B, and 3C are activity staining of the above proteins. Panels 2A, 2B, 3A, and 3B are native gels containing 2% starch and panels 2C and 3C are renaturing gels (see materials and method section for details). The gels show V8 peptide(s) of SSI (2A, 3A, 2C, and 3C) and SSI-2 (2B, 3B, 2C and 3C) that were bound to the substrate in the native gels (2A, 3A, 2C), and were active (2B, 3B, 3C). The arrows indicate the protein or peptide(s) bound to the substrate in the gels right in the well itself. For panels, 2C and 3C, molecular weight markers were shown on the left in kD.

[0096] FIG. 10. Shows a comparison of the elution profile of 14C-labeled glucans on Sepharose CL-4B column.

[0097] FIG. 10A shows debranched products of SSI reaction using unmodified glycogen.

[0098] FIG. 10B shows the results with modified glycogen (OCL=14-15).

[0099] Both were run on a Sepharose CL-4B. At the end of the enzyme reaction, carbohydrate was subjected to Isoamylase digestion (as described in the materials and methods section). Open squares are elution of total carbohydrate as absorbance at 490 nm of fractions mixed with phenol and H2SO4; open triangles are the elution profile of 14C-labeled products. The scale bar for each graph shows profile of elution of corresponding chain lengths of debranched amylopectin ran on the same column. Each data point is an average of 3 separate runs on the columns.

[0100] FIG. 11.

[0101] FIG. 11A shows increased affinity and increased enzyme activity of GBSS with increase in the average outer chain length of the substrate molecule.

[0102] FIG. 11B shows the contrasting results with the enzyme activities of GBSS and SSI enzyme using modified glycogens with extended OCLs. Data are average of three separate replications±SE.

[0103] FIG. 12. Shows a comparison of the glucan binding affinities of SSIIa, SS1-2, and GBSS enzymes. Affinity is calculated based on the molar availability of outer chain lengths.

[0104] FIG. 12A shows increase in the affinity of GBSS and SSI-2 enzymes to increase in the outer chain length of modified glycogen up to dp ˜14 to 16. To the same increase in the outer chain lengths, SSIIa did not show any increase in the affinity.

[0105] FIG. 12B shows a linear increase in the affinity of GBSS to further increments in the chain length whereas, SSIIa did not show any increase in the affinity.

[0106] It is interesting to note that SSI-2 displayed more than 4000 fold increase in the affinity when the length of the outer chains was extended on an average up to 21glucose units.

[0107] FIG. 13. Shows summary of activities of SSI, SSIIa, SSIIb, SSIII (DuI) and GBSS using chain extended glycogen. Based on the observations in this figure, the present invention classifies maize a-1,4 glucan transferases or starch synthases based on their specificities to process various lengths of glucan chains in the amylopectin cluster. For example, according to the present invention, ‘Class I’ enzymes that include maize SSI and like enzymes, and preferentially elongate a-1,4 glucan chains to synthesize shorter A and BI chains; ‘Class II’ enzymes that include maize SSIIa and SSIIb and like enzymes, and preferentially add a glucose unit(s) to a-1,4 glucan chains to synthesize longer A and B 1 chains and intermediate B2 or B3 chains; ‘Class III’ enzymes that include maize SSIII and GBSS, and preferentially add a glucose unit(s) to a-1,4 glucan chains to synthesize longer A, B 1, B2 and B3 chains as well as longer B3 or C chains of amylopectin. In maize or any other crop when transformed to express or over express any one specific class of starch synthases described above will result in an increased number of glucan chains in that specific class.

[0108] FIG. 13A shown A similarity in Chain Length Specificities of Du-1 and SSIIa;

[0109] FIG. 13B shows A Comparison of Chain Length Specificities of SSI-2 and SSIIb;

[0110] FIG. 13C shows A Comparison of Contrasting Catalytic Activities of GBSS and SSI to Increasing Gluican Chain Lenghs of Glycogen.

[0111] FIG. 14.

[0112] FIG. 14A shows detection of the expression of fusion proteins in the soluble extracts of transgenic maize kernels. The transgenic proteins are expressed in the soluble extracts.

[0113] FIG. 14B shows detection of the transgenic fusion protein only in the 210 and 218 (See example number I for details).

[0114] FIG. 15.

[0115] FIG. 15A shows the detection of transgenic citrate synthase protein in the soluble extracts of maize kernels. However, the protein did not get associated with the starch granules.

[0116] FIG. 15B shows activities of citrate synthase from transgenic maize kernels.

[0117] FIG. 15C (right panel) shows Western blotting of Transgenic Starch-granule proteins using GFP antibody.

[0118] FIG. 16. Shows the differences in the models generated by 3D-PSSM for different proteins. Glycogen phosphorylase from E. Coli folds very differently as compared to SS enzymes and epimerase. It confirms that all SS enzymes have a similar 2 domain but functionally different 3D structures.

[0119] FIG. 16A shows UDP-N-Acetylglucosamine 2-epimer;

[0120] FIG. 16B shows T4 phage B-glycosyltransferase;

[0121] FIG. 16C shows Glycogen phosphorylase from E. coli.;

[0122] FIG. 16D shows how the catalytic or GLYTR domains of SS enzymes fold very similar to pfam 00534 structure. FIG. 16D also shows how the glucans or glucan chains are held within the groove.

[0123] FIG. 17. Shows 3D structures of some of the proposed fusion proteins.

[0124] FIG. 17A (upper left) shown GBSS+SSIIA;

[0125] FIG. 17B (upper right) shows GBSS+SSIIB;

[0126] FIG. 17C (lower left) shows GBSS+SSI; and

[0127] FIG. 17D (loer right) shows GBSS+DuI.

[0128] FIG. 18. Shows SDS-electrophoresis and coomassie staining of proteins from various plants, namely banana fruit, basella leaf, carrot root, maize endosperm, green bean pods, rice endosperm, rutabaga root, sweet potato root, and wheat endosperm. The proteins were run on native gel containing 2% boiled starch. The peptides or proteins that were bound to the glucan in the well were visualized by coomassie staining; were excised out of the native gel, and run on 10% SDS-gel. Very few peptides were bound to the glucan (data not shown). The proteins that were bound were transferred onto a nitrocellulose membrane for performing western blotting using maize SSI antibody. There was one protein in banana, two in basella, one in carrot, two or more in maize, one or two in green beans, two in rice, none in rutabaga and two in sweet potato, and two or three in wheat, were recognized by maize SSI antibody (Figure B). In order to confirm that these proteins that were bound to the glucan in the native gel and cross-reacted with maize SSI antibody posses starch synthase activity, a renaturing gel was run (see experimental procedures for details). These gels revealed both synthetic and degradative enzyme activity (Figure C). There were two proteins in banana, two in basella, one in maize, and two in wheat that possessed synthetic activity. Degradative enzyme activity was revealed in carrot, green bean, sweet potato and wheat (C). Figure D shows mobility of starch synthase enzymes of Basella alba in native gels containing no substrates (Controls).

[0129] FIG. 18A shows SDS Gel Electrophoresis;

[0130] FIG. 18B shows Western Blot Using Maize SSI antibody;

[0131] FIG. 18C shows Gel Electrophoresis to Detect Enzyme Activities; and

[0132] FIG. 18D shows Native gel Electrophoresis of Basella leaf extracts to detect SS enzyme like activity.

[0133] FIG. 19. Shows a native gel containing 0.05% potato amylopectin and displays the differences in the mobility revealed by activity staining of maize SSI, GBSS (purified from the granules) and SSIIa enzymes.

DETAILED DESCRIPTION OF THE INVENTION

[0134] Starch is deposited in granular storage bodies in most higher plants and is composed of amylose and amylopectin. Amylose is a lightly branched glucan polymer without any specific higher order of complexity. Amylopectin is composed of glucan chains arranged in a repeating structure which is made up of a highly branched amylopectin backbone arranged with the branches primarily located in an amorphous region, followed by a highly ordered crystalline lamella region lacking in branches. This crystalline region has been represented in models as a “side chain liquid crystal”, where it's mobility state is determined by the degree of order amongst the liquid crystals. Changing the degree of order then has the effect of changing the cooking properties of the starch. In normal starches there is already known to be considerable order in these liquid crystalline lamella regions, but due to this invention this order can be increased further or even decreased. It is generally known that after degrading-away the highly branched region, the remaining glucans are found to vary in chain length quite considerably. This variation in chain length is one factor determining the degree of order in the lamella region. By increasing or reducing this variation in chain lengths, useful improvements in the properties of amylopectin inside the starch granule as well as its properties after being denatured. In another aspect of this invention is increasing the efficiency of synthesis of amylose. Thus it is possible to significantly change the properties of amylopectin and amylose with consequent changes in uses of different starches. These changes in starch properties can be characterised using various Theological instruments. A further embodiment of this invention is to increase the amount of starch formed within the developing granule as a result of a more tightly ordered array of liquid crystals. In this instance the amylopectin chains which vary in chain length are made more uniform and this has the effect in making the starch pack more densely into the same space in the liquid crystal lamellae region. This has the potential not only to change starch properties, but also to increase yield as well as to increase the density of individual starch granules. This is useful because it will increase yield and also facilitate easier isolation and purification of the new starch.

[0135] Some previously well characterized ways of altering amylose and amylopectin chain length distribution involves using mutants and/or biotechnology to alter the ratios of enzymes responsible for synthesizing starch. These enzymes include the various isoforms of starch synthases, branching enzymes and debranching enzymes. This patent envisions ways of further altering amylose and amylopectin structure by engineering changes in starch synthase proteins. In particular, specific regions of certain proteins will be linked to other regions from different proteins. This engineering is made possible by the present invention which provides the specific functions of certain domains within the starch synthase proteins. Using a combination of biochemical studies and molecular evaluation, four different regions were identified within the starch synthase class of proteins. Each domain has a different yet specific function and each function is different between the different starch synthase isoforms. First is a glucan association domain (GLASS) which is responsible for determining the chain length specificity of the enzymes and their ability to associate with starch. Second is a linker domain (LINKR) responsible for proper substrate processing and separate GLYTR and GLASS domains This domain also facilitates in setting the limits on the length of glucan chains being made. Third is a glucosyl transferase domain (GLYTR) responsible for the stepwise addition of a catalytically-activated glycosyl moiety to the non-reducing end of the amylose or amylopectin glucan chain. Fourth is the C-terminal end (CTEND),which is responsible for proper folding of the overall protein. The present invention provides, in certain embodiments, proteins, peptides and/or polypeptides which are a mix and/or match these four different domains selected from different starch synthase proteins. Since many of these proteins have been identified and cloned it is possible to envision many ways to bioengineer many different combinations of new enzymes. Such new combinations of enzymes will have significantly new properties such as increased enzyme catalytic efficiency as well as changed specificity with respect to glucan chain length. A further extension of this invention is to replace the alpha-1,4glycosyl transferase catalytic domain (GLYTR) with another glycosyl transferase domain having different properties such that the glucan addition would be in a different 3314 configuration in the amylopectin molecule. For example this enhancement could place alpha-1,3 glucans in amongst the alpha-1.4 glucans normally found in starch.

[0136] To achieve these changes in amylopectin structure and hence the properties of the starch, it is necessary to create new genes encoding these novel starch synthesizing enzymes. By bioengineering each domain from a specific target enzyme, it is possible to form a fusion protein containing each of these four domains. The domains have to placed in a specific order from N-terminus through to the C-terminal end (for example: GLASS, LINKR, GLYTR, CTEND). Next the new genes are engineered so that they are expressed in the plant. The enzymes are expressed during starch formation and have to be engineered to contain a transit peptide sequence. This will ensure correct targetting of the proteins to the amyloplast where starch is synthesised. Using biotechnological techniques well known in the art, the starch enhancement envisioned herein can be done in any organism and more particularly any organism that stores or synthesizes starch.

[0137] I. Fusion Peptides of Starch Synthases

[0138] Fusion proteins, also called “hybrid proteins” are polypeptide chains that contain of two or more proteins fused together into a single polypeptide. U.S. Pat. Nos. 5,202,247 and 5,137,819 describe hybrid proteins having polysaccharide binding domains and methods and compositions for preparation of hybrid proteins capable of binding to polysaccharide matrix. Also, U.S. Pat. No. 5,202,247 describes a hybrid protein linking a cellulase-binding region to a peptide of interest. A number of patents have outlined improvements in methods of making hybrid peptides or specific hybrid peptides targeted for specific uses. For example, U.S. Pat. No. 5,635,599 reports a circularly permuted ligand with high specificity and good binding affinity as part of the hybrid peptide. U.S. Pat. No. 5,648,244 describes a method for producing a hybrid peptide with a carrier peptide. This nucleic acid region when recognized by a restriction endonuclease creates a nonpalindromic 3-base over hang that allows the vector to be cleaved.

[0139] There are reports of vectors for engineering modification in the starch pathway of plants by use of a number of starch synthesis genes in various plants. Some of these polysaccharide enzymes bind to starch, glycogen or cellulose. The U.S. Pat. No. 5,349,123 described a vector containing DNA to form glycogen biosynthetic enzymes within plant cells to introduce changes in potato starch.

[0140] The present invention provides however fusion proteins made by combining or pairing various functional polypeptide domains of starch synthases to introduce a modification in the starch structure (Table V). In the present invention, the starch association domain of GBSS enzyme is fused with the functional or catalytic domains of other various SS enzymes with different and specific functionalities to introduce modifications to starch structure.

[0141] Preferred recombinant nucleic acid molecules of this invention comprise DNA encoding the above domains (“GLYTR” or “GLASS” Domains) from any organism and comprise gene sequences set forth in the tables hereof.

[0142] Preferred plasmids of this invention are adapted for use with specific hosts. Plasmids comprising a promoter, a plastid-targeting sequence, a nucleic acid sequence encoding the above domains and a terminator sequence are provided herein. Such plasmids are suitable for insertion of DNA sequences encoding the “GLYTR”or “GLASS”domains with a LINKR or space sequence in between for expression in selected hosts. The invention includes plasmids comprising promoters adapted for both prokaryotic and eukaryotic hosts. The said promoters may also be specifically adapted for expression in monocots or in dicots.

[0143] The said fusion polypeptide according to the present invention has five regions.

N-terminal	GLASS	LINKR	GLYTR	CTEND
ARM(transit peptide)	Peptide	Peptide	Peptide	Peptide

[0144] LINKR peptide is the region between the GLASS and GLYTR and can comprise any of the sequences listed in SEQ ID NO's 243-339.

[0145] CTEND is the C-terminal region of GBSS and similar proteins and can comprise 20 to 40 amino acid residues from the list provided in Seq ID NO.I

[0146] The DNA Construct for expressing the fusion protein domains within the host, broadly is as follows:

		Transit
		Peptide/
		And/or
		N-term
Promoter		ARM
Termi-		Coding	Coding Regions for fusion peptides
nator	Intron*	region	GLASS	LINKR*	GLYTR	CTEND*
*= optional components

[0147] As is known in the art, a promoter is a region of DNA controlling transcription. Different types of promoters will be selected for different hosts. Lac and T7 promoters work well in prokaryotes, the 35S CaMV promoter works well in dicots. And the polyubiquitin promoter works well in many monocots. Other suitable promoters include maize 10 kDa Zein promoter, GBSS promoter, ST1 promoter, TR1 promoter, napin promoter etc. Any number of different promoters are known to the art can be used within the scope of this invention. It can be constitutive, inducible, tissue specific and may be homologous or heterologous to the said plant.

[0148] Also, as is known to the art, an intron is a nucleotide sequence in a gene that does not code for the gene product. One component of an intron that often increases expression in monocots is the Adh1 intron. This component of the construct is optional.

[0149] The transit peptide-coding region is a nucleotide sequence that encodes for the translocation of the protein into organelles such as plastids and mitochondria. It is preferred to choose a transit peptide that is recognized and compatible with the host in which the transit peptide is employed. In this invention the plastid of choice is the amyloplast. An example is Ferredoxin transit peptide that worked well for us in the past.

[0150] It is preferred that the hybrid polypeptide be located within the amyloplast in cells such as plant cells that synthesize and store starch in amyloplasts. If the host is a bacterial or other cell that does not contain an amyloplast, there need not be a transit peptide-coding region.

[0151] A terminator is a DNA sequence that terminates the transcription. The fusion polypeptides may also include post-translational modifications known to the art such as glycosylaiton, acylation, and other modifications not interfering with the desired activity of the polypeptide.

[0152] Brief Description of the Procedure for Developing Fusion Polypeptide

[0153] A genetic construct encoding a fusion of the invention may be obtained by “combining” the nucleotide sequences encoding at least one desired protein or polypeptide with at least one nucleotide sequence that codes for “GLYTR” or “GLASS” domains optionally with or via one or more sequences that encode a “LINKR” and “CTEND” sequences as described above, in such a way that expression of the combined sequences in the desired plant or any other organism leads to the formation of the fusion.

[0154] Genes can be cut and changed by ligation, mutation agents, digestion, restriction and other such procedures for example, as outlined in Sambrook et al., “Molecular Cloning: A Laboratory Manuel”, 2nd edition, Vols1-3, Cold Spring Harbor Laboratory(1989).

[0155] In addition, the sequences encoding for the “GLYTR” or “GLASS” domains ,“LINKR” and “CTEND” regions can be provided synthetically using known DNA synthesis techniques or isolated from a suitable biological source.

[0156] In addition to the elements mentioned above, the genetic construct encoding the fusion proteins of the invention may further contain all other elements known per se for nucleic acid sequences or genetic constructs, such as other control elements, terminators, translation or transcription enhancers, integration factors, signal sequences, and selection markers etc., that are preferably suited for use in the transformation of the host plant. The sequences that encode these further elements of the construct may be isolated from a biological source or synthesized synthetically. The one or more nucleotide sequences encoding these elements of the construct again can be combined with the nucleotide sequence encoding the fusion in a manner described in in Sambrook et al., “Molecular Cloning: A Laboratory Manuel”, 2nd edition, Vols.1-3, Cold Spring Harbor Laboratory(1989). The genetic construct encoding the fusion proteins may also include post-translational modifications known to the art such as glycosylation, acylation, and other modifications not interfering with the desired activity of the polypeptide.

[0157] Construct Development

[0158] According to one preferred embodiment, the genetic construct encoding the fusion is preferably in a form suitable for transformation of a plant, such as a vector or plasmid. The recombinant nucleic acid sequence of this invention is inserted into a convenient cloning vector or plasmid. For the present invention the preferred host is a starch granule-producing organism. However, bacterial hosts can be employed. In bacterial host, transcriptional regulatory promoters include lac, TAC, trp and the like. Additionally, DNA coding for transit peptide most likely would not be used and a secretory leader that is upstream from the structural gene may be used to get the polypeptide into the medium. Alternatively, the product is retained in the host and the host is lysed and the product isolated and purified by starch extraction methods or by binding the material to a starch like matrix such as amylose, or amylopectin, glycogen or the like to extract the product.

[0159] The cloning vector may contain coding sequences for a transit peptide to direct the plasmid into the correct location. Examples of transit peptide sequences are shown in

[0160] Coding sequences for other transit peptides can be used. Transit peptides naturally occurring in the host to be used are preferred.

[0161] Attached to the transit peptide coding sequence is the DNA sequence encoding the N-terminal end of the fusion protein domain. The direction of the sequence encoding the fusion protein is varied depending on whether sense or antisense transcription is desired. DNA constructs of this invention specifically described herein have the sequence encoding the “GLASS” domain at the N-terminus end but the “GLYTR” domain can also be at the N-terminus end and the “GLASS” sequence following. The same procedure applies to inserting “LINKR” and “CTEND” regions if needed. At the end of theDNA construct is the terminator sequence. Such sequences are well known in the art.

[0162] The cloning vector is transformed into a host. Introduction of the cloning vector, preferably a plasmid, into the host can be done by a number of transformation techniques known to the art. These techniques may vary by host but they include microparticle bombardment, micro-injection, Agrobacterium transformation, electroporation, and the like. If the host is a plant, the cells can be regenerated to form plants. Methods of regeneration of plants is known in the art. Once the host is transformed and the proteins expressed therein, the presence of the DNA encoding the fusion protein in the host is confirmed. Transcript levels can be measured and the presence of fusion proteins may b econfirmed by Western blotting or ELISA or as a result of change in the Theological properties of starch.

[0163] With regard to starch synthase fusion proteins, WO 98/14601 provides similar methods to generate naturally occuring starch that has been modified to comprise the payload peptide and not associated with bringing any structural changes to the starch or glucan chain lengths. The present invention is based, in part, on the further discoveries regarding SS enzymes and their constituent domains (detailed information provided herein below) and further evidence for the mechanism of protein entrapment in the starch granules. The present invention provides therefore methods for making and using ‘starch synthase fusion proteins’ and producing transgenic plants capable of producing “structurally modified starch” or starch granules as described herein below. Such “structurally modified starch” of the present invention differs from naturally occurring starch in the plant by at least one property thereof, such as crystallinity, branching degree, glucan composition and glucan chain length.

[0164] With regard to sequences of the starch association domain, WO 98/14601 described the idea of a hybrid polypeptide comprising: (a) a starch binding domain, and (b) payload polypeptide fused to said starch binding domain. Said starch binding domain is referred as “starch-encapsulating domain”. It may be any starch binding domain known per se, for instance derived from soluble starch synthase I, IIa, IIb, Du1, GBSS, branching enzyme I, IIa, IIb, and/or glucoamylase polypeptides. The present invention provides, in at least one embodiment, a polypeptide sequence of GBSS that will enable fusion proteins to be entrapped in the granular matrix.

[0165] With regard to structural modification of starch, WO 98/14601 provides a “peptide-modified starch” for nutritious feed. WO 98/14601 provides for encapsulation of desired amino acids or peptides within starch and specifically within starch granule to increase the plants capacity to produce a specific protein, peptide or provide an improved aminoacid balance. WO 98/14601 defined modified starch as the naturally occurring starch that has been modified to contain a payload polypeptide. Payload polypeptides are described therein as hormones or other medicaments, e.g. insulin in a starch encapsulating form to resist degradation by stomach acids for producing the payload polypeptides in easily purified form or to enhance the amino acid content of particular amino acids in the starch to provide grain feeds enriched in certain amino acids. The present invention provides, in some embodiments, methods of making and using “structure-modified starch”, such as may be used in various industrial applications.

[0166] WO 98/14601, provides for a payload polypeptide which is not endogenous to the starch encapsulating region whose expression is desired in association with this region to express a starch containing the payload polypeptide. Specific examples of payload polypeptides described therein are hormones, eg. Insulin, a growth factor like somatotropin, calcitonin, beta endorphin, urogastrone, beta globin, myoglobin, human growth hormone, angiotensin, proline, proteases, beta-galoctosidase, and cellulase, antibody, an enzyme, immunoglobulin, or dye, prolactin, and serum albumins etc.

[0167] The present invention provides polypeptides, in at least one embodiment, which are capable of interacting with starch or starch granules and show an affinity and/or enzymatic activity with starch, such that the polypeptides of the present invention may be associated with modifying glucan chain lengths of amylopectin. The present invention further provides for fusion proteins containing one starch association domain and one catalytic domain of SS enzyme that alters, converts and modifies starch structure. The present invention provides a means and methods therefore to modifying starch structure.

[0168] II. Domains of the Enzymes Involved in Starch Metabolism and their Potential Uses

[0169] Enzymes, particularly from microorganisms, are known that interact with starch. These enzymes generally contain one or more catalytic domain, and one or more regions that can bind to starch or starch granules and referred to as “starch binding domains” or “starch binding regions”. Starch association-domains for starch synthesis enzymes in higher plants however are not known or described in the literature.

[0170] Svensson et al., Biochem. J. (1989), 264, 309-311, described the sequence homology between putative starch binding domains from α-amylase from Streptomyces limosus, β-amylase from Clostridium thermosulfurogenes, glucoamylase from A.niger, maltogenic α-amylase from Bacillus stearothermophilus, malto-tetraose forming amylase from Pseudomonas slutzeri, CGTase from Bacillus, CGTase from Klebsiella pneumoniae and glucoamylase from Rhizopus oryzae. Various starch-binding domains were also compared by Janecek& Sivcek, 1999 (FEBS letters 456, 119-125). It has been suggested that some conserved tryptophan residues and the amino acids directly adjacent may play an important role in starch binding (vide Goto et al., Appl. Environ.Microbiol.,1994, p3926-3930, Chen et al., Protein engineering, 1995, Vol 8:1049-1055, Williamson et al., Biochemistry, 1997, 36:7535-7539). Chen et al., 1991, Gene 99, 121-126, Biotechnol. Prog. 1991, 7: 225-229 described a fusion of B3-galactosidase and the starch-binding domain from an Aspergillus glucoamylase, plasmids encoding such a fusion, and expression in E. coli. The starch-binding region is used to increase the affinity of β-galactosidase for starch granules, in particular as an affinity tail for recovery or enzymatic immobilization using native starch granules as an absorbant.

[0171] The use of starch binding domain fusions in oral care compositions that contain such fusions is described in the patent WO 98/16190. The fusions were prepared by expression of an appropriate expression vector in a suitable microorganism. WO 99/15636 describes starch-binding domains, and in particular the “D” and “E-domains” of the maltogenic amylase from Bacillus StearothermophilusC599, and expression thereof in a Bacillus host cell. This patent also described fusions of starch binding domain and a reporter gene such as GFP to monitor the expression of the starch binding domains in the Bacillus host. This patent only describes expression in Bacillus host and does not describe fusion of starch binding domain and an enzyme that can interact with starch or starch granule.

[0172] Dalmia et al., Biotechnology and Bioengineering, 1995, 47:575-584 described fusions of β-galoctosidase and the starch binding domains of glucoamylase I of Aspergillus awamori and of cyclodextrin glucanotransferase (domain E of CGTase) from Bacillus macerans, respectively, plasmids encoding such fusions, and expression of said fusions in E. coli. The fusion proteins thus obtained are said to bind specifically to potato starch, corn-starch, and cross-linked amylose. As a possible application, the use of the starch binding domains as an “affinity tag” is suggested. Similarly, Dalmia et al., 1994, Enzyme Microb.Technol, vol 1. describe fusions containing a starch binding domain from Aspergillus niger glucoamylase, which is again used as an affinity tail to facilitate the one step purification of the target β-galactosidase. The use of cellulose binding domains as an affinity tag for protein purification (i.e. a fusion of a cellulose binding domain from cellulase and a-galactosidase) has also been described in the art by vide Ong et al., 1989, Trends in Biotechnology, 7:239-243.

[0173] All the above references describe fusions of a starch binding domain and an enzyme, said fusions are only expressed in micro-organisms such as E. coli. The starch binding domain is included only as a “tail” or tag in order to facilitate the isolation and purification of the desired enzyme activity from the bacterial culture medium.

[0174] The use of fusion proteins in plants in situ, in particular entrapped in the starch granules has not been previously described in the literature. WO 98/14601 describes entrappment of a “payload polypeptide” in order to make a nutritionally enriched starch. The expression in plants of fusions containing enzyme doamins that can alter the length of glucan chains in amylopectin, and there by produce modified starch had not been described or suggested however in the literature.

[0175] The present application provides a means of altering starch structure and deposition in plants by using novel starch synthesizing enzymes whose catalytic properties have been found to be substantially different from known enzymes. Starches produced in plants expressing these enzymes, which are also provided by the present invention, are substantially new and novel.

[0176] The genetic constructs described in this patent may be of plant, fungal, bacterial or animal origin, and are generally incorporated into the plant genome by sexual crossing or by transformation. The enzyme gene products may be an additional copy of a wild-type gene or may encode a modified enzyme with improved properties. Incorporation of the enzyme gene construct(s) into crop plants may have varying effects depending on the amount and type of enzyme gene(s) introduced. It may also increase the plant's capacity to produce starch, in particular by altering the temperature optimum for enzyme activity, giving increased yield. It may also result in production of starch with an altered fine structure (or quality) as the exact structure depends on the novel enzyme introduced. In examples where starch structure has been altered there have generally been starch-synthesizing enzymes expressed in a wild-type background via sexual crossing. The following patent applications describe this concept in detail: PCT/GB92/01881; US application numbers 4,35,020 and 9,30,935, European publication number EPA 3,68,506 (published May 16, 1990); UK patent application number 9,218,185.8. The disclosures of these applications are hereby incorporated by reference.

[0177] III. Starch Synthases

[0178] Both prokaryotic and eukaryotic cells use polysaccharides as a storage reserve. In the prokaryotic cell the primary reserve polysaccharide is glycogen. Although glycogen is similar to the starch found in most vascular plants it exhibits different chain lengths and degrees of polymerization. In many plants, starch is used as the primary reserve polysaccharide. Starch is stored in the various tissues of the starch bearing plant. Starch is made of two components in most instances; one is amylose and the other amylopectin. Amylose is formed as essentially linear glucans and amylopectin is formed as a more highly-branched chains of glucans. Typical starch has a ratio of 25% amylose to 75% amylopectin. Starch synthases (EC 2.4.1.11) elongate starch molecules and act on both amylose and amylopectin. Starch synthase (SS) activity can be found associated both with the granule and in the stroma of the plastid. Variations in the amylose to amylopectin ratio in a plant can affect the properties of the starch. Additionally starches from different plants often have different properties. Maize starch and potato starch appear to differ due to the presence or absence of phosphate groups. Certain plants' starch properties differ because of mutations that have been introduced into the plant genome. Mutant starches are well known in maize, rice, and peas and the like.

[0179] The changes in starch branching or in the ratios of the starch components result in different starch characteristic. One characteristic of starch is the formation of starch granules that are formed particularly in leaves, roots, tubers and seeds. These granules are formed during the starch synthesis process. Certain synthases of starch, particularly granule-bound starch synthase, soluble starch synthases and branching enzymes are proteins that are “granule bound” within the starch granule when it is formed (Smith et al., 1997, Ann. Rev. Plant Physiol.Plant Mol. Biol. 48, 67-87).

[0180] Different isoforms of soluble starch synthase have been identified and cloned in pea (Denyer and Smith, 1992, Planta 186: 609-617; Dry et al., 1992, Plant Journal, 2: 193-202), potato (Edwards et al., 1995, Plant Physiol 112: 89-97; Marshall et al., 1996, Plant Cell 8: 1121-1135), wheat (Gao and Chibbar, 2000; Genome. Vol 43: 768-775), and in rice (Baba et al., 1993, Plant Physiol. 103: 565-573), while barley appears to contain multiple isoforms, some of which are associated with starch branching enzyme (Tyynela and Schulman, 1994, Physiol. Plantarum 89: 835-841).

[0181] The capacity for starch association of the bound starch synthase enzyme is well known. Various enzymes involved in starch biosynthesis are now known to have differing propensities for binding as described by Mu-Forster et al. (1996, Plant Phys. 111: 821-829). Granule-bound starch synthase (GBSS) activity is strongly correlated with the product of the waxy gene (Shure et al., 1983, Cell 35: 225-233). The synthesis of amylose in a number of species such as maize, rice and potato has been shown to depend on the expression of this gene (Tsai, 1974, Biochem Gen 11: 83-96; Hovenkamp-Hermelink et al., 1987, Theor. Appl. Gen. 75: 217-221). Visser et al. described the molecular cloning and partial characterization of the gene for granule-bound starch synthase from potato (1989, Plant Sci. 64(2):185-192).

[0182] In starch producing plants starch is usually synthesized in the form of starch granules. A number of enzymes in the plant especially the ones involved in the starch synthesis and degradation interact in vivo with these granules. These include the enzymes such as starch synthases, branching enzymes and debranching enzymes, and amylases etc. for which reference is made to Mu, C., Ham, C., Ko, Y. T., Singletary, G. W., Keeling, P. L. and Wasserman, B. P. Plant J., 1994, 6:151-159, Smith, A. M., Denyer, K., and Martin, C. Annu.Rev.Plant Physiol. Mol. Biol., 1997, 48:67-87 and Martin C., and Smith A.M. The Plant Cell, 1995, 7:971-985. However, compared to any other enzyme, granule bound starch synthase (GBSS) is the most abundant protein entrapped in the starch granules with highest affinity to amylopectin.

[0183] The present invention also classifies maize α-1,4 glucan transfereases or starch synthases based on their specificities to process various lengths of α-1,4 glucan chains in the amylopectin cluster. For example, according to the present invention, SS enzymes are defined in 4 classes. ‘Class I’ enzymes that include maize SSI and like enzymes, and preferentially elongate α-1,4 glucan chains to synthesize shorter A and B1 chains; ‘Class II’ enzymes that include maize SSIIa and SSIIb and like enzymes, and preferentially add a glucose unit(s) to α-1,4 glucan chains to synthesize longer A and B1 chains and intermediate B2 or B3 chains; ‘Class III’ enzymes that include maize SSIII and preferentially add a glucose unit(s) to α-1,4 glucan chains to synthesize longer A, B1, B2 and B3 chains as well as longer B3 or C chains of amylopectin. “Class IV” enzymes include GBSS and preferentially add a glucose unit(s) to α-1,4 glucan chains to synthesize longer B3 or C chains of amylopectin as well as amylose. In maize or any other crop when transformed to express or overexpress any one specific class of starch synthases described above will result in an increased number of glucan chains in that specific class. This patent application relates to modification of starch structure by introduction/entrapment of polypeptide domains of other soluble starch synthases (SSS) in addition to GBSS (in the form of GBSS+SSS enzyme fusion proteins) within the starch granule matrix. Therefore, the present invention provides new starch synthases other than GBSS or SSI within the starch granule matrix. These enzymes contain starch association domain of either GBSS or SSI as described above and herein which provides starch association properties similar to wild type GBSS or SSI while retaining the α-1,4 glucan transferase activity (catalytic activity) of either GBSS or soluble starch synthases such as GBSS, SSI, SSIIa, SSIIb, and SSIII and the like. Starches produced in plants expressing these enzymes are substantially new and novel.

[0184] The use of cDNA clones of animal and bacterial glycogen synthases are described in PCT/GB92/01881. The use of cDNA clones of plant soluble starch synthases has been reported. For example, the amino acid sequences of pea soluble starch synthase isoforms I, and II were published byDry et al. (1992, Plant Journal, 2:193-202) and SSIII (Gao et al., 1998). The amino acid sequence for rice soluble starch synthase was described by Baba et al.,(1993, Plant Physiology). This last sequence (rice SS) incorrectly cites the N-terminal sequence and hence is misleading. Presumably this is because of some extraction error involving a protease-degradation or other inherent instability in the extracted enzyme. The correct N-terminal sequence (starting with AELSR) is present in what they refer to as the transit peptide sequence of the rice SS.

[0185] Branching enzyme [α1,4Dglucan: α 1,4Dglucan 6D(α1,4Dglucano) transferase (E.C. 2.4.1.18)], some times called Q-enzyme, converts amylose to amylopectin. A segment of a α 1,4Dglucan chain is transferred to a primary hydroxyl group in a similar glucan chain. Bacterial branching enzyme genes and plant sequences have been reported (rice endosperm: Nakamura et al., 1992, Physiologia Plantarum, 84:329-335 and Nakamura and Yamanouchi, 1992, Plant Physiol., 99:1265-1266; pea: Smith, 1988, Planta, 175:270-279 and Bhattacharyya et al., 1990, J. Cell Biochem., Suppl. 13D:331; maize endosperm: Singh and Preiss, 1985, Plant Physiology, 79:34-40; VosScherperkeuter et al., 1989, Plant Physiology, 90:75-84; potato: Kossmann et al., 1991, Mol. Gen. Genet., 230(12):39-44; cassaya: Salehuzzaman and Visser, 1992, Plant Mol Biol, 20:809-819). The sequence of maize branching enzyme I was investigated by Baba et al., 1991, BBRC, 181:87-94. Starch branching enzyme II from maize endosperm was investigated by Fisher et al.(1993, Plant Physiol., 102:1045-1046). The use of cDNA clones of plant, bacterial and animal branching enzymes have been reported. The nucleotide and amino acid sequences for bacterial branching enzymes (BE) are known from the literature. For example, Kiel et al. cloned the branching enzyme gene glgB from Cyanobacterium synechococcussp PCC7942 (1989, Gene (Amst), 78(1): 918) and glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expressed in Escherichia coli and Bacillus subtilis Kiel J A. Boels J M. Beldman G. Venema G. (1991, Molecular & General Genetics. 230(1-2):136-44). The genes glc3 and gha1 of S. cerevisiae are allelic and encode the glycogen branching enzyme (Rowen et al., 1992, Mol. Cell Biol., 12(1): 22-29). Matsumomoto et al. investigated glycogen branching enzyme from Neurospora crassa (1990, J. Biochem., 107:118-122). The GenBank/EMBL database also contains sequences for the E. coli glgB gene encoding branching enzyme.

[0186] A common characteristic of SS clones is the presence of a KXGGLGDV consensus sequence that is believed to be the ADP-Glc binding site of the enzyme (Furukawa et al., 1990, J Biol Chem 265: 2086-2090; Furukawa et al., 1993, J. Biol. Chem. 268: 23837-23842). See below for example, the SS enzymes from various organisms

[0187] Granule Bound Starch Synthases (GBSS)

[0188] Accession Numbers:

[0189] gi|2833387|sp|Q43654|;gi|2833377|sp|;gi|2833381|sp|Q42857|; gi|2833388|sp|Q43784|gi|2829792|sp|P93568|; gi|2833383|sp|Q43092|; gi|136757|sp|P04713|; gi|136755|sp|P09842|; gi|267196|sp|Q00775|gi|2833382|sp|Q42968|; gi|2833384|sp|Q43093|; gi|6136121|sp|082627|; gi|2833385|sp|Q43134|;gi|136765|sp|P27736|; gi|2833390|sp|Q438471; gi|136758|sp|P19395|; gi|2833389|sp|Q43846|;gi|2842612|sp|Q59001|; gi|2811062|sp|O08328|; gi|729578|sp|P39125|; gi|2829618|sp|P74521|; gi|1169908|sp|P08323|; gi|121295|sp|P05416|; gi|729577|sp|P39670|; gi|1169909|sp|P45179|;

[0190] Soluble Starch Synthases (SSS)

[0191] Accession Numbers

[0192] gi|2129898|pir||S61505;gi|7489826|pir||T01265;gi|9502143|gb|AAF87999.1|AF258608—1 gi|2833390|sp|Q43847|;gi|7489274|pir||T07663;gi|4582789|emb|CAB40374.1|;gi|8573760|g b|AAC17969.2|;gi|8953573|emb|CAB96627.1|;gi|2833384|sp|Q43093 |;gi|12019656|gb|AAD 45815.2|gi|2833387|sp|Q43654|;gi|6467503|gb|AAF13168.1|AF173900—1;gi|7433871 |pir||S7 4473gi|7188796|gb|AAF37876.1|AF234163—1;gi|8708896|gb|AAC17970.2|;gi|8953571 |emb |CAB96626.1|;gi|2833389|sp|Q43846|;gi|10177090|dbj|BAB10396.1|;gi|9502145|gb|AAF88 000.1|;gi|7489695|pir||T06798;gi|5825480|gb|AAD53263.1|AF155217;gi|9369336|emb|CAB 99210.1|gi|8901183|gb|AAC17971.2|;gi|5880466|gb|AAD54661.1|;gi|2829792|sp|P93568|; gi|7488349|pir||T04926;gi|3192881|gb|AAC19119.1|;gi|7529653|emb|CAB86618.1|;gi|6103 327|gb|AAF03557.1|;gi|7489712|pir||T01414;gi|7489711|pir||T01209;gi|9369334|emb|CAB9 9209.1|gi|5295947|dbj|BAA81848.1|; gi|549232|dbj|BAA07396.1|;gi|7489710|pir||T01208;gi|2833377|sp|Q40739|; gi|729578|sp|P39125|; gi|3688125|emb|CAA06959.1|;gi|9587348|gb|AAF89274.1|; gi|9587352|gb|AAF89276.1|AF286003—1|;gi|9587319|gb|AAF89261.1|AF285986—1|;gi|9587 329|gb|AAF89266.1|AF285991—1;gi|9587337|gb|AAF89270.1|AF285995—1;gi|9587313|gb|AAF89258.1|AF285983—1|gi|9587317|gb|AAF89260.1|AF285985—1;gi|9587311|gb|AAF892 57.1|AF285982—1

[0193] gi|9587295|gb|AAF89249.1|AF285974—1;gi|9587307|gb|AAF89255.1|AF285980—1;gi|9587 321|gb|AAF89262.1|AF285987—1;gi|9587297|gb|AAF89250.1|AF285975—1;gi|958730|gb|AAF89252.1|AF285977;1,gi|9587339|gb|AAF89271.1|AF285996—1;gi|958733|gb|AAF892 67.1|AF285992—1

[0194] gi|9587305|gb|AAF89254.1|AF285979—1; gi|9587335|gb|AAF89269.1|AF285994—1; gi|958 7341|b|AAF89272.1|AF285997—1; |gi|9587343|gb|AAF89273.1|AF285998—1; gi|9587325|g b|AAF89264.1|AF285989—1; gi|9587293|gb|AAF89248.1|AF285973—1; gi|95873231 |gb|AAF 89263.1|AF285988—1gi|9587333|gb|AAF89268.1|AF285993—1; gi|9587299|gb|AAF89251.1|AF285976—1; gi|9587327|gb|AAF89265.1AF285990—1; gi|9587309|gb|AAF89256.1|AF28 5981—1; gi|9587303|gb|AAF89253.1|AF285978—1

[0195] Hybrid proteins or fusion proteins are polypeptide or peptide chains that contain two or more proteins or peptides fused together into a single polypeptide or peptide. Any of the starch synthase protein domains from the above listed or unlisted may be recombined as an embodiment of the present invention so as to control the interaction between SS and its substrates amylose or amylopectin. Such a recombination will allow to control the glucan chain lengths synthesized in the starch granule and therefore, control the useful properties of the starch.

[0196] IV. Glucan Association and Chain Length Specificity Characteristics of SS Enzymes:

[0197] Glucan-affinity gel electrophoresisin was used, and is described herein, as a tool to discover the precision and mechanism of interaction between the starch synthase enzymes, SSI, and GBSS, and their glucan-substrates, with which the glucan chain-lengths are determined by various starch synthases. SSI was found to have a greatly elevated affinity for increasing chain lengths of α-1,4 glucans (FIG. 8, A, B, C). Contrarily, the activity of SSI enzyme was decreased with increase in the avg. OCL of a-1, 4 glucans (FIG. 8D). Deletion of the N-terminal arm of SSI protein did not affect any of the glucan-binding characteristics (FIG. 9). Moreover, SSI enzyme activity is proportional to the average outer chain lengths of a given glucan molecule, which explains why SSI enzyme rate of catalysis is higher using glycogen (with shorter outer chains of dp ˜6.5) than with starch (avg. OCL ˜14.5) or amylopectin (avg. OCL˜11.5) (Imparl-Radosevich et al., 1998a; and references there in). During enzyme catalysis using SSI, majority of the 14C-label was incorporated into glucan chains with average dp less than 10 (FIGS. 10A & B). In contrast, GBSS displayed least affinity to glycogen (Table 1), however, with increasing outer chain lengths of glycogen, GBSS was found to have both an elevated affinity (FIG. 11) and catalytic activity (FIG. 11). The enzyme had the highest affinity for amylopectin and it preferred longer chains (>dp 20) of this molecule for chain extension as well (FIG. 11). In order to validate these results using SSI or GBSS, results to SSIIa, which does not entrap in the starch during the synthesis of starch granule, were compared. Unlike GBSS or SSI enzymes, SSIIa did not have any preference either for binding or for catalytic activity (FIGS. 12 and 13). SSIIa did not have any increased glucan binding with increase in the outer chain lengths. The activity of SSIa and Du 1 did not increase or decrease by altering the average outer chain lengths of glucan (FIG. 13). SSIIb did not prefer longer chained glycogen, but unlike SSI, the activity did not sharply drop after average outer chain length of dp 9. However, this drop occurred at average outer dp of 14 (FIG. 13). These contrasting results among the different enzymes that are exemplified herein indicate that the starch synthase enzymes ability to associate with the carbohydrate chains enable the enzyme to be entrapped inside the starch granule. Moreover, each enzyme has it's own specificity for length of the glucan chains. These findings provide a basis for explaining why GBSS and SSI are more strongly associated with the starch granules whereas SSIIa and SSIIb are poorly or not associated with the granule.

[0198] V. GLucan ASSociation Domain (GLASS Domain) of GBSS

[0199] Using GBSS enzyme, it has been demonstrated that the glucan-binding domain is not discrete at N- or C-terminus, but may be located close to the amino acid number 103 of the protein. The smallest peptide that has affinity for glucan was found to be about 18 kDa. The C-terminal region of GBSS (˜20 amino acids long) proteins from a wide range of species is conserved, and is hydrophilic and carries a net negative charge. This C-terminal extension is absent from other starch synthase isoforms and bacterial glycogen synthases. Edwards et al. (1999) have shown that this C-terminal region of the enzyme in potato confers most of the specific properties of this isoform except its processive elongation of glucan chains. This C-terminus of maize GBSS has been shown herein not be involved in binding of the enzyme to amylopectin molecule. Sequence comparison of starch synthases from different plant species reveal that in the region of the protein following the amino acid residue number 103 is highly conserved especially for GBSS enzymes as compared to soluble starch synthases. Within this 18 kDa starch associating domain, amino acid sequence “PV(L)AGT” starting at residue number 107 is highly conserved among pea, potato and in maize GBSS. There is also another sequence starting at residue number 155, “NDWHT” which is highly conserved among all known SS enzymes in maize. Therefore, one or more of these conserved regions may confer glucan-binding properties to GBSS enzyme. Overall, it is clear that the starch-affinity domain of SS enzymes is structurally different from the starch-binding domain of the degradative enzymes and unlike these enzymes, is not a single discrete domain.

[0200] The present invention provides a glucan or starch association domain of a starch synthase, such as a granule bound starch synthase peptide or soluble starch synthase which is, in one embodiment, about 18 kDa molecular weight under reducing conditions. The starch association domain of the present invention is preferably a peptide or polypeptide fragment of granule bound starch synthase (GBSS), which has an N-terminal end which is within, at most, 50 amino acids of the amino acid corresponding to about amino acid 103 of maize GBSS enzyme. Preferably, the starch association domain of the present invention has an N-terminal end which is within, at most, 50 amino acids of the amino acid corresponding to amino acid 103 of maize GBSS and extends, at most, approximately a further 200 amino acids along toward the C-terminus of GBSS enzyme. Alternatively, the association domain of the present invention has an N-terminus as described above and a C-terminus which is within, at most, 52 amino acids of the amino acid corresponding to amino acid 148 of maize GBSS. Alternatively, the association domain of the present invention is a peptide or polypeptide of GBSS corresponding to an amino acid sequence spanning amino acid positions 103±50 amino acids to about amino acid position 251±50 amino acids of the maize GBSS enzyme. Alternatively the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 40 amino acids from the amino acids corresponding to the amino acid positions 103 and 251, respectively, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 30 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectively, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 20 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectively, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 10 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectively, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 5 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectively, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 4 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectively, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to an amino acid position which are, independently, plus or minus 3 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectfully, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 2 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectively, of maize GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 1 amino acids from the amino acids corresponding to amino acid positions 103 and 251, respectively, of maize GBSS enzyme.

[0201] VI. Determination of the GLYcosyl TRansferase Domain (GLYTR Domain) of SS Enzymes

[0202] Using glucan affinity gel electrophoresis, and using SSI enzyme, the other smallest peptide that has affinity for glucan was found to be about 21 kDa. Within this 21 kDa starch associating domain, and with amino acid sequence starting the residue number 387 and with the following sequence LGLPIRPDVPLIGFIGRLD is highly conserved among all the starch synthases, and especially SSI, SSIIa, SSIIb and GBSS enzymes in maize. And, this region within or very close to the glycosyl transferase group I domain. Hence, it is highly likely that this region is involved in interaction with the glucan during the process of starch synthesis and glucan chain elongation. This is likely to be true for other SS enzymes as well due to their high sequence homology in this region. This observation also indicates that the glucosyl transferase function of SS enzymes invoves association or binding with the glucan polymer.

[0203] Therefore, the present invention provides a glycosyl transferase domain (Domain B) of a starch synthase that has affinity to glucan polymer, such as a soluble starch synthase I domain that is, in one embodiment, about 21 kDa molecular weight under reducing conditions. The glycosyl transferase domain (Domain B) of the present invention is preferably a peptide or polypeptide fragment of any starch synthase (SS), which has an N-terminal end which is within, at most, 50 amino acids of the amino acid corresponding to about amino acid 380 of maize SSI, SSIIa, SSIIb and GBSS enzymes and amino acid 1470 of maize SSIII or Du1 enzyme. Preferably, the glycosyl transferase domain of the present invention has an N-terminal end which is within 380 amino acids of maize SSI, SSIIa, SSIIb and GBSS enzymes and 1470 aminoacids of maize SSIII or Du1 enzyme, at most, 50 amino acids of the and extends, at most, approximately a further 200 amino acids along toward the C-terminus of each one of these enzymes. Alternatively, the association domain (Domain B) of the present invention has an N-terminus as described above and a C-terminus which is within, at most, 52 amino acids of the amino acid corresponding to amino acid 380 of maize SSI, SSIIa, SSIIb, and GBSS and amino acid 1470 of maize SSIII or Du1 enzyme. Alternatively, the glycosyl transferse domain (Domain B) of the present invention is a peptide or polypeptide of either SSI, SSIIa, SSIIb or GBSS corresponding to an amino acid sequence spanning amino acid positions 380±50 amino acids to about amino acid position 580±50 amino acids of the maize SS enzymes. Alternatively the N- and C-termini of the Glycosyl transferase domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 40 amino acids from the amino acids corresponding to the amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb, and GBSS enzyme; alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 30 amino acids from the amino acids corresponding to amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb and/or GBSS enzyme; and amino acid position 1470 and 1670, respectively of maize SSIII (Du-1); alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 20 amino acids from the amino acids corresponding to amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb and/or GBSS enzyme; and amino acid position 1470 and 1670, respectively of maize SSIII (Du-1); alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 10 amino acids from the amino acids corresponding to amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb and/or GBSS enzyme; and amino acid position 1470 and 1670, respectively of maize SSIII (Du-1); alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 5 amino acids from the amino acids corresponding to amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb and/or GBSS enzyme; and amino acid position 1470 and 1670, respectively of maize SSIII (Du-1); alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 4 amino acids from the amino acids corresponding to amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb and/or GBSS enzyme; and amino acid position 1470 and 1670, respectively of maize SSIII (Du-1); alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to an amino acid position which are, independently, plus or minus 3 amino acids from the amino acids corresponding to amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb and/or GBSS enzyme; and amino acid position 1470 and 1670, respectively of maize SSIII (Du-1);alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 2 amino acids from the amino acids corresponding to amino acid positions 380 and 580, respectively, of maize SSI, SSIIa, SSIIb and/or GBSS enzyme; and amino acid position 1470 and 1670, respectively of maize SSIII (Du-1); alternatively, the N- and C-termini of the association domain of the present invention may correspond to amino acid positions corresponding to amino acid positions which are, independently, plus or minus 1 amino acids from the amino acids corresponding to amino acid positions 378 and 545 for GBSS, 441 and 570 for SSI, 540-687 for SSIIa, 506 to 646 for SSIIb, and 1478 to 1600 for Du1 respectively.

[0204] The present invention preferably provides an isolated and/or purified domains, as described herein.

[0205] The above said “GLASS” and “GLYTR” domains of the present invention are alternatively defined as peptide or polypeptide amino acid sequences which are at least 80% identical or homologous with the above-described “GLASS” and “GLYTR”domains. Alternatively, the association domain of the present invention is more than 85% identical or homologous, or more than 90% identical or homologous or more than 95% identical or homologous, or more than 98% identical or homologous, or more than 99% identical or homologous, as compared with the above-described “GLASS” and “GLYTR”domains. One of ordinary skill in the art will readily be able to determine identical or homologous sequences by, for example, aligning sequences in question with the above-described sequence and calculating the percentage of amino acids which are different over the length of the above-described association domain. The identical or homologous peptide or polypeptide amino acid sequences of the present invention may also be identified, for example, by BLAST or Gapped BLAST search and/or comparisons, such as a comparison described or obtained by software obtainable from the NCBI website, such as through http://www.nih.gov, or http://www.ncbi.nlm.gov:80/BLAST/, or related site, or as described by Altschul, Stephen F. et al, 1997 “Gapped BLAST and PSI-BLAST: A new generation of protein data base search programs” Nucleic Acids Res. 25:3389-3402.

[0206] The above said “GLASS” and “GLYTR”domains of the present invention may also include conservative amino acid substitutions of the above-described association domain peptide or polypeptide. Such conservative amino acid substitutions will be recognized by one of ordinary skill in the art to include any of the following:

Amino acids Synonymous groups
Ser (S)     Ser, Thr, Gly, Asn
Arg (R)     Arg, His, Lys, Glu, Gln
Leu (L)     Leu, Ile, Met, Phe, Val, Tyr
Pro (P)     Pro, Ala, Thr, Gly
Thr (T)     Thr, Pro, Ser, Ala, Gly; His, Gln
Ala (A)     Ala, Pro, Gly, Thr
Val (V)     Val, Met, Ile, Tyr, Phe, Leu, Val
Gly (G)     Gly, Ala, Thr, Pro, Ser
Ile (I)     Ile, Met, Leu, Phe, Val, Ile, Tyr
Phe (F)     Phe, Met, Tyr, Ile, Leu, Trp, Val
Tyr (Y)     Tyr, Phe, Trp, Met, Ile, Val, Leu
Cys (C)     Cys, Ser, Thr, Met
His (H)     His, Gln, Arg, Lys, Glu, Thr
Gln (Q)     Gln, Glu, His, Lys, Asn, Thr, Arg
Asn (N)     Asn, Asp, Ser, Gln
Lys (K)     Lys, Arg, Glu, Gln, His
Asp (D)     Asp, Asn, Glu, Gln
Glu (E)     Glu, Gln, Asp, Lys, Asn, His, Arg
Met (M)     Met, Ile, Leu, Phe, Val

[0207] Alternatively, such conservative amino acid substitutions may be any of those shown in the following:

Amino acids
Ser (S) Ser, Thr, Gln, Asn
Arg (R) Arg, His, Lys
Leu (L) Leu, Ile, Met, Phe, Val, Tyr, Ala, Trp
Pro (P) Pro, Ala, Thr, Gly
Thr (T) Thr, Ser, Ala, Trp, Gln
Ala (A) Ala, Met, Ile, Leu, Phe, Val, Tyr, Trp
Val (V) Val, Met, Ile, Tyr, Phe, Leu, Val, Ala
Gly (G) Gly, Ala, Thr, Pro, Ser
Ile (I) Ile, Met, Leu, Phe, Val, Ala, Tyr, Trp
Phe (F) Phe, Met, Tyr, Ile, Leu, Trp, Val, Ala
Tyr (Y) Tyr, Phe, Trp, Met, Ile, Val, Leu, Ala
Cys (C) Cys, Ser, Thr, Met
His (H) His, Arg, Lys
Gln (Q) Gln, Gln, Asn, Thr, Ser
Asn (N) Asn, Ser, Gln, Thr
Lys (K) Lys, Arg, His
Asp (D) Asp, Glu
Glu (E) Glu, Asp
Met (M) Met, Ile, Leu, Phe, Val, Ala, Tyr, Trp
Trp (W) Trp, Met, Ile, Leu, Phe, Val, Ala, Tyr

[0208] The above said “GLASS” and “GLYTR”domain polypeptide or peptide of the present invention may be a soluble starch synthase, or granule bound starch synthase, branching enzyme, and any debranching enzyme from any cereal, such as maize, wheat, rice, sorghum or barley; a fruit-producing species such as banana, apple, tomato or pear; a root crop such as cassaya, potato, yam or turnip; an oil seed crop such as rapeseed, sunflower, oil palm, coconut, linseed or groundnut; a meal crop, such as soya, bean or pea; or any other suitable species.

[0209] The above said “GLASS” domain peptide or polypeptides of the present invention include a soluble starch synthase or GBSS of any of the above cereal, fruit-producing species, root crop, oil seed crop or meal crop, for example, or fragment thereof which preferably has an N-terminus corresponding to about amino acid 103± at most 50 amino acids of maize GBSS enzyme; more preferably corresponding to amino acid 103± at most 50 amino acids of maize GBSS enzyme, and extending, at most, approximately a further 200 amino acids along toward the C-terminus of the GBSS enzyme. In this embodiment, the glucan association domain peptide or polypeptide of the present invention may extend between any amino acid position corresponding to amino acids in the range of 53-153 of maize GBSS to any amino acid position corresponding to amino acids in the range of 98-198 of maize GBSS.

[0210] The above said “GLYTR” domain peptide or polypeptides of the present invention include a soluble starch synthase or GBSS of any of the above cereal, fruit-producing species, root crop, oil seed crop or meal crop, for example, or fragment thereof which preferably has an N-terminus corresponding to about amino acid 378±at most 50 amino acids of maize GBSS enzyme; 441±at most 50 amino acids of maize SSI enzyme; 540±at most 50 amino acids of maize SSIIa enzyme; 506+at most 50 amino acids of maize SSIIb enzyme; and 1478±at most 50 amino acids of maize Du1 enzyme and extending, at most, approximately a further 200±at most 50 amino acids along toward the C-terminus of the GBSS enzyme. In this embodiment, the glucan association domain or Domain “GLASS” peptide or polypeptide of the present invention may extend between any amino acid position corresponding to amino acids in the range of 53-153 of maize GBSS to any amino acid position corresponding to amino acids in the range of 98-198 of maize GBSS.

[0211] The present invention further provides a polypeptide or peptide as described above which is more than 85% identical or homologous, or more than 90% identical or homologous or more than 95% identical or homologous, or more than 98% identical or homologous, or 99% identical or homologous, as compared with the above-described association domain peptides or polypeptides, as described above.

[0212] The present invention further provides a glucan association domain peptide or polypeptide containing the following amino acid sequence:

[0213] SEQ. ID. No. 1

“KIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPYGEDV
VFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP
ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPY
YAEE”

[0214] The present invention also provides starch association domain peptides and polypeptides which are more than 85% identical or homologous, or more than 90% identical or homologous or more than 95% identical or homologous, or more than 98% identical or homologous, or more than 99% identical or homologous, as compared with SEQ ID No. 1 Such sequences of the present invention may be obtained or derived, for example, from any of the noted crops or plants, or from any of the sequences of the NCBI or other similar database, such as for example any of gi 136757, 2833385, 136755, 136758, 2833382, 136765, 2833388, 267196, 6136121, 2833381, 2833383, 2833377, 2833387, 2829792, 2833390, 2833384, 729578, 2811062, 1169908, 1169909, 2829618, 729577, 2833389, 1174879, 140977 or 549804 or any present in SEQ ID No. 1 wherein a sequence similar or identical or homologous to any one of SEQ ID No. 1, within the embodiments of the presently described invention may be found.

[0215] The present invention further provides starch synthase enzymes, such as starch synthase I (SSI), starch synthase II (SSIIa or SSIIb) or starch synthase III (SSIII) wherein the region in the SSI, SSIIa, SSIIb or SSIII, corresponding to amino acids 103±at most 50 amino acids, to about amino acid 148±at most 50 amino acids of GBSS has been altered, modified or made to be more homologous or identical to the sequence spanning amino acids 103±at most 50 amino acids to about amino acid 148±at most 50 amino acids of GBSS. One of ordinary skill will appreciate that the homology or identity in his region between SSI, SSIIa, SSIIb or SSIII to GBSS is about 70-80% on average. By altering or modifying or engineering SSI, SSIIa, SSIIb or SSIII enzymes, for example, according to the present invention, to contain a starch association domain more similar to GBSS, starch synthases are provided which contain the advantageous glucan association properties of GBSS while retaining, at least substantially, the catalytic properties of the starch synthases, such as SSI, SSIIa, SSIIb or SSIII. These altered or modified or engineered peptides or polypeptides will be capable of producing or containing, for example, a greater percentage of continuous glucan sequences in an amylopectin cluster than produced by wild-type starch synthases, thus providing changes in the confirmational structure of the amylopectin clusters.

[0216] The present invention therefore provides starch synthase enzymes, other than GBSS, which contain a starch association domain as described above and herein which provides starch association properties similar to wild-type GBSS while preferably retaining the α-1,4 glucan transferase (i.e., catalytic properties) of soluble starch synthases, such as SSI, SSIIa, SSIIb and SSIII.

[0217] In a further embodiment, the present invention provides soluble starch synthase enzymes, such as SSI, SSIIa, SSIIb or SSIII, containing glucan association domain polypeptides or peptides which are more than 80% to 90% identical or homologous to the GBSS glucan association domain peptide or polypeptide, or homologous or identical, as defined above and herein, in the region of the starch synthase enzyme corresponding to the GBSS glucan association domain defined above and herein.

[0218] Such further glucan association domain peptides and polypeptides may be compared with GBSS starch association domains of the invention by means known in the art and described herein. Such soluble synthase glucan association domains include, for example, the sequences of gi 2833377, gi 2833387, gi 2829792, and gi 2833389 or those shown above, which were obtained from a BLAST search. Similar, homologous or identical polypeptide or peptide amino acid sequences are provided by the present invention.

[0219] In a manner similar to that described above wherein soluble starch synthase enzymes are provided which contain a glucan association domain similar to or the same as GBSS, the present invention also provides granule bound starch synthases which contain a soluble starch synthase or soluble starch synthase-like glucan association domain, which is preferably more than 80% to 90% identical or homologous to a soluble starch synthase glucan association domain. Such an altered or modified granule bound starch synthase will preferentially provide continuous glucan sequences in an amylopectin cluster, for example, which are, on average, shorter than provided with wild-type GBSS. The modified, altered or engineered GBSS of this embodiment of the present invention provides changes in confirmational structure of amylopectin structures and, likely, amylose structure.

[0220] The modified, altered or engineered granule bound starch synthases or soluble starch synthases may include glucan association domains of different species. That is, for example, the present invention provides maize granule bound starch synthases or maize soluble starch synthases which may contain a starch association domain region or sequence which is obtained or derived from, or at least 85% (or at least 90%, or at least 95%, or at least 98%, or at least 99%) homologous or identical to a starch association domain of Basella alba, for example. In this manner, the present invention provides granule bound starch synthases and soluble starch synthases wherein the starch association domain is obtained from, derived from or at least 85% (or at least 90%, or at least 95%, or at least 98%, or at least 99%) homologous or identical to starch association domain of any cereal, such as maize, wheat, rice, sorghum or barley; a fruit-producing species, such as banana, apple, tomato or pear; a root crop such as cassaya, potato, yam or turnip; an oilseed crop such as rapeseed, sunflower, oil palm, coconut, linseed or ground nut; a meal crop, such as soya, bean or pea; or any other suitable species.

[0221] The present invention also provides starch synthases, such as soluble starch synthases and granule bound starch synthases of Basella alba (Malabar spinach) that are found to have higher affinity to glucan substrates. Moreover, the present invention provides starch synthases, such as soluble starch synthases or granule bound starch synthases of species other than Basella alba, such as those described above, which have been engineered, modified or altered to contain at least one of the catalytic peptide or polypeptide sequence or the starch association domain peptide or polypeptide sequence of Basella alba or fragments, or homologous sequence, thereof, as described above.

EXAMPLE I

Expression of Fusion Proteins (Green Flourescent Protein (GFP), Metallothionein, and Citrate Synthase) Fused to Different GBSS Domains to Demonstrate that an Amino Acid Sequence of the Present Invention is Needed for Glucan Association of Expressed Recombinant Fusion Proteins

[0222] Affinity gel electrophoresis was used to demonstrate which one of the peptide domains of GBSS would associate with the glucan present in the native gels. For details on the Native gel electrophoresis, see below and the references listed herein as well as general knowledge in the art. The results of these experiments were compared with the genetic experiments by construction of plasmids carrying fusion proteins with different lengths of GBSS protein. Maize plants were transformed with the above said constructs. Transgenic plants containing the fusion protein were tested for both the levels of expression, and mainly the glucan (starch) association of the fusion protein. It was stunning that the peptide discovered from the biochemical experiments that had the glucan association properties was found to be the same that is required for glucan association of fusion proteins in transgenic maize plants.

[0223] The following constructs were made using fusion proteins of different lengths of GBSS protein and Green fluorescent protein (GFP), synthetic metallothinein and synthetic Pig heart citrate synthase. For the procedure on making constructs see below and herein as well as general knowldge in the art.

TABLE VI
Summary of the analysis of the Fusion Proteins made with different
domains of GBSS enzyme in maize kernels
Plasmid		Expressed	Is the enzyme	Entrapped in
Identification	Gene Construct	in soluble Fraction?	Active?	the granules?
I.
pEXS 206	Transit peptide + GFP	Yes	NA	No
pEXS 208	Transit peptide + GFP + (N−) truncated (−97bp) GBSS	Yes	NA	Yes
pEXS 210	Transit peptide + GFP + full length GBSS	Yes	NA	Yes
pEXS 216	Transit peptide + (N−)truncated (−702bp)GBSS + GFP	Yes	NA	No
pEXS 218	Transit peptide + full length GBSS + GFP	Yes	NA	Yes
II.
pEXS 224	Transit peptide + (N−)truncated (−300aa)GBSS + 1 × Metallothionein	N.D	NA	No
pEXS 228	Transit peptide + (N−)truncated (−300aa)GBSS + 10 × Metallothionein	N.D	NA	No
III.
pEXS 233	Transit peptide + (N−)truncated (−482aa)GBSS + Citrate synthase	Yes	YES	No
pEXS 234	Transit peptide + (N−)truncated (−445aa)GBSS + Citrate synthase)	Yes	YES	No
pEXS 235	Transit peptide + (N−)truncated (−395aa)GBSS + Citrate synthase	Yes	YES	No

[0224]

TABLE VI A.
Summary of Protein SEQ ID Nos:
ENZYME
and
Protein
Seq.	Transit
ID.No.	peptide	GLASS	LINKR	GLYTR	CTEND
GBSS-34	35-74	75-120	121-171	172-222	223-266
SS1-267	268-283	284-335	336-386	387-437	438-461
SSIIa-462	463-474	475-526	527-577	578-628	629-676
SSIIb-677	678-681	682-732	733-783	784-834	835-882
Du-I 883	884-932	933-982	983-1033	1034-	1085-
				1084	1135
	Seq.ID.		Seq.ID.		Seq.ID.
“GLASS”	No.s	“GLYTR”	No.s	“CTEND”	No.s
GBSS:	1	GBSS:	1136	GBSS:	1146
SSI:	2	SSl:	1137	SSI:	1147
SSIIa:	3	SSIIa:	1138	SSIIa:	1148
SSIIb:	4	SSIIb:	1139	SSIIb:	1149
SSIII (Dul):	5	SSIII (Du1):	1140	SSIII (Dul):	1150

[0225]

TABLE VI b
Summary of Nucleotide Seq. ID. NO. s.
Nucleotide   sequence ID. No. s.
GBSS:        1141
SSI:         1142
SSIIa:       1143
SSIIb:       1144
SSIII (Dul): 1145

[0226]

TABLE VII
A Cartoon showing GBSS domain required for starch granular
entrapment (based on the transgenic analysis of various constructs
for entrapment of recombinant proteins)
1

EXAMPLES:

[0227]

TABLE VIII
A Cartoon Showing the results from Biochemical evidence for
the GBSS peptide required for its association to a glucan
substrate in the gel.
2

[0228] EXAMPLE I (See FIGS. 14 and 15) demonstrates the following:

[0229] 1. Both biochemical and transgenic approaches identified the same peptide domain of GBSS as the Glucan Association Domain (Herein referred to as “GLASS” domain).Without presence of this particular domain, transgenic proteins did not associate with starch present in the endosperm of maize kernels. The “GLASS” domain is separate from glycosyl transferase domain (herein referred to as “GLYTR” domain in this patent). The order in which the proteins domains were fused did not matter for protein expression or glucan association as long as the “GLASS” domain was enclosed in the fusion protein. The present invention demonstrates that fusion protein technology of starch synthase enzymes may be applied in crop plants. Active fusion proteins were recovered with significant enzyme activity. The examples provided here demonstrate that the invention may be exemplified, without limitation, in maize crop.

EXAMPLE II.

Examples of Some Possible and Functional Fusion Proteins

[0230] The “GLASS” and GLYTR” domains of various SS and GBSS enzymes were fused and the 3D-models for the recombinant fusion proteins are provided. Using Protein threading onto 3D-PSSM, all the starch synthase enzymes from maize were very well comparable with highest confidence to bacterial UTDP-N-acetylglucosamine 2-epimerase. Campbell et al.2000,Biochemistry 39:14993-15001, determined the X-ray structure of UDP-N-acetylglucosamine 2-epimerase with bound UDP and identified a high degree of structural homology to glycogen phosphorylase, and T4-phage β-glycosyl transferases. The relatioship of epimerase to these glycosyl transferses is very intriguing and a similarity to starch synthases is proposed herein as the starch synthase enzymes have the same glycosyl transferase function. It is also very intriguing that, Pfam00534 (glycosyl transferse family, group 1) domain is universal across all the starch synthases tested. FIGS. 16 and 17 show structures of UDP-N-acetylglucosamine 2-epimerase and glycogen phosphorylase created using the same database (Kelley et al., 2000, J. of Mol. Biol.299: 499-520.

[0231] EXAMPLE II (See FIGS. 16 and 17) demonstrates the following:

[0232] Fusion proteins from examples provided above displayed 3D folding very similar to the native proteins in vivo. This was accomplished when proper peptide lengths of fusions were made from “GLASS” and “GLYTR” domains. The 3D-structure of starch synthases is more closely related to UDP-N-Acetylglucosamine 2-epimerase and T4 phageB-glucosyltransferase than to glycogen phosphorylase. Also, for any fusion protein, the presence of highly conserved “Pfam 00534” domain results in similar protein folding at 3D level. Glucan transfer takes place in the catalytic or “GLYTR” domain of the present invention. One of the functions of “GLASS” domain is glucan binding as in GBSS, but also the chain length specificity is within this domain as well.

EXAMPLE III

[0233] Glucan Binding Properties of Starch Synthase Enzymes

[0234] Glucan affinity properties of various starch synthases (SS) enzymes from different plant species like banana fruit, basella leaf and carrot root, green bean pods, rice endosperm, rutabaga root, swetpotato root and wheat endosperm were examined and compared to maize endosperm SS forms. SSI enzyme from Basella alba displayed superior affinity to a given glucan (see table below) as compared to any of the maize enzymes studied so far. Therefore, the recombinant genes of SS enzymes from Basella and maize will enhance glucan-association properties of maize enzymes and thereby will result in better starch especially under adverse conditions. This transformation also results in altered amylopectin structure.

TABLE IX
A Comparison of K-values of Basella (B. alba L.) Starch
Synthase like Enzymes with Maize SSI enzyme
	Substrate	Enzyme	Temperature (4° C.)
	Amylose	Basella-Band 1	0.035a
		Basella-Band 2	0.284
		Maize SSI-2	0.35
	Amylopectin	Basella-Band 1	0.002
		Basella-Band 2	0.004
		Maize SSI-2	0.06
	Glycogen	Basella-Band 1	0.0186
		Basella-Band 2	0.112
		Maize SSI-2	1.20
	Starch	Basella-Band 1	0.006
		Basella-Band 2	0.036
		Maize SSI-2	0.09
	a= Molar concentration is based on the average outer chain length (O.C.L.) of the substrate molecule (for amylose, amylopectin, and glycogen apparent average outer chain lengths are 8-9, 11-12, and 6-7, respectively).

[0235] A screen for starch synthase enzymes from different plant species and their affinities to glucan substrates was conducted. FIG. 18. shows SDS-electrophoresis and coomassie staining of proteins from various plants, namely banana fruit, basella leaf, carrot root, maize endosperm, green bean pods, rice endosperm, rutabaga root, sweetpotato root, and wheat endosperm. The proteins were run on native gel containing 2% boiled starch. The peptides or proteins that were bound to the glucan in the well were visualized by coomassie staining. And, were excised out of the native gel, and run on 10% SDS-gel. Very few peptides got bound to the glucan (data not shown). The proteins that were bound were transferred onto a nitrocellulose membrane for performing western blotting using maize SSI antibody. There was one protein in banana, two in basella, one in carrot, two or more in maize, one or two in green beans, two in ricen none in rutabaga and two in sweet potato, and two or three in wheat, were recognized by maize SSI antibody (Figure B). In order to confirm that these proteins that were bound to the glucan in the native gel and cross reacted with maize SSI antibody posses starch synthase activity, a renaturing gel was performed (see experimental procedures for details). These gels revealed both synthetic and degradative enzyme activity (Figure C) There were two proteins in banana, two in basella, one on corn, and two in wheat that possessed synthetic activity. Degradative enzyme activity was revealed in caroot, greenbean, sweetpotato and wheat (C). Figure D shows mobility of starch synthase enzymes of Basella alba in native gels containing no substrates (Controls). Also, starch synthase enzymes within maize endosperm have different affinities to glucans (See FIG. 19).

[0236] EXAMPLE III (See FIG. 19) demonstrates the following:

[0237] Various starch synthase enzymes have different affinities to a given glucan (FIG. 19). And, hence, it is possible to manipulate the functionality of native starch synthases and provide modifications to glucan chain lengths and starch structure.

[0238] The entire contents of the following references, along with the content of any references or documents or sequence deposit or other literature referred to and/or described herein or above or with the following are incorporated herein by reference in their entirety.

[0239] WO9720936 Starch Synthase Sequences

[0240] WO9844780 Starch Synthase Hosts

[0241] WO9814601 EnCapsulation

[0242] WO9924575 Dull1 Starch Synthase III

[0243] WO92/11376; 92/14827; 98/16190; 99/15636

[0244] PCT/GB92/01881

[0245] EPA 368506

[0246] U.S. Pat. Nos.: 5,792,920; 5,824,790; 5,859,333; 6,013,861; 6,107,060; 2,061,143; 4,789,557; 4,790,997; 4,774,328; 4,770,710; 4,798,735; 4,767,849; 4,801,470; 4,789,738; 9,30935;4,35,020; 4,792,458; 5,009,911; 5,300,145; 5,202,247; 5,137,819; 5,635,599; and 5,648,244.

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SEQ. ID. No. 1
Glucan Association Domain (“GLASS) of GBSS
“K I Y G P V A G T D Y R D N Q L R F S L L C Q A A L E A P R I L S L N N N P
Y F S G P Y G E D V V F V C N D W H T G P L S C Y L K S N Y Q S H G I Y R D A
K T A F C I H N I S Y Q G R F A F S D Y P E L N L P E R F K S S F D F I D G Y
E K P V E G R K I N W M K A G I L E A D R V L T V S P Y Y A E E“
Seq ID. No. 2
Glucan Association domain (“GLASS”)domain (”GLASS”) of maize SSI
E G I A E G S I D N T V V V A S E Q D S E I V V G K E Q A R A K V T Q S I V F
V T G E A S P Y A K S G G L G D V C G S L P V A L A A R G H R V M V V M P R Y
L N G T S D K N Y A N A F Y T E K H I R I P C F G G E H E V T F F H E Y R D S
V D W V F V D H P S Y H R P G N L Y G D K F G A F G D N Q F R Y T L L C Y A A
C E A P L I L E L G G Y I Y G Q N C M F V V N D W H A S L V P V L L A A K Y R
P Y G V Y K D S R S I L V I H N L A H Q G V E P A S T Y P D L G L P P E W Y G
Seq ID. No. 3
Glucan Association domain (“GLASS”)domain (“GLASS”) of maize SSIIa
S K R R D P L Q P V G R Y G S A T G N T A R T G A A S C Q N A A L A D V E I K
S I V A A P P T S I V K F P A P G Y R M I L P S G D I A P E T V L P A P K P L
H E S P A V D G D S N G I A P P T V E P L V Q E A T W D F K K Y I G F D E P D
E A K D D S R V G A D D A G S F E H Y G D N D S G P L A G E N V M N V I V V A
A E C S P W C K T G G L G D V V G A L P K A L A R R G H R V M V V V P R Y G D
Y V E A F D M G I R K Y Y K A A G Q D L E V N Y F H A F I D G V D F V F I D A
P L F R H R Q D D I Y G G S R Q E I M K R M I L F C K V A V E V P W H V P C G
G V C Y G D G N L V F I A N D W H T A L L P V Y L K A Y Y R D H G L M Q Y T R
S V L V I H N I A H Q G R G P V D E F P Y M D L P E H Y L Q H F E L Y D P
Seq ID. No.4
GlucanAssociation domain (“GLASS”)domain (“GLASS”) of maize SSIIb
A D A A P A T D A A A S A P Y D R E D N E P G P L A G P N V M N V V V V A S E
C A P F C K T G G L G D V V G A L P K A L A R R G H R V M V V I P R Y G E Y A
E A R D L G V R R R Y K V A G Q D S E V T Y F H S Y I D G V D F V F V E A P P
F R H R H N N I Y G G E R L D I L K R M I L F C K A A V E V P W Y A P C G G T
V Y G D G N L V F I A N D W H T A L L P V Y L K A Y Y R D N G L M Q Y A R S V
L V I H N I A H Q G R G P V D D F V N F D L P E H Y I D H F K L Y D N I G
Seq ID. No. 5
GlucanAssociation domain (“GLASS”) of maize Du1
G G I Y D N R N G L D Y H I P V F G S I A K E P P M H I V H I A V E M A P I A
K V G G L G D V V T S L S R A V Q D L G H N V E V I L P K Y G C L N L S N V K
N L Q I H Q S F S W G G S E I N V W R G L V E G L C V Y F L E P Q N G M F G V
G Y V Y G R D D D R R F G F F C R S A L E F L L Q S G S S P N I I H C U D W S
S A P V A W L H K E N Y A K S S L A N A R V V F T I H N L E
SEQ. ID. No. 6
SSI
Sequence used by 3DPSSM for threading
3
4
Secondary Structure Calculated
Number of Groups    349
Number of Atoms     2708
Number of Bonds     2791
Number of H-Bonds   237
Number of Helices   20
Number of Strands   17
Number of Turns     30
SEQ. ID. No. 7
SSIIa
Sequence utilized by 3DPSSM
5
6
Secondary Structure Calculated
Number of Groups    358
Number of Atoms     2802
Number of Bonds     2885
Number of H-Bonds   246
Number of Helices   22
Number of Strands   17
Number of Turns     30
SEQ. ID. No. 8
SSIIB-
Sequence used by 3DPSSM for threading
7
8
SecondaryStructure  Calculated
Number of Groups    361
Number of Atoms     2888
Number of Bonds     3012
Number of H-Bonds   252
Number of Helices   22
Number of Strands   17
Number of Turns     31
SEQ. ID. No. 9
GBSS
GBSS Sequence used by 3D-PSSM
9
10
11
SecondaryStructure  Calculated
Number of Groups    361
Number of Atoms     2805
Number of Bonds     2877
Number of H-Bonds   252
Number of Helices   21
Number of Strands   17
Number of Turns     30
SEQ. ID. No. 10
Du I
DuI sequence used by 3D-PSSM
Note: Residue numbered as 37 (listed below) is actually amino acid residue
number 1238 in Du1 protein sequence
12
13
14
15

[0373]

TABLE III
			%
			align-
ENZYME	Seq. ID No.	Alignment with Pfam 00534 (Glycosyl transferase, Group 1 domain)	ment
GBSS	Seq. Id.	query:	378	NKEALQAEVGLPVDRNIPLVAFIGRLEEQKGPDVMAAAIPQLME-MVEDVQIVLLGTGKK	436	91.3
	No.11 (query)
	Seq. Id.	Sbjct:	1	DREEIRKKLGIKEEKKI--ILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEE	58
	No.12 (snjct)
		Query:	437	KFERMLMSAEEKFPGKVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTP	496
		Sbjct:	59	EDELKLLALKLGLEDNVIFLGFVPDEDLPELYKSADVFVLPSRYEGFGIVLLEAMACGLP	118
		Query:	497	CACASTGGLVDTIIEGKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIK 545
		Sbjct:	119	VIATDVGGIPEIVKDGETGL----------LVEPGDVEALAEAIEKLLK 157
SSI	Seq. Id.	Query:	441	LPIRPDVPLIGFIGRLDYQKGID-LIQLI--IPDLMREDVQFVMLGSGDPELEDWMRSTE	497	75
	No.13 (query)
	Seq. Id.	Sbjct:	9	LGIKEEKKIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALK	68
	No.14-(snjct)
		Query:	498	SIFKDKFRGWVGF-SVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATGGL	556
		Sbjct:	69	LGLEDNVI-FLGFVPDEDLPELYKSADVFVLPSRYEGFGIVLLEAMACGLPVIATDVGGI	127
		Query:	557	RDTVENFNPFGENG 570
		Sbjct:	128	PEIVKD----GETG 137
SSIIa	Seq. Id.	Query:	540	LEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWIA---GQDVQLVMLGTGRADLERMLQHLE	596	84.3
	No.15 (query)
	Seq. Id.	Sbjct:	9	LGIKEEKKIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALK	68
	No.16 (snjct)
Query:	597	REHPNKVRGWVGFSVPMAHRIT--AGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	654
		Sbjct:	69	LGLEDNVI-FLGF-VPDEDLPELYKSADVFVLPSRYEGFGIVLLEAMACGLPVIATDVGG	126
		Query:	655	LRDTVAPFDPFGDAGLGWTFDRAEANKLIEALR 687
		Sbjct:	127	IPEIVKDGET------GLLVEPGDVEALAEAIE 153
SSIIb	Seq. Id.	Query:	506	LQVRDDVPLIGFIGRLDHQKGVDIIADAIHWIA---GQDVQLVMLGTGRADLEDMLRRFE	562	87.2
	No.17 (query)
	Seq. Id.	Sbjct:	9	LGIKEEKKIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALK	68
	No.18 (snjct)
		Query:	563	SEHSDKVRAWVGFS----VPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAV	618
		Sbjct:	69	LGLEDNVI-FLGFVPDEDLPELYKS---ADVFVLPSRYEGFGIVLLEAMACGLPVIATDV	124
		Query:	619	GGLRDTVAP------FDPFNDTGLGWTFDRAEAN 646
		Sbjct:	125	GGIPEIVKDGETGLLVEPGDVEALAEAIEKLLKD 158
DuI	Seq. Id.	Query:	1478	PVVGIVTRLTAQKGIHLIKHAIHRTLERNGQVVLLGSAPDSRIQADFVNLANTLHGVNHG	1537	70.3
	No.19 (query)
	Seq. Id.	Sbjct:	16	KIILFVGRLLPEKGIDLLIEAFKKLKKQLNPNLKLVIVGDGEEEDELKLLALKLGLEDNV	75
	No.20 (snjct)
		Query:	1538	QVRLSLTYDEPLSHLIYAGSDFILVPSIFEPCGLTQLVAMRYGTIPIVRKTGGLFDTVFD	1597
		Sbjct:	76	IFLGFVPDEDLPEL--YKSADVFVLPSRYEGFGIVLLEAMACGLPVIATDVGGIPEIVKD	133
		Query:	1598	VDN 1600
		Sbjct:	134	GET 136

[0374]

TABLE IIIa
PIR Multiple Alignment for “GLYTR” Domain of Maize SS enzymes
Enzyme	Seq
(res. # of	Id
the start)	No	CLUSTAL W (1.8) multiple sequence alignment
SSIIa-540	21	-----------EVRDDVPLLGFIGRLDGQKGVDIIGDAMPWIAG--QDVQLVMLGTG-----RADLERMLQHLEREHPNKVRGWVGFS
		VPMAHRITAGADVLVMPSFEPCGLNQLYAMAYGTVPVVHAVGGLRDTVAPFDP-FGDAGLGWTFDRAEANKLIEALR----
SSIIb-506	22	----------LQVRDDVPLIGFIGRLDHQKGVDIIADAIHWIAG--QDVQLVMLGTG-----RADLEDMLRRFESEHSDKVRAWVGFS
		VPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAVGGLRDTAPFDP-FNDTGLGWTFDRAEAN-----------
SSI-441	23	----------LPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLMR--EDVQFVMLGSG-----DPELEDWMRSTESIKDKFRGWVGFSV
		PVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATGGLRDTVENFNP-FGENG----------------------
GBSS-378	24	NKEALQAEVGLPVDRNIPLVAFIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTG-----KKKFERMLMSAEEKFPGKVRAVVKFN
		AALANHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIEGKTGFEMGRLSVDCNVVEPADVKKVATTLC
Dul-1478	25	-----------------PVVGIVTRLTAQKGIHLIKHAIHRTLE--RNGQVVLLGSAPDS RIQADFVNLANTHGVNHGQVRLSLTYD
		*::.::**   *** .::   :       .: *.*:**:.        .:      .    .:.*  :. .
		EPLSHLIYAGSDFILVPSIFEPCGTQLVAMR YGTIPIVRKTGGLFDTVFDVDN---------------------------
		::* * **.*.: :.* ****** ** .*   ***      .*** **:   .
Footnote for all the sequence alignments and comparisons in this document:
*= identical amino acid
:.= similar amino acids
-= gaps for accommodating residues from other sequences that are not aligned.

[0375]

TABLE IIIb
PIR Multiple Alignment of SSI and SSII enzymes
		SEQ.ID.
ENZYME	CLUSTAL W (1.8) multiple sequence alignment	No
SSIIA.	MSSAAVSSSSSTFFLALASASPGGRRRARVGSSP---FHTGASLSFAFWAPPSPPRAPRD	26
SSIIB.	-MPGAISSSSSAFLLPVASSSPR-RRRGSVGAALRSYGYSGAELR-LHWARRGPPQD--G	27
SSI.	--MATPSAVGAACLLLARAAWP-----AAVG-----------DR-----ARPRRLQR---	29
	.: *: .:: :*   :: *     . **           .      *     :
SSIIA.	AALVRAEAEAGGKDAPPERSGDAARLPRARRNAVSKRRDPLQPVGRYGSATGNTARTGAA	26 (cont.d)
SSIIB.	AASVRAAAAPAGGESEEAAKSSSSSQAGAVQGSTAKAVDSASPPNPLTSAP---KQSQSA	27
SSI.	-VLRRRCVAELSREGPAPRPLPPALLA------P-----PLVP---------------------	29
	.  *  .   . :.       .:  .            .  *
SSIIA.	SCQNAALADVEIKSIVAAPPTSIVKFPAPGYRMILPSGDIAPETVLPAPKPLHESPAVDG	26 (cont.d)
SSIB.	AMQNG------------------------------TSGGSSASTAAPVSGPKADHPSAPV	27
SSI.	---------------------------------------G-FLAPPAEPTGEPASTPPPVP-	29
	*   . .. *.  *    *..
SSIIA.	DSNGIAPPTVEPLVQEATWDFKKYIGFDEPDEAKDDSRVGADDAGSFEHYG-DNDSGPLA	26 (cont.d)
SSIIB.	TKREIDASAVKPEPAGDDARPVESIGIAEPVDAKADAAPATDAAASAPYDREDNEPGPLA	27
SSI.	-DAGLGDLGLEPE------------GIAEG--SIDNTVVVASEQDSEIVVGKEQARAKVT	29
	.  :    ::*             *: *   :  ::   :.   *      ::  . ::
SSIIA.	GENVMNVIVVAAECSPWCKTGGLGDVVGALPKALARRGHRVMVVVPRYG------DYVEA	26 (cont.d)
SSIIB.	GPNVMNVVVVASECAPFCKTGGLGDVVGALPKALARRGHRVMVVIPRYG------EYAEA	27
SSI.	----QSIVFVTGEASPYAKSGGLGDVCGSLPVALAARGHRVMVVMPRYLNGTSDKNYANA	29
	.::.*:.*.:*:.*:****** *:** *** ********:***      :*.:*
SSIIA.	FDMGIRKYYKAAGQDLEVNYFHAFIDGVDFVFIDAPLFRHRQDDIYG---GSRQEIMKRM
SSIIB.	RDLGVRRRYKVAGQDSEVTYFHSYIDGVDFVFVEAPPFRHRHNNIYG---GERLDILKRM
SSI.	FYTEKHIRIPCFGGEHEVTFFHEYRDSVDWVFVDHPSY-HRPGNLYGDKFGAFGDNQFRY
	:      * : **.:** : *.**:**:: * : ** .::**   *   :   *
SSIIA.	ILFCKVAVEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDHGLMQYTRSVLV	26 (cont.d)
SSIIB.	ILFCKAAVEVPWYAPCGGTVYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMQYARSVLV	27
SSI.	TLLCYAACEAPLILELGGYIYG-QNCMFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILV	29
	*:* .* *.*     **  **  * :*:.****::*:** * * **  *: : :**:**
SSIIA.	IHNIAHQGRGPVDEFPYMDLP------EHYLQHFELYDPVG-GEHANIFAAGLKMADRV	26 (cont.d)
SSIIB.	IHNIAHQGRGPVDDFVNFDLP------EHYIDHFKLYDNIG-GDHSNVFAAGLKTADRV	27
SSI.	IHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHALDKGEAVNFLKGAVVTADRI	29
	***:****  *.. :  :.**       *  : .:   . :. *:  *.: ..: ***:
SSIIA.	VTVSRGYLWELKTVEGGWGLHDIIRSNDWKINGIVNGIDHQEWNPKVDVHLRSDGYTNYS	26 (cont.d)
SSIIB.	VTVSNGYMWELKTSEGGWGLHDIINQNDWKLQGIVNGIDMSEWNPAVDVHLHSDDYTNYT	27
SSI.	VTVSKGYSWEVTTAEGGQGLNELLSSRKSVLNGIVNGIDINDWNPATDKCIP----CHYS	29
	****.** **:.* *** **:::: ...  ::******* .:*** .*   :     :*:
SIIA.	LETLDAGKRQCKAALQRELGLEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWIAGQDVQLV	26 (cont.d)
SSIIB.	FETLDTGKRQCKAALQRQLGLQVRDDVPLIGFIGRLDHQKGVDIIADAIHWIAGQDVQLV	27
SSI.	VDDLS-GKAKCKGALQKELGLPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLMREDVQFV	29
	: *. ** :**.***::*** :* ****:******* ***:*:*   :  :  :***:*
	MLGTGRADLERMLQHLEREHPNKVRGWVGFSVPMAHRITAGADVLVMPSRFEPCGLNQLY	26 (cont.d)
SSIIA.	MLGTGRADLEDMLRRFESEHSDKVRAWVGFSVPLAHRITAGADILLMPSRFEPCGLNQLY	27
SSIIB.	MLGSGDPELEDWMRSTESIFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLY	29
SSI.	***:* .:**  ::  *  . *.*. *******::******.*:*:**************
SSIIA.	AMAYGTVPVVHAVGGLRDTVAPFDPFGDAG---LGWTFDRAEANKLIEALRHCLDTYRKY	26 (cont.d)
SSIIB.	AMAYGTVPVVHAVGGLRDTVAPFDPFNDTG---LGWTFDRAEANRMIDALSHCLTTYRNY	27
SSI.	AMQYGTVPVVHATGGLRDTVENFNPFGENGEQGTGWAFAPLTTENMFVDIANCN------	29
	** *********.*******  *:**.: *    ::.::  : :*
SSIIA.	GESWKSLQARGMSQDLSWDHAAELYEDVLVKAKYQW	26 (cont.d)
SSIIB.	KESWRACRARGMAEDLSWDHAAVLYEDVLVKAKYQW	27
SSI.	------IYIQGTQVLLGRANEARHVKRLHVGPCR--	29

[0376]

TABLE IV
Possible, but not limited to Amino acid Sequences
for Some of the Proposed Fusion Proteins
A. GBSS (61-300) + Du1 (1201-1674)
SEQ.ID. No. 30
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTS
VVSEIKMGDG YETVRFFRCY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAI
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN WMKAGILEAD RVLTVSPYYA EELISGIARG CELDNIMRLT
GITGIVNGMD VSEWDPSRDK GGIYDNRNGL DYHIPVFGSI AKEPPMHIVH IAVEMAPIAK VGGLGDVVTS
LSRAVQDLGH NVEVILPKYG CLNLSNVKNL QIHQSFSWGG SEINVWRGLV EGLCVYFLEP QNGMFGVGYV
YGRDDDRRFG FFCRSALEFL LQSGSSPNII HCHDWSSAPv AWLUKENYAK SSLANARVVF TIHNLEFGAH
HIGKAMRYCD KATTVSNTYS KEVSGHGAIV PHLGKFYGIL NGIDPDIWDP YNDNFIPVHY TCENVVEGKR
AAKRALQQKF GLQQIDVPVV GIVTRLTAQK GIHLIKHAIH RTLERNGQVV LLGSAPDSRI QADFVNLANT
LHGVNHGQVR LSLTYDEPLS HLIYAGSDFI LVPSIFEPCG LTQLVAMRYG TIPIVRKTGG LFDTVFDVDN
DKERARDRGL EPNGFSFDGA DSNGVDYALN RAISAWFDAR SWFHSLCKRV MEQDWSWNRP ALDYIELYRS
ASKL
B. GBSS (61-300) + SSI (400-622)
SEQ.ID. No. 31
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTS
VVSEIKMGDG YETVRFFECY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAL
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN KATTVSNTYS KEVSGHGAIV PHLGKFYGIL NGIDPDIWDP
NGIDINDWNP ATDKCIPCHY SVDDLSGKAK CKGALQKELG LPIRPDVPLI GFIGRLDYQK GIDLIQLIIP
DLMREDVQFV MLGSGDPELE DWMRSTESIF KDKFRGWVGF SVPVSHRITA GCDILLMPSR FEPCGLNQLY
AMQYGTVPVV HATGGLRDTV ENFNPFGENG EQGTGWAFAp LTTENMFVDI ANCNIYIQGT QVLLGRANEA
RHVKRLHVGP CR
C. GBSS (61-300) + SSIIa (481-732)
SEQ.ID. No. 32
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTs
VVSEIKMGDG YETVRFFHCY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAL
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN DIIRSNDWKI NGIVNGIDHQ EWNPKVDVHL RSDGYTNYSL
ETLDAGKRQC KAALQRELGL EVRDDVPLLG FIGRLDGQKG VDIIGDAMPW IAGQDVQLVM LGTGRADLER
MLQHLEREHP NKVRGWVGFS VPMAHRITAG ADVLVMPSRF EPCGLNQLYA MAYGTVPVVH AVGGLRDTVA
PFDPFGDAGL GWTFDRAEAN KLIEALRUCL DTYRKYGESW KSLQARGMSQ DLSWDHAAEL YEDVLVKAKY
QW
D. GBSS (61-300) + SSIIb (481-698)
SEQ.ID. No. 33
RRGGRFPSLV VCASAGMNVV FVGAEMAPWS KTGGLGDVLG GLPPAMAANG HRVMVVSPRY DQYKDAWDTS
VVSEIKMGDG YETVRFFHCY KRGVDRVFVD HPLFLERVWG KTEEKIYGPV AGTDYRDNQL RFSLLCQAAL
EAPRILSLNN NPYFSGPYGE DVVFVCNDWH TGPLSCYLKS NYQSHGIYRD AKTAFCIHNI SYQGRFAFSD
YPELNLPERF KSSFDFIDGY EKPVEGRKIN YTNYTFETLD TGKRQCKAAL QRQLGLQVPD DVPLIGFIGR
LDHQKGVDII ADAIHWIAGQ DVQLVMLGTG RADLEDMLRR FESEHSDKVR AWVGFSVPLA HRITAGADIL
LMPSRFEPCG LNQLYAMAYG TVPVVHAVGG LRDTVAPFDP FNDTGLGWTF DRAEANRMID ALSHCLTTYR
NYKESWRACR ARGMAEDLSW DHAAVLYEDV LVKAKYQW

[0377]

Amino acid sequence
of maize granule bound starch synthase (GBSS)
Accession numbers: EMBL; X03935; E276624; -;
PIR; S07314; S07314;
MAIZEDB; 15806
SEQ.ID. No. 34
1   M A A L A T S Q L V A T R A G L G V P D A S T F R R G A A Q
31  G L R G A R A S A A A D T L S M R T S A R A A P R H Q Q Q A
61  R R G G R F P S L V V C A S A G M N V V F V G A E M A P W S
91  K T G G L G D V L G G L P P A M A A N G H R V M V V S P R Y
121 D Q Y K D A W D T S V V S E I K M G D G Y E T V R F F H C Y
151 K R G V D R V F V D H P L F L E R V W G K T E E K I Y G P V
181 A G T D Y R D N Q L R F S L L C Q A A L E A P R I L S L N N
211 N P Y F S G P Y G E D V V F V C N D W H T G P L S C Y L K S
241 N Y Q S H G I Y R D A K T A F C I H N I S Y Q G R F A F S D
271 Y P E L N L P E R F K S S F D F I D G Y E K P V E G R K I N
301 W M K A G I L E A D R V L T V S P Y Y A E E L I S G I A R G
331 C E L D N I M R L T G I T G I V N G M D V S E W D P S R D K
361 Y I A V K Y D V S T A V E A K A L N K E A L Q A E V G L P V
391 D R N I P L V A F I G R L E E Q K G P D V M A A A I P Q L M
421 E M V E D V Q I V L L G T G K K K F E R M L M S A E E K F P
451 G K V R A V V K F N A A L A H H I M A G A D V L A V T S R F
481 E P C G L I Q L Q G M R Y G T P C A C A S T G G L V D T I I
511 E G K T G F H M G R L S V D C N V V E P A D V K K V A T T L
541 Q R A I K V V G T P A Y E E M V R N C M I Q D L S W K G P A
571 K N W E N V L L S L G V A G G E P G V E G E E I A P L A K E
601 N V A A P

[0378]

TABLE VII
Maize Granule bound starch synthase (GBSS)
Aligmnents with other similar proteins-Transit Peptide
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	Sequence	#
35	maize GBSS	1	MAALATSQLVATRAGLGVPD----ASTF--XXXXXXXXXXXXXXXXXDTLSMRT---S--	49
36	136757	1	MAALAT8QLVATRAGLGVPD----ASTF--RRGAAQGLRGARASAAADTLSMRT---S--	49
37	2833385	1	MSTLATSQLVATHAGLGVPD----ASMFRRGGVQGLRAAARASAAAGDALSMRT---SAC	53
38	136758	1	MSALTTSQLATSATGFGIADRSAPSSLL--RHGFQGLKPRSPAGGDATSLSVTT---S--	53
39	2833382	1	MSALTTSQLATSATGFGIADRSAPSSLL--REGFQGLKPRSPAGGDATSLSVTT---S--	53
40	297424	1	MSALTTSQLATSATGFGIADRSAPSSLL--RHGFQGLKPRSPAGGDATSLSVTT---D--	53
41	7798551	1	MSALTTSQLATSATGFGIADRSAPSSLL--RHGFQGLKPRSPAGGDATSLSVTT---S--	53
42	82478	1	MSALTTSQLATSATGFGIADRSAPSSLL--RHGFQGLKPRSPAGGDATSLSVTT---S--	53
43	297422	1	MSALTTSQLATSATGFGIADRSAPSSLL--RHGFQGLKPRSPAGGDASSLSVTT---S--	53
44	136755	1	MAALATSQLATSGTVLGVTD----------RFRRPGFQGLRPRNPADAALGMRT---I--	45
45	18652407	1	MAALATSQLATSGTVLGVTD----------RFRRPGFQGLRPRNPADAALGMRT---I--	45
46	4760582	1	MAALVTSQLATSGTVLGITD----------RFRRAGFQGVRPRSPADAPLGMRTTGAS--	48
47	11037536	1	MAALVTSQLATSGTVLGITD----------RFRRAGFQGVRPRSPADAPLGMRTTGAS--	48
48	6624287	1	MAALVTSQLATSGTVLGITD----------RFRRAGFQGVRPRNPADAALVMRT---I--	45
49	6624283	1	MAALVTSQLATSGTVLGITD----------RFRRAGFQGVRPRSPADAPLGMRTTGAS--	48
50	4760584	1	MAALVTSQLATSGTVLGITD----------RFRRAGFQGVRPRSPADAALGMRT---V--	45
51	6318538	1	MAALVTSQLATSGAVLGITD----------RFRRAGFQGVRPRSPADAPLGMRTVGAS--	48
52	6318540	1	MAALVTSQLATSATVLGITD----------RFRRAGFQGVRPRSPADAPLGMRTVGAS--	48
53	6624285	1	MAALVTSQLATSGTVLSVTD----------RFRRPGFQGLRPRNPADAALGMRT---V--	45
54	6624281	1	MAALVTSQLATSGTVLSVTD----------RFRRPGFQGLRPRNPADAALGMRTVG-A--	47
55	4760580	1	MAALVTSQLATSGTVLSVTD----------RFRRPGFQGLRPRNPADAALGMRTVG-A--	47
56	136765	1	MAALVTSQLATSGTVLSVTD----------RFRRPGFQGLRPRNPADAALGMRTVG-A--	47
57	4588609	1	MAALVTSQLATSGTVLGITD----------RFRRAGFQGVRPRSPADAALGMRT---V--	45
58	6136121	49	DKLQMRN---N--	56
59	15637079	48	DMLQLRT---S--	55
60	2833388	50	DKLQMKT---Q--	57
35 (cont.d)	GBSS	50	-XXXXXXXXXXXXXGG--RFPSLVVCA--S-AGMNVVFVGAEMAPWSKTXXXXXXXXXXP	103
36 (cont.d)	136757	50	-ARAAPRHQQQARRCG--RFPSLVVCA--S-AGMNVVFVGAEMAPWSKTGGLGDVLGGLP	103
37 (cont.d)	2833385	54	PAPRQQPAARRGGRGG--RFPSLVVCA--T-AGMNVVFVGAEMAPWSKTGGLGDVLGGLP	108
38 (cont.d)	136758	54	-APATPKQQRSVQRGSR-RFPSVVVYA--TGAGMNVVFVGAEMAPWSKTGGLGDVLGGLP	109
39 (cont d)	2833382	54	-ARATPKQQRSVQRGSR-RFPSVVVYA--TGAGNNVVFVGAEMAPWSKTGGLGDVLGGLP	109
40 (cont.d)	297424	54	-ARATPKQQRSVQRGSR-RFPSVVVYA--TGAGNNVVFVGAEMAPWSKTGGLGDVLGGLP	109
41 (cont.d)	7798551	54	-APATPKQQRSVQRGSR-RFPSVVVYA--TGAGNNVVFVGAEMAPWSKTGGLGDVLGGLP	109
42 (cont.d)	82478	54	-ARATPKQQRSVQRGSR-RFPSVVVYA--TGAGMNVVFVGAEMAPWSKTGGLGDVLGGLP	109
43 (cont.d)	297422	54	-ARATPKQQRSVQRGSR-RFPSVVVYA--TGAGMNVVFVGAEMAPWSKTGGLGDVLGGLP	109
44 (cont d)	136755	46	-GASAAPKQSRKAHRGSRRCLSVVVRA--TGSGMNLVFVGAEMAPWSKTGGLGDVLGGLP	102
45 (cont.d)	18652407	46	-GASAAPKQSRKAHRGSRRCLSVVVRA--TGSGMNLVFVGAEMAPWSKTGGLGDVLGGLP	102
46 (cont.d)	4760582	49	-AAPKQQSRKAHRGTR--RCLSMVVRATGS-AGMNLVFVGAEMAPWSKTGGLGDVLGGLP	104
47 (cont.d)	11037536	49	-AAPKQQSRKAHRGTR--RCLSMVVRATGS-AGMNLVFVGAEMAPWSKTGGLGDVLGGLP	104
48 (cont.d)	6624287	46	-GASAAPKQSRKAHRGSRRCLSMVVRATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	103
49 (cont.d)	6624283	49	-AAPKQQSRKAHRGTR--RCLSMVVRATGS-AGMNLVFVGAEMAPWSKTGGLGDVLGGLP	104
50 (cont.d)	4760584	46	-GASAAPTQSRKAHRGTRRCLSMVVRATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	103
51 (cont.d)	6318538	49	-AAPKQQSRKAHRGTR--RCLSVVVRATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	104
52 (cont.d)	6318540	49	-AAPKQQSRKAHRGTR--RCLSMVVRATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	104
53 (cont.d)	17736918	46	--S-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	33
54 (cont.d)	6624285	46	-GASAAPKQSRKPHRGNRRCLSMVVRATGS-GGMNLVFVCAEMAPW5KTGGLGDVLGGLP	103
55 (cont.d)	6624281	48	-SAAPKQSRKPHRFDR--RCLSMVVRATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	103
56 (cont.d)	4760580	48	-SAAPKQSRKPHRFDR-RCLSMVVR ATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	103
59 (cont.d)	136765	48	-SAAPKQSRKPHRFDR--RCLSMVVRATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	103
57 (cont.d)	4588609	46	-GASAAPTQSRKAHRGTRRCLSMVVRATGS-GGMNLVFVGAEMAPWSKTGGLGDVLGGLP	103
62 (cont.d)	4588607	36	A--D-AALGIRTVGASAAP--KQSRKPHRGNRRC	64
58 (cont.d)	6136121	57	-AKQSRSLVKKTDNGS--PLGK-IICG--T--GMNLVFVLAEVGPWSKTGGLGDVVGGLP	108
59 (cont.d)	15637079	56	--AKKPSKNGRENEGG--MAAGTIVCK--Q-QGMNLVFVGCEVGPWCKTGGLGDVLGGLP	108
63 (cont.d)	3832512	75	IVCG--N--GMNLVFVGAEVGPWSKTGGLGDVLGGLP	107
64 (cont.d)	2833381	75	IVCK--Q-QGMNLVFVGCEEGPWCKTGGLGDVLGGLP	108
65 (cont.d)	12003285	73	CE--T-SGMTLIFVSAECGPWSKTGGLGDVVGGLP	104
66 (cont.d)	228210	75	IVCG--K--GMNLIFVGTEVGPWSKTGGLGDVLGGLP	107
67 (cont.d)	15626365	82	IIVC-----GMNLIFVGTEVAPWSKTGGLGDVLGGLP	113
68 (cont.d)	267196	75	IVCG--K--GMNLIFVGTEVGPWSKTGGLGDVLGGLP	107
60 (cont.d)	2833388	58	---SKAVKKVSATGNG--RPAAKIICG--H--GMNLIFVGAEVGPWSKTGGLGDVLGGLP	108
69 (cont.d)	602594	75	IVCG--K--GMNLIFVGTEVGPWSKTGGLGDVLGGLP	107
70 (cont.d)	15223331	78	IVC---E-KGMSVIFIGAEVGPWSKTGGLGDVLGGLP	110
71 (cont.d)	5441242	79	GMNLIFVGAEVAPWSKTGGLGDVLGGLP	106
72 (cont.d)	18139611	75	IVC---S-AGMTIIFIATECHPWCKTGGLGDVLGGLP	107
73 (cont.d)	2833383	73	IVC------GMSLVFVGAEVGPWSKTGGLGDVLGGLP	103
74 (cont.d)	6492245	61	--RTPAPIVC---S-TGMPIIFVATEVHPWCKTGGLGDVVGGLP	98

[0379]

TABLE VIII
Maize (GBSS) “GLASS” Domain and it's Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)            “GLASS” Sequence            (ending)	#
75	MAIZE-GESS	104	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	163
76	136757	104	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	163
77	2833385	109	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	168
78	136758	110	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	169
79	2833382	110	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	169
80	297424	110	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	169
81	7798551	110	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	169
82	82478	110	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	169
83	297422	110	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	169
84	136755	103	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	162
85	18652407	103	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	162
86	4760582	105	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	164
87	11037536	105	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	164
88	6624287	104	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	163
89	6624283	105	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	164
90	4760584	104	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	163
91	6318538	105	PAMAANGRRVMVISPRYDQYKDAWDTSVVSEIKVADEYERVRYFHCFKRGVDRVFVDHPC	164
92	6318540	105	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	164
93	17736918	34	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	93
94	6624285	104	AAMAANGHRVMVISPRYDQYKDAWDTSVISEIKVVDRYERVRYFHCYKRGVDRVFVDHPC	163
95	6624281	104	AAMAANGHRVMVISPRYDQYKDAWDTSVISEIKVVDRYERVRYFHCYKRGVDRVFVDHPC	163
96	4760580	104	AAMAANGHRVMVISPRYDQYKDAWDTSVISEIKVVDRYERVRYFHCYKRGVDRVFVDHPC	163
97	136765	104	AAMAANGHRVMVISPRYDQYKDAWDTSVISEIKVVDRYERVRYFHCYKRGVDRVFVDHPC	163
98	4588609	104	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	163
99	4588607	65	LSMVANGHRVMVISPRYDQYKDAWDTSVISEIKVVDRYERVRYFHCYKRGVDRVFVDHPC	124
100	6136121	109	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	168
101	15637079	109	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	168
102	3832512	108	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	167
103	2833381	109	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	168
104	12003285	105	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	164
105	228210	108	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	167
106	15626365	114	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	173
107	267196	108	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	167
108	2833388	109	PAMAANGHRVMVVSPRYDQYKDAWDTSVVSEIKMGDGYETVRFFHCYKRGVDRVFVDHPL	168
109	602594	108	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	167
110	15223331	111	PALAARGHRVMTVCPRYDQYKDAWDTCVWELQVGDRIEPVRFFHSYKRGVDRVFVDHPM	170
111	5441242	107	SALAEHGHRVMTVSPRYDQYKDAWDTNVTVEVKVADRIETVRFFHCYKQGVDRVFVDHPC	166
112	18139611	108	PALAAMGHRVMTIVPRYDQYKDAWDTNVLVEVNIGDRTETVRFFHCYKRGVDRVFVDHPM	167
113	2833383	104	PVLAGNGHRVMTVSPRYDQYKDAWDTNVLVEVKVGDKIETVRFFHCYKRGVDRVFVDHPL	163
114	6492245	99	PALAAMGHRVMTIAPRYDQYKDTWDTNVLVEVIVGDRTETVRFFHCYKRGVDRVFVDHPM	158
115	9587307	12	PAMAANGHRVMTISPRYDQYKDAWDTEVTVELKVGDKTETVRFFHCYKRGVDRVFVDHPM	71
116	9587337	12	PANAANGHRVMTISPRYDQYKDAWDTEVTVELKVGDKIETVRFFHCYKRGVDRVFVDHPL	71
117	9587327	12	PAMAANGHRVMTISPRYDQYKDAWDTEVTVELKVGEKIEFVRFFHCYKRGVDRVFVDHPL	71
118	9587333	12	PANAANGHRVMTVSPRYDQYKDAWDTEVTVELKVGQKIETVRFFHCHKRGVDRVFVDHPL	71
119	9587329	12	PALAANGHRVMTVSPRYDQYKDAWDTNVLVEIEVGGKIETVRFFHCYKRGVDRVFVDHPL	71
120	9587325	12	PAMAANGHRVMTISPRYDQYKDAWDTEVTVELNVGEKTETVRFFECYKRGVDRVFVDHPL	71
75	MAIZE-GESS	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
76	136757	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
77	2833385	169	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	223
78	136758	170	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	224
79	2833382	170	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	224
80	297424	170	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	224
81	7798551	170	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	224
82	82478	170	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	224
83	297422	170	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQ---EAPRILSLNNNPYFSGPY-----	221
84	136755	163	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	217
85	18652407	163	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	217
86	4760582	165	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	219
87	11037536	165	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	219
88	6624287	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
89	6624283	165	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	219
90	4760584	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
91	6318538	165	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	219
92	6318540	165	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	219
93	17736918	94	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	148
94	6624285	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
95	6624281	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
96	4760580	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
97	136765	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPYAMLCR	223
98	4588609	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
99	4588607	125	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	179
100	6136121	169	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	223
101	15637079	169	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	223
102	3832512	168	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	222
103	2833381	169	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	223
104	12003285	165	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	219
105	228210	168	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	222
106	15626365	174	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	228
107	267196	168	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	222
108	2833388	169	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	223
109	602594	168	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	222
110	15223331	171	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	225
111	5441242	167	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	221
112	18139611	168	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	222
113	2833383	164	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	218
114	6492245	159	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	213
115	9587307	72	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	126
116	9587337	72	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	126
117	9587327	72	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	126
118	9587333	72	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	126
119	9587329	72	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	126
120	9587325	72	FLERVWGKTEEKIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPY-----	126
75	MAIZE-GESS	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
76	136757	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
77	2833385	224	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	277
78	136758	225	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	278
79	2833382	225	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	278
80	297424	225	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	278
81	7798551	225	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	278
82	82478	225	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	278
83	297422	222	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	275
84	136755	218	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	271
85	18652407	218	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	271
86	4760582	220	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	273
87	11037536	220	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	273
88	6624287	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
89	6624283	220	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	273
90	4760584	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
91	6318538	220	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	273
92	6318540	220	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	273
93	17736918	149	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	202
94	6624285	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
95	6624281	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
96	4760580	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
97	136765	224	AVPRRAGEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	283
98	4588609	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
99	4588607	180	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	233
100	6136121	224	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	277
101	15637079	224	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	277
102	3832512	223	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	276
103	2833381	224	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	277
104	12003285	220	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	273
105	228210	223	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	276
106	15626365	229	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	282
107	267196	223	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	276
108	2833388	224	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	277
109	602594	223	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	276
110	15223331	226	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	279
111	5441242	222	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	275
112	18139611	223	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	276
113	2833383	219	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	272
114	6492245	214	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	267
115	9587307	127	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	180
116	9587337	127	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	180
117	9587327	127	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	180
118	9587333	127	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	180
119	9587329	127	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	180
120	9587325	127	------GEDVVFVCNDWHTGPLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYP	180

[0380]

TABLE IX
Maize (GBSS) “LINKR” Domain and it's Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)             “LINKR” Sequence             (ending)	#
121	MAIZE GESS	273	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	332
122	136757	273	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	332
123	2833385	278	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	337
124	136758	279	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	338
125	2833382	279	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	338
126	297424	279	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	338
127	7798551	279	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	338
128	82478	279	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	338
129	297422	276	ELNLPERFKSSFDFIDGYEKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCE	335
130	136755	272	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	331
131	118652407	272	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	331
132	4760582	274	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	333
133	11037536	274	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	333
134	6624287	273	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	332
135	6624283	274	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	333
136	4760584	273	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	332
137	6318538	274	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	333
138	6318540	274	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	333
139	17736918	203	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	262
140	6624285	273	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	332
141	6624281	273	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	332
142	4760580	273	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	332
143	136765	284	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	343
144	4588609	273	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	332
145	4588607	234	QLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCE	293
146	6136121	278	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	337
147	15637079	278	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	337
148	3832512	277	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	336
149	2833381	278	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	337
150	12003285	274	KLNLPDQLKSSFDFMDGYEKPVKGRKINWMKAGIIESDRVLTVSPYYANELVSGPDKGVE	333
151	228210	277	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	336
152	15626365	283	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	342
153	267196	277	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	336
154	2833388	278	RLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	337
155	602594	277	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	336
156	15223331	280	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	339
157	5441242	276	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	335
158	18139611	277	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	336
159	2833383	273	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	332
160	6492245	268	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	327
161	9587307	181	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	240
162	9587337	181	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	240
163	9587327	181	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	240
164	9587333	181	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	240
165	9587329	181	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	240
166	9587325	181	LLNLPDQFKSSFDFFDGYEKPVKGRKINWMKAGILESDRVVTVSPYYAKELVSGPDKGVE	240
121	MAIZE GESS	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
122	136757	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
123	2833385	338	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	396
124	136758	339	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	397
125	2833382	339	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	397
126	297424	339	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	397
127	7798551	339	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	397
128	82478	339	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	397
129	297422	336	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	394
130	136755	332	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	390
131	18652407	332	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	390
132	4760582	334	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	392
133	11037536	334	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	392
134	6624287	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
135	662428	334	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	392
136	4760584	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
137	6318538	334	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	392
138	6318540	334	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	392
139	17736918	263	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	321
140	6624285	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
141	6624281	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
142	4760580	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
143	136765	344	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	402
144	4588609	333	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	391
145	4588607	294	LDNIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	352
146	6136121	338	LDNVIAKTSI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	396
147	15637079	338	LDNHIRDCGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	396
148	3832512	337	LDNIIRSIGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	395
149	2833381	338	LDNHIRDCGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	396
150	12003285	334	LDNILRKCTV-TGIVNGMDTQEWNPATDKYIDNHYDITTDGKPLLKEALQAEVGLPVD	392
151	228210	337	LDSVLRKTCI-TGIVNGMDTQEWNPATDKYIDNHYDITTDGKPLLKEALQAEVGLPVD	395
152	15626365	343	LDNILRRVGV-TGIVNGMDTQEWNPATDKYIDNHYDITTDGKPLLKEALQAEVGLPVD	401
153	267196	337	LDSVLRKTCI-TGIVNGMDTQEWNPATDKYIDNHYDITTDGKPLLKEALQAEVGLPVD	395
154	2833388	338	LDNFIRKTGI-AGIINGMDVQEWNPVTDKYIDIHYDATTVMDAKPLLQAEVGLPVD	396
155	602594	337	LDSVLRKTCI-TGIVNGMDTQEWNPATDKYIDNHYDITTDGKPLLKEALQAEVGLPVD	395
156	15223331	340	LHKYLRMKTV-SGIINGMDVQEWNPSTDKYIDIKYDITTVTDAKPLIKEALQAAVGLPVD	398
157	5441242	336	LDNIIRKTGV-AGIVNGMDIREWSPKTDKFIDIHFDTTSVKEAKFLLQAEVGLPVN	394
158	18139611	337	LDGILRTKPLETGIVNGMDVYEWNPATDQYISVKYDATTVTEARALNKEMLQAEVGLPVD	396
159	2833383	333	LDNIIRSTGI-IGIVNGMDNREWSPQTDRYIDETTVTEAKPLLKGTLQAEIGLPVD	391
160	6492245	328	LDGVLRAKPLETGIVNGMDVVDWNPATDKYISVKYNATAKEILQAEVGLPVD	387
161	9587307	241	LDNIIRKTGI-QFGIVNGMVQEWNPLTDKYTTVKYDASTVADAKPLLKEALQAEVGLPVD	299
162	9587337	241	LDNIIRKTGI-QFGIVNGMVQEWNPLTDKYTTVKYDASTVADAKPLLKEALQAEVGLPVD	299
163	9587327	241	LDNIIRKTGI-QFGIVNGMVQEWNPLTDKYTTVKYDASTVADAKPLLKEALQAEVGLPVD	299
164	9587333	241	LDNIIRKTGI-QFGIVNGMVQEWNPLTDKYTTVKYDASTVADAKPLLKEALQAEVGLPVD	299
167	150549	221	NIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	57
165	9587329	241	NIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	299
168	15054954	1	NIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	57
169	15054970	1	NIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	57
170	15054956	1	NIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	57
171	15054960	1	NIMRLTGI-TGIVNGMDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVD	57
166	958732524	1	LDNIIRKTGI-RGIVNGMDVQEWNPLTDKYTIVKYDASTVTDAKPLLKEASQAEVGLFVD	299

[0381]

TABLE X
Maize (GBSS) “GLYTR” Domain and it's Alignments with other proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)             “GLYTR” Sequence             (ending)	#
172	MAIZE GBSS	392	RNIPLVAFIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFERMLMSAEEKFPG	451
173	136757	392	RNIPLVAFIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFERMLMSAEEKFPG	451
174	2833385	397	RKIPLVAFIGRLEEQKGPDVMAAAIPQLME--EDVQIVLLGTGKKKFERMLMSAEEKFPG	454
175	136758	398	RKIPLVAFIGRLEEQKGPDVMAAAIPQLMQ--EDVQIVLLGTGKKKFERMLMSAEEKFPG	455
176	2833382	398	RKIPLVAFIGRLEEQKGPDVMAAAIPQLMQ--EDVQIVLLGTGKKKFERMLMSAEEKFPG	455
177	297424	398	RKIPLVAFIGRLEEQKGPDVMAAAIPQLMQ--EDVQIVLLGTGKKKFERMLMSAEEKFPG	455
178	7798551	398	RKIPLVAFIGRLEEQKGPDVMAAAIPQLMQ--EDVQIVLLGTGKKKFERMLMSAEEKFPG	455
179	82478	398	RKIPLIAFIGRLEEQKGPDVMAAAIPELMQ--EDVQIVLLGTGKKKFEKLLKSMEEKYPG	455
180	297422	395	RKVPLIAFIGRLEEQKGPDVMAAAIPELMQ--ENVQIVLLGTGKKKFEKLLKSMEEKYPG	452
181	136755	391	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPG	449
182	18652407	391	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPG	449
183	4760582	393	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPD	451
184	11037536	393	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPS	451
185	6624287	392	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPN	450
186	6624283	393	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPS	451
187	4760584	392	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPS	450
188	6318538	393	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPS	451
189	6318540	393	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPS	451
190	17736918	322	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPS	380
191	6624285	392	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPT	450
192	6624281	392	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPT	450
193	4760580	392	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPT	450
194	136765	403	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPT	461
195	4588609	392	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPS	450
196	4588607	353	RKVFLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIILLGTGKKKFEKLLKSMEEKFPT	411
197	6136121	397	KNIPVIGFIGRLEEQKGSDILVAAISKFVGL--DVQIIILGTGKKKFEQQIQELEVLYPD	454
198	15637079	397	RNIPLIGFIGRLEEQKGSDILYAAISKFISM--DVQILILGTGKKKFEQQIEQLEVMYPD	454
199	3832512	396	RNIPVIGFIGRLEEQKGSDILVESIPKFID--QNVQIIVLGTGKKIMEKQIEQLEVTYPG	453
200	2833381	397	RNIPLIGFIGRLEEQKGSDILYAAISKFISM--DVQILILGTGKKKFEQQIEQLEVMYPD	454
201	12003285	393	PNVPLVGFIGRLEEQKGSDILVAALHKFIEM--DVQVVILGTGKKEFEKQIEQLEELYPG	450
202	228210	396	KKIPLIGFIGRLEEQKGSDILVAAIHKFIGL--DVQIVVLGTGKKEFEQEIEQLEVLYPG	453
203	15626365	402	KNVPLIAFIGRLEEQKGSDILVEAIPQFIK--ENVQIVALGTGKKEMEKQLQQLEISYPD	459
204	267196	396	KKIPLIGFIGRLEEQKGSDILVAAIHKFIGL--DVQIVVLGTGKKEFEQEIEQLEVLYPN	453
205	2833388	397	PNVPLIGFIGRLEEQKGSDIFVAAISQLVE--HNVQIVILGTGKKKFEKQIEHLEVLYPD	454
206	602594	396	KKVPLIGFIGRLEEQKGSDILVAAIHKFIGL--DVQIVVLGTGKKEFEQEIEQLEVLYPN	453
207	15223331	399	RDVPVIGFIGRLEEQKGSDILVEAISKFMGL--NVQMVILGTGKKKMEAQILELEEKFFG	456
208	5441242	395	RDIPLIGFIGRLEEQKGSDILVEAIPKFID--QNVQIIILGTGKKSMEKQIEQLEEIYPE	452
209	18139611	397	SSIPLIVFVGRLEEQKGSDILIAAIPEFVE--GNVQIIVLGTGKKKMEEELILLEVKYPN	454
210	2833383	392	SSIPLIGFIGRLEEQKGSDILVEAIAKFAD--ENVQIVVLGTGKKIMEKQIEVLEEKYPG	449
211	6492245	388	SSIFVIVFIGRLEEQKGSDILIAAIPEFLE--ENVQIIVLGTGKKKMEEELMLLEAKYPQ	445
212	9587307	300	RDIPVIGFIGRLEE	313
213	9587337	300	RDIFVIGFIGRLEE	313
214	9587327	300	KDIPVIGFIGRLEE	313
215	9587333	300	KMIPVIGFIGRLEE	313
216	15054922	58	RNIPLVAFIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFEPMLMSAEEKFFG	117
217	9587329	300	RDIPVIGFIGRLEE	313
218	15054954	58	RNIPLVAFIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFERMLMSAEEKFPG	117
219	15054970	58	RNIPLVAFIGRLEEQKGPDVMAAAIPQLMDMVEDVQIVLLGTGKKKFERMLMSAEEKFPG	117
220	15054956	58	PNIPLVAEIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFERMLMSAEEKFPG	117
221	15054960	58	RNIPLVAFIGRLEEQKGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFERMLMSAEEKFPG	117
222	9587325	300	RDIPVIGFIGRLEE	313
172	MAIZE GBSS	452	KVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIE	511
173	136757	452	KVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIE	511
174	2833385	455	KVRAVVKFNAPLAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIE	514
175	136758	456	KVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGGLVDTVIE	515
176	2833382	456	KVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGGLVDTVIE	515
177	297424	456	KVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGGLVDTVIE	515
178	7798551	456	KVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGGLVDTVIE	515
179	82478	456	KVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGGLVDTVIE	515
180	297422	453	KVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGGLVDTVIE	512
181	136755	450	KVRAVVRFNAPLAHQMMAGADLLAVTSRFEPCGLIQLQGMRYGTPCVCASTGGLVDTIVE	509
182	18652407	450	KVRAVVRFNAPLAHQMMAGADLLAVTSRFEPCGLIQLQGMRYGTPCVCASTGGLVDTIVE	509
183	4760582	452	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIME	511
184	11037536	452	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIME	511
185	6624287	451	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	510
186	6624283	452	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	511
187	4760584	451	KVPAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	510
188	6318538	452	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	511
189	6318540	452	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	511
190	17736918	381	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	440
191	6624285	451	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	510
192	6624281	451	KVRAVVRFNAPLAHQNMAGADVIAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	510
193	4760580	451	KVWAVVRFNAPLAHQNMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	510
194	136765	462	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	521
195	4588609	451	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	510
196	4588607	412	KVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIVE	471
197	6136121	455	KARGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHANRYGTIPICASTGGLVDTVTE	514
198	15637079	455	KARGVAKFNVPLAHMITAGADFMLIPSRFEPCGLIQLHANRYGTPCICASTGGLVDTVKE	514
199	3832512	454	KAIGVAKFNSPLAHKIIAGADFIVIPSRFEPCGLVQLHAMPYGTVPIVSSTGGLVDTVKE	513
200	2833381	455	KARGVAKFNVPLAHMITAGADFMLIPSRFEPCGLIQLHAMPYGTPCICASTGGLVDTVKE	514
201	12003285	451	KAVGVAKFNVPLAHKITAGADFMLVPSRFEPCGLIQLHAMPYGTIPICASTGGLVDTVKE	510
202	228210	454	KVKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTGGLVDTVKE	513
203	15626365	460	KARGVAKFNVPLAHMMIAGADFILIPSRFEPCGLIQLQAMRYGTVPIVASTGGLVDTVKE	519
204	267196	454	KAKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTGGLVDTVKE	513
205	2833388	455	KARGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVKE	514
206	602594	454	KAKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTGGLVDTVKE	513
207	15223331	457	KAVGVAKFNVPLAHMITAGADFIIVPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVKD	516
208	5441242	453	KARGIAKFDGPLAHKIIAGSDFIMIPSRFEPCGLVQLHSMPYGTVPIVSSTGGLVDTVQE	512
209	18139611	455	TARGLAKFNVPLAHMMFAGADFIIVPSRFEPCGLIQLQGMRYGVVPICSSTGGLVDTVKE	514
210	2833383	450	KAIGITKFNSPLAHKIIAGADFIVIPSRFEPCGLVQLHAMPYGTVPIVSSTGGLVDTVKE	509
211	6492245	446	NARGIAKFNVPLAHMMFAGANFIIVPSRFEPCGLIQLQGMRYGVIPICSSTGGLVDTVSE	505
216	15054922	118	KVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIE	177
218	15054954	118	KVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACSSTGGLVDTIIE	177
219	15054970	118	KVRAVVKFNAALAHHINAGADVIAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIE	177
220	15054956	118	KVRAVVKFNAALAHNIMAGADVIAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIE	177
221	15054960	118	KVRAVVKFNAALAHHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIE	177
172	MAIZE GBSS	512	GKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIKVVGTPAYEEMVRNCMIQDLSWKGPAK	571
173	136757	512	GKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIKVVGTPAYEEMVRNCMIQDLSWKGPAK	571
174	2833385	515	GKTGFHMGRLSVDCNVVEPADVKKVATTLKRAIKVVGTPAYEEMVKNCMIQDLSWKGPAK	574
175	136758	516	GKTGFHMGRLSVDCKVVEPSDVKKVAATLKRAIKVGTPAYEEMVVRNCMIQDLSWKGPAK	575
176	2833382	516	GKTGFHMGRLSVNCKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQDLSWKGPAK	575
177	297424	516	GKTGFHMGRLSVDCKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQDLSWKGPAK	575
178	7798551	516	GKTGFHMGRLSVDCKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQDLSWKGPAK	575
179	82478	516	GKTGFHMGRLSVDGKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQDLSWKGPAK	575
180	297422	513	GKTGFHMGRLSVDGKVVEPSDVQKVATTLKRAIKIVGTPAYNEMVRNCNNQDLSWKGPAK	572
181	136755	510	GKTGFHMGRLSVDCNVVEPADVKKVATTLKRAVKVVGTPAYQEMVKNCMIQDLSWKGPAK	569
182	18652407	510	GKTGFHMGRLSVDCNVVEPADVKKVATTLKRAVKVVGTPAYQEMVKNCMIQDLSWKGPAK	569
183	4760582	512	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	571
184	11037536	512	GKTGFHMGHLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	571
185	6624287	511	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHGMVKNCMIQDLSWKGPAK	570
186	6624283	512	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	571
187	4760584	511	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	570
188	6318538	512	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	571
189	6318540	512	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	571
190	17736918	441	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	500
191	6624285	511	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	570
192	6624281	511	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	570
193	4760580	511	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	570
194	136765	522	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	581
195	4588609	511	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKFAVKVVGTPAYHEMVKNCMIQDLSWKGPA	569
196	4588607	472	GKTGFHMGRLSVDCNVVEPADVKKVVTTLKRAVKVVGTPAYHEMVKNCMIQDLSWKGPAK	531
197	6136121	515	GFTGFHMGAFNVECATVDPADVQKIATTVERALAAYGSVAYKEMIQNCMAQDLSWKGPAK	574
198	15637079	515	GYTGFHMGAFNVDCETVDPEDVLKVITTVGRALAMYGTLAFTEMIKNCMSQELSWKGPAK	574
199	3832512	514	GYTGFHVGAFSVECEAVDPADVEKLATTVNRALKTYGTQALKEMILNCMAQDFSWKGPAK	573
200	2833381	515	GYTGFHMGAFNVDCETVDPEDVLKVITTVGRALAIYGTLAFTEMIKNCMSQELSWKGPAK	574
201	12003285	511	GFTGFHMGAFNVECDAVDPADVLKIVKTVGRALEVYGTPAFREMINNCMSLDLSWKGPAK	570
202	228210	514	GYTGFHMGAFNVECDVVDPADVLKIVTTVARALAVYGTLAFAEMIKNCMSEELSWKEPAK	573
203	15626365	520	GFTGFHMGSFNVKCDAVDPVDVDAIPKTVTKALGVYGTSAFAEMIKNCMAQELSWKGPAK	579
204	267196	514	GYTGFHMGAFNVECDVVDPADVLKIVTTVARALAVYGTLAFAEMIKNCMSEELSWKEPAK	573
205	2833388	515	GYTGFQMGALVECDKIDSAflVAAIVKTVARALGTYATAALREMILNCMAQDLSWKGPAR	574
206	602594	514	GYTGFHNGAFNVECDVVDPADVLKIVTTVARALAVYGTLAFAEMIKNCMSEELSWKEPAK	573
207	15223331	517	GYTGFHIGRFNVKCEVVDPDDVIATAKAVTRAVAVYGTSAMQEMVKNCMDQDFSWKGPAR	576
208	5441242	513	GFTGFHMGAFNVDCEAIDPADVEKIATTVRRALGTYGTVAMEKIIQNCMAQDFSWKGPAK	572
209	18139611	515	GVTGFHMGLFNVECETVDPVDVTAVASTVKRALKQYNTPAFQEMVQNCMAQDLSWKGPAK	574
210	2833383	510	GYTGFHAGPFDVECEDVDPDDVDKLAATVKRALKTYGTQAMKQIILNCMAQNFSWKKPAK	569
211	6492245	506	GVTGFHMGSFNVEFETVDPADVAAVASNVTRALKQYKTPSFHANVQNCMAQDLSWKGPAK	565
212	15054922	178	GKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIKVVGTPAYEEMVFNCMIQDLSWKGPAK	237
218	15054954	178	GKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIKVVGTPAYEEMVPNCMIQDLSWKGPAK	237
219	15054970	178	GKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIKVVGTPAYEEMVRNCMIQDLSWKGPAK	237
220	15054956	178	GKTGFHMGRFSVDCNVVEPADVKKVATTLQRAIKVVGTPAYEEMVRNCMIQDLSWKGPAK	237
221	15054960	178	GKTGFHMGRLSVDCNVVEPADVKKVATTLQRAIKVVGTPVYEEMVRNCMIQDLSWKGPAK	237

[0382]

TABLE XI
Maize (GBSS) “CTEND” Domain and it's Alignments with other proteins
SEQ Id.No.	Accession Number	a.a #	(start) “CTEND” Sequence (ending)	a.a. #
223	Maize GESS	572	NWENVLLSLXXXXXXXXXXXXXXXXLAKENVAAP	605
224	136757	572	NWENVLLSLGVAGGEPGVEGEEIAPLAKENVAAP	605
225	2833385	575	NWENVLLSLGVAGGEPGIEGEEIAPLAKENVAAP	608
226	136758	576	NWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP	609
227	2833382	576	NWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP	609
228	297424	576	NWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP	609
229	7798551	576	NWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP	609
230	82478	576	NWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP	609
231	297422	573	NWENVLLGLGVAGSEPGVEGEEIAPLAKENVAAP	606
232	136755	570	NWEDVLLELGVEGSEPGIVGEEIAPLAKENVAAP	603
233	18652407	570	NWEDVLLELGVEGSEPGIVGEEIAPLAMENVAAP	603
234	4760582	572	NWEDVLLELGVEGSEPGVIGEEIAPLAMENVAAP	605
235	11037536	572	NWEDVLLELGVEGSEPGVIGEEIAPLAMENVAAP	605
236	6624287	571	NWEDVLLELGVEGSEPGVIGEEIAPLAMENVAAP	604
237	6624283	572	NWEDVLLELGVEGSEPGVIGEEIAPLAMENVAAP	605
238	4760584	571	NWEDVLLELGVEGSEPGVIGEEIAPLAMENVAAP	604
239	6318538	572	NWEHThLELGVEGSEPGIVGEEIAPLAMENVAAP	605
240	6318540	572	NWEDVLLELGVEGSEPGVIGEEIAPLAMENVAAP	605
241	17736918	501	NWEDVLLELGVEGSEPGVIGEEIAPLANENVAAP	534
242	6624285	571	NWEDVLLELGVEGSEPGIVGEEIAPLALENVAAP	604
243	6624281	571	NWEDVLLELGVEGSEPGIVGEEIAPLALENVAAP	604
244	4760580	571	NWEDVLLELGVEGSEPGIVGEEIAPLALENVAAP	604
245	136765	582	NWEDVLLELGVEGSEPGIVGEEIAPLALENVAAP	615
246	4588607	532	NWEDVLLELGVEGSEPGIVGEEIAPLALENVAAP	565
247	6136121	575	NWEKMLLSLGVSGSEPGVDGEEIAPLAKENVATP	608
248	15637079	575	NWETVLLSLGVAGSEPGVEGDEIAPLAKENVATP	608
249	3832512	574	QWEQALLSLEVAGSEPGIDGEEVAPLAKENVATP	607
250	2833381	575	NWETVLLSLGVAGSEPGVEGEEIAPLAKENVATP	608
251	12003285	571	NWETVLLSLGVAGSEPGVEGDEIAPLAKENVATP	604
252	228210	574	KWETLLLGLGASGSEPGVEGEEIAPLAKENVATP	607
253	15626365	580	KWEEVLLNLGVPDSEPGIDGQEIAPQAKENVATP	613
254	267196	574	KWETLLLGLGASGSEPGVEGEEIAPLAKENVATP	607
255	2833388	575	MWEKMLLDLEVTGSEPGTEGEEIAPLAKENVPTP	608
256	602594	574	KWETLLLGLGASGSEPGVEGEEIAPLAKENVATP	607
257	1522333	577	LWEKVLLSLNVAGSEAGTEGEEIAPLAKENVATP	610
258	5441242	573	QWEKVLFSLDVGRSEAGIEGDEIAPLAKENVATP	606
259	18139611	575	KWEEVLLGLGVEGSQPGIEGEEVAPLAKENVATP	608
260	2833383	570	LWEKALLNLEVTGNVAGIDGDEIAPLAKENVATP	603
261	6492245	566	KWEEALLGLGVEGSQPGIEGEEIAPLAKQNVATP	599
262	15054922	238	NWENVLLSLGVAGGEPGVEGEEIAPLAKENVAAP	271
263	15054954	238	NWENVLLSLGVAGGEPGVEGEEIAPLAKENVAAP	271
264	15054970	238	NWENVLLSLGVAGGEPGVEGEEIAPLAKENVAAP	271
265	15054956	238	NWENVLLSLGVAGGEPGVEGEEIAPLAKENVAAP	271
266	15054960	238	NWENVLLSLGVAGGEPGVEGEEIAPLAKENVAAP	271

[0383]

TABLE XII
Identities of the Accession Numbers used in Table Numbers VIII-XI.
Accession	Brief Description of sequences
Id.	producing significant alignments	score	E-value
gi|136757|sp|P04713|UGST MAIZE	Granule-bound glycogen [star . . .	1125	0.0
gi|2833385|sp|Q43134|UGST SORBI	Granule-bound glycogen [sta . . .	1062	0.0
gi|136758|sp|P19395|UGST ORYSA	Granule-bound glycogen [star . . .	959	0.0
gi|2833382|sp|Q42968|UGST ORYGL	Granule-bound glycogen [sta . . .	957	0.0
gi|297424|emb|CAA46294.1|	(X65183) glycogen (starch) syntha . . .	956	0.0
gi|7798551|gb|AAC61675.2|	(AF031162) granule-bound starch s . . .	956	0.0
gi|82478|pir|JQ0703	UDPglucose--starch glucosyltransferase . . .	951	0.0
gi|297422|emb|CAA45472.1|	(X64108) starch granule-bound sta . . .	945	0.0
gi|136755|sp|P09842|UGST HORVU	Granule-bound glycogen [star . . .	944	0.0
gi|18652407|gb|AAL77109.1|AF474373_6	(AF474373) granule-bou . . .	943	0.0
gi|4760582|dbj|BAA77351.1|	(AB019623) starch synthase (GBSS . . .	939	0.0
gi|11037536|gb|AAG27624.1|AF286320_1	(AF286320) granule bou . . .	937	0.0
gi|6624287|dbj|BAA88512.1|	(AB029064) starch synthase (GBSS . . .	936	0.0
gi|6624283|dbj|BAA88510.1|	(AB029062) starch synthase (GBSS . . .	935	0.0
gi|4760584|dbj|BAA77352.1|	(AB019624) starch synthase (GBSS . . .	935	0.0
gi|6318538|gb|AAF06936.1|AF110373_1	(AF110373) granule-boun . . .	932	0.0
gi|6318540|gb|AAF06937.1|AF110374_1	(AF110374) granule-boun . . .	930	0.0
gi|17736918|gb|AAL41028.1|	(AF250137) mutant granule bound . . .	929	0.0
gi|6624285|dbj|BAA88511.1|	(AB029063) starch synthase (GBSS . . .	926	0.0
gi|6624281|dbj|BAA88509.1|	(AB029061) starch synthase (GBSS . . .	922	0.0
gi|4760580|dbj|BAA77350.1|	(AB019622) starch synthase (GBSS . . .	921	0.0
gi|136765|sp|P27736|UGST WHEAT	Granule-bound glycogen [star . . .	915	0.0
gi|4588609|gb|AAD26156.1|AF113844_1	(AF113844) granule-boun . . .	910	0.0
gi|4588607|gb|AAD26155.1|AF113843_1	(AF113843) granule-boun . . .	889	0.0
gi|6136121|sp|O82627|UGST ANTMA	Granule-bound glycogen [sta . . .	773	0.0
gi|15637079|dbj|BAB68126.1|	(AB071604) granule-bound starch . . .	771	0.0
gi|3832512|gb|AAC70779.1|	(AF097922) granule-bound glycogen . . .	765	0.0
gi|2833381|sp|Q42857|UGST IPOBA	Granule-bound glycogen [sta . . .	764	0.0
gi|12003285|gb|AAG43519.1|AF210699_1	(AF210699) granule-bou . . .	762	0.0
gi|228210|prf||1718316A	granule-bound starch synthase [Sola . . .	758	0.0
gi|15626365|emb|CAC69955.1|	(AJ345045) granule-bound starch . . .	756	0.0
gi|267196|sp|Q00775|UGST SOLTU	Granule-bound glycogen [star . . .	754	0.0
gi|2833388|sp|Q43784|UGST MANES	Granule-bound glycogen [sta . . .	754	0.0
gi|602594|emb|CAA58220.1|	(X83220) starch (bacterial glycog . . .	752	0.0
gi|15223331|ref|NP 174566.1|	(NM_103023) starch synthase, p . . .	742	0.0
gi|5441242|dbj|BAA82346.1|	(AB029546) granule-bound starch . . .	734	0.0
gi|18139611|gb|AAL58572.1|	(AY069940) granule binding starc . . .	729	0.0
gi|2833383|sp|Q43092|UGST PEA	Granule-bound glycogen [starc . . .	726	0.0
gi|6492245|gb|AAF14233.1|AF109395_1	(AF109395) granule-boun . . .	716	0.0
gi|9587307|gb|AAF89255.1|AF285980_1	(AF285980) granule-boun . . .	518	e−146
gi|9587337|gb|AAF89270.1|AF285995_1	(AF285995) granule-boun . . .	517	e−145
gi|9587327|gb|AAF89265.1|AF285990_1	(AF285990) granule-boun . . .	514	e−145
gi|9587333|gb|AAF89268.1|AF285993_1	(AF285993) granule-boun . . .	514	e−144
gi|15054922|gb|AAK82769.1|AF292500_1	(AF292500) granule-bou . . .	513	e−144
gi|9587329|gb|AAF89266.1|AF285991_1	(AF285991) granule-boun . . .	513	e−144
gi|15054954|gb|AAK82785.1|AF292516_1	(AF292516) granule-bou . . .	513	e−144
gi|15054970|gb|AAK82793.1|AF292524_1	(AF292524) granule-bou . . .	512	e−144
gi|15054956|gb|AAK82786.1|AF292517_1	(AF292517) granule-bou . . .	512	e−144
gi|15054960|gb|AAK82788.1|AF292519_1	(AF292519) granule-bou . . .	512	e−144
gi|9587325|gb|AAF89264.1|AF285989_1	(AF285989) granule-boun . . .	511	e−144
gi|3493047|gb|AAD02981.1|	(AF079261) granule-bound starch s . . .	511	e−144
gi|15054930|gb|AAK82773.1|AF292504_1	(AF292504) granule-bou . . .	511	e−144
gi|9587313|gb|AFF89258.1|AF285983_1	(AF285983) granule-boun . . .	509	e−143
gi|9587352|gb|AAF89276.1|AF286003_1	(AF286003) granule-boun . . .	509	e−143
gi|15054968|gb|AAK82792.1|AF292523_1	(AF292523) granule-bou . . .	509	e−143
gi|9587331|gb|AAF89267.1|AF285992_1	(AF285992) granule-boun . . .	509	e−143
gi|9587323|gb|AAF89263.1|AF285988_1	(AF285988) granule-boun . . .	509	e−143
gi|9587299|gb|AAF89251.1|AF285976_1	(AF285976) granule-boun . . .	507	e−142
gi|15054940|gb|AAK82778.1|AF292509_1	(AF292509) granule-bou . . .	507	e−142
gi|3493043|gb|AAD02979.1|	(AF079259) granule-bound starch s . . .	506	e−142
gi|9587305|gb|AAF89254.1|AF285979_1	(AF285979) granule-boun . . .	506	e−142
gi|3493045|gb|AAD02980.1|	(AF079260) granule-bound starch s . . .	505	e−142
gi|9587319|gb|AAF89261.1|AF285986_1	(AF285986) granule-boun . . .	504	e−142
gi|9587311|gb|AAF89257.1|AF285982_1	(AF285982) granule-boun . . .	504	e−141
gi|9587343|gb|AAF89273.1|AF285998_1	(AF285998) granule-boun . . .	503	e−141
gi|9587297|gb|AAF89250.1|AF285975_1	(AF285975) granule-boun . . .	503	e−141
gi|15054984|gb|AAK82800.1|AF292531_1	(AF292531) granule-bou . . .	503	e−141
gi|9587339|gb|AAF89271.1|AF285996_1	(AF285996) granule-boun . . .	502	e−141
gi|9587303|gb|AAF89253.1|AF285978_1	(AF285978) granule-boun . . .	501	e−141
gi|15054990|gb|AAK82803.1|AF292534_1	(AF292534) granule-bou . . .	501	e−141
gi|9587317|gb|AAF89260.1|AF285985_1	(AF285985) granule-boun . . .	500	e−140
gi|9587341|gb|AAF89272.1|AF285997_1	(AF285997) granule-boun . . .	500	e−140
gi|9587348|gb|AAF89274.1|	(AF286001) granule-bound starch s . . .	499	e−140
gi|9587293|gb|AAF89248.1|AF285973_1	(AF285973) granule-boun . . .	499	e−140
gi|9587335|gb|AAF89269.1|AF285994_1	(AF285994) granule-boun . . .	497	e−139
gi|9587321|gb|AAF89262.1|AF285987_1	(AF285987) granule-boun . . .	497	e−139
gi|9587309|gb|AAF89256.1|AF285981_1	(AF285981) granule-boun . . .	493	e−138
gi|9587301|gb|AAF89252.1|AF285977_1	(AF285977) granule-boun . . .	493	e−138
gi|9587295|gb|AAF89249.1|AF285974_1	(AF285974) granule-boun . . .	492	e−138
gi|3493007|gb|AAD02961.1|	(AF079241) granule-bound starch s . . .	489	e−137
gi|3493041|gb|AAD02978.1|	(AF079258) granule-bound starch s . . .	489	e−137
gi|3493019|gb|AAD02967.1|	(AF079247) granule-bound starch s . . .	488	e−137
gi|3493021|gb|AAD02968.1|	(AF079248) granule-bound starch s . . .	488	e−137
gi|3493005|gb|AAD02960.1|	(AF079240) granule-bound starch s . . .	488	e−136
gi|3493035|gb|AAD02975.1|	(AF079255) granule-bound starch s . . .	487	e−136
gi|3492995|gb|AAD02955.1|	(AF079235) granule-bound starch s . . .	487	e−136
gi|3493037|gb|AAD02976.1|	(AF079256) granule-bound starch s . . .	487	e−136
gi|3493025|gb|AAD02970.1|	(AF079250) granule-bound starch s . . .	486	e−136
gi|3493023|gb|AAD02969.1|	(AF079249) granule-bound starch s . . .	486	e−136
gi|16716335|gb|AAC17969.3|	(AF026420) granule-bound starch . . .	485	e−136
gi|3493011|gb|AAD02963.1|	(AF079243) granule-bound starch s . . .	485	e−136
gi|13377473|gb|AAK20725.1|	(AF318769) granule-bound starch . . .	485	e−136
gi|3493013|gb|AAD02964.1|	(AF079244) granule-bound starch s . . .	483	e−135
gi|3493039|gb|AAD02977.1|	(AF079257) granule-bound starch s . . .	483	e−135
gi|3493015|gb|AAD02965.1|	(AF079245) granule-bound starch s . . .	483	e−135
gi|3493031|gb|AAD02973.1|	(AF079253) granule-bound starch s . . .	482	e−135
gi|3493001|gb|AAD02958.1|	(AF079238) granule-bound starch s . . .	481	e−135
gi|3493017|gb|AAD02966.1|	(AF079246) granule-bound starch s . . .	480	e−134
gi|3493009|gb|AAD02962.1|	(AF079242) granule-bound starch s . . .	479	e−134
gi|17432494|gb|AAL38185.1|	(AY062271) granule-bound starch . . .	479	e−134
gi|13377475|gb|AAK20726.1|	(AF318770) granule-bound starch . . .	479	e−134
gi|3493003|gb|AAD02959.1|	(AF079239) granule-bound starch s . . .	478	e−133
gi|3493033|gb|AAD02974.1|	(AF079254) granule-bound starch s . . .	478	e−133
gi|13774486|gb|AAK38882.1|	(AF353520) granule-bound starch . . .	477	e−133
gi|3492999|gb|AAD02957.1|	(AF079237) granule-bound starch s . . .	475	e−133
gi|3493101|gb|AAD03008.1|	(AF079288) granule-bound starch s . . .	475	e−133
gi|3493109|gb|AAD03012.1|	(AF079292) granule-bound starch s . . .	474	e−132
gi|3492997|gb|AAD02956.1|	(AF079236) granule-bound starch s . . .	474	e−132
gi|3493027|gb|AAD02971.1|	(AF079251) granule-bound starch s . . .	474	e−132
gi|3493029|gb|AAD02972.1|	(AF079252) granule-bound starch s . . .	468	e−131
gi|3493107|gb|AAD03011.1|	(AF079291) granule-bound starch s . . .	468	e−130
gi|3493117|gb|AAD03016.1|	(AF079296) granule-bound starch s . . .	464	e−129
gi|12278453|gb|AAG48967.1|	(AY010980) granule-bound starch . . .	462	e−129
gi|12278435|gb|AAG48958.1|	(AY010971) granule-bound starch . . .	461	e−129
gi|12278421|gb|AAG48951.1|	(AY010964) granule-bound starch . . .	461	e−129
gi|12278479|gb|AAG48980.1|	(AY010993) granule-bound starch . . .	461	e−129
gi|12278423|gb|AAG48952.1|	(AY010965) granule-bound starch . . .	461	e−128
gi|12278419|gb|AAG48950.1|	(AY010963) granule-bound starch . . .	461	e−128
gi|3493081|gb|AAD02998.1|	(AF079278) granule-bound starch s . . .	461	e−128
gi|3493071|gb|AAD02993.1|	(AF079273) granule-bound starch s . . .	460	e−128
gi|12278471|gb|AAG48976.1|	(AY010989) granule-bound starch . . .	460	e−128
gi|12278485|gb|AAG48983.1|	(AY010996) granule-bound starch . . .	460	e−128
gi|3493087|gb|AAD03001.1|	(AF079281) granule-bound starch s . . .	460	e−128
gi|12278437|gb|AAG48959.1|	(AY010972) granule-bound starch . . .	460	e−128
gi|12278449|gb|AAG48965.1|	(AY010978) granule-bound starch . . .	459	e−128
gi|12278495|gb|AAG48988.1|	(AY011001) granule-bound starch . . .	459	e−128
gi|12278491|gb|AAG48986.1|	(AY010999) granule-bound starch . . .	459	e−128
gi|13774484|gb|AAK38881.1|	(AF353519) granule-bound starch . . .	459	e−128
gi|3493083|gb|AAD02999.1|	(AF079279) granule-bound starch s . . .	459	e−128
gi|12278473|gb|AAG48977.1|	(AY010990) granule-bound starch . . .	459	e−128
gi|12278481|gb|AAG48981.1|	(AY010994) granule-bound starch . . .	459	e−128
gi|12278413|gb|AAG48947.1|	(AY010960) granule-bound starch . . .	459	e−128
gi|3493075|gb|AAD02995.1|	(AF079275) granule-bound starch s . . .	458	e−128
gi|12278429|gb|AAG48955.1|	(AY010968) granule-bound starch . . .	458	e−128
gi|12278477|gb|AAG48979.1|	(AY010992) granule-bound starch . . .	458	e−128
gi|12278463|gb|AAG48972.1|	(AY010985) granule-bound starch . . .	458	e−128
gi|12278427|gb|AAG48954.1|	(AY010967) granule-bound starch . . .	458	e−128
gi|12278469|gb|AAG48975.1|	(AY010988) granule-bound starch . . .	458	e−128
gi|12278411|gb|AAG48946.1|	(AY010959) granule-bound starch . . .	458	e−128
gi|13194734|gb|AAK15529.1|	(AF331953) granule-bound starch . . .	457	e−127
gi|12278493|gb|AAG48987.1|	(AY011000) granule-bound starch . . .	457	e−127
gi|12278431|gb|AAG48956.1|	(AY010969) granule-bound starch . . .	457	e−127
gi|12278433|gb|AAG48957.1|	(AY010970) granule-bound starch . . .	457	e−127
gi|12278497|gb|AAG48989.1|	(AY011002) granule-bound starch . . .	457	e−127
gi|12278425|gb|AAG48953.1|	(AY010966) granule-bound starch . . .	457	e−127
gi|12278443|gb|AAG48962.1|	(AY010975) granule-bound starch . . .	456	e−127
gi|12278507|gb|AAG48994.1|	(AY011007) granule-bound starch . . .	456	e−127
gi|13774480|gb|AAK38879.1|	(AF353517) granule-bound starch . . .	456	e−127
gi|12278489|gb|AAG48985.1|	(AY010998) granule-bound starch . . .	456	e−127
gi|3493095|gb|AAD03005.1|	(AF079285) granule-bound starch s . . .	456	e−127
gi|12278457|gb|AAG48969.1|	(AY010982) granule-bound starch . . .	455	e−127
gi|12278451|gb|AAG48966.1|	(AY010979) granule-bound starch . . .	455	e−127
gi|3493093|gb|AAD03004.1|	(AF079284) granule-bound starch s . . .	455	e−127
gi|3493085|gb|AAD03000.1|	(AF079280) granule-bound starch s . . .	455	e−127
gi|3493091|gb|AAD03003.1|	(AF079283) granule-bound starch s . . .	455	e−127
gi|13774482|gb|AAK38880.1|	(AF353518) granule-bound starch . . .	455	e−127
gi|3493097|gb|AAD03006.1|	(AF079286) granule-bound starch s . . .	454	e−127
gi|12278514|gb|AAG48997.1|	(AY011011) granule-bound starch . . .	454	e−126
gi|3493111|gb|AAD03013.1|	(AF079293) granule-bound starch s . . .	454	e−126
gi|3493051|gb|AAD02983.1|	(AF079263) granule-bound starch s . . .	454	e−126
gi|3493073|gb|AAD02994.1|	(AF079274) granule-bound starch s . . .	454	e−126
gi|3493113|gb|AAD03014.1|	(AF079294) granule-bound starch s . . .	454	e−126
gi|12278509|gb|AAG48995.1|	(AY011008) granule-bound starch . . .	454	e−126
gi|3493089|gb|AAD03002.1|	(AF079282) granule-bound starch s . . .	454	e−126
gi|12278503|gb|AAG48992.1|	(AY011005) granule-bound starch . . .	454	e−126
gi|3493115|gb|AAD03015.1|	(AF079295) granule-bound starch s . . .	454	e−126
gi|12278487|gb|AAG48984.1|	(AY010997) granule-bound starch . . .	454	e−126
gi|3493119|gb|AAD03017.1|	(AF079297) granule-bound starch s . . .	454	e−126
gi|3493077|gb|AAD02996.1|	(AF079276) granule-bound starch s . . .	454	e−126
gi|3493103|gb|AAD03009.1|	(AF079289) granule-bound starch s . . .	453	e−126
gi|3493099|gb|AAD03007.1|	(AF079287) granule-bound starch s . . .	453	e−126
gi|3493079|gb|AAD02997.1|	(AF079277) granule-bound starch s . . .	453	e−126
gi|12278417|gb|AAG48949.1|	(AY010962) granule-bound starch . . .	452	e−126
gi|3493065|gb|AAD02990.1|	(AF079270) granule-bound starch s . . .	452	e−126
gi|3493067|gb|AAD02991.1|	(AF079271) granule-bound starch s . . .	452	e−126
gi|12278511|gb|AAG48996.1|	(AY011009) granule-bound starch . . .	452	e−126
gi|12278505|gb|AAG48993.1|	(AY011006) granule-bound starch . . .	452	e−126
gi|3493049|gb|AAD02982.1|	(AF079262) granule-bound starch s . . .	452	e−126
gi|12278501|gb|AAG48991.1|	(AY011004) granule-bound starch . . .	451	e−126
gi|12278475|gb|AAG48978.1|	(AY010991) granule-bound starch . . .	451	e−125
gi|12278499|gb|AAG48990.1|	(AY011003) granule-bound starch . . .	451	e−125
gi|3493055|gb|AAD02985.1|	(AF079265) granule-bound starch s . . .	450	e−125
gi|12278415|gb|AAG48948.1|	(AY010961) granule-bound starch . . .	449	e−125
gi|3493069|gb|AAD02992.1|	(AF079272) granule-bound starch s . . .	448	e−125
gi|13774488|gb|AAK38883.1|	(AF353521) granule-bound starch . . .	448	e−125
gi|3493059|gb|AAD02987.1|	(AF079267) granule-bound starch s . . .	447	e−124
gi|3493057|gb|AAD02986.1|	(AF079266) granule-bound starch s . . .	446	e−124
gi|3493053|gb|AAD02984.1|	(AF079264) granule-bound starch s . . .	445	e−124
gi|12278516|gb|AAG48998.1|	(AY011012) granule-bound starch . . .	444	e−123
gi|3493105|gb|AAD03010.1|	(AF079290) granule-bound starch s . . .	443	e−123
gi|3493121|gb|AAD03018.1|	(AF079298) granule-bound starch s . . .	440	e−122
gi|3493063|gb|AAD02989.1|	(AF079269) granule-bound starch s . . .	440	e−122
gi|3493061|gb|AAD02988.1|	(AF079268) granule-bound starch s . . .	419	e−116
gi|17940636|gb|AAL49705.1|	(AF445166) granule-bound starch . . .	405	e−112
gi|17940634|gb|AAL49704.1|	(AF445165) granule-bound starch . . .	404	e−111
gi|17940622|gb|AAL49698.1|	(AF445159) granule-bound starch . . .	404	e−111
gi|13375389|gb|AAK20309.1|	(AF329738) granule bound starch . . .	404	e−111
gi|17940642|gb|AAL49707.1|	(AF445170) granule-bound starch . . .	404	e−111
gi|17940630|gb|AAL49702.1|	(AF445163) granule-bound starch . . .	404	e−111
gi|17940626|gb|AAL49700.1|	(AF445161) granule-bound starch . . .	403	e−111
gi|17940659|gb|AAL49715.1|	(AF445179) granule-bound starch . . .	403	e−111
gi|17940644|gb|AAL49708.1|	(AF445171) granule-bound starch . . .	403	e−111
gi|17940651|gb|AAL49711.1|	(AF445175) granule-bound starch . . .	402	e−111
gi|13375385|gb|AAK20307.1|	(AF329736) granule bound starch . . .	402	e−111
gi|17940677|gb|AAL49723.1|	(AF445189) granule-bound starch . . .	402	e−111
gi|13375387|gb|AAK20308.1|	(AF329737) granule bound starch . . .	402	e−111
gi|17940655|gb|AAL49713.1|	(AF445177) granule-bound starch . . .	402	e−111
gi|17940667|gb|AAL49719.1|	(AF445183) granule-bound starch . . .	402	e−111
gi|13375399|gb|AAK20314.1|	(AF329743) granule bound starch . . .	402	e−111
gi|17940628|gb|AAL49701.1|	(AF445162) granule-bound starch . . .	401	e−111
gi|17940639|gb|AAL49706.1|	(AF445168) granule-bound starch . . .	401	e−110
gi|17940679|gb|AAL49724.1|	(AF445190) granule-bound starch . . .	401	e−110
gi|13375381|gb|AAK20305.1|	(AF329734) granule bound starch . . .	400	e−110
gi|17940675|gb|AAL49722.1|	(AF445188) granule-bound starch . . .	400	e−110
gi|17940624|gb|AAL49699.1|	(AF445160) granule-bound starch . . .	400	e−110
gi|17940648|gb|AAL49710.1|	(AF445173) granule-bound starch . . .	400	e−110
gi|17940620|gb|AAL49697.1|	(AF445158) granule-bound starch . . .	400	e−110
gi|17940672|gb|AAL49721.1|	(AF445186) granule-bound starch . . .	400	e−110
gi|17940653|gb|AAL49712.1|	(AF445176) granule-bound starch . . .	399	e−110
gi|17940632|gb|AAL49703.1|	(AF445164) granule-bound starch . . .	399	e−110
gi|17940661|gb|AAL49716.1|	(AF445180) granule-bound starch . . .	398	e−110
gi|15983795|gb|AAL10494.1|	(AY056803) At1g32900/F9L11_8 [Ar . . .	386	e−106
gi|553108|gb|AAA33918.1|	(M55039) UDP-glucose starch glycos . . .	379	e−104
gi|13375405|gb|AAK20317.1|	(AF329746) granule-bound starch . . .	378	e−103
gi|8778105|gb|AAF79205.1|AF267643_1	(AF267643) starch synth . . .	377	e−103
gi|6116748|dbj|BAA85761.1|	(AB028026) granule-bound starch . . .	377	e−103
gi|1136128|gb|AAA84384.1|	(U41446) starch synthase [Sorghum . . .	355	7e−97
gi|7433860|pir||T07802	ADPglucose - starch glucosyltransfera . . .	329	5e−89
gi|7433861|pir||T07804	ADPglucose - starch glucosyltransfera . . .	328	1e−88
gi|2829792|sp|P93568|UGS2_SOLTU	Soluble glycogen [starch] s . . .	320	4e−86
gi|2833384|sp|Q43093|UGS3_PEA	Glycogen [starch] synthase, c . . .	314	2e−84
gi|2129898|pir||S61505	UDPglucose - starch glucosyltransfera . . .	314	2e−84
gi|7489695|pir||T06798	probable starch synthase (EC 2.4.1. - . . .	313	2e−84
gi|8708896|gb|AAC17970.2|	(AF026421) soluble starch synthas . . .	312	5e−84
gi|9369336|emb|CAB99210.1|	(AJ292522) starch synthase I-2 [. . .	311	2e−83
gi|6103327|gb|AAF03557.1|	(AF091802) starch synthase I [Aeg . . .	311	2e−83
gi|15237934|ref|NP_197818.1|	(NM_122336) soluble starch syn . . .	310	2e−83
gi|5880466|gb|AAD54661.1|	(AF091803) starch synthase I [Tri . . .	310	2e−83
gi|9369334|emb|CAB99209.1|	(AJ292521) starch synthase I-1 [. . .	310	3e−83
gi|7188796|gb|AAF37876.1|AF234163_1	(AF234163) starch synth . . .	310	4e−83
gi|15232051|ref|NP_186767.1|	(NM_110984) putative glycogen . . .	309	6e−83
gi|6690399|gb|AAF24126.1|AF121673_1	(AF121673) soluble star . . .	308	1e−82
gi|7489710|pir||T01208	ADPglucose - starch glucosyltransfera . . .	308	1e−82
gi|3192881|gb|AAC19119.1|	(AF068834) starch synthase [Ipomo . . .	307	3e−82
gi|16265834|gb|AAL16661.1|AF419099_1	(AF419099) putative so . . .	304	2e−81
gi|2833387|sp|Q43654|UGS2_WHEAT	Soluble glycogen [starch] s . . .	302	5e−81
gi|7489712|pir||T01414	ADPglucose--starch glucosyltransfera . . .	300	2e−80
gi|2833377|sp|Q40739|UGS2_ORYSA	Soluble glycogen [starch] s . . .	300	3e−80
gi|1549232|dbj|BAA07396.1|	(D38221) SSS1 [Oryza sativa] >gi . . .	300	3e−80
gi|12019656|gb|AAD45815.2|	(AF168786) soluble starch syntha . . .	300	3e−80

[0384]

SSI
Amino acid sequence of maize Soluble Starch Synthase I (SSI)
Accession: AF036891
NID: g2828011
Mol. wt. (calc) = 67843    Residues = 622
1	M A T P S A V G A A C L L L A R A A W P A A V G D R A R P R	SEQ. ID. No. 267
31	R L Q R V L R R R C V A E L S R E G P A P R P L P P A L L A
61	P P L V P G F L A P P A E P T G E P A S T P P P V P D A G L
91	G D L G L E P E G I A E G S I D N T V V V A S E Q D S E I V
121	V G K E Q A R A K V T Q S I V F V T G E A S P Y A K S G G L
151	G D V C G S L P V A L A A R G H R V M V V M P R Y L N G T S
181	D K N Y A N A F Y T E K H I R I P C F G G E H E V T F F H E
211	Y R D S V D W V F V D H P S Y H R P G N L Y G D K F G A F G
241	D N Q F R Y T L L C Y A A C E A P L I L E L G G Y I Y G Q N
271	C M F V V N D W H A S L V P V L L A A K Y R P Y G V Y K D S
301	R S I L V I H N L A H Q G V E P A S T Y P D L G L P P E W Y
331	G A L E W V F P E W A R R H A L D K G E A V N F L K G A V V
361	T A D R I V T V S K G Y S W E V T T A E G G Q G L N E L L S
391	S R K S V L N G I V N G I D I N D W N P A T D K C I F C H Y
421	S V D D L S G K A K C K G A L Q K E L G L P I R P D V P L I
451	G F I G R L D Y Q K G I D L I Q L I I P D L M R E D V Q F V
481	M L G S G D P E L E D W M R S T E S I F K D K F R G W V G F
511	S V P V S H R I T A G C D I L L M P S R F E F C G L N Q L Y
541	A M Q Y G T V P V V H A T G G L R D T V E N F N P F G E N G
571	E Q G T G W A F A P L T T E N M F V D I A N C N I Y I Q G T
601	Q V L L G R A N EA R H V K R L H V G P C R

[0385]

TABLE XIII
Maize soluble starch synthase I (SSI)
Alignments with other similar proteins—Transit Peptide
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)            Sequence            ending	#
268	MAIZESSI	1	MATPSAVGAACLLLARAA-----WPAAVGDXXXXXXXXXXXXXXCVAELSREG--XXXXX	53
269	7489712	1	MATPSAVGAACLLLARAA-----WPAAVGDRARPRRLQRVLRRRCVAELSREG--PAPRP	53
270	12019656	1	MATPSAVGAACLVLARAAAGLGLGPGRGGDRARPRRFQRVVRRRCVAELSREGPAPTPRP	60
271	1549232	34	CVAELSRDG--GSAQR	47
272	2833377	34	CVAELSRDG--GSAHG	47
273	5295947	34	CVAELSRDG--GSAQR	47
268	MaizeSSI	54	XXXXXXXXXXXXXXXXXXXEPTGEPASTPPPVP--------------DAGLGDLG--LEP	97
269	7489712	54	LPPALLAPPLVPGFLAPPAEPTGEPASTPPPVP--------------DAGLGDLG--LEP	97
270	12019656	61	LPPALLAPPLVPAFLAPPSEPEGEPASTFPPLP--------------DAGLGDLG--LQP	104
271	1549232	48	PLAPAPLVKQPVL-------PTFLVPTSTPPAPTQSPAPAPTPPPLPDSGVGEIE--PDL	98
272	2833377	48	PLAPAPLVKQPVL-------PTFLVPTSTPPAPTQSPAPAPTPPPLPDSGVGEIE--PDL	98
273	5295947	48	PLAPAPLVKQPVL-------PTFLVPTSTPPAPTQSPAPAPTPPPLPDSGVGEIE--PDL	98
274	9369336	79	PAQSPAPTQPPLP--------------DAGVGELAPDLLL	104
275	6103327	79	PAQSPAPTQPPLP--------------DAGVGELAPDLLL	104
276	9369334	79	PAQSPAPTQPPLP--------------DAGVGELAPDLLL	104
277	5880466	79	PAQSPAPTQPPLP--------------DAGVGELAPDLLL	104
278	7188796	75	PAQSPAPTQPPLP--------------DAGVGELAPDLLL	100
279	16265834	281	EP	282
280	2833381	25	GLGQLA--LRS	33
281	297424	31	GFQG--LKP	37
282	82478	31	GFQG--LKP	37
283	2833382	31	GFQG--LKP	37

[0386]

TABLE XIV
Maize soluble starch synthase I (SSI)
“GLASS” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)            Sequence            ending	#
284	MaizeSSI	98	EGIAEG-----SID--NTVVVASEQD--SEIVVGKEQA--R-----AKVT----------	131
285	7489712	98	EGIAEG-----SID--NTVVVASEQD--SEIVVCKEQA--R-----AKVT----------	131
287	12019656	105	EGIAEG-----SID--ETVVVASEQD--SEIVVGKEQA--R-----AKVT----------	138
288	1549232	99	EGLTED-----SID--KTIFVASEQE--SEIMDVKEQA--Q-----AKVT----------	132
289	2833377	99	EGLTED-----SID--KTIFVASEQE--SEIMDVKEQA--Q-----AKVT----------	132
290	5295947	99	EGLTED-----SID--KTIFVASEQE--SEIMDVKEQA--Q-----AKVT----------	132
291	9369336	105	EGIAED-----SID--SIIVAASEQD--SEIMDAKDQP--Q-----AKVT----------	138
292	6103327	105	EGIAED-----SID--SIIVAASEQD--SEIMDANEQP--Q-----AKVT----------	138
293	9369334	105	EGIAED-----SID--SIIVAASEQD--SEIMDANEQP--Q-----AKVT----------	138
294	5880466	105	EGIAED-----SID--SIIVAASEQD--SEIMDANEQP--Q-----AKVT----------	138
295	7188796	101	EGIAED-----SID--TIVVAASEQD--SEIMDANDQP--L-----AKVT----------	134
296	15237934	131	VGIPSG--K-----AEVV----------	141
297	6690399	54	VGIPSG--K-----AEVV----------	64
298	2829792	102	VE--SHDIVANDRDDLSEDTEEMEET--P-----IKLT----------	130
299	15232051	268	EK-----YVE--KTPDVASSET--NE--PGKDEE--KPPPLAGANV----------	300
300	8708896	65	AEEEE--PPAVEKPPPL--A-----GPNV----------	84
301	15384987	126	VSSADD--SENKESGPLA--G-----PNVM----------	146
302	15028467	183	VSSADD--SENKESGPLA--G-----PNVM----------	203
303	16265834	283	DAAEDG-----DDD--DDWADSDASD--SEIDQDDDSGPLA-----GENV----------	318
304	5441242	68	GREKV--L-----EKIECG--------	79
305	12003285	54	--NNVKVSAKRV--GQVPINKDRC--E-----TS-G----------	77
306	2833381	34	QAVTHN-----GLRPVNKIDMLQLRT--SAPNLAKMEG--K-----MRVEWQAGTIVCKQ	79
307	297424	38	RSPAGGDATSLSVT--TSARATPKQQ--RSVQRGSRRF--P-----SVVVYATGAG----	82
308	82478	38	RSPAGGDATSLSVT--TSARATPKQQ--RSVQRGSRRF--P-----SVVVYATGAG----	82
309	2833385	78	-----ATAG----------	81
310	2833382	38	RSPAGGDATSLSVT--TSARATPKQQ--RSVQRGSRRF--P-----SVVVYATGAG----	82
284	(maize SSI)	132	--QSIVFVTGEASPYAKSGGLGDVCGSLPVALAARGHRVMVVMPRYLN-GT-SDKNYANA	187
285	7489712	132	--QSIVFVTGEASPYAKSGGLGDVCGSLPVALAARGHRVMVVMPRYLN-GT-SDKNYANA	187
286	12019656	139	--QSIVFVTGEASPYAKSGGLGDVCGSLPVALAARGHRVMVVMPRYLN-GT-SDKNYANA	194
287	1549232	133	--RSVVFVTGEASPYAKSGGLGDVCGSLPIALALRGNRVMVVMPRYMN-GA-LNKNFANA	188
288	2833377	133	--RSVVFVTGEASPYAKSGGLGDVCGSLPIALAIRGHRVMVVMPRYMN-GA-LNKNFANA	188
289	5295947	133	--RSVVFVTGEASPYAKSGGLGDVCGSLPIALALRGHRVMVVMPRYMN-GA-LNKNFANA	188
290	9369336	139	--RSIVFVTGEAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLN-GS-SDKNYAKA	194
291	6103327	139	--RSIVFVTGEAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLN-GS-SDKNYAKA	194
292	9369334	139	--RSIVFVTGEAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLN-GS-SDKNYAKA	194
293	5880466	139	--RSIVFVTGEAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLN-GS-SDKNYAKA	194
294	7188796	135	--RSIVFVTGEAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLN-GT-SDKNYAKA	190
295	2833387	1	--        EAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLN-GS-SDKNYAKA	48
296	15237934	142	--NNLVFVTSEAAPYSKTGGLGDVCGSLPIALAGRGHRVMVISPRYLN-GTAADKNYARA	198
297	6690399	65	--NNLVFVTSEAAPYSKTGGLGDVCGSLPIALAGRGHRVMVISPRYLN-GTAADKNYARA	121
298	2829792	131	--FNIIFVTAEAAPYSKTGGLGDVCGSLPMALAARGHRVMVVSPRYLNGGP-SDEKYANA	187
299	15232051	301	--MNVILVAAECAPFSKTGGLGDVAGALPKSLARRGHRVMVVVPRY--------AEYAEA	350
311	3192881	140	NVILVCAECAPWSKTGGLGDVAGALPKALARRGHRVMVVVPLY--------GNYAEP	188
310	2833384	262	NIILVSAECAPWSKTCGLGDVAGSLPKALARRGHRVMIVAPHY--------GNYAEA	310
312	2129898	262	NIILVSAECAPWSKTGGLGDVAGSLPKALARRGHRVMIVAPHY--------GNYAEA	310
313	6467503	261	NVILVAAECAPWSKTGGLGDVAGSLPKALAPRGHRVMVVAPRY--------GNYVEP	309
314	14495348	259	NIILVAAECAPWSKTGGLGDVAGALPKALARRGHRVMVVVPMY--------KNYAEP	307
315	7489711	208	NVVVVASECAPFCKTGGLGDVVGALPKALARRGHRVMVVIPRY--------GEYAEA	256
300	8708896	85	--NNVVMVGAECAPWSKTGGLGDVMAALPKALVRRGHRVMVVVPRY--------ENYDNA	134
301	15384987	147	--NVIV-VASECSPFCKTGGLGDVVGALPKALARRGHRVMVVIPRY--------GEYAEA	195
302	15028467	204	--NVIV-VASECSPFCKTGGLGDVVGALPKALARRGHRVMVVIPRY--------GEYAEA	252
316	7489710	242	NVIVVAAECSPWCKTGGLGDVVGALPKALARRGHRVMVVVPRY--------GDYVEA	290
317	2833390	297	NIILVASECAPWSKTGGLGDVAGALPKALARRGHRVMVVAPRYDN-YP-EPQDSG--	349
318	7489695	1	NVVVVAAECSPWCKTGGLGDVAGALPKALAKRGHRVMVVVPRY--------GDYEEA	49
319	8953573	308	NVVVVAAECSPWCKTGGLGDVAGALPKALAKRGHRVMVVVPRY--------GDYEEA	356
320	8953571	309	NVVVVAAECSPWCKTGGLGDVAGALPKALAKRGHRVMVVVPRY--------GDYEEA	357
321	7529653	309	NVVVVAAECSPWCKTGGLGDVAGALPKALAKRGHRVMVVVPRY--------GDYEEA	357
303	16265834	319	--MNVIVVAAECSPWCKTGGLGDVAGALPKALARRGHRVMVVVPRY--------GDYAEA	368
322	5825480	309	NVVVVAAECSPWCKTGGLGDVAGALPKALAKRGHRVMVVVPRY--------GDYEEA	357
323	2833388	83	NLIFVGAEVGPWSKTGGLGDVLGGLPRAMAARGHRVMTVSPRY--------DQYKDA	131
304	5441242	80	--MNLIFVGAEVAPWSKTGGLGDVLGGLPSALAEHGHRVMTVSPRY--------DQYKDA	129
305	12003285	78	--MTLIFVSAECGPWSKTGGLGDVVGGLPPALAANRHRVMTVSPRY--------DQYKDA	127
324	267196	82	NLIFVGTEVGPWSKTGGLGDVLGGLPPALAARGHRVMTISPRY--------DQYKDA	130
325	228210	82	NLIFVGTEVGPWSKTGGLGDVLGGLPPALAARGHRVMTISPRY--------DQYKDA	130
326	602594	82	NLIFVGTEVGPWSKTGGLGDVLGGLPPALAARGURVMTISPRY--------DQYKDA	130
327	16716335	61	IVMVAAEVAPWSKTGGLGDVTGGLPIELVKRGHRVMTIAPRY--------DQYADA	108
328	6136121	83	NLVFVLAEVGPWSKTGGLGDVVGGLPPAMAGNGHRVMTVSPRY--------DQYKDA	131
329	15223331	85	SVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTICPRY--------DQYKDA	133
330	15626365	88	NLIFVGTEVAPWSKTGGLGDVLGGLPPALSANGHRVMTVTPRY--------DQYKDA	136
331	3832512	82	NLVFVGAEVGPWSKTGGLGDVLGGLPPALAGNGHRVMTVSPRY--------DQYKDA	130
332	2833381	80	QGMNLVFVGCEEGPWCKTGGLGDVLGGLPPAIAARGHRVMTVCPRY--------DQYKDA	131
333	15637079	83	NLVFVGCEVGPWCKTGGLGDVLGGLPPALAARGHRVMTVCPRY--------DQYKDA	131
334	2833383	78	SLVFVGAEVGPWSKTGGLGDVLGGLPPVLAGNGHRVMTVSPRY--------DQYKDA	126
307	297424	83	--MNVVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVISPRY--------DQYKDA	132
335	82478	83	--MNVVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVISPRY--------DQYKDA	132
309	2833385	82	--MNVVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVVSPRY--------DQYKDA	131
336	2833382	83	--MNVVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVISPRY--------DQYKDA	132
284	(maize SSI)	188	FYTEKHIRIPCFGGEHE-VTFFHEYRDSVDWVFV-DHPSY------H-R-P------G-N	230
285	7489712	188	FYTEKHIRIPCFGGEHE-VTFFHEYRDSVDWVFV-DHPSY------N-R-P------G-N	230
286	12019656	195	FYTEKHIRIFCFGGEHE-VTFFHEYRDSVDWVFV-DHPSY------H-R-P------G-N	237
287	1549232	189	FYTEKHIKIPCFGGEHE-VTFFHEYRDSVDWVFV-DHPSY------H-R-P------G-N	231
288	2833377	189	FYTEKHIKIPCFGGEHE-VTFFHEYRDSVDWVFV-DHPSY------H-R-P------G-N	231
289	5295947	189	FYTEKHIKIPCFGGEHE-VTFFHEYRDSVDWVFV-DHPSY------H-R-P------G-N	231
290	9369336	195	LYTAKHIKIPCFGGSHE-VTFFHEYRDNVDNVFV-DHPSY------H-R-P------G-S	237
292	6103327	195	LYTAKHIKIPCFGGSHE-VTFFHEYRDNVDWVFV-DHPSY------H-R-P------G-S	237
292	9369334	195	LYTGKHIKIPCFGGSHE-VTFFHEYRDNVDWVFV-DHPSY------H-R-P------G-S	237
293	5880466	195	LYTGKHIKIPCFGGSHE-VTFFHEYRDNVDWVFV-DHPSY------H-R-P------G-S	237
294	7188796	191	LYTGKUIKIPCFGGSHE-VTFFHEYRDNVDWVFV-DHPSY------H-R-P------G-S	233
295	2833387	49	LYTAKHIKIPCFGGSHE-VTFFHEYRDNVDWVFV-DHPSY------H-R-P------G-S	91
296	15237934	199	KDLGIRVTVNCFGGSQE-VSFYHEYRDGVDWVFV-DHKSY------H-R-P------G-N	241
297	6690399	122	KDLGIRVTVNCFGGSQE-VSFYHEHRDGVDWVFV-DHKSY------H-R-P------G-N	164
298	2829792	188	VDLDVRAIVHCFGDAQE-VAFYHEYRAGVDWVFV-DHSSY------C-R-P------G-T	230
299	15232051	351	KDLGVRKRYKVAGQDME-VMYFHAFIDGVDFVFI-DSPEFR-----H-L-S------N-N	394
311	3192881	189	QHTGVRKMFKIDGQDME-VNYFHAYIDNVDFVFI-DSPIFQ-----H-R-G------N-N	232
310	2833384	311	HDIGVRKRYKVAGQDME-VTYFHTYIDGVDIVFI-DSPIF------R-NLE------S-N	354
312	2129898	311	HDIGVRKRYKVAGQDME-VTYFHTYIDGVDIVFI-DSPIF------R-NLE------S-N	354
313	6467503	310	QDTGVRKRYKVDGQDFE-VSYFQAFIDGVDFVFI-DSPMF------R-H-I------G-N	352
314	14495348	308	QQLGEPRRYQVAGQDME-VIYYHAYIDGVDFVFI-DNPIF------H-H-V------E-N	350
315	7489711	257	RDLGVRRRYKVAGQDSE-VTYFHSYIDGVDFVFV-EAPPFR-----H-R-H------N-N	300
300	8708896	135	WETGIRKIYSVFNSNQE-VGYFHAFVDGVDYVFV-DHPTF------HGR-G------K-N	178
301	15384987	196	KDLGVRKRYRVAGQDSE-VSYFHAFIDGVDFVFL-EAPPFR-----H-R-H------N-D	239
302	15028467	253	KDLGVRKRYRVAGQDSE-VSYFHAFIDGVDFVFL-EAPPFR-----H-R-H------N-D	296
316	7489710	291	FDMGIRKYYKAAGQDLE-VNYFHAFIDGVDFVFI-DAPLFR-----H-R-Q------D-D	334
317	2833390	350	--VRKIYKVD--GQDVD-VTYFQALLMDCDFVFIHSHMFR------H-I-G------N-N	389
318	7489695	50	YDVGVRKYYKAAGQDME-VNYFHAYIDGVDFVFI-DAPLFR-----H-R-Q------E-D	93
320	8953573	357	YDVGVRKYYKAAGQDME-VNYFHAYIDGVDFVFI-DAPLFR-----H-R-Q------E-D	400
320	8953571	358	YDVGVRKYYKAAGQDME-VNYFHAYIDGVDFVFI-DAPLFR-----H-R-Q------E-D	401
321	7529653	358	YDVGVRKYYKAAGQDME-VNYFHAYIDGVDFVFI-DAPLFR-----H-R-Q------E-D	401
303	16265834	369	QDVGIRKYYKAAGQDLE-VKYFHAFIDGVDFVFI-DAPLFR-----H-R-Q------D-D	412
322	5825480	358	YDVGVRKYYKAAGQDME-VNYFHAYIDGVDFVFI-DAPLFR-----H-R-Q------E-D	401
323	2833388	132	WDTSVSVEIKIGDRIET-VRFFHSYKRGVDRVFV-DHPMF------L-E-KVWGKTGS-K	180
304	5441242	130	WDTNVTVEVKVADRIET-VRFFHCYKQGVDRVFV-DHPCF------L-E-KVWGKTGS-K	178
305	12003285	128	WDTSVVVEIQVGDKVET-VGFFHCYKRGVDRVFV-DHPLFLEKVWGK-T-K------S-K	176
324	267196	131	WDTSVAVEVKVGDSIEI-VRFFHCYKRGVDRVFV-DHPMF------L-E-KVWGKTGS-K	179
325	228210	131	WDTSVAVEVKVGDSIEI-VRFFHCYKRGVDRVFV-DHPMF------L-E-KVWGKTGS-K	179
326	602594	131	WDTSVAVEVKVGDSIEI-VRFFHCYKRGVDRVFV-DHPMF------L-E-KVWGKTGS-K	179
327	16716335	109	WDTSVVVDIM---GE-K-VRYFHSIKKGVHRVWI-DHPWFLAKVWGK-T-G------S-K	153
328	6136121	132	WDTSVVVEIKVGDSIET-VRFFHCYKRGVDRVFV-DHPIFLEKVWGK-T-K------S-K	180
329	15223331	134	WDTCVVVQIKVGDKVEN-VRFFHCYKRGVDRVFV-DHPIFLAKVVGK-T-G------S-K	182
330	15626365	137	WDTNVTIEVKVGDRTEK-VRFFHCFKRGVDRVFV-DHPIF------L-E-KVWGKTGT-K	185
331	3832512	131	WDTGVSVEIKVGDRFET-VRFFHCYKRGVDRVFV-DHPLFLEKVWGK-T-E------S-K	179
332	2833381	132	WETCVVVE-PQVGDRIEPVRFFHSYKRGVDRVFV-DHPMFLEKVW-G-K-T------G-S	179
333	15637079	132	WDTCVVVELQVGDRIEP-VRFFHSYKRGVDRVFV-DHPMFLEKVE-G-K-T------G-S	179
334	2833383	127	WDTNVLVEVKVGDKIET-VRFFHCYKRGVDRVFV-DHPLFLERVWGK-T-G------S-K	175
307	297424	133	WDTSVVAEIKVADRYER-VRFFHCYKRGVDRVFI-DHPSFLEKVW-G-K-T------GEK	181
335	82478	133	WDTSVVAEVKVADRYER-VRFFHCYKRGVDRVFI-DHPSFLEKVW-G-K-T------GEK	181
309	2833385	132	WDTSVVSEIKMGDGYET-VRFFHCYKRGVDRVFI-DHPLFLERVWGK-T-E------E-K	180
310	2833382	133	WDTSVVAEIKVADRYER-VRFFRCYKRGVDRVFI-DHPSFLEKVW-G-K-T------GEK	181
284	(Maize SSI)	231	-LYGDKFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-NC	271
285	7489712	231	-LYGDKFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-NC	271
286	12019656	238	-LYGDKFG-A-FGDNQFRYTLLCYAACEAPLVLEL--GG--Y-----I-----YG-Q-NC	278
287	1549232	232	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-KC	272
288	2833377	232	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-KC	272
289	5295947	232	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-KC	272
290	9369336	238	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-NC	278
291	6103327	238	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-NC	278
292	9369334	238	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-NC	278
293	5880466	238	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-NC	278
294	7188796	234	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-SC	274
295	2833387	92	-LYGDNFG-A-FGDNQFRYTLLCYAACEAPLILEL--GG--Y-----I-----YG-Q-NC	132
296	15237934	242	-PYGDSKG-A-FGDNQFRFTLLCHAACEAPLVLPL--GG--F-----T-----YG-E-KS	282
297	6690399	165	-PYGDSKG-A-FGDNQFRFTLLCHAACEAPLVLPL--GG--F-----T-----YG-E-KS	205
298	2829792	231	-PYGDIYG-A-FGDNQFRFTLLSHAACEAPLVLPL--GG--F-----T-----YG-E-KC	271
299	15232051	395	-IYG---G-N-RLDILKRMVLFCKAAVEVPWYVPC--GG--V-----C-----YG-DGNL	433
311	3192881	233	-IYG---G-N-RVDILKRMDLFCKAAIVVPWHVPC--GG--I-----C-----YG-DGNL	271
310	2833384	355	-IYG---G-N-RLDILRRMVLFCKAAVEVPWHVPC--GG--I-----C-----YG-DGNL	393
312	2129898	355	-IYG---G-N-RLDILRRMVLFCKAAVEVPWHVPC--GG--I-----C-----YG-DGNL	393
313	6467503	353	DIYG---G-N-RMDILKRMVLFCKAAVEVPWHVPC--GG--V-----C-----YG-DGNL	392
314	14495348	351	DIYG---G-D-RTDILKRMVLLCKAAIEVPWYVPC--GG--Y-----C-----YG-DGNL	390
315	7489711	301	-IYG---G-E-RLDILKRMILFCKAAVEVPWYAPC--GG--T-----V-----YG-DGNL	339
300	8708896	179	-IYG---G-E-RQEILFRCALLCKAALEAVWHVPC--GG--I-----T-----YGDD-NL	217
301	15384987	240	-IYG---G-E-RFDVLKRMILFCKAAVEVPWFAPC--GG--S-----I-----YG-DGNL	278
302	15028467	297	-IYG---G-E-RFDVLKRMILFCKAAVEVPWFAPC--GG--S-----I-----YG-DGNL	335
316	7489710	335	-IYG---G-S-RQEIMKRMILFCKVAVEVPWHVPC--GG--V-----C-----YG-DGNL	373
317	2833390	390	-IYG---G-N-RVDILKRMVLFCKAAIEVPWHVPC--GG--V-----C-----YG-DGNL	428
318	7489695	94	-IYG---G-S-RQEIMKRMVLFCIAVEVPVWHVPC--GG--V-----P-----YG-DGNL	132
319	8953573	401	-IYG---G-S-RQEIMKPMILFCKAAVEVPWHVPC--GG--V-----P-----YG-DGNL	439
320	8953571	402	-IYG---G-S-RQEIMKRMILFCKAAVEVPWHVPC--GG--V-----P-----YG-DGNL	440
321	7529653	402	-IYG---G-S-RQEIMKRMILFCKAAVEVPWHVPC--GG--V-----P-----YG-DGNL	440
303	16265834	413	-IYG---G-N-RQEIMKRMILFCKAAVEVPWHVPC--GG--V-----P-----YG-DGNL	451
322	5825480	402	-IYG---G-S-RQEIMKPMILFCKAAVEVPWHVPC--GG--V-----F-----YG-DGNL	440
323	2833388	181	-IYGPFAG-LDYQDNQLRFSLLCLAALEAPRVLNL--NS--S-----KNFSGPYG-E-EV	227
304	5441242	179	-LYGPSAG-VDYEDNQLRYSLLCQAALEAPRVLNL--NSNKY-----FSGP--YG-E-DV	225
305	12003285	177	-VYGPSAG-VDYEDNQLRFSLLSLAALEAFRVLNL--TSNKY-----FSGP--YG-E-DV	223
324	267196	180	-IYGPKAG-LDYLDNELRFSLLCQAALEAPKVLNLNSSN--Y-----FSGP--YG-E-DV	226
325	228210	180	-IYGPKAG-LDYLDNELRFSLLCQAALEAPKVLNLNSSN--Y-----FSGP--YG-E-DV	226
326	602594	180	-IYGPKAG-LDYLDNELRFSLLCQAALEAPKVLNLNSSN--Y-----FSGP--YG-E-DV	226
327	16716335	154	-LYGPRSG-ADYLDNHKRFALFCKAAIEAARVLPF--GP---------------G-E-DC	192
328	6136121	181	-IYGPNAG-TDYQDNQLRFSLLCQAALEAPRVLNLTSSK--Y-----FSGP--YG-E-DV	227
329	15223331	183	-IYGPITG-VDYNDNQLRFSLLCQAALEAPQVLNL--NSSKY-----FSGF--YG-E-DV	229
330	15626365	186	-LYGPAAGDD-YQDNQLRFSIFCQAALEAARVLNL--KSNKY-----FSGP--YG-E-DV	232
331	3832512	180	-LYGPKTG-VDYKDNQLRFSLLCQAALEAPRVLNL--NS--N-----KHFSGPYG-E-DV	226
332	2833381	180	MLYGPKAG-KDYKDNQLRFSLLCQAALEAPRVLNLNSSN--Y-----FSGP--YG-E-DV	227
333	15637079	180	MLYGPKAG-KDYKDNQLRFSLLCQAALEAPRVLNLNSSN--Y-----FSGP--YG-E-DV	227
334	2833383	176	-LYGPKTG-IDYRDNQLRFSLLCQAALEAPRVLNL--NSSKY-----FSGP--YG-E-DV	222
307	297424	182	-IYGPDTG-VDYKDNQMRFSLLCQAALEAPRILNL--NN--NPYFKGT-----YG-E-DV	228
335	82478	182	-IYGPDTG-VDYKDNQMRFSLLCQAALEAPRILNL--NN--NPYFKGT-----YG-E-DV	228
309	2833385	181	-KYGPDAG-TDYKDNQLRFSLLCQAALEAPRILSL--NNNPY-----FSGP--YG-E-DV	227
310	2833382	182	-IYGPDTG-VDYKDNQMRFSLLCQAALEAPRILNL--NN--NPYFKGT-----YG-E-DV	228
284	(maize 881)	272	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYG	331
285	7489712	272	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEQYG	331
286	12019656	279	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEQYG	338
287	1549232	273	MFVVNDWHASLVPVLLAAKYRPYGVYPDSRSILVIHNLAHQGVEPASTYPDLGLPPEQYG	332
288	2833377	273	MFVVNDWHASLVPVLLAAKYRPYGVYRDSRSILVIHNLAHQGVEPASTYPDLGLPPEQYG	332
289	5295947	273	MFVVNDWHASLVPVLLAAKYRPYGVYRDSRSILVIHNLAHQGVEPASTYPDLGLPPEQYG	332
290	9369336	279	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYG	338
291	6103327	279	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYG	338
292	9369334	279	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYG	338
293	5880466	279	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYG	338
294	7188796	275	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYG	334
295	2833387	133	MFVVNDWHASLVPVLLAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYG	192
296	15237934	283	LFLVNDWHAGLVPILLAAKYRPYGVYKDARSILIIHNLAHQGVEPAATYTNLGLPSEWYG	342
297	6690399	206	LFLVNDWHAGLVPILLAAKYRPYGVYKDARSILIIHNLAHQGVEPAATYTNLGLPSEWYG	265
298	2829792	272	LFLANDWHAALVPLLLAAKYRPYGVYKDARSIVAIHNIAHQGVEPAVTYNNLGLPPQWYG	331
299	15232051	434	AFIANDWHTALLPVYLKAYYPRDHGIMDYTRSVLVIHNIAHQGRGPVDFSYVDLPSHYLD	493
311	3192881	272	VFIANDWHTALLPVYLKAYFRDNGVMKFTRSVLVIHNIAHQGRGPMDDFSIVDLPAQYAD	331
310	2833384	394	VFIABDWHTALLPVYLKAYYRDHGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLD	453
312	2129898	394	VFIABDWHTALLPVYLKAYYRDHGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLD	453
313	6467503	393	AFIANDWHTALLPVYLKAYYRDNGLMQYTRSVLVIHNIAHQGRGPSGDFSYVGLPEHYI-	451
314	14495348	391	VFLANDWHTALLPVYLKAYYHDNGFMIYARSVLVIHNIAHQGRGPLDDFSYLDLPVDYMD	450
315	7489711	340	VFIANDWHTALLPVYLKAYYRDNGLMQYARSVLVIHNIAHQGRGPVDDFVNFDLPEHYID	399
300	8708896	218	CFIANDWHTALLPVYLQAHYRDYGEMTYARCAFVIHNMAHQGRGPFVESEHLELNEE---	274
301	15384987	279	VFIANDWHTALLPVYLKAYYRDNGLMQYTRSVLVIHNIAHQGRGPVDDFATMDLPEHYID	338
302	15028467	336	VFIANDWHTALLPVCLKAYYRDNGLMQYTRSVLVIHNIAHQGRGPVDDFATMDLPEHYID	395
316	7489710	374	VFIANDWHTALLPVYLKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQ	433
317	2833390	429	VFIANDWHTALLPAYLKAYYRDNGIMNYTRSVLVIHNIAHQGRGPLEDFSYVDLPPHYMD	488
318	7489695	133	VFIANDWHTALLPVYLKAYYRDHGLMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYL-	191
319	8953573	440	VFIANDWHTALLPVYLKAYYPHGIJMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYL-	498
320	8953571	441	VFIANDWHTALLPVYLKAYYRDHGLMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYL-	499
321	7529653	441	VFIANDWNTALLPVYLKAYYRDHGLMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYL-	499
303	1626583	4452	VFLANDWHTALLPVYLKAYYRDNGMMQYTRSVILVIHNAYQGRGPVDEFPYMELPEHYLD	511
322	5825480	441	VFIANDWHTALLPVYLKAYYRDHGLMQYTRSIMVIHINAHQGRGPVDEFPFTELPEHYL-	499
323	2833388	228	AFIANDWHTALLPCYLKAIYQPMGIYKHAKVAFCIHNIAYQGRFAFSDFPRLNLPDKEFS	287
304	5441242	226	IFVANDWHTALLPCYLKSMYQTRGVYRNTKVAFCIHNISYQGRHPFEDFPLLNLPNEYRS	285
305	12003285	224	VFVANDWHTAVLPCYLKTIYQPKGIYTNAKVVLCIHNIAYQGRFAFSDFYKLNLPDQLKS	283
324	267196	227	LFIANDWHTALIFCYLKSMYQSRGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRG	286
325	228210	227	LFIANDWHTALIFCYLKSMYQSRGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRG	286
326	602594	227	LFIANDWHTALIFCYLKSMYQSRGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRG	286
327	16716335	193	VFVANDWHSALVPVLLKDEYQPKGQFTKAKSVLAIHNIAFQGRNWEEAFKDTKLPQAAFD	252
328	6136121	228	VFVANDWHTALLPCYLKSMYQSKGMYLHAKVAFCIHNIAYQGRFGSSDFCLLNLPDQFKS	287
329	15223331	230	VFVANDWNTALLPCYLKSMYQSRGVYMNAKVVFCIHNIAYQGRFAFDDYSLLNLPISFKS	289
330	15626365	233	IFVANDWHTALISCYMKSMYQSIGIFRNAKVVFCIHNIAYQGRFAFTDYSLLNLPDQFKS	292
331	3832512	227	VFVANDWHTALLPCYLKSLYKSKGIYKSAKVAFCIHNIAYQGRHAFSDLSLLNLPNEFRS	286
332	2833381	228	VFVANDWHTALLPCYLKTMYQSRGIYMNAKVAFCIHNIAYQGRFAFSDFSLLNLPDEYKG	287
333	15637079	228	VFVANDWHTALLPCYLKTMYQSRGIYMNAKVAFCIHNIAYQGRFAFSDFSLLNLPDEYKG	287
332	2833383	223	IFVANDWHSALIPCYLKSMYKSRGLYKNAKVAFCIHNIAYQGRNAFSDFSLLNLPDEFRS	282
307	297424	229	VFVCNDWHTGPLASYLKNNYQPNGIYFNAKVAFCIHNISYQGRFAFEDYPELNLSERFRS	288
335	82478	229	VFVCNDWHTGPLASYLKNNYQPNGIYPNAKVAFCIHNISYQGRFAFEDYPELNLSERFRS	288
309	2833385	228	VFVCNDWHTGPLSCYLKSNYQSNGIYKDAKTAFCIHNISYQGRFAFSDFPELNLPERFKS	287
310	2833382	229	VFVCNDWHTGPLASYLKSNYQPNGIYRNAKVAFCIHNISYQGRFAFEDYPELNLSERFRS	288

[0387]

TABLE XV
Maize soluble starch synthase I (SSI)
“LINKR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)            Sequence            ending	#
336	MaizeSSI	332	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSKGYSWE	375
337	7489712	332	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSKGYSWE	375
338	12019656	339	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSKGYSWE	382
339	1549232	333	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	376
340	2833377	333	ALEW------VFPEWARRRALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	376
341	5295947	333	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	376
342	9369336	339	ALEW------VFPEWARREALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	382
343	6103327	339	ALEW------VFPEWARRRALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	382
344	9369334	339	ALEW------VFPEWARPRALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	382
345	5880466	339	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	382
346	7188796	335	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	378
347	2833387	193	ALEW------VFPEWARRHALD---KG-------EAVNFLKGAVVTADRIVTVSQGYSWE	236
348	15237934	343	AVGW------VFPTWARTHALD---TG-------EAVNVLKGAIVTSDRIITVSQGYAWE	386
349	6690399	266	AVGW------VFPTWARTHALD---TG-------EAVNVLKGAIVTSDRIITVSQGYAWE	309
350	2829792	332	AVEW------IFPTWAPAHALD---TG-------ETVNVLKGAIAVADRILTVSQGYSWE	375
351	15232051	494	SFKL------YDPV-----------GG-------EHFNIFAAGLKAADRVLTVSHGYSWE	529
352	3192881	332	LFKL------YDPV-----------GG-------DHFNIFAAGLKTADRVVTVSHGYAWE	367
353	2833384	454	LFKM------YDPV-----------GG-------EHFNIFAAGLKTADRIVTVSHGYAWE	489
354	2129898	454	LFKM------YDPV-----------GG-------EHFNIFAAGLKTADRIVTVSHGYAWE	489
355	6467503	452	-------------DLFKLHDFI---GG-------DHFNIFAPGLKVADRVVTVSHGYAWE	488
356	14495348	451	LFKL------YDPF-----------GG-------DHLNIFAAGIKAADRLLTVSHGYAWE	486
357	7489711	400	HF--------------KLYDNI---GG-------DHSNVFAAGLKTADRVVTVSNGYNWE	435
358	8708896	275	-----------YRERFRLYDPI---GG-------EHMNVMKAGLECAHRLVAVSKCYAWE	313
359	15384987	339	HFRL------YDPV-----------GG-------EHSNVFAAGLKMADRAVTVSHGYLWE	374
360	15028467	396	HFRL------YDPV-----------GG-------EHSNVFAAGLKMADPAVTVSHGYLWE	431
361	7489710	434	HFRL------YDPV-----------GG-------EHANIFAAGLKMADRVVTVSRGYLWE	469
362	2833390	489	PFKL------YDPV-----------GG-------EHFNIFAAGLKTADRVVTVSHGYSWE	524
363	7489695	192	-------------EHFRLYDPV---GG-------EHANYFAAGLKMADQVVVVSPGYLWE	228
364	8953573	499	-------------EHFRLYDPV---GG-------EHANYFAAGLKMADQVVVVSPGYLWE	535
365	8953571	500	-------------EHFRLYDFV---GG-------EHANYFAAGLKMADQVVVVSPGYLWE	536
366	7529653	500	-------------EHFRLYDPV---GG-------EHANYFAAGLKMADQVVVTSPGYLWE	536
367	16265834	512	HFKLY-------YDPV---------GG-------EHANIFGAGLKMADRVVVTSPGYLWE	547
368	5825480	500	-------------EHFRLYDPV---GG-------EHANYFAAGLKMADQVVVVSPGYLWE	536
369	2833388	288	SFDF------I----DGYEKPV---KG-------RKINWMKAGILESDRVLTVSPYYAQE	327
370	5441242	286	AFD--------FTDGHLKPV-----RG-------RKINWMKAAILESDLVLTVSPYYAKE	325
371	12003285	284	SFD--------FMDGYEKPV-----KG-------RKINWMKAGIIESDRVLTVSPYYAKE	323
372	267196	287	SFDF------I----DGYEKPV---KG-------RKINWMKAGILESHRVVTVSPYYAQE	326
373	228210	287	SFDF------I----DGYEKPV---KG-------RKINWMKAGILESHRVVTVSFYYAQE	326
374	602594	287	SFDF------I----DGYEKPV---KG-------RKINWMKAGILESHRVVTVSPYYAQE	326
375	16716335	253	KLAFSDGYAKVYTEATPMEEDE---KPPLTGKTYKKINWLKGGIIAADKLVTVSPNYATE	309
376	6136121	288	SFDF-------FDGYEKP---V---KG-------RKINWMKAGILESDRVVTVSPYYAQE	327
377	15223331	290	SFD--------FMDGYEKPV-----KG-------RKINWMKAAILEAHRVLTVSPYYAQE	329
378	15626365	293	SFDFLDGH--VKPIVGRK------------------INWMKAGIIESHRVLTVSPYYAQE	332
379	3832512	287	SFDF------I-------DGYDKPVKG-------RKINWNKAGVLESDRVFTVSPYYAKE	326
380	2833381	288	SFDF------I-------DGYDKPVKG-------RKINWMKAGIREADRVFTVSPNYAYE	327
381	15637079	288	SFDF------I-------DGYDKFVKG-------RKINWMKAGIREALRVFTVSPNYAKE	327
382	2833383	283	SFDF------I----DGYNKPC---EG-------RKINWMKAGILESDQVFTVSPHYAKE	322
383	297424	289	SFDF------I-------DGYDTPVEG-------RKINWMKAGILEADRVLTVSPYYAEE	328
384	82478	289	SFDF------I-------DGYDTPVEG-------RKINWMKAGILEADRVLTVSPYYAEE	328
385	2833385	288	SFDF------I----DGYEKPV---EG-------RKINWMKAGILEADRVLTVSPYYAEE	327
386	2833382	289	SFDF------I-------DGYDTPVEG-------RKINWNKAGILEADRVLTVSPYYAEE	328

[0388]

TABLE XVI
Maize soluble starch synthase I (SSI)
“GLYTR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                  Sequence                  end	#
387	NaizeSSI	376	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPATDKCIP-----CHY-SVDD-	424
388	7489712	376	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPATDKCIP-----CHY-SVDD-	424
389	12019656	383	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPATDKCIP-----CHY-SVDD-	431
390	1549232	377	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPSTDKFLP-----YHY-SVDD-	425
391	2833377	377	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPSTDKFLP-----YHY-SVDD-	425
392	5295947	377	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPSTDKFLP-----YHY-SVDD-	425
393	9369336	383	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLP-----HHY-SVDD-	431
394	6103327	383	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLP-----HHY-SVDD-	431
395	9369334	383	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLP-----HHY-SVDD-	431
396	5880466	383	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLP-----HHY-SVDD-	431
397	7188796	379	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLP-----HHY-SVDD-	427
398	2833387	237	-VTT-A-EG-GQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLP-----HHY-SVDD-	285
399	15237934	387	-ITT-V-EG-GYGLQDLLSSRKSVINGITNGIDINEWNPSTDEHIP-----FHY-SADD-	435
400	6690399	310	-ITT-V-EG-GYGLQDLLSSRKSVINGITNGINVDEWNPSTDEHIP-----FHY-SADD-	358
401	2829792	376	-ITT-P-EG-GYGLHELLSSRQSVLNGITNGIDVNDWNPSTDEHIP-----SHY-SIND-	424
402	15232051	530	-VKT-L-EG-GWGLHNIINENDWKFRGIVNGIDTQEWNPEFDTYLHSDDY-TNY-SLEN-	582
403	3192881	368	-LKT-S-EG-GWGLNGIRNENEWKLQGIVNGIDIEEWNPQLDVYLKSDGY-ANY-SLDT-	420
404	2833384	490	-LKT-S-EG-GWGLHNIINESDWKFRGIVNGVDTKDWNPQFDAYLTSDGY-TNY-NLKT-	542
405	2129898	490	-LKT-S-EG-GWGLHNIINESDWKFRGIVNGVDTKLWNPQFDAYLTSDGY-TNY-NLKT-	542
406	6467503	489	-LKT-S-EG-GWGLHNIINENHWKLQGIVNGIDAKEWNPQFDIQLTSDGY-TNY-SLET-	541
407	14495348	487	-LKT-A-EG-GWGLHGIINESDWKFQGIVNGIDTTDWNPRCDIHLKSDGY-TNY-SLET-	539
408	7489711	436	-LKT-S-EG-GWGLNDIINQNDWKLQGIVNGIDMSEWNPAVD---------VHL-HSDD-	480
409	8708896	314	-CQT-V-EG-GWGLHEVIIKNNWKLRGIVNGIDYKEWNPICDEFLTTDGY-AHY-DVDT-	366
410	15384987	375	-IKT-M-DG-GWGLHEIINIIDWKLQGIVNGIDMAEWNPEVDEHLQSDGY-ANY-TFET-	427
411	15028467	432	-IKT-M-DG-GWGLHEIINENDWKLQGIVNGIDMAEWNPEVDEHLQSDGY-ANY-TFET-	484
412	7489710	470	-LKT-V-EG-GWGLHDIIRSNDWKINGIVNGIDHQEWNPKVDVHLRSDGY-TNY-SLET-	522
413	2833390	525	-LKT-S-QG-GWGLHQIINENDWKLQGIVNGIDTKEWNPELDNHLPRSDGYMNY-SLDT-	578
414	7489695	229	-LKT-V-EG-GWGLHDIIRQNDWKTRGIVNGIDNMEWNPEVDAHLKSDGY-TNF-SLRT-	281
415	8953573	536	-LKT-V-EG-GWGLHDIIRQNDWKTRGIVNGIDNMEWNPEVDAHLKSDGY-TNF-SLGT-	588
416	8953571	537	-LKT-V-EG-GWGLHDIIRQNDWKTRGIVNGIDNNEWNPEVDVHLKSDGY-TNF-SLST-	589
417	7529653	537	-LKT-V-EG-GWGLHDIIRQNDWKTRGIVNGIDNNEWNPEVDVHLKSDGY-TNF-SLRT-	589
418	16265834	548	-LKT-T-EG-GWGLHDIIRENDWKTRGIVGIDYREWNPEVDVHLQSDGYY-ANY-TVAS-	600
419	5825480	537	-LKT-V-EG-GWGLHDIIRQNDWTRGIVNGIDNMEWNPEVDVHLQSDGYY-TNF-SLGT-	589
420	2833388	328	-VIS-GVER-GVELDNFI--RKTGIAGIINGMDVQEWNPVTDKYID-----IHY-DATTV	376
421	5441242	326	-LVS-G-EDRGVELDNII--RKTGVAGIVNGMDIREWSPKTDKYID-----IHF-DTTS-	373
422	12003285	324	LVSG-P-DK-GVELDNIL--RKCTVTGIVNGMDTQEWNPATDKYID-----NHY-DITTV	372
423	267196	327	-LVS-AVDK-GVELDSVL--RKTCITGIVNGMDTQEWNPATDKYTD-----VKY-DITTV	375
424	228210	327	-LVS-AVDK-GVELDSVL--RKTCITGIVNGMDTQEWNPATDKYTD-----VKY-DITTV	375
425	602594	327	-LVS-AVDK-GVELDSVL--RKTCITGIVNGMDTQEWNPATDKYTD-----VKY-DITTV	375
426	16716335	310	-IAADA-AG-GVELDTVI--RAKGIEGIVNGMDIEEWNPKTDKFLS-----VPY-DQNS-	357
427	6136121	328	LVSG-A-EK-GVELDNVIA--KTSITGIVNGMDTQEWNPATDKHID-----TNY-DITTV	376
428	15223331	330	LISG-V-DR-GVELHKYL--RMKTVSGIINGMDVQEWNPSTDKYID-----IKY-DITT-	377
429	15626365	333	LVSG-P-DK-GVELDNIL--RRVGVTGIVNGMDVQEWNPSTDKYIS-----IKY-DASTV	381
430	3832512	327	-LVS-G-EDRGVELDNII--RSIGITGIVNGMDNREWSPQTDRYID-----VHY-DAST-	374
431	2833381	328	LVSC-V-SK-GVELDNHI--RDCGITGICNGMDTQEWNPATDKYLA-----VKY-DITTV	376
432	15637079	328	LVSC-V-SK-GVELDNHI--RDCGITGICNGMDTQEWNPATDKYLA-----VKY-DITTV	376
433	2833383	323	-LIS-G-EDRGVELDNII--RSTGIIGIVNGMDNREWSPQTDRYID-----VHY-NETT-	370
434	297424	329	-LIS-G-IARGCELDNIM--RLTGITGIVNGMDVSEWDPSKDKYIT-----AKYDATTA-	377
435	82478	329	-LIS-G-IARGCELDNIM--RLTGITGIVNGMDVSEWDPSKDKYIT-----AKYDATTA-	377
436	2833385	328	-LIS-G-IARGCELDNIM--RLTGITGIVNGMDVSEWDPSKDKYIA-----VKY-DVSTA	376
437	2833382	329	-LIS-G-IARGCELDNIM--RLTGITGIVNGMDVSEWDPSKDKYIT-----AKYDATTA-	377
387	Maize SSI	425	L---------S-GKAKCKGALQKELGLPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLM-	473
388	7489712	425	L---------S-GKAKCKGALQKELGLPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLM-	473
389	12019656	432	L---------S-GKAKCKSALQKELGLPIRPEVPLIGFIGRLDYQKGIDLIQLIIPHLM-	480
390	1549232	426	L---------S-GKAKCKAELQKELGLPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLM-	474
391	2833377	426	L---------S-GKAKCKAELQKELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDLM-	474
392	5295947	426	L---------S-GKAKCKAELQKELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDLM-	474
393	9369336	432	L---------S-GKAKCKAELQKELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPELM-	480
394	6103327	432	L---------S-GKAKCKAELQKELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPELM-	480
395	9369334	432	L---------S-GKAKCKAELQKELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPELM-	480
396	5880466	432	L---------S-GKAKCKAELQKELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPELM-	480
397	7188796	428	L---------S-GKAKCKAELQRELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPDLM-	476
398	2833387	286	L---------S-GKAKCKAELQKELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPELM-	334
399	15237934	436	V---------S-EKIKCKMALQKELGLPIRFECPMIGFIGRLDYQKGIDLIQTAGPDLM-	484
400	6690399	359	V---------S-EKIKCKMALQKELGLPIRPECPMIGFIGRLDYQKGIDLIQTAGPDLM-	407
401	2829792	425	L---------S-GKVQCKTDLQKELGLPIRPDCPLIGFIGRLDYQKGVDIILSAIFELM-	473
402	15232051	583	L---------HIGKPQCKAALQKYLGLFVRPDVPLIGFIGRLDHQKGVDLIAEAVPWMM-	632
403	3192881	421	LQ--------T-GKPQCKAALQKEMNLPVRDDVPLIGFIGRLDHQKGVDLIAEAIPWMM-	470
404	2833384	543	LQ--------T-GKRQCKAALQRELGLFVREDVPIISFIGRLDHQKGVDLIAEAIPWMM-	592
405	2129898	543	LQ--------T-GKRQCKAALQRELGLPVREDVPIISFIGRLDHQKGVDLIAEAIPWMM-	592
406	6467503	542	LD--------T-GKPQCKTALQNELRFAIPPDVPVIGFIGRLDYQKGVDLIAEAIPWMM-	591
407	14495348	540	VQ--------A-GKQQCKAALQKELGLPVRGDVFVIAFIGRLDHQKGVDLIAEAMPWIA-	589
408	7489711	481	YTNYTFETLDT-GKRQCKAALQRQLGLQVPDDVPLIGFIGRLDHQKGVDIIADAIHWIA-	538
409	8708896	367	L---------AEGKAKCKAALQKELGLPVDPDAPMLGFIGRLDYQKGVDLIRDNYDYIM-	416
410	15384987	428	LD--------T-GKKQCKEALQRQLGLQVRDDVPLIGFIGRLDHQKGVDIIGDAYPWIA-	477
411	15028467	485	LD--------T-GKKQCKEALQRQLGLQVRDDVPLIGFIGRLDHQKGVDIIGDPYPWIA-	534
412	7489710	523	LD--------A-GKRQCKAAJJQRELGLEVRDDVPLLGFIGRLDGQKGVDIIGDYPWIA-	572
413	2833390	579	LQ--------T-GKPQCKAALQKELGLPVRDDVPLIGFIGRLDPQKGVDLIAEAVPWDA-	628
414	7489695	282	LD--------S-GKRQCKEALQRELGLQVRLVPLLGFIGRLDGQKGVEIIAGDAMPWIV-	331
415	8953573	589	LD--------S-GKRQCKEALQRELGLQVRGDVPLLGFIGRLDGQKGVEIIADAMPWIV-	638
416	8953571	590	LD--------S-GKRQCKEALQRELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWIV-	639
417	7529653	590	LD--------S-GKRQCKEALQRELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWIV-	639
418	16265834	601	LD--------S-SKPRCKAALQRELGLEVPDDVPLIGFIGRLDGQKGVDIIGDAMPWIA-	650
419	5825480	590	LD--------S-GKRQCKEALQRELGLQVRNVPLLGFIGRLDGQKGVEIIADDAMPWIV-	639
420	2833388	377	M---------D-AKPLLKEALQAEVGLPVDRNVPLIGFIGRLEEQKGSDIFVAAISQLV-	425
421	5441242	374	VK--------E-AKFLLKEALQAEVGLPVNRDIFLIGFIGRLEEQKGSDILVEAIPKFI-	423
422	12003285	373	M---------D-GKPLLKEALQAEVGLPVDRNVPLVGFIGRLEEQKGSDILVPALHKFI-	421
423	267196	376	M---------D-AKPLLKEALQAAVGLPVDKKIPLIGFIGRLEEQKGSDILVAAIHKFI-	424
424	228210	376	M---------D-AKPLLKEAWQAAVGLPVDKKIPLIGFIGRLEEQKGSDILVAAIHKFI-	424
425	602594	376	M---------D-AKPLLKEALQAAVGLPVDKKVPLIGFIGRLEEQKGSDILVAAINKFI-	424
426	16716335	358	VY--------A-GKAAAKEALQAELGLPVDPTAPLFAFIGRLEEQKGVDIILAALPKIIA	408
427	6136121	377	M---------D-AKPLLKEALQAAVGLPVDKNIPVIGFIGRLEEQKGSDILVAAISKFV-	425
428	15223331	378	V---------TDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKFM-	427
429	15626365	382	L---------E-GKALLKEELQAEVGLPVDKNVPLIAFIGRLEEQKGSDILVEAIPQFI-	430
430	3832512	375	V---------TEAKAILKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILVESIPKFI-	424
431	2833381	377	M---------Q-AKPLLKEALQAAVGLPVDRNIPLIGFIGRLEEQKGSDILYAAISKFI-	425
432	15637079	377	M---------Q-AKPLLKEALQAAVGLPVDRNIPLIGFIGRLEEQKGSDILYAAISKFI-	425
433	2833383	371	V---------TEAKPLLKGTLQAEIGLFVDSSIPLIGFIGRLEEQKGSDILVEAIAKFA-	420
434	297424	378	I---------E-AKALNKEALQAEAGLPVDRKIPLIAFIGRLEEQKGSDVMAAAIPELM-	426
435	82478	378	I---------E-AKALNKEALQAEAGLFVDRKIPLIAFIGRLEEQKGPDVMAAAIPELM-	426
436	2833385	377	V---------E-AKALNKEALQAEVGLFVDRKIPLVAFIGRLEEQKGPDVMMAAIPLLM-	425
437	2833382	378	I---------E-AKALNKEALQAEAGLPVDRKIPLIAFIGRLEEQKGPDVMAAAIPELM-	426
387	Maize SSI	474	REDVQFVMLGSGDPELEDWMRSTESIFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	533
388	7489712	474	REDVQFVMLGSGDPELEDWMRSTESIFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	533
389	12019656	481	RDDVQFVMLGSGDPELEDWMRSTESDFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	540
390	1549232	475	RDNIQFVMLGSGDPGFEGWMRSTESGYRDKFRGWVGFSVPVSNRITAGCDILLMPSRFEP	534
391	2833377	475	RDNIQFVMLGSGDPGFEGWMRSTESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	534
392	5295947	475	RDNIQFVMLGSGDPGFEGWMRSTESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	534
393	9369336	481	REDVQFVMLGSGDPIFEGWMRSTESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	540
394	6103327	481	REDVQFVMLGSGDPIFEGWMRSTESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEF	540
395	9369334	481	REDVQFVMLGSGDPIFEGNMRSTESSYKDKFRGNVGFSVPVSHRITAGCDILLMPSRFEP	540
396	5880466	481	REDVQFVMLGSGDPIFEGWMRSTESSYKDKFRGWVGFSVPVSHRITAGCDILLMFSRFEP	540
397	7188796	477	REDVQFVMLGSGDFVFEGWMRSTESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	536
398	2833387	335	REDVQFVMLGSGDPIFEGWMRSTESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEP	394
399	15237934	485	VDDIQFVMLGSGDPKYESWMRSMEETYRDKFRGWVGFNVPISHRITAGCDILLMPSRFEP	544
400	6690399	408	VDDIQFVMLGSGDFKYESWMRSMEETYRDKFRGWVGFNVFISHRITAGCDILLMPSRFEP	467
401	2829792	474	QNDVQVVMLGSGEKQYEDWMRHTENLFKDKFRAWVGFNVPVSHRITAGCDILLMPSRFEP	533
402	15232051	633	SQDVQLVMLGTGRPDLEEVLRQMEHQYRDKAAGWVGFSVKTAHRITAGADILLMPSRFEP	692
403	3192881	471	GQDVQLVMLGTGRPDLEQMLKQIEGQYGDKVRGWVGFSVKTAHRITAGADILLMPSRFEP	530
404	2833384	593	SHDVQLVMLGTGRADLEQMLKEFEAQHCDKIRSWVGFSVKIAHRITAGSDILLMPSRFEP	652
405	2129898	593	SHDVQLVMLGTGRADLEQMLKEFEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEP	652
406	6467503	592	GQDVQLVMLGTGRQDLEEMLRQFENQHRDKVRGWVGFSVKTAHRITAGADILLMPSRFEP	651
407	14495348	590	GQDVQLIMLGTGRQDLEDTLRRLESQHYDRVRGWVGFSIRLAHRITAGADILLMPSRFEP	649
408	7489711	539	GQDVQLVMLGTGRADLEDMLRRFESSEHSDKVRAWVGFSVPLAHRITAGDILLMPSRFEP	598
409	8708896	417	GEKCQLVMLGSGRQDLEDALPLMENRNKNQCRGWVGFSNKMAHRITAAADILLMPSRFEP	476
410	15384987	478	GQDVQVVMLGTGRPDLEEMLRRFESEHNDKVRGWVGFSVLAHRITAAGADVLLMPSRFEP	537
411	15028467	535	GQDVQVVMLGTGRPDLEEMLRRFESEHNDKVRGWVGFSVQLAERITAGADVLLMPSRFEP	594
412	7489710	573	GQDVQLVMLGTGRADLERMLQHLEREHPNKVRGWVGFSVQLAERITAGADVLVMPSRFEP	632
413	2833390	629	GQDVQLVMLGTGRRDLEQMLRQFECQHNDKIRGWVGFSVKTSHRITAGADVLLMFSRFEP	688
414	7489695	332	SQDVQLVMLGTGRHDLESMLQHFEREHHDKIRGWVGFSVRLAHRITAGADVLLMPSRFEP	391
415	8953573	639	SQDVQLVMLGTGRHDLEGMLRLHFEREHHDKRGWVGFSVRLAHRITAGADMLLMPSRFEP	698
416	8953571	640	SQDVQLVMLGTGRHDLESMLRLFEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEP	699
417	7529653	640	SQDVQLVMLGTGRHDLESMLQHFEREHHDKVRGWVGFSVRLAHRITAGALDALMPSRFEP	699
418	16265834	651	GQDVQLVLLGSGRRDLEVMLQRFEAQHNSKVRGWVGFSVKLAHRITAGADVLVMPSRFEP	710
419	5825480	640	SQDVQLVMLGTGRHDLESMLRHFEREHNDKVRGWVGFSVRLAHRITAGADALLMPSRFEP	699
420	2833388	426	EHNVQIVILGTGKKKFEKQIEHLEVIYPDKARGVAKFNVPLAHMITAGADFMLVPSRFEP	485
421	5441242	424	DQNVQIIILGTGKKSMEKQIEQLEEIYPEKARGIAKFDGPLAHKIIAGSDFIMIPSRFEP	483
422	12003285	422	EMDVQVVILGTGKKEFEKQIEQLEELYPGKAVGVAKFNVPLAHKITAGADFMLVPSRFEP	481
423	267196	425	GLDVQIVVLGTGKKEFEQEIEQLEVLYPNKAKGVAKFNVPLAHMITAGADFMLVPSRFEP	484
424	228210	425	GLDVQIVVLGTGKKEFEQEIEQLEVLYPGKVKGVAKFNVPLAHMITAGADFMLVPSRFEP	484
425	602594	425	GLDVQIVVLGTGKKEFEQEIEQLEVLYPNKAKGVAKFNVPLAHMITAGADFMLVPSRFEP	484
426	16716335	409	TPKVQIAILGTGKAAYEKLVNAIGTKYKGRAKGVVKFSAPLAHMLTAGADFNLVPSRFEP	468
427	6136121	426	GLDVQIIILGTGKKKFEQQIQELEVLYPDKARGVAKFNVPLAHMITAGADFMLVPSRFEP	485
428	15223331	428	GLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSRFEP	487
429	15626365	431	KENVQIVALGTGKKEMEKQLQQLEISYPDKARGVAKFNVPLAHMMIAGADFILIPSRFEP	490
430	3832512	425	DQNVQIIVLGTGKKIMEKQILEQLEVTYPGKAGVAKFNSPLAHKIIAGADFIVIPSRFEP	484
431	2833381	426	SMDVQILILGTGKKKFEQQIEQLEVMYPDKARGVAKFNVPLAHMITAGADFMLIPSRFEP	485
432	15637079	426	SMDVQILILGTGKKKFEQQIEQLEVMYPDKARGVAKFNVPLAHMITAGADFMLIPSRFEP	485
433	2833383	421	DENVQIVVLGTGKKIMEKQIEVLEEKYPGKAIGITKFNSPLAHKIIAGADFIVIPSRFEP	480
434	297424	427	QEDVQIVLLGTGKKKFEKLLKSMEEKYPGKVRAVVKFNAPLAHLIMAGADVLAVPSRFEP	486
435	82478	427	QEDVQIVLLGTGKKKFEKILKSMEEKYPGKVRAVVKFNAPLAHLIMAGADVLAVPSRFEF	486
436	2833385	426	EEDIQIVLLGTGKKKFERALMSAEEKYPDKVPAVVKFNAALAHHIMAGADLLAVTSRFEP	485
437	2833382	427	QEDVQIVLLGTGKKKFEKLLKSMEEKYPGKVPAVVKFNAPLAHLIMAGIDVLAVFSRFEP	486
387	Maize SSI	534	CGLNQLYAMQYGTVPVVHATGGLRDTV-ENFNPF-GENG-EQGTGWAFAFLTTENMFVDI	590
388	7489712	534	CGLNQLYAMQYGTVPVVHATGGLRDTV-ENFNPF-GENG-EQGTGWAFAPLTTENMFVDI	590
389	12019656	541	CGLNQLYAMQYGTVPVVHATGGLRDTV-ENFNPF-GENG-EQGTGWAFAPLTTENMFVDI	597
390	1549232	535	CGLNQLYAMQYGTVPVVHGTGGLRDTV-ENFNPF-AEKG-EQGTGWAFSPLTIEKNAVGI	591
391	2833377	535	CGLNQLYAMQYGTVPVVUGTGGLRDTV-ENFNPF-AEKG-EQGTGWAFSPLTIEKNAVGI	591
392	5295947	535	CGLNQLYANQYGTVPVVHGTGGLRDTV-ENFNPF-AEKG-EQGTGWAFSPLTIEK---GI	588
393	9369336	541	CGLNQLYAMQYGTVPVVHGTGGLRDTV-ETFNPF-GAKG-EEGTGWAFSPLTVEKM	593
394	6103327	541	CGLNQLYAMQYGTVPVVHGTGGLRDTV-ETFNPF-GAKG-EEGTGWAFSPLTVDKM	593
395	9369334	541	CGLNQLYAMQYGTVPVVHGTGGLRDTV-ETFNPF-GAKG-EEGTGWAFSPLTVDKM	593
396	5880466	541	CGLNQLYAMQYGTVPVVHGTGGLRDTV-ETFNPF-GAKG-EEGTGWAFSPLTVDKM	593
397	7188796	537	CGLNQLYAMQYGTVPVVHGTGGLRDTV-ETFNPF-GAKG-EEGTGWAFSPLTVDKM	589
398	2833387	395	CGLNQLYAMQYGTVPVVHGTGGLRDTV-ETFNPF-GAKG-EEGTGWAFSPLTVDKM	447
399	15237934	545	CGLNQLYAMRYGTIPVVHGTGGLPDTV-ENFNPYAEGGA-GTGTGWVFTPLSKDSM	598
400	6690399	468	CGLNQLYAMRYGTIPVVHGTGGLRDTV-ENFNPYAEGGA-GAGTGWVFTPLSKDSM	521
401	2829792	534	CGLNQLYAMRYGTIPIVHSTGGLRDTV-KDFNPY-AQEGIGEGTGWTFSPLTSEKL	587
402	15232051	693	CGLNQLYAMNYGTIPVVHAVGGLRDTV-QQFDPY-SET----GLGWTFDSAEAGKLIHAL	746
403	3192881	531	CGLNQLYAMSYGTVPVVHAVGGLRDTV-QPFDPF-NES----GYGWTFGRAEANQLIDAL	584
404	2833384	653	CGLNQLYANSYGTVPVVHGVGGLRDTV-QPFNPF-DES----GVGWTFDRAEANKLMAAL	706
405	2129898	653	CGLNQLYANSYGTVPVVHGVGGLRDTV-QPFNPF-DES----GVGWTFDRAEANKLMAAL	706
406	6467503	652	CGLNQLYAPMYGTIPVVRAVGGLRDTV-QPFDPF-NES----GLGWTFDSAESHKLIHAL	705
407	14495348	650	CGLNQLYAMMYGTVPVVHAVGGLRDTV-EHYNPY-EES----GLGWTFEKAEARNLIDAL	703
408	7489711	599	CGLNQLYAMAYGTVPVVHAVGGLRDTV-APFDPF-NDT----GLGWTFDRAEANRMIDAL	652
409	8708896	477	CGLNQLYAMAYGTVPIVHSVGGLRDTV-KQYSPF--EN---VGTGWVF	518
410	15384987	538	CGLNQLYAMAYGTVFVVHAVGGLRDTV-APFDPF-ADT----GLGWTFDRAEANRMIDAL	591
411	15028467	595	CGLNQLYAMAYGTVFVVHAVGGLRDTV-APFDPF-ADT----GLGWTFDRAEANRMIDAL	648
412	7489710	633	CGLNQLYAMAYGTVPVVHAVGGLRDTV-APFDPF----G-DAGLGWTFDRAEANKLIEAL	686
413	2833390	689	CALNQLYAMAYGTVPVVHAVGGLRDTV-QPFDP	720
414	7489695	392	CGLNQLYAMAYGTVPVVHAVGGLRDTV-PPFDPF-NHS----GLGWTFDRAEAHKLIEAL	445
415	8953573	699	CGLNQLYAMAYGTVPVVHAVGGLRDTV-PPFDPF-NHS----GLGWTFDRAEAQKLIEAL	752
416	8953571	700	CGLNQLYAMAYGTVPVVHAVGGLRDTV-PPFDPF-NHS----GLGWTFDRAEAHKLIEAL	753
417	7529653	700	CGLNQLYAMAYGTVPVVHAVGGLRDTV-PPFDPF-NHS----GLGWTFDRAEAHKLIEAL	753
418	16265834	711	CGLNQLYAMAYGTVPVVHAVGGLRDTM-SAFDPF-EDT----GLGWTFDRAEFHKLIEAL	764
419	5825480	700	CGLNQLYAMAYGTVPVVHAVGGVRDTV-PPFDPF-NNS----GLGWTFDPAEAHKLIEAL	753
420	2833388	486	CGLIQLHAMRYGTVPIVASTGGLRDTV-K-----------EGYTGFQMGALHVECDKIDS	533
421	5441242	484	CGLVQLHSMPYGTVPIVSSTGGLVDTVQEGFTGF	517
422	12003285	482	CGLIQLHANRYGTIPICASTGGLVDTVKEGFTGF	515
423	267196	485	CGLIQLHAMRYGTVPICASTGGLVDTV-K	512
424	228210	485	CGLIQLHAMRYGTVPICASTGGLVDTV-K	512
425	602594	485	CGLIQLHAMRYGTVPICASTGGLVDTV-K	512
426	16716335	469	CGLIQLHANHYGTVPVVASTGGLVDTV-K	496
427	6136121	486	CGLIQLHAMRYGTIPICASTGGLVDTVTEGFTGF	519
428	15223331	488	CGLIQLHANRYGTVPIVASTGGLVDTV-KD	516
429	15626365	491	CGLIQLQAIVIRYGTVPIVASTGGLVDTVKEGFTGF	524
430	3832512	485	CGLVQLHANFYGTVPIVSSTGGLVDTV-K-----------EGYTGFHVGAFSVECEAVDP	532
431	2833381	486	CGLIQLHAMRYGTPCICASTGGLVDTV-K	513
432	15637079	486	CGLIQLHAMRYGTPCICASTGGLVDTV-K	513
433	2833383	481	CGLVQLHAMPYGTVPIVSSTGGLVDTV-K-----------EGYTGFHAGPFDVECEDVD	527
434	297424	487	CGLIQLQGMRYGTPCACASTGGLVDTV	513
435	82478	487	CGLIQLQGMRYGTACACASTGGLVDTV	513
436	2833385	486	CGLIQLQGMRYGTPCACASTGGLVDTI	512
437	2833382	487	CGLIQLQGMRYGTPCACASTGGLVDTV	513

[0389]

TABLE XVII
Maize soluble starch synthase I (SSI)
“CTEND” Domain Alignments
with other similar proteins
SEQ	Acces-			a.
Id.	sion	a.a		a.
No.	Number	#	(start)       Sequence       end	#
438	MaizeSSI	591	ANCNIYIQGTQVLLGRANEARHVKRLHVGPCR	622
439	7489712	591	ANCNIYIQGTQVLLGRANEARHVKRLHVGPCR	622
440	12019656	598	ANCNFDIQGAQIFLGRANEEGHVKRLIWGPCR	629
441	1549232	592	ADGNFDIQGTQVLLGGSNEARHVKRLYMGPCR	623
442	2833377	592	ADGNFDIQGTQVLLGGSNEARHVKRLYMGPCR	623
443	5295947	589	ADGNFDIQGTQVLLGGSNEARHVKRLYMGPCR	620
444	15232051	747	GNC	749
445	3192881	585	GNC	587
446	2833384	707	WNC	709
447	2129898	707	WNC	709
448	6467503	706	GHC	708
449	14495348	704	GHC	706
450	7489711	653	SHC	655
451	15384987	592	GHC	594
452	15028467	649	GHC	651
453	7489710	687	RHC	689
454	7489695	446	GHC	448
455	8953573	753	GHC	755
456	8953571	754	GHC	756
457	7529653	754	GHC	756
458	16265834	765	GHC	767
459	5825480	754	GHC	756
460	2833388	534	ADVAAIVKTVARALG	548
461	3832512	533	AD	534

[0390]

TABLE XVIII
Identities of the Accession Numbers used in Tables. XIV-XVII.
Accession	Brief Description of sequences
Id.	producing significant alignments	score	E-value
gi|7489712|pir||T01414	ADPglucose - starch glucosyltransfera . . .	1189	0.0
gi|12019656|gb|AAD45815.2|	(AF168786) soluble starch syntha . . .	1145	0.0
gi|1549232|dbj|BAA07396.1|	(D38221) SSS1 [Oryza sativa] >gi . . .	998	0.0
gi|2833377|sp|Q40739|UGS2_ORYSA	Soluble glycogen [starch] s . . .	996	0.0
gi|5295947|dbj|BAA81848.1|	(AB026295) ESTs AU075322 (C11109) . . .	992	0.0
gi|9369336|emb|CAB99210.1|	(AJ292522) starch synthase I-2 [. . .	953	0.0
gi|6103327|gb|AAF03557.1|	(AF091802) starch synthase I [Aeg . . .	953	0.0
gi|9369334|emb|CAB99209.1|	(AJ292521) starch synthase I-1 [. . .	952	0.0
gi|5880466|gb|AAD54661.1|	(AF091803) starch synthase I [Tri . . .	951	0.0
gi|7188796|gb|AAF37876.1|AF234163_1	(AF234163) starch synth . . .	949	0.0
gi|2833387|sp|Q43654|UGS2_WHEAT	Soluble glycogen [starch] s . . .	884	0.0
gi|15237934|ref|NP 197818.1|	(NM_122336) soluble starch syn . . .	744	0.0
gi|6690399|gb|AAF24126.1|AF121673_1	(AF121673) soluble star . . .	741	0.0
gi|2829792|sp|P93568|UGS2_SOLTU	Soluble glycogen [starch] s . . .	724	0.0
gi|15232051|ref|NP_186767.1|	(NM_110984) putative glycogen . . .	447	e−124
gi|3192881|gb|AAC19119.1|	(AF068834) starch synthase [Ipomo . . .	443	e−123
gi|2833384|sp|Q43093|UGS3_PEA	Glycogen [starch] synthase, c . . .	431	e−120
gi|2129898|pir||S61505	UDPglucose - starch glucosyltransfera . . .	431	e−119
gi|6467503|gb|AAF13168.1|AF173900_1	(AF173900) granule boun . . .	430	e−119
gi|14495348|gb|AAK64284.1|AF383878_1	(AF383878) soluble sta . . .	426	e−118
gi|7489711|pir||T01209	ADPglucose - starch glucosyltransfera . . .	421	e−117
gi|8708896|gb|AAC17970.2|	(AF026421) soluble starch synthas . . .	419	e−116
gi|15384987|emb|CAC59826.1|	(AJ308110) soluble starch synth . . .	419	e−116
gi|15028467|gb|AAK81729.1|AF395537_1	(AF395537) soluble sta . . .	416	e−115
gi|7489710|pir||T01208	ADPglucose - starch glucosyltransfera . . .	414	e−114
gi|2833390|sp|Q43847|UGS3_SOLTU	Glycogen [starch] synthase, . . .	410	e−113
gi|7489695|pir||T06798	probable starch synthase (EC 2.4.1.— . . .	405	e−112
gi|8953573|emb|CAB96627.1|	(AJ269504) starch synthase IIa-3 . . .	388	e−107
gi|8953571|emb|CAB96626.1|	(AJ269503) starch synthase IIa-2 . . .	388	e−107
gi|7529653|emb|CAB86618.1|	(AJ269502) starch synthase IIa-1 . . .	388	e−107
gi|16265834|gb|AAL16661.1|AF419099_1	(AF419099) putative so . . .	387	e−106
gi|5825480|gb|AAD53263.1|AF155217_1	(AF155217) starch synth . . .	387	e−106
gi|2833388|sp|Q43784|UGST_MANES	Granule-bound glycogen [sta . . .	340	3e−92
gi|5441242|dbj|BAA82346.1|	(AB029546) granule-bound starch . . .	334	2e−90
gi|12003285|gb|AAG43519.1|	AF210699_1 (AF210699) granule-bou . . .	325	7e−88
gi|267196|sp|Q00775|UGST_SOLTU	Granule-bound glycogen [star . . .	325	8e−88
gi|228210|prf||1718316A	granule-bound starch synthase [Sola . . .	324	2e−87
gi|602594|emb|CAA58220.1|	(X83220) starch (bacterial glycog . . .	324	2e−87
gi|16716335|gb|AAC17969.3|	(AF026420) granule-bound starch . . .	322	7e−87
gi|6136121|sp|O82627|UGST_ANTMA	Granule-bound glycogen [sta . . .	321	2e−86
gi|15223331|ref|NP_174566.1|	(NM_103023) starch synthase, p . . .	317	3e−85
gi|15626365|emb|CAC69955.1|	(AJ345045) granule-bound starch . . .	315	1e−84
gi|3832512|gb|AAC70779.1|	(AF097922) granule-bound glycogen . . .	313	4e−84
gi|2833381|sp|Q42857|UGST_IPOBA	Granule-bound glycogen [sta . . .	311	9e−84
gi|15637079|dbj|BAB68126.1|	(AB071604) granule-bound starch . . .	311	1e−83
gi|2833383|sp|Q43092|UGST_PEA	Granule-bound glycogen [starc . . .	310	4e−83
gi|297424|emb|CAA46294.1|	(X65183) glycogen (starch) syntha . . .	303	4e−81
gi|82478|pir||JQ0703	UDPglucose - starch glucosyltransferase . . .	303	5e−81
gi|2833385|sp|Q43134|UGST_SORBI	Granule-bound glycogen [sta . . .	303	5e−81
gi|2833382|sp|Q42968|UGST_ORYGL	Granule-bound glycogen [sta . . .	302	6e−81
gi|136758|sp|P19395|UGST_ORYSA	Granule-bound glycogen [star . . .	302	6e−81
gi|15900991|ref|NP_345595.1|	(NC_003028) glycogen synthase . . .	302	6e−81
gi|7798551|gb|AAC61675.2|	(AF031162) granule-bound starch s . . .	301	1e−80
gi|15903076|ref|NP_358626.1|	(NC_003098) Glycogen synthase . . .	301	1e−80
gi|136757|sp|P04713|UGST_MAIZE	Granule-bound glycogen [star . . .	300	2e−80
gi|4760582|dbj|BAA77351.1|	(AB019623) starch synthase (GBSS . . .	300	4e−80
gi|11037536|gb|AAG27624.1|AF286320_1	(AF286320) granule bou . . .	300	4e−80
gi|6624287|dbj|BAA88512.1|	(AB029064) starch synthase (GBSS . . .	296	3e−79
gi|6492245|gb|AAF14233.1|AF109395_1	(AF109395) granule-boun . . .	296	4e−79
gi|6624281|dbj|BAA88509.1|	(AB029061) starch synthase (GBSS . . .	296	4e−79
gi|297422|emb|CAA45472.1|	(X64108) starch granule-bound sta . . .	295	9e−79
gi|6624283|dbj|BAA88510.1|	(AB029062) starch synthase (GBSS . . .	294	1e−78
gi|6624285|dbj|BAA88511.1|	(AB029063) starch synthase (GBSS . . .	294	2e−78
gi|4588609|gb|AAD26156.1|AF113844_1	(AF113844) granule-boun . . .	294	2e−78
gi|18652407|gb|AAL77109.1|AF474373_6	(AF474373) granule-bou . . .	294	2e−78
gi|136755|sp|P09842|UGST_HORVU	Granule-bound glycogen [star . . .	293	3e−78
gi|4760584|dbj|BAA77352.1|	(AB019624) starch synthase (GBSS . . .	293	4e−78
gi|6318540|gb|AAF06937.1|AF110374_1	(AF110374) granule-boun . . .	293	5e−78
gi|17736918|gb|AAL41028.1|	(AF250137) mutant granule bound . . .	292	7e−78
gi|6318538|gb|AAF06936.1|AF110373_1	(AF110373) granule-boun . . .	290	4e−77
gi|4760580|dbj|BAA77350.1|	(AB019622) starch synthase (GBSS . . .	290	4e−77
gi|136765|sp|P27736|UGST_WHEAT	Granule-bound glycogen [star . . .	288	2e−76
gi|15672681|ref|NP_266855.1|	(NC_002662) glycogen synthase . . .	287	3e−76
gi|17366711|sp|Q9CHM9|GLGA_LACLA	Glycogen synthase (Starch . . .	286	4e−76
gi|18139611|gb|AAL58572.1|	(AY069940) granule binding starc . . .	285	1e−75
gi|16080147|ref|NP_390973.1|	(NC_000964) starch (bacterial . . .	281	1e−74
gi|4588607|gb|AAD26155.1|AF113843_1	(AF113843) granule-boun . . .	278	1e−73
gi|7484400|pir| |T07924	probable starch synthase (EC 2.4.1.- . . .	277	2e−73
gi|2811062|sp|O08328|GLGA_BACST	Glycogen synthase (Starch [. . .	270	3e−71
gi|18309046|ref|NP_560980.1|	(NC_003366) glycogen synthase . . .	263	5e−69
gi|15895507|ref|NP_348856.1|	(NC_003030) Glycogen synthase, . . .	256	6e−67
gi|15966599|ref|NP_386952.1|	(NC_003047) PROBABLE GLYCOGEN . . .	248	1e−64
gi|15643657|ref|NP_228703.1|	(NC_000853) glycogen synthase . . .	247	3e−64
gi|16766821|ref|NP_462436.1|	(NC_003197) glycogen synthase . . .	242	1e−62
gi|15613648|ref|NP_241951.1|	(NC_002570) starch (bacterial . . .	241	2e−62
gi|16762766|ref|NP_458383.1|	(NC_003198) glycogen synthase . . .	240	3e−62
gi|15641730|ref|NP_231362.1|	(NC_002505) glycogen synthase . . .	239	5e−62
gi|15803938|ref|NP_289974.1|	(NC_002655) glycogen synthase . . .	237	3e−61
gi|16124067|ref|NP_407380.1|	(NC_003143) glycogen synthase . . .	235	1e−60
gi|15890897|ref|NP_356569.1|	(NC_003063) AGR_L_1562p [Agrob . . .	228	1e−58
gi|16119514|ref|NP_396220.1|	(NC_003064) AGR_pAT_410p [Agro . . .	226	8e−58
gi|17938870|ref|NP_535658.1|	(NC_003306) glycogen synthase . . .	225	9e−58
gi|9587341|gb|AAF89272.1|AF285997_1	(AF285997) granule-boun . . .	222	1e−56
gi|9587301|gb|AAF89252.1|AF285977_1	(AF285977) granule-boun . . .	221	1e−56
gi|9587321|gb|AAF89262.1|AF285987_1	(AF285987) granule-boun . . .	221	2e−56
gi|9587293|gb|AAF89248.1|AF285973_1	(AF285973) granule-boun . . .	221	2e−56
gi|15606118|ref|NP_213495.1|	(NC_000918) glycogen synthase . . .	220	3e−56
gi|9587343|gb|AAF89273.1|AF285998_1	(AF285998) granule-boun . . .	220	3e−56
gi|15602409|ref|NP_245481.1|	(NC_002663) GlgA [Pasteurella . . .	219	7e−56
gi|9587317|gb|AAF89260.1|AF285985_1	(AF285985) granule-boun . . .	218	1e−55

[0391]

Maize Soluble Starch Synthase IIa (SSIIa)
Accession: AF019296
NID:       g2811133
Mol. wt. (calc) = 80156    Residues = 732
1	M S S A A V S S S S S T F F L A L A S A S P G G R R R A R V	SEQ. ID. No. 462
31	G S S P F H T G A S L S F A F W A P P S P P R A P R D A A L
61	V R A E A E A G G K D A P P E R S G D A A R L P R A R R N A
91	V S K R R D P L Q P V G R Y G S A T G N T A R T G A A S C Q
121	N A A L A D V E I K S I V A A P P T S I V K F P A P G Y R M
151	I L P S G D I A P E T V L P A P K P L H E S P A V D G D S N
181	G I A P P T V E P L V Q E A T W D F K K Y I G F D E P D E A
211	K D D S R V G A D D A G S F E H Y G D N D S G P L A G E N V
241	M N V I V V A A E C S P W C K T G G L G D V V G A L P K A L
271	A R R G H R V M V V V P R Y G D Y V E A F D M G I R K Y Y K
301	A A G Q D L E V N Y F H A F I D G V D F V F I D A P L F R H
331	R Q D D I Y G G S R Q E I M K R M T L F C K V A V E V P W H
361	V P C G G V C Y G D G N L V F I A N D W H T A L L P V Y L K
391	A Y Y R D H G L M Q Y T R S V L V I H N I A H Q Q R G P V D
421	E F P Y M D L P E H Y L Q H F E L Y D P V G G E H A N I F A
451	A G L K M A D R V V T V S R G Y L W E L K T V E G G W G L H
481	D I I R S N D W K I N G I V N G I D H Q E W N P K V D V H L
511	R S D G Y T N Y S L E T L D A G K R Q C K A A L Q R E L G L
541	E V R D D V P L L G F I G R L D G Q K G V D I I G D A M P W
571	I A G Q D V Q L V M L G T G R A D L E R M L Q H L E R E H P
601	N K V R G W V G F S V P M A H R I T A G A D V L V M P S R F
631	E P C G L N Q L Y A M A Y G T V P V V H A V G G L R D T V A
661	P F D P F G D A G L G W T F D R A E A N K L I E A L R H C L
691	D T Y R K Y G E S W K S L Q A R G M S Q D L S W D H A A E L
721	Y E D V L V K A K Y Q W

[0392]

TABLE XIX
Maize soluble starch synthase IIa (SSIIa)
Alignments with other similar proteins—Transit Peptide
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                Sequence                ending	#
463	MAIZE SSIIa	34	PFHTGXXXXXXXXXXXXXXXXXXXXXXXVRAEAEAGGKDAPPER--SGDAARLPRARRBAV	91
464	7489710	34	PFHTGASLSFAFWAPPSPPPRAPRDAALVRAEAEAGGKDAPPER--SGDAARLPRARRNAV	91
465	8953573	101	DAAEGGAPSPPAP--RQEDARLPSMNGMPV	128
466	8953571	102	DAAEGGAPAPPAP--RQDAARPPSMNGTPV	129
467	5825480	102	DAAEGGAPAPPAP--RQDAARPPSMNGTPV	129
468	7529653	102	DAAEGGGPSPPAA--RQDAARPPSMNGMPV	129
469	16265834	78	AAVERAGEDDDEEEEFSSGAWQPPRSRRGGV	108
470	7489711	38	--SGAELRLHWARRGPP	52
471	7188796	21	PAARATAC	28
472	2833377	16	APQVR--PGRRLRLQRVRRRCV	35
473	1549232	16	APQVR--PGRRLRLQRVRRRCV	35
474	5295947	16	APQVR--PGRRLRLQRVRRRCV	35

[0393]

TABLE XX
Maize soluble starch synthase ha (SSIIa)
“GLASS” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)	Sequence	end	#
475	MaizeSSIIa	92	S---K-RRDPLQPVG-RYGSATGNTAR-------------------TG---A--------	116
476	7489710	92	S---K-RRDPLQPVG-RYGSATGNTAR-------------------TG---A--------	116
477	8953573	129	N---G-ENKSTGGGGATKDSGLPAPAR-------------------AP---Q--------	154
478	8953571	130	N---G-ENKSTGGGGATKDSGLPAPAR-------------------AP---H--------	155
479	5825480	130	N---G-ENKSTGGGGATKDSGLPAPAR-------------------AP---H--------	155
480	7529653	130	N---G-ENKSTGGGGATKDSGLPTPAR-------------------AP---H--------	155
481	16265834	109	GKVLK-RRGTVPPVG-RYGSG-GDAARVRGAAAPAPAPTQDAASSKNG---ALLSGRDDD	162
482	15028467	67	G-EDGVAKAKTKS-------------------AGSSKA--------	84
483	7489711	53	Q---D-GAASVRAAA-APAGGESEEAA-------------------KS---S--------	77
484	5880466	42	G-RY---VAELSR-------------------EG---P--------	53
485	9369334	42	G-RY---VAELSR-------------------EG---P--------	53
486	6103327	42	G-RY---VAELSR-------------------EG---P--------	53
487	7188796	29	V---V-RARLRRVAR-GRYVA--ELSR-------------------EG---P--------	51
488	2833377	36	A---ELSRDG--------GSAHGPLA----------------------------------	50
489	1549232	36	A---E-------------------LSR-------------------DG---G--------	43
490	5295947	36	A---E-------------------LSR-------------------DG---G--------	43
475	MaizeSSIIa	117	CQNAALADVEIKSI------VAAP--PTSIVKFPAPGY--RMIL---PSGD--I---A--	158
476	7489710	117	-AS--CQNAA ADVEIKSI-VAAP--PTSIVKFPAPGY--RMIL---PSGD--I---A--	158
477	8953573	155	-PS--SQNRVPVNGENKAN-VASP--PTSIAEVAAPDP--AATI---SISD--K---A--	196
478	8953571	156	-PS--TQNRVPVNGENKAN-VASP--PTSIAEVVAPDS--AATI---SISD--K---A--	197
479	5825480	156	-PS--TQNRVPVNGENKAN-VASP--PTSIAEVVAPDS--AATI---SISD--K---A--	197
480	7529653	156	-PS--TQNRAPVNGENKAN-VASP--PTSIAEAAASDS--AATI---SISD--K---A--	197
481	16265834	163	TPA--SRNGSVVTGADKPA-AATP--PVTITKLPAPDS--PVIL---PSVD--K---PQP	207
491	15384987	65	---A--	65
482	15028467	85	-VA--VQGSTAKADHVEDS-VSSP-------KYVKPA----VAK---QNGE--VVSRA--	122
483	7489711	78	-SS--SQAGAVQGSTAKAVDSASP--PNPLTSAPKQSQ--SAANQNGTSGG--S---S--	123
492	15232051	181	ASVISSSP-VTSPQKPSDVATNGKPWS--SVVA---SSVDPPY---K--	218
484	5880466	54	-AARPAQQQQL-----------AP--PL------VPGFLAPPPP---APAQ--S---P--	83
485	9369334	54	-AARPAQQQQL-----------AP--PL------VPGFLAPPPP---APAQ--S---P--	83
486	6103327	54	-AARPAQQQQL-----------AP--PL------VPGFLAPPPP---APAQ--S---P--	83
487	7188796	52	-AA--RPAQQL-----------AP--PV------VPGFLAPPPP---APAQ--S---P--	79
488	2833377	51	--------------------------PAPLVKQP--------VL---PTF---L---V--	65
489	1549232	44	-SA--QRPLA----------------PAPLVKQP--------VL---PTF---L---V--	65
490	5295947	44	-SA--QRPLA----------------PAPLVKQP--------VL---PTF---L---V--	65
475	MaizeSSIIa	159	------------------PETVLPAPK----------PLHE---S----------PAVDG	177
476	7489710	159	------------------PETVLPAPK----------PLHE---S----------PAVDG	177
477	8953573	197	------------------PESVVPAEKAPPSSGSNFVPSAS---A----------PGSDT	225
478	8953571	198	------------------PESVVPAEK----------PPPS---SGSNFVVSASAPRLDI	226
479	5825480	198	------------------PESVVPAEK----------PPPS---SGSNFVVSASAPRLDI	226
480	7329653	198	------------------PESVVPA--------------EE---T----------PPSSG	212
481	16265834	208	EFVIPDATAPAPPPPGSNPRSSAPLPK----------PDNS---E----------FAEDK	244
491	15384987	66	------------------TKSDAPVFK----------PKVD---PSV--------PASKA	86
482	15028467	123	------------------TKSDAPVSK----------PKVD---PSV--------PASKA	143
483	7489711	124	------------------ASTAAPVSG----------PKAD---H----------PSAPV	142
492	15232051	219	------------------PSSVMTSPE----------KTSDPVTS----------PGKPS	240
484	5880466	84	------------------APTQPPLPD----------AGVG---E----------LAPDL	102
485	9369334	84	------------------APTQPPLPD----------AGVG---E----------LAPDL	102
486	6103327	84	------------------APTQPPLPD----------AGVG---E----------LAPDL	102
487	7188796	80	------------------APTQPPLPD----------AGVG---E----------LAPDL	98
493	7489712	49	PAPE----------PLPP---A----------LLAPP	62
488	2833377	66	------------------PTSTPPAPTQSP-------APAP---T----------PPPLP	87
489	1549232	66	------------------PTSTPPAPTQSP-------APAP---T----------PPPLP	87
490	5295947	66	------------------PTSTPPAPTQSP-------APAP---T----------PPPLP	87
475	Maize SSIIa	178	---DSN-------------------GI--A------P-----------------------	184
476	7489710	178	---DSN-------------------GI--A------P-----------------------	184
477	8953573	226	---VSDVELELKKGAVIVKEAPNPKAL--S------P-----------------------	251
478	8953571	227	---DSDVEPELKKGAVIVEEAPNPKAL--S------P-----------------------	252
479	5825480	227	---DSDVEPELKKGAVIVEEAPNPKAL--S------P-----------------------	252
480	7529653	213	SNFESS-------------------AS--A------PGSDTVSDVEQELKKGAVVVEEAP	245
481	16265834	245	---SAK-------------------VVESA------PKPKATR-----------------	259
491	15384987	87	---EAD-------------------GN--A------------------------------	92
482	15028467	144	---EAD-------------------GN--A------------------------------	149
483	7489711	143	---TKR-------------------EI--D------A-----------------------	149
492	15232051	241	---KSR-------------------AG--AFWSDPLP-----------------------	253
484	5880466	103	---LLE-------------------GI--A------E-----------------------	109
485	9369334	103	---LLE-------------------GI--A------E-----------------------	109
486	6103327	103	---LLE-------------------GI--A------E-----------------------	109
487	7188796	99	---LLE-------------------GI--A------E-----------------------	105
493	7489712	63	---LVP-------------------GF--L------A-----------------------	69
488	2833377	88	---DSGV------------------GE--I------E-----------------------	95
489	1549232	88	---DSGV------------------GE--I------E-----------------------	95
490	5295947	88	---DSGV------------------GE--I------E-----------------------	95
475	MaizeSSIIa	185	------PTVEPLVQEATWDFK--KYIGFDEP---DEAKDDSRVGADDAGSFEH-YGDN--	230
476	7489710	185	------PTVEPLVQEATWDFK--KYIGFDEP---DEAKDDSRVGADDAGSFEH-YGDN--	230
477	8953573	252	------PAA-PAVQQDLWDFK--KYIGFEEP---VEACDDGPAVALDAGSFEH-HQNH--	296
478	8953571	253	------PAA-PAVQEDLWDFK--KYIGFEEP---VEAKDDGWAVADDAGSFEH-HQNH--	297
479	5825480	253	------PAA-PAVQEDLWDFK--KYIGFEEP---VEAKDDGWAVADDAGSFEH-HQNH--	297
480	7529653	246	KPKALSPPAAPAVQEDLWDFK--KYIGFEEP---VEAKDDGRAVADDAGSFEH-HQNH--	297
481	16265834	260	------SSPIPAVEEETWDFK--KYFDLNEPDAAEDGDDDDDWADSDASDSEI-DQDD--	308
491	15384987	93	---------QAVESKAALDKK--EDVGVAEP---LEAKADAGGDAGAVSSADD-SENK--	135
482	15028467	150	---------QAVESKAALDKK--EDVGVAEP---LEAKADAGGDAGAVSSADD-SENK--	192
483	7489711	150	------SAVKP--EPAGDDARPVESIGIAEP---VDAKADAAPATDAAASAPYDREDN--	196
494	2833384	217	IRTSSLKFE--NFEGANEP--------SSKEVANEAENFES-GGEK--	251
495	2129898	217	IRTSSLKFE--NFEGANEP--------SSKEVANEAENFES-GGEK--	251
496	15232051	254	------SYLTKAPQTSTMKTE--KYVE-KTP---DVASSETNEPGKD---------EE--	290
497	3192881	107	DHR---ESSSKEIDVGTEDPVN-----EDL--	128
498	2833390	284	DE--	285
499	14495348	247	D--	247
484	5880466	110	------DSIDSIIVAASEQDS--EIMDANEQ---PQAK----------------------	136
485	9369334	110	------DSIDSIIVAASEQDS--EIMDANEQ---PQAK----------------------	136
486	6103327	110	------DSIDSIIVAASEQDS--EIMDANEQ---PQAK----------------------	136
487	7188796	106	------DSIDTIVVAAS--------------------EQDSEI------------MDA--	125
493	7489712	70	------PPAEPTGEPAS--------TPPPVP---DAGLGD--LGLEPEGIAEG-SIDNTV	109
488	2833377	96	------PDLEGLTEDSI-DKT--IFVASEQE---SEIMDVKEQA----------------	127
489	1549232	96	------PDLEGLTEDSI-DKT--IFVASEQE---SEIMDVKEQA----------------	127
490	5295947	96	------PDLEGLTEDSI-DKT--IFVASEQE---SEIMDVKEQA----------------	127
500	6136121	54	RNNAKQSRSLVK-KTDN--	69
501	15637079	65	EN--	66
475	Maize SSIIa	231	------DS--GPLAGEN------V-----MNVIVVAAECSPWCKTGGLGDVVGALPKALA	271
476	7489710	231	------DS--GPLAGEN------V-----MNVIVVAAECSPWCKTGGLGDVVGALPKALA	271
477	8953573	297	------DS--GPLAGEN------V-----MNVVVVAAECSPWCKTGGLGDVAGALPKALA	337
478	8953571	298	------DS--GPLAGEN------V-----MNVVVVAAECSPWCKTGGLGDVAGALPKALA	338
479	5825480	298	------DS--GPLAGEN------V-----MNVVVVAAECSPWCKTGGLGDVAGALPKALA	338
480	7529653	298	------DS--GPLAGEN------V-----MNVVVVAAECSPWCKTGGLGDVAGALPKALA	338
481	16265834	309	------DS--GPLAGEN------V-----MNVIVVAAECSPWCKTGGLGDVAGALPKALA	349
502	7489695	1	NVVVVAAECSPWCKTGGLGDVAGAIPKALA	30
491	15384987	136	------ES--GPLAGPN------V-----MNVIVVASECSPFCKTCGLGDVVGALPKALA	176
482	15028467	193	------ES--GPLAGPN------V-----MNVIVVASECSPFCKTGGLGDVVGALPKALA	233
483	7489711	197	------EP--GPLAGPN------V-----MNVVVVASECAPFCKTGGLGDVVGALPKALA	237
494	2833384	252	------PP---PLAGTN------V-----MNIILVSAECAPWSKTGGLGDVAGSLPKALA	291
495	2129898	252	------PP---PLAGTN------V-----MNIILVSAECAPWSKTGGLGDVAGSLPKALA	291
503	6467503	253	PLAGDN------V-----MNVILVAAECAPWSKTGGLGDVAGSLPKALA	290
496	15232051	291	------KP--PPLAGAN------V-----MNVILVAAECAPFSKTGGLGDVAGALPKSLA	331
497	3192881	129	------KP--PPLAGTN------V-----MNVILVCAECAPWSKTGGLGDVAGALPKALA	169
498	2833390	286	------KP--PPLAGTN------V-----MNIILVASECAPWSKTGGLGDVAGALPKALA	326
499	14495348	248	------DP--SASASVD------L-----INIILVAAECAPWSKTGGLGDVAGALPKALA	288
504	8708896	78	PLAGPN------V-----MNVVMVGAECAPWSKTGGLGDVMAALPKALV	115
525	2833387	1	EAAPYAKSGGLGDVCGSLPKALA	23
492	15237934	139	EV------V-----NNLVFVTSEAAPYSKTGGLGDVCGSLPIALA	172
484	5880466	137	V------T-----RSIVFVTGEAAPYAKSGGLGDVCGSLPIALA	169
505	6690399	62	EV------V-----NNLVFVTSEAAPYSKTGGLGDVCGSLPIALA	95
485	9369334	137	----------------V------T-----RSIVFVTGEAAPYAKSGGLGDVCGSLPIALA	169
486	6103327	137	----------------V------T-----RSIVFVTGEAAPYAKSGGLGDVCGSLPIALA	169
487	7188796	126	------ND--QPLA-KV------T-----RSIVFVTGEAAPYAKSGGLGDVCGSLPIALA	165
506	2829792	132	NIIFVTAEAAPYSKTGGLGDVCGSLPMALA	161
507	12019656	140	SIVFVTGEASPYAKSGGLGDVCGSLPVALA	169
493	7489712	110	VVASEQDS--EIVVGKEQARAKVT-----QSIVFVTGEASPYAKSGGLGDVCGSLPVALA	162
488	2833377	128	-------------QAKV------T-----RSVVEVTGEASPYAKSGGLGDVCGSLPIALA	163
489	1549232	128	-------------QAKV------T-----RSVVFVTGEASPYAKSGGLGDVCGSLPIALA	163
490	5295947	128	-------------QAKV------T-----RSVVFVTGEASPYAKSGGLGDVCGSLPIALA	163
500	6136121	70	------GSPLGKIICGT------G-----MNLVFVLAEVGPWSKTGGLGDVVGGLPPAMA	112
508	5441242	80	-----MNLIFVGAEVAPWSKTGGLGDVLGGLPSALA	110
509	2833388	82	-----MNLIFVGAEVGPWSKTGGLGDVLGGLPPALA	112
510	3832512	79	N------G-----MNLVFVGAEVGPWSKTGGLGDVLGGLPPALA	111
511	15223331	75	A--GKIVCEK------G-----MSVIFIGAEVGPWSKTGGLGDVLGGLPPALA	114
512	2833383	77	-----MSLVEVGAEVCPWSKTGGLGDVLGGLPPVLA	107
513	15637079	67	------EG--GMAAGTI------VCKQQGMNLVFVGCEVGPWCKTGGLGDVLGGLPPALA	112
514	267196	81	-----MNLIFVGTEVGPWSKTGGLGDVLGGLPPALA	111
515	228210	81	-----MNLIFVGTEVGPWSKTGGLGDVLGGLPPALA	111
516	602594	81	-----MNLIFVGTEVGPWSKTGGLGDVLGGLPPALA	111
517	283338	182	-----MNLVFVGCEEGPWCKTGGLGDVLGGLPPALA	112
518	1200328	578	-----MTLIFVSAECGPWSKTGGLGDVVGGLPPALA	108
519	1562636	587	-----MNLIFVGTEVAPWSKTGGLGDVLGGLPPALS	117
520	662428	173	GSG------G-----NNLVFVGAEMAPWSKTGGLGDVLGGLPAAMA	107
521	662428	773	GSG------G-----MNLVFVGAEMAPWSKTGGLGDVLGGLPPAMA	107
522	476058	473	GEG------G-----MNLVFVGAEMAPWSKTGGLGDVLGGLPPAMA	107
523	1813961	174	PIVCSA------G-----MTIIFIATECHPWCKTGGLGDVLGGLPPALA	111
524	17736918	3	GSG------G-----MNLVFVGAEMAPWSKTGGLGDVLGGLPPAMA	37
475	Maize SSIIa	272	RRGHRVMVVVPRY-------GD-------YVEAFDMGIRKYYKAAGQDLEVNYFHAFIDG	317
476	7489710	272	RRGHRVMVVVPRY-------GD-------YVEAFDMGIRKYYKAAGQDLEVNYFHAFIDG	317
477	8953573	338	KRGHRVMVVVPRY-------GD-------YEEAYDVGVRKYYKAAGQDMEVNYFHAYIDG	383
478	8953571	339	KRGHRVMVVVPRY-------GD-------YEEAYDVGVRKYYKAAGQDMEVNYFHAYIDG	384
479	5825480	339	KRGNRVMVVVPRY-------GD-------YEEAYDVGVRKYYKAAGQDMEVNYFHAYIDG	384
480	7529653	339	KRGHRVMVVVPRY-------GD-------YEEAYDVGVRKYYKAAGQDMEVNYFHAYIDG	384
482	16265834	350	RRGHRVMVVVPRY-------GD-------YAEAQDVGIRKYYKAAGQDLEVKYFHAFIDG	395
525	7489695	31	KRGHRVMVVVPRY-------GD-------YEEAYDVGVRKYYKAAGQDMEVNYFHAYIDG	76
491	15384987	177	RRGHRVMVVIPRY-------GE-------YAEAKDLGVRKRYRVAGQDSEVSYFHAFIDG	222
482	15028467	234	RRGERVMVVIPRY-------GE-------YAEAKDLGVRKRYRVAGQDSEVSYFHAFIDG	279
483	7489711	238	RRGHRVMVVIPRY-------GE-------YAEARDLGVRRRYKVAGQDSEVTYFHSYIDG	283
494	2833384	292	RRGHRVMIVAPRY-------GN-------YAEANDIGVRKRYKVAGQDMEVTYFHTYIDG	337
495	2129898	292	RRGHRVMIVAPHY-------GN-------YAEANDIGVRKRYKVAGQDMEVTYFHTYIDG	337
503	6467503	291	RRGNRVMVVAPRY-------GN-------YVEFQDTGVRKRYKVDGQDFEVSYFQAFIDG	336
496	15232051	332	RRGERVMVVVPRY-------AB-------YAEAKDLGVRKRYKVAGQDMEVMYFHAFIDG	377
497	3192881	170	RRGHRVMVVVPLY-------GN-------YAEPQHTGVRKMFKIDGQDMEXNYFHAYIDN	215
498	2833390	327	RRGHRVMVVAPRY-------DN-------YPEPQDSGVRKIYKVDGQDVDVTYFQALLMD	372
499	14495348	289	RRGHRVMVVVPMY-------KM-------YAEPQQLGEPRRYQVAGQDMEVIYYHAYIDG	334
504	8708896	116	RRGHRVMVVVPRY-------EN-------YDNAWETGIRKIYSVFNSNQEVGYFHAFVDG	161
525	2833387	24	ARGHRVMVVMPRYLNGSSD-KN-------YAKALYTAKHIKIPCFGGSEEVTFFHEYRDN	75
492	15237934	173	GRGHRVMVISPRYLNGTAALKN-------YARAKDLGIRVTVNCFGGSQEVSFYNEYRDG	225
484	5880466	170	ARGHRVMVVMPRYLMGSSD-KN-------YAKALYTGKHIKIPCFGGSHEVTFFHEYRDN	221
505	6690399	96	GRGNRVMVISPRYLNGTAADKN-------YARAKDLGIRVTVNCFGGSQEVSFYHEHRDG	148
485	9369334	170	ARGMRVMVVMPRYLNGSSD-KN-------YAKALYTGKEIKIPCFGGSHEVTFFHEYRDN	221
486	6103327	170	ARGHRVMVVMPRYLNGSSD-KN-------YAKALYTAKHIKIPCFGGSHEVTFFNEYRDN	221
487	7188796	166	ARGHRVMVVMPRYLNGTSD-KN-------YAKALYTGKHIKIPCFGGSHEVTFFEEYRDN	217
506	2829792	162	ARGNRVMVVSPRY-------LNGGPSDEKYANAVDLDVRATVHCFGDAQEVAFYHEYRAG	214
507	12019656	170	ARGNRVMVVMPRYLNGTSD-KN-------YANAFYTEKMIRIPCFGGEHEVTFFHEYRDN	221
493	7489712	163	ARGHRVMVVMPRYLNGTSD-KN-------YANAFYTEKHIRIPCFGGEHEVTFFHEYRDS	214
488	2833377	164	LRGHRVMVVMPRYMNGALN-KN-------FANAFYTEKHIKIPCFGGENEVTFFHEYRDS	215
489	1549232	164	LRGHRVMVVMPRYMNGALN-KN-------FANAFYTEKXIKIPCFGGEHEVTFFMEYRDS	215
490	5295947	164	LRGHRVMVVMPRYMNGALN-KN-------FANAFYTEKHIKIFCFGGEHEVTFFHEYRDS	215
500	6136121	113	GNGHRVMTVSPRY-------DQ-------YKDAWDTSVVVEIKVGDSIETVRFFHCYKRG	158
508	5441242	111	EHGERVMTVSPRY-------DQ-------YKDAWDTNVTVEVKVADRIETVRFFHCYKQG	156
509	2833388	113	ARGHRVMTVSPRY-------DQ-------YKDAWDTSVSVEIKIGDRIETVRFFHSYKRG	158
510	3832512	112	GMGHRVMTVSPRY-------DQ-------YKDAWDTGVSVEIKVGDRFETVRFFWCYKRG	157
511	15223331	115	ARGHRVMTVCPRY-------DQ-------YKDAWDTCVVVQIKVGDKVENVRFFHCYKRG	160
512	2833383	108	GNGHRVMTVSPRY-------DQ-------YKDAWDTNVLVEVKVGDKIETVRFFHCYKRG	153
513	15637079	113	ARGHRVMTVCPRY-------DQ-------YKDAWDTCVVVEIKIGDRIEPVRFFHSYKRG	158
514	267196	112	ARGHRVMTTSPRY-------DQ-------YKDAWDTSVAVEVKVGDSIEIVRFFHCYKRG	157
515	228210	112	ARGHRVNTISPRY-------DQ-------YKDAWDTSVAVEVKVGDSIEIVRFFHCYKRG	157
516	602594	112	ARGHRVMTISPRY-------DQ-------YKDTWDTSVAVEVKVGDSIEIVRFFHCYKRG	157
517	2833381	113	ARGHRVMTVCPRY-------DQ-------YKDAWETCVVVEPQVGDRIEPVRFFHSYKRG	158
518	12003285	109	ANRHRVMTVSPRY-------DQ-------YKDAWDTSVVVEIQVGDKVETVGFFHCYKRG	154
519	15626365	118	ANGHRVMTVTPRY-------DQ-------YKDAWDTNVTIEVKVGDRTEKVRFFHCFKRG	163
520	6624281	108	ANGHRVMVISPRY-------DQ-------YKDAWDTSVISEIKVVDRYERVRYFHCYKRG	153
521	6624287	108	ANGHRVMVISPRY-------DQ-------YKDAWDTSVVSEIKVALEYERVRTFHCYKRG	153
522	4760584	108	ANGNRVMVISPRY-------DQ-------YKDAWDTSVVSEIKVALKYERVRYFHCYKRG	153
523	18139611	112	AMGHRVMTIVPRY-------DQ-------YKDAWDTNVLVEVNIGDRTETVRFFHCYKRG	157
524	17736918	38	ANGHRVMVISPRY-------DQ-------YKDAWDTSVVSEIKVVDKYERVRYFHCYKRG	83
475	Maize SSIIa	318	VDFVFIDAPLFRH---R-----QDDIY----G---G----SR----QEIMK---------	345
476	7489710	318	VDFVFIDAPLFRH---R-----QDDIY----G---G----SR----QEIMK---------	345
477	8953573	384	VDFVFIDAPLFRH---R-----QEDIY----G---G----SR----QEIMK---------	411
478	8953571	385	VDFVFIDAPIFRH---R-----QEDIY----G---G----SR----QEIMK---------	412
479	5825480	385	VDFVFIDAPLFRH---R-----QEDIY----G---G----SR----QEIMK---------	412
480	7529653	385	VDFVFIDAPLFRH---R-----QEDIY----G---G----SR----QEIMK---------	412
481	16265834	396	VDFVFIDAPLFRH---R-----QDDIY----G---G----NR----QEIMK---------	423
502	7489695	77	VDFVFIDAPLFRH---R-----QEDIY----G---G----SR----QEIMK---------	104
491	15384987	223	VDFVFLEAPPFRH---R-----HNDIY----G---G----ER----FDVLK---------	250
482	15028467	280	VDFVFLEAPPFRH---R-----HNDIY----G---G----ER----FDVLK---------	307
483	7489711	284	VDFVFVEAPPFRH---R-----HNNIY----G---G----ER----LDILR---------	311
494	2833384	338	VDIVFIDSPIFPN---L-----ESNIY----G---G----NR----LDILR---------	365
495	2129898	338	VDIVFIDSPIFPN---L-----ESNIY----G---G----NR----LDILR---------	365
503	6467503	337	VDFVFIDSPMFRH---I-----GNDIY----G---G----NR----MDILK---------	364
496	15232051	378	VDFVFIDSPEFRH---L-----SNNIY----G---G----NR----LDILK---------	405
497	3192881	216	VDFVFIDSPIFQH---R-----GNNIY----G---G----NR----VDILK---------	243
498	2833390	373	CDFVFIHSHMFRH---I-----GNNIY----G---G----NR----VDILK---------	400
499	14495348	335	VDFVFIDNPIFEH---V-----ENDIY----G---G----DR----TDILK---------	362
504	8708896	162	VDYVFVDHPTFHG---R-----GKNIY----G---G----ER----QEILF---------	189
488	2833387	76	VDWVFVDHPSY-H---R-----PGSLY----GDNFG----AF----GDNQF---------	105
492	15237934	226	VDWVFVDHKSY-H---R-----PGNPY----G---D----SK----GAFGDNQF------	255
484	5880466	222	VDWVFVDHPSY-H---R-----PGSLY----GDNFG----AF----GDNQF---------	251
505	6690399	149	VDWVFVDHKSY-H---R-----PGNPY----G---D----SK----GAFGDNQF------	178
485	9369334	222	VDWVFVDHPSY-H---R-----PGSLY----GDNFG----AF----GDNQF---------	251
486	6103327	222	VDWVFVDHPSY-H---R-----PGSLY----GDNFG----AF----GDNQF---------	251
487	7188796	218	VDWVFVDHPSY-H---R-----PGSLY----GDNFG----AF----GDNQF---------	247
506	2829792	215	VDWVFVDNSSYCRPGTP-----YGDIY----GAF-G----DN----Q---F---------	244
507	12019656	222	VDWVFVDHPSY-H---R-----PGNLY----GDKFG----AF----GDNQF---------	251
493	7489712	215	VDWVFVDNPSY-H---R----RPGNLY----GDKFG----AF----GDNQF---------	244
488	2833377	216	VDWVFVDHPSY-H---R-----PGNLY----GDNFG----AF----GDNQF---------	245
489	1549232	216	VDWVFVDHPSY-H---R-----PGNLY----GDNFG----AF----GDNQF---------	245
490	5295947	216	VDWVFVDHPSY-N---R-----PGNLY----GDNFG----AF----GDNQF---------	245
500	6136121	159	VDRVFVDHPIFLE---KVWGKTKSKIY----G---P----NAGTDYQDNQL---------	195
508	5441242	157	VDRVFVDHPCFLE---KVWGKTGSKLY----G---P----SAGVDYEDNQL---------	193
509	2833388	159	VDRVFVDHPMFLE---KVWGKTGSKIY----G---PRAGLDY----QDNQL---------	195
510	3832512	158	VDRVFVDHPLFLE---KVWGKTESKLY----G---P----KTGVDYKNQL----------	194
511	15223331	161	VDRVFVDHPIFLA---KVGKTGSKIY----G---PITGVDY----NDNQL----------	197
509	2833383	154	VDRVFVDHPLFLE---RVWGKTGSKLY----G---P----KTGIDYRDNQL---------	190
513	15637079	159	VDRVFVDHPMFLE---KVWGKTGSMLY----G---P----KA----GKDYKDNQL-----	195
514	267196	158	VDRVFVDHPMFLE---K--------VW----G---K----TG----SKIYGPKAGLDYLD	191
515	228210	158	VDRVFVDHPMFLE---K--------VW----G---K----TG----SKIYGPKAGLDYLD	191
516	602594	158	VDRVFVDHPMFLE---K--------VW----G---K----TG----SKIYGPKAGLDYLD	191
517	2833381	159	VDRVFVDHPMFLE---KVWGKTGSMLY----G---P----KA----GKDYKDNQL-----	195
518	12003285	155	VDRVFVDHPLFLE---KVWGKTKSKVY----G---P----SAGVDYEDNQL---------	191
519	15626365	164	VDRVFVDHPIFLE---KVWGKTGTKLY----G---P----AAGDDYQDNQL---------	200
520	6624281	154	VDRVFVDHPCFLE---KVRGKTKEKIYGPDAG---T----DY----EDNQQ---------	190
521	6624287	154	VDRVFVDHPCELE---KVRGKTKEKIY----GPDAG---TD----YEDNQL---------	190
522	4760584	154	VDRVFVDHPCFLE---KVRGKTKEKIYGPDAG---T----DY----EDNQQ---------	190
523	18139611	158	VDRVFVDHPMFLE---KVWGKTGPKLY----G---F----TT----GDDYRDNQL-----	194
524	17736918	84	VDRVFVDHPCFLE---KVRGKTKEKIYGPDAG---T----DY----EDNQQ---------	120
475	(Maize SSIIa)	346	---RMILFCKVAVEVPW-----HVPCGGV-----CYGDG----NLVFIANDWHTALLPVY	388
476	7489710	346	---RMILFCKVAVEVPW-----HVPCGGV-----CYGDG----NLVFIANDWHTALLPVY	388
477	8953573	412	---RMILFCKAAVEVPW-----HVFCGGV-----PYGDG----NLVFIANDWHTALLPVY	454
478	8953571	413	---RMILFCKAAVEVFW-----HVPCGGV-----PYGDG----NLVFIANDWHTALLPVY	455
479	5825480	413	---RMILFCKAAVEVPW-----HVPCGGV-----PYGDG----NLVFIANDWHTALLPVY	455
480	7529653	413	---RMILFCKAAVEVPW-----HVPCGGV-----PYGDG----NLVFIANDWHTALLPVY	455
481	16265834	424	---RMILFCKAAVEVPW-----HVPCGGV-----PYGDG----NLVFLANDWHTALLPVY	466
502	7489695	105	---RMILFCKAAVEVPW-----HVPCGGV-----PYGDG----NLVFIANDWHTALLPVY	147
491	15384987	251	---RMILFCKAAVEVPW-----FAPCGGS-----IYGDG----NLVFIANDWHTALLPVY	293
482	15028467	308	---RMILFCKAAVEVPW-----FAPCGGS-----IYGDG----NLVFIANDWHTALLPVC	350
483	7489711	312	---RMILFCKAAVEVPW-----YAPCGGT-----VYGDG----NLVFIANDWHTALLPVY	354
494	2833384	366	---RMVLFCKAAVEVPW-----HVPCGGI-----CYGDG----NLVFIANDWHTALLPVY	408
495	2129898	366	---RMVLFCKAAVEVPW-----HVPCGGI-----CYGDG----NLVFIANDWHTALLPVY	408
503	6467503	365	---RMVLFCKAAVEVPW-----HVPCGGV-----CYGDG----NLAFIANDWHTALLPVY	407
492	15232051	406	---RMVLFCKAAVEVPW-----YVPCGCV-----CYGDG----NLAFIANDWHTALLPVY	448
497	3192881	244	---RMDLFCKAAIVVPW-----HVPCGGI-----CYGDG----NLVFIANDWNTALLPVY	286
498	2833390	401	---RMVLFCKAAIEVPW-----HVPCGGV-----CYGDG----NLVFIANDWHTALLPAY	443
499	14495348	363	---RMVLLCKAAIEVPW-----YVPCGGY-----CYGDG----NLVFLANDWHTALLPVY	405
504	8708896	190	---RCALLCKAALEAVW-----HVPCGGI-----TYGDD----NLCFIANDWHTALLPVY	232
488	2833387	106	---RYTLLCYAACEAFL-----ILELGGY-----IYGQ-----NCMFVVNDWHASLVPVL	147
492	15237934	256	---RFTLLCHAACEAPL-----VLFLGGF-----TYGEK----SL-FLVNDWHAGLVPIL	297
484	5880466	252	---RYTLLCYAACEAPL-----ILELGGY-----IYGQ-----NCMFVVNDWHASLVPVL	293
505	6690399	179	---RFTLLCHAACEAPL-----VLPLGGF-----TYGEK----SL-FLVNDWHAGLVPIL	220
485	9369334	252	---RYTLLCYAACEAPL-----ILELGGY-----IYGQ-----NCMFVVNDWHASLVPVL	293
486	6103327	252	---RYTLLCYAACEAPL-----ILELGGY-----IYGQ-----NCMFVVNDWHASLVPVL	293
484	7188796	248	---RYTLLCYAACEAPL-----ILELGGY-----IYGQ-----SCMFVVNDWHASLVPVL	289
506	2829792	245	---RFTLLSHAACEAPL-----VLPLGGF-----TYGEK----CL-FLANDWHAPLVPLL	286
507	12019656	252	---RYTLLCYAACEAPL-----VLELGGY-----IYGQ-----NCMFVVNDWHASLVPVL	293
493	7489712	245	---RYTLLCYAACEAPL-----ILELGGY-----IYGQ-----NCMFVVNDWHASLVPVL	286
488	2833377	246	---RYTLLCYAACEAPL-----ILELGGY-----IYGQ-----KCMFVVNDWHASLVPVL	287
489	1549232	246	---RYTLLCYAACEAPL-----ILELGGY-----IYGQ-----KCMFVVNDWHASLVPVL	287
490	5295947	246	---RYTLLCYAACEAPL-----ILEEGGY-----IYGQ-----KCMFVVNDWHASLVPVL	287
500	6136121	196	---RFSLLCQAALEAPR-----VLNLTSS-----KYFSGPYGEDVVFVANDWHTALLPCY	242
508	5441242	194	---RYSLLCQAALEAPR-----VLNLNSN-----KYFSGPYGEDVIFVANDWHTALLPCY	240
509	2833388	196	---RFSLLCLAALEAPR-----VLNLNSSKNFSGPYGE-----EVAFIANDWHTALLPCY	242
510	3832512	195	---RFSLLCQAALEAPR-----VLNLNSN-----KHFSGPYGEDVVFVANDWHTALLPCY	241
511	15223331	198	---RFSLLCQAALEAPQ-----VLNLNSS-----KYFSGPYGEDVVFVANDWHTALLPCY	244
512	2833383	191	---RFSLLCQAALEAPR-----VLNLNSS-----KYFSGPYGEDVIFVANDWHSALIPCY	237
513	15637079	196	---RFSLLCQAALEAPR-----VLNLNSS-----NYFSGPYGEDVVFVANDWHTALLPCY	242
514	267196	192	NELRFSLLCQAALEAPK-----VLNLNSS-----NYFSGPYGEDVLFIANDWHTALIPCY	241
515	228210	192	NELRFSLLCQAALEAPK-----VLNLNSS-----NYFSGPYGEDVLFIANDWHTALIPCY	241
516	602594	192	NELRFSLLCQAALEAPK-----VLNLNSS-----NYFSGPYGEDVLFIANDWHTALIPCY	241
517	2833381	196	---RFSLLCQAALEAPR-----VLNLNSS-----KYFSGPYGEDVVFVANDWHTAWLPCY	242
518	12003285	192	---RFSLLSLAALEAPP-----VLNLTSN-----KYFSGPYGEDVVFVANDWETAVLPCY	238
519	15626365	201	---RFSIFCQAALEAAR-----VLNLKSN-----KYFSGPYGEDVIFVANDWHTALISCY	247
520	6624281	191	---RFSLLCQAALEVPRILDLNNNPHFSG-----PYGE-----DVVFVCNDWHTGLLACY	237
521	6624287	191	---RFSLLCQAALEAPR-----ILDLNNN-----PYFSGPYGEDVVFVCNDWHTGLLACY	237
522	4760584	191	---RFSLLCQAALEVPR-----ILNLDNN-----PYFSGPYGEDVVFVCNDWHTGLLACY	237
523	18139611	195	---RFCLLCLAALEAPR-----VLNLNNS-----EYFSGPYGENVVFVANDWHTGVLPCY	241
524	17736918	121	---RFSLLCQAALEVPR-----ILNLDNN-----PYFSGPYGEDVVFVCNDWHTGLLACY	167
475	Maize SSIIa	389	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	441
476	7489710	389	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	441
477	8953573	455	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	507
478	8953571	456	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	508
479	5825480	456	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	508
480	7529653	456	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	508
481	16265834	467	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	519
502	7489695	148	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	200
491	15384987	294	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	346
482	15028467	351	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	403
483	7489711	355	LKAYYRDHGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQHFELYD----PV---	407
494	2833384	409	LKAYYRDHGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLDLFKMYD----PV---	461
495	2129898	409	LKAYYRDHGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLDLFKMYD----PV---	461
503	6467503	408	LKAYYRDNGLMQYTRSVLVIHNIAHQGRGPSGDFSYVGLPEHYIDLFKLHD----PI---	460
492	15232051	449	LKAYYRDHGIMKYTRSVLVIHNIAHQGRGPVDDFSYVDLPSHYLDSFKLYD----PV---	501
497	3192881	287	LKAYFRDNGVMKFTRSVLVIHNIAHQGRGPMDDFSIVDLPAQUADLFKLYD----PV---	339
498	2833390	444	LKAYYRDNGIMNYTRSVLVIHNIAHQGRGPLEDFSYVDLPPHYMDPFKLYD----PV---	496
499	14495348	406	LKAYYHDNGFMIYARSVLVIHNIAHQGRGPLDDFSYLDLPVDYMDLFKLYD----PF---	458
504	8708896	233	LQAHYRDYGEMTYARCAFVIHNMAHQGRGPFVESEHLELNEEYRERFRLYD----PI---	285
488	2833387	148	LAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	203
492	15237934	298	LAAKYRPYGVYKDARSILIIHNLAHQGVEPAATYTNLGLPS------EWYG----AV---	344
484	5880466	294	LAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	349
505	6690399	221	LAAKYRPYGVYKDARSILIIHNLAHQGVEPAATYTNLGLPS------EWYG----AV---	267
485	9369334	294	LAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	349
486	6103327	294	LAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	349
487	7188796	290	LAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	345
506	2829792	287	LAAKYRPYGVYKDARSIVAIHNIAHQGVEPAVTYNNLGLPPQWYGAVEWIF----PTWAR	342
526	7484400	4	RGRGPFVESEHLELNEEYRERFRLYD----PI---	31
507	12019656	294	LAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	349
493	7489712	287	LAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	342
488	2833377	288	LAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	343
489	1549232	288	LAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	343
490	5295947	288	LAAKYRPYGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVF----PEWAR	343
500	6136121	243	LKSMYQSKGMYLHAKVAFCIHNIAYQGRFGSSDFCLLNLPDQFKSSFDFFDGYEKPV---	299
508	5441242	241	LKSMYQTRGVYRNTKVAFCIHNISYQGRHPFEDFPLLNLPNEYRSAFDFTDGHLKPV---	297
509	2833388	243	LKAIYQPMGIYKHAKVAFCIHNIAYQGRFAFSDFFRLNLPDKFKSSFDFIDGYEKPV---	299
510	3832512	242	LKSLYKSKGIYKSAKVAFCIHNIAYQGPYAFSDLSLLNLPNEFRSSFDFIDGYDKPV---	298
511	15223331	245	LKSMYQSRGVYMNAKVVFCIHNIAYQGRFAFDDYSLLNLPISFKSSFDFMDGYEKPV---	301
512	2833383	238	LKSMYKSRGLYKNAKVAFCIHNIAYQGRNAFSDFSLLNLPDEFRSSFDFIDGYNKPC---	294
513	15637079	243	LKTMYQSRGIYNNAKVAFCIWNIAYQGRFAFSDFSLLNLPDEYKGSFDFIDGYDKPV---	299
514	267196	242	LKSMYQSRGIYLNAKVAFCINNIAYQGRFSFSDFPLLNLPDEFRGSFDFIDGYEKPV---	298
515	228210	242	LKSMYQSRGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRGSFDFIDGYEKPV---	298
516	602594	242	LKSMYQSRGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRGSFDFIDGYEKPV---	298
517	2833381	243	LKTMYQSRGIYMNAKVAFCIHNIAYQGRFAFSDFSLLNLPDEYKGSFDFIDGYDKPV---	299
518	12003285	239	LKTIYQPKGIYTNAKVVLCIHNIAYQGRFAFSDFYKLNLPDQLKSSFDFMDGYEKPV---	295
519	15626365	248	MKSMYQSIGIFRNAKVVFCIHNIAYQGRFAFTDYSLLNLPDQFKSSFDFLDQWIKPI---	304
520	6624281	238	LKSNYQSNGIYRTAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSFDFIDGYDKPV---	294
521	6624287	238	LKSNYQSNGIYMTAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSFDFIDGYDKPV---	294
522	4760584	238	LKSNYQSNGIYRAAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSFDFIDGYDKPV---	294
523	18139611	242	LKSIYQAKGMYThAKVAFCIHNIAYQGRFAREDFELLNLPDSELPSFDFIDGHFKPV---	298
524	17736918	168	LKSNYQSNGIYRAAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSFDFIDGYDKPV---	224

[0394]

TABLE XXI
Maize soluble starch synthase IIa (SSIIa)
“LINKR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)               sequence               end	#
527	MaizeSSIIa	442	----G--------------GEHANIFAAGLKMADRVVTVSRGYLWE-L-KT-VEG-GWG	478
528	7489710	442	----G--------------GEHANIFAAGLKMADRVVTVSRGYLWE-L-KT-VEG-GWG	478
529	8953573	508	----G--------------GEHANYFAAGLKMADQVVTVSPGYLWE-L-KT-VEG-GWG	544
530	8953571	509	----G--------------GEHANYFAAGLKMADQVVVVSPGYLWE-L-KT-VEG-GWG	545
531	5825480	509	----G--------------GEHANYFAAGLKMADQVVVVSPGYLWE-L-KT-VEG-GWG	545
532	7529653	509	----G--------------GEHANYFAAGLKMADQVVVVSPGYLWE-L-KT-VEG-GWG	545
533	16265834	520	----G--------------GEHANIFGAGLKMADRVVTVSPGYLWE-L-KT-TEG-GWG	556
534	7489695	201	----G--------------GEHANYFAAGLKMADQVVTVSPGYLWE-L-KT-VEG-GWG	237
535	15384987	347	----G--------------GEHSNVFAAGLKMADRAVTVSHGYLWE-I-KT-MDG-GWG	383
536	15028467	404	----G--------------GEHSNVFAAGLKMADRAVTVSHGYLWE-I-KT-MDG-GWG	440
537	7489711	408	----G--------------GDHSNVFAAGLKTALRVVTVSNGYMWE-L-KT-SEG-GWG	444
538	2833384	462	----G--------------GEHFNIFAAGLKTADRIVTVSHGYAWE-L-KT-SEG-GWG	498
539	2129898	462	----G--------------GEHFNIFAAGLKTALRIVTVSHGYAWE-L-KT-SEG-GWG	498
540	6467503	461	----G--------------GDHFNIFAPGLKVADRVVTVSHGYAWE-L-KT-SEG-GWG	497
541	15232051	502	----G--------------GEHFNIFAAGLKAADRVLTVSHGYSWE-V-KT-LEG-GWG	538
542	3192881	340	----G--------------GDHFNIFAAGLKTADRVVTVSHGYAWE-L-KT-SEG-GWG	376
543	2833390	497	----G--------------GEHFNIFAAGLKTADRVVTVSHGYSWE-L-KT-SQG-GWG	533
544	14495348	459	----G--------------GDHLNIFAAGIKAADRLLTVSHGYAWE-L-KT-AEG-GWG	495
545	8708896	286	----G--------------GEHMNVMKAGLECAHRLVAVSKCYAWE-C-QT-VEG-GWG	322
546	2833387	204	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGYSWE-V-TT-AEG-GQG	245
547	15237934	345	-----GWVFPTWARTHALDTGEAVNLKGAIVTSDRIITVSQGYAWE-I-TT-VEG-GYG	395
548	5880466	350	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGYSWE-V-TT-AEG-GQG	391
549	6690399	268	-----GWVFPTWARTHALDTGEAVNLKGAIVTSDRIITVSQGYAWE-I-TT-VEG-GYG	318
550	9369334	350	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGY8WE-V-TT-AEG-GQG	391
551	6103327	350	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGYSWE-V-TT-AEG-GQG	391
552	7188796	346	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGYSWE-V-TT-AEG-GQG	387
553	2829792	343	AHALDT-------------GETVNVLKGAIAVADRILTVSQGYSWE-I-TT-PEG-GYG	384
554	7484400	32	----G--------------GEHMNVMKAGLECAHRLVAVSKCYAWE-C-QT-VEG-GWG	68
555	12019656	350	RHALDK-------------GEAVNFLKGAVVTAHPLVTVSKGYSWE-V-TT-AEG-GQG	391
556	7489712	343	RHALDK-------------GEAVNFLKGAVVTADRIVTVSKGYSWE-V-TT-AEG-GQG	384
557	2833377	344	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGYSWE-V-TT-AEG-GQG	385
558	1549232	344	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGYSWE-V-TT-AEG-GQG	385
559	5295947	344	RHALDK-------------GEAVNFLKGAVVTADRIVTVSQGYSWE-V-TT-AEG-GQG	385
560	6136121	300	-----K-------------GRKINWMKAGILESDRVVTVSPYYAME-L-VSGAEK-GVE	337
561	5441242	298	-----R-------------GRKINWMKAAILESDLVLTVSPYYAKE-L-VS-GEDRGVE	335
562	2833388	300	-----K-------------GRKINWMKAGILESDRVLTVSPYYAQEVI-SG-VER-GVE	337
563	3832512	299	-----K-------------GRKINWMKAGVLESDRVFTVSPYYAKE-L-VS-GEDRGVE	336
564	15223331	302	-----K-------------GRKINWMKAAILEAHRVLTVSPYYAQE-L-ISGVDR-GVE	339
565	2833383	295	-----E-------------GKKINWMKAGILESDQVFTVSPHYAKE-L-IS-GEDRGVE	332
566	15637079	300	-----K-------------GRKINWMKAGIREADRVFTVSPNYAKE-L-VSCVSK-GVE	337
567	267196	299	-----K-------------GRKINWMKAGILESHRVVTVSPYYAQE-LVSA-VDK-GVE	336
568	228210	299	-----K-------------GRKINWMKAGILESHRVVTVSPYYAQE-LVSA-VDK-GVE	336
569	602594	299	-----K-------------GRKINWMKAGILESHRVVTVSPYYAQE-L-VSAVDK-GVE	336
570	2833381	300	-----K-------------GRKINWMKAGIREADRVFTVSPNYAKE-L-VSCVSK-GVE	337
571	12003285	296	-----K-------------GRKINWMKAGIIESDRVLTVSPYYAKE-L-VSGPDK-GVE	333
572	15626365	305	-----V-------------GRKINWMkAGIIESHRVLTVSPYYAQE-L-VSGPDK-GVE	342
573	6624281	295	-----E-------------GRKINWMKAGILQADKVLTVSPYYAEE-L-IS-GEARGCG	332
574	6624287	295	-----E-------------GRKINWMKAGILQADKVLTVSPYYAEE-L-IS-GEARGCE	332
575	4760584	295	-----E-------------GRKINWMKAGILQADKVLTVSPYYAEE-L-IS-GEARGCE	332
576	18139611	299	-----V-------------GRKINWMKAGITECDLVMTVSPHYVKE-L-ASGPDK-GVE	336
577	17736918	225	-----E-------------GRKINWMKAGILQADKVLTVSPYYAEE-L-IS-GETRGCE	262
527	Maize SSIIa	479	LHDIIRSNDWKINGIVNGIDHQEWNPKVDVHL-RSDGYTN--YSLETLDAGKRQCKAALQ	535
528	7489710	479	LHDIIRSNDWKINGIVNGIDHQEWNPKVDVHL-RSDGYTN--YSLETLDAGKRQCKAALQ	535
529	8953573	545	LHDIIRQNDWKTRGIVNGIDNMEWNPEVDVHL-KSDGYTN--FSLGTLDSGKRQCKEALQ	601
530	8953571	546	LHDIIRQNDWKTRGIVNGIDNMEWNPEVDVHL-QSDGYTN--FSLSTLDSGKRQCKEALQ	602
531	5825480	546	LHDIIRQNDWKTRGIVNGIDNMEWNPEVDVHL-KSDGYTN--FSLGTLDSGKRQCKEALQ	602
532	7529653	546	LHDIIRQNDWKTRGIVNGIDNMEWNPEVDVHL-KSDGYTN--FSLGTLDSGKRQCKEALQ	602
533	16265834	557	LHDIIRENDWKMNGIVNGIDYREWNPEVDVHL-QSDGYAN--YTVASLDSSKPRCKAALQ	613
534	7489695	238	LHDIIRQNDWKTRGIVNGIDNMEWNPEVDVHL-KSDGYTN--FSLGTLDSGKRQCKEALQ	294
535	15384987	384	LHEIINHNDWKLQGIVNGIDMAEWNPEVDEHL-QSDGYAN--YTFETLDTGKKQCKEALQ	440
536	15028467	441	LHEIINHNDWKLQGIVNGIDMAEWNPEVDEHL-QSDGYAN--YTFETLDTGKKQCKEALQ	497
537	7489711	445	LHDIINQNDWKLQGIVNGIDMSEWNPAVDVHL-HSDDYTN--YTFETLDTGKRQCKAALQ	501
538	2833384	499	LHNIINESDWKFRGIVNGVDTKDWNPQFDAYL-TSDGYTN--YNLKTLQTGKRQCKAALQ	555
539	2129898	499	LHNIINESDWKFRGIVNGVDTKDWNPQFDAYL-TSDGYTN--YNLKTLQTGKRQCKAALQ	555
540	6467503	498	LHNIINENHWKLQGIVNGIDAKEWNPQFDIQL-TSDGYTN--YSLETLDTGKPQCKTALQ	554
541	15232051	539	LHNIINENDWKFRGIVNGIDTQEWNPEFDTYL-HSDDYTN--YSLENLHIGKPQCKAALQ	595
542	3192881	377	LNGIRNENEWKLQGIVNGIDIEEWNPQLDVYL-KSDGYAN--YSLDTLQTGKPQCKAALQ	433
543	2833390	534	LHQIINENDWKLQGIVNGIDTKEWNPELDVHLPRSDGYMN--YSLDTLQTGKPQCKAALQ	591
544	14495348	496	LHGIINESDWKFQGIVNGIDTTDWNPRCDIHL-KSDGYTN--YSLETVQAGKQQCKAALQ	552
545	8708896	323	LHEVIKVNNWKLRGIVNGIDYKEWNPICDEFL-TTDGYAH--YDVDTLAEGKAKCKAALQ	379
546	2833387	246	LNELLSSRKSVLNGIVNGIDINDWNPTTDKCL-PH----H--YSVDDL-SGKAKCKAELQ	297
547	15237934	396	LQDLLSSRKSVINGITNGINVDEWNFSTDEHI-P----FH--YSADDV-SEKIKCKNALQ	447
548	5880466	392	LNELLSSRKSVLNGIVNGIDINDWNPTTDKCL-PH----H--YSVDDL-SGKAKCKAELQ	443
549	6690399	319	LQDLLSSRKSVINGITNGINVDEWNPSTDEHI-P----FH--YSADDV-SEKIKCKAELQ	370
550	9369334	392	LNELLSSRKSVLNGIVNGIDINDWNPTTDKCL-PH----H--YSVDDL-SGKAKCKAELQ	443
551	6103327	392	LNELLSSRKSVLNGIVNGIDINDWNPTTDKCL-PH----H--YSVDDL-SGKAKCKAELQ	443
552	7188796	388	LNELLSSRKSVLNGIVNGIDINDWNPTTDKCL-PH----H--YSVDDL-SGKAKCKAELQ	439
553	2829792	385	LHELLSSRQSVLNGITNGIDVNDWNPSTDEHI-AS----H--YSINDL-SGKVQCKTDLQ	436
554	7484400	69	LHEVIKVNNWKLRGIVNGIDYKEWNPICDEFL-TTDGYAH--YDVDTLAEGKAKCKAALQ	125
555	12019656	392	LNELLSSRKSVLNGIVNGIDINDWNPATDKCI-P----CH--YSVDDL-SGKAKCKGALQ	443
556	7489712	385	LNELLSSRKSVLNGIVNGIDINDWNPATDKCI-P----CH--YSVDDL-SGKAKCKGALQ	436
557	2833377	386	LNELLSSRKSVLNGIVNGIDINDWNPSTDKFL-P----YH--YSVDDL-SGKAKCKAELQ	437
558	1549232	386	LNELLSSRKSVLNGIVNGIDINDWNPSTDKFL-P----YH--YSVDDL-SGKAKCKAELQ	437
559	5295947	386	LNELLSSRKSVLNGIVNGIDINDWNPSTDKFL-P----YH--YSVDDL-SGKAKCKAELQ	437
560	6136121	338	LDNVIAKT--SITGIVNGMDTQEWNPATDKHI-D----TN--YDITTVMDAKPLLKEALQ	388
561	5441242	336	LDNIIRKTG--VAGIVNGMDIREWSPKTDKFI-D----IH--FDTTSVKEAKFLLKEALQ	386
562	2833388	338	LDNFIRKTG--IAGIIHGMDVQEWNPVTDKYI-D----IH--YDATTVMDAKPLLKEALQ	388
563	3832512	337	LDNIIRS--IGITGIVNGMDNREWSPQTDRYI-D----VH--YDASTVTEAKAILKEALQ	387
564	15223331	340	LHKYLRMK--TVSGIINGMDVQEWNPSTDKYI-D----IK--YDITTVTDAKPLIKEALQ	390
565	2833383	333	LDNIIRSTG--IIGIVNGMDNREWSPQTDRYI-D----VH--YNETTVTEAKPLLKGTLQ	383
566	15637079	338	LDNHIR--DCGITGICNGMLTQEWNPATDKYL-A----VK--YDITTVMQAKPLLKEALQ	388
567	267196	337	LDSVLRKT--CITGIVNGMDTQEWNPA-------TDKYTDVKYDITTVMDAKPLLKEALQ	387
568	228210	337	LDSVLRKT--CITGIVNGMDTQEWNPA-------TDKYTDVKYDITTVMDAKPLLKEALQ	387
569	602594	337	LDSVLRKT--CITGIVNGMDTQEWNPA-------TDKYTDVKYDITTVMDAKPLLKEALQ	387
570	2833381	338	LDNHIR--DCGITGICNGMDTQEWNPATDKYL-A----VK--YDITTVMQAKPLLKEALQ	388
571	12003285	334	LDNILRK--CTVTGIVNGMDTQEWNPATDKYI-DN----H--YDITTVMDGKPLLKEALQ	384
572	15626365	343	LDNILRRVG--VTGIVNGMDVQEWNPSTDKYI-S----IK--YDASTVLEGKALLKEELQ	393
573	6624281	333	LDNIMRLTG--ITGIVNGMDVSEWDPIKDKFL-T----VN--YDVTTALEGKALNKEALQ	383
574	6624287	333	LDNIMRLTG--ITGIVNGMLVSEWDPTKDKFL-A----VN--YDVTTALEGKALNKEALQ	383
575	4760584	333	LDNIMRLTG--ITGIVNGMDVSEWDPTKDKFL-A----VN--YDITTALEGKALNKEALQ	383
576	18139611	337	LDGILRTKPLE-TGIVNGMDVYEWNPATDQYI-S----VK--YDATTVTEARALNKEMLQ	388
577	17736918	263	LDNIMRLTG--ITGIVNGNDVSEWDPTKDKFL-A----VN--YDITTALEGKALNKEALQ	313

[0395]

TABLE XXII
Maize soluble starch synthase IIa (SSIIa)
“GLYTR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                Sequence                end	#
578	MaizeSSIIa	536	RELGLEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWI-AG-QDVQLVMLGTGRADLERMLQ	593
579	7489710	536	RELGLEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWI-AG-QDVQLVMLGTGRADLERMLQ	593
580	8953571	602	RELGLQVRGDVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGPHDLEGMLR	659
581	8953571	603	RELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLR	660
582	5825480	603	RELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLR	660
583	7529653	603	RELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLQ	660
584	16265834	614	RELGLERVDDVPLIGFIGRLDGQKGVDIIGDAMPWI-AG-QDVQLVLLGSGRRDLEVMLQ	671
585	7489695	295	RELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLQ	352
586	15384987	441	RQLGLQVRDDVPLIGFIGRLDHQKGVDIIGDAMPWI-AG-QDVQVVMLGTGRPDLEEMLR	498
587	15028467	498	RQLGLQVRDDVPLIGFIGRLDHQKGVDIIGDAMPWI-AG-QDVQVVMLGTGRPDLEEMLR	555
588	7489711	502	RQLGLQVRDDVPLIGFIGRLDHQKGVDIIADAIHWI-AG-QDVQLVMLGTGRADLEDMLR	559
589	2833384	556	RELGLPVREDVPIISFIGRLDHQKGVDLIAEAIPWM-MS-HDVQLVMLGTGRADLEQMLK	613
590	2129898	556	RELGLPVREDVPIISFIGRLDHQKGVDLIAEAIPWM-MS-HDVQLVMLGTGRADLEQMLK	613
591	6467503	555	NELRFAIPPDVPVIGFIGRLDYQKGVDLIAEAIPWM-VG-QDVQLVMLGTGRQDLEEMLR	612
592	15232051	596	KELGLPVRPDVPLIGFIGRLDHQKGVDLIAEAVPWM-MS-QDVQLVMLGTGRPDLEEVLR	653
593	3192881	434	KEMNLPVRDDVPLIGFIGRLDHQKGVDLIAEAIPWM-MG-QDVQLVMLGTGRPDLEQMLK	491
594	2833390	592	KELGLPVRDDVPLIGFIGRLDPQKGVDLIAEAVPWM-MG-QDVQLVMLGTGRPDLEQMLR	649
595	14495348	553	KELGLPVRGDVPVIAFIGRLDHQKGVDLIAEAMPWI-AG-QDVQLIMLGTGRQDLEDTLR	610
596	8708896	380	KELGLPVDPDAPMLGFIGRLDYQKGVDLIRDNYDYI-MG-EKCQLVMLGSGRQDLEDALR	437
597	2833387	298	KELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMR	355
598	15237934	448	KELGLPIRPECPMIGFIGRLDYQKGIDLIQTAGPDL-MV-DDIQFVMLGSGDPKYESWMR	505
599	5880466	444	KELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMR	501
600	6690399	371	KELGLPIRPECPMIGFIGRLDYQKGIDLIQTAGPDL-MV-DDIQFVMLGSGDPKYESWMR	428
601	9369334	444	KELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMR	501
602	6103327	444	KELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMR	501
603	7188796	440	RELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPDL-MR-EDVQFVMLGSGDPVFEGWMR	497
604	2829792	437	KELGLPIRPDCPLIGFIGRLDYQKGVDIILSAIPEL-MQ-NDVQVVMLGSGEKQYEDWMR	494
605	7484400	126	KELGLPVDPDAPMLGFIGRLDYQKGVDLIRDNYDYI-MG-EKCQLVMLGSGRQDLEDALR	183
606	12019656	444	KELGLPIRPEVPLIGFIGRLDYQKGIDLIQLIIPHL-MR-DDVQFVMLGSGDPELEDWMR	501
607	7489712	437	KELGLPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDL-MR-EDVQFVMLGSGDPELEDWMR	494
608	2833377	438	KELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDL-MR-DNIQFVMLGSGDPGFEGWMR	495
609	1549232	438	KELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDL-MR-DNIQFVMLGSGDPGFEGWMR	495
610	5295947	438	KELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDL-MR-DNIQFVMLGSGDPGFEGWMR	495
611	6136121	389	AAVGLPVDKNIPVIGFIGRLEEQKGSDILVAAISKF-VG-LDVQIIILGTGKKKFEQQIQ	446
612	5441242	387	AEVGLPVNRDIPLIGFIGRLEEQKGSDILVEAIPKF-ID-QNVQIIILGTGKKSMEKQIE	444
613	2833388	389	AEVGLPVDRNVPLIGFIGRLEEQKGSDIFVAAISQL-VE-HNVQIVILGTGKKKFEKQIE	446
614	3832512	388	AEVGLPVDRNIPVIGFIGRLEEQKGSDILVESIPKF-ID-QNVQIIVLGTGKKIMEKQIE	445
615	15223331	391	AAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKF-MG-LNVQMVILGTGKKKMEAQIL	448
616	2833383	384	AEIGLPVDSSIPLIGFIGRLEEQKGSDILVEAIAKF-AD-ENVQIVVLGTGKKIMEKQIE	441
617	15637079	389	AAVGLPVDRNIFLIGFIGRLEEQKGSDILYAAISKF-IS-MDVQILILGTGKKKFEQQIE	446
618	297196	388	AAVGLPVDKKIPLIGFIGRLEEQKGSDILVAAIHKF-IG-LDVQIVVLGTGKKEFEQEIE	445
619	228210	388	AAVGLPVDKKIPLIGFIGRLEEQKGSDILVAAIHKF-IG-LDVQIVVLGTGKKEFEQEIE	445
620	602594	388	AAVGLPVDKKVPLIGFIGRLEEQKGSDILVAAIHKF-IG-LDVQIVVLGTGKKEFEQEIE	445
621	2833381	389	AAVGLPVDRNIPLIGFIGRLEEQKGSDILYAAISKF-IS-MDVQILILGTGKKKFEQQIE	446
622	12003285	385	AEVGLPVDP VPLVGFIGRLEEQKGSDILVAALHKF-IE-MDVQVVILGTGKKEFEKQIE	442
623	15626365	394	AEVGLPVDKNVPLIAFIGRLEEQKGSDILVEAIPQF-IK-ENVQIVALGTGKKEMEKQLQ	451
624	6624281	384	AEVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEI-VKEEDVQIVLLGTGKKKFERLLK	442
625	6624287	384	AEVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIVLLGTGKKKFERLLK	442
626	4760584	384	AEVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIVLLGTGKKKFERLLK	442
627	18139611	389	AEVGLPVDSSIPLIVFVGRLEEQKGSDILIAAIPEF-VE-GNVQIIVLGTGKKKMEEELI	446
628	17736918	314	AEVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIVLLGTGKKKFERLLK	372
578	MaizeSSIIa	594	HLEREHPNKVRGWVGFSVPMAHRITAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVG	653
579	7489710	594	HLEREHPNKVRGWVGFSVPMAHRITAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVG	653
580	8953571	660	HFEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVG	719
581	8953571	661	HFEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVG	720
582	5825480	661	HFEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVG	720
583	7529653	661	HFEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVG	720
584	16265834	672	RFEAQHNSKVRGWVGFSVKMAHRITAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVG	731
585	7489695	353	HFEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVG	412
586	15384987	499	RFESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYGTVPVVHAVG	558
587	15028467	556	RFESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYSTVPVVHAVG	615
588	7489711	560	RFESEHSDKVRAWVGFSVPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAVG	619
589	2833384	614	EFEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVG	673
590	2129898	614	EFEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVG	673
591	6467503	613	QFENQHRDKVRGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMMYGTIPVVHAVG	672
592	15232051	654	QMEHQYRDKARGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMNYGTIPVVHAVG	713
593	3192881	492	QIEGQYGDKVRGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMSYGTVPVVHAVG	551
594	2833390	650	QFECQHNDKIRGWVGFSVKTSHRITAGADILLMPSRFEPCALNQLYAMKYGTIPVVHAVG	709
595	14495348	611	RLESQHYDRVRGWVGFSIRLAHRMTAGADILLMPSRFEPCGLNQLYAMMYGTVPVVHAVG	670
596	8708896	438	DMENRNKNQCRGWVGFSNKMAHRITAAADILLMPSRFEPCGLNQLYAMAYGTVPIVHSVG	497
597	2833387	356	STESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	415
598	15237934	506	SMEETYRDKFRGWVGFNVPISHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPVVHGTG	565
599	5880466	502	STESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	561
600	6690399	429	SMEETYRDKFRGWVGFNVPISHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPVVHGTG	488
601	9369334	502	STESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	561
602	6103327	502	STESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	561
603	7188796	498	STESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	557
604	2829792	495	HTENLFKDKFRAWVGFNVPVSHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPIVHSTG	554
605	7484400	184	DMENRNKNQCRGWVGFSNKMAHRITAAADILLMPSRFEPCGLNQLYAMAYGTVPIVHSVG	243
606	12019656	502	STESDFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATG	561
607	7489712	495	STESIFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATG	554
608	2833377	496	STESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	555
609	1549232	496	STESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	555
610	5295947	496	STESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTG	555
611	6136121	447	ELEVLYPDKARGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTIPICASTG	506
612	5441242	445	QLEEIYPEKARGIAKFDGPLAHKIIAGSDFIMIPSRFEPCGLVQLHSMPYGTVPIVSSTG	504
613	2833388	447	HLEVLYPDKARGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPIVASTG	506
614	3832512	446	QLEVTYPGKAIGVAKFNSPLAHKIIAGADFIVIPSRFEPCGLVQLHAMPYGTVPIVSSTG	505
615	15223331	449	ELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSRFEPCGLIQLHAMRYGTVPIVASTG	508
616	2833383	442	VLEEKYPGKAIGITKFNSPLAHKIIAGADFIVIPSRFEPCGLVQLHAMPYGTVPIVSSTG	501
617	15637079	447	QLEVMYPDKARGVAKFNVPLAHMITAGADFMLIPSRFEPCGLIQLHAMRYGTPCICASTG	506
618	297196	446	QLEVLYPNKAKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTG	505
619	228210	446	QLEVLYPGKVKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTG	505
620	602594	446	QLEVLYPNKAKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTG	505
621	2833381	447	QLEVMYPDKARGVAKFNVPLAHMITAGADFMLIPSRFEPCGLIQLHAMRYGTPCICASTG	506
622	12003285	443	QLEELYPGKAVGVAKFNVPLAHKITAGADFMLVPSRFEPCGLIQLHAMRYGTIPICASTG	502
623	15626365	452	QLEISYPDKARGVAKFNVPLAHMMIAGADFILIPSRFEPCGLIQLQAMRYGTVPIVASTG	511
624	6624281	443	SVEEKFPTKVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTG	502
625	6624287	443	SVEEKFPNKVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTG	502
626	4760584	443	SIEEKFPSKVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTG	502
627	18139611	447	LLEVKYPNTARGLAKFNVPLAHMMFAGADFIIVPSRFEPCGLIQLQGMRYGVVPICSSTG	506
628	17736918	373	SIEEKFPSKVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTG	432
578	MaizeSSIIa	654	GLRDTV---------AP-FDPF----GDAGL----G---W--------TFDR---AEANK	681
579	7489710	654	GLRDTV---------AP-FDPF----GDAGL----G---W--------TFDR---AEANK	681
580	8953571	720	GLRDTV---------PP-FDPF----NHSGL----G---W--------TFDR---AEAQK	747
581	8953571	721	GLRDTV---------PP-FDPE----NHSGL----G---W--------TFDR---AEAHK	748
582	5825480	721	GVRDTV---------PP-FDPF----NHSGL----G---W--------TFDR---AEAHK	748
583	7529653	721	GLRDTV---------PP-FDPF----NHSGL----G---W--------TFDR---AEAHK	748
584	16265834	732	GLRDTM---------SA-FDPF----EDTGL----G---W--------TFDR---AEPHK	759
585	7489695	413	GLRDTV---------PP-FDPF----NHSGL----G---W--------TFDR---AEAHK	440
586	15384987	559	GLRDTV---------AP-FDPF----ADTGL----G---W--------TFDR---AEANR	586
587	15028467	616	GLRDTV---------AP-FDPF----ADTGL----G---W--------TFDR---AEANR	643
588	7489711	620	GLRDTV---------AP-FDPF----NDTGL----G---W--------TFDR---AEANR	647
589	2833384	674	GLRDTV---------QP-FNPF----DESGV----G---W--------TFDR---AEANK	701
590	2129898	674	GLRDTV---------QP-FNPF----DESGV----G---W--------TFDR---AEANK	701
591	6467503	673	GLRDTV---------QP-FDPF----NESGL----G---W--------TFDS---AESHK	700
592	15232051	714	GLRDTV---------QQ-FDPY----SETGL----G---W--------TFDS---AEAGK	741
593	3192881	552	GLRDTV---------QP-FDPF----NESGY----G---W--------TFGR---ARANQ	579
594	2833390	710	GLRDTV---------QP-FDPL----MSQDW----G---G--------PSDR---AEASQ	737
595	14495348	671	GLRDTV---------EH-YNPY----EESGL----G---W--------TFEK---AEANR	698
596	8708896	498	GLRDTV---------KQ-YSPF----ENVGT----G---W--------VFER---AEANK	525
597	2833387	416	GLRDTV---------ET-FNPFGAK-GEEGT----G---W--------AFSP---LTVDK	446
598	15237934	566	GLRDTV---------EN-FNPYAEGGAGTGT----G---W--------VFTP---LSKDS	597
599	5880466	562	GLRDTV---------ET-FNPFGAK-GEEGT----G---W--------AFSP---LTVDK	592
600	6690399	489	GLRDTV---------EN-FNFYAEGGAGAGT----c---W--------VFTP---LSKDS	520
601	9369334	562	GLRDTV---------ET-FNPFGAK-GEEGT----G---W--------AFSP---LTVDK	592
602	6103327	562	GLRDTV---------ET-FNPFGAK-GEEGT----G---W--------AFSP---LTVDK	592
603	7188796	558	GLRDTV---------ET-FNPFGAK-GEEGT----G---W--------AFSP---LTVEK	588
604	2829792	555	GLRDTV---------KD-FNPY----AQEGIGEGTG---W--------TFSP---LTSEK	586
605	7484400	244	GLRDTV---------KQ-YSPF----ENVGT----G---W--------VFER---AEANK	271
606	12019656	562	GLRDTV---------EN-FNPFGEN-GEQGT----G---W--------AFAP---LTTEN	592
607	7489712	555	GLRDTV---------EN-FNPFGEN-GEQGT----G---W--------AFAP---LTTEN	585
608	2833377	556	GLRDTV---------EN-FNPFAEK-GEQGT----G---W--------AF	579
609	1549232	556	GLRDTV---------EN-FNPFAEK-GEQGT----G---W--------AF	579
610	5295947	556	GLRDTV---------EN-FNPFAEK-GEQGT----G---W--------AF	579
611	6136121	507	GLVDTV---------TEGFTGF----HMGAF----NVECA--------TVDP---ADVQK	538
612	5441242	505	GLVDTV---------QEGFTGF----HMGAF----NVDCE--------AIDP---ADVEK	536
613	2833388	507	GLVDTV---------KEGYTGF----QMGAL----H---V--------ECDKIDSADVAA	538
614	3832512	506	GLVDTV---------KEGYTGF----HVGAF----SVECE--------AVDP---ADVEK	537
615	15223331	509	GLVDTV---------KDGYTGF----HIGRF----N---V--------KCEV---VDPDD	537
616	2833383	502	GLVDTVKEGYTGFHAGP-FDVE----CE--------------------DVDP---DDVDK	533
617	15637079	507	GLVDTV---------KEGYTGF----HMGAF----NVDCE--------TVDP---EDVLK	538
618	297196	506	GLVDTV---------KEGYTGF----HMGAF----N---VECD-----VVDP---ADVLK	537
619	228210	506	GLVDTV---------KEGYTGF----HMGAF----N---VECD-----VVDP---ADVLK	537
620	602594	506	GLVDTV---------KEGYTGF----HMGAF----N---VECD-----VVDP---ADVLK	537
621	2833381	507	GLVDTV---------KEGYTGF----HMGAF----NVDCE--------TVDP---EDVLK	538
622	12003285	503	GLVDTV---------KEGFTGF----HMGAF----N---VECD-----AVDP---ADVLK	534
623	15626365	512	GLVDTV---------KEGFTGF----HMGSF----N---V--------KCDA---VDPVD	540
624	6624281	503	GLVDTI---------VE---------GKTGF----H---MGRLSVDCNVVEP---ADVKK	534
625	6624287	503	GLVDTI---------VE---------GKTGF----H---MGRLSVDCNVVEP---ADVKK	534
626	4760584	503	GLVDTI---------VE---------GKTGF----H---MGRLSVDCNVVEP---ADVKK	534
627	18139611	507	GLVDTV---------KE-GVTG----FHMGLFNVEC---E--------TVDP---VDVTA	538
628	17736918	433	GLVDTI---------VE---------GKTGF----H---MGRLSVDCNVVEP---ADVKK	464

[0396]

TABLE XXIII
Maize soluble starch synthase IIa (SSIIa)
“GLYTR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)              Sequence              end	#
629	MaizeSSIIa	682	LIEALRHCLDTYRKYGES-WKSLQARGMSQDLSWDHAAELYEDVLVKAKYQW	732
630	7489710	682	LIEALRHCLDTYRKYGES-WKSLQARGMSQDLSWDHAAELYEDVLVKAKYQW	732
631	8953571	748	LIEALGHCLRTYRDYKES-WRGLQERGMSQDFSWEHAAKLYEDVLVKAKYQW	798
632	8953571	749	LIEALGHCLRTYRDYKES-WRGLQERGMSQDFSWEHAAKLYEDVLLKAKYQW	799
633	5825480	749	LIEALGHCLRTYRDYKES-WRGLQERGMSQDFSWEHAAKLYEDVLLKAKYQW	799
634	7529653	749	LIEALGHCLRTYRDFKES-WRALQERGMSQDFSWEHAAKLYEDVLVKAKYQW	799
635	16265834	760	LIEALGHCLETYRKYKES-WRGLQVRGMSQDLSWDHAAELYEEVLVKAKYQW	810
636	7489695	441	LIEALGHCLRTYRDFKES-WRALQERGMSQDFSWEHAAKLYEDVLVKAKYQW	491
637	15384987	587	MIDALGHCLNTYRNYKES-WRGLQARGMAQDLSWDHAAELYEDVLVKAKYQW	637
638	15028467	644	MIDALGHCLNTYRNYKES-WRGLQARGMAQDLSWDHAAELYEDVLVKAKYQW	694
639	7489711	648	MIDALSHCLTTYRNYKES-WRACRARGMAEDLSWDHAAVLYEDVLVKAKYQW	698
640	2833384	702	LMAALWNCLLTYKDYKKS-WEGIQERGMSQDLSWDNAAQQYEEVLVAAKYQW	752
641	2129898	702	LMAALWNCLLTYKDYKKS-WEGIQERGMSQDLSWDNAAQQYEEVLVAAKYQW	752
642	6467503	701	LIHALGNCLLTYREYKKS-WEGLQRRGMTPNLSWDHAAEKYEETLVAAKYQW	751
643	15232051	742	LIHALGNCLLTYREYKES-WEGLQRRGMTQDLSWDNAAEKYEEVLVAAKYHW	792
644	3192881	580	LIDALGNCLLTYRQYKQS-WEGLQRRGMMQDLSWDHAAEKYEEVLVAAKYQW	630
645	2833390	738	LIPRIRNCLLTYREYKKS-WEGIQTRCMTQDLSWDNAAQNYEEVLIAAKYQW	788
646	14495348	699	LIDALGHCLNTYRNYRTS-WEGLQKRGMMQDLSWDNAAKLYEEVLLAAKYQW	749
647	8708896	526	LRESINNALYTYRQFRDS-FRGIQRRGMEQDLTWDNAASIYEEVLVAAKYQW	576
648	2833387	447	MLWALRTAMSTFRENKPS-WEGLMKRGMTKDHTWDHA	482
649	15237934	598	MVSALRLAAATYREYKQS-WEGLMRRGMTRNYSWENAAVQYEQV-----FQW	643
650	5880466	593	MLWALRTAMSTFREHKPS-WEGLMKRGMTKDHTWDHAAEQYEQI	635
651	6690399	521	MVSALRLAAATYREYKQS-WEGLMRRGMTRNYSWENAAVQYEQV-----FQW	566
652	9369334	593	MLWALRTAMSTFREHKPS-WEGLMKRGMTKDHTWDHAAEQYEQI	635
653	6103327	593	MLWALRTAMSTFREHKPS-WEGLMKRGMTKDHTWDHAAEQYEQI	635
654	7188796	589	MLWALRTAISTFREHKPS-WEGLMKRGMTKDHTWDHAAEQYEQI	631
655	2829792	587	LLDTLKLAIGTYTEHKSS-WEGLMRRGMGRDYSWENAAIQYEQVFTWA	633
656	7484400	272	LRESINNALYTYRQFRDS-FRGIQRRGMEQDLTWDNAASIYEEVLVAAKYQW	322
657	12019656	593	MFVDIANC	600
658	7489712	586	MFVDIANC	593
659	6136121	539	IATTVERALAAYGSV--A-YKEMIQNCMAQDLSWKGPAKNWEKMLL	581
660	5441242	537	IATTVRRALGTYGTV--A-MEKIIQNCMAQDFSWKGPAKQWEKVL	578
661	2833388	539	IVKTVARALGTYAT--AA-LREMILNCMAQDLSWKGPARMWEKMLL	581
662	3832512	538	LATTVNRALKTYGT--QA-LKEMILNCMAQDFSWKGPAKQWEQALL	580
663	15223331	538	VIATAKAVTRAVAVYGTSAMQEMVKNCMDQDFSWKGPARLWEKVLL	583
664	2833383	534	LAATVKRALKTYGT--QA-MKQIILNCMAQNFSWKKPAKIWEKALL	576
665	15637079	539	VITTVGRALAMYGTL--A-FTEMIKNCMSQELSWKGPAKNWETVLL	581
666	297196	538	IVTTVARALAVYGTL--A-FAEMIKNCMSEELSWKEPAKKWETLLL	580
667	228210	538	IVTTVARALAVYGTL--A-FAEMIKNCMSEELSWKEPAKKWETLLL	580
668	602594	538	IVTTVARALAVYGTL--A-FAEMIKNCMSEELSWKEPAKKWETLLL	580
669	2833381	539	VITTVGRALAIYGTL--A-FTEMIKNCMSQELSWKGPAKNWETVLL	581
670	12003285	535	IVKTVGRALEVYGT--PA-FREMINNCMSLDLSWKGPAKNWETVLL	577
671	15626365	541	VDAIPKTVTKALGVYGTSAFAEMIKNCMAQELSWKGPAKYWEEVLL	586
672	6624281	535	VVTTLKRAVKVVGT--PA-YHEMVKNCMIQDLSWKGPAKNWEDVLLE	578
673	6624287	535	VVTTLKRAVKVVGT--PA-YHGMVKNCMIQDLSWKGPAKNWEDVLLE	578
674	4760584	535	VVTTLKRAVKVVGT--PA-YHEMVKNCMIQDLSWKGPAKNWEDVLLE	578
675	18139611	539	VASTVKRALKQYNT--PA-FQEMVQNCMAQDLSWKGPAKKWEEVLL	581
676	17736918	465	VVTTLKRAVKVVGT--PA-YHEMVKNCMIQDLSWKGPAKNWEDVLLE	508

[0397]

TABLE XXIV
Identities of the Accession Numbers used in Tables. XIX-XXIV.
Accession	Brief Description of sequences	score
Id.	producing significant alignments	(bits)	E-value
gi|7489710|pir| |T01208	ADPglucose--starch glucosyltransfera . . .	1389	0.0
gi|8953573|emb|CAB96627.1 (AJ269504)	starch synthase IIa-3 . . .	976	0.0
gi|8953571|emb|CAB96626.1 (AJ269503)	starch synthase IIa-2 . . .	976	0.0
gi|5825480|gb|AAD53263.1|AF155217_1 (AF155217)	starch synth . . .	975	0.0
gi|7529653|emb|CAB86618.1| (AJ269502)	starch synthase IIa-1 . . .	970	0.0
gi|16265834|gb|AAL16661.1|AF419099_1 (AF419099)	putative so . . .	968	0.0
gi|7489695|pir| |T06798	probable starch synthase (EC 2.4.1.- . . .	910	0.0
gi|15384987|emb|CAC59826.1| (AJ308110)	soluble starch synth . . .	902	0.0
gi|15028467|gb|AAK81729.1|AF395537_1 (AF395537)	soluble sta . . .	892	0.0
gi|7489711|pir| |T01209	ADPglucose--starch glucosyltransfera . . .	863	0.0
gi|2833384|sp|Q43093|UGS3 PEA	Glycogen [starch] synthase, c . . .	836	0.0
gi|2129898|pir| |S61505	UDPglucose--starch glucosyltransfera . . .	835	0.0
gi|6467503|gb|AAF13168.1|AF173900_1 (AF173900)	granule boun . . .	832	0.0
gi|115232051|ref|(NP 186767.1| (NM_110984)	putative glycogen . . .	826	0.0
gi|3192881|gb|AAC19119.1| (AF068834)	starch synthase [Ipomo . . .	813	0.0
gi|2833390|sp|Q43847|UGS3 SOLTU	Glycogen [starch] synthase, . . .	788	0.0
gi|14495348|gb|AAK64284.1|AF383878_1 (AF383878)	soluble sta . . .	786	0.0
gi|8708896|gb|AAC17970.21 (AF026421)	soluble starch synthas . . .	692	0.0
gi|2833387|sp|Q43654|UGS2 WHEAT	Soluble glycogen [starch] s . . .	448	e−125
gi|15237934|ref|NP 197818.1| (NM_122336)	soluble starch syn . . .	446	e−124
gi|5880466|gb|AAd54661.1| (AF091803)	starch synthase I [Tri . . .	444	e−123
gi|6690399|gb|AAF24126.1|AF121673_1 (AF121673)	soluble star . . .	444	e−123
gi|9369334|emb|CAB99209.1| (AJ292521)	starch synthase I-1 [ . . .	444	e−123
gi|6103327|gb|AAF03557.1| (AF091802)	starch synthase I [Aeg . . .	442	e−123
gi|7188796|gb|AAF37876.1|AF234163_1 (AF234163)	starch synth . . .	441	e−122
gi|2829792|sp|P93568|UGS2 SOLTU	Soluble glycogen [starch] s . . .	440	e−122
gi|7484400|pir| |T07924	probable starch synthase (EC 2.4.1.- . . .	428	e−119
gi|12019656|gb|AAd45815.2| (AF168786)	soluble starch syntha . . .	412	e−114
gi|7489712|pir| |T01414	AdPglucose--starch glucosyltransfera . . .	407	e−112
gi|2833377|sp|Q40739|UGS2 ORYSA	Soluble glycogen [starch] s . . .	391	e−107
gi|1549232|dbj|BAA07396.1| (D38221)	SSS1 [Oryza sativa] >gi . . .	390	e−107
gi|5295947|dbj|BAA81848.1| (AB026295)	ESTs AU075322 (C11109) . . .	390	e−107
gi|6136121|sp|082627|UGST ANTMA	Granule-bound glycogen [sta . . .	350	3e−95
gi|5441242|dbj|BAA82346.1| (AB029546)	granule-bound starch . . .	344	2e−93
gi|2833388|sp|Q43784|UGST MANES	Granule-bound glycogen [sta . . .	342	9e−93
gi|3832512|gb|AAC70779.1| (AF097922)	granule-bound glycogen . . .	338	9e−92
gi|15223331|ref|Np_174566.1| (NM_103023)	starch synthase, p . . .	337	4e−91
gi|2833383|sp|Q43092|UGST PEA	Granule-bound glycogen [starc . . .	334	2e−90
gi|15637079|dbj|BA968126.1| (AB071604)	granule-bound starch . . .	333	5e−90
gi|2671961|sp|Q00775|UST SOLTU	Granule-bound glycogen [star . . .	332	1e−89
gi|228210|prf| |11718316A	granule-bound starch synthase [Sola . . .	330	2e−89
gi|602594|emb|CAA58220.1| (X83220)	starch (bacterial glycog . . .	330	2e−89
gi|2833381|sp|Q42857|UGST IPOBA	Granule-bound glycogen [sta . . .	327	2e−88
gi|12003285|9b|AAG43519.1|AP210699_1 (AF210699)	granule-bou . . .	327	2e−88
gi|15626365|emb|CAC69955.1| (AJ345045)	granule-bound starch . . .	327	3e−88
gi|6624281|dbj|BAA88509.1| (AB029061)	starch synthase (GBSS . . .	320	4e−86
gi|6624287|dbj|BAA88512.1| (A3029064)	starch synthase (GBSS . . .	319	7e−86
gi|4760584|dbj|BAA77352.1| (AB019624)	starch synthase (GBSS . . .	318	1e−85
gi|18139611|gb|AAL58572.1| (AY069940)	granule binding starc . . .	318	1e−85
gi|17736918|gb|AA41028.1| (AF2S0137)	mutant granule bound . . .	318	1e−85
gi|6318538|gb|AAF06936.1|AF110373_1 (AF110373)	granule-boun . . .	318	2e−85
gi|6624285|dbj|BAA88511.1| (AB029063)	starch synthase (GBSS . . .	317	4e−85
gi|6318540|gb|AAF06937.1|AF110374_1 (AF110374)	granule-boun . . .	316	5e−85
gi|4760582|dbj|BAA77351.1| (AB019623)	starch synthase (GBSS . . .	316	5e−85
gi|11037536|gb|AAG27624.1|AF286320_1 (AF286320)	granule bou . . .	316	6e−85
gi|136765|sp|P27736|UGST WHEAT	Granule-bound glycogen [star . . .	314	2e−84
gi|4760580|dbj|BAA77350.1| (AB019622)	starch synthase (GESS . . .	313	4e−84
gi|136755|sp|P09842|UGST HORVU	Granule-bound glycogen [star . . .	313	4e−84
gi|18652407|gb|AAL77109.1|AF474373_6	(AF474373) granule-bon . . .	313	5e−84
gi|2833385|sp|Q43134|UGST SORBI	Granule-bound glycogen [sta . . .	313	5e−84
gi|6492245|gb|AAF14233.1|AF109395_1 (AF109395)	granule-boun . . .	312	8e−84
gi|6624283|dbj|BAA88510.11 (AB029062)	starch synthase (GBSS . . .	311	1e−83
gi|4588609|gb|AAD26156.1|AF113844_1 (AF113844)	granule-boun . . .	311	2e−83
gi|16716335|gb|AAC17969.3| (AF026420)	granule-bound starch . . .	309	7e−83
gi|82478|pir| |JQ0703	UDPglucose--starch glucosyltransferasa . . .	308	9e−83
gi|2833382|sp|Q42968|UGST ORYGL	Granule-bound glycogen [sta . . .	307	2e−82
gi|297424|emb|CAA46294.1|(X65183)	glycogen (starch) syntha . . .	306	5e−82
gi|136757|sp|P047l3 UGST MAIZE	Granule-bound glycogen [star . . .	306	5e−82
gi|7798551|gb|AAC61675.2| (AF031162)	granule-bound starch s . . .	306	7e−82
gi|136758|sp|P19395|UGST ORYSA	Granule-bound glycogen [star . . .	305	8e−82
gi|297422|emb|CAA45472.1| (X64108)	starch granule-hound sta . . .	301	2e−80
gi|4588607|gb|AAD26155.1|AF113843_1 (AF113843)	granule-boun . . .	293	4e−78
gi|15643657|ref|NP_228703.1| (NC_000853)	glycogen synthase . . .	284	2e−75
gi|15900991|ref|NP_345595.1| (NC_003028)	glycogen synthase . . .	283	4e−75
gi|15903076|ref|NP_358626.1| (NC_003098)	Glycogen synthase . . .	283	5e−75
gi|15672681|ref|NP_266855.1| (NC_002662)	glycogen synthase . . .	273	4e−72
gi|17366711|sp|Q9CEM9|GLGA LACLA	Glycogen synthase (Starch . . .	272	8e−72
gi|18309046|ref|NP_560980.1| (NC_003366)	glycogen synthase . . .	264	2e−69
gi|16080147|ref|NP_390973.1| (NC_000964)	starch (bacterial . . .	263	6e−69
gi|15895507|ref|NP_348856.1| (NC_003030)	Glycogen synthase, . . .	261	2e−68
gi|2811062|sp|008328|GLGA BACST	Glycogen synthase (Starch [ . . .	257	2e−67
gi|17229371|ref|NP_485919.1| (NC_003272)	glycogen synthase . . .	251	2e−65
gi|15613648|ref|NP_241951.1| (NC_002570)	starch (bacterial . . .	248	1e−64
gi|1641730|ref|NP_231362.1| (NC_002505)	glycogen synthase . . .	234	2e−60
gi|16766821|ref|NP_462436.1| (NC_003197)	glycogen synthase . . .	233	5e−60
gi|16331219|ref|NP_441947.1| (NC_000911)	glycogen synthase . . .	231	2e−59
gi|15620537|gb|AAL03921.1|U30252_9 (U30252)	GlgA [Synechoco . . .	231	3e−59
gi|16762766|ref|NP_458383.1| (NC_003198)	glycogen synthase . . .	231	3e−59
gi|17938870|ref|NP_535658.1| (NC_003306)	glycogen synthase . . .	222	1e−56
gi|16119514|ref|NP_396220.1| (NC_003064)	AGR_pAT_410p [Agro . . .	221	1e−56
gi|16124067|ref|NP_407380.1| (NC_003143)	glycogen synthase . . .	219	6e−56
gi|6116748|dbj|BAA85761.1| (AB028026)	granule-bound starch . . .	219	7e−56
gi|15803938||ref NP_289974.1| (NC_002655)	glycogen synthase . . .	219	1e−55
gi|15966599|ref|NP_386952.1| (NC_003047)	PROBABLE GLYCOGEN . . .	217	4e−55
gi|15890897|ref|NP_356569.1| (NC_003063)	AGR_L_1562p [Agrob . . .	216	8e−55
gi|13476305|ref|NP_107875.1| (NC_002678)	glycogen synthase . . .	215	2e−54
gi|14279432|gb|AAK58595.1|AF268959_2 (AF268969)	glycogen sy . . .	214	4e−54
gi|17366749|sp|Q9EUT5|GLGA_RHITR	Glycogen synthase (Starch . . .	213	5e−54
gi|15717885|gb|AAK97773.1| (AY044844)	starch synthase isofo . . .	213	7e−54
gi|4582783|emb|CAB40375.1| (AJ006752)	starch synthase, isof . . .	213	7e−54
gi|16329217|ref|NP_439945.1| (NC_000911)	glycogen (starch) . . .	211	2e−53
gi|17227527|ref|NP_484075.1| (NC_003272)	glycogen (starch) . . .	211	3e−53
gi|9587293|gb|AAF89248.1|AF285973_1 (A9285973)	granule-boun . . .	209	8e−53
gi|9587321|gb|AAF89262.1|AF285987_1 (AF285987)	granule-boun . . .	209	1e−52
gi|8901183|gb|AAC17971.2| (AF026422)	soluble starch synthas . . .	208	1e−52
gi|15602409|ref|NP_245481.1| (NC_002663)	GlgA [Pasteurella . . .	208	2e−52
gi|9587317|gb|AAF89260.1|AF285985_1 (AF285985)	granule-boun . . .	207	5e−52
gi|9587301|gb|AAF89252.1|AF285977_1 (AF285977)	granule-boun . . .	206	8e−52
gi|15605118|ref|NP_213495.1| (NC_000918)	glycogen synthase . . .	205	2e−51
gi|146139|gb|AAA23870.1| (J02616)	glycogen synthase (EC 2.4 . . .	204	2e−51
gi|16265159|ref|NP_437951.1| (NC_003078)	putative glycogen . . .	204	2e−51
gi|9587329|gb|AAF89266.1|AF28599_1 (AF285991)	granula-boun . . .	204	3e−51
gi|9587352|gb|AAF89276.1|AF286003_1 (AF286003)	granula-boun . . .	203	7e−51
gi|9587311|gb|AAF89257.1|AF285982_1 (AF285982)	granule-boun . . .	199	1e−49
gi|9587305|gb|AAF89254.1|AF285979 1 (AF285979)	granule-boun . . .	198	1e−49
gi|9587343|gb|AAF89273.1|A9285998 1 (AF285998)	granule-boun . . .	198	2e−49
gi|17548463|ref|NP_521803.1| (NC_003296)	PROBABLE GLYCOGEN . . .	198	2e−49
gi|18461221|dbj|BAb84418.1| (AP003292)	putative starch synt . . .	197	2e−49
gi|9587337|gb|AAF89270.1|AF285995_1 (AF285995)	granule-boun . . .	197	3e−49
gi|16273270|ref|NP_439511.1| (NC_000907)	glycogen synthase . . .	196	5e−49
gi|9587348|gb|AAF89274.1| (AF286001)	granule-bound starch s . . .	196	5e−49
gi|9587341|gb|AAF89272.1|AF285997 1(AF285997)	granule-boun . . .	196	6e−49
gi|9587323|gb|AAF89263.1|AF285988 1(AF285988)	granule-boun . . .	196	7e−49
gi|15236819|ref|NP_193558.1| (NM 117934)	starch synthase-li . . .	194	2e−48
gi|9587307|gb|AA989255.1|AF285980_1 (AF285980)	granule-boun . . .	194	2e−48
gi|9587327|gb|AAF89265.1|AF285990_1 (AF285990)	granule-boun . . .	194	3e−48
gi|9587319|gb|AAF89261.1|AF285986_1 (AF285986)	granule-boun . . .	194	4e−48
gi|15983795|gb|AAL10494.1| (AY056803)	Atlg32900/F9L11_8 [Ar . . .	193	5e−48
gi|9587313|gb|AAF89258.1|AF285983_1 (AF285983)	granule-boun . . .	193	7e−48
gi|9587297|gb|AAF89250.1|AF285975_1 (AR285975)	granule-boun . . .	193	7e−48
gi|9587309|gb|AAF89256.1|AF285981_1 (AF285981)	granule-boun . . .	192	8e−48
gi|9587299|gb|AAF89251.1|AF285976_1 (AF285976)	granule-boun . . .	192	8e−48
gi|9587333|gb|AAF89268.1|AF285993_1 (AF285993)	granule-boun . . .	192	9e−48
gi|9587325|gb|AAF89264.1|AF285989_1 (AF285989)	granule-boun . . .	191	2e−47
gi|5834407|gb|AAD53959.1|AF181035_3 (AF181035)	glycogen syn . . .	191	3e−47
gi|17367070|sp|Q9RNH6|GLGA RHOSH	Glycogen synthase (Starch . . .	191	3e−47
gi|15221083|ref|NP_172637.1| (NM_101044)	putative glycogen . . .	190	4e−47
gi|15597361|ref|NP_250855.1| (NC_002516)	probable glycogen . . .	189	1e−46
gi|9587331|gb|AAF89267.1|AF285992_1 (AF285992)	granule-boun . . .	189	1e−46
gi|7489826|pir| |T01265	starch synthase DULL1 - maize >gi|30 . . .	188	2e−46
gi|9587303|gb|AAF89253.1|AF285978_1 (AF285978)	granule-boun . . .	188	2e−46
gi|4582789|emb|CAB40374.1| (AJ225088)	Starch synthase isofo . . .	188	2e−46
gi|9587339|gb|AAF89271.1|AF285996_1 (AP285996)	granula-boun . . .	187	4e−46
gi|9587335|gb|AAF89269.1|AF285994_1 (AF285994)	granule-boun . . .	187	4e−46
gi|9587295|gb|AAF89249.1|AF285974_1 (AF285974)	granule-boun . . .	186	6e−46
gi|15805621|ref|NP_294317.1| (NC_001263)	glycogen synthase . . .	184	4e−45
gi|17367076|sp|Q9RWS1|GLGA DEIRA	Glycogen synthase (Starch . . .	183	6e−45
gi|7489274|pir| |T07663	soluble starch synthase (EC 2.4.1.-) . . .	183	7e−45
gi|2833389|sp|Q43846|UGS4 SOLTU	Soluble glycogen [starch]s . . .	181	3e−44
gi|9502145|gb|AAF88000.1| (AF258609)	starch synthase III [A . . .	179	8e−44
gi|9502143|gb|AAF87999.1|AF258608_1 (AF258608)	starch synth . . .	177	2e−43
gi|7484399|pir| |T07926	probable starch synthase (EC 2.4.1.- . . .	177	3e−43
gi|17366350|sp|P58395|GLGA THECA	Glycogen synthase (Starch . . .	176	5e−43
gi|17646328|gb|AAL40942.1 AF432915_1 (AF432915)	putative st . . .	175	1e−42
gi|12278503|gb|AAG48992.1| (AY011005)	granule-bound starch . . .	170	5e−41
gi|3493007|gb|AAD02961.1| (AF079241)	granule-bound starch s . . .	170	6e−41
gi|12278507|gb|AAG48994.1| (AY011007)	granule-bound starch . . .	170	6e−41
gi|3493065|gb|AAD02990.1| (AF079270)	granule-bound starch s . . .	169	8e−41
gi|3493055|gb|AAD02985.1| (AF079265)	granule-bound starch s . . .	169	1e−40
gi|3493079|gb|AAD02997.1| (AF079277)	granule-bound starch s . . .	169	1e−40
gi|12278417|gb|AAG48949.1| (AY010962)	granule-bound starch . . .	169	1e−40
gi|3493057|gb|AAD02986.1| (AF079266)	granule-bound starch s . . .	169	1e−40
gi|3493061|gb|AAD02988.1| (AF079268)	granule-bound starch s . . .	169	1e−40
gi|12278505|gb|AAG48993.1| (AY011006)	granule-bound starch . . .	169	1e−40
gi|3493059|gb|AAD02987.1| (AF079267)	granule-bound starch s . . .	169	1e−40
gi|3493107|gb|AAD03011.1| (AF079291)	granule-bound starch s . . .	168	2e−40
gi|3493049|gb|AAD02982.1| (AF079262)	granule-bound starch s . . .	168	2e−40
gi|12278487|gb|AAG48984.1| (AY010997)	granule-bound starch . . .	168	2e−40
gi|3493089|gb|AAD03002.1| (AF079282)	granule-bound starch . . .	168	2e−40
gi|12278473|gb|AAG48977.1| (AY010990)	granule-bound starch . . .	168	2e−40
gi|3493051|gb|AAD02983.1| (AF079263)	granule-bound starch s . . .	167	2e−40
gi|12278427|gb|AAG48954.1| (AY010967)	granule-bound starch . . .	167	3e−40
gi|12278497|gb|AAG48989.1| (AY011002)	granule-bound starch . . .	167	3e−40
gi|3493005|gb|AAD02960.1| (AF079240)	granule-bound starch s . . .	167	3e−40
gi|12278463|gb|AAG48972.1| (AY010985)	granule-bound starch . . .	167	3e−40
gi|12278509|gb|AAG48995.1| (AYO11008)	granule-bound starch . . .	167	3e−40
gi|3493093|gb|AAD03004.1| (AF079284)	granule-bound starch s . . .	167	3e−40
gi|3493073|gb|AAD02994.1| (AF079274)	granule-bound starch s . . .	167	4e−40
gi|12278471|gb|AAG48976.1| (AY010989)	granule-bound starch . . .	167	4e−40
gi|3493019|gb|AAD02967.1| (AF079247)	granule-bound starch s . . .	167	5e−40
gi|12278453|gb|AAG48967.1| (AY010980)	granule-bound starch . . .	167	5e−40
gi|12278491|gb|AAG48986.1| (AY010999)	granule-bound starch . . .	166	6e−40
gi|12278451|gb|AAG48966.1| (AY010979)	granule-bound starch . . .	166	6e−40
gi|3493077|gb|AAD02996.1| (AF079276)	granule-bound starch s . . .	166	7e−40
gi|3493067|gb|AAD02991.1| (AF079271)	granule-bound starch s . . .	166	7e−40
gi|3493097|gb|AAD03006.1| (AF079286)	granule-bound starch s . . .	166	7e−40
gi|12278514|gb|AAG48997.1| (AY011011)	granule-bound starch . . .	166	7e−40
gi|3493121|gb|AAD03018.1| (AF079298)	granule-bound starch s . . .	166	8e−40
gi|3493091|gb|AAD03003.1| (AF079283)	granule-bound starch s . . .	166	8e−40
gi|12278481|gb|AAG48981.1| (AY010994)	granule-bound starch . . .	166	8e−40
gi|3493083|gb|AAD02999.1| (AF079279)	granule-bound starch s . . .	166	8e−40
gi|3493111|gb|AAD03013.1| (AF079293)	granule-bound starch s . . .	166	8e−40
gi|12278457|gb|AAG48969.1| (AY010982)	granule-bound starch . . .	166	9e−40
gi|13774482|gb|AAK38880.1| (AF353518)	granule-bound starch . . .	166	9e−40
gi|12278479|gb|AAG48980.1| (AY010993)	granule-bound starch . . .	166	9e−40
gi|3493095|gb|AAD03005.1| (AF079285)	granule-bound starch s . . .	166	1e−39
gi|3493081|gb|AAD02998.1| (AF079278)	granule-bound starch s . . .	166	1e−39
gi|112278429|gb|AA048955.1| (AY010968)	granule-bound starch . . .	166	1e−39
gi|3493087|gb|AAD03001.1| (AF079281)	granule-bound starch s . . .	166	1e−39
gi|12278485|gb|AAG48983.1| (AY010996)	granule-bound starch . . .	166	1e−39
gi|3493025|gb|AAD02970.1| (AF079250)	granule-bound starch s . . .	165	1e−39
gi|13774486|gb|AAK38882.1| (AF353520)	granule-bound starch . . .	165	1e−39
gi|3493117|gb|AAD03016.1| (AF079296)	granule-bound starch s . . .	165	1e−39
gi|12278425|gb|AAG48953.1| (AY010966)	granule-bound starch . . .	165	2e−39
gi|3493031|gb|AAD02973.1| (AF079253)	granule-bound starch s . . .	164	2e−39
gi|12278499|gb|AAG48990.1| (AY011003)	granule-bound starch . . .	164	3e−39
gi|12278443|gb|AAG48962.1| (AY010975)	granule-bound starch . . .	164	3e−39
gi|12278511|gb|AAG48996.1| (AY011009)	granule-bound starch . . .	164	3e−39
gi|12278495|gb|AAG48988.1| (AY011001)	granule-bound starch . . .	164	3e−39
gi|3493099|gb|AAD03007.1| (AF079287)	granule-bound starch s . . .	164	3e−39
gi|12278411|gb|AAG48946.1| (AY010959)	granule-bound starch . . .	164	3e−39
gi|13377473|gb|AAK20725.1| (AF318769)	granule-bound starch . . .	164	3e−39
gi|12278501|gb|AAG48991.1| (AY011004)	granule-bound starch . . .	164	3e−39
gi|12278449|gb|AAG48965.1| (AY010978)	granule-bound starch . . .	164	4e−39
gi|3493103|gb|AAD03009.1| (AF079289)	granule-bound starch s . . .	164	4e−39
gi|12278477|gb|AAG48979.1| (AY010992)	granule-bound starch . . .	164	4e−39
gi|12278423|gb|AAG48952.1| (AY010965)	granule-bound starch . . .	164	4e−39
gi|122784211|gb|AAG48951.1| (AY010964)	granule-bound starch . . .	164	4e−39
gi|12278489|gb|AAG48985.1| (AY010998)	granule-bound starch . . .	164	4e−39
gi|13774484|gb|AAK38881.1| (AF353519)	granule-bound starch . . .	164	4e−39
gi|3493023|gb|AAD02969.1| (AF079249)	granule-bound starch s . . .	164	4e−39
gi|13377475|gb|AAK20726.1| (AF318770)	granule-bound starch . . .	164	4e−39
gi|12278435|gb|AAG48985.1| (AY010971)	granule-bound starch . . .	164	4e−39
gi|12278469|gb|AAG48975.1| (AY010988)	granule-bound starch . . .	163	5e−39
gi|3493101|gb|AAD03008.1| (AF079288)	granule-bound starch s . . .	163	5e−39
gi|3493113|gb|AAD03014.1| (AF079294)	granule-bound starch s . . .	163	5e−39
gi|12278419|gb|AAg48950.1| (AY010963)	granule-bound starch . . .	163	6e−39
gi|3493071|gb|AAD02993.1| (AF079273)	granule-bound starch s . . .	163	6e−39
gi|3493053|gb|AAD02984.1| (AF079264)	granule-bound starch s . . .	163	6e−39
gi|3493075|gb|AAD02995.1| (AF079275)	granule-bound starch s . . .	163	7e−39
gi|12278475|gb|AAG48978.1| (AY010991)	granule-bound starch . . .	162	8e−39
gi|3493041|gb|AAG42978.1| (AF079258)	granule-bound starch s . . .	162	9e−39
gi|12278433|gb|AAG48957.1| (AY010970)	granule-bound starch . . .	162	9e−39
gi|3493037|gb|AAD02976.1| (AF079256)	granule-bound starch s . . .	162	1e−38
gi|3493017|gb|AAD02966.1| (AF079246)	granule-bound starch s . . .	162	1e−38
gi|3493015|gb|AAD02965.1| (AF079245)	granule-bound starch s . . .	162	1e−38
gi|12278437|gb|AAG48959.1| (AY010972)	granule-bound starch . . .	162	1e−38
gi|3493021|gb|AAD02968.1| (AF079248)	granule-bound starch s . . .	162	1e−38
gi|3493069|gb|AAD02992.1| (AF079272)	granule-hound starch s . . .	162	1e−38
gi|3493027|gb|AAD02971.1| (AF079251)	granule-bound starch s . . .	161	2e−38
gi|3493013|gb|AAD02964.1| (AF079244)	granule-bound starch s . . .	161	2e−38
gi|3493011|gb|AAD02963.1| (AF079243)	granule-bound starch s . . .	161	2e−38
gi|12278493|gb|AAG48987.1| (AY011000)	granule-bound starch . . .	161	2e−38
gi|12278413|gb|AAG48947.1| (AY010960)	granule-bound starch . . .	161	2e−38
gi|3493085|gb|AAD03000.1| (AF079280)	granule-bound starch s . . .	161	3e−38
gi|3493009|gb|AAD02962.1| (AF079242)	granule-bound starch s . . .	161	3e−38
gi|3493035|gb|AAD02975.1| (AF079255)	granule-bound starch s . . .	160	4e−38
gi|3493063|gb|AAD02989.1| (AF079269)	granule-bound starch s . . .	160	4e−38
gi|12278431|gb|AAG48956.1| (AY010969)	granule-bound starch . . .	160	4e−38
gi|3492999|gb|AAD02957.1| (AF079237)	granule-bound starch s . . .	160	5e−38

[0398]

SSIIb
Accession: AF019297
NID: g2655030
Mol. wt. (calc) = 75458      Residues = 698
SEQ.ID. No. 677
1   M P G A I S S S S S A F L L P V A S S S P R R R R G S V G A
31  A L R S Y G Y S G A E L R L H W A R R G P P Q D G A A S V R
61  A A A A P A G G E S E E A A K S S S S S Q A G A V Q G S T A
91  K A V D S A S P P N P L T S A P K Q S Q S A A M Q N G T S G
121 G S S A S T A A P V S G P K A D H P S A P V T K R E I D A S
151 A V K P E P A G D D A R P V E S I G I A E P V D A K A D A A
181 P A T D A A A S A P Y D R E D N E P G P L A G P N V M N V V
211 V V A S E C A P F C K T G G L C D V V G A L P K A L A R R G
241 H R V M V V I P R Y G E Y A E A R D L G V R R R Y K V A G Q
271 D S E V T Y F H S Y I D G V D F V F V E A P P F R H R H N N
301 I Y C G E R L D I L K R M I L F C K A A V E V P W Y A P C G
331 G T V Y G D G N L V F I A N D W H T A L L P V Y L K A Y Y R
361 D N G L M Q Y A R S V L V I H N I A H Q G R G P V D D F V N
391 F D L P E H Y I D H F K L Y D N I G G D H S N V F A A G L K
421 T A D R V V T V S N G Y M W E L K T S E G G W G L H D I I N
451 Q N D W K L Q G I V N G I D M S E W N P A V D V H L H S D D
481 Y T N Y T F E T L D T G K R Q C K A A L Q R Q L G L Q V R D
511 D V P L I G F I G R L D H Q K G V D I I A D A I H W I A G Q
541 D V Q L V M L G T G R A D L E D M L R R F E S E H S D K V R
571 A W V C F S V P L A H R I T A G A D I L L M P S R F E P C G
601 L N Q L Y A M A Y G T V P V V H A V G G L R D T V A P F D P
631 F N D T G L G W T F D R A E A N R M I D A L S H C L T T Y R
661 N Y K E S W R A C R A R G M A E D L S W D H A A V L Y E D V
691 L V K A K Y Q W

[0399]

TABLE XXV
Maize soluble starch synthase IIb (SSIIb)
Alignments with other similar proteins-Transit Peptide
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                  Sequence                  ending	#
678	MAIZE SSIIb	1	MPGAISSSSSAFL-LXXXXXXXXXXXXXXXXALRSYGYSGAELRLHWARRGPPQDXXXXX	59
679	7489711	1	MPGAISSSSSAFL-LPVASSSPRRRRGSVGAALRSYGYSGAELRLHWARRGPPQDGAASV	59
680	15028467	1	MSGAIASSPAATLFLAGSSSSSPRRRRSRVSGVWWHLYGGTGLRLHWERRGLVRDGAVVC	60
Cont . . . d
678	Maize SSIIb	61	XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKAVDSASPPNPLTSAPKQSQSA-AMQNXX	118
679	7489711	60	RAAAAPAGGESEEAAKSSSSSQAGAVQGSTAKAVDSASPPNPLTSAPKQSQSA-AMQNGT	118
680	15028467	61	SASAAGGEDGVAKAKTKSAGSSKAVAVQGSTAKADHVEDS---VSSPKYVKPAVAKQNGE	117
681	15384987	45	SSPKSVKPAVAKQNGE	60
Cont . . . d
678	Maize SSIIb	119	XXXXXXXXXXPVSGPKADHPSAPVTKREID--ASAVKPEPAGDDARPVESIGIXXXXXXX	176
679	7489711	119	SGGSSASTAAPVSGPKADHPSAPVTKREID--ASAVKPEPAGDDARPVESIGIAEPVDAK	176
680	15028467	118	VVSRATKSDAPVSKPKVD-PSVPASKAEADGNAQAVESKAALDKK---EDVGVAEPLEAK	173
681	15384987	61	VVSRATKSDAPVPKPKVD-PSVPASKAEADGNAQAVESKAALDKK---EDVGVAEPLEAK	116

[0400]

TABLE XXVI
Maize soluble starch synthase IIb (SSIIb)
“GLASS” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start                   Sequence                   end	#
682	MaizeSSIIb	177	XXXXXXXXXXXXXXYDREDNEPGPLAGPNV-----MNVVVVASECAPFCKTGGLGDVVGA	231
683	7489711	177	ADAAPATDAAASAPYDREDNEPGPLAGPNV-----MNVVVVASECAPFCKTGGLGDVVGA	231
684	15028467	174	ADAGGDAGAVSSAD-DSENKESGPLAGPNV-----MNVIVVASECSPFCKTGGLGDVVGA	227
685	15384987	117	ADAGGDAGAVSSAD-DSENKESGPLAGPNV-----MNVIVVASECSPFCKTGGLGDVVGA	170
686	7489710	229	DNDSGPLAGENV-----MNVIVVAAECSPWCKTGGLGDVVGA	265
687	16265834	306	QDDDSGPLAGENV-----MNVIVVAAECSPWCKTGGLGDVAGA	343
688	15232051	287	KDEEKPPPLAGANV-----MNVILVAAECAPFSKTGGLGDVAGA	325
689	7489695	1	NVVVVAAECSPWCKTGGLGDVAGA	24
690	6467503	247	EDMKPPPLAGDNV-----MNVILVAAECAPWSKTGGLGDVAGS	284
691	2833384	245	FESGGEKPPPLAGTNV-----MNIILVSAECAPWSKTGGLGDVAGS	285
692	2129898	245	FESGGEKPPPLAGTNV-----MNIILVSAECAPWSKTGGLGDVAGS	285
693	3192881	126	EDLKPPPLAGTNV-----MNVILVCAECAPWSKTGGLGDVAGA	163
694	8953571	294	QNHDSGPLAGENV-----MNVVVVAAECSPWCKTGGLGDVAGA	331
695	7529653	295	QNHDSGPLAGENV-----MNVVVVAAECSPWCKTGGLGDVAGA	332
696	8953571	295	QNHDSGPLAGENV-----MNVVVVAAECSPWCKTGGLGDVAGA	332
697	5825480	295	QNHDSGPLAGENV-----MNVVVVAAECSPWCKTGGLGDVAGA	332
698	14495348	247	DDPSASASVDL-----INIILVAAECAPWSKTGGLGDVAGA	282
699	2833390	284	DEKPPPLAGTNV-----MNIILVASECAPWSKTGGLGDVAGA	320
700	8708896	75	KPPPLAGPNV-----MNVVMVGAECAPWSKTGGLGDVMAA	109
701	5880466	140	SIVFVTGEAAPYAKSGGLGDVCGS	163
702	9369334	140	SIVFVTGEAAPYAKSGGLGDVCGS	163
703	15237934	141	V-----NNLVFVTSEAAPYSKTGGLGDVCGS	166
704	7188796	136	SIVFVTGEAAPYAKSGGLGDVCGS	159
705	6103327	140	SIVFVTGEAAPYAKSGGLGDVCGS	163
706	9369336	140	SIVFVTGEAAPYAKSGGLGDVCGS	163
707	6690399	64	V-----NNLVFVTSEAAPYSKTGGLGDVCGS	89
708	2829792	118	DTEEMEETPIK--LT-----FNIIFVTAEAAPYSKTGGLGDVCGS	155
709	2833387	1	EAAPYAKSGGLGDVCGS	17
710	12019656	140	SIVFVTGEASPYAKSGGLGDVCGS	163
711	7489712	133	SIVFVTGEASPYAKSGGLGDVCGS	156
712	5295947	134	SVVFVTGEASPYAKSGGLGDVCGS	157
713	1549232	134	SVVFVTGEASPYAKSGGLGDVCGS	157
714	2833377	134	SVVFVTGEASPYAKSGGLGDVCGS	157
715	6136121	82	-----MNLVFVLAEVGPWSKTGGLGDVVGG	106
716	228210	81	-----MNLIFVGTEVGPWSKTGGLGDVLGG	105
717	297196	81	-----MNLIFVGTEVGPWSKTGGLGDVLGG	105
718	2833388	82	-----MNLIFVGAEVGPWSKTGGLGDVLGG	106
719	15637079	65	ENEGGMAAGTIVCKQQGMNLVFVGCEVGPWCKTGGLGDVLGG	106
720	602594	81	-----MNLIFVGTEVGPWSKTGGLGDVLGG	105
721	2833381	82	-----MNLVFVGCEEGPWCKTGGLGDVLGG	106
722	5441242	80	-----MNLIFVGAEVAPWSKTGGLGDVLGG	104
723	15223331	84	-----MSVIFIGAEVGPWSKTGGLGDVLGG	108
724	12003285	78	-----MTLIFVSAECGPWSKTGGLGDVVGG	102
725	3832512	79	NG-----MNLVFVGAEVGPWSKTGGLGDVLGG	105
726	15626365	87	-----MNLIFVGTEVAPWSKTGGLGDVLGG	111
727	2833383	77	-----MSLVFVGAEVGPWSKTGGLGDVLGG	101
728	6624281	73	GSGG-----MNLVFVGAEMAPWSKTGGLGDVLGG	101
729	17736918	3	GSGG-----MNLVFVGAEMAPWSKTGGLGDVLGG	31
730	4760584	73	GSGG-----MNLVFVGAEMAPWSKTGGLGDVLGG	101
731	6492245	63	PAPIVCSTG-----MPIIFVATEVHPWCKTGGLGDVVGG	96
682	MaizeSSIIb	232	LPKALARRGHRVMVVIPRY-------GEYAEARDLG----VRRRYKVA--GQDSEVTYFH	278
683	7489711	232	LPKALARRGHRVMVVIPRY-------GEYAEARDLG----VRRRYKVA--GQDSEVTYFH	278
684	15028467	228	LPKALARRGHRVMVVIPRY-------GEYAEAKDLG----VRKRYRVA--GQDSEVSYFH	274
685	15384987	171	LPKALARRGHRVMVVIPRY-------GEYAEAKDLG----VRKRYRVA--GQDSEVSYFH	217
686	7489710	266	LPKALARRGHRVMVVVPRY-------GDYVEAFDMG----IRKYYKAA--GQDLEVNYFH	312
687	16265834	344	LPKALARRGHRVMVVVPRY-------GDYAEAQDVG----IRKYYKAA--GQDLEVKYFH	390
688	15232051	326	LPKSLARRGNRVMVVVPRY-------AEYAEAKDLG----VRKRYKVA--GQDMEVMYFH	372
689	7489695	25	LPKALAKRGHRVMVVVPRY-------GDYEEAYDVG----VRKYYKAA--GQDMEVNYFH	71
690	6467503	285	LPKALARRGHRVMVVAPRY-------GNYVEPQDTG----VRKRYKVD--GQDFEVSYFQ	331
691	2833384	286	LPKALARRGHRVMIVAPHY-------GNYAEAHDIG----VRKRYKVA--GQDMEVTYFH	332
692	2129898	286	LPKALARRGHRVMIVAPHY-------GNYAEAHDIG----VRKRYKVA--GQDMEVTYFH	332
693	3192881	164	LPKALARRGHRVMVVVPLY-------GNYAEPQHTG----VRKMFKID--GQDMEVNYFH	210
694	8953571	332	LPKALAKRGHRVMVVVPRY-------GDYEEAYDVG----VRKYYKAA--GQDMEVNYFH	378
695	7529653	333	LPKALAKRGHRVMVVVPRY-------GDYEEAYDVG----VRKYYKAA--GQDMEVNYFH	379
696	8953571	333	LPKALAKRGHRVMVVVPRY-------GDYEEAYDVG----VRKYYKAA--GQDMEVNYFH	379
697	5825480	333	LPKALAKRGHRVMVVVPRY-------GDYEEAYDVG----VRKYYKAA--GQDMEVNYFH	379
698	14495348	283	LPKALARRGURVMVVVPMY-------KNYAEPQQLG----EPRRYQVA--GQDMEVIYYH	329
699	2833390	321	LFKALARRGHRVMVVAPRY-------DNYPEPQDSG----VRKIYKVD--GQDVDVTYFQ	367
700	8708896	110	LPKALVRRGHRVMVVVPRY-------ENYDNAWETG----IRKIYSVF--NSNQEVGYFH	156
701	5880466	164	LPIALAARGHRVMVVMPRYLNGSSD-KNYAKALYTG----KHIKIPCF--GGSHEVTFFH	216
702	9369334	164	LPIALAARGHRVMVVMPRYLNGSSD-KNYAKALYTG----KHIKIPCF--GGSHEVTFFH	216
703	15237934	167	LPIALAGRGHRVMVISPRYLNGTAADKNYARAKDLG----IRVTVNCF--GGSQEVSFYH	220
704	7188796	160	LPIALAARGHRVMVVMPRYLNGTSD-KNYAKALYTG----KHIKIPCF--GGSHEVTFFH	212
705	6103327	164	LPIALAARGHRVMVVMPRYLNGSSD-KNYAKALYTA----KHIKIPCF--GGSHEVTFFH	216
706	9369336	164	LPIALAARGHRVMVVMPRYLNGSSD-KNYAKALYTA----YHIKIPCF--GGSHEVTFFH	216
707	6690399	90	LPIALAGRGHRVMVISPRYLNGTAADKNYAPAKDLG----IRVTVNCF--GGSQEVSFYH	143
708	2829792	156	LPMALAARGHRVMVVSPRYLNGGPSDEKYANAVDLD----VRATVHCF--GDAQEVAFYH	209
709	2833387	18	LPIALAARGHRVMVVMPRYLNGSSD-KNYAKALYTA----KHIKIPCF--GGSHEVTFFH	70
710	12019656	164	LPVALAARGHRVMVVMPRYLNGTSD-KNYANAFYTE----KHIRIPCF--GGEHEVTFFH	216
711	7489712	157	LPVALAARGHRVMVVMPRYLNGTSD-KNYANAFYTE----KHIRIPCF--GGEHEVTFFH	209
712	5295947	158	LPIALALRGHRVMVVMPRY-------MNGALNKNFANAFYTEKHIKIPCFGGEHEVTFFH	210
713	1549232	158	LPIALALRGHRVMVVMPRY-------MNGALNKNFANAFYTEKHIKIPCFGGEHEVTFFH	210
714	2833377	158	LPIALALRGHRVMVVMPRY-------MNGALNKNFANAFYTEKHIKIPCFGGEUEVTFFH	210
715	6136121	107	LPPAMAGNGHRVMTVSPRY-------DQYKDAWDTS----VVVEIKVG--DSIETVRFFH	153
716	228210	106	LPPALAARGHRVMTISPRY-------DQYKDAWDTS----VAVEVKVG--DSIEIVRFFH	152
717	297196	106	LPPALAARGHRVMTISPRY-------DQYKDAWDTS----VAVEVKVG--DSIEIVRFFH	152
718	2833388	107	LPPAMAARGHRVMTVSPRY-------DQYKDAWDTS----VSVEIKIG--DRIETVRFFH	153
719	15637079	107	LPPALAARGHRVMTVCPRY-------DQYKDAWDTC----VVVELQVG--DRIEPVRFFH	153
720	602594	106	LPPALAARGHRVMTISPRY-------DQYKDTWDTS----VAVEVKVG--DSIEIVRFFH	152
721	2833381	107	LPPALAARGHRVMTVCPRY-------DQYKDAWETC----VVVEPQVG--DRIEPVRFFH	153
722	5441242	105	LPSALAEHGHRVMTVSPRY-------DQYKDAWDTN----VTVEVKVA--DRIETVRFFH	151
723	15223331	109	LPPALAARGHRVMTICPRY-------DQYKDAWDTC----VVVQIKVG--DKVENVRFFH	155
724	12003285	103	LPPALAANRHRVMTVSPRY-------DQYKDAWDTS----VVVEIQVG--DKVETVGFFH	149
725	3832512	106	LPPALAGNGHRVMTVSPRY-------DQYKDAWDTG----VSVEIKVG--DRFETVRFFH	152
726	15626365	112	LPPALSANGHRVMTVTPRY-------DQYKDAWDTN----VTIEVKVG--DRTEKVRFFH	158
727	2833383	102	LPPVLAGNGHRVMTVSPRY-------DQYKDAWDTN----VLVEVKVG--DKIETVRFFH	148
728	6624281	102	LPAAMAANGHRVMVISPRY-------DQYKDAWDTS----VISEIKVV--DRYERVRYFH	148
729	17736918	32	LPPAMAANGHRVMVISPRY-------DQYKDAWDTS----VVSEIKVV--DKYERVRYFH	78
730	4760584	102	LPPAMAANGHRVMVISPRY-------DQYKDAWDTS----VVSEIKVV--DKYERVRYFH	148
731	6492245	97	LPPALAAMGHRVMTIAPRY-------DQYKDTWDTN----VLVEVIVG--DRTETVRFFH	143
682	MaizeSSIIb	279	SYIDGVDFVFVEAPPFRH---R---------HNNIY----G---G----ERLDILKRMIL	315
683	7489711	279	SYIDGVDFVFVEAPPFRH---R---------HNNIY----G---G----ERLDILKRMIL	315
684	15028467	275	AFIDGVDFVFLEAPPFRH---R---------HNDIY----G---G----ERFDVLKRMIL	311
685	15384987	218	AFIDGVDFVFLEAPPFRH---R---------HNDIY----G---G----ERFDVLKRMIL	254
686	7489710	313	AFIDGVDFVFIDAPLFRH---R---------QDDIY----G---G----SRQEIMKRMIL	349
687	16265834	391	AFIDGVDFVFIDAPLFRH---R---------QDDIY----G---G----NRQEIMKRMIL	427
688	15232051	373	AFIDGVDFVFIDSPEFRH---L---------SNNIY----G---G----NRLDILKRMVL	409
689	7489695	72	AYIDGVDFVFIDAPLFRH---R---------QEDIY----G---G----SRQEIMKRMIL	108
690	6467503	332	AFIDGVDFVFIDSPMFRH---I---------GNDIY----G---G----NRMDILKRMVL	368
691	2833384	333	TYIDGVDIVFIDSPIFRN---L---------ESNIY----G---G----NRLDILRRMVL	369
692	2129898	333	TYIDGVDIVFIDSPIFRN---L---------ESNIY----G---G----NRLDILRRMVL	369
693	3192881	211	AYIDNVDFVFIDSPIFQH---R---------GNNIY----G---G----NRVDILKRMDL	247
694	8953571	379	AYIDGVDFVFIDAPLFRH---R---------QEDIY----G---G----SRQEIMKRMIL	415
695	7529653	380	AYIDGVDFVFIDAPLFRH---R---------QEDIY----G---G----SRQEIMKRMIL	416
696	8953571	380	AYIDGVDFVFIDAPIFRH---R---------QEDIY----G---G----SRQEIMKRMIL	416
697	5825480	380	AYIDGVDFVFIDAPLFRH---R---------QEDIY----G---G----SRQEIMKRMIL	416
698	14495348	330	AYIDGVDFVFIDNPIFHH---V---------ENDIY----G---G----DRTDILKRMVL	366
699	2833390	368	ALLMDCDFVFIHSHMFRH---I---------GNNIY----G---G----NRVDILKRMVL	404
700	8708896	157	AFVDGVDYVFVDHPTFHG---R---------GKNIY----G---G----ERQEILFRCAL	193
701	5880466	217	EYRDNVDWVFVDHPSY-H---R---------PGSLY----GDNFG----AFGDNQFRYTL	255
702	9369334	217	EYRDNVDWVFVDHPSY-H---R---------PGSLY----GDNFG----AFGDNQFRYTL	255
703	15237934	221	EYRDGVDWVFVDHKSY-H---R---------PGNPY----GDSKG----AFGDNQFRFTL	259
704	7188796	213	EYRDNVDWVFVDHPSY-H---R---------PGSLY----GDNFG----AFGDNQFRYTL	251
705	6103327	217	EYRDNVDWVFVDHPSY-H---R---------PGSLY----GDNFG----AFGDNQFRYTL	255
706	9369336	217	EYRDNVDWVFVDHPSY-H---R---------PGSLY----GDNFG----AFGDNQFRYTL	255
707	6690399	144	EHRDGVDWVFVDHKSY-H---R---------PGNPY----GDSKG----AFGDNQFRFTL	182
708	2829792	210	EYRAGVDWVFVDHSSYCRPGTP---------YGDIY----G---A----FG-DNQFRFTL	248
709	2833387	71	EYRDNVDWVFVDUPSY-H---R---------PGSLY----GDNFG----AFGDNQFRYTL	109
710	12019656	217	EYRDSVDWVFVDHPSY-H---R---------PGNLY----GDKFG----AFGDNQFRYTL	255
711	7489712	210	EYRDSVDWVFVDNPSY-H---R---------PGNLY----GDKFG----AFGDNQFRYTL	248
712	5295947	211	EYRDSVDWVFVDHPSY-H---R---------PGNLY----GDNFG----AFGDNQFRYTL	249
713	1549232	211	EYRDSVDWVFVDNPSY-H---R---------PGNLY----GDNFC----AFGDNQFRYTL	249
714	2833377	211	EYRDSVDWVFVDHPSY-H---R---------PGNLY----GDNFG----AFGDNQFRYTL	249
715	6136121	154	CYKRGVDRVFVDHPIFLE---KVWGKT----KSKIYGPNAG---T----DYQDNQLRFSL	199
716	228210	153	CYKRGVDRVFVDHPMFLE---KVWGKT----GSKIY----G---PKAGLDYLDNELRFSL	198
717	297196	153	CYKRGVDRVFVDHPMFLE---KVWGKT----GSKIY----G---PKAGLDYLDNELRFSL	198
718	2833388	154	SYKRGVDRVFVDHPMFLE---KVWGKT----GSKIY----G---PRAGLDYQDNQLRFSL	199
719	15637079	154	SYKRGVDRVFVDHPMFLE---KVWGKTGSMLYGPKA----G---K----DYKDNQLRFSL	199
720	602594	153	CYKRGVDRVFVDHPMFLE---KVWGKT----GSKIY----G---PKAGLDYLDNELRFSL	198
721	2833381	154	SYKRGVDRVFVDHPMFLE---KVWGKTGSMLYGPKA----G---K----DYKDNQLRFSL	199
722	5441242	152	CYKQGVDRVFVDHPCFLE---KVWGKT----GSKLY----G---PSAGVDYEDNQLRYSL	197
723	15223331	156	CYKRGVDRVFVDHPIFLA---KVVGKTCSKIYGPIT----G---V----DYNDNQLRFSL	201
724	12003285	150	CYKRGVDRVFVDHPLFLE---KVWGKT----KSKVY----G---PSAGVDYEDNQLRFSL	195
725	3832512	153	CYKRGVDRVFVDHPLFLE---KVWGKT----ESKLY----G---PKTGVDYKDNQLRFSL	198
726	15626365	159	CFKRGVDRVFVDHPIFLE---KVWGKTGTKLYGPAA----G---D----DYQDNQLRFSI	204
727	2833383	149	CYKRGVDRVFVDHPLFLE---RVWGKT----GSKLY----G---PKTGIDYRDNQLRFSL	194
728	6624281	149	CYKRGVDRVFVDHPCFLE---KVRGKT----KEKIYGPDAG---T----DYEDNQQRFSL	194
729	17736918	79	CYKRGVDRVFVDHPCFLE---KVRGKT----KEKIYGPDAG---T----DYEDNQQRFSL	124
730	4760584	149	CYKRGVDRVFVDHPCFLE---KVRGKT----KEKIYGPDAG---T----DYEDNQQRFSL	194
731	6492245	144	CYKRGVDRVFVDHPMFLE---KVWGKTGSKLYGPTT----G---T----DFRDNQLRFCL	189
682	MaizeSSIIb	316	FCKAAVEVPWYA-----PCGGTV-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	361
683	7489711	316	FCKAAVEVPWYA-----PCGGTV-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	361
684	15028467	312	FCKAAVEVPWFA-----PCGGSI-----YGDG----NLVFIANDWHTALLPVCLKAYYRD	357
685	15384987	255	FCKAAVEVPWFA-----PCGGSI-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	300
686	7489710	350	FCKVAVEVPWHV-----PCGGVC-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	395
687	16265834	428	FCKAAVEVPWHV-----PCGGVP-----YGDG----NLVFLANDWHTALLPVYLKAYYRD	473
688	15232051	410	FCKAAVEVPWYV-----PCGGVC-----YGDG----NLAFIANDWHTALLPVYLKAYYRD	455
689	7489695	109	FCKAAVEVPWHV-----PCGGVP-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	154
690	6467503	369	FCKAAVEVPWHV-----PCGGVC-----YGDG----NLAFIANDWHTALLPVYLKAYYRD	414
691	2833384	370	FCKAAVEVPWHV-----PCGGIC-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	415
692	2129898	370	FCKAAVEVPWHV-----PCGGIC-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	415
693	3192881	248	FCKAAIVVPWHV-----PCGGIC-----YGDG----NLVFIANDWHTALLPVYLKAYFRD	293
694	8953573	416	FCKAAVEVPWHV-----PCGGVP-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	461
695	7529653	417	FCKAAVEVPWHV-----PCGGVP-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	462
696	8953571	417	FCKAAVEVPWHV-----PCGGVP-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	462
697	5825480	417	FCKAAVEVPWHV-----PCGGVP-----YGDG----NLVFIANDWHTALLPVYLKAYYRD	462
698	14495348	367	LCKAAIEVPWYV-----PCGGYC-----YGDG----NLVFLANDWHTALLPVYLKAYYHD	412
699	2833390	405	FCKAAIEVPWHV-----PCGGVC-----YGDG----NLVFIANDWHTALLPAYLKAYYRD	450
700	8708896	194	LCKAALEAVWHV-----PCGGIT-----YGDD----NLCFIANDWHTALLPVYLQAHYRD	239
701	5880466	256	LCYAACEAPLIL-----ELGGYI-----YGQ-----NCMFVVNDWHASLVPVLLAAKYRP	300
702	9369334	256	LCYAACEAPLIL-----ELGGYI-----YGQ-----NCMFVVNDWHASLVPVLLAAKYRP	300
703	15237934	260	LCHAACEAPLVL-----PLGGFT-----YGEK----SL-FLVNDWHAGLVPILLAAKYRP	304
704	7188796	252	LCYAACEAPLIL-----ELGGYI-----YGQ-----SCMFVVNDWHASLVPVLLAAKYRP	296
705	6103327	256	LCYAACEAPLIL-----ELGGYI-----YGQ-----NCMFVVNDWHASLVPVLLAAKYRP	300
706	9369336	256	LCYAACEAPLIL-----ELGGYI-----YGQ-----NCMFVVNDWHASLVPVLLAAKYRP	300
707	6690399	183	LCHAACEAPLVL-----PLGGFT-----YGEK----SL-FLVNDWHAGLVPILLAAKYRP	227
708	2829792	249	LSHAACEAPLVL-----PLGGFT-----YGEK----CL-FLANDWHAALVPLLLAAKYRP	293
709	2833387	110	LCYAACEAPLIL-----ELGGYI-----YGQ-----NCMFVVNDWHASLVPVLLAAKYRP	154
710	12019656	256	LCYAACEAPLVL-----ELGGYI-----YGQ-----NCMFVVNDWHASLVPVLLAAKYRP	300
711	7489712	249	LCYAACEAPLIL-----ELGGYI-----YGQ-----NCMFVVNDWHASLVPVLLAAKYRP	293
712	5295947	250	LCYAACEAPLIL-----ELGGYI-----YGQ-----KCMFVVNDWHASLVPVLLAAKYRP	294
713	1549232	250	LCYAACEAPLIL-----ELGGYI-----YGQ-----KCMFVVNDWHASLVPVLLAAKYRP	294
714	2833377	250	LCYAACEAPLIL-----ELGGYI-----YGQ-----KCMFVVNDWHASLVPVLLAAKYRP	294
715	6136121	200	LCQAALEAPRVL-----NLTSSK-----YFSGPYGEDVVFVANDWHTALLPCYLKSMYQS	249
716	228210	199	LCQAALEAPKVL-----NLNSSN-----YFSGPYGEDVLFIANDWHTALIPCYLKSMYQS	248
717	297196	199	LCQAALEAPKVL-----NLNSSN-----YFSGPYGEDVLFIANDWHTALIPCYLKSMYQS	248
718	2833388	200	LCLAALEAPRVL-----NLNSSKNFSGPYGE-----EVAFIANDWHTALLPCYLKAIYQP	249
719	15637079	200	LCQAALEAPRVL-----NLNSSN-----YFSGPYGEDVVFVANDWHTALLPCYLKTMYQS	249
720	602594	199	LCQAALEAPKVL-----NLSSSN-----YFSGPYGEDVLFIANDWHTALIPCYLKSMYQS	248
721	2833381	200	LCQAALEAPRVL-----NLNSSK-----YFSGPYGEDVVFVANDWHTALLPCYLKTMYQS	249
722	5441242	198	LCQAALEAPRVL-----NLNSNK-----YFSGPYGEDVIFVANDWHTALLPCYLKSMYQT	247
723	15223331	202	LCQAALEAPQVL-----NLNSSK-----YFSGPYGEDVVFVANDWHTALLPCYLKSMYQS	251
724	12003285	196	LSLAALEAPRVL-----NLTSNK-----YFSGPYGEDVVFVANDWHTAVLPCYLKTIYQP	245
725	3832512	199	LCQAALEAPRVL-----NLNSNK-----HFSGPYGEDVVFVANDWHTALLPCYLKSLYKS	248
726	15626365	205	FCQAALEAARVL-----NLKSNK-----YFSGPYGEDVIFVANDWHTALISCYMKSMYQS	254
727	2833383	195	LCQAALEAPRVL-----NLNSSK-----YFSGPYGEDVIFVANDWHSALIPCYLKSMYKS	244
728	6624281	195	LCQAALEVPRILDLNNNPHFSGP-----YGE-----DVVFVCNDWHTGLLACYLKSNYQS	244
729	17736918	125	LCQAALEVPRIL-----NLDNNP-----YFSGPYGEDVVFVCNDWHTGLLACYLKSNYQS	174
730	4760584	195	LCQAALEVPRIL-----NLDNNP-----YFSGPYGEDVVFVCNDWHTGLLACYLKSNYQS	244
731	6492245	190	LCLAALEAPRVL-----NLNNSE-----YFSGPYGENVVFVANDWHTAVLPCYLKSMYKQ	239
682	MaizeSSIIb	362	NGLMQYARSVLVIHNIAHQGRGPVDDFVNFDLPEHYIDHF--------KLYDN----IG-	408
683	7489711	362	NGLMQYARSVLVIHNIAHQGRGPVDDFVNFDLPEHYIDHF--------KLYDN----IG-	408
684	15028467	358	NGLMQYTRSVLVIHNIAHQGRGPVDDFATMDLPEHYIDHF--------RLYDP----VG-	404
685	15384987	301	NGLMQYTRSVLVIHNIAHQGRGPVDDFATMDLPEHYIDHF--------RLYDP----VG-	347
686	7489710	396	HGLMQYTRSVLVIHNIAHQGRGPVDEFPYMDLPEHYLQRF--------ELYDP----VG-	442
687	16265834	474	NGMMQYTRSVLVIHNIAYQGRGPVDEFPYMELPEHYLDHF--------KLYDP----VG-	520
688	15232051	456	HGIMKYTRSVLVIHNIAHQGRGPVDDFSYVDLPSHYLDSF--------KLYDP----VG-	502
689	7489695	155	HGLMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYLEHF--------RLYDP----VG-	201
690	6467503	415	NGLMQYTRSVLVIHNIAHQGRGPSGDFSYVGLPEHYIDLF--------KLHDP----IG-	461
691	2833384	416	HGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLDLF--------KMYDP----VG-	462
692	2129898	416	HGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLDLF--------KMYDP----VG-	462
693	3192881	294	NGVMKFTRSVLVIHNIAHQGRGPMDDFSIVDLPAQYADLF--------KLYDP----VG-	340
694	8953573	462	HGLMQYTRSIMVIHNIAHQGRGFVDEFPFTELPEHYLEHF--------RLYDP----VG-	508
695	7529653	463	HGLMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYLEHF--------RLYDP----VG-	509
696	8953571	463	HGLMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYLEHF--------RLYDP----VG-	509
697	5825480	463	HGLMQYTRSIMVIHNIAHQGRGPVDEFPFTELPEHYLEHF--------RLYDP----VG-	509
698	14495348	413	NGFMIYARSVLVIHNIAHQGRGPLDDFSYLDLPVDYMDLF--------KLYDP----FG-	459
699	2833390	451	NGIMNYTRSVLVIHNIAHQGRGPLEDFSYVDLPPHYMDPF--------KLYDF----VG-	497
700	8708896	240	YGEMTYARCAFVIHNMAHQGRGPFVESEHLELNEEYRERF--------RLYDP----IG-	286
701	5880466	301	YGVYRDSRSTLVIHNLAHQGLEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	355
702	9369334	301	YGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPFEWYGALEWVFPEWARRHAL----DK-	355
703	15237934	305	YGVYKDARSILIIHNLAHQGVEPAATYTNLGLPSEW--------------YGA----VGW	346
704	7188796	297	YGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	351
705	6103327	301	YGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	355
706	9369336	301	YGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	355
707	6690399	228	YGVYKDARSILIIHNLAHQGVEPAATYTNLGLPSEW--------------YGA----VGW	269
708	2829792	294	YGVYKDARSIVAIHNIAHQGVEPAVTYNNLGLPPQWYGAVEWIFPTWARAHAL----DT-	348
709	2833387	155	YGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	209
710	12019656	301	YGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	355
711	7489712	294	YGVYKDSRSILVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	348
732	7484400	4	RGRGPFVESEHLELNEEYRERF--------RLYDP----IG-	32
712	5295947	295	YGVYRDARSVLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	349
713	1549232	295	YGVYRDARSVLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	349
714	2833377	295	YGVYRDARSVLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHAL----DK-	349
715	6136121	250	KGMYLHAKVAFCIHNIAYQGRFGSSDFCLLNLPDQFKSSF--------DFFDGYEKPVK-	300
716	228210	249	RGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRGSF--------DFIDGYEKPVK-	299
717	297196	249	RGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRGSF--------DFIDGYEKPVK-	299
718	2833388	250	MGIYKHAKVAFCIHNIAYQGRFAFSDFPRLNLPDKFKSSF--------DFIDGYEKPVK-	300
719	15637079	250	RGIYMNAKVAFCIHNIAYQGRFAFSDFSLLNLPDEYKGSF--------DFIDGYDKPVK-	300
720	602594	249	RGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRGSF--------DFIDGYEKPVK-	299
721	2833381	250	RGIYMNAKVAFCIHNIAYQGRFAFSDFSLLNLPDEYKGSF--------DFIDGYDKPVK-	300
722	5441242	248	RGVYRNTKVAFCIHNISYQGRHPFEDFPLLNLPNEYRSAF--------DFTDGHLKPVR-	298
723	15223331	252	RGVYMNAKVVFCIHNIAYQGRFAFDDYSLLNLPISFKSSF--------DFMDGYEKPVK-	302
724	12003285	246	KGIYTNAKVVLCIHNIAYQGRFAFSDFYKLNLPDQLKSSF--------DFMDGYEKPVK-	296
725	3832512	249	KGIYKSAKVAFCIHNIAYQGRHAFSDLSLLNLPNEFRSSF--------DFIDGYDKPVK-	299
726	15626365	255	IGIFRNAKVVFCIHNIAYQGRFAFTDYSLLNLPDQFKSSF--------DFLDGHVKPIV-	305
727	2833383	245	RGLYKNAKVAFCIHNIAYQGRNAFSDFSLLNLPDEFRSSF--------DFIDG----YNK	292
728	6624281	245	NGIYRTAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSF--------DFIDGYDKPVE-	295
729	17736918	175	NGIYRAAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSF--------DFIDGYDKPVE-	225
730	4760584	245	NGIYRAAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSF--------DFIDGYDKPVE-	295
731	6492245	240	NGIYVNAKVAFCIHNIAYQGRFPRVDFELLNLPESFMPSF--------DFVDGHVKPVV-	290

[0401]

TABLE XXVII
Maize soluble starch synthase IIb (SSIIb)
“LINKR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                Sequence                end	#
733	MaizeSSIIb	409	-------------GDHSNVFAAGLKTADRVVTVSNGYMWELKT-S-EG-GWGLHDIINQN	452
734	7489711@	409	-------------GDHSNVFAAGLKTADRVVTVSNGYMWELKT-S-EG-GWGLHDIINQN	452
735	15028467@	405	-------------GEHSNVFAAGLKMADRAVTVSHGYLWEIKT-M-DG-GWGLHEIINHN	448
736	15384987@	348	-------------GEHSNVFAAGLKMADRAVTVSHGYLWEIKT-M-DG-GWGLHEIINHN	391
737	7489710@	443	-------------GEHANIFAAGLKMADRVVTVSRGYLWELKT-V-EG-GWGLHDIIRSN	486
738	16265834@	521	-------------GEHANIFGAGLKMADRVVTVSPGYLWELKT-T-EG-GWGLHDIIREN	564
739	15232051@	503	-------------GEHFNIFAAGLKAADRVLTVSHGYSWEVKT-L-EG-GWGLHNIINEN	546
740	7489695@	202	-------------GEHANYFAAGLKMADQVVVVSPGYLWELKT-V-EG-GWGLHDIIRQN	245
741	6467503@	462	-------------GDHFNIFAPGLKVADRVVTVSHGYAWELKT-S-EG-GWGLHNIINEN	505
742	2833384@	463	-------------GEHFNIFAAGLKTADRIVTVSHGYAWELKT-S-EG-GWGLHNIINES	506
743	2129898@	463	-------------GEHFNIFAAGLKTADRIVTVSHGYAWELKT-S-EG-GWGLHNIINES	506
744	2192881@	341	-------------GDHFNIFAAGLKTADRVVTVSHGYAWELKT-S-EG-GWGLNGIRNEN	384
745	8953573@	509	-------------GEHANYFAAGLKMADQVVVVSPGYLWELKT-V-EG-GWGLHDIIRQN	552
746	7529653@	510	-------------GEHANYFAAGLKMADQVVVVSPGYLWELKT-V-EG-GWGLHDIIRQN	553
747	8953571@	510	-------------GEHANYFAAGLKMADQVVVVSPGYLWELKT-V-EG-GWGLHDIIRQN	553
748	5825480@	510	-------------GEHANYFAAGLKMADQVVVVSPGYLWELKT-V-EG-GWGLHDIIRQN	553
749	14495348@	460	-------------GDHLNIFAAGIKAADRLLTVSHGYAWELKT-A-EG-GWGLHGIINES	503
750	2833390@	498	-------------GEHFNIFAAGLKTADRVVTVSHGYSWELKT-S-QG-GWGLHQIINEN	541
751	8708896@	287	-------------GEHMNVMKAGLECAHRLVAVSKCYAWECQT-V-EG-GWGLHEVIKVN	330
752	5880466@	356	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	399
753	9369334@	356	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	399
754	15237934@	347	VFPTWARTHALDTGEAVNVLKGAIVTSDRIITVSQGYAWEITT-V-EG-GYGLQDLLSSR	403
755	7188796@	352	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	395
756	6103327@	356	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	399
757	9369336@	356	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	399
758	6690399@	270	VFPTWARTHALDTGEAVNVLKGAIVTSDRIITVSQGYAWEITT-V-EG-GYGLQDLLSSR	326
759	2829792@	349	-------------GETVNVLKGAIAVADRILTVSQGYSWEITT-P-EG-GYGLHELLSSR	392
760	2833387@	210	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	253
761	12019656@	356	-------------GEAVNFLKGAVVTADRIVTVSKGYSWEVTT-A-EG-GQGLNELLSSR	399
762	7489712@	349	-------------GEAVNFLKGAVVTADRIVTVSKGYSWEVTT-A-EG-GQGLNELLSSR	392
763	7484400@	33	-------------GEHMNVMKAGLECAHRLVAVSKCYAWECQT-V-EG-GWGLHEVIKVN	76
764	5295947@	350	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	393
765	1549232@	350	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	393
766	2833377@	350	-------------GEAVNFLKGAVVTADRIVTVSQGYSWEVTT-A-EG-GQGLNELLSSR	393
767	6136121@	301	-------------GRKINWMKAGILESDRVVTVSPYYAMELVSGA-EK-GVELDNVIAKT	345
768	228210@	300	-------------GRKINWMKAGILESHRVVTVSPYYAQELVS-AVDK-GVELDSVLRKT	344
769	297196@	300	-------------GRKINWMKAGILESHRVVTVSPYYAQELVS-AVDK-GVELDSVLRKT	344
770	2833388@	301	-------------GRKINWMKAGILESDRVLTVSPYYAQEVIS-GVER-GVELDNFIRKT	345
771	15637079@	301	-------------GRKINWMKAGIREADRVFTVSPNYAKELVS-CVSK-GVELDNHI--R	343
772	602594@	300	-------------GRKINWMKAGILESHRVVTVSPYYAQELVS-AVDK-GVELDSVLRKT	344
773	2833381@	301	-------------GRKINWMKAGIREADRVFTVSPNYAKELVS-CVSK-GVELDNHI--R	343
774	5441242@	299	-------------GRKINWMKAAILESDLVLTVSPYYARELVS-G-EDRGVELDNIIRKT	343
775	15223331@	303	-------------GRKINWMKAAILEAHRVLTVSPYYAQELIS-GVDR-GVELHKYLRMK	347
776	12003285@	297	-------------GRKINWMKAGIIESDRVLTVSPYYANELVS-GPDK-GVELDNILRK-	340
777	3832512@	300	-------------GRKINWMKAGVLESDRVFTVSPYYAKELVS-G-EDRGVELDNIIRS-	343
778	15626365@	306	-------------GRKINWMKAGIIESHRVLTVSPYYAQELVS-GPDK-GVELDNILRRV	350
779	2833383@	293	PCE----------GKKINWMKAGILESDQVFTVSPHYAKELIS-G-EDRGVELDNIIRST	340
780	6624281@	296	-------------GRKINWMKAGILQADKVLTVSPYYAEELIS-G-EARGCGLDNIMRLT	340
781	17736918@	226	-------------GRKINWMKAGILQADKVLTVSPYYAEELIS-G-ETRGCELDNIMRLT	270
782	4760584@	296	-------------GRKINWMKAGILQADKVLTVSPYYAEELIS-G-EARGCELDNIMRLT	340
783	6492245@	291	-------------GRKINWMKAGITECDVVLTVSPHYVKELTS-GPEK-GVELDGVLRAK	335
733	MaizeSSIIb	453	DWKLQGIVNGIDMSEWNPAVD----VHL----HSDDYTN--YTFETLDTGKRQCKAALQR	502
734	7489711@	453	DWKLQGIVNGIDMSEWNPAVD----VHL----HSDDYTN--YTFETLDTGKRQCKAALQR	502
735	15028467@	449	DWKLQGIVNGIDMAEWNPEVD----EHL----QSDGYAN--YTFETLDTGKKQCKEALQR	498
736	15384987@	392	DWKLQGIVNGIDMAEWNPEVD----EHL----QSDGYAN--YTFETLDTGKKQCKEALQR	441
737	7489710@	487	DWKINGIVNGIDHQEWNPKVD----VHL----RSDGYTN--YSLETLDAGKRQCKAALQR	536
738	16265834@	565	DWKMNGIVNGIDYREWNPEVD----VHL----QSDGYAN--YTVASLDSSKPRCKAALQR	614
739	15232051@	547	DWKFRGIVNGIDTQEWNPEFD----TYL----HSDDYTN--YSLENLHIGKPQCKAALQK	596
740	7489695@	246	DWKTRGIVNGIDNMEWNPEVD----AHL----KSDGYTN--FSLRTLDSGKRQCKEALQR	295
741	6467503@	506	HWKLQGIVNGIDAKEWNPQFD----IQL----TSDGYTN--YSLETLDTGKPQCKTALQN	555
742	2833384@	507	DWKFRGIVNGVDTKDWNPQFD----AYL----TSDGYTN--YNLKTLQTGKRQCKAALQR	556
743	2129898@	507	DWKFRGIVNGVDTKDWNPQFD----AYL----TSDGYTN--YNLKTLQTGKRQCKAALQR	556
744	3192881@	385	EWKLQGIVNGIDIEEWNPQLD----VYL----KSDGYAE--YSLDTLQTGKPQCKAALQK	434
745	8953573@	553	DWKTRGIVEGIDNMEWNPEVD----VHL----KSDGYTN--FSLGTLDSGKRQCKEALQR	602
746	7529653@	554	DWKTRGIVNGIDNMEWNPEVD----AHL----KSDGYTN--FSLRTLDSGKRQCKEALQR	603
747	8953571@	554	DWKTRGIVNGIDNMEWNPEVD----VHL----QSDGYTN--FSLSTLDSGKRQCKEALQR	603
748	5825480@	554	DWKTRGIVNGIDNMEWNPEVD----VHL----KSDGYTN--FSLGTLDSGKRQCKEALQR	603
749	14495348@	504	DWKFQGIVNGIDTTDWNPRCD----IHL----KSDGYTN--YSLETVQAGKQQCKAALQK	553
750	2833390@	542	DWKLQGIVNGIDTKEWNPELD----VHLP---RSDGYMN--YSLDTLQTGKPQCKAALQK	592
751	8708896@	331	NWKLRGIVNGIDYKEWNPICD----EFL----TTDGYAH--YDVDTLAEGKAKCKAALQK	380
752	5880466@	400	KSVLNGIVNGIDINDWNPTTD----KCL----PE----H--YSVDDL-SGKAKCKAELQK	444
753	9369334@	400	KSVLNGIVNGIDINDWNPTTD----KCL----PH----H--YSVDDL-SGKAKCKAELQK	444
754	15237934@	404	KSVINGITNGINVDEWNPSTD----EHIPFHYSADDVSE-----------KIKCKMALQK	448
755	7188796@	396	KSVLNGIVNGIDINDWNPTTD----KCL----PH----H--YSVDDL-SGKAKCKAELQR	440
756	6103327@	400	KSVLNGIVNGIDINDWNPTTD----KCL----PH----H--YSVDDL-SGKAKCKAELQK	444
757	9369336@	400	KSVLNGIVNGIDINDWNPTTD----KCL----PH----H--YSVDDL-SGKAKCKAELQK	444
758	6690399@	327	KSVINGITNGINVDEWNPSTD----EHIPFHYSADDVSE-----------KIKCKMALQK	371
759	2829792@	393	QSVLNGITNGIDVNDWNPSTD----EHI----AS----H--YSINDL-SGKVQCKTDLQK	437
760	2833387@	254	KSVLNGIVNGIDINDWNPTTD----KCL----PH----H--YSVDDL-SGKAKCKAELQK	298
761	12019656@	400	KSVLNGIVNGIDINDWNPATDKCIPCHY----SVDDL-----------SGKAKCKSALQK	444
762	7489712@	393	KSVLNGIVNGIDINDWNPATDKCIPCHY----SVDDL-----------SGKAKCKGALQK	437
763	7484400@	77	NWKLRGIVNGIDYKEWNPICD----EFL----TTDGYAH--YDVDTLAEGKAKCKAALQK	126
764	5295947@	394	KSVLNGIVNGIDINDWNPSTD----KFL----P----YH--YSVDDL-SGKAKCKAELQK	438
765	1549232@	394	KSVLNGIVNGIDINDWNPSTD----KFL----P----YH--YSVDDL-SGKAKCKAELQK	438
766	2833377@	394	KSVLNGIVNGIDINDWNPSTD----KFL----P----YH--YSVDDL-SGKAKCKAELQK	438
767	6136121@	346	--SITGIVNGMDTQEWNPATD----KHI----D----TN--YDITTVMDAKPLLKEALQA	389
768	228210@	345	--CITGIVNGMDTQEWNPA--------------TDKYTDVKYDITTVMDAKPLLKEALQA	388
769	297196@	345	--CITGIVNGMDTQEWNPA--------------TDKYTDVKYDITTVMDAKPLLKEALQA	388
770	2833388@	346	G--IAGIINGMDVQEWNPVTD----KYI----D----IH--YDATTVMDAKPLLKEALQA	389
771	15637079@	344	DCGITGICNGMDTQEWNPATD----KYL----A----VK--YDITTVEQAKPLLKEALQA	389
772	602594@	345	--CITGIVNGMDTQEWNPA--------------TDKYTDVKYDITTVMDAKPLLKEALQA	388
773	2833381@	344	DCGITGICNGMDTQEWNPATD----KYL----A----VK--YDITTVMQAKPLLKEALQA	389
774	5441242@	344	G--VAGIVNGMDIREWSPKTDKF--IDI----HFDT--------TSVKEAKFLLKEALQA	387
775	15223331@	348	--TVSGIINGMDVQEWNPSTD----KYI----D----IK--YDITTVTDAKPLIKEALQA	391
776	12003285@	341	-CTVTGIVNGMDTQEWNPATD----KYI----DN----H--YDITTVMDGKPLLKEALQA	385
777	3832512@	344	-IGITGIVNGMDNREWSPQTD----RYI----D----VH--YDASTVTEAKAILKEALQA	388
778	15626365@	351	G--VTGIVNGMDVQEWNPSTD----KYI----S----IK--YDASTVLEGKALLKEELQA	394
779	2833383@	341	G--IIGIVNGMDNREWSPQTD----RYI----D----VH--YNETTVTEAKPLLKGTLQA	384
780	6624281@	341	G--ITGIVNGMDVSEWDPIKD----KFL--------TVN--YDVTTALEGKALNKEALQA	384
781	17736918@	271	G--ITGIVNGMDVSEWDPTKD----KFL----A----VN--YDITTALEGKALNKEALQA	314
782	4760584@	341	G--ITGIVNGMDVSEWDPTKD----KFL----A----VN--YDITTALEGKALNKEALQA	384
783	6492245@	336	PLE-TGIVNGMDVVDWNPATD----KYI----S----VK--YNATTVAEARALNKEILQA	380

[0402]

TABLE XXVIII
Maize soluble starch synthase IIb (SSIIb)
“GLYTR” Domain Alignments with other similar Proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                 Sequence                 end	#
784	MaizeSSIIa	503	QLGLQVRDDVPLIGFIGRLDHQKGVDIIADAIHWI-AG-QDVQLVMLGTGRADLEDMLRR	560
785	7489711	503	QLGLQVRDDVPLIGFIGRLDHQKGVDIIADAIHWI-AG-QDVQLVMLGTGRADLEDMLRR	560
786	15028467	499	QLGLQVRDDVPLIGFIGRLDHQKGVDIIGDAMPWI-AG-QDVQVVMLGTGRPDLEEMLRR	556
787	15384987	442	QLGLQVRDDVPLIGFIGRLDHQKGVDIIGDAMPWI-AG-QDVQVVMLGTGRPDLEEMLRR	499
788	7489710	537	ELGLEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWI-AG-QDVQLVMLGTGRADLERMLQH	594
789	16265834	615	ELGLEVRDDVPLIGFIGRLDGQKGVDIIGDAMPWI-AG-QDVQLVLLGSGRRDLEVMLQR	672
790	15232051	597	ELGLPVRPDVPLIGFIGRLDHQKGVDLIAEAVPWM-MS-QDVQLVMLGTGRPDLEEVLRQ	654
791	7489695	296	ELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLQH	353
792	6467503	556	ELRFAIPPDVPVIGFIGRLDYQKGVDLIAEAIPWM-VG-QDVQLVMLGTGRQDLEEMLRQ	613
793	2833384	557	ELGLPVREDVPIISFIGRLDHQKGVDLIAEAIPWM-MS-HDVQLVMLGTGRADLEQMLKE	614
794	2129898	557	ELGLPVREDVPIISFIGRLDHQKGVDLIAEAIPWM-MS-HDVQLVMLGTGRADLEQMLKE	614
795	3192881	435	EMNLPVRDDVPLIGFIGRLDHQKGVDLIAEAIPWM-MG-QDVQLVMLGTGRPDLEQMLKQ	492
796	8953573	603	ELGLQVRGDVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLEGMLRH	660
797	7529653	604	ELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLQH	661
798	8953571	604	ELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLRH	661
799	5825480	604	ELGLQVRADVPLLGFIGRLDGQKGVEIIADAMPWI-VS-QDVQLVMLGTGRHDLESMLRH	661
800	14495348	554	ELGLPVRGDVPVIAFIGRLDHQKGVDLIAEAMPWI-AG-QDVQLIMLGTGRQDLEDTLRR	611
801	2833390	593	ELGLPVRDDVPLIGFIGRLDPQKGVDLIAEAVPWM-MG-QDVQLVMLGTGRRDLEQMLRQ	650
802	8708896	381	ELGLPVDPDAPMLGFIGRLDYQKGVDLIRDNYDYI-MG-EKCQLVMLGSGRQDLEDALRD	438
803	5880466	445	ELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMRS	502
804	9369334	445	ELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMRS	502
805	15237934	449	ELGLPIRPECPMIGFIGRLDYQKGIDLIQTAGPDL-MV-DDIQFVMLGSGDPKYESWMRS	506
806	7188796	441	ELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPDL-MR-EDVQFVMLGSGDPVFEGWMRS	498
807	6103327	445	ELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMRS	502
808	9369336	445	ELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMRS	502
809	6690399	372	ELGLPIRPECPMIGFIGRLDYQKGIDLIQTAGPDL-MV-DDIQFVMLGSGDPKYESWMRS	429
810	2829792	438	ELGLPIRPDCPLIGFIGRLDYQKGVDIILSAIPEL-MQ-NDVQVVMLGSGEKQYEDWMRH	495
811	2833387	299	ELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPEL-MR-EDVQFVMLGSGDPIFEGWMRS	356
812	12019656	445	ELGLPIRPEVPLIGFIGRLDYQKGIDLIQLIIPHL-MR-DDVQFVMLGSGDPELEDWMRS	502
813	7489712	438	ELGLPIRPDVFLIGFIGRLDYQKGIDLIQLIIPDL-MR-EDVQFVMLGSGDPELEDWMRS	495
814	7484400	127	ELGLPVDPDAPMLGFIGRLDYQKGVDLIRDNYDYI-MG-EKCQLVMLGSGRQDLEDALRD	184
815	5295947	439	ELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDL-MR-DNIQFVMLGSGDPGFEGWMRS	496
816	1549232	439	ELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDL-MR-DNIQFVMLGSGDPGFEGWMRS	496
817	2833377	439	ELGLPIRPDVPLIGFIGRLDYQKGIDLIKLAIPDL-MR-DNIQFVMLGSGDPGFEGWMRS	496
818	6136121	390	AVGLPVDKNIPVIGFIGRLEEQKGSDILVAAISKF-VG-LDVQIIILGTGKKKFEQQIQE	447
819	228210	389	AVGLPVDKKIPLIGFIGRLEEQKGSDILVAAIHKF-IG-LDVQIVVLGTGKKEFEQEIEQ	446
820	297196	389	AVGLPVDKKIPLIGFIGRLEEQKGSDILVAAIHKF-IG-LDVQIVVLGTGKKEFEQEIEQ	446
821	2833388	390	EVGLPVDRNVPLIGFIGRLEEQKGSDIFVAAISQL-VE-HNVQIVILGTGKKKFEKQIEH	447
822	15637079	390	AVGLPVDRNIPLIGFIGRLEEQKGSDILYAAISKF-IS-MDVQILILGTGKKKFEQQIEQ	447
823	602594	389	AVGLPVDKKVPLIGFIGRLEEQKGSDILVAAIHKF-IG-LDVQIVVLGTGKKEFEQEIEQ	446
824	2833381	390	AVGLPVDRNIPLIGFIGRLEEQKGSDILYAAISKF-IS-MDVQILILGTGKKKFEQQIEQ	447
825	5441242	388	EVGLPVNRDIPLIGFIGRLEEQKGSDILVEAIPKF-ID-QNVQIIILGTGKKSMEKQIEQ	445
826	15223331	392	AVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKF-MG-LNVQMVILGTGKKKMEAQILE	449
827	12003285	386	EVGLPVDRNVPLVGFIGRLEEQKGSDILVAALHKF-IE-MDVQVVILGTGKKEFEKQIEQ	443
828	3832512	389	EVGLPVDRNIPVIGFIGRLEEQKGSDILVESIPKF-ID-QNVQIIVLGTGKKIMEKQIEQ	446
829	15626365	395	EVGLPVDKNVPLIAFIGRLEEQKGSDILVEAIPQF-IK-ENVQIVALGTGKKEMEKQLQQ	452
830	2833383	385	EIGLPVDSSIPLIGFIGRLEEQKGSDILVEAIAKF-AD-ENVQIVVLGTGKKIMEKQIEV	442
831	6624281	385	EVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEI-VKEEDVQIVLLGTGKKKFERLLKS	443
832	17736918	315	EVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIVLLGTGKKKFERLLKS	373
833	4760584	385	EVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEILKE-EDVQIVLLGTGKKKFERLLKS	443
834	6492245	381	EVGLPVDSSIPVIVFIGRLEEQKGSDILIAAIPEF-LE-ENVQIIVLGTGKKKMEEELML	438
784	MaizeSSIIb	561	FESEHSDKVRAWVGFSVPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	620
785	7489711	561	FESEHSDKVRAWVGFSVPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	620
786	15028467	557	FESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYSTVPVVHAVGG	616
787	15384987	500	FESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	559
788	7489710	595	LEREHPNKVRGWVGFSVPMAHRITAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	654
789	16265834	673	FEAQHNSKVRGWVGFSVKMAHRITAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	732
790	15232051	655	MEHQYRDKARGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGG	714
791	7489695	354	FEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	413
792	6467503	614	FENQHRDKVRGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMMYGTIPVVHAVGG	673
793	2833384	615	FEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVGG	674
794	2129898	615	FEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVGG	674
795	3192881	493	IEGQYGDKVRGWVGFSVKTAHRITAGALILLMPSRFEPCGLNQLYAMSYGTVPVVHAVGG	552
796	8953573	661	FEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	720
797	7529653	662	FEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	721
798	8953571	662	FEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	721
799	5825480	662	FEREHHDKVRGWVGFSVRLAHRITAGADALLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	721
800	14495348	612	LESQHYDRVRGWVGFSIRLAHRMTAGADILLMPSRFEPCGLNQLYAMMYGTVPVVHAVGG	671
801	2833390	651	FECQHNDKIRGWVGFSVKTSHRITAGADILLMPSRFEPCALNQLYAMKYGTIPVVHAVGG	710
802	8708896	439	MENRNKNQCRGWVGFSNKMAHRITAAADILLMPSRFEPCGLNQLYAMAYGTVPIVHSVGG	498
803	5880466	503	TESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	562
804	9369334	503	TESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	562
805	15237934	507	MEETYRDKFRGWVGFNVPISHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPVVHGTGG	566
806	7188796	499	TESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	558
807	6103327	503	TESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	562
808	9369336	503	TESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	562
809	6690399	430	MEETYRDKFRGWVGFNVPISHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPVVHGTGG	489
810	2829792	496	TENLFKDKFRAWVGFNVPVSHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPIVHSTGG	555
811	2833387	357	TESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	416
812	12019656	503	TESDFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATGG	562
813	7489712	496	TESIFKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATGG	555
814	7484400	185	MENRNKNQCRGWVGFSNKMAHRITAAADILLMPSRFEPCGLNQLYAMAYGTVPIVHSVGG	244
815	5295947	497	TESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	556
816	1549232	497	TESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	556
817	2833377	497	TESGYRDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGG	556
818	6136121	448	LEVLYPDKARGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTIPICASTGG	507
819	228210	447	LEVLYPGKVKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTGG	506
820	297196	447	LEVLYPNKAKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTGG	506
821	2833388	448	LEVLYPDKARGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPIVASTGG	507
822	15637079	448	LEVMYPDKARGVAKFNVPLAHMITAGADFMLIPSRFEPCGLIQLHAMRYGTPCICASTGG	507
823	602594	447	LEVLYPNKAKGVAKFNVPLAHMITAGADFMLVPSRFEPCGLIQLHAMRYGTVPICASTGG	506
824	2833381	448	LEVMYPDKARGVAKFNVPLAHMITAGADFMLIPSRFEPCGLIQLHAMRYGTPCICASTGG	507
825	5441242	446	LEEIYPEKARGIAKFDGPLAHKIIAGSDFIMIPSRFEPCGLVQLHSMPYGTVPIVSSTGG	505
826	15223331	450	LEEKFPGKAVGVAKFNVPLATMITAGADFIIVPSRFEPCGLIQLHAMRYGTVPIVASTGG	509
827	12003285	444	LEELYPGKAVGVAKFNVPLAHKITAGADFMLVPSRFEPCGLIQLHAMRYGTIPICASTGG	503
828	3832512	447	LEVTYPGKAIGVAKFNSPLAHKIIAGADFIVIPSRFEPCGLVQLHAMPYGTVPIVSSTGG	506
829	15626365	453	LEISYPDKARGVAKFNVPLAHMMIAGADFILIPSRFEPCGLIQLQAMRYGTVPIVASTGG	512
830	2833383	443	LEEKYPGKAIGITKFNSPLAHKIIAGADFIVIPSRFEPCGLVQLHAMPYGTVPIVSSTGG	502
831	6624281	444	VEEKFPTKVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGG	503
832	17736918	374	IEEKFPSKVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGG	433
833	4760584	444	IEEKFPSKVRAVVRFNAPLAHQMMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGG	503
834	6492245	439	LEAKYPQNARGIAKFNVPLAHMMFAGANFIIVPSRFEPCGLIQLQGMRYGVIPICSSTGG	498
784	MaizeSSIIb	621	LRDTV---------AP-FDPF----NDT---GL----G---W--------TFDR---AEA	645
785	7489711	621	LRDTV---------AP-FDPF----NDT---GL----G---W--------TFDR---AEA	645
786	15028467	617	LRDTV---------AP-FDPF----ADT---GL----G---W--------TFDR---AEA	641
787	15384987	560	LRDTV---------AP-FDPF----ADT---GL----G---W--------TFDR---AEA	584
788	7489710	655	LRDTV---------AP-FDPF----GDA---GL----G---W--------TFDR---AEA	679
789	16265834	733	LRDTM---------SA-FDPF----EDT---GL----G---W--------TFDR---AEP	757
790	15232051	715	LRDTV---------QQ-FDPY----SET---GL----G---W--------TFDS---AEA	739
791	7489695	414	LRDTV---------PP-FDPF----NHS---GL----G---W--------TFDR---AEA	438
792	6467503	674	LRDTV---------QP-FDPF----NES---GL----G---W--------TFDS---AES	698
793	2833384	675	LRDTV---------QP-FNPF----DES---GV----G---W--------TFDR---AEA	699
794	2129898	675	LRDTV---------QP-FNPF----DES---GV----G---W--------TFDR---AEA	699
795	3192881	553	LRDTV---------QP-FDPF----NES---GY----G---W--------TFGR---AEA	577
796	8953573	721	LRDTV---------PP-FDPF----NHS---GL----G---W--------TFDR---AEA	745
797	7529653	722	LRDTV---------PP-FDPF----NHS---GL----G---W--------TFDR---AEA	746
798	8953571	722	LRDTV---------PP-FDPF----NHS---GL----G---W--------TFDR---AEA	746
799	5825480	722	VRDTV---------PP-FDPF----NHS---GL----G---W--------TFDR---AEA	746
800	14495348	672	LRDTV---------EH-YNPY----EES---GL----G---W--------TFEK---AEA	696
801	2833390	711	LRDTV---------QP-FDPL----MSQ---DW----G---G--------PSDR---AEA	735
802	8708896	499	LRDTV---------KQ-YSPF----ENV---GT----G---W--------VFER---AEA	523
803	5880466	563	LRDTV---------ET-FNPFGAK-GEE---GT----G---W--------AFSP---LTV	590
804	9369334	563	LRDTV---------ET-FNPFGAK-GEE---GT----G---W--------AFSF---LTV	590
805	15237934	567	LRDTV---------EN-FNPYAEGGAGT---GT----G---W--------VFTP---LSK	595
806	7188796	559	LRDTV---------ET-FNPFGAK-GEE---GT----G---W--------AFSP---LTV	586
807	6103327	563	LRDTV---------ET-FNPFGAK-GEE---GT----G---W--------AFSP---LTV	590
808	9369336	563	LRDTV---------ET-FNPFGAK-GEE---GT----G---W--------AFSP---LTV	590
809	6690399	490	LRDTV---------EN-FNPYAEGGAGA---GT----G---W--------VFTP---LSK	518
810	2829792	556	LRDTV---------KD-FNPY----AQE---GIGEGTG---W--------TFSP---LTS	584
811	2833387	417	LRDTV---------ET-FNPFGAK-GEE---GT----G---W--------AFSP---LTV	444
812	12019656	563	LRDTV---------EN-FNPF----GENGEQGT----G---W--------AFAP---LTT	590
813	7489712	556	LRDTV---------EN-FNPF----GENGEQGT----G---W--------AFAP---LTT	583
814	7484400	245	LRDTV---------KQ-YSPF----ENV---GT----G---W--------VFER---AEA	269
815	5295947	557	LRDTV---------EN-FNPFAEK-GEQ---GT----G---W--------AF	579
816	1549232	557	LRDTV---------EN-FNPFAEK-GEQ---GT----G---W--------AF	579
817	2833377	557	LRDTV---------EN-FNPFAEK-GEQ---GT----G---W--------AF	579
818	6136121	508	LVDTV---------TEGFTGF----HMG---AF----NVECA--------TVDP---ADV	536
819	228210	507	LVDTV---------K---EGY----TGF---HM----G---AFNVECD--VVDP---ADV	535
820	297196	507	LVDTV---------K---EGY----TGF---HM----G---AFNVECD--VVDP---ADV	535
821	2833388	508	LVDTV---------KEGYTGF----QMG---AL----H---V--------ECDKIDSADV	536
822	15637079	508	LVDTV---------K---EGY----TGF---HM----G---AFNVDCE--TVDP---EDV	536
823	602594	507	LVDTV---------K---EGY----TGF---HM----G---AFNVECD--VVDP---ADV	535
824	2833381	508	LVDTV---------K---EGY----TGF---HM----G---AFNVDCE--TVDP---EDV	536
825	5441242	506	LVDTV---------Q---EGF----TGF---HM----G---AFNVDCE--AIDP---ADV	534
826	15223331	510	LVDTV---------K---DGY----TGF---HI----G---RFNVKCE--VVDP---DDV	538
827	12003285	504	LVDTV---------K---EGF----TGF---HM----G---AFNVECD--AVDP---ADV	532
828	3832512	507	LVDTV---------KEGYTGF----HVG---AF----SVECE--------AVDP---ADV	535
829	15626365	513	LVDTV---------K---EGF----TGF---HM----G---SFNVKCD--AVDP---VDV	541
830	2833383	503	LVDTVKEGYTGFHAGP-FDVE----CE-----------------------DVDP---DDV	531
831	6824281	504	LVDTI---------VE---------GKT---GF----H---MGRLSVDCNVVEP---ADV	532
832	17736918	434	LVDTI---------VE---------GKT---GF----H---MGRLSVDCNVVEP---ADV	462
833	4760584	504	LVDTI---------VE---------GKT---GF----H---MGRLSVDCNVVEP---ADV	532
834	6492245	499	LVDTV---------SEGVTGF----HMG---SF----N---V--------EFET---VDP	524

[0403]

TABLE XXIX
Maize soluble starch synthase IIb (SSIIb)
“CTEND” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                    Sequence                    end	#
835	MaizeSSIIb	646	NRMIDALSHCLTTY--RN--YKE-SWRACRARGMAEDLSWDHAAVLYEDVLVKAKYQW	698
836	7489711	646	NRMIDALSHCLTTY--RN--YKE-SWRACRARGMAEDLSNDHAAVLYEDVLVKAKYQW	698
837	15028467	642	NRMIDALGHCLNTY--RN--YKE-SWRGLQARGMAQDLSWDRAAELYEDVLVKAKYQW	694
838	15384987	585	NRMIDALGHCLNTY--RN--YKE-SWRGLQARGMAQDLSWDHAAELYEDVLVKAKYQW	637
839	7489710	680	NKLIEALRHCLDTY--RK--YGE-SWKSLQARGMSQDLSWDHAAELYEDVLVKAKYQW	732
840	16265834	758	HKLIEALGHCLETY--RK--YKE-SWRGLQVRGMSQDLSWDHAAELYEEVLVKAKYQW	810
841	15232051	740	GKLIHALGNCLLTY--RE--YKE-SWEGLQRRGMTQDLSWDNAAEKYEEVLVAAKYHW	792
842	7489695	439	HKLIEALGHCLRTY--RD--FKE-SWRALQERGMSQDFSWEHAAKLYEDVLVKAKYQW	491
843	6467503	699	HKLIHALGNCLLTY--RE--YKK-SWEGLQRRGMTPNLSWDHAAEKYEETLVAAKYQW	751
844	2833384	700	NKLMAALWNCLLTY--KD--YKK-SWEGIQERGMSQDLSWDNAAQQYEEVLVAAKYQW	752
845	2129898	700	NKLMAALWNCLLTY--KD--YKK-SWEGIQERGMSQDLSWDNAAQQYEEVLVAAKYQW	752
846	3192881	578	NQLIDALGNCLLTY--RQ--YKQ-SWEGLQRRGMMQDLSWDHAAEKYEEVLVAAKYQW	630
847	8953573	746	QKLIEALGHCLRTY--RD--YKE-SWRGLQERGMSQDFSWEHAAKLYEDVLVKAKYQW	798
848	7529653	747	HKLIEALGHCLRTY--RD--FKE-SWRALQERGMSQDFSWEHAAKLYEDVLVKAKYQW	799
849	8953571	747	HKLIEALGHCLRTY--RD--YKE-SWRGLQERGMSQDFSWEHAAKLYEDVLLKAKYQW	799
850	5825480	747	HKLIEALGHCLRTY--RD--YKE-SWRGLQERGMSQDFSWEHAAKLYEDVLLKAKYQW	799
851	14495348	697	NRLIDALGHCLNTY--RN--YRT-SWEGLQKRGMMQDLSWDNAAKLYEEVLLAAKYQW	749
852	2833390	736	SQLIPRIRNCLLTY--RE--YKK-SWEGIQTRCMTQDLSWDNAAQNYEEVLIAAKYQW	788
853	8708896	524	NKLRESINNALYTY--RQ--FRD-SFRGIQRRGMEQDLTWDNAASIYEEVLVAAKYQW	576
854	5880466	591	DKMLWALRTAMSTF--RE--HKP-SWEGLMKRGMTKDHTWDHAAEQYEQI	635
855	9369334	591	DKMLWALRTAMSTF--RE--HKP-SWEGLMKRGMTKDHTWDHAAEQYEQI	635
856	15237934	596	DSMVSALRLAAATY--RE--YKQ-SWEGLMRRGMTRNYSWENAAVQYEQV-----FQW	643
857	7188796	587	EKMLWALRTAISTF--RE--HKP-SWEGLMKRGMTKDHTWDHAAEQYEQI	631
858	6103327	591	DKMLWALRTAMSTF--RE--HKP-SWEGLMKRGMTKDHTWDHAAEQYEQI	635
859	9369336	591	DKMLWALRTANSTF--RE--HKP-SWEGLMKRGMTKDHTWDRAAEQYEQI	635
860	6690399	519	DSMVSALRLAAATY--RE--YKQ-SWEGLMRRGMTRNYSWENAAVQYEQV-----FQW	566
861	2829792	585	EKLLDTLKLAIGTY--TE--HKS-SWEGLMRRGMGRDYSWENAAIQYEQVFTWA	633
862	2833387	445	DKMLWALRTAMSTF--RE--HKP-SWEGLMKRGMTKDHTWDHA	482
863	12019656	591	ENMFVDIANC	600
864	7489712	584	ENMFVDIANC	593
865	7484400	270	NKLRESINNALYTY--RQ--FRD-SFRGIQRRGMEQDLTWDNAASIYEEVLVAAKYQW	322
866	6136121	537	QKIATTVERALAAY--GSVAYKE-MIQNC----MAQDLSWKGPAKNWEKMLL	581
867	228210	536	LKIVTTVAPALAVY--GTLAFAE-MIKNC----MSEELSWKEPAKKWETLLL	580
868	267196	536	LKIVTTVARALAVY--GTLAFAE-MIKNC----MSEELSWKEPAKKWETLLL	580
869	2833388	537	AAIVKTVAPALGTY--AT----A-ALREMILNCMAQDLSWKGPARMWEKMLL	581
870	15637079	537	LKVITTVGRALAMY--GTLAFTE-MIKNC----MSQELSWKGPAKNWETVLL	581
871	602594	536	LKIVTTVARALAVY--GTLAFAE-MIKNC----MSEELSWKEPAKKWETLLL	580
872	2833381	537	LKVITTVGRALAIY--GTLAFTE-MIKNC----MSQELSWKGPAKNWETVLL	581
873	5441242	535	EKIATTVRRALGTY--GT--V---AMEKIIQNCMAQDFSWKGPAKQWEKVL	578
874	15223331	539	IATAKPNTRAVAVYGTSA--MQE-MVKNC----MDQDFSWKGPARLWEKVLL	583
875	12003285	533	LKIVKTVGRALEVY--GTPAFRE-MINNC----MSLDLSWKGPAKNWETVLL	577
876	3832512	536	EKLATTVNRALKTYGTQA--LKE-MILNC----MAQDFSWKGPAKQWEQALL	580
877	15626365	542	DAIPKTVTKALGVYGTSA--FAE-MIKNC----MAQELSWKGPAKKWEEVLL	586
878	2833383	532	DKLAATVKRALKTY--GT----Q-AMKQIILNCMAQNFSWKKPAKLWEKALL	576
879	6624281	533	KKVVTTLKRAVKVV--GTPAYHE-MVKNC----MIQDLSWKGPAKNWEDVLLE	578
880	17736918	463	KKVVTTLKRAVKVV--GTPAYHE-MVKNC----MIQDLSWKGPAKNWEDVLLE	508
881	4760584	533	KKVVTTLKRAVKVV--GTPAYHE-MVKNC----MIQDLSWKGPAKNWEDVLLE	578
882	6492245	525	ADVAAVASNVTRAL--KQ--YKTPSFHAMVQNCMAQDLSWKGPAKKWEEALL	572

[0404]

TABLE XXX
Identities of the Accession Numbers used in Tables. XXV-XXIX.
Accession	Brief Description of sequences	score
Id.	producing significant alignments	(bits)	E−value
gi|7489711|pir||T01209	ADPglucose - starch glucosyltransfera . . .	1240	0.0 h
gi|15028467|gb|AAK81729.1|AF395537_1	(AF395537) soluble sta . . .	972	0.0
gi|15384987|emb|CAC59826.1|	(AJ308110) soluble starch synth . . .	968	0.0
gi|7489710|pir||T01208	ADPglucose - starch glucosyltransfera . . .	869	0.0
gi|16265834|gb|AAL16661.1|AF419099_1	(AF419099) putative so . . .	847	0.0
gi|15232051|ref|NP_186767.1|	(NM_110984) putative glycogen . . .	837	0.0
gi|7489695|pir||T06798	probable starch synthase (EC 2.4.1.— . . .	833	0.0
gi|6467503|gb|AAF13168.1|AF173900_1	(AF173900) granule boun . . .	832	0.0
gi|2833384|sp|Q43093|UGS3_PEA	Glycogen [starch] synthase, c . . .	832	0.0
gi|2129898|pir||S61505	UDPglucose - starch glucosyltransfera . . .	831	0.0
gi|3192881|gb|AAC19119.1|	(AF068834) starch synthase [Ipomo . . .	823	0.0
gi|8953573|emb|CAB96627.1|	(AJ269504) starch synthase IIa-3 . . .	814	0.0
gi|7529653|emb|CAB86618.1|	(AJ269502) starch synthase IIa-1 . . .	812	0.0
gi|8953571|emb|CAB96626.1|	(AJ269503) starch synthase IIa-2 . . .	811	0.0
gi|5825480|gb|AAD53263.1|AF155217_1	(AF155217) starch synth . . .	809	0.0
gi|14495348|gb|AAK64284.1|AF383878_1	(AF383878) soluble sta . . .	802	0.0
gi|2833390|sp|Q43847|UGS3_SOLTU	Glycogen [starch] synthase, . . .	785	0.0
gi|8708896|gb|AAC17970.2|	(AF026421) soluble starch synthas . . .	669	0.0
gi|5880466|gb|AAD54661.1|	(AF091803) starch synthase I [Tri . . .	456	e−127
gi|9369334|emb|CAB99209.1|	(AJ292521) starch synthase I-1 [. . .	456	e−127
gi|15237934|ref|NP_197818.1|	(NM_122336) soluble starch syn . . .	455	e−127
gi|7188796|gb|AAF37876.1|AF234163_1	(AF234163) starch synth . . .	454	e−126
gi|6103327|gb|AAF03557.1|	(AF091802) starch synthase I [Aeg . . .	454	e−126
gi|9369336|emb|CAB99210.1|	(AJ292522) starch synthase I-2 [. . .	454	e−126
gi|6690399|gb|AAF24126.1|AF121673_1	(AF121673) soluble star . . .	453	e−126
gi|2829792|sp|P93568|UGS2_SOLTU	Soluble glycogen [starch] s . . .	449	e−125
gi|2833387|sp|Q43654|UGS2_WHEAT	Soluble glycogen [starch] s . . .	448	e−125
gi|12019656|gb|AAD45815.2|	(AF168786) soluble starch syntha . . .	423	e−117
gi|7489712|pir||T01414	ADPglucose - starch glucosyltransfera . . .	422	e−117
gi|7484400|pir||T07924	probable starch synthase (EC 2.4.1.— . . .	413	e−114
gi|5295947|dbj|BAA81848.1|	(AB026295) ESTs AU075322(C11109) . . .	412	e−114
gi|1549232|dbj|BAA07396.1|	(D38221) SSS1 [Oryza sativa] >gi . . .	412	e−114
gi|2833377|sp|Q40739|UGS2_QRYSA	Soluble glycogen [starch] s . . .	411	e−113
gi|6136121|sp|O82627|UGST_ANTMA	Granule-bound glycogen [sta . . .	346	4e−94
gi|228210|prf||1718316A	granule-bound starch synthase [Sola . . .	327	2e−88
gi|267196|sp|Q00775|UGST_SOLTU	Granule-bound glycogen [star . . .	327	3e−88
gi|2833388|sp|Q43784|UGST_MANES	Granule-bound glycogen [sta . . .	327	3e−88
gi|15637079|dbj|BAB68126.1|	(AB071604) granule-bound starch . . .	326	5e−88
gi|602594|emb|CAA58220.1|	(X83220) starch (bacterial glycog . . .	326	6e−88
gi|2833381|sp|Q42857|UGST_IPOBA	Granule-bound glycogen [sta . . .	320	2e−86
gi|5441242|dbj|BAA82346.1|	(AB029546) granule-bound starch . . .	318	9e−86
gi|15223331|ref|NP_174566.1|	(NM_103023) starch synthase, p . . .	318	1e−85
gi|12003285|gb|AAG43519.1|AF210699_1	(AF210699) granule-bou . . .	316	6e−85
gi|3832512|gb|AAC70779.1|	(AF097922) granule-bound glycogen . . .	314	2e−84
gi|15626365|emb|CAC69955.1|	(AJ345045) granule-bound starch . . .	313	3e−84
gi|2833383|sp|Q43092|UGST_PEA	Granule-bound glycogen [starc . . .	312	1e−83
gi|6624281|dbj|BAA88509.1|	(AB029061) starch synthase (GBSS . . .	311	1e−83
gi|17736918|gb|AAL41028.1|	(AF250137) mutant granule bound . . .	311	2e−83
gi|4760584|dbj|BAA77352.1|	(AB019624) starch synthase (GBSS . . .	311	2e−83
gi|6492245|gb|AAF14233.1|AF109395_1	(AF109395) granule-boun . . .	309	5e−83
gi|6624285|dbj|BAA88511.1|	(AB029063) starch synthase (GBSS . . .	308	1e−82
gi|11037536|gb|AAG27624.1|AF286320_1	(AF286320) granule bou . . .	308	1e−82
gi|4760582|dbj|BAA77351.1|	(AB019623) starch synthase (GBSS . . .	308	1e−82
gi|6318540|gb|AAF06937.1|AF110374_1	(AF110374) granule-boun . . .	308	1e−82
gi|6318538|gb|AAF06936.1|AF110373_1	(AF110373) granule-boun . . .	307	2e−82
gi|6624287|dbj|BAA88512.1|	(AB029064) starch synthase (GBSS . . .	306	3e−82
gi|18652407|gb|AAL77109.1|AF474373_6	(AF474373) granule-bou . . .	306	3e−82
gi|136755|sp|P09842|UGST_HORVU	Granule-bound glycogen [star . . .	306	4e−82
gi|18139611|gb|AAL58572.1|	(AY069940) granule binding starc . . .	305	8e−82
gi|4760580|dbj|BAA77350.1|	(AB019622) starch synthase (GBSS . . .	305	1e−81
gi|4588609|gb|AAD26156.1|AF113844_1	(AF113844) granule-boun . . .	305	2e−81
gi|136765|sp|P27736|UGST_WHEAT	Granule-bound glycogen [star . . .	304	2e−81
gi|6624283|dbj|BAA88510.1|	(AB029062) starch synthase (GBSS . . .	303	3e−81
gi|16716335|gb|AAC17969.3|	(AF026420) granule-bound starch . . .	300	3e−80
gi|82478|pir||JQ0703	UDPglucose - starch glucosyltransferase . . .	299	6e−80
gi|2833385|sp|Q43134|UGST_SORBI	Granule-bound glycogen [sta . . .	298	1e−79
gi|15900991|ref|NP_345595.1|	(NC_003028) glycogen synthase . . .	298	1e−79
gi|15903076|ref|NP_358626.1|	(NC_003098) Glycogen synthase . . .	298	2e−79
gi|2833382|sp|Q42968|UGST_ORYGL	Granule-bound glycogen [sta . . .	297	2e−79
gi|297424|emb|CAA46294.1|	(X65183) glycogen (starch) syntha . . .	296	3e−79
gi|136758|sp|P19395|UGST_ORYSA	Granule-bound glycogen [star . . .	296	5e−79
gi|7798551|gb|AAC61675.2|	(AF031162) granule-bound starch s . . .	296	5e−79
gi|297422|emb|CAA45472.1|	(X64108) starch granule-bound sta . . .	292	1e−77
gi|4588607|gb|AAD26155.1|AF113843_1	(AF113843) granule-boun . . .	289	9e−77
gi|136757|sp|P04713|UGST_MAIZE	Granule-bound glycogen [star . . .	288	1e−76
gi|15643657|ref|NP_228703.1|	(NC_000853) glycogen synthase . . .	285	9e−76
gi|16080147|ref|NP_390973.1|	(NC_000964) starch (bacterial . . .	276	7e−73
gi|15672681|ref|NP_266855.1|	(NC_002662) glycogen synthase . . .	273	6e−72
gi|17366711|sp|Q9CHM9|GLGA_LACLA	Glycogen synthase (Starch . . .	273	6e−72
gi|2811062|sp|O08328|GLGA_BACST	Glycogen synthase (Starch [. . .	267	2e−70
gi|18309046|ref|NP_560980.1|	(NC_003366) glycogen synthase . . .	264	2e−69
gi|15613648|ref|NP_241951.1|	(NC_002570) starch (bacterial . . .	258	1e−67
gi|15895507|ref|NP_348856.1|	(NC_003030) Glycogen synthase, . . .	255	1e−66
gi|17229371|ref|NP_485919.1|	(NC_003272) glycogen synthase . . .	246	6e−64
gi|15641730|ref|NP_231362.1|	(NC_002505) glycogen synthase . . .	236	6e−61
gi|15620537|gb|AAL03921.1|U30252_9	(U30252) GlgA [Synechoco . . .	235	1e−60
gi|16331219|ref|NP_441947.1|	(NC_000911) glycogen synthase . . .	235	1e−60
gi|16766821|ref|NP_462436.1|	(NC_003197) glycogen synthase . . .	229	7e−59
gi|16762766|ref|NP_458383.1|	(NC_003198) glycogen synthase . . .	228	2e−58
gi|4582783|emb|CAB40375.1|	(AJ006752) starch synthase, isof . . .	226	4e−58
gi|17938870|ref|NP_535658.1|	(NC_003306) glycogen synthase . . .	225	2e−57
gi|16119514|ref|NP_396220.1|	(NC_003064) AGR_pAT_410p [Agro . . .	224	2e−57
gi|16124067|ref|NP_407380.1|	(NC_003143) glycogen synthase . . .	223	6e−57
gi|15717885|gb|AAK97773.1|	(AY044844) starch synthase isofo . . .	222	1e−56
gi|15803938|ref|NP_289974.1|	(NC_002655) glycogen synthase . . .	220	3e−56
gi|15890897|ref|NP_356569.1|	(NC_003063) AGR_L_1562p [Agrob . . .	219	9e−56
gi|15236819|ref|NP_193558.1|	(NM_117934) starch synthase-li . . .	216	8e−55
gi|17227527|ref|NP_484075.1|	(NC_003272) glycogen (starch) . . .	215	1e−54
gi|13476305|ref|NP_107875.1|	(NC_002678) glycogen synthase . . .	214	3e−54
gi|6116748|dbj|BAA85761.1|	(AB028026) granule-bound starch . . .	213	6e−54
gi|16329217|ref|NP_439945.1|	(NC_000911) glycogen (starch) . . .	212	1e−53
gi|9587321|gb|AAF89262.1|AF285987_1	(AF285987) granule-boun . . .	211	2e−53
gi|15602409|ref|NP_245481.1|	(NC_002663) GlgA [Pasteurella . . .	211	2e−53
gi|9587293|gb|AAF89248.1|AF285973_1	(AF285973) granule-boun . . .	211	2e−53
gi|14279432|gb|AAK58596.1|AF268969_2	(AF268969) glycogen sy . . .	211	3e−53
gi|7489826|pir||T01265	starch synthase DULL1 - maize >gi|30 . . .	210	5e−53
gi|17366749|sp|Q9EUT5|GLGA_RHITR	Glycogen synthase (Starch . . .	209	6e−53
gi|4582789|emb|CAB40374.1|	(AJ225088) Starch synthase isofo . . .	209	1e−52
gi|15966599|ref|NP_386952.1|	(NC_003047) PROBABLE GLYCOGEN . . .	208	2e−52
gi|9587317|gb|AAF89260.1|AF285985_1	(AF285985) granule-boun . . .	207	2e−52
gi|15606118|ref|NP_213495.1|	(NC_000918) glycogen synthase . . .	207	4e−52
gi|9587301|gb|AAF89252.1|AF285977_1	(AF285977) granule-boun . . .	206	5e−52
gi|146139|gb|AAA23870.1|	(J02616) glycogen synthase (EC 2.4 . . .	206	7e−52
gi|15221083|ref|NP_172637.1|	(NM_101044) putative glycogen . . .	205	2e−51
gi|18461221|dbj|BAB84418.1|	(AP003292) putative starch synt . . .	204	2e−51
gi|9587329|gb|AAF89266.1|AF285991_1	(AF285991) granule-boun . . .	203	6e−51
gi|16265159|ref|NP_437951.1|	(NC_003078) putative glycogen . . .	202	9e−51
gi|9587352|gb|AAF89276.1|AF286003_1	(AF286003) granule-boun . . .	202	1e−50
gi|7489274|pir||T07663	soluble starch synthase (EC 2.4.1.—) . . .	201	3e−50
gi|9587309|gb|AAF89256.1|AF285981_1	(AF285981) granule-boun . . .	200	4e−50
gi|17548463|ref|NP_521803.1|	(NC_003296) PROBABLE GLYCOGEN . . .	200	5e−50
gi|9587343|gb|AAF89273.1|AF285998_1	(AF285998) granule-boun . . .	200	5e−50
gi|9587311|gb|AAF89257.1|AF285982_1	(AF285982) granule-boun . . .	199	6e−50
gi|2833389|sp|Q43846|UGS4_SOLTU	Soluble glycogen [starch] s . . .	199	1e−49
gi|17646328|gb|AAL40942.1|AF432915_1	(AF432915) putative st . . .	198	1e−49
gi|9587337|gb|AAF89270.1|AF285995_1	(AF285995) granule-boun . . .	198	2e−49
gi|9587341|gb|AAF89272.1|AF285997_1	(AF285997) granule-boun . . .	197	3e−49
gi|9587305|gb|AAF89254.1|AF285979_1	(AF285979) granule-boun . . .	197	3e−49
gi|16273270|ref|NP_439511.1|	(NC_000907) glycogen synthase . . .	197	4e−49
gi|9587348|gb|AAF89274.1|	(AF286001) granule-bound starch s . . .	197	4e−49
gi|9587319|gb|AAF89261.1|AF285986_1	(AF285986) granule-boun . . .	196	6e−49
gi|8901183|gb|AAC17971.2|	(AF026422) soluble starch synthas . . .	195	1e−48
gi|9587307|gb|AAF89255.1|AF285980_1	(AF285980) granule-boun . . .	194	2e−48
gi|9587323|gb|AAF89263.1|AF285988_1	(AF285988) granule-boun . . .	194	2e−48
gi|9587313|gb|AAF89258.1|AF285983_1	(AF285983) granule-boun . . .	193	6e−48
gi|9587299|gb|AAF89251.1|AF285976_1	(AF285976) granule-boun . . .	191	2e−47
gi|9587327|gb|AAF89265.1|AF285990_1	(AF285990) granule-boun . . .	191	2e−47
gi|9587325|gb|AAF89264.1|AF285989_1	(AF285989) granule-boun . . .	190	4e−47
gi|9587297|gb|AAF89250.1|AF285975_1	(AF285975) granule-boun . . .	190	5e−47
gi|17367070|sp|Q9RNH6|GLGA_RHOSH	Glycogen synthase (Starch . . .	190	5e−47
gi|5834407|gb|AAD53959.1|AF181035_3	(AF181035) glycogen syn . . .	190	5e−47
gi|9587295|gb|AAF89249.1|AF285974_1	(AF285974) granule-boun . . .	188	2e−46
gi|9587333|gb|AAF89268.1|AF285993_1	(AF285993) granule-boun . . .	187	3e−46
gi|9502145|gb|AAF88000.1|	(AF258609) starch synthase III [A . . .	187	4e−46
gi|15597361|ref|NP_250855.1|	(NC_002516) probable glycogen . . .	186	6e−46
gi|9587339|gb|AAF89271.1|AF285996_1	(AF285996) granule-boun . . .	186	7e−46
gi|9502143|gb|AAF87999.1|AF258608_1	(AF258608) starch synth . . .	186	7e−46
gi|9587303|gb|AAF89253.1|AF285978_1	(AF285978) granule-boun . . .	186	7e−46
gi|9587335|gb|AAF89269.1|AF285994_1	(AF285994) granule-boun . . .	185	2e−45
gi|9587331|gb|AAF89267.1|AF285992_1	(AF285992) granule-boun . . .	182	9e−45
gi|17366350|sp|P58395|GLGA_THECA	Glycogen synthase (Starch . . .	180	4e−44
gi|15805621|ref|NP_294317.1|	(NC_001263) glycogen synthase . . .	180	5e−44
gi|15983795|gb|AAL10494.1|	(AY056803) Atlg32900/F9L11_8 [Ar . . .	179	9e−44
gi|17367076|sp|Q9RWS1|GLGA_DEIRA	Glycogen synthase (Starch . . .	177	5e−43
gi|15605532|ref|NP_220318.1|	(NC_000117) Glycogen Synthase . . .	170	6e−41
gi|3493107|gb|AAD03011.1|	(AF079291) granule-bound starch s . . .	167	4e−40
gi|3493007|gb|AAD02961.1|	(AF079241) granule-bound starch s . . .	166	6e−40
gi|3493103|gb|AAD03009.1|	(AF079289) granule-bound starch s . . .	166	6e−40
gi|7484399|pir|T07926	probable starch synthase (EC 2.4.1.— . . .	165	1e−39
gi|12278417|gb|AAG48949.1|	(AY010962) granule-bound starch . . .	164	2e−39
gi|3493005|gb|AAD02960.1|	(AF079240) granule-bound starch s . . .	164	2e−39
gi|3493111|gb|AAD03013.1|	(AF079293) granule-bound starch s . . .	164	3e−39
gi|15834801|ref|NP_296560.1|	(NC_002620) glycogen synthase . . .	164	3e−39
gi|12278503|gb|AAG48992.1|	(AY011005) granule-bound starch . . .	164	3e−39
gi|12278507|gb|AAG48994.1|	(AY011007) granule-bound starch . . .	164	4e−39
gi|3493117|gb|AAD03016.1|	(AF079296) granule-bound starch s . . .	163	5e−39
gi|3493079|gb|AAD02997.1|	(AF079277) granule-bound starch s . . .	163	5e−39
gi|12278509|gb|AAG48995.1|	(AY011008) granule-bound starch . . .	163	6e−39
gi|3493089|gb|AAD03002.1|	(AF079282) granule-bound starch s . . .	163	6e−39
gi|3493065|gb|AAD02990.1|	(AF079270) granule-bound starch s . . .	163	6e−39
gi|3493059|gb|AAD02987.1|	(AF079267) granule-bound starch s . . .	163	7e−39
gi|3493093|gb|AAD03004.1|	(AF079284) granule-bound starch s . . .	162	9e−39
gi|12278497|gb|AAG48989.1|	(AY011002) granule-bound starch . . .	162	9e−39
gi|3493113|gb|AAD03014.1|	(AF079294) granule-bound starch s . . .	162	9e−39
gi|12278505|gb|AAG48993.1|	(AY011006) granule-bound starch . . .	162	9e−39
gi|3493101|gb|AAD03008.1|	(AF079288) granule-bound starch s . . .	162	1e−38
gi|12278487|gb|AAG48984.1|	(AY010997) granule-bound starch . . .	162	1e−38
gi|3493061|gb|AAD02988.1|	(AF079268) granule-bound starch s . . .	162	1e−38
gi|3493049|gb|AAD02982.1|	(AF079262) granule-bound starch s . . .	162	1e−38
gi|3493019|gb|AAD02967.1|	(AF079247) granule-bound starch s . . .	162	1e−38
gi|12278514|gb|AAG48997.1|	(AY011011) granule-bound starch . . .	162	1e−38
gi|12278473|gb|AAG48977.1|	(AY010990) granule-bound starch . . .	162	2e−38
gi|3493121|gb|AAD03018.1|	(AF079298) granule-bound starch s . . .	162	2e−38
gi|12278481|gb|AAG48981.1|	(AY010994) granule-bound starch . . .	162	2e−38
gi|3493025|gb|AAD02970.1|	(AF079250) granule-bound starch s . . .	161	2e−38
gi|3493091|gb|AAD03003.1|	(AF079283) granule-bound starch s . . .	161	2e−38
gi|3493031|gb|AAD02973.1|	(AF079253) granule-bound starch s . . .	161	2e−38
gi|12278471|gb|AAG48976.1|	(AY010989) granule-bound starch . . .	161	2e−38
gi|12278463|gb|AAG48972.1|	(AY010985) granule-bound starch . . .	161	2e−38
gi|3493067|gb|AAD02991.1|	(AF079271) granule-bound starch s . . .	161	2e−38
gi|3493051|gb|AAD02983.1|	(AF079263) granule-bound starch s . . .	161	2e−38
gi|13774480|gb|AAK38879.1|	(AF353517) granule-bound starch . . .	161	3e−38
gi|12278453|gb|AAG48967.1|	(AY010980) granule-bound starch . . .	161	3e−38
gi|12278449|gb|AAG48965.1|	(AY010978) granule-bound starch . . .	161	3e−38
gi|12278451|gb|AAG48966.1|	(AY010979) granule-bound starch . . .	160	3e−38
gi|13774484|gb|AAK38881.1|	(AF353519) granule-bound starch . . .	160	3e−38
gi|12278427|gb|AAG48954.1|	(AY010967) granule-bound starch . . .	160	3e−38
gi|13774486|gb|AAK38882.1|	(AF353520) granule-bound starch . . .	160	3e−38
gi|12278491|gb|AAG48986.1|	(AY010999) granule-bound starch . . .	160	3e−38
gi|13774482|gb|AAK38880.1|	(AF353518) granule-bound starch . . .	160	4e−38
gi|13377473|gb|AAK20725.1|	(AF318769) granule-bound starch . . .	160	4e−38
gi|3493011|gb|AAD02963.1|	(AF079243) granule-bound starch s . . .	160	4e−38
gi|3493073|gb|AAD02994.1|	(AF079274) granule-bound starch s . . .	160	4e−38
gi|12278479|gb|AAG48980.1|	(AY010993) granule-bound starch . . .	160	5e−38
gi|3493057|gb|AAD02986.1|	(AF079266) granule-bound starch s . . .	160	6e−38
gi|3493087|gb|AAD03001.1|	(AF079281) granule-bound starch s . . .	160	6e−38
gi|3493023|gb|AAD02969.1|	(AF079249) granule-bound starch s . . .	160	6e−38
gi|3493041|gb|AAD02978.1|	(AF079258) granule-bound starch s . . .	160	6e−38
gi|3493109|gb|AAD03012.1|	(AF079292) granule-bound starch s . . .	160	6e−38
gi|3493081|gb|AAD02998.1|	(AF079278) granule-bound starch s . . .	160	6e−38
gi|3493021|gb|AAD02968.1|	(AF079248) granule-bound starch s . . .	160	6e−38
gi|12278457|gb|AAG48969.1|	(AY010982) granule-bound starch . . .	159	6e−38
gi|3493055|gb|AAD02985.1|	(AF079265) granule-bound starch s . . .	159	7e−38
gi|12278477|gb|AAG48979.1|	(AY010992) granule-bound starch . . .	159	7e−38
gi|12278499|gb|AAG48990.1|	(AY011003) granule-bound starch . . .	159	7e−38
gi|12278489|gb|AAG48985.1|	(AY010998) granule-bound starch . . .	159	7e−38
gi|12278511|gb|AAG48996.1|	(AY011009) granule-bound starch . . .	159	8e−38
gi|13774488|gb|AAK38883.1|	(AF353521) granule-bound starch . . .	159	8e−38
gi|12278485|gb|AAG48983.1|	(AY010996) granule-bound starch . . .	159	8e−38
gi|3493095|gb|AAD03005.1|	(AF079285) granule-bound starch s . . .	159	8e−38
gi|12278429|gb|AAG48955.1|	(AY010968) granule-bound starch . . .	159	8e−38
gi|3493077|gb|AAD02996.1|	(AF079276) granule-bound starch s . . .	159	8e−38
gi|3493009|gb|AAD02962.1|	(AF079242) granule-bound starch s . . .	159	8e−38
gi|3493027|gb|AAD02971.1|	(AF079251) granule-bound starch s . . .	159	1e−37
gi|12278413|gb|AAG48947.1|	(AY010960) granule-bound starch . . .	159	1e−37
gi|12278443|gb|AAG48962.1|	(AY010975) granule-bound starch . . .	159	1e−37
gi|3493097|gb|AAD03006.1|	(AF079286) granule-bound starch s . . .	159	1e−37
gi|3493013|gb|AAD02964.1|	(AF079244) granule-bound starch s . . .	159	1e−37
gi|3493015|gb|AAD02965.1|	(AF079245) granule-bound starch s . . .	159	1e−37
gi|3493099|gb|AAD03007.1|	(AF079287) granule-bound starch s . . .	159	1e−37
gi|3493083|gb|AAD02999.1|	(AF079279) granule-bound starch s . . .	159	1e−37
gi|13377475|gb|AAK20726.1|	(AF318770) granule-bound starch . . .	158	1e−37
gi|3493115|gb|AAD03015.1|	(AF079295) granule-bound starch s . . .	158	2e−37
gi|3493017|gb|AAD02966.1|	(AF079246) granule-bound starch s . . .	158	2e−37
gi|12278423|gb|AAG48952.1|	(AY010965) granule-bound starch . . .	158	2e−37
gi|12278425|gb|AAG48953.1|	(AY010966) granule-bound starch . . .	158	2e−37
gi|12278411|gb|AAG48946.1|	(AY010959) granule-bound starch . . .	158	2e−37
gi|12278435|gb|AAG48958.1|	(AY010971) granule-bound starch . . .	158	2e−37
gi|3493035|gb|AAD02975.1|	(AF079255) granule-bound starch s . . .	158	2e−37
gi|12278495|gb|AAG48988.1|	(AY011001) granule-bound starch . . .	158	2e−37
gi|12278501|gb|AAG48991.1|	(AY011004) granule-bound starch . . .	158	2e−37
gi|12278419|gb|AAG48950.1|	(AY010963) granule-bound starch . . .	158	2e−37
gi|12278421|gb|AAG48951.1|	(AY010964) granule-bound starch . . .	158	2e−37
gi|3493037|gb|AAD02976.1|	(AF079256) granule-bound starch s . . .	158	2e−37
gi|3493071|gb|AAD02993.1|	(AF079273) granule-bound starch s . . .	157	2e−37
gi|12278469|gb|AAG48975.1|	(AY010988) granule-bound starch . . .	157	3e−37
gi|3493003|gb|AAD02959.1|	(AF079239) granule-bound starch s . . .	157	3e−37
gi|3493001|gb|AAD02958.1|	(AF079238) granule-bound starch s . . .	157	3e−37
gi|13194734|gb|AAK15529.1|	(AF331953) granule-bound starch . . .	157	3e−37
gi|3493075|gb|AAD02995.1|	(AF079275) granule-bound starch s . . .	157	3e−37

[0405]

Du-1
Accession: AF023159
NID: g3057119
Mol. wt. (calc) = 188101    Residues = 1674
Seq. ID. No. 883
1    M E M V L R S Q S P L C L R S G P V L I F R P T V A G G G G
31   G T Q S L L R T T R F A R R R V I R C V V A S P G C P N R K
61   S R T A S P N V K V A A Y S N Y A P R L L V E S S S K K S E
91   H H D S S R H R E E T I D T Y N G L S G S D A A E L T S N R
121  D V E I E V D L Q H I S E E E L P G K V S I N A S L G E M E
151  T V D E A E V E E D K F E V D T S G I V L R N V A V R E V D
181  P K D E H N A K D V F V V D S S G T A P D N A A V E E V V D
211  E A E V E E D M V D V D I L G L D L N N A T I E E I D L M E
241  E A L L E N F D V D S P G N A S S G R T Y G G V D E L G E L
271  P S T S V D C I A I N G K R R S L K P K P L P I V R F Q E Q
301  E Q I V L S I V D E E G L I A S S C E E G Q P V V D Y D K Q
331  E E N S T A F D E Q K Q L T D D F P E E G I S I V H F P E P
361  N N D I V G S S K F L E Q K Q E L D G S Y K Q D R S T T G L
391  H E Q D Q S V V S S H G Q D K S I V G V P Q Q I Q Y N D Q S
421  I A G S H R Q D Q S I A G A P E Q I Q S V A G Y I K P N Q S
451  I V G S C K Q H E L I I P E P K K I E S I I S Y N E I D Q S
481  I V G S H K Q D K S V V S V P E Q I Q S I V S H S K P N Q S
511  T V D S Y R Q A E S I I G V P E K V Q S I T S Y D K L D Q S
541  I V G S L K Q D E P I I S V P E K I Q S I V H Y T K P N Q S
571  I V G L P K Q Q Q S I V H I V E P K Q S I D G F P K Q D L S
601  I V G I S N E F Q T K Q L A T V G T H D G L L M K G V E A K
631  E T S Q K T E G D T L Q A T F N V D N L S Q K Q E G L T K E
661  A D E I T I I E K I N D E D L V M I E E Q K S I A M N E E Q
691  T I V T E E D I P M A K V E I G I D K A K F L H L L S E E E
721  S S W D E N E V G I I E A D E Q Y E V D E T S M S T E Q D I
751  Q E S P N D D L D P Q A L W S M L Q E L A E K N Y S L G N K
781  L F T Y P D V L K A D S T I D L Y F N R D L S A V A N E P D
811  V L I K G A F N G W K W R F F T E K L H K S E L A G D W W C
841  C K L Y I P K Q A Y R M D F V F F N G H T V Y E N N N N N D
871  F V I Q I E S T M D E N L F E D F L A E E K Q R E L E N L A
901  N E E A E R R R Q T D E Q R R M E E E R A A D K A D R V Q A
931  K V E V E T K K N K L C N V L G L A R A P V D N L W Y I E P
961  I T T G Q E A T V R L Y Y N I N S R P L V H S T E I W M H G
991  G Y N N W I D G L S F A E R L V H H H D K D C D W W F A D V
1021 V V P E R T Y V L D W V F A D G P P G S A R N Y D N N G G H
1051 D F H A T L P N N M T E E E Y W M E E E Q R I Y T R L Q Q E
1081 R R E R E E A I K R K A E R N A K M K A E M K E K T M R M F
1111 L V S Q K H I V Y T E P L E I H A G T T I D V L Y N P S N T
1141 V L T G K P E V W F R C S F N R W M Y P G G V L P P Q K M V
1171 Q A E N G S H L K A T V Y V P R D A Y M M D F V F S E S E E
1201 G G I Y D N R N G L D Y H I P V F G S I A K E P P M H I V H
1231 I A V E M A P I A K V G G L G D V V T S L S R A V Q D L G H
1261 N V E V I L P K Y G C L N L S N V K N L Q I H Q S F S W G G
1291 S E I N V W R G L V E G L C V Y F L E P Q N G M F G V G Y V
1321 Y G R D D D R R F G F F C R S A L E F L L Q S G S S P N I I
1351 H C H D W S S A P V A W L H K E N Y A K S S L A N A R V V F
1381 T I H N L E F G A H H I G K A M R Y C D K A T T V S N T Y S
1411 K E V S G H G A I V P H L G K F Y G I L N G I D P D I W D P
1441 Y N D N F I P V H Y T C E N V V E G K R A A K R A L Q Q K F
1471 G L Q Q I D V P V V G I V T R L T A Q K G I H L I K H A I H
1501 R T L E R N G Q V V L L G S A P D S R I Q A D F V N L A N T
1531 L H G V N H G Q V R L S L T Y D E P L S H L I Y A G S D F I
1561 L V P S I F E P C G L T Q L V A M R Y G T I P I V H K T G G
1591 L F D T V F D V D N D K E R A R D R G L E P N G F S F D G A
1621 D S N G V D Y A L N R A I S A W F D A R S W F H S L C K R V
1651 M E Q D W S W N R P A L D Y I E L Y R S A S K L

[0406]

TABLE XXXI
Maize soluble starch synthase IIb (SSIIb)
Alignments with other similar proteins-Transit Peptide
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                  Sequence                  ending	#
884	MAIZE SSIIb	1	GGIYDNRNGLDYHIPVFGSIAKEP----- P-------MHIVHIAVEMAPIAKVGGLGD	46
885	7489826	1201	GGIYDNRNGLDYHIPVFGSIAKEP---- -P-------MHIVHIAVEMAPIAKVGGLGD	1246
886	9502145	1139	GIYDNRNGMDYHIPVSDSIETEN-------Y-------MRIIHIAVEMAPVAKVGGLGD	1183
887	9502143	1156	GIYDNRNGMDYHIPVSDSIETEN-------Y-------MRIIHIAVEMAPVAKVGGLGD	1200
888	17646328	743	GGIYDNRNGMDYHSPVTDSVAKEP------P-------MHIVHIAVEMAPIAKVGGLGD	788
889	4582789	672	GGVFDNKFGMDYHIPVFGGIVKEP------P-------MHIVHIAVEMAPIAKVGGLGD	717
890	7489274	755	GGIFDNKSGMDYHIPVFGGVAKEP------P-------MHIVHIAVEMAPIAKVGGLGD	800
891	2833389	755	GGIFDNKSGMDYHIPVFGGVAKEP------P-------MHIVHIAVEMAPIAKVGGLGD	800
892	15221083	552	GGIFDNKNGLDYHLPVVGGISKEP-------P-------LHIVHIAVEMAPIAKVGGLGD	597
893	4582783	370	SSATSP-------G-------LYVIHIAAEMAPVAKVGGLGD	397
894	15717885	421	-------LHIAHIAAEMAPVAKVGGLAD	441
895	15236819	545	-------LYVVHIAAEMAPVAKVGGLGD	565
896	18461221	348	VLQVGGLAD	356
897	8901183	129	MPLQGGAAEAPPVERDGNP-------MHIIHITAEMAPIAKVGGLGD	168
898	17227527	1	-------MYIVQIASECAPVIKAGGLGD	21
899	16329217	1	-------MYIVQIASECAPVIKAGGLGD	21
900	2811062	1	-------MKVLFAVSECAPFAKSGGLAD	21
901	18309046	15	EANPFIKTGGLGD	27
902	15903076	1	-------MKILFVAAEGAPFSKTGGLGD	21
903	17229371	1	-------MRILFVAAEAAPIAKVGGMGD	21
904	15900991	1	-------MKILFVAAEGAPFSKTGGLGD	21
905	16124067	1	-------MRVLHVCSELFPLLKTGGLAD	21
906	16080147	1	-------MKILFAVSECTPFVKSGGLAD	21
907	16273270	1	-------MKILHVCSELYPLLKTGGLAD	21
908	15602409	1	-------MKVLHACSELYPLLKTGGLAD	21
909	15672681	2	KER-------K-------MKVLFASSECAPFFKTGGLGD	26
910	17938870	22	-------MRILAVTAEMFPFVKTGGLAD	42
911	16119514	1	-------MRILAVTAEMFPFVKTGGLAD	21
912	15613648	1	-------MNVLHVASECNPFFKTGGLAD	21
913	16331219	1	-------MKILFVAAEVSPLAKVGGMGD	21
914	17366711	1	-------MKVLFASSECAPFFKTGGLGD	21
915	15805621	28	-------P-------MRVLHLASEVFPFSRSGGLGD	49
916	15620537	1	-------MRILFVAAECAPFAKVGGMGD	21
917	15641730	12	EVQGLVKSGGLAD	24
918	7489711	199	GPLAGPN-------V-------MNVVVVASECAPFCKTGGLGD	227
919	15605532	1	-------MKIIHTAIEFAPVIKAGGLGD	21
920	15803938	1	-------MQVLHVCSEMFPLLKTGGLAD	21
921	17367076	1	-------MRVLHLASEVFPFSRSGGLGD	21
922	16766821	1	-------MQVLHVCSEMFPLLKTGGLAD	21
923	16762766	1	-------MQVLHVCSEMFPLLKTGGLAD	21
924	15834801	1	-------MKITHTAIEFAPVIKAGGLGD	21
925	15966599	1	-------MNILSVASEVYPLVKTGGLAD	21
926	15618857	1	-------MRIVQVAVEFTPIVKVGGLGD	21
927	17366350	2	-------IRVLHLAPEAYPLAKVGGLAD	22
928	15384987	138	GPLAGPN-------V-------MNVIVVASECSPFCKTGGLGD	166
929	2129898	245	FESGGEKP-------PPLAGTNVMNIILVSAECAPWSKTGGLGD	281
930	2833384	245	FESGGEKP-------PPLAGTNVMNIILVSAECAPWSKTGGLGD	281
931	15028467	195	GPLAGPN-------V-------MNVIVVASECSPFCKTGGLGD	223
932	15643657	1	-------MKVVFVSYEVFPFAKVGGLAD	21

[0407]

TABLE XXXII
Maize soluble starch synthase III (Du I)
“GLASS” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                     Sequence                     end	#
933	MaizeDuI	47	VVTSLSRAVQDLGH--NVEVIL--PKY----GCL------N--LSNV-------KNL---	80
934	7489826	1247	VVTSLSRAVQDLGH--NVEVIL--PKY----GCL------N--LSNV-------KNL---	1280
935	9502145	1184	VVTSLSRAVQDLGH--TVEVIL--PKY----DCL------N--QSSV-------KDL---	1217
936	9502143	1201	VVTSLSRAIQDLGH--TVEVIL--PKY----DCL------N--QSSV-------KDL---	1234
937	17646328	789	VVTSLSRAVQDLGH--NVEVIL--PKY----DCL------N--LSNV-------KDL---	822
938	4582789	718	VVTSLSRAVQDLNH--NVDIIL--PKY----DCL------N--LSNV-------KDL---	751
939	7489274	801	VVTSLSRAVQDLNH--NVDIIL--PKY----DCL------K--MNNV-------KDF---	834
940	2833389	801	VVTSLSRAVQDLNH--NVDIIL--PKY----DCL------K--MNNV-------KDF---	834
941	15221083	598	VVTSLSRAVQELNH--NVDIVF--PKY----DCI------K--HNFV-------KDL---	631
942	4582783	398	VISGLSKALQKKGH--LVEIIL--PKY----DCM------Q--YDRI-------GDL---	431
943	15717885	442	VISGLGKALQKKGH--LVEIIL--PKY----DCM------Q--VDQV-------SNL---	475
944	15236819	566	VVAGLGKALQRKGH--LVEIIL--PKY----DCM------Q--YDRV-------RDL---	599
945	18461221	357	VVAGLGKALQTKGH--LVEIVL--PKY----DCM------Q--LDQI-------TNL---	390
946	8901183	169	VVTGLAKAALARGH--FVTVML--PFY----ECL------P--KDQI-------EGLKHE	205
947	17227527	22	VVYGLSRELEIRGN--CVELIL--PKY----DCM------R--YDHV-------WGL---	55
948	16329217	22	VIYGLSRELELRGH--CVELIL--PMY----DCM------R--YDHIWGLHDAYRNL---	62
949	2811062	22	VAGALPKELRRLGI--DARVML--PKY----ETIAPEWKKK--MKKV-------AEL---	61
950	18309046	28	VMGALPKELKRKGI--DARVIL--PKY----SAI------K--GELL-------DKL---	61
951	15903076	22	VIGALPKSLVKAGH--EVAVIL--PYY----DMVEAEFGNQ--IEDV-------LEF---	61
952	17229371	22	VVGALPKVLRKMGH--DVRIFL--PYY----GFL------P-------------DKM---	51
953	15900991	22	VIGALPKSLVKAGH--EVAVIL--PYY----DMVEAKFGNQ--IEDV-------LHF---	61
954	16124067	22	VIGALPAAQLAEGA--DVRIIL--PAF----PDL------R--RGIP-------ETV---	55
955	16080147	22	VAGALPKALARLGN--EVAVML--PKY----SQI------P--EPWK-------KEN---	55
956	16273270	22	VLGALPQAQNQIGL--DARFLL--PAY----PAI------T---TGI-------PNT---	54
957	15602409	22	VMGALPFAQKEIGI--DARIVL--PAY----PAI------K---AGI-------PET---	54
958	15672681	27	VAGALPKELAKKSEIDSVAVIL--PYF----KNE------M--KEEY-------RSL---	62
959	17938870	43	ATSALPQALERKGA--DVRTLL--PLY----RGL------TPLVRGR-------KPV---	78
960	16119514	22	ATSALPQALERKGA--DVRTLL--PLY----RGL------TPLVRGR-------KPV---	57
961	15613648	22	VLGSLPKALIKQGI--NVSVIL--PKY----GHL------S--DEWQ-------NQL---	55
962	16331219	22	VVGSLPKVLHQLGH--DVRVFM--PYY----GFI------G-------------DKI---	51
963	17366711	22	VAGALPKELAKKSEIDSVAVIL--PYF----KNE------M--KEEY-------RSL---	57
964	15805621	50	VLGALPAVQARLGE--DAEVTVLSPWY----ASL-----------QG-------EPQ---	82
965	15620537	22	VVGSLPKVLKASAH--DVGIFM--RYY----GFL------------T-------SKL---	51
966	15641730	25	VAKALPQALKALHQ--QVAIAL--PAY----RSV------P--GKED-------AEL---	58
967	7489711	228	VVGALPKALARRGH--RVMVVI--PRY----G--------E--YAEA-------RDL---	259
968	15605532	22	ALYGLAKALA-ANH--TTEVVI--PLY----PKLF-----T--LPKE-------QDL---	55
969	15803938	22	VIGALPAAQIADGV--DARVLL--PAF----PDI------R---RGV-------TDA---	54
970	17367076	22	VLGALPAVQARLGE--DAEVTVLSPWY----ASL-----------QG-------EPQ---	54
971	16766821	22	VIGALPAAQIADGV--DVRVLL--PGF----PDI------R--RGIP-------DAH---	55
972	16762766	22	VIGALPAAQIADGV--DVRVLL--PGF----PDI------R--RGIP-------DAH---	55
973	15834801	22	ALYGLAKALA-VNH--TTEVVI--PLYPKLFTSL------H--EQDL-------FAT---	58
974	15966599	22	VVGALPSALLPHGV--RTRTLV--PGY----PSV------L--KKLK-------KKK---	55
975	15618857	22	AVASLSKELAK-QN--DVEVLL--PHY----PLI------S--KFSS-------SQV---	54
976	17366350	23	VVGALPKALRPLGV--EAHVLL--PWH----GGL------E--ARRV-------GEV---	56
977	15384987	167	VVGALPKALARRGH--RVMVVI--PRY----G--------E--YAEA-------KDL---	198
978	2129898	282	VAGSLPKALARRGH--RVMIVA--PHY----G--------N--YAEA-------HDI---	313
979	2833384	282	VAGSLPKALARRGH--RVMIVA--PHY----G--------N--YAEA-------HDI---	313
980	15028467	224	VVGALPKALARRGH--RVMVVI--PRY----G--------E--YAEA-------KDL---	255
981	15643657	22	VAGTLPKYLKKHGV--DVTIVM--PKH----RIV------E---KNA-------EKFGYE	57
933	maizeDu1	81	---Q-----I-H----Q----SF-----S-----W---G----G----S------EI--N	94
934	7489826	1281	---Q-----I-H----Q----SF-----S-----W---G----G----S------EI--N	1294
935	9502145	1218	---H-----L-Y----Q----SF-----S-----W---G----G----T------EI--K	1231
936	9502143	1235	---H-----L-Y----Q----SF-----S-----W---G----G----T------EI--K	1248
937	17646328	823	---H-----Y-R----Q----SF-----T-----W---G----N----T------EI--K	836
938	4582789	752	---Q-----F-H----K----SY-----F-----W---S----G----T------EI--K	765
939	7489274	835	---R-----F-H----K----NY-----F-----W---G----G----T------EI--K	848
940	2833389	835	---H-----F-H----K----NY-----F-----W---G----G----T------EI--K	848
941	15221083	632	---Q-----F-N----R----SY-----H-----W---G----G----T------EI--K	645
942	4582783	432	---RALDVVI-E----S----YF-----D-----G---Q----L----F------KN--K	450
943	15717885	476	---K-----V-LDVLVQ----SY-----F-----E---G----N----M------FN--N	493
944	15236819	600	---RALDTVV-E----S----YF-----D-----G---K----L----Y------KM--K	618
945	18461221	391	---KVLDVVI-Q----S----YF-----D-----G---N----L----F------SN--N	409
946	8901183	206	CDIE-----V-P----K----GY-----H-----W---D----G----EIRVGPLKT--S	228
947	17227527	56	-----------H----E----AYLNLWVP-----W---F----G----A------AI--H	72
948	16329217	63	---E-----V------------------P-----W---Y----G----S------SIFCD	74
949	2811062	62	---I-----V-----------PV-----G-----W---R----R----Q------YC--G	73
950	18309046	62	---S-----F-K----K----WFMVPV-G-----W---R----N----Q------YC--G	79
952	15903076	62	---E-----V-----------SV-----G-----W---R----R----Q------YC--G	73
952	17229371	52	---E-----I-P----K----DP-----I-----W---K----GYAMFQ------DF--T	69
953	15900991	62	---E-----V-----------SV-----G-----W---R----R----Q------YC--G	73
954	16124067	56	---L-----V-R----E----ID-----T-----F---A----G-----------RV--A	68
955	16080147	56	---K-----K-Q----AECTVAV-----G-----W---R----Q----Q------YC--G	73
956	16273270	55	---Q-----V-V----A----EF-----D-----N---F----A----G------HV--V	68
957	15602409	55	---T-----V-V----S----EF-----D-----N---F----A----G------HI--V	68
958	15672681	63	---L-----K-D----E----FY-----DFVDVGW---R----H----E------YV--G	81
959	17938870	79	---L-----V-A----N----IL-----E-------------------Q------PV--S	89
960	16119514	58	---L-----V-A----N----IL-----E-------------------Q------PV--S	68
961	15613648	56	---T-----L-K----K----SF-----T-----V---S----V----T------WR--N	69
962	16331219	52	---D-----V-P----K----EP-----V-----W---K----G----------------	61
963	17366711	58	---L-----K-D----E----FY-----DFVDVGW---R----H----E------YV--G	76
964	15805621	83	---E-----L-W----R----GE-----A-----W---G----E---------------Q	93
965	15620537	52	---D-----IPA----E----PI-----W-----W---G----Y----A------MF--N	66
966	15641730	59	---V-----L-E----T----EL-----TH----W---P----H----T------QY--R	73
967	74897112	60	---G-----V-R----R----RY-----K-----V---A----GQD--S------EV--T	275
968	15605532	56	---C-----S-I----Q----KL-----S-----YFFAG----E----Q------EA--T	72
969	15803938	55	---Q-----V-V----S----RR-----D-----T---F----A----G------HI--T	68
970	17367076	55	---E-----L-W----R----GE-----A-----W---G----E---------------Q	65
971	16766821	56	---V-----V-S----R----RD-----T-----F---A----G-----------KI--S	68
972	16762766	56	---V-----V-S----R----RD-----T-----F---A----G-----------KI--S	68
973	15834801	59	---Q-----K-I----P----YF-----F-----A---G----E----Q------EA--T	72
974	15966599	56	---p-----V-G----R----FD-----N-----L---F----G----H------PA--T	69
975	15618857	55	---L-----S-E----R----SF-----Y-----YEFLG----K----Q------QA--S	71
976	17366350	57	---------------------AF-----A-----F---F----G----R------EE--R	66
977	15384987	199	---G-----V-R----K----RY-----R-----V---A----GQD--S------EV--S	214
978	2129898	314	---G-----V-R----K----RY-----K-----V---A----G----QDM----EV--T	329
979	2833384	314	---G-----V-R----K----RY-----K-----V---A----G----QDM----EV--T	329
980	15028467	256	---G-----V-R----K----RY-----R-----V---A----GQD--S------EV--S	271
981	15643657	58	IK-K-----V-A----E----SL-----S-----V---SHVKTD----Q------KF--D	77
933	Maize Du1	95	---VWRG-LV----EG--LCV--Y--F--LE-----P-----------Q---NG---M--	114
934	7489826	1295	---VWRG-LV----EG--LCV--Y--F--LE-----P-----------Q---NG---M--	1314
935	9502145	1232	---VWVG-RV----ED--LTV--Y--F--LE-----P-----------Q---NG---M--	1251
936	9502143	1249	---VWVG-RV----ED--LTV--Y--F--LE-----P-----------Q---NG---M--	1268
937	17646328	837	---VWFG-KV----ED--VPV--Y--F--LE-----P-----------Q---NG---M--	856
938	4582789	766	---VWHG-KV----EG--LSV--Y--F--LE-----P-----------Q---NG---LF-	786
939	7489274	849	---VWFG-KV----EG--LSV--Y--F--LE-----P-----------Q---NG---LF-	869
940	2833389	849	---VWFG-KV----EG--LSV--Y--F--LE-----P-----------Q---NG---LF-	869
941	15221083	646	---VWHG-KV----EG--LSV--Y--F--LD-----P-----------Q---NG---LF-	666
942	4582783	451	---IWVG-TV----EG--LPV--Y--F--IE-----P-----------H---HPGKFF--	473
943	15717885	494	K--IWTG-TV----EG--LPV--Y--F--IE-----P-----------Q---HPAMFF--	517
944	15236819	619	---IWIG-TV----EG--LPV--H--F--IE-----P-----------Q---HPSKFF--	641
945	18461221	410	---VWTG-TV----EG--LPV--Y--F--IE-----P-----------Q---HPSKFF--	432
946	8901183	229	---VFWG-RV----QG--CPV--Y--L--IK-----P-----------A---DD---TNC	250
947	17227527	73	CT-VYCG-WV----HG--RVC--F--F--IE-----P-----------HSEDNF---F--	97
948	16329217	75	---VFCG-WV----HG--RLC--F--F--IQ-----P-----------KSSDNF---F--	97
949	2811062	74	---VEEL-RH----DG--VIY--Y--F--ID-----N-----------E---YY---F--	93
950	18309046	80	---VYQC-EY----DE--VIY--Y--L--LD-----S-----------E---FY---F--	99
951	15903076	74	---IKKT-VL----NG--VTF--Y--F--ID-----N-----------Q---YY---F--	93
952	17229371	70	---VHEA-VL----PG--TDVP-L--Y--LF-----G-----------H---PA---F--	90
953	15900991	74	---IKKT-VL----NG--VTF--Y--F--ID-----N-----------Q---YY---F--	93
954	16124067	69	---LRYG-HY----RG--IGI--Y--L--ID-----APALYDRAGSPYH---DA---S--	99
955	16080147	74	---IEHM-AE----ND--VNY--Y--F--ID-----N-----------E---YY---F--	93
956	16273270	69	---LRYG-EY----NG--VGI--Y--L--ID-----A-----------P---HL---Y--	88
957	15602409	69	---LRYG-EY----KG--LGV--Y--L--IDA----P-----------H---LY---Q--	89
958	15672681	82	---VKSL-EK----EG--VKY--Y--F--LD-----N-----------E---HY---F--	101
959	17938870	90	---VYYV-VS----DG--HKL--L--L--LE-----A-----------A---SL---F--	109
960	16119514	69	---VYYV-VS----DG--HKL--L--L--LE-----A-----------A---SL---F--	88
961	15613648	70	---QYCG-LEQFVDQG--VTY--Y--F--ID-----N-----------E---YY---F--	93
962	16331219	62	-----EA-MF----QQ--FAV--YQSY--L------P-----------D---TK---I--	80
963	17366711	77	---VKSL-EK----EG--VKY--Y--F--LD-----N-----------E---HY---F--	96
964	15805621	94	---AWLG-EL----RQ--DGV--R--Y--LF-----L-----------G---LN---E--	113
965	15620537	67	HFAVYET-QL----PG--SDVP-L--Y--LM-----G-----------H---PA---9--	90
966	15641730	74	---VLKR-DL----DG--VPI--Y--L--IDCPAYFD-----------R---PA---L--	98
967	7489711	276	---YFHS-YI----DG--VDF--V--F--VE-----APPF--------R---HR---H--	298
968	15605532	73	---AFSY-FY----EG--IKVTLF--K--LD-----T-----------Q---PE---L--	94
969	15803938	69	---LLFG-HY----NG--VGI--Y--L--ID-----A-----------P---H-------	86
970	17367076	66	---AWLG-EL----RQ--DGV--R--Y--LF-----L-----------G---LN---E--	85
971	16766821	69	---LLFG-HY----NG--VGI--Y--L--IDA----P-----------H---LY---E--	89
972	16762766	69	---LLWG-HY----NG--VGI--Y--L--IDA----P-----------H---LY---S--	89
973	15834801	73	---AFSY-FY----EG--IKV--T--LLKLD-----S-----------Q---PE---L--	94
974	15966599	70	---VLAA-EV----NG--VDL--L--V--LD-----Q-----------P---AL---Y--	89
975	15618857	72	---AISY-SY----EGLTLTI--I--T--LD-----S-----------Q---IE---L--	93
976	17366350	67	---APLGERV----EG--GVR--F--L--LL-----G-----------V---EG---F--	87
977	15384987	215	---YFHA-FI----DG--VDF--V--F--LE-----APPF--------R---HR---H--	237
978	2129898	330	---YFHT-YI----DG--VDI--V--F--ID-----SPIF--------R---NL---E--	352
979	2833384	330	---YFHT-YI----DG--VDI--V--F--ID-----SPIF--------R---NL---E--	352
980	15028467	272	---YFHA-FI----DG--VDF--V--F--LE-----APPF--------R---HR---H--	294
981	15643657	78	---IYES-VL----PG--SDVKTY--F--VA-----N-----------D---YY---F--	99
933	maizeDu-1	115	----XXXXXXXXXXXXXXXXXXCR---------SA--------L--------E---FL-L	141
934	7489826	1315	----FGVGYVYGRDDDRRFGFFCR---------SA--------L--------E---FL-L	1341
935	9502145	1252	----FGVGCVYGRNDDRRFGFFCH---------SA--------L--------E---FI-L	1278
936	9502143	1269	----FGVGCVYGRNDDRRFGFFCH---------SA--------L--------E---FI-L	1295
937	17646328	857	----FWVGCVYGRNDESRFGFFCH---------SA--------L--------E---FL-R	883
938	4582789	787	----NVGCVYGRANDAERFGFFCH---------AA--------L--------E---FL-L	813
939	7489274	870	----SKGCVYGCSNDGERFGFFCR---------AA--------L--------E---FL-L	896
940	2833389	870	----SKGCVYGCSNDGERFGFFCH---------AA--------L--------E---FL-L	896
941	15221083	667	----QRGCVYGCADDAGRFGFFCH---------AA--------L--------E---FL-L	693
942	4582783	474	----WRGDYYGAHDDFRRFSYFSR---------AA--------L--------E---FL-L	500
943	15717885	518	----SRAQYYGEHDDFKRFSYFSR---------AA--------L--------E---LL-Y	544
944	15236819	642	----WRGQFYGEQDDFRRFSYFSR---------AA--------L--------E---LL-L	668
945	18461221	433	----WRAQYYGEHDDFKRYSYFSR---------AA--------L--------E---LL-Y	459
946	8901183	251	NIFRGGRIYGGSYNEMEAYLYFCR---------AC--------L--------E---YL-N	281
947	17227527	98	----NRGCYYGCDDDDMRFAFFSK---------AA--------L--------E---FL-H	124
948	16329217	98	----NRGHYYGALDDHMRFAFFSK---------AA--------M--------E---FL-L	124
949	2811062	94	----KRPQLYGHYDDGERFAYFCR---------AV--------L--------E---VL-P	120
982	7484399	62	CR---------AC--------L--------E---YLNV	71
950	18309046	100	----HRNGLYGEGDDGERFAFFDR---------AV--------L--------E---TL-K	126
951	15903076	94	----FRGHVYGDFDDGERFAFFQL---------AA--------I--------E---AM-E	120
952	17229371	91	----TPRRIYSGDDEDWRFTLFSN---------GA--------A--------E---FC-W	117
953	15900991	94	----FRGHVYGDFDDGERFAFFQL---------AA--------I--------E---AM-E	120
954	16124067	100	----LYAYSDNYLRFALLGWMACE---------LA--------C--------G------L	124
955	16080147	94	----NRDSLYGHYDDGERFAFFSR---------AV--------L--------E---AA-K	120
956	16273270	89	----GREGNPYHDAYYNDYGDNYKRFALLGWVGAE--------L--------A---TG-L	124
957	15602409	90	----REGNPYHDQWYNDYADNYKR---------FALLGWVAAEL--------A---TG-L	124
958	15672681	102	----GRGQLYGYGDDGERFAFFDL---------AL--------C--------Q---LL-E	128
959	17938870	110	----DRDGHPYGVKGEPFADNDLR---------FA--------VLSKVAA--E---IA-L	142
960	16119514	89	----DRDGHPYGVKGEPFADNDLR---------FA--------VLSKVAA--E---IA-L	121
961	15613648	94	----KRERLYGYLDEAERFTFFNH---------AV--------L--------S---SL-P	120
962	16331219	81	----PLYLFGHPAFDSRRIYGGDD---------EA--------W--------R---FT-F	107
963	17366711	97	----GRGQLYGYGDDGERFAFFDL---------AL--------C--------Q---LL-E	123
964	15805621	114	----FQRPGLYHPDDVERFCAFGR---------AA--------L--------P---AL-D	140
965	15620537	91	----DPHRIYSGEDEDWRFTFFAN---------GA--------A--------E---FS-W	117
966	15641730	99	----YAENNQAYADNGERFGFFSA---------AC--------L--------D---VL-P	125
967	7489711	299	----NNIYGGERLDILKRMILFCK---------AA--------V--------E---VP-W	325
968	15605532	95	----FENAETIYTSDDAFRFCAFS---------AA--------A--------A---SY-I	121
969	15803938	87	-----LYDRPGSPYHDTNLFAYTD---------NV--------L--------R---FA-L	112
970	17367076	86	----FQRPGLYHPDDVERFCAFGR---------AA--------L--------P---AL-D	112
971	16766821	90	----RPGSPYHDTNLYAYTDNVLR---------FA--------LLGWVGCEMA---CG-L	124
972	16762766	90	----RPGSPYHDTNLYAYIDNVLR---------FA--------LLGWVGCEMA---CG-L	124
973	15834801	95	----FEEAETIYTNDDAFRFCAFS---------AA--------A--------A---AY-I	121
974	15966599	90	----ARDGGPYLDSTGRDYPDNFR---------RF--------A--------A---LS-L	116
975	15618857	94	----FSTTSVYSENNVVRFSAFAA---------AA--------A--------A---Y--L	119
976	17366350	88	----GRERVYGYPDDAERYLRFAL---------AA--------K--------E---V---	112
977	15384987	238	----NDIYGGERFDVLKRMILFCK---------AA--------V--------EVPWFA-P	267
978	2129898	353	----SNIYGGNRLDILRRMVLFCK---------AA--------V--------E---VP-W	379
979	2833384	353	----SNIYGGNRLDILRRMVLFCK---------AA--------V--------E---VP-W	379
980	15028467	295	----NDIYGGERFDVLKRMILFCK---------AA--------V--------EVPWFA-P	324
981	15643657	100	----SAEDVYAGPDLGEQAIFFCA---------AT--------L--------D---LV-K	126
933	MaizeDu1	142	--------QS-------GS----------S---------PNIIH-CNDWSS--A--PV-A	161
934	7489826	1342	--------QS-------GS----------S---------PNIIH-CHDWSS--A--PV-A	1361
935	9502145	1279	--------QN-------EF----------S---------PHIIH-CHDWSS--A--PV-A	1298
936	9502143	1296	--------QN-------EF----------S---------PHIIH-CHDWSS--A--PV-A	1315
937	17646328	884	--------QN-------GS----------S---------PDIIH-CHDWSS--A--PV-A	903
938	4582789	814	--------QN-------GS----------H---------PDIIH-CHDWSS--A--PV-A	833
939	7489274	897	--------QG-------GF----------S---------PDIIH-CHDWSS--A--PV-A	916
940	2833389	897	--------QG-------GF----------S---------PDIIH-CHDWSS--A--PV-A	916
942	15221083	694	--------QG-------GF----------H---------PDILH-CHDWSS--A--PV-S	713
942	4582783	501	--------QA-------GK----------K---------PDIIH-CHDWQT--A--FI-A	520
943	15717885	545	--------QS-------GK----------K---------VDIIH-CHDWQT--A--FV-A	564
944	15236819	669	--------QS-------GK----------K---------PDIIH-CHDWQT--A--FV-A	688
945	18461221	460	--------QS-------GK----------K---------IDIIH-CHDWQT--A--FV-A	479
946	8901183	282	--------VS-------QQ----------N---------PHVLQ-LHDWHA--A--AA-S	301
947	17227527	125	--------QS-------NK----------R---------PDIIH-CHDWQT--G--LI-P	144
948	16329217	125	--------RS-------NK----------R---------PDIIH-CHDWQT--G--LV-P	144
949	2811062	121	--------EI-------QF----------Q---------PDVIH-CHDWHT--G--MV-P	140
982	7484399	72	--------SQ-------QQ----------N---------PHVLQ-LHDWHA--A--AA-S	91
950	18309046	127	--------EI-------DW----------C---------PDIIH-CNDWQT--G--MI-P	146
951	15903076	121	--------RI-------DF----------I---------PDLLH-VHDYHT--A--MI-P	140
952	17229371	118	--------NY-------W-----------K---------PDIIH-CHDWHT--G--MIPV	137
953	15900991	121	--------RI-------DF----------I---------PDLLH-VHDYHT--A--MI-P	140
954	16124067	125	--------DG-------YW----------R---------PEVVH-AHDWHA--G--LT-C	144
955	16080147	121	--------VV-------NV----------Q---------ADIVH-THDWHT--A--MV-N	140
956	16273270	125	--------DS-------WW----------R---------AEVVH-AHDWHA--GL-CV-A	145
957	15602409	125	--------DS-------WW----------H---------ADVVH-AHDWHAGLA--SA-Y	146
958	15672681	129	--------KL-------DF----------I---------PDVLH-VNDWQT--A--MV-P	148
959	17938870	143	--------GAID-----GW----------Q---------PDVVH-VHDWHA--A--LT-C	164
960	16119514	122	--------GAID-----GW----------Q---------PDVVH-VHDWHA--A--LT-C	143
961	15613648	121	--------FL-------DE----------S---------PDLIH-CHDWQS--G--LI-P	140
962	16331219	108	--------FS-------NG----------AAEFAWNHWKPEIIH-CHDWHT--G--MIPV	137
963	17366711	124	--------KL-------DF----------I---------PDVLH-VNDWQT--A--MV-P	143
964	15805621	141	--------AV-------GV----------T---------PDVLH-GHDWQA--G--LV-V	160
965	15620537	118	--------NY-------W-----------K---------PQVIH-CHDWHT--G--MIPV	137
966	15641730	126	--------KL-------GI----------Q---------PDIIH-ANDWHT--G--LV-P	145
967	7489711	326	--------YAPCGGTVYGD----------G---------NLVFI-ANDWHT--A--LL-P	352
968	15605532	122	--------QK--------E----------G---------ANIVH-LHDWHT--G--LV-A	140
969	15803938	113	LGWVGAEMAS-------GL----------DPFWR-----PDVVH-AHDWHAGLA--PA-Y	146
970	17367076	113	--------AV-------GV----------T---------PDVLH-GHDWQA--G--LV-V	132
971	16766821	125	--------DP-------FW----------R---------PDVVH-AHDWHAGLA--PA-Y	146
972	16762766	125	--------DP-------FW----------R---------PDVVH-AHDWHAGLA--PA-Y	146
973	15834801	122	--------QK--------E----------G---------ADIVH-LHDWHV--G--LV-A	140
974	15966599	117	--------AA-------AEIAGNGIIPNWK---------PDIVH-VHDWQT--ALTPV--	147
975	15618857	120	--------QE-------AD----------P---------ADIVH-LHDWHV--G--LL-A	139
976	17366350	113	-----------------AR----------G---------YDLVH-AHDWTA--A--LL-A	130
977	15384987	268	--------CG-------GSIYG-------D---------GNLVFIANDWHT--A--LL-P	291
978	2129898	380	--------HVPCGGICYGD----------G---------NLVFI-ANDWHT--A--LL-P	406
979	2833384	380	--------HVPCGGICYGD----------G---------NLVFI-ANDWHT--A--LL-P	406
980	15028467	325	--------CG-------GSIYG-------D---------GNLVFIANDWHT--A--LL-P	348
981	15643657	127	--------HL-------DL----------K---------PDIVH-VNDWQT--A--LI-P	146
933	Maize Du1	162	WLHKENYA-KSS-L-A-N-ARVV---------------FTIHN-----------------	184
934	7489826	1362	WLHKENYA-KSS-L-A-N-ARVV---------------FTIHN-----------------	1384
935	9502145	1299	WLYKEHYS-QSR-M-A-S-TRVV---------------FTIHN-----------------	1321
936	9502143	1316	WLYKEHYS-QSR-M-A-S-TRVV---------------FTIHN-----------------	1338
937	17646328	904	WLFKEQYA-QNG-L-S-N-GRVV---------------FTIHN-----------------	926
938	4582789	834	WLFKEQYT-HYG-L-S-K-ARVV---------------FTIHN-----------------	856
939	7489274	917	WLFKEQYT-HYG-L-S-K-SRIV---------------FTIHN-----------------	939
940	2833389	917	WLFKEQYT-HYG-L-S-K-SRIV---------------FTIHN-----------------	939
941	15221083	714	WLFKDHYT-QYG-L-I-K-TRIV---------------FTIHN-----------------	736
942	4582783	521	PLYWDVYA-FKG-L-N-S-ARIC---------------FTCHN-----------------	543
943	15717885	565	PLYWDVYA-NLG-F-N-S-ARIC---------------FTCHN-----------------	587
944	15236819	689	PLYWDLYA-PKG-L-D-S-ARIC---------------FTCHN-----------------	711
945	18461221	480	PLYWDIYA-TRG-F-S-S-ARIC---------------FTCHN-----------------	502
946	8901183	302	MLYWDVYN-PNG-F-S-R-TRLM---------------LTIHN-----------------	324
947	17227527	145	VMLYEIYK-YHG-M-D-T-QRVC---------------YTIHN-----------------	167
948	16329217	145	VLLYEIYR-FHG-M-D-H-QRVC---------------YTIHN-----------------	167
949	2811062	141	FLLREQYR-HEL-FYV-D-MRTV---------------FTIHN-----------------	164
982	7484399	92	MLYWDVYN-PNG-F-S-R-TRLM---------------LTIHN-----------------	114
950	18309046	147	VLHKLEYS-KDP-FYK-N-IKTV---------------TSIHN-----------------	170
951	15903076	141	FLLKEKYRWIQA-Y-E-D-IETV---------------LTIHN-----------------	164
952	17229371	138	WMNQS----------P-D-ITTV---------------FTIHN-----------------	153
953	15900991	141	FLLKEKYRWIQA-Y-E-D-IETV---------------LTIHN-----------------	164
954	16124067	145	-----AYL-AAR-G-R-P-ARSV---------------FTVHN-----------------	162
955	16080147	141	YLLKEEYR-KHP-FYE-R-MKSV---------------LTIHN-----------------	164
956	16273270	146	YLFNKGKP-----------AKSV---------------FTIHN-----------------	162
957	15602409	147	LFNKGRPA-KS-----------V---------------FTIHN-----------------	162
958	15672681	149	FLLKEKYNWIKA-Y-E-K-IKTV---------------LTIHN-----------------	172
959	17938870	165	V-----YL-ADS-T-P-S-VASV---------------LTLHN-----------------	182
960	16119514	144	V-----YL-ADS-T-P-S-VASV---------------LTLHN-----------------	161
961	15613648	141	AYMKTGSV-ENP-V-P-----TV---------------FTIHNLRYQGAFPPDVFREL--	175
962	16331219	138	WMHQS----------P-D-IATV---------------FTIHN-----------------	153
963	17366711	144	FLLKEKYNWIKA-Y-E-K-IKTV---------------LTIHN-----------------	167
964	15805621	161	-------A-HAR-L-R-G-LRTA---------------FSVHN-----------------	176
965	15620537	138	WMHQS----------P-D-ISTV---------------FTIHN-----------------	153
966	15641730	146	FLLKTRYR-YDSFF-E-Q-VKSV---------------LTVHNAIFKGIFSYHQLEVIPE	186
967	7489711	353	VYLKAYYR-DNG-L-M-QYARSV---------------LVIHN-----------------	376
968	15605532	141	GLLKQQPC--SQ-L-----QKIV---------------LTLHN-----------------	160
969	15803938	147	LAARGRPA-KS-----------V---------------FTVHN-----------------	162
970	17367076	133	-------A-HAR-L-R-G-LRTA---------------FSVHN-----------------	148
971	16766821	147	LAARGRPA-KS-----------V---------------FTVHN-----------------	162
972	16762766	147	LAARGRPA-KS-----------V---------------FTVHN-----------------	162
973	15834801	141	GLLKQQPC-PQL-------QKIV---------------LTLHN-----------------	160
974	15966599	148	------YM-RFG-P-APD-LPTV---------------MTIHN-----------------	165
975	15618857	140	GLLKNPL----N-P-V-H-SKIV---------------FTIHN-----------------	159
976	17366350	131	LYAPTVYT-IHN-L-A-H-QGLVDPGLFFSWTGLPWSLFHMEA-----------------	168
977	15384987	292	VYLKAYYR-DNG-L-M-QYTRSV---------------LVIHNIAHQGRGPVDDFAT---	329
978	2129898	407	VYLKAYYR-DHG-L-M-NYTRSV---------------LVIHN-----------------	430
979	2833384	407	VYLKAYYR-DHG-L-M-NYTRSV---------------LVIHN-----------------	430
980	15028467	349	VCLKAYYR-DNG-L-M-QYTRSV---------------LVIHNIAHQGRGPVDDFAT---	386
981	15643657	147	VYLKTVYR-DDPYF-S-R-TATV---------------LTIHN-----------------	170
933	maizeDu1	185	--------L-----------------E---------------------------------	186
934	7489826	1385	--------L-----------------E---------------------------------	1386
935	9502145	1322	--------L-----------------E---------------------------------	1323
936	9502143	1339	--------L-----------------E---------------------------------	1340
937	17646328	927	--------L-----------------E---------------------------------	928
938	4582789	857	--------L-----------------E---------------------------------	858
939	7489274	940	--------L-----------------E---------------------------------	941
940	2833389	940	--------L-----------------E---------------------------------	941
941	15221083	737	--------L-----------------E---------------------------------	738
942	4582783	544	--------L-----------------E---------------------------------	545
943	15717885	588	--------L-----------------E---------------------------------	589
944	15236819	712	--------L-----------------E---------------------------------	713
945	18461221	503	--------L-----------------E---------------------------------	504
946	8901183	325	--------L-----------------D---------------------------------	326
947	17227527	168	--------F-----------------KHQGIGGVKTLWATGLNREAYYFQDDKLRDDHNP	202
948	16329217	168	--------F-----------------E---------------------------------	169
949	2811062	165	--------L-----------------E---------------------------------	166
982	7484399	115	--------L-----------------E---------------------------------	116
950	18309046	171	--------L-----------------E---------------------------------	172
951	15903076	165	--------L-----------------E---------------------------------	166
952	17229371	154	--------L-----------------E---------------------------------	155
953	15900991	165	--------L-----------------E---------------------------------	166
954	16124067	163	--------L-----------------AYQGL-----------------------------	168
955	16080147	165	--------L-----------------E---------------------------------	166
956	16273270	163	--------LAYQG-------------Q---------------------------------	168
957	15602409	163	--------LAYQG-------------Q---------------------------------	168
958	15672681	173	--------I-----------------E---------------------------------	174
959	17938870	183	--------L-----------------A---------------------------------	184
960	16119514	162	--------L-----------------A---------------------------------	163
961	15613648	176	--------L-----------------H---------------------------------	177
962	16331219	154	--------L-----------------A---------------------------------	155
963	17366711	168	--------I-----------------E---------------------------------	169
964	15805621	177	--------L-----------------QYQGRWNLHEAAGWTGLPDWTFGPDGVEF-----	206
965	15620537	154	--------L-----------------A---------------------------------	155
966	15641730	187	LNLSGMEFL-----------------Q---------------------------------	196
967	7489711	377	--------IAHQGRGPVDDFVNF---D---------------------------------	392
968	15605532	161	--------F-----------------G---------------------------------	162
969	15803938	163	--------L-----------------AYQGM-----------------------------	168
970	17367076	149	--------L-----------------QYQGRWNLHEAAGWTGLPDWTFGPDGVEF-----	178
971	16766821	163	--------L-----------------A---------------------------------	164
972	16762766	163	--------L-----------------A---------------------------------	164
973	15834801	161	--------FGYRGYTTREVLEASSLNE---------------------------------	179
974	15966599	166	--------IAFQG-------------Q---------------------------------	171
975	15618857	160	--------FGYRGYCSTQLLAASQIDD---------------------------------	178
976	17366350	169	--------L-----------------E---------------------------------	170
977	15384987	330	--------M-----------------D---------------------------------	331
978	2129898	431	--------I-----------------AHQGRGPVEDFNTVDLSGNYLDLFKMYDP-----	460
979	2833384	431	--------I-----------------AHQGRGPVEDFNTVDLSCNYLDLFKMYDP-----	460
980	15028467	387	--------M-----------------D---------------------------------	388
981	15643657	171	--------L-----------------G---------------------------------	172

[0408]

TABLE XXXIII
Maize soluble starch synthase III (Du I)
“LINKR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                     Sequence                     end	#
983	MaizeDuI	187	F-----------------------------GA----------------------------	189
984	7489826	1387	F-----------------------------GA----------------------------	1389
985	9502145	1324	F-----------------------------GA----------------------------	1326
986	9502143	1341	F-----------------------------GA----------------------------	1343
987	17646328	929	F-----------------------------GA----------------------------	931
988	4582789	859	F-----------------------------GA----------------------------	861
989	7489274	942	F-----------------------------GA----------------------------	944
990	2833389	942	F-----------------------------GA----------------------------	944
991	15221083	739	F-----------------------------GA----------------------------	741
992	4582783	546	YQGTAGASELEACGLDSHQLNRPDRMQDN-SA----------------------------	576
993	15717885	590	YQGTAPARDLAWCGL---------------DV----------------------------	606
994	15236819	714	YQGTA-------------------------SA----------------------------	720
995	18461221	505	Y-----------------------------QG----------------------------	507
996	8901183	327	N-----------------------------TG----------------------------	329
997	17227527	203	F-----------------------------AL----------------------------	205
998	16329217	170	HQGIA-------------------------GANIL-------------------------	179
999	2811062	167	F-----------------------------QGLFPRGILEDLLNLDGRYFTVDHLEFYGC	197
1000	7484399	117	N-----------------------------TG----------------------------	119
1001	18309046	173	FQGNFSADVLPELFGYDYEPVRNGSLEFYGGM----------------------------	204
1002	15903076	167	F-----------------------------QG----------------------------	169
1003	17229371	156	YQGPWRWYLDKITWCPWYMQ----------GH----------------------------	177
1004	15900991	167	F-----------------------------QG----------------------------	169
1005	16124067	169	F-----------------------------SA----------------------------	171
1006	16080147	167	F-----------------------------QGIFPPDVT---------------------	176
1007	16273270	169	F-----------------------------SY----------------------------	171
1008	15602409	169	F-----------------------------AY----------------------------	171
1009	15672681	175	F-----------------------------QG----------------------------	177
1010	17938870	185	FQ----------------------------GQ----------------------------	188
1011	16119514	164	FQ----------------------------GQ----------------------------	167
1012	15613648	178	F-----------------------------AP----------------------------	180
1013	16331219	156	YQGPWRGLLETMTWCPWYMQ----------GD----------------------------	177
1014	17366711	170	F-----------------------------QG----------------------------	172
1015	15805621	207	F-----------------------------GD----------------------------	209
1016	15620537	156	YQGPWRWKLEKITWCPWYMQ----------GD----------------------------	177
1017	15641730	197	Y-----------------------------GH----------------------------	199
1018	7489711	393	L-----------------------------PE----------------------------	395
1019	15605532	163	Y-----------------------------RG----------------------------	165
1020	15803938	169	F-----------------------------YA----------------------------	171
1021	17367076	179	F-----------------------------GD----------------------------	181
1022	16766821	165	Y-----------------------------QG----------------------------	167
1023	16762766	165	Y-----------------------------QG----------------------------	167
1024	15834801	180	F-----------------------------YL----------------------------	182
1025	15966599	172	F-----------------------------GA----------------------------	174
1026	15618857	179	F-----------------------------HL----------------------------	181
1027	17366350	171	FY----------------------------GR----------------------------	174
1028	15384987	332	L-----------------------------PE----------------------------	334
1029	2129898	461	V-----------------------------GG----------------------------	463
1030	2833384	461	V-----------------------------GG----------------------------	463
1031	15028467	389	L-----------------------------PE----------------------------	391
1032	15643657	173	YQGVFDPKYLSFAGLPDYVYTID-------GL----------------------------	197
983	maizeDu1	190	-H------------------------H-----------------------------I---	192
984	7489826	1390	-H------------------------H-----------------------------I---	1392
985	9502145	1327	-H------------------------Y-----------------------------I---	1329
986	9502143	1344	-H------------------------Y-----------------------------I---	1346
987	17646328	932	-H------------------------H-----------------------------I---	934
988	4582789	862	-N------------------------L-----------------------------I---	864
989	7489274	945	-D------------------------L-----------------------------I---	947
990	2833389	945	-D------------------------L-----------------------------I---	947
991	15221083	742	-N------------------------A-----------------------------I---	744
992	4582783	577	-H------------------------NRVNS-------------------------V---	583
993	15717885	607	-E------------------------H-----------------------------L---	609
994	15236819	721	-S------------------------E-----------------------------L---	723
995	18461221	508	-T------------------------A-----------------------------P---	510
996	8901183	330	-ETRQDEFFFTGVPGENFA-------T-----------------------------I---	349
997	17227527	206	-N------------------------Y-----------------------------M---	208
998	16329217	180	-H------------------------A-----------------------------T---	182
999	2811062	198	VS------------------------F-----------------------------M---	201
1000	7484399	120	-ETRQDEFFFTGVPGENFA-------T-----------------------------I---	139
1001	18309046	205	-S------------------------F-----------------------------M---	207
1002	15903076	170	-Q------------------------FSEGMLGDLFGVGFERYADGTLRWNNCLNWM---	201
1003	17229371	178	-N------------------------T-----------------------------M---	180
1004	15900991	170	-Q------------------------FSEGMLGDLFGVGFERYADGTLRWNNCLNWM---	201
1005	16124067	172	-D------------------------H-----------------------------L---	174
1006	16080147	177	-H------------------------DL----------------------------L---	180
1007	16273270	172	-H------------------------HLYEIGLPTGMFHVEGLELFGQISY-----L---	198
1008	15602409	172	-H------------------------HLVEIGLPAGMFHVDGLELHGQISY-----L---	198
1009	15672681	178	-L------------------------M-----------------------------H---	180
1010	17938870	189	-Y------------------------P-----------------------------L---	191
1011	16119514	168	-Y------------------------P-----------------------------L---	170
1012	15613648	181	-E------------------------HFAGLEMDGAINF-----------------L---	195
1013	16331219	178	-N------------------------V-----------------------------M---	180
1014	17366711	173	-L------------------------M-----------------------------H---	175
1015	15805621	210	-L------------------------N-----------------------------L---	212
1016	15620537	178	-S------------------------T-----------------------------M---	180
1017	15641730	200	-D------------------------H-----------------------------V---	202
1018	7489711	396	-H------------------------Y-----------------------------IDHF	401
1019	15605532	166	-Y------------------------TTREILEASSLNEFYISQYQLFRDPQTCV-L---	196
1020	15803938	172	-H------------------------H-----------------------------M---	174
1021	17367076	182	-L------------------------N-----------------------------L---	184
1022	16766821	168	-M------------------------F-----------------------------Y---	170
1023	16762766	168	-M------------------------F-----------------------------Y---	170
1024	15834801	183	-S------------------------HYQLFRDPQTCV------------------L---	196
1025	15966599	175	-SVFPELALPPDAFSTQFVEYYGDVGF-----------------------------L---	201
1026	15618857	182	-S------------------------HYQLFRDPQTSV------------------L---	195
1027	17366350	175	-V------------------------N-----------------------------L---	177
1028	15384987	335	-H------------------------Y-----------------------------IDHF	340
1029	2129898	464	-E------------------------HFNI--------------------------F---	469
1030	2833384	464	-E------------------------HFNI--------------------------F---	469
1031	15028467	392	-H------------------------Y-----------------------------IDF-	397
1032	15643657	198	-E------------------------F-----------------------------Y---	200
983	maize DuI	193	--------------G-------------------K-------------------------	194
984	7489826	1393	--------------G-------------------K-------------------------	1394
985	9502145	1330	--------------G-------------------K-------------------------	1331
986	9502143	1347	--------------G-------------------K-------------------------	1348
987	17646328	935	--------------G-------------------K-------------------------	936
988	4582789	865	--------------G-------------------K-------------------------	866
989	7489274	948	--------------G-------------------R-------------------------	949
990	2833389	948	--------------G-------------------R-------------------------	949
991	15221083	745	--------------G-------------------K-------------------------	746
992	4582783	584	--------------K-------------------G-------------------------	585
993	15717885	610	--------------D-------------------RPDRMRDNSHGRINAVKG--------	628
994	15236819	724	--------------G-------------------SCGLDVNQLNRPDRMQDHSSGDRVNP	750
995	18461221	511	--------------A-------------------P-------------------------	512
996	8901183	350	--------------D-------------------K-------------------------	351
997	17227527	209	--------------K-------------------G-------------------------	210
998	16329217	183	--------------GLNNDSYYFSYDRLQDNFNPN-------------------------	203
999	2811062	202	--------------K-------------------G-------------------------	203
1000	7484399	140	--------------D-------------------K-------------------------	141
1001	18309046	208	--------------K-------------------G-------------------------	209
1002	15903076	202	--------------K-------------------A-------------------------	203
1003	17229371	181	--------------A-------------------A-------------------------	182
1004	15900991	202	--------------K-------------------A-------------------------	203
1005	16124067	175	--------------S-------------------ELHLPAEFFQIYGLEFYGQISYLKA-	200
1006	16080147	181	--------------G-------------------L-------------------------	182
1007	16273270	199	--------------K-------------------S-------------------------	200
1008	15602409	199	--------------K-------------------A-------------------------	200
1009	15672681	181	--------------G-------------------D-------------------------	182
1010	17938870	192	--------------E-------------------R-------------------------	193
1011	16119514	171	--------------E-------------------R-------------------------	172
1012	15613648	196	--------------K-------------------G-------------------------	197
1013	16331219	181	--------------A-------------------A-------------------------	182
1014	17366711	176	--------------G-------------------D-------------------------	177
1015	15805621	213	--------------M-------------------K-------------------------	214
1016	15620537	181	--------------A-------------------A-------------------------	182
1017	15641730	203	--------------SMLR----------------A-------------------------	207
1018	7489711	402	KLYDNIGGDHSNVFA-------------------A-------------------------	417
1019	15605532	197	--------------L-------------------K-------------------------	198
1020	15803938	175	--------------N-------------------DIQLPWSFFNIHGLEFNGQISFLKA-	200
1021	17367076	185	--------------M-------------------K-------------------------	186
1022	16766821	171	--------------A-------------------K-------------------------	172
1023	16762766	171	--------------A-------------------K-------------------------	172
1024	15834801	197	--------------L-------------------K-------------------------	198
1025	15966599	202	--------------K-------------------G-------------------------	203
1026	15618857	196	--------------M-------------------K-------------------------	197
1027	17366350	178	--------------M-------------------KG------------------------	180
1028	15384987	341	RLYDPVGGEHSNVFA-------------------A-------------------------	356
1029	2129898	470	--------------A-------------------A-------------------------	471
1030	2833384	470	--------------A-------------------A-------------------------	471
1031	15028467	398	RLYDPVGGEHSNVFA-------------------A-------------------------	413
1032	15643657	201	--------------G-------------------QLNFLKG-------------------	208
983	maize-Du1	195	---A--------------MR-----------------------------Y----------	198
984	7489826	1395	---A--------------MR-----------------------------Y----------	1398
985	9502145	1332	---A--------------MT-----------------------------Y----------	1335
986	9502143	1349	---A--------------MT-----------------------------Y----------	1352
987	17646328	937	---A--------------MA-----------------------------R----------	940
988	4582789	867	---A--------------MA-----------------------------Y----------	870
989	7489274	950	---A--------------MT-----------------------------N----------	953
990	2833389	950	---A--------------MT-----------------------------N----------	953
991	15221083	747	---A--------------MT-----------------------------F----------	750
992	4582783	586	---A--------------VV-----------------------------Y----------	589
993	15717885	629	---A--------------VV-----------------------------Y----------	632
994	15236819	751	VKGA--------------II-----------------------------F----------	757
995	18461221	513	---D--------------LS-----------------------------Y----------	516
996	8901183	352	---A--------------LDERTIGHNPERLNLMKGGIV----------Y----------	374
997	17227527	211	---G--------------IV-----------------------------Y----------	214
998	16329217	204	---A--------------IN-----------------------------F----------	207
999	2811062	204	---A--------------LV-----------------------------A----------	207
1000	7484399	142	---A--------------LDERTIGHNPERLNLMKGGIV----------Y----------	164
1001	18309046	210	---A--------------IN-----------------------------Y----------	213
1002	15903076	204	---G--------------IL-----------------------------Y----------	207
1003	17229371	183	---A--------------VQ-----------------------------F----------	186
1004	15900991	204	---G--------------IL-----------------------------Y----------	207
1005	16124067	201	---G--------------LF-----------------------------F----------	204
1006	16080147	183	---E--------------MD-----------------------------HFHYERLECNG	196
1007	16273270	201	---G--------------LF-----------------------------Y----------	204
1008	15602409	201	---G--------------LY-----------------------------Y----------	204
1009	15672681	183	---A--------------LSELFGMGMERYFEGVVRHNGMLNMLKTGILY----------	215
1010	17938870	194	---AGMLGLPSHLCTVDCLE-----------------------------Y----------	211
1011	16119514	173	---AGMLGLPSHLCTVDCLE-----------------------------Y----------	190
1012	15613648	198	---A--------------LV-----------------------------H----------	201
1013	16331219	183	---A--------------IQ-----------------------------F----------	186
1013	17366711	178	---A--------------LSELFGMGMERYFEGVVRHNGMLNMLKTGILY----------	210
1014	15805621	215	---AG-------------LN-----------------------------F----------	219
1015	15620537	183	---A--------------LL-----------------------------Y----------	186
1016	15641730	208	---G--------------IA-----------------------------F----------	211
1017	7489711	418	---G--------------LK-----------------------------T----------	421
1018	15605532	199	---G--------------AL-----------------------------Y----------	202
1019	15803938	201	---G--------------LY-----------------------------Y----------	204
1020	17367076	187	---AG-------------LN-----------------------------F----------	191
1021	16766821	173	---H--------------MDDIELPWSFFNMHGLEFNGQLSFLKAGLY-Y----------	204
1022	16762766	173	---H--------------MDDIELPWSFFNMHGLEFNGQLSFLKAGLY-Y----------	204
1023	15834801	199	---G--------------AL-----------------------------Y----------	202
1024	15966599	204	---G--------------LQ-----------------------------T----------	207
1025	15618857	198	---G--------------AL-----------------------------Y----------	201
1026	17366350	181	---G--------------IV-----------------------------F----------	184
1027	15384987	357	---G--------------LK-----------------------------M----------	360
1028	2129898	472	---G--------------LK-----------------------------T----------	475
1029	2833384	472	---G--------------LK-----------------------------T----------	475
1030	15028467	414	---G--------------LK-----------------------------M----------	417
1031	15643657	209	---G--------------IV-----------------------------F----------	212
983	Maizedu1	199	-----------C--------------DK-------------A-----------------T	203
984	7489826	1399	-----------C--------------DK-------------A-----------------T	1403
985	9502145	1336	-----------C--------------DK-------------A-----------------T	1340
986	9502143	1353	-----------C--------------DK-------------A-----------------T	1357
987	17646328	941	-----------C--------------DK-------------A-----------------T	945
988	4582789	871	-----------A--------------DK-------------A-----------------T	875
989	7489274	954	-----------A--------------DK-------------A-----------------T	958
990	2833389	954	-----------A--------------DK-------------A-----------------T	958
991	15221083	751	-----------A--------------DK-------------A-----------------T	755
992	4582783	590	-----------S--------------NI-------------V-----------------T	594
993	15717885	633	-----------S--------------NI-------------V-----------------T	637
994	15236819	758	-----------S--------------NI-------------V-----------------T	762
995	18461221	517	-----------CGLDVEQLDRPDRMQDN-------------AHGRINVAKGGIVYSNIVT	552
996	8901183	375	-----------C--------------NA-------------V-----------------T	379
997	17227527	215	-----------S--------------NA-------------V-----------------T	219
998	16329217	208	-----------M--------------KG-------------G-----------------I	212
999	2811062	208	-----------S--------------DL-------------I-----------------T	212
1000	7484399	165	-----------C--------------NA-------------V-----------------T	169
1001	18309046	214	-----------S--------------DR-------------I-----------------L	218
1002	15903076	208	-----------A--------------NR-------------V-----------------S	212
1003	17229371	187	-----------A--------------DR-------------V-----------------N	191
1004	15900991	208	-----------A--------------NR-------------V-----------------S	212
1005	16124067	205	-----------A--------------DH-------------V-----------------T	209
1006	16080147	197	FVNFMKAGIIAA--------------DH-------------V-----------------T	212
1007	16273270	205	-----------S--------------DA-------------S-----------------T	209
1008	15602409	205	-----------S--------------DA-------------V-----------------T	209
1009	15672681	216	-----------A--------------DR-------------V-----------------N	220
1010	17938870	212	-----------Y--------------DDMSFLKGGLTTASAV-----------------T	229
1011	16119514	191	-----------Y--------------DDMSFLKGGLTTASAV-----------------T	208
1012	15613648	202	-----------S--------------DR-------------V-----------------T	206
1013	16331219	187	-----------A--------------NT-------------V-----------------T	191
1014	17366711	211	-----------A--------------DR-------------V-----------------N	215
1015	15805621	220	-----------A--------------GH-------------V-----------------T	224
1016	15620537	187	-----------A--------------DR-------------V-----------------N	191
1017	15641730	212	-----------A--------------DK-------------V-----------------N	216
1018	7489711	422	-----------A--------------DR-------------V-----------------V	426
1019	15605532	203	-----------CS-------------DF-------------V-----------------T	208
1020	15803938	205	-----------A--------------DH-------------I-----------------T	209
1021	17367076	192	-----------A--------------GH-------------V-----------------T	196
1022	16766821	205	-----------A--------------DH-------------I-----------------T	209
1023	16762766	205	-----------A--------------DH-------------I-----------------T	209
1024	15834801	203	-----------CS-------------DF-------------V-----------------T	208
1025	15966599	208	-----------A--------------SA-------------I-----------------T	212
1026	15618857	202	-----------CS-------------DY-------------I-----------------T	207
1027	17366350	185	-----------A--------------RR-------------V-----------------T	189
1028	15384987	361	-----------A--------------DR-------------A-----------------V	365
1029	2129898	476	-----------A--------------DR-------------I-----------------V	480
1030	2833384	476	-----------A--------------DR-------------I-----------------V	480
1031	15028467	418	-----------A--------------DR-------------A-----------------V	422
1032	15643657	213	-----------S--------------DV-------------I-----------------N	217
983	maize DU1	204	TVSNTYSKE----V-------S--------G-------H-------G-----A-------	218
984	7489826	1404	TVSNTYSKE----V-------S--------G-------H-------G-----A-------	1418
985	9502145	1341	TVSPTYSRD----V-------A--------G-------H-------G-----A-------	1355
986	9502143	1358	TVSPTYSRD----V-------A--------G-------H-------G-----A-------	1372
987	17646328	946	TVSYTYSRE----V-------S--------G-------H-------G-----A-------	960
988	4582789	876	TVSPTYSRE----I-------A--------G-------N-------H-----A-------	890
989	7489274	959	TVSPTYSQE----V-------S--------G-------N-------P-----V-------	973
990	2833389	959	TVSPTYSQE----V-------S--------G-------N-------P-----V-------	973
991	15221083	756	TVSPTYAKE----V-------A--------G-------N-------S-----V-------	770
992	4582783	595	TVSPTYAQE----V-------R--------TAEGGKGLH-------S-----T-------	616
993	15717885	638	TVSPTYALE----V-------R--------S-------E-------G-----GRGLQDT-	658
994	15236819	763	TVSPTYAQE----V-------R--------TAEGGKGLH-------S-----T-------	784
995	18461221	553	TVSPTYALE----V-------R--------S-------E-------G-----GRGLQDT-	573
996	8901183	380	TVSPTYANE----V-------L--------N-------G-------G-----AAGWLRST	401
997	17227527	220	TVSPNHALE----A-------QYTDVGCGLG-------H-------------T-------	241
998	16329217	213	VYSN-YVNT----V-------S--------P-------H-------H-----AWEARFSD	233
999	2811062	213	TVSPTYKEE----I-------Q--------TAYYGERLD-------G-----L-------	234
1000	7484399	170	TVSPTYANE----V-------L--------N-------G-------G-----AAGWLRST	191
1001	18309046	219	TVSETYAKE----I----------------------------------------------	229
1002	15903076	213	TVSPSYAHE----IMT-----S--------Q-------F-------G-----C-------	229
1003	17229371	192	TVSPTYAEQ----I-------KTP------A-------Y-------G-----E-------	208
1004	15900991	213	TVSPSYAHE----IMT-----S--------Q-------F-------G-----C-------	229
1005	16124067	210	TVSPTYAKE----I-------TQPAF----G-------Y-------GMEGLLQ-------	233
1006	16080147	213	TVSPTYRNE----I----------------------------------------------	222
1007	16273270	210	AVSPTYAQE----I-------TTPEFAY--G-------L-------Q-----G-------	230
1008	15602409	210	AVSPTYAKE----I-------TTPEF----G-------Y-------GLQGLLT-------	233
1009	15672681	221	TVSPTYAKE----I-------QTSEFGC--G-------L-------E-----S-------	241
1010	17938870	230	TVSPTYARE----ILTPEMGMG--------M-------H-------G-----V-------	251
1011	16119514	209	TVSPTYARE----ILTPEMGMG--------M-------H-------G-----V-------	230
1012	15613648	207	TVSPTYAQE----I-------Q--------TPAFGEGLH-------G-----L-------	228
1013	16331219	192	TVSPTYAQQ----I-------QTP------A-------Y-------G-----E-------	208
1014	17366711	216	TVSPTYAKE----I-------QTSEFGC--G-------L-------E-----S-------	236
1015	15805621	225	TVSPTYAQE----I-------TTPQY----G-------E-------GLEGLLV-------	248
1016	15620537	192	TVSPTYAQQ----I-------QTP------T-------Y-------G-----E-------	208
1017	15641730	217	AVSPNYAAELLTPL-------G--------A-------H-------G-----L-------	235
1018	7489711	427	TVSNGYMWE----L-------K--------TSEGGWGLH-------D-----I-------	448
1019	15605532	209	TVSPTYAKE----ILEDYSDYE--------I-------H-------D-----A-------	230
1020	15803938	210	AVSPTYARE----I-------TEPQFAY--G-------M-------E-----G-------	230
1021	17367076	197	TVSPTYAQE----I-------TTPQY----G-------E-------GLEGLLV-------	220
1022	16766821	210	AVSPTYARE----I-------TEPQFAY--G-------M-------E-----G-------	230
1023	16762766	210	AVSPTYARE----I-------TEPQFAY--G-------M-------E-----G-------	230
1024	15834801	209	TVSPTYAKE----ILQDYSDYE--------I-------H-------D-----A-------	230
1025	15966599	213	TVSPSYAQE----ILTPEFGMG--------L-------D-------G-----L-------	234
1026	15618857	208	TVSLTYVQE----I-------I--------NDYSDYELH-------D-----A-------	229
1027	17366350	190	TVRPSYAEE----I-------Q--------TPEFGMGLD-------G-----V-------	211
1028	15384987	366	TVSHCYLWE----I-------KTMDGGW--G-------L-------H-----E-------	386
1029	2129898	481	TVSHCYAWE----L-------K--------TSEGGWGLH-------N-----I-------	502
1030	2833384	481	TVSHCYAWE----L-------K--------TSEGGWGLH-------N-----I-------	502
1031	15028467	423	TVSHGYLWE----I-------KTMDGGW--G-------L-------H-----E-------	443
1032	15643657	218	TVSPTYAEE----I-------Q--------T-------EEYGEKLDG-----V-------	239
983	maize Du1	219	--------I---V--PHL-----G-K------------FYGILNGIDPDIWDP------Y	241
984	7489826	1419	--------I---V--PHL-----C-K------------FYGILNGIDPDIWDP------Y	1441
985	9502145	1356	--------I---A--PHR-----E-K------------FYGILNGIDPDIWDP------Y	1378
986	9502143	1373	--------I---A--PHR-----F-K------------FYGILNGIDPDIWDP------Y	1395
987	17646328	961	--------I---A--PHF-----S-K------------FHGIRNGIDPDIWDP------Y	983
988	4582789	891	--------V---A--THL-----H-K------------FHGIINGIDPDIWDP------F	913
989	7489274	974	--------I---A--PHL-----H-K------------FHGIVNGIDPDIWDP------L	996
990	2833389	974	--------I---A--PHL-----H-K------------FHGIVNGIDPDIWDP------L	996
991	15221083	771	--------I---S--AHL-----Y-K------------FHGIINGIDPDIWDP------Y	793
1033	7489827	1	DP------Y	3
992	4582783	617	--------L---S--THS-----K-K------------FIGILNGIDTDIWNP------A	639
993	15717885	659	--------L---K--VHS-----R-K------------FLGILNGIDTDTWNP------C	681
994	15236819	785	--------L---N--FHS-----K-K------------FIGILNGIDTDSWNP------A	807
995	18461221	574	--------L---K--MHS-----R-K------------FVGILNGIDTGTWNP------S	596
996	8901183	402	F-------A---R--PELR----S-K------------FHGILNGIDCEEWNP------A	426
997	17227527	242	--------L---Y--LHK-----E-K------------FSGVLNGIDYDFWNP------E	264
998	16329217	234	ISCGLGHTL---E--IHQ-----Q-K------------FGGILNGLDYEVWNP------E	264
999	2811062	235	--------L---R--ARR-----D-D------------LLGILNGIDDEFYNP------E	257
1000	7484399	192	F-------A---R--PELR----S-K------------FHGILNGIDCEEWNP------A	216
1001	18309046	230	------------T--PYF-----G-ENLDGLLRERGYALKGIVNGIDYDEFNP------S	263
1002	15903076	230	--------N---L--DHILKMESG-K------------VSGIVNGIDADLYNP------Q	257
1003	17229371	209	--------K---I--EGLLSFISG-K------------LSGIVNGIDTEVYDP------A	236
1004	15900991	230	--------N---L--DQILKMESG-K------------VSGIVNGIDADLYNP------Q	257
1005	16124067	234	--------A---L--ARQ-----G-R------------LTGILNGVDSDIWDP------Q	256
1006	16080147	223	--------M---T--PYY-----G-EQLEQVLQYREDDVTGILNGIDDTFYQP------K	257
1007	16273270	231	--------L---L--SGLKAQ--G-R------------LVGILNGVDENIWHP------N	256
1008	15602409	234	--------T---L--NSQ-----G-K------------LVGILNGVDDQIWHP------N	256
1009	15672681	242	--------I---L--QYVD----G-K------------VSGILNGIDYDIYNP------E	265
1010	17938870	252	--------L---A--RRR-----G-D------------LRGIVNGVDHDVWNP------A	274
1011	16119514	231	--------L---A--RRR-----G-D------------LRGIVNGVDHDVWNP------A	253
1012	15613648	229	--------L---H--QER-----G-K------------TRGILNGIDLEDFDP------K	251
1013	16331219	209	--------K---L--EGLLSYLSG-N------------LVGILNGIDTEIYNP------A	236
1014	17366711	237	--------I---L--QYVD----G-K------------VSGILNGIDYDIYNP------E	260
1015	15805621	249	--------R---L--THE-----G-R------------LSGILNGLDQDRWNP------R	271
1016	15620537	209	--------K---L--EGLLSFISG-K------------LSGILNGIDVDSYNP------A	236
1017	15641730	236	--------VDDFV--RRA-----R-D------------LHGIVNGCDYSEWNP------R	261
1018	7489711	449	--------I---N--QND-----W-K------------LQGIVNGIDMSEWNPAVDVHLH	477
1019	15605532	231	--------I---T--ARQ-----H-H------------LRGILNGIDTTIWGP------E	253
1020	15803938	231	--------L---L--QQRHRE--G-R------------LSGVLNGVDEKIWSP------E	256
1021	17367076	221	--------R---L--THE-----C-R------------LSGILNGLDQDRWNP------R	243
1022	16766821	231	--------L---LRQHHLE----G-R------------LSGILNGVDEKIWNF------E	256
1023	16762766	231	--------L---LHQRHLE----G-R------------LSGILNGVDEKIWNP------E	256
1024	15834801	231	--------I---T--ARQ-----H-H------------LKGILNGIDYTILDP------E	253
1025	15966599	235	--------L---S--SRV-----A-D------------LTGIVNGIDGETWDP------Q	257
1026	15618857	230	--------I---L--ARN-----S-V------------FSGIINGIDEDVWNP------K	252
1027	17366350	212	--------L---R--RHA-----G-K------------LRGILNGLDTEVFDP------G	234
1028	15384987	387	--------I---I--NHN-----DWK------------LQGIVNGIDMAEWNP------E	410
1029	2129898	503	--------I---N--ESD-----W-K------------FRGIVNGVDTKDWNP------Q	525
1030	2833384	503	--------I---N--ESD-----W-K------------FHGIVNGVDTKDWNP------Q	525
1031	15028467	444	--------I---I--NHN-----DWK------------LQGIVNGIDMAEWNP------E	467
1032	15643657	240	--------L---R--MHS-----K-D------------LYGILNGIDYELYNP------A	262
983	MaizeDu1	242	NDNFIP----VHYT------CE------NVVEGKHAAKRA-LQQKFGL-Q-QI--D--V-	277
984	7489826	1442	NDNFIP----VHYT------CE------NVVEGKHAAKRA-LQQKFGL-Q-QI--D--V-	1477
985	9502145	1379	TDNFIP----VFYT------CE------NVVEGKPAAKHA-LQQKFGL-Q-QT--D--V-	1414
986	9502143	1396	TDNFIP----VPYT------CE------NVVEGKHAAKRA-LQQKFGL-Q-QT--D--V-	1431
987	17646328	984	SDNFIP----VHYT------SE------NVVEGKSAAKKA-LQQRLGL-Q-QT--D--T-	1019
988	4582789	914	NDNSIP----VPYT------AE------NVVEGKRASKEA-LQQKLGL-K-KA--D--L-	949
989	7489274	997	NDKFIP----IPYT------SE------NVVEGKTAAKEA-LQHKLGL-K-QA--D--L-	1032
990	2833389	997	NDKFIP----IPYT------SE------NVVEGKTAAKEA-LQHKLGL-K-QA--D--L-	1032
991	15221083	794	NDNFIF----VPYT------SE------NVVEGKHAAKEE-LQNHLGL-K-SA--D--F-	829
1033	7489827	4	NDNFIP----VHYT------CE------NVVEGKHAAKRA-LQQKFGL-Q-QI--D--V-	39
992	4582783	640	TDPFLQ----VQYN------A-------NDLQGKSENKEA-LRRNLGL-S-SA--D--VR	675
993	15717885	682	TDRYLK----VQYN------AK------D-LQGKAANKAA-LREQLNL-A-SAYPS--Q-	718
994	15236819	808	TDPFLK----AQFN------AK------D-LQGKEENKMA-LRKQLGL-s-SA--E--SR	843
995	18461221	597	TDRFLA----VQYS------AT------D-LQGKAANKAF-LRKQLGL-Y-SE--D--AS	632
996	8901183	427	TDALLP----ANFD------AD------RPA-GKALCKEF-LQKGLGL---EV--DPRK-	462
997	17227527	265	IDRHIP----DNYS-------Q------DDFEQKLYNKKA-LRERLLL-Q-AA--D--K-	299
998	16329217	265	IDPLLA----SNFS------VK------TFGD-KAKNKQA-LRERLLL-E-TD--DK-K-	300
999	2811062	258	ADPFLT----ATYS------V-------HTRERKQLNKRA-LQRQFGL-P-EWD-D--V-	293
1000	7484399	217	TDALLP----ANFD------AD------RPA-GKALCKEF-LQKGLGL---EV--DPRK-	252
1001	18309046	264	KDSLIA----KNFS------VK-------TIEDKVLNKLA-LQKELGL-PINP--D--I-	299
1002	15903076	258	TDALLD----YHFN------QE------D-LSGKAKNKAK-LQERVGL-PVRA--D--V-	293
1003	17229371	237	NDKYIA----QTFT------AD-------TLDKRKANKIA-LQEEVGL-EVNS--N--A-	272
1004	15900991	258	TDALLD----YHFN------QE------D-LSGKAKNKAK-LQERVGL-PVRA--D--V-	293
1005	16124067	257	SDTLLP----TRYD------AE------N-LQAKAINKTH-LQTAMGL-Q-LA--EN-K-	292
1006	16080147	258	SDPYIE----AQYD------SG------DLA-CKLENKTK-LQQRMGLPE-KN--D--I-	293
1007	16273270	257	VDQYIP----HHYK------LK-------YMAGKKKNKAE-LQAYFNL-P-QD--E--S-	291
1008	15602409	257	HDAYIE----HEYK------LK-------AMTGKRKNKEA-LQAYFNLPQ-DP--D--A-	292
1009	15672681	266	NDILIP----YHFS-------E------EELSGKGQMKAE-LQKRTGL-PLNP--N--V-	301
1010	17938870	275	TDPYIL----ANFT------AA------TATR-RSLNKYA-LLQALGL-A-PT--Q--G-	309
1011	16119514	254	TDPYIL----ANFT------AA------TATR-RSLNKYA-LLQALGL-A-PT--Q--G-	288
1012	15613648	252	TDPHVT----YPYK-------H------NQME-KRKNKQV-IQRLFELPE-RK--D--I-	286
1013	16331219	237	EDRFIS----NVFD------AD------S-LDKRVKNKIA-IQEETGL-EINR--N--A-	272
1014	17366711	261	NDILIP----YHFS-------E------EELSGKGQMKAE-LQKRTGL-PLNP--N--V-	296
1015	15805621	272	TDPDIA----AY----------------SDPAGKAGAVKA-LRQEFGL-D-----D--A-	301
1016	15620537	237	TDRGXV----ANYD------RD------TLI-ARLNNRLA-LQKEMGL-EVNP--D--R-	272
1017	15641730	262	TDHYLP----ATYSDE----PE------SMRKGKALCKTA-LQEELHL-P-VT--D--V-	299
1018	7489711	478	SDDY------TNYT------FE------TLDTGKRQCKAA-LQRQLGL-QVRD--D--V-	512
1019	15605532	254	TDPNLA----KNYT------KELFETPSIFFEAKAENKKA-LYERLGL-S-LE--H--S-	295
1020	15803938	257	TDLLLA----SRYT-------R------DTLEDKAENKRQ-LQIAMGL-K-VD--DK-V-	292
1021	17367076	244	TDPDIA----AY----------------SDPAGKAGAVKA-LRQEFGL-D-----D--A-	273
1022	16766821	257	SDLLLA----SRYT-------R------DTLEEKAENKRQ-LQIAMGL-K-VN--DK-V-	292
1023	16762766	257	SDLLLA----SRYT-------R------DTLEEKAENKRQ-LQIAMGL-K-VN--DK-V-	292
1024	15834801	254	TDPHLA----KNYSKVLFEDPK------AFFEAKAENKKA-LYETLGL-S-LD--K--S-	295
1025	15966599	258	TDPHIP----AHYG-------P------GTLKRRAGNRKA-LEERFGL-E-KG--P--G-	292
1026	15618857	253	TDPALA----VQYD------ASLLSEP-DVLFTKKEENRAVLYEKLGI-S-SD--Y--F-	294
1027	17366350	235	KDPYLP----APYT------RE------D-PSGKARAKEV-FRERTGL-R-P--------	266
1028	15384987	411	VDEHLQSDGYANYT------FE------TLDTGKKQCKEA-LQRQLGL-QVRD--D--V-	451
1029	2129898	526	FDAYLTSDGYTNYN------LK------TLQTGKRQCKAA-LQRELGL-PVRE--D--V-	566
1030	2833384	526	FDAYLTSDGYTNYN------LK------TLQTGKRQCKAA-LQRELGL-PVRE--D--V-	566
1031	15028467	468	VDEHLQSDGYANYT------FE------TLDTGKKQCKEA-LQRQLGL-QVRD--D--V-	508
1032	15643657	263	TDRYIY----VNYD------V-------NRLELKWENKVK-LQEELGL-PVNK--E--T-	298

[0409]

TABLE XXXIV
Maize soluble starch synthase III (Du I)
“GLYTR” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id.No.	Number	#	(start)                     Sequence                     end	#
1034	MaizeDu1	278	-PVVGI-VTRLTAQKGIHLIKH-AIHRTLE-RNG-QVVLLGS-A-PDSRIQADFVNLANT	330
1035	7489826	1478	-PVVGI-VTRLTAQKGIHLIKH-AIHRTLE-RNG-QVVLLGS-A-PDSRIQADFVNLANT	1530
1036	9502145	1415	-PIVGI-ITRLTAQKGIHLIKH-AIHRTLE-SNG-QVVLLGS-A-PDHRIQGDFCRLADA	1467
1037	9502143	1432	-PIVGI-ITRLTAQKGIHLIKH-AIHRTLE-SNG-HVVLLGS-A-PDHRIQGDFCRLADA	1484
1038	17646328	1020	-PVVGI-ISRLTVQKGIHLIKH-AIYRTLE-RNG-QVVLLGS-A-PDHRIQGDFTNLASK	1072
1039	4582789	950	-PLVGV-ITRLTHQKGIHLIKH-AIWRTLE-RGG-QVVLLGS-A-PDHRIQNDFVNLANQ	1002
1040	7489274	1033	-PLVGI-ITRLTHQKGIHLIKH-AIWRTLE-RNG-QVVLLGS-A-PDPRVQNDFVNLANQ	1085
1041	2833389	1033	-PLVGI-ITRLTHQKGIHLIKH-AIWRTLE-RNG-QVVLLGS-A-PDPRVQNNFVNLANQ	1085
1042	15221083	830	-PVVGI-ITRLTHQKGIHLIKH-AIWRTLE-RNG-QVVLLGS-A-PDPRIQNDFVNLANQ	882
1043	7489827	40	-PVVGI-VTRLTAQKGIHLIKH-AIHRTLE-RNG-QVVLLGS-A-PDARIQADFVNLANK	92
1044	4582783	676	RPLVGC-ITRLVPQKGVHLIRH-AIYLTLE-LGG-QFVLLGS-S-PVPHIQREFEGIAN-	728
1045	15717885	719	-PLVGC-ITRLVAQKGVHLIRH-AIYKTAE-LGG-QFVLLGS-S-PVPEIQREFEGIAD-	770
1046	15236819	844	RPLVGC-ITRLVPQKGVHLIRH-AIYRTLE-LGG-QFVLLGS-S-PVPHIQREFEGIEQQ	897
1047	18461221	633	QPLVAC-ITRLVPQKGLHLIRH-AIYKTAE-LGG-QFVLLGS-S-PVPHIQREFEGVADQ	686
1048	8901183	463	-PLVAV-VSRLVPQKGIHLIKA-ALFRTVE-KGG-QFVLLGS-G-HSD---PAFRQLADQ	512
1049	17227527	300	-PIIAY-IGRLDNQKGVHLVHH-AIYHALN-KGA-QFVLLGS-A-TEAGINAHFRHEKQF	352
1050	16329217	301	-PMLCF-IGRLDGQKGVHLVHH-SIYYALS-QGA-QFVLLGS-A-TEPNLSKWFWHEKQH	353
1051	2811062	294	-PLIAM-VTRMTAQKGLDLVTC-VFHEMMS-EDM-QLVVLGT-G--DWRFEQFFSQMA--	343
1052	7484399	253	-PLVAV-VSRLVPQKGIHLIKA-ALFRTVE-KGG-QFVLLGS-G-HSD---PAFRQLADG	302
1053	18309046	300	-PMISI-VSRLTNQKGCDLIVN-IANRLLQ-RNV-QLVILGT-G------DYNYENHFKG	347
1054	15903076	294	-PLVGI-VSRLTRQKGFDVVVE-SLHHILQ-EDV-QIVLLGT-G--DPAFEGAFSWFAQ-	344
1055	17229371	273	-FLIGM-VTRLVEQKGLDLVIQ-MLDRFMAYTDA-QFVLLGT-G--DRYYETQMWQLASR	325
1056	15900991	294	-PLVGI-VSRLTRQKGFDVVVE-SLHHILQ-EDV-QIVLLGT-G--DPAFEGAFSWFAQ-	344
1057	16124067	293	-PIFAV-VSRLTVQKGLDLVLE-ALPELLA-LGG-QLVVLGS-G--DATLQEAFLAAA--	342
1058	16080147	294	-PLISM-VTRLTKQKGLDLVRR-IMHELLE-EQDIQLVVLGT-G------EREFEDYFRY	342
1059	16273270	292	-ALAFVMVTRLTEQKGVDLLIE-SADEIVK-QGG-QLMILGSGA-P--HLEQGIRELAER	344
1060	15602409	293	-LLFVM-VTRLTEQKGVDLLID-SAEEIVK-QGG-QLTILGS-G--SPHLEAGILHLAQQ	344
1061	15672681	302	-PLIGM-VSRLTNQKGFDLVLS-QLEKVLE-ENV-QIVLLGT-GFPE--IEEGFRYFSQK	353
1062	17938870	310	-PVFGV-VSRLTWQKGIDLLPH-VVPLIIE-RKG-RLIVHGE-G--DTALEDSLQALAKR	361
1063	16119514	289	-PVFGV-VSRLTWQKGIDLLPH-VVPLIIE-RKG-RLIVHGE-G--DTALEDSLQALAKR	340
1064	15613648	287	-PLIAM-VSRLVEEKGVPLLTQIAGELVTT-ENV-QLAILGT-G--DPSLEDQLHHLA-S	338
1065	16331219	273	-MVVGI-VARLVEQKGIDLVIQ-ILDRFMSYTDS-QLIILGT-G--DRHYETQLWQMASR	325
1066	17366711	297	-PLIGM-VSRLTNQKGFDLVLS-QLEKVLE-ENV-QIVLLGT-GFPE--IEEGFRYFSQK	348
1067	15805621	302	-PILAT-VSRLADQKGMDLLIT-ALPE-LV-RDW-NVVVLGG-G--DPLLEAALTGWA--	350
1068	15620537	273	-FLIGF-VARLVEQKGIDLLLQ-ILDRFLSYSDA-QFVVLGT-G--ERYYETQLWELATR	325
1069	15641730	300	-PLFGM-VCRLTHQKGFHYLLP-ILEQFLR-NNV-QVVIVGT-G------EPEVAARLNK	347
1070	7489711	513	-PLIGF-IGRLDHQKGVDIIAD-AIHWIAG-QDV-QLVMLGT-G------RADLEDMLRR	560
1071	15605532	296	-PCVCI-ISRIAEQKGPHFMKQ-AILHALE-NAY-TLIIIGT-C-YGNQLHEEFANLQES	348
1072	15803938	293	-PLFAV-VSRLTSQKGLDLVLE-ALPGLLE-QGG-GLALLG--A-GDPVLQEGFLAAAAE	344
1073	17367076	274	-PILAT-VSRLADQKGMDLLIT-ALPE-LV-RDW-NVVVLGG-G--DPLLEAALTGWA--	322
1074	16766821	293	-PLFAV-VSRLTNQKGLDLVLE-ALPGLLE-QGG-QLALLG--A-GDPVLQEGFLAAA--	342
1075	16762766	293	-PLFAV-VSRLTNQKGLDLVLE-ALPGLLE-QGG-QLALLG--A-GDPVLQEGFLAAA--	342
1076	15834801	296	-PCMCI-ISRIAEQKGPEFMKQ-AILHALE-NAY-TLIIIGT-C-YGGQIHKEFSNLQES	348
1077	15966599	293	-PIFCV-ISRLTWQKGMDLVAE-AADDIVA-LGG-KLVVLGS-G--DPALESALMAAASR	344
1078	15618857	295	-PLICV-ISRIVEEKGPEFMKE-IILHAME-HSY-AFILIGT-S-QNEVLLNEFRNLQDC	347
1079	17366350	267	-PVLAY-VGRLDYQKGLDLVLK-ALPRLLE-MGF-RLYVQGV-G--DGGLQEAFLRAEEE	318
1080	15384987	452	-PLIGF-IGRLDHQKGVDIIGD-AMPWIAG-QDV-QVVMLGT-G------RPDLEEMLRR	499
1081	2129898	567	-PIISF-IGRLDHQKGVDLIAE-AIPWMMS-HDV-QLVMLGT-G------RADLEQMLKE	614
1082	2833384	567	-PIISF-IGRLDHQKGVDLIAE-AIPWMMS-HDV-QLVMLGT-G------RADLEQMLKE	614
1083	15028467	509	-PLIGF-IGRLDHQKGVDIIGD-AMPWIAG-QDV-QVVMLGT-G------RPDLEEMLRR	556
1084	15643657	299	-AVAGL-ISRLVPQKGLDLLVD-VMDYLTL-FDL-QIVVLGT-G-DEQ-----YENAFRK	346
1034	Maize DU1	331	LHGVNHGQVRLSLTYDEPLSHLIYAGSDFILVPSIFEPCGLTQLVAMRYGTIPIVRKTGG	390
1035	7489826	1531	LHGVNHGQVRLSLTYDEPLSHLIYAGSDFILVPSIFEPCGLTQLVAMRYGTIPIVRKTGG	1590
1036	9502145	1468	LHGVYHGRVKLVLTYDEPLSHLIYAGSDFIIVPSIFEPCGLTQLVAMRYGSIPIVRKTGG	1527
1037	9502143	1485	LHGVYHGRVKLVLTYDEPLSHLIYAGSDFIIVPSIFEPCGLTQLVAMRYGSIPIVRKTGG	1544
1038	17646328	1073	LHGEYHGRVKLCLTYDEPLSHLIYAGADFILVPSMFEPCGLTQLTAMRYGSIPIVRKTGG	1132
1039	4582789	1003	LHSSHNDRARLCLAYDEPLSHMIYAGADFILVPSIFEPCGLTQLTAMRYGSIPIVRKTGG	1062
1040	7489274	1086	LHSKYNDRARLCLTYDEPLSHLIYAGADFILVPSIFEPCGLTQLTAMRYGSIPVVRKTGG	1145
1041	2833389	1086	LHSKYNDRARLCLTYDEPLSHLIYAGADFILVPSIFEPCGLTQLTAMRYGSIPVVRKTGG	1145
1042	15221083	883	LHSSHGDRARLVLTYDEPLSHLIYAGADFILVPSIFEPCGLTQLIAMRYGAVPVVRKTGG	942
1043	7489827	93	LHGVNHGQCRLSLTYDEPLSHLIYAGSDFILVPSIFEPCGLTHLVAMRYGTIPIVRKTGG	152
1044	4582783	729	-HFQNHDHIRLILKYDESLSHAIYAASDMFIIPSIFEPCGLTQMISMRYGAIPIARKTGG	787
1045	15717885	771	-HFQNNNNIRLILKYDDALSHCIYAASDMFIVPSIFEPCGLTQMIAMRYGSVPIVRKTGG	829
1046	15236819	898	FK--SHDHVRLLLKYDEALSHTIYAASDLFIIPSIFEPCGLTQMIAMRYGSIPIARKTGG	955
1047	18461221	687	FQKNNN--IRLILKYDEALSHCIYAASDMFIIPSMFEPCGLTQMIAMRYGSVPIVRQTGG	744
1048	8901183	513	QFK-DHPNCRLKIMYSERLAHMIYAAADVVVVPSMFEPCGLTQMIALRYGAVPLVRRTGG	571
1049	17227527	353	LN--SNPDVHLELGFNEELSHLIYAGADMIVVPSNYEPCGLTQMIGLKYGTVPIVRGVGG	410
1050	16329217	354	LN--DNPNVHLELGFDEELAHLIYGAADIIVVPSNYEPCGLTQMIGLRYGAVPVVRGVGG	411
1051	2811062	344	--AAYPGKVGVYIGFHEPLAHQIYAGADLFLIPSLFEPCGLSQMIALRYGTIPIVRETGG	401
1052	7484399	303	QFK-DHPNCRLKIMYSERLAHMIYAAADVVVVPSMFEPCGLTQMIALRYGAVPLVRRTGG	361
1053	18309046	348	LQELYPTKVSANIKFDNGLAHRIYASSDIFLMPSLFEPCGLGQLIALRYGAIPIVRETGG	407
1054	15903076	345	---IYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGG	401
1055	17229371	326	Y----PGRMATYLLYNDALSRRIYAGTDAFLMPSRFEPCGISQMMALRYGSIPIVRRTGG	381
1056	15900991	345	---IYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGG	401
1057	16124067	343	--AEHSGQVGVQIGYHEAFSHRIIAGSDVILVPSRFEPCGLTQLYGLKYGTLPLVRHTGG	400
1058	16080147	343	AEFAFHEKCRAYIGFDEPLAHQIYAGSDMFLMPSKFEPCGLGQLIALQYGAIPIVRETGG	402
1059	16273270	345	Y----PQNIAVKIGYDEALSHLMVAGGDVILVPSRFEPCGLTQLYGLQYGTLPLVRKTGG	400
1060	15602409	345	Y----PHQIAVKIGYDEALSHLMIAGGDVILVPSRFEPCGLTQLYGLKYGTLPLVRATGG	400
1061	15672681	354	Y----PDKLSANIAFDIQFAQEIYAGSDFFLMPSAFEPCGLSQMIAMRYGTLPIVHEIGG	409
1062	17938870	362	YPEL----VCAHIGYDERLAHMIQAGSDFIIQPSRFEPCGLTQLYLARYGALPIVSRTGG	417
1063	16119514	341	YPEL----VCAHIGYDERLAHMIQAGSDFIIQPSRFEPCGLTQLYALRYGALPIVSRTGG	396
1064	15613648	339	LHP---HQISFKCVFAEPLARKLYAGADLFIMPSRFEPCGLSQMISLRYETVPIVRETGG	395
1065	16331219	326	F----PGRMAVQLLHNDALSRRVYAGADVFLMPSRFEPCGLSQLMAMRYGCIPIVRRTGG	381
1066	17366711	349	Y----PDKLSANIAFDIQFAQEIYAGSDFFLMPSAFEPCGLSQMIAMRYGTLPIVHEIGG	404
1067	15805621	351	----NHPRVAFASGMNEPLAHRIYAGAHAFAMPSRFEPCGLSQLIAMRYGTLPIVRETGG	406
1068	15620537	326	Y----PGRMSTYLMYDEGLSRRIYAGSDAFLVPSRFEPCGITQMLALRYGSVPIVRRTGG	381
1069	15641730	348	IAHYHRAKFAFVETYSERLAHWVEAGSDFFLMPSEFEACGLNQIYSMAYGTLPIVREVGG	407
1070	7489711	561	FESEHSDKVRAWVGFSVPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	620
1071	15605532	349	L--ANSPDVRILLTYSDVLARQIFAAADMICIPSMFEPCGLTQMIGMRYGTVPLVRATGG	406
1072	15803938	345	Y----PGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTLPLVRRTGG	400
1073	17367076	323	----NHPRVAFASGMNEPLAHRIYAGAHAFAMPSRFEPCGLSQLIAMRYGTLPIVRETGG	378
1074	16766821	343	--AEHPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTLPLVRRTGG	400
1075	16762766	343	--AEHPGQAGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTLPLVRRTGG	400
1076	15834801	349	L--ADSPNVRILLTYSDVLARQIFAAADMICIPSMFEPCGLTQMIGMRYGTVPVVRATGG	406
1077	15966599	345	----NRGHIGMVTGYDEPLSHLMQAGADAILIPSRFEPCGLTQLYGLRYGCVPVVARTGG	400
1078	15618857	348	L--ASSPNIRLILDFNDPLARLTYAAADMICIPSHREACGLTQLIAMRYGTVPLVRKTGG	405
1079	17366350	319	----NPEGVRFLPAYDEAMARLAYAGAEAVLVPSRFEPCGLVQMIASRYGTPPVARAVGG	374
1080	15384987	500	FESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYGTVPVVHAVGG	559
1081	2129898	615	FEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVGG	674
1082	2833384	615	FEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVGG	674
1083	15028467	557	FESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYSTVPVVHAVGG	616
1084	15643657	347	FQERYPDKVSANIKFDVELAQKIYAGADIFLMPSRYEPCGLGQMFSMRYGTIPVVRYTGG	406
1034	MaizeDu1	391	LFDTV--FD--VDNDKERARDRGLE---P-----N-GFS-FDGA-DS-------------	422
1035	7489826	1591	LFDTV--FD--VDNDKERARDRGLE---P-----N-GFS-FDGA-DS-------------	1622
1036	9502145	1528	LYDTV--FD--VDNDKDRARSLGLE---P-----N-GFS-FDGA-DS-------------	1559
1037	9502143	1545	LHDTV--FD--VDNDKDRARSLGLE---P-----N-GFS-FDGA-DS-------------	1576
1038	17646328	1133	LYDTV--FD--VDDDKDRAREQGLE---P-----N-GFS-FEGA-DS-------------	1164
1039	4582789	1063	LYDTV--FD--VDNDKDRAQVQGLE---P-----N-GFS-FDGA-DA-------------	1094
1040	7489274	1146	LYDTV--FD--VDHDKERAQQCGLE---P-----N-GFS-FDGA-DA-------------	1177
1041	2833389	1146	LYDTV--FD--VDHDKERAQQCGLE---P-----N-GFS-FDGA-DA-------------	1177
1042	15221083	943	LFDTV--FD--VDHDKERAQAQVLE---P-----N-GFS-FDGA-DA-------------	974
1043	7489827	153	LFDTV--FD--VDNDKERARDRGLE---P-----N-GFS-FDGA-DS-------------	184
1044	4582783	788	LNDSV--FD--VDDDTIPSQFR------------N-GFT-FLNA-DE-------------	815
1045	15717885	830	LNDSV--FD--FDDETIPMEVR------------N-GFT-FVKA-DE-------------	857
1046	15236819	956	LNDSV--FD--IDDDTIPTQFQ------------N-GFT-FQTA-DEQLKIGMEIYLVWF	996
1047	18461221	745	LCDSV--FD--FDDETIPVELR------------N-GFT-FART-DE-------------	772
1048	8901183	572	LADTV--FD--VDGP---AGGPAQP---R-----N-GFV-FDGS-DD-------------	600
1049	17227527	411	LVNTV--FD--RDYDQNLPPEK------R-----N-GYV-FYQS-DN-------------	439
1050	16329217	412	LVNTV--FD--RDYDQNHPPEK------R-----N-GFV-FYQP-DE-------------	440
1051	2811062	402	LNDTV-----------QSYNEITKE---G-----N-GFS-FTNF-NA-------------	426
1052	7484399	362	LADTV--FD--VDGP---AGGPAQP---R-----N-GFV-FDGS-DD-------------	390
1053	18309046	408	LKDTI--HS--YN------KYTGI----G-----N-GFS-FTNY-NH-------------	432
1054	15903076	402	LRDTV----------------RAFN---PIEGSGT-GFS-FDNL-SP-------------	426
1055	17229371	382	LVDTV--SH--HDPINEAG---------------T-GYC-FDRY-EP-------------	406
1056	15900991	402	LRDTV----------------RAFN---PIEGSGT-GFS-FDNL-SP-------------	426
1057	16124067	401	LADTV------VDCALENLADG--S---A-----S-GFV-FNEC-EA-------------	428
1058	16080147	403	LYDTV--RA--YQEEEGTG---------------N-GFT-FSAF-NA-------------	427
1059	16273270	401	LADTV--VD--STSESIKAR----T---A-----T-GFV-FESA-TP-------------	428
1060	15602409	401	LADTV--VNATVENIKSRL---------A-----T-GFV-FEQA-NR-------------	428
1061	15672681	410	LKDTV--IP--FNPISK-------E---G-----T-GFG-FVDF-EG-------------	434
1062	17938870	418	LAETI--ID---------ANDAAIEAGVA-----T-GFQ-FEPA-NE-------------	445
1063	16119514	397	LAETI--ID---------ANDAAIEAGVA-----T-GFQ-FEPA-NE-------------	424
1064	15613648	396	LYDTI--QS--YNEEIG-------E---G-----N-GFS-FTHY-NA-------------	420
1065	16331219	382	LVDTV--SF--YDPINEAG---------------T-GYC-FDRY-EP-------------	406
1066	17366711	405	LKDTV--IP--FNPISK-------E---G-----T-GFG-FVDF-EG-------------	429
1067	15805621	407	LADTV-------------------P---P-----EVGFR-FADA-TA-------------	424
1068	15620537	382	LVDTV--FH--HD-------PRHAE---G-----N-GYC-FDRY-EP-------------	406
1069	15641730	408	LKDTV--ND--YDKFPERA---------------T-GFG-YQEP-TP-------------	432
1070	7489711	621	LRDTVAPFD--PFNDT------GL------------GWT-FDRA-EA-------------	645
1071	15605532	407	LADTV--AN-------------GI----------N-GFSFFNPH-DF-------------	426
1072	15803938	401	LADTV--SD--C--SLENLAD-GV----A-----S-GFV-FEDS-NA-------------	428
1073	17367076	379	LADTV-------------------P---P-----EVGFR-FADA-TA-------------	396
1074	16766821	401	LADTV------SDSSLENLAD-GI----A-----S-GFV-FEDS-NA-------------	428
1075	16762766	401	LADTV------SDSSLENLAD-GI----A-----S-GFV-FEDS-NA-------------	428
1076	15824801	407	LADTV--TD-------------GV----------N-GFS-FSNPHDF-------------	426
1077	15966599	401	LTDTI--ID--ANEAALSAKC-------A-----T-GFH-FLPV-TT-------------	428
1078	15618857	406	LADTV--IP-------------GV----------N-GFTFFDTN-NF-------------	425
1079	17366350	375	LKDTV--ED-------GRA-----------------GVL-FETY-HP-------------	393
1080	15384987	560	LRDTV--AP--FDP----FADTGL------------GWT-FDRA-EA-------------	584
1081	2129898	675	LRDTV--QP--FNPFDESGV----------------GWT-FDRA-EA-------------	699
1082	2833384	675	LRDTV--QP--FNPFDESGV----------------GWT-FDRA-EA-------------	699
1083	15028467	617	LRDTV--AP--FDP----FADTGL------------GWT-FDRA-EA-------------	641
1084	15643657	407	LADTV--KE--YD-------PQSME---G-----T-GFG-FKKY-DS-------------	431

[0410]

TABLE XXXV
Maize soluble starch synthase III (Du I)
“CTEND” Domain Alignments with other similar proteins
SEQ	Accession	a.a		a.a.
Id. No.	Number	#	(start) Sequence end	#
1085	MaizeDu1	423	---------------NGVDYALNRAISAW--F-DARSWFHSLCKRVMEQDWSWNRPALDY	464
1086	7489826	1623	---------------NGVDYALNRAISAW--F-DARSWFHSLCKRVMEQDWSWNRPALDY	1664
1087	9502145	1560	---------------NGVDYALNRAIGAW--F-DARSWFHSLCKRVMEQDWSWNRPALDY	1601
1088	9502143	1577	---------------NGVDYALNRAIGAW--F-DARSWFHSLCKRVMEQDWSWNRPALDY	1618
1089	17646328	1065	---------------NGVDYALDRAITTW--Y-DARSWFHSLCKRVMEQDWTWNRPALDY	1206
1090	4582789	1095	---------------GGVDYALNRAISAW--Y-DGREWFNTLCKTVMEQDWSWNRPALDY	1136
1091	7489274	1178	---------------GGVDYALNRALSAW--Y-DGRDWFNSLCKQVMEQDWSWNRPALDY	1219
1092	2833389	1178	---------------GGVDYALNRALSAW--Y-DGRDWFNSLCKQVMEQDWSWNRPALDY	1219
1093	15221083	975	---------------PGVDYALNRAISAW--Y-DGREWFNSLCKTVMEQDWSWNRPALEY	1016
1094	7489827	185	---------------NGVDYALNRAISAW--F-DARSWFHSLCKRVMEQDWSWNRPALDY	226
1095	4582783	816	---------------KGINDALVRAINLF--TNDPKSW-KQLVQKDMNIDFSWDSSAAQY	857
1096	15717885	858	---------------QGLSSAMERAFNCY--T-RKPEVWKQLVQKDMTIDFSWDTSASQY	899
1097	15236819	997	SFTCPSLAEKGNVKKQGFNYALERAFNHY--K-KDEEKWMRLVEKVMSIDFSWGSSATQY	1053
1098	18461221	773	---------------QDLSSCLERAFSYY--S-RKPMVWKQLVQKDMQIDFSWDSPASQY	814
1099	8901183	601	---------------GALHGALDRALTLY--T-TQPERWAALQQDNMRLDVSWGKSAKSY	642
1100	17227527	440	---------------QALESAMNRAIDLW--Y-QSPEQFQQLAIQGMKYDYSWNNPGTEY	481
1101	16329217	441	---------------YALETALSRAIALY--K-DDPVAFKTLALQGMAYDYSWNKPGLQY	482
1102	2811062	427	---------------HDMLYTIRRALSFY--R-QPSVW-EQLTERAMRGDYSWRRSANQY	467
1103	7484399	391	---------------GALHGALDRALTLY--T-TQPERWAALQQDNMRLDVSWGKSAKSY	432
1104	18309046	433	---------------NDLMHVIELALETY--D-DKEIW-RSLIIQAMDSDNSWNKSAEKY	473
1105	15903076	427	---------------YWLNWTFQTALDLY--R-NHPDIWRNLQKQAMESDFSWDTACKSY	468
1106	17229371	407	---------------LDLFTCM---IRAWEGF-RYKPQWQELQKRGMSQDFSWYKSAKEY	447
1107	15900991	427	---------------YWLNWTFQTALDLY--R-NHPDIWRNLQKQAMESDFSWDTACKSY	468
1108	16124067	429	---------------QALVKAIRRAFVLW--S-RPKHWRH-VQRHAMRLDFGWQLAAVDY	469
1109	16080147	428	---------------HDLKFTIERALSFY--C-QQDVW-KSIVKTAMNADYSWGKSAKEY	468
1110	16273270	429	---------------EALRHCLQRAFALW--Q-KPRAW-AMVRTDAMEQDFSWRKAAEQY	469
1111	15602409	429	---------------EALRQALVNAFALW--Q-KQRLWF-TVRSVAMEQDFSWQISATGY	469
1112	15672681	435	---------------QILVETINRALEVY--G-KEPEVLNKMVLSAMSKDFSWGTKAQQY	476
1113	17938870	446	---------------DDLRAALERAISAY--N-D-RELFRRLQTQAMQANFSWDKSAAQY	486
1114	16119514	425	---------------DDLRAALERAISAY--N-D-RELFRRLQTQAMQANFSWDKSAAQY	465
1115	15613648	421	---------------HDFLYTIKRALRFY--R-TEKEW-ENLLLNIYSSEVGWDVSAKQY	461
1116	16331219	407	---------------LDCFTAMVRAWEGF--R-FKADW-QKLQQRAMRADFSWYRSAGEY	447
1117	17366711	430	---------------QILVETINRALEVY--G-KEPEVLNKMVLSAMSKDFSWGTKAQQY	471
1118	15805621	425	---------------PAFLQACREAQAAF--Q-DPAQW-QTRATRAMSLDFSWDGPARQY	465
1119	15620537	407	---------------LDLYTCLVRAWESY--Q-YQPQW-QKLQQRGMAVDLSWKQSGIAY	447
1120	15641730	433	---------------EALLITMQRALLFY--L-QQPEEMLKVQQRAMQQNFSWEESAQEY	474
1121	7489711	646	---------------NRMIDALSHCLTTY--R-NYKESWRACRARGMAEDLSWDHAAVLY	687
1122	15605532	427	---------------YEFRNMLSEAVTTY--R-TNHDKWQHIVRACLDFSSDLETAANKY	468
1123	15803938	429	---------------WSLLRAIRRAFVLW--S-RPSLW-RFVQRQAMAMDFSWQVAAKSY	469
1124	17367076	397	---------------PAFLQACREAQAAF--Q-DPAQW-QTRATRAMSLDFSWDGPARQY	437
1125	16766821	429	---------------WSLLRAIRRAFVLW--S-RPSLW-RFVQRQAMAMDFSWQVAAKSY	469
1126	16762766	429	---------------WSLLRAIRRAFVLW--S-RPSLW-RFVQRQAMAMDFSWQVAAKSY	469
1127	15834801	427	---------------HEFRNMLSKAIATY--R-DDQDKWQQIVRSCLEFSSDLETAANKY	468
1128	15966599	429	---------------DGLRLAIRRVLRAY--N-EPKLW-ARLQYQGMKSDVSWAKSAERY	469
1129	15618857	426	---------------NEFRAMLSNAVTTY--R-QEPDVWLNLIESGMLRASGLDAMAKHY	467
1130	17366350	394	---------------EGLLYGVLRLF---------RLGAEEMGLRAMEKDFSWEGPARAY	429
1131	15384987	585	---------------NRMIDALGHCLNTY--R-NYKESWRGLQARGMAQDLSWDHAA---	623
1132	2129898	700	---------------NKLMAALWNCLLTY--K-DYKKSWEGIQERGMSQDLSWDNAAQQY	741
1133	2833384	700	---------------NKLMAALWNCLLTY--K-DYKKSWEGIQERGMSQDLSWDNAAQQY	741
1134	15028467	642	---------------NRMIDALGHCLNTY--R-NYKESWRGLQARGMAQDLSWDHAA---	680
1135	15643657	432	---------------AHLLKAVSKALHFY--Y-REKDHWRRIMTNAMNTDLSWDRSAKEY	473
1085	MaizeDu1	465	IELY-RSASKL	474
1086	7489826	1665	IELY-RSASKL	1674
1087	9502145	1602	IELY-HAARK	1610
1088	9502143	1619	IELY-HAARK	1627
1089	17646328	1207	MELY-HSARK	1215
1090	4582789	1137	LELY-HAACKL	1146
1091	7489274	1220	LELY-HAARKL	1229
1092	2833389	1220	LELY-HAARKL	1229
1093	15221083	1017	LELY-HSARK	1025
1094	7489827	227	IELY-RSASQI	236
1095	4582783	858	EELY	861
1096	15717885	900	EDIY-Q	904
1097	15236819	1054	EELYTRSVSR	1063
1098	18461221	815	ENLY-QSA	821
1099	8901183	643	VDVY-RSIS	650
1100	17227527	482	LNIY	485
1101	16329217	483	VEAY	486
1102	2811062	468	KQAY-EQLIK	476
1103	7484399	433	VDVY-RSIS	440
1104	18309046	474	KELY-EELIK	482
1105	15903076	469	LDLY-HS	474
1106	17229371	448	DKLY-RS	453
1107	15900991	469	LDLY-HS	474
1108	16124067	470	LSLY-R	474
1109	16080147	469	QRIF-EQVTR	477
1110	16273270	470	RTLY	473
1111	15602409	470	HALY-Q	474
1112	15672681	477	IELY-Q	481
1113	17938870	487	MALF-ES 492
1114	16119514	466	MALF-ES	471
1115	15613648	462	TALY	465
1116	16331219	448	IKVY-K	452
1117	17366711	472	IELY-Q	476
1118	15805621	466	LELY-R	470
1119	15620537	448	KQLY-AEA	454
1120	15641730	475	MKMY-RLA	481
1122	15605532	469	LEIY-K	473
1123	15803938	470	RELY	473
1124	17367076	438	LELY-R	442
1125	16766821	470	RELY	473
1126	16762766	470	RELY	473
1127	15834801	469	LEIY-Q	473
1128	15966599	470	VSLY	473
1129	15618857	468	VNLY-QS 473
1130	17366350	430	REVY-REA	436
1131	15384987	624	-ELY	626
1132	2129898	742	EEVL-VAA	748
1133	2833384	742	EEVL-VAA	748
1134	15028467	681	-ELY	683
1135	15643657	474	VDLY-KKA	480

[0411]

TABLE XXXVI
Identities of the Accession Numbers used in Tables. XXXI-XXXIV.
Accession	Brief Description of sequences	score
Id.	producing significant alignments	(bits)	E-value
gi|7489826|pir||T01265	starch synthase DULL1 - maize >gi|30 . . .	969	0.0
gi|9502145|gb|AAF88000.1|	(AF258609) starch synthase III [A . . .	815	0.0
gi|9502143|gb|AAF87999.1|AF258608_1	(AF258608) starch synth . . .	811	0.0
gi|17646328|gb|AAL40942.1|AF432915_1	(AF432915) putative st . . .	808	0.0
gi|4582789|emb|CAB40374.1|	(AJ225088) Starch synthase isofo . . .	758	0.0
gi|7489274|pir|T07663	soluble starch synthase (EC 2.4.1. - ) . . .	752	0.0
gi|2833389|sp|Q43846|UGS4_SOLTU	Soluble glycogen [starch] s . . .	749	0.0
gi|15221083|ref|NP_172637.1|	(NM_101044) putative glycogen . . .	734	0.0
gi|7489827|pir|T01266	starch synthase DULL1 - maize (fragm . . .	495	e−139
gi|4582783|emb|CAB40375.1|	(AJ006752) starch synthase, isof . . .	394	e−108
gi|15717885|gb|AAK97773.1|	(AY044844) starch synthase isofo . . .	363	2e−99
gi|15236819|ref|NP_193558.1|	(NM_117934) starch synthase-li . . .	361	1e−98
gi|18461221|dbj|BAB84418.1|	(AP003292) putative starch synt . . .	337	2e−91
gi|8901183|gb|AAC17971.2|	(AF026422) soluble starch synthas . . .	303	4e−81
gi|17227527|ref|NP_484075.1|	(NC_003272) glycogen (starch) . . .	300	2e−80
gi|16329217|ref|NP_439945.1|	(NC_000911) glycogen (starch) . . .	265	9e−70
gi|2811062|sp|O08328|GLGA_BACST	Glycogen synthase (Starch [. . .	249	6e−65
gi|7484399|pir||T07926	probable starch synthase (EC 2.4.1.- . . .	232	5e−60
gi|18309046|ref|NP_560980.1|	(NC_003366) glycogen synthase . . .	226	5e−58
gi|15903076|ref|NP_358626.1|	(NC_003098) Glycogen synthase . . .	224	2e−57
gi|17229371|ref|NP_485919.1|	(NC_003272) glycogen synthase . . .	222	8e−57
gi|15900991|ref|NP_345595.1|	(NC_003028) glycogen synthase . . .	221	9e−57
gi|16124067|ref|NP_407380.1|	(NC_003143) glycogen synthase . . .	220	2e−56
gi|16080147|ref|NP_390973.1|	(NC_000964) starch (bacterial . . .	215	6e−55
gi|16273270|ref|NP_439511.1|	(NC_000907) glycogen synthase . . .	214	1e−54
gi|15602409|ref|NP_245481.1|	(NC_002663) GlgA [Pasteurella . . .	214	1e−54
gi|15672681|ref|NP_266855.1|	(NC_002662) glycogen synthase . . .	212	8e−54
gi|17938870|ref|NP_535658.1|	(NC_003306) glycogen synthase . . .	211	1e−53
gi|16119514|ref|NP_396220.1|	(NC_003064) AGR_pAT_410p [Agro . . .	211	1e−53
gi|15613648|ref|NP_241951.1|	(NC_002570) starch (bacterial . . .	209	4e−53
gi|16331219|ref|NP_441947.1|	(NC_000911) glycogen synthase . . .	209	4e−53
gi|17366711|sp|Q9CHM9|GLGA_LACLA	Glycogen synthase (Starch . . .	209	5e−53
gi|15805621|ref|NP_294317.1|	(NC_001263) glycogen synthase . . .	200	3e−50
gi|15620537|gb|AAL03921.1|U30252_9	(U30252) GlgA [Synechoco . . .	199	4e−50
gi|15641730|ref|NP_231362.1|	(NC_002505) glycogen synthase . . .	199	5e−50
gi|7489711|pir|T01209	ADPglucose - starch glucosyltransfera . . .	197	2e−49
gi|15605532|ref|NP_220318.1|	(NC_000117) Glycogen Synthase . . .	197	2e−49
gi|15803938|ref|NP_289974.1|	(NC_002655) glycogen synthase . . .	197	2e−49
gi|17367076|sp|Q9RWS1|GLGA_DEIRA	Glycogen synthase (Starch . . .	197	3e−49
gi|16766821|ref|NP_462436.1|	(NC_003197) glycogen synthase . . .	196	6e−49
gi|16762766|ref|NP_458383.1|	(NC_003198) glycogen synthase . . .	194	2e−48
gi|15834801|ref|NP_296560.1|	(NC_002620) glycogen synthase . . .	191	2e−47
gi|15966599|ref|NP_386952.1|	(NC_003047) PROBABLE GLYCOGEN . . .	189	4e−47
gi|15618857|ref|NP_225143.1|	(NC_000922) Glycogen Synthase . . .	189	5e−47
gi|17366350|sp|P58395|GLGA_THECA	Glycogen synthase (Starch . . .	187	1e−46
gi|15384987|emb|CAC59826.1|	(AJ308110) soluble starch synth . . .	187	2e−46
gi|2129898|pir||S61505	UDPglucose - starch glucosyltransfera . . .	185	9e−46
gi|2833384|sp|Q43093|UGS3_PEA	Glycogen [starch] synthase, c . . .	185	1e−45
gi|15028467|gb|AAK81729.1|AF395537_1	(AF395537) soluble sta . . .	185	1e−45
gi|15643657|ref|NP_228703.1|	(NC_000853) glycogen synthase . . .	184	2e−45
gi|15232051|ref|NP_186767.1|	(NM_110984) putative glycogen . . .	181	1e−44
gi|13476305|ref|NP_107875.1|	(NC_002678) glycogen synthase . . .	181	2e−44
gi|7489710|pir||T01208	ADPglucose - starch glucosyltransfera . . .	179	6e−44
gi|146139|gb|AAA23870.1|	(J02616) glycogen synthase (EC 2.4 . . .	179	6e−44
gi|14279432|gb|AAK58596.1|AF268969_2	(AF268969) glycogen sy . . .	179	7e−44
gi|15890897|ref|NP_356569.1|	(NC_003063) AGR_L_1562p [Agrob . . .	176	4e−43
gi|17366749|sp|Q9EUT5|GLGA_RHITR	Glycogen synthase (Starch . . .	175	7e−43
gi|16265159|ref|NP_437951.1|	(NC_003078) putative glycogen . . .	175	1e−42
gi|15895507|ref|NP_348856.1|	(NC_003030) Glycogen synthase, . . .	175	1e−42
gi|14495348|gb|AAK64284.1|AF383878_1	(AF383878) soluble sta . . .	173	4e−42
gi|15606118|ref|NP_213495.1|	(NC_000918) glycogen synthase . . .	173	4e−42
gi|7489695|pir||T06798	probable starch synthase (EC 2.4.1.- . . .	170	3e−41
gi|16265834|gb|AAL16661.1|AF419099_1	(AF419099) putative so . . .	169	6e−41
gi|8708896|gb|AAC17970.2|	(AF026421) soluble starch synthas . . .	167	2e−40
gi|6467503|gb|AAF13168.1|AF173900_1	(AF173900) granule boun . . .	166	3e−40
gi|7529653|emb|CAB86618.1|	(AJ269502) starch synthase IIa-1 . . .	162	5e−39
gi|3192881|gb|AAC19119.1|	(AF068834) starch synthase [Ipomo . . .	162	6e−39
gi|8953571|emb|CAB96626.1|	(AJ269503) starch synthase IIa-2 . . .	161	2e−38
gi|8953573|emb|CAB96627.1|	(AJ269504) starch synthase IIa-3 . . .	159	4e−38
gi|5825480|gb|AAD53263.1|AF155217_1	(AF155217) starch synth . . .	159	6e−38
gi|15237934|ref|NP_197818.1|	(NM_122336) soluble starch syn . . .	159	8e−38
gi|2833390|sp|Q43847|UGS3_SOLTU	Glycogen [starch] synthase, . . .	158	1e−37
gi|6690399|gb|AAF24126.1|AF121673_1	(AF121673) soluble star . . .	157	3e−37
gi|1549232|dbj|BAA07396.1|	(D38221) SSS1 [Oryza sativa] >gi . . .	156	4e−37
gi|5295947|dbj|BAA81848.1|	(AB026295) ESTs AU075322(C11109) . . .	156	4e−37
gi|2833377|sp|Q40739|UGS2_ORYSA	Soluble glycogen [starch] s . . .	156	4e−37
gi|5834407|gb|AAD53959.1|AF181035_3	(AF181035) glycogen syn . . .	154	2e−36
gi|17367070|sp|QPRNH6|GLGA_RHOSH	Glycogen synthase (Starch . . .	154	2e−36
gi|82478|pir||JQ0703	UDPglucose - starch glucosyltransferase . . .	154	2e−36
gi|15223331|ref|NP_174566.1|	(NM_103023) starch synthase, p . . .	152	8e−36
gi|2833382|sp|Q42968|UGST_ORYGL	Granule-bound glycogen [sta . . .	152	8e−36
gi|15983795|gb|AAL10494.1|	(AY056803) At1g32900/F9L11_8 [Ar . . .	150	2e−35
gi|7489712|pir||T01414	ADPglucose - starch glucosyltransfera . . .	150	2e−35
gi|297424|emb|CAA46294.1|	(X65183) glycogen (starch) syntha . . .	150	3e−35
gi|136758|sp|P19395|UGST_ORYSA	Granule-bound glycogen [star . . .	150	3e−35
gi|7798551|gb|AAC61675.2|	(AF031162) granule-bound starch s . . .	150	3e−35
gi|15451566|gb|AAK98690.1|AC069158_2	(AC069158) Putative st . . .	148	1e−34
gi|12019656|gb|AAD45815.2|	(AF168786) soluble starch syntha . . .	148	1e−34
gi|5441242|dbj|BAA82346.1|	(AB029546) granule-bound starch . . .	147	3e−34
gi|6318538|gb|AAF06936.1|AF110373_1	(AF110373) granule-boun . . .	146	5e−34
gi|6318540|gb|AAF06937.1|AF110374_1	(AF110374) granule-boun . . .	145	7e−34
gi|6624287|dbj|BAA88512.1|	(AB029064) starch synthase (GBSS . . .	145	1e−33
gi|4760582|dbj|BAA77351.1|	(AB019623) starch Synthase (GBSS . . .	145	1e−33
gi|4760584|dbj|BAA77352.1|	(AB019624) starch synthase (GBSS . . .	144	2e−33
gi|15626365|emb|CAC69955.1|	(AJ345045) granule-bound starch . . .	144	2e−33
gi|15597361|ref|NP_250855.1|	(NC_002516) probable glycogen . . .	144	2e−33
gi|4588609|gb|AAD26156.1|AF113844_1	(AF113844) granule-boun . . .	144	2e−33
gi|7484400|pir||T07924	probable starch Synthase (EC 2.4.1.—. . .	144	3e−33
gi|6624285|dbj|BAA88511.1|	(AB029063) starch synthase (GBSS . . .	144	3e−33
gi|6624281|dbj|BAA88509.1|	(AB029061) starch synthase (GBSS . . .	144	3e−33
gi|17736918|gb|AAL41028.1|	(AF250137) mutant granule bound . . .	144	3e−33
gi|11037536|gb|AAG27624.1|AF286320_1	(AF286320) granule bou . . .	142	6e−33
gi|136755|sp|P09842|UGST_HORVU	Granule-bound glycogen [star . . .	140	2e−32
gi|18652407|gb|AAL77109.1|AF474373_6	(AF474373) granule-bou . . .	140	2e−32
gi|4760580|dbj|BAA77350.1|	(AB019622) starch synthase (GBSS . . .	140	3e−32
gi|136765|sp|P27736|UGST_WHEAT	Granule-bound glycogen [star . . .	139	5e−32
gi|6624283|dbj|BAA88510.1|	(AB029062) starch synthase (GBSS . . .	139	7e−32
gi|228210|prf||1718316A	granule-bound starch synthase [Sola . . .	138	1e−31
gi|4588607|gb|AAD26155.1|AF113843_1	(AF113843) granule-boun . . .	138	1e−31
gi|16716335|gb|AAC17969.3|	(AF026420) granule-bound starch . . .	137	3e−31
gi|3493111|gb|AAD03013.1|	(AF079293) granule-bound starch s . . .	135	1e−30
gi|3493079|gb|AAD02997.1|	(AF079277) granule-bound starch s . . .	135	1e−30
gi|602594|emb|CAA58220.1|	(X83220) starch (bacterial glycog . . .	135	1e−30
gi|12003285|gb|AAG43519.1|AF210699_1	(AF210699) granule-bou . . .	135	1e−30
gi|267196|sp|Q00775|UGST_SOLTU	Granule-bound glycogen [star . . .	134	2e−30
gi|3493113|gb|AAD03014.1|	(AF079294) granule-bound starch s . . .	134	2e−30
gi|3493115|gb|AAD03015.1|	(AF079295) granule-bound starch s . . .	132	7e−30
gi|136757|sp|P04713|UGST_MAIZE	Granule-bound glycogen [star . . .	132	9e−30
gi|3493119|gb|AAD03017.1|	(AF079297) granule-bound starch s . . .	131	1e−29
gi|3493055|gb|AAD02985.1|	(AF079265) granule-bound starch s . . .	131	1e−29
gi|12278481|gb|AAG48981.1|	(AY010994) granule-bound starch . . .	131	1e−29
gi|3493107|gb|AAD03011.1|	(AF079291) granule-bound starch s . . .	131	1e−29
gi|12278501|gb|AAG48991.1|	(AY011004) granule-bound starch . . .	131	2e−29
gi|2833383|sp|Q43092|UGST_PEA	Granule-bound glycogen [starc . . .	130	2e−29
gi|12278491|gb|AAG48986.1|	(AY010999) granule-bound starch . . .	130	2e−29
gi|3493121|gb|AAD03018.1|	(AF079298) granule-bound starch s . . .	130	4e−29
gi|12278477|gb|AAG48979.1|	(AY010992) granule-bound starch . . .	129	5e−29
gi|12278479|gb|AAG48980.1|	(AY010993) granule-bound starch . . .	129	6e−29
gi|12278485|gb|AAG48983.1|	(AY010996) granule-bound starch . . .	129	6e−29
gi|3493057|gb|AAD02986.1|	(AF079266) granule-bound starch s . . .	129	8e−29
gi|12278489|gb|AAG48985.1|	(AY010998) granule-bound starch . . .	129	9e−29
gi|12278495|gb|AAG48988.1|	(AY011001) granule-bound starch . . .	129	9e−29
gi|3493081|gb|AAD02998.1|	(AF079278) granule-bound starch s . . .	129	9e−29
gi|3493075|gb|AAD02995.1|	(AF079275) granule-bound starch s . . .	129	1e−28
gi|3493005|gb|AAD02960.1|	(AF079240) granule-bound starch s . . .	128	1e−28
gi|3493085|gb|AAD03000.1|	(AF079280) granule-bound starch s . . .	128	1e−28
gi|12278417|gb|AAG48949.1|	(AY010962) granule-bound starch . . .	128	2e−28
gi|3493027|gb|AAD02971.1|	(AF079251) granule-bound starch s . . .	128	2e−28
gi|2833381|sp|Q42857|UGST_IPOBA	Granule-bound glycogen [sta . . .	127	2e−28
gi|3493077|gb|AAD02996.1|	(AF079276) granule-bound starch s . . .	127	2e−28
gi|12278473|gb|AAG48977.1|	(AY010990) granule-bound starch . . .	127	2e−28
gi|12278487|gb|AAG48984.1|	(AY010997) granule-bound starch . . .	127	2e−28
gi|12278509|gb|AAG48995.1|	(AY011008) granule-bound starch . . .	127	2e−28
gi|3493089|gb|AAD03002.1|	(AF079282) granule-bound starch s . . .	127	2e−28
gi|12278514|gb|AAG48997.1|	(AY011011) granule-bound starch . . .	127	2e−28
gi|3493087|gb|AAD03001.1|	(AF079281) granule-bound starch s . . .	127	3e−28
gi|3493065|gb|AAD02990.1|	(AF079270) granule-bound starch s . . .	127	3e−28
gi|3493093|gb|AAD03004.1|	(AF079284) granule-bound starch s . . .	127	3e−28
gi|12278415|gb|AAG48948.1|	(AY010961) granule-bound starch . . .	127	3e−28
gi|3493095|gb|AAD03005.1|	(AF079285) granule-bound starch s . . .	127	3e−28
gi|12278463|gb|AAG48972.1|	(AY010985) granule-bound starch . . .	127	3e−28
gi|3493051|gb|AAD02983.1|	(AF079263) granule-bound starch s . . .	127	3e−28
gi|3493013|gb|AAD02964.1|	(AF079244) granule-bound starch s . . .	127	3e−28
gi|3493099|gb|AAD03007.1|	(AF079287) granule-bound starch s . . .	126	4e−28
gi|3493049|gb|AAD02982.1|	(AF079262) granule-bound starch s . . .	126	4e−28
gi|3493097|gb|AAD03006.1|	(AF079286) granule-bound starch s . . .	126	4e−28
gi|3493031|gb|AAD02973.1|	(AF079253) granule-bound starch s . . .	126	4e−28
gi|3493007|gb|AAD02961.1|	(AF079241) granule-bound starch s . . .	126	4e−28
gi|12278421|gb|AAG48951.1|	(AY010964) granule-bound starch . . .	126	4e−28
gi|3493091|gb|AAD03003.1|	(AF079283) granule-bound starch s . . .	126	4e−28
gi|3493059|gb|AAD02987.1|	(AF079267) granule-bound starch s . . .	126	4e−28
gi|12278453|gb|AAG48967.1|	(AY010980) granule-bound starch . . .	126	4e−28
gi|12278451|gb|AAG48966.1|	(AY010979) granule-bound starch . . .	126	4e−28
gi|13377473|gb|AAK20725.1|	(AF318769) granule-bound starch . . .	126	4e−28
gi|3493019|gb|AAD02967.1|	(AF079247) granule-bound starch s . . .	126	5e−28
gi|12278443|gb|AAG48962.1|	(AY010975) granule-bound starch . . .	126	6e−28
gi|12278471|gb|AAG48976.1|	(AY010989) granule-bound starch . . .	125	7e−28
gi|3493067|gb|AAD02991.1|	(AF079271) granule-bound starch s . . .	125	7e−28
gi|13774484|gb|AAK38881.1|	(AF353519) granule-bound starch . . .	125	7e−28
gi|12278493|gb|AAG48987.1|	(AY011000) granule-bound starch . . .	125	8e−28
gi|3493103|gb|AAD03009.1|	(AF079289) granule-bound starch s . . .	125	8e−28
gi|12278435|gb|AAG48958.1|	(AY010971) granule-bound starch . . .	125	9e−28
gi|12278423|gb|AAG48952.1|	(AY010965) granule-bound starch . . .	125	1e−27
gi|13194734|gb|AAK15529.1|	(AF331953) granule-bound starch . . .	125	1e−27
gi|3493071|gb|AAD02993.1|	(AF079273) granule-bound starch s . . .	125	1e−27
gi|12278419|gb|AAG48950.1|	(AY010963) granule-bound starch . . .	125	1e−27
gi|12278429|gb|AAG48955.1|	(AY010968) granule-bound starch . . .	124	2e−27
gi|17940620|gb|AAL49697.1|	(AF445158) granule-bound starch . . .	124	2e−27
gi|12278457|gb|AAG48969.1|	(AY010982) granule-bound starch . . .	124	2e−27
gi|3493061|gb|AAD02988.1|	(AF079268) granule-bound starch s . . .	124	2e−27
gi|3493069|gb|AAD02992.1|	(AF079272) granule-bound starch s . . .	124	2e−27
gi|12278449|gb|AAG48965.1|	(AY010978) granule-bound starch . . .	124	2e−27
gi|3493025|gb|AAD02970.1|	(AF079250) granule-bound starch s . . .	124	2e−27
gi|12278507|gb|AAG48994.1|	(AY011007) granule-bound starch . . .	124	2e−27
gi|12278511|gb|AAG48996.1|	(AY011009) granule-bound starch . . .	124	2e−27
gi|3493073|gb|AAD02994.1|	(AF079274) granule-bound starch s . . .	124	2e−27
gi|3493037|gb|AAD02976.1|	(AF079256) granule-bound starch s . . .	124	2e−27
gi|3493117|gb|AAD03016.1|	(AF079296) granule-bound starch s . . .	124	2e−27
gi|12278427|gb|AAG48954.1|	(AY010967) granule-bound starch . . .	124	2e−27
gi|12278425|gb|AAG48953.1|	(AY010966) granule-bound starch . . .	124	2e−27
gi|3493035|gb|AAD02975.1|	(AF079255) granule-bound starch s . . .	124	2e−27
gi|12278497|gb|AAG48989.1|	(AY011002) granule-bound starch . . .	124	2e−27
gi|3493021|gb|AAD02968.1|	(AF079248) granule-bound starch s . . .	124	2e−27
gi|3492999|gb|AAD02957.1|	(AF079237) granule-bound starch s . . .	124	3e−27
gi|3493041|gb|AAD02978.1|	(AF079258) granule-bound starch s . . .	124	3e−27
gi|13774482|gb|AAK38880.1|	(AF353518) granule-bound starch . . .	124	3e−27
gi|3493023|gb|AAD02969.1|	(AF079249) granule-bound starch s . . .	124	3e−27
gi|12278437|gb|AAG48959.1|	(AY010972) granule-bound starch . . .	123	3e−27
gi|3493083|gb|AAD02999.1|	(AF079279) granule-bound starch s . . .	123	3e−27
gi|3493101|gb|AAD03008.1|	(AF079288) granule-bound starch s . . .	123	4e−27
gi|12278475|gb|AAG48978.1|	(AY010991) granule-bound starch . . .	123	4e−27
gi|12278469|gb|AAG48975.1|	(AY010988) granule-bound starch . . .	123	4e−27
gi|13774486|gb|AAK38882.1|	(AF353520) granule-bound starch . . .	123	4e−27
gi|3493015|gb|AAD02965.1|	(AF079245) granule-bound starch s . . .	123	4e−27
gi|12278411|gb|AAG48946.1|	(AY010959) granule-bound starch . . .	123	5e−27
gi|12278503|gb|AAG48992.1|	(AY011005) granule-bound starch . . .	123	5e−27
gi|12278505|gb|AAG48993.1|	(AY011006) granule-bound starch . . .	123	5e−27
gi|12278413|gb|AAG48947.1|	(AY010960) granule-bound starch . . .	123	5e−27
gi|13377475|gb|AAK20726.1|	(AF318770) granule-bound starch . . .	123	5e−27
gi|3493011|gb|AAD02963.1|	(AF079243) granule-bound starch s . . .	122	6e−27
gi|3493001|gb|AAD02958.1|	(AF079238) granule-bound starch s . . .	122	6e−27
gi|12278431|gb|AAG48956.1|	(AY010969) granule-bound starch . . .	122	7e−27
gi|3493053|gb|AAD02984.1|	(AF079264) granule-bound starch s . . .	122	7e−27
gi|13774480|gb|AAK38879.1|	(AF353517) granule-bound starch . . .	122	7e−27
gi|17940628|gb|AAL49701.1|	(AF445162) granule-bound starch . . .	122	8e−27
gi|3493047|gb|AAD02981.1|	(AF079261) granule-bound starch s . . .	122	9e−27
gi|15054954|gb|AAK82785.1|AF292516_1	(AF292516) granule-bou . . .	122	9e−27
gi|3493003|gb|AAD02959.1|	(AF079239) granule-bound starch s . . .	122	9e−27
gi|12278433|gb|AAG48957.1|	(AY010970) granule-bound starch . . .	122	9e−27
gi|3493009|gb|AAD02962.1|	(AF079242) granule-bound starch s . . .	122	1e−26
gi|15054956|gb|AAK82786.1|AF292517_1	(AF292517) granule-bou . . .	122	1e−26
gi|3493043|gb|AAD02979.1|	(AF079259) granule-bound starch s . . .	122	1e−26
gi|15054922|gb|AAK82769.1|AF292500_1	(AF292500) granule-bou . . .	122	1e−26
gi|15054984|gb|AAK82800.1|AF292531_1	(AF292531) granule-bou . . .	122	1e−26
gi|17940655|gb|AAL49713.1|	(AF445177) granule-bound starch . . .	122	1e−26
gi|15054990|gb|AAK82803.1|AF292534_1	(AF292534) granule-bou . . .	122	1e−26
gi|15054930|gb|AAK82773.1|AF292504_1	(AF292504) granule-bou . . .	122	1e−26
gi|15054960|gb|AAK82788.1|AF292519_1	(AF292519) granule-bou . . .	122	1e−26
gi|12278499|gb|AAG48990.1|	(AY011003) granule-bound starch . . .	121	1e−26
gi|15054968|gb|AAK82792.1|AF292523_1	(AF292523) granule-bou . . .	121	1e−26
gi|3492995|gb|AAD02955.1|	(AF079235) granule-bound starch s . . .	121	1e−26
gi|17432494|gb|AAL38185.1|	(AY062271) granule-bound starch . . .	121	1e−26
gi|17940659|gb|AAL49715.1|	(AF445179) granule-bound starch . . .	121	2e−26
gi|3493017|gb|AAD02966.1|	(AF079246) granule-bound starch s . . .	121	2e−26
gi|17940626|gb|AAL49700.1|	(AF445161) granule-bound starch . . .	121	2e−26
gi|17940622|gb|AAL49698.1|	(AF445159) granule-bound starch . . .	121	2e−26
gi|3493033|gb|AAD02974.1|	(AF079254) granule-bound starch s . . .	120	2e−26
gi|17940636|gb|AAL49705.1|	(AF445166) granule-bound starch . . .	120	2e−26
gi|17940639|gb|AAL49706.1|	(AF445168) granule-bound starch . . .	120	2e−26
gi|15054970|gb|AAK82793.1|AF292524_1	(AF292524) granule-bou . . .	120	3e−26
gi|3493063|gb|AAD02989.1|	(AF079269) granule-bound starch s . . .	120	3e−26
gi|3493045|gb|AAD02980.1|	(AF079260) granule-bound starch s . . .	120	4e−26
gi|17940634|gb|AAL49704.1|	(AF445165) granule-bound starch . . .	120	4e−26
gi|3493039|gb|AAD02977.1|	(AF079257) granule-bound starch s . . .	120	5e−26
gi|17940630|gb|AAL49702.1|	(AF445163) granule-bound starch . . .	119	5e−26
gi|17940632|gb|AAL49703.1|	(AF445164) granule-bound starch . . .	119	6e−26
gi|17940648|gb|AAL49710.1|	(AF445173) granule-bound starch . . .	119	7e−26
gi|17940624|gb|AAL49699.1|	(AF445160) granule-bound starch . . .	119	8e−26
gi|17940677|gb|AAL49723.1|	(AF445189) granule-bound starch . . .	118	2e−25
gi|17940644|gb|AAL49708.1|	(AF445171) granule-bound starch . . .	118	2e−25

[0412]

Glycosyl transferase family group1 (pfam 00534)domain (“GLYTR”) of maize
GBSS
NKEALQAEVGLPVDRNIPLVAFIGRLEEQ	Seq ID. No. 1136
KGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFERMLMSAEEKFPGKVRAVVKFNAALA
HHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIEGKTGFHMGRLS
VDCNVVEPADVKKVATTLQRAIK
Glycosyl transferase family group1 (pfam 00534)domain (“GLYTR”) of maize
SSI
LPIRPDVPLIGFIGRLDYQKGIDLIQLIIPDLMREDVQFVMLGSGDPELEDWMRSTESIFKDKFRGWV	Seq ID. No. 1137
GFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHATGGLRDTVENFNPF
GENGEQGTGWAFAPLTTENMFVDIANCNIYIQGTQVLLGRANEARHVKRLHVGPCR
Glycosyl transferase family group1 (pfam 00534)domain (GLYTR) of maize
SSIIa
KAALQRELGLEVRDDVPLLGFIGRLDGQKGVDIIGDAMPWIAGQDVQLVMLGTGRADLERMLQHLEREH	Seq ID. No. 1138
PNKVRGWVGFSVPMAHRITAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVGGLRDTVAPFDPFGD
AGLGWTFDRAEANKLIEALR
Glycosyl transferase family group1 (pfam 00534)domain (GLYTR) of maize
SSIIb
QVRDDVPLIGFIGRLDHQKGVDIIADAIHWIAGQDVQLVMLGTGRADLEDMLRRFESEHSDKVRAWVGFS	Seq ID. No. 1139
VPLAHRITAGADILLMPSRFEPCGLNQLYAMAYGTVPVVHAVGGLRDTVAPFDPFNDTGLGWTFDRAEA
NRMID
Glycosyl transferase family group1 (pfam 00534)domain (GLYTR) of maize
DU1
PVV GIVTRLTAQK GIHLIKHAIHRTLERNGQVV LLGSAPDSRI QADFVNLANT LHGVNHGQVR	Seq ID. No. 1140
LSLTYDEPLS HLIYAGSDFILVPSIFEPCG LTQLVAMRYG TIPIVRKTGG LFDTVFDVDN
Nucleotide sequence of maize GBSS
1	CAGCGACCTA TTACACAGCC CGCTCGGGCC CGCGACGTCG GGACACATCT TCTTCCCCCT	Seq ID. No. 1141
61	TTTGGTGAAG CTCTGCTCGC AGCTGTCCGG CTCCTTGGAC GTTCGTGTGG CAGATTCATC
121	TGTTGTCTCG TCTCCTGTGC TTCCTGGGTA GCTTGTGTAG TGGAGCTGAC ATGGTCTGAG
181	CAGGCTTAAA ATTTGCTCGT AGACGAGGAG TACCAGCACA GCACGTTGCG GATTTCTCTG
241	CCTGTGAAGT GCAACGTCTA GGATTGTCAC ACGCCTTGGT CGCGTCGCGT CGCGTCGCGT
301	CGATGCGGTG GTGAGCAGAG CAGCAACAGC TGGGCGGCCC AACGTTGGCT TCCGTGTCTT
361	CGTCGTACGT ACGCGCGCGC CGGGGACACG CAGCAGAGAG CGGAGAGCGAGCCGTGCACG
421	GGGAGGTGGT GTGGAAGTGG AGCCGCGCGC CCGGCCGCCC GCGCCCGGTGGGCAACCCAA
481	AAGTACCCAC GACAAGCGAA GGCGCCAAAG CGATCCAAGC TCCGGAACGC AACAGCATGC
541	GTCGCGTCGG AGAGCCAGCC ACAAGCAGCC GAGAACCGAA CCGGTGGGCG ACGCGTCATG
601	GGACGGACGC GGGCGACGCT TCCAAACGGG CCACGTACGC CGGCGTGTGC GTGCGTGCAG
661	ACGACAAGCC AAGGCGAGGC AGCCCCCGAT CGGGAAAGCG TTTTGGGCGC GAGCGCTGGC
721	GTGCGGGTCA GTCGCTGGTG CGCAGTGCCG GGGGGAACGG GTATCGTGGG GGGCGCGGGC
781	GGAGGAGAGC GTGGCGAGGG CCGAGAGCAG CGCGCGGCCG GGTCACGCAA CGCGCCCCAC
841	GTACTGCCCT CCCCCTCCGC GCGCGCTAGA AATACCGAGG CCTGGACCGG GGGGGGGCCC
901	CGTCACATCC ATCCATCGAC CGATCGATCG CCACAGCCAA CACCACCCGC CGAGGCGACG
961	CGACAGCCGC CAGGAGGAAG GAATAAACTC ACTGCCAGCC AGTGAAGGGG GAGAAGTGTA
1021	CTGCTCCGTC GACCAGTGCG CGCACCGCCC GGCAGGGCTG CTCATCTCGT CGACGACCAG
1081	GTTCTGTTCC GTTCCGATCC GATCCGATCC TGTCCTTGAG TTTCGTCCAG ATCCTGGCGC
1141	GTATCTGCGT GTTTGATGAT CCAGGTTCTT CGAACCTAAA TCTGTCCGTG CACACGTCTT
1201	TTCTCTCTCT CCTACGCAGT GGATTAATCG GCATGGCGGC TCTGGCCACG TCGCAGCTCG
1261	TCGCAACGCG CGCCGGCCTG GGCGTCCCGG ACGCGTCCAC GTTCCGCCGC GGCGCCGCGC
1321	AGGGCCTGAG GGGGGCCCGG GCGTCGGCGG CGGCGGACAC GCTCAGCATG CGGACCAGCG
1381	CGCGCGCGGC GCCCAGGCAC CAGCAGCAGG CGCGCCGCGG GGGCAGGTTC CCGTCGCTCG
1441	TCGTGTGCGC CAGCGCCGGC ATGAACGTCG TCTTCGTCGG CGCCGAGATG GCGCCGTGGA
1501	GCAAGACCGG CGGCCTCGGC GACGTCCTCG GCGGCCTGCC GCCGGCCATG GCCGTAAGCG
1561	CGCGCACCGA GACATGCATC CGTTGGATCG CGTCTTCTTC GTGCTCTTGC CGCGTGCATG
1621	ATGCATGTGT TTCCTCCTGG CTTGTGTTCG TGTATGTGAC GTGTTTGTTC GGGCATGCAT
1681	GCAGGCGAAC GGGCACCGTG TCATGGTCGT CTCTCCCCGC TACGACCAGT ACAAGGACGC
1741	CTGGGACACC AGCGTCGTGT CCGAGGTACG GCCACCGAGA CCAGATTCAG ATCACAGTCA
1801	CACACACCGT CATATGAACC TTTCTCTGCT CTGATGCCTG CAACTGCAAA TGCATGCAGA
1861	TCAAGATGGG AGACGGGTAC GAGACGGTCA GGTTCTTCCA CTGCTACAAG CGCGGAGTGG
1921	ACCGCGTGTT CGTTGACCAC CCACTGTTCC TGGAGAGGGT GAGACGAGAT CTGATCACTC
1981	GATACGCAAT TACCACCCCA TTGTAAGCAG TTACAGTGAG CTTTTTTTCC CCCCGGCCTG
2041	GTCGCTGGTT TCAGGTTTGG GGAAAGACCG AGGAGAAGAT CTACGGGCCT GTCGCTGGAA
2101	CGGACTACAG GGACAACCAG CTGCGGTTCA GCCTGCTATG CCAGGTCAGG ATGGCTTGGT
2161	ACTACAACTT CATATCATCT GTATGCAGCA GTATACACTG ATGAGAAATG CATGCTGTTC
2221	TGCAGGCAGC ACTTGAAGCT CCAAGGATCC TGAGCCTCAA CAACAACCCA TACTTCTCCG
2281	GACCATACGG TAAGAGTTGC AGTCTTCGTA TATATATCTG TTGAGCTCGA GAATCTTCAC
2341	AGGAAGCGGC CCATCAGACG GACTGTCATT TTACACTGAC TACTGCTGCT GCTCTTCGTC
2401	CATCCATACA AGGGGAGGAC GTCGTGTTCG TCTGCAACGA CTGGCACACC GGCCCTCTCT
2461	CGTGCTACCT CAAGAGCAAC TACCAGTCCC ACGGCATCTA CAGGGACGCA AAGGTTGCCT
2521	TCTCTGAACT GAACAACGCC GTTTTCGTTC TCCATGCTCG TATATACCTC GTCTGGTAGT
2581	GGTGGTGCTT CTCTGAGAAA CTAACTGAAA CTGACTGCAT GTCTGTCTGA CCATCTTCAC
2641	GTACTACCAG ACCGCTTTCT GCATCCACAA CATCTCCTAC CAGGGCCGGT TCGCCTTCTC
2701	CGACTACCCG GAGCTGAACC TCCCGGAGAG ATTCAAGTCG TCCTTCGATT TCATCGACGG
2761	GTCTGTTTTC CTGCGTGCAT GTGAACATTC ATGAATGGTA ACCCACAACT GTTCGCGTCC
2821	TGCTGGTTCA TTATCTGACC TGATTGCATT ATTGCAGCTA CGAGAAGCCC GTGGAAGGCC
2881	GGAAGATCAA CTGGATGAAG GCCGGGATCC TCGAGGCCGA CAGGGTCCTC ACCGTCAGCC
2941	CCTACTACGC CGAGGAGCTC ATCTCCGGCA TCGCCAGGGG CTGCGAGCTC GACAACATCA
3001	TGCGCCTCAC CGGCATCACC GGCATCGTCA ACGGCATGGA CGTCAGCGAG TGGGACCCCA
3061	GCAGGGACAA GTACATCGCC GTGAAGTACG ACGTGTCGAC GGTGAGCTGG CTAGCTCTGA
3121	TTCTGCTGCC TGGTCCTCCT GCTCATCATG CTGGTTCGGT ACTGACGCGG CAAGTGTACG
3181	TACGTGCGTG CGACGGTGGT GTCCGGTTCA GGCCGTGGAG GCCAAGGCGC TGAACAAGGA
3241	GGCGCTGCAG GCGGAGGTCG GGCTCCCGGT GGACCGGAAC ATCCCGCTGG TGGCGTTCAT
3301	CGGCAGGCTG GAAGAGCAGA AGGGCCCCGA CGTCATGGCG GCCGCCATCC CGCAGCTCAT
3361	GGAGATGGTG GAGGACGTGC AGATCGTTCT GCTGGTACGT GTGCGCCGGC CGCCACCCGG
3421	CTACTACATG CGTGTATCGT TCGTTCTACT GGAACATGCG TGTGAGCAAC GCGATGGATA
3481	ATGCTGCAGG GCACGGGCAA GAAGAAGTTC GAGCGCATGC TCATGAGCGC CGAGGAGAAG
3541	TTCCCAGGCA AGGTGCGCGC CGTGGTCAAG TTCAACGCGG CGCTGGCGCA CCACATCATG
3601	GCCGGCGCCG ACGTGCTCGC CGTCACCAGC CGCTTCGAGC CCTGCGGCCT CATCCAGCTG
3661	CAGGGGATGC GATACGGAAC GGTACGAGAG AAAAAAAAAA TCCTGAATCC TGACGAGAGG
3721	GACAGAGACA GATTATGAAT GCTTCATCGA TTTGAATTGA TTGATCGATG TCTCCCGCTG
3781	CGACTCTTGC AGCCCTGCGC CTGCGCGTCC ACCGGTGGAC TCGTCGACAC CATCATCGAA
3841	GGCAAGACCG GGTTCCACAT GGGCCGCCTC AGCGTCGACG TAAGCCTAGC TCTGCCATGT
3901	TCTTTCTTCT TTCTTTCTGT ATGTATGTAT GAATCAGCAC CGCCGTTCTT GTTTCGTCGT
3961	CGTCCTCTCT TCCCAGTGTA ACGTCGTGGA GCCGGCGGAC GTCAAGAAGG TGGCCACCAC
4021	ATTGCAGCGC GCCATCAAGG TGGTCGGCAC GCCGGCGTAC GAGGAGATGG TGAGGAACTG
4081	CATGATCCAG GATCTCTCCT GGAAGGTACG TACGCCCGCC CCGCCCCGCC CCGCCAGAGC
4141	AGAGCGCCAA GATCGACCGA TCGACCGACC ACACGTACGC GCCTCGCTCC TGTCGCTGAC
4201	CGTGGTTTAA TTTGCGAAAT GCGCAGGGCC CTGCCAAGAA CTGGGAGAAC GTGCTGCTCA
4261	GCCTCGGGGT CGCCGGCGGC GAGCCAGGGG TCGAAGGCGA GGAGATCGCG CCGCTCGCCA
4321	AGGAGAACGT GGCCGCGCCC TGAAGAGTTC GGCCTGCAGG GCCCCTGATC TCGCGCGTGG
4381	TGCAAAGATG TTGGGACATC TTCTTATATA TGCTGTTTCG TTTATGTGAT ATGGACAAGT
4441	ATGTGTAGCT GCTTGCTTGT GCTAGTGTAA TGTAGTGTAG TGGTGGCCAG TGGCACAACC
4501	TAATAAGCGC ATGAACTAAT TGCTTGCGTG TGTAGTTAAG TACCGATCGG TAATTTTATA
4561	TTGCGAGTAA ATAAATGGAC CTGTAGTGGT GGAGTAAATA ATCCCTGCTG TTCGGTGTTC
4621	TTATCGCTCC TCGTATAGAT ATTATATAGA GTACATTTTT CTCTCTCTGA ATCCTACGTT
4681	TGTGAAATTT CTATATCATT ACTGTAAAAT TTCTGCGTTC CAAAAGAGAC CATAGCCTAT
4741	CTTTGGCCCT GTTTGTTTCG GCTTCTGGCA GCTTCTGGCC ACCAAAAGCT GCTGCGGACT
Nucleotide sequence of maize SSI
1	GAATTCGCGG CCGCCTTATT TCTGGTTGGC CACATACATC ATCCAAAAAA CTTTATTATT	Seq ID. No. 1142
61	GAATTACAAC TAATAAGCAA TCTAAAAGAG GGCACCACCA ATGATGTGTT GTTGGTAGGA
121	GGCCGCTGGG TCTGTCAAAG CAAGITGGAC AAAGGGCAAC AATTGTTGTA GTTGTAAGAG
181	GGTTGCGGGG TTAGCCGCAA ACTGCTGGTA GAAAGGCAGC AACTGTTGCT GTGTCAAGAA
241	GGAAGCACGG TTTGCTGCAG CTGTTGTGCC CTGATGGTTT GTACGAATGA CTGCACCAAA
301	GATAGGGCTG GCGATTGTTG AAACAACAAG GGCGATAAAG GTATGTTGCT TGCTGCGATT
361	GCTTGTTGAA GCCTATATGG TTGAAGAGCT GGGTTTTCAC ATATTGAAGC TATAATTGAT
421	GGAAGGTATG GGGGAAGAAG GGAAGCTATA GGAGCTTGTG AGCATTGAGG GAAAATTGTC
481	GCGTTAGCAA CACATGTAGA AAGAGCAAGG AGCATAAGGA GGGAAAATAT CTTGGTCGCC
541	ATTGTTGCGC GCGATCCACG GCCCCCGCCC CCCGCGCTCC TGTCTGCTCT CCCTCTCCGC
601	AATGGCGACG CCCTCGGCCG TGGGCGCCGC GTGCCTCCTC CTCGCGCGGG CCGCCTGGCC
661	GGCCGCCGTC GGCGACCGGG CGCGCCCGCG GCGGCTCCAG CGCGTGCTGC GCCGCCGGTG
721	CGTCGCGGAG CTGAGCAGGG AGGGGCCCGC GCCGCGCCCG CTGCCACCCG CGCTGCTGGC
781	GCCCCCGCTC GTGCCCGGCT TCCTCGCGCC GCCGGCCGAG CCCACGGGTG AGCCGGCATC
841	GACGCCGCCG CCCGTGCCCG ACGCCGGCCT GGGGGACCTC GGTCTCGAAG CTGAAGGGAT
901	TGCTGAAGGT TCCATCGATA ACACAGTAGT TGTGGCAAGT GAGCAAGATT CTGAGATTGT
961	GGTTGGAAAG GAGCAAGCTC GAGCTAAAGT AACACAAAGC ATTGTCTTTG TAACCGGCGA
1021	AGCTTCTCCT TATGCAAAGT CTGGGGGTCT AGGAGATGTT TGTGGTTCAT TGCCAGTTGC
1081	TCTTGCTGCT CGTGGTCACC GTGTGATGGT TGTAATGCCC AGATATTTAA ATGGTACCTC
1041	CGATAAGAAT TATGCAAATG CATTTTACAC AGAAAAACAC ATTCGGATTC CATGCTTTGG
1201	CGGTGAACAT GAAGTTACCT TCTTCCATGA GTATAGAGAT TCAGTTGACT GGGTGTTTGT
1261	TGATCATCCC TCATATCACA GACCTGGAAA TTTATATGGA GATAAGTTTG GTGCTTTTGG
1321	TGATAATCAG TTCAGATACA CACTCCTTTG CTATGCTGCA TGTGAGGCTC CTTTGATCCT
1381	TGAATTGGGA GGATATATTT ATGGACAGAA TTGCATGTTT GTTGTCAATG ATTGGCATGC
1441	CAGTCTAGTG CCAGTCCTTC TTGCTGCAAA ATATAGACCA TATGGTGTTT ATAAAGACTC
1501	CCGCAGCATT CTTGTAATAC ATAATTTAGC ACATCAGGGT GTAGAGCCTG CAAGCACATA
1561	TCCTGACCTT GGGTTGCCAC CTGAATGGTA TGGAGCTCTG GAGTGGGTAT TCCCTGAATG
1621	GGCGAGGAGG CATGCCCTTG ACAAGGGTGA GGCAGTTAAT TTTTTGAAAG GTGCAGTTGT
1681	GACAGCAGAT CGAATCGTGA CTGTCAGTAA GGGTTATTCG TGGGAGGTCA CAACTGCTGA
1741	AGGTGGACAG GGCCTCAATG AGCTCTTAAG CTCCAGAAAG AGTGTATTAA ACGGAATTGT
1801	AAATGGAATT GACATTAATG ATTGGAACCC TGCCACAGAC AAATGTATCC CCTGTCATTA
1861	TTCTGTTGAT GACCTCTCTG GAAAGGCCAA ATGTAAAGGT GCATTGCAGA AGGAGCTGGG
1921	TTTACCTATA AGGCCTGATG TTCCTCTGAT TGGCTTTATT GGAAGGTTGG ATTATCAGAA
1981	AGGCATTGAT CTCATTCAAC TTATCATACC AGATCTCATG CGGGAAGATG TTCAATTTGT
2041	CATGCTTGGA TCTGGTGACC CAGAGCTTGA AGATTGGATG AGATCTACAG AGTCGATCTT
2101	CAAGGATAAA TTTCGTGGAT GGGTTGGATT TAGTGTTCCA GTTTCCCACC GAATAACTGC
2161	CGGCTGCGAT ATATTGTTAA TGCCATCCAG ATTCGAACCT TGTGGTCTCA ATCAGCTATA
2221	TGCTATGCAG TATGGCACAG TTCCTGTTGT CCATGCAACT GGGGGCCTTA GAGATACCGT
2281	GGAGAACTTC AACCCTTTCG GTGAGAATGG AGAGCAGGGT ACAGGGTGGG CATTCGCACC
2341	CCTAACCACA GAAAACATGT TTGTGGACAT TGCGAACTGC AATATCTACA TACAGGGAAC
2401	ACAAGTCCTC CTGGGAAGGG CTAATGAAGC GAGGCATGTC AAAAGACTTC ACGTGGGACC
2461	ATGCCGCTGA ACAATACGAA CAAATCTTCC AGTGGGCCTT CATCGGATCG ACCCGATGTT
2521	CAATGGAAAA AAGGGACCAA AGTTGGTTGG TTCCTTGAAG ATTATCAGTT CATCATCCTA
2581	TAGTAAGCTG AATGATGAAA GAAAACCCCT GTACATTACA TGGAAGGCAG ACCGGCTATT
2641	GGCTCCATTG CTCCAATGTC TGCTTTGGCT GCCTTGCCTC GATGGACCGG ATGCAGTGAG
2701	GAATCCAGNC GAACGACAGT TTTGAAGGAT AGGAAGGGGA GCTGGAAGCA GTCACGCAGG
2761	CAGGCAAGCC TTCGCCGTTA ATTCATATGG AACAAGCTGG AGTCAGTTTC TGCTGTGCCA
2821	CTCACTGTTT ACCTTAAGAT TATTACCTGT GTTGTTCTCC TTTGCTCGTT AGGGCTGATA
2881	ACATAATGAC TCATTAAGAA TATAATTCAC TCTGCCTCGT TTTTAATCTT AAGTGAAGTC
2941	GAGATCTACT TCGTCATTCC TTCCCCGTTT AAAAAAAAAA AAAAAAAAAA A
Nucleotide sequence of maize SSIIa
1	GCGGCCGCTT TTTTTTAAAA TCTGTGGAGA AGGCTTTATT ATTAGCTCAT AATACTTTTG	Seq ID. No. 1143
61	ATGCATCAAT AGGCCTCTCC CACCTCACAT TAGTCTCAAC ATTGACTTCA TATTATACAA
121	CCCTAAGAAG GTTGATCGGC TGCAACAATT GAAATCTAAA GACTACGTCA AGCGCTTCCC
181	TCTTGTGATA TCATCAGTTT GCTGGAACCT TGTAGCTTCC GCACTGGAAG CCATGCTCAA
241	ATGGCCTCTT ACACTGCTCA GCAACATACA CAACCTTCTC CATGCACACA ACCTTCTCAG
301	TTTCTTTGGT GACCTTGGAG CACAAGCAAG AAACGTTIGC CTTCTTGATG ACACCACAGC
361	AAGCATCCGA AGGAGGGACC TTTGGATTAG CTGGGAACAT CACGTATGTC TTGCAGTCAC
421	TTATCAGGCT TTGGAGGTCA TTCTCACAGT CTCCATCAGC ATGAGAGCCT ATGGCAAACA
481	TAGCTGCTAC AAAGGAGAGG GCTAAGAACA GGCTCAACAG TTTTGCCATG TTTACTAGTT
541	CTTGGGGGAC GCCTATTGAA AGCTGTCCGC TGCCCACCGC GTGCGCTGCG CTCCCGTGCG
601	GCACTGCCGG CGGCGCGACC CCGCGCCCAT TGGACGAGCT TCCGCCCCGC CCCGATCGAT
661	CCCGCCGCCA TGTCGTCGGC GGCCGTGTCG TCCTCTTCCT CCACCTTCTT CCTCGCGCTC
721	GCCTCCGCCT CCCCCGGGGG CCGCAGGCGG GCTAGGGTCG GCTCCTCGCC GTTCCACACC
781	GGCGCCAGCC TGAGTTTCGC GTTCTGGGCG CCACCGTCGC CGCCGCGCGC GCCCCGGGAC
841	GCAGCGCTGG TGCGCGCTGA GGCTGAGGCC GGGGGCAAGG ACGCGCCGCC GGAGAGGAGC
901	GGCGACGCCG CCAGGTTGCC CCGCGCTCGG CGCAATGCGG TCTCCAAACG GAGGGATCCT
961	CTTCAGCCGG TCGGCCGGTA CGGCTCCGCG ACGGGAAACA CGGCCAGGAC CGGCGCCGCG
1021	TCCTGCCAGA ACGCCGCATT GGCGGACGTT GAGATCAAGT CCATCGTCGC CGCGCCGCCG
1081	ACGAGCATAG TGAAGTTCCC AGCGCCGGGC TACAGGATGA TCCTTCCCTC TGGGGACATA
1041	GCGCCGGAGA CTGTCCTCCC AGCCCCGAAG CCACTGCATG AATCGCCTGC GGTTGACGGA
1201	GATTCAAATG GAATTGCACC TCCTACAGTT GAGCCATTAG TACAGGAGGC CACTTGGGAT
1261	TTCAAGAAAT ACATCGGTTT TGACGAGCCT GACGAAGCGA AGGATGATTC CAGGGTTGGT
1321	GCAGATGATG CTGGTTCTTT TGAACATTAT GGGGACAATG ATTCTGGGCC TTTGGCCGGG
1381	GAGAATGTTA TGAACGTGAT CGTGGTGGCT GCTGAATGTT CTCCATGGTG CAAAACAGGT
1441	GGTCTTGGAG ATGTTGTGGG AGCTTTACCC AAGGCTTTAG CGAGAAGAGG ACATCGTGTT
1501	ATGGTTGTGG TACCAAGGTA TGGGGACTAT GTGGAAGCCT TTGATATGGG AATCCGGAAA
1561	TACTACAAAG CTGCAGGACA GGACCTAGAA GTGAACTATT TCCATGCATT TATTGATGGA
1621	GTCGACTTTG TGTTCATTGA TGCCCCTCTT TTCCGGCACC GTCAAGATGA CATATATGGG
1681	GGAAGTAGGC AGGAAATCAT GAAGCGCATG ATTTTGTTTT GCAAGGTTGC TGTTGAGGTT
1741	CCTTGGCACG TTCCATGCGG TGGTGTGTGC TACGGAGATG GAAATTTGGT GTTCATTGCC
1801	AATGATTGGC ACACTGCACT CCTGCCTGTT TATCTGAAGG CATATTACAG AGACCATGGG
1861	TTAATGCAGT ACACTCGCTC CGTCCTCGTC ATACATAACA TCGCCCACCA GGGCCGTGGT
1921	CCTGTAGATG AATTCCCGTA CATGGACTTG CCTGAACACT ACCTTCAACA TTTCGAGCTG
1981	TACGATCCCG TCGGTGGCGA GCACGCCAAC ATCTTTGCCG CGGGTCTGAA GATGGCAGAC
2041	CGGGTGGTGA CTGTCAGCCG CGGCTACCTG TGGGAGCTGA AGACAGTGGA AGGCGGCTGG
2101	GGCCTCCACG ACATCATCCG TTCTAACGAC TGGAAGATCA ATGGCATCGT GAACGGCATC
2161	GACCACCAGG AGTGGAACCC CAAGGTGGAC GTGCACCTGC GGTCGGACGG CTACACCAAC
2221	TACTCCCTCG AGACACTCGA CGCTGGAAAG CGGCAGTGCA AGGCGGCCCT GCAGCGGGAG
2281	CTGGGCCTGG AAGTGCGCGA CGACGTGCCG CTGCTCGGCT TCATCGGGCG TCTGGATGGA
2341	CAGAAGGGCG TGGACATCAT CGGGGACGCG ATGCCGTGGA TCGCGGGGCA GGACGTGCAG
2401	CTGGTGATGC TGGGCACCGG GCGCGCCGAC CTGGAACGAA TGCTGCAGCA CTTGGAGCGG
2461	GAGCATCCCA ACAAGGTGCG CGGGTGGGTC GGGTTCTCGG TGCCTATGGC GCATCGCATC
2521	ACGGCGGGCG CCGACGTGCT GGTGATGCCT TCCCGCTTCG AGCCCTGCGG GCTGAACCAG
2581	CTCTACGCGA TGGCGTACGG CACCGTCCCT GTGGTGCACG CCGTGGGCGG GCTCAGGGAC
2641	ACCGTGGCGC CGTTCGACCC GTTCGGCGAC GCCGGGCTCG GGTGGACTTT TGACCGCGCC
2701	GAGGCCAACA AGCTGATCGA GGCGCTCAGG CACTGCCTCG ACACGTACCG GAAGTACGGG
2761	GAGAGCTGGA AGAGTCTCCA GGCGCGCGGC ATGTCGCAGG ACCTCAGCTG GGACCACGCG
2821	GCTGAGCTCT ACGAGGACGT CCTTGTCAAG GCCAAGTACC AGTGG
Nucleotide sequence of maize SSIIb
1	GCGGCCGCCT GGTAGGCGCT GGTACAAGCG GAAGCAGCAG TAGCGTGAGG CATCCCCATG	Seq ID. No. 1144
61	CCGGGGGCAA TCTCTTCCTC GTCGTCGGCT TTTCTCCTCC CCGTCGCGTC CTCCTCGCCG
121	CGGCGCAGGC GGGGCAGTGT GGGTGCTGCT CTGCGCTCGT ACGGCTACAG CGGCGCGGAG
181	CTGCGGTTGC ATTGGGCGCG GCGGGGCCCG CCTCAGGATG GAGCGGCGTC GGTACGCGCC
241	GCAGCGGCAC CGGCCGGGGG CGAAAGCGAG GAGGCAGCGA AGAGCTCCTC CTGGTCCCAG
301	GCGGGCGCTG TTCAGGGCAG CACGGCCAAG GCTGTGGATT CTGCTTCACC TCCCAATCCT
361	TTGACATCTG CTCCGAAGCA AAGTCAGAGC GCTGCAATGC AAAACGGAAC GAGTGGGGGC
421	AGCAGCGCGA GCACCGCCGC GCCGGTGTCC GGACCCAAAG CTGATCATCC ATCAGCTCCT
481	GTCACCAAGA GAGAAATCGA TGCCAGTGCG GTGAAGCCAG AGCCCGCAGG TGATGATGCT
541	AGACCGGTGG AAAGCATAGG CATCGCTGAA CCGGTGGATG CTAAGGCTGA TGCAGCTCCG
601	GCTACAGATG CGGCGGCGAG TGCTCCTTAT GACAGGGAGG ATAATGAACC TGGCCCTTTG
661	GCTGGGCCTA ATGTGATGAA CGTCGTCGTG GTGGCTTCTG AATGTGCTCC TTTCTGCAAG
721	ACAGGTGGCC TTGGAGATGT CGTGGGTGCT TTGCCTAAGG CTCTGGCGAG GAGAGGACAC
781	CGTGTTATGG TCGTGATACC AAGATATGGA GAGTATGCCG AAGCCCGGGA TTTAGGTGTA
841	AGGAGACGTT ACAAGGTAGC TGGACAGGAT TCAGAAGTTA CTTATTTTCA CTCTTACATT
901	GATGGAGTTG ATTTTGTATT CGTAGAAGCC CCTCCCTTCC GGCACCGGCA CAATAATATT
961	TATGGGGGAG AAAGATTGGA TATTTTGAAG CGCATGATTT TGTTCTGCAA GGCCGCTGTT
1021	GAGGTTCCAT GGTATGCTCC ATGTGGCGGT ACTGTCTATG GTGATGGCAA CTTAGTTTTC
1081	ATTGCTAATG ATTGGCATAC CGCACTTCTG CCTGTCTATC TAAAGGCCTA TTACCGGGAC
1041	AATGGTTTGA TGCAGTATGC TCGCTCTGTG CTTGTGATAC ACAACATTGC TCATCAGGGT
1201	CGTGGCCCTG TAGACGACTT CGTCAATTTT GACTTGCCTG AACACTACAT CGACCACTTC
1261	AAACTGTATG ACAACATTGG TGGGGATCAC AGCAACGTTT TTGCTGCGGG GCTGAAGACG
1321	GCAGACCGGG TGGTGACCGT TAGCAATGGC TACATGTGGG AGCTGAAGAC TTCGGAAGGC
1381	GGGTGGGGCC TCCACGACAT CATAAACCAG AACGACTGGA AGCTGCAGGG CATCGTGAAC
1441	GGCATCGACA TGAGCGAGTG GAACCCCGCT GTGGACGTGC ACCTCCACTC CGACGACTAC
1501	ACCAACTACA CGTTCGAGAC GCTGGACACC GGCAAGCGGC AGTGCAAGGC CGCCCTGCAG
1561	CGGCAGCTGG GCCTGCAGGT CCGCGACGAC GTGCCACTGA TCGGGTTCAT CGGGCGGCTG
1621	GACCACCAGA AGGGCGTGGA CATCATCGCC GACGCGATCC ACTGGATCGC GGGGCAGGAC
1681	GTGCAGCTCG TGATGCTGGG CACCGGGCGG GCCGACCTGG AGGACATGCT GCGGCGGTTC
1741	GAGTCGGAGC ACAGCGACAA GGTGCGCGCG TGGGTGGGGT TCTCGGTGCC CCTGGCGCAC
1801	CGCATCACGG CGGGCGCGGA CATCCTGCTG ATGCCGTCGC GGTTCGAGCC GTGCGGGCTG
1861	AACCAGCTCT ACGCCATGGC GTACGGGACC GTGCCCGTGG TGCACGCCGT GGGGGGGCTC
1921	CGGGACACGG TGGCGCCGTT CGACCCGTTC AACGACACCG GGCTCGGGTG GACGTTCGAC
1981	CGCGCGGAGG CGAACCGGAT GATCGACGCG CTCTCGCACT GCCTCACCAC GTACCGGAAC
2041	TACAAGGAGA GCTGGCGCGC CTGCAGGGCG CGCGGCATGG CCGAGGACCT CAGCTGGGAC
2101	CACGCCGCCG TGCTGTATGA GGACGTGCTC GTCAAGGCGA AGTACCAGTG GTGAGCGAAT
2161	TAATTGGCGA CGCGACGCCG CTCCTGTCGC AGGACCTGGA CGTTATTTAG AAGGCTCTTC
2221	TCCCTGGCGG CTTTGATGCG TGCGTCGCAT TTGCGCCGGG CGGACGGGCG ACGGTGGTTG
2281	GCCTACCGCC TACGTCGGCT GCGTGCCCTG GGAATTTGGG CGGGCACGAT GATGCCACTG
2341	GGCACCGGGC GCGGGGTAGT ATGATATGAA ACCGACGGCG ATGGAGATGA GGCGCATGGC
2401	ATTTTCCCAC TGATAAATGG GGAGTTGTAT GCTACTTTAA TATCGCCACT CCTGTTAGTA
2461	TTTATATTGA TGGCGGCCGC
Nucleotide sequence of maize Du1
1	GAATTCCCTA GTTCAGAGAA AGAAAGAAGT TGAGAATGAG AAGCAAGTGA GGCGCGTTTG	Seq ID. No. 1145
61	CTGGGAAGTG GTTCTTGTGA GGTTTAGGAG TTCACCCTTT TTTTCTTCCC CTTCTAGAAA
121	TGGAGATGGT CCTACGGTCG CAGAGCCCTC TCTGCCTTCG GAGTGGGCCG GTGCTCATTT
181	TTCGACCAAC CGTCGCGGGC GGAGGAGGOG GCACTCAGTC TTTGTTGAGG ACTACCAGAT
241	TTGCGAGAAG AAGGGTCATT CGATGCGTTG TAGCAAGTCC AGGTTGTCCT AATAGGAAAT
301	CTAGGACAGC GTCTCCCAAC GTAAAAGTAG CTGCTTATAG CAACTATGCG CCAAGACTCC
361	TCGTTGAGTC AAGCTCCAAG AAGAGCGAAC ACCATGATAG CAGCAGACAC CGTGAAGAAA
421	CTATTGATAC ATACAATGQG CTGTCAGGTT CTGATGCAGC AGAATTGACA AGTAATAGAG
481	ATGTAGAAAT TGAAGTGGAT TTGCAGCACA TTTCTGAGGA GGAATTGCCA GGAAAAGTAT
541	CGATTAATGC ATCATTAGGA GAAATGGAAA CAGTGGATGA AGCTGAGGTC GAGGAGGATA
601	AGTTTGAGGT AGATACCTCA GGAATTGTAT TGCGCAATGT TGCAGTTCGG GAAGTGGATC
661	CAAAGGATGA ACATAATGCT AAAGATGTAT TTGTGGTAGA TTCGTCAGGA ACTGCACCAG
721	ATAATGCTGC AGTGGAGGAA GTGGTAGATG AAGCTGAGGT TGAAGAGGAT ATGGTTGATG
781	TGGATATCTT GGGACTTGAC TTGAATAATG CAACGATCGA GGAAATTGAT TTGATGGAAG
841	AGGCTTTACT GGAGAACTTC GACGTGGATT CACCAGGCAA TGCTTCTAGT GGTCGAACCT
901	ATGGGGGTGT GGATGAGTTG GGTGAGCTGC CTTCAACATC CGTGGATTGC ATCGCCATTA
961	ACGGAAAACG TAGAAGTTTG AAGCCTAAGC CCTTGCCAAT TGTCAGGTTC CAGGAACAAG
1021	AACAGATAGT TTTAAGCATT GTTGACGAAG AAGGGTTGAT TGCTAGTTCA TGTGAAGAAG
1081	GCCAACCGGT GGTAGATTAC GATAAGCAAG AGGAAAACTC TACCGCTTTC GATGAACAGA
1041	AGCAATTAAC TGATGATTTC CCTGAAGAAG GCATATCTAT AGTTCACTTC CCTGAGCCAA
1201	ACAATGATAT TGTTGGATCC TCAAAATTCT TGGAGCAAAA ACAAGAATTG GATGGTTCTT
1261	ATAAACAAGA TCGATCAACC ACTGGATTGC ATGAACAAGA TCAGTCTGTT GTTAGTTCAC
1321	ACGGACAAGA TAAATCAATT GTTGGTGTGC CTCAGCAAAT CCAGTACAAT GATCAATCTA
1381	TTGCTGGTTC TCATAGACAA GATCAATCAA TTGCCGGTGC ACCTGAGCAA ATCCAATCCG
1441	TTGCTGGCTA TATAAAACCA AATCAATCTA TTGTTGGTTC TTGTAAACAA CATGAATTGA
1501	TTATTCCTGA GCCTAAGAAA ATCGAATCCA TCATCAGTTA CAATGAAATA GATCAATCTA
1561	TTGTTGGTTC TCACAAACAA GACAAATCTG TTGTTAGTGT GCCTGAGCAA ATCCAATCCA
1621	TTGTTAGTCA CAGCAAACCA AATCAATCTA CTGTTGATTC TTATAGACAA GCTGAATCAA
1681	TTATTGGTGT GCCTGAGAAA GTCCAATCCA TCACCAGTTA CGATAAACTA GACCAGTCCA
1741	TTGTTGGTTC TCTTAAACAA GATGAGCCTA TTATTAGCGT GCCTGAGAAA ATCCAATCCA
1801	TTGTCCATTA CACTAAACCA AATCAGTCTA TTGTTGGCTT GCCCAAACAA CAACAATCAA
1861	TTGTTCATAT CGTTGAACCA AAACAGTCCA TAGATGGTTT CCCTAAACAA GATCTATCAA
1921	TCGTTGGTAT CTCCAATGAG TTTCAAACAA AGCAACTGGC TACTGTTGGG ACTCATGATG
1981	GATTGCTTAT GAAGGGTGTG GAAGCTAAGG AGACATCTCA AAAGACTGAA GGGGATACAC
2041	TTCAGGCAAC GTTCAATGTC GACAACTTGT CACAGAAACA GGAAGGCTTA ACTAAAGAAG
2101	CAGACGAGAT AACAATTATT GAGAAAATCA ATGATGAAGA CCTTGTGATG ATTGAAGAAC
2161	AGAAAAGCAT AGCCATGAAT GAAGAACAGA CGATTGTTAC CGAAGAAGAC ATTCCAATGG
2221	CTAAGGTTGA GATAGGAATT GACAAGGCCA AATTTTTACA TCTGCTTTCT GAAGAAGAGA
2281	GTTCATGGGA TGAAAATGAA GTGGGAATAA TTGAGGCTGA TGAACAGTAT GAAGTCGATG
2341	AGACATCTAT GTCCACTGAA CAAGATATCC AGGAATCACC TAATGATGAT TTGGATCCAC
2401	AAGCACTATG GAGTATGCTT CAAGAGCTTG CTGAAAAAAA TTATTCGCTG GGAAACAAGT
2461	TGTTTACTTA TCCAGATGTA TTGAAAGCTG ATTCAACAAT TGATCTCTAT TTCAATCGTG
2521	ATCTATCAGC TGTGGCCAAT GAGCCTGATG TACTTATCAA AGGAGCATTC AATGGGTGGA
2581	AGTGGAGATT TTTCACTGAA AAATTGCACA AGAGCGAGCT GGCAGGGGAC TGGTGGTGCT
2641	GCAAACTATA CATTCCTAAG CAGGCATACA GAATGGACTT TGTGTTTTTT AACGGACACA
2701	CGGTATATGA AAATAATAAC AATAATGATT TCGTGATACA AATAGAAAGC ACCATGGATG
2761	AAAATTTATT TGAGGATTTC TTGGCTGAAG AAAAGCAACG AGAACTTGAG AACCTTGCAA
2821	ATGAGGAAGC TGAAAGGAGG AGACAAACTG ATGAGCAGCG GCGAATGGAG GAAGAAAGGG
2881	CCGCAGATAA AGCTGACAGG GTACAAGCCA AGGTTGAGGT AGAGACGAAG AAGAATAAAT
2941	TGTGCAATGT ATTGGGTTTA GCCAGAGCTC CTGTTGATAA TTTATGGTAC ATTGAGCCCA
3001	TCACGACTGG ACAAGAGGCT ACTGTCAGAT TGTATTATAA CATAAACTCA AGACCTCTAG
3061	TTCACAGTAC TGAGATATGG ATGCATGGTG GCTATAACAA TTGGATTGAT GGACTCTCTT
3121	TTGCTGAAAG GCTTGTTCAT CATCATGACA AAGATTGTGA TTGGTGGTTT GCAGATGTTG
3181	TCGTGCCTGA AAGAACATAT GTATTGGACT GGGTTTTTGC TGACGGCCCA CCAGGGAGTG
3241	CAAGGAATTA TGACAACAAT GGAGGACATG ATTTTCATGC TACCCTTCCA AATAACATGA
3301	CTGAGGAAGA GTATTGGATG GAAGAAGAAC AAAGGATCTA TACAAGGCTT CAACAAGAGA
3361	GGAGGGAAAG GGAGGAGGCT ATTAAAAGGA AGGCTGAGAG AAATGCAAAA ATGAAAGCTG
3421	AGATGAAGGA AAAGACTATG AGAATGTTCC TGGTTTCTCA GAAACACATT GTTTACACCG
3481	AACCACTTGA AATACATGCT GGAACTACTA TTGATGTGCT TTATAATCCT TCTAATACAG
3541	TTCTAACTGG AAAGCCAGAG GTTTGGTTTC GATGTTCCTT TAATCGTTGG ATGTATCCAG
3601	GTGGGGTGTT GCCACCTCAG AAGATGGTAC AAGCAGAAAA TGGTTCACAC CTAAAAGCAA
3661	CAGTTTACGT TCCACGAGAT GCCTATATGA TGGACTTCGT TTTCTCGGAG TCAGAAGAAG
3721	GTGGAATTTA TGATAACAGA AATGGGTTAG ACTATCATAT TCCTGTTTTT GGGTCAATTG
3781	CAAAGGAACC ACCTATGCAC ATTGTCCACA TTGCTGTTGA GATGGCACCA ATCGCAAAGG
3841	TTGGAGGTCT TGGTGATGTT GTCACTAGTC TTTCACGTGC TGTGCAAGAT TTAGGACACA
3901	ATGTGGAGGT TATTCTTCCA AAGTACGGTT GCTTGAATCT AAGCAATGTC AAGAATCTAC
3961	AAATCCATCA GAGTTTTTCT TGGGGTGGTT CTGAAATAAA TGTGTGGCGT GGACTAGTCG
4021	AAGGCCTTTG TGTTTACTTC CTGGAACCTC AAAATGGGAT GTTTGGAGTC GGATATGTAT
4081	ATGGCAGGGA CGATGACCGC CGATTTGGCT TCTTCTGTCG TTCTGCTCTA GAGTTTCTCC
4141	TCCAAAGTGG ATCTTCTCCG AACATAATAC ATTGCCATGA TTGGTCAAGT GCTCCTGTTG
4201	CCTGGCTACA CAAGGAAAAC TACGCGAAGT CTAGCTTGGC AAACGCACGG GTGGTATTCA
4261	CCATCCACAA TCTTGAATTT GGAGCGCATC ATATTGGCAA AGCAATGAGA TATTGTGATA
4321	AAGCAACAAC TGTCTCTAAT ACATATTCAA AGGAAGTGTC AGGTCATGGT GCCATAGTTC
4381	CTCATCTTGG GAAATTCTAT GGCATTCTCA ATGGAATTGA TCCGGATATA TGGGATCCGT
4441	ACAATGACAA CTTTATCCCG GTCCACTACA CTTGTGAGAA TGTGGTTGAA GGCAAGAGGG
4501	CTGCTAAGAG GGCACTGCAG CAGAAGTTTG GGTTACAGCA AATCGATGTC CCCGTCGTAG
4561	GAATCGTCAC TCGCCTGACA GCCCAAAAGG GGATCCACCT GATCAAGCAT GCGATTCACC
4621	GTACACTCGA ACGGAACGGA CAGGTGGTTT TGCTTGGTTC AGCGCCGGAC TCTCGAATCC
4681	AAGCTGATTT TGTCAACCTG GCGAATACGC TCCACGGCGT AAACCATGGG CAAGTGAGGC
4741	TTTCCTTGAC CTACGACGAG CCTCTCTCGC ATCTGATATA CGCTGGCTCT GACTTCATTC
4801	TGGTCCCATC TATATTTGAG CCTTGCGGCC TAACTCAGCT CGTCGCCATG CGGTATGGAA
4861	CCATCCCGAT TGTCCGCAAG ACTGGAGGGC TCTTCGACAC TGTCTTCGAT GTGGACAATG
4921	ACAAGGAACG AGCCCGAGAT CGAGGCCTTG AGCCCAACGG GTTTAGCTTT GACGGAGCTG
4981	ATAGCAACGG TGTTGACTAC GCGCTGAACA GGGCGATCTC AGCTTGGTTC GATGCCCGGA
5041	GCTGGTTCCA CTCCCTTTGC AAGAGAGTCA TGGAGGAGGA CTGGTCGTGG AACCGACCTG
5101	CCCTCGACTA CATCGAGCTC TACCGTTCAG CGTCCAAATT GTAATAATCC AAACAACGGC
5161	CAATGTAGTG TGTTGTCTGC AGGTCTCAGA TGCAGCCATT CAGCTTTTGC AGGTTCCTGG
5221	GCATTGCTGT ACAGCCTCCT TGTCTTTAGT TAGCTCCATT CCCCGAGGAG CACAGTGCAA
5281	TTTTTTATCC TCAGTTATTA TGCATAGATT GTCTCAGTAG AATGCTTTCT TCGGGCATGT
5341	ATGTTTGTTT CCTCTGTTGT TGAATTCTGG TGTTAAGTCG CGTATAGGAA TCTACAGGAA
5401	ATGAAAAAGT CCATTTCCTG CGTCAACCTT TTAGGGCTAC CATGCACATG AGACCTTTCA
5461	AGTGCAAAGA ATATTAGGAC TAGACTACTA GTATGTGAAC TCTATTTTTC CAAGAGATTT
5521	CAATTTTTCC AATGAAAAAT AAACTAATTT TTCTTGGAAA AATGGAAATC CCTTGGAAAA
5581	ATGGGGTTCC CAAACTAGCC CGTAGAGTAT AGATCATAGA ATTGGTCTAG TGGTTCCTCG
5641	AGAGAGAAAA AAACATAGAC TTTTCTTGTC ATATGCTTAT TTAAGTTTAT TTTGTACAAA
5701	CTTTGAGAAC CTTCAAAAAC ACCCCAATGG CTGGTTAAGT GACCAGGGAA ATAAAGAGGA
5761	TCTATAGGGA GGAATCCCCC GCCTCTCTCT CACAGATGTT GCCTAGCACC GGCCAGCCTC
5821	ATCCGTCCAG TGGAATTAAG GTTGGTTGCG ACGACAGCCC ATCAATGGAA ACCAACCTCG
5881	TGCCCCGTGC CGGGATCTAC CTTCCTTCCT CACCACCACG CCGATCTCAC CTTCCATAGG
5941	AGCTTCCTAT GCACTGTTAC CTATTATAGG TACATGACAT TGTACATCTT TGTATGAACT
6001	TACATCAATG CCAAAAATCC GGAATTC
C-terminal end (“CTEND”) of maize GBSS;
K V V G T P A Y E E M V R N C M I Q D L S W K G P A K N W E N V L L S L G V A	Seq ID. No. 1146
G G E P G V E G E E I A P L A K E N V A A P
C-terminal end (“CTEND”) of maize SSI;
G E Q G T G W A F A P L T T E N M F V D I A N C N I Y I Q G T	Seq ID. No. 1147
Q V L L G R A N E A R H V K R L H V G P C R
C-terminal end (“CTEND”) of maize SSIIa;
H C L D T Y R K Y G E S W K S L Q A R G M S Q D L S W D H A A E L	Seq ID. No. 1148
Y E D V L V K A K Y Q W
C-terminal end (“CTEND”) of maize SSIIb;
N R M I D A L S H C L T T Y R N Y K E S W R A C R A R G M A E D L S W D H A A	Seq ID. No. 1149
V L Y E D V L V K A K Y Q W
C-terminal end (“CTEND”) of maize SSIII (Du-1);
N D K E R A R D R G L E P N G F S F D G A D S N G V D Y A L N R A I S A W F D	Seq ID. No. 1150
A R S W F H S L C K R V M E Q D W S W N R P A L D Y I E L Y R S A S K L

[0413]

TABLE XXXVII
List of Glucan Transferases with Different Database Accession Identifications
1:   NP_385953 1. PHOSPHOGLYCEROL TRANSFERASE, CYCLIC BETA-1,2-GLUCAN MODIFICATION PROTEIN
     [Sinorhizobium meliloti]
     gi|15965600|ref|NP_385953.1|[15965600]
2:   Q9SD76
     Alpha-glucan phosphorylase, H isozyme (Starch phosphorylase H)
     gi|14916634|sp|Q9SD76|PHSH_ARATH[14916634]
3:   Q9LKJ3
     Alpha-glucan phosphorylase, H isozyme (Starch phosphorylase H)
     gi|14916632|sp|Q9LKJ3|PHSH_WHEAT[14916632]
4:   Q9PKU9
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|14194917|sp|Q9PKU9|MALQ_CHLMU[14194917]
5:   Q9Z1E4
     Glycogen [starch] synthase, muscle
     gi|12230199|sp|Q9Z1E4|GYS1_MOUSE[12230199]
6:   Q9VFC8
     Putative glycogen [starch] synthase
     gi|12230129|sp|Q9VFC8|GYS_DROME[12230129]
7:   Q9U2D9
     Putative glycogen [starch] synthase
     gi|12230128|sp|Q9U2D9|GYS_CAEEL[12230128]
8:   Q9Y704
     Cell wall alpha-1,3-glucan synthase mok14
     gi|11386958|sp|Q9Y704|MOKE_SCHPO[11386958]
9:   P27472
     Glycogen [starch] synthase, isoform 2
     gi|1717973|sp|P27472|GYS2_YEAST[1717973]
10:  P54840
     Glycogen [starch] synthase, liver
     gi|1717972|sp|P54840|GYS2_HUMAN[1717972]
11:  P54859
     Glycogen [starch] synthase, brain
     gi|1717968|sp|P54859|GYS3_MOUSE[1717968]
12:  P13807
     Glycogen [starch] synthase, muscle
     gi|1351366|sp|P13807|GYS1_HUMAN[1351366]
13:  P17625
     Glycogen [starch] synthase, liver
     gi|136764|sp|P17625|GYS2_RAT[136764]
14:  P23337
     Glycogen [starch] synthase, isoform 1
     gi|136753|sp|P23337|GYS1_YEAST[136753]
15:  P04045
     Alpha-1,4 glucan phosphorylase, L-1 isozyme, chloroplast precursor (Starch phosphorylase L-1)
     gi|130173|sp|P04045|PHS1_SOLTU[130173]
16:  P27598
     Alpha-1,4 glucan phosphorylase, L isozyme, chloroplast precursor (Starch phosphorylase L)
     gi|130172|sp|P27598|PHSL_IPOBA[130172]
17:  O93869
     Glycogen [starch] synthase
     gi|12230121|sp|O93869|GYS_NEUCR[12230121]
18:  P80099
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|6226644|sp|P80099|MGTA_THEMA[6226644]
19:  O87172
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|6225654|sp|O87172|MALQ_THEAQ[6225654]
20:  P72785
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|6225653|sp|P72785|MALQ_SYNY3[6225653]
21:  O53932
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|6225652|sp|O53932|MALQ_MYCTU[6225652]
22:  O84089
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|6225650|sp|O84089|MALQ_CHLTR[6225650]
23:  O66937
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|6225647|sp|O66937|MALQ_AQUAE[6225647]
24:  P13031
     Glycogen phosphorylase
     gi|2851413|sp|P13031|PHSG_ECOLI[2851413]
25:  P53536
     Alpha-1,4 glucan phosphorylase, L isozyme, chloroplast precursor (Starch phosphorylase L)
     gi|2506470|sp|P53536|PHSL_VICFA[2506470]
26:  P53535
     Alpha-1,4 glucan phosphorylase, L-2 isozyme, chloroplast precursor (Starch phosphorylase L-2)
     gi|1730557|sp|P53535|PHS2_SOLTU[1730557]
27:  P52981
     1,4-alpha-glucan branching enzyme (Glycogen branching enzyme)
     gi|1707936|sp|P52981|GLGB_SYNY3[1707936]
28:  Q10625
     Probable 1,4-alpha-glucan branching enzyme (Glycogen branching enzyme)
     gi|1707934|sp|Q10625|GLGB_MYCTU[1707934]
29:  P45176
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|1170869|sp|P45176|MALQ_HAEIN[1170869]
30:  P15977
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|1170868|sp|P15977|MALQ_ECOLI[1170868]
31:  P30924
     1,4-alpha-glucan branching enzyme (Starch branching enzyme) (Q-enzyme)
     gi|1169912|sp|P30924|GLGB_SOLTU[1169912]
32:  P45177
     1,4-alpha-glucan branching enzyme (Glycogen branching enzyme)
     gi|1169910|sp|P45177|GLGB_HAEIN[1169910]
33:  P38678
     Glucan synthase-1 (1,3-beta-glucan synthase) (UDP-glucose-1,3-beta-D-glucan glucosyltransferase)
     gi|729636|sp|P38678|GS1_NEUCR[729636]
34:  P39118
     1,4-alpha-glucan branching enzyme (Glycogen branching enzyme)
     gi|729579|sp|P39118|GLGB_BACSU[729579]
35:  Q04446
     1,4-alpha-glucan branching enzyme (Glycogen branching enzyme) (Brancher enzyme)
     gi|544388|sp|Q04446|GLGB_HUMAN[544388]
36:  Q06801
     4-alpha-glucanotransferase, chloroplast precursor (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|544184|sp|Q06801|DPEP_SOLTU[544184]
37:  Q06119
     Diacylglycerol kinase (DAGK) (Diglyceride kinase) (DGK)
     gi|462437|sp|Q06119|KDGL_RHIME[462437]
38:  P32434
     Type I protein geranylgeranyltransferase beta subunit (Type I protein geranyl-geranyltransferase beta subunit) (GGTase-I-beta) (PGGT)
     gi|416853|sp|P32434|CWG2_SCHPO[416853]
39:  P29851
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|266497|sp|P29851|MALQ_STRPN[266497]
40:  P07762
     1,4-alpha-glucan branching enzyme (Glycogen branching enzyme)
     gi|121296|sp|P07762|GLGB_ECOLI[121296]
41:  O32462
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|18202015|sp|O32462|MALQ_THELI[18202015]
42:  O32450
     4-alpha-glucanotransferase (Amylomaltase) (Disproportionating enzyme) (D-enzyme)
     gi|18202014|sp|O32450|MALQ_PYRKO[18202014]
43:  Q08047
     1,4-alpha-glucan branching enzyme IIB, chloroplast precursor (Starch branching enzyme IIB) (Q-enzyme)
     gi|1169911|sp|Q08047|GLGB_MAIZE[1169911]
44:  Q9Z6V8
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17367290|sp|Q9Z6V8|GLGA_CHLPN[17367290]
45:  Q9WZZ7
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17367208|sp|Q9WZZ7|GLGA_THEMA[17367208]
46:  Q9RWS1
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17367076|sp|Q9RWS1|GLGA_DEIRA[17367076]
47:  Q9RNH6
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17367070|sp|Q9RNH6|GLGA_RHOSH[17367070]
48:  Q9PLC3
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17367025|sp|Q9PLC3|GLGA_CHLMU[17367025]
49:  Q9KRB6
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366937|sp|Q9KRB6|GLGA_VIBCH[17366937]
50:  Q9KDX6
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366934|sp|Q9KDX6|GLGA_BACHD[17366934]
51:  Q9I1V0
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366864|sp|Q9I1V0|GLGA_PSEAE[17366864]
52:  Q9EUT5
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366749|sp|Q9EUT5|GLGA_RHITR[17366749]
53:  Q9CN91
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366721|sp|Q9CN91|GLGA_PASMU[17366721]
54:  Q9CHM9
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366711|sp|Q9CHM9|GLGA_LACLA[17366711]
55:  Q985P2
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366611|sp|Q985P2|GLGA_RHILO[17366611]
56:  Q97QS5
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366608|sp|Q97QS5|GLGA_STRPN[17366608]
57:  Q97GX6
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366595|sp|Q97GX6|GLGA_CLOAB[17366595]
58:  P72623
     Probable glycogen synthase 2 (Starch [bacterial glycogen] synthase 2)
     gi|17366372|sp|P72623|GLG2_SYNY3[17366372]
59:  P58395
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366350|sp|P58395|GLGA_THECA[17366350]
60:  P58394
     Glycogen synthase 2 (Starch [bacterial glycogen] synthase 2)
     gi|17366347|sp|P58394|GLG2_RHIME[17366347]
61:  P58393
     Glycogen synthase 1 (Starch [bacterial glycogen] synthase 1)
     gi|17366344|sp|P58393|GLG1_RHIME[17366344]
62:  O84804
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366221|sp|O84804|GLGA_CHLTR[17366221]
63:  O66935
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|17366197|sp|O66935|GLGA_AQUAE[17366197]
64:  P35573
     Glycogen debranching enzyme (Glycogen debrancher) [Includes: 4-alpha-glucanotransferase (Oligo-1,4-1,4-glucantransferase); Amylo-alpha-1,6-
     glucosidase (Amylo-1,6-glucosidase) (Dextrin 6-alpha-D-glucosidase)]
     gi|8928542|sp|P35573|GDE_HUMAN[8928542]
65:  Q59001
     Probable glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|2842612|sp|Q59001|GLGA_METJA[2842612]
66:  P74521
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|2829618|sp|P74521|GLGA_SYNY3[2829618]
67:  O08328
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|2811062|sp|O08328|GLGA_BACST[2811062]
68:  P45179
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|1169909|sp|P45179|GLGA_HAEIN[1169909]
69:  P08323
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|1169908|sp|P08323|GLGA_ECOLI[1169908]
70:  P39125
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|729578|sp|P39125|GLGA_BACSU[729578]
71:  P39670
     Glycogen synthase (Starch [bacterial glycogen] synthase)
     gi|729577|sp|P39670|GLGA_AGRTU[729577]
72:  P35574
     Glycogen debranching enzyme (Glycogen debrancher) [Includes: 4-alpha-glucanotransferase (Oligo-1,4-1,4-glucantransferase); Amylo-alpha-1,6-
     glucosidase (Amylo-1,6-glucosidase) (Dextrin 6-alpha-D-glucosidase)]
     gi|544379|sp|P35574|GDE_RABIT[544379]
73:  P05416
     Glycogen synthase (Starch [bacterial glycogen] synthase) (Fragment)
     gi|121295|sp|P05416|GLGA_SALTY[121295]
74:  1G5AA
     Chain A, Amylosucrase From Neisseria Polysaccharea
     gi|16974797|pdb|1G5A|A[16974797]
75:  B26206
     alpha-1,4-glucan-protein synthase (UDP-forming) (EC 2.4.1.112) - rabbit (fragment)
     gi|89923|pir||B26206[89923]
76:  P30538
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (GLYCOGEN BRANCHING ENZYME)
     gi|232168|sp|P30538|GLGB_BACST[232168]
77:  Q9UUL4
     CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK12
     gi|11386953|sp|Q9UUL4|MOKC_SCHPO[11386953]
78:  CAC46426
     PHOSPHOGLYCEROL TRANSFERASE, CYCLIC BETA-1,2-GLUCAN MODIFICATION PROTEIN [Sinorhizobium meliloti]
     gi|15074781|emb|CAC46426.1|[15074781]
79:  Q09854
     CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK11
     gi|12644399|sp|Q09854|MOKB_SCHPO[12644399]
80:  P13834
     GLYCOGEN [STARCH] SYNTHASE, MUSCLE
     gi|12644124|sp|P13834|GYS1_RABIT[12644124]
81:  P10249
     SUCROSE PHOSPHORYLASE (SUCROSE GLUCOSYLTRANSFERASE) (GLUCOSYLTRANSFERASE-A) (GTF-A)
     gi|121726|sp|P10249|SUCP_STRMU[121726]
82:  Q9Y719
     CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK13
     gi|12643961|sp|Q9Y719|MOKD_SCHPO[12643961]
83:  Q9USK8
     CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK1
     gi|12643908|sp|Q9USK8|MOK1_SCHPO[12643908]
84:  Q9Z8L2
     4-ALPHA-GLUCANOTRANSFERASE (AMYLOMALTASE) (DISPROPORTIONATING ENZYME) (D-ENZYME)
     gi|6225648|sp|Q9Z8L2|MALQ_CHLPN[6225648]
85:  S41686
     geranylgeranyltransferase type I (EC 2.5.1.-) beta chain - fission yeast (Schizosaccharomyces pombe)
     gi|542213|pir||S41686[542213]
86:  O86956
     4-ALPHA-GLUCANOTRANSFERASE (AMYLOMALTASE) (DISPROPORTIONATING ENZYME) (D-ENZYME)
     gi|6225664|sp|O86956|MGTA_THENE[6225664]
87:  Q59266
     4-ALPHA-GLUCANOTRANSFERASE (AMYLOMALTASE) (DISPROPORTIONATING ENZYME) (D-ENZYME)
     gi|6225651|sp|Q59266|MALQ_CLOBU[6225651]
88:  O34022
     4-ALPHA-GLUCANOTRANSFERASE (AMYLOMALTASE) (DISPROPORTIONATING ENZYME) (D-ENZYME)
     gi|6225649|sp|O34022|MALQ_CHLPS[6225649]
89:  AAD37783
     glucan synthase [Paracoccidioides brasiliensis]
     gi|5007025|gb|AAD37783.1|AF148715_1[5007025]
90:  NQECA
     1,4-alpha-glucan branching enzyme (EC 2.4.1.18) - Escherichia coli
     gi|66573|pir||NQECA[66573]
91:  P49331
     GLUCOSYLTRANSFERASE-S PRECURSOR (GTF-S) (DEXTRANSUCRASE) (SUCROSE 6-GLUCOSYLTRANSFERASE)
     gi|6166192|sp|P49331|GTFD_STRMU[6166192]
92:  P08987
     GLUCOSYLTRANSFERASE-I PRECURSOR (GTF-I) (DEXTRANSUCRASE) (SUCROSE 6-GLUCOSYLTRANSFERASE)
     gi|6166191|sp|P08987|GTFB_STRMU[6166191]
93:  P13470
     GLUCOSYLTRANSFERASE-SI PRECURSOR (GTF-SI) (DEXTRANSUCRASE) (SUCROSE 6-GLUCOSYLTRANSFERASE)
     gi|121728|sp|P13470|GTFC_STRMU[121728]
94:  P53537
     ALPHA-GLUCAN PHOSPHORYLASE, H ISOZYME (STARCH PHOSPHORYLASE H)
     gi|1730560|sp|P53537|PHSH_VICFA[1730560]
95:  P32811
     ALPHA-GLUCAN PHOSPHORYLASE, H ISOZYME (STARCH PHOSPHORYLASE H)
     gi|417488|sp|P32811|PHSH_SOLTU[417488]
96:  P52979
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (GLYCOGEN BRANCHING ENZYME)
     gi|3915711|sp|P52979|GLGB_AGRTU[3915711]
97:  Q04952
     PUTATIVE 1,3-BETA-GLUCAN SYNTHASE COMPONENT (1,3-BETA-D-GLUCAN-UDP GLUCOSYLTRANSFERASE)
     gi|2498415|sp|Q04952|GLS3_YEAST[2498415]
98:  P40989
     1,3-BETA-GLUCAN SYNTHASE COMPONENT GLS2 (1,3-BETA-D-GLUCAN-UDP GLUCOSYLTRANSFERASE)
     gi|1707982|sp|P40989|GLS2_YEAST[1707982]
99:  P52980
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (GLYCOGEN BRANCHING ENZYME)
     gi|1707935|sp|P52980|GLGB_STRAU[1707935]
100: P38631
     1,3-BETA-GLUCAN SYNTHASE COMPONENT GLS1 (1,3-BETA-D-GLUCAN-UDP GLUCOSYLTRANSFERASE) (CND1 PROTEIN)
     (CWN53 PROTEIN) (FKS1 PROTEIN) (PAPULACANDIN B SENSITIVITY PROTEIN 1)
     gi|1346146|sp|P38631|GLS1_YEAST[1346146]
101: P32775
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (GLYCOGEN BRANCHING ENZYME)
     gi|729580|sp|P32775|GLGB_YEAST[729580]
102: Q01401
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (STARCH BRANCHING ENZYME) (Q-ENZYME)
     gi|399544|sp|Q01401|GLGB_ORYSA[399544]
103: P30539
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (GLYCOGEN BRANCHING ENZYME)
     gi|232169|sp|P30539|GLGB_BUTFI[232169]
104: P30537
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (GLYCOGEN BRANCHING ENZYME)
     gi|232167|sp|P30537|GLGB_BACCL[232167]
105: P29336
     GLUCOSYLTRANSFERASE-S PRECURSOR (GTF-S) (DEXTRANSUCRASE) (SUCROSE 6-GLUCOSYLTRANSFERASE)
     gi|121729|sp|P29336|GTFS_STRDO[121729]
106: P27470
     GLUCOSYLTRANSFERASE-I PRECURSOR (GTF-I) (DEXTRANSUCRASE) (SUCROSE 6-GLUCOSYLTRANSFERASE)
     gi|121725|sp|P27470|GTF2_STRDO[121725]
107: P11001
     GLUCOSYLTRANSFERASE-I PRECURSOR (GTF-I) (DEXTRANSUCRASE) (SUCROSE 6-GLUCOSYLTRANSFERASE)
     gi|121724|sp|P11001|GTF1_STRDO[121724]
108: P16954
     1,4-ALPHA-GLUCAN BRANCHING ENZYME (GLYCOGEN BRANCHING ENZYME)
     gi|121297|sp|P16954|GLGB_SYNP7[121297]
109: AAB41531
     cyclic beta-1,2-glucan modification protein; phosphoglycerol transferase [Sinorhizobium meliloti]
     gi|4733934|gb|AAB41531.2|[4733934]
110: AAC62210
     beta-(1-3)-glucosyl transferase [Bradyrhizobium japonicum]
     gi|3687658|gb|AAC62210.1|[3687658]
111: AAC61753
     beta-1,3 glucan transferase Bg|2p [Pichia jadinii]
     gi|3661545|gb|AAC61753.1|[3661545]
112: AAB46846
     alpha-1,4-glucan orthophosphate glycosyl transferase; myophosphorylase; glycogen phosphorylase isozyme [Bos taurus]
     gi|1836054|gb|AAB46846.1|[1836054]
113: AAB03100
     starch branching enzyme class II [Arabidopsis thaliana]
     gi|726490|gb|AAB03100.1|[726490]
114: AAB03099
     starch branching enzyme class II [Arabidopsis thaliana]
     gi|619939|gb|AAB03099.1|[619939]
115: CAA78143
     dimethylallyltransferase [Schizosaccharomyces pombe]
     gi|396477|emb|CAA78143.1|[396477]
116: AAA34632
     1,4-glucan-6-(1,4-glucano)-transferase [Saccharomyces cerevisiae]
     gi|171569|gb|AAA34632.1|[171569]
117: AAA23872
     branching enzyme (EC 2.4.1.18) [Escherichia coli]
     gi|146142|gb|AAA23872.1|[146142]
118: AAA21151
     beta-1,3 glucan transferase [Candida albicans]
     gi|532776|gb|AAA21151.1|[532776]

Claims

1. An isolated DNA molecule encoding a fusion protein consisting of four different functional domains selected from the group consisting of GLASS, LINKR, GLYTR, and CTEND which are operably linked to one another.

2. An isolated DNA molecule in claim 1, in which the GLASS domain comprises a GBSS GLASS.

3. The isolated DNA molecule in claim 2 wherein the GBSS GLASS comprises a GLASS of SEQ ID NO: 1

4. An isolated DNA molecule in claim 1, in which the GLASS domain comprises a SSI GLASS.

5. The isolated DNA molecule of claim 4 wherein the SSI GLASS comprises a GLASS of SEQ ID NO: 2

6. An isolated DNA molecule in claim 1, in which the GLASS domain comprises a SSII GLASS.

7. The isolated DNA molecule of claim 6 wherein the SSII GLASS comprises a GLASS of SEQ ID NOs. 3 and 4

8. An isolated DNA molecule in claim 1, in which the GLASS domain comprises a SSIII GLASS.

9. The isolated DNA molecule of claim 8 wherein the SSIII GLASS comprises a GLASS of SEQ ID NO:5

10. The isolated DNA molecule of claim 2, wherein said GBSS GLASS is a GLASS of a glucan producing organism.

11. The isolated DNA molecule of claim 4, wherein said SSI-GLASS is a GLASS domain of a glucan producing organism.

12. The isolated DNA molecule of claim 6, wherein said SSII-GLASS is a GLASS of a glucan producing organism.

13. The isolated DNA molecule of claim 8, wherein said SSIII-GLASS is a GLASS of a glucan producing organism.

14. The isolated DNA molecule of claim 2 wherein said GBSS-GLASS domain is at least 80% identical to a GLASS peptide of a glucan producing organism.

15. The isolated DNA molecule of claim 4 wherein said SSI-GLASS domain is at least 80% identical to a GLASS peptide of a glucan producing organism.

16. The isolated DNA molecule of claim 6 wherein said SSII-GLASS domain is at least 80% identical to a GLASS peptide of a glucan producing organism.

17. The isolated DNA molecule of claim 8 wherein said SSIII-GLASS domain is at least 80% identical to a GLASS peptide of a glucan producing organism.

18. An isolated DNA molecule of any of the claims in 2, 4, 6 or 8, in which the LINKR domain is a GBSS-LINKR, SSI-LINKR, SSII-LINKR or SSIII-LINKR.

19. The isolated DNA molecule of claim 18 wherein said LINKR comprises a LINKR sequence selected from SEQ ID NOs:121-171; 336-386;527-577; 733-783; and 983-1033.

20. An isolated DNA molecule of any of claims 2, 4, 6 or 8, in which the GLYTR domain is a GBSS-GLYTR, SSI-GLYTR, SSII-GLYTR or SSIII-GLYTR.

21. An isolated DNA molecule of claim 18, in which the GLYTR is a GBSS-GLYTR, SSI-GLYTR, SSII-GLYTR or SSfi-GLYTR.

22. The isolated DNA molecule of claim 20 wherein said GLYTR comprises a GLYTR sequence selected from SEQ ID NOs:1136, 1137, 1138, 1139, and 1140.

23. The isolated DNA molecule of claim 21 wherein said GLYTR comprises a GLYTR sequence selected from SEQ ID NOs: 172-222; 387-437; 578-628; 784-834; and 1034-1084.

24. An isolated DNA molecule of claims 2, 4, 6 or 8, in which the CTEND domain is a GBSS-CTEND, SSI-CTEND, SSII-CTEND or SSIII-CTEND.

25. An isolated DNA molecule of claim 18, in which the CTEND domain is a GBSS-CTEND, SSI-CTEND, SSII-CTEND or SSIII-CTEND.

26. An isolated DNA molecule of claim 20, in which the CTEND domain is a GBSS-CTEND, SSI-CTEND, SSII-CTEND or SSIII-CTEND.

27. The isolated DNA molecule of claim 24 wherein said CTEND sequence comprises a CTEND sequence selected from SEQ ID NOs: 1146, 1147, 1148, 1148, 1149 and 1150

28. The isolated DNA molecule of claim 25 wherein said CTEND sequence comprises a CTEND sequence selected from SEQ ID NOs:223-266; 438-461; 629-676; 835-882; and 1085-1135.

29. The isolated DNA molecule of claim 26 wherein said CTEND sequence comprises a CTEND sequence selected from SEQ ID NOs: 223-266; 438-461; 629-676; 835-882; and 1085-1135.

30. An isolated DNA molecule encoding a fusion peptide comprising a GBSS GLASS domain operably linked to a LINKR and a catalytic domain from a functional protein that synthesizes an α-1,4 glucan or an α-1,3 glucan, or an α-1,6 glucan, said fusion peptide being capable of modifying the glucan structure of a starch producing organism when starch is produced by said organism or part thereof in the presence of said fusion peptide.

31. The DNA molecule of claim 30 wherein said fusion peptide comprises a GLASS and/or a LINKR sequence of SEQ ID NOs:75-120; 284-335, 475-526; 682-732; 933-982 and/or 121-171, 336-386, 527-577, 733-783, and 983-1033.

32. An isolated DNA molecule encoding a polypeptide with glucan association properties of a maize GBSS enzyme capable of modification of starch metabolism in a plant or plant cell, said DNA comprising a molecule selected from the group consisting of: (a) a DNA molecule encoding a protein domain having the amino acid SEQ ID No.1 (b) a DNA molecule comprising a corresponding nucleotide sequence from SEQ ID No.1141 (c) a DNA molecule comprising a nucleotide sequence differing from the sequence of the DNA molecules of (a) or (b) due to the degeneracy of the genetic code, (d) a DNA molecule comprising a DNA sequence which hybridizes to any one of the DNA molecules of (a), (b) or (c) or fragment thereof, and which is equal to or more than 80% homologous or identical to the DNA molecule of (a), (b), or (c), or fragment thereof, wherein said DNA sequence encodes a polypeptide with Glucan Association Domain (Domain A) of a GBSS enzyme.

33. An isolated DNA molecule encoding a polypeptide with a glycosyl transferase function of a soluble or granule bound maize SS enzymes capable of modifying starch metabolism in a plant or plant cell, said DNA molecule being selected from the group consisting of: (a) a DNA molecule encoding a protein domain comprising an amino acid of SEQ ID NOs:1, 2, 3, 4, and 149. (b) a DNA molecule comprising the corresponding nucleotide sequence of SEQ ID NOs: 1141, 11142, 1143, 1144, and 1145, (c) a DNA molecule comprising a nucleotide sequence differing from the sequence of the DNA molecules of (a) or (b) due to the degeneracy of the genetic code, (d) a DNA molecule comprising a DNA sequence which hybridizes to any one of the DNA molecules of (a), (b) or (c), or fragment thereof, and which is equal to or more than 80% homologous or identical to the DNA molecule of (a), (b), or (c), or fragment thereof, wherein said DNA sequence encodes a polypeptide with a glycosyl transferase domain of a SS enzyme.

34. A recombinant DNA molecule comprising a DNA molecule of any of the above claims comprising a maize GBSS nucleotide coding region encoding for an amino acid sequence of SEQ. ID NOs. 101-146 fused with a corresponding coding region of a maize SS enzyme that encode for an amino acid sequence of SEQ. ID NOs: 35-74; 121-171; 172-222; 223-266; 268-283; 284-335; 336-386; 387-437; 438-461; 463-474; 475-526; 527-577; 578-628; 629-676; 678-681; 682-732; 733-834; 835-882; 884-932; 933-982; 1034-1084; and 1085-1135.

35. A recombinant DNA molecule comprising a DNA molecule of any of the above claims comprising a GLYTR, LINKR or CTEND domain DNA sequence selected from any one of SEQ ID NOs: 172-222; 387-437; 578-628; 784-834; 1034-1084, 121-171; 336-386; 527-577; 733-783; 983-1033 or 223-266; 438-461; 629-676; 835-882; 1085-1135 operably linked in any order with a corresponding DNA sequence that encodes for a glucan association domain from any one of SEQ ID NOs: 75-120; 284-335; 475-526; 682-732; 933-982.

36. A recombinant DNA molecule comprising a DNA molecule of any of the above claims comprising a DNA sequence differing from the sequence of any of the DNA molecules of SEQ ID NOs: 34-1150 due to the degeneracy of the genetic code, and/or protein or polypeptide originating from a different source, such as a plant species other than plant species such as maize, bacteria (e.g. E. Coli), Yeast, algae (Chlamydomonas), or fungus.

37. A recombinant DNA molecule comprising a DNA molecule of any of the above claims comprising a wherein the DNA sequence is selected from the group consisting of a coding region of a glucan association domain of SEQ ID NOs:75-120; 284-335; 475-526; 682-732; and 933-982 fused with a coding region of any glucan transferases listed in table XXXVII.

38. Method of expressing a starch synthase fusion proteins or polypeptides in a plant, in which the starch synthase protein or polypeptide domains are expressed as a fusion with a glucan association domain of granule bound starch synthase.

39 A method according to any one of the preceding claims, in which the protein or polypeptide is heterologous with respect to the plant in which the fusion is expressed.

40. Method according to any one of the preceding claims, comprising the steps of: providing a genetic construct comprising at least one nucleotide sequence encoding the desired protein domain or polypeptide domain combined with at least one nucleotide sequence encoding a glucan association domain of GBSS, so that the construct encodes a fusion of the desired protein/polypeptide and at least one glucan association domain; transforming a plant with said genetic construct; expressing said genetic construct in the plant.

41. Method according to any one of the preceding claims in which the protein or polypeptide or recombinant protein or recombinant polypeptide is an enzyme.

42. Method according to claim 41, in which the enzyme is an enzyme that can interact and associate with starch or starch granules, or facilitate or be entrapped in starch or starch granules, and is capable of at least one of modifying, increasing, decreasing, altering or influencing starch structure or starch synthesis.

43. A vector comprising a DNA molecule according to any of the preceding claims.

44. A vector according to claim 43, wherein the DNA molecule is linked in sense orientation to DNA elements ensuring transcription of a translatable RNA in a prokaryotic or an eukaryotic cell.

45. A host cell comprising a vector according to claim 43.

46. A plant cell comprising a DNA molecule according to any one of the preceding claims linked to a heterologous promoter.

47. A plant comprising a plant cell according to claim 46.

48. The plant of claim 47, which is a cereal, such as maize, rice, wheat, barley, oats, or a root crop, such as potato, sweet potato, cassaya, yam, taro, or other starch producing plant, such as peas or banana.

49. A plant according to claim 47 wherein said plant contains or produces starch or starch granules in at least one of its parts, including its seeds, leaves, roots (tubers), tubers, stems, stalks, fruits, grains or flowers.

50. A plant according to claim 49 wherein said elements include a homologous or heterologous promoter specific for expression of said DNA molecule in said at least one of its parts.

51. A seed from the plant of claim 49, capable of expressing said recombinant molecule.

52. A modified starch derived from cells of a plant of any of the preceding claims.

53. Food or feed comprising a modified starch of claim 52.