Claims
- 1. A chimeric protein comprising a leukotoxin polypeptide fused to a multimer having more than one selected GnRH polypeptide, whereby said leukotoxin portion of said chimeric protein acts to increase the immunogenicity of said GnRH multimer.
- 2. The chimeric protein of claim 1 wherein said leukotoxin polypeptide lacks leukotoxic activity.
- 3. The chimeric protein of claim 2 wherein said leukotoxin is LKT 352.
- 4. The chimeric protein of claim 2 wherein said leukotoxin is LKT 111.
- 5. The chimeric protein of claim 1 wherein said GnRH multimer comprises a molecule according to the general formula GnRH-X-GnRH wherein X is selected from the group consisting of a peptide linkage, an amino acid spacer group, a leukotoxin polypeptide and �GnRH!.sub.n where n is greater than or equal to 1, and further wherein GnRH comprises any GnRH polypeptide.
- 6. The chimeric protein of claim 5 wherein X comprises an amino acid spacer group including at least one helper T-cell epitope.
- 7. The chimeric protein of claim 1 wherein said chimeric protein comprises the amino acid sequence depicted in FIGS. 5A-5h, SEQ ID NOS:7-8.
- 8. The chimeric protein of claim 1 wherein said chimeric protein comprises the amino acid sequence depicted in FIGS. 7A-7E, SEQ ID NOS:9-10.
- 9. A vaccine composition comprising the chimeric protein of claim 1 and a pharmaceutically acceptable vehicle.
- 10. A vaccine composition comprising the chimeric protein of claim 2 and a pharmaceutically acceptable vehicle.
- 11. A vaccine composition comprising the chimeric protein of claim 5 and a pharmaceutically acceptable vehicle.
- 12. A vaccine composition comprising the chimeric protein of claim 7 and a pharmaceutically acceptable vehicle.
- 13. A vaccine composition comprising the chimeric protein of claim 8 and a pharmaceutically acceptable vehicle.
- 14. A method for presenting a selected GnRH multimer to a subject comprising administering to said subject an effective amount of a vaccine composition according to claim 9.
- 15. A method for presenting a selected GnRH multimer to a subject comprising administering to said subject an effective amount of a vaccine composition according to claim 10.
- 16. A method for presenting a selected GnRH multimer to a subject comprising administering to said subject an effective amount of a vaccine composition according to claim 11.
- 17. A method for presenting a selected GnRH multimer to a subject comprising administering to said subject an effective amount of a vaccine composition according to claim 12.
- 18. A method for presenting a selected GnRH multimer to a subject comprising administering to said subject an effective amount of a vaccine composition according to claim 13.
- 19. A chimeric protein comprising a leukotoxin polypeptide fused to a multimer having eight selected GnRH polypeptides, wherein the C-terminus of the leukotoxin polypeptide is fused to the N-terminus of the multimer.
- 20. The chimeric protein of claim 19, wherein the leukotoxin polypeptide comprises the 52 kD LKT 111 carrier polypeptide.
- 21. A chimeric protein comprising a leukotoxin polypeptide fused to a multimer having eight selected GnRH polypeptides, wherein the C-terminus of the multimer is fused to the N-terminus of the leukotoxin polypeptide.
- 22. The chimeric protein of claim 21, wherein the leukotoxin polypeptide comprises the 52 kD LKT 111 carrier polypeptide.
- 23. A vaccine composition comprising the chimeric protein of claim 19 and a pharmaceutically acceptable vehicle.
- 24. A vaccine composition comprising the chimeric protein of claim 20 and a pharmaceutically acceptable vehicle.
- 25. A vaccine composition comprising the chimeric protein of claim 21 and a pharmaceutically acceptable vehicle.
- 26. A vaccine composition comprising the chimeric protein of claim 22 and a pharmaceutically acceptable vehicle.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of U.S. patent application Ser. No. 07/960,932, filed 14 Oct. 1992 and issued as U.S. Pat. No. 5,422,110, which is a continuation-in-part of U.S. patent application Ser. No. 07/779,171, filed 16 Oct. 1991 now abandoned, which applications are incorporated by reference herein in their entireties and from which priority is claimed pursuant to 35 USC .sctn. 120.
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Continuation in Parts (2)
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Number |
Date |
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Parent |
960932 |
Oct 1992 |
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Parent |
779171 |
Oct 1991 |
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