Patent Application
20240343704
The present application relates to halo-substituted amino pyridine compounds, to processes for their preparation, to compositions comprising them, and to their use in therapy. More particularly, it relates to halo substituted amino pyridine compounds useful in the treatment of diseases, disorders or conditions treatable by inhibiting HPK1.
Tumors are genetically heterogeneous and have evolved mechanisms to hijack cellular growth and regulatory pathways, which makes it unlikely a single therapy will have a significant impact on patient survival. For this reason, immunotherapy has become an important paradigm in the treatment of some types of cancers. Immune effector cells such as T-cells and B-cells can suppress the proliferation of cancer cells by targeting abnormal, tumor-expressed antigens. For example, recent clinical testing of novel immunotherapy strategies (e.g. anti-PD1 and anti-PDL1) has demonstrated unprecedented and durable survival benefit even in advanced patients suffering from metastatic cancers. However, the overall excitement for this therapeutic approach is tempered by the observation that these responses to agents targeting the PD-1 axis are limited to a minority of cancer patients. Hence, in order to broaden the response rates in cancer patients, there is an urgent need to build on the tremendous promise of immunotherapy to more rapidly test rational combinations of small molecules with immuno-therapeutics. One such approach is the combination of a small molecule hematopoietic progenitor kinase 1 (HPK1) inhibitor with current anti-PD1/PDL1 immunotherapies. An HPK1 inhibitor should potentiate anti-tumor immune responses by stimulating T-cell proliferation and triggering tumor cell senescence and tumor clearance by T cells.
The hematopoietic progenitor kinase 1 (HPK1, MAP4K1), is a T-cell receptor (TCR)-proximal kinase involved in the regulation of proliferation and survival of primary T cells [Nat Immunol. 2007; 8(1):84-91.] HPK1 is exclusively expressed in hematopoietic tissues and activates the c-Jun N-terminal kinase (JNK) and the NF-kB pathways [7]. Transient knockdown of HPK1 in T cells blocks activation of NF-kB [Crit Rev Oncol Hematol. 2008; 66(1):52-64]. Most strikingly, mice that received adoptive transfer of HPKI (−/−) T cells became resistant to lung tumor growth [Immunol Res. 2012; 54(1-3):262-5]. HPK1 has an N-terminal kinase domain and a C-terminal citron homology domain. Antigen receptor cross-linking leads to activation of HPK1 in T and B cells resulting in HPK1 relocation to the plasma membrane, autophosphorylation and transphosphorylation by protein kinase D1 (PKD1). Subsequent transphosphorylation by PKD1 and auto-phosphorylation within the kinase domain result in full activation of HPK1, which then regulates different cellular responses including apoptosis, activation-induced cell death and autoimmunity. HPK1 mediates negative regulation of the immune response via phosphorylation of SLP-76 (S376). Mutation of lysine-46 to methionine (designated HPK1-M46) in the ATP-binding site of the kinase domain abolishes catalytic activation of HPK1 resulting in a kinase-dead version of the full length kinase [Genes Dev. 1996; 10 (18):2251-64]. It has been reported that HPK1 inhibition in HPKI kinase-dead knock-in mice, when treated with anti-PD-1 or anti-PDL1 antibodies demonstrate enhanced efficacy in colon cancer models relative to anti-PD-1 or anti-PDL1 treatment alone (Cell Reports 2018, 25, 80-94, and PCT Patent Application Publication Nos. WO2016/205942 and WO2016/090300). Combining or sequencing immunotherapies that target distinct immune pathways is therefore a rational strategy to increase the magnitude of the antitumor immune response over that generated with a single agent.
HPK1 plays significant roles in regulating lymphocyte receptor signaling and function. Moreover, the restricted expression of HPK1 in hematopoietic cells and the roles of HPK1 in immune cells suggest that HPK1 would be an ideal drug target for enhancing antitumor immunity. Furthermore, data from preclinical studies suggest that gene-targeted disruption of HPK1 can promote the proliferation, survival, and function of various immune cells [e.g., T cells, NK cells, and dendritic cells (DC)], and synergistically inhibit tumor growth with anti-PD-1/PDL-1 mAb. Support for this strong rationale was evident by some reports in the literature that HPK1 kinase-dead knock-in mouse bearing colorectal tumors (MC38) showed significant growth arrest treated with an anti-PD1 or anti-PDL1 antibody (PCT Patent Application Publication No. WO2016/090300). Thus, combining a small molecule that inhibits HPK1 with another immunotherapy would appear to be a rational and more effective approach toward treating cancers.
Inhibiting kinases such as HPK1 therefore represents promising targets for immunooncology due to their role in limiting T-cell activation. At the same time, in the pursuit of these targets it is desirable that there be selectivity against other kinases that are involved in a robust T cell activation. An example of such a kinase includes, but is not limited to Lck (Sawasdikosol, et. al. Structure 27, 2019, 1-3).
Current cancer immunotherapy strategies seek to reverse immune tolerance either by modulating T cell co-receptor signals or boosting the recognition of tumor-associated antigens by using native biomolecules or mAbs. Selective HPK1 inhibitors, combined with other immuno-modulating agents should amplify the anti-tumor activity of immune cells. The present application discloses novel compounds that have such activity.
Accordingly, the application includes a compound of Formula (I), or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof,
The present application also includes a composition comprising one or more compounds of the application and a carrier. In an embodiment, the composition is a pharmaceutical composition comprising one or more compounds of the application and a pharmaceutically acceptable carrier.
In an embodiment, the compounds of the application are used as medicaments. Accordingly, the application also includes a compound of the application for use as a medicament.
The compounds of the application have been shown to inhibit HPK1. Therefore the compounds of the application are useful for treating diseases, disorders or conditions that are treatable by inhibiting HPK1 activity. Accordingly, the present application also includes a method of treating a disease, disorder or condition that is treatable by inhibiting HPK1, comprising administering a therapeutically effective amount of one or more compounds of the application to a subject in need thereof.
The present application also includes a use of one or more compounds of the application for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1, as well as a use of one or more compounds of the application for the preparation of a medicament for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1. The application further includes one or more compounds of the application for use in treating a disease, disorder or condition that is treatable by inhibiting HPK1.
In a further embodiment, the disease, disorder or condition that is treatable by inhibiting HPK1 is cancer and the one or more compounds of the application are administered in combination with one or more additional cancer treatments. In another embodiment, the additional cancer treatment is selected from radiotherapy, chemotherapy, targeted therapies such as antibody therapies and small molecule therapies such as tyrosine-kinase inhibitors, immunotherapy, hormonal therapy and anti-angiogenic therapies. In another embodiment, the additional cancer treatment is selected from an antibody that binds to PD-1 or PD-1.
The application additionally provides a process for the preparation of compounds of the application. General and specific processes are discussed in more detail and set forth in the Examples below.
Other features and advantages of the present application will become apparent from the following detailed description. It should be understood, however, that the detailed description and the specific examples, while indicating embodiments of the application, are given by way of illustration only and the scope of the claims should not be limited by these embodiments, but should be given the broadest interpretation consistent with the description as a whole.
Unless otherwise indicated, the definitions and embodiments described in this and other sections are intended to be applicable to all embodiments and aspects of the present application herein described for which they are suitable as would be understood by a person skilled in the art.
All features disclosed in the specification, including the claims, abstract, and drawings, and all the steps in any method or process disclosed, may be combined in any combination, except combinations where at least some of such features and/or steps are mutually exclusive. Each feature disclosed in the specification, including the claims, abstract, and drawings, can be replaced by alternative features serving the same, equivalent, or similar purpose, unless expressly stated otherwise.
As used in this application and claim(s), the words “comprising” (and any form of comprising, such as “comprise” and “comprises”), “having” (and any form of having, such as “have” and “has”), “including” (and any form of including, such as “include” and “includes”) or “containing” (and any form of containing, such as “contain” and “contains”), are inclusive or open-ended and do not exclude additional, unrecited elements or process steps.
The term “consisting” and its derivatives as used herein are intended to be closed terms that specify the presence of the stated features, elements, components, groups, integers, and/or steps, and also exclude the presence of other unstated features, elements, components, groups, integers and/or steps.
The term “consisting essentially of”, as used herein, is intended to specify the presence of the stated features, elements, components, groups, integers, and/or steps as well as those that do not materially affect the basic and novel characteristic(s) of these features, elements, components, groups, integers, and/or steps.
The terms “about”, “substantially” and “approximately” as used herein mean a reasonable amount of deviation of the modified term such that the end result is not significantly changed. These terms of degree should be construed as including a deviation of at least ±5% of the modified term if this deviation would not negate the meaning of the word it modifies or unless the context suggests otherwise to a person skilled in the art.
As used in the present application, the singular forms “a”, “an” and “the” include plural references unless the content clearly dictates otherwise. For example, an embodiment including “a compound” should be understood to present certain aspects with one compound, or two or more additional compounds.
In embodiments comprising an “additional” or “second” component or effect, such as an additional or second compound, the second compound as used herein is different from the other compounds or first compound. A “third” compound is different from the other, first, and second compounds, and further enumerated or “additional” compounds are similarly different.
The term “and/or” as used herein means that the listed items are present, or used, individually or in combination. In effect, this term means that “at least one of” or “one or more” of the listed items is used or present. The term “and/or” with respect to enantiomers, prodrugs, salts and/or solvates thereof means that the compounds of the application exist as individual enantiomers, prodrugs, salts and hydrates, as well as a combination of, for example, a salt of a solvate of a compound of the application.
The term “compound of the application” or “compound of the present application” and the like as used herein refers to a compound of Formula (I) or Formula I-A, I-B, I-C, I-D, I-E, I-F, I-G, I-H, I-J, I-K, I-L, I-M, I-N, I-O, I-P, I-Q, I-R, I-S, I-T and I-U or salts, solvates and/or prodrugs thereof.
The term “composition of the application” or “composition of the present application” and the like as used herein refers to a composition comprising one or more compounds of the application.
The term “suitable” as used herein means that the selection of the particular compound or conditions would depend on the specific synthetic manipulation to be performed, the identity of the molecule(s) to be transformed and/or the specific use for the compound, but the selection would be well within the skill of a person trained in the art.
The present description refers to a number of chemical terms and abbreviations used by those skilled in the art. Nevertheless, definitions of selected terms are provided for clarity and consistency.
The term “protecting group” or “PG” and the like as used herein refers to a chemical moiety which protects or masks a reactive portion of a molecule to prevent side reactions in those reactive portions of the molecule, while manipulating or reacting a different portion of the molecule. After the manipulation or reaction is complete, the protecting group is removed under conditions that do not degrade or decompose the remaining portions of the molecule. The selection of a suitable protecting group can be made by a person skilled in the art. Many conventional protecting groups are known in the art, for example as described in “Protective Groups in Organic Chemistry” McOmie, J. F. W. Ed., Plenum Press, 1973, in Greene, T. W. and Wuts, P. G. M., “Protective Groups in Organic Synthesis”, John Wiley & Sons, 3rd Edition, 1999 and in Kocienski, P. Protecting Groups, 3rd Edition, 2003, Georg Thieme Verlag (The Americas).
The term “inert organic solvent” as used herein refers to a solvent that is generally considered as non-reactive with the functional groups that are present in the compounds to be combined together in any given reaction so that it does not interfere with or inhibit the desired synthetic transformation. Organic solvents are typically non-polar and dissolve compounds that are non soluble in aqueous solutions.
The term “alkyl” as used herein, whether it is used alone or as part of another group, means straight or branched chain, saturated alkyl groups. The number of carbon atoms that are possible in the referenced alkyl group are indicated by the prefix “Cn1-n2”. For example, the term C1-10alkyl means an alkyl group having 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 carbon atoms. All alkyl groups are optionally fluoro-substituted unless otherwise indicated.
The term “alkylene”, whether it is used alone or as part of another group, means straight or branched chain, saturated alkylene group, that is, a saturated carbon chain that contains substituents on two of its ends. The number of carbon atoms that are possible in the referenced alkylene group are indicated by the prefix “Cn1-n2”. For example, the term C2-6alkylene means an alkylene group having 2, 3, 4, 5 or 6 carbon atoms. All alkylene groups are optionally fluoro-substituted unless otherwise indicated.
The term “alkenyl” as used herein, whether it is used alone or as part of another group, means straight or branched chain, unsaturated alkyl groups containing at least one double bond. The number of carbon atoms that are possible in the referenced alkylene group are indicated by the prefix “Cn1-n2”. For example, the term C2-6alkenyl means an alkenyl group having 2, 3, 4, 5 or 6 carbon atoms and at least one double bond. All alkenyl groups are optionally fluoro-substituted unless otherwise indicated.
The term “alkynyl” as used herein, whether it is used alone or as part of another group, means straight or branched chain, unsaturated alkynyl groups containing at least one triple bond. The number of carbon atoms that are possible in the referenced alkyl group are indicated by the prefix “Cn1-n2”. For example, the term C2-6alkynyl means an alkynyl group having 2, 3, 4, 5 or 6 carbon atoms. All alkynyl groups are optionally fluoro-substituted unless otherwise indicated.
The term “cycloalkyl,” as used herein, whether it is used alone or as part of another group, means a saturated carbocyclic group containing from 3 to 20 carbon atoms and one or more rings. The number of carbon atoms that are possible in the referenced cycloalkyl group are indicated by the numerical prefix “Cn1-n2”. For example, the term C3-10cycloalkyl means a cycloalkyl group having 3, 4, 5, 6, 7, 8, 9 or 10 carbon atoms. All cycloalkyl groups are optionally fluoro-substituted unless otherwise indicated.
The term “aryl” as used herein, whether it is used alone or as part of another group, refers to carbocyclic groups containing at least one aromatic ring and contains 6 to 20 carbon atoms. All aryl groups are optionally fluoro-substituted unless otherwise indicated.
The term “heterocycloalkyl” as used herein, whether it is used alone or as part of another group, refers to cyclic groups containing at least one non-aromatic ring containing from 3 to 20 atoms in which one or more of the atoms are a heteroatom selected from O, S and N and the remaining atoms are C. Heterocycloalkyl groups are either saturated or unsaturated (i.e. contain one or more double bonds). When a heterocycloalkyl group contains the prefix Cn1-n2 this prefix indicates the number of carbon atoms in the corresponding carbocyclic group, in which one or more, suitably 1 to 5, of the ring atoms is replaced with a heteroatom as selected from O, S and N and the remaining atoms are C. Heterocycloalkyl groups are optionally benzofused. The heteroatom may be substituted or oxidized where valency allows. All heterocycloalkyl groups are optionally fluoro-substituted unless otherwise indicated.
The term “heteroaryl” as used herein, whether it is used alone or as part of another group, refers to cyclic groups containing at least one heteroaromatic ring containing 5-20 atoms in which one or more of the atoms are a heteroatom selected from O, S and N and the remaining atoms are C. When a heteroaryl group contains the prefix Cn1-n2 this prefix indicates the number of carbon atoms in the corresponding carbocyclic group, in which one or more, suitably 1 to 5, of the ring atoms is replaced with a heteroatom as defined above. Heteroaryl groups are optionally benzofused. The heteroatom may be substituted or oxidized where valency allows. All heteroaryl groups are optionally fluoro-substituted unless otherwise indicated.
All cyclic groups, including aryl, heteroaryl, heterocycloalkyl, and cycloalkyl groups, contain one or more than one ring (i.e. are polycyclic). When a cyclic group contains more than one ring, the rings may be fused, bridged, and/or spirofused.
The term “benzofused” as used herein refers to a polycyclic group in which a benzene ring is fused with another ring.
A first ring being “fused” with a second ring means the first ring and the second ring share two adjacent atoms there between.
A first ring being “bridged” with a second ring means the first ring and the second ring share two non-adjacent atoms there between.
A first ring being “spirofused” with a second ring means the first ring and the second ring share one atom there between.
The term “fluoro-substituted” refers to the substitution of one or more, including all, available hydrogens in a referenced group with fluoro.
The terms “halo” or “halogen” as used herein, whether it is used alone or as part of another group, refers to a halogen atom and includes fluoro, chloro, bromo and iodo.
The term “available”, as in “available hydrogen atoms” or “available atoms” refers to atoms that would be known to a person skilled in the art to be capable of replacement by a substituent, such as a fluorine atom.
When a group is said to be substituted with multiple substituents, said substituents are independently selected therefore can be the same or different.
It is to be clear that all available hydrogen atoms in the compounds of the application, and all embodiments thereof, are optionally substituted with a fluorine atom unless otherwise indicated.
The term “cross-coupling” as used herein refers to chemical reactions in which two different starting materials, each of which is usually endowed with an activating group, are reacted together with the aid of a metal catalyst. The result is the loss of the two activating groups and the formation of a new covalent bond between the remaining fragments.
The term “cell” as used herein refers to a single cell or a plurality of cells and includes a cell either in a cell culture or in a subject.
The term “subject” as used herein includes all members of the animal kingdom including mammals, and suitably refers to humans. Thus the methods and uses of the present application are applicable to both human therapy and veterinary applications.
The term “pharmaceutically acceptable” means compatible with the treatment of subjects, for example humans.
The term “pharmaceutically acceptable carrier” means a non-toxic solvent, dispersant, excipient, adjuvant or other material which is mixed with the active ingredient in order to permit the formation of a pharmaceutical composition, i.e., a dosage form capable of administration to a subject.
The term “pharmaceutically acceptable salt” means either an acid addition salt or a base addition salt which is suitable for, or compatible with the treatment of subjects.
The term “solvate” as used herein means a compound, or a salt and/or prodrug of a compound, wherein molecules of a suitable solvent are incorporated in the crystal lattice. A suitable solvent is physiologically tolerable at the dosage administered.
The term “prodrug” as used herein means a compound, or salt and/or solvate of a compound, that, after administration, is converted into an active drug.
The term “treating” or “treatment” as used herein and as is well understood in the art, means an approach for obtaining beneficial or desired results, including clinical results. Beneficial or desired clinical results can include, but are not limited to alleviation or amelioration of one or more symptoms or conditions, diminishment of extent of disease, stabilized (i.e. not worsening) state of disease, preventing spread of disease, delay or slowing of disease progression, amelioration or palliation of the disease state, diminishment of the reoccurrence of disease, and remission (whether partial or total), whether detectable or undetectable. “Treating” and “treatment” can also mean prolonging survival as compared to expected survival if not receiving treatment. “Treating” and “treatment” as used herein also include prophylactic treatment. For example, a subject with early cancer can be treated to prevent progression, or alternatively a subject in remission can be treated with a compound or composition of the application to prevent recurrence. Treatment methods comprise administering to a subject a therapeutically effective amount of one or more of the compounds of the application and optionally consist of a single administration, or alternatively comprise a series of administrations.
“Palliating” a disease or disorder means that the extent and/or undesirable clinical manifestations of a disorder or a disease state are lessened and/or time course of the progression is slowed or lengthened, as compared to not treating the disorder.
The term “prevention” or “prophylaxis”, or synonym thereto, as used herein refers to a reduction in the risk or probability of a patient becoming afflicted with a disease, disorder or condition treatable by inhibiting HPK1, or manifesting a symptom associated with a disease, disorder or condition treatable by inhibition of HPK1.
As used herein, the term “effective amount” or “therapeutically effective amount” means an amount of a compound, or one or more compounds, of the application that is effective, at dosages and for periods of time necessary to achieve the desired result.
The expression “inhibiting HPK1” as used herein refers to inhibiting, blocking and/or disrupting HPK1 enzymatic activity in a cell, in particular a T-cell or B-cell. The inhibiting, blocking and/or disrupting causes a therapeutic effect in the cell.
By “inhibiting, blocking and/or disrupting” it is meant any detectable inhibition, block and/or disruption in the presence of a compound compared to otherwise the same conditions, except for in the absence in the compound.
The term “disease, disorder or condition treatable by inhibiting HPK1” means that the disease, disorder or condition to be treated is affected by, modulated by and/or has some biological basis, either direct or indirect, that includes HPK1 activity, in particular, increased HPK1 activity. These diseases respond favourably when HPK1 activity associated with the disease, disorder or condition is inhibited by one or more of the compounds or compositions of the application.
The term “HPK1” as used herein refers to the hematopoetic progenitor kinase 1.
The term “administered” as used herein means administration of a therapeutically effective amount of a compound, or one or more compounds, or a composition of the application to a cell either in cell culture or in a subject.
The term “neoplastic disorder” as used herein refers to a disease, disorder or condition characterized by cells that have the capacity for autonomous growth or replication, e.g., an abnormal state or condition characterized by proliferative cell growth. The term “neoplasm” as used herein refers to a mass of tissue resulting from the abnormal growth and/or division of cells in a subject having a neoplastic disorder. Neoplasms can be benign (such as uterine fibroids and melanocytic nevi), potentially malignant (such as carcinoma in situ) or malignant (i.e. cancer).
The term “cancer” as used herein refers to cellular-proliferative disease states.
As used herein, the term “effective amount” means an amount effective, at dosages and for periods of time, necessary to achieve a desired result.
The present application describes a novel class of halo-substituted heterocyclic HPK1 inhibitors.
Accordingly, the application includes a compound of Formula (I) or a pharmaceutically acceptable salt, solvate and/or prodrug thereof,
wherein:
The application also includes a compound of Formula (I) or a pharmaceutically acceptable salt, solvate and/or prodrug thereof,
wherein:
In all embodiments below it is to be understood that all available hydrogen atoms are optionally substituted with a fluorine atom. This has not been repeated throughout. Thus in each embodiment where a group is listed that comprises available hydrogen atoms, it is to be understood that all such atoms are optionally replaced with fluorine atoms, for example each recitation of C1-6alkyl is also a recitation of C1-6fluoroalkyl, unless stated otherwise.
In an embodiment, X1 is N.
In an embodiment, X1 is CR3.
In an embodiment, R3 is selected from H, F, Cl, OR5a, NR7R8, C1-4alkyleneNR7aR8a and C1-4alkyl.
In an embodiment, R3 is selected from H, F, Cl, OR5a, NR7R8, C1-4alkyleneNR7R8 and C1-4alkyl. In an embodiment, R3 is selected from H, F, Cl and C1-4alkyl. In an embodiment, R3 is selected from H, F, Cl, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, and CH2CF3. In an embodiment, R3 is selected from H, F, CF3, CF2H, CH2CF2H and CH3. In an embodiment, R3 is selected from H, F, CF3, CF2H and CH2CF2H. In an embodiment, R3 is selected from H, F, CF3, and CH3. In an embodiment, R3 is selected from H and F. In an embodiment, R3 is F. In an embodiment, R3 is H.
In an embodiment, R3 is OR5a. In an embodiment, R5a is selected from H and C1-4alkyl. In an embodiment, R5a is selected from H, CH3, CH2CH3, CF3, CFH2, CF2H, CH2CF2H, and CH2CF2H. In an embodiment, R5a is selected from H, CH3, CH2CH3, CF3, CFH2, CF2H, CH2CF2H, and CH2CF2H. In an embodiment, R5a is selected from H, CH3, CF3 and CF2H. In an embodiment, R5a is CF2H. Accordingly, in an embodiment, R3 is selected from OH, OCH3, OCH2CH3, OCF3, OCFH2, OCHF2, OCH2CF2H, and OCH2CF2H.
In an embodiment, R3 is selected from NR7R8 and C1-4alkyleneNR7R8. In an embodiment, R3 is selected from NR7R8 and C1-2alkyleneNR7R8.
In an embodiment, R7 and R8 are each independently selected from H and C1-4alkyl. In an embodiment, R7 and R8 are each independently selected from H, CH3 and CF3 In an embodiment, one of R7 and R8 is H and the other is CH3. In an embodiment, R7 and R8 are both CH3. In an embodiment, R7 and R8 are both H.
In an embodiment, R7 and R8 are joined to form, together with the nitrogen atom therebetween, a 3 to 7-membered saturated or unsaturated ring optionally containing one additional heteromoiety selected from N, NH, NC1-6alkyl, O, S, S(O), and SO2, and optionally substituted with one or more of halo and C1-6alkyl. In an embodiment, R7 and R8 are joined to form, together with the nitrogen atom therebetween to form a 3- to 7-membered heterocyclic ring selected from azetidinyl, diazetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiozolidinyl, piperidinyl, diazinanyl (e.g. piperazinyl), morpholinyl and azepanyl, and optionally substituted with one to four of halo and C1-6alkyl. In an embodiment, R7 and R8 are joined to form, together with the nitrogen atom therebetween, a 4- to 6-saturated ring, and optionally substituted with one to four of halo and C1-4alkyl. In an embodiment R7 and R8 are joined to form, together with the nitrogen atom therebetween, aziridinyl, azetidinyl, pyrrolidinyl, or piperidinyl, and optionally substituted with one to three of Cl, F and C1-4alkyl.
In an embodiment, one of X2 and X3 is N and the other is CR4. In an embodiment, X2 is N and X3 is CR4. In an embodiment, X2 is CR4 and X3 is N. In an embodiment, both X2 and X3are, independently, CR4. In an embodiment, both X2 and X3are N.
In an embodiment, X1 is N, and X2 and X3are both, independently, CR4. In an embodiment, X1 is CR3, and one of X2 and X3 is N and the other is CR4. In an embodiment, X1 is CR3, X2 is CR4 and X3 is N. In an embodiment, X1 is CR3 and X2 is N and X3 is CR4. In an embodiment, X1 is CR3 and X2 and X3 are both, independently, CR4. In an embodiment, X1 is CR3 and both X2 and X3 are N.
In an embodiment, each R4 is independently selected from H, halo, and C1-4alkyl. In an embodiment, each R4 is independently selected from H, F, Cl, and C1-4alkyl. In an embodiment, each R4is independently selected from H, F, Cl, CH3, CF3, CH2F, and CHF2. In an embodiment, each R4 is independently selected from H, F, Cl, CH3, and CF3. In an embodiment, each R4 is independently selected from H and F.
In an embodiment, one of X2 and X3 is N and the other is CH. In an embodiment, X2 is N and X3is CH. In an embodiment, X2 is selected from CH, CF, CCl, CCH3, and CCF3 and X3 is CH. In an embodiment, X2 is CF or CCl and X3is CH. In an embodiment, X2 is CH and X3 is selected from CH, CF, CCl, CCH3, and CCF3. In an embodiment, one of X2 and X3 is CF and the other is CH. In an embodiment, X2 is CF and X3 is CH. In an embodiment, X2 and X3 are both CF. In an embodiment, X2 and X3 are both CH. In an embodiment, X2 and X3are both N.
In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O, S, S(O), SO2, and NR5 and/or optionally substituted with one or more of R6.
In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O, S, S(O), SO2, and NR5. In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O, SO2, and NR5. In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O and NR5. In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O and SO2. In an embodiment, Q is C1-3alkylene optionally interrupted by O. In an embodiment, Q is C1-2alkylene optionally interrupted by SO2.
In an embodiment, R5 is selected from H and C1-4alkyl. In an embodiment, R5 is selected from CH3, CH2CH3, CH(CH3)2, C(CH3)4 and CH2CH(CH3)2. In an embodiment, R5 is selected from CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, and CH2CF3. In an embodiment, R5 is selected from CF2H, CH3 and CF3
In an embodiment, R5 and R5a are independently selected from H and C. 4alkyl. In an embodiment, R5 and R5a are independently selected from CH3, CH2CH3, CH(CH3)2, C(CH3)4 and CH2CH(CH3)2. In an embodiment, R5 and R5a are independently selected from CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, and CH2CF3. In an embodiment, R5 and R5a are independently selected from CH3 and CF3
In an embodiment, Q is C1-3alkylene and optionally substituted with one to three of R6. In an embodiment, Q is CH2 or CH2CH2 and optionally substituted with one or two of R6. In an embodiment, Q is CH2. In an embodiment, Q is CH2CH2.
In an embodiment, each R6 is independently selected from ═O, F, Cl, C1-4alkyl, C3-6cycloalkyl, C3-6heterocycloalkyl, C1-6alkyleneC3-6cycloalkyl, C1-6alkyleneC3-6heterocycloalkyl, OH, OC1-6alkyl, NR7aR8a, and C1-6alkyleneNR7aR8a. In an embodiment, each R6 is independently selected from ═O, F, Cl, C1-4alkyl, C3-6cycloalkyl, C3-6heterocycloalkyl, C1-4alkyleneC3-6cycloalkyl, C1-4alkyleneC3-6heterocycloalkyl, OH, OC. 4alkyl, NR7aR8a, and C1-4alkyleneNR7aR8a. In an embodiment, one of R6 is ═O. In an embodiment, Q is substituted with one R6 and R6 is ═O.
In an embodiment, each R6 is independently selected from F, C, OH, C1-4alkyl OC1-4alkyl and NR7aR8a. In an embodiment, each R6 is independently selected from F, C, OH, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, OCH3, OCH2CH3, OCF3, OCF2H, OCH(CH3)2 and NR7aR8a. In an embodiment, each R6 is independently selected from F, C, OH, CH3, CF2H, CF3, CFH2, OCH3, OCF3, OCF2H and NR7aR8a. In an embodiment, one to three of R6 are independently selected from F, C, CH3, CF2H, CF3, OCH3, OCF3, OCF2H and NR7aR8a. In an embodiment, one to three of R6 are independently selected from F, C, CH3, CF2H, CF3, OCH3, OCF3 and OCF2H. In an embodiment, one to four of R6 are independently selected from F, CH3, and OCH3.
In an embodiment, each R6 is independently selected from F, C and C1-4alkyl. In an embodiment, each R6 is independently selected from F, C, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, and CH2CFH2. In an embodiment, each R6 is independently selected from F, C, CH3, CF2H, CF3 and CH2CF2H. In an embodiment, each R6 is independently selected from F, Cl, CH3, and CF3. In an embodiment, each R6 is independently selected from F, CH3, and CF3. In an embodiment, each R6 is independently selected from F, CH3, and CF3. In an embodiment, each R6 is independently selected from F and CH3. In an embodiment, each R6 is independently F. In an embodiment, each R6 is independently CH3. In an embodiment, one or two of R6 is F. In an embodiment, one or two of R6 is CH3.
In an embodiment, each R6 is independently selected from C3-6cycloalkyl, C3-6heterocycloalkyl, C1-4alkyleneC3-6cycloalkyl and C1-4alkyleneC3-6heterocycloalkyl. In an embodiment, each R6 is independently selected from C3-6cycloalkyl, C3-6heterocycloalkyl, C1-2alkyleneC3-6cycloalkyl and C1-2alkyleneC3-6heterocycloalkyl. In an embodiment, the cycloalkyl in R6 is selected from cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In an embodiment, the cycloalkyl in R6 is selected from cyclopropyl and cyclobutyl.
In an embodiment, the heterocycloalkyl R6 is selected from aziridinyl, oxiranyl, thiiranyl, oxaxiridinyl, dioxiranyl, azetidinyl, oxetanyl, thietanyl, diazetidinyl, dioxetanyl, dithietanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, isoxthiolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, piperidinyl, triazolyl, furazanyl, oxadiazolyl, thiadiazolyl, dioxazolyl, dithiazolyl, tetrazolyl, oxatetrazolyl, tetrahydropyranyl, diazinanyl (e.g., piperazinyl), morpholinyl, thiomorpholinyl, dioxanyl and dithianyl. In an embodiment, the heterocycloalkyl in R6 is selected from azetidinyl, oxetanyl, thietanyl, tetrahydrofuranyl, pyrrolidinyl, imidazolidiny and pyrazolidinyl.
In an embodiment, each R6 is independently selected from OH and OC1-4alkyl. In an embodiment, each R6 is independently selected from OH and OC1-4alkyl. In an embodiment, each R6 is independently selected from OH, OCH3, OCH2CH3, OCF3, OCF2H, and OCH(CH3)2. In an embodiment, one or two of R6 are independently selected from OH, OCH3, OCH2CH3, OCF3, OCF2H, and OCH(CH3)2. In an embodiment, one R6 is selected from OH, OCH3, OCF3, and OCF2H.
In an embodiment, one of R6 is NR7aR8a or C1-4alkyleneNR7aR8a. In an embodiment, one of R6 is NR7aR8a or C1-2alkyleneNR7aR8a. In an embodiment, one of R6 is NR7aR8a. In an embodiment, one of R6 is C1-4alkyleneNR7aR8a. In an embodiment, the R7a and R8a in NR7aR8a or C1-2alkylene NR7aR8a of R6 are both CH3 or are both H. In an embodiment, one R4 is NR7aR8a and R7a and R8a in R6 are both H.
In an embodiment, R7a and R8a in R6 are each independently selected from H and C1-4alkyl. In an embodiment, R7a and R8a in R6 are each independently selected from H, CH3, and CF3 In an embodiment, one of R7a and R8a in R6 is H and the other is CH3. In an embodiment, R7a and R8a in R6 are both CH3. In an embodiment, one R6 is NR7aR8a and R7a and R8ain R6 are both CH3 or both H. In an embodiment, one R6 is NR7aR8a and R7a and R8a in R6 are both H.
In an embodiment, R7a and R8a are joined to form, together with the nitrogen atom therebetween, a 3- to 7-saturated or unsaturated ring optionally containing one additional heteromoiety selected from N, NH, NC1-6alkyl, O, S, S(O), and SO2, and optionally substituted with one or more of halo and C1-6alkyl. In an embodiment, R7a and R8a are taken together with the nitrogen atom therebetween to form a 3- to 7-membered heterocyclic ring selected from azetidinyl, diazetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiozolidinyl, piperidinyl, diazinanyl (e.g. piperazinyl), morpholinyl and azepanyl, optionally substituted with one to four of halo and C1-6alkyl. In an embodiment, R7a and R8aare joined to form, together with the nitrogen atom therebetween, a 4- to 6-heterocyclic ring, optionally substituted with one to four of halo and C1-4alkyl. In an embodiment R7a and R8a are joined to form, together with the nitrogen atom therebetween, aziridinyl, azetidinyl, pyrrolidinyl, or piperidinyl, optionally substituted with one to three of Cl, F and C1-4alkylatom.
In an embodiment, Q is unsubstituted. In an embodiment, Q is substituted with one or two of R6. In an embodiment, Q is substituted with one or two of R6, and R6 is C1-4alkyl. In an embodiment, Q is substituted with one or two of R6, and R6 is CH3. In an embodiment, Q is C1-3alkylene and is unsubstituted. In an embodiment, Q is C1-3alkylene and is substituted with one or two of R6. In an embodiment, Q is C1-3alkylene and is substituted with one or two of R6, and one of R6 is C1-4alkyl. In an embodiment, Q is C1-3alkylene and is substituted with one or two of R6, and one of R6 is CH3ln an embodiment, Q is C1-3alkylene and is substituted with one to four of R6, and each R6 is independently selected from F, Cl, CH3, CF2H, CF3, OCH3, OCF3 and OCF2H. In an embodiment, Q is C1-3alkylene and is substituted with one to four of R6, and each R6 is independently selected from F, CH3 and OCH3.
In an embodiment, Q is C1-3alkylene and disubstituted on one carbon atom with R6c and R6b. In an embodiment, Q is C2alkylene or C2alkylene, and disubstituted on one carbon atom with R6a and R6b. In an embodiment, Q is CR6aR6b.
In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 6-membered saturated or unsaturated ring optionally containing one heteromoiety selected from N, NH, NC1-6alkyl, O, S, S(O), and SO2 and optionally substituted with one or more, or one to four, of halo and C1-4alkyl.
In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 6-membered cycloalkyl ring and optionally substituted with one or more of halo and C1-4alkyl. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 6-membered cycloalkyl selected from cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl and optionally substituted with one or more of halo and C1-4alkyl. In an embodiment, R6a and R6b are joined to form, together with the atom therebetween, a 3- to 5-membered cycloalkyl ring, and optionally substituted with one to four of halo and C1-4alkyl. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a cyclopropyl, a cyclobutyl, or a cyclopentyl ring, and optionally substituted with one to three of halo and C1-4alkyl. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a cyclopropyl or a cyclobutyl ring, and optionally substituted with one or two of halo and C1-4alkyl. In an embodiment, R6a and R6bare joined to form, together with the carbon atom therebetween, a cyclopropyl or a cyclobutyl ring. In an embodiment, R6a and R6b are joined to form, together with the carbo atom therebetween, a cyclopropyl ring and optionally substituted with one or two of Cl, F and C1-4alkyl. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a cyclopropyl or a cyclobutyl ring. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, an unsubstituted cyclopropyl ring.
In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 6-membered heterocycloalkyl ring, and optionally substituted with one or more of halo and C1-4alkyl. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 6-membered heterocycloalkyl ring selected from aziridinyl, oxiranyl, thiiranyl, oxaxiridinyl, dioxiranyl, azetidinyl, oxetanyl, thietanyl, diazetidinyl, dioxetanyl, dithietanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, isoxthiolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, piperidinyl, triazolyl, furazanyl, oxadiazolyl, thiadiazolyl, dioxazolyl, dithiazolyl, tetrazolyl, oxatetrazolyl, tetrahydropyranyl, diazinanyl (e.g., piperazinyl), morpholinyl, thiomorpholinyl, dioxanyl, and dithianyl. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 5-membered heterocycloalkyl ring. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 5-membered heterocycloalkyl ring selected from oxiranyl, oxetanyl, azetidinyl, thietanyl, pyrrolidinyl, tetrahydrofuranyl, and tetrahydrothiophenyl ring. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, oxetanyl or azetidinyl. In an embodiment, R6a and R6b are joined to form, together with the carbon atom therebetween, oxetanyl. In an embodiment, R6a and R6b are joined to form, together with the atom therebetween to form herein “·” indicates a point of attachment to Q.
In an embodiment, Q is C2-4alkenylene optionally substituted with one or two of R6c. In an embodiment, Q is C═C optionally substituted with one or two of R6c.
In an embodiment, Q is selected from C═N and N═C and is optionally substituted with R6c. In an embodiment, Q is C═N or N═C.
In an embodiment, each R6cis independently selected from F, Cl, C1-4alkyl, C3-6cycloalkyl, C3-6heterocycloalkyl, C1-6alkyleneC3-6cycloalkyl, C1-6alkyleneC3-6heterocycloalkyl, OH, OC1-6alkyl, NR7aR8a, and C1-6alkyleneNR7aR8a. In an embodiment, each R6c is independently selected from F, Cl, C1-4alkyl, C3-6cycloalkyl, C3-6heterocycloalkyl, C1-4alkyleneC3-6cycloalkyl, C1-4alkyleneC3-6heterocycloalkyl, OH, OC1-4alkyl, NR7aR8a, and C. 4alkyleneNR7aR8a.
In an embodiment, each R6c is independently selected from F, Cl, OH, C. 4alkyl, OC1-4alkyl, C1-2alkyleneNR7aR8a and NR7aR8a. In an embodiment, each R6c is independently selected from F, Cl, OH, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, OCH3, OCH2CH3, OCF3, OCF2H, OCH(CH3)2, C1-2alkyleneNR7aR8a and NR7aR8a. In an embodiment, each R6c is independently selected from F, Cl, OH, CH3, CF2H, CF3, CFH2, OCH3, OCF3, OCF2H and NR7aR8a. In an embodiment, each R6c are independently selected from F, Cl, CH3, CF2H, CF3, OCH3, OCF3, OCF2H, C1-2alkyleneNR7aR8a and NR7aR8a. In an embodiment, each R6c are independently selected from F, Cl, CH3, CF2H, CF3, OCH3, OCF3, OCF2H and NR7aR8a.
In an embodiment, each R6cis independently selected from F and C1-4alkyl. In an embodiment, each R6c is independently selected from F, C, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, and CH2CH2F. In an embodiment, each R6c is independently selected from F, C, CH3, CF2H, CF3 and CH2CF2H. In an embodiment, each R6c is independently selected from F, C, CH3, and CF3. In an embodiment, each R6c is independently selected from F, CH3, and CF3. In an embodiment, each R6c is independently selected from F and CH3. In an embodiment, each R6c is independently F. In an embodiment, each R6c is independently CH3. In an embodiment, each R6c is independently F. In an embodiment, each R6c is independently CH3. In an embodiment, one or two R6c is F. In an embodiment, one or two R6c is CH3. In an embodiment, one or two R6c is F. In an embodiment, each R6c is CH3.
In an embodiment, each R6cis independently selected from C3-6cycloalkyl, C3-6heterocycloalkyl, C1-4alkyleneC3-6cycloalkyl and C1-4alkyleneC3-6heterocycloalkyl. In an embodiment, each R6c is independently selected from C3-6cycloalkyl, C3-6heterocycloalkyl, C1-2alkyleneC3-6cycloalkyl and C1-2alkyleneC3-6heterocycloalkyl. In an embodiment, the cycloalkyl in R6c is selected from cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In an embodiment, the cycloalkyl in R6c is selected from cyclopropyl and cyclobutyl.
In an embodiment, the heterocycloalkyl R6c is selected from aziridinyl, oxiranyl, thiiranyl, oxaxiridinyl, dioxiranyl, azetidinyl, oxetanyl, thietanyl, diazetidinyl, dioxetanyl, dithietanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, isoxthiolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, piperidinyl, triazolyl, furazanyl, oxadiazolyl, thiadiazolyl, dioxazolyl, dithiazolyl, tetrazolyl, oxatetrazolyl, tetrahydropyranyl, diazinanyl (e.g., piperazinyl), morpholinyl, thiomorpholinyl, dioxanyl, and dithianyl. In an embodiment, the heterocycloalkyl in R6c is selected from azetidinyl, oxetanyl, thietanyl, tetrahydrofuranyl, pyrrolidinyl, imidazolidiny, and pyrazolidinyl.
In an embodiment, each R6c is independently selected from OH and OC. 4alkyl. In an embodiment, each R6c is independently selected from OH and OC1-4alkyl. In an embodiment, each R6 is independently selected from OH, OCH3, OCF3, OCF2H, OCH2CH3 and OCH(CH3)2. In an embodiment, one R6c is selected from OH, OCH3, OCF3, and OCF2H.
In an embodiment, one of R6c is NR7aR8a or C1-4alkyleneNR7aR8a. In an embodiment, one of R6c is NR7aR8a or C1-2alkyleneNR7aR8a. In an embodiment, one of R6c is NR7aR8a. In an embodiment, R6c is NR7aR8a and R7a and R8a in R6c are both CH3 or both H. In an embodiment, one R6c is NR7aR8a and R7a and R8a in R6c are both H.
In an embodiment, R7a and R8a in R6care each independently selected from H and C1-4alkyl. In an embodiment, R7a and R8a in R6c are each independently selected from H, CH3, and CF3 In an embodiment, one of R7a and R8a in R6cis H and the other is CH3. In an embodiment, R7a and R8a in R6c are both CH3.
In an embodiment, R7a and R8a in R6c are joined to form, together with the nitrogen atom therebetween, a 3- to 7-membered saturated or unsaturated ring optionally containing one additional heteromoiety selected from N, NH, NC1-6alkyl, O, S, S(O), and SO2, and optionally substituted with one or more of halo and C1-6alkyl. In an embodiment, R7a and R8a in R6c are taken together with the nitrogen atom therebetween to form a 3- to 7-membered heterocyclic ring selected from azetidinyl, diazetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiozolidinyl, piperidinyl, diazinanyl (e.g. piperazinyl), morpholinyl and azepanyl, optionally substituted with one to four of halo and C1-6alkyl. In an embodiment, R7a and R8a in R6c are joined to form, together with the nitrogen atom therebetween, a 4- to 6-membered heterocyclic ring, optionally substituted with one to four of halo and C1-4alkyl. In an embodiment R7a and R8a in R6c are joined to form, together with the nitrogen atom therebetween, aziridinyl, azetidinyl, pyrrolidinyl, or piperidinyl, optionally substituted with one to three of F, Cl and C1-4alkyl.
In an embodiment, Q is C1-3alkylene optionally interrupted by NR5. In an embodiment, R5is selected from H and C1-4alkyl. In an embodiment, R5 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3.
In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O, S, S(O), SO2, and NR5. In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O, SO2, and NR5. In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O and NR5. In an embodiment, Q is C1-3alkylene optionally interrupted by a heteromoiety selected from O and SO2. In an embodiment, Q is C1-3alkylene optionally interrupted by O. In an embodiment, Q is C2alkylene optionally interrupted by O. In an embodiment, Q is C1-2alkylene optionally interrupted by SO2.
In an embodiment, R5is selected from H and C1-4alkyl. In an embodiment, R5 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, and CH2CF3.
In an embodiment, Q is C1alkylene or C2alkylene, and is disubstituted on one carbon atom with R6a and R6b and R6a and R6b are joined to form, together with the carbon atom therebetween, a 3- to 5-membered cycloalkyl ring, and optionally substituted with one or more of halo and C1-4alkyl. In an embodiment, Q is CR6aR6b. In R6a and R6b are joined to form, together with the carbon atom therebetween, a cyclopropyl or a cyclobutyl ring, and optionally substituted with one or more of halo and C1-4alkyl.
In an embodiment, Q is C2-4alkenylene optionally substituted with one or two of R6c , and each R6c is independently selected from F, Cl, C1-4alkyl and OC1-4alkyl. In an embodiment, Q is C═C optionally substituted with one or two of R6c , and each R6c is independently selected from F, Cl, C1-4alkyl and OC1-4alkyl. In an embodiment, Q is C═C optionally substituted with one or two of R6c , and each R6c is independently selected from F, Cl, CH3 and OCH3OCF3 and OCF2H. In an embodiment, Q is C═C optionally substituted with one or two of R6c , and each R6c is independently selected from F and C1-4alkyl. In an embodiment, R6c is selected from F and CH3. In an embodiment, Q is C═C substituted with F. In an embodiment, Q is C═C substituted with C. In an embodiment, Q is C═C substituted with OCH3. In an embodiment, Q is C═C substituted with CH3. In an embodiment, Q is C═C substituted with F and CH3.
In an embodiment, Q is selected from C═N and N═C and is optionally substituted with one or two of R6c , and each R6c is independently selected from F, Cl, C. 4alkyl, OC1-4alkyl, NR7aR8a, cyclopropyl and cyclobutyl. In an embodiment, Q is selected from C═N and N═C and is optionally substituted with one or two of R6c , and each R6c is independently selected from F, Cl, CH3, OCH3, OCF3 OCF2H, NR7aR8a, cyclopropyl and cyclobutyl. In an embodiment, Q is selected from C═N and N═C and is optionally substituted with one or two R6c , and each R6c is independently selected from F, Cl, CH3, OCH3, OCF3 and OCF2H. In an embodiment, Q is selected from C═N and N═C and is optionally substituted with one or two R6c , and each R6c is independently selected from F and CH3. In an embodiment, Q is selected from C═N or N═C and is optionally substituted with one or two R6c , and each R6c is independently selected from cyclopropyl and cyclobutyl. In an embodiment, Q is selected from C═N or N═C and is optionally substituted with one or two R6c , and each R6c is independently selected from F and C1-4alkyl. In an embodiment, Q is selected from C═N and N═C is optionally substituted with R6c , and R6c is selected from F and CH3. In an embodiment, Q is selected from C═N or N═C and is optionally substituted with R6c , and R6c is selected from cyclopropyl and cyclobutyl. In an embodiment, Q is selected from C═N or N═C and is optionally substituted with R6c and R6c is selected from F and C1-4alkyl. In an embodiment, Q is selected from C═N or N═C and is optionally substituted with one or two R6c , and one R6c is NR7aR8a. In an embodiment, Q is selected from C═N or N═C and is optionally substituted with one or two R6c , and one R6c is C1-2alkyleneNR7aR8a.
In an embodiment, R1 and R2 are independently selected from H, F and Cl. In an embodiment, one of R1 and R2 is F or Cl and the other is H. In an embodiment, one of R1 and R2 is F and the other is H. In an embodiment, R1 is F and R2 is H. In an embodiment, R1 is H and R2 is F.
In an embodiment, Cy1 is C6-16aryl or C5-17heteroaryl, and Cy1 is unsubstituted. In an embodiment, Cy1 is C6-16aryl or C5-17heteroaryl, Cy1 is substituted with one to three of R9. In an embodiment, Cy1 is C6-16aryl or C5-17heteroaryl, Cy1 is substituted with Z-Cy2. In an embodiment, Cy1 is C6-16aryl or C5-17heteroaryl, Cy1 is substituted with Z-Cy2 and one to three of R9.
In an embodiment, Cy1 is C6-10aryl. In an embodiment, Cy1 is phenyl, indanyl, or naphthyl. In an embodiment, Cy1 is phenyl.
In an embodiment, Cy1 is a bicyclic C9-11aryl wherein the aryl group is fused to a heterocycloalkyl group, and Cy1 is unsubstituted or is substituted with one or more of R9 or is substituted with Z-Cy2, or is substituted with Z-Cy2 and one or more of R9 . In an embodiment, Cy1 is benzo-fused to a C9-11heterocycloalkyl. In an embodiment, Cy1 is benzo-fused to a C4-8heterocycloalkyl. In an embodiment, Cy1 is selected from indolinyl, dihydrobenzofuranyl, dihydrobenzothiophenyl, benzofuranonyl, 1,1-dioxido-dihydrobenzothiophenyl, benzodioxolyl, benzodioxanyl, tetrahydroisoquinolinyl, tetrahydroquinolinyl, 1-oxo-tetrahydroisoquinolinyl, 1-oxo-tetrahydroquinolinyl, dihydrobenzodioxepinyl, benzoxazinyl, isochromanyl, chromanyl, chromanonyl, isochromenyl, chromenyl, and chromenonyl. In an embodiment, Cy1 is selected from
wherein represents the point of attachment of Cy1 to the remainder of molecule and the Cy1 is unsubstituted or is substituted with one or more of R9, and wherein R9a is selected from H, R9, and Z-Cy2.
In an embodiment, Cy1 is a tricyclic C11-17aryl, wherein the aryl group is fused and/or spirofused to one or two heterocycloalkyl groups and Cy1 is unsubstituted or is substituted with one or more of R9 or is substituted with Z-Cy2, or is substituted with Z-Cy2 and one or more of R9. In an embodiment, Cy1 is a benzofused spirofused tricyclic C11-17aryl. In an embodiment, Cy1 is selected from spirobenzodioxinepiperidinyl, oxospirochromanepiperidinyl, and spirochromanepiperidinyl. In an embodiment, Cy1 is selected from
wherein represents the point of attachment of Cy1 to the remainder of molecule and the Cy1 is unsubstituted or is substituted with one or more of R9, and wherein R9ais selected from H, R9, and Z-Cy2.
In an embodiment, Cy1 is C5-6heteroaryl, and Cy1 is unsubstituted or is substituted with one or more of R9 or is substituted with Z-Cy2, or is substituted with Z-Cy2 and one or more of R9. In an embodiment, Cy1 is selected from pyrrolyl, imidazolyl, oxazolyl, pyrazolyl, thiazolyl, pyridinyl, pyrazinyl, pyridazinyl, and pyrimidinyl. In an embodiment, Cy1 is selected from pyrrolyl, pyrazolyl, thiazolyl, pyridinyl, pyrazinyl, and pyrimidinyl. In an embodiment, Cy1 is selected from pyrazolyl, pyridinyl, pyrazinyl, and pyrimidinyl. In an embodiment, Cy1 is pyridinyl. In an embodiment, Cy1 is pyrazolyl.
In an embodiment, Cy1 is bicyclic C9-11heteroaryl, and Cy1 is unsubstituted or is substituted with one or more of R9or is substituted with Z-Cy2, or is substituted with Z-Cy2 and one or more of R9 In an embodiment, Cy1 is selected from benzofuranyl, benzothiophenyl, benzodioxolyl, quinolinyl, and isoquinolyl.
In an embodiment, Cy1 is a pyrazolodiazepinonyl. In an embodiment, Cy1 is
wherein represents the point of attachment of Cy1 to the remainder of molecule and the Cy1 is unsubstituted or is substituted with one or more of R9, and wherein R9ais selected from H, R9, and Z-Cy2.
In an embodiment, Cy1 is unsubstituted or substituted with one to four of R9. In an embodiment, Cy1 is unsubstituted or substituted with one to three of R9. In an embodiment, Cy1 is unsubstituted or substituted with one to two of R9. In an embodiment, Cy1 is unsubstituted or substituted with one R9. In an embodiment, Cy1 is unsubstituted or substituted with Z-Cy2 and one to four of R9. In an embodiment, Cy1 is unsubstituted or substituted with Z-Cy2 and one to three of R9. In an embodiment, Cy1 is unsubstituted or substituted with Z-Cy2 and one to two of R9. In an embodiment, Cy1 is unsubstituted or substituted with one of Z-Cy2 and R9.
In an embodiment, each R9is independently selected from halo, ═O, CN, NO2, C1-6alkyl, C2-6alkenyl, C2-6alkynyl, OR10, C(O)R10, CO2R10, P(O)R10R11, P(O)(OR10)(OR11), SR10, S(O)R10, SO2R10, S(O)(═NR11)R10, SO2NR10R11, SiR12R12aR12a, C1-6alkyleneOR10, OC1-6alkyleneOR10, C1-6alkyleneNR10aR11a OC1-6alkyleneNR10R11, NR11C1-6alkyleneNR10R11, NR11C1-6alkyleneOR10, NR10aR11a, NR11COR10, NR11CO2R10, NR11SO2R10, C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to three of R13
In an embodiment, each R9 is independently selected from F, Cl, CN, NO2, C1-4alkyl, OR10, C(O)R10, CO2R10, P(O)R10R11, P(O)(OR10)(OR11), SR10, S(O)R10, SO2R10, S(O)(═NR11)R10, SO2NR10R11, SiR12R12aR12b, C1-4alkyleneOR10, OC1-4alkyleneOR10, C1-4alkyleneNR10aR11a, OC1-4alkyleneNR10R11, NR—C1-4alkyleneNR10R11, NR—C1-4alkyleneOR10, NR10aR11a NR11COR10, NR11CO2R10, NR11SO2R10, C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to three of R13.
In an embodiment, each R9 is independently selected from F, Cl, CN, NO2, CH3, CH2CH3, CH(CH3)2, OR10, C(O)R10, Co2R10, P(O)R10R11, P(O)(OR10)(OR11), SR10, S(O)R10, SO2R10, S(O)(═NR11)R10, SO2NR10R11, SiR12R12aR12b, C14alkyleneOR10, OC1-4alkyleneOR10, C1-4alkyleneNR10aR11a OC1-4alkyleneNR10R11, NR11C1-4alkyleneNR10R11, NR11C1-4alkyleneOR10, NR10aR11a, NR11COR10, NR11CO2R10, NR11SO2R10, C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to four of R13.
In an embodiment, each R9 is independently selected from F, CH3, CH2CH3, CH(CH3)2, P(O)R10R11, SO2NR10R11, SiR12R12aR12b, OC1-4alkyleneOR10, C1-4alkyleneNR10aR11a NR10aR11a NR11COR10, NR11SO2R10, C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to three R13. In an embodiment, each R9 is independently selected from F, CH3, CH2CH3, CH(CH3)2, SO2NR10R11, OC1-4alkyleneOR10, NR1-4alkyleneNR10R11, C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to three of R13
In an embodiment, each R9 is independently selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, SO2NR10R11, NR11C1-4alkyleneNR10R11, C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to four of R13 In an embodiment, one or two R9 are selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, SO2NR10R11, NR11C1-4alkyleneNR10R11, C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to four of R13. In an embodiment, one or two R9 are selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, SO2NR10R11, NR11C1-4alkyleneNR10R11, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter four groups being optionally substituted with one to four of R13. In an embodiment, one R11 is SO2NR10R11. In an embodiment, one or two R9 are selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, NR11C1-4alkyleneNR10R11, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13. In an embodiment, one or two R9 are selected from C1-4alkyleneNR10aR11a, NR10aR11a, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13.
In an embodiment, each R9 is independently selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, SO2NR10R11, NR11C1-4alkyleneNR10R11. In an embodiment, one of R9 is SO2NR10R11. In an embodiment, one of R9 is selected from C1-4alkyleneNR10aR11a, NR10aR11a, and NR11C1-4alkyleneNR10R11. In an embodiment, one or two R9 are selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, SO2NR10R11, NR11C1-4alkyleneNR10R11. In an embodiment, one of R9 is SO2NR10R11. In an embodiment, one or two of R9 is selected from C1-4alkyleneNR10aR11a, NR10aR11a, and NR11C1-4alkyleneNR10R11. In an embodiment, one of R9 is NR11C1-4alkyleneNR10R11. In an embodiment, one of R9 is selected from C1-4alkyleneNR10aR11a and NR10aR11a. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a.
In an embodiment, one of R9 is NR10aR11a R10a is selected from H and C1-4alkyl wherein C1-4alkyl is optionally substituted with one to three of R15 and R11a is selected from H and C1-4alkyl. In an embodiment, one of R9 is NR10aR11a, R10a is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3 wherein CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3 are optionally substituted with one to three of R15 and R11a is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF. In an embodiment, one of R9 is NR10aR11a and R10 and R11a are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, one of R9 is NR10aR11a and R10 and R1a are independently selected from H, CF3, CH3 and CH2CH3. Accordingly, in an embodiment, R9 is selected from NH2, N(CH3)2, NH(CH3), N(CH3)(CH2CH2), NH(CH2CH2) and N(CH2CH2). In an embodiment, one of R9 is selected from NH2, N(CH3)2 and NH(CH3).
In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10 is selected from H, C1-4alkyl, C2-4alkenyl, C2-4alkynyl, C1-4alkyleneC3-10cycloalkyl, C1-6alkyleneC3-10heterocycloalkyl, C1-6alkyleneOR14, and C1-6alkyleneNR14aR14b, and all alkyl, alkenyl, alkynyl, alkylene, heterocycloalkyl and cycloalkyl groups in R10a are optionally substituted with one to three of R15 and R10 is selected from H and C1-4alkyl. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10 is selected from H, C1-4alkyl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneOR14, and C1-4alkyleneNR14aR14b and each alkyl, alkylene, heterocycloalkyl and cycloalkyl groups of R10 are optionally substituted with one to three of R15 and R10 is selected from H and C1-4alkyl.
In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10 is selected from H and C1-4alkyl wherein C1-4alkyl is optionally substituted with one to three of R15 and R11a is selected from H and C1-4alkyl. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a, R10a is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3wherein CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3 are optionally substituted with one to three of R15 and R11a is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10a and R11a are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, one of R9 is C1-3alkyleneNR10aR11a and R10 and R11a are independently selected from H, CF3, CH3 and CH2CH3. Accordingly, in an embodiment, R9 is CH2N(CH2CH3)2, C(CH3)2NH2, CH2N(CH3)2, CH2CH2N(CH3)2 and CH2N(CH3)2 and CH2N(CH3)2. In an embodiment, R9 is CH2N(CH3)2. In an embodiment, one of R9 is C1-3alkyleneNR10aR11a and R10a and R11a are independently selected from H and CH3. In an embodiment, one of R9 is C1-3alkyleneNR10aR11a and R10 and R11a are both H or are both CH3.
In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10 is selected from C1-6alkyleneOR14 and C1-6alkyleneNR14aR14b and all alkylene groups in R10a are optionally substituted with one to three of R15. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10 is selected from C1-4alkyleneOR14 and C1-4alkyleneNR14aR14band all alkylene groups in R10a are optionally substituted with one to three of R15. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a R10a is selected from C1-4alkyleneOR14 and C1-4alkyleneNR14aR14b and R11a is selected from H and C1-4alkyl and all alkylene groups in R10a are optionally substituted with one to three of R15. Accordingly, in an embodiment, one of R9 is C1-4alkyleneN(R11a)(C1-6alkyleneOR14) and R11a is selected from H and C1-4alkyl and all alkylene groups in R10a are optionally substituted with one to three of R15.
In an embodiment, one of R9 is C1-4alkyleneNR10aR11a R10a is selected from C1-4alkyleneOR14 and C1-4alkyleneNR14aR14b and R11a is selected from H and C1-4alkyl. In an embodiment, one of R9 is selected from C1-4alkyleneNR10aR11a, R10a is C1-4alkyleneOR14 and C1-4alkyleneNR14aR14b and R11a is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a, R10a is selected from C1-4alkyleneOR14 and C1-4alkyleneNR14aR14b and R11a is selected from H, CH3 and CH2CH3. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a R10a is selected from C1-4alkyleneOR14 and R11a is selected from H and CH3.
In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10 is selected from C1-6alkyleneOR14 and R14 in R10a is selected from H and C1-4alkyl. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10a is selected from C1-6alkyleneOR14 and R14 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10a is selected from C1-6alkyleneOR14 and R14 is selected from H, CF3 and CH3. In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10a is selected from C1-6alkyleneOR14 and each R14 is selected from H and CH3.
Accordingly, in an embodiment, one of R9 is selected from C1-4alkyleneNH(C1-6 alkyleneOH), C1-4alkyleneNCH3(C1-6alkyleneOH), C1-4alkyleneNH(C1-6alkyleneOCH3), C1-4alkyleneNCH3(C1-6alkyleneOCH3). In an embodiment, one of R9 is selected from C1-4alkyleneNH(C1-4alkyleneOH), C1-4alkyleneNCH3(C1-6alkyleneOH), C1-4alkyleneNH(C1-4 alkyleneO CH3) and C1-4alkyleneNCH3(C1-6alkyleneOCH3). In an embodiment, one of R9 is C1-4alkyleneNR10aR11a and R10a is C1-6alkyleneC3-7heterocycloalkyl and R11a is selected from H and C1-4alkyl. In an embodiment, one of R9 is selected from C1-4alkyleneC3-7heterocycloalkyl and R10a is C1-4alkyleneC3-7heterocycloalkyl and R11a is selected from H and C1-4alkyl. In an embodiment, one of R9 is C1-4alkyleneC3-7heterocycloalkyl and R10 is C1-2alkyleneC3-7heterocycloalkyl and R11a is selected from H and C1-4alkyl. In an embodiment, the C3-7heterocycloalkyl in the C1-4alkyleneC3-7heterocycloalkyl of R10 is selected from azetidinyl, oxetanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, pyrrolidin-2-onyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, 5,6-dihydro-1,2,4-triazinyl, 3,4,5,6-tetrahydro-1,2,4-triazinyl, thianyl, piperidinyl, piperazinyl, tetrahydropyranyl, thiomorpholinyl, morpholinyl, dioxanyl, azepanyl, diazepanyl, oxepanyl, thiepanyl, azabicyclohexanyl, azabicycloheptanyl, oxabicyclohexanyl, and oxabicycloheptanyl. In an embodiment, the C3-7heterocycloalkyl in the C1-4alkyleneC3-7heterocycloalkyl of R10 is selected from selected from azetidinyl, tetrahydrofuranyl, pyrrolidinyl, pyrrolidin-2-onyl, piperidinyl, piperazinyl and morpholinyl.
In an embodiment, each R9 is independently selected from C3-7cycloalkyl and C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, optionally substituted with one to four of R13. In an embodiment, one or two R9 are selected from C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, each of which is optionally substituted with one to four of R13. In an embodiment, one R9 is selected from C1-3alkyleneC3-7cycloalkyl and C3-7cycloalkyl, optionally substituted with one or two of R13. In an embodiment, the C3-7cycloalkyl in the C1-3alkyleneC3-7cycloalkyl and C3-7cycloalkyl of R9 is selected from C3-7cycloalkyl selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, bicyclo[2.2.1]hexanyl and bicyclo[2.2.1]heptanyl, optionally substituted with one or two of R13
In an embodiment, one of R9 is selected from C1-3alkyleneC3-7cycloalkyl and C3-7cycloalkyl, optionally substituted with one to three of R13. In an embodiment, the C3-7cycloalkyl in the C1-3alkyleneC3-7cycloalkyl and C3-7cycloalkyl of R9 is selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, bicyclo[2.2.1]hexanyl and bicyclo[2.2.1]heptanyl, optionally substituted with one or two of R13.
In an embodiment, one of R9 is selected from C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, each of which is optionally substituted with one to four of R13 In an embodiment, the C3-7heterocycloalkyl in the C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl of R9 is selected from azetidinyl, oxetanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, pyrrolidin-2-onyl, azabicyclohexanyl, azabicycloheptanyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, 5,6-dihydro-1,2,4-triazinyl, 3,4,5,6-tetrahydro-1,2,4-triazinyl, thianyl, piperidinyl, piperazinyl, tetrahydropyranyl, thiomorpholinyl, morpholinyl, dioxanyl, azepanyl, diazepanyl, oxepanyl, thiepanyl, azabicyclohexanyl, azabicycloheptanyl, oxabicyclohexanyl, and oxabicycloheptanyl optionally substituted with one to three of R13. In an embodiment, one of R9 is selected from C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl, and the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R9 is selected from azetidinyl, oxetanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, pyrrolidin-2-onyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, 5,6-dihydro-1,2,4-triazinyl, 3,4,5,6-tetrahydro-1,2,4-triazinyl, thianyl, piperidinyl, piperazinyl, tetrahydropyranyl, thiomorpholinyl, morpholinyl, and dioxanyl, optionally substituted with one to three of R13. In an embodiment, the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R9 is selected from tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, pyrrolidin-2-onyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl and isothiazolidinyl, optionally substituted with one to three of R13 In an embodiment, the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6 heterocycloalkyl of R9 is selected from pyrrolidinyl, pyrrolidin-2-onyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, and isothiazolidinyl, optionally substituted with one to three of R13
In an embodiment, each R9 is independently selected from C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl, optionally substituted with one to four of R13. In an embodiment, the C3-7heterocycloalkyl in the C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl of R9 is selected from azetidinyl, oxetanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, 5,6-dihydro-1,2,4-triazinyl, 3,4,5,6-tetrahydro-1,2,4-triazinyl, thianyl, piperidinyl, piperazinyl, tetrahydropyranyl, thiomorpholinyl, morpholinyl, dioxanyl, azepanyl, diazepanyl, oxepanyl, thiepanyl, azabicyclohexanyl, azabicycloheptanyl, oxabicyclohexanyl, and oxabicycloheptanyl optionally substituted with one to three of R13. In an embodiment, one of R9 is selected from C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl, and the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R9 is selected from azetidinyl, oxetanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, 5,6-dihydro-1,2,4-triazinyl, 3,4,5,6-tetrahydro-1,2,4-triazinyl, thianyl, piperidinyl, piperazinyl, tetrahydropyranyl, thiomorpholinyl, morpholinyl, and dioxanyl, optionally substituted with one to three of R13. In an embodiment, the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R9 is selected from tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl and isothiazolidinyl, optionally substituted with one to three of R13. In an embodiment, the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R9 is selected from pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, and isothiazolidinyl, optionally substituted with one to three of R13. In some embodiments, the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R13 is selected from pyrrolidin-2-onyl, azabicyclohexanyl and azabicycloheptanyl. In some embodiments, the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R13 is pyrrolidin-2-onyl.
In an embodiment, the C3-7heterocycloalkyl in the C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl of R9 comprises at least one N atom. In an embodiment, the C3-7heterocycloalkyl in the C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl of R9 is selected from azetidinyl, pyrrolidinyl, pyrrolidin-2-onyl, azabicyclohexanyl, azabicycloheptanyl, piperidinyl, piperazinyl and morpholinyl each of which optionally substituted with one or two of R13. In an embodiment, the C4-6heterocycloalkyl in the C4-6heterocycloalkyl and C1-4alkyleneC4-6heterocycloalkyl of R9 is pyrrolidinyl, optionally substituted with one or two of R13.
In an embodiment, one of R9 is C3-7heterocycloalkyl, optionally substituted with one to four of R13 and the C3-7heterocycloalkyl is selected from azabicyclohexanyl, azabicycloheptanyl, pyrrolidinyl and pyrrolidin-2-onyl optionally substituted with one to four of R13. In an embodiment, one of R9 is C4-6heterocycloalkyl, optionally substituted with one to four of R13 and the C4-6heterocycloalkyl is pyrrolidin-2-onyl. In an embodiment, one of R9 is pyrrolidin-2-onyl selected from
optionally substituted with one to three of R13, wherein R13d is selected from H and R13, and indicates a point of covalent attachment to Cy1. In an embodiment, one of R9 is C4-6heterocycloalkyl, optionally substituted with one to four of R13 and the C4-6heterocycloalkyl is pyrrolidinyl. In an embodiment, one of R9 is pyrrolidine selected from
and optionally substituted with one to three of R13, wherein R13d is selected from H and R13, and indicates a point of covalent attachment to Cy1. In an embodiment, one of R9 is C4-6heterocycloalkyl, and the C4-6heterocycloalkyl is pyrrolidinyl selected from
optionally substituted with one or two of R13, wherein R13d is selected from H and R13, and indicates a point of covalent attachment to Cy1. In an embodiment, R13d is H. In an embodiment, R13d is R13.
In an embodiment, one of R9 is C1-6alkyleneC3-7heterocycloalkyl, optionally substituted with one to four of R13, and the C3-7heterocycloalkyl in the C1-6alkyleneC3-7heterocycloalkyl is selected from azetidinyl, pyrrolidinyl, pyrrolidin-2-onyl, piperidinyl, piperazinyl and morpholinyl. In an embodiment, one of R9 is C1-3alkyleneC4-7heterocycloalkyl, optionally substituted with one to four of R13, and the C4-7heterocycloalkyl in the C1-6alkyleneC3-7heterocycloalkyl is selected from azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl and morpholinyl. Accordingly, in an embodiment, one of R9 is selected from C1-3alkyleneazetidinyl, C1-3alkylenepyrrolidinyl, C1-3alkylenepiperidinyl, C1-3alkylenepiperazinyl and C1-3alkylenemorpholinyl optionally substituted with one to four of R13. In an embodiment, one of R9 is selected from CH2azetidinyl, CH2pyrrolidinyl, CH2piperidinyl, CH2piperazinyl and CH2morpholinyl optionally substituted with one to four of R13. In an embodiment, one of R9 is selected from
optionally substituted with one or two of R13, wherein R13d is selected from H and R13, and indicates a point of covalent attachment to Cy1.
In an embodiment, one of R9 is C1-6alkyleneC3-7heterocycloalkyl, optionally substituted with one to four of R13, and the C3-7heterocycloalkyl in the C1-6alkyleneC3-7heterocycloalkyl is pyrrolidinyl. In an embodiment, one of R9 is C1-4alkylenepyrrolidinyl optionally substituted with one to three of R13. In an embodiment, one of R9 is C1-4alkylenepyrrolidinyl optionally substituted with one or two of R13, wherein a R13 is F. In an embodiment, one of R9 is selected from CH2pyrrolidinyl optionally substituted with one to four of R13. In an embodiment, the one of R9 is
wherein indicates a point of covalent attachment to Cy1.
In an embodiment, each R13 is independently selected from F, Cl, CN, OH, C1-4alkyl, OC1-4alkyl and NR13aR13b. In an embodiment, each R13 is independently selected from F, Cl, OC1-4alkyl, C1-4alkyl and NR13aR13b.
In an embodiment, one R13 is selected from OH, OC1-4alkyl and NR13aR13bIn an embodiment, one of R13 is selected from OH and OC1-4alkyl. In an embodiment, one R13 is OC1-4alkyl. In an embodiment, one of R13 is OCH3.
In an embodiment, one of R9 is C1-6alkyleneC3-7heterocycloalkyl as described above, substituted with one or two R13 and one R13 is selected from OH and OC1-4alkyl. In an embodiment, one of R9 is C1-6alkyleneC3-7heterocycloalkyl as described above, substituted with one R13 and R13 is OCH3.
In an embodiment, each R13 is independently selected from F, Cl, CN, C1-4alkyl and NR13aR13b. In an embodiment, each R13 is independently selected from F, C1-4alkyl and NR13aR13b. In an embodiment, one or two R13 is independently selected from F, Cl, CN, C1-4alkyl and NR13aR13b. In an embodiment, one or two R13 is selected from F, C1-4alkyl and NR13aR13b. In an embodiment, one of R13 is F. In an embodiment, one of R13 is NR13aR13b
In an embodiment, R13a and R13b are independently selected from H and C1-4alkyl. In an embodiment, R13a and R13b are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, R13aand R13b are independently selected from H, CF3 and CH3. In an embodiment, R13a and R13b are independently selected from H and CH3.
In an embodiment, one of R9 is selected from C3-7cycloalkyl, C3-7heterocycloalkyl, C1-4alkyleneC3-7cycloalkyl and C1-4alkyleneC3-7heterocycloalkyl which are unsubstituted. In an embodiment, one of R9 is C3-7heterocycloalkyl as defined above which is unsubstituted. In an embodiment, one of R9 is selected from pyrrolidinyl, pyrrolidin-2-onyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, and isothiazolidinyl, which is unsubstituted. In an embodiment, one of R9is pyrrolidinyl which is unsubstituted. In an embodiment, one of R9is selected from pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, and isothiazolidinyl, which is unsubstituted. In an embodiment, one of R9is pyrrolidinyl which is unsubstituted. In an embodiment, Cy1 is substituted with one R9 and R9 is pyrrolidin-2-only which is unsubstituted.
In an embodiment, one of R9 is selected from
wherein indicates a point of covalent attachment to Cy1. In an embodiment, one of R9 is selected from
wherein indicates a point of covalent attachment to Cy1.
In an embodiment, one to three R9 are independently selected from F, Cl, CN, NO2 and C1-4alkyl. In an embodiment, each R9 is independently selected from F, Cl, CN, NO2, CH3, CH2CH3, and CH(CH3)2. In an embodiment, one to three R9are independently selected from F, Cl, CN, NO2, CH3, CH2CH3, CH2CH2CH3, CH2CH2CH2CH3, CH(CH3)2, CH2CH(CH3)2, C(CH3) and CH(CH3)2. In an embodiment, each R9 is independently selected from F, Cl, CN, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CH2F, CH2CF2H, and CH(CH3)2. In an embodiment, one to three R9 are independently selected from F, Cl, CN, CH3, CH2CH3, CH2CH2CH3, CH(CH3)2, CH2CH2CH2CH3, CF2H, CF3, CFH2, CH2CH2F, CH2CF2H, CH2CH2F2H, CH2CH2CH2F2H and CH(CH3)2. In an embodiment, each R9 is independently selected from F, C, CN, CH3, CH2CH3, CF3, CH2CF2H, CH2CF3 and CH(CH3)2 In an embodiment, one to three R9 are independently selected from F, C, CN, CH3, CH2CH3, CF3, CF2H, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H and CH(CH3)2 In an embodiment, one to three R9 are independently selected from CH3, CH2CH3, CF3, CF2H, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H and CH(CH3)2. In an embodiment, one or two R9 are selected from CF2H, CH2CF2H, CH2CH2F2H, and CH2CH2CH2F2H. In an embodiment, one or two R9 are selected from CH2CH2F2H and CH2CH2CH2F2H. In an embodiment, each R9 is independently selected from F, C, CN, CH3, CH2CH3, CF3, CH2CF2H, CH2CF3, and CH(CH3)2. In an embodiment, each R9 is independently selected from F, CN, CH3, CH2CH3, CF3, CH2CF2H, CH2CF3, and CH(CH3)2. In an embodiment each R9is independently selected from CF3, CH2CF3, and F. In an embodiment, one to three R9 are independently selected from F, C, CN, CH3, CH2CH3, CF3, CH2CF2H, CH2CF3, and CH(CH3)2. In an embodiment, one to three R9 are independently selected from F, CN, CH3, CH2CH3, CF3, CH2CF2H, CH2CF3, and CH(CH3)2. In an embodiment, one R9is CN. In an embodiment, one to three R9 are independently selected from CF3, CH2CF3, and F. In an embodiment, one to three R9 are independently selected from CF2H, CH2CF2H, CH2CF2H, CH2CH2F2H and CH2CH2CH2F2H. In an embodiment, one to three of R9 is F. In an embodiment, one of R9 is F.
In an embodiment, each R9 is independently selected from F, CH3, CH2CH3, CH(CH3)2, OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, C1-4alkyleneOR10, OC1-4alkyleneOR10, C1-4alkyleneNR10aR11a, OC1-4alkyleneOR10, NR11C1-4alkyleneOR10, NR11COR10, and NR11SO2R10, and R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkylalkyleneOR14, and C1-2alkylalkyleneNR14aR14b and all alkyl, alkenyl, alkynyl, alkylene and cycloalkyl groups of R10 are optionally substituted with one to three of R15. In an embodiment, one to three R9 are independently selected from OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, C1-4alkyleneOR10, OC1-4alkyleneOR10, NR11C1-4alkyleneOR10, NR11COR10, and NR11SO2R10
In an embodiment, R10 and R10a are independently selected from H, C1-4alkyl, C2-4alkenyl, C2-4alkynyl, C1-4alkyleneC3-10cycloalkyl, C1-6alkyleneC3-10heterocycloalkyl, C1-6alkylalkyleneOR14, and C1-6alkyleneNR14aR14b, and all alkyl, alkenyl, alkynyl, alkylene, 1heterocycloalkyl and cycloalkyl groups of R10 or R10a are optionally substituted with one to three of R15. In an embodiment, R10 and R10a are independently selected from H, C1-4alkyl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneOR14, and C1-4alkylalkyleneNR14aR14b and alkyl, alkenyl, alkynyl, alkylene, heterocycloalkyl and cycloalkyl groups of R10 or R10a are optionally substituted with one to three of R15. In an embodiment, R10 and R10a are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b and all alkyl, alkenyl, alkynyl, alkylene heterocycloalkyl and cycloalkyl groups of R10 or R10a are optionally substituted with one to three of R15. In an embodiment, each R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3), C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b and CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3), C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14bare optionally substituted with one to three of R15.
In an embodiment, R11 and R11a are independently selected from H and C1-4alkyl. In an embodiment, R11 and R11a are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, R11 and R11a are independently selected from H, CF3 and CH3. In an embodiment, R11 and R11a are independently selected from H and CH3. In an embodiment, each R11 is independently selected from H and C1-4alkyl. In an embodiment, each R11 is independently selected from H, CH3, CH2CH3, CH(CH3)2, CH2CH(CH3)2, C(CH3)3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, each R11 is independently selected from H, CF3 and CH3. In an embodiment, each R11 is independently selected from H and CH3.
In an embodiment, R10 and R11 are joined to form, together with the atom(s) therebetween, a 4- to 6-membered saturated or unsaturated ring, optionally containing one additional heteromoiety selected from N, NR16, O, S, SO, and SO2 and optionally substituted with one to three of R15. In an embodiment, R10 and R11are joined to form, together with the atom(s) therebetween, to form a 4- to 6-membered heterocycloalkyl ring selected from diazetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiozolidinyl, piperidinyl, diazinanyl (e.g. piperazinyl), and morpholinyl, and optionally substituted with one to three of R15. In an embodiment, R10 and R11 are joined to form, together with the atom(s) therebetween, to form a 5- to 6-membered heterocycloalkyl ring selected from pyrrolidinyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiozolidinyl, piperidinyl, diazinanyl (e.g. piperazinyl) and morpholinyl, and optionally substituted with one to three of R15.
In an embodiment, one or two R9 are independently selected from OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, C1-4alkyleneOR10, OC1-4alkyleneOR10, NR1C1-4alkyleneOR10, NR11COR10, and NR11SO2R10 and each R10 is independently selected from H, C1-4alkyl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneOR14, and C1-4alkyleneNR14aR14b and alkylene, heterocycloalkyl and cycloalkyl groups of R10 are optionally substituted with one to three of R15 and R11 is selected from H and C1-4alkyl. In an embodiment, one or two R9 are independently selected from OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, C1-4alkyleneOR10, OC1-4alkyleneOR10, NR1C1-4alkyleneOR10, NR11COR10, and NR11SO2R10 and R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3)3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b and CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3)3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14bgroups of R10 are optionally substituted with one to three of R15 and R11 is selected from H and C1-4alkyl.
In an embodiment, one or two R9 are independently selected from SR10, SO2R10, S(O)(═NR11)R10, NR11COR10, and NR11SO2R10 and R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH(CH3)2, CH2CH(CH3)2, C(CH3), C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b and CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH(CH3)2, CH2CH(CH3)2, C(CH3), C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b groups of R10 are optionally substituted with one to three of R15 and R11 is selected from H and C1-4alkyl.
In an embodiment, one or two R9 are independently selected from SR10 and SO2R10, R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3), C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b and all CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3)3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b groups of R10 are optionally substituted with one to three of R15. In an embodiment, one or two R9 are independently selected from SR10 and SO2R10, R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2 and C(CH3)3wherein CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3)3 are optionally substituted with one to three of R15.
In an embodiment, one or two R9 are independently selected from OR10, C(O)R10, CO2R10, C1-4alkyleneOR10, OC1-4alkyleneOR10, NR1C1-4alkyleneOR10 and R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3)3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b and all CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, C(CH3)3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b groups of R10 are optionally substituted with one to three of R15 and R11 is selected from H and C1-4alkyl.
In an embodiment, one or two R9 are independently selected from OR10, C(O)R10, CO2R10, C1-4alkyleneOR10, OC1-4alkyleneOR10, OC1-4alkyleneOR10, NR11C1-4alkyleneOR10 and R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, and C(CH3)3 and wherein CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, and C(CH3)3 in R10 are optionally substituted with one to three of R15 and R11 is selected from H and C1-4alkyl. In an embodiment, one or two R9 are independently selected from OR10, C(O)R10, CO2R10, C1-4alkyleneOR10 and OC1-4alkyleneOR10, and each R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, and C(CH3)3 wherein the CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, and C(CH3)3 in R10 are optionally substituted with one to three of R15. In an embodiment, one R9 is selected from OR10, C1-4alkyleneOR10, OC1-4alkyleneOR10, and R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, and C(CH3)3 wherein the CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH2CH2F2H, CH2CH2CH2F2H, CH(CH3)2, CH2CH(CH3)2, and C(CH3)3 in R10 are optionally substituted with one to three of R15. In an embodiment, one R9 is selected from OH, OCH3, OCFH2, OCH2CF2H, OCH2CH2F2H, OCH2CH2CH2F2H, C1-4alkyleneOH, C1-4alkyleneOCH3, OC1-4alkyleneOH and OC1-4alkyleneOCH3. In an embodiment, one R9 is selected from OH, OCH3, OCFH2, OCH2CF2H, OCH2CH2F2H, OCH2CH2CH2F2H, CH2OH, CH2CH2OH, CH2CH2OCH3, CH2OCH3, OCH2CH2OH and OCH2CH2OCH3.
In an embodiment, one R9 is selected from OR10, C1-4alkyleneOR10, OC1-4alkyleneOR10, and R10 is selected from C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b and alkylene, heterocycloalkyl and cycloalkyl groups in R10 are optionally substituted with one to three of R15. In an embodiment, one R9 is selected from OR10, C1-4alkyleneOR10 and OC1-4alkyleneOR10, and R10 is selected from C1-2alkyleneC3-6cycloalkyl, C1-2alkyleneC4-7heterocycloalkyl, and C1-2alkyleneNR14aR14b and alkylene, heterocycloalkyl and cycloalkyl groups in R10 are optionally substituted with one to three of R15. In an embodiment, the C4-7heterocycloalkyl in R10 is selected from cyclopropyl and cyclobutyl. Therefore, in an embodiment, one R9 is selected from OR10, C1-4alkyleneOR10 and OC1-4alkyleneOR10, and R10 is selected from C1-2alkylenecyclopropyl, C1-2alkylenecyclobutyl, and C1-2alkyleneC4-7heterocycloalkyl. In an embodiment, one R9 is selected from OR10 and C1-4alkyleneOR10, and R10 is selected from C1-2alkylenecyclopropyl, C1-2alkylenecyclobutyl, and C1-2alkyleneC4-7heterocycloalkyl. Accordingly, in an embodiment, one R9 is selected from OC1-2alkylenecyclopropyl, C1-4alkyleneOC1-2alkylenecyclobutyl, C1-4alkyleneOC1-2alkylenebutyl, C1-2alkyleneC4-7heterocycloalkyl and OC1-2alkyleneC4-7heterocycloalkyl.
In an embodiment, one or two R9 are independently selected from C(O)R10 and CO2R10 and each R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3, CH(CH3)2, CH2CH(CH3)2, and C(CH3)3 each of which is optionally substituted with one to three of R15. In an embodiment, one or two R9 is selected from C(O)CH3, C(O)C(CH3)3, CO2CH3 and CO2C(CH3)3.
In an embodiment, each R15 is independently selected from F, Cl, CN, C1-4alkyl and NR15aR15b. In an embodiment, each R15 is independently selected from F, C1-4alkyl and NR15aR15b. In an embodiment, one of R15 is F. In an embodiment, one of R15 is NR15aR15b.
In an embodiment, R15a and R15b are independently selected from H and C1-4alkyl. In an embodiment, R15a and R15b are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, R15a and R15b are independently selected from H, CF3 and CH3. In an embodiment, R15a and R15b are independently selected from H and CH3.
In an embodiment, R12, R12a, and R12b are independently selected from OR17, C1-4alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl. In an embodiment, R12, R12a, and R12b are independently selected from OR17, C1-4alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl. In an embodiment, one of R12, R12a and R12b is OR17 and the two of R12, R12a and R12b are independently selected from OR17, C1-4alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl. In an embodiment, one of R12, R12a and R12b is OR17 and the other two are independently selected from C1-4alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl. In an embodiment, one of R12, R12a and R12b is OR17 and the other two of R12, R12a and R12b are selected from OR17, C1-4alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl.
In an embodiment, R14, R14a, R14b and R16 are independently selected from H and C1-4alkyl. In an embodiment, R14, R14a, R14b and R16 are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, R14, R14a, R14b and R16 are independently selected from H, CF3 and CH3. In an embodiment, R14, R14a, R14b and R16 are independently selected from H and CH3.
In an embodiment, R17 is selected from H, C1-4alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneOR18, and C1-4alkyleneNR18R19. In an embodiment, R17 is selected from H and C1-4alkyl. In an embodiment, R17 is H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, R17 is selected from H and C1-4alkyl. In an embodiment, R17 is H. In an embodiment R17 is CH3.
In an embodiment, R18 and R19 are independently selected from H and C1-4alkyl.
In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with Z-Cy2 and one or two of R9. In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with Z-Cy2 and one or two of R9, and at least one R9 is F. In an embodiment, Cy1 is phenyl which is substituted with Z-Cy2 and one or two of R9, and at least one R9 is F.
In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one or two of R9, or with Z-Cy2 and one or two of R9. In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one or two of R9, Z-Cy2 and one or two of R9, and at least one R9 is F. In an embodiment, Cy1 is phenyl which is substituted with one or two of R9, or with Z-Cy2 and one or two of R9, and at least one R9 is F.
In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one or two of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is selected from OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, NR11COR10, and NR11SO2R10. In an embodiment, Cy1 is phenyl which is substituted with one or two of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is selected from OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, NR11COR10, and NR11SO2R10. In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one or two of R9, and at least one R9 is selected from OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, NR11COR10, and NR11SO2R10. In an embodiment, Cy1 is phenyl which is substituted with one or two of R9, and at least one R9 is selected from OR10, C(O)R10, CO2R10, SR10, SO2R10, S(O)(═NR11)R10, NR11COR10, and NR11SO2R10. In an embodiment, R10 is selected from H, CH3, CH2CH3, CH(CH3)2, C(CH3)3, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneOR14, and C1-2alkyleneNR14aR14b. In an embodiment, R10 is selected from C1-2alkyleneC3-10cycloalkyl, and C1-2alkyleneC3-10heterocycloalkyl.
In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is selected from SO2NR10R11 and NR11C1-6alkyleneNR10R11. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is selected from SO2NR10R11 and NR11C1-6alkyleneNR10R11. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is SO2NR10R11. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is NR11C1-6alkyleneNR10R11. In an embodiment each R10 and R11 are independently selected from H and C1-4alkyl. In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, and at least one R9 is selected from SO2NR10R11 and NR11C1-6alkyleneNR10R11. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, and at least one R9 is selected from SO2NR10R11 and NR11C1-6alkyleneNR10R11. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, and at least one R9 is SO2NR10R11. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, and at least one R9 is NR11C1-6alkyleneNR10R11. In an embodiment each R10 and R11 are independently selected from H and C1-4alkyl.
In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is SiR12R12aR12b. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and at least one R9 is SiR12R12aR12b. In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, and at least one R9 is SiR12R12aR12b. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, and at least one R9 is SiR12R12aR12b. In an embodiment, one of R12, R12a and R12b is OR17 and the two of R12, R12a and R12b are independently selected from H, OR17, C1-4alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl.
In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9 are selected from C1-4alkyleneNR10aR11a, NR10aR11a, SO2NR10R11, NR11COR10, NR11C1-4alkyleneNR1R11, OR10, C1-4alkyleneOR10, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13 In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9 are selected from C1-4alkylene NR10aR11a, NR10aR11a, NR11C1-4alkyleneNR10aR11a, OR10 C1-4alkyleneOR10, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13. In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9 are selected from C1-4alkyleneNR10aR11a. In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9 are selected from C1-4alkylene NR10aR11a, NR10aR11a, OR10, C1-4alkyleneOR10, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13. In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9are selected from C1-4alkylene NR10aR11a and OR10. In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9 are selected from CH2N(CH2CH3)2, C(CH3)2NH2, CH2N(CH3)2, CH2CH2N(CH3)2 and CH2N(CH3)2 and CH2N(CH3)2. In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9 are selected from C1-4alkyleneC3-7heterocycloalkyl optionally substituted with one to four of R13
In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one of R9 is C1-4alkyleneNR10aR11a, R10a is selected from C1-4alkyleneOR14 and C1-4alkyleneNR14aR14b and R11a is selected from H and C1-4alkyl and all alkylene groups in R10a are optionally substituted with one to three of R15. In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three R9 or is substituted with Z-Cy2 and one to three R9 and one R9 is C1-4alkyleneN(C1-6alkyleneOR14)(R11a). In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9 are selected from C1-4alkyleneNR10aR11a and R10a is selected from C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl and R11a is selected from H and C1-4alkyl. In an embodiment, Cy1 is phenyl or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and one or two R9are selected from C1-4alkyleneNR10aR11a and R10a is selected from C3-7heterocycloalkyl and C1-4alkyleneC3-7heterocycloalkyl and R11a is selected from H and C1-4alkyl wherein the C3-7heterocycloalkyl in the C1-4alkyleneC3-7heterocycloalkyl is selected from azetidinyl, pyrrolidinyl, pyrrolidin-2-onyl, piperidinyl, piperazinyl and morpholinyl.
In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and a R9 is pyrrolidinyl optionally substituted with one or more of R13. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9; or is substituted with Z-Cy2 and one to three R9 and a R9 is pyrrolidinyl, optionally substituted with one or more of R13. In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, and a R9 is pyrrolidinyl optionally substituted with one or more of R13. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9; and a R9 is pyrrolidinyl, optionally substituted with one or more of R13
In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and a R9 is C1-4alkylenepyrrolidinyl optionally substituted with one or two of R13. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and a R9 is C1-4alkylenepyrrolidinyl optionally substituted with one or two of R13. In an embodiment, Cy1 is a bicyclic C9-11heterocycloalkyl or spirofused tricyclic C9-16 heterocycloalkyl which is unsubstituted. In an embodiment, Cy1 is phenyl, pyrrole, or pyridinyl which is substituted with one to three of R9, and a R9 is C1-4alkylenepyrrolidinyl optionally substituted with one or two of R13. In an embodiment, Cy1 is phenyl which is substituted with one to three of R9, and a R9 is C1-4alkylenepyrrolidinyl optionally substituted with one or two of R13. In an embodiment, Cy1 is a bicyclic C9-11heterocycloalkyl or spirofused tricyclic C9-16heterocycloalkyl which is unsubstituted.
In an embodiment, Cy1 is a bicyclic C9-11aryl or tricyclic C11-16aryl group is fused and/or spiro fused, to one or two heterocycloalkyl groups, and Cy1 is unsubstituted. In an embodiment, Cy1 is a bicyclic C9-11aryl or tricyclic C11-16aryl wherein the aryl group is fused and/or spiro fused, to one or two heterocycloalkyl groups, and Cy1 is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and each R9 is selected from F, Cl, CN, NO2, C1-4alkyl, OR10, C(O)R10, CO2R10, SR10, SO2R10, C1-4alkyleneNR10aR11a, NR10aR11a and C1-6alkyleneOR10. In an embodiment, Cy1 is a bicyclic C9-11aryl wherein the aryl group is fused to a heterocycloalkyl group, and Cy1 is substituted with one to three of R9, and each R9 is selected from F, Cl, CN, NO2, C1-4alkyl, OR10, C1-4alkyleneNR10aR11a, NR10aR11a and C1-6alkyleneOR10. In an embodiment, Cy1 is a tricyclic C11-16aryl which is unsubstituted or is substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and each R9 is selected from F, C1-4alkyl, OR10, C(O)R10, CO2R10, C1-4alkyleneNR10aR11a, NR10aR11a, and C1-6alkyleneOR10. In an embodiment, Cy1 is a tricyclic C9-16aryl wherein the aryl group is fused and/or spirofused to two heterocycloalkyl groups, and Cy1 is substituted with one to two of R9, or is substituted with Z-Cy2 and one to three R9 and a R9 is selected from OR10, C(O)R10, and CO2R10. In an embodiment, R10 is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CF3 and C(CH3)3.
In an embodiment, Cy1 is a benzo-fused bicyclic C9-11aryl wherein the aryl group is a phenyl and is fused to a heterocycloalkyl group, or tricyclic C11-16aryl wherein the aryl group is phenyl and is fused and/or spiro fused to one or two heterocycloalkyl groups, all of which are substituted with one to three of R9, or is substituted with Z-Cy2 and one to three R9 and R9is selected from CH3, CF2H, CFH2, CH2CF2H, CH2CH2F2H, CH2CH2CH2F2H, OCH3, OCF2H, OCFH2, OCH2CF2H, OCH2CH2F2H, OCH2CH2CH2F2H, C1-4alkyleneNR10aR11a and NR10aR11a
In an embodiment, Z is absent. It is appreciated by a person skilled in the art that when Z is absent, Cy2 is covalently attached to Cy1 by a direct bond.
In an embodiment, Z is selected from C1-4alkylene, O, C(O), CO2, SO2, S(O)(═NHR11b) and NR11b. In an embodiment, Z is selected from C1-4alkylene, O, C(O), and SO2. In an embodiment, Z is O. In an embodiment, Z is SO2.
In an embodiment, Z is selected from C1-6alkyleneO, C1-6alkyleneC(O), C1-6alkyleneCO2, C1-6alkyleneS, C1-6alkyleneS(O), C1-6alkyleneSO2, C1-6alkyleneS(O)(═NR11b) C1-6alkyleneNR11b, OC1-6alkylene, C(O)C1-6alkylene, CO2C1-6alkylene, SC1-6alkylene, S(O)C1-6alkylene, SO2C1-6alkylene, S(O)(═NR11b)C1-6alkylene and NR11bC1-6alkylene. In an embodiment, Z is selected from C1-4alkyleneO, C1-4alkyleneC(O), C1-6alkyleneCO2, C1-4alkyleneS, C1-4alkyleneS(O), C1-4alkyleneSO2, C1-4alkyleneS(O)(═NR11b), C1-4alkyleneNR11b, OC1-4alkylene, C(O)C1-4alkylene, CO2C1-4alkylene, SC1-4alkylene, S(O)C1-4alkylene, SO2C1-4alkylene, S(O)(═NR11b)C1-4alkylene and NR11bC1-6alkylene. In an embodiment, Z is selected from C1-4alkyleneO, C1-4alkyleneC(O), C1-6alkyleneCO2, C1-4alkyleneS, C1-4alkyleneS(O), C1-4alkyleneSO2, C1-4alkyleneNRl1b, OC1-4alkylene, C(O)C1-4alkylene, CO2C1-4alkylene, SC1-4alkylene, S(O)C1-4alkylene, SO2C1-4alkylene, and NR11bC1-6alkylene. In an embodiment, Z is selected from C1-4alkyleneO, C1-4alkyleneC(O), OC1-4alkylene and C(O)C1-4alkylene. In an embodiment, Z is selected from C1-4alkyleneO, C1-4alkyleneC(O), C1-6alkyleneCO2, C1-4alkyleneS, C1-4alkyleneS(O), C1-4alkyleneSO2, C1-4alkyleneNR11b, OC1-4alkylene, C(O)C1-4alkylene, CO2C1-4alkylene, SC1-4alkylene, S(O)C1-4alkylene, SO2C1-4alkylene, and NR11bC1-6alkylene. In an embodiment, Z is selected from OC1-4alkylene and C(O)C1-4alkylene. In an embodiment, Z is OC1-4alkylene.
In an embodiment, R11b is selected from H and C1-4alkyl. In an embodiment, R11b is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CH2CF2H, CH2CH2CH2CF2H, and CH2CF3. In an embodiment, R11b is selected from H and C1-4alkyl. In an embodiment, R11b is selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, R11b is selected from CH2CH2CF2H and CH2CH2CH2CF2H. In an embodiment, R11b is selected from H, CF3 and CH3. In an embodiment, R11b is selected from H and CH3.
In an embodiment, Cy2 is C3-11cycloalkyl and Cy2 is unsubstituted or substituted with one or more of R20. In an embodiment, Cy2 is selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cycloheptyl. In an embodiment, Cy2 is cyclopropyl.
In an embodiment, Cy2 is a monocyclic C3-7heterocycloalkyl and Cy2 is unsubstituted or substituted with one or more of R20. In an embodiment, Cy2 is selected from azetidinyl, oxetanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, 5,6-dihydro-1,2,4-triazinyl, 3,4,5,6-tetrahydro-1,2,4-triazinyl, dioxidothiomorpholino, tetrahydropyridinyl, dihydropyridinyl, dihydropyranyl, thianyl, piperidinyl, piperazinyl, dihydropyranyl, tetrahydropyranyl, thiomorpholinyl, morpholinyl, dioxanyl, azepanyl, diazepanyl, oxepanyl, and thiepanyl and Cy2 is unsubstituted or substituted with one to four of R20. In an embodiment, Cy2 is selected from azetidinyl, 5,6-dihydro-1,2,4-triazinyl, 5,6-dihydro-4H-1,2,4-oxadiazinyl, dioxidothiomorpholino, thiomorpholinyl, tetrahydrofuranyl, tetrahydropyranyl, diazepanyl, pyrrolidinyl, piperidinyl, piperazinyl, and morpholinyl, and Cy2 is unsubstituted or substituted with one to three of R20. In an embodiment, Cy2 is selected from tetrahydrofuranyl, dihydropyranyl, tetrahydropyranyl, pyrrolidinyl, piperidinyl, piperazinyl and morpholinyl, which is substituted with one to three of R20. In an embodiment, Cy2 is selected from pyrrolidinyl, piperidinyl, piperazinyl, and morpholinyl, and Cy2 is unsubstituted or substituted with one or two of R20. In an embodiment, Cy2 is a monocyclic C3-7heterocycloalkyl and Cy2 is unsubstituted or substituted with one or more of R20. In an embodiment, Cy2 is selected from azetidinyl, oxetanyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, imidazolidinyl, pyrazolidinyl, thiazolidinyl, isothiazolidinyl, dioxolanyl, dithiolanyl, 5,6-dihydro-1,2,4-triazinyl, 3,4,5,6-tetrahydro-1,2,4-triazinyl, dioxidothiomorpholino, tetrahydropyridinyl, dihydropyridinyl, dihydropyranyl, thianyl, piperidinyl, piperazinyl, tetrahydropyranyl, thiomorpholinyl, morpholinyl, dioxanyl, azepanyl, diazepanyl, oxepanyl, and thiepanyl and Cy2 is unsubstituted or substituted with one to four of R20. In an embodiment, Cy2 is selected from azetidinyl, 5,6-dihydro-1,2,4-triazinyl, 5,6-dihydro-4H-1,2,4-oxadiazinyl, dioxidothiomorpholino, thiomorpholinyl, tetrahydrofuranyl, tetrahydropyranyl, diazepanyl, pyrrolidinyl, piperidinyl, piperazinyl, and morpholinyl, and Cy2 is unsubstituted or substituted with one to three of R20. In an embodiment, Cy2 is selected from tetrahydrofuranyl, tetrahydropyranyl, pyrrolidinyl, piperidinyl, piperazinyl and morpholinyl, which is substituted with one to three of R20. In an embodiment, Cy2 is selected from pyrrolidinyl, piperidinyl, piperazinyl, and morpholinyl, and Cy2 is unsubstituted or substituted with one or two of R20. In an embodiment, Cy2 is dihydropyranyl. In an embodiment, Cy2 is selected from piperidinyl, piperazinyl, and morpholinyl, and Cy2 is unsubstituted or substituted with one or two of R20. In an embodiment, Cy2 is piperazinyl and Cy2 is unsubstituted or substituted with one or two of R20. In an embodiment, Cy2 is selected from tetrahydrofuranyl, dihydropyranyl, morpholinyl, and tetrahydropyranyl, which is substituted with one to three of R20. In an embodiment, Cy2 is tetrahydropyranyl, which is substituted with one to three of R20.
In an embodiment, Cy2 is selected from
which is unsubstituted or substituted with one to four of R20, and
wherein
In an embodiment, Cy2 is selected from
which is unsubstituted or substituted with one to four of R20, and
wherein
In an embodiment, Cy2 is selected from
which is unsubstituted or substituted with one to four of R20, and
wherein
In an embodiment, Cy2 is a bicyclic heterocycle and Cy2 is unsubstituted or substituted with one to four of R20.In an embodiment, Cy2 is a bridged bicyclic heterocycle, fused bicyclic heterocycle or a spirofused bicyclic heterocycle and Cy2 is unsubstituted or substituted with one to four of R20. In an embodiment, Cy2 is a bridged bicyclic heterocycle and Cy2 is unsubstituted or substituted with one to four of R20.
In an embodiment, Cy2 is a fused bicyclic heterocycle which is unsubstituted or substituted with one to four of R20. In an embodiment, Cy2 is a C6-C10 saturated bicyclic ring in which one or two of the ring carbon atoms is replaced with N, NH, or NR20, depending on the valency requirements of the N, which is unsubstituted or further substituted with one or four of R20. In an embodiment, Cy2 is azabicyclohexanyl, diazabicycloheptanyl, or diazabicyclooctanyl and Cy2 is unsubstituted or substituted with one to three of R20. In an embodiment, Cy2 is bridged azabicyclohexanyl, bridged diazabicycloheptanyl or bridged diazabicyclooctanyl. unsubstituted or substituted with one to three of R20. In an embodiment, Cy2 is selected from the following structures
which is unsubstituted or substituted with one to four of R20, and
wherein
In an embodiment, Cy2 is selected from the following structures:
which is unsubstituted or substituted with one to four of R20, and
wherein
In an embodiment, Cy2 is
wherein R20d is selected from H and R20, and indicates a point of covalent attachment to Z.
In an embodiment, Cy2 is selected from tetrahydrofuropyrrolyl, hexapyrazinooxazinyl, hexahydropyrrolopyrazinyl, and hexahydropyrrolodiazepiny and Cy2 is unsubstituted or substituted with one to four of R20.
In an embodiment, Cy2 is selected from
which is unsubstituted or substituted with one to four of R20; and
wherein indicates a point of covalent attachment to Z.
In an embodiment, Cy2 is spirofused bicyclic heterocycle and Cy2 is unsubstituted or substituted with one to four of R20.In an embodiment, Cy2 is selected from
which is unsubstituted or substituted with one to four of R20; and
wherein R20d is selected from H and R20, and indicates a point of covalent attachment to Z.
It is appreciated by a person skilled in the art that when Z is absent, then in the structures of Cy2 indicates a point of covalent attachment to Cy1.
In an embodiment, Cy2 is unsubstituted. In an embodiment, Cy2 is substituted with one to three of R20. In an embodiment, Cy2 is substituted with one or two of R20. In an embodiment, Cy2 is substituted with one R20.
In an embodiment, each R20 is independently selected from F, Cl, ═O, CN, OH, C1-4alkyl, C2-4alkenyl, C2-4alkynyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C6-11aryl, C5-14heteroaryl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneC6-11aryl, C1-4alkyleneC5-14heteroaryl, OC1-4alkyl, OC2-4alkenyl, OC2-4alkynyl, C1-4alkyleneOR21, OC1-4alkyleneOR21, C1-4alkyleneNR22R23, OC1-4alkyleneNR22R23, SC1-4alkyl, SC2-4alkenyl, SC2-4alkynyl, C(O)C1-4alkyl, C(O)C2-4alkenyl, C(O)C2-4alkynyl, C(O)C3-10cycloalkyl, C(O)C3-10heterocycloalkyl, C(O)C6-11aryl, C(O)C5-14heteroaryl, C(O)C1-4alkyleneC3-10cycloalkyl, C(O)C1-4alkyleneC3-10heterocycloalkyl, C(O)C1-6alkyleneC6-11aryl, C(O)C1-6alkyleneC5-14heteroaryl, C(O)C1-4alkylenylOR21, C(O)C1-4alkyleneNR22R23, C(O)C1-4alkyleneOC1-4 alkyleneNR22R23, C(O)NR22R23, CO2C1-6alkyl, CO2C2-4alkenyl, CO2C2-4alkynyl, CO2C1-4alkyleneOR21, CO2C1-4alkyleneOC1-4alkyleneNR22R23, NR22R23, NR24C3-10cycloalkyl, NR24C3-10heterocycloalkyl, NR24C6-11aryl, NR24C5-14heteroaryl, NR24C1-4alkyleneC3-10cycloalkyl, NR24C1-4alkyleneC3-10heterocycloalkyl, NR24C1-6alkyleneC6-11aryl, NR24C1-6alkyleneC5-14heteroaryl NR24C1-4alkyleneOR21, NR24SO2C1-4alkyl, SO2C1-6alkyl, SO2C2-4alkenyl, SO2C2-4alkynyl, and SO2NR22R23, and alkyl, alkenyl, alkynyl, alkylene, aryl, heteroaryl, heterocycloalkyl, and cycloalkyl groups of R20 are optionally substituted with one to four of R25. In an embodiment, each R20 is independently selected from F, Cl, ═O, CN, OH, C1-4alkyl, C2-4alkenyl, C2-4alkynyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, OC1-4alkyl, OC2-4alkenyl, OC2-4alkynyl, C1-4alkyleneOR21, OC1-4alkyleneOR21, C1-4alkyleneNR22R23, OC1-4alkyleneNR22R23, SC1-4alkyl, SC2-4alkenyl, SC2-4alkynyl, C(O)C1-4alkyl, C(O)C2-4alkenyl, C(O)C2-4alkynyl, C(O)C3-10cycloalkyl, C(O)C3-10heterocycloalkyl, C(O)C1-4alkyleneC3-10cycloalkyl, C(O)C1-4alkyleneC3-10heterocycloalkyl, C(O)C1-4alkylenylOR21, C(O)C1-4alkyleneNR22R23, C(O)C1-4alkyleneOC1-4alkyleneNR22R23, C(O)NR22R23, CO2C1-6alkyl, CO2C2-4alkenyl, CO2C2-4alkynyl, CO2C1-4alkyleneOR21, CO2C1-4alkyleneOC1-4alkyleneNR22R23, NR22R23, NR24C3-10cycloalkyl, NR24C3-10heterocycloalkyl, NR24C1-4alkyleneC3-10cycloalkyl, NR24C1-4alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneOR21, NR24SO2C1-4alkyl, SO2C1-6alkyl, SO2C2-4alkenyl, SO2C2-4alkynyl, and SO2NR22R23, and alkyl, alkenyl, alkynyl, alkylene, and cycloalkyl groups of R20 are optionally substituted with one to four of R25.
In an embodiment, each R20 is independently selected from F, Cl, ═O, CN, OH, NO2, C1-4alkyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C6-11aryl, C5-14heteroaryl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneC6-11aryl, C1-4alkyleneC5-14heteroaryl, OC1-4alkyl, C1-4alkyleneOR21, OC1-4alkyleneOR21, C1-4alkyleneNR22R23, OC1-4alkyleneNR22R23, SC1-4alkyl, C(O)C1-4alkyl, C(O)C3-10cycloalkyl, C(O) C6-11aryl, C(O)C5-14heteroaryl, C(O)C3-10heterocycloalkyl, C(O)C1-4alkyleneC3-10cycloalkyl, C(O)C1-4alkyleneC3-10heterocycloalkyl, C(O)C1-4alkyleneC6-11aryl, C(O)C1-4alkyleneC5-14 heteroaryl, C(O)C1-4alkyleneOR21, C(O)C1-4alkyleneNR22R23, C(O)C1-4alkyleneOC1-4 alkyleneNR22R23, C(O)NR22R23, CO2C1-6alkyl, CO2C1-4alkyleneOR21, CO2C1-4alkyleneOC1-4alkyleneNR22R23, NR22R23, NR24C3-10cycloalkyl, NR24C3-10heterocycloalkyl, NR24C1-4alkyleneC3-10cycloalkyl, NR24C6-11aryl, NR24C5-10heteroaryl, NR24C1-4alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneC6-11aryl, NR24C1-4alkyleneC5-10heteroaryl, NR24C1-4alkyleneOR21, NR24SO2C1-4alkyl, SO2C1-6alkyl, and SO2NR22R23, and alkyl, alkenyl, alkynyl, alkylene, aryl, heteroaryl, heterocycloalkyl and cycloalkyl groups of R20 are optionally substituted with one to three of R25. In an embodiment, each R20 is independently selected from F, Cl, ═O, CN, OH, NO2, C1-4alkyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, OC1-4alkyl, C1-4alkyleneOR21, OC1-4alkyleneOR21, C1-4alkyleneNR22R23, OC1-4alkyleneNR22R23, SC1-4alkyl, C(O)C1-4alkyl, C(O)C3-10cycloalkyl, C(O)C3-10heterocycloalkyl, C(O)C1-4alkyleneC3-10cycloalkyl, C(O)C1-4alkyleneC3-10heterocycloalkyl, C(O)C1-4alkyleneOR21, C(O)C1-4alkyleneNR22R23, C(O)C1-4alkyleneOC1-4alkyleneNR22R23, C(O)NR22R23, CO2C1-6alkyl, CO2C1-4alkyleneOR21, CO2C1-4alkyleneOC1-4alkyleneNR22R23, NR22R23, NR24C3-10cycloalkyl, NR24C3-10heterocycloalkyl, NR24C1-4alkyleneC3-10cycloalkyl, NR24C1-4alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneOR21, NR24SO2C1-4alkyl, SO2C1-6alkyl, and SO2NR22R23, and alkyl, alkenyl, alkynyl, alkylene, and cycloalkyl groups of R20 are optionally substituted with one to three of R25.
In an embodiment, each R20 is independently selected from F, Cl, ═O, CN, OH, NO2, C1-4alkyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C6-11aryl, C5-14heteroaryl, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, C1-2alkyleneC6-11aryl, C1-2alkyleneC5-14heteroaryl, OC1-4alkyl, C1-4alkyleneOR21, C(O)C1-4alkyl, C(O)C3-10cycloalkyl, C(O)C6-11aryl, C(O)C5-14heteroaryl, C(O)C3-10heterocycloalkyl, C(O)C1-4alkyleneC3-10cycloalkyl, C(O)C1-4alkyleneC3-10heterocycloalkyl, C(O)C1-4alkyleneC6-11aryl, C(O)C1-4alkyleneC5-14heteroaryl, C(O)C1-4alkyleneOR21, C(O)C1-4alkyleneNR22R23, C(O)C1-4alkyleneOC1-4alkyleneNR22R23, CO2C1-6alkyl, CO2C1-4alkyleneOR21, NR22R23, NR24C3-10cycloalkyl, NR24C3-10heterocycloalkyl, NR24C6-11aryl, NR24C5-10heteroaryl, NR24C1-2alkyleneC3-10cycloalkyl, NR24C1-2alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneC6-11aryl, NR24C1-4alkyleneC5-10heteroaryl, NR24C1-4alkyleneOR21, NR24SO2C1-4alkyl, and SO2C1-6alkyl, and alkyl, alkenyl, alkynyl, alkylene, aryl, heteroaryl, heterocycloalkyl and cycloalkyl groups of R20 are optionally substituted with one to three of R25. In an embodiment, each R20 is independently selected from F, Cl, ═O, CN, OH, NO2, C1-4alkyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C1-2alkyleneC3-10cycloalkyl, C1-2alkyleneC3-10heterocycloalkyl, OC1-4alkyl, C1-4alkyleneOR21, C(O)C1-4alkyl, C(O)C3-10cycloalkyl, C(O)C3-10heterocycloalkyl, C(O)C1-4alkyleneC3-10cycloalkyl, C(O)C1-4alkyleneC3-10heterocycloalkyl, C(O)C1-4alkyleneOR21, C(O)C1-4alkyleneNR22R23, C(O)C1-4alkyleneOC1-4alkyleneNR22R23, CO2C1-6alkyl, CO2C1-4alkyleneOR21, NR22R23, NR24C3-10cycloalkyl, NR24C3-10heterocycloalkyl, NR24C1-2alkyleneC3-10cycloalkyl, NR24C1-2alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneOR21, NR24SO2C1-4alkyl, and SO2C1-6alkyl, and alkyl, alkenyl, alkynyl, alkylene, and cycloalkyl groups of R20 are optionally substituted with one to three of R25.
In an embodiment, each R20 is independently selected from F, Cl, ═O, CN, OH, CH3, CH2CH3, CH(CH3)2, C(CH3)3, CF2H, CF3, CFH2, CH2CFH2, CH2CF2H, CH2CF3, CH2CH2CF3, CH2CH2CF2H, CH2CH2CH2CF2H, CH2CH2CH2CF3, C3-6cycloalkyl, C3-6heterocycloalkyl, phenyl, C5-14heteroaryl, C1-4alkyleneC3-6cycloalkyl, C1-4alkyleneC3-6heterocycloalkyl, C1-4alkylenephenyl, C1-4alkyleneC5-14heteroaryl, OC1-4alkyl, C1-4alkyleneOH, C1-4alkyleneOCH3, C(O)C1-4alkyl, C(O)C3-6cycloalkyl, C(O)C3-6heterocycloalkyl, C(O)C6-11aryl, C(O)C5-14heteroaryl, C(O)C1-2alkyleneC3-6cycloalkyl, C(O)C1-2alkyleneC3-10heterocycloalkyl, C(O)C1-4alkyleneC6-11aryl, C(O)C1-4alkyleneC5-14heteroaryl, C(O)C1-4alkyleneOCH3, C(O)C1-4alkyleneOH, C(O)C1-4alkyleneNH2, C(O)C1-4alkyleneN(CH3)2, C(O)C1-4alkyleneOC1-4alkyleneN(CH3)2, CO2C1-6alkyl, CO2C1-4alkyleneOC1-6alkyl, NHC1-4 alkyl, NC1-4alkylC1-4alkyl, NHC1-2alkyleneC3-10cycloalkyl, NCH3Cl1-2alkyleneC3-10cycloalkyl, NCH3Cl1-2alkyleneC3-10heterocycloalkyl, NHC1-2alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneOH, NR24C1-4alkyleneOCH3, NHSO2C1-4alkyl, NCH3SO2C1-4alkyl, and SO2C1-6alkyl, and alkyl, alkylene, phenyl, heteroaryl, heterocycloalkyl and cycloalkyl groups of R20 are optionally substituted with one to three of R25, and each R25 is independently selected from F, C, and C1-4alkyl. In an embodiment, each R20 is independently selected from F, C, ═O, CN, OH, CH3, CH2CH3, CH(CH3)2, C(CH3)3, CF2H, CF3, CFH2, CH2CFH2, CH2CF2H, CH2CF3, CH2CH2CF3, CH2CH2CF2H, CH2CH2CH2CF2H, CH2CH2CH2CF3, C3-6cycloalkyl, C3-6heterocycloalkyl, C1-4alkyleneC3-6cycloalkyl, C1-4alkyleneC3-6heterocycloalkyl, OC1-4alkyl, C1-4alkyleneOH, C1-4alkyleneOCH3, C(O)C1-4alkyl, C(O)C3-6cycloalkyl, C(O)C3-6heterocycloalkyl, C(O)C11aryl, C(O) C5-14heteroaryl, C(O)C1-2alkyleneC3-6cycloalkyl, C(O)C1-2alkyleneC3-10heterocycloalkyl, C(O)C1-4alkyleneC6-11aryl, C(O)C1-2alkyleneC5-14heteroaryl, C(O)C1-2alkyleneOCH3, C(O)C1-4alkyleneOH, C(O)C1-4alkyleneNH2, C(O)C1-4alkyleneN(CH3)2, C(O)C1-4alkyleneOC-4alkyleneN(CH3)2, CO2C1-6alkyl, CO2C1-4alkyleneOC1-6alkyl, NHC1-4alkyl, NC1-4alkylC1-4alkyl, NHC1-2alkyleneC3-10cycloalkyl, NCH3Cl1-2alkyleneC3-10cycloalkyl, NHC1-2alkylenephenyl, NHC1-2alkyleneC5-14heteroaryl, NHC1-2alkylenephenyl, NHC1-2alkyleneC5-14heteroaryl, N(CH3)C1-2alkylenephenyl, N(CH3)C1-2alkyleneC5-14heteroaryl, NCH3Cl1-2alkyleneC3-10heterocycloalkyl, NHC1-2alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneOH, NR24C1-4alkyleneOCH3, NHSO2C1-4alkyl, NCH3SO2C1-4alkyl, and SO2C1-6alkyl, and alkyl, alkylene, heterocycloalkyl and cycloalkyl groups of R20 are optionally substituted with one to three of R25, and each R25 is independently selected from F, C, and C1-4alkyl. In an embodiment, each R20 is independently selected from F, C, ═O, CN, OH, CH3, CH2CH3, CH(CH3)2, C(CH3)3, CF2H, CF3, CFH2, CH2CFH2, CH2CF2H, CH2CF3, CH2CH2CF3, CH2CH2CH2CF3, C3-6cycloalkyl, C3-6heterocycloalkyl, C1-4alkyleneC3-6cycloalkyl, C1-4alkyleneC3-6heterocycloalkyl, OC1-4alkyl, C1-4alkyleneOH, C1-4alkyleneOCH3, C(O)C1-4alkyl, C(O)C3-6cycloalkyl, C(O)C3-6heterocycloalkyl, C(O)C1-2alkyleneC3-6cycloalkyl, C(O)C1-2alkyleneC3-10heterocycloalkyl, C(O)C1-4alkyleneOCH3, C(O)C1-4alkyleneOH, C(O)C1-4alkyleneNH2, C(O)C1-4alkyleneN(CH3)2, C(O)C1-4alkyleneOC1-4alkyleneN(CH3)2, CO2C1-6alkyl, CO2C1-4alkyleneOC1-6alkyl, NHC1-4alkyl, NC1-4alkylC1-4alkyl, NHC1-2alkyleneC3-10cycloalkyl, NCH3C1-2alkyleneC3-10cycloalkyl, NCH3C1-2alkyleneC3-10heterocycloalkyl, NHC1-2alkyleneC3-10heterocycloalkyl, NR24C1-4alkyleneOH, NR24C1-4alkyleneOCH3, NHSO2C1-4alkyl, NCH3SO2C1-4alkyl, and SO2C1-6alkyl, and alkyl, alkylene, and cycloalkyl groups of R20 are optionally substituted with one to three of R25, and each R25 is independently selected from F, Cl, and C1-4alkyl. In an embodiment, each R20 is independently selected from CH2CH2CF2H and CH2CH2CH2CF2H.
In an embodiment, each R20 is independently selected from C3-6cycloalkyl, C3-6heteroycloalkyl, C1-4alkyleneC3-6cycloalkyl, and C1-4alkyleneC3-6heterocycloalkyl. In an embodiment, each R20 is independently selected from C3-5cycloalkyl, C3-6heteroycloalkyl, C1-4alkyleneC3-5cycloalkyl, and C1-4alkyleneC3-5heterocycloalkyl. In an embodiments, all cycloalkyl groups of R22 are optionally substituted with one to three of R25
In an embodiment, one R20 is selected from cyclopropyl, cyclobutyl and cyclopentyl each of which are optionally substituted with one to three of R25. In an embodiment, one R20 is selected from cyclopropyl and cyclobutyl each of which are optionally substituted with one to three of R25. In an embodiment, one R20 is selected from cyclopropyl, cyclobutyl and cyclopentyl. In an embodiment, one R20 is selected from cyclopropyl, cyclobutyl and cyclopentyl.
In an embodiment, one R20 is selected from C1-4alkylenecyclopropyl, C1-4 alkylenecyclobutyl and C1-4alkylenecyclopentyl and all cycloalkyl groups of R20 are optionally substituted with one to three of R25. In an embodiment, one R20 is selected from C1-4alkylenecyclopropyl, C1-4alkylenecyclobutyl and C1-4alkylenecyclopentyl. In an embodiment, one R20 is selected from C1-4alkylenecyclopropyl, C1-4alkylenecyclobutyl and C1-4alkylenecyclopentyl and all cycloalkyl groups of R20 are optionally substituted with one to three of R25. In an embodiment, one R20 is selected from C1-4alkylenecyclopropyl, C1-4alkylenecyclobutyl and C1-4alkylenecyclopentyl. In an embodiment, one R20 is C1-3alkylenecyclopropyl optionally substituted with one to three of R25. In an embodiment, one R20 is C1-3alkylenecyclopropyl. In an embodiment, one R20 is C1-3alkylenecyclopropyl selected from
each of which is optionally substituted with one to three of R25.
In an embodiment, one of R20 is selected from phenyl, C6-11heteroaryl, C1-4alkyleneC6-11aryl and C1-4alkyleneC5-14heteroaryl, and aryl and heteroaryl groups of R20 are optionally substituted with one to three of R25. In an embodiment, one of R20 is selected from phenyl, C5-7heteroaryl, C1-4alkylenephenyl and C1-4alkyleneC5-7heteroaryl, and all aryl and heteroaryl groups of R20 are optionally substituted with one to three of R25. In an embodiment, one of R20 is selected from phenyl and C1-2alkylenephenyl optionally substituted with one to three of R25. In an embodiment, one of R20 is selected from C5-7heteroaryl and C1-6alkyleneC5-7heteroaryl, and all heteroaryl groups of R20 are optionally substituted with one to three of R25. In an embodiment, the heteroaryl in C5-7heteroaryl and C1-6alkyleneC5-7heteroaryl, of R25 are selected from pyrrolyl, furanyl, thiophenyl, imidazolyl, pyrazolyl, oxathiolyl, isoxathiolyl, oxaxolyl, isoxazolyl, thiazoyl, isothiazolyl, triazolyl and tetrazolyl. In an embodiment, the heteroaryl in C5-7heteroaryl and C1-6alkyleneC5-7heteroaryl, of R25 is triazolyl. In an embodiment, the triazole is 1,2,3 trizole or 1, 2, 4-triazole.
In an embodiment, one R20 is selected from CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H, CH2CH2CF2H, CH2CH2CH2CF2H and CH2CF3. In an embodiment, one R20 is selected from CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, one R20 is selected from CH2CH3 and CH(CH3)2. In an embodiment, one R20 is selected from CH2CH3 and CH(CH3)2which are optionally fluoro-substituted. In an embodiment, each R20 is independently selected from CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, each R20 is independently selected from CH2CH3 and CH(CH3)2. In an embodiment, each R20 is independently selected from CH2CH3 and CH(CH3)2 which are optionally fluoro-substituted. In an embodiment, one R20 is selected CF2H, CH2CF2H, CH2CH2CF2H, and CH2CH2CH2CF2H.
In an embodiment, one of R20 is selected from C3-6heteroycloalkyl and C1-4alkyleneC3-6heterocycloalkyl. In an embodiment, the C3-6heteroycloalkyl in the C3-6heteroycloalkyl and C1-4alkyleneC3-6heterocycloalkyl of R20 is selected from oxetanyl, tetrahydrofuranyl and tetrahydropyranyl.
In an embodiment, one R20 is CO2C1-6alkyl. In an embodiment, one R20 is CO2C1-6alkyl. In an embodiment, one R20 is selected from CO2CCH3, CO2CH2CH3, CO2CF2H, CO2CF3, CO2CFH2, CO2CH2CF2H, CO2CH2CF3, CO2CH2CH2F2H, CO2CH2CH2CH2F2H, CO2CH(CH3)2, and CO2CH2CH(CH3)2. an embodiment, one R20 is CO2CCH3.
In an embodiment, one R20 is COC1-6alkyl. In an embodiment, one R20 is COC1-6alkyl. In an embodiment, one R20 is selected from COCCH3, COCH2CH3, COCF2H, COCF3, COCFH2, COCH2CF2H, COCH2CF3, COCH2CH2F2H, COCH2CH2CH2F2H, COCH(CH3)2, and COCH2CH(CH3)2. an embodiment, one R20 is COCH3.
In an embodiment, each R20 is independently selected from OH, F, Cl, CF3, CF2H, CH3, CH2CH3, CH2CF2H, CH2CH2CF2H, CH2CH2CH2F2H
OC1-4alkyl, SO2C1-4alkyl,
In an embodiment, each R20 is independently selected from OH, F, Cl, CF3, CH3, CH2CH3,
OC1-4alkyl, SO2C1-4alkyl,
In an embodiment, R21 is selected from H, C1-4alkyl, C1-4alkyleneOC1-6alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C6-11aryl, C5-14heteroaryl, C2-4alkenyl, C2-4alkynyl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneC6-11aryl and C1-4alkyleneC5-14heteroaryl. In an embodiment, R21 is selected from H and C1-4alkyl. In an embodiment, R21 is selected from C1-4alkyleneOC1-6alkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl. In an embodiment, R21 is selected from H, C1-4alkyl, C1-4alkyleneOC1-6alkyl, C3-11cycloalkyl, C3-10heterocycloalkyl, C2-4alkenyl, C2-4alkynyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl. In an embodiment, R21 is selected from H and C1-4alkyl. In an embodiment, R21 is selected from C1-4alkyleneOC1-6alkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl.
In an embodiment, R22 is selected from H and C1-4alkyl.
In an embodiment, R23 is selected from H, C1-4alkyl, C1-4alkyleneOC1-4alkyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C6-11aryl, C5-14heteroaryl, C1-4alkyleneC3-10cycloalkyl, C1-4alkyleneC3-10heterocycloalkyl, C1-4alkyleneC6-11aryl and C1-4alkyleneC5-14heteroaryl. In an embodiment, R23 is selected from H, C1-4alkyl, C1-4alkyleneOC1-4alkyl, C3-10cycloalkyl, C3-10heterocycloalkyl, C1-4alkyleneC3-10cycloalkyl, and C1-4alkyleneC3-10heterocycloalkyl.
In an embodiment, R22 and R23 are each independently selected from H and C1-4alkyl. In an embodiment, R22 and R23 are joined to form, together with the nitrogen atom therebetween, a 4- to 6-membered saturated or unsaturated ring, optionally containing one additional heteromoiety selected from N, NR26, O, S, S(O), and SO2 and optionally substituted with one to three of halo and C1-6alkyl.
In an embodiment, R24 and R26 are independently selected from H and C. 4alkyl.
In an embodiment, each R25 is independently selected from F, Cl, CN, C. 4alkyl and NR25aR25b. In an embodiment, each R25 is independently selected from F, C1-4alkyl and NR25aR25b. In an embodiment, at least one of R25 is F. In an embodiment, one of R25 is NR25aR25b.
In an embodiment, R25a and R25b are independently selected from H and C. 4alkyl. In an embodiment, R25a and R25b are independently selected from H, CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, R25a and R25b are independently selected from H, CF3 and CH3. In an embodiment, R25a and R25b are independently selected from H and CH3.
In an embodiment, Cy2 is pyrrolidinyl, piperidinyl, piperazinyl or morpholinyl, and each R20 is independently CH3, CH2CH3, CF2H, CF3, CFH2, CH2CF2H and CH2CF3. In an embodiment, Cy2 is pyrrolidinyl, piperidinyl, piperazinyl or morpholinyl, and each R20 is independently CH2CH3 or CH(CH3)2.
In an embodiment, Cy2 is pyrrolidinyl, piperidinyl, piperazinyl or morpholinyl, and one of R20 is C1-3alkylenecyclopropyl.
In an embodiment, the compound of Formula (I) is a compound of Formula (I-A) or (I-B) or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein R1 R2, R6, X1, X2, X3, and Cy1 are as defined in Formula (I) above; and
In an embodiment, the compound of Formula (I) is a compound of Formula (I-C) or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein Q, X1, X2, X3, and Cy1 are as defined in Formula (I) above.
In an embodiment, the compound of Formula (I) is a compound of Formula (I-D), or (I-E), or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein Q, R1, R2, R9, R20, X1, X2, X3, Z, Cy1, and Cy2 are as defined in Formula (I) above;
In an embodiment, the compound of Formula (I) is a compound of Formula (I-F) or (I-G), or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein Q, R9, R20, X1, X2, X3, Z, Cy1, and Cy2 are as defined in Formula (I) above;
In an embodiment, the compound of Formula (I) is a compound of Formula (I-H), or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein R1, R2, R6c, X1, X2, X3, and Cy1 are as defined in Formula (I) above; and
In an embodiment, the compound of Formula (I) is a compound of Formula (I-J) or (I-K) or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein R1, R2, R6c , X1, X2, X3, and Cy1 are as defined in Formula (I) above; and
In an embodiment, the compound of Formula (I) is a compound of Formula (I-L) or (I-M) or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein R6c , R9, X1, X2, X3, Z, Cy1 and Cy2 are as defined in Formula (I) above;
In an embodiment, R6c in the formula of (I-L) or (I-M) is selected from F, Cl, OH, C1-4alkyl, OC1-4alkyl and NR7aR8a. In an embodiment, one or two R6c are selected from F, Cl, CH3, CF2H, CF3, OCH3, OCF3, OCF2H and NR7aR8a
In an embodiment, the compound of Formula (I) is a compound of Formula (I-N) or (I-O) or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein R6c , R9, X1, X2, X3, Z, Cy1 and Cy2 are as defined in Formula (I) above;
In an embodiment, the compound of Formula (I) is a compound of Formula (I-P) or (I-Q), or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein Q, R20, X1, X2, X3, Z, Cy1, and Cy2 are as defined in Formula (I) above;
In an embodiment, one R9 in the of Formula (I-P) or (I-Q) is selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, NR11C1-4alkyleneNR10R11, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13 as defined above for Formula (I). In an embodiment, one or two R9 in the of Formula (I-P) or (I-Q) is are selected from C1-4alkyleneNR10aR11a, NR10aR11a, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13 as described above for Formula (I). In an embodiment, one R9 in the of Formula (I-P) or (I-Q) is C1-2alkyleneNR10aR11a as described above for Formula (I). In an embodiment ff and gg are 1 or 2, suitably 1.
In an embodiment, the compound of Formula (I) is a compound of Formula (I-R) or (I-S) or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein R6c, X1, X2, X3, Z, Cy1 and Cy2 are as defined in Formula (I) above;
In an embodiment, ii and kk are both 1, and R6cin the formula of (I-R) or (I-S) is selected from F and Cl. In an embodiment, ii and kk are both 1, R6c in the formula of (I-R) or (I-S is F.
In an embodiment, jj is 1 or 2, suitably 1. In an embodiment, II is 1 or 2, suitably 1. In an embodiment, one R9 in the of Formula (I-R) or (I-S) s selected from C1-4alkyleneNR10aR11a, NR10aR11a, NR11COR10, NR11C1-4alkyleneNR10R11, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13 as defined above for Formula (I). In an embodiment, one or two R9 in the of Formula (I-R) or (I-S) are selected from C1-4alkyleneNR10aR11a, NR10aR11a, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13 as defined above for Formula (I). In an embodiment, one R9 in the of Formula (I-R) or (I-S) is C1-2alkyleneNR10aR11a as defined above for Formula (I).
In an embodiment, the compound of Formula (I) is a compound of Formula (I-T) or (I-U) or a pharmaceutically acceptable salt, solvate, and/or prodrug thereof:
wherein R6c , X1, X2, X3, Z, Cy1 and Cy2 are as defined in Formula (I) above;
In an embodiment, nn is 1 or 2, suitably 1. In an embodiment, oo is 1 or 2, suitably 1. In an embodiment, one R9 in the of Formula (I-T) or (I-U) is selected from C1-4alkyleneNR10aR11a NR10aR11a, NR11R, NR11C1-4alkyleneNR10R11, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13 as defined above for Formula (I). In an embodiment, one or two R9 in the of Formula (I-T) or (I-U) are selected from C1-4alkyleneNR10aR11a, NR10aR1a, C3-7heterocycloalkyl, and C1-4alkyleneC3-7heterocycloalkyl, the latter two groups being optionally substituted with one to four of R13 as defined above for Formula (I). In an embodiment, one R9 in the of Formula (I-T) or (I-U) is C1-2alkyleneNR10aR11a as defined above for Formula (I).
In an embodiment, the compound of Formula (I) is selected from the compounds listed in Table 1 below:
or a pharmaceutically acceptable salt, solvate and/or prodrug thereof.
In embodiments of the present application, the compounds described herein may have at least one asymmetric center. Where compounds possess more than one asymmetric center, they may exist as diastereomers. It is to be understood that all such isomers and mixtures thereof in any proportion are encompassed within the scope of the present application. It is to be further understood that while the stereochemistry of the compounds may be as shown in any given compound listed herein, such compounds may also contain certain amounts (for example, less than 20%, suitably less than 10%, more suitably less than 5%) of compounds of the present application having an alternate stereochemistry. It is intended that any optical isomers, as separated, pure or partially purified optical isomers or racemic mixtures thereof are included within the scope of the present application.
The compounds of the present application may also exist in different tautomeric forms and it is intended that any tautomeric forms which the compounds form, as well as mixtures thereof, are included within the scope of the present application.
The compounds of the present application may further exist in varying polymorphic forms and it is contemplated that any polymorphs, or mixtures thereof, which form are included within the scope of the present application.
The compounds of the present application also include compounds having alternate isotopes, including radioactive and non-radioactive isotopes, for any of the atoms. For example, in an embodiment, the compounds of the application include compounds wherein one or more available hydrogen atoms have been substituted with deuterium. in an embodiment, the compounds of the application include compounds wherein one or more available carbon atoms have been substituted with 13C.
In an embodiment the pharmaceutically acceptable salt is an acid addition salt or a base addition salt. The selection of a suitable salt may be made by a person skilled in the art (see, for example, S. M. Berge, et al., “Pharmaceutical Salts,” J. Pharm. Sci. 1977, 66, 1-19).
An acid addition salt suitable for, or compatible with, the treatment of subjects is any non-toxic organic or inorganic acid addition salt of any basic compound. Basic compounds that form an acid addition salt include, for example, compounds comprising an amine group. Illustrative inorganic acids which form suitable salts include hydrochloric, hydrobromic, sulfuric, nitric and phosphoric acids, as well as acidic metal salts such as sodium monohydrogen orthophosphate and potassium hydrogen sulfate. Illustrative organic acids which form suitable salts include mono-, di- and tricarboxylic acids. Illustrative of such organic acids are, for example, acetic, trifluoroacetic, propionic, glycolic, lactic, pyruvic, malonic, succinic, glutaric, fumaric, malic, tartaric, citric, ascorbic, maleic, hydroxymaleic, benzoic, hydroxybenzoic, phenylacetic, cinnamic, mandelic, salicylic, 2-phenoxybenzoic, p-toluenesulfonic acid and other sulfonic acids such as methanesulfonic acid, ethanesulfonic acid and 2-hydroxyethanesulfonic acid. In an embodiment, the mono- or di-acid salts are formed, and such salts exist in either a hydrated, solvated or substantially anhydrous form. In general, acid addition salts are more soluble in water and various hydrophilic organic solvents, and generally demonstrate higher melting points in comparison to their free base forms. The selection criteria for the appropriate salt will be known to one skilled in the art. Other non-pharmaceutically acceptable salts such as but not limited to oxalates may be used, for example in the isolation of compounds of the application for laboratory use, or for subsequent conversion to a pharmaceutically acceptable acid addition salt.
A base addition salt suitable for, or compatible with, the treatment of subjects is any non-toxic organic or inorganic base addition salt of any acidic compound. Acidic compounds that form a basic addition salt include, for example, compounds comprising a carboxylic acid group. Illustrative inorganic bases which form suitable salts include lithium, sodium, potassium, calcium, magnesium or barium hydroxide as well as ammonia. Illustrative organic bases which form suitable salts include aliphatic, alicyclic or aromatic organic amines such as isopropylamine, methylamine, trimethylamine, picoline, diethylamine, triethylamine, tripropylamine, ethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperazine, piperidine, N-ethylpiperidine, polyamine resins, and the like. Exemplary organic bases are isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexylamine, choline, and caffeine. The selection of the appropriate salt may be useful, for example, so that an ester functionality, if any, elsewhere in a compound is not hydrolyzed. The selection criteria for the appropriate salt will be known to one skilled in the art.
Solvates of compounds of the application include, for example, those made with solvents that are pharmaceutically acceptable. Examples of such solvents include water (resulting solvate is called a hydrate) and ethanol and the like.
Prodrugs of the compounds of the present application may be, for example, conventional esters formed with available hydroxy, thiol, amino or carboxyl groups. Some common esters which have been utilized as prodrugs are phenyl esters, aliphatic (C1C24) esters, acyloxymethyl esters, carbamates and amino acid esters.
The compounds of the present application are suitably formulated in a conventional manner into compositions using one or more carriers. Accordingly, the present application also includes a composition comprising one or more compounds of the application and a carrier. The compounds of the application are suitably formulated into pharmaceutical compositions for administration to subjects in a biologically compatible form suitable for administration in vivo. Accordingly, the present application further includes a pharmaceutical composition comprising one or more compounds of the application and a pharmaceutically acceptable carrier.
A compound of the application including salts and/or solvates thereof is suitably used on their own but will generally be administered in the form of a composition in which the one or more compounds of the application (the active ingredient) is in association with an acceptable carrier. Depending on the mode of administration, the composition will comprise from about 0.05 wt % to about 99 wt % or about 0.10 wt % to about 70 wt %, of the active ingredient, and from about 1 wt % to about 99.95 wt % or about 30 wt % to about 99.90 wt % of an acceptable carrier, all percentages by weight being based on the total composition.
The compounds of the application may be administered to a subject in a variety of forms depending on the selected route of administration, as will be understood by those skilled in the art. A compound of the application may be administered, for example, by oral, parenteral, buccal, sublingual, nasal, rectal, patch, pump or transdermal administration and the pharmaceutical compositions formulated accordingly. Administration can be by means of a pump for periodic or continuous delivery. Conventional procedures and ingredients for the selection and preparation of suitable compositions are described, for example, in Remington's Pharmaceutical Sciences (2000-20th edition) and in The United States Pharmacopeia: The National Formulary (USP 24 NF19) published in 1999.
Parenteral administration includes intravenous, intra-arterial, intraperitoneal, subcutaneous, intramuscular, transepithelial, nasal, intrapulmonary (for example, by use of an aerosol), intrathecal, rectal and topical (including the use of a patch or other transdermal delivery device) modes of administration. Parenteral administration may be by continuous infusion over a selected period of time.
The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists.
A compound of the application may be orally administered, for example, with an inert diluent or with an assimilable edible carrier, or it may be enclosed in hard or soft shell gelatin capsules, or it may be compressed into tablets, or it may be incorporated directly with the food of the diet. For oral therapeutic administration, the compound may be incorporated with excipient and used in the form of ingestible tablets, buccal tablets, troches, capsules, caplets, pellets, granules, lozenges, chewing gum, powders, syrups, elixirs, wafers, aqueous solutions and suspensions, and the like. In the case of tablets, carriers that are used include lactose, corn starch, sodium citrate and salts of phosphoric acid. Pharmaceutically acceptable excipients include binding agents (e.g., pregelatinized maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium phosphate); lubricants (e.g., magnesium stearate, talc or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulphate). The tablets may be coated by methods well known in the art. In the case of tablets, capsules, caplets, pellets or granules for oral administration, pH sensitive enteric coatings, such as Eudragits™ designed to control the release of active ingredients are optionally used. Oral dosage forms also include modified release, for example immediate release and timed-release, formulations. Examples of modified-release formulations include, for example, sustained-release (SR), extended-release (ER, XR, or XL), time-release or timed-release, controlled-release (CR), or continuous-release (CR or Contin), employed, for example, in the form of a coated tablet, an osmotic delivery device, a coated capsule, a microencapsulated microsphere, an agglomerated particle, e.g., as of molecular sieving type particles, or, a fine hollow permeable fiber bundle, or chopped hollow permeable fibers, agglomerated or held in a fibrous packet. Timed-release compositions can be formulated, e.g. liposomes or those wherein the active compound is protected with differentially degradable coatings, such as by microencapsulation, multiple coatings, etc. Liposome delivery systems include, for example, small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine or phosphatidylcholines. For oral administration in a capsule form, useful carriers or diluents include lactose and dried corn starch.
Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they are suitably presented as a dry product for constitution with water or other suitable vehicle before use. When aqueous suspensions and/or emulsions are administered orally, the compound of the application is suitably suspended or dissolved in an oily phase that is combined with emulsifying and/or suspending agents. If desired, certain sweetening and/or flavoring and/or coloring agents may be added. Such liquid preparations for oral administration may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, methyl cellulose or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters or ethyl alcohol); and preservatives (e.g., methyl or propyl p-hydroxybenzoates or sorbic acid). Useful diluents include lactose and high molecular weight polyethylene glycols.
It is also possible to freeze-dry the compounds of the application and use the lyophilizates obtained, for example, for the preparation of products for injection.
A compound of the application may also be administered parenterally. Solutions of a compound of the application can be prepared in water suitably mixed with a surfactant such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, DMSO and mixtures thereof with or without alcohol, and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms. A person skilled in the art would know how to prepare suitable formulations. For parenteral administration, sterile solutions of the compounds of the application are usually prepared, and the pH of the solutions are suitably adjusted and buffered. For intravenous use, the total concentration of solutes should be controlled to render the preparation isotonic. For ocular administration, ointments or droppable liquids may be delivered by ocular delivery systems known to the art such as applicators or eye droppers. Such compositions can include mucomimetics such as hyaluronic acid, chondroitin sulfate, hydroxypropyl methylcellulose or polyvinyl alcohol, preservatives such as sorbic acid, EDTA or benzyl chromium chloride, and the usual quantities of diluents or carriers. For pulmonary administration, diluents or carriers will be selected to be appropriate to allow the formation of an aerosol.
The compounds of the application may be formulated for parenteral administration by injection, including using conventional catheterization techniques or infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as sterile suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulating agents such as suspending, stabilizing and/or dispersing agents. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. Alternatively, the compounds of the application are suitably in a sterile powder form for reconstitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.
Compositions for nasal administration may conveniently be formulated as aerosols, drops, gels and powders.
For intranasal administration or administration by inhalation, the compounds of the application are conveniently delivered in the form of a solution, dry powder formulation or suspension from a pump spray container that is squeezed or pumped by the patient or as an aerosol spray presentation from a pressurized container or a nebulizer. Aerosol formulations typically comprise a solution or fine suspension of the active substance in a physiologically acceptable aqueous or non-aqueous solvent and are usually presented in single or multidose quantities in sterile form in a sealed container, which can take the form of a cartridge or refill for use with an atomising device. Alternatively, the sealed container may be a unitary dispensing device such as a single dose nasal inhaler or an aerosol dispenser fitted with a metering valve which is intended for disposal after use. Where the dosage form comprises an aerosol dispenser, it will contain a propellant which can be a compressed gas such as compressed air or an organic propellant such as fluorochlorohydrocarbon. Suitable propellants include but are not limited to dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, heptafluoroalkanes, carbon dioxide or another suitable gas. In the case of a pressurized aerosol, the dosage unit is suitably determined by providing a valve to deliver a metered amount. The pressurized container or nebulizer may contain a solution or suspension of the active compound. Capsules and cartridges (made, for example, from gelatin) for use in an inhaler or insufflator may be formulated containing a powder mix of a compound of the application and a suitable powder base such as lactose or starch. The aerosol dosage forms can also take the form of a pump-atomizer.
Compositions suitable for buccal or sublingual administration include tablets, lozenges, and pastilles, wherein the active ingredient is formulated with a carrier such as sugar, acacia, tragacanth, or gelatin and glycerine. Compositions for rectal administration are conveniently in the form of suppositories containing a conventional suppository base such as cocoa butter.
Suppository forms of the compounds of the application are useful for vaginal, urethral and rectal administrations. Such suppositories will generally be constructed of a mixture of substances that is solid at room temperature but melts at body temperature. The substances commonly used to create such vehicles include but are not limited to theobroma oil (also known as cocoa butter), glycerinated gelatin, other glycerides, hydrogenated vegetable oils, mixtures of polyethylene glycols of various molecular weights and fatty acid esters of polyethylene glycol. See, for example: Remington's Pharmaceutical Sciences, 16th Ed., Mack Publishing, Easton, P A, 1980, pp. 1530-1533 for further discussion of suppository dosage forms.
Compounds of the application may also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxy-ethylaspartamide-phenol, or polyethyleneoxide-polylysine substituted with palmitoyl residues. Furthermore, compounds of the application may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyglycolic acid, copolymers of polylactic and polyglycolic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and crosslinked or amphipathic block copolymers of hydrogels.
In an embodiment, compounds of the application may be coupled with viral, non-viral or other vectors. Viral vectors may include retrovirus, lentivirus, adenovirus, herpesvirus, poxvirus, alphavirus, vaccinia virus or adeno-associated viruses. Non-viral vectors may include nanoparticles, cationic lipids, cationic polymers, metallic nanoparticles, nanorods, liposomes, micelles, microbubbles, cell-penetrating peptides, or lipospheres. Nanoparticles may include silica, lipid, carbohydrate, or other pharmaceutically acceptable polymers.
In some embodiments, depending on the mode of administration, the pharmaceutical composition will comprise from about 0.05 wt % to about 99 wt % or about 0.10 wt % to about 70 wt %, of the active ingredient (one or more compounds of the application), and from about 1 wt % to about 99.95 wt % or about 30 wt % to about 99.90 wt % of one or more pharmaceutically acceptable carriers, all percentages by weight being based on the total composition.
In an embodiment, a compound of the present application is administered with another therapeutic agent simultaneously or sequentially in separate unit dosage forms or together in a single unit dosage form. Accordingly, the present application provides a single unit dosage form comprising one or more compounds of the application (e.g. a compound of Formula (I)), an additional therapeutic agent, and a pharmaceutically acceptable carrier.
To be clear, in the above, the term “a compound” also includes embodiments wherein one or more compounds are referenced.
The compounds of the application have been shown to be capable of inhibiting HPK1 activity. In an embodiment, the HPK1 is human HPK1, see for example, Hu, M. C. et. al.; Genes Dev. 10 (1): 2251-2264, 1996.
Accordingly, the present application includes a method for inhibiting HPK1, in a cell, either in a biological sample or in a patient, comprising administering an effective amount of one or more compounds of the application to the cell. The application also includes a use of one or more compounds of the application for inhibiting HPK1 in a cell as well as a use of one or more compounds of the application for the preparation of a medicament for inhibiting HPK1 in a cell. The application further includes one or more compounds of the application for use in inhibiting HPK1.
As the compounds of the application have been shown to be capable of inhibiting HPK1, the compounds of the application are useful for treating diseases, disorders or conditions by inhibiting HPK1. Therefore the compounds of the present application are useful as medicaments. Accordingly, the present application includes a compound of the application for use as a medicament.
The present application also includes a method of treating a disease, disorder or condition that is treatable by inhibiting HPK1 comprising administering a therapeutically effective amount of one or more compounds of the application to a subject in need thereof.
The present application also includes a use of one or more compounds of the application for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1 as well as a use of one or more compounds of the application for the preparation of a medicament for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1. The application further includes one or more compounds of the application for use in treating a disease, disorder or condition that is treatable by inhibiting HPK1.
In an embodiment, the disease, disorder or condition that is treatable by inhibiting HPK1 is a neoplastic disorder. Accordingly, the present application also includes a method of treating a neoplastic disorder comprising administering a therapeutically effective amount of one or more compounds of the application to a subject in need thereof. The present application also includes a use of one or more compounds of the application for treatment of a neoplastic disorder as well as a use of one or more compounds of the application for the preparation of a medicament for treatment of a neoplastic disorder. The application further includes one or more compounds of the application for use in treating a neoplastic disorder. In an embodiment, the treatment is in an amount effective to ameliorate at least one symptom of the neoplastic disorder, for example, reduced cell proliferation or reduced tumor mass, among others, in a subject in need of such treatment.
Compounds of the application have been demonstrated to inhibit HPK1 and hence cytokine release in immune derived cell (e.g. Jurkat-T cells). Therefore in another embodiment of the present application, the disease, disorder or condition that is treatable by inhibiting HPK1 is cancer. Accordingly, the present application also includes a method of treating cancer comprising administering a therapeutically effective amount of one or more compounds of the application to a subject in need thereof. The present application also includes a use of one or more compounds of the application for treatment of cancer as well as a use of one or more compounds of the application for the preparation of a medicament for treatment of cancer. The application further includes one or more compounds of the application for use in treating cancer. In an embodiment, the compound is administered for the prevention of cancer in a subject such as a mammal having a predisposition for cancer.
In some embodiments, the cancer is selected from, but not limited to: Acute Lymphoblastic Leukemia, Adult; Acute Lymphoblastic Leukemia, Childhood; Acute Myeloid Leukemia, Adult; Adrenocortical Carcinoma; Adrenocortical Carcinoma, Childhood; AIDS-Related Lymphoma; AIDS-Related Malignancies; Anal Cancer; Astrocytoma, Childhood Cerebellar; Astrocytoma, Childhood Cerebral; Bile Duct Cancer, Extrahepatic; Bladder Cancer; Bladder Cancer, Childhood; Bone Cancer, Osteosarcoma/Malignant Fibrous Histiocytoma; Brain Stem Glioma, Childhood; Brain Tumor, Adult; Brain Tumor, Brain Stem Glioma, Childhood; Brain Tumor, Cerebellar Astrocytoma, Childhood; Brain Tumor, Cerebral Astrocytoma/Malignant Glioma, Childhood; Brain Tumor, Ependymoma, Childhood; Brain Tumor, Medulloblastoma, Childhood; Brain Tumor, Supratentorial Primitive Neuroectodermal Tumors, Childhood; Brain Tumor, Visual Pathway and Hypothalamic Glioma, Childhood; Brain Tumor, Childhood (Other); Breast Cancer; Breast Cancer and Pregnancy; Breast Cancer, Childhood; Breast Cancer, Male; Bronchial Adenomas/Carcinoids, Childhood; Carcinoid Tumor, Childhood; Carcinoid Tumor, Gastrointestinal; Carcinoma, Adrenocortical; Carcinoma, Islet Cell; Carcinoma of Unknown Primary; Central Nervous System Lymphoma, Primary; Cerebellar Astrocytoma, Childhood; Cerebral Astrocytoma/Malignant Glioma, Childhood; Cervical Cancer; Childhood Cancers; Chronic Lymphocytic Leukemia; Chronic Myelogenous Leukemia; Chronic Myeloproliferative Disorders; Clear Cell Sarcoma of Tendon Sheaths; Colon Cancer; Colorectal Cancer, Childhood; Cutaneous T-Cell Lymphoma; Endometrial Cancer; Ependymoma, Childhood; Epithelial Cancer, Ovarian; Esophageal Cancer; Esophageal Cancer, Childhood; Ewing's Family of Tumors; Extracranial Germ Cell Tumor, Childhood; Extragonadal Germ Cell Tumor; Extrahepatic Bile Duct Cancer; Eye Cancer, Intraocular Melanoma; Eye Cancer, Retinoblastoma; Gallbladder Cancer; Gastric (Stomach) Cancer; Gastric (Stomach) Cancer, Childhood; Gastrointestinal Carcinoid Tumor; Germ Cell Tumor, Extracranial, Childhood; Germ Cell Tumor, Extragonadal; Germ Cell Tumor, Ovarian; Gestational Trophoblastic Tumor; Glioma, Childhood Brain Stem; Glioma, Childhood Visual Pathway and Hypothalamic; Hairy Cell Leukemia; Head and Neck Cancer; Hepatocellular (Liver) Cancer, Adult (Primary); Hepatocellular (Liver) Cancer, Childhood (Primary); Hodgkin's Lymphoma, Adult; Hodgkin's Lymphoma, Childhood; Hodgkin's Lymphoma During Pregnancy; Hypopharyngeal Cancer; Hypothalamic and Visual Pathway Glioma, Childhood; Intraocular Melanoma; Islet Cell Carcinoma (Endocrine Pancreas); Kaposi's Sarcoma; Kidney Cancer; Laryngeal Cancer; Laryngeal Cancer, Childhood; Leukemia, Acute Lymphoblastic, Adult; Leukemia, Acute Lymphoblastic, Childhood; Leukemia, Acute Myeloid, Adult; Leukemia, Acute Myeloid, Childhood; Leukemia, Chronic Lymphocytic; Leukemia, Chronic Myelogenous; Leukemia, Hairy Cell; Lip and Oral Cavity Cancer; Liver Cancer, Adult (Primary); Liver Cancer, Childhood (Primary); Lung Cancer, Non-Small Cell; Lung Cancer, Small Cell; Lymphoblastic Leukemia, Adult Acute; Lymphoblastic Leukemia, Childhood Acute; Lymphocytic Leukemia, Chronic; Lymphoma, AIDS-Related; Lymphoma, Central Nervous System (Primary); Lymphoma, Cutaneous T-Cell; Lymphoma, Hodgkin's, Adult; Lymphoma, Hodgkin's, Childhood; Lymphoma, Hodgkin's During Pregnancy; Lymphoma, Non-Hodgkin's, Adult; Lymphoma, Non-Hodgkin's, Childhood; Lymphoma, Non-Hodgkin's During Pregnancy; Lymphoma, Primary Central Nervous System; Macroglobulinemia, Waldenstrom's; Male Breast Cancer; Malignant Mesothelioma, Adult; Malignant Mesothelioma, Childhood; Malignant Thymoma; Medulloblastoma, Childhood; Melanoma; Melanoma, Intraocular; Merkel Cell Carcinoma; Mesothelioma, Malignant; Metastatic Squamous Neck Cancer with Occult Primary; Multiple Endocrine Neoplasia Syndrome, Childhood; Multiple Myeloma/Plasma Cell Neoplasm; Mycosis Fungoides; Myelodysplastic Syndromes; Myelogenous Leukemia, Chronic; Myeloid Leukemia, Childhood Acute; Myeloma, Multiple; Myeloproliferative Disorders, Chronic; Nasal Cavity and Paranasal Sinus Cancer; Nasopharyngeal Cancer; Nasopharyngeal Cancer, Childhood; Neuroblastoma; Non-Hodgkin's Lymphoma, Adult; Non-Hodgkin's Lymphoma, Childhood; Non-Hodgkin's Lymphoma During Pregnancy; Non-Small Cell Lung Cancer; Oral Cancer, Childhood; Oral Cavity and Lip Cancer; Oropharyngeal Cancer; Osteosarcoma/Malignant Fibrous Histiocytoma of Bone; Ovarian Cancer, Childhood; Ovarian Epithelial Cancer; Ovarian Germ Cell Tumor; Ovarian Low Malignant Potential Tumor; Pancreatic Cancer; Pancreatic Cancer, Childhood; Pancreatic Cancer, Islet Cell; Paranasal Sinus and Nasal Cavity Cancer; Parathyroid Cancer; Penile Cancer; Pheochromocytoma; Pineal and Supratentorial Primitive Neuroectodermal Tumors, Childhood; Pituitary Tumor; Plasma Cell Neoplasm/Multiple Myeloma; Pleuropulmonary Blastoma; Pregnancy and Breast Cancer; Pregnancy and Hodgkin's Lymphoma; Pregnancy and Non-Hodgkin's Lymphoma; Primary Central Nervous System Lymphoma; Primary Liver Cancer, Adult; Primary Liver Cancer, Childhood; Prostate Cancer; Rectal Cancer; Renal Cell (Kidney) Cancer; Renal Cell Cancer, Childhood; Renal Pelvis and Ureter, Transitional Cell Cancer; Retinoblastoma; Rhabdomyosarcoma, Childhood; Salivary Gland Cancer; Salivary Gland Cancer, Childhood; Sarcoma, Ewing's Family of Tumors; Sarcoma, Kaposi's; Sarcoma (Osteosarcoma)/Malignant Fibrous Histiocytoma of Bone; Sarcoma, Rhabdomyosarcoma, Childhood; Sarcoma, Soft Tissue, Adult; Sarcoma, Soft Tissue, Childhood; Sezary Syndrome; Skin Cancer; Skin Cancer, Childhood; Skin Cancer (Melanoma); Skin Carcinoma, Merkel Cell; Small Cell Lung Cancer; Small Intestine Cancer; Soft Tissue Sarcoma, Adult; Soft Tissue Sarcoma, Childhood; Squamous Neck Cancer with Occult Primary, Metastatic; Stomach (Gastric) Cancer; Stomach (Gastric) Cancer, Childhood; Supratentorial Primitive Neuroectodermal Tumors, Childhood; T-Cell Lymphoma, Cutaneous; Testicular Cancer; Thymoma, Childhood; Thymoma, Malignant; Thyroid Cancer; Thyroid Cancer, Childhood; Transitional Cell Cancer of the Renal Pelvis and Ureter; Trophoblastic Tumor, Gestational; Unknown Primary Site, Cancer of, Childhood; Unusual Cancers of Childhood; Ureter and Renal Pelvis, Transitional Cell Cancer; Urethral Cancer; Uterine Sarcoma; Vaginal Cancer; Visual Pathway and Hypothalamic Glioma, Childhood; Vulvar Cancer; Waldenstrom's Macro globulinemia; and Wilms' Tumor. Metastases of the aforementioned cancers can also be treated in accordance with the methods described herein.
In an embodiment, the cancer is selected from hematologic cancers, breast cancers, ovarian cancers, lung cancers, melanomas, colon cancers and glioblastomas.
In an embodiment, the disease, disorder or condition that is treatable by inhibiting HPK1 is a disease, disorder or condition associated with an uncontrolled and/or abnormal cellular activity affected directly or indirectly by inhibiting HPK1. In another embodiment, the uncontrolled and/or abnormal cellular activity that is affected directly or indirectly by inhibiting HPK1 is proliferative activity in a cell. Accordingly, the application also includes a method of inhibiting proliferative activity in a cell, comprising administering an effective amount of one or more compounds of the application to the cell. The present application also includes a use of one or more compounds of the application for inhibition of proliferative activity in a cell as well as a use of one or more compounds of the application for the preparation of a medicament for inhibition of proliferative activity in a cell. The application further includes one or more compounds of the application for use in inhibiting proliferative activity in a cell by boosting immune cell function through HPK1 inhibition.
The present application also includes a method of inhibiting uncontrolled and/or abnormal cellular activities affected directly or indirectly by inhibiting HPK1 in a cell, either in a biological sample or in a subject, comprising administering an effective amount of one or more compounds of the application to the cell. The application also includes a use of one or more compounds of the application for inhibition of uncontrolled and/or abnormal cellular activities affected directly or indirectly by inhibiting HPK1 in a cell as well as a use of one or more compounds of the application for the preparation of a medicament for inhibition of uncontrolled and/or abnormal cellular activities affected directly or indirectly by inhibiting HPK1 in a cell. The application further includes one or more compounds of the application for use in inhibiting uncontrolled and/or abnormal cellular activities affected directly or indirectly by inhibiting HPK1 in a cell.
The present application also includes a method of treating a disease, disorder or condition that is treatable by inhibiting HPK1 comprising administering a therapeutically effective amount of one or more compounds of the application in combination with another known agent useful for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1 to a subject in need thereof. The present application also includes a use of one or more compounds of the application in combination with another known agent useful for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1 for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1, as well as a use of one or more compounds of the application in combination with another known agent useful for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1 for the preparation of a medicament for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1. The application further includes one or more compounds of the application in combination with another known agent useful for treatment of a disease, disorder or condition that is treatable by inhibiting HPK1 for use in treating a disease, disorder or condition that is treatable by inhibiting HPK1. In an embodiment, the disease, disorder or condition treatable by inhibiting HPK1 is cancer.
In a further embodiment, the disease, disorder or condition that is treatable by inhibiting HPK1 is cancer and the one or more compounds of the application are administered in combination with one or more additional cancer treatments. In another embodiment, the additional cancer treatment is selected from radiotherapy, chemotherapy, targeted therapies such as antibody therapies (including anti-PD1 and/or anti-PD-L1 antibodies) and small molecule therapies such as tyrosine-kinase inhibitors and glutaminase inhibitors (GLS1 inhibitors), immunotherapy, hormonal therapy and anti-angiogenic therapies.
When used in combination with other agents or therapies useful in treating diseases, disorders or conditions that are treatable by inhibiting HPK1, it is an embodiment that the compounds of the application are administered contemporaneously with those agents or therapies. As used herein, “contemporaneous administration” of two substances or therapies to a subject means providing each of the two substances or therapies so that they are both biologically active in the individual at the same time. The exact details of the administration will depend on the pharmacokinetics of the two substances or therapies in the presence of each other, and can include administering the two substances or therapies within a few hours of each other, or even administering one substance or therapy within 24 hours of administration of the other, if the pharmacokinetics are suitable. Design of suitable dosing regimens is routine for one skilled in the art. In particular embodiments, the substances or therapies will be administered substantially simultaneously, i.e., within minutes of each other, or in a single composition in the case of administration of two substances. It is a further embodiment of the present application that a combination of agents or therapies is administered to a subject in a non-contemporaneous fashion.
In an embodiment, the subject is a mammal. In an embodiment, the subject is human.
In the context of treating a disease, disorder or condition treatable by inhibition of HPK1, an effective amount is an amount that, for example, inhibits HPK1, compared to the inhibition without administration of the one or more compounds. Effective amounts may vary according to factors such as the disease state, age, sex and/or weight of the subject. The amount of a given compound that will correspond to such an amount will vary depending upon various factors, such as the given drug or compound, the pharmaceutical formulation, the route of administration, the type of condition, disease or disorder, the identity of the subject being treated, and the like, but can nevertheless be routinely determined by one skilled in the art. The effective amount is one that following treatment therewith manifests as an improvement in or reduction of any disease symptom. When the disease is cancer, amounts that are effective can cause a reduction in the number, growth rate, size and/or distribution of tumours.
The dosage of compounds of the application can vary depending on many factors such as the pharmacodynamic properties of the compound, the mode of administration, the age, health and weight of the recipient, the nature and extent of the symptoms, the frequency of the treatment and the type of concurrent treatment, if any, and the clearance rate of the compound in the subject to be treated. One of skill in the art can determine the appropriate dosage based on the above factors. Compounds of the application may be administered initially in a suitable dosage that may be adjusted as required, depending on the clinical response. Dosages will generally be selected to maintain a serum level of compounds of the application from about 0.01 μg/cc to about 1000 μg/cc, or about 0.1 μg/cc to about 100 μg/cc. As a representative example, oral dosages of one or more compounds of the application will range between about 1 mg per day to about 1000 mg per day for an adult, suitably about 1 mg per day to about 500 mg per day, more suitably about 1 mg per day to about 200 mg per day. For parenteral administration, a representative amount is from about 0.001 mg/kg to about 10 mg/kg, about 0.01 mg/kg to about 10 mg/kg, about 0.01 mg/kg to about 1 mg/kg or about 0.1 mg/kg to about 1 mg/kg will be administered. For oral administration, a representative amount is from about 0.001 mg/kg to about 10 mg/kg, about 0.1 mg/kg to about 10 mg/kg, about 0.01 mg/kg to about 1 mg/kg or about 0.1 mg/kg to about 1 mg/kg. For administration in suppository form, a representative amount is from about 0.1 mg/kg to about 10 mg/kg or about 0.1 mg/kg to about 1 mg/kg. In an embodiment of the application, compositions are formulated for oral administration and the compounds are suitably in the form of tablets containing 0.25, 0.5, 0.75, 1.0, 5.0, 10.0, 20.0, 25.0, 30.0, 40.0, 50.0, 60.0, 70.0, 75.0, 80.0, 90.0, 100.0, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950 or 1000 mg of active ingredient per tablet. Compounds of the application may be administered in a single daily, weekly or monthly dose or the total daily dose may be divided into two, three or four daily doses.
Treatment methods comprise administering to a subject a therapeutically effective amount of one or more of the compounds of the application and optionally consist of a single administration, or alternatively comprise a series of administrations, and optionally comprise concurrent administration or use of one or more other therapeutic agents. In an embodiment, the compounds of the application are administered at least once a week. However, in another embodiment, the compounds are administered to the subject from about one time per two weeks, three weeks or one month. In another embodiment, the compounds are administered about one time per week to about once daily. In another embodiment, the compounds are administered 2, 3, 4, 5 or 6 times daily. The length of the treatment period depends on a variety of factors, such as the severity of the disease, disorder or condition, the age of the subject, the concentration and/or the activity of the compounds of the application, and/or a combination thereof. It will also be appreciated that the effective dosage of the compound used for the treatment may increase or decrease over the course of a particular treatment regime. Changes in dosage may result and become apparent by standard diagnostic assays known in the art. In some instances, chronic administration is required. For example, the compounds are administered to the subject in an amount and for duration sufficient to treat the subject.
Compounds of the present application can be prepared by various synthetic processes. The choice of particular structural features and/or substituents may influence the selection of one process over another. The selection of a particular process to prepare a given compound of Formula (I) is within the purview of the person of skill in the art. Some starting materials for preparing compounds of the present application are available from commercial chemical sources. Other starting materials, for example as described below, are readily prepared from available precursors using straightforward transformations that are well known in the art. In the Schemes below showing the preparation of compounds of the application, all variables are as defined in Formula I, unless otherwise stated.
The compounds of Formula (I) generally can be prepared according to the processes illustrated in the Schemes below. In the structural formulae shown below the variables are as defined in Formula (I) unless otherwise stated. A person skilled in the art would appreciate that many of the reactions depicted in the Schemes below would be sensitive to oxygen and water and would know to perform the reaction under an anhydrous, inert atmosphere if needed. Reaction temperatures and times are presented for illustrative purposes only and may be varied to optimize yield as would be understood by a person skilled in the art.
Throughout the processes described herein it is to be understood that, where appropriate, suitable protecting groups will be added to, and subsequently removed from, the various reactants and intermediates in a manner that will be readily understood by one skilled in the art. Conventional procedures for using such protecting groups as well as examples of suitable protecting groups are described, for example, in “Protective Groups in Organic Synthesis”, T. W. Green, P. G. M. Wuts, Wiley-Interscience, New York, (1999).
It is also to be understood that a transformation of a group or substituent into another group or substituent by chemical manipulation can be conducted on any intermediate or final product on the synthetic path toward the final product, in which the possible type of transformation is limited only by inherent incompatibility of other functionalities carried by the molecule at that stage to the conditions or reagents employed in the transformation. Such inherent incompatibilities, and ways to circumvent them by carrying out appropriate transformations and synthetic steps in a suitable order, will be readily understood to one skilled in the art. Examples of transformations are given herein, and it is to be understood that the described transformations are not limited only to the generic groups or substituents for which the transformations are exemplified. References and descriptions of other suitable transformations are given in “Comprehensive Organic Transformations—A Guide to Functional Group Preparations” R. C. Larock, VHC Publishers, Inc. (1989). References and descriptions of other suitable reactions are described in textbooks of organic chemistry, for example, “Advanced Organic Chemistry”, March, 4th ed. McGraw Hill (1992) or, “Organic Synthesis”, Smith, McGraw Hill, (1994).
Techniques for purification of intermediates and final products include, for example, straight and reversed phase chromatography on column or rotating plate, recrystallisation, distillation and liquid-liquid or solid-liquid extraction, which will be readily understood by one skilled in the art.
The products of the processes of the application may be isolated according to known methods, for example, the compounds may be isolated by evaporation of the solvent, by filtration, centrifugation, chromatography or other suitable method.
Generally, the reactions described above are performed in a suitable inert organic solvent and at temperatures and for times that will optimize the yield of the desired compounds. Examples of suitable inert organic solvents include, but are not limited to, 2-propanol, dimethylformamide (DMF), 1,4-dioxane, methylene chloride, chloroform, tetrahydrofuran (THF), toluene, and the like.
The formation of a desired compound salt is achieved using standard techniques. For example, the neutral compound is treated with an acid or base in a suitable solvent and the formed salt is isolated by filtration, extraction or any other suitable method.
The formation of solvates of the compounds of the application will vary depending on the compound and the solvate. In general, solvates are formed by dissolving the compound in the appropriate solvent and isolating the solvate by cooling or using an antisolvent. The solvate is typically dried or azeotroped under ambient conditions. The selection of suitable conditions to form a particular solvate can be made by a person skilled in the art.
Prodrugs of the compounds of the present application may be, for example, conventional esters formed with available hydroxy, thiol, amino or carboxyl groups. For example, available hydroxy or amino groups may be acylated using an activated acid in the presence of a base, and optionally, in inert solvent (e.g. an acid chloride in pyridine).
One skilled in the art will recognize that where a reaction step of the present application is carried out in a variety of solvents or solvent systems, said reaction step may also be carried out in a mixture of the suitable solvents or solvent systems.
Accordingly, in an embodiment, the compounds of Formula (I) are prepared as shown in Scheme 1.
Accordingly, aminopyridine compounds of Formula (A) are mono-halogenated to form compounds of Formula (B), followed by subsequent halogenation to form intermediate compounds of Formula (C) wherein Hal1 and Hal2 are independently a halogen. Intermediate (C) is then coupled to boronic acid or boronic ester intermediate compounds of Formula (D) to form aminopyridine intermediate compounds of Formula (E). In an embodiment Hal1 and Hal2 are different halogens selected to have differing reactivity in the coupling reactions as would be known to those skilled in the art. In an embodiment Hal1 and Hal2 are Br and Cl respectively. In an embodiment Hal1 and Hal2 are I and Cl respectively. In an embodiment Hal1 and Hal2 are I and Br respectively. In an embodiment, Ra and Rb or Rc and Rd are all H. In an embodiment, Ra and Rb or Rc and Rd form a cycloalkyl ring. Compounds of Formula (E) are then coupled to boronic acid or ester compounds of Formula (F) to form the compounds of Formula (I). The variables R1, R2, Q, X1, X2, X3, Cy1 are as defined in Formula (I). In an embodiment, both coupling reactions are performed under cross-coupling conditions, such as in the presence of a cross-coupling catalyst and in an inert solvent. In some embodiments the cross-coupling catalyst is a palladium catalyst. In an embodiment the halogenation conditions comprise a halogenation reagent, such as N-bromosuccinamide.
In a further embodiment, the compounds of Formula (I) are synthesized as shown in Scheme 2 by first coupling a boronic acid or a boronic ester compound of Formula (F) with a dihalogenated intermediate compound of Formula (G) by a Suzuki-Miyaura reaction to form the intermediate compound of Formula (H). Intermediate compound of Formula (H) is then reacted with a suitable boronic acid or boronic ester intermediate compound of Formula (D) to form a compound of Formula (I). The variables R1, R2, Q, X1, X2, X3, Cy1 are as defined in Formula (I). In an embodiment Hal1 and Hal2 are different halogens selected to have differing reactivity in the coupling reactions as would be known to those skilled in the art. In an embodiment of the application, Hal1 and Hal2 are Br and I respectively. In an embodiment, Ra and Rb or Rc and Rd are all H. In an embodiment, Ra and Rb or Rc and Rd together form a cycloalkyl ring. In an embodiment, both coupling reactions are performed under cross-coupling conditions, such as in the presence of a cross-coupling catalyst and in an inert solvent. In some embodiments the cross-coupling catalyst is a palladium catalyst.
In a further embodiment, the compounds of Formula (C) are synthesized as shown in Scheme 3. Therefore, compounds of Formula (C) can be prepared by treating intermediate compound of Formula (A) with a halogenating reagent such as NBS to form mono-halogenated intermediate compound of Formula B which is then treated with a halogenating reagent such as N-iodosuccinimide (NIS) to provide the compounds of Formula C. In an embodiment Hal1 and Hal2 are different halogens selected to have differing reactivity in the coupling reactions as would be known to those skilled in the art. In an embodiment, Hal1 and Hal2 are, for example, Br and I respectively.
In an embodiment, the compounds of Formula (F) are synthesized as shown in Scheme 4. Therefore, compounds of Formula (F) are prepared by sequentially treating intermediate compounds of Formula (H) wherein Hal is a halogen with a suitable borylating agent such as 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane in the presence of a suitable catalyst such as [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) dichloromethane in an inert solvent such as 1,4-dixoane.
In an embodiment, as shown in Scheme 5, compounds of Formula (I) wherein Cy1 is phenyl, and R9 is
wherein R13a is H or R13 are prepared by coupling a boronic acid or boronic ester compound of Formula (J) with a halogenated compound of Formula (K) under suitable conditions, for example, under Suzuki coupling conditions, to form compounds of Formula (I). In an embodiment, R9 is an amino protecting group, for example, tert-butyloxycarbonyl (Boc) which is removed under suitable conditions, for example, with strong acids such as trifluoroacetic acid to form a compound of Formula (I) wherein R9a is H. In an embodiment, Hal2 is Br. In an embodiment, Ri and Rj are both H. In an embodiment Ri and Rj together, form a cycloalkyl ring. The variables, Q, X1, X2, X3, Cy1 and Cy2 are as defined in Formula (I). In an embodiment, the compound of Formula I is the S-enantiomer, R7a is the S-enantionmer,
In an embodiment, as shown in Scheme 6, the intermediate compound of Formula (K) in Scheme 5 is synthesized by coupling dihalogenated ester compound of Formula (L) with halogened compound of Formula (M) under suitable coupling conditions such as in the presence of zinc to form halogenated ester compound of Formula (N). The halogenated ester compound of Formula (N) is then reduced under suitable reducing conditions such as in the presence of lithium aluminum hydride to form hydroxy compound of Formula (O) which is subsequently oxidized under suitable oxidizing conditions such as in the presence of manganese dioxide (MnO2) to provide the halogenated aldehyde compound of Formula (P). The compound of Formula (P) is subsequently coupled with tert-butanesulfinamide (compound of Formula (Q)) to form the aldimine compound of Formula (R) which is further coupled with a (1,3-dioxan-2-ylethyl) (1,3-dioxan-2-ylethyl)magnesium bromide (compound of Formula (S)) under suitable Grignard reaction conditions to provide intermediate compound of Formula (T) which is cyclized under suitable cyclization conditions such as in the presence of trifluoric acid (TFA) and triethylsilane (EtSiH) to form the compound of Formula (K). In an embodiment, the tert-butanesulfinamide (compound of Formula Q) is S-tert-butanesulfinamide and the subsequent compound of Formula (R), (T), and (K) are S-enantiomers. In an embodiment Hal3, Hal4 and Hal5 are each halogens selected to work in the specific coupling reaction as would be known to those skilled in the art. In an embodiment Hal3 and Hal4 are Br and I respectively. In an embodiment, Hal5 is any suitable halogen. In an embodiment, Hal5 is I. The variables Cy1 and Cy2 are as defined in Formula I.
The schemes above are provided for illustration purposes. It will be understood by a person skilled in the art that the use of the most appropriate reagents may vary depend upon of the intermediates compounds of Formula (A)-(T), and that the most appropriate route may also be dependent upon the intermediates and the target compound of Formula (I).
Intermediates of compounds of Formula (A)-(T) are either commercially available or may be prepared using methods known in the art.
Generally the reactions described above are performed in a suitable inert organic solvent and at temperatures and for times that will optimize the yield of the desired compounds. Examples of suitable inert organic solvents include, but are not limited to, dimethylformamide (DMF), dioxane, methylene chloride, chloroform, tetrahydrofuran (THF), toluene, and the like.
Salts of the compounds of the application are generally formed by dissolving the neutral compound in an inert organic solvent and adding either the desired acid or base and isolating the resulting salt by either filtration or other known means.
The formation of solvates of the compounds of the application will vary depending on the compound and the solvate. In general, solvates are formed by dissolving the compound in the appropriate solvent and isolating the solvate by cooling or using an antisolvent. The solvate is typically dried or azeotroped under ambient conditions. The selection of suitable conditions to form a particular solvate can be made by a person skilled in the art. Examples of suitable solvents are ethanol, water and the like. When water is the solvent, the molecule is referred to as a “hydrate”.
Prodrugs of the compounds of the present application may be, for example, conventional esters formed with available hydroxy, thiol, amino or carboxyl groups. For example, available hydroxy or amino groups may be acylated using an activated acid in the presence of a base, and optionally, in inert solvent (e.g. an acid chloride in pyridine). Some common esters which have been utilized as prodrugs are phenyl esters, aliphatic (C1-C24) esters, acyloxymethyl esters, carbamates and amino acid esters.
Compounds of the application, or pharmaceutically acceptable salts, solvates and/or prodrug thereof, which comprise deuterium can be prepared according to the processes illustrated in the schemes above, with deuterium being incorporated through commercially available deuterated alkylating agents, by reductions using deuterium analogs of common reducing agents and/or using iminium chemistry through known methods, for example, using the synthetic procedures found in Zhan et al., J. Label Compd. Radiopharm 2014, 57 533-539. Further isotopically-enriched compounds of the application or pharmaceutically acceptable salts, solvates and/or prodrug thereof, can be prepared using conventional techniques well known to those skilled in the art or by processes analogous to those described in the Schemes and Examples herein using suitable isotopically-enriched reagents and/or intermediates.
Throughout the processes described herein it is to be understood that, where appropriate, suitable protecting groups will be added to, and subsequently removed from, the various reactants and intermediates in a manner that will be readily understood by one skilled in the art. Conventional procedures for using such protecting groups as well as examples of suitable protecting groups are described, for example, in “Protective Groups in Organic Synthesis”, T. W. Green, P. G. M. Wuts, Wiley-Interscience, New York, (1999). It is also to be understood that a transformation of a group or substituent into another group or substituent by chemical manipulation can be conducted on any intermediate or final product on the synthetic path toward the final product, in which the possible type of transformation is limited only by inherent incompatibility of other functionalities carried by the molecule at that stage to the conditions or reagents employed in the transformation. Such inherent incompatibilities, and ways to circumvent them by carrying out appropriate transformations and synthetic steps in a suitable order, will be readily understood to one skilled in the art. Examples of transformations are given herein, and it is to be understood that the described transformations are not limited only to the generic groups or substituents for which the transformations are exemplified. References and descriptions of other suitable transformations are given in “Comprehensive Organic Transformations—A Guide to Functional Group Preparations” R. C. Larock, VHC Publishers, Inc. (1989). References and descriptions of other suitable reactions are described in textbooks of organic chemistry, for example, “Advanced Organic Chemistry”, March, 4th ed. McGraw Hill (1992) or, “Organic Synthesis”, Smith, McGraw Hill, (1994). Techniques for purification of intermediates and final products include, for example, straight and reversed phase chromatography on column or rotating plate, recrystallisation, distillation and liquid-liquid or solid-liquid extraction, which will be readily understood by one skilled in the art.
The following non-limiting examples are illustrative of the present application.
Step 1: 3-Chloro-4-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine
In a microwave vial with magnetic stir bar was placed 5-bromo-3-chloro-4-fluoropyridin-2-amine 1 (208 mg, 0.92 mmol), 4-(4-methylpiperazin-1-yl)phenylboronic acid, pinacol ester (307 mg, 1.02 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (Pd(dppf)Cl2), (67.5 mg, 0.092 mmol), Cs2CO3 (902 mg, 2.77 mmol), 1,2-dimethoxyethane (DME) (8 mL) and water (4 mL). The flask was sealed, with a cap, and then heated to 90° C. in the microwave (high absorbance) for 1 h. LCMS analysis indicated complete conversion and the reaction was concentrated directly onto Celite® using a Biotage® V-10 evaporator and then purified by silica gel chromatography (eluting with CH2Cl2/MeOH), followed by reverse phase chromatography (C18, H2O-MeCN) to provide the title compound (288 mg, 0.90 mmol) in 97% yield as a tan solid. LCMS: [M+H]+=321.25.
Step 2 (General Method A): 6-(2-amino-4-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
In a microwave vial with magnetic stir bar was placed XPhos Pd G2 (2.94 mg, 3.74 μmol), 3-chloro-4-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (60 mg, 0.187 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (77 mg, 0.281 mmol), butan-1-ol (1.200 mL) and potassium phosphate tribasic reagent grade, >=98% (0.288 mL, 0.374 mmol) (4:1 BuOH/H2O). The flask was sealed and flushed with nitrogen, then heated to 90° C. for 1 h in the microwave when LCMS indicated complete conversion. The reaction was then concentrated and the crude material was loaded onto Celite® and purified by silica gel chromatography (eluting with DCM-MeOH) to give the crude product. This material was then purified through a PoraPak® RxnCX 6 cc column (acidic PE resin), eluting with 3% NH4OH in MeOH to give the title compound (15 mg, 19%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.06 (d, J=10.9 Hz, 1H), 7.97 (br s, 1H), 7.94 (d, J=7.8 Hz, 1H), 7.38-7.31 (m, 4H), 6.99 (d, J=8.9 Hz, 2H), 3.41 (td, J=6.5, 2.8 Hz, 2H), 3.18-3.14 (m, 4H), 2.95 (t, J=6.6 Hz, 2H), 2.46-2.43 (m, 4H), 2.22 (s, 3H); LCMS: [M+H]+=432.39.
Step 1: 3-Chloro-4-fluoro-5-(4-morpholinophenyl)pyridin-2-amine
The procedure followed was similar to that of Example 1, Step 1 using 5-bromo-3-chloro-4-fluoropyridin-2-amine (212 mg, 0.94 mmol) and 4-morpholinophenylboronic acid, pinacol ester (299 mg, 1.03 mmol) to provide the title compound (194 mg, 67%) as a grey solid.
Step 2: 6-(2-amino-4-fluoro-5-(4-morpholinophenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
The procedure followed was similar to Example 1, step 2 using XPhos Pd G2 (2.94 mg, 3.74 μmol), 3-chloro-4-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (60 mg, 0.187 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (77 mg, 0.281 mmol), and aqueous K3PO4 (0.288 mL of a 1.3 M solution, 0.374 mmol) and 4:1 BuOH/H2O (1.5 mL). Purification using silica gel chromatography (eluting with DCM-MeOH) gave material that was then purified through a PoraPak® RxnCX 6 cc column (acidic polyethylene (PE) resin), eluting with 3% NH4OH in MeOH to afford the title compound (18 mg, 19%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.06 (d, J=10.9 Hz, 1H), 7.97 (br s, 1H), 7.94 (d, J=7.8 Hz, 1H), 7.38-7.31 (m, 4H), 6.99 (d, J=8.9 Hz, 2H), 3.41 (td, J =6.5, 2.8 Hz, 2H), 3.18-3.14 (m, 4H), 2.95 (t, J=6.6 Hz, 2H), 2.46-2.43 (m, 4H), 2.22 (s, 3H); LCMS: [M+H]+=432.39.
Step 1: 3-chloro-4-fluoro-5-(4-(isopropylsulfonyl)phenyl)pyridin-2-amine
A procedure similar to Example 1, Step 1 was employed using 5-bromo-3-chloro-4-fluoropyridin-2-amine (210 mg, 0.93 mmol) and 4-isopropylsulfonylphenylboronic acid, pinacol ester (318 mg, 1.03 mmol) to provide the title compound (247 mg, 81%) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ 8.19 (d, J=10.0 Hz, 1H), 7.90 (d, J=8.4 Hz, 2H), 7.80 (d, J=7.5 Hz, 2H), 6.97 (br s, 2H), 3.46 (dt, J=13.6, 6.8 Hz, 1H), 1.18 (d, J=6.8 Hz, 6H); LCMS: [M+H]+=329.15.
Step 2: 6-(2-amino-4-fluoro-5-(4-(isopropylsulfonyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
In a microwave vial with a magnetic stir bar was placed 3-chloro-4-fluoro-5-(4-(isopropylsulfonyl)phenyl)pyridin-2-amine (91 mg, 0.277 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (113 mg, 0.415 mmol), XPhos Pd G2 (10.89 mg, 0.014 mmol), Cs2CO3 (271 mg, 0.830 mmol), 1,2-dimethoxyethane (DME) (4 mL) and water (2 mL). The flask was sealed, then heated to 90° C. for 1 h in a microwave apparatus (high absorbance). The reaction then was loaded onto Celite® and purified by reverse phase chromatography (C18, MeCN—H2O) to give the final product (57 mg, 45%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.25 (d, J=10.8 Hz, 1H), 7.99 (br s, 1H), 7.95 (d, J=7.7 Hz, 1H), 7.91-7.87 (m, 2H), 7.81 (d, J=7.9 Hz, 2H), 7.41-7.37 (m, 2H), 6.22 (br s, 2H), 3.48-3.44 (m, 1H), 3.44-3.40 (m, 2H), 2.96 (br t, J=6.5 Hz, 2H), 1.18 (d, J=6.7 Hz, 6H); LCMS: [M+H]+=440.18.
Step 1: (R)-4-(4-chlorophenyl)-2-isopropylmorpholine
A 30 mL vial was charged with 1-chloro-4-iodobenzene (0.50 g, 2.1 mmol), (R)-2-isopropylmorpholine (0.30 g, 2.3 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.06 g, 0.06 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.11 g, 0.19 mmol) and CS2CO3 (2.05 g, 6.3 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (7 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 100° C. for 18 h, cooled to room temperature (RT) and partitioned between EtOAc and water. The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (1-15% EtOAc/hexanes) afforded the product (0.40 g, 79%). LCMS: [M +H]+=240.1.
Step 2: (R)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine
A 30 mL vial was charged with (R)-4-(4-chlorophenyl)-2-isopropylmorpholine (0.40 g, 1.7 mmol), bis(pinacolato)diboron (0.53 g, 2.1 mmol), K2CO3 (0.33 g, 3.3 mmol) and XPhos Pd G2 (0.10 g, 0.12 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (5 mL) was added and the vial was evacuated and backfilled an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (1-15% EtOAc/hexanes) to afford the product (0.45 g, 82%). LCMS: [M+H]+=332.1.
Step 3: (R)-3-chloro-4-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-4-fluoropyridin-2-amine (0.030 g, 0.13 mmol), (R)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine (0.050 g, 0.15 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (10 mg, 0.013 mmol) and cesium carbonate (0.13 g, 0.40 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and H2O (0.5 mL) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 0.5%-6% MeOH/DCM+0.5% NH4OH) to afford the product (0.046 g, quantitative yield). LCMS: [M+H]+=350.5.
Step 4: (R)-6-(2-amino-4-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with (R)-3-chloro-4-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.050 g, 0.14 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.046 g, 0.17 mmol) and XPhos Pd G2 (0.011 g, 0.014 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and aqueous K3PO4 (0.27 mL of a 1.3 M solution, 0.35 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated conventionally at 90° C. for 18 h. After cooling to RT, the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 0.5-7.5% MeOH/DCM+0.5% NH4OH) followed by reverse phase chromatography (C18; 5-60% MeCN/water+0.1% formic acid) to afford the title compound (0.023 g, 36%). 1H NMR (500 MHz, DMSO-d6) δ 8.07 (d, J=10.8 Hz, 1H), 7.9-8.0 (m, 2H), 7.3-7.4 (m, 4H), 7.02 (d, J=8.8 Hz, 2H), 5.85 (s, 2H), 3.9-4.0 (m, 1H), 3.5-3.6 (m, 4H), 3.42 (dt, J=6.5, 2.7 Hz, 3H), 3.24 (ddd, 2H, J=10.3, 6.3, 2.2 Hz), 2.95 (br t, J =6.5 Hz, 2H), 2.67 (dt, J=11.8, 3.4 Hz, 1H), 2.4-2.5 (m, 2H), 1.7-1.8 (m, 1H), 0.96 (dd, J=6.7, 4.4 Hz, 6H); LCMS: [M+H]+=461.7.
Step 1: (S)-4-(4-chlorophenyl)-2-isopropylmorpholine
A 30 mL vial was charged with 1-chloro-4-iodobenzene (0.50 g, 2.1 mmol), (S)-2-isopropylmorpholine (0.30 g, 2.3 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.06 g, 0.06 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.11 g, 0.19 mmol) and Cs2CO3 (2.05 g, 6.3 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (7 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 100° C. for 18 h. The reaction mixture was cooled to RT and partitioned between EtOAc and water. The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (eluting with 1-15% EtOAc/hexanes) afforded the product (0.40 g, 80%). LCMS: [M+H]+=240.0.
Step 2: (S)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine
A 30 mL vial was charged with (S)-4-(4-chlorophenyl)-2-isopropylmorpholine (0.40 g, 1.7 mmol), bis(pinacolato)diboron (0.53 g, 2.1 mmol), KOAc (0.33 g, 3.3 mmol) and XPhos Pd G2 (0.10 g, 0.12 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (5 mL) was added and the vial was evacuated and backfilled an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 1-15% EtOAc/hexanes) to afford the product (0.47 g, 85%). LCMS: [M+H]+=332.1.
Step 3: (S)-3-chloro-4-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-4-fluoropyridin-2-amine (0.030 g, 0.13 mmol), (S)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine (0.050 g, 0.15 mmol), [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (10 mg, 0.013 mmol) and CS2CO3 (0.13 g, 0.40 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and H2O (0.5 mL) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 0.5%-6% MeOH/DCM+0.5% NH4OH) to afford the product (0.052 g, quantitative yield). LCMS: [M+H]+=350.4.
Step 4: (S)-6-(2-amino-4-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with (S)-3-chloro-4-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.052 g, 0.15 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.049 g, 0.18 mmol) and XPhos Pd G2 (0.012 g, 0.015 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and aqueous K3PO4 (0.29 mL of a 1.3 M solution, 0.35 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 0.5-7.5% MeOH/DCM+0.5% NH4OH) followed by reverse phase chromatography (C18; 5-60% MeCN/water +0.1% formic acid) to afford the title compound (29 mg, 42%). 1H NMR (500 MHz, DMSO-d6) δ 8.07 (d, J=10.8 Hz, 1H), 7.9-8.0 (m, 2H), 7.3-7.4 (m, 4H), 7.02 (d, J=8.8 Hz, 2H), 5.85 (s, 2H), 3.9-4.0 (m, 1H), 3.5-3.6 (m, 4H), 3.42 (dt, J=6.5, 2.7 Hz, 3H), 3.24 (ddd, J=10.3, 6.3, 2.2 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.67 (dt, J=11.8, 3.4 Hz, 1H), 2.4-2.5 (m, 2H), 1.7-1.8 (m, 1H), 0.96 (dd, J=6.7, 4.4 Hz, 6H); LCMS: [M+H]+=461.7.
Step 1: (R)-4-(4-chloro-3-fluorophenyl)-2-isopropylmorpholine
A30 mL vial was charged with 4-bromo-1-chloro-2-fluorobenzene (0.50 g, 2.4 mmol), (R)-2-isopropylmorpholine (0.34 g, 2.6 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.066 g, 0.072 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.12 g, 0.22 mmol) and Cs2CO3 (2.3 g, 7.2 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (6 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated conventionally at 100° C. for 18 h. The reaction mixture was cooled to RT and partitioned between EtOAc and water. The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (5% EtOAc/hexanes) afforded the product (0.67 g, quantitative yield). LCMS: [M+H]+=258.1.
Step 2: (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine
A 30 mL vial was charged with (R)-4-(4-chloro-3-fluorophenyl)-2-isopropylmorpholine (0.67 g, 2.6 mmol), bis(pinacolato)diboron (0.83 g, 3.3 mmol), KOAc (0.51 g, 5.2 mmol) and XPhos Pd G2 (0.15 g, 0.20 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (8 mL) was added and the vial was evacuated and backfilled an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 1-15% EtOAc/hexanes) to afford the product (0.91 g, quantitative yield). LCMS: [M+H]+=350.2.
Step 3: (R)-3-chloro-4-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-4-fluoropyridin-2-amine (0.030 g, 0.13 mmol), (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine (0.051 g, 0.15 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (10 mg, 0.013 mmol) and cesium carbonate (0.13 g, 0.40 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and H2O (0.5 mL) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto elite and purified by silica gel chromatography (eluting with 0.5%-6% MeOH/DCM+0.5% NH4OH) to afford the product (0.030 g, 61%). LCMS: [M+H]+=368.6.
Step 4: (R)-6-(2-amino-4-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with (R)-3-chloro-4-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.030 g, 0.082 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.027 g, 0.098 mmol) and XPhos Pd G2 (7 mg, 8.1 μmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and 1.3 M aqueous K3PO4 (0.16 mL of a 1.3 M solution, 0.21 mmol) were added and the reaction vial was evacuated and backfilled with N2 an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 0.5-7.5% MeOH/DCM+0.5% NH4OH) followed by reverse phase chromatography (C18; 5-60% MeCN/water+0.1% formic acid) to afford the title compound (14 mg, 36%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 3H), 7.34 (br s, 2H), 7.2-7.3 (m, 1H), 6.8-6.9 (m, 2H), 5.94 (br s, 2H), 3.9-4.0 (m, 2H), 3.6-3.6 (m, 6H), 3.2-3.3 (m, 2H), 2.95 (br d, J=5.5 Hz, 3H), 2.7-2.7 (m, 2H), 1.7-1.8 (m, 1H), 0.9-1.0 (m, 6H); LCMS [M +H]+=479.5.
Step 1: (S)-4-(4-chloro-3-fluorophenyl)-2-isopropylmorpholine
A 30 mL vial was charged with 4-bromo-1-chloro-2-fluorobenzene (0.50 g, 2.4 mmol), (S)-2-isopropylmorpholine (0.34 g, 2.6 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.066 g, 0.072 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.12 g, 0.22 mmol) and Cs2CO3 (2.3 g, 7.2 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (6 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 100° C. for 18 h, cooled to RT and partitioned between EtOAc and water. The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (eluting with 5% EtOAc/hexanes) afforded the product (0.78 g, quantitative yield). LCMS: [M+H]+=258.2.
Step 2: (S)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine
A 30 mL vial was charged with (S)-4-(4-chloro-3-fluorophenyl)-2-isopropylmorpholine (0.78 g, 3.0 mmol), bis(pinacolato)diboron (0.96 g, 3.8 mmol), KOAc (0.60 g, 6.1 mmol) and XPhos Pd G2 (0.18 g, 0.23 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (8 mL) was added and the vial was evacuated and backfilled an additional time. The reaction was heated at 90° C. for 18 h, cooled to RT and concentrated directly onto Celite®. Purification by flash chromatography (1-15% EtOAc/hexanes) afforded the product (0.81 g, 76%). LCMS: [M+H]+=350.2.
Step 3: (S)-3-chloro-4-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-4-fluoropyridin-2-amine (0.030 g, 0.13 mmol), (S)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine (0.051 g, 0.15 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (10 mg, 0.013 mmol) and cesium carbonate (0.13 g, 0.40 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and Water (0.5 mL) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated conventionally at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 0.5%-6% MeOH/DCM+0.5% NH4OH) to afford the product (0.031 g, 63%). LCMS: [M+H]+=368.5.
Step 4: (S)-6-(2-amino-4-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with (S)-3-chloro-4-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.031 g, 0.084 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.028 g, 0.10 mmol) and XPhos Pd G2 (7 mg, 8.4 μmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and 1.3 M aqueous K3PO4 (0.16 mL, 0.21 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h and allowed to cool to RT. The mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 0.5-7.5% MeOH/DCM+0.5% NH4OH) followed by reverse phase chromatography (C18; 5-60% MeCN/water+0.1% formic acid) to afford the title compound (11 mg, 27%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 3H), 7.34 (br s, 2H), 7.2-7.3 (m, 1H), 6.8-6.9 (m, 2H), 5.94 (br s, 2H), 3.9-4.0 (m, 2H), 3.6-3.6 (m, 5H), 3.2-3.2 (m, 3H), 2.95 (br d, 3H, J=5.7 Hz), 2.7-2.7 (m, 2H), 1.7-1.8 (m, 1H), 0.95 (br d, 6H, J=6.5 Hz); LCMS: [M +H]+=479.7.
Step 1: 5-bromo-6-fluoropyridin-2-amine
To a solution of 6-fluoropyridin-2-amine (3 g, 26.8 mmol) in acetonitrile (100 mL) was added NBS (4.7 g, 26.8 mmol) in acetonitrile (80 mL) dropwise for 30 min at 0° C. and stirred for another 30 min under argon atmosphere at RT. The solvent was evaporated under reduced pressure to give crude product which was purified by column chromatography (silica gel, 100-200 mesh) using 10% EtOAc in pet ether as an eluent to afford the product (4.4 g, 88%) as a yellow solid. LCMS: [M+H]+=191.12.
Step 2: Synthesis of 5-bromo-3-chloro-6-fluoropyridin-2-amine
To a solution of 5-bromo-6-fluoropyridin-2-amine (2 g, 10.5 mmol) in acetonitrile (30 mL) was added NCS (1.4 g, 10.5 mmol) portionwise, and the suspension was refluxed for 2 h at 90° C. under argon atmosphere before cooling to RT. The reaction mixture was poured into ice-cold water (100 mL), and the resulting precipitate was collected to give the crude compound. Washing with n-pentane (100 mL) afforded the product (1.1 g, 48%) as an off-white solid. LCMS: [M+H]+=225.09
Step 3: 3-chloro-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine
In a microwave vial with magnetic stir bar was placed 5-bromo-3-chloro-6-fluoropyridin-2-amine (260 mg, 1.15 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (84 mg, 0.115 mmol), CSCO3 (1127 mg, 3.46 mmol), DME (4 mL) and water (2 mL). The flask was sealed, then heated to 90° C. for 1 h in the microwave (high absorbance) when LCMS indicated complete conversion. The reaction was loaded onto Celite® and purified by reverse phase chromatography (C18, MeCN—H2O) to give the product (0.630 mmol, 55% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.80 (d, J=8.9 Hz, 1H), 7.35 (d, J=7.6 Hz, 2H), 6.96 (d, J=8.9 Hz, 2H), 6.66 (s, 2H), 3.18-3.14 (m, 4H), 2.46-2.42 (m, 4H), 2.22 (s, 3H); LCMS: [M+1]+=323.28.
Step 4: 6-(2-amino-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
In a microwave vial with magnetic stir bar was placed 3-chloro-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (62 mg, 0.194 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (69.5 mg, 0.255 mmol), XPhos Pd G2 (6.68 mg, 8.49 μmol), CSCO3 (166 mg, 0.509 mmol), DME (4 mL) and water (2 mL). The flask was sealed, then heated to 90° C. for 1 h in a microwave (high absorbance) until LCMS indicated complete conversion. The reaction was loaded onto Celite® and purified by reverse phase chromatography (C18, MeCN—H2O) to give the title compound (57 mg, 78%) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ 8.02-7.93 (m, 2H), 7.63 (d, J=10.3 Hz, 1H), 7.54-7.48 (m, 2H), 7.45 (br d, J=7.9 Hz, 2H), 7.02 (br d, J=8.9 Hz, 2H), 6.21 (s, 2H), 3.45 (br dd, J=6.3, 2.3 Hz, 2H), 3.22-3.18 (m, 4H), 3.00 (br t, J=6.4 Hz, 2H), 2.55 (br d, J=1.6 Hz, 2H), 2.27 (s, 3H); LCMS: [M+H]+=432.28.
Step 1: (R)-3-chloro-6-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.040 g, 0.18 mmol, prepared according to Example 8), (R)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine (0.070 g, 0.20 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.014 g, 0.018 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and 2 M aqueous Na2CO3 (0.27 mL, 0.53 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 85° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 5-95% EtOAc/hexanes) to afford the product (0.030 g, 48%). LCMS: [M+H]+=350.4.
Step 2: (R)-6-(2-amino-6-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with (R)-3-chloro-6-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.030 g, 0.086 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.035 g, 0.13 mmol) and XPhos Pd G2 (7 mg, 8.6 μmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and aqueous K3PO4 (0.17 mL of a 1.3 M solution, 0.21 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (0.5-9.5% MeOH/DCM+0.5% NH4OH) to afford the product (0.031 g, 78%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.58 (br d, 1H, J=9.8 Hz), 7.4-7.5 (m, 4H), 7.01 (br d, 2H, J=8.3 Hz), 6.18 (br s, 2H), 3.97 (br d, 1H, J=10.3 Hz), 3.59 (br d, 3H, J=12.5 Hz), 3.53 (br d, 2H, J=11.7 Hz), 3.40 (br d, 4H, J=4.8 Hz), 3.2-3.3 (m, 2H), 2.95 (br s, 2H), 2.66 (br t, 1H, J=11.1 Hz), 2.44 (br t, 1H, J=11.1 Hz), 1.7-1.8 (m, 1H), 0.96 (br s, 6H); LCMS [M+H]+=461.4.
Step 1: (S)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine
A 30 ml vial was charged with (S)-4-(4-chlorophenyl)-2-isopropylmorpholine (0.404 g, 1.685 mmol), Bis(pinacolato)diboron (0.535 g, 2.106 mmol), Potassium acetate (0.331 g, 3.37 mmol) and XPhos Pd G2 (0.099 g, 0.126 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (5 ml) was added and the vial was evacuated and backfilled an additional time. The reaction mixture was then heated to 90° C. in an aluminum block for 18 h. The reaction mixture was concentrated onto celite and purified by flash chromatography (eluting with 5-95% EtOAc/hexanes) to afford the product (0.472 g, 85% yield).
Step 2: (S)-3-chloro-6-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.040 g, 0.177 mmol), (S)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine (0.068 g, 0.204 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.014 g, 0.018 mmol). The vial was sealed with a septum cap, evacuated and backfilled with nitrogen. 1,4-Dioxane (2 ml) and a 2M aqueous solution of saturated aqueous Na2CO3 (0.266 ml, 0.532 mmol) were added and the vial was evacuated and backfilled an additional time. The reaction mixture was heated to 85° C. in an aluminum block for 18 h. LCMS [01] indicated desired product formation. The reaction mixture was concentrated onto celite and purified by flash [5-95% EtOAc/hexanes] to afford the product (28 mg, 45.1% yield). LCMS: [M+H]+=350.36.
Step 3: (S)-6-(2-amino-6-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (I-10)
A 30 mL vial with magnetic stir bar was charged (S)-3-chloro-6-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.028 g, 0.080 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.033 g, 0.120 mmol) and XPhos Pd G2 (6.30 mg, 8.00 μmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (1.5 ml) and aqueous potassium phosphate (0.2 ml) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 85° C. for 18 h in an aluminum block. The reaction mixture was concentrated onto celite and purified by flash chromatography [0.5-9.5% MeOH/DCM+0.5% NH4OH] to afford the title compound (26 mg, 70.5% yield). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.58 (d, 1H, J=10.1 Hz), 7.4-7.5 (m, 4H), 7.01 (br d, 2H, J=8.7 Hz), 6.18 (s, 2H), 3.9-4.0 (m, 1H), 3.6-3.6 (m, 3H), 3.53 (br d, 2H, J=12.1 Hz), 3.40 (br dd, 5H, J=2.2, 6.1 Hz), 3.2-3.3 (m, 2H), 2.95 (br t, 2H, J=6.2 Hz), 2.66 (dt, 1H, J=2.9,11.8 Hz), 2.44 (br t, 1H, J=11.2 Hz), 1.73 (qd, 1H, J=6.6, 13.4 Hz), 0.96 (dd, 6H, J=4.3, 6.7 Hz); LCMS: [M+H]+=461.47.
Step 1: (R)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.040 g, 0.18 mmol, prepared according to Example 8), (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine (0.071 g, 0.20 mmol) and Pd(dppf)Cl2 (0.014 g, 0.018 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and 2 M aqueous Na2CO3 (0.27 mL, 0.53 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 85° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by silica gel chromatography (eluting with 5-95% EtOAc/hexanes) to afford the product (0.039 g, 60% yield). LCMS: [M+H]+=368.3.
Step 2: (R)-6-(2-amino-6-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with (R)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.039 g, 0.11 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.043 g, 0.16 mmol) and XPhos Pd G2 (8 mg, 10 μmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1.5 mL) and 1.3 M aqueous K3PO4 (0.20 mL of a 1.3 M solution, 0.27 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated onto Celite® and purified by silica gel chromatography (eluting with 0.5-9.5% MeOH/DCM+0.5% NH4OH) to afford the title compound (33 mg, 65%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.4-7.5 (m, 3H), 7.29 (br t, J=8.8 Hz, 1H), 6.8-6.9 (m, 2H), 6.27 (br s, 2H), 3.96 (br d, J=10.0 Hz, 1H), 3.5-3.6 (m, 4H), 3.40 (br s, 3H), 3.2-3.3 (m, 2H), 2.94 (br t, J=6.0 Hz, 2H), 2.7-2.7 (m, 1H), 1.7-1.8 (m, 1H), 0.9-1.0 (m, 6H); LCMS: [M+H]+=479.4.
Step 1: (S)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.040 g, 0.18 mmol, prepared according to Example 8), (S)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine (0.071 g, 0.20 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.014 g, 0.018 mmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and 2 M aqueous Na2CO3 (0.27 mL, 0.53 mmol) were added and the reaction vial was evacuated and backfilled with N2 an additional time. The reaction was heated at 85° C. for 18 h. After cooling to RT the reaction mixture was concentrated onto Celite® and purified by silica gel chromatography (eluting with 5-95% EtOAc/hexanes) to afford the desired product (0.036 g, 55%). LCMS: [M+H]+=368.3.
Step 2: (S)-6-(2-amino-6-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with (S)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-2-amine (0.036 g, 0.098 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.040 g, 0.15 mmol) and XPhos Pd G2 (8 mg, 10 μmol). The vial was sealed with a cap and septum and the reaction vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (1.5 mL) and 1.3 M aqueous K3PO4 (0.19 mL, 0.25 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the mixture was concentrated onto Celite® and purified by silica gel chromatography (eluting with 0.5-9.5% MeOH/DCM+0.5% NH4OH) to afford the title compound (0.029 g, 62%). 1 H NMR (500 MHz, DMSO-d6) δ 7.9-7.9 (m, 2H), 7.4-7.5 (m, 3H), 7.2-7.3 (m, 1H), 6.8-6.9 (m, 2H), 6.25 (br s, 2H), 3.9-4.0 (m, 1H), 3.5-3.6 (m, 3H), 3.39 (br d, J=3.4 Hz, 3H), 3.2-3.3 (m, 1H), 2.94 (br d, J=5.7 Hz, 2H), 2.7-2.7 (m, 1H), 1.7-1.8 (m, 1H), 0.9-1.0 (m, 6H); LCMS [M+H]+=479.4.
Step 1: (1R,5S)-3-(4-iodophenyl)-8-methyl-3,8-diazabicyclo[3.2.1]octane
A sealed mixture of 8-methyl-3,8-diaza-bicyclo[3.2.1]octane dihydrochloride (0.802 g, 4.03 mmol) and K3PO4 (2.57 g, 12.09 mmol) in DMF (20 mL) was stirred at RT overnight. The mixture was then degassed with a stream of N2 before Cul (0.154 g, 0.806 mmol), (R)-(+)-1,1′-bi-2-naphthol (0.231 g, 0.806 mmol) and 1,4-diiodobenzene (1.330 g, 4.03 mmol) were added as solids. The degassing step was repeated and the mixture was sealed and heated while stirring at 40° C. for 2 d. The reaction mixture was cooled to RT, diluted with EtOAc and filtered through Celite®. The filtrate was concentrated under reduced pressure and purified by silica gel chromatography (50 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 19% MeOH) to afford the product as a beige solid (310.0 mg, 23%). LCMS: [M+H]+=329.00.
Step 2: 3-chloro-6-fluoro-5-(4-((1R,5S)-8-methyl-3,8-diazabicyclo[3.2.1]octan-3-yl)phenyl)pyridin-2-amine
A procedure similar to General Method A (Example 1, Step 2) using (1R,5S)-3-(4-iodophenyl)-8-methyl-3,8-diazabicyclo[3.2.1]octane (99 mg, 0.300 mmol), Cs2CO3 (293 mg, 0.901 mmol), PdCl2dppf (21.97 mg, 0.030 mmol), H2O (2 mL), 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (90 mg, 0.330 mmol) in 1,4-dioxane (2.7 mL), followed by purification with silica gel chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 6-90% MeOH) afforded the product as a tan solid (93 mg, 80% yield based on purity of 90%). LCMS: [M+H]+=347.35.
Step 3: Synthesis of 6-(2-amino-6-fluoro-5-(4-((1R,5S)-8-methyl-3,8-diazabicyclo[3.2.1]octan-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
The title compound was prepared by a method similar to General Method A (Example 1, Step 2) using XPhos Pd G2 (9.51 mg, 0.012 mmol), 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (39.6 mg, 0.145 mmol), H2O (2 mL), 3-chloro-6-fluoro-5-(4-((1 R,5S)-8-methyl-3,8-diazabicyclo[3.2.1]octan-3-yl)phenyl)pyridin-2-amine (46.6 mg, 0.121 mmol) and MeCN (3 mL); by heating under microwave irradiation at 100° C. for 4 h. Purified by chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 30-100% MeOH) followed by filtration through a Waters PoraPak CX column afforded the title compound as a white solid (18.0 mg, 33%). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.88-7.96 (m, 2H), 7.56 (br d, J=10.2 Hz, 1H), 7.46 (br d, J=8.1 Hz, 1H), 7.44 (s, 1H), 7.37 (br d, J=8.0 Hz, 2H), 6.82-6.86 (m, 2H), 6.10-6.15 (m, 2H), 3.21 (br s, 2H), 2.93-2.98 (m, 2H), 2.84 (br d, J=9.8 Hz, 2H), 2.22 (s, 3H), 1.91-2.00 (m, 2H), 1.58-1.66 (m, 2H). LCMS: [M+H]+=458.46.
Step 1: (1R,5S)-1-(4-bromophenyl)-3-methyl-3-azabicyclo[3.1.0]hexan
To a solution of (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (0.500 g, 2.100 mmol) in MeOH/THF (5 mL each) was added formaldehyde solution, 37% wt in water (0.234 mL, 3.15 mmol) followed by NaBH(OAc)3 (0.668 g, 3.15 mmol). The reaction was stirred at RT for 40 h. The volatiles were removed in vacuo and the residue was partitioned between KOH (1N) and CH2Cl2. The layers were separated and the aqueous layer was extracted with additional CH2Cl2. The combined extracts were dried over Mg2SO4 and concentrated to dryness to afford the product (501 mg, 95%). LCMS: [M+H]+=252.21.
Step 2: 3-chloro-6-fluoro-5-(4-((1R,5S)-3-methyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-2-amine
A procedure similar to Example 1, Step 2 (General Method A) using (1R,5S)-1-(4-bromophenyl)-3-methyl-3-azabicyclo[3.1.0]hexane (0.044 g, 0.174 mmol), PdCl2dppf (0.013 g, 0.017 mmol), Cs2CO3 (0.170 g, 0.523 mmol), H2O (2 mL), 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (57 mg, 0.21 mmol) in 1,4-dioxane (1.74 mL), DME (2 mL), followed by purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 23% MeOH) afforded the product as a brown solid (45 mg, 78% yield based on purity of 96%). LCMS: [M+H]+=318.24.
Step 3: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-methyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, Step 2 (General Method A) using 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one(0.040 g, 0.145 mmol), K3PO4 (0.092 g, 0.435 mmol), 3-chloro-6-fluoro-5-(4-((1 R,5S)-3-methyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-2-amine (0.045 g, 0.142 mmol), H2O (3 mL), MeCN (5 mL), XPhos Pd G2 (0.011 g, 0.015 mmol) and purification by silica gel chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 25% MeOH,), followed by filtration through a Waters PoraPak CX column, afforded the title compound (26 mg, 41% yield based on purity of 98%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 7.88-7.97 (m, 1H), 7.56-7.66 (m, 1H), 7.41-7.51 (m, 4H), 7.18 (br d, J=8.3 Hz, 2H), 6.26 (s, 2H), 3.20-3.32 (m, 1H), 2.93-3.00 (m, 3H), 2.39 (dd, J=8.6, 3.2 Hz, 1H), 2.30 (s, 3H), 1.75-1.84 (m, 1H), 1.32-1.38 (m, 1H), 0.76 (dd, J=7.8, 3.7 Hz, 1H). Multiplets corresponding to two CH2 multiplets are obscured by the solvent and/or H2O peaks. LCMS [M+H]+=429.35.
Step 1. 3-chloro-4-fluoro-5-(4-(isopropylsulfonyl)phenyl)pyridin-2-amine
In a microwave vial with a magnetic stir bar was placed 5-bromo-3-chloro-6-fluoropyridin-2-amine (260 mg, 1.15 mmol, prepared according to Example 9), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (84 mg, 0.115 mmol), Cs2CO3 (1.13 g, 3.46 mmol), 1,2-dimethoxyethane (DME) (4 mL) and H2O (2 mL). The flask was sealed, then heated to 90° C. for 1 h in the microwave until LCMS indicated complete conversion. The reaction was loaded onto Celite® and purified by reverse phase chromatography (C18 column, eluting with MeCN—H2O) to give the product as a brown solid (161 mg, 43%). LCMS: [M+H]+=329.15.
Step 2: 6-(2-amino-6-fluoro-5-(4-(isopropylsulfonyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
In a microwave vial with magnetic stir bar was placed 3-bromo-6-fluoro-5-(4-(isopropylsulfonyl)phenyl)pyridin-2-amine (53 mg, 0.142 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (58.2 mg, 0.213 mmol), XPhos Pd G2 (5.59 mg, 7.10 μmol), Cs2CO3 (139 mg, 0.426 mmol), DME (4 mL) and H2O (2 mL). The flask was sealed, then heated to 90° C. for 1 h in the microwave until LCMS indicated complete conversion. The reaction was loaded onto Celite® and purified by reverse phase chromatography (C18, MeCN—H2O) to give the title compound as a tan solid (49 mg, 78%). 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.92 (br d, J=7.8 Hz, 1H), 7.87 (s, 4H), 7.78 (br d, J=10.1 Hz, 1H), 7.49 (br d, J=7.9 Hz, 1H), 7.46 (s, 1H), 6.55 (br s, 2H), 3.47-3.39 (m, 3H), 2.96 (br t, J=6.2 Hz, 2H), 1.18 (d, J=6.8 Hz, 6H); LCMS: [M+H]+=440.49.
Step 1: 3-chloro-6-fluoro-5-(4-morpholinophenyl)pyridin-2-amine
A procedure similar to that of Example 16, Step 1 using 5-bromo-3-chloro-6-fluoropyridin-2-amine (270 mg, 1.20 mmol, prepared according to Example 8), [1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II) (88 mg, 0.120 mmol), Cs2CO3 (1.17 g, 3.59 mmol), DME (4 mL) and H2O (2 mL) afforded the product (422 mg, 92%). LCMS: [M +H]+=310.20.
Step 2: 6-(2-amino-6-fluoro-5-(4-morpholinophenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
The procedure was similar to Example 16, Step 2 using 3-bromo-6-fluoro-5-(4-morpholinophenyl)pyridin-2-amine (65 mg, 0.185 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (76 mg, 0.277 mmol), XPhos Pd G2 (7.26 mg, 9.23 μmol), Cs2CO3 (180 mg, 0.554 mmol), DME (4 mL) and H2O (2 mL). Workup and purification afforded the title compound as a tan solid (18 mg, 23%). 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.87 (m, 2H), 7.59 (br d, J=10.1 Hz, 1H), 7.49-7.40 (m, 4H), 6.99 (br d, J=8.7 Hz, 2H), 6.18 (br s, 2H), 3.79-3.70 (m, 4H), 3.40 (br d, J=4.2 Hz, 2H), 3.17-3.10 (m, 4H), 2.95 (br t, J=6.2 Hz, 2H); LCMS: [M+H]+=419.31.
Step 1: tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenyl)piperazine-1-carboxylate
A procedure similar to General Procedure A (Example 1) using 5-bromo-3-chloro-6-fluoropyridin-2-amine (300 mg, 1.331 mmol), Cs2CO3 (1301 mg, 3.99 mmol), tert-butyl 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]tetrahydro-1(2H)-pyrazinecarboxylate (672 mg, 1.73 mmol) and [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (117 mg, 0.160 mmol) in DME (15 mL) and H2O (3 mL) afforded the product as a white solid (360 mg, 66%). LCMS: [M+H]+=407.46.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate
A procedure similar to that of Example 1, General Method A using XPhos Pd G2 (30.2 mg, 0.038 mmol) 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (105 mg, 0.383 mmol), tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenyl)piperazine-1-carboxylate (120 mg, 0.295 mmol) and Cs2CO3 (288 mg, 0.885 mmol) in a solvent mixture of DME (10 mL) and H2O (2.5 mL) flushed with argon gas. The mixture was heated at 90° C. in a microwave reactor for 1 h. Standard workup followed by purification using silica gel chromatography (eluting with hexanes/EtOAc followed by 3% MeOH and 0.3% NH4OH in DCM) afforded the desired product as an off white foam (106 mg, judged to be 73% pure), which was taken into the next step without further purification.
Step 3. 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one trifluoroacetic acid salt
The tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate (106 mg, 0.149 mmol) obtained in Step 2 was stirred at RT with a 1:1 mixture of DCM (1.5 ml) and TFA (1 mL) for 10 min. Complete conversion to the desired product was observed. The mixture was concentrated to dryness, passed through a porapak cartridge and purified by reverse phase chromatography (5.5 g column, eluting with water containing 0-10% acetonitrile). The appropriate fractions were combined and concentrated to afford the title compound (62.5 mg, 75% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ=8.80-8.56 (m, 1H), 8.01-7.85 (m, 2H), 7.67-7.56 (m, 1H), 7.51-7.38 (m, 4H), 7.09-6.99 (m, 2H), 6.27-6.16 (m, 2H), 3.43-3.37 (m, 6H), 3.26-3.20 (m, 4H), 2.99-2.91 (m, 2H)19F NMR (471 MHz, DMSO-d6) 5=−73.45 (s, 1F),−73.47 (s, 1F),−75.83 (s, 1F).
Step 1: (1S,4S)-2-methyl-5-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,5-diazabicyclo[2.2.1]heptane
A vial charged with XPhos Pd G2 (21.20 mg, 0.027 mmol), B2pin2 (68.4 mg, 0.269 mmol, KOAc (79 mg, 0.808 mmol, (1S,4S)-2-(4-chlorophenyl)-5-methyl-2,5-diazabicyclo[2.2.1]heptane (60 mg, 0.269 mmol and 1,4-dioxane (6 mL). The vial was sealed and degassed by repeated evacuation-refill with N2. The reaction mixture was heated in an oil bath at 100° C. overnight to afford a crude solution of the product that was used as the crude solution in the subsequent step. LCMS: [M+H]+=315.16.
Step 2: 3-chloro-6-fluoro-5-(4-((1S,4S)-5-methyl-2,5-diazabicyclo[2.2.1]heptan-2-yl)phenyl)pyridin-2-amine
A procedure similar to General Method A in Example 1, Step 2 using 5-bromo-3-chloro-6-fluoropyridin-2-amine (61 mg, 0.27 mmol, prepared according to Example 9), Cs2CO3 (264 mg, 0.811 mmol), PdCl2dppf (19.79 mg, 0.027 mmol), H2O (2 mL), (1S,4S)-2-methyl-5-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,5-diazabicyclo[2.2.1]heptane (85 mg, 0.270 mmol) in 1,4-dioxane (6 mL), DME (1.3 mL) afforded after workup and purification the product as a tan solid (113.0 mg, quantitative yield based on purity of 84%). LCMS: [M+H]+=333.50.
Step 3: 6-(2-amino-6-fluoro-5-(4-(5-methyl-2,5-diazabicyclo[2.2.1]heptan-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to General Method A (Example 1, Step 2) using 3-chloro-6-fluoro-5-(4-((1 S,4S)-5-methyl-2,5-diazabicyclo[2.2.1]heptan-2-yl)phenyl)pyridin-2-amine (0.113 g, 0.285 mmol), K3PO4 (0.181 g, 0.855 mmol), 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.086 g, 0.314 mmol), H2O (6 mL), MeCN (9 mL) and XPhos Pd G2 (0.022 g, 0.0285 mmol) afforded the title compound as a white solid (25 mg, 20%). 1H NMR (500 MHz, DMSO-d6): δ ppm 7.86 (br s, 1H), 7.83 (d, J=8.0 Hz, 1H), 7.49 (d, J=10.2 Hz, 1H), 7.39 (br d, J=8.0 Hz, 1H), 7.37 (s, 1H), 7.27 (br d, J=8.0 Hz, 2H), 6.54 (br d, J=8.7 Hz, 1H), 6.04 (s, 2H), 4.24 (s, 1H), 3.30-3.40 (m, 3H), 3.06-3.14 (m, 1H), 2.83-2.91 (m, 2H), 2.67-2.77 (m, 1H), 2.19 (s, 3H), 1.76-1.83 (m, 1H), 1.68-1.72 (m, 1H). LCMS: [M+H]+=444.32.
Step 1: 6-bromo-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Sodium azide (114 mg, 1.75 mmol, 2 eq.) was added to a solution of 5-bromo-6-fluoro-2,3-dihydro-1H-inden-1-one (200 mg, 0.873 mmol, 1 eq) in 0.4 mL of mixture of methane sulfonic acid and CH2Cl2 (1:1) in portionwise at 0° C. The resulting mixture was stirred for 8 h at RT. The reaction mixture was cooled to 0° C. in ice bath, neutralized with 5% aq. NaOH and aqueous layer was extracted with EtOAc (2×10 mL). The combined organic layers were washed with H2O and brine solution, dried over MgSO4 and filtered. The filtrate was concentrated under vacuum and purified by silica gel flash column chromatography using CH2Cl2-MeOH to obtain the product (150 mg, 70%). LCMS: [M+H]+=246.20 Step 2: 8-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroiso-quinolin-1(2H)-one
To 6-bromo-5-fluoro-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.205 mmol, 1eq), bis(pinacolato)diboron (58.2 mg, 0.229 mmol, 1.1 eq), K2CO3 (61.3 mg, 0.625 mmol, 3 eq) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (7.62 mg, 10.41 μmol, 0.05 eq) in 1,4-dioxane (2.5 mL) was added under Ar. The mixture was heated in a microwave for 2 h at 100° C. The LCMS showed less than 10% of starting material. The reaction mixture was used in the next steps without purification. LCMS: [M+H]+=292.0
Step 3: 3-chloro-6-fluoro-5-(4-morpholinophenyl)pyridin-2-amine
XPhos Pd G2 (20.39 mg, 0.026 mmol), 4-(morpholino)phenylboronic acid (248 mg, 1.198 mmol), 5-bromo-3-chloro-6-fluoropyridin-2-amine (270 mg, 2.0 mmol, preparation described in Example 9), and acetonitrile (3 mL) were added to a vial. K3PO4 (165 mg, 0.778 mmol) in H2O (2 mL) was added to the reaction mixture. The reaction was degassed with N2 and heated sealed in a microwave reactor at 100° C. for 4 h. The reaction mixture was worked up and purified in a manner similar to previous examples to afford the desired product (280 mg, 72%). LCMS: [M+H]+=308.24.
Step 4: 6-(2-amino-6-fluoro-5-(4-morpholinophenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A small vial was charged with XPhos Pd G2 (20.4 mg, 0.026 mmol) and 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (83 mg, 0.285 mmol) and in acetonitrile (3 mL). A solution of K3PO4 (165 mg, 0.778 mmol) in water (2 mL) was added to the reaction mixture. The reaction was degassed with N2 and heated in a sealed microwave reactor at 100° C. for 4 h. The mixture was then evaporated with Celite® and purified in a manner similar to previous examples to afford the title compound (19 mg, 16%). 1H NMR (500 MHz, DMSO-d6) δ 8.10 (br s, 1H), 7.60 (t, J=9.5 Hz, 2H), 7.45-7.38 (m, 3H), 6.98 (br d, J=8.8 Hz, 2H), 6.21-6.16 (m, 2H), 3.78-3.73 (m, 4H), 3.43-3.34 (m, 2H), 3.17-3.10 (m, 4H), 2.92 (br t, J=6.4 Hz, 2H), 2.64-2.62 (m, 1H), 2.46-2.38 (m, 1H), 2.37-2.35 (m, 1H); LCMS: [M+H]+=437.32.
To a slurry of 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.072 mmol, preparation described in Example 17) in DMF (2 mL) was added 2-fluoroethyl 4-methylbenzenesulfonate (23.52 mg, 0.108 mmol) and Et3N (0.100 ml, 0.719 mmol) at RT, and the mixture was heated in an oil bath at 100° C. for 12 h. The mixture was allowed to cool to RT, concentrated onto Celite, and purified on a reverse phase column eluting with water containing 0-35% acetonitrile. The material further purified by normal phase silica gel chromatography, eluting with DCM containing 0-2.5% MeOH and 0-0.25% NH4OH yielded product as a white powder (9 mg, 26%). 1H NMR (500 MHz, DMSO-d6) δ 7.8-8.0 (m, 2H), 7.6-7.7 (m, 1H), 7.4-7.5 (m, 2H), 7.41 (br d, J=8.1 Hz, 2H), 6.9-7.1 (m, 2H), 6.1-6.3 (m, 2H), 4.6-4.7 (m, 1H), 4.5-4.6 (m, 1H), 3.4-3.5 (m, 2H), 3.1-3.2 (m, 4H), 2.9-3.0 (m, 2H), 2.6-2.7 (m, 2H), 2.6-2.6 (m, 4H); LCMS: [M+H]+=464.47.
The procedure was similar to that of Example 20 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.072 mmol) and Et3N (0.100 ml, 0.719 mmol) in DMF (2 mL) at RT, with addition 2,2-difluoroethyl 4-methylbenzenesulfonate (25.5 mg, 0.108 mmol) instead of 2-fluoroethyl 4-methylbenzenesulfonate. The product (10.5 mg, 29%) was isolated as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.5-7.6 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 2H), 6.9-7.1 (m, 2H), 6.0-6.3 (m, 3H), 3.4-3.5 (m, 2H), 3.1-3.2 (m, 4H), 2.9-3.0 (m, 2H), 2.7-2.9 (m, 2H), 2.7-2.7 (m, 4H); LCMS: [M+H]+=482.52.
Step 1: tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenoxy)piperidine-1-carboxylate
To a stirred solution of 5-bromo-3-chloro-6-fluoropyridin-2-amine (100 mg, 0.444 mmol, 1.00 eq), tert-butyl 4-[4-(tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy]piperidine-1-carboxylate (192 mg, 0.476 mmol, 1.07 eq) in 1,4-Dioxane (5 ml), was added a solution of Cesium carbonate (434 mg, 1.331 mmol, 3.00 eq) in Water (1 ml) and the resulted reaction mixture was degassed under argon for 15 minutes. Then PddppfCl2 (48.7 mg, 0.067 mmol, 0.15 eq) was added and the reaction mixture was heated at 90° C. for overnight. The reaction mixture was dry loaded onto celite and purified by Flash [0-20% DCM/MeOH] to afford the product (187 mg, 100% yield) as a dark beige powder which will be used for the next step. [M+H]+ 422.35.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate
To a stirred solution of 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenoxy)piperidine-1-carboxylate (187 mg, 0.443 mmol, 1.00 eq), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (182 mg, 0.665 mmol, 1.50 eq) in 1,4-dioxane (5 ml), was added a solution of potassium phosphate tribasic (282 mg, 1.330 mmol, 3.00 eq) in Water (1 ml) and the resulted mixture was degassed under argon for 15 minutes. Then XPhos Pd G2 (34.9 mg, 0.044 mmol, 0.10 eq) was added and the reaction mixture was heated at 90° C. for 1 hour under microwave condition. The reaction mixture was then dry loaded onto celite and purified by Flash chromatography [0-10% DCM/MeOH] to afford the product (199 mg, 84% yield) as a dark beige powder which will be used for the next step. LCMS: [M+H]+=533.33.
Step 3: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate (199 mg, 1 eq) in CH2Cl2 (5 ml), was added TFA (2 ml, 69.79 eq). The resulted reaction mixture was stirred for 1 hour until completion of the reaction. The solvents were removed and the crude mixture was dissolved in MeOH and passed through a PoraPak Rxn CX (20cc-2g) cartridge in a catch & elute method. The cartridge was washed with MeOH (20 mL) and then the solution of product in MeOH was added onto the cartridge. The cartridge was rinsed with MeOH (3×20 mL) followed by 1× with a solution of 10 mL (NH3 in MeOH @ 7N) in 10 mL of MeOH to release the title compound (124.8 mg, 73.4% yield) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ=8.00-7.86 (m, 2H), 7.59 (br d, J=10.1 Hz, 1H), 7.50-7.40 (m, 4H), 6.98 (br d, J=8.7 Hz, 2H), 6.21 (s, 2H), 4.47-4.34 (m, 1H), 3.40 (br d, J=3.9 Hz, 2H), 3.01-2.89 (m, 4H), 2.57 (br s, 3H), 1.91 (br d, J=9.7 Hz, 2H), 1.45 (br d, J=9.5 Hz, 2H); LCMS: [M+H]+=433.33.
A sealed mixture of octahydropyrazino[2,1-c][1,4]oxazine (250 mg, 1.758 mmol), K3PO4 (1119 mg, 5.27 mmol) and 1,4-diiodobenzene (580 mg, 1.758 mmol) in DMF (10 mL) was degassed with a stream of N2. Later Cul (67.0 mg, 0.352 mmol) and (R)-(+)-1,1′-bi-2-naphthol (101 mg, 0.352 mmol) were added as solids. The degassing step was repeated. The mixture was then stirred sealed and heated at 40° C. for 2 d. The reaction mixture was cooled to RT, diluted with EtOAc and filtered through Celite®. The filtrate was concentrated under reduced pressure and purified by chromatography (50 g SiO2 Biotage@cartridge, using MeOH in CH2Cl2 eluting at 9% MeOH) to afford the product as a beige solid (310.0 mg, 79%). LCMS: [M+H]+=345.41.
Step 2: 3-chloro-6-fluoro-5-(4-(hexahydropyrazino[2,1-c][1,4]oxazin-8(1H)-yl)phenyl)pyridin-2-amine
A procedure similar to that described in Example 1, General Method A using 8-(4-iodophenyl)octahydropyrazino[2,1-c][1,4]oxazine (108 mg, 0.314 mmol), PdCl2dppf (22.95 mg, 0.031 mmol), Cs2CO3 (307 mg, 0.941 mmol), H2O (2 mL), DME (1.8 mL) and 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (94 mg, 0.345 mmol) in 1,4-dioxane (2.6 mL) and heating under microwave irradiation at 90° C. for 1 h afforded product as an brown solid (127.0 mg, 77% yield based on purity of 69%). LCMS: [M+H]+=363.22.
Step 3: 6-(2-amino-6-fluoro-5-(4-(hexahydropyrazino[2,1-c][1,4]oxazin-8(1H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to that described in Example 1, General Method A using 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.103 g, 0.377 mmol), K3PO4 (0.200 g, 0.942 mmol), 3-chloro-6-fluoro-5-(4-(hexahydropyrazino[2,1-c][1,4]oxazin-8(1H)-yl)phenyl)pyridin-2-amine (0.114 g, 0.314 mmol), and XPhos Pd G2 (0.025 g, 0.031 mmol) in H2O (9 mL) in CH3CN (12 mL), and afforded, after purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 9% MeOH) and preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in (H2O+0.05% TFA) eluting at 38% MeOH) the TFA salt of the title compound as a pale yellow solid (34.0 mg, 18%) 1H NMR (500 MHz, CD3OD) δ 8.03 (d, J=8.0 Hz, 1H), 7.59 (d, J=9.8 Hz, 1H), 7.47 (br d, J=8.1 Hz, 3H), 7.44 (s, 1H), 7.08 (brd, J=8.7 Hz, 2H), 4.14 (br d, J=9.2 Hz, 2H), 3.83-3.99 (m, 3H), 3.58-3.68 (m, 3H), 3.46-3.57 (m, 3H), 3.33-3.45 (m, 2H), 3.12-3.21 (m, 1H), 3.01-3.07 (m, 2H), 2.79-2.89 (m, 1H); LCMS: [M+H]+=474.31.
Step 1: (S)-1-(4-(4-iodophenyl)piperazin-1-yl)-2-methoxypropan-1-one
To a suspension of 1-(4-iodophenyl)piperazine hydrochloride (300 mg, 0.924 mmol) and TBTU (297 mg, 0.924 mmol) in anhydrous DMF (5 mL) was added N,N-diisopropylethylamine (0.483 mL, 2.77 mmol) followed by (S)-(−)-2-methoxypropionic acid (96 mg, 0.924 mmol) at RT. The reaction was stirred at RT overnight, then concentrated under reduced pressure, deposited on Biotage® samplet and purified by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 7% MeOH) to afford the desired product as a reddish gum (335 mg, 95% yield based on purity of 98%). LCMS: [M+H]+=375.26.
Step 2: (S)-1-(4-iodophenyl)-4-(2-methoxypropyl)piperazine
A dried vial was charged with (S)-1-(4-(4-iodophenyl)piperazin-1-yl)-2-methoxypropan-1-one (117 mg, 0.306 mmol) and filled with N2. Anhydrous THF (10 mL) was added followed by (CH3)2S·BH3 (2 M in THF, 0.46 mL, 0.92 mmol). After 5 min of stirring at RT the reaction was heated at 60° C. for 19.5 h. The reaction was then cooled to RT, carefully quenched with 2 M aq NaOH and concentrated under reduced pressure. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 2% MeOH) afforded the product as a white solid (75.0 mg, 53% yield based on purity of 78%). LCMS: [M+H]+=361.19.
Step 3: (S)-3-chloro-6-fluoro-5-(4-(4-(2-methoxypropyl)piperazin-1-yl)phenyl)pyridin-2-amine
A procedure similar to Example 1, General Method A using (S)-1-(4-iodophenyl)-4-(2-methoxypropyl)piperazine (0.075 g, 0.208 mmol), PdCl2dppf (0.013 g, 0.017 mmol), Cs2CO3 (0.170 g, 0.523 mmol), H2O (2 mL), DME (5 mL) and 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (1.571 mL, 0.209 mmol) in 1,4-dioxane (1.6 mL) afforded the crude product. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 15% MeOH) afforded the product as a brown solid (109.0 mg, quant yield based on purity of 66%). LCMS: [M+H]+=379.32.
Step 4: (S)-6-(2-amino-6-fluoro-5-(4-(4-(2-methoxypropyl)piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.067 g, 0.247 mmol), K3PO4 (0.121 g, 0.570 mmol), XPhos Pd G2 (0.015 g, 0.019 mmol), H2O (6 mL), (S)-3-chloro-6-fluoro-5-(4-(4-(2-methoxypropyl)piperazin-1-yl)phenyl)pyridin-2-amine (0.109 g, 0.190 mmol) and MeCN (9 mL) afforded the crude product. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 9% MeOH) followed preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in (H2O+0.05% TFA) eluting at 38% MeOH), afforded (S)-6-(2-amino-6-fluoro-5-(4-(4-(2-methoxypropyl)piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one·TFA as an off white solid (32.0 mg, 27% yield based on purity of 95%). 1H NMR (500 MHz, CD3OD) b 8.05 (d, J=8.0 Hz, 1H), 7.63 (d, J=9.9 Hz, 1H), 7.50 (br d, J=8.7 Hz, 3H), 7.47 (s, 1H), 7.10 (brd, J=8.8 Hz, 2H), 3.77-3.99 (m, 3H), 3.62-3.75 (m, 2H), 3.51-3.61 (m, 2H), 3.45 (s, 3H), 3.21-3.30 (m, 4H), 3.03-3.11 (m, 2H), 1.26 (d, J=6.0 Hz, 3H). Signals corresponding to two aliphatic protons are obscured by the solvent/water peak. LCMS: [M +H]+=490.29.
Step 1: (R)-1-(4-(4-iodophenyl)piperazin-1-yl)-2-methoxypropan-1-one
To a suspension of 1-(4-iodophenyl)piperazine hydrochloride (300 mg, 0.924 mmol) and TBTU (297 mg, 0.924 mmol) in anhydrous DMF (5 mL) was added N,N-diisopropylethylamine (0.483 mL, 2.77 mmol) followed by (R)-2-methoxypropanoic acid (96 mg, 0.924 mmol) at RT. The reaction was stirred at RT overnight, then concentrated under reduced pressure, deposited on Biotage® samplet and purified by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2) to afford the product as a reddish gum (392 mg, quant). LCMS: [M+H]+=375.03.
Step 2: (R)-1-(4-iodophenyl)-4-(2-methoxypropyl)piperazine
A vial was charged with (R)-1-(4-(4-iodophenyl)piperazin-1-yl)-2-methoxypropan-1-one (118 mg, 0.309 mmol) and filled with N2. Anhydrous THF (10 mL) was added, followed by (CH3)2S·BH3 (2M in THF, 0.46 mL, 0.93 mmol). The reaction mixture was stirred at RT for 5 min and then heated at 60° C. overnight. After quenching with 2 M aq NaOH at RT, the mixture was concentrated under reduced pressure. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 0-2% MeOH) afforded the product as a white solid (77.0 mg, 64% yield based on purity of 92%). LCMS: [M+H]+=361.04.
Step 3: (R)-3-chloro-6-fluoro-5-(4-(4-(2-methoxypropyl)piperazin-1-yl)phenyl)pyridin-2-amine
A procedure similar to Example 1, General Method A using (R)-1-(4-iodophenyl)-4-(2-methoxypropyl)piperazine (0.077 g, 0.197 mmol), PdCl2dppf (0.013 g, 0.017 mmol), Cs2CO3 (0.170 g, 0.523 mmol), H2O (2 mL), DME (2 mL) and crude 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (57 mg, 0.209 mmol) in 1,4-dioxane (1.74 mL) heating oil bath at 90° C. for 1.3 h afforded the crude product. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 9% MeOH) afforded the product as an orange-tan solid (46.0 mg, 43% yield based on purity of 70%). LCMS: [M+H]+=379.32.
Step 4: Synthesis of (R)-6-(2-amino-6-fluoro-5-(4-(4-(2-methoxypropyl)piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A, using 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.030 g, 0.110 mmol), K3PO4(0.054 g, 0.255 mmol), XPhos Pd G2 (6.69 mg, 8.50 μmol), H2O (6 mL), (R)-3-chloro-6-fluoro-5-(4-(4-(2-methoxypropyl)piperazin-1-yl)phenyl)pyridin-2-amine (0.046 g, 0.085 mmol) and MeCN (9 mL) and heating in an oil bath at 100° C. for 3 h afforded the crude product. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 9% MeOH) followed by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in (H2O+0.05% TFA) eluting at 40% MeOH) afforded the TFA salt of the title compound as a yellow-tan gum (37.0 mg, 70% yield based on purity of 97%). 1H NMR (500 MHz, CD3OD) δ 8.05 (d, J=8.0 Hz, 1H), 7.63 (d, J=9.8 Hz, 1H), 7.50 (br d, J=8.4 Hz, 3H), 7.47 (s, 1H), 7.10 (br d, J=8.7 Hz, 2H), 3.81-3.98 (m, 3H), 3.62-3.74 (m, 2H), 3.54-3.60 (m, 2H), 3.45 (s, 3H), 3.11-3.30 (m, 5H), 3.04-3.10 (m, 2H), 1.26 (d, J=6.0 Hz, 3H). A signal corresponding to an aliphatic proton is obscured by the solvent.
Step 1: 5′-chloro-2′-fluoro-5-morpholino-[2, 3′-bipyridin]-6′-amine
A procedure similar to Example 1, General Method A using 4-(6-bromo pyridin-3-yl)morpholine (76 mg, 0.313 mmol), PdCl2dppf (22.87 mg, 0.031 mmol), Cs2CO3 (305 mg, 0.938 mmol), H2O (2 mL), DME (1.2 mL), 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (102 mg, 0.375 mmol) in 1,4-dioxane (2.8 mL) and heating in an oil bath at 90° C. for 1.5 h afforded the crude product. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 6% MeOH) afforded the product as a light orange solid (53 mg, 50% yield based on purity of 91%). LCMS: [M+H]+=309.29.
Step 2: 6-(6′-amino-2′-fluoro-5-morpholino-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to General Method A, Example 1 using 5′-chloro-2′-fluoro-5-morpholino-[2,3′-bipyridin]-6′-amine (53 mg, 0.156 mmol), K3PO4 (99 mg, 0.469 mmol), 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (55.5 mg, 0.203 mmol), H2O (6 mL), MeCN (9 mL), XPhos Pd G2 (12.29 mg, 0.016 mmol); by heating in an oil bath at 100° C. for 3.4 h. Purification by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 5% MeOH) followed by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in H2O eluting at 66% MeOH) and a filtration through a Waters PoraPak CX afforded the title compound as a pale yellow solid (9 mg, 13% yield based on purity of 95%). 1H NMR (500 MHz, CD3OD) δ 8.28 (br s, 1H), 8.06 (d, J=7.8 Hz, 1H), 8.00 (br d, J=9.4 Hz, 1H), 7.75 (br s, 1H), 7.56-7.63 (m, 1H), 7.54 (br d, J=7.7 Hz, 1H), 7.49 (s, 1H), 3.86-3.91 (m, 4H), 3.55-3.60 (m, 2H), 3.26-3.30 (m, 4H), 3.04-3.11 (m, 2H); LCMS: [M+H]+=420.39.
Step 1: 5′-chloro-2′-fluoro-5-(4-methylpiperazin-1-yl)-[2, 3′-bipyridin]-6′-amine
A procedure similar to that described in Example 1, General Method A using 1-(6-bromopyridin-3-yl)-4-methylpiperazine (0.080 g, 0.312 mmol), PdCl2dppf (0.023 g, 0.031 mmol), Cs2CO3 (0.305 g, 0.938 mmol), H2O (2 mL), DME (1.2 mL) and 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (2.82 mL, 36.25 mg/mL, 0.375 mmol) in 1,4-dioxane (2.8 mL) and heating in a microwave reactor at 90° C. for 1 h provided after purification of the crude product by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) to give the product as a light orange solid (70 mg, 70% yield). LCMS: [M+H]+=322.23.
Step 2: Step 3: 6-(6′-amino-2′-fluoro-5-(4-methylpiperazin-1-yl)-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-1(2H)-one, acetic acid
Following a procedure similar to Example 1, General Method A, a degassed and sealed mixture of 5′-chloro-2′-fluoro-5-(4-methylpiperazin-1-yl)-[2,3′-bipyridin]-6′-amine (70 mg, 0.218 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (77 mg, 0.283 mmol), K3PO4 (139 mg, 0.653 mmol), H2O (6 mL), MeCN (9 mL) and XPhos Pd G2 (171 mg, 0.218 mmol) was heated in an oil bath at 100° C. for 4 h. The crude product was purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) to afford the title compound as an off white solid (35.0 mg, 33% yield based on purity of 99.7%). 1H NMR (500 MHz, DMSO-d6) δ 8.35 (d, J=2.7 Hz, 1H), 8.09 (d, J=10.0 Hz, 1H), 7.87-8.00 (m, 2H), 7.60 (br d, J=8.1 Hz, 1H), 7.35-7.46 (m, 3H), 6.36 (s, 2H), 3.39-3.43 (m, 2H), 3.22 (br d, J=4.5 Hz, 4H), 2.94-3.01 (m, 2H), 2.44-2.48 (m, 4H), 2.24 (s, 3H), 1.91 (s, 3H); LCMS: [M+H]+=433.33.
Step 1: dimethyl(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)silanol
A microwave vial was charged with dimethyl(4-chlorophenyl)silanol (250 mg, 1.339 mmol), B2pin2 (340 mg, 1.339 mmol), KOAc (394 mg, 4.02 mmol), XPhos Pd G2 (105 mg, 0.134 mmol). 1,4-Dioxane (10 mL) was added and the reaction was degassed with N2. The sealed vial was than heated in an oil bath at 90° C. for 18 h. The resulting product used as the crude solution (37.3 mg/mL) in the subsequent Suzuki-Miyaura cross coupling reaction.
Step 2: (4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenyl)dimethylsilanol
A procedure similar to Example 1, General Method A using of 5-bromo-3-chloro-6-fluoropyridin-2-amine (80 mg, 0.355 mmol), Cs2CO3 (347 mg, 1.065 mmol), PdCl2dppf (26.0 mg, 0.035 mmol), H2O (1.6 mL), dimethyl(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)silanol (118 mg, 37.3 mg/mL, 0.426 mmol) in 1,4-dioxane (3.2 mL) and heating at 90° C. for 1 h afforded, after purification by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) the product as a tan solid (60.0 mg, 41% yield based on purity of 72%). LCMS: [M+H]+=297.26.
Step 3: 6-(2-amino-6-fluoro-5-(4-(hydroxydimethylsilyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (71.8 mg, 0.263 mmol), K3PO4 (129 mg, 0.606 mmol), (4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenyl)dimethylsilanol (60 mg, 0.145 mmol, 72%) in H2O (6 mL), MeCN (12 mL) and XPhos Pd G2 (15.91 mg, 0.020 mmol) and heating in a microwave reactor at 100° C. for 2 h afforded, after purification by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) followed by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in H2O eluting at 60% MeOH), the title compound as an off white solid (13.0 mg, 21% yield based on purity of 97%). 1H NMR (500 MHz, CD3OD) δ 8.05 (d, J=7.8 Hz, 1H), 7.61-7.71 (m, 3H), 7.49-7.59 (m, 3H), 7.46 (s, 1H), 3.56 (br t, J=6.6 Hz, 2H), 3.07 (br t, J=6.5 Hz, 2H), 0.35-0.41 (m, 6H); LCMS: [M+H]+=408.28.
Step 1: 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine
To a vial containing 5-bromo-3-chloro-6-fluoropyridin-2-amine (425 mg, 1.89 mmol), bis(pinacolato)diboron (479 mg, 1.89 mmol), tris(dibenzylideneacetone)dipalladium (0) (173 mg, 0.189 mmol), tricyclohexylphosphine (52.9 mg, 0.189 mmol) and KOAc (370 mg, 3.77 mmol), was added 1,4-dioxane (15 mL). The reaction mixture was degassed with argon, the vial was sealed and then heated at 90° C. for 16 h. The reaction mixture was filtered through a Celite® bed, which was washed in with EtOAc (50 mL). The combined filtrate was concentrated under reduced pressure, prepared a standard stock solution of known concentration in DME and carried forward to subsequent step without further purification. LCMS: [M+H]+=273.34.
Step 2: 1-(4-iodophenyl)-4-(2-methoxyethyl)piperazine
A microwave vial containing a slurry of 1,4-diiodobenzene (500 mg, 1.516 mmol), 1-(2-methoxyethyl)piperazine (219 mg, 1.52 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (79 mg, 0.136 mmol), tris(dibenzylideneacetone)dipalladium (0) (41.6 mg, 0.045 mmol) and potassium tert-butoxide (510 mg, 4.55 mmol) in toluene (10 ml) was flushed with argon, was heated in an oil bath at 100° C. for 3 h. The reaction was partitioned between EtOAc (15 mL) and water (20 mL). The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-2% methanol and 0-0.2% ammonium hydroxide) afforded the product as a pale yellow solid (205 mg, 39%). LCMS: [M+H]+=347.25
Step 3: 3-chloro-6-fluoro-5-(4-(4-(2-methoxyethyl)piperazin-1-yl)phenyl)pyridin-2-amine
A microwave vial charged with [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (12.68 mg, 0.017 mmol), 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (55 mg, 0.202 mmol), 1-(4-iodophenyl)-4-(2-methoxyethyl)piperazine (50 mg, 0.144 mmol), DME (3.8 mL) and water (1 mL) was flushed with argon The vial was sealed and the reaction mixture was heated at 85° C. in a microwave reactor for 1.75 h. The reaction was partitioned between EtOAc and brine. The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-2% methanol and 0-0.2% NH4OH) followed by filtration through a Waters PoraPak CX column afforded the product as a beige solid (47 mg, 79% yield based on purity of 89%). LCMS: [M+H]+=365.37.
Step 4: 6-(2-amino-6-fluoro-5-(4-(4-(2-methoxyethyl)piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A microwave vial charged with XPhos Pd G2 (11.67 mg, 0.015 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (40.5 mg, 0.148 mmol, 3-chloro-6-fluoro-5-(4-(4-(2-methoxyethyl)piperazin-1-yl)phenyl)pyridin-2-amine (47 mg, 0.114 mmol), Cs2CO3 (112 mg, 0.342 mmol), 1,2-dimethoxyethane (5 ml) and water (1 ml) was flushed with argon. The vial was sealed and the reaction mixture was heated at 90° C. in a microwave reactor for 1 h. The reaction was partitioned between EtOAc and brine. The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-2% methanol and 0-0.2% NH4OH) followed by reverse phase chromatography (C18; 0-30% acetonitrile/water) afforded the title compound as a white solid (11 mg, 19% yield based on purity of 95%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 2H), 6.9-7.1 (m, 2H), 6.1-6.3 (m, 2H), 3.5-3.5 (m, 2H), 3.4-3.4 (m, 4H), 3.2-3.3 (m, 3H), 3.1-3.2 (m, 4H), 2.9-3.0 (m, 2H), 2.6-2.6 (m, 4H). LCMS: [M+H]+=476.56.
Step 1: 3-chloro-6-fluoro-5-(4-(piperidin-4-yl) phenyl) pyridin-2-amine
A procedure analogous to that of Example 29, Step 3 was used, by coupling 4-(4-bromophenyl)piperidine (150 mg, 0.625 mmol) and 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (221 mg, 0.812 mmol). After the standard workup and silica gel chromatography (eluting with CH2Cl2 containing 0-3.5% methanol and 0-0.35% NH4OH) followed by filtration through a Waters PoraPak CX column afforded the product as a beige solid (133 mg, 64% yield based on purity of 92%). LCMS: [M +H]+=306.38
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 29, Step 4 was used, coupling 3-chloro-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-2-amine (133 mg, 0.400 mmol) and 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (142 mg, 0.520 mmol). The reaction was partitioned between EtOAc and brine. The layers were separated and the aqueous layer was extracted with CHCl3/IPA 4:1 mix (2×3 mL). The combined organic extracts were dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-35% acetonitrile/water) followed by filtration through a Waters PoraPak CX column afforded the title compound as an off white solid (66 mg, 40% yield based on purity of 95%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.6-7.7 (m, 1H), 7.4-7.5 (m, 4H), 7.2-7.4 (m, 2H), 6.2-6.4 (m, 2H), 4.0-4.2 (m, 1H), 3.4-3.4 (m, 2H), 3.0-3.1 (m, 2H), 2.9-3.0 (m, 2H), 2.6-2.7 (m, 3H), 1.7-1.8 (m, 2H), 1.4-1.6 (m, 2H). LCMS: [M+H]+=417.52
Step 1: 5-bromo-6-fluoropyridin-2-amine:
To a solution of 6-fluoropyridin-2-amine (3 g, 26.8 mmol) in CH3CN (100 mL) under argon atmosphere was added NBS (4.7 g, 26.8 mmol) in CH3CN (80 mL) dropwise for 30 min at 0° C. and stirred for another 30 min at RT. The solvent was evaporated under reduced pressure and the resulting crude product was purified by column chromatography (silica gel, 100-200 mesh) using 10% EtOAc in pet ether as an eluent to afford the product (4.4 g, 88%) as a yellow solid. LCMS: [M+H]+=191.12.
Step 2: 5-bromo-3-chloro-6-fluoropyridin-2-amine:
To a solution of 5-bromo-6-fluoropyridin-2-amine (2 g, 10.5 mmol) in CH3CN (30 mL) was added NCS (1.4 g, 10.5 mmol) portionwise and the suspension was heated at reflux for 2 h at 90° C. under argon atmosphere. The reaction mixture was allowed to cool to RT, and was poured into ice-cold water (100 mL) to give a precipitate which was filtered to isolate the crude compound. Washing with n-pentane (100 mL) afforded the product (1.1 g, 48%) as an off-white solid. LCMS: [M+H]+=225.09.
Step 3: 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine
To a vial charged with B2pin2 (367 mg, 1.45 mmol), Pd2dba3 (132 mg, 0.145 mmol), PCy3 (40.6 mg, 0.145 mmol), 5-bromo-3-chloro-6-fluoropyridin-2-amine (326 mg, 1.446 mmol) and KOAc (284 mg, 2.89 mmol) was added anhydrous 1,4-dioxane (12 mL) was added. The suspension was degassed by evacuation and refilled with N2. The reaction mixture was then heated while sealed in an oil bath at 90-95° C. for 19 h. The resulting crude solution of product (36.2 mg/mL) was used in the subsequent step assuming quantitative conversion. LCMS: [M+H]+=273.27.
Step 4: 2-((4-bromophenyl)amino)ethan-1-ol:
To a solution of 1-bromo-4-iodobenzene (10 g, 35.5 mmol) in DMSO (100 mL) was added KOH (3.98 g, 70.9 mmol) and CuCl (0.7 g, 7.07 mmol) followed by ethanol amine (6.5 g, 106.4 mmol) at RT. The mixture was stirred at RT for 24 h, then quenched with ice water (100 mL) and extracted with EtOAc (2×100 mL). The combined organic layer was dried over Na2SO4 and concentrated under reduced pressure. The resulting residue was purified by column chromatography (using 100-200 silica gel) eluting with 30-40% EtOAc in pet ether to afford the product (5 g, 66%) as a pale yellow solid. LCMS: [M+H]+=216.23.
Step 5: 2-((4-bromophenyl)amino)ethyl methanesulfonate:
To a solution of 2-((4-bromophenyl)amino)ethan-1-ol (5 g, 23.2 mmol) in DCM (50 mL) was added triethylamine (9.8 mL, 69.7 mmol), followed by mesyl chloride (4.5 g, 58.2 mmol) at 0° C. to RT for 2 h. Then, the reaction mixture was poured into ice water (100 mL) and extracted with DCM (2×100 mL). The combined organic layers were dried over Na2SO4 and concentrated under reduced pressure to afford the crude product (5 g, quant) as a pale yellow solid. The crude compound was used without further purification. LCMS: [M+2H]+=295.99.
Step 6: 4-bromo-N-(2-(1-methylhydrazinyl)ethyl)aniline:
To a solution of 2-((4-bromophenyl)amino)ethyl methanesulfonate (5 g, 17.1 mmol) in ethanol (50 mL) was added DIPEA (14.3 mL, 85.3 mmol) followed by 85% aq. methyl hydrazine (10 mL, 2V) at RT. The mixture was heated to 80-85° C. for 2 h before cooling to RT. The solvent was concentrated under reduced pressure to give the crude compound which was poured into ice water (100 mL) and extracted with DCM (2×100 mL). The combined organic layers were dried over Na2SO4 and concentrated under reduced pressure to afford the crude product (3 g, 31%) as a pale brown solid. The crude compound was used without further purification. LCMS: [M+H]+: 244.22.
Step 7: 4-(4-bromophenyl)-1-methyl-1,4,5,6-tetrahydro-1,2,4-triazine:
To a solution of 4-bromo-N-(2-(1-methylhydrazinyl)ethyl)aniline (30.52% pure by LCMS) (5 g (1.5 g), 6.2 mmol) in AcOH (20 mL, 15V) was added trimethylorthoformate (20 mL, 15V) at 80° C. to 85° C. for 1 h before cooling to RT. The crude compound was poured into ice water (100 mL) basified with saturated NaHCO3 solution and extracted with DCM (2×50 mL). The combined organic layer was dried over Na2SO4 and concentrated under reduced pressure to give crude residue: which was purified by column chromatography (silica gel 100-200 mesh) using an eluent with 70-90% EtOAc in petroleum ether to afford the product (620 mg, 40%) as a pale yellow solid. LCMS: [M+H]+=254.25.
Step 8: 3-chloro-6-fluoro-5-(4-(1-methyl-5,6-dihydro-1,2,4-triazin-4(1H)-yl)phenyl)pyridin-2-amine
A method similar to General Method A using Pd(amphos)Cl2 (27.9 mg, 0.039 mmol), 4-(4-bromophenyl)-1-methyl-1,4,5,6-tetrahydro-1,2,4-triazine (100 mg, 0.393 mmol), K3PO4 (251 mg, 1.180 mmol), H2O (1 mL), 1,4-dioxane (7 mL) and 3-chloro-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (107 mg, 0.393 mmol, 36.2 mg/mL) in 1,4-dioxane solution (2.96 mL) and heating the degassed and sealed mixture under microwave irradiation at 90° C. for 1.5 h gave, after purification by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) the product as a brown solid (44% purity) that was used as is in the next step. LCMS: [M+H]+=320.12.
Step 9: 6-(2-amino-6-fluoro-5-(4-(1-methyl-5,6-dihydro-1,2,4-triazin-4(1H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
The procedure followed was similar to Example 1, General Method A using 3-chloro-6-fluoro-5-(4-(1-methyl-5,6-dihydro-1,2,4-triazin-4(1 H)-yl)phenyl)pyridin-2-amine (entire material from the previous step), K3PO4 (251 mg, 1.182 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (140 mg, 0.512 mmol), H2O (6 mL), MeCN (9 mL) and XPhos Pd G2 (31.0 mg, 0.039 mmol) and heating the sealed, degassed mixture in a microwave reactor at 100° C. for 1 h. The reaction mixture was then concentrated and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) followed, in sequence, by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in H2O), a filtration through a Waters PoraPak CX column and then another flash chromatography (50 g SiO2, using MeOH in CH2Cl2) to afford the title compound as a white solid (16.0 mg, 9% yield for 2 steps, based on purity of 99%). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.94 (br s, 1H), 7.91 (d, J=8.0 Hz, 1H), 7.63 (d, J=10.2 Hz, 1H), 7.48-7.53 (m, 3H), 7.45-7.48 (m, 1H), 7.42 (s, 1H), 7.18 (br d, J=8.7 Hz, 2H), 6.25 (s, 2H), 3.75 (br t, J=4.8 Hz, 2H), 3.37-3.45 (m, 2H), 2.90-2.99 (m, 4H), 2.65 (s, 3H); LCMS: [M+H]+=431.15.
Step 1: 3-chloro-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine
In a microwave vial with magnetic stir bar was placed 5-bromo-3-chloro-6-fluoropyridin-2-amine (260 mg, 1.15 mmol, preparation described in Example 31, Step 2), 4-(4-methylpiperazin-1-yl)phenylboronic acid, pinacol ester (383 mg, 1.27 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (84 mg, 0.115 mmol), Cs2CO3 (1127 mg, 3.46 mmol), DME (4 mL) and H2O (2 mL) (4:1 DME/H2O). The flask was sealed, then heated to 90° C. for 1 h in the microwave (high absorbance) when LCMS indicated complete conversion [01]. The reaction was loaded onto Celite® and purified by reverse phase chromatography (C18, MeCN—H2O) to give the product as a white solid (204 mg, 55%). 1H NMR (500 MHz, DMSO-d6) δ 7.80 (d, J=8.9 Hz, 1H), 7.35 (d, J=7.6 Hz, 2H), 6.96 (d, J=8.9 Hz, 2H), 6.66 (s, 2H), 3.18-3.14 (m, 4H), 2.46-2.42 (m, 4H), 2.22 (s, 3H); LCMS: [M+H]+=323.28.
Step 2: 5-(2-amino-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)isoindolin-1-one
In a microwave vial with magnetic stir bar was placed 3-bromo-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (55 mg, 0.151 mmol), 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoindolin-1-one (58.5 mg, 0.226 mmol), XPhos Pd G2 (5.92 mg, 7.53 μmol), Cs2CO3 (147 mg, 0.452 mmol), DME (4 mL) and H2O (2 mL) (4:1 DME/H2O). The flask was sealed, then heated to 90° C. for 1 h in a microwave (high absorbance), the reaction was then loaded onto Celite® and purified by reverse phase chromatography (C18, MeCN—H2O) to give the provide the title compound (12 mg, 18%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.61 (s, 1H), 7.78 (d, J=7.8 Hz, 1H), 7.74 (s, 1H), 7.67-7.61 (m, 2H), 7.45 (br d, J=8.1 Hz, 2H), 7.02 (br d, J=8.7 Hz, 2H), 6.22 (s, 2H), 4.46 (s, 2H), 3.24-3.18 (m, 4H), 2.55 (br s, 4H), 2.27 (s, 3H); LCMS: [M+H]+=418.29.
Step 1. methyl 4-bromo-2-(bromomethyl)benzoate (2):
To a stirred solution of methyl 4-bromo-2-methylbenzoate (10 g, 43.9 mmol) in CCl4 (100 mL), NBS (8.6 g, 48.3 mmol) and AIBN (0.730 g, 4.5 mmol) were added and the suspension was heated at reflux for 4 h at 80° C. under argon atmosphere before cooling to RT. The reaction mixture was filtered through the Celite® bed which was washed with diethyl ether (250 mL). The filtrate was concentrated under reduced pressure and dissolved in DCM (250 mL) and washed with cold water (3×50 mL). The combined organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure to afford the product (9.6 g, 71%) as a yellow oil. TLC: 20% EtOAc: Pet ether; Rf: 0.6
Step 2: 5-bromoisoindolin-1-one (3):
A solution of NH3 in methanol (100 mL, 7M) was added to the crude methyl 4-bromo-2-(bromomethyl)benzoate (6 g, 19.5 mmol) and heated to 90° C. for 16 h in a steel bomb before cooling to RT. The solid precipitate which was formed was filtered and washed with methanol (25 mL). The residue was dried to afford compound 3 (3.2 g, 78%) as a light brown solid. which was enough pure to be used in next step. LCMS: [M+2H]+=212.14.
Step 3: 5-bromo-2-(4-methoxybenzyl)-3,3-dimethylisoindolin-1-one (4):
To a stirred solution of tetra-n-butyl ammonium iodide (2.30 g, 6.2 mmol) in THF (190 mL), NaH (1.24 g, 51.6 mmol) was added at RT under argon atmosphere, followed by a solution 5-bromoisoindolin-1-one (6.4 g, 30.3 mmol) in DMF:THF (50:190 mL). After 75 min, 4-methoxybenzylbromide (4.6 mL, 31.9 mmol) was added and stirring was continued for 4 h. Then NaH (6.03 g, 251.3 mmol) was added and stirring was continued for 30 min before iodomethane (11.5 mL, 184.7 mmol) was added. The mixture was heated to 70° C. for 1 h before cooling to RT. The reaction mixture was then quenched with saturated aqueous NH4Cl solution (75 mL) and extracted with EtOAc (3×150 mL) and saturated brine solution (3×50 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford crude product which was purified using a silica flash cartridge (40 g column) using 8-15% EtOAc in pet ether as an eluent to afford the product (2.84 g, 27%) as yellow oil. LCMS: [M+2H]+=360.29.
Step 4. 5-bromo-3,3-dimethylisoindolin-1-one (5):
Ceric ammonium nitrate (13.13 g, 24.0 mmol) was added to a stirred solution of 5-bromo-2-(4-methoxybenzyl)-3,3-dimethylisoindolin-1-4 (2.84 g, 7.9 mmol) in acetonitrile (52 mL) and water (23 mL) at 0° C. and stirred for 1 h. The reaction mixture was extracted with EtOAc (3×100 mL) and saturated brine solution (3×50 mL). Combined organic layer was dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to give a crude product as yellow oil, which was purified using a silica flash cartridge (24 g column) using 10%-40% EtOAc in pet ether as an eluent to afford the product (1.56 g, 82%) as yellow solid. LCMS: [M+2H]+=240.21.
Step 5: 3,3-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoindolin-1-one
To a stirred degassed solution of 5-bromo-3,3-dimethylisoindolin-1-one (2.2 g, 9.2 mmol) in 1,4-dioxane (22 mL), KOAc (2.3 g, 23.4 mmol) and B2pin2 (3.12 g, 12.3 mmol) were added under argon atmosphere and degassed with argon for 20 minutes followed by the addition of Pd(dppf)Cl2.DCM (0.77 g, 0.94 mmol) and heated to 90° C. for 16 h before cooling to RT. The reaction mixture was filtered through Celite® bed which was washed with diethyl ether (250 mL). The filtrate was concentrated under reduced pressure to afford the crude compound as black gum, which was triturated with n-pentane (25 mL) and diethyl ether (10 mL) to afford the product (1.74 g, 66%) as black color solid. TLC: 20% EtOAc: DCM; Rf :0.3
Step 6. 5-(2-Amino-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,3-dimethylisoindolin-1-one
A procedure similar to that described in Example 33, Step 2 using 3-bromo-6-fluoro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (55 mg, 0.15 mmol) and 3,3-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoindolin-1-one (65 mg, 0.23 mmol) provided the title compound (27 mg, 38%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.64 (s, 1H), 7.74 (s, 1H), 7.66 (d, J=7.8 Hz, 1H), 7.60 (d, J=10.2 Hz, 1H), 7.57 (dd, J=7.8, 1.34 Hz, 1H), 7.41 (d, J=7.8 Hz, 2H), 6.97 (d, J=8.9 Hz, 2H), 6.13 (s, 2H), 3.13-3.18 (m, 4H), 2.42-2.47 (m, 4H), 2.22 (s, 3H), 1.48 (s, 6H); LCMS: [M+H]+=446.05.
Step 1: tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenoxy)piperidine-1-carboxylate
To a solution of 5-bromo-3-chloro-6-fluoropyridin-2-amine (100 mg, 0.444 mmol), tert-butyl 4-[4-(tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy]piperidine-1-carboxylate (192 mg, 0.476 mmol) in 1,4-dioxane (5 mL) was added a solution of Cs2CO3 (434 mg, 1.33 mmol) in water (1 mL) at RT. The reaction mixture was degassed under argon for 15 minutes before adding PddppfCl2 (48.7 mg, 0.067 mmol). Then the reaction mixture was heated at 90° C. for overnight. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-20%, DCM/MeOH) to afford the product (187 mg, 100%) as a dark beige powder which was used for the next step. LCMS: [M+H]+=422.35.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate
To a solution of tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenoxy)piperidine-1-carboxylate (187 mg, 0.443 mmol) and 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (182 mg, 0.665 mmol) in 1,4-dioxane (5 ml) was added a solution of K3PO4 (282 mg, 1.33 mmol) in water (1 mL) at RT. The reaction mixture was degassed under argon for 15 minutes before adding XPhos Pd G2 (34.9 mg, 0.044 mmol). Then the reaction mixture was heated at 90° C. for 1 hour under microwave. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-10%, DCM/MeOH) to afford the product (199 mg, 84%) as a dark beige powder which was used for the next step. LCMS: [M+H]+=533.33.
Step 3: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate (199 mg, 0.374 mmol) in CH2Cl2 (5 mL) was added TFA (2 mL, 26.1 mmol) at RT and the reaction mixture was stirred for 1 h. The solvents were removed and the crude mixture was dissolved in MeOH and passed through a PoraPak Rxn CX (20cc-2g) cartridge in a catch & elute method. The solvents were removed and the crude mixture was dissolved in MeOH and passed through a PoraPak Rxn CX (20cc-2g) cartridge in a catch & elute method. The cartridge was washed with MeOH (20 mL), then the solution of product in MeOH was added onto the cartridge and the cartridge was rinsed with MeOH (3×20 mL). The product was released using 20 mL of a solution of ammonia in MeOH (3N) to afford the title compound (124.8 mg, 73%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.86 (m, 2H), 7.59 (br d, J=10.1 Hz, 1H), 7.50-7.40 (m, 4H), 6.98 (br d, J=8.7 Hz, 2H), 6.21 (s, 2H), 4.47-4.34 (m, 1H), 3.40 (br d, J=3.9 Hz, 2H), 3.01-2.89 (m, 4H), 2.57 (br s, 3H), 1.91 (br d, J=9.7 Hz, 2H), 1.45 (br d, J=9.5 Hz, 2H); [M+H]+=433.33.
Step 4: 6-(5-(4-((1-acetylpiperidin-4-yl)oxy)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24.2 mg, 0.056 mmol) and N,N-diisopropylethylamine (0.019 ml, 0.112 mmol) in CH2Cl2 (3 mL) was added acetic anhydride (5.29 μl, 0.056 mmol) at RT. The reaction mixture was stirred for 1 h at RT. The reaction mixture was dry loaded and purified by flash chromatography (0-20%, MeOH/DC)] to afford the title compound (24.5 mg, 88%) as a white powder. 1H NMR (500 MHz, CD3OD) δ 8.03 (d, J=7.9 Hz, 1H), 7.61 (d, J=9.8 Hz, 1H), 7.49 (dd, J=8.1, 1.6 Hz, 1H), 7.48-7.42 (m, 3H), 7.02 (d, J=8.8 Hz, 2H), 4.72-4.63 (m, 1H), 3.87-3.72 (m, 2H), 3.61-3.47 (m, 4H), 3.05 (t, J=6.7 Hz, 2H), 2.13 (s, 3H), 2.07-2.01 (m, 1H), 1.96 (br t, J=4.0 Hz, 1H), 1.85-1.78 (m, 1H), 1.77-1.70 (m, 1H), NH and NH2 not observed; LCMS: [M+H]+=475.24.
To a solution of (4-((tetrahydro-2H-pyran-4-yl)oxy)phenyl)boronic acid (42 mg, 0.189 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (52 mg, 0.155 mmol) in 1,4-dioxane (5 mL) was added a solution of Cs2CO3 (151 mg, 0.464 mmol) in water (1 mL) at RT. The reaction mixture was degassed under argon for 15 min. Then PddppfCl2 (16.98 mg, 0.023 mmol) was added and the reaction mixture was heated at 90° C. for overnight. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-10%, DCM/MeOH) to afford the title compound (52.2 mg, 74%) as a beige powder. 1H NMR (500 MHz, CD3OD) δ 8.04 (d, J=8.1 Hz, 1H), 7.61 (d, J=9.8 Hz, 1H), 7.52-7.48 (m, 1H), 7.45 (br d, J=7.3 Hz, 3H), 7.01 (d, J=8.7 Hz, 2H), 4.65-4.56 (m, 1H), 4.02-3.91 (m, 2H), 3.60 (ddd, J=11.6, 8.7, 3.1 Hz, 2H), 3.57-3.52 (m, 2H), 3.35 (s, 2H), 3.10-3.01 (m, 2H), 2.10-2.00 (m, 2H), 1.80-1.68 (m, 2H), NH and NH2 not observed; LCMS: [M+H]+=434.31.
Step 1: 5-(2-amino-6-fluoropyridin-3-yl)-3,3-dimethylisoindolin-1-one
A procedure similar to that described in Example 1, General Method A, heating a sealed, degassed mixture of 3,3-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoindolin-1-one (254 mg, 0.884 mmol) (preparation described in Example 33, Step 5), K3PO4 (469 mg, 2.21 mmol), 3-chloro-6-fluoropyridin-2-amine (108 mg, 0.737 mmol), XPhos Pd G2 (53.7 mg, 0.068 mmol), H2O (6 mL) and MeCN (9 mL) in a microwave reactor at 100° C. for 2 h afforded, after purification by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) the product (214.0 mg, 97% yield based on purity of 91%). LCMS: [M+H]+=272.22.
Step 2: 5-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,3-dimethylisoindolin-1-one
To a stirred mixture of 5-(2-amino-6-fluoropyridin-3-yl)-3,3-dimethylisoindolin-1-one (214 mg, 0.718 mmol, 91%) in MeCN (10 mL), was added NBS (134 mg, 0.754 mmol) in one portion at 0° C. The reaction was allowed to warm slowly and stirred at RT overnight. The reaction mixture was concentrated and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) to afford the product as a light brown foam (298 mg, quant yield). LCMS: [M+H]+=350.14/352.17.
Step 3: 5-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,3-dimethylisoindolin-1-one
A vial was charged with 5-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,3-dimethylisoindolin-1-one (298 mg, 0.808 mmol), KOAc (159 mg, 1.62 mmol), PCy3 (22.67 mg, 0.081 mmol), B2pin2 (205 mg, 0.808 mmol) and Pd2dba3 (74.0 mg, 0.081 mmol). Anhydrous 1,4-dioxane (10 mL) was added and the suspension was degassed with a stream of N2. The reaction mixture was then heated sealed in an oil bath at 90° C. for 18 h and later at 110° C. for 2 h. Additional KOAc (159 mg, 1.62 mmol), Pd2dba3 (74.0 mg, 0.081 mmol), B2pin2 (205 mg, 0.808 mmol) and PCy3 (22.7 mg, 0.081 mmol) was then added; degassing was repeated and stirring was continued with heating at 110° C. for 2.5 h. The crude product (89% by LCMS) was used without further modification as the 1,4-dioxane mixture in the following step.
Step 4: 5-(2-amino-6-fluoro-5-(4-((1R,5S)-3-methyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,3-dimethylisoindolin-1-one
A procedure similar to that used in Example 1, General Method A using 5-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,3-dimethylisoindolin-1-one (124 mg, 0.278 mmol, 89% purity), in 1,4-dioxane (3.86 mL), PdCl2dppf (20.3 mg, 0.028 mmol), (1R,5S)-1-(4-bromophenyl)-3-methyl-3-azabicyclo[3.1.0]hexane (70 mg, 0.278 mmol), Cs2CO3 (271 mg, 0.833 mmol), H2O (5 mL) and DME (6 mL) and heating in a microwave reactor at 90° C. for 1.5 h afforded the title compound as an off-white solid (39.0 mg, 31% yield based on purity of 99%). 1H NMR (500 MHz, DMSO-d6) δ 8.65 (s, 1H), 7.75 (s, 1H), 7.62-7.68 (m, 2H), 7.58 (dd, J=7.8, 1.2 Hz, 1H), 7.48 (d, J=7.3 Hz, 2H), 7.19 (d, J=8.3 Hz, 2H), 6.25 (s, 2H), 3.17-3.30 (m, 2H), 3.00 (br d, J=8.2 Hz, 1H), 2.42 (br s, 1H), 2.28-2.36 (m, 3H), 1.76-1.92 (m, 1H), 1.49 (s, 6H), 1.36 (t, J=4.1 Hz, 1H), 0.78 (br dd, J=7.6, 3.6 Hz, 1H). LCMS: [M+H]+=443.11.
A procedure similar to that described in Example 1, General Method A using 5-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,3-dimethylisoindolin-1-one (123 mg, 0.275 mmol, preparation described in Example 33, Step 5) in 1,4-dioxane (3.83 mL), Pd (amphos)Cl2 (19.5 mg, 0.028 mmol), K3PO4 (175 mg, 0.826 mmol), 4-(4-bromophenyl)-1-methyl-1,4,5,6-tetrahydro-1,2,4-triazine (70 mg, 0.275 mmol) (preparation described in Example 31, Step 8), H2O (1.4 mL) and additional 1,4-dioxane (6 mL) afforded the TFA salt of the title compound as a light beige sticky solid (42.0 mg, 27%). 1H NMR (500 MHz, CD3OD) δ 8.64 (s, 1H), 7.86 (d, J=7.8 Hz, 1H), 7.68-7.76 (m, 4H), 7.63 (dd, J=7.8, 1.5 Hz, 1H), 7.49-7.54 (m, 2H), 4.05-4.12 (m, 2H), 3.40-3.44 (m, 2H), 2.90 (s, 3H), 1.58-1.63 (m, 6H); LCMS: [M+H]+=445.30.
Step 1: (R)-8-(4-iodophenyl)octahydropyrazino[2, 1-c][1,4]oxazine
The product was prepared by a method similar to Example 23, Step 1 using (R)-octahydro-pyrazino[2,1-c][1,4]oxazine ·2HCl (381 mg, 1.771 mmol), K3PO4 (1896 mg, 8.93 mmol), DMF (20 mL), 1,4-diiodobenzene (508 mg, 1.54 mmol), Cul (64.5 mg, 0.339 mmol) and (R)-(+)-1,1′-bi-2-naphthol (97 mg, 0.339 mmol) to afford the product as a light tan solid (279 mg, 53%). LCMS: [M+H]+=345.17.
Step 2: (R)-6-(2-amino-6-fluoro-5-(4-(hexahydropyrazino[2,1-c][1,4]oxazin-8(1H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A by heating a sealed and degassed mixture of (R)-8-(4-iodophenyl)octahydropyrazino[2,1-c][1,4]oxazine (80 mg, 0.232 mmol), PdCl2dppf (20.3 mg, 0.028 mmol), Cs2CO3 (227 mg, 0.697 mmol), H2O (3 mL), and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (111 mg, 0.232 mmol, 18.5 mg/mL, 80% purity) in 1,4-dioxane (6.02 mL) in a microwave reactor at 90° C. afforded, after purification by flash chromatography (25 g SiO2, eluting with MeOH in CH2Cl), the title compound as a beige solid (60.0 mg, 52% yield based on purity of 95%). 1H NMR (500 MHz, DMSO-d6) δ 7.88-7.97 (m, 1H), 7.59 (d, J=10.3 Hz, 1H), 7.44-7.49 (m, 2H), 7.41 (d, J=7.8 Hz, 2H), 6.98 (d, J=9.1 Hz, 2H), 6.18 (s, 2H), 3.77 (td, J=10.6, 2.6 Hz, 2H), 3.67 (br d, J=11.6 Hz, 1H), 3.50-3.59 (m, 2H), 3.35-3.45 (m, 3H), 3.13-3.21 (m, 1H), 2.96 (t, J=6.5 Hz, 2H), 2.73-2.86 (m, 2H), 2.69 (br d, J=11.7 Hz, 1H), 2.17-2.34 (m, 4H); LCMS: [M+H]+=474.41.
Step 1: (S)-8-(4-iodophenyl)octahydropyrazino[2,1-c][1,4]oxazine
A procedure similar to that described in Example 23, Step 1 using (S)-octahydropyrazino[2,1-c][1,4]oxazine·2HCl (376 mg, 1.75 mmol), K3PO4 (1871 mg, 8.82 mmol) 1,4-diiodobenzene (501 mg, 1.52 mmol), Cul (63.7 mg, 0.334 mmol) and (R)-(+)-1,1′-bi-2-naphthol (96 mg, 0.334 mmol) in DMF (20 mL) afforded the product as a rust orange solid (128 mg, 22% yield based on purity of 89%). LCMS: [M+H]+=345.17.
Step 2: (S)-6-(2-amino-6-fluoro-5-(4-(hexahydropyrazino[2,1-c][1,4]oxazin-8(1H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to that described in Example 1, General Method A, heating a mixture of (S)-8-(4-iodophenyl)octahydropyrazino[2,1-c][1,4]oxazine (80 mg, 0.233 mmol), PdCl2dppf (20.3 mg, 0.028 mmol), Cs2CO3 (227 mg, 0.698 mmol), H2O (3 mL) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (89 mg, 18.5 mg/mL, 0.233 mmol, 80%) in 1,4-dioxane (6.03 mL) afforded, after the mixture was concentrated under reduced pressure and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) followed by a filtration through a Waters PoraPak CX column the title compound as a beige solid (24 mg, 22% yield based on purity of 99%). 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.59 (d, J=10.2 Hz, 1H), 7.44-7.49 (m, 2H), 7.41 (br d, J=8.0 Hz, 2H), 6.98 (d, J=8.9 Hz, 2H), 6.18 (s, 2H), 3.71-3.82 (m, 2H), 3.67 (br d, J=11.6 Hz, 1H), 3.50-3.59 (m, 2H), 3.35-3.46 (m, 2H), 3.10-3.25 (m, 1H), 2.96 (br t, J=6.5 Hz, 2H), 2.72-2.86 (m, 2H), 2.65-2.71 (m, 1H), 2.17-2.34 (m, 4H); LCMS: [M+H]+=474.11.
A procedure analogous to Example 8 Step 4 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.30 mmol) and 3-fluoro-4-(4-methylpiperazino)phenylboronic acid pinacol ester (100 mg, 0.31 mmol) provided the title compound (26 mg, 18%) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=8.0 Hz, 1H), 7.65 (d, J=10.2 Hz, 1H), 7.47 (dd, J=8.0, 1.5 Hz, 1H), 7.45 (s, 1H), 7.35 (br d, J=14.6 Hz, 1H), 7.31 (br d, J=8.4 Hz, 1H), 7.05 (t, J=9.0 Hz, 1H), 6.30 (s, 2H), 3.40 (td, J=6.5, 2.7 Hz, 2H), 3.03 (br t, J=4.3 Hz, 4H), 2.95 (t, J=6.5 Hz, 2H), 2.47 (br s, 4H), 2.22 (s, 3H); LCMS: [M+H]+=450.26.
A procedure analogous to Example 8, Step 4 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.30 mmol) and (4-(4-ethylpiperazin-1-yl)phenyl)boronic acid pinacol ester (99 mg, 0.31 mmol) provided the title compound (25 mg, 18%) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.92 (d, J=8.0 Hz, 1H), 7.60 (d, J=10.3 Hz, 1H), 7.48 (dd, J=8.0, 1.5 Hz, 1H), 7.46 (s, 1 H), 7.41 (d, J=7.8 Hz, 2H), 6.98 (d, J=8.9 Hz, 2H), 6.18 (s, 2H), 3.42 (td, J=6.5, 2.6 Hz, 2H), 3.14-3.20 (m, 4H), 2.97 (t, J=6.5 Hz, 2H), 2.51 (br dd, J=3.6, 1.6 Hz, 4H), 2.38 (q, J =7.2 Hz, 2H), 1.05 (t, J=7.2 Hz, 3H); LCMS: [M+1]+=446.27.
In a microwave vial with magnetic stir bar was placed 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.297 mmol), Cs2CO3 (291 mg, 0.892 mmol), DME (4 mL) and water (2 mL). The flask was sealed, then heated to 90° C. for 1 h in the microwave. The reaction was loaded onto Celite® and purified by reverse phase chromatography (C18, MeCN—H2O) followed by silica gel chromatography (SiO2, 100% DCM to 95-5-1 DCM/MeOH/NH4OH) to give the title compound (19 mg, 14%) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ 7.86 (br s, 1H), 7.83 (d, J=8.0 Hz, 1H), 7.51 (d, J=10.2 Hz, 1H), 7.38-7.42 (m, 1H), 7.37 (s, 1H), 7.33 (d, J=7.8 Hz, 2H), 6.89 (d, J=8.9 Hz, 2H), 6.10 (s, 2H), 3.34 (td, J=6.5, 2.7 Hz, 2H), 3.05-3.10 (m, 4H), 2.88 (t, J=6.5 Hz, 2H), 2.56-2.64 (m, 1H), 2.48-2.53 (m, 4H), 0.94 (d, J=6.5 Hz, 6H); LCMS: [M+1]+=460.27.
Step 1: 4-(4-bromophenyl)-1-(2, 2-difluoroethyl)piperidine
To a slurry of 4-(4-bromophenyl)piperidine (400 mg, 1.67 mmol) in DMF (5 mL) at RT, was added 2,2-difluoroethyl trifluoromethanesulfonate (0.444 mL, 3.33 mmol), followed by Et3N (2.32 mL, 16.7 mmol) and the reaction mixture was stirred at RT for 10 min. The reaction mixture was concentrated to dryness and the residue was partitioned between CH2Cl2 and water. The layers were separated and the aqueous layer was extracted with CH2Cl2 (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-0.5% MeOH and 0-0.05% NH4OH) afforded the product as a pale yellow oil (384 mg, 76%). LCMS: [M+H]+=304.37.
Step 2: 5-bromo-6-fluoro-3-iodopyridin-2-amine:
To a solution of 5-bromo-6-fluoropyridin-2-amine (40 g, 210.5 mmol) in acetic acid (280 mL), was added N-iodo succinimide (47.4 g, 210.5 mmol) and TFA (2.6 mL) and the mixture was stirred at RT for 2.5 h, then poured into ice water and neutralized with aqueous ammonia. The resulting precipitate was filtered, washed with ice water, and dried over under vacuum to afford the product (60 g, 90%) as a yellow solid. LCMS: [M+H]+=316.8.
Step 3: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one:
To a degassed suspension of 5-bromo-6-fluoro-3-iodopyridin-2-amine (40 g, 126.6 mmol), 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (38.15 g, 139.3 mmol) and K2CO3 (34.95 g, 253.2 mmol) in a mixture of DMF (400 mL) and H2O (80 mL) was added Pd(PPh3)2Cl2, (8.87 g, 12.6 mmol). The reaction mixture was heated to 80° C. for 16 h before cooling to RT. The reaction mixture was poured into ice water and extracted with EtOAc (2×500 mL), washed with water (500 mL) and brine (500 mL), dried over Na2SO4 and concentrated under reduced pressure to give crude residue which was purified by column chromatography (silica gel, 100-200 mesh) using eluent 60-80% EtOAc in petroleum ether to afford the product (23 g, 54%) as a brown solid. LCMS: [M+H]+=336.04
Step 4: 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one:
To a degassed suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (10 g, 29.8 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (22.7 g, 89.5 mmol), and KOAc (10.23 g, 104.4 mmol) in toluene (200 mL) was added a Pd2(dba)3 (1.64 g, 1.8 mmol) and X-PHOS (1.42 g, 3 mmol). The reaction mixture was heated to 110° C. for 3 h under argon atmosphere before cooling to RT. The reaction mixture was filtered through a Celite® bed, which was washed with 20% MeOH in DCM (500 mL). Then, the filtrate was concentrated under reduced pressure to give residue; which was purified by washed with EtOAc (100 mL) to afford the product (9 g, 79%) as a pale brown solid. LCMS: [M+H]+=384.27
Step 5: 6-(2-amino-5-(4-(1-(2, 2-difluoroethyl)piperidin-4-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A microwave vial charged with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (70.9 mg, 0.148 mmol), 4-(4-bromophenyl)-1-(2,2-difluoroethyl)piperidine (30 mg, 0.099 mmol, prepared in Step 1), Cs2CO3 (96 mg, 0.296 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (10.82 mg, 0.015 mmol), 1,2-dimethoxyethane (3.25 mL) and H2O (2.5 mL) was flushed with Argon. The vial was sealed and heated in a microwave reactor at 90° C. for 1 h. The reaction was partitioned between EtOAc and brine. The layers were separated and the aqueous layer was extracted with EtOAc (x 2). The combined organic extracts were dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-35% acetonitrile/water) followed by filtration through a Waters PoraPak CX column afforded the title compound as a beige solid (15 mg, 30% yield based on purity of 95%). 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.87 (m, 2H), 7.68-7.60 (m, 1H), 7.55-7.42 (m, 4H), 7.35-7.26 (m, 2H), 6.34-6.27 (m, 2H), 6.26-5.99 (m, 1H), 3.46-3.39 (m, 2H), 3.05-2.99 (m, 2H), 2.98-2.92 (m, 2H), 2.81-2.71 (m, 2H), 2.58-2.55 (m, 1H), 2.32-2.24 (m, 2H), 1.80-1.73 (m, 2H), 1.72-1.64 (m, 2H). LCMS: [M+H]+=481.52
To a solution of 1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)piperidine (61.7 mg, 0.195 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (54.5 mg, 0.162 mmol) in 1,4-Dioxane (3 ml) was added a solution of Cs2CO3 (158 mg, 0.486 mmol) in water (1 mL) at RT and the reaction mixture was degassed under argon for 15 minutes. Then PddppfCl2 (17.79 mg, 0.024 mmol) was added and the reaction mixture was heated at 90° C. for 6 hours. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-20%, DCM/MeOH) to afford the title compound (66.6 mg, 87%) as a light brown powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.46 (br d, J=9.3 Hz, 4H), 7.00 (d, J=8.8 Hz, 2H), 6.22 (s, 2H), 4.48-4.39 (m, 1H), 3.43-3.38 (m, 2H), 2.95 (s, 2H), 2.80-2.68 (m, 2H), 2.43-2.17 (m, 5H), 2.02-1.93 (m, 2H), 1.70 (br s, 2H); LCMS: [M+H]+=447.33.
To a solution of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (63.5 mg, 0.189 mmol) and N1,N1,N2-trimethyl-N2-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)ethane-1,2-diamine (63.2 mg, 0.208 mmol) in 1,4-dioxane (3 mL) was added a solution of Cs2CO3 (185 mg, 0.567 mmol) in water (1 mL) at RT and the reaction mixture was degassed under argon for 15 minutes. Then PddppfCl2 (20.73 mg, 0.028 mmol) was added and the reaction mixture was heated at 90° C. for 6 h. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-20%, DCM/MeOH) to afford the title compound (70.5 mg, 82%) as a light brown powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.56 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 2H), 7.36 (br d, J=8.1 Hz, 2H), 6.73 (d, J=8.9 Hz, 2H), 6.12 (s, 2H), 3.46 (br t, J=7.1 Hz, 2H), 3.40 (br d, J=2.4 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.92 (s, 3H), 2.48-2.42 (m, 2H), 2.25 (s, 6H); LCMS: [M+H]+=434.31.
Step 1: (R)-1-(4-chlorophenyl)-2-methylpyrrolidine
A 30 mL vial was charged with (R)-2-methylpyrrolidine hydrochloride (0.30 g, 2.5 mmol), 1-chloro-4-iodobenzene (0.74 g, 3.1 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.068 g, 0.074 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.13 g, 0.22 mmol) and Cs2CO3 (4.0 g, 12 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (8 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated conventionally at 100° C. for 18 h. The reaction mixture was cooled to RT and partitioned between EtOAc and water. The layers were separated and the aqueous layer was extracted with additional EtOAc. The combined organic extracts were dried and concentrated onto Celite®. Flash chromatography (1-10% EtOAc/hexanes) afforded the product (0.18 g, 37%). LCMS: [M+H]+=196.1.
Step 2: (R)-2-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A 30 mL vial was charged with (R)-1-(4-chlorophenyl)-2-methylpyrrolidine (0.18 g, 0.91 mmol), bis(pinacolato)diboron (0.35 g, 1.4 mmol), KOAc (0.18 g, 1.8 mmol) and XPhos Pd G2 (0.054 g, 0.068 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-dioxane (8 mL) was added and the vial was evacuated and backfilled an additional time. The reaction mixture was then heated to 110° C. in an aluminum block for 18 h. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0-8% EtOAc/hexanes) to afford the product (14 mg, 52%). LCMS: [M+H]+=288.1.
Step 3: (R)-3-chloro-6-fluoro-5-(4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-2-amine
A 30 mL was charged with 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.050 g, 0.22 mmol), (R)-2-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.070 g, 0.24 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium (II) DCM complex (0.018 g, 0.022 mmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and 2 M aqueous Na2CO3 (0.33 mL, 0.66 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 90° C. for 18 h in an aluminum block. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0-10% EtOAc/hexanes) to afford the product (23 mg, 34%). LCMS: [M+H]+=306.3.
Step 4: (R)-6-(2-amino-6-fluoro-5-(4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.024 g, 0.086 mmol), (R)-3-chloro-6-fluoro-5-(4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-2-amine (0.023 g, 0.075 mmol) and XPhos Pd G2 (0.0059 mg, 0.0075 mmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (1.5 mL) and aqueous K3PO4 (0.17 mL of a 1.3 M solution, 0.23 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 100° C. for 18 h in an aluminum block. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0.5-10% DCM/MeOH+1% NH4OH). The product containing fractions were concentrated and further purified by reverse phase chromatography (Biotage SNAP C18; 5-60% MeCN/water+0.1% Formic Acid). Isolation of the title compound by a catch and release procedure using Biotage SCX2 silica gel afforded the title compound (8 mg, 23%). 1H NMR (500 MHz DMSO-d6) δ 7.8-7.9 (m, 2H), 7.48 (d, J=10.1 Hz, 1H), 7.4-7.4 (m, 2H), 7.28 (br d, J=7.9 Hz, 2H), 6.52 (d, J=8.8 Hz, 2H), 6.02 (br s, 1H), 3.81 (quin, J=5.7 Hz, 1H), 3.3-3.4 (m, 3H), 3.0-3.1 (m, 1H), 2.88 (br t, J=6.5 Hz, 2H), 1.8-2.0 (m, 3H), 1.6-1.6 (m, 1H), 1.04 (d, J=6.1 Hz, 3H,); LCMS: [M+H]+=417.5.
Step 1: (R)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-2-amine
A 30 mL vial was charged with (R)-2-methylpyrrolidine hydrochloride (0.300 g, 2.467 mmol), 4-bromo-1-chloro-2-fluorobenzene (0.359 mL, 3.08 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.068 g, 0.074 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.128 g, 0.222 mmol) and Cs2CO3 (4.02 g, 12.3 mmol). The vial was sealed with a cap and septum and then the reaction vessel was evacuated and backfilled with nitrogen. Toluene (8 mL) was added, the reaction vessel was evacuated and backfilled with nitrogen an additional time, and was heated at 100° C. for 18 h. The reaction was concentrated onto Celite® and purified by flash chromatography (1-10% EtOAc/hexanes) to afford the product (268 mg, 51%). LCMS: [M+H]+=214.02.
Step 2: (R)-1-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-methylpyrrolidine
A 30 ml vial was charged with (R)-1-(4-chloro-3-fluorophenyl)-2-methylpyrrolidine (0.268 g, 1.25 mmol), bis(pinacolato)diboron (0.478 g, 1.88 mmol), KOAc (0.246 g, 2.51 mmol) and XPhos Pd G2 (0.074 g, 0.094 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (8 mL) was added and the vial was evacuated and backfilled an additional time. The reaction mixture was then heated to 110° C. in an aluminum block for 18 h. The reaction mixture was concentrated onto Celite® and purified by flash (0-8% EtOAc/hexanes) to afford the product (244 mg, 64%). LCMS: [M+H]+=306.06.
Step 3: (R)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-2-amine
In a 30 mL vial with magnetic stir bar was charged 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.055 g, 0.244 mmol), (R)-1-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-methylpyrrolidine (0.082 g, 0.268 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.020 g, 0.024 mmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and aqueous Na2CO3 (0.366 mL of a 2 M solution, 0.732 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 90° C. for 18 h in an aluminum block. The reaction mixture was concentrated onto Celite® and purified by flash (0-10% EtOAc/hexanes) to afford the product (244 mg, 64%). LCMS: [M+H]+=306.06.
Step 3: (R)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-2-amine
In a 30 mL vial with magnetic stir bar was charged 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.055 g, 0.244 mmol), (R)-1-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-methylpyrrolidine (0.082 g, 0.268 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.020 g, 0.024 mmol) The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and aqueous Na2CO3 (2 M) (0.366 mL, 0.732 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 90° C. for 18 h in an aluminum block. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0-10% EtOAc/hexanes) to afford the product (34 mg, 43%). LCMS: [M+H]+=323.88.
Step 4: (R)-6-(2-amino-6-fluoro-5-(2-fluoro-4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.033 g, 0.121 mmol), (R)-3-chloro-6-fluoro-5-(2-fluoro-4-(2-methylpyrrolidin-1-yl)phenyl)pyridin-2-amine (0.034 g, 0.105 mmol) and XPhos Pd G2 (8.26 mg, 10.50 μmol) and aqueous K3PO4 (0.242 mL of a 1.3 M solution, 0.315 mmol) afforded the title compound (3 mg, 6%) as a pale yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.3-7.4 (m, 3H), 7.13 (t, J=8.8 Hz, 1H), 6.35 (dd, J=8.6, 2.3 Hz, 1H), 6.30 (dd, J=13.9, 2.2 Hz 1H), 6.13 (br d, J=2.4 Hz, 2H), 3.82 (quin, J=6.0 Hz, 1H), 3.3-3.4 (m, 4H), 3.0-3.1 (m, 1H), 2.87 (t, J=6.5 Hz, 2H), 1.9-2.0 (m, 4H), 1.6-1.6 (m, 1H), 1.04 (d, J=6.1 Hz, 3H); LCMS: [M+H]+=435.4.
Step 1: tert-butyl (R)-3-isopropyl-4-methylpiperazine-1-carboxylate
To a solution of (R)-1-Boc-3-isopropyl-piperazine (1.0 g, 4.4 mmol) in 1:1 MeOH:THF (20 mL) was added an aqueous formaldehyde solution (37%, 0.5 mL, 6.6 mmol) followed by NaBH(OAc)3 (1.4 g, 6.6 mmol). The reaction was stirred at RT for 18 h. The volatiles were removed in vaccuo and the residue was partitioned between aqueous KOH (1N) and DCM. The layers were separated and the aqueous layer was extracted with additional DCM (x2). The combined organic extracts were dried over MgSO4 and concentrated to dryness to afford the product (1.06 g, quantitative yield). LCMS: [M+H]+=243.3.
Step 2: (R)-2-isopropyl-1-methylpiperazine
Trifluoroacetic acid (2.0 mL, 26 mmol) was added to a solution of tert-butyl (R)-3-isopropyl-4-methylpiperazine-1-carboxylate (0.50 g, 2.1 mmol) in DCM (10 mL) at RT. The reaction mixture was allowed to stir at RT for 18 h. The volatiles were removed under a stream of compressed air and the residue was dried under reduced pressure to afford the trifluoroacetic acid salt of the product (0.91 g, quantitative yield). LCMS: [M+H]+=143.4.
Step 3: (R)-4-(4-chlorophenyl)-2-isopropyl-1-methylpiperazine
A 30 mL vial was charged with the trifluoroacetic acid salt of (R)-2-isopropyl-1-methylpiperazine (0.50 g, 1.4 mmol), 1-chloro-4-iodobenzene (0.40 g, 1.7 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.037 g, 0.041 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.070 g, 0.12 mmol) and Cs2CO3 (2.6 g, 8.1 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (6 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated conventionally at 100° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by flash chromatography (0.5-9.5% DCM/MeOH+0.5% NH4OH) to afford the product (0.18 g, 51%). LCMS: [M+H]+=253.3.
Step 4: (R)-2-isopropyl-1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine
A 30 mL vial was charged with (R)-4-(4-chlorophenyl)-2-isopropyl-1-methylpiperazine (0.18 g, 0.69 mmol), bis(pinacolato)diboron (0.26 g, 1.0 mmol), KOAc (0.14 g, 1.4 mmol) and XPhos Pd G2 (0.041 g, 0.052 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (6 mL) was added and the vial was evacuated and backfilled an additional time. The reaction mixture was then heated to 110° C. in an aluminum block for 18 h. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0.5-7.5% MeOH/DCM+0.5% NH4OH) to afford the product (0.20 g, 83%). LCMS: [M+H]+=345.4.
Step 5: (R)-3-chloro-6-fluoro-5-(4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-2-amine
A 30 mL was charged with 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.040 g, 0.18 mmol), (R)-2-isopropyl-1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (0.067 g, 0.20 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium (II) DCM complex (0.014 g, 0.018 mmol) . The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and 2 M aqueous Na2CO3 (0.27 mL, 0.53 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 90° C. for 18 h in an aluminum block. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0.5-9.5% MeOH/DCM+0.5% NH4OH) to afford the product (16 mg, 25%). LCMS: [M+H]+=363.2.
Step 6: (R)-6-(2-amino-6-fluoro-5-(4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.014 g, 0.051 mmol), (R)-3-chloro-6-fluoro-5-(4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (0.016 g, 0.044 mmol), XPhos Pd G2 (0.0035 mg, 0.0044 mmol) and aqueous K3PO4 (0.10 mL of a 1.3 M solution, 0.13 mmol) afforded the title compound (3 mg, 13%). 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.51 (d, J=10.1 Hz, 1H), 7.4-7.4 (m, 2H), 7.33 (br d, J=7.8 Hz, 2H), 6.90 (d, J=8.9 Hz, 2H), 6.10 (br s, 2H), 3.50 (br d, J=11.7 Hz, 1H), 3.41 (br d, J=11.6 Hz, 1H), 3.3-3.4 (m, 3H), 2.88 (br t, J=6.4 Hz, 3H), 2.8-2.8 (m, 1H), 2.65 (dt, J=11.7, 2.8 Hz, 2H), 2.2-2.3 (m, 2H), 2.13 (s, 3H), 2.05 (dt, J=6.9, 4.2 Hz, 1H), 1.87 (td, J=10.5, 3.4 Hz, 1H), 0.92 (d, J=7.0 Hz, 3H), 0.81 (d, J=7.0 Hz, 3H); LCMS: [M+H]+=474.6.
Step 1: (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropyl-1-methylpiperazine
A procedure analogous to Example 48, Step 3 using (R)-2-isopropyl-1-methylpiperazine-2TFA (0.500 g, 1.35 mmol), 4-bromo-1-chloro-2-fluorobenzene (0.196 mL, 1.69 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.037 g, 0.041 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.070 g, 0.122 mmol) and Cs2CO3 (2.64 g, 8.10 mmol) afforded the product (167 mg, 46%). LCMS: [M+H]+=271.09.
Step 2: (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropyl-1-methylpiperazine
A procedure analogous to Example 48, Step 4 using (R)-4-(4-chloro-3-fluorophenyl)-2-isopropyl-1-methylpiperazine (0.167 g, 0.617 mmol), bis(pinacolato)diboron (0.235 g, 0.925 mmol), KOAc (0.121 g, 1.23 mmol) and XPhos Pd G2 (0.036 g, 0.046 mmol) afforded the product (263 mg, quantitative yield). LCMS [M+H]+=363.30 Step 3: (R)-6-(2-amino-6-fluoro-5-(2-fluoro-4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 4, Step 5 using 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.037 g, 0.164 mmol), (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropyl-1-methylpiperazine (0.065 g, 0.181 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.013 g, 0.016 mmol) afforded the product (14 mg, 22%). LCMS: [M+H]+=381.20.
Step 4: (R)-6-(2-amino-6-fluoro-5-(2-fluoro-4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.012 g, 0.042 mmol), (R)-3-chloro-6-fluoro-5-(2-fluoro-4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (0.014 g, 0.037 mmol) and XPhos Pd G2 (2.89 mg, 3.68 μmol) and aqueous K3PO4 (0.085 mL of a 1.3 M solution, 0.110 mmol) afforded the title compound (5 mg, 26%) as a pale yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.3-7.4 (m, 3H), 7.20 (t, J=8.7 Hz, 1H), 6.74 (br d, J=11.5 Hz, 2H), 6.19 (s, 2H), 3.55 (br d, J=11.9 Hz, 1H), 3.43 (br d, J=11.9 Hz, 1H), 3.33 (dt, J=6.5, 2.9 Hz, 2H), 2.87 (br t, J=6.5 Hz, 2H), 2.8-2.8 (m, 1H), 2.69 (dt, J=11.8, 2.9 Hz, 1H), 2.21 (dt, J=11.6, 3.1 Hz, 1H), 2.12 (s, 3H), 2.05 (qd, J=11.1, 6.9 Hz, 1H,), 1.85 (td, J=10.7, 3.4 Hz, 1H), 0.92 (d, J=7.0 Hz, 3H), 0.81 (d, J=7.0 Hz, 3H); LCMS [M+H]+=492.4.
Step 1: tert-butyl (S)-3-isopropyl-4-methylpiperazine-1-carboxylate
A procedure analogous to Example 48, Step 1 using (S)-1-Boc-3-isopropyl-piperazine (1.0 g, 4.38 mmol) (1.0 g, 4.38 mmol) in MeOH/THF (10 mL each), formaldehyde solution, 37% wt in water (0.489 ml, 6.57 mmol) and NaBH(OAC)3 (1.39 g, 6.57 mmol) afforded the product (1.09 g, quantitative yield) as a clear oil. LCMS: [M+H]=243.42.
Step 2: (S)-2-isopropyl-1-methylpiperazine 2TFA
A procedure analogous to Example 48, Step 2 using TFA (1.97 mL, 25.8 mmol), tert-butyl (S)-3-isopropyl-4-methylpiperazine-1-carboxylate (0.50 g, 2.06 mmol) in CH2Cl2 (10 mL) at RT afforded the product (1.05 g, quantitative yield). LCMS: [M+H]+=143.42.
Step 3: (S)-4-(4-chlorophenyl)-2-isopropyl-1-methylpiperazine
A procedure analogous to Example 48, Step 3 using (S)-2-isopropyl-1-methylpiperazine-2TFA (0.500 g, 1.35 mmol), 1-chloro-4-iodobenzene (0.402 g, 1.69 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.037 g, 0.041 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.070 g, 0.122 mmol) and Cs2CO3 (2.64 g, 8.10 mmol) afforded the product (150 mg, 44%). LCMS: [M+H]+=253.27.
Step 4: ((S)-2-isopropyl-1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine
A procedure analogous to Example 48, Step 4 using (S)-4-(4-chlorophenyl)-2-isopropyl-1-methylpiperazine (0.150 g, 0.593 mmol), bis(pinacolato)diboron (0.226 g, 0.890 mmol), KOAc (0.116 g, 1.19 mmol) and XPhos Pd G2 (0.035 g, 0.045 mmol) afforded the product (158 mg, 77%). LCMS: [M+H]+=345.32
Step 5: (S)-6-(2-amino-6-fluoro-5-(4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 48, Step 5 using 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.045 g, 0.200 mmol), (S)-2-isopropyl-1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (0.076 g, 0.220 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.016 g, 0.020 mmol) afforded the product (29 mg, 40%). LCMS: [M+H]+=363.30.
Step 6: (S)-6-(2-amino-6-fluoro-5-(4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.025 g, 0.092 mmol), (S)-3-chloro-6-fluoro-5-(4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (0.029 g, 0.080 mmol) and XPhos Pd G2 (6.29 mg, 7.99 μmol) and aqueous K3PO4 (0.184 mL of a 1.3 M solution, 0.240 mmol) afforded the title compound (8 mg, 20%) as a yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.51 (d, J=10.1 Hz, 1H), 7.4-7.4 (m, 2H), 7.33 (br d, J=7.8 Hz, 2H), 6.90 (d, J=8.9 Hz, 2H), 6.10 (br s, 2H), 3.5-3.5 (m, 1H), 3.41 (br d, J=11.7 Hz, 1H), 3.34 (dt, J=6.4, 2.9 Hz, 2H), 2.88 (br t, J=6.5 Hz, 2H), 2.8-2.8 (m, 1H), 2.65 (dt, J=11.7, 2.9 Hz, 1H), 2.23 (dt, J=11.5, 3.0 Hz, 1H), 2.12 (s, 3H), 2.05 (dt, J=6.9, 4.3 Hz, 1H), 1.87 (td, J=10.7, 3.4 Hz, 1H), 0.92 (d, J=7.0 Hz, 3H), 0.81 (d, J=7.0 Hz, 3H); LCMS: [M+H]+=474.5.
Step 1: (S)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropyl-1-methylpiperazine
A procedure analogous to Example 48, Step 3 using (S)-2-isopropyl-1-methylpiperazine-2TFA (0.500 g, 1.35 mmol), 4-bromo-1-chloro-2-fluorobenzene (0.196 mL, 1.69 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.037 g, 0.041 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.070 g, 0.122 mmol) and Cs2CO3 (2.64 g, 8.10 mmol) afforded the product (178 mg, 49%). LCMS: [M+H]+=271.01.
Step 2: (S)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropyl-1-methylpiperazine
A procedure analogous to Example 43, Step 4 using (S)-4-(4-chloro-3-fluorophenyl)-2-isopropyl-1-methylpiperazine (0.178 g, 0.657 mmol), bis(pinacolato)diboron (0.250 g, 0.986 mmol), KOAc (0.129 g, 1.315 mmol) and XPhos Pd G2 (0.039 g, 0.049 mmol) afforded the product (252 mg, quantitative yield). LCMS: [M+H]+=363.22.
Step 3: (S)-3-chloro-6-fluoro-5-(2-fluoro-4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-2-amine
A procedure analogous to Example 43, Step 5 using 5-bromo-3-chloro-6-fluoropyridin-2-amine (0.035 g, 0.155 mmol), (S)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropyl-1-methylpiperazine (0.062 g, 0.171 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (0.013 g, 0.016 mmol) afforded the product (17 mg, 29%). LCMS: [M+H]+=381.28.
Step 4: (S)-6-(2-amino-6-fluoro-5-(2-fluoro-4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.014 g, 0.051 mmol), (S)-3-chloro-6-fluoro-5-(2-fluoro-4-(3-isopropyl-4-methylpiperazin-1-yl)phenyl)pyridin-2-amine (0.017 g, 0.045 mmol), XPhos Pd G2 (3.51 mg, 4.46 μmol) and aqueous K3PO4 (0.103 mL of a 1.3 M solution, 0.134 mmol) afforded the title compound (4 mg, 17%) as a yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.3-7.4 (m, 3H), 7.20 (t, J=8.7 Hz, 1H), 6.74 (br d, J=11.4 Hz, 2H), 6.19 (s, 2H), 3.55 (br d, J=11.7 Hz, 1H), 3.43 (br d, J=11.7 Hz, 1H), 3.33 (td, J=6.3, 3.2 Hz, 2H), 2.87 (br t, J=6.4 Hz, 2H), 2.78 (br d, J=11.4 Hz, 1H), 2.69 (dt, J=11.8, 2.6 Hz, 1H), 2.21 (dt, J=11.6, 3.0 Hz, 1H), 2.12 (s, 3H), 2.05 (qd, J=11.1, 6.8 Hz, 1H), 1.85 (td, J=10.5, 3.2 Hz, 1H), 0.92 (d, J=6.8 Hz, 3H), 0.81 (d, J=6.8 Hz, 3H); LCMS: [M+H]+=492.5.
Step 1: 3-(4-bromophenyl)-1-ethylpiperidine
To a slurry of 3-(4-bromo-phenyl)-piperidine hydrochloride (300 mg, 1.09 mmol) in DMF (5 mL) at RT was added K2CO3 (600 mg, 4.34 mmol) and bromoethane (0.121 ml, 1.63 mmol) and the mixture was stirred at RT for 1.5 h. The reaction mixture was concentrated to dryness, taken up in DCM/water, and the organic phase was separated. The aqueous phase was extracted with DCM (x 2), the combined organic layers were washed (x 3) with water and with brine, dried over Na2SO4 and concentrated to yield the product. LCMS: [M+H]+=269.98.
Step 2: 6-(2-amino-5-(4-(1-ethylpiperidin-3-yl) phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A mixture of 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (138 mg, 0.287 mmol) (3.5 mL of the stock solution of 40 mg/mL), Cs2CO3 (200 mg, 0.615 mmol), 3-(4-bromophenyl)-1-ethylpiperidine (55 mg, 0.205 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (22.51 mg, 0.031 mmol) and water (1 mL) in a microwave vial was flushed with Argon and heated in a microwave reactor at 90° C. for 1 h. The reaction mixture was partitioned between brine and EtOAc, the organic phase was separated and the aqueous phase was extracted with EtOAc. The combined organic layers were dried over Na2SO4 and concentrated onto Celite®. Purification by reverse phase flash chromatography on a RediSep column (5.5 G), eluting with water containing 0-35% acetonitrile, afforded the title compound was isolated as a beige solid (54 mg, 56%). 1H NMR (500 MHz, CD3OD) δ 8.0-8.1 (m, 1H), 7.6-7.7 (m, 1H), 7.4-7.5 (m, 4H), 7.3-7.3 (m, 2H), 3.5-3.6 (m, 2H), 3.1-3.2 (m, 2H), 3.0-3.1 (m, 2H), 2.8-2.9 (m, 1H), 2.57 (q, J=6.8 Hz, 2H), 2.1-2.2 (m, 2H), 1.9-2.0 (m, 1H), 1.9-1.9 (m, 1H), 1.7-1.8 (m, 1H), 1.5-1.6 (m, 1H), 1.15 (t, J=7.2 Hz, 3H); LCMS: [M+H]+=445.16.
Step 1: 4-(4-bromophenyl)-1-ethylpiperidine
To a slurry of 4-(4-bromophenyl)piperidine (520 mg, 2.165 mmol) in DMF (6 mL) at RT, was added K2CO3 (898 mg, 6.50 mmol) followed by bromoethane (0.241 mL, 3.25 mmol), and the reaction mixture was stirred at RT for 1.5 h. The mixture was concentrated to dryness and the residue was partitioned between CH2Cl2 and water. The layers were separated and the aqueous layer was extracted with CH2Cl2 (x2). The combined organic phase was washed with water, brine, dried over Na2SO4 and concentrated to yield the title compound as a light yellow oil (274 mg, 47%). As the purity was good, it was taken to the next step without any purification. LCMS: [M+H]+=268.39.
Step 2: 6-(2-amino-5-(4-(1-ethylpiperidin-4-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 52, Step 2 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (147 mg, 0.308 mmol) and 4-(4-bromophenyl)-1-ethylpiperidine (55 mg, 0.205 mmol) afforded the title compound as a beige solid (30 mg, 31%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.6-7.7 (m, 1H), 7.5-7.5 (m, 4H), 7.3-7.3 (m, 2H), 6.3-6.3 (m, 2H), 3.4-3.4 (m, 2H), 3.2-3.3 (m, 1H), 3.1-3.2 (m, 2H), 2.9-3.0 (m, 2H), 2.4-2.5 (m, 4H), 1.8-1.9 (m, 2H), 1.7-1.8 (m, 2H), 1.1-1.1 (m, 3H). LCMS: [M+H]+=445.41.
To a suspension of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24.9 mg, 0.058 mmol) and N,N-diisopropylethylamine (0.030 ml, 0.173 mmol) in CH2Cl2 (3 mL) was added methanesulfonyl chloride (8.91 μl, 0.115 mmol) at RT. The reaction mixture was stirred for 1 h at 23° C. The reaction mixture was dry loaded and purified using flash chromatography (0-20%, MeOH/DCM) to afford the title compound (24.1 mg, 78%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.61 (d, J=10.1 Hz, 1H), 7.52-7.44 (m, 4H), 7.04 (d, J=8.8 Hz, 2H), 6.24 (s, 2H), 4.65-4.57 (m, 1H), 3.41 (br d, J=2.4 Hz, 2H), 3.39-3.33 (m, 2H), 3.18-3.11 (m, 2H), 2.99-2.94 (m, 2H), 2.91 (s, 3H), 2.01 (br s, 2H), 1.77 (br s, 2H); LCMS: [M+H]+=511.28.
Step 1: 3-chloro-6-fluoro-5-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)pyridin-2-amine
A procedure analogous to Example 47, Step 3 using 5-bromo-3-chloro-6-fluoropyridin-2-amine (150 mg, 0.665 mmol), PdCl2dppf (48.7 mg, 0.067 mmol), 1-(tetrahydro-pyran-4-yl)-1H-pyrazole-4-boronic acid pinacol ester (185 mg, 0.665 mmol), Cs2CO3 (650 mg, 2.00 mmol), H2O (5 mL) and DME (10 mL) the product as a dark brown solid (311 mg, 95% yield based on 60% purity). LCMS: [M+H]+=297.11.
Step 2: 6-(2-Amino-6-fluoro-5-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 1, General Method A using 3-chloro-6-fluoro-5-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)pyridin-2-amine (155 mg, 0.313 mmol, purity of 60%), K3PO4 (200 mg, 0.940 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (111 mg, 0.407 mmol), H2O (6 mL), MeCN (9 mL) and XPhos Pd G2 (24.7 mg, 0.031 mmol) afforded the title compound as an off white solid (9 mg, 7%). 1H NMR (500 MHz, CDC3) δ 8.11 (d, J=8.0 Hz, 1H), 7.66-7.74 (m, 2H), 7.57 (d, J=9.3 Hz, 1H), 7.38-7.41 (m, 1H), 7.26 (s, 1H), 5.96-6.09 (m, 1H), 4.53-4.64 (m, 2H), 4.24-4.38 (m, 1H), 4.01-4.10 (m, 2H), 3.41-3.61 (m, 4H), 2.96-3.05 (m, 2H), 2.01-2.12 (m, 4H). LCMS: [M+H]+=408.28.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (300 mg, 0.892 mmol) and (4-(1-(tert-butoxycarbonyl)-1,2,3,6-tetrahydropyridin-4-yl)phenyl)boronic acid pinacol ester (447 mg, 1.16 mmol) afforded the title compound as a beige solid (353 mg, 73%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 2H), 7.4-7.5 (m, 4H), 6.3-6.4 (m, 2H), 6.2-6.3 (m, 1H), 4.0-4.1 (m, 2H), 3.5-3.6 (m, 2H), 3.4-3.4 (m, 2H), 3.3-3.4 (m, 2H), 2.9-3.0 (m, 2H), 1.4-1.5 (m, 9H); LCMS: [M+H]+=515.49.
Tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-3,6-dihydropyridine-1(2H)-carboxylate (141 mg, 0.215 mmol, prepared in Example 56) was stirred at RT with CH2Cl2 (2 mL) and TFA (0.5 mL) for 1 h. The reaction mixture was concentrated onto Celite® and purification by reverse phase chromatography (C18; 0-10% CH3CN/water) afforded the title compound as an off white solid (68 mg, 57%). 1H NMR (500 MHz, DMSO-d6) δ 8.93-8.70 (m, 2H), 8.01-7.94 (m, 1H), 7.94-7.89 (m, 1H), 7.73-7.65 (m, 1H), 7.63-7.57 (m, 2H), 7.57-7.51 (m, 2H), 7.50-7.45 (m, 2H), 6.44-6.31 (m, 2H), 6.31-6.23 (m, 1H), 3.84-3.76 (m, 2H), 3.45-3.40 (m, 2H), 3.39-3.36 (m, 2H), 3.00-2.93 (m, 2H), 2.75-2.68 (m, 2H). LCMS: [M+H]+=415.45.
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (114 mg, 0.339 mmol) and 4-methyl-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydro-2H-1,4-benzoxazine (112 mg, 0.407 mmol) afforded the title compound (95 mg, 69%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.54 (d, J=10.1 Hz, 1H), 7.46 (d, J=7.9 Hz, 1H), 7.44 (s, 1H), 6.97 (br d, J=8.4 Hz, 1H), 6.87 (s, 1H), 6.72 (d, J=8.4 Hz, 1H), 6.15 (s, 2H), 4.27-4.20 (m, 2H), 3.40 (td, J=6.5, 2.7 Hz, 3H), 3.27-3.21 (m, 3H), 2.99-2.91 (m, 3H), 2.84 (s, 4H); LCMS: [M+H]+=405.13.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (120 mg, 0.357 mmol) and 1-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]pyrrolidine (117 mg, 0.428 mmol) afforded the title compound (11 mg, 8%) as a brown solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.55 (d, J=10.1 Hz, 1H), 7.46 (br d, J=7.9 Hz, 1H), 7.44 (s, 1H), 7.35 (br d, J=8.1 Hz, 2H), 6.57 (d, J=8.7 Hz, 2H), 6.10 (s, 2H), 3.43-3.38 (m, 2H), 3.23 (br t, J=6.2 Hz, 4H), 2.95 (br t, J=6.4 Hz, 2H), 1.95 (br t, J=6.3 Hz, 4H); LCMS: [M+H]+=403.10.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (93 mg, 0.277 mmol) and 1-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]piperidine (95 mg, 0.332 mmol) afforded the title compound (77 mg, 66%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.58 (d, J=10.3 Hz, 1H), 7.48-7.45 (m, 1H), 7.44 (s, 1H), 7.38 (d, J=7.8 Hz, 2H), 6.95 (d, J=8.9 Hz, 2H), 6.16 (s, 2H), 3.40 (td, J=6.5, 2.8 Hz, 2H), 3.19-3.14 (m, 4H), 2.95 (t, J=6.5 Hz, 2H), 1.64-1.58 (m, 4H), 1.57-1.51 (m, 2H); LCMS: [M+H]+=418.37.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (95 mg, 0.283 mmol) and 2-chloro-4-(N-methylpiperazin-1-yl)phenylboronic acid (86 mg, 0.339 mmol) to provide the title compound (78 mg, 59%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.89 (d, J=7.8 Hz, 1H), 7.45-7.38 (m, 3H), 7.24 (d, J=8.7 Hz, 1H), 7.03 (d, J=2.3 Hz, 1H), 6.95 (dd, J=8.7, 2.4 Hz, 1H), 6.28 (s, 2H), 3.39 (td, J=6.5, 2.6 Hz, 2H), 3.21-3.18 (m, 4H), 2.94 (t, J=6.5 Hz, 2H), 2.45-2.42 (m, 4H), 2.22 (s, 3H); LCMS: [M+1]+=465.99.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (105 mg, 0.312 mmol) and 4-[(methylsulfonyl)phenylboronic acid (75 mg, 0.375 mmol) to provide the title compound (0.245 mmol) in 78% yield as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.89 (m, 4H), 7.85 (br d, J=7.3 Hz, 2H), 7.76 (br d, J=9.7 Hz, 1H), 7.53-7.42 (m, 2H), 6.54 (br s, 2H), 3.41 (br s, 2H), 3.24 (br s, 3H), 2.96 (br s, 2H); LCMS: [M+H]+=412.19.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (87 mg, 0.259 mmol) and 4-(cyclopropylsulfonyl)phenylboronic acid (70 mg, 0.311 mmol) afforded the title compound (98 mg, 83%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.93-7.89 (m, 3H), 7.86-7.82 (m, 2H), 7.77 (d, J=10.1 Hz, 1H), 7.49 (d, J=8.1 Hz, 1H), 7.46 (s, 1H), 6.54 (s, 2H), 3.41 (td, J=6.4, 2.7 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.88 (tt, J=7.9, 4.8 Hz, 1H), 1.16-1.11 (m, 2H), 1.08-1.02 (m, 2H); LCMS: [M+H]+=438.30.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (98 mg, 0.292 mmol) and 4-(diethylsulfamoyl)benzeneboronic acid (84 mg, 0.328 mmol) afforded the title compound (120 mg, 93%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.92 (d, J=7.9 Hz, 1H), 7.84 (d, J=8.1 Hz, 2H), 7.77 (d, J=8.7 Hz, 3H), 7.48 (d, J=7.9 Hz, 1H), 7.46 (s, 1H), 6.54 (s, 2H), 3.41 (td, J=6.4, 2.8 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.63 (s, 6H); LCMS: [M +1]+=441.31.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (92 mg, 0.274 mmol) and 4-(diethylsulfamoyl)benzeneboronic acid (84 mg, 0.328 mmol) afforded the title compound (130 mg, 83%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.82-7.77 (m, 4H), 7.75 (d, J=10.1 Hz, 1H), 7.48 (br d, J=7.9 Hz, 1H), 7.46 (s, 1H), 6.52 (s, 2H), 3.41 (td, J=6.4, 2.6 Hz, 2H), 3.18 (q, J=7.1 Hz, 4H), 2.95 (br t, J=6.5 Hz, 2H), 1.06 (t, J=7.1 Hz, 6H); LCMS: [M+1]+=469.22.
Step 1: (1R, 2S)-1-(4-bromophenyl)-2-(hydroxymethyl)cyclopropane-1-carbonitrile:
To a stirred solution of 2-(4-bromophenyl)acetonitrile (10 g, 51 mmol) in THF (80 mL), was added NaHMDS (63.7 mL, 127.5 mmol, 2M in THF) at −10° C. The mixture was stirred for 10 min, then epichlorohydrin (5.19 g, 56.1 mmol) was added dropwise at −10° C. The reaction mixture was stirred at RT for 4 h, then quenched with 2:1 dioxane: conc. HCl (10 mL) and extracted with Et2O (3×100 mL). The combined organic layers were dried over Na2SO4 and concentrated under reduced pressure to afford the product (12 g, quantitative yield) as a pale yellow semi solid. LCMS: [M+H]+=253.23.
Step 2: ((1S, 2R)-2-(aminomethyl)-2-(4-bromophenyl) cyclopropyl) methanol:
To a stirred solution of (1R, 2S)-1-(4-bromophenyl)-2-(hydroxymethyl)cyclopropane-1-carbonitrile (crude 7.1 g, 28.2 mmol) in THF (70 mL) was added BF3·OEt2 (5.6 mL, 5.6 mmol) at RT. The reaction mixture was heated to 80° C., then added BH3 DMS (70.7 mL, 70.7 mmol, 1M in THF). The reaction mixture was maintained at 80° C. for 4 h. Then, the reaction mixture was quenched with 10% aq.HCl (30 mL), washed with diethyl ether (2×200 mL) and the aqueous layer was neutralized with solid NaHCO3 up to PH-8 and extracted with EtOAc (3×200 mL), the combined organic layer was wash with brine solution (200 mL), dried over with Na2SO4, filtered and concentrated under reduced pressure to afford the product (4.5 g, 62%) as pale yellow semi solid. LCMS: [M+H]+=256.26.
Step 3: (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane
To a stirred solution of ((1S, 2R)-2-(aminomethyl)-2-(4-bromophenyl) cyclopropyl) methanol (4 g, 15.6 mmol) in DCM (40 mL) was added thionyl chloride (3.5 mL, 47.0 mmol) dropwise at 0° C. The reaction mixture was stirred for 16 h at RT and washed with CH2Cl2 (2×100 mL). The aqueous layer was basified with sat. aqueous Na2CO3 solution to pH 8, extracted with 10% MeOH in DCM (3×200 mL), and the combined organic layers were dried over Na2SO4 and concentrated to gave crude product. Trituration with EtOAc afforded the product (3 g, 81%) as a white solid. LCMS: [M+H]+=238.17.
Step 4: (1R, 5S)-1-(4-bromophenyl)-3-(tetrahydro-2H-pyran-4-yl)-3-azabicyclo [3.1.0]hexane (5):
A solution of (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (4 g, 16.8 mmol) and tetrahydro-4H-pyran-4-one (2.53 g, 25.3 mmol) in MeOH: ACOH (76:4 mL) was stirred for 30 min, then NaCNBH3 (2.1 g, 33.7 mmol) was added portionwise at RT. The reaction mixture was stirred at RT for 16 h, then concentrated and diluted with water and basified with aq sat.NaHCO3 solution to pH=8. The aqueous layer was separated and extracted with EtOAc (3×200 mL). The combined organic layers were dried over Na2SO4 and concentrated to give crude compound. Purification by column chromatography using 100-200 silica gel as an eluent EtOAc afforded the product (2 g, 37%) as an off-white semi solid. LCMS: [M+H]+=322.37
Step 5: (1R,5S)-3-(tetrahydro-2H-pyran-4-yl)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane:
To a stirred solution of (1R, 5S)-1-(4-bromophenyl)-3-(tetrahydro-2H-pyran-4-yl)-3-azabicyclo [3.1.0] hexane (5) (1 g, 3.1 mmol) and 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (3.8 mL, 18.6 mmol) in THF (50 mL) was added dropwise n-BuLi (11.6 mL, 18.6 mmol) at −78° C. The reaction mixture was maintained at RT for 6 h, quenched with aq sat. NH4Cl solution and extracted with EtOAc (3×50 mL). The combined organic layers were washed with brine solution (100 mL), dried over with Na2SO4, filtered and concentrated under reduced pressure to give crude compound. The crude compound was purified by prep HPLC to afford the product (0.2 g, 17%) as a white solid, which consisted of a mixture of boronic acid as well as boronate ester. 1H NMR (400 MHz, DMSO-d6): δ 7.57 (d, J=7.6 Hz, 2H), δ 7.14 (d, J=7.2 Hz, 2H), δ 3.82 (d, J=9.6 Hz, 4H), δ 3.36-3.26 (m, 4H), δ 3.06 (d, J=8.4 Hz, 2H), δ 2.55 (d, J=7.6 Hz, 2H), δ 2.42 (d, J=6.0 Hz, 1H), δ 2.31 (s, 1H), δ 1.83-1.70 (m, 2H), δ 1.43-1.22 (m, 12H); LCMS: [M+H]+=370.32.
Step 6: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-(tetrahydro-2H-pyran-4-yl)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 1, General Method A using (1R,5S)-3-(tetrahydro-2H-pyran-4-yl)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (69 mg, 0.187 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (62.8 mg, 0.187 mmol), PdCl2dppf (19.14 mg, 0.026 mmol), Cs2CO3 (183 mg, 0.561 mmol), H2O (2 mL) and 1,4-dioxane (4 mL) afforded the title compound as a light tan solid (57 mg, 60% yield based on purity of 98%). 1H NMR (500 MHz, DMSO-d6) δ 7.92-7.97 (m, 1H), 7.91 (d, J=8.0 Hz, 1H), 7.62 (d, J=10.2 Hz, 1H), 7.43-7.50 (m, 4H), 7.20 (d, J=8.2 Hz, 2H), 6.29 (s, 2H), 3.78-3.89 (m, 2H), 3.36-3.46 (m, 3H), 3.27-3.31 (m, 1H), 3.04-3.16 (m, 1H), 2.96 (br t, J=6.5 Hz, 2H), 2.53-2.62 (m, 2), 2.38-2.48 (m, 1H), 2.28-2.36 (m, 1H), 1.70-1.87 (m, 3H), 1.27-1.43 (m, 3H), 0.73-0.81 (m, 1H); LCMS: [M+H]+=499.41.
Step 1: 3-chloro-5-(4-(4-ethylpiperazin-1-yl)phenyl)-6-fluoropyridin-2-amine
A method similar to Example 1, General Method A heating a sealed and degassed mixture of 5-bromo-3-chloro-6-fluoropyridin-2-amine (70 mg, 0.310 mmol), (4-(4-ethylpiperazin-1-yl)phenyl)boronic acid pinacol ester (98 mg, 0.310 mmol), PdCl2dppf (22.7 mg, 0.031 mmol), Cs2CO3 (303 mg, 0.931 mmol) in H2O (2 mL) and DME (3 mL) under microwave irradiation at 90° C. for 1 h afforded the product as a brown solid (82 mg, 79%). LCMS: [M+H]+=335.00.
Step 2: 7-(2-amino-5-(4-(4-ethylpiperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-2,3,4,5-tetrahydro-1H-benzo[c]azepin-1-one
A procedure analogous to Example 43, Step 5 using 7-(tetramethyl-1, 3,2-dioxaborolan-2-yl)-2,3,4,5-tetrahydro-1H-2-benzazepin-1-one (77 mg, 0.27 mmol), K3PO4 (156 mg, 0.735 mmol), XPhos Pd G2 (19.3 mg, 0.024 mmol), H2O (6 mL), 3-chloro-5-(4-(4-ethylpiperazin-1-yl)phenyl)-6-fluoropyridin-2-amine (82 mg, 0.24 mmol) and MeCN (9 mL) afforded the product as a white solid (5 mg, 4% based on purity of 96%). 1H NMR (500 MHz, DMSO-d6) δ 8.18-8.28 (m, 2H), 7.75 (d, J=8.1 Hz, 1H), 7.56 (dd, J=8.1, 1.8 Hz, 1H), 7.52 (d, J=1.5 Hz, 1H), 7.47 (br d, J=7.5 Hz, 2H), 7.05 (d, J=8.8 Hz, 2H), 3.15-3.27 (m, 4H), 2.93-3.09 (m, 2H), 2.74-2.90 (m, 2H), 2.53-2.57 (m, 4H), 2.38 (q, J=7.0 Hz, 2H), 1.90-2.01 (m, 2H), 1.05 (t, J=7.2 Hz, 3H); LCMS: [M+H]+=460.36.
To a solution of 1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2-(trifluoromethyl)phenyl)piperazine (65.5 mg, 0.177 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (54.1 mg, 0.161 mmol) in 1,4-dioxane (3 mL) was added a solution of Cs2CO3 (157 mg, 0.483 mmol) in water (1 mL) at RT and the reaction mixture was degassed under argon for 15 min. Then PddppfCl2 (17.66 mg, 0.024 mmol) was added and the reaction mixture was heated at 90° C. for 6 h. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-20%, DCM/MeOH) to afford the title compound (66.2 mg, 78%) as a light brown powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.82 (br d, J=8.4 Hz, 1H), 7.78 (s, 1H), 7.71 (d, J=10.3 Hz, 1H), 7.58 (d, J=8.3 Hz, 1H), 7.47 (br d, J=8.1 Hz, 1H), 7.45 (s, 1H), 6.40 (s, 2H), 3.40 (br d, J=2.3 Hz, 2H), 2.95 (s, 2H), 2.93-2.87 (m, 4H), 2.26 (br s, 3H), 4H under the water peak; LCMS: [M+H]+=500.16.
A procedure similar to Example 1, General Method A using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,4-dimethyl-3,4-dihydroisoquinolin-1(2H)-one (132 mg, 0.301 mmol, purity of 83%), (4-(4-ethylpiperazin-1-yl)phenyl)boronic acid pinacol ester (100 mg, 0.316 mmol), PdCl2dppf (22.0 mg, 0.030 mmol), Cs2CO3 (294 mg, 0.902 mmol), H2O (6 mL) and DME (12 mL) afforded the title compound as an off white solid (26 mg, 18% based on purity of 96%). 1H NMR (500 MHz, DMSO-d6) δ 7.98 (br s, 1H), 7.89-7.96 (m, 1H), 7.59 (d, J=10.3 Hz, 1H), 7.45-7.53 (m, 2H), 7.41 (br d, J=8.0 Hz, 2H), 6.98 (d, J=8.9 Hz, 2H), 6.14 (s, 2H), 3.11-3.24 (m, 7H), 2.31-2.44 (m, 2H), 1.32 (s, 6H), 1.04 (t, J=7.2 Hz, 3H). Signals due to 3 H are obscured by H2O peak and/or the solvent peak. LCMS: [M+H]+=474.35.
A procedure analogous to Example 1, General Method A using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,3-dimethyl-3,4-dihydroisoquinolin-1(2H)-one (192 mg, 0.285 mmol, 54%), (4-(4-ethylpiperazin-1-yl)phenyl)boronic acid pinacol ester (95 mg, 0.30 mmol), PdCl2dppf (20.8 mg, 0.028 mmol), Cs2CO3 (278 mg, 0.854 mmol), H2O (6 mL), DME (12 mL) afforded the title compound as a pale yellow solid (67 mg, 39% yield based on purity of 98%). 1H NMR (500 MHz, CD3OD) δ ppm 8.05 (d, J=8.0 Hz, 1H), 7.64 (d, J=9.8 Hz, 1H), 7.47-7.54 (m, 3H), 7.40-7.47 (m, 1H), 7.10 (d, J=8.8 Hz, 2H), 3.93 (br d, J=12.5 Hz, 2H), 3.70 (br d, J=10.5 Hz, 2H), 3.28-3.32 (m, 2H), 3.19-3.27 (m, 2H), 3.07-3.16 (m, 2H), 3.02-3.05 (m, 2H), 1.42 (t, J=7.3 Hz, 3H), 1.36 (s, 6H). LCMS: [M+H]+=474.05.
6-(2-amino-5-(4-((1R,5S)-3-ethyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Step 1: (1R,5S)-1-(4-bromophenyl)-3-ethyl-3-azabicyclo[3.1.0]hexane
A procedure analogous to Example 53, Step 1 using (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (113 mg, 0.475 mmol, preparation described in Example 66, Step 3) and bromoethane (0.053 ml, 0.712 mmol) afforded the product as a white powder (94 mg, 74%). LCMS: [M+H]+=266.08.
Step 2: 6-(2-Amino-5-(4-((1R,5S)-3-ethyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using (1R,5S)-1-(4-bromophenyl)-3-ethyl-3-azabicyclo[3.1.0]hexane (94 mg, 0.353 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (176 mg, 0.459 mmol) afforded the title compound as a light brown oil (64 mg, 39%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 4H), 7.19 (d, J=8.4 Hz, 2H), 6.29 (s, 2H), 3.4-3.5 (m, 2H), 3.0-3.1 (m, 1H), 2.9-3.0 (m, 2H), 2.4-2.5 (m, 4H), 2.4-2.4 (m, 1H), 1.7-1.8 (m, 1H), 1.3-1.4 (m, 1H), 1.0-1.1 (m, 3H), 0.7-0.8 (m, 1H); LCMS: [M+H]+=443.41.
Step 1: (1R,5S)-1-(4-bromophenyl)-3-(2,2-difluoroethyl)-3-azabicyclo[3.1.0]hexane
A procedure analogous to Example 53, Step 1 using (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (107 mg, 0.449 mmol) and 2,2-difluoroethyl trifluoromethanesulfonate (0.120 ml, 0.899 mmol) at RT overnight. The reaction mixture was concentrated to Celite® and purified by reverse phase chromatography (C18; 0-60% acetonitrile/water) to afford the product as a light brown oil (87 mg, 64%). LCMS: [M+H]+=302.00
Step 2: 6-(2-amino-5-(4-((1R,5S)-3-(2,2-difluoroethyl)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 43, Step 5 using (1R,5S)-1-(4-bromophenyl)-3-(2,2-difluoroethyl)-3-azabicyclo[3.1.0]hexane (87 mg, 0.288 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (143 mg, 0.374 mmol)) afforded the title compound as a beige solid (50 mg, 35%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.91 (d, 1H, J=7.8 Hz), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 4H), 7.1-7.2 (m, 2H), 6.3-6.4 (m, 2H), 6.0-6.2 (m, 1H), 3.4-3.4 (m, 3H), 3.1-3.1 (m, 1H), 2.9-3.0 (m, 4H), 2.7-2.8 (m, 1H), 2.6-2.7 (m, 1H), 1.84 (td, J=7.8, 3.9 Hz, 1H), 1.3-1.3 (m, 1H), 0.7-0.8 (m, 1H); LCMS: [M+H]+=479.45.
To a slurry of 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-oneTFA (40 mg, 0.076 mmol, preparation described in Example 57) in DMF (2 mL) at RT was added 2,2-difluoroethyl trifluoromethanesulfonate (0.020 mL, 0.151 mmol) and Et3N (0.105 mL, 0.757 mmol) and the mixture was stirred for 16 h at RT. The reaction mixture was partitioned between water and CHCl3/IPA 4:1 mix. The layers were separated and the aqueous layer was extracted with CHCl3/IPA 4:1 mix (2×3 mL). The combined organic extracts were dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-35% acetonitrile/water) afforded the title compound as a beige solid (8 mg, 20% yield based on purity of 90%). 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.90 (m, 2H), 7.69-7.65 (m, 1H), 7.56-7.51 (m, 2H), 7.50-7.44 (m, 4H), 6.36-6.07 (m, 4H), 3.44-3.40 (m, 2H), 3.29-3.22 (m, 2H), 2.99-2.95 (m, 2H), 2.91-2.82 (m, 2H), 2.82-2.76 (m, 2H), 2.54-2.52 (m, 2H); LCMS: [M+H]+=479.45.
Step 1: 6-(2-Amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a microwave vial containing a suspension of 5-bromo-6-chloro-3-iodopyridin-2-amine (121 mg, 0.362 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (90 mg, 0.330 mmol), K2CO3 (96 mg, 0.692 mmol) and bis(triphenylphosphine)palladium(II) dichloride (27.8 mg, 0.040 mmol) in DMF (9 mL) was added water (1 mL). The vial was flushed with argon, sealed and then heated in a microwave reactor at 80° C. for 1 h. The reaction mixture was partitioned between water and EtOAc. The organic phase was separated and aqueous phase was further extracted with DCM (2×3 mL). The combined org phase was dried and concentrated onto Celite®. Silica gel chromatography (eluting with 0-60% EtOAc/Hexanes) afforded the title compound as an off white solid (60 mg, 52%). LCMS: [M+H]+=354.21
Step 2: 6-(2-amino-6-chloro-5-(4-(4-methylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one:
A procedure analogous to that of Example 43, Step 5, by coupling 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (29 mg, 0.082 mmol) and 4-(4-methylpiperazin-1-yl)phenylboronic acid, pinacol ester (34.8 mg, 0.115 mmol) at 90° C. for 1.75 h. The reaction mixture was partitioned between brine and EtOAc. The organic phase was separated and aqueous phase was further extracted with EtOAc (2×3 mL). The combined organic layers were dried over Na2SO4 and concentrated onto Celite®. Reverse phase chromatography (C18; 0-30% acetonitrile/water), afforded the title compound as a yellow powder (19 mg, 39%). 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.92 (m, 1H), 7.89 (d, J=7.9 Hz, 1H), 7.48-7.43 (m, 2H), 7.38-7.34 (m, 1H), 7.34-7.27 (m, 2H), 7.00-6.94 (m, 2H), 6.22 (s, 2H), 3.43-3.37 (m, 2H), 3.19-3.15 (m, 4H), 2.94 (br t, J=6.5 Hz, 2H), 2.47-2.43 (m, 4H), 2.24-2.21 (m, 3H); LCMS: [M+H]+=448.48.
A procedure analogous to that of Example 43, Step 5, using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (25 mg, 0.071 mmol), prepared as per Example 74, Step 1) and (4-(4-ethylpiperazin-1-1)phenyl)boronic acid pinacol ester (31.4 mg, 0.099 mmol) at 90° C. for 1.75 h afforded the title compound as a pale yellow solid (16 mg, 46%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.92 (m, 1H), 7.92-7.86 (m, 1H), 7.50-7.45 (m, 1H), 7.45-7.42 (m, 1H), 7.37-7.34 (m, 1H), 7.31 (brd, J=8.4 Hz, 2H), 7.06-6.90 (m, 2H), 6.33-6.17 (m, 2H), 3.42-3.38 (m, 2H), 3.20-3.13 (m, 4H), 2.97-2.91 (m, 2H), 2.53-2.51 (m, 4H), 2.37 (q, J=7.1 Hz, 2H), 1.07-1.01 (m, 3H). LCMS: [M+H]+=462.40.
A slurry of 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one.TFA (30 mg, 0.057 mmol), bromoethane (8.42 μl, 0.114 mmol) and Et3N (0.079 mL, 0.568 mmol) in DMF (2 mL) was stirred overnight at RT. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x2). The combined organic extracts were dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-100% acetonitrile/water) afforded the title compound as a beige solid (15.5 mg, 59%). 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.70-7.64 (m, 1H), 7.56-7.51 (m, 2H), 7.50-7.43 (m, 4H), 6.39-6.25 (m, 2H), 6.24-6.16 (m, 1H), 3.46-3.39 (m, 2H), 3.07 (br d, J=2.6 Hz, 2H), 3.01-2.93 (m, 2H), 2.66-2.58 (m, 2H), 2.50-2.48 (m, 2H), 2.48-2.40 (m, 2H), 1.06 (t, J=7.1 Hz, 3H); LCMS: [M+H]+=443.41.
Step 1. 6-(2-amino-6-fluoropyridin-3-yl)-3,4-dihydro-2,7-naphthyridin-1(2H)-one
A microwave vial was charged with XPhos Pd G2 (110 mg, 0.139 mmol) 3-chloro-6-fluoropyridin-2-amine (120 mg, 0.819 mmol), B2pin2 (385 mg, 1.51 mmol), KOAc (281 mg, 2.87 mmol) and then filled with N2. 1,4-dioxane (10 mL) was added and the reaction was degassed with N2. The sealed vial was heated under microwave irradiation at 110° C. for 3 h. (LCMS [M+H]+ 238.74). The crude mixture containing 6-fluoro-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (approx. 73% purity) was then charged with 6-bromo-3,4-dihydro-2H-[2,7]naphthyridin-1-one (149 mg, 0.658 mmol), Cs2CO3(584 mg, 1.79 mmol), PdCl2dppf(43.8 mg, 0.060 mmol) and H2O (3 mL). The content was degassed with N2 and subsequently heated sealed under microwave irradiation for 1 h at 90° C. To drive the reaction to completion, another portion of 6-bromo-3,4-dihydro-2H-[2,7]naphthyridin-1-one (67.9 mg, 0.299 mmol) and PdCl2dppf(43.8 mg, 0.060 mmol) was added at RT. The reaction mixture was again degassed and subjected to heating sealed in a microwave reactor at 90° C. for 1 h. The reaction mixture was concentrated under reduced pressure and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2 and later 2 M NH3/MeOH in CH2Cl2), to afford the product as a beige solid (237 mg, purity of 77%). LCMS: [M+H]+=259.21.
Step 2.6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydro-2,7-naphthyridin-1(2H)-one:
To a suspension of 6-(2-amino-6-fluoropyridin-3-yl)-3,4-dihydro-2,7-naphthyridin-1(2H)-one (237 mg, 0.707 mmol, 77%) in DMF (12 mL) was added NBS (132 mg, 0.742 mmol) in one portion at 0° C. The reaction was allowed to warm slowly to RT and then stirred for the total of 2 h. The mixture was concentrated to dryness in vacuo, taken into MeCN (2 mL), sonicated and left at −20° C. overnight. A filtration of the cold mixture and washing of the solid with small amounts of MeCN afforded the product as an orange-rust solid (82 mg, 31% yield based on purity of 90%). LCMS: [M+H]+=337.11/339.14.
Step 3. (S)-6-(2-amino-6-fluoro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydro-2,7-naphthyridin-1(2H)-one, TFA
A procedure similar to Example 1, General Method A using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydro-2,7-naphthyridin-1(2H)-one (37 mg, 0.099 mmol, 90 %), (S)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine (58.9 mg, 0.178 mmol, from Example 5, Step 2), Cs2CO3 (113 mg, 0.346 mmol, PdCl2dppf (10.8 mg, 0.015 mmol), H2O (5 mL), EtOH (7.5 mL) and DME (7.5 mL) afforded the TFA salt of the title compound as a yellow solid (20 mg, 32%). 1H NMR (500 MHz, DMSO-d6) δ 8.96 (s, 1H), 8.32 (d, J=10.2 Hz, 1H), 8.06-8.14 (m, 2H), 7.80-8.03 (m, 1H), 7.48 (br d, J=8.1 Hz, 2H), 7.05 (d, J=8.8 Hz, 2H), 3.99 (br dd, J=11.2, 2.3 Hz, 1H), 3.59-3.67 (m, 2H), 3.56 (br d, J=11.9 Hz, 1H), 3.22-3.36 (m, 1H), 3.01 (br t, J=6.5 Hz, 2H), 2.69 (td, J=11.8, 3.3 Hz, 1H), 2.43-2.49 (m, 1H), 1.69-1.79 (m, 1H), 0.93-1.00 (m, 6H) one signal (7.80-8.03). Separate peaks corresponding to 3 H are obscured by the H2O and/or solvent peaks. LCMS [M+H]+=462.39.
Step 1: 6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3-(4-bromo-phenyl)-piperidine hydrochloride (175 mg, 0.633 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (315 mg, 0.823 mmol). The reaction was partitioned between water and CHCl3IPA 4:1 mix. The layers were separated and the aqueous layer was extracted with CHCl3/IPA 4:1 mix (2×3 mL). The combined organic extracts were dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-100% acetonitrile/water) afforded the product as a beige solid (192 mg, 66% yield based on purity of 90%). LCMS: [M+H]+=417.46.
Step 2: 6-(2-amino-5-(4-(1-(2,2-difluoroethyl)piperidin-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one:
A procedure analogous to that of Example 53, Step 1 using 6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.086 mmol) and 2,2-difluoroethyl trifluoromethanesulfonate (0.023 mL, 0.173 mmol) at 80° C. for 6 h. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x 2). The combined organic extracts was dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-30% acetonitrile/water) afforded the title compound as a beige solid (26 mg, 60%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.4-7.5 (m, 4H), 7.3-7.4 (m, 2H), 6.3-6.4 (m, 2H), 6.0-6.3 (m, 1H), 3.4-3.4 (m, 2H), 2.9-3.0 (m, 4H), 2.7-2.8 (m, 3H), 2.2-2.3 (m, 2H), 1.8-1.9 (m, 1H), 1.7-1.8 (m, 1H), 1.5-1.7 (m, 1H), 1.4-1.5 (m, 1H); LCMS: [M+H]+=481.45.
A procedure analogous to that of Example 43, Step 5 using 4-(4-bromophenyl)-1-isopropylpiperidine (40 mg, 0.135 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (67.1 mg, 0.175 mmol) afforded the title compound as a beige solid (25.5 mg, 39%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.67-7.61 (m, 1H), 7.52-7.44 (m, 4H), 7.29 (br d, J=8.1 Hz, 2H), 6.28 (br s, 2H), 3.45-3.39 (m, 2H), 3.01-2.93 (m, 2H), 2.92-2.85 (m, 2H), 2.77-2.68 (m, 1H), 2.49-2.43 (m, 1H), 2.23-2.22 (m, 1H), 2.22 (br t, J=11.1 Hz, 1H), 1.77 (br d, J=11.7 Hz, 2H), 1.70-1.56 (m, 2H), 1.08-0.92 (m, 6H); LCMS: [M+H]+=459.52.
A slurry of 6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.096 mmol, from Example 78, Step 1) and 2-iodopropane (0.029 mL, 0.288 mmol was heated at 70° C. for 1 h. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x2). The combined organic extracts were dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-50% acetonitrile/water) afforded the title compound as a beige solid (15.5 mg, 35%). 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.94 (m, 1H), 7.94-7.88 (m, 1H), 7.66-7.62 (m, 1H), 7.60-7.52 (m, 2H), 7.50-7.46 (m, 1H), 7.46-7.43 (m, 1H), 7.43-7.35 (m, 2H), 6.46-6.21 (m, 2H), 3.57-3.49 (m, 1H), 3.46-3.36 (m, 4H), 3.21-3.11 (m, 1H), 3.09-2.99 (m, 2H), 2.98-2.93 (m, 2H), 2.06-1.97 (m, 1H), 1.95-1.89 (m, 1H), 1.88-1.69 (m, 2H), 1.33-1.24 (m, 6H). LCMS: [M+H]+=459.58.
A slurry of 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.072 mmol) and 2-iodopropane (0.029 ml, 0.290 mmol) and Et3N (0.101 ml, 0.724 mmol) in DMF (2 mL) was heated at 70° C. for 1 h. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x2). The combined organic extracts were dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-50% acetonitrile/water) afforded the title compound as a beige solid (6.5 mg, 19%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.94 (m, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.70-7.64 (m, 1H), 7.55-7.51 (m, 2H), 7.50-7.45 (m, 4H), 6.37-6.28 (m, 2H), 6.26-6.19 (m, 1H), 3.44-3.40 (m, 2H), 3.23-3.14 (m, 2H), 2.99-2.94 (m, 1H), 2.94-2.94 (m, 1H), 2.82-2.74 (m, 1H), 2.72-2.64 (m, 2H), 2.49-2.44 (m, 2H), 1.09-1.01 (m, 6H). LCMS: [M+H]+=457.45.
A procedure analogous to Example 53, Step 1 using 6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (28 mg, 0.061 mmol) and 2-bromoethanol, 95% (22.68 mg, 0.182 mmol) at 70° C. for 1 h. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x2). The combined organic extracts were dried over Na2SO4 and concentrated onto Celite®. Reverse phase chromatography (C18; 0-30% acetonitrile/water) afforded the title compound as a beige solid (17.5 mg, 60%). 1H NMR (500 MHz, DMSO-d6) δ 8.06-7.99 (m, 1H), 7.98-7.95 (m, 1H), 7.71-7.67 (m, 1H), 7.55-7.49 (m, 4H), 7.39-7.33 (m, 2H), 6.47-6.31 (m, 2H), 4.47-4.37 (m, 1H), 3.60-3.53 (m, 2H), 3.50-3.45 (m, 2H), 3.04-2.93 (m, 4H), 2.85-2.78 (m, 1H), 2.51-2.43 (m, 2H), 2.15-2.02 (m, 2H), 1.92-1.84 (m, 1H), 1.80-1.74 (m, 1H), 1.71-1.62 (m, 1H), 1.54-1.45 (m, 1H); LCMS: [M+H]+=461.46.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (125 mg, 0.326 mmol) and 1-Boc-4-(4-bromobenzenesulfonyl)piperidine (110 mg, 0.272 mmol) [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (19.89 mg, 0.027 mmol), and aqueous K3PO4 (0.418 mL of a 1.3 M solution, 0.544 mmol) afforded the title compound (91 mg, 52%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.92 (d, J=7.8 Hz, 1H), 7.89-7.83 (m, 4H), 7.78 (d, J=10.1 Hz, 1H), 7.48 (dd, J=8.0, 1.7 Hz, 1H), 7.46 (s, 1H), 6.57 (s, 2H), 4.00 (br d, J=10.0 Hz, 2H), 3.50 (tt, J=11.9, 3.5 Hz, 1H), 3.41 (td, J=6.4, 2.6 Hz, 2H), 2.95 (br t, J=6.6 Hz, 2H), 2.72 (br d, J=4.5 Hz, 2H), 1.85 (br d, J=11.0 Hz, 2H), 1.36 (s, 9H), 1.35-1.30 (m, 2H); LCMS: [M−1]−=579.16, [M−100+H]+=481.35.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (105 mg, 0.274 mmol) and 3-[(4-bromobenzene)sulfonyl]azetidine (63.1 mg, 0.228 mmol) [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (16.71 mg, 0.023 mmol), aqueous K3PO4 (0.351 mL of a 1.3 M solution, 0.457 mmol), DME (4 mL) and water (2 mL) to provide the title compound (37 mg, 36%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.90-7.87 (m, 2H), 7.87-7.83 (m, 2H), 7.77 (d, J=10.1 Hz, 1H), 7.48 (dd, J=8.0, 1.5 Hz, 1H), 7.46 (s, 1H), 6.56 (br s, 2H), 4.57-4.49 (m, 1H), 3.77 (dd, J=8.6, 7.0 Hz, 2H), 3.54 (t, J=8.6 Hz, 2H), 3.41 (td, J=6.5, 2.7 Hz, 2H), 3.32 (br s, 1H), 2.95 (br t, J=6.5 Hz, 2H); LCMS: [M+H]+=453.14.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (133 mg, 0.347 mmol) and 1-bromo-4-(ethylsulfonimidoyl)benzene (71.8 mg, 0.289 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (21.16 mg, 0.029 mmol), aqueous K3PO4 (0.445 mL of a 1.3 M solution, 0.578 mmol), DME (4 mL) and water (2 mL) afforded the title compound (57 mg, 46%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=8.1 Hz, 1H), 7.89 (d, J=8.4 Hz, 2H), 7.79 (br d, J=7.9 Hz, 2H), 7.75 (d, J=10.0 Hz, 1H), 7.48 (br d, J=8.1 Hz, 1H), 7.46 (s, 1H), 6.51 (br s, 2H), 4.18 (s, 1H), 3.41 (br dd, J=6.1, 4.0 Hz, 2H), 3.14 (q, J=7.3 Hz, 2H), 2.95 (br t, J=6.4 Hz, 2H), 1.08 (t, J=7.3 Hz, 3H); LCMS: [M+1]+=426.12.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (126 mg, 0.329 mmol) and (4-bromophenyl)(imino)methyl-lambda6-sulfanone (64 mg, 0.274 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (20.05 mg, 0.027 mmol), K3PO4 (0.422 mL of a 1.3 M aqueous solution, 0.548 mmol), DME (4 mL) and water (2 mL) to provide the title compound (59 mg, 50%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br d, J=8.2 Hz, 3H), 7.91 (br d, J=7.9 Hz, 1H), 7.79 (br d, J=7.9 Hz, 2H), 7.74 (br d, J=10.0 Hz, 1H), 7.48 (br d, J=8.1 Hz, 1H), 7.46 (s, 1H), 6.50 (br s, 2H), 4.21 (s, 1H), 3.41 (br s, 2H), 3.32 (s, 2H), 3.08 (s, 3H), 2.96 (br t, J=6.1 Hz, 2H); LCMS: [M+H]+=411.15.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (107 mg, 0.279 mmol), 1-bromo-4-[(trifluoromethyl)sulfonyl]benzene (67.3 mg, 0.233 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (17.02 mg, 0.023 mmol), aqueous K3PO4 (0.358 mL of a 1.3 M solution, 0.465 mmol), DME (4 mL) and water (2 mL) afforded the title compound (27 mg, 24%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 8.15-8.10 (m, 2H), 8.05 (d, J=8.3 Hz, 2H), 7.96 (br s, 1H), 7.92 (d, J=7.9 Hz, 1H), 7.86 (d, J=10.1 Hz, 1H), 7.49 (d, J=8.1 Hz, 1H), 7.46 (s, 1H), 6.72 (br s, 2H), 3.41 (td, J=6.4, 2.4 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H); LCMS: [M+1]+=466.15.
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (114 mg, 0.299 mmol) and 4-(4-bromophenyl)-1,2-dimethylpiperazine (67 mg, 0.249 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.21 mg, 0.025 mmol), K3PO4 (0.383 mL, 0.498 mmol), DME (4 mL) and water (2 mL) to provide the title compound (35 mg, 31%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.46 (dd, J=8.0, 1.5 Hz, 1H), 7.44 (s, 1H), 7.39 (d, J=7.8 Hz, 2H), 6.97 (d, J=8.9 Hz, 2H), 6.17 (s, 2H), 3.60-3.51 (m, 2H), 3.40 (td, J=6.5, 2.7 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.83-2.78 (m, 1H), 2.75 (td, J=11.5, 2.9 Hz, 1H), 2.38 (dd, J=11.6, 10.3 Hz, 1H), 2.26-2.22 (m, 1H), 2.21 (s, 3H), 2.12 (dtt, J=12.7, 6.2, 3.1 Hz, 1H), 1.05 (d, J=6.1 Hz, 3H); LCMS: [M+H]+=445.99.
A procedure analogous to Example 53, Step 1 was employed stirring 6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.086 mmol) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (0.050 mL, 0.346 mmol) and Et3N (0.120 ml, 0.864 mmol) overnight at RT. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x2). The combined organic extracts were dried and concentrated onto Celite®. Silica gel chromatography (eluting with 0-70% EtOAc/Hex) afforded the title compound as a beige solid (37 mg, 78% yield based on purity of 90%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.4-7.5 (m, 4H), 7.3-7.3 (m, 2H), 6.2-6.3 (m, 2H), 3.4-3.4 (m, 2H), 3.2-3.3 (m, 2H), 2.9-3.0 (m, 4H), 2.7-2.8 (m, 1H), 2.3-2.5 (m, 2H), 1.8-1.9 (m, 1H), 1.7-1.8 (m, 1H), 1.6-1.7 (m, 1H), 1.4-1.5 (m, 1H); LCMS: [M+H]+=499.38.
Step 1: 6-(2-amino-6-fluoro-5-(4-(4-(2,2,2-trifluoroethyl)morpholin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one:
A procedure analogous to that of Example 43, Step 5 using 2-(4-bromophenyl)morpholine (175 mg, 0.723 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (346 mg, 0.904 mmol), Cs2CO3 (707 mg, 2.17 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (68.8 mg, 0.094 mmol) afforded the product as a beige solid (259 mg, 78%). LCMS: [M+H]+=419.28.
Step 2: 6-(2-amino-6-fluoro-5-(4-(4-(2,2,2-trifluoroethyl)morpholin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one:
To a slurry of 6-(2-amino-6-fluoro-5-(4-(morpholin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50.mg, 0.119 mmol) in DMF (2 mL) at RT was added 2,2,2-trifluoroethyl trifluoromethanesulfonate (0.052 mL, 0.358 mmol) and Et3N (0.167 mL, 1.20 mmol) and the mixture was stirred at 80° C. for 2.75 h. The rxn mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL), the org phase was separated, aq phase was extracted with CH2Cl2 (2×3 mL), the combined organic layers were dried over Na2SO4 and concentrated onto Celite®. Silica gel chromatography (eluting with 0-70% EtOAc/Hex) afforded the title compound as an off-white solid (30.5 mg, 49%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 2H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 1H), 7.4-7.4 (m, 2H), 6.3-6.4 (m, 2H), 4.5-4.6 (m, 1H), 3.9-4.0 (m, 1H), 3.7-3.8 (m, 1H), 3.4-3.5 (m, 2H), 3.2-3.3 (m, 2H), 2.9-3.0 (m, 3H), 2.85 (br d, J=11.4 Hz, 1H), 2.5-2.6 (m, 1H), 2.3-2.4 (m, 1H); LCMS: [M+H]+=501.38.
Step 1. (3aR,6aS)-5-(4-iodophenyl)hexahydro-1H-furo[3,4-c]pyrrole
A sealed mixture of hexahydro-1 H-furo[3,4-c]pyrrole·HCl (0.564 g, 3.77 mmol) and K3PO4 (1.60 g, 7.54 mmol) in DMF (20 mL) was stirred at RT overnight. The mixture was then degassed with a stream of N2 and then Cul (0.144 g, 0.754 mmol), (R)-(+)-1,1′-bi-2-naphthol (0.216 g, 0.754 mmol) and 1,4-diiodobenzene (1.244 g, 3.77 mmol) were added as solids. Then degassing step was repeated. The mixture was then stirred sealed at 40° C. for 2 d. The reaction mixture was cooled to RT, diluted with EtOAc, filtered through Celite, concentrated under reduced pressure and purified by flash chromatography (50 g SiO2, using CH2Cl2) to afford the product as a yellow solid (659 mg, 50% yield based on purity of 90%). LCMS: [M+H]+=315.99.
Step 2: 6-(2-amino-6-fluoro-5-(4-((3aR,6aS)-tetrahydro-1H-furo[3,4-c]pyrrol-5(3H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 1, General Method A using (3aR,6aS)-5-(4-iodophenyl)hexahydro-1H-furo[3,4-c]pyrrole (73.1 mg, 0.209 mmol, 90%), PdCl2dppf (15.3 mg, 0.021 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.21 mmol), Cs2CO3 (204 mg, 0.626 mmol), H2O (2 mL) and DME (4 mL) afforded the title compound as a white solid (7.0 mg, 7% yield based on purity of 95%). LCMS: [M+H]+=445.16. 1H NMR (500 MHz, CDCl3-CD3OD) δ ppm 7.99 (br d, J=8.0 Hz, 1H), 7.50 (br d, J=9.7 Hz, 1H), 7.38-7.41 (m, 1H), 7.24-7.36 (m, 3H), 6.63 (br d, J=8.6 Hz, 2H), 3.89-4.05 (m, 2H), 3.57-3.72 (m, 2H), 3.51 (br t, J=6.5 Hz, 2H), 3.34-3.45 (m, 2H), 3.18-3.22 (m, 2H), 2.96-3.08 (m, 4H); LCMS: [M+H]+=445.16.
Step 1: (3aS,6aS)-5-(4-iodophenyl)hexahydro-2H-furo[2,3-c]pyrrole
A sealed mixture of 1,4-diiodobenzene (2.0 g, 6.1 mmol), (3aS,6aS)-hexahydro-2H-furo[2,3-c]pyrrole·HCl (0.907 g, 6.06 mmol) and K3PO4 (2.57 g, 12.1 mmol) in DMF (20 mL) was stirred at rt overnight. The mixture was then degassed with a stream of N2 and then Cul (0.231 g, 1.21 mmol) and a (R)-(+)-1,1′-bi-2-naphthol (0.347 g, 1.21 mmol) were added as solids. Then degassing step was repeated. The mixture was then stirred sealed at RT for 30 min and then at 40° C. for 4 d. Later, the reaction mixture was concentrated under reduced pressure and purified by flash chromatography (50 g SiO2, using MeOH in CH2Cl2) to afford the product as a yellow-grey solid (940 mg, 48% yield based on purity of 97%). LCMS: [M+H]+=316.14.
Step 2. 6-(2-amino-6-fluoro-5-(4-((3aS,6aS)-hexahydro-5H-furo[2,3-c]pyrrol-5-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 1, General Method A using (3aS,6aS)-5-(4-iodophenyl)hexahydro-2H-furo[2,3-c]pyrrole (67.8 mg, 0.209 mmol, 97%)PdCl2dppf (15.27 mg, 0.021 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.21 mmol), Cs2CO3 (204 mg, 0.626 mmol), H2O (2 mL) and DME (4 mL) afforded the product as a tan solid (7 mg, 7% yield based on purity of 99%). 1H NMR (500 MHz, CDCl3) δ 8.10 (d, J=8.0 Hz, 1H), 7.50 (d, J=9.7 Hz, 1H), 7.41 (br d, J=8.0 Hz, 1H), 7.34 (br d, J=7.7 Hz, 2H), 7.27 (s, 1H), 6.54-6.65 (m, 2H), 6.01 (br s, 1H), 4.62 (t, J=5.8 Hz, 1H), 4.58 (s, 1H), 3.87-3.94 (m, 1H), 3.74-3.83 (m, 1H), 3.53-3.58 (m, 2H), 3.48-3.53 (m, 1H), 3.34-3.40 (m, 1H), 3.28-3.33 (m, 1H), 3.15-3.22 (m, 1H), 2.93-3.03 (m, 3H), 2.07-2.17 (m, 1H), 1.78-1.86 (m, 1H); LCMS [M+H]+=445.31.
Step 1: 6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3-(4-bromo-phenyl)-piperidine hydrochloride (175 mg, 0.633 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (303 mg, 0.791 mmol), Cs2CO3 (825 mg, 2.53 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (60.2 mg, 0.082 mmol) in DME (10.5 mL) afforded the product (192 mg, 66% yield) as a beige solid. LCMS: [M+H]+=417.46.
Step 2: 6-(2-amino-6-fluoro-5-(4-(1-(2,2,2-trifluoroethyl)piperidin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a slurry of 6-(2-amino-6-fluoro-5-(4-(piperidin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (45.mg, 0.089 mmol) in DMF (2 mL) at RT, was added 2,2,2-trifluoroethyl trifluoromethanesulfonate (0.026 mL, 0.177 mmol) and Et3N (0.123 mL, 0.886 mmol) and the reaction mixture was stirred at RT for 4 h. After 4 h, additional 2,2,2-trifluoroethyl trifluoromethanesulfonate (0.026 mL, 0.177 mmol) was added, and the mixture was heated at 80° C. for 10 h. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL), the organic phase was separated, the aqueous phase was extracted with CH2Cl2 (2×3 mL), the combined layers were dried over Na2SO4 and concentrated onto Celite®. Purification using silica gel chromatography eluting with hexanes containing 0-80 % EtOAc, followed by reverse phase chromatography (5.5 g column, eluting with water containing 0-70% CH3CN) afforded the title compound as an off white solid (9.5 mg, 20%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.71-7.65 (m, 1H), 7.59-7.52 (m, 2H), 7.51-7.48 (m, 1H), 7.48-7.45 (m, 1H), 7.37 (br d, J=8.1 Hz, 2H), 6.40-6.26 (m, 2H), 3.44-3.37 (m, 3H), 3.23-3.17 (m, 1H), 3.09-3.00 (m, 1H), 2.99-2.94 (m, 2H), 2.88-2.78 (m, 1H), 2.49-2.42 (m, 1H), 1.79-1.72 (m, 1H), 1.72-1.64 (m, 2H), 1.64-1.50 (m, 2H), 1.43-1.33 (m, 1H). LCMS: [M+H]+=499.50.
Step 1: tert-butyl (S)-3-(4-bromophenoxy)piperidine-1-carboxylate
To a suspension of NaH, (60% in mineral oil, 2.3 g, 57.5 mmol) in DMF (20 mL) was added (S)-1-Boc-3-hydroxypiperidine (2.4 g, 11.92 mmol) and 1-bromo-4-fluorobenzene (5.24 ml, 47.7 mmol) at RT. The mixture was stirred at 100° C. for overnight and then evaporated to dryness. The residue is taken up in 50 ml of ice-water and extracted with DCM. The organic extracts were dried over MgSO4 and after filtration, the solvents were evaporated to dryness, to give the crude material. The crude material was dry loaded onto Celite® and purified by flash chromatography (0-30%, Hexanes/EtOAc) to afford the product (2.51 g, 59%) as a yellow oil. LCMS: [M-Me+H]+=341.26.
Step 2: tert-butyl (S)-3-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate
To a solution of tert-butyl (S)-3-(4-bromophenoxy)piperidine-1-carboxylate (155 mg, 0.434 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (128 mg, 0.334 mmol) in 1,4-dioxane (3 mL) was added a solution of K3PO4 (213 mg, 1.00 mmol) in water (1 mL) at RT and the reaction mixture was degassed under argon for 15 minutes. Then XPhos Pd G2 (26.3 mg, 0.033 mmol) was added and the reaction mixture was heated at 90° C. for overnight. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-20%, DCM/MeOH) to afford the product (46.9 mg, 26%) as a beige powder. LCMS: [M+H]+=533.34.
Step 3: (S)-6-(2-amino-6-fluoro-5-(4-(piperidin-3-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl (S)-3-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate (46.9 mg, 0.088 mmol) in CH2Cl2 (4 mL) was added TFA (2 mL, 26.1 mmol) at RT and the reaction mixture was stirred for 30 min. The solvents were removed and the crude mixture was dissolved in MeOH and passed through a PoraPak Rxn CX (15cc-2g) cartridge in a catch & elute method. The cartridge was washed with MeOH (20 mL), then the solution of product in MeOH was added onto the cartridge and the cartridge was rinse with MeOH (3×20 mL). The product was released using 20 mL of a solution of ammonia in MeOH (3N) to afford the free base of the crude material which was dry loaded and purified using flash chromatography (0-30%, MeOH/DCM) to afford the title compound (25.8 mg, 64%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.50-7.43 (m, 4H), 7.01 (d, J=8.8 Hz, 2H), 6.23 (s, 2H), 4.45-4.36 (m, 1H), 3.43-3.38 (m, 2H), 3.21-3.13 (m, 2H), 2.99-2.92 (m, 2H), 2.89-2.81 (m, 1H), 2.73-2.60 (m, 2H), 2.07-1.96 (m, 1H), 1.78-1.69 (m, 1H), 1.64-1.46 (m, 2H); LCMS: [M+H]+=433.42.
Step 1: 8-fluoro-6-(4,4, 5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroiso-quinolin-1(2H)-one
To 6-bromo-5-fluoro-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.205 mmol, 1eq), bis(pinacolato)diboron (58.2 mg, 0.229 mmol, 1.1 eq), KOAc (61.3 mg, 0.625 mmol, 3 eq) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (7.62 mg, 10.41 μmol, 0.05 eq) in 1,4-dioxane (2.5 mL) was added under Ar. The mixture was heated in a microwave for 2 h at 100° C. The LCMS showed less than 10% of starting material. The reaction mixture was used in the next step without purification. LCMS: [M+H]+=292.0 Step 2: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A was used heating a sealed, degassed mixture of H2O (1 mL), 5-bromo-6-fluoro-3-iodopyridin-2-amine (200 mg, 0.63 mmol), 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (184 mg, 0.63 mmol), K2CO3 (183 mg, 1.32 mmol), Pd(PPh3)2Cl2 (53 mg, 0.076 mmol) and DMF (9 mL) in a microwave reactor at 80° C. for 1 h. Purification by flash chromatography (eluting with MeOH in CH2Cl2) afforded the title compound as a tan solid (79.0 mg, 34% yield based on purity of 97%). LCMS: [M+H]+=354.19/356.15.
Step 2. 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 43, Step 5 heating a mixture of 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (86 mg, 0.260 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (79 mg, 0.22 mmol), Cs2CO3 (211 mg, 0.649 mmol), H2O (2 mL) DME (4 mL) and PdCl2dppf (15.8 mg, 0.022 mmol) under microwave irradiation at 90° C. for 1 h, afforded the title compound as a pale yellow solid (42.0 mg, 40% yield based on purity of 98%). 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.54-7.66 (m, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.38 (br d, J=8.0 Hz, 2H), 6.96 (d, J=8.9 Hz, 2H), 6.18 (s, 2H), 3.38-3.45 (m, 2H), 3.10-3.20 (m, 4H), 2.92 (br t, J=6.4 Hz, 2H), 2.63-2.71 (m, 1H), 2.54-2.61 (m, 4H), 1.01 (d, J=6.6 Hz, 6H); LCMS: [M+H]+=478.42.
Step 1: (2S,6R)-4-(4-bromophenyl)-1,2,6-trimethylpiperazine
To a 30 mL vial was added 1-bromo-4-iodobenzene (529 mg, 1.87 mmol), cis-1,2,6-trimethylpiperazine (288 mg, 2.24 mmol), tris(dibenzylideneacetone)dipalladium (0) (51.4 mg, 0.056 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (97 mg, 0.168 mmol) and Cs2CO3 (1828 mg, 5.61 mmol). The vial was sealed with a cap and septum and then the reaction vessel was evacuated and backfilled with nitrogen. Toluene (4 mL) was added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction was then concentrated onto Celite® and purified by flash chromatography (SiO2, hexanes-EtOAc) to give the product (272 mg, 51%) as a brown solid. LCMS: [M+H]+=284.80.
Step 2: 6-(2-amino-6-fluoro-5-(4-((3R,5S)-3,4,5-trimethylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (162 mg, 0.424 mmol), cis-4-(4-bromophenyl)-1,2,6-trimethylpiperazine (100 mg, 0.353 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (25.8 mg, 0.035 mmol), aqueous K3PO4 (0.543 mL of a 1.3 M solution, 0.706 mmol), DME (4 mL) and water (2 mL) afforded the title compound (50 mg, 29%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.46 (d, J=8.1 Hz, 1H), 7.44 (s, 1H), 7.39 (br d, J=8.1 Hz, 2H), 6.97 (br d, J=8.8 Hz, 2H), 6.17 (s, 2H), 3.60 (br d, J=11.0 Hz, 2H), 3.40 (td, J=6.4, 2.6 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.41 (br t, J=9.7 Hz, 2H), 2.20 (br s, 5H), 1.08 (br d, J=5.6 Hz, 6H); LCMS: [M+1]+=461.19.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (119 mg, 0.309 mmol), (2S,6S)-4-(4-bromophenyl)-1,2,6-trimethylpiperazine (73 mg, 0.258 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.86 mg, 0.026 mmol), aqueous K3PO4 (0.397 mL of a 1.3 M solution, 0.516 mmol), DME (4 mL) and water (2 mL) afforded the title compound (31 mg, 25%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.57 (d, J=10.1 Hz, 1H), 7.46 (d, J=8.1 Hz, 1H), 7.44 (s, 1H), 7.39 (br d, J=8.1 Hz, 2H), 6.96 (br d, J=8.7 Hz, 2H), 6.17 (s, 2H), 3.40 (td, J=6.5, 2.6 Hz, 2H), 3.22 (br d, J=1.7 Hz, 2H), 2.95 (br t, J=6.5 Hz, 6H), 2.29 (br s, 3H), 1.05 (br s, 6H); LCMS: [M+H]+=461.34.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.297 mmol), 4-N-morpholinylsulfonylphenylboronic acid (97 mg, 0.357 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (21.77 mg, 0.030 mmol), aqueous K3PO4 (0.458 mL of a 1.3 M solution, 0.595 mmol), DME (4 mL) and water (2 mL) afforded the title compound (20 mg, 14%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.86 (d, J=7.9 Hz, 2H), 7.79-7.74 (m, 3H), 7.49 (d, J=7.9 Hz, 1H), 7.47 (s, 1H), 6.55 (s, 2H), 3.67-3.61 (m, 4H), 3.41 (td, J=6.4, 2.6 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.91-2.87 (m, 4H); LCMS: [M+H]+=483.31.
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.297 mmol) and 4-(pyrrolidinylsulfonyl)phenylboronic acid (91 mg, 0.357 mmol) afforded the title compound (112 mg, 77%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.82 (s, 4H), 7.76 (d, J=10.1 Hz, 1H), 7.50-7.47 (m, 1H), 7.46 (s, 1H), 6.53 (s, 2H), 3.41 (td, J=6.4, 2.5 Hz, 2H), 3.16 (br t, J=6.6 Hz, 4H), 2.99-2.91 (m, 2H), 1.70-1.64 (m, 4H); LCMS: [M+H]+=467.28.
A procedure analogous to Example 93, Step 2 using 6-(2-amino-6-fluoro-5-(4-(piperidin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.089 mmol) and bromoethane (0.013 mL, 0.177 mmol) afforded the title compound (23 mg, 56%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.70-7.64 (m, 1H), 7.53-7.46 (m, 4H), 7.40-7.31 (m, 2H), 6.38-6.27 (m, 2H), 3.46-3.40 (m, 2H), 3.15-3.01 (m, 2H), 3.00-2.94 (m, 2H), 2.49-2.39 (m, 1H), 2.09-1.99 (m, 1H), 1.99-1.89 (m, 1H), 1.78-1.62 (m, 3H), 1.60-1.53 (m, 1H), 1.47-1.30 (m, 2H), 0.90-0.85 (m, 3H); LCMS: [M+H]+=445.54
A procedure similar to that of Example 93, Step 2 using 6-(2-amino-6-fluoro-5-(4-(piperidin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.089 mmol) and 2,2-difluoroethyl trifluoromethanesulfonate (0.023 mL, 0.177 mmol) afforded the title compound (26.5 mg, 59%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.01-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.69-7.65 (m, 1H), 7.55-7.51 (m, 2H), 7.51-7.48 (m, 1H), 7.48-7.45 (m, 1H), 7.37 (d, J=8.2 Hz, 2H), 6.32 (s, 2H), 6.15-5.89 (m, 1H), 3.46-3.39 (m, 2H), 3.27-3.22 (m, 1H), 3.20-3.13 (m, 1H), 3.00-2.94 (m, 2H), 2.77-2.64 (m, 1H), 2.42-2.26 (m, 2H), 1.79-1.71 (m, 1H), 1.70-1.63 (m, 2H), 1.63-1.53 (m, 1H), 1.53-1.43 (m, 1H), 1.41-1.30 (m, 1H); LCMS: [M+H]+=481.45.
A procedure analogous to that of Example 43, Step 5 using 2-(4-bromophenyl)morpholine (175 mg, 0.723 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one(346 mg, 0.904 mmol) afforded the product as a beige solid (259 mg, 69% based on purity of 78%). LCMS: [M+H]+=419.28.
Step 2: 6-(2-Amino-5-(4-(4-ethylmorpholin-2-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 90, Step 2 using 6-(2-amino-6-fluoro-5-(4-(morpholin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 78% purity, 0.093 mmol) and bromoethane (0.014 mL, 0.186 mmol) and heating at 80° C. for 3 h afforded the title compound (27.5 mg, 63%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.67-7.63 (m, 1H), 7.55-7.50 (m, 2H), 7.50-7.46 (m, 1H), 7.46-7.44 (m, 1H), 7.42-7.37 (m, 2H), 6.41-6.25 (m, 2H), 4.54-4.44 (m, 1H), 4.00-3.91 (m, 1H), 3.73-3.63 (m, 1H), 3.45-3.39 (m, 2H), 3.00-2.90 (m, 3H), 2.82-2.75 (m, 1H), 2.42-2.32 (m, 2H), 2.12-2.03 (m, 1H), 1.93-1.84 (m, 1H), 1.03 (t, J=7.2 Hz, 3H); LCMS: [M+H]+=447.54.
A procedure similar to Example 90, Step 2 using 6-(2-amino-6-fluoro-5-(4-(morpholin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 78% purity, 0.093 mmol), and 2,2-difluoroethyl trifluoromethanesulfonate (0.037 mL, 0.280 mmol) and heating at 80° C. for 3 h afforded the title compound (21.5 mg, 45%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.68-7.63 (m, 1H), 7.54 (br d, J=7.7 Hz, 2H), 7.50-7.47 (m, 1H), 7.47-7.44 (m, 1H), 7.42-7.36 (m, 2H), 6.42-6.31 (m, 2H), 6.31-6.07 (m, 1H), 4.63-4.44 (m, 1H), 4.02-3.89 (m, 1H), 3.76-3.64 (m, 1H), 3.47-3.39 (m, 2H), 3.03-2.93 (m, 3H), 2.87-2.74 (m, 3H), 2.44-2.36 (m, 1H), 2.19 (t, J=10.8 Hz, 1H). LCMS: [M+H]+=483.46.
A procedure analogous to that of Example 90, Step 2 was used by heating 6-(2-amino-6-fluoro-5-(4-(piperidin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.089 mmol, purity 82%) and 2-iodopropane (0.018 ml, 0.177 mmol) overnight at 80° C. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x2). The combined organic extracts was dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-40% acetonitrile/water) followed by silica gel chromatography (eluting with MeOH/CH2Cl2 0-5%) afforded the title compound (11.5 mg, 27%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.9-9.0 (m, 1H), 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.7-7.8 (m, 3H), 7.62 (br d, J=7.3 Hz, 2H), 7.5-7.5 (m, 2H), 6.4-6.5 (m, 2H), 4.4-4.5 (m, 1H), 3.5-3.6 (m, 1H), 3.4-3.5 (m, 2H), 3.1-3.2 (m, 1H), 2.9-3.1 (m, 3H), 1.9-2.1 (m, 5H), 1.7-1.7 (m, 1H), 1.2-1.2 (m, 3H), 1.14 (br d, J=6.4 Hz, 3H). LCMS: [M+H]+=459.45
A procedure analogous to that of Example 90, Step 2 using 6-(2-amino-6-fluoro-5-(4-(morpholin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 78% purity, 0.093 mmol, from Example 102) and 2-iodopropane (0.028 mL, 0.280 mmol) afforded the title compound (11 mg, 24%) as an off white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.5 (m, 2H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 1H), 7.4-7.4 (m, 2H), 6.2-6.5 (m, 2H), 4.4-4.6 (m, 1H), 3.9-4.1 (m, 1H), 3.6-3.7 (m, 1H), 3.4-3.4 (m, 2H), 2.9-3.0 (m, 2H), 2.8-2.9 (m, 1H), 2.6-2.7 (m, 2H), 2.3-2.4 (m, 1H), 2.1-2.1 (m, 1H), 0.99 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=461.52.
Step 1: (1S,5R)-1-(4-bromophenyl)-3-methyl-3-azabicyclo[3.1.0]hexane
A suspension of stirred (1S,5R)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (200 mg, 0.840 mmol) in anh. THF(10 mL) was treated with an HCHO solution (280 mg, 0.70 mL, 9.3 mmol, aq 37%) at rt. After 1.5 h of stirring at RT, NaBH(OAc)3 (231 mg, 1.09 mmol) was added in one portion while cooling in an ice-H2O bath. The cooling bath was removed after 5 min and stirring was continued at RT for 20 h. Later, excess MeOH was added, the reaction mixture was concentrated under reduced pressure and then filtered through a Waters PoraPak CX column to afford the product as a pale yellow oil (162 mg, 76%). LCMS: [M+H]+=252.14/254.17.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1S,5R)-3-methyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 1, General Method A using (1S,5R)-1-(4-bromophenyl)-3-methyl-3-azabicyclo[3.1.0]hexane (81 mg, 0.321 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (124 mg, 0.324 mmol), Cs2CO3 (314 mg, 0.964 mmol), PdCl2dppf (23.5 mg, 0.032 mmol), DME (3 mL), H2O (1.5 mL) afforded the title compound as an off white solid (35 mg, 24% yield based on purity of 95%). 1H NMR (500 MHz, DMSO-d6) δ 7.93-7.97 (m, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.62 (d, J=10.0 Hz, 1H), 7.43-7.49 (m, 4H), 7.18 (d, J=8.3 Hz, 2), 6.29 (s, 2H), 3.39-3.44 (m, 2H), 3.30-3.32 (m, 1H), 3.24-3.28 (m, 1H), 2.93-3.00 (m, 3H), 2.39 (dd, J=8.6, 3.4 Hz, 1H), 2.30 (s, 3H), 1.77-1.82 (m, 1H), 1.36 (t, J=4.0 Hz, 1H), 0.77 (dd, J=7.8, 3.8 Hz, 1H); LCMS: [M+H]+=429.43.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate
A procedure analogous to Example 43, Step 5 using 4-(N-Boc-piperidino)phenylboronic acid pinacol ester (300 mg, 0.773 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (200 mg, 0.595 mmol) afforded the product as a beige solid (207 mg, 59% yield based on purity of 87%). LCMS: [M+H]+=517.49.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate (205 mg, 0.397 mmol)) at RT with CH2Cl2 (2 mL) and TFA (0.5 mL) was added. The mixture was stirred at RT for 1 h, concentrated, the residue was taken up in CH2Cl2, neutralized with NaHCO3 and adsorbed onto Celite®. Reverse phase chromatography (C18; 0-10% acetonitrile/water) afforded the product as a beige solid (84 mg, 51%); LCMS: [M+H]+=417.46.
Step 3: 6-(2-amino-6-fluoro-5-(4-(1-(2-methoxyethyl)piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 90, Step 2 was used by heating 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (28 mg, 0.067 mmol) and 2-bromoethyl methyl ether (0.013 mL, 0.134 mmol) in solvent at 80° C. for 6 h. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x2). The combined organic extracts was dried and concentrated onto Celite®. Reverse phase chromatography (C18; 0-45% acetonitrile/water) afforded the title compound (20.5 mg, 61%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93-8.00 (m, 1H), 7.91 (d, J=8.0 Hz, 1H), 7.61-7.67 (m, 1H), 7.41-7.53 (m, 4H), 7.25-7.35 (m, 2H), 6.22-6.36 (m, 2H), 3.45-3.50 (m, 2H), 3.40-3.44 (m, 2H), 3.37 (br s, 1H), 3.22-3.29 (m, 3H), 2.98-3.08 (m, 2H), 2.92-2.98 (m, 2H), 2.52-2.59 (m, 2H), 1.95-2.24 (m, 2H), 1.72-1.81 (m, 2H), 1.56-1.71 (m, 2H); LCMS: [M+H]+=475.60.
Step 1: (R)-4-(4-chloro-3-fluorophenyl)-3-methylmorpholine
A 30 mL vial was charged with tris(dibenzylideneacetone)dipalladium (0) (0.16 g, 0.18 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.31 g, 0.54 mmol) and Cs2CO3 (5.8 g, 18 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (14 mL), 4-bromo-1-chloro-2-fluorobenzene (0.83 mL, 7.2 mmol) and (R)-3-methylmorpholine (1.2 mL, 8.6 mmol) were added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 95° C. for 18 h. The reaction mixture was cooled to RT, diluted with CH2Cl2 and filtered through Celite®. The filtrate was concentrated onto Celite® and purified by flash chromatography (1-15% EtOAc/hexanes) to afford the product (1.5 g, 88%). LCMS: [M+H]+=229.9.
Step 2: (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-methylmorpholine
A 30 mL vial was charged with (R)-4-(4-chloro-3-fluorophenyl)-3-methylmorpholine (1.2 g, 5.2 mmol), bis(pinacolato)diboron (1.9 g, 7.8 mmol), KOAc (1.0 g, 10 mmol) and XPhos Pd G2 (0.21 g, 0.26 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (14 mL) was added and the vial was evacuated and backfilled an additional time. The reaction mixture was then heated to 110° C. in an aluminum block for 18 h. The reaction mixture was cooled to RT, diluted with CH2Cl2 and filtered through Celite®. The filtrate was concentrated onto Celite® and purified by flash chromatography (1-15% EtOAc/hexanes) to afford the product (1.5 g, 86%). LCMS: [M+H]+=322.1.
Step 3: (R)-6-(2-amino-6-fluoro-5-(2-fluoro-4-(3-methylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-methylmorpholine (0.11 g, 0.34 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.10 g, 0.30 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.022 g, 0.030 mmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (1 mL) and 2 M aqueous Na2CO3 (0.37 mL, 0.74 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 90° C. for 18 h. The reaction mixture was cooled to RT, diluted with DCM and filtered through Celite®. The filtrate was concentrated onto Celite® and purified by flash chromatography (0.5-9.5% MeOH/DCM+0.5% NH4OH). The product containing fractions were concentrated and further purified by reverse phase chromatography (Biotage SNAP C18; 5-85% MeCN/water+0.1% Formic Acid). Isolation of the title compound was achieved by a catch and release procedure using Biotage SCX2 silica gel to afford the title compound (15 mg, 11%) as an off-white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.4-7.5 (m, 3H), 7.29 (t, J=8.7 Hz, 1H), 6.7-6.8 (m, 2H), 6.27 (s, 2H), 3.9-4.0 (m, 2H), 3.7-3.7 (m, 2H), 3.54 (dt, J=11.5, 3.1 Hz, 1H), 3.41 (dt, J=6.5, 2.6 Hz, 2H), 3.02 (dt, J=12.0, 3.7 Hz, 1H), 2.95 (t, J=6.5 Hz, 2H), 1.05 (d, J=6.5 Hz, 3H); LCMS: [M+H]+=451.4.
A procedure analogous to Example 43, Step 5 using tert-butyl 3-(4-bromophenyl)-2,5-dihydro-1H-pyrrole-1-carboxylate (350 mg, 1.08 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (538 mg, 1.40 mmol) in solvent at 90° C. for 1.25 h. The reaction mixture was partitioned between brine and EtOAc. The layers were separated and the aqueous layer was further extracted with EtOAc (x2). Silica gel chromatography (eluting with 0-100% EtOAc/hexanes) afforded the product (417 mg, 73%) as a beige solid. LCMS: [M+H]+=501.45
Step 2: 6-(2-amino-5-(4-(2,5-dihydro-1H-pyrrol-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (TFA salt)
A solution of tert-butyl 3-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-2,5-dihydro-1H-pyrrole-1-carboxylate (417 mg, 0.791 mmol) at RT with CH2Cl2 (2 mL) was treated with TFA (0.5 mL) and the mixture was stirred for 30 min. The reaction mixture was concentrated onto Celite®. Reverse phase chromatography (C18; 0-20% acetonitrile/water) afforded the trifluoroacetic acid salt of the title compound as a beige solid (347 mg, 81%). 1H NMR (500 MHz, DMSO-d6) δ 9.49-9.31 (m, 2H), 8.02-7.95 (m, 1H), 7.95-7.89 (m, 1H), 7.73-7.67 (m, 1H), 7.66-7.61 (m, 2H), 7.60-7.55 (m, 2H), 7.52-7.47 (m, 1H), 7.46 (s, 1H), 6.51-6.46 (m, 1H), 6.44-6.35 (m, 2H), 4.46-4.35 (m, 2H), 4.24-4.14 (m, 2H), 3.46-3.40 (m, 2H), 3.02-2.94 (m, 2H); LCMS: [M +H]+=401.4.
Step 1: (1R,5S)-1-(4-bromophenyl)-3-isopropyl-3-azabicyclo[3.1.0]hexane
A solution of (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (200 mg, 0.840 mmol) in anh. CH2Cl2 (18 mL) was treated with acetone (0.12 mL, 1.7 mmol) at rt and glacial acetic acid (AcOH) (0.096 mL, 1.68 mmol). After stirring for 1 h at RT, NaBH(OAc)3 (445 mg, 2.10 mmol) was added in one portion at RT and stirring was continued for 2 d. MeOH (2 mL) and satd aq NaHCO3 (6 mL) were added. After separation of the layers, the aqueous phase was extracted with CH2Cl2. The combined organic layers were filtered through a plug of Na2SO4 and concentrated under reduced pressure to afford the product as a yellow solid (237.0 mg, quant). LCMS: [M+H]+=280.21/282.24.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-isopropyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A method similar to Example 1, General Method A using (1R,5S)-1-(4-bromophenyl)-3-isopropyl-3-azabicyclo[3.1.0]hexane (80 mg, 0.29 mmol), PdCl2dppf (29 mg, 0.040 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (115 mg, 0.300 mmol), Cs2CO3 (279 mg, 0.857 mmol), H2O (2 mL) and DME (4 mL) afforded the title compound (37 mg, 27% yield based on purity of 94.4%). 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.62 (d, J=10.2 Hz, 1H), 7.42-7.50 (m, 4H), 7.19 (d, J=8.3 Hz, 2H), 6.29 (s, 2H), 3.41 (td, J=6.5, 2.7 Hz, 2H), 3.34-3.37 (m, 1H), 3.05 (d, J=8.6 Hz, 1H), 2.96 (t, J=6.5 Hz, 2H), 2.54-2.59 (m, 1H), 2.43-2.49 (m, 2H), 1.81 (dt, J=7.8, 3.9 Hz, 1H), 1.31 (t, J=3.9 Hz, 1H), 1.03 (dd, J=13.8, 6.3 Hz, 6H), 0.74 (dd, J=7.9, 3.6 Hz, 1H). LCMS: [M+H]+=457.42.
Step 1: (1S,5R)-1-(4-bromophenyl)-3-isopropyl-3-azabicyclo[3.1.0]hexane
A procedure analogous to Example 110, Step 1 using (1S,5R)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (200 mg, 0.840 mmol), anh. CH2Cl2 (18 mL), acetone (0.12 mL, 1.68 mmol), glacial acetic acid (0.096 mL, 1.68 mmol) and NaBH(OAc)3 (445 mg, 2.100 mmol) afforded the product as a red solid (282.0 mg, quant based on purity of 83%) that was used without further purification in the following step. LCMS: [M+H]+=280.21/282.24.
Step 2: 6-(2-Amino-6-fluoro-5-(4-((1S,5R)-3-isopropyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one·TFA
A procedure similar to Example 1, General Method A using (1S,5R)-1-(4-bromophenyl)-3-isopropyl-3-azabicyclo[3.1.0]hexane (80 mg, 0.24 mmol, 83% purity), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (95 mg, 0.249 mmol), PdCl2dppf (24.3 mg, 0.033 mmol), Cs2CO3 (232 mg, 0.711 mmol), H2O (2 mL) and DME (4 mL); by heating, degassed and sealed, in a microwave reactor at 90° C. for 1 h. The reaction mixture was concentrated purified by flash chromatography (25 g SiO2, using CH2Cl2/MeOH/aq NH4OH (89/10/1 v/v/v) in CH2Cl2) and then by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in (H2O +0.05% TFA)) to afford the title compound as a pale yellow solid (47 mg, 34% yield based on purity of 97.4%). 1H NMR (500 MHz, CD3OD) δ 7.93 (d, J=8.1 Hz, 1H), 7.52 (d, J=9.8 Hz, 1H), 7.43 (d, J=7.3 Hz, 2H), 7.36-7.40 (m, 1H), 7.35 (s, 1H), 7.27 (d, J=8.4 Hz, 2H), 4.00 (d, J=11.3 Hz, 1H), 3.72 (d, J=11.3 Hz, 1H), 3.52-3.61 (m, 2H), 3.34-3.48 (m, 3H), 2.95 (t, J=6.7 Hz, 2H), 2.09-2.19 (m, 1H), 1.32 (t, J=6.5 Hz, 6H), 1.15-1.23 (m, 2H); LCMS: [M+H]+=457.42.
Step 1: (R)-1-(4-chlorophenyl)-3-fluoropyrrolidine
A procedure similar to Example 108, Step 1 using 1-chloro-4-iodobenzene (0.25 g, 1.0 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.029 g, 0.031 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.055 g, 0.094 mmol), Cs2CO3 (1.4 g, 4.2 mmol), toluene (5 mL) and (R)-(−)-3-fluoropyrrolidine hydrochloride (0.16 g, 1.3 mmol) afforded the product (150 mg, 73%). LCMS: [M+H]+=200.3.
Step 2: (R)-3-fluoro-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A procedure analogous to Example 108, Step 3 using (R)-1-(4-chlorophenyl)-3-fluoropyrrolidine (0.15 g, 0.76 mmol), bis(pinacolato)diboron (0.29 g, 1.1 mmol), KOAc (0.15 g, 1.5 mmol) and XPhos Pd G2 (0.045 g, 0.057 mmol) afforded the product (140 mg, 64%). LCMS: [M+H]+=292.0.
Step 3: (R)-6-(2-amino-6-fluoro-5-(4-(3-fluoropyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 108, Step 3 using (R)-3-fluoro-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.075 g, 0.26 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.22 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.016 g, 0.022 mmol), 1,4-dioxane (1 mL) and 2 M aqueous Na2CO3 (0.28 mL, 0.56 mmol) afforded the title compound (1.0 mg, 1.1%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.57 (d, J=10.1 Hz, 1H), 7.4-7.5 (m, 2H), 7.39 (d, J=7.8 Hz, 2H), 6.63 (d, J=8.7 Hz, 2H), 6.13 (s, 2H), 5.4-5.5 (m, 1H), 3.5-3.6 (m, 3H), 3.4-3.4 (m, 5H), 2.96 (t, J=6.5 Hz, 2H); LCMS: [M+H]+=421.4.
Step 1: (S)-1-(4-chlorophenyl)-3-fluoropyrrolidine
A procedure analogous to Example 108, Step 1 using 1-chloro-4-iodobenzene (0.250 g, 1.05 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.029 g, 0.031 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.055 g, 0.094 mmol) and Cs2CO3 (1.366 g, 4.19 mmol) to afford the product (182 mg, 87%). LCMS: [M+H]+=200.18.
Step 2: (S)-3-fluoro-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A procedure analogous to Example 108, Step 2 using (S)-1-(4-chlorophenyl)-3-fluoropyrrolidine (0.182 g, 0.912 mmol), bis(pinacolato)diboron (0.347 g, 1.37 mmol), KOAc (0.179 g, 1.823 mmol) and XPhos Pd G2 (0.054 g, 0.068 mmol afforded the product (168 mg, 63%). LCMS: [M+H]+=292.15.
Step 3: (S)-6-(2-amino-6-fluoro-5-(4-(3-fluoropyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using (S)-3-fluoro-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.075 g, 0.257 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.223 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.016 g, 0.022 mmol), 1,4-dioxane (1 mL) and aqueous Na2CO3 (2M) (0.279 mL, 0.558 mmol) to afford the title compound (2 mg, 2.1%) as an off-white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.49 (d, J=10.1 Hz, 1H), 7.4-7.4 (m, 2H), 7.32 (d, J=7.7 Hz, 2H), 6.55 (d, J=8.8 Hz, 2H), 6.05 (s, 2H), 5.3-5.5 (m, 1H), 3.4-3.6 (m, 2H), 3.3-3.4 (m, 4H), 2.88 (t, J=6.5 Hz, 2H); LCMS: [M+H]+=421.4.
Step 1: (R)-3-methoxy-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A procedure analogous to Example 108, Step 1 using 1-chloro-4-iodobenzene (0.250 g, 1.05 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.029 g, 0.031 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.055 g, 0.094 mmol), Cs2CO3 (1.37 g, 4.19 mmol) and (R)-3-methoxy-pyrrolidine hydrochloride (0.173 g, 1.258 mmol) afforded the product (202 mg, 91%). LCMS: [M+H]+=211.99.
Step 2: (R)-3-methoxy-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A procedure analogous to Example 108, Step 2 using (R)-1-(4-chlorophenyl)-3-methoxypyrrolidine (0.202 g, 0.954 mmol), bis(pinacolato)diboron (0.363 g, 1.431 mmol), KOAc (0.187 g, 1.91 mmol) and XPhos Pd G2 (0.056 g, 0.072 mmol) afforded the product (138 mg, 48%). LCMS: [M+H]+=303.95.
Step 3: (R)-6-(2-amino-6-fluoro-5-(4-(3-methoxypyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 108, Step 3 using (R)-3-methoxy-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.078 g, 0.257 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.223 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.016 g, 0.022 mmol) and aqueous Na2CO3 (0.279 ml of a 2M solution, 0.558 mmol) afforded the title compound (20 mg, 21%) as a off-white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.56 (d, J=10.1 Hz, 1H), 7.4-7.5 (m, 2H), 7.37 (d, J=8.1 Hz,), 6.59 (d, J=8.7 Hz, 2H), 6.11 (s, 2H), 4.10 (br d, J=3.2 Hz, 1H), 3.4-3.5 (m, 4H), 3.2-3.3 (m, 6H), 2.96 (br t, J=6.5 Hz, 2H), 2.0-2.1 (m, 2H); LCMS: [M+H]+=433.3.
Step 1: (S)-1-(4-chlorophenyl)-3-methoxypyrrolidine
A procedure analogous to Example 108, Step 1 using 1-chloro-4-iodobenzene (0.250 g, 1.05 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.029 g, 0.031 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.055 g, 0.094 mmol) and CS2CO3 (1.37 g, 4.19 mmol) and Cs2CO3 (1.37 g, 4.19 mmol) afforded the product (246 mg, quantitative yield). LCMS: [M+H]+=211.91.
Step 2: (S)-3-Methoxy-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A procedure analogous to Example 108, Step 2 using (S)-1-(4-chlorophenyl)-3-methoxypyrrolidine (0.246 g, 1.16 mmol), bis(pinacolato)diboron (0.443 g, 1.74 mmol), KOAc (0.228 g, 2.32 mmol) and XPhos Pd G2 (0.069 g, 0.087 mmol) afforded the product (176 mg, 50%). LCMS: [M+H]+=304.03.
Step 3: (S)-6-(2-amino-6-fluoro-5-(4-(3-methoxypyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 108, Step 3 using (S)-3-methoxy-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.078 g, 0.257 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.223 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.016 g, 0.022 mmol) and aqueous Na2CO3 (0.279 mL of a 2.0 M solution, 0.558 mmol) afforded the title compound (15 mg, 16%) as an off-white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.56 (d, J=10.1 Hz, 1H,), 7.4-7.5 (m, 2H), 7.37 (d, J=7.7 Hz, 2H), 6.59 (d, J=8.8 Hz, 2H), 6.11 (s, 2H), 4.1-4.1 (m, 1H), 3.4-3.5 (m, 4H), 3.2-3.3 (m, 6H), 2.96 (t, J=6.5 Hz, 2H), 2.0-2.1 (m, 2H); LCMS: [M+H]+=433.3.
Step 1: (R)-1-(4-chlorophenyl)-3-(methylsulfonyl)pyrrolidine
A procedure analogous to Example 15, Step 1 using 1-chloro-4-iodobenzene (0.250 g, 1.05 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.029 g, 0.031 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.055 g, 0.094 mmol) and Cs2CO3 (1.03 g, 3.15 mmol) and (R)-3-(methylsulfonyl)pyrrolidine (0.188 g, 1.258 mmol) afforded the product (183 mg, 67%). LCMS: [M+H]+=259.96.
Step 2: (R)-3-(methylsulfonyl)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A procedure analogous to Example 15, Step 2 using (R)-1-(4-chlorophenyl)-3-(methylsulfonyl)pyrrolidine (0.183 g, 0.705 mmol), bis(pinacolato)diboron (0.268 g, 1.057 mmol), KOAc (0.138 g, 1.409 mmol) and XPhos Pd G2 (0.042 g, 0.053 mmol) afforded the product (166 mg, 67%). LCMS: [M+H]+=352.24.
Step 3: (R)-6-(2-amino-6-fluoro-5-(4-(3-(methylsulfonyl)pyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 108, Step 3 using (R)-3-(methylsulfonyl)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.090 g, 0.257 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.223 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.016 g, 0.022 mmol) and aqueous Na2CO3 (0.279 mL of a 2M solution, 0.558 mmol) afforded the title compound (1 mg, 1.0%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.58 (br d, J=10.0 Hz, 1H), 7.4-7.5 (m, 2H), 7.41 (br d, J=8.4 Hz, 2H), 6.68 (br d, J=8.2 Hz, 2H), 6.14 (br s, 2H), 4.1-4.1 (m, 2H), 3.63 (br d, J=7.1 Hz, 4H), 3.07 (s, 3H), 2.9-3.0 (m, 4H); LCMS: [M+H]+=481.4.
Step 1: (S)-1-(4-chlorophenyl)-3-(methylsulfonyl)pyrrolidine
A procedure analogous to Example 15, Step 1 using 1-chloro-4-iodobenzene (0.250 g, 1.05 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.029 g, 0.031 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.055 g, 0.094 mmol), Cs2CO3 (1.03 g, 3.15 mmol) and (S)-3-(methylsulfonyl)pyrrolidine (0.188 g, 1.6 mmol) afforded the product (191 mg, 70%). LCMS: [M+H]+=352.17.
Step 2: (S)-3-(methylsulfonyl)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine
A procedure analogous to Example 15, Step 2 using (S)-1-(4-chlorophenyl)-3-(methylsulfonyl)pyrrolidine (0.191 g, 0.735 mmol), bis(pinacolato)diboron (0.280 g, 1.10 mmol), KOAc (0.144 g, 1.47 mmol) and XPhos Pd G2 (0.043 g, 0.055 mmol) afforded the product (200 mg, 77%). LCMS: [M+H]+=352.17.
Step 3: (S)-6-(2-amino-6-fluoro-5-(4-(3-(methylsulfonyl)pyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 108, Step 3 using (S)-3-(methylsulfonyl)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.090 g, 0.257 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.223 mmol) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.016 g, 0.022 mmol) and aqueous Na2CO3 (0.279 ml of a 2M solution, 0.558 mmol) afforded the title compound (1 mg, 1.0%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.58 (br d, J=10.1 Hz, 1H), 7.4-7.5 (m, 2H), 7.41 (br d, J=8.3 Hz, 2H), 6.68 (br d, J=8.1 Hz, 2H), 6.14 (br s, 2H), 4.1-4.1 (m, 2H), 3.63 (br d, J=7.0 Hz, 3H), 3.07 (s, 3H), 2.96 (br t, J=6.1 Hz, 4H,); LCMS: [M+H]+=481.4.
Step 1: 2-(1-(4-nitrophenyl)hydrazineyl)ethan-1-ol:
To a stirred solution of 1-fluoro-4-nitrobenzene (37.5 mL, 354.6 mmol) in CH3CN (500 mL) was added 2-hydrazineylethan-1-ol (27.1 mL, 459.9 mmol) followed by the addition of K2CO3 (54.2 g, 392.2 mmol) under argon atmosphere and stirred at 85° C. for 16 h before cooled to RT. The reaction mixture was filtered through Celite® and washed with EtOAc (500 mL). The combined filtrates were concentrated under reduce pressure to afford crude product (38 g, 54%) as an orange gummy liquid which was used without further purification for the next step. LCMS: [M+H]+=198.39.
Step 2: 2-(1-(4-nitrophenyl)hydrazineyl)ethan-1-ol hydrobromide:
To a stirred solution of crude 2-(1-(4-nitrophenyl)hydrazineyl)ethan-1-ol (45 g, 228.4 mmol) in EtOAc (450 mL) was added 47% aqueous HBr solution (45 mL) at 0° C. and the mixture was stirred at 0° C. for 2 h. The precipitate was collected by filtration and washed with EtOAc (500 mL) to afford the product (41 g, 65%) as a yellow solid. LCMS: [M+H]+=198.14.
Step 3: 1-(2-bromoethyl)-1-(4-nitrophenyl) hydrazine:
To a stirred solution of 2-(1-(4-nitrophenyl)hydrazineyl)ethan-1-ol hydrobromide (42 g, 155.5 mmol) in dry DME (420 mL) was added PBr3 (19 mL) dropwise at 0° C. under an argon atmosphere and then the reaction mixture was stirred at RT for 30 min and heated to 60° C. for 16 h before cooling to RT. The solvent was evaporated to dryness under reduced pressure to afford a gummy liquid, which was diluted with water (100 mL) and basified with NaHCO3 solution at 0° C. The resulting precipitate was filtered off and washed with water, followed by n-pentane, and dried under vacuum to afford the product (28 g, 71%) as a yellow solid. LCMS: [M+2H]+=260.10.
Step 4: 1-(4-nitrophenyl)-1,4,5,6-tetrahydro-1,2,4-triazine:
To a stirred solution of crude 1-(2-bromoethyl)-1-(4-nitrophenyl) hydrazine (28 g, 108.1 mmol) in anhydrous DME (300 mL), was added ethyl formimidate hydrochloride (11.8 g, 108.1 mmol) under an argon atmosphere, and the mixture was stirred at RT for 20 min. Anhydrous NaOAc (18.6 g, 227 mmol) was added, and the reaction mixture was heated at reflux at 90° C. for 16 h and allowed to cool to RT. The solvent was evaporated to dryness to obtain a gummy liquid. The mixture was acidified with 1N HCl to pH=2 and washed with 60% EtOAc: pet ether (2×500 mL). The aqueous layer was adjusted to pH=8.5 by the addition of aqueous Na2CO3 at 0° C. to give a solid precipitate, which was collected by filtration and washed with n-pentane to afford the product (10.5 g, 46%) as an orange solid. LCMS: [M+H]+=207.21.
Step 5: 4-methyl-1-(4-nitrophenyl)-1,4,5,6-tetrahydro-1,2,4-triazine:
To a stirred solution of 1-(4-nitrophenyl)-1,4,5,6-tetrahydro-1,2,4-triazine (10.9 g, 53 mmol) in DMF (120 mL) was added NaH 60% suspension in mineral oil (3.18 g, 79.5 mmol) under argon atmosphere at 0° C. stirred for 30 min at same temperature and then added methyl iodide (6.9 mL, 111.3 mmol). The mixture was stirred at RT for 4 h, then poured into ice. The resulting precipitate was collected by filtration and washed with cold water followed by n-pentane to afford the product (9.4 g, 81%) as an orange solid. LCMS: [M+H]+=221.14.
Step 6: 4-(4-methyl-5,6-dihydro-1,2,4-triazin-1(4H)-yl)aniline:
To a stirred solution of 4-methyl-1-(4-nitrophenyl)-1,4,5,6-tetrahydro-1,2,4-triazine (2.4 g, 10.9 mmol) in ethanol: methanol (125 mL: 250 mL) was added PtO2 (990 mg, 4.36 mmol) and the mixture was stirred under a H2 balloon pressure for 3 h at RT. The reaction mixture was filtered through Celite® bed and washed with methanol (200 mL). The combined filtrates were evaporated under reduced pressure to give the crude residue, which was triturated with diethyl ether to afford the desired aniline (1.36 g, 68%) as a brown solid. LCMS: [M+H]+=191.10.
Step 7: 1-(4-bromophenyl)-4-methyl-1,4,5,6-tetrahydro-1,2,4-triazine:
To a stirred solution of 4-(4-methyl-5,6-dihydro-1,2,4-triazin-1(4H)-yl)aniline (500 mg, 2.63 mmol) in CH3CN (10 mL) was added Cu(I)Br (752 mg, 5.26 mmol) followed by tert-butyl nitrite (1.56 mL, 13.15 mmol) at 0° C. The reaction mixture was stirred at 0° C. for 2 h, then quenched with water and extracted with EtOAc (2×100 mL). The combined organic layer was dried over Na2SO4 and concentrated under reduced pressure to give crude residue which was purified by Prep HPLC to afford the product (30 mg, 4.5%) as a yellow solid. LCMS: [M+H]+=254.00.
Step 8: 6-(2-amino-6-fluoro-5-(4-(4-methyl-5,6-dihydro-1,2,4-triazin-1(4H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (106 mg, 0.276 mmol), 1-(4-bromophenyl)-4-methyl-1,4,5,6-tetrahydro-1,2,4-triazine (54 mg, 0.212 mmol), Pd(amphos)Cl2 (15.0 mg, 0.021 mmol), K3PO4 (135 mg, 0.637 mmol) in H2O (1 mL) and 1,4-dioxane (8 mL) afforded after chromatographic purification the title compound as a pale yellow solid (8 mg, 8% yield based on purity of 96%). 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br s, 1H), 7.83 (d, J=8.0 Hz, 1H), 7.51 (d, J=10.3 Hz, 1H), 7.35-7.47 (m, 2H), 7.31 (br d, J=7.8 Hz, 2H), 7.08 (d, J=8.9 Hz, 2H), 6.71 (s, 1H), 6.08 (s, 2H), 3.47-3.58 (m, 2H), 3.34 (td, J=6.5, 2.7 Hz, 2H), 3.29 (br t, J=5.0 Hz, 2H), 2.83-2.94 (m, 2H), 2.79 (s, 3H). LCMS: [M+H]+=431.39.
A procedure analogous to Example 90, Step 2 heating 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.072 mmol) and 2-bromoethanol, 95% (27.0 mg, 0.216 mmol) at 80° C. for 1 h afforded, after workup and purification, the title compound as an off white solid (17.5 mg, 50%). 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.67-7.61 (m, 1H), 7.51-7.45 (m, 4H), 7.33-7.28 (m, 2H), 6.32-6.21 (m, 2H), 4.49-4.31 (m, 1H), 3.56-3.50 (m, 2H), 3.44-3.40 (m, 2H), 3.04-2.93 (m, 4H), 2.50-2.47 (m, 1H), 2.46-2.35 (m, 2H), 2.15-2.01 (m, 2H), 1.78-1.73 (m, 2H), 1.72-1.62 (m, 2H). LCMS: [M+H]+=461.46.
A procedure analogous to Example 90, Step 2 was followed by heating 6-(2-amino-5-(4-(2,5-dihydro-1 H-pyrrol-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one TFA salt (45 mg, 0.087 mmol) and bromoethane (0.019 mL, 0.262 mmol) for 4.5 h at RT. Standard workup and purification afforded the title compound as an off-white powder (20.5 mg, 52%). 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.94 (m, 1H), 7.93-7.90 (m, 1H), 7.70-7.65 (m, 1H), 7.57-7.53 (m, 2H), 7.51-7.44 (m, 4H), 6.50-6.28 (m, 3H), 3.85-3.74 (m, 2H), 3.64-3.55 (m, 2H), 3.44-3.40 (m, 2H), 3.01-2.94 (m, 2H), 2.67 (q, J=7.2 Hz, 2H), 1.16-1.06 (m, 3H). LCMS: [M+H]+=429.43.
A procedure analogous to Example 90, Step 2 was used by heating 6-(2-amino-5-(4-(2,5-dihydro-1 H-pyrrol-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one TFA (45 mg, 0.087 mmol) and 2-iodopropane (0.035 mL, 0.350 mmol) overnight at RT. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x3). The combined organic layers were dried over Na2SO4 filtered and concentrated onto Celite®. Reverse phase chromatography (C18; 0-50% acetonitrile/water) followed by silica gel chromatography (eluting with CH2Cl2 containing 0-4% MeOH and 0-0.4% NH4OH) afforded the title compound as an off white solid (17.5 mg, 43%). 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.73-7.64 (m, 1H), 7.58-7.52 (m, 2H), 7.52-7.44 (m, 4H), 6.46-6.27 (m, 3H), 3.86-3.74 (m, 2H), 3.64-3.55 (m, 2H), 3.45-3.39 (m, 2H), 3.01-2.94 (m, 2H), 2.75-2.64 (m, 1H), 1.17-1.00 (m, 6H); LCMS: [M+H]+=443.47.
A procedure analogous to that of Example 90, Step 2 by treating 6-(2-amino-6-fluoro-5-(4-(piperidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.108 mmol, preparation described in Example 93, Step 1) and 2-bromoethyl methyl ether (0.030 mL, 0.324 mmol) overnight at RT. The reaction was worked up and the crude product was purified by reverse phase chromatography (C18; 0-10% acetonitrile/water) to afford the title compound as a beige solid (31 mg, 57%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.66-7.61 (m, 1H), 7.52-7.43 (m, 4H), 7.34-7.29 (m, 2H), 6.37-6.23 (m, 2H), 3.48-3.43 (m, 2H), 3.43-3.40 (m, 2H), 3.26-3.20 (m, 3H), 3.00-2.86 (m, 4H), 2.81-2.71 (m, 1H), 2.66-2.52 (m, 2H), 2.16-1.93 (m, 2H), 1.87-1.79 (m, 1H), 1.75-1.67 (m, 1H), 1.65-1.55 (m, 1H), 1.50-1.39 (m, 1H); LCMS: [M+H]+=475.50.
A procedure analogous to that of Example 90, Step 2 using 6-(2-amino-5-(4-(2,5-dihydro-1H-pyrrol-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one-TFA (45 mg, 0.087 mmol) and 2-bromoethanol, 95% (0.019 mL, 0.262 mmol) and heating overnight afforded, after workup and purification, the title compound as an off-white solid (19 mg, 46%). 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.94 (m, 1H), 7.93-7.87 (m, 1H), 7.70-7.65 (m, 1H), 7.58-7.52 (m, 2H), 7.51-7.41 (m, 4H), 6.50-6.28 (m, 3H), 4.56-4.43 (m, 1H), 3.86-3.77 (m, 2H), 3.67-3.61 (m, 2H), 3.57-3.50 (m, 2H), 3.46-3.39 (m, 2H), 2.96 (br t, J=6.2 Hz, 2H), 2.74 (br t, J=6.1 Hz, 2H); LCMS: [M+H]+=445.41
A procedure analogous to that of Example 90, Step 2 stirring 6-(2-amino-5-(4-(2,5-dihydro-1H-pyrrol-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one-TFA (45 mg, 0.087 mmol) and 2-bromoethyl methyl ether (0.025 mL, 0.262 mmol) for 16 h followed by workup and purification afforded the title compound as an off white solid (25.5 mg, 60%). 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.94 (m, 1H), 7.93-7.87 (m, 1H), 7.70-7.65 (m, 1H), 7.58-7.53 (m, 2H), 7.51-7.43 (m, 4H), 6.40-6.28 (m, 3H), 5.49-5.48 (m, 1H), 3.82 (br s, 2H), 3.63 (br d, J=1.8 Hz, 2H), 3.50-3.45 (m, 2H), 3.41 (dt, J=6.4, 2.4 Hz, 2H), 3.30-3.26 (m, 3H), 2.96 (br t, J=6.4 Hz, 2H), 2.87-2.78 (m, 2H); LCMS: [M+H]+=459.45.
Step 1: 1-((1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexan-3-yl)-2-methylpropan-2-ol
To a MeOH (5 mL) solution of (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (100 mg, 0.420 mmol) was added 1,2-epoxy-2-methylpropane (0.056 mL, 0.63 mmol) at rt. The reaction mixture was stirred at RT for 17 h then concentrated in vacuo to afford the product (130 mg, 85%) was used crude in the following step. LCMS: [M+H]+=310.24/312.27.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-(2-hydroxy-2-methylpropyl)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 1-((1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexan-3-yl)-2-methylpropan-2-ol (65 mg, 0.18 mmol, 85% purity), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (82 mg, 0.21 mmol, PdCl2dppf (13.0 mg, 0.018 mmol), Cs2CO3 (174 mg, 0.534 mmol), DME (4 mL) and H2O (2 mL) afforded the title compound as a beige solid (28.4 mg, 32%); 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.62 (d, J=10.2 Hz, 1H), 7.43-7.55 (m, 4H), 7.18 (d, J=8.3 Hz, 2H), 6.28 (s, 2H), 4.06 (s, 1H), 3.36-3.47 (m, 3H), 3.14 (d, J=8.8 Hz, 1H), 2.96 (br t, J=6.4 Hz, 2H), 2.66 (d, J=8.6 Hz, 1H), 2.54-2.59 (m, 1H), 2.35-2.43 (m, 2H), 1.76 (dt, J=7.7, 3.8 Hz, 1H), 1.37 (t, J=3.9 Hz, 1H), 1.09 (d, J=2.2 Hz, 6H), 0.75 (dd, J=7.8, 3.5 Hz, 1H). LCMS: [M+H]+=487.44.
Step 1: 1-((1S,5R)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexan-3-yl)-2-methylpropan-2-ol
To a MeOH (5 mL) solution of (1S,5R)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (0.060 g, 0.25 mmol) was added 1,2-epoxy-2-methylpropane (0.045 mL, 0.50 mmol) at RT and stirring was continued for 21 h. The reaction mixture was concentrated under reduced pressure to afford the product that was used in its entirety in the following step. LCMS: [M+H]+=310.24/312.27.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1S,5R)-3-(2-hydroxy-2-methylpropyl)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 1-((1S,5R)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexan-3-yl)-2-methylpropan-2-ol (78 mg, 0.251 mmol, entire material from step 1), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.302 mmol), PdCl2dppf (28 mg, 0.038 mmol), Cs2CO3 (246 mg, 0.754 mmol), DME (4.5 mL) and H2O (2 mL) afforded the title compound as a beige solid (50 mg, 41% yield for 2 steps). 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.62 (d, J=10.2 Hz, 1H), 7.42-7.50 (m, 4H), 7.18 (d, J=8.44 Hz, 2H), 6.29 (s, 2H), 4.06 (s, 1H), 3.38-3.45 (m, 3H), 3.14 (d, J=8.8 Hz, 1H), 2.96 (t, J=6.5 Hz, 2H), 2.66 (d, J=8.6 Hz, 1H), 2.54-2.59 (m, 1H), 2.35-2.43 (m, 2H), 1.76 (dt, J=7.7, 3.8 Hz, 1H), 1.37 (t, J=3.9 Hz, 1H), 1.05-1.12 (m, 6H), 0.75 (dd, J=7.8, 3.6 Hz, 1H); LCMS: [M+H]+=487.44.
A procedure analogous to that of Example 90, Step 2, by stirring 6-(2-amino-5-(4-(2,5-dihydro-1 H-pyrrol-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one-TFA (45 mg, 0.087 mmol) and 2,2-difluoroethyl trifluoromethanesulfonate (0.029 ml, 0.219 mmol) for 3 h at RT. Workup and purification afforded the title compound as a white solid (19 mg, 44%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.93 (m, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.70-7.66 (m, 1H), 7.58-7.54 (m, 2H), 7.51-7.43 (m, 4H), 6.44-6.31 (m, 3H), 6.25-5.98 (m, 1H), 3.99-3.91 (m, 2H), 3.80-3.70 (m, 2H), 3.45-3.40 (m, 2H), 3.16-3.07 (m, 2H), 3.00-2.91 (m, 2H); LCMS: [M+H]+=465.47.
In a vial was placed tert-butyl 4-((4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)sulfonyl)piperidine-1-carboxylate (91 mg, 0.157 mmol), CH2Cl2 (5 mL) and TFA (0.012 mL, 0.157 mmol). The flask was mixed by vortexing and then allowed to sit for 2 h, when LCMS indicated complete conversion. The reaction was concentrated and then purified by loading onto a column containing PoraPak RxnCX acidic PE resin, washing (MeOH) and then eluting with 3% NH4OH in MeOH to provide after concentrating solvent the title compound (26 mg, 34%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.96 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.89-7.85 (m, 2H), 7.85-7.82 (m, 2H), 7.78 (d, J=10.1 Hz, 1H), 7.50-7.47 (m, 1H), 7.46 (s, 1H), 6.56 (s, 2H), 3.41 (td, J=6.5, 2.7 Hz, 3H), 3.36 (br d, J=3.7 Hz, 1H), 3.01 (br d, J=12.3 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.49-2.42 (m, 2H), 1.79 (br d, J=11.9 Hz, 2H), 1.41 (qd, J=12.3, 4.0 Hz, 2H); LCMS: [M+H]+=481.12.
Step 1: 3-chloro-6-fluoro-5-(1-(1-methylpiperidin-4-yl)-1H-pyrazol-4-yl)pyridin-2-amine
A sealed and degassed mixture of 5-bromo-3-chloro-6-fluoropyridin-2-amine (150 mg, 0.665 mmol), PdCl2dppf (49 mg, 0.067 mmol), Cs2CO3 (650 mg, 2.0 mmol), 1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1 H-pyrazol-1-yl)piperidine (194 mg, 0.665 mmol), H2O (5 mL) and DME (10 mL) was heated in a microwave reactor at 90° C. for 1.5 h. The reaction mixture was concentrated and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) to afford the product as a brown solid (71 mg, 32% yield based on purity of 93%). LCMS: [M+H]+=310.12.
Step 2: 6-(2-amino-6-fluoro-5-(1-(1-methylpiperidin-4-yl)-1H-pyrazol-4-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using K3PO4 (136 mg, 0.639 mmol), 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (93 mg, 0.32 mmol, preparation described in Example 95, Step 2), 3-chloro-6-fluoro-5-(1-(1-methylpiperidin-4-yl)-1H-pyrazol-4-yl)pyridin-2-amine (71 mg, 0.213 mmol, 93 % purity), XPhos Pd G2 (17 mg, 0.021 mmol), H2O (6 mL) and MeCN (9 mL) afforded the title compound as a pale yellow solid (53 mg, 55% yield based on purity of 97%). 1H NMR (500 MHz, DMSO-d6) δ 8.12 (br s, 1H), 8.09 (s, 1H), 7.82 (d, J=9.9 Hz, 1H), 7.77 (d, J=1.2 Hz, 1H), 7.62 (d, J=10.0 Hz, 1H), 7.40 (d, J=7.0 Hz, 1H), 6.12 (s, 2H), 4.07-4.17 (m, 1H), 3.38-3.45 (m, 2H), 2.93 (br t, J=6.5 Hz, 2H), 2.85 (br d, J=11.4 Hz, 2H), 2.21 (s, 3H), 1.91-2.09 (m, 6H); LCMS [M+H]+=439.44.
To a solution of (S)-4-(4-chloro-2-(trifluoromethyl)phenyl)-2-isopropylmorpholine (37.1 mg, 0.121 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (47.3 mg, 0.123 mmol) in 1,4-dioxane (3 mL) was added a solution of K3PO4 (79 mg, 0.370 mmol) in water (1 ml) at RT and the reaction mixture was degassed under argon for 15 minutes. Then XPhos Pd G2 (9.71 mg, 0.012 mmol) was added and the reaction mixture was heated at 90° C. for overnight. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography [0-10%, DCM/MeOH] to afford the title compound (11.0 mg, 16%) as a white powder. 1H NMR (500 MHz, CD3OD) δ 8.05 (d, J=7.9 Hz, 1H), 7.82-7.75 (m, 2H), 7.66 (d, J=9.9 Hz, 1H), 7.56-7.50 (m, 2H), 7.47 (s, 1H), 3.99-3.93 (m, 1H), 3.77 (dt, J=2.4, 11.0 Hz, 1H), 3.56 (t, J=6.7 Hz, 2H), 3.07 (t, J=6.5 Hz, 2H), 3.04-2.93 (m, 2H), 2.86 (br d, J=11.0 Hz, 1H), 2.67 (t, J=10.5 Hz, 1H), 1.78-1.68 (m, 1H), 1.34-1.28 (m, 1H), 1.00 (d, J=6.7 Hz, 3H), 0.92 (d, J=6.8 Hz, 3H), NH and NH2 peaks not visible; 19F NMR (471 MHz, CD3OD) 5=−61.46 (s, 3F),−77.40 (s, 1F).
Step 1: 6-(2-Amino-6-fluoro-5-(4-(morpholin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 43, Step 5, by coupling 3-(4-bromophenyl)morpholine hydrochloride (200 mg, 0.718 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (344 mg, 0.897 mmol) afforded the product as a beige solid (166 mg, 53%); LCMS: [M+H]+=419.40.
Step 2: 6-(2-amino-5-(4-(4-ethylmorpholin-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 90, Step 2 was used, heating 6-(2-amino-6-fluoro-5-(4-(morpholin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (32 mg, 0.073 mmol) and bromoethane (0.016 ml, 0.220 mmol) at 80° C. for 6 h. The reaction mixture was partitioned between water (3 mL) and CH2Cl2 (3 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x 2). The combined organic extracts was dried and concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-3% MeOH and 0-0.3% NH4OH) followed by reverse phase chromatography (C18; 0-40% acetonitrile/water) afforded the title compound as a beige solid (15 mg, 44%). 1H NMR (500 MHz, DMSO-d6) δ 7.93-7.99 (m, 1H), 7.89-7.93 (m, 1H), 7.51-7.56 (m, 2 H), 7.46-7.51 (m, 2H), 7.37-7.44 (m, 2H), 6.29-6.45 (m, 2H), 3.85-3.90 (m, 1H), 3.59-3.65 (m, 2H), 3.40-3.45 (m, 2H), 3.18-3.30 (m, 2H), 2.93-3.00 (m, 3H), 2.45-2.50 (m, 1H), 2.20-2.30 (m, 1H), 1.95-2.05 (m, 1H), 0.89-0.94 (m, 3H); LCMS: [M+H]+=447.48.
A procedure analogous to that of Example 90, Step 2 heating 6-(2-amino-6-fluoro-5-(4-(morpholin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.072 mmol) and 2-iodopropane (0.029 ml, 0.287 mmol) overnight at 80° C. afforded, after workup and purification, the title compound as an off white solid (10.5 mg, 30%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.66 (d, J=10.1 Hz, 1H), 7.5-7.5 (m, 2H), 7.5-7.5 (m, 1H), 7.45 (s, 1H), 7.4-7.4 (m, 2H), 6.3-6.4 (m, 2H), 3.8-3.9 (m, 1H), 3.5-3.6 (m, 3H), 3.4-3.4 (m, 2H), 3.2-3.3 (m, 1H), 2.9-3.0 (m, 2H), 2.7-2.8 (m, 2H), 2.4-2.5 (m, 1H), 0.9-1.0 (m, 3H), 0.7-0.8 (m, 3H); LCMS: [M+H]+=461.41.
A procedure analogous to that of Example 90, Step 2 using 6-(2-amino-6-fluoro-5-(4-(morpholin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.072 mmol) and 2,2-difluoroethyl trifluoromethanesulfonate (0.038 mL, 0.287 mmol) and heating overnight at 80° C. afforded the title compound as an off white solid (11.5 mg, 32%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.56 (br d, J=7.7 Hz, 2H), 7.5-7.5 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 2H), 6.3-6.4 (m, 2H), 5.9-6.2 (m, 1H), 3.8-3.9 (m, 1H), 3.6-3.7 (m, 2H), 3.5-3.5 (m, 1H), 3.4-3.4 (m, 2H), 3.3-3.3 (m, 2H), 3.0-3.1 (m, 1H), 2.9-3.0 (m, 2H), 2.6-2.8 (m, 1H), 2.5-2.6 (m, 1H); LCMS: [M+H]+=483.52.
A procedure analogous to that of Example 90, Step 2 using 6-(2-amino-6-fluoro-5-(4-(morpholin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.072 mmol) and 2-bromoethyl methyl ether (0.027 ml, 0.287 mmol) and heating for 5 h at 80° C. afforded the title compound (16 mg, 45%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 2H), 7.5-7.5 (m, 1H), 7.47 (s, 1H), 7.4-7.4 (m, 2H), 6.3-6.4 (m, 2H), 3.8-3.9 (m, 1H), 3.6-3.7 (m, 2H), 3.4-3.5 (m, 2H), 3.3-3.4 (m, 3H), 3.2-3.3 (m, 1H), 3.1-3.2 (m, 3H), 3.0-3.1 (m, 1H), 2.9-3.0, (m, 2H), 2.6-2.6 (m, 1H), 2.3-2.4 (m, 1H), 2.1-2.2 (m, 1H); LCMS: [M+H]+=477.44.
A procedure analogous to that of Example 43, Step 5 using (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (60 mg, 0.252 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (126 mg, 0.328 mmol) afforded the title compound (75 mg, 68%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 4H), 7.2-7.3 (m, 2H), 6.2-6.4 (m, 2H), 3.41 (br s, 2H), 3.1-3.2 (m, 1H), 2.9-3.0 (m, 3H), 2.87 (s, 2H), 1.7-1.7 (m, 1H), 1.0-1.0 (m, 1H), 0.8-0.9 (m, 1H), 0.8-0.8 (m, 1H); LCMS: [M+H]+=415.58.
Step 1: (S)-4-(4-chlorophenyl)-2-isopropylmorpholine
The procedure followed was analogous to Example 47, Step 1 using 1-chloro-4-iodobenzene (0.500 g, 2.10 mmol), (S)-2-isopropylmorpholine (0.298 g, 2.31 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.058 g, 0.063 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.109 g, 0.189 mmol) and Cs2CO3 (2.05 g, 6.29 mmol). Workup and purification afforded the product (404 mg, 80%). LCMS: [M+H]+=240.03.
Step 2: (S)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine
A procedure analogous to Example 47, Step 2 using (S)-4-(4-chlorophenyl)-2-isopropylmorpholine (0.404 g, 1.67 mmol), bis(pinacolato)diboron (0.535 g, 2.106 mmol), KOAc (477 mg, 3.37 mmol) and XPhos Pd G2 (0.099 g, 0.126 mmol) afforded the product (1.44 mmol, 85%). LCMS: [M+H]=332.08.
Step 3: (S)-6-(2-Amino-6-chloro-5-(4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 43, Step 5 was used, heating 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol) and (S)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenylmorpholine (42.7 mg, 0.129 mmol) at 90° C. for 1.75 h. The reaction mixture was partitioned between brine and EtOAc. The organic phase was separated and aqueous phase was further extracted with EtOAc (2×3 mL). The combined organic phase was dried and concentrated onto Celite®. Silica gel chromatography (eluting with 0-3% MeOH/DCM), followed by reverse phase chromatography (C18; 0-40% acetonitrile/water), afforded the title compound as a white solid (37.5 mg, 75%). 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.93 (m, 1H), 7.92-7.87 (m, 1H), 7.51-7.43 (m, 2H), 7.39-7.29 (m, 3H), 7.07-6.96 (m, 2H), 6.34-6.13 (m, 2H), 4.02-3.94 (m, 1H), 3.65-3.58 (m, 2H), 3.57-3.51 (m, 1H), 3.44-3.38 (m, 2H), 3.28-3.21 (m, 1H), 2.98-2.91 (m, 2H), 2.74-2.65 (m, 1H), 2.49-2.42 (m, 1H), 1.80-1.71 (m, 1H), 1.00-0.93 (m, 6H); LCMS: [M+H]+=477.50.
Step 1: (R)-4-(4-chloro-3-fluorophenyl)-2-isopropylmorpholine
A 30 mL vial was charged with 4-bromo-1-chloro-2-fluorobenzene (0.15 g, 0.72 mmol), (R)-2-isopropylmorpholine (0.10 g, 0.79 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.020 g, 0.021 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.037 g, 0.064 mmol) and Cs2CO3 (0.70 g, 2.1 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with nitrogen. Toluene (2.5 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated conventionally at 100° C. for 18 h. The reaction mixture was cooled to RT and partitioned between EtOAc and water. The layers were separated and the aqueous layer was extracted with additional EtOAc (x2). The combined organic extracts were dried and concentrated onto Celite®. Flash chromatography (1-15% EtOAc/hexanes) afforded the product (0.18 g, 99%). LCMS: [M+H]+=258.2.
Step 2: (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine
A 30 mL vial was charged with (R)-4-(4-chloro-3-fluorophenyl)-2-isopropylmorpholine (0.67 g, 2.6 mmol), bis(pinacolato)diboron (0.83 g, 3.3 mmol), KOAc (0.51 g, 5.2 mmol) and XPhos Pd G2 (0.15 g, 0.20 mmol). The vial was sealed with a cap and septum and then the reaction vial was evacuated and backfilled with N2. 1,4-Dioxane (8 mL) was added and the reaction vial was evacuated and backfilled with nitrogen an additional time. The reaction was heated at 90° C. for 18 h. After cooling to RT the reaction mixture was concentrated directly onto Celite® and purified by flash chromatography (1-15% EtOAc/hexanes) to afford the product (0.92 g, quantitative yield). LCMS: [M+H]+=350.2.
Step 3: (R)-6-(2-amino-6-chloro-5-(2-fluoro-4-(2-isopropylmorpholino)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 43, Step 5 using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol) and (R)-4-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-isopropylmorpholine (45.1 mg, 0.129 mmol) afforded the title compound (15.5 mg, 30%) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.4-7.5 (m, 2H), 7.3-7.4 (m, 1H), 7.2-7.3 (m, 1H), 6.8-6.9 (m, 2H), 6.3-6.4 (m, 2H), 3.9-4.0 (m, 1H), 3.6-3.7 (m, 3H), 3.4-3.4 (m, 2H), 3.3-3.3 (m, 1H), 3.2-3.3 (m, 1H), 2.9-3.0 (m, 2H), 2.7-2.8 (m, 1H), 1.7-1.8 (m, 1H), 0.9-1.0 (m, 6H); LCMS: [M+H]+=495.49.
A procedure analogous to that of Example 53, Step 1, by stirring 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one TFA (45 mg, 0.085 mmol) and 2-bromoethanol, 95% (0.018 ml, 0.255 mmol) for 16 h at RT afforded the title compound (7.5 mg, 18%) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 2H), 7.4-7.5 (m, 4H), 6.3-6.4 (m, 2H), 6.2-6.2 (m, 1H), 4.4-4.5 (m, 1H), 3.5-3.6 (m, 2H), 3.4-3.4 (m, 2H), 3.3-3.4 (m, 2H), 3.3-3.3 (m, 2H), 3.1-3.2 (m, 2H), 2.9-3.0 (m, 2H), 2.6-2.7 (m, 2H); LCMS: [M+H]+=459.45.
A procedure analogous to that of Example 90, Step 2, by stirring 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one.TFA (45 mg, 0.085 mmol) and 2-bromoethyl methyl ether (0.024 mL, 0.255 mmol) overnight at RT afforded the title compound as a yellow solid (25.5 mg, 60%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.92 (m, 1H), 7.92-7.87 (m, 1H), 7.67-7.63 (m, 1H), 7.55-7.50 (m, 2H), 7.50-7.42 (m, 4H), 6.38-6.26 (m, 2H), 6.23-6.14 (m, 1H), 3.51-3.47 (m, 2H), 3.43-3.39 (m, 2H), 3.26-3.22 (m, 3H), 3.15-3.08 (m, 2H), 2.95 (br t, J=6.4 Hz, 2H), 2.72-2.64 (m, 2H), 2.62-2.55 (m, 2H), 2.49-2.44 (m, 2H); LCMS: [M+H]+=473.49.
A procedure analogous to that of Example 43, Step 5, by stirring 6-(5-(4-((1R,5S)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (60 mg, 0.145 mmol) and 2-bromoethyl methyl ether (0.041 mL, 0.434 mmol) overnight at RT afforded the title compound as an off white solid (38.5 mg, 54%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 4H), 7.1-7.2 (m, 2H), 6.2-6.3 (m, 2H), 3.4-3.5 (m, 5H), 3.24 (s, 3H), 3.0-3.1 (m, 1H), 2.9-3.0 (m, 2H), 2.6-2.7 (m, 2H), 2.6-2.6 (m, 1H), 2.4-2.5 (m, 1H), 1.8-1.8 (m, 1H), 1.3-1.3 (m, 1H), 0.7-0.8 (m, 1H); LCMS: [M+H]+=473.56.
Step 1: 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A degassed mixture of KOAc (248 mg, 2.52 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (266 mg, 0.721 mmol, 96% purity), Pd2dba3 (40 mg, 0.043 mmol), dicyclohexyl[2-(2,4,6-triisopropylphenyl)phenyl]phosphane (34 mg, 0.072 mmol) and B2pin2 (549 mg, 2.16 mmol) in anh. PhMe (10 mL) was heated sealed at 110° C. for 3 h (˜60% conversion by LCMS). The solvent was removed under reduced pressure and the vacuum-dried solid residue that was mixed with B2pin2 (549 mg, 2.16 mmol), PdCl2dppf (53 mg, 0.072 mmol), B2pin2 (549 mg, 2.16 mmol) and 1,4-dioxane (15 mL). The reaction was degassed than heated in an oil bath at 100° C. for 4 h (complete conversion). The resulting crude product was used as the 1,4-dioxane solution in the following step. LCMS: [M+H]+=402.42.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-isopropyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A method similar to Example 1, General Method A, heating a sealed and degassed mixture of (1R,5S)-1-(4-bromophenyl)-3-isopropyl-3-azabicyclo[3.1.0]hexane (70 mg, 0.250 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (120 mg, 6.23 mL, 0.300 mmol, 19.3 mg/mL) 1,4-dioxane (6.2 mL), PdCl2dppf (18 mg, 0.025 mmol), Cs2CO3 (244 mg, 0.749 mmol) and H2O (2.0 mL) in a microwave reactor at 90° C. for 1 h afforded the title compound (43 mg, 36%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.04 (br s, 1H), 7.56 (br d, J=3.3 Hz, 1H), 7.52-7.55 (m, 1H), 7.33-7.41 (m, 3H), 7.13 (br d, J=7.6 Hz, 2H), 6.22 (s, 2H), 3.31-3.37 (m, 3H), 2.92-3.09 (m, 1H), 2.85 (br t, J=6.5 Hz, 2H), 2.45-2.68 (m, 2H), 2.25-2.41 (m, 1H), 1.77 (br s, 1H), 1.24 (br s, 1H), 0.98 (br s, 6H), 0.70 (br s, 1H). LCMS: [M+H]+=475.41.
A method similar to Example 1, General Method A, heating a sealed and degasssed mixture of (1 S,5R)-1-(4-bromophenyl)-3-isopropyl-3-azabicyclo[3.1.0]hexane (84 mg, 0.249 mmol, 83% purity), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (6.21 mL, 0.299 mmol, 19.3 mg/mL), 1,4-dioxane (6.2 mL), PdCl2dppf (18.21 mg, 0.025 mmol), Cs2CO3 (243 mg, 0.746 mmol) and H2O (2.0 mL) in a microwave reactor at 90° C. for 1 h afforded the title compound as an off white solid (49 mg, 41% yield, based on the purity of 99%). 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.63 (br d, J=3.6 Hz, 1H), 7.60-7.62 (m, 1H), 7.38-7.49 (m, 3H), 7.21 (br d, J=8.0 Hz, 2H), 6.30 (s, 2H), 3.41 (td, J=6.4, 2.5 Hz, 3H), 3.02-3.20 (m, 1H), 2.93 (br t, J=6.4 Hz, 2H), 2.55-2.74 (m, 2H), 2.34-2.46 (m, 1H), 1.61-1.94 (m, 1H), 1.31 (br s, 1H), 0.97-1.15 (m, 6H), 0.72-0.83 (m, 1H); LCMS: [M+H]+=475.41.
Step 1: 1-((1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexan-3-yl)-2-methylpropan-1-one
To a stirred solution of (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (92 mg, 0.39 mmol) in anh. CH2Cl2 (20 mL) was added isobutyryl chloride (0.040 mL, 0.39 mmol) dropwise at 0° C. The reaction was stirred with cooling and then allowed slowly to warm up to RT. The stirring was continued at RT for 16 h, then 37% aq NH4OH (˜1 mL) was added at RT. After stirring for 1 h, aq saturated aqueous NaHCO3 was added (4 mL). The aqueous layer was separated and extracted with CH2Cl2. Combined organics were dried (Na2SO4), concentrated and dried in vacuo to afford the product as an orange gum (136 mg, quant) that was used without further purification. LCMS: [M+H]=308.28/310.09.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-isobutyryl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (71 mg, 0.19 mmol), 1-((1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexan-3-yl)-2-methylpropan-1-one (60 mg, 0.19 mmol), PdCl2dppf (13 mg, 0.018 mmol), Cs2CO3 (173 mg, 0.531 mmol), DME (4 mL) and H2O (2 mL) afforded the title compound as a beige solid (19 mg, 22%). 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (br d, J=7.8 Hz, 1H), 7.64 (br d, J=10.0 Hz, 1H), 7.41-7.59 (m, 4H), 7.25-7.36 (m, 2H), 6.31 (br s, 2H), 4.04-4.23 (m, 1H), 3.70-3.86 (m, 2H), 3.49 (br d, J=11.5 Hz, 1H), 3.38-3.44 (m, 2H), 2.96 (br t, J=6.1 Hz, 2H), 2.57-2.77 (m, 1H), 1.89-2.16 (m, 1H), 1.10 (br d, J=5.4 Hz, 1H), 0.97-1.05 (m, 6H), 0.73-0.88 (m, 1H); LCMS: [M+H]+=485.49.
Step 1: tert-butyl (2S,6S)-4-(4-bromophenoxy)-2,6-dimethylpiperidine-1-carboxylate
To a solution of 4-bromophenol (646 mg, 3.74 mmol), tert-butyl (2R,6R)-4-hydroxy-2,6-dimethylpiperidine-1-carboxylate (504 mg, 2.198 mmol) and triphenylphosphine (634 mg, 2.42 mmol) in THF (10 mL) was added diisopropyl azodicarboxylate (0.519 ml, 2.64 mmol) slowly. The mixture was allowed to stir in a sealed flask at 50° C. for 3 d. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-20%, hexanes/EtOAc) to afford the a mixture of the desired product (444.4 mg, 53%) and 4-bromophenol with a ratio of 1:1 and as a slightly yellow oil which was used for the next step. LCMS: [M-tBu+H]+=328.32.
Step 2: tert-butyl (2S,6S)-4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)-2,6-dimethylpiperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (143 mg, 0.373 mmol) and the mixture of tert-butyl (2S,6S)-4-(4-bromophenoxy)-2,6-dimethylpiperidine-1-carboxylate (186 mg, 0.485 mmol) and 4-bromophenol with a ratio of 1:1 in 1,4-dioxane (3 ml) and K3PO4 (238 mg, 1.12 mmol) in water (1 mL) and XPhos Pd G2 (29.4 mg, 0.037 mmol) afforded the product (60.4 mg, 29%) as a beige powder which was used for the next step. LCMS: [M+H]+=561.40.
Step 3: rac-6-(2-amino-5-(4-(((2R,6R)-2,6-dimethylpiperidin-4-yl)oxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl (2S,6S)-4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)-2,6-dimethylpiperidine-1-carboxylate (60.4 mg, 0.108 mmol) in CH2Cl2 (4 mL) was added TFA (2 mL, 26.1 mmol) at RT and the reaction mixture was stirred for 30 min. The solvents were removed and the crude mixture was dissolved in MeOH and passed through a PoraPak Rxn CX (15cc-2g) cartridge. The cartridge was rinsed with MeOH, and the product was released using 20 mL of a solution of ammonia in MeOH (3N) to afford after removing solvent the title compound (46.5 mg, 89%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.41 (m, 4H), 6.98 (d, J=8.8 Hz, 2H), 6.22 (s, 2H), 4.65 (br s, 1H), 3.43-3.38 (m, 2H), 3.11-3.02 (m, 1H), 2.95 (br t, J=6.4 Hz, 2H), 2.04-1.98 (m, 1H), 1.80 (br s, 1H), 1.60-1.52 (m, 1H), 1.17 (d, J=7.0 Hz, 3H), 1.14-1.10 (m, 1H), 1.04 (d, J=6.4 Hz, 3H), 2H under the water peak; LCMS: [M+H]+=461.49.
Step 1: tert-butyl 4-((7-bromobenzo[d][1,3]dioxol-4-yl)oxy)piperidine-1-carboxylate
To a solution of 7-bromobenzo[d][1,3]dioxol-4-ol (379.7 mg, 1.75 mmol), tert-butyl 4-hydroxy-1-piperidinecarboxylate (352 mg, 1.75 mmol) and triphenylphosphine (459 mg, 1.75 mmol) in anhydrous THF (10 mL) was added diisopropyl azodicarboxylate (0.344 mL, 1.75 mmol) slowly. The mixture was allowed to stir in a sealed flask at 50° C. for overnight. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-30%, hexanes/EtOAc) to afford the product (374.9 mg, 51%) as a colorless oil which solidified overtime. LCMS: [M-tBu+H]+=344.12.
Step 2: tert-butyl 4-((7-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)benzo[d][1,3]dioxol-4-yl)oxy)piperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 4-((7-bromobenzo[d][1,3]dioxol-4-yl)oxy)piperidine-1-carboxylate (177 mg, 0.441 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (130 mg, 0.339 mmol), 1,4-dioxane (3 ml), K3PO4 (216 mg, 1.018 mmol), water (1 mL) and XPhos Pd G2 (26.7 mg, 0.034 mmol) afforded the product (98 mg, 50%) as a beige powder which was used for the next step. LCMS: [M+H]+=577.50.
Step 3: 6-(2-amino-6-fluoro-5-(7-(piperidin-4-yloxy)benzo[d][1,3]dioxol-4-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-((7-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)benzo[d][1,3]dioxol-4-yl)oxy)piperidine-1-carboxylate (97.9 mg, 0.170 mmol) and TFA (2 mL, 26.1 mmol) in CH2Cl2 (4 mL) at RT afforded the title compound (73.4 mg, 86%) as a slightly yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.58 (d, J=9.7 Hz, 1H), 7.40 (s, 2H), 6.88 (d, J=8.1 Hz, 1H), 6.70 (d, J=8.9 Hz, 1H), 6.29 (s, 2H), 5.99 (s, 2H), 4.48-4.40 (m, 1H), 3.44-3.37 (m, 2H), 3.00-2.91 (m, 5H), 2.59-2.52 (m, 2H), 1.95-1.86 (m, 2H), 1.53-1.42 (m, 2H); LCMS: [M+H]+=477.44.
Step 1: tert-butyl 3-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)azetidine-1-carboxylate
A procedure analogous to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (133 mg, 0.347 mmol) and tert-butyl 3-(4-bromophenoxy)azetidine-1-carboxylate (137 mg, 0.416 mmol), 1,4-dioxane (3 ml), K3PO4 (221 mg, 1.04 mmol), water (1 mL) and XPhos Pd G2 (27.3 mg, 0.035 mmol) afforded the product (43 mg, 25%) as a beige powder which was used for the next step. LCMS: [M+H]+=505.50.
Step 2: 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 3-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)azetidine-1-carboxylate (43.1 mg, 0.085 mmol), TFA (2 mL, 26.1 mmol) in CH2Cl2 (2 mL, 26.1 mmol) afforded the title compound (29.2 mg, 80%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.43 (m, 4H), 6.85 (br d, J=8.7 Hz, 2H), 6.24 (s, 2H), 5.04-4.96 (m, 1H), 3.87-3.80 (m, 2H), 3.59-3.53 (m, 2H), 3.43-3.39 (m, 2H), 2.97-2.93 (m, 2H); LCMS: [M+H]+=405.43.
Step 1: tert-butyl 4-(4-bromophenoxy)-2,2-dimethylpiperidine-1-carboxylate
To a stirred solution of 4-bromophenol (264 mg, 1.526 mmol), tert-butyl 4-hydroxy-2,2-dimethylpiperidine-1-carboxylate (350 mg, 1.53 mmol) and triphenylphosphine (400 mg, 1.53 mmol) in anhydrous THF (10 mL) was added diisopropyl azodicarboxylate (0.301 mL, 1.53 mmol) slowly and at RT. The mixture was allowed to stir in a sealed flask at 50° C. for 3 d, and dry loaded onto Celite® and purified by flash chromatography (0-30%, Hexanes/EtOAc) to afford the product (317.6 mg, 54%) as a white powder which was used for the next step. LCMS: [M-tBu+H]+=328.10.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)-2,2-dimethylpiperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 4-(4-bromophenoxy)-2,2-dimethylpiperidine-1-carboxylate (130 mg, 0.339 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (130 mg, 0.339 mmol), 1,4-dioxane (3 mL), K3PO4 (216 mg, 1.02 mmol) in water (1 mL) and XPhos Pd G2 (26.7 mg, 0.034 mmol) afforded the product (53.7 mg, 28%) as a beige powder which was used for the next step. LCMS: [M+H]+=561.48.
Step 3: 6-(2-amino-5-(4-((2,2-dimethylpiperidin-4-yl)oxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)-2,2-dimethylpiperidine-1-carboxylate (53.7 mg, 0.096 mmol), TFA (2 mL, 26.1 mmol) in CH2Cl2 (4 mL) afforded the title compound (37.1 mg, 80%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.96-7.92 (m, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 4H), 6.97 (d, J=8.8 Hz, 2H), 6.22 (s, 2H), 4.61-4.53 (m, 1H), 3.43-3.38 (m, 2H), 2.98-2.92 (m, 2H), 2.89-2.74 (m, 2H), 2.00-1.93 (m, 1H), 1.89-1.82 (m, 1H), 1.37-1.27 (m, 1H), 1.24 (br t, J=11.3 Hz, 1H), 1.10 (s, 3H), 1.08 (s, 3H); LCMS: [M+H]+=461.49.
Step 1: tert-butyl 4-(4-bromo-2,3-difluorophenoxy)piperidine-1-carboxylate
A procedure analogous to Example 147, Step 1 using 4-bromo-2,3-difluorophenol (523.8 mg, 2.51 mmol), tert-butyl 4-hydroxy-1-piperidinecarboxylate (504 mg, 2.51 mmol), triphenylphosphine (657 mg, 2.51 mmol), THF (10 mL), diisopropyl azodicarboxylate (0.493 mL, 2.51 mmol) afforded the product (730.9 mg, 46%) and 4-bromo-2,3-difluorophenol with a ratio of 62:38 and as a colorless oil which was used for the next step. LCMS: [M-Me+H]+=377.21.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2,3-difluorophenoxy)piperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 4-(4-bromo-2,3-difluorophenoxy)piperidine-1-carboxylate (173 mg, 0.441 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (130 mg, 0.339 mmol), 1,4-dioxane (3 mL), K3PO4 (216 mg, 1.02 mmol) in water (1 mL) and XPhos Pd G2 (26.7 mg, 0.034 mmol) afforded the product (83.3 mg, 43%) as a white powder. LCMS: [M+H]+=569.45.
Step 3: 6-(2-amino-5-(2,3-difluoro-4-(piperidin-4-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2,3-difluorophenoxy)piperidine-1-carboxylate (83.3 mg, 0.147 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (56.5 mg, 78%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.53 (d, J=9.5 Hz, 1H), 7.47-7.41 (m, 2H), 7.20 (s, 1H), 7.14 (br d, J=7.8 Hz, 1H), 6.42 (s, 2H), 4.56-4.48 (m, 1H), 3.43-3.37 (m, 2H), 2.94 (s, 4H), 2.61-2.53 (m, 2H), 1.97-1.90 (m, 2H), 1.54-1.46 (m, 2H); LCMS: [M+H]+=469.46.
Step 1: tert-butyl 4-(4-bromo-2,5-difluorophenoxy)piperidine-1-carboxylate
A procedure analogous to Example 147, Step 1 using 4-bromo-2,5-difluorophenol (556 mg, 2.66 mmol), tert-butyl 4-hydroxy-1-piperidinecarboxylate (535.7 mg, 2.66 mmol), triphenylphosphine (698 mg, 2.66 mmol), THF (10 mL) and diisopropyl azodicarboxylate (0.524 ml, 2.66 mmol) afforded the product (867.1 mg, 58%) and 4-bromo-2,5-difluorophenol with a ratio of 70:30 and as a colorless oil which was used for the next step. LCMS: [M-Me+H]+=377.21.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2,5-difluorophenoxy)piperidine-1-carboxylate
A procedure analogous to Example 43, Step 5 using tert-butyl 4-(4-bromo-2,5-difluorophenoxy)piperidine-1-carboxylate (174 mg, 0.444 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (131 mg, 0.342 mmol), 1,4-dioxane (3 ml), K3PO4 (218 mg, 1.03 mmol) in water (1 mL) and XPhos Pd G2 (26.9 mg, 0.034 mmol) afforded the product (85.3 mg, 44%) as a white powder. LCMS: [M+H]+=569.45.
Step 3: 6-(2-amino-5-(2,5-difluoro-4-(piperidin-4-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2,5-difluorophenoxy)piperidine-1-carboxylate (85.3 mg, 0.150 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (60.3 mg, 82%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.53 (d, J=9.7 Hz, 1H), 7.48-7.34 (m, 3H), 7.27 (dd, J=11.6, 7.2 Hz, 1H), 6.39 (s, 2H), 4.55-4.47 (m, 1H), 3.43-3.38 (m, 2H), 2.95 (br d, J=7.1 Hz, 4H), 2.61-2.54 (m, 2H), 1.97-1.89 (m, 2H), 1.53-1.42 (m, 2H); LCMS: [M+H]+=477.44.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3-methoxyphenoxy)piperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (131 mg, 0.342 mmol), tert-butyl 4-(4-bromo-3-methoxyphenoxy)piperidine-1-carboxylate (158 mg, 0.410 mmol), 1,4-dioxane (3 mL) K3PO4 (218 mg, 1.03 mmol) in water (1 mL) and XPhos Pd G2 (26.9 mg, 0.034 mmol) afforded the product (73.0 mg, 38.0%) as a beige powder. LCMS: [M+H]+ 563.43.
Step 2: 6-(2-amino-6-fluoro-5-(2-methoxy-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3-methoxyphenoxy)piperidine-1-carboxylate (73.0 mg, 0.130 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (53.0 mg, 84%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.92 (br s, 1H), 7.89 (d, J=7.9 Hz, 1H), 7.46-7.34 (m, 3H), 7.13 (d, J=8.2 Hz, 1H), 6.63-6.57 (m, 2H), 6.13 (s, 2H), 4.49-4.41 (m, 1H), 3.73 (s, 3H), 3.42-3.37 (m, 2H), 2.94 (br s, 4H), 2.63-2.56 (m, 2H), 1.97-1.89 (m, 2H), 1.51-1.42 (m, 2H); LCMS: [M+H]+=463.44.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-methoxyphenoxy)piperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using 4-(1-Boc-piperidin-4-yloxy)-3-methoxyphenylboronic acid, pinacol ester (79 mg, 0.183 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (55.8 mg, 0.166 mmol), 1,4-Dioxane (3 ml), Cs2CO3 (162 mg, 0.498 mmol) in water (1 mL) and Pd(dppf)Cl2 (18.22 mg, 0.025 mmol) afforded the product (88.7 mg, 95%) as a light brown powder. LCMS: [M+H]+=563.51.
Step 2: 6-(2-amino-6-fluoro-5-(3-methoxy-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-methoxyphenoxy)piperidine-1-carboxylate (88.7 mg, 0.158 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (63.6 mg, 83%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.64 (d, J=10.1 Hz, 1H), 7.48-7.42 (m, 2H), 7.10 (s, 1H), 7.02 (s, 2H), 6.23 (s, 2H), 4.35-4.28 (m, 1H), 3.79 (s, 3H), 3.43-3.37 (m, 2H), 2.95 (br s, 4H), 2.59-2.52 (m, 2H), 1.92-1.85 (m, 2H), 1.51-1.41 (m, 2H); LCMS: [M+H]+=463.44.
To a mixture of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (27 mg, 0.065 mmol), oxetan-3-one (3.99 μl, 0.068 mmol) and AcOH (0.015 mL, 0.259 mmol) in DCE (4 mL), was added NaBH(OAc)3 (41.2 mg, 0.194 mmol) and the slurry obtained was stirred overnight at RT. The reaction mixture was quenched with saturated aqueous NaHCO3 solution. The layers were separated and the aqueous layer was further extracted with DCM (x3). The combined extract was dried and concentrated onto Celite®. Silica gel chromatography (eluting with DCM containing 0-4% MeOH and 0-0.4% NH40H), followed by reverse phase column (C18, 0-30% acetonitrile/water), and then passing through a Waters PoraPak CX cartridge afforded the title compound as an off white powder (8.5 mg, 25% based on purity of 90%). 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.93 (m, 1H), 7.92-7.88 (m, 1H), 7.66-7.61 (m, 1H), 7.51-7.45 (m, 4H), 7.30 (d, J=8.2 Hz, 2H), 6.38-6.20 (m, 2H), 4.58-4.53 (m, 2H), 4.48-4.43 (m, 2H), 3.44-3.39 (m, 5H), 2.99-2.92 (m, 2H), 2.86-2.77 (m, 2H), 2.58-2.53 (m, 1H), 1.91-1.85 (m, 2H), 1.80-1.75 (m, 2H), 1.73-1.66 (m, 2H); LCMS: [M+H]+=473.62.
A procedure analogous to Example 152 using 6-(2-amino-6-fluoro-5-(4-(piperidin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.058 mmol) with oxetan-3-one (3.54 μl, 0.061 mmol). Silica gel chromatography (eluting with DCM containing 0-3% MeOH and 0-0.3% NH4OH), followed by reverse phase column (C18, 0-30% acetonitrile/water afforded the title compound (4 mg, 14%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.68-7.63 (m, 1H), 7.52-7.46 (m, 4H), 7.39-7.33 (m, 2H), 6.38-6.26 (m, 2H), 4.53-4.45 (m, 1H), 4.33-4.26 (m, 1H), 3.80-3.73 (m, 1H), 3.57-3.51 (m, 1H), 3.45-3.39 (m, 4H), 2.99-2.93 (m, 2H), 2.93-2.88 (m, 1H), 2.84-2.78 (m, 1H), 1.96-1.87 (m, 1H), 1.81-1.74 (m, 1H), 1.72-1.64 (m, 2H), 1.63-1.54 (m, 2H); LCMS: [M+H]+=473.62.
A procedure analogous to Example 152 using 6-(2-amino-6-fluoro-5-(4-(morpholin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (37 mg, 0.088 mmol) with oxetan-3-one (5.44 μl, 0.093 mmol)) afforded the title compound (6.5 mg, 14%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.93 (m, 1H), 7.93-7.86 (m, 1H), 7.66 (br d, J=10.0 Hz, 1H), 7.58-7.50 (m, 2H), 7.50-7.47 (m, 1H), 7.47-7.44 (m, 1H), 7.42-7.35 (m, 2H), 6.47-6.27 (m, 2H), 4.64-4.54 (m, 1H), 4.38-4.29 (m, 1H), 3.94-3.84 (m, 2H), 3.70-3.57 (m, 4H), 3.53-3.48 (m, 1H), 3.41 (br s, 2H), 3.24-3.17 (m, 1H), 2.96 (br t, J=6.1 Hz, 2H), 2.81-2.74 (m, 1H), 2.30-2.18 (m, 1H); LCMS: [M+H]+=475.56.
A procedure analogous to Example 152, by the reductive amination of 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one TFA (35 mg, 0.066 mmol) with oxetan-3-one (4.07 μL, 0.070 mmol), afforded the title compound (9.5 mg, 30%) as a yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.72-7.64 (m, 1H), 7.57-7.51 (m, 2H), 7.51-7.40 (m, 4H), 6.41-6.27 (m, 2H), 6.26-6.17 (m, 1H), 4.64-4.56 (m, 2H), 4.56-4.47 (m, 2H), 3.61-3.53 (m, 1H), 3.45-3.40 (m, 2H), 3.37-3.34 (m, 4H), 3.04-2.99 (m, 2H), 2.98-2.93 (m, 2H); LCMS: [M+H]+=471.55.
Step 1: (1R,5S)-1-(4-bromophenyl)-3-(oxetan-3-yl)-3-azabicyclo[3.1.0]hexane
A stirred anhydrous CH2Cl2 (15 mL) solution of (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (220 mg, 0.924 mmol) was treated with glacial acetic acid (0.11 mL, 1.8 mmol) and oxetan-3-one (0.11 mL, 1.8 mmol) at RT. After 1.25 h of stirring at RT, NaBH(OAc)3 (490 mg, 2.31 mmol) was added in one portion and stirring was continued at RT for 2 d. Later, MeOH (2 mL) and satd aq NaHCO3 (6 mL) were added. The separated aqueous layer was extracted with CH2Cl2 and combined organics were filtered through a plug of Na2SO4 and concentrated under reduced pressure. The dried in vacuo product (an orange gum, 267 mg, quant) was used without further purification in the following step. LCMS: [M+H]+=294.21/296.24.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-(oxetan-3-yl)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using (1R,5S)-1-(4-bromophenyl)-3-(oxetan-3-yl)-3-azabicyclo[3.1.0]hexane (70 mg, 0.24 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (96 mg, 0.25 mmol), PdCl2dppf (17 mg, 0.024 mmol), Cs2CO3 (233 mg, 0.714 mmol), H2O (2.0 mL) and DME (4 mL) afforded the title compound as a white solid (5.0 mg, 5%). 1H NMR (500 MHz, CDCl3) δ 8.11 (d, J=8.0 Hz, 1H), 7.51 (d, J=9.5 Hz, 1H), 7.31-7.42 (m, 3H), 7.26 (s, 1H), 7.12 (d, J=8.3 Hz, 2H), 6.03 (br s, 1H), 4.67 (s, 2H), 4.54-4.65 (m, 4H), 3.76 (quin, J=6.4 Hz, 1H), 3.55 (td, J=6.5, 2.8 Hz, 2H), 3.25 (d, J=8.2 Hz, 1H), 2.95-3.04 (m, 3H), 2.59 (d, J=8.2 Hz, 1H), 2.48 (dd, J=8.3, 3.6 Hz, 1H), 1.71 (dt, J=7.9, 4.0 Hz, 1H), 1.45 (t, J=4.3 Hz, 1H), 0.73-0.86 (m, 1H); LCMS: [M+H]+=471.49.
Step 1: 1-(4-chloro-3-fluorophenyl)-N,N-dimethylpyrrolidin-3-amine
A procedure analogous to Example 108, Step 1 using 3-(dimethylamino)pyrrolidine (0.234 mL, 1.43 mmol), 4-bromo-1-chloro-2-fluorobenzene (0.139 mL, 1.19 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.033 g, 0.036 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.062 g, 0.107 mmol) and Cs2CO3 (1.17 g, 3.58 mmol) afforded the product (286 mg, 99%). LCMS: [M+H]+=243.19
Step 2: 1-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N,N-dimethylpyrrolidin-3-amine
A procedure analogous to that described in Example 108, Step 2 using 1-(4-chloro-3-fluorophenyl)-N,N-dimethylpyrrolidin-3-amine (0.286 g, 1.18 mmol), bis(pinacolato)diboron (0.374 g, 1.47 mmol), KOAc (0.231 g, 2.36 mmol) and XPhos Pd G2 (0.070 g, 0.088 mmol) afforded the product (234 mg, 42%) that was judged to be approximately 70% pure and was used without further purification in the next step. LCMS: [M +H]+=335.24.
Step 3: 6-(2-amino-5-(4-(3-(dimethylamino)pyrrolidin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.196 mmol), 1-(4-chlorophenyl)-N,N-dimethylpyrrolidin-3-amine (0.046 g, 0.205 mmol), XPhos Pd G2 (0.015 g, 0.020 mmol) and aqueous K3PO4 (0.376 mL of a 1.3 M solution, 0.489 mmol) afforded the title compound (31 mg, 36%) as a light grey powder. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.56 (d, J=10.1 Hz, 1H), 7.4-7.5 (m, 2H), 7.36 (d, J=7.8 Hz, 2H,), 6.59 (d, J=8.8 Hz, 2H), 6.11 (s, 2H), 3.46 (dd, J=8.9, 7.5 Hz, 1H), 3.4-3.4 (m, 3H), 3.31 (s, 2H), 3.25 (dt, J=9.5, 7.0 Hz, 1H), 3.05 (t, J=8.6 Hz, 1H), 2.96 (t, J=6.5 Hz, 2H), 2.8-2.8 (m, 1H), 2.21 (s, 6H), 2.1-2.2 (m, 1H), 1.8-1.9 (m, 1H); LCMS: [M+H]+=446.4.
Step 1: tert-butyl 3-(N-methylacetamido)pyrrolidine-1-carboxylate
Acetyl chloride (0.11 mL, 1.5 mmol) was added dropwise to a vial containing a solution of 1-boc-3-methylaminopyrrolidine (0.25 g, 1.2 mmol) and triethylamine (0.21 mL, 1.5 mmol) in DCM (5 mL) at RT. The reaction was allowed to stir at RT for 18 h. The reaction mixture was concentrated onto Celite®. Purification by flash chromatography (0.5-9.5% DCM/MeOH+0.5% NH4OH) afforded the product (0.32 g, quantitative yield) as a clear oil. LCMS: [M+H]+=243.3.
Step 2: tert-butyl 3-(ethyl(methyl)amino)pyrrolidine-1-carboxylate
A 2 M solution of borane dimethyl sulfide complex in THF (2.0 mL, 4.0 mmol) was added dropwise to a solution of tert-butyl 3-(N-methylacetamido)pyrrolidine-1-carboxylate (0.32 g, 1.3 mmol) in THF (6 mL) at 0° C. The ice bath was removed and the reaction mixture was heated to 60° C. for 4 h. After cooling to RT the reaction was carefully quenched with the dropwise addition of saturated NaHCO3 (Aq.). Isolation of the desired product was achieved by a catch and release procedure using Biotage SCX2 silica gel to afford the product (0.20 g, 67%) as a clear oil. LCMS: [M+H]+ 229.3.
Step 3: N-ethyl-N-methylpyrrolidin-3-amine
TFA (1.7 mL, 22 mmol) was added to a stirring solution of tert-butyl 3-(ethyl(methyl)amino)pyrrolidine-1-carboxylate (0.20 g, 0.89 mmol) in DCM (2 mL) at RT. The reaction was allowed to stir at RT for 18 h. The volatiles were removed under a stream of air and the isolation of the desired product was achieved by a catch and release procedure using Biotage SCX2 silica gel to afford the product (0.12 g, quantitative yield). LCMS: [M+H]+=129.1.
Step 4: 1-(4-chlorophenyl)-N-ethyl-N-methylpyrrolidin-3-amine
A 30 mL vial was charged with N-ethyl-N-methylpyrrolidin-3-amine (0.12 g, 0.94 mmol), 1-bromo-4-chlorobenzene (0.22 g, 1.1 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.026 g, 0.028 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.049 g, 0.084 mmol) and cesium carbonate (0.92 g, 2.8 mmol). The vial was sealed with a cap and septum and then the reaction vessel was evacuated and backfilled with nitrogen. Toluene (6 mL) was added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction was heated in an aluminum block at 100° C. for >48 h. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0.5-10% DCM/MeOH+0.5% NH4OH) to afford the product (0.13 g, 56%). LCMS: [M+H]+=239.0.
Step 5: 6-(2-amino-5-(4-(3-(ethyl(methyl)amino)pyrrolidin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial was charged with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.20 mmol), 1-(4-chlorophenyl)-N-ethyl-N-methylpyrrolidin-3-amine (0.049 g, 0.21 mmol) and XPhos Pd G2 (0.015 g, 0.020 mmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. 1,4-dioxane (2 mL) and 1.3 M aqueous K3PO4 (0.38 mL, 0.49 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 95° C. for 18 h. The reaction mixture was cooled to RT, concentrated onto Celite® and purified by flash chromatography (0.5-9.5% MeOH/DCM+0.5% NH4OH). The product containing fractions were concentrated and further purified by reverse phase chromatography (Biotage SNAP C18; 5-50% MeCN/water+0.1% Formic Acid). Isolation of the title compound was achieved by a catch and release procedure using Biotage SCX2 silica gel to afford the product (24 mg, 27%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.56 (d, J=10.3 Hz, 1H), 7.4-7.5 (m, 2H), 7.36 (d, J=7.7 Hz, 2H), 6.59 (d, J=8.8 Hz, 2H), 6.11 (s, 2H), 3.47 (br d, J=1.8 Hz, 1H), 3.4-3.4 (m, 4H), 3.25 (dt, J=9.5, 2.4 Hz, 1H), 3.0-3.1 (m, 2H), 2.96 (t, J=6.5 Hz, 2H), 2.19 (s, 3H), 2.1-2.2 (m, 1H), 1.8-1.9 (m, 1H), 1.01 (t, J=7.2 Hz, 3H); LCMS: [M+H]+=460.5.
Step 1: tert-butyl 3-(2,2,2-trifluoro-N-methylacetamido)pyrrolidine-1-carboxylate
TFA (0.14 mL, 1.9 mmol) was activated with HATU (0.71 g, 1.9 mmol) and N,N-diisopropylethylamine (0.33 mL, 1.9 mmol) in DMF (1 mL) at RT. After stirring for 10 minutes the solution of activated acid was transferred by pipette to a vial containing a solution of 1-boc-3-methylaminopyrrolidine (0.25 g, 1.3 mmol) in DMF (1 mL) at RT. The reaction was allowed to stir at RT for 18 h and the reaction mixture was then concentrated onto Celite®. Purification by flash chromatography (25-75% EtOAC/hexanes) to afford the product (0.24 g, 64%) as a clear oil. LCMS: [M+H]+=297.3.
Step 2: tert-butyl 3-(methyl(2,2,2-trifluoroethyl)amino)pyrrolidine-1-carboxylate
A 2M solution of borane dimethyl sulfide complex in THF (1.2 mL, 2.4 mmol) was added dropwise to a solution of tert-butyl 3-(2,2,2-trifluoro-N-methylacetamido)pyrrolidine-1-carboxylate (0.24 g, 0.80 mmol) in THF (6 mL) at 0° C. The ice bath was removed and the reaction mixture was heated to 60° C. for 4 h. After cooling to RT the reaction was carefully quenched with the dropwise addition of saturated NaHCO3 (Aq.). Concentrating to dryness afforded the product (0.21 g, 94%) that was of sufficient purity to carry forward to the next step. LCMS: [M+H]+=282.2.
Step 3: N-methyl-N-(2,2,2-trifluoroethyl)pyrrolidin-3-amine
TFA (1.4 mL, 19 mmol) was added to a stirring solution of tert-butyl 3-(methyl(2,2,2-trifluoroethyl)amino)pyrrolidine-1-carboxylate (0.21 g, 0.75 mmol) in DCM (2 mL) at RT. The reaction was allowed to stir at RT for 18 h. The volatiles were removed under a stream of air and the isolation of the desired product was achieved by a catch and release procedure using Biotage SCX2 silica gel to afford the product (0.14 g, quantitative yield). LCMS: [M+H]+ 183.1.
Step 4: 1-(4-chlorophenyl)-N-methyl-N-(2,2,2-trifluoroethyl)pyrrolidin-3-amine
A procedure analogous to Example 108, Step 1 using N-methyl-N-(2,2,2-trifluoroethyl)pyrrolidin-3-amine (0.14 g, 0.77 mmol), 1-bromo-4-chlorobenzene (0.18 g, 0.92 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.021 g, 0.023 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.040 g, 0.069 mmol) and Cs2CO3 (0.75 g, 2.3 mmol) afforded the product (0.13 g, 56%). LCMS: [M+H]+=293.1.
Step 5: 6-(2-amino-6-fluoro-5-(4-(3-(methyl(2,2,2-trifluoroethyl)amino)pyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.20 mmol), 1-(4-chlorophenyl)-N-methyl-N-(2,2,2-trifluoroethyl)pyrrolidin-3-amine (0.060 g, 0.21 mmol) and XPhos Pd G2 (0.015 g, 0.020 mmol), 1,4-dioxane (2 mL), 1.3 M aqueous K3PO4 (0.38 mL, 0.49 mmol) afforded the title compound (9 mg, 9%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.56 (d, J=10.3 Hz, 1H), 7.4-7.5 (m, 2H), 7.37 (br d, J=8.1 Hz, 2H), 6.60 (d, J=8.7 Hz, 2H), 6.11 (s, 2H), 3.5-3.5 (m, 1H), 3.4-3.4 (m, 5H), 3.2-3.3 (m, 3H), 3.1-3.1 (m, 1H), 2.96 (br t, J=6.5 Hz, 2H), 2.44 (s, 3H), 2.2-2.2 (m, 1H), 1.8-1.9 (m, 1H); LCMS: [M+H]+=514.5.
Step 1: tert-butyl (R)-3-((2-methoxyethyl)(methyl)amino)pyrrolidine-1-carboxylate
A mixture of (S)-1-Boc-3-methanesulfonyloxy-pyrrolidine (0.28 g, 1.1 mmol), N-(2-methoxyethyl)methylamine (0.29 mL, 2.6 mmol) and N,N-diisopropylethylamine (0.50 mL, 2.6 mmol) was heated to 70° C. in a sealed tube for 18 h and then to 85° C. for >48 h. The reaction mixture was diluted with DCM and concentrated onto Celite®. Purification by flash chromatography [0.5-7.5% MeOH/DCM+0.5% NH4OH] to afford the product (0.23 g, 83%) as a clear oil. LCMS: [M+H]+=259.4.
Step 2: (R)—N-(2-methoxyethyl)-N-methylpyrrolidin-3-amine
TFA (1.7 mL, 22 mmol) was added to a stirring solution of tert-butyl (R)-3-((2-methoxyethyl)(methyl)amino)pyrrolidine-1-carboxylate (0.23 g, 0.88 mmol) in DCM (2 mL) at RT. The reaction was allowed to stir at RT for 18 h. The volatiles were removed under a stream of air and the isolation of the desired product was achieved by a catch and release procedure using Biotage SCX2 silica gel to afford the product (0.14 g, quantitative yield). LCMS: [M+H]+=159.3.
Step 3: (R)-1-(4-chlorophenyl)-N-(2-methoxyethyl)-N-methylpyrrolidin-3-amine
A procedure analogous to Example 108, Step 1 using (R)—N-(2-methoxyethyl)-N-methylpyrrolidin-3-amine (0.14 g, 0.89 mmol), 1-bromo-4-chlorobenzene (0.20 g, 1.1 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.024 g, 0.027 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.046 g, 0.080 mmol) and Cs2CO3 (0.87 g, 2.7 mmol) afforded the product (0.14 g, 61%). LCMS: [M+H]+=269.3.
Step 4: (R)-6-(2-amino-6-fluoro-5-(4-(3-((2-methoxyethyl)(methyl)amino)pyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.20 mmol), (R)-1-(4-chlorophenyl)-N-(2-methoxyethyl)-N-methylpyrrolidin-3-amine (0.055 g, 0.21 mmol), XPhos Pd G2 (0.015 g, 0.020 mmol), 1,4-dioxane (2 mL) and 1.3 M aqueous K3PO4 (0.38 mL, 0.49 mmol) afforded the title compound (0.037 g, 39%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.56 (d, J=10.3 Hz, 1H), 7.4-7.5 (m, 2H), 7.36 (d, J=7.9 Hz, 2H), 6.59 (d, J=8.7 Hz, 2H), 6.11 (s, 2H), 3.4-3.5 (m, 7H), 3.25 (s, 4H), 3.1-3.2 (m, 1H), 3.0-3.1 (m, 1H), 2.96 (br t, J=6.5 Hz, 2H), 2.60 (q, J=6.1 Hz, 2H), 2.25 (s, 3H), 2.17 (ddd, J=11.7, 6.8, 4.5 Hz, 1H), 1.8-1.9 (m, 1H); LCMS: [M+H]+=490.5.
Step 1: tert-butyl (R)-3-((cyclopropylmethyl)(methyl)amino)pyrrolidine-1-carboxylate
A mixture of tert-butyl (S)-3-((methylsulfonyl)oxy)pyrrolidine-1-carboxylate (0.290 g, 1.09 mmol), 1-cyclopropyl-N-methylmethanamine (0.233 g, 2.73 mmol) and N,N-diisopropylethylamine (0.476 mL, 2.73 mmol) was heated to 70° C. in a sealed tube for 18 h and then 85° C. for 48 h then at RT for 2 d. The vial was unsealed and diluted with CH2Cl2. The mixture was loaded onto Celite® and purified by flash chromatography (0.5-9.5% MeOH/DCM+0.5% NH4OH; ninhydrin stain) to afford the product (0.727 mmol, 67%) as a clear oil. LCMS: [M+H]+=255.37.
Step 2: (R)—N-(cyclopropylmethyl)-N-methylpyrrolidin-3-amine
TFA (1.39 mL, 18.2 mmol) was added to a stirring solution of tert-butyl (R)-3-((cyclopropylmethyl)(methyl)amino)pyrrolidine-1-carboxylate (0.185 g, 0.727 mmol) in CH2Cl2 (2 ml) at RT. The reaction was allowed to stir at RT for 18 h. A catch and release protocol with SCX-2 silica gel afforded the product (157 mg, 140%). LCMS: [M+H]+=155.26.
Step 3: (R)-1-(4-chlorophenyl)-N-(cyclopropylmethyl)-N-methylpyrrolidin-3-amine
A procedure analogous to Example 108, Step 1 using (R)—N-(cyclopropylmethyl)-N-methylpyrrolidin-3-amine (0.120 g, 0.778 mmol), 1-bromo-4-chlorobenzene (0.179 g, 0.934 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.021 g, 0.023 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.041 g, 0.070 mmol) and Cs2CO3 (0.760 g, 2.33 mmol) afforded the product (103 mg, 50%). LCMS: [M+H]+=265.01.
Step 4: (R)-6-(2-amino-5-(4-(3-((cyclopropylmethyl)(methyl)amino)pyrrolidin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.196 mmol), (R)-1-(4-chlorophenyl)-N-(cyclopropylmethyl)-N-methylpyrrolidin-3-amine (0.054 g, 0.205 mmol) and XPhos Pd G2 (0.015 g, 0.020 mmol) and aqueous K3PO4 (0.376 mL of a 1.3 M solution, 0.489 mmol) afforded the title compound (22 mg, 23%). 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.44 (d, J=10.1 Hz, 1H), 7.3-7.4 (m, 2H), 7.25 (d, J=7.9 Hz, 2H), 6.47 (d, 2H, J=8.8 Hz), 5.99 (s, 2H), 3.3-3.4 (m, 5H), 3.1-3.2 (m, 1H), 2.9-3.0 (m, 2H), 2.84 (br t, J=6.5 Hz, 2H), 2.19 (s, 3H), 2.0-2.1 (m, 1H), 1.7-1.8 (m, 1H), 0.7-0.8 (m, 1H), 0.3-0.4 (m, 2H), 0.0-0.0 (m, 2H); LCMS: [M+H]+=486.2.
Step 1: tert-butyl 3-(N-methylisobutyramido)pyrrolidine-1-carboxylate
Isobutyryl chloride (0.156 mL, 1.50 mmol) was dropwise to a vial containing a solution of 1-Boc-3-Methylaminopyrrolidine (0.250 g, 1.25 mmol) and Et3N (0.209 mL, 1.50 mmol) in CH2Cl2 (5 mL) at RT. The reaction was allowed to stir at RT overnight. The reaction mixture was concentrated onto Celite® and purified by flash chromatography (0.5-9.5% DCM/MeOH+0.5% NH4OH; ninhydrin) to afford the product (368 mg, quant. yield). LCMS: [M+H]+=215.03.
Step 2: tert-butyl 3-(isobutyl(methyl)amino)pyrrolidine-1-carboxylate
Borane dimethyl sulfide complex solution, 2 M in THF (2.04 mL, 4.08 mmol) was added carefully to a cooled (0° C.) solution of tert-butyl 3-(N-methylisobutyramido)pyrrolidine-1-carboxylate (0.368 g, 1.36 mmol) in THF (6 mL) while stirring under nitrogen. The ice bath was removed and the reaction mixture was heated to 60° C. for 4 h. After cooling to RT the reaction was quenched with the dropwise addition of saturated aqueous NaHCO3 (q) (evolution of gas and exothermic). The reaction was diluted with CH2Cl2 and water and the product was isolated by catch and release with SCX-2 silica gel to afford the product (274 mg, 79%) as a clear oil. LCMS: [M+H]+=257.41.
Step 3: N-isobutyl-N-methylpyrrolidin-3-amine
A procedure analogous to Example 61, Step 2 using TFA (2.05 mL, 26.8 mmol) and tert-butyl 3-(isobutyl(methyl)amino)pyrrolidine-1-carboxylate (0.275 g, 1.07 mmol) afforded the product (1.17 mmol, quant. yield). LCMS [M+H]+=157.22.
Step 4: 1-(4-chlorophenyl)-N-isobutyl-N-methylpyrrolidin-3-amine
A procedure analogous to Example 108, Step 1 using N-isobutyl-N-methylpyrrolidin-3-amine (0.170 g, 1.09 mmol), 1-bromo-4-chlorobenzene (0.250 g, 1.31 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.030 g, 0.033 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.057 g, 0.098 mmol) and Cs2CO3 (1.06 g, 3.26 mmol) afforded the title compound (0.322 mmol, 30%). LCMS:
Step 5: 6-(2-amino-6-fluoro-5-(4-(3-(isobutyl(methyl)amino)pyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.196 mmol), 1-(4-chlorophenyl)-N-isobutyl-N-methylpyrrolidin-3-amine (0.055 g, 0.205 mmol) and XPhos Pd G2 (0.015 g, 0.020 mmol) and aqueous K3PO4 (0.376 mL of a 1.3 M solution, 0.489 mmol) afforded the title compound (31 mg, 33%). 1H NMR (500 MHz, DMSO-d6) δ 7.8-7.9 (m, 2H), 7.48 (d, J=10.3 Hz, 1H), 7.4-7.4 (m, 2H), 7.29 (d, J=7.7 Hz, 2H), 6.52 (d, J=8.8 Hz, 2H), 6.03 (s, 2H), 3.3-3.4 (m, 5H), 3.1-3.2 (m, 1H), 3.0-3.0 (m, 2H), 2.88 (t, J=6.5 Hz, 2H), 2.0-2.1 (m, 6H), 1.6-1.8 (m, 2H), 0.80 (dd, J=6.6, 1.5 Hz, 6H); LCMS: [M+H]+=488.5.
Step 1: 1-(4-chlorophenyl)-4-fluoropiperidine
A procedure analogous to Example 108, Step 1 using 4-fluoropiperidine hydrochloride (0.182 g, 1.31 mmol), 1-bromo-4-chlorobenzene (0.250 g, 1.31 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.036 g, 0.039 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.068 g, 0.118 mmol) and Cs2CO3 (1.49 g, 4.57 mmol) afforded the product (253 mg, 91%). LCMS: [M+H]+=214.05.
Step 2: 6-(2-amino-6-fluoro-5-(4-(4-fluoropiperidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (0.075 g, 0.196 mmol), 1-(4-chlorophenyl)-4-fluoropiperidine (0.044 g, 0.205 mmol), XPhos Pd G2 (0.015 g, 0.020 mmol) and aqueous K3PO4 (0.376 mL of a 1.3 M solution, 0.489 mmol) afforded the title compound (9.0 mg, 11%) as a tan powder. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 2H), 7.59 (d, J=10.1 Hz, 1H), 7.4-7.5 (m, 2H), 7.41 (d, J=7.8 Hz, 2H), 7.01 (d, J=8.9 Hz, 2H), 6.18 (s, 2H), 4.8-4.9 (m, 1H), 3.4-3.4 (m, 5H), 3.1-3.2 (m, 2H), 2.96 (t, J=6.5 Hz, 2H), 1.9-2.0 (m, 2H), 1.7-1.9 (m, 2H); LCMS: [M+H]+=435.5.
Step 1: 3-(4-chlorophenyl)-1-isopropyl-3-methylpyrrolidine
A solution of 3-(4-chlorophenyl)-3-methylpyrrolidine·HCl (120 mg, 0.517 mmol), Et3N (0.14 mL, 1.0 mmol) and acetone (0.077 mL, 1.0 mmol) in CH2Cl2 (14 mL) was stirred for 2 h at rt before NaBH(OAc)3 (274 mg, 1.29 mmol) was added in one portion at RT. Stirring was continued at that temperature for 1 d and saturated aq NaHCO3, was added. The organic layer was separated and concentrated to afford crude product that was used without further purification.
Step 2. 6-(2-amino-6-fluoro-5-(4-(1-isopropyl-3-methylpyrrolidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A, heating a sealed and degassed mixture of K3PO4 (161 mg, 0.757 mmol), XPhos-Pd-G2 (20 mg, 0.025 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.303 mmol), 3-(4-chlorophenyl)-1-isopropyl-3-methylpyrrolidine (50% of the material from Step 1, 60 mg, 0.25 mmol), H2O (6 mL) and MeCN (9 mL) a microwave reactor at 100° C. for 1.5 h, afforded the title compound as a pale yellow solid (30 mg, 26% yield for 2 steps). 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.64 (d, J=10.2 Hz, 1H), 7.43-7.58 (m, 4H), 7.32-7.43 (m, 2H), 6.30 (s, 2H), 3.39-3.49 (m, 2H), 2.96 (br t, J=6.4 Hz, 1H), 2.30-3.25 (br m, 4H), 2.07-2.22 (m, 1H), 1.85-1.99 (m, 1H), 1.30-1.61 (m, 1H), 1.18-1.40 (m, 4H), 0.97-1.17 (br s, 6H); LCMS: [M+H]+=459.53.
Step 1: 6-(5-(4-((1S,5R)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 43, Step 5 using (1S,5R)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (60 mg, 0.252 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (126 mg, 0.328 mmol) afforded the product as a beige solid (110 mg, 95% yield based on purity of 90%); LCMS: [M+H]+=415.52.
Step 2: Preparation on 6-(2-amino-6-fluoro-5-(4-((1S,5R)-3-(2-methoxyethyl)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one)
A procedure analogous to that of Example 90, Step 2 using 6-(5-(4-((1S,5R)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.109 mmol) and 2-bromoethyl methyl ether (0.031 ml, 0.326 mmol) overnight at RT. The reaction mixture concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-4% MeOH and 0-0.4% NH4OH), followed by reverse phase chromatography (C18; 0-30% acetonitrile/water) afforded the title compound as an off white solid (19 mg, 35%). 1H NMR (500 MHz, DMSO-d6) δ 8.02-8.02 (m, 1H), 7.92-7.96 (m, 1 H), 7.88-7.92 (m, 1H), 7.58-7.64 (m, 1H), 7.41-7.48 (m, 4H), 7.15-7.21 (m, 2H), 6.27 (s, 2H), 3.44-3.47 (m, 2H), 3.29-3.32 (m, 1H), 3.23-3.26 (m, 3H), 3.15-3.18 (m, 2H), 3.02-3.07 (m, 1H), 2.92-2.98 (m, 2H), 2.61-2.66 (m, 2H), 2.55-2.59 (m, 1H), 2.41-2.48 (m, 1H), 1.75-1.82 (m, 1H), 1.29-1.33 (m, 1H), 0.72-0.78 (m, 1H); LCMS: [M+H]+=473.62.
Step 1: tert-butyl 4-(4-(6-amino-2-chloro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate
A procedure analogous to that of Example 43, Step 5 using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.142 mmol) and 4-(N-Boc-piperidino)phenylboronic acid pinacol ester (71.4 mg, 0.184 mmol) afforded the product as an off white solid (66 mg, 87%). LCMS: [M+H]+=533.60.
Step 2: 6-(2-amino-6-chloro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A mixture of tert-butyl 4-(4-(6-amino-2-chloro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate (66 mg, 0.124 mmol) in MeOH (3.5 mL) with HCl solution 4.0 M in dioxane (0.619 mL, 2.48 mmol) at RT for 2.5 h. The reaction mixture was concentrated and filtered through a Waters PoraPak CX column to afford the title compound as a white powder (52 mg, 97%); LCMS: [M+H]+=433.5.
Step 3: 6-(2-amino-6-chloro-5-(4-(1-(2-methoxyethyl)piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 90, Step 2 stirring 6-(2-amino-6-chloro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (52 mg, 0.120 mmol), and 2-bromoethyl methyl ether (0.034 ml, 0.360 mmol) overnight at RT afforded the title compound as an off white powder (36.5 mg, 59%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 3H), 7.3-7.3 (m, 2H), 6.3-6.4 (m, 2H), 3.5-3.5 (m, 2H), 3.4-3.4 (m, 3H), 3.26 (s, 3H), 3.0-3.1 (m, 2H), 2.9-3.0 (m, 2H), 2.5-2.6 (m, 2H), 2.0-2.3 (m, 2H), 1.7-1.8 (m, 2H), 1.6-1.7 (m, 2H); LCMS: [M+H]+=491.54.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-4-hydroxypiperidine-1-carboxylate
A procedure analogous to Example 43, Step 5 using tert-butyl 4-(4-chlorophenyl)-4-hydroxypiperidine-1-carboxylate (50 mg, 0.160 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (86 mg, 0.225 mmol) K3PO4 (102 mg, 0.481 mmol), XPhos Pd G2 (16.40 mg, 0.021 mmol), 1-butanol (4 mL) and water (0.600 mL) afforded the product as a pale yellow solid (75 mg, 88%). LCMS: [M+H]+=533.60.
Step 2: 6-(2-Amino-6-fluoro-5-(4-(4-hydroxypiperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A solution of 4.0 M HCl in dioxane (0.704 ml, 2.82 mmol) was added to a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-4-hydroxypiperidine-1-carboxylate (0.035 mL, 0.141 mmol) in MeOH (4 mL). The reaction mixture was stirred at RT for 2.5 h then concentrated to dryness. The residue was passed through a Waters PoraPak CX column to afford the title compound as a white powder (74 mg, 83% yield based on purity of 68%); LCMS: [M+H]+=415.45.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-hydroxy-1-(2-methoxyethyl)piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 90, Step 2 stirring 6-(2-amino-6-fluoro-5-(4-(4-hydroxypiperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (74 mg, 0.116 mmol) and 2-bromoethyl methyl ether (0.033 mL, 0.349 mmol) at RT overnight afforded the title compound as an off white powder (30 mg, 50%). 1H NMR (500 MHz, DMSO-d6) δ 7.93-7.98 (m, 1H), 7.89-7.93 (m, 1H), 7.62-7.68 (m, 1H), 7.45-7.55 (m, 6H), 6.24-6.36 (m, 2H), 4.77-4.88 (m, 1H), 3.45-3.49 (m, 2H), 3.40-3.44 (m, 2H), 3.36-3.37 (m, 2H), 3.29-3.32 (m, 2H), 3.22-3.26 (m, 3H), 2.93-3.00 (m, 2H), 2.64-2.73 (m, 2H), 2.41-2.49 (m, 2H), 1.88-1.99 (m, 2H), 1.54-1.64 (m, 2H); LCMS: [M+H]+=491.54.
Step 1: tert-butyl 4-(4-(6-amino-2-chloro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate
A procedure analogous to that of Example 43, step 5, using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.142 mmol) and tert-butyl 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]tetrahydro-1(2H)-pyrazinecarboxylate (71.6 mg, 0.184 mmol) afforded the product as a pale yellow solid (58 mg, 77%). LCMS: [M+H]+=534.60.
Step 2: 6-(2-amino-6-chloro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 167, Step 2 stirring tert-butyl 4-(4-(6-amino-2-chloro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate (58 mg, 0.109 mmol) in MeOH (3.5 mL) with HCl solution (4.0 M in dioxane, 0.543 mL, 2.17 mmol) overnight at RT. The reaction mixture was concentrated and filtered through a Waters PoraPak CX column to afford the title compound as a white powder (53 mg, 96% yield base on purity of 85%); LCMS: [M+H]+=434.44.
Step 3: 6-(2-amino-6-chloro-5-(4-(4-(2,2-difluoroethyl)piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 90, Step 2 heating 6-(2-amino-6-chloro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (53 mg, 0.104 mmol, purity 85%) and 2,2-difluoroethyl trifluoromethanesulfonate (0.041 ml, 0.311 mmol) at 80° C. for 3 h afforded the title compound as an off white powder (33 mg, 61%). 1H NMR (500 MHz, DMSO-d6) δ 7.94-7.99 (m, 1H), 7.89-7.94 (m, 1H), 7.45-7.51 (m, 2H), 7.37-7.40 (m, 1H), 7.30-7.36 (m, 2H), 6.97-7.03 (m, 2H), 6.09-6.34 (m, 3H), 3.42-3.45 (m, 2H), 3.18-3.23 (m, 4H), 2.94-3.01 (m, 2H), 2.77-2.87 (m, 2H), 2.67-2.74 (m, 4H); LCMS: [M+H]+=498.56.
A procedure analogous to that of Example 43, step 5 using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.113 mmol), 4-(4-Isopropylpiperazinyl)phenylboronic acid, pinacol ester (48.7 mg, 0.147 mmol) afforded the title compound as a white powder (31 mg, 55%). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.92-7.98 (m, 1H), 7.86-7.92 (m, 1H), 7.45-7.49 (m, 1H), 7.43-7.45 (m, 1H), 7.35-7.38 (m, 1H), 7.28-7.34 (m, 2H), 6.93-7.01 (m, 2H), 6.19-6.28 (m, 2H), 3.39-3.43 (m, 2H), 3.13-3.21 (m, 4H), 2.92-2.99 (m, 2H), 2.66-2.74 (m, 1H), 2.56-2.65 (m, 4H), 0.98-1.06 (m, 6H); LCMS: [M+H]+=476.63.
Step 1: 1-(6-chloropyridin-3-yl)-4-isopropylpiperazine
A 20 mL vial was charged with 5-bromo-2-chloropyridine (500 mg, 2.60 mmol), 1-isopropylpiperazine (366 mg, 2.86 mmol), Pd2dba3 (71 mg, 0.078 mmol), Xantphos (135 mg, 0.234 mmol) and Cs2CO3 (2540 mg, 7.79 mmol). The vial was sealed with a cap and septum and then the reaction vessel was evacuated and backfilled with nitrogen. PhMe (15 mL) was added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction was heated in oil bath at 80° C. for 4 h and then at 100° C. for 19 h. The reaction mixture was concentrated and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) to afford the product as a red-orange gum (514 mg, 77% yield based on purity of 94%). LCMS: [M+H]+=240.10.
Step 2: 6-(6′-Amino-2′-fluoro-5-(4-isopropylpiperazin-1-yl)-[2,3′-bipyridin]-5′-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General method A by heating a sealed, degassed mixture of 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (51 mg, 0.13 mmol, 19.3 mg/mL) in 1,4-dioxane (2.6 mL), K3PO4 (81 mg, 0.380 mmol), 1-(6-chloropyridin-3-yl)-4-isopropylpiperazine (32.3 mg, 0.127 mmol, 94% purity), CH3CN (9 mL), H2O (6 mL) and XPhos-Pd-G2 (10 mg, 0.013 mmol) in a microwave reactor at 100° C. for 1.5 h afforded after workup and purification the title compound as an off white solid (18 mg, 29% yield based on purity of 97%). 1H NMR (500 MHz, DMSO-d6) δ 8.25 (d, J=2.8 Hz, 1H), 8.04 (br s, 1H), 7.95-8.02 (m, 1H), 7.55 (d, J=10.2 Hz, 1H), 7.50-7.53 (m, 1H), 7.33 (d, J=7.1 Hz, 1H), 7.30 (dd, J=8.9, 2.8 Hz, 1H), 6.31 (s, 2H), 3.32-3.38 (m, 2H), 3.06-3.16 (m, 4H), 2.83-2.90 (m, 2H), 2.56-2.67 (m, 1H), 2.48-2.55 (m, 4H), 0.94 (br d, J=6.4 Hz, 6H). LCMS: [M+H]+=479.54.
Step 1: tert-butyl 3-(4-bromophenyl)-2,5-dihydro-1H-pyrrole-1-carboxylate
A procedure similar to Example 1, General Method A heating a degassed, sealed mixture of 1-bromo-4-iodobenzene (500 mg, 1.77 mmol), 1-Boc-2,5-dihydro-1H-pyrrole-3-boronic acid, pinacol ester (521 mg, 1.77 mmol), Pd(PPh3)2Cl2 (124 mg, 0.177 mmol), MeCN (12 mL), Na2CO3 (438 mg, 3.53 mmol) and H2O (7 mL) in a microwave reactor at 80° C. for 45 min afforded the product as a white solid (426 mg, 74%). LCMS: [M−t−Bu+2H]+=268.17/270.20.
Step 2: 1-(4-bromophenyl)-6,6-difluoro-3-azabicyclo[3.1.0]hexane
An oven-dried microwave vial was charged with n-Bu4NBr (4.18 mg, 0.013 mmol), tert-butyl 3-(4-bromophenyl)-2,5-dihydro-1H-pyrrole-1-carboxylate (70 mg, 0.22 mmol) and anh. PhMe (2 mL). The sealed vial was filled with Ar and BrCF2SiMe3 (66 mg, 0.32 mmol) was added at RT. After briefly stirring at RT, the reaction was heated in an oil bath at 80° C. for 1.5 h and then at 100° C. for 19 h. The reaction was cooled to RT, quenched by an addition of MeOH and concentrated under reduced pressure to afford the product. LCMS: [M+H]+=274.19/276.15.
Step 3: 6-(2-amino-5-(4-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 1-(4-bromophenyl)-6,6-difluoro-3-azabicyclo[3.1.0]hexane (59 mg, 0.22 mmol), DME (10 mL), H2O (5 mL), PdCl2dppf (16 mg, 0.022 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.302 mmol) and Cs2CO3 (211 mg, 0.648 mmol) afforded the product as a tan solid (60 mg, 55% yield for 2 steps, based on purity of 89%). LCMS: [M+H]+=451.48.
Step 4: 6-(2-amino-5-(4-(6,6-difluoro-3-isopropyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
An anhydrous CH2Cl2 (20 mL) solution of 6-(2-amino-5-(4-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (60 mg, 0.12 mmol, 89% purity), acetone (0.018 mL, 0.24 mmol) and glacial acetic acid (0.014 mL, 0.24 mmol) was stirred at RT for 15 min before NaBH(OAc)3 (62.8 mg, 0.296 mmol) was added in one portion. Stirring was continued at RT for 2 d. MeOH was added, the reaction mixture was concentrated under reduced pressure and purified by flash chromatography (25 g SiO2, using CH2Cl2/MeOH/aq NH4OH 89/10/1 in CH2Cl2) followed by a filtration through a Waters PoraPak CX column to afford the title compound as an off-white solid (26 mg, 43% yield based on purity of 96%). 1H NMR (500 MHz, DMSO-d6) δ 7.86-7.89 (m, 1H), 7.84 (d, J=8.0 Hz, 1H), 7.59 (d, J=10.2 Hz, 1H), 7.47 (br d, J=7.7 Hz, 2H), 7.36-7.43 (m, 2H), 7.29 (br d, J=8.2 Hz, 2H), 6.27 (s, 2H), 3.48-3.55 (m, 1H), 3.31-3.37 (m, 2H), 3.11-3.18 (m, 1H), 2.88 (br t, J=6.4 Hz, 3H), 2.65-2.85 (m, 2H), 2.35-2.41 (m, 1H), 0.94 (br d, J=6.1 Hz, 6H). LCMS: [M+H]+=493.46.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate
A procedure similar to General Method A heating a mixture of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (320 mg, 0.904 mmol), tert-butyl 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]tetrahydro-1(2H)-pyrazinecarboxylate (421 mg, 1.08 mmol), Cs2CO3 (736 mg, 2.26 mmol) in a 3:1 mixture of DME/H2O (16 mL) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (66.1 mg, 0.090 mmol) in a microwave apparatus at 90° C. for 2.5 h afforded after workup and purification the product (349 mg, 72%) as a brown solid. LCMS: [M+H]+=536.61.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate (349 mg, 0.652 mmol) in CH2Cl2 (5.0 mL) was added TFA (1.50 ml, 19.6 mmol). The reaction was stirred at 40° C. for 16 h, then concentrated onto Celite® and purified by reverse phase eluting with 0-100% ACN/H2O. The desired fractions were collected, concentrated and dried under high vacuum to afford 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one-TFA (0.520 mmol, 80%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.61 (br s, 2H), 8.12 (br s, 1H), 7.61 (dd, J=10.1, 6.8 Hz, 2H), 7.41-7.47 (m, 3H), 7.04 (br d, J=8.8 Hz, 2H), 6.23 (s, 2H), 3.41 (br dd, J=6.7, 2.5 Hz, 2H), 3.37 (br d, J=5.3 Hz, 4H), 3.25 (br d, J=4.8 Hz, 4H), 2.93 (br t, J=6.4 Hz, 2H); LCMS: [M+H]+=436.51.
Step 1: tert-butyl 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidine]-1′-carboxylate
To a solution of bis(pinacolato)diboron (597 mg, 2.350 mmol), tert-butyl 6-bromo-4h-spiro[benzo[d][1,3]dioxine-2,4′-piperidine]-1′-carboxylate (602 mg, 1.567 mmol) in 1,4-dioxane (14 ml) was added KOAc (431 mg, 4.39 mmol) and the reaction mixture was degassed with argon for 15 minutes. Pd(dppf)Cl2 (172 mg, 0.235 mmol) was added and the reaction mixture was stirred at 90° C. for overnight. The reaction mixture was partitioned into DCM and water and the product was extracted using DCM. The organic phase was dried over MgSO4 and after filtration and concentration under vacuum, the crude product was dry loaded onto Celite® and purified by flash chromatography (0-30% hexanes/EtOAc) to afford the product (660.0 mg, 98%) as a colorless oil which solidify over time as a white powder which was used for the next step. LCMS: [M-tBu+H]+=376.39.
Step 2: tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-4H-spiro[benzo[d][1,3]dioxine-2, 4′-piperidine]-1′-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidine]-1′-carboxylate (323 mg, 0.750 mmol), tert-butyl 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidine]-1′-carboxylate (323 mg, 0.750 mmol) and 1,4-dioxane (5 mL) Cs2CO3 (611 mg, 1.87 mmol) in water (1 mL) and Pd(dppf)Cl2 (68.6 mg, 0.094 mmol) afforded the title compound (301.9 mg, 0.512 mmol, 82% yield) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.48-7.45 (m, 1H), 7.44 (s, 1H), 7.39-7.34 (m, 1H), 7.28 (s, 1H), 6.89 (d, J=8.6 Hz, 1H), 6.25 (s, 2H), 4.88 (s, 2H), 3.45-3.35 (br m, 6H), 2.97-2.91 (m, 2H), 1.85-1.77 (br m, 4H), 1.41 (s, 9H); [M+H]+=561.55.
Step 1: tert-butyl 4-oxo-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[chromane-2,4′-piperidine]-1′-carboxylate
To a solution of tert-butyl 6-bromo-4-oxospiro[chroman-2,4′-piperidine]-1′-carboxylate (607.9 mg, 1.53 mmol), bis(pinacolato)diboron (584 mg, 2.30 mmol) in 1,4-dioxane (14 ml) was added KOAc (422 mg, 4.30 mmol) and the reaction mixture was degassed with argon and Pd(dppf)Cl2 (168 mg, 0.230 mmol) was added. The reaction mixture was stirred at 90° C. for 16 h, then partitioned into CH2Cl2 and water and the product was extracted using CH2Cl2. The organic phase was dried over MgSO4 and after filtration and concentration under vacuum, the crude product was dry loaded onto Celite® and purified by flash chromatography (0-30% hexanes/EtOAc) to afford the product (596.7 mg, 88%) as a colorless oil which solidify over time as a white powder which was used for the next step. LCMS: [M-Me+H]+=429.36.
Step 2: tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-4-oxospiro[chromane-2,4′-piperidine]-1′-carboxylate
A procedure analogous to Example 43, Step 5 using tert-butyl 4-oxo-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[chromane-2,4′-piperidine]-1′-carboxylate (318 mg, 0.718 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (201 mg, 0.598 mmol) and 1,4-dioxane (3 mL). Then a solution of Cs2CO3 (584 mg, 1.79 mmol) in water (1 mL) and Pd(dppf)Cl2 (65.6 mg, 0.090 mmol) afforded the title compound (359.0 mg, 100%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.86 (s, 1H), 7.81-7.77 (m, 1H), 7.65 (d, J=10.3 Hz, 1H), 7.50-7.44 (m, 2H), 7.14 (d, J=8.6 Hz, 1H), 6.33 (s, 2H), 3.77-3.69 (m, 2H), 3.43-3.37 (m, 2H), 3.25-3.03 (m, 2H), 2.95 (s, 2H), 2.87 (s, 2H), 1.94-1.87 (m, 2H), 1.69-1.60 (m, 2H), 1.40 (s, 9H); [M+H]+=573.51.
Step 1: tert-butyl 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[chromane-2,4′-piperidine]-1′-carboxylate
To a solution of bis(pinacolato)diboron (600 mg, 2.364 mmol), tert-butyl 6-bromo-3,4-dihydrospiro[1-benzopyran-2,4′-piperidine]-1′-carboxylate (602.4 mg, 1.576 mmol) in 1,4-dioxane (14 ml) was added KOAc (433 mg, 4.41 mmol) and the reaction mixture was degassed with argon and Pd(dppf)Cl2 (173 mg, 0.236 mmol) was added. The reaction mixture was stirred at 90° C. for 16 h, then partitioned into CH2Cl2 and water and the product was extracted using CH2Cl2. The organic phase was dried over MgSO4 and after filtration and concentration under vacuum, the crude product was dry loaded onto Celite® and purified by flash chromatography (0-20% hexanes/EtOAc) to afford the product (603.9 mg, 89%) as a colorless oil which solidified into a white powder. LCMS: [M+H]+=430.41.
Step 2: tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)spiro[chromane-2,4′-piperidine]-1′-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[chromane-2,4′-piperidine]-1′-carboxylate (320 mg, 0.746 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1 (2H)-one (209 mg, 0.622 mmol), 1,4-dioxane (3 mL) Cs2CO3 (608 mg, 1.7 mmol) in water (1 mL) and Pd(dppf)Cl2 (68.2 mg, 0.093 mmol) afforded the title compound (359.0 mg, 98%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 2H), 7.28-7.23 (m, 2H), 6.82 (d, J=8.2 Hz, 1H), 6.20 (s, 2H), 3.76-3.65 (m, 2H), 3.40 (br d, J=2.6 Hz, 2H), 3.25-3.03 (m, 2H), 2.95 (s, 2H), 2.76 (s, 2H), 1.84-1.77 (m, 2H), 1.68 (br s, 2H), 1.57-1.49 (m, 2H), 1.41 (s, 10H); [M+H]+=559.60.
Step 1: tert-butyl 4-(2-cyano-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)piperidine-1-carboxylate
To a solution of tert-butyl 4-(4-bromo-2-cyanophenoxy)piperidine-1-carboxylate (601.2 mg, 1.58 mmol), bis(pinacolato)diboron (601 mg, 2.37 mmol), 1,4-dioxane (14 ml), KOAc (433 mg, 4.42 mmol) and Pd(dppf)Cl2 (174 mg, 0.237 mmol) afforded the product (757.8 mg, quantitative yield) as a slightly yellow viscous oil. LCMS: [M-tBu+H]+=373.30.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-cyanophenoxy)piperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 4-(2-cyano-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)piperidine-1-carboxylate (309 mg, 0.721 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1 (2H)-one (202 mg, 0.601 mmol), 1,4-dioxane (3 ml) and Cs2CO3 (587 mg, 1.80 mmol) in water (1 mL) and Pd(dppf)Cl2 (66.0 mg, 0.090 mmol) afforded the title compound (340.9 mg, 97%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (d, J=2.3 Hz, 2H), 7.91 (d, J=8.1 Hz, 1H), 7.83 (br d, J=9.3 Hz, 1H), 7.71 (d, J=10.3 Hz, 1H), 7.48 (dd, J=7.9, 1.6 Hz, 1H), 7.45 (s, 1H), 7.39 (d, J=9.2 Hz, 1H), 6.42-6.35 (m, 2H), 4.88-4.82 (m, 1H), 3.62-3.54 (m, 2H), 3.44-3.38 (m, 2H), 3.32-3.27 (m, 2H), 2.98-2.92 (m, 2H), 1.96-1.88 (m, 2H), 1.67-1.59 (m, 2H), 1.41 (s, 9H); LCMS: [M+H]+=558.39.
Step 1: tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate
In a vial were added tert-butyl 4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate (516 mg, 1.13 mmol), 5-bromo-3-chloro-6-fluoropyridin-2-amine (255 mg, 1.13 mmol) and 1,4-dioxane (8 mL). Then a solution of Cs2CO3 (1105 mg, 3.39 mmol) in water (1 mL) was added at RT. The reaction mixture was degassed under argon and Pd(dppf)Cl2 (124 mg, 0.170 mmol) was added and the reaction mixture was heated at 90° C. for 16 h. The mixture was dry loaded onto Celite® and purified by flash chromatography (0-10% DCM/MeOH) to afford the product (313.4 mg, 58%) as a grey powder. LCMS: [M+H]+=475.33.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-y)pyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate
A procedure analogous to that of Example 43, Step 5 using tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate (156.8 mg, 0.330 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (108 mg, 0.396 mmol) and 1,4-dioxane (4 mL), K3PO4 (210 mg, 0.991 mmol) in water (1 mL), and XPhos Pd G2 (26.0 mg, 0.033 mmol) afforded the title compound (133.4 mg, 66%) as a dark beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.93 (m, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.80 (s, 2H), 7.72 (d, J=10.1 Hz, 1H), 7.61 (d, J=8.3 Hz, 1H), 7.48 (d, J=7.9 Hz, 1H), 7.45 (s, 1H), 6.41 (s, 2H), 3.49-3.38 (m, 6H), 2.97-2.92 (m, 2H), 2.87-2.81 (m, 4H), 1.43 (s, 9H); LCMS: [M+H]+=586.53.
Step 1: tert-butyl (2R,4S,6S)-4-(4-bromophenoxy)-2,6-dimethylpiperidine-1-carboxylate
A procedure analogous to Example 147, Step 1 using tert-butyl (2R,4r,6S)-rel-4-hydroxy-2,6-dimethylpiperidine-1-carboxylate (510 mg, 2.224 mmol), 4-bromophenol (385 mg, 2.22 mmol), PPh3 (642 mg, 2.45 mmol) in anhydrous THF (10 mL) and diisopropyl azodicarboxylate (0.525 mL, 2.67 mmol) afforded the product (642.0 mg, 75%) and 4-bromophenol with a ratio of 4:6 and as a slightly yellow oil which was used for the next step. LCMS: [M-tBu+H]+=328.38.
Step 2: tert-butyl (2R,4S,6S)-4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)-2,6-dimethylpiperidine-1-carboxylate
A procedure analogous to Example 43, Step 5 using tert-butyl (2R,4s,6S)-4-(4-bromophenoxy)-2,6-dimethylpiperidine-1-carboxylate (188 mg, 0.488 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (144 mg, 0.376 mmol) and 1,4-dioxane (3 mL), K3PO4 (239 mg, 1.13 mmol) in water (1 mL) and XPhos Pd G2 (29.6 mg, 0.038 mmol) afforded the title compound (71.8 mg, 34% yield) as a dark oil which was used for the next step. LCMS: [M+H]+=561.55.
Step 3: 6-(2-amino-5-(4-(((2R,4S,6S)-2,6-dimethylpiperidin-4-yl)oxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 144, Step 3 using tert-butyl (2R,4s,6S)-4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)-2,6-dimethylpiperidine-1-carboxylate (71.8 mg, 0.128 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (29.0 mg, 44%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.52-7.42 (m, 4H), 7.04 (br d, J=8.7 Hz, 2H), 6.25 (s, 2H), 4.89-4.83 (m, 1H), 3.46-3.37 (m, 5H), 2.98-2.92 (m, 2H), 2.10-2.03 (m, 2H), 1.76-1.65 (m, 2H), 1.24 (br d, J=6.4 Hz, 8H); LCMS: [M+H]+=461.49.
Step 1: tert-butyl 4-(4-bromo-2-fluorophenoxy)piperidine-1-carboxylate
A procedure analogous to Example 147, Step 1 using 4-bromo-2-fluorophenol (517 mg, 2.71 mmol), tert-butyl 4-hydroxy-1-piperidinecarboxylate (495 mg, 2.459 mmol), PPh3 (710 mg, 2.71 mmol), THF (10 mL) and diethyl azodicarboxylate 40 wt % in toluene (2.24 mL, 4.92 mmol) afforded the title compound (764.0 mg, 83%) and 4-bromo-2-fluorophenol with a ratio of 84:16 and as a slightly yellow oil which was used without further purification for the next step. LCMS: [M−Me+H]+=361.27.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-fluorophenoxy)piperidine-1-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 4-(4-bromo-2-fluorophenoxy)piperidine-1-carboxylate (175 mg, 0.468 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (138 mg, 0.360 mmol), 1,4-dioxane (3 mL), K3PO4 (229 mg, 1.08 mmol) in water (1 mL) and XPhos Pd G2 (28.3 mg, 0.036 mmol) afforded the product (49.0 mg, 25%) as a beige powder. LCMS: [M−tBu+H]+=495.42.
Step 3: 6-(2-amino-6-fluoro-5-(3-fluoro-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-fluorophenoxy)piperidine-1-carboxylate (49.0 mg, 0.089 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the product (18.2 mg, 43%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.66 (d, J=10.1 Hz, 1H), 7.48 (d, J=8.1 Hz, 1H), 7.47-7.40 (m, 2H), 7.34-7.28 (m, 1H), 7.27-7.21 (m, 1H), 6.32 (s, 2H), 4.50-4.40 (m, 1H), 3.44-3.39 (m, 2H), 2.96 (br s, 4H), 2.62-2.53 (m, 2H), 1.97-1.89 (m, 2H), 1.55-1.45 (m, 2H); LCMS: [M+H]+=451.41.
Step 1: tert-butyl (R)-3-(4-bromophenoxy)piperidine-1-carboxylate
To a suspension of NaH, 60% in mineral oil (2.59 g, 64.8 mmol) in DMF (20 mL) was added tert-butyl-(3R)-3-hydroxypiperidine-1-carboxylate (2.5 g, 12.42 mmol) and 1-bromo-4-fluorobenzene (5.46 ml, 49.7 mmol) at RT. The mixture was stirred at 90° C. for 4 hours and then evaporated to dryness. The residue is taken up in 50 mL of ice-water and extracted with DCM. The organic extracts were dried over MgSO4 and after filtration, the solvents were evaporated to dryness, to give the crude material which was dry loaded onto Celite® and purified by flash chromatography (0-30% DCM/MeOH) to afford the product (2.2 g, 50%) as a yellow oil which was used for the next step. LCMS: [M−tBu+H]+=302.15.
Step 2: tert-butyl (R)-3-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)piperidine-1-carboxylate
To a solution of tert-butyl (R)-3-(4-bromophenoxy)piperidine-1-carboxylate (2.2 g, 6.18 mmol), bis(pinacolato)diboron (2.35 g, 9.26 mmol) in 1,4-dioxane (14 ml) was added KOAc (1.70 g, 17.3 mmol) and the reaction mixture was degassed with argon for 15 min. Then Pd(dppf)Cl2 (0.678 g, 0.926 mmol) was added and the reaction mixture was stirred at 90° C. for 16 h. The reaction mixture was partitioned into DCM and water and the product was extracted using DCM. The organic phase was dried over MgSO4 and after filtration and concentration under vacuum, the crude product was dry loaded onto Celite® and purified by flash chromatography (0-20% hexanes/EtOAc) to afford the product (2.00 g, 80%) as a colorless oil which solidified to a white powder. LCMS: [M−tBu+H]+=348.55.
Step 3: tert-butyl (R)-3-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenoxy)piperidine-1-carboxylate
In a vial were added tert-butyl (R)-3-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)piperidine-1-carboxylate (369 mg, 0.916 mmol), 5-bromo-3-chloro-6-fluoropyridin-2-amine (206.4 mg, 0.916 mmol) and 1,4-dioxane (4 mL). Then a solution of Cs2CO3 (895 mg, 2.75 mmol) in Water (1 ml) was added at RT and the reaction mixture was degassed under argon for 15 minutes. Then Pd(dppf)Cl2 (100 mg, 0.137 mmol) was added and the reaction mixture was heated at 90° C. for overnight. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography [0-10% DCM/MeOH] to afford the product (94.1 mg, 24%) as a dark beige powder which was used for the next step. [M−tBu+H]+ 366.15.
Step 4: tert-butyl (R)-3-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate
A procedure analogous to Example 143, Step 5 using tert-butyl (R)-3-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)phenoxy)piperidine-1-carboxylate (94.1 mg, 0.223 mmol), 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (78 mg, 0.268 mmol), 1,4-dioxane (3 mL), K3PO4 (142 mg, 0.669 mmol) in water (1 mL) and XPhos Pd G2 (17.55 mg, 0.022 mmol) afforded the product (35.0 mg, 27%) as a dark beige powder which was used for the next step. LCMS: [M+H]+=551.47.
Step 5: rac-(R)-6-(2-amino-6-fluoro-5-(4-(piperidin-3-yloxy)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 144, Step 3 using tert-butyl (R)-3-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate (35.0 mg, 0.064 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (18.2 mg, 59%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (br s, 1H), 7.60 (dd, J=10.1, 6.3 Hz, 2H), 7.46-7.40 (m, 3H), 6.98 (d, J=8.8 Hz, 2H), 6.23 (s, 2H), 4.33-4.25 (m, 1H), 3.43-3.38 (m, 2H), 3.16-3.08 (m, 1H), 2.95-2.89 (m, 2H), 2.82-2.75 (m, 1H), 2.58-2.52 (m, 2H), 2.07-1.98 (m, 1H), 1.73-1.64 (m, 1H), 1.56-1.40 (m, 2H); LCMS: [M+H]+=451.48.
A procedure analogous to Example 144, Step 3 using tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidine]-1′-carboxylate (285 mg, 0.508 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL), TFA (2 mL, 26.1 mmol) afforded the title compound (166.1 mg, 67%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.3 Hz, 1H), 7.49-7.42 (m, 2H), 7.35 (br d, J=8.3 Hz, 1H), 7.26 (s, 1H), 6.86 (d, J=8.6 Hz, 1H), 6.24 (s, 2H), 4.84 (s, 2H), 3.44-3.38 (m, 2H), 2.95 (s, 2H), 2.75 (br s, 4H), 1.74 (br s, 4H); LCMS: [M+H]+=461.49.
A procedure similar to Example 144, Step 3 using tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-4-oxospiro[chromane-2,4′-piperidine]-1′-carboxylate (339 mg, 0.592 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (183.0 mg, 50%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.76-8.32 (m, 2H, TFA), 7.95 (br s, 1H), 7.93-7.87 (m, 2H), 7.86-7.81 (m, 1H), 7.64 (d, J=10.3 Hz, 1H), 7.49-7.43 (m, 2H), 7.20 (d, J=8.7 Hz, 1H), 6.35 (s, 2H), 3.43-3.38 (m, 2H), 3.26-3.19 (m, 2H), 3.18-3.11 (m, 2H), 2.96 (s, 4H), 2.16-2.09 (m, 2H), 1.93-1.82 (m, 2H); LCMS: [M+H]+=473.30.
A procedure analogous to Example 144, Step 3 using tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)spiro[chromane-2,4′-piperidine]-1′-carboxylate (339 mg, 0.607 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (225.3 mg, 77%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.92 (m, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.57 (d, J=10.1 Hz, 1H), 7.46 (dd, J=1.5, 7.9 Hz, 1H), 7.44 (s, 1H), 7.26-7.21 (m, 2H), 6.79 (d, J=8.8 Hz, 1H), 6.19 (s, 2H), 3.40 (td, J=3.2, 6.5 Hz, 2H), 3.17 (s, 1H), 2.95 (t, J=6.5 Hz, 2H), 2.82 (br s, 2H), 2.74 (s, 2H), 2.70 (br s, 2H), 1.77 (br t, J=6.7 Hz, 2H), 1.61 (br s, 2H), 1.50 (br s, 2H); LCMS: [M+H]+=459.38.
A procedure analogous to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-cyanophenoxy)piperidine-1-carboxylate (320 mg, 0.574 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 afforded the title compound (159.1 mg, 58%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.89 (m, 3H), 7.80 (br d, J=8.9 Hz, 1H), 7.70 (d, J=10.3 Hz, 1H), 7.51-7.47 (m, 1H), 7.45 (s, 1H), 7.35 (d, J=9.0 Hz, 1H), 6.37 (s, 2H), 4.73-4.64 (m, 1H), 3.44-3.38 (m, 2H), 3.01-2.91 (m, 4H), 2.64-2.56 (m, 2H), 2.34-2.03 (m, 1H), 1.97-1.87 (m, 2H), 1.59-1.49 (m, 2H); LCMS: [M+H]+=458.18.
A procedure analogous to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate (115.0 mg, 0.196 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 afforded the title compound (70.2 mg, 70%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.83 (br d, J=8.4 Hz, 1H), 7.79 (s, 1H), 7.72 (d, J=10.1 Hz, 1H), 7.55 (d, J=8.4 Hz, 1H), 7.51-7.47 (m, 1H), 7.46 (s, 1H), 6.40 (s, 2H), 3.45-3.38 (m, 2H), 2.99-2.93 (m, 2H), 2.82 (br d, J=5.4 Hz, 8H); LCMS: [M+H]+=486.32.
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (23.9 mg, 0.055 mmol) and N,N-diisopropylethylamine (0.096 mL, 0.553 mmol) in DMF (1.5 mL) was added 2,2,2-trifluoroethyl trifluoromethanesulfonate (22.3 mg, 0.096 mmol) at RT. The reaction mixture was stirred for 3 hours at RT, then passed through a PoraPak Rxn CX (15cc-2g) cartridge in a catch & elute method by first loading the solution of product in MeOH onto the MeOH-rinsed cartridge and releasing product with 20 mL of a solution of ammonia in MeOH (3N) to afford the crude product. Further purification using flash silica gel chromatography (0-10% MeOH/DCM) to afforded the product (25.8 mg, 86%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 4H), 7.00 (d, J=8.9 Hz, 2H), 6.22 (s, 2H), 4.47-4.39 (m, 1H), 3.43-3.37 (m, 2H), 3.19 (q, J=10.2 Hz, 2H), 2.95 (s, 2H), 2.90-2.82 (m, 2H), 2.62-2.54 (m, 2H), 1.98-1.88 (m, 2H), 1.71-1.59 (m, 2H); LCMS: [M+H]+=515.51.
A procedure similar to Example 186 was used with 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (22.3 mg, 0.052 mmol), N,N-diisopropylethylamine (0.090 ml, 0.516 mmol) in DMF(1.5 mL) and addition 1,1,1-trifluoro-3-iodopropane (6.04 μl, 0.052 mmol) at RT. After stirring the reaction mixture for 72 h at RT additional 1,1,1-trifluoro-3-iodopropane (6.04 μl, 0.052 mmol) was added and the mixture was stirred 3 h at RT, then heated at 50° C. for 16 h. A workup and purification similar to Example 186 afforded the title compound (11.9 mg, 42%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.48-7.43 (m, 4H), 6.99 (d, J=8.8 Hz, 2H), 6.22 (s, 2H), 4.45-4.37 (m, 1H), 3.43-3.38 (m, 2H), 2.98-2.92 (m, 2H), 2.76-2.67 (m, 2H), 2.56-2.51 (m, 2H), 2.48-2.40 (m, 2H), 2.32-2.23 (m, 2H), 1.98-1.90 (m, 2H), 1.67-1.58 (m, 2H); LCMS: [M+H]+=529.43.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (22.3 mg, 0.052 mmol) and N,N-diisopropylethylamine (0.090 ml, 0.516 mmol) in DMF (1.5 mL) and 1-bromo-4,4,4-trifluorobutane (17 mg, 0.089 mmol) afforded the title compound (24.7 mg, 84%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 4H), 6.99 (d, J=8.8 Hz, 2H), 6.22 (s, 2H), 4.44-4.36 (m, 1H), 3.43-3.37 (m, 2H), 2.98-2.92 (m, 2H), 2.72-2.62 (m, 2H), 2.39-2.33 (m, 2H), 2.30-2.18 (m, 4H), 1.98-1.90 (m, 2H), 1.67-1.58 (m, 4H); LCMS: [M+H]+=543.35.
A procedure similar to Example 186 with 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (27.4 mg, 0.063 mmol) and N,N-diisopropylethylamine (0.110 ml, 0.634 mmol) in DMF (1.5 ml) and adding 2-bromoethyl methyl ether (5.95 μl, 0.063 mmol) at RT was used. After stirring for 72 h at RT, additional 2-bromoethyl methyl ether (5.95 μl, 0.063 mmol) was added and the mixture was stirred for 3 h at RT. A similar workup and isolation procedure as Example 186 afforded the title compound (20.0 mg, 61%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 4H), 6.99 (br d, J=8.7 Hz, 2H), 6.22 (s, 2H), 4.51-4.31 (m, 1H), 3.50-3.43 (m, 2H), 3.40 (br d, J=2.4 Hz, 2H), 3.24 (s, 3H), 2.95 (br t, J=6.5 Hz, 2H), 2.88-2.65 (m, 2H), 2.63-2.51 (m, 2H), 2.42-2.14 (m, 2H), 2.05-1.88 (m, 2H), 1.73-1.56 (m, 2H); LCMS: [M+H]+=491.51.
Step 1: tert-butyl 4-(4-bromo-2-(trifluoromethyl)phenoxy)piperidine-1-carboxylate
A procedure similar to Example 147, Step 1 using 4-bromo-2-trifluoromethylphenol (765 mg, 3.17 mmol), tert-butyl 4-hydroxy-1-piperidinecarboxylate (638.9 mg, 3.17 mmol), PPh3 (916 mg, 3.49 mmol) in THF (10 ml) and diisopropyl azodicarboxylate (0.750 ml, 3.81 mmol) afforded the product (0.7131 g, 46%) and 4-bromo-2-(trifluoromethyl)phenol with a ratio of 84:16 and as a colorless oil which was used for the next step. LCMS: [M−Me+H]+=411.30.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(trifluoromethyl)phenoxy)piperidine-1-carboxylate
A procedure analogous to Example 43, Step 5 using tert-butyl 4-(4-bromo-2-(trifluoromethyl)phenoxy)piperidine-1-carboxylate (350 mg, 0.693 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (345 mg, 0.901 mmol), 1,4-dioxane (4 mL), K3PO4 (441 mg, 2.08 mmol) in water (1 mL) and XPhos Pd G2 (54.5 mg, 0.069 mmol) afforded the title compound (330.5 mg, 79%) as a beige powder. LCMS: [M+H]+=601.50.
Step 3: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(trifluoromethyl)phenoxy)piperidine-1-carboxylate (330.5 mg, 0.550 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (178.9 mg, 62%) as a slightly yellow powder which will be used for the next step. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.77-7.70 (m, 2H), 7.68 (br d, J=10.0 Hz, 1H), 7.47 (br d, J=7.9 Hz, 1H), 7.44 (s, 1H), 7.35 (br d, J=8.7 Hz, 1H), 6.33 (s, 2H), 4.72-4.64 (m, 1H), 3.43-3.37 (m, 2H), 2.98-2.87 (m, 4H), 2.63-2.55 (m, 2H), 1.92-1.85 (m, 2H), 1.57-1.47 (m, 2H); LCMS: [M+H]+=501.36.
To a solution of 6-(2-amino-6-fluoro-5-(4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24.4 mg, 0.053 mmol) and N,N-diisopropylethylamine (0.046 ml, 0.265 mmol) in CH2Cl2 (3 mL) was added acetic anhydride (5.01 μl, 0.053 mmol) at RT. The reaction mixture was stirred for 1 h at 23° C. The solvents were removed and the crude mixture was dissolved in MeOH and loaded onto a MeOH washed PoraPak Rxn CX (15cc-2g) cartridge. After washing the cartridge was washed with additional MeOH (3×20 mL), the product was released using 20 mL of a solution of ammonia in MeOH (3N) to afford, after removal of solvent, the title compound (25.9 mg, 92%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.48-7.45 (m, 1H), 7.44 (s, 1H), 7.40-7.35 (m, 1H), 7.29 (s, 1H), 6.90 (d, J=8.6 Hz, 1H), 6.25 (s, 2H), 4.89 (s, 2H), 3.61-3.55 (m, 1H), 3.54-3.48 (m, 3H), 3.42-3.38 (m, 2H), 2.95 (s, 2H), 2.03 (s, 3H), 1.89 (br s, 2H), 1.82-1.74 (m, 2H); LCMS: [M+H]+=503.47.
A procedure analogous to Example 191 using 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)spiro[chromane-2,4′-piperidin]-4-one (23.7 mg, 0.050 mmol), N,N-diisopropylethylamine (0.044 ml, 0.251 mmol) in CH2Cl2 (3 mL) and acetic anhydride (4.74 μl, 0.050 mmol) afforded the title compound (18.6 mg, 68.5% yield) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.87 (s, 1H), 7.83-7.77 (m, 1H), 7.65 (d, J=10.3 Hz, 1H), 7.50-7.46 (m, 1H), 7.45 (s, 1H), 7.15 (d, J=8.7 Hz, 1H), 6.33 (s, 2H), 4.16-4.07 (m, 1H), 3.70-3.61 (m, 1H), 3.40 (dt, J=2.4, 6.5 Hz, 3H), 2.95 (br t, J=6.6 Hz, 3H), 2.87 (s, 2H), 2.01 (s, 3H), 1.98-1.87 (m, 2H), 1.79-1.70 (m, 1H), 1.63-1.55 (m, 1H); LCMS: [M+H]+=515.51.
A procedure analogous to Example 191 using 6-(2-amino-6-fluoro-5-(spiro[chromane-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (25.9 mg, 0.056 mmol) and N,N-diisopropylethylamine (0.049 ml, 0.282 mmol) in CH2Cl2 (3 mL) and acetic anhydride (5.34 μl, 0.056 mmol) afforded the title compound (28.0 mg, 94%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.46 (d, J=7.9 Hz, 1H), 7.44 (s, 1H), 7.28 (s, 2H), 6.84 (d, J=8.2 Hz, 1H), 6.20 (s, 2H), 4.13-4.04 (m, 1H), 3.69-3.59 (m, 1H), 3.44-3.37 (m, 3H), 3.06-2.98 (m, 1H), 2.95 (s, 2H), 2.77 (s, 2H), 2.02 (s, 3H), 1.81 (s, 2H), 1.78-1.59 (m, 3H), 1.54-1.44 (m, 1H); LCMS: [M+H]+=501.51.
A procedure analogous to Example 191 using 5-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (27.1 mg, 0.059 mmol) and N,N-diisopropylethylamine (0.052 mL, 0.296 mmol) in CH2Cl2 (3 mL) and acetic anhydride (5.60 μl, 0.059 mmol) afforded the title compound (29.4 mg, 94%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (d, J=2.0 Hz, 2H), 7.91 (d, J=7.9 Hz, 1H), 7.83 (br d, J=8.9 Hz, 1H), 7.71 (d, J=10.3 Hz, 1H), 7.48 (dd, J=8.1, 1.5 Hz, 1H), 7.46 (s, 1H), 7.41 (d, J=9.0 Hz, 1H), 6.38 (s, 2H), 4.95-4.85 (m, 1H), 3.73-3.60 (m, 2H), 3.46-3.36 (m, 4H), 2.99-2.91 (m, 2H), 2.03 (s, 3H), 2.01-1.94 (m, 1H), 1.93-1.85 (m, 1H), 1.78-1.68 (m, 1H), 1.65-1.56 (m, 1H); LCMS: [M+H]+=500.46.
A procedure analogous to Example 186 using 6-(2-amino-6-fluoro-5-(4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (21.8 mg, 0.047 mmol), N,N-diisopropylethylamine (0.082 mL, 0.473 mmol) in DMF (1 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (21.97 mg, 0.095 mmol) afforded the title compound (27.2 mg, 101% yield) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.46 (d, J=7.9 Hz, 1H), 7.44 (s, 1H), 7.36 (br d, J=8.6 Hz, 1H), 7.27 (s, 1H), 6.88 (d, J=8.6 Hz, 1H), 6.24 (s, 2H), 4.89-4.82 (m, 2H), 3.44-3.37 (m, 2H), 3.25 (q, J=10.4 Hz, 2H), 2.95 (s, 2H), 2.73 (br s, 4H), 1.91-1.82 (m, 4H); LCMS: [M+H]+=543.50.
A procedure similar to Example 186 using 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)spiro[chromane-2,4′-piperidin]-4-one (26.1 mg, 0.055 mmol) and N,N-diisopropylethylamine (0.096 mL, 0.552 mmol) in DMF (1 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (25.6 mg, 0.110 mmol) afforded the title compound (17.4 mg, 54%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.86 (s, 1H), 7.80-7.76 (m, 1H), 7.64 (d, J=10.3 Hz, 1H), 7.50-7.43 (m, 2H), 7.11 (d, J=8.6 Hz, 1H), 6.33 (s, 2H), 3.40 (dt, J=2.7, 6.5 Hz, 2H), 3.20 (q, J =10.3 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.84 (s, 2H), 2.77-2.67 (m, 4H), 1.91 (br d, J=13.0 Hz, 2H), 1.80-1.70 (m, 2H); LCMS: [M+H]+=555.53.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(spiro[chromane-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (23.3 mg, 0.051 mmol), N,N-diisopropylethylamine (0.089 mL, 0.508 mmol) in DMF (1 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (23.59 mg, 0.102 mmol) afforded the title compound (24.0 mg, 83%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.49-7.44 (m, 1H), 7.44 (s, 1H), 7.26 (s, 2H), 6.80 (d, J=8.2 Hz, 1H), 6.20 (s, 2H), 3.44-3.37 (m, 2H), 3.19 (q, J=10.2 Hz, 2H), 2.95 (s, 2H), 2.79-2.68 (m, 6H), 1.78 (s, 2H), 1.74-1.59 (m, 4H); LCMS: [M+H]+=541.46.
A procedure similar to Example 186 using 5-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (22.4 mg, 0.049 mmol), N,N-diisopropylethylamine (0.085 mL, 0.490 mmol) in DMF (1 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (22.73 mg, 0.098 mmol) afforded the title compound (27.0 mg, 97%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.98-7.88 (m, 3H), 7.81 (br d, J=9.0 Hz, 1H), 7.71 (d, J=10.3 Hz, 1H), 7.51-7.47 (m, 1H), 7.45 (s, 1H), 7.36 (d, J=9.2 Hz, 1H), 6.37 (s, 2H), 4.74-4.66 (m, 1H), 3.44-3.38 (m, 2H), 3.21 (q, J=10.1 Hz, 2H), 2.98-2.93 (m, 2H), 2.90-2.83 (m, 2H), 2.68-2.60 (m, 2H), 2.01-1.91 (m, 2H), 1.77-1.68 (m, 2H); LCMS: [M+H]+=540.49.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (23.0 mg, 0.046 mmol), N,N-diisopropylethylamine (0.040 ml, 0.230 mmol) in CH2Cl2 (3 mL) and acetic anhydride (4.34 μl, 0.046 mmol) afforded the title compound (25.8 mg, 98%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.79 (br d, J=8.8 Hz, 1H), 7.75 (s, 1H), 7.69 (d, J=10.1 Hz, 1H), 7.50-7.46 (m, 1H), 7.45 (s, 1H), 7.40 (d, J=8.9 Hz, 1H), 6.34 (s, 2H), 4.95-4.87 (m, 1H), 3.61-3.50 (m, 3H), 3.48-3.43 (m, 1H), 3.42-3.38 (m, 2H), 2.95 (s, 2H), 2.02 (s, 3H), 2.00-1.92 (m, 1H), 1.90-1.82 (m, 1H), 1.76-1.68 (m, 1H), 1.65-1.57 (m, 1H); LCMS: [M+H]+=543.57.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (20.8 mg, 0.043 mmol), N,N-diisopropylethylamine (0.037 mL, 0.214 mmol) in CH2Cl2 (3 mL) and acetic anhydride (4.05 μl, 0.043 mmol) afforded the title compound (22.8 mg, 96%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.84 (br d, J=8.4 Hz, 1H), 7.81 (s, 1H), 7.73 (d, J=10.1 Hz, 1H), 7.60 (d, J=8.3 Hz, 1H), 7.48 (br d, J=8.1 Hz, 1H), 7.45 (s, 1H), 6.42 (s, 2H), 3.56 (br d, J=3.8 Hz, 4H), 3.44-3.37 (m, 2H), 2.95 (s, 2H), 2.89 (br s, 2H), 2.83 (br s, 2H), 2.04 (s, 3H); LCMS: [M+H]+=528.52
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (22.1 mg, 0.044 mmol), N,N-diisopropylethylamine (0.077 ml, 0.442 mmol) in DMF (1 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (20.50 mg, 0.088 mmol) afforded the title compound (19.6 mg, 72%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.76 (br d, J=8.9 Hz, 1H), 7.74 (s, 1H), 7.68 (d, J=10.3 Hz, 1H), 7.47 (dd, J=1.4, 8.0 Hz, 1H), 7.45 (s, 1H), 7.36 (d, J=8.8 Hz, 1H), 6.34 (s, 2H), 4.75-4.67 (m, 1H), 3.45-3.37 (m, 2H), 3.19 (q, J=10.2 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.87-2.77 (m, 2H), 2.63 (dt, J=3.7, 7.5 Hz, 2H), 1.98-1.88 (m, 2H), 1.77-1.67 (m, 2H); LCMS: [M+H]+=583.52.
A procedure analogous to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (20.1 mg, 0.041 mmol), N,N-diisopropylethylamine (0.072 mL, 0.414 mmol) in DMF (1 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (19.22 mg, 0.083 mmol) afforded the title compound (15.7 mg, 64%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.82 (br d, J=8.7 Hz, 1H), 7.79 (s, 1H), 7.72 (d, J=10.1 Hz, 1H), 7.63 (d, J=8.4 Hz, 1H), 7.48 (dd, J=1.6, 7.9 Hz, 1H), 7.45 (s, 1H), 6.41 (s, 2H), 3.44-3.38 (m, 2H), 3.25 (q, J=10.2 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.92-2.87 (m, 4H), 2.77 (br s, 4H); LCMS: [M+H]+=568.55.
A procedure analogous to Example 191, using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (23.7 mg, 0.055 mmol) and cyclopropanecarbonyl chloride (5.02 μl, 0.055 mmol) in CH2Cl2 (2 mL) and N,N-diisopropylethylamine (0.095 mL, 0.548 mmol), afforded the title compound (22.0 mg, 76%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.92 (m, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.3 Hz, 1H), 7.47 (br d, J=8.3 Hz, 4H), 7.04 (d, J=8.8 Hz, 2H), 6.23 (s, 2H), 4.71-4.63 (m, 1H), 4.04-3.92 (m, 1H), 3.87 (br d, J=5.1 Hz, 1H), 3.54 (br s, 1H), 3.40 (dt, J=6.5, 2.7 Hz, 2H), 3.29-3.20 (m, 1H), 2.95 (t, J=6.5 Hz, 2H), 2.08-1.96 (m, 2H), 1.96-1.85 (m, 1H), 1.63 (br d, J=2.1 Hz, 1H), 1.52 (br s, 1H), 0.77-0.65 (m, 4H); LCMS: [M+H]+=501.51.
Step 1: 3-chloro-5-(3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)-6-fluoropyridin-2-amine
A procedure analogous to Example 177, Step 1 using 3,4-dihydro-2H-1,5-benzodioxepin-7-ylboronic acid (177 mg, 0.910 mmol), 5-bromo-3-chloro-6-fluoropyridin-2-amine (205.2 mg, 0.910 mmol), 1,4-dioxane (4 mL) and Cs2CO3 (890 mg, 2.73 mmol) in water (1 mL) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (100 mg, 0.137 mmol) afforded the product (178.1 mg, 66%) as a white powder. LCMS: [M+H]+=295.19.
Step 2: 6-(2-amino-5-(3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 43, Step 5 using 3-chloro-5-(3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)-6-fluoropyridin-2-amine (52 mg, 0.176 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (53.0 mg, 0.194 mmol), 1,4-dioxane (3 mL), K3PO4 (112 mg, 0.529 mmol) in water (1 mL) and XPhos Pd G2 (13.88 mg, 0.018 mmol) afforded the title compound (31.0 mg, 41%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.50-7.43 (m, 2H), 7.16 (s, 1H), 7.13 (br d, J=8.4 Hz, 1H), 6.99 (d, J=8.3 Hz, 1H), 6.28 (s, 2H), 4.18-4.11 (m, 4H), 3.43-3.38 (m, 2H), 2.98-2.92 (m, 2H), 2.15-2.07 (m, 2H); LCMS: [M+H]+=406.41.
A procedure analogous to Example 43, Step 5 using 3-chloro-5-(3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)-6-fluoropyridin-2-amine (51.6 mg, 0.175 mmol), 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (56.1 mg, 0.193 mmol), 1,4-dioxane (3 mL), K3PO4 (111 mg, 0.525 mmol) in water (1 mL) and XPhos Pd G2 (13.78 mg, 0.018 mmol) afforded the title compound (30.5 mg, 39%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (brs, 1H), 7.60 (dd, J=10.1, 3.0 Hz, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.16-7.07 (m, 2H), 6.99 (d, J=8.3 Hz, 1H), 6.28 (s, 2H), 4.18-4.10 (m, 4H), 3.44-3.38 (m, 2H), 2.95-2.89 (m, 2H), 2.15-2.07 (m, 2H); LCMS: [M+H]+=424.39.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.261 mmol), 1-[(4-bromobenzene)sulfonyl]-4-methylhomopiperazine (109 mg, 0.326 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (15.91 mg, 0.022 mmol), K3PO4 (0.335 mL of a 1.3 M aqueous solution, 0.435 mmol), DME (4 mL) and H2O (2 mL) afforded the title compound (50 mg, 48%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.82-7.77 (m, 4H), 7.75 (d, J=10.1 Hz, 1H), 7.48 (d, J=7.9 Hz, 1H), 7.46 (s, 1H), 6.52 (s, 2H), 3.42-3.39 (m, 3H), 3.31-3.27 (m, 4H), 2.55-2.52 (m, 2H), 2.49-2.45 (m, 2H), 2.22 (s, 4H), 1.73 (quin, J=5.7 Hz, 2H); LCMS: [M+1]+=510.39.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (115 mg, 0.300 mmol), 1-(4-bromophenylsulfonyl)-4-methylpiperazine (120 mg, 0.375 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.30 mg, 0.025 mmol), aqueous K3PO4 (0.385 mL of a 1.3 M solution, 0.500 mmol), DME (4 mL) and H2O (2 mL) afforded the title compound (0.108 mmol, 43%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.87-7.83 (m, 2H), 7.79-7.71 (m, 3H), 7.51-7.44 (m, 2H), 6.54 (br s, 2H), 3.40 (br d, J=6.8 Hz, 2H), 2.97-2.93 (m, 2H), 2.90 (br s, 4H), 2.36 (br s, 4H), 2.13 (s, 3H); LCMS: [M+1]+=496.47.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-4-methoxypiperidine-1-carboxylate
A procedure analogous to that of Example 43, Step 1 heating tert-butyl 4-(4-chlorophenyl)-4-methoxypiperidine-1-carboxylate (59.5 mg, 0.164 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (88 mg, 0.230 mmol) at 85° C. for 1.75 h afforded the title compound as a white powder (55 mg, 56% yield based on purity of 92%). LCMS: [M+H]+=547.70.
Step 2: 6-(2-amino-6-fluoro-5-(4-(4-methoxy-1-(2-methoxyethyl)piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A solution of 4.0 M HCl in dioxane (0.629 mL, 2.52 mmol) was added to a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-4-methoxypiperidine-1-carboxylate (55 mg, 0.101 mmol) in MeOH (4 mL). The reaction mixture was stirred at RT for 2.5 h and concentrated onto Celite®. Reverse phase chromatography (C18; 0-20% acetonitrile/water) followed by filtration through a Waters PoraPak CX column afforded 56 mg of the intermediate 6-(2-amino-6-fluoro-5-(4-(4-methoxypiperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one as a beige solid. This intermediate was taken up in DMF (3 mL) with 2-bromoethyl methyl ether (0.047 mL, 0.503 mmol) and Et3N (0.210 ml, 1.51 mmol) and stirred overnight at RT. The reaction mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18; 0-40% acetonitrile/water) to afford the title compound as a beige powder (27 mg, 51%). 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.94 (m, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.71-7.66 (m, 1H), 7.58 (br d, J=7.6 Hz, 2H), 7.51-7.43 (m, 3H), 6.38-6.30 (m, 2H), 3.56-3.51 (m, 4H), 3.44-3.41 (m, 5H), 3.28 (br s, 3H), 2.99-2.89 (m, 5H), 2.89-2.89 (m, 1H), 2.19-1.77 (m, 4H); LCMS: [M+H]+=505.65.
Step 1: 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A mixture of 5-bromo-6-chloro-3-iodopyridin-2-amine (500 mg, 1.50 mmol), 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (570 mg, 1.70 mmol, 87% purity) Na2CO3—H2O (391 mg, 3.15 mmol) and bis(triphenylphosphine)palladium(II) dichloride (126 mg, 0.180 mmol), in CH3CN (28 mL) and H2O (12 mL) was heated in a microwave apparatus at 80° C. for 4 h. The reaction mixture was partitioned between brine and EtOAc. The organic phase was separated and aqueous phase was further extracted with DCM (2×10 mL). The combined organic layers were combined, dried over Na2SO4 and concentrated onto Celite®. Silica gel chromatography (eluting with 0-100% EtOAc/Hexanes) afforded the product as a beige solid (314 mg, 57%); LCMS: [M+H]+=372.33.
Step 2: 6-(2-Amino-6-chloro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 45, step 5 using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.135 mmol) and 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (62.4 mg, 0.189 mmol) at 90° C. for 1.75 h afforded the title compound as a beige powder (30 mg, 43%). 1H NMR (500 MHz, DMSO-d6) δ 8.20-8.05 (m, 1H), 7.68-7.56 (m, 1H), 7.48-7.39 (m, 1H), 7.39-7.37 (m, 1H), 7.39-7.24 (m, 2H), 6.96 (br d, J=6.5 Hz, 2H), 6.35-6.15 (m, 2H), 3.49-3.38 (m, 2H), 3.21-3.05 (m, 4H), 2.98-2.88 (m, 2H), 2.76-2.66 (m, 1H), 2.64-2.54 (m, 4H), 1.11-0.97 (m, 6H). LCMS: [M+H]+=494.55.
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one-TFA (40 mg, 0.075 mmol) and N,N-diisopropylethylamine (0.131 ml, 0.753 mmol) in DMF (1.0 mL) was added (bromomethyl)cyclopropane (15.24 mg, 0.113 mmol). The reaction was stirred at 70° C. for 4 h. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the title compound (13.6 mg, 0.029 mmol, 38%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.83 (br s, 1H), 7.79 (d, J=7.8 Hz, 1H), 7.47 (d, J=10.2 Hz, 1H), 7.36 (br d, J=8.1 Hz, 1H), 7.34 (s, 1H), 7.29 (br d, J=8.1 Hz, 2H), 6.87 (br d, J=8.7 Hz, 2H), 6.07 (s, 2H), 3.28-3.31 (m, 2H), 3.06 (br d, J=5.3 Hz, 4H), 2.84 (br t, J=6.4 Hz, 2H), 2.48 (br s, 2H), 2.13 (br s, 2H), 0.75 (br d, J=6.6 Hz, 1H), 0.38 (br d, J=6.9 Hz, 2H), 0.00 (br s, 2H); LCMS: [M+H]+=472.55.
To a suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.113 mmol), 1-ethyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (42.9 mg, 0.136 mmol) and Cs2CO3 (92 mg, 0.282 mmol) in a 3:1 mixture of DME (6 mL): water (2 mL) was added PdCl2dppf (8.26 mg, 0.011 mmol). The mixture was flushed with nitrogen then heated in the microwave at 90° C. for 2 hours. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM, followed by reverse phase chromatography (C18, 0-100% ACN/H2O) to afford the title compound (9.7 mg, 19%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.60 (dd, J=13.0, 10.2 Hz, 2H), 7.43 (d, J=7.0 Hz, 1H), 7.38 (d, J=7.8 Hz, 2H), 6.97 (d, J=8.9 Hz, 2H), 6.19 (s, 2H), 3.41 (td, J=6.5, 2.8 Hz, 2H), 3.14-3.20 (m, 4H), 2.92 (br t, J=6.5 Hz, 2H), 2.37 (q, J=7.2 Hz, 2H), 1.04 (t, J=7.2 Hz, 3H); LCMS: [M+H]+=464.47.
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one-TFA (40 mg, 0.073 mmol) and N,N-diisopropylethylamine (0.127 ml, 0.728 mmol) in DMF (1.0 mL) was added 2-bromoethyl methyl ether (15.18 mg, 0.109 mmol). The reaction was stirred at 70° C. for 2 h. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the title compound (16 mg, 45%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.04 (br s, 1H), 7.52 (dd, J=12.5, 10.3 Hz, 2H), 7.35 (d, J=7.1 Hz, 1H), 7.31 (br d, J=8.0 Hz, 2H), 6.90 (br d, J=7.8 Hz, 2H), 6.12 (br s, 2H), 3.41 (br d, J=2.5 Hz, 2H), 3.33 (br dd, J=6.5, 2.5 Hz, 2H), 3.16-3.21 (m, 3H), 3.07 (br s, 4H), 2.85 (br t, J=6.4 Hz, 2H), 2.49 (br s, 2H); LCMS: [M+H]+=494.55.
A procedure similar to Example 212 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one-TFA (25 mg, 0.045 mmol) and N,N-diisopropylethylamine (0.079 mL, 0.455 mmol) in DMF (1.0 mL) and (bromomethyl)cyclopropane (9.21 mg, 0.068 mmol) afforded the title compound (5.4 mg, 24%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.00 (br s, 1H), 7.49 (dd, J=12.8, 10.2 Hz, 2H), 7.32 (d, J=7.1 Hz, 1H), 7.28 (d, J=7.8 Hz, 2H), 6.87 (d, J=8.9 Hz, 2H), 6.08 (s, 2H), 3.29-3.31 (m, 2H), 3.04-3.09 (m, 4H), 2.82 (br t, J=6.4 Hz, 2H), 2.46-2.50 (m, 4H), 2.13 (br d, J=6.6 Hz, 2H), 0.73-0.78 (m, 1H), 0.35-0.41 (m, 2H),−0.03-0.02 (m, 2H); LCMS: [M+H]+=490.67.
A procedure analogous to Example 186 using 6-(2-amino-6-fluoro-5-(4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (29.2 mg, 0.063 mmol), N,N-diisopropylethylamine (0.110 mL, 0.634 mmol) DMF (1 mL) and 2-bromoethyl methyl ether (0.018 ml, 0.190 mmol) afforded the title compound (31.0 mg, 85%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 2H), 7.35 (br d, J=8.6 Hz, 1H), 7.26 (s, 1H), 6.87 (d, J=8.6 Hz, 1H), 6.24 (s, 2H), 4.85 (s, 2H), 3.46-3.38 (m, 5H), 3.23 (s, 3H), 2.95 (br t, J=6.5 Hz, 2H), 2.52 (br s, 4H), 1.89-1.78 (m, 4H); LCMS: [M+H]+=519.57.
A procedure analogous to Example 186 using 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)spiro[chromane-2,4′-piperidin]-4-one (26.1 mg, 0.055 mmol) and N,N-diisopropylethylamine (0.096 ml, 0.552 mmol), DMF (1 mL) and 2-bromoethyl methyl ether (0.016 ml, 0.166 mmol) afforded the title compound (23.1 mg, 73%) as a slightly yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.85 (s, 1H), 7.80-7.75 (m, 1H), 7.64 (d, J=10.3 Hz, 1H), 7.50-7.46 (m, 1H), 7.45 (s, 1H), 7.10 (d, J=8.7 Hz, 1H), 6.33 (s, 2H), 3.46-3.38 (m, 5H), 3.22 (s, 3H), 2.95 (br t, J=6.5 Hz, 2H), 2.82 (s, 2H), 2.65-2.57 (m, 2H), 2.38 (br t, J=10.6 Hz, 2H), 1.94-1.85 (m, 2H), 1.76-1.66 (m, 2H); LCMS: [M+H]+=531.38.
A procedure similar to Example 186, using 6-(2-amino-6-fluoro-5-(spiro[chromane-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (28.3 mg, 0.062 mmol), N,N-diisopropylethylamine (0.108 mL, 0.617 mmol), DMF (1 mL) and 2-bromoethyl methyl ether (0.017 mL, 0.185 mmol) afforded the title compound (30.0 mg, 88%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.57 (d, J=10.1 Hz, 1H), 7.48-7.45 (m, 1H), 7.44 (s, 1H), 7.27-7.21 (m, 2H), 6.79 (d, J=8.3 Hz, 1H), 6.19 (s, 2H), 3.42 (br d, J=6.0 Hz, 5H), 3.23 (s, 3H), 2.95 (t, J=6.5 Hz, 2H), 2.74 (br t, J=6.8 Hz, 2H), 2.63-2.55 (m, 2H), 2.39 (br t, J=9.7 Hz, 2H), 1.77 (br t, J=6.7 Hz, 2H), 1.73-1.66 (m, 2H), 1.65-1.55 (m, 2H); LCMS: [M+H]+=517.31.n
A procedure analogous to Example 186 using 5-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (29.5 mg, 0.064 mmol) and N,N-diisopropylethylamine (0.112 mL, 0.645 mmol), DMF (1 mL) and 2-bromoethyl methyl ether (0.018 mL, 0.193 mmol) afforded the title compound (32.1 mg, 91%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.92 (d, J=2.2 Hz, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.81 (br d, J=9.2 Hz, 1H), 7.71 (d, J=10.3 Hz, 1H), 7.48 (dd, J=7.9, 1.5 Hz, 1H), 7.45 (s, 1H), 7.35 (d, J=9.2 Hz, 1H), 6.37 (s, 2H), 4.70-4.62 (m, 1H), 3.46-3.38 (m, 5H), 3.23 (s, 3H), 2.95 (t, J=6.5 Hz, 2H), 2.72-2.63 (m, 2H), 2.40-2.32 (m, 2H), 1.99-1.89 (m, 2H), 1.74-1.65 (m, 2H); LCMS: [M−H]−=514.53.
To a solution of 6-(2-amino-6-fluoro-5-(4H-spiro[benzo[d][1,3]dioxine-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (26.0 mg, 0.056 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL) was added 2 drops of glacial AcOH (24 mg, 0.400 mmol) at RT and the reaction mixture was stirred for 30 min. Then NaBH(OAc)3 (50 mg, 0.236 mmol) was added and the reaction mixture was stirred at 23° C. for 16 h. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-20% DCM/MeOH) to afford the title compound (22.3 mg, 75%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.46 (d, J=8.1 Hz, 1H), 7.44 (s, 1H), 7.35 (br d, J=8.6 Hz, 1H), 7.26 (s, 1H), 6.87 (d, J=8.6 Hz, 1H), 6.24 (s, 2H), 4.84 (s, 2H), 3.41-3.38 (m, 4H), 2.99-2.92 (m, 2H), 2.74 (td, J=13.0, 6.4 Hz, 1H), 2.57-2.51 (m, 2H), 1.88-1.76 (m, 4H), 0.98 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=503.55.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 4-(4-(6-amino-5-chloro-2-fluoropyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate (152 mg, 0.320 mmol), 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (112 mg, 0.384 mmol), 1,4-dioxane (4 mL), K3PO4 (204 mg, 0.960 mmol) in water (1 mL) and XPhos Pd G2 (25.2 mg, 0.032 mmol) afforded the product (114.4 mg, 56%) as a dark beige powder which was used for the next step. LCMS: [M+H]+=604.51.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate (114.4 mg, 0.190 mmol), TFA (2 mL, 26.1 mmol) and CH2Cl2 (4 mL) afforded the title compound (71.7 mg, 71%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.80 (br d, J=8.6 Hz, 1H), 7.76 (s, 1H), 7.73 (d, J=10.3 Hz, 1H), 7.61 (d, J=10.0 Hz, 1H), 7.53 (d, J=8.4 Hz, 1H), 7.43 (d, J=7.1 Hz, 1H), 6.41 (s, 2H), 3.40 (dt, J=6.5, 2.7 Hz, 2H), 2.92 (br t, J=6.4 Hz, 2H), 2.80 (br d, J=4.6 Hz, 8H), NH not shown; LCMS: [M+H]+=504.45.
Step 1: 6-(5-(4-((1R,5S)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A by heating a sealed, degassed mixture of (1R,5S)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (300 mg, 1.26 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (579 mg, 1.51 mmol), PdCl2dppf (92 mg, 0.13 mmol), Cs2CO3 (1231 mg, 3.78 mmol), DME (10 mL) and H2O (5 mL) in a microwave reactor at 90° C. for 2 h afforded the title compound as a tan solid (298 mg, 57%). LCMS: [M+H]+=415.44.
Step 2: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-(2-methoxyacetyl)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a CH2Cl2 (15 mL) solution of 6-(5-(4-((1R,5S)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (60 mg, 0.14 mmol) and i-Pr2NEt (0.063 mL, 0.36 mmol) was added a CH2Cl2 solution of methoxyacetyl chloride (22.9 mg/mL in CH2Cl2, 0.68 mL, 0.14 mmol) at 0° C. and the reaction was allowed to slowly warm to RT and then kept stirred overnight. An excess of 2 M NH3/MeOH was added and after briefly stirring at RT, the reaction was concentrated under reduced pressure and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2) to afford the title compound (63 mg, 89%) as a light beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.64 (d, J=10.0 Hz, 1H), 7.41-7.56 (m, 4H), 7.28 (d, J=7.2 Hz, 2H), 6.32 (s, 2H), 4.00-4.13 (m, 2H), 3.94-4.00 (m, 1H), 3.66-3.82 (m, 1H), 3.64-3.66 (m, 1H), 3.38-3.57 (m, 3H), 3.29-3.32 (m, 3H), 2.96 (br t, J=6.5 Hz, 2H), 1.94-2.13 (m, 1H), 1.07-1.24 (m, 1H), 0.78 (t, J=4.6 Hz, 1H). LCMS: [M+H]+=487.52.
A method similar to Example 1, General Method A heating a sealed, degassed mixture of 2-(4-acetylpiperazin-1-yl)pyridine-5-boronic acid pinacol ester (66 mg, 0.19 mmol), PdCl2dppf (12 mg, 0.016 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (67 mg, 0.13 mmol, 71% purity), Cs2CO3 (153 mg, 0.470 mmol), H2O (2.0 mL) and DME (4 mL) afforded the title compound as a clear film (48.8 mg 61%). 1H NMR (500 MHz, CD3OD) δ 8.25 (br d, J=9.3 Hz, 1H), 8.16 (s, 1H), 7.74 (br d, J=8.3 Hz, 2H), 7.43 (br d, J=9.4 Hz, 2H), 3.85 (br s, 6H), 3.76-3.82 (m, 2H), 3.55 (br t, J =5.9 Hz, 2H), 3.03 (br s, 2H), 2.18 (s, 3H). LCMS: [M+H]+=479.47.
A procedure similar to Example 218 using 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)spiro[chromane-2,4′-piperidin]-4-one (29.4 mg, 0.062 mmol) in CH2Cl2 (1.5 mL), acetone (1.5 mL) was added 2 drops of glacial AcOH (24 mg, 0.400 mmol) and NaH(OAc)3 (50 mg, 0.236 mmol) afforded the title compound (27.4 mg, 81%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.87 (s, 1H), 7.80 (br d, J=8.7 Hz, 1H), 7.64 (d, J=10.1 Hz, 1H), 7.47 (d, J=7.9 Hz, 1H), 7.45 (s, 1H), 7.15 (br d, J=7.8 Hz, 1H), 6.33 (s, 2H), 3.44-3.39 (m, 6H), 2.95 (br t, J=6.5 Hz, 2H), 2.87 (br s, 3H), 2.12-1.97 (m, 2H), 1.91 (s, 3H), 1.89-1.70 (m, 2H), 1.11 (br s, 6H); LCMS: [M+H]+=515.58.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(spiro[chromane-2,4′-piperidin]-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (29.4 mg, 0.064 mmol) in CH2Cl2 (1.5 mL), acetone (1.5 mL), glacial AcOH (24 mg, 0.400 mmol) and NaBH(OAc)3 (50 mg, 0.236 mmol) afforded the title compound (15.4 mg, 46%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.48-7.42 (m, 2H), 7.32-7.25 (m, 2H), 6.87 (br s, 1H), 6.21 (s, 2H), 3.44-3.38 (m, 5H), 2.95 (br t, J=6.5 Hz, 2H), 2.79 (br s, 2H), 2.06-1.93 (m, 2H), 1.91 (s, 3H), 1.82 (br s, 2H), 1.24 (br d, J=11.2 Hz, 6H); LCMS: [M+H]+=501.59.
A procedure similar to Example 218 using 5-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (26.1 mg, 0.057 mmol) in CH2Cl2 (1.5 mL), acetone (1.5 mL), 2 drops of glacial AcOH (24 mg, 0.400 mmol) and NaBH(OAc)3 (50 mg, 0.236 mmol) afforded the title compound (14.2 mg, 47%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 2H), 7.91 (d, J=7.9 Hz, 1H), 7.84 (br d, J=8.1 Hz, 1H), 7.71 (d, J=10.1 Hz, 1H), 7.48 (br d, J=8.2 Hz, 1H), 7.45 (s, 1H), 7.39 (br d, J=9.0 Hz, 1H), 6.39 (br s, 2H), 5.06-4.60 (m, 1H), 3.46-3.38 (m, 6H), 2.95 (br t, J=6.4 Hz, 3H), 2.32-2.06 (m, 4H), 1.91 (s, 3H), 1.30-1.07 (m, 6H); LCMS: [M+H]+=500.54.
To a CH2Cl2 (15 mL) solution of 6-(5-(4-((1R,5S)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (60 mg, 0.14 mmol) and i-Pr2NEt (0.063 mL, 0.36 mmol) was added 2-(dimethylamino)acetyl chloride·HCl (23 mg, 0.14 mmol) as solid in one portion at 0° C. The reaction was allowed to warm to rt and stirring was continued overnight. Later, i-Pr2NEt (0.15 mL, 0.87 mmol) was added followed by 2-(dimethylamino)acetylchloride·HCl (75 mg, 0.48 mmol) at 0° C. After stirring at 0° C. for 2 h excess 2 M NH3/MeOH was added. The reaction mixture was concentrated and purified by flash chromatography (25 g SiO2, using CH2Cl2/MeOH/aq NH4OH 89/10/1 in CH2Cl2) followed by a filtration through a Waters PoraPak CX column to afford the title compound (27.2 mg, 38%) as a cream white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.60-7.67 (m, 1H), 7.37-7.56 (m, 4H), 7.17-7.33 (m, 2H), 6.31 (br s, 2H), 4.02-4.21 (m, 1H), 3.88 (d, J=10.4 Hz, 0.5 H), 3.77 (d, J=11.5 Hz, 1H), 3.69 (dd, J=10.4, 3.9 Hz, 0.5H), 3.39-3.53 (m, 3H), 3.06-3.26 (m, 1H), 2.80-3.05 (m, 3H), 2.22 (d, J=2.1 Hz, 6H), 1.91-2.09 (m, 1H), 1.07-1.14 (m, 1H), 0.74 (t, J=4.4 Hz, 1H). LCMS: [M+H]+=500.54.
Step 1: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A, by heating a degassed, sealed mixture of H2O (6 mL), 5-bromo-6-fluoro-3-iodopyridin-2-amine (500 mg, 1.58 mmol), 7-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (505 mg, 1.74 mmol), Na2CO3 (334 mg, 3.16 mmol), Pd (PPh3)2Cl2 (111 mg, 0.158 mmol) and MeCN (12 mL) in a microwave reactor at 80° C. for 45 min and then again for 1 h, afforded, after workup and purification, the product as a brown solid (290 mg, 37% yield based on purity of 71%). LCMS: [M+H]+=354.34/356.30.
Step 2: 6-(6-amino-2-fluoro-6′-(piperazin-1-yl)-[3,3′-bipyridin]-5-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 1, General Method A using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.16 mmol), tert-butyl 4-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)piperazine-1-carboxylate (75 mg, 0.19 mmol, 71% purity), PdCl2dppf (12 mg, 0.016 mmol), Cs2CO3 (157 mg, 0.481 mmol), H2O (2.0 mL) and DME (4 mL) afforded the product as a light tan solid (64 mg, 65% yield based on purity of 71%). LCMS [M+H]+=437.49.
Step 3: 6-(6-amino-2-fluoro-6′-(4-isopropylpiperazin-1-yl)-[3,3′-bipyridin]-5-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A solution of 6-(6-amino-2-fluoro-6′-(piperazin-1-yl)-[3,3′-bipyridin]-5-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (65 mg, 0.106 mmol, 79% purity) in anh. CH2Cl2 (15 mL) was treated with acetone (0.039 mL, 0.53 mmol) at RT and glacial AcOH (0.024 mL, 0.42 mmol). The mixture was stirred at RT for 15 min before NaBH(OAc)3 (90 mg, 0.42 mmol) was added in one portion at RT. Stirring was continued for 2 d. Later, acetone (0.039 mL, 0.53 mmol) and after 3 h, NaBH(OAc)3 (90 mg, 0.42 mmol) were added in sequence. After 1 d at of stirring at RT, MeOH was added and the mixture was concentrated and purified by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in (H2O+0.05% TFA)) followed by a filtration through a Waters PoraPak CX column to afford the title compound as a very pale yellow solid (23.0 mg, 51% yield based on purity of 98%). 1H NMR (500 MHz, DMSO-d6) δ 8.28 (s, 1H), 8.11 (br s, 1H), 7.71 (br d, J=8.7 Hz, 1H), 7.62 (t, J=10.0 Hz, 2H), 7.43 (d, J=7.0 Hz, 1H), 6.88 (br d, J=8.8 Hz, 1H), 6.25 (s, 2H), 3.49 (br d, J=10.9 Hz, 4H), 3.39-3.45 (m, 3H), 2.93 (br t, J=6.4 Hz, 2H), 2.74 (br s, 1H), 2.54-2.66 (m, 3H), 0.96-1.12 (m, 6H). LCMS: [M+H]+=479.54.
A degassed solution mixture of 8-chloro-7-fluoro-6-iodoisoquinolin-3-amine (40 mg, 0.124 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (71.3 mg, 0.186 mmol), and bis(4-(di-tert-butylphosphanyl)-N,N′dimethylaniline)-palladium chloride (17.56 mg, 0.025 mmol) in 1,4-dioxane (10 mL) and 1 M aqueous K3PO4 solution (4.00 mL) was agitated at 90° C. over 60 min. The mixture was concentrated and extracted with EtOAc/water, the organic layer was concentrated and purified by silica gel chromatography (13 g column, eluting with 0-5-100% MeOH/water) to afford the title compound (11 mg, 19%) as a yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 8.95-9.03 (m, 1H), 7.93-7.97 (m, 1H), 7.89-7.93 (m, 1H), 7.70-7.74 (m, 1H), 7.65-7.70 (m, 1H), 7.43-7.50 (m, 2H), 6.67-6.72 (m, 1H), 6.51-6.62 (m, 2H), 6.18-6.27 (m, 2H), 3.36-3.45 (m, 2H), 2.91-3.00 (m, 2H); LCMS: [M+H]+=452.46.
A procedure analogous to Example 43, Step 5 using 2-bromo-6-isopropyl-5,6-dihydro-4H-pyrazolo[1,5-d][1,4]diazepin-7(8H)-one (35 mg, 0.129 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (73.9 mg, 0.193 mmol) and bis(di-tert-butyl(4-dimethylaminophenyl)phosphine)dichloropalladium(II) (91 mg, 0.129 mmol) in 1,4-dioxane (10 ml) and K3PO4 (2.00 mL of a 1.0 M aqueous solution) afforded the title compound (14 mg, 23%), as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.81-8.02 (m, 2H), 7.36-7.46 (m, 2H), 6.21-6.45 (m, 2H), 4.98-5.09 (m, 2H), 4.50-4.64 (m, 1H), 3.73-3.87 (m, 2H), 3.38-3.47 (m, 2H), 3.01-3.08 (m, 1H), 2.90-3.01 (m, 2H), 1.12 (s, 6H); LCMS: [M+H]+=449.45.
A procedure similar to Example 43, Step 5 using 2-bromo-5,6-dihydro-4H-pyrazolo[1,5-d][1,4]diazepin-7(8H)-one (35 mg, 0.152 mmol)), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (87 mg, 0.228 mmol), and bis(di-tert-butyl(4-dimethylaminophenyl)phosphine)dichloropalladium(II) (21.54 mg, 0.030 mmol) in 1,4-dioxane (10 mL) and K3PO4 (2.00 mL of a 1.0 M aqueous solution) afforded the title compound (4.4 mg, 7%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.09-8.25 (m, 1H), 7.82-8.01 (m, 3H), 7.37-7.49 (m, 2H), 6.25-6.44 (m, 3H), 5.72 (s, 2H), 4.92 (s, 2H), 3.94-4.26 (m, 4H), 2.99-3.06 (m, 2H), 2.93-3.00 (m, 2H); LCMS: [M+H]+=407.62
A procedure analogous to that of Example 43, step 5, by coupling 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.113 mmol) and 4-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)phenylboronic acid pinacol ester (44.1 mg, 0.147 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (12.45 mg, 0.017 mmol) and Cs2CO3 (111 mg, 0.340 mmol) in DME (3.5 mL) afforded the title compound as a beige solid (13 mg, 24%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.4-7.5 (m, 6H), 7.4-7.4 (m, 1H), 6.3-6.4 (m, 2H), 6.1-6.3 (m, 1H), 3.4-3.4 (m, 2H), 3.1-3.1 (m, 2H), 2.9-3.0 (m, 2H), 2.6-2.7 (m, 2H), 2.5-2.6 (m, 2H), 2.3-2.4 (m, 3H); LCMS: [M+H]+=445.54.
A procedure analogous to that of Example 43, step 5 using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.113 mmol) and 1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperidine (44.4 mg, 0.147 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (12.45 mg, 0.017 mmol) and Cs2CO3 (111 mg, 0.340 mmol) in DME (3.5 mL) afforded the title compound (26 mg, 49%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 3H), 7.3-7.3 (m, 2H), 6.3-6.4 (m, 2H), 3.4-3.4 (m, 2H), 2.9-3.0 (m, 2H), 2.8-2.9 (m, 2H), 2.4-2.5 (m, 1H), 2.2-2.2 (m, 3H), 1.9-2.0 (m, 2H), 1.7-1.8 (m, 4H); LCMS: [M+H]+=447.67.
A procedure analogous to that of Example 43, Step 5, 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.108 mmol), 4-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)phenylboronic acid pinacol ester (42.0 mg, 0.140 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (11.85 mg, 0.016 mmol) and Cs2CO3 (105 mg, 0.324 mmol) in DME (3.5 mL) afforded the title compound as a yellow powder (17 mg, 31%). 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.2 (m, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 4H), 6.35 (s, 2H), 6.2-6.3 (m, 1H), 3.4-3.5 (m, 4H), 3.0-3.1 (m, 2H), 2.9-2.9 (m, 2H), 2.6-2.6 (m, 2H), 2.3-2.3 (m, 3H); LCMS: [M+H]+=463.53.
A procedure analogous to that of Example 43, step 5 using 6-(2-amino-5-bromo-6-chloropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.108 mmol), 1-methyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperidine (42.3 mg, 0.140 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (11.85 mg, 0.016 mmol) and Cs2CO3 (105 mg, 0.324 mmol) in DME (3.5 mL) and H2O (1 mL) afforded the title compound (17 mg, 31%) as a yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.2 (m, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 1H), 7.3-7.4 (m, 3H), 7.2-7.3 (m, 2H), 6.2-6.4 (m, 2H), 3.4-3.4 (m, 2H), 2.8-2.9 (m, 4H), 2.4-2.5 (m, 1H), 2.2-2.2 (m, 3H), 1.9-2.1 (m, 2H), 1.7-1.8 (m, 2H), 1.6-1.7 (m, 2H); LCMS: [M+H]+=465.60.
Step 1: bis-tert-butyl (6′-amino-2′-fluoro-4-methyl-5′-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)-[3,3′-bipyridin]-5-yl)carbamate
A degassed solution mixture of bis-tert-butyl (5-bromo-4-methylpyridin-3-yl)carbamate (40 mg, 0.103 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (59.4 mg, 0.155 mmol) and bis(di-tert-butyl(4-dimethylaminophenyl)phosphine)dichloropalladium(II) (14.63 mg, 0.021 mmol) in 1,4-Dioxane (10 mL) and aqueous K3PO4 (2.0 mL of a 1.0 M solution) was agitated at 90° C. for 60 min. The mixture was concentrated and partitioned between EtOAc and water. The separated organic layer was concentrated and purified by column chromatography (13 g reverse phase column, 0-5-100% ACN/water) to afford the product (26 mg, 45% yield), as a white powder. LCMS: [M+H]+=564.57.
Step 2: 6-(5′,6-diamino-2-fluoro-4′-methyl-[3,3′-bipyridin]-5-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetic acid salt
A mixture of bis-tert-butyl (6′-amino-2′-fluoro-4-methyl-5′-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)-[3,3′-bipyridin]-5-yl)carbamate (26 mg, 0.046 mmol) in 1:1 TFA/DCM (2 mL) was stirred at 25° C. for 2 h and then concentrated and purified by silica gel chromatography (eluting with DCM/MeOH 0-5-30%) to afford, after lyophilization, the TFA salt of the title compound (12 mg, 23%) as a white powder. 1H NMR (500 MHz, CD3OD) δ 7.90-7.97 (m, 1H), 7.83-7.90 (m, 1H), 7.71-7.81 (m, 1H), 7.38-7.43 (m, 2H), 7.34-7.37 (m, 1H), 3.40-3.53 (m, 2H), 2.87-3.03 (m, 2H), 2.04-2.15 (m, 3H); LCMS: [M+H]+=364.55.
A degassed mixture of 6-(2-amino-5-(4-(2,5-dihydro-1 H-pyrrol-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (60 mg, 0.15 mmol) and Pd/C (10 wt %, 16 mg, 0.015 mmol) in EtOH (10 mL) was stirred under H2 (1 atm) at RT for 3 h. The mixture was filtered through Celite® using MeOH—CH2Cl2. Concentration of the filtrate afforded 6-(2-amino-6-fluoro-5-(4-(pyrrolidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one. This material was taken up in anh. DMF (2 mL) and treated with Et3N (0.21 mL, 1.5 mmol) and ethyl bromide (0.022 mL, 0.30 mmol) at RT. The mixture was stirred at RT for 18 h, concentrated and purified by flash chromatography (25 g SiO2, using MeOH in CH2Cl2 0 to 14% then 2 M NH3/MeOH in CH2Cl2) followed by a filtration through a Waters PoraPak CX column to afford the title compound as a white solid (19.0 mg, 30% yield for 2 steps). 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=8.0 Hz, 1H), 7.63 (d, J=10.2 Hz, 1H), 7.44-7.50 (m, 4H), 7.33 (d, J=8.2 Hz, 2H), 6.29 (s, 2H), 3.39-3.50 (m, 4H), 2.91-3.01 (m, 3H), 2.60-2.77 (m, 2H), 2.43-2.49 (m, 2H), 2.21-2.30 (m, 1H), 1.73-1.82 (m, 1H), 1.07 (t, J=7.2 Hz, 3H); LCMS: [M+H]+=431.54.
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.045 mmol) and N,N-diisopropylethylamine (0.079 ml, 0.455 mmol) in DMF (1.0 mL) was added 2,2-difluoroethyl trifluoromethanesulfonate (14.61 mg, 0.068 mmol). The reaction was stirred at RT for 1 h, concentrated in vacuo and purified by Waters prep HPLC (XBridge Prep C18 5 μm, 10×100 mm Column, 80-70% 0.1% formic acid in water/0.1% formic acid in acetonitrile) to afford the title compound (13.6 mg, 60% yield) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.60 (dd, J=12.5, 10.2 Hz, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.39 (d, J=7.8 Hz, 2H), 6.98 (d, J=8.9 Hz, 2H), 6.16-6.22 (m, 2H), 3.41 (td, J=6.5, 2.7 Hz, 2H), 3.15-3.21 (m, 4H), 2.93 (br t, J=6.4 Hz, 2H), 2.80 (td, J=15.7, 4.3 Hz, 2H), 2.66-2.71 (m, 4H); LCMS: [M+H]+=500.57.
A procedure similar to Example 236 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one TFA (25 mg, 0.045 mmol), N,N-diisopropylethylamine (0.079 ml, 0.455 mmol), DMF (1.0 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (15.84 mg, 0.068 mmol) afforded the title compound (18.2 mg, 77%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (br s, 1H), 7.60 (t, J=10.9 Hz, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.39 (br d, J=8.0 Hz, 2H), 6.98 (d, J=8.9 Hz, 2H), 6.19 (s, 2H), 3.40 (br dd, J=6.5, 2.4 Hz, 2H), 3.25 (q, J=10.3 Hz, 2H), 3.15-3.21 (m, 4H), 2.93 (br t, J=6.4 Hz, 2H), 2.73-2.81 (m, 4H); LCMS: [M+H]+=518.62.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate
To a suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (370 mg, 1.05 mmol), 4-(N-Boc-piperidino)phenylboronic acid pinacol ester (486 mg, 1.25 mmol) and Cs2CO3 (851 mg, 2.61 mmol) in 3:1 DME/H2O (16 mL) was added PdCl2dppf (76 mg, 0.104 mmol). The mixture was flushed with nitrogen and heated in a microwave apparatus at 90° C. for 2.5 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-100% EtOAc/Hexanes to give the product (498 mg, 0.932 mmol, 89%) as a brown solid. LCMS: [M+H]+=535.67.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate (498 mg, 0.932 mmol) in CH2Cl2 (5.0 ml) was added TFA (2.1 mL, 27.9 mmol). The reaction was stirred at RT for 3 d. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give TFA salt of the title compound (464 mg, 91%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.18-8.44 (m, 1H), 8.12 (br s, 1H), 7.63 (t, J=10.4 Hz, 2H), 7.51 (br d, J=7.7 Hz, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.28 (d, J=8.2 Hz, 2H), 6.32 (s, 2H), 3.37-3.44 (m, 4H), 2.96-3.04 (m, 2H), 2.93 (br t, J=6.4 Hz, 2H), 2.82-2.90 (m, 1H), 1.96 (br d, J=13.2 Hz, 2H), 1.72-1.86 (m, 2H); LCMS: [M+H]+=435.64.
A mixture of 6-(2-amino-6-fluoro-5-(4-(4-fluoropiperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (55 mg, 0.114 mmol), 2-iodopropane (77 mg, 0.456 mmol) DMF (2.0 mL) and Et3N (0.159 ml, 1.139 mmol) was stirred overnight at RT. The reaction mixture was concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-3% MeOH and 0-0.3% NH4OH), followed by filtration through a Waters PoraPak CX column afforded the title compound (18 mg, 32%) as an off white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 2H), 7.4-7.5 (m, 4H), 6.2-6.4 (m, 2H), 3.4-3.5 (m, 3H), 2.9-3.0 (m, 2H), 2.7-2.9 (m, 3H), 2.5-2.6 (m, 1H), 2.0-2.2 (m, 2H), 1.9-2.0 (m, 2H), 1.0-1.1 (m, 6H); LCMS: [M+H]+=477.63.
A procedure similar to Example 236 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (30 mg, 0.056 mmol) and N,N-diisopropylethylamine (0.098 ml, 0.564 mmol) in DMF (1.0 ml) and 1,1,1-trifluoro-3-iodopropane (18.96 mg, 0.085 mmol) afforded the title compound (10.6 mg, 37%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br s, 1H), 7.83 (d, J=8.0 Hz, 1H), 7.51 (d, J=10.2 Hz, 1H), 7.39 (br d, J=8.0 Hz, 1H), 7.37 (s, 1H), 7.33 (br d, J=8.0 Hz, 2H), 6.91 (d, J=8.9 Hz, 2H), 6.10 (s, 2H), 3.33 (td, J=6.4, 2.5 Hz, 2H), 3.07-3.12 (m, 4H), 2.88 (br t, J=6.4 Hz, 2H), 2.42-2.44 (m, 8H); LCMS: [M+H]+=514.48.
A procedure analogous to Example 236 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (30 mg, 0.055 mmol), N,N-diisopropylethylamine (0.095 ml, 0.547 mmol), DMF (1.0 mL) and 2-bromoethyl methyl ether (38.0 mg, 0.273 mmol) afforded the title compound (25.1 mg, 93%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.12 (br s, 1H), 7.59-7.68 (m, 2H), 7.50 (br d, J=7.2 Hz, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.30 (br d, J=7.6 Hz, 2H), 6.31 (br s, 2H), 3.63-3.74 (m, 2H), 3.40-3.44 (m, 3H), 2.93 (br t, J=6.4 Hz, 2H), 1.94 (br d, J=19.2 Hz, 4H), 1.22-1.31 (m, 3H); LCMS: [M+H]+=493.61.
A procedure analogous to Example 236 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1 (2H)-one, trifluoroacetate (30 mg, 0.055 mmol) and N,N-diisopropylethylamine (0.095 mL, 0.546 mmol), DMF (1.0 ml) and 1,1,1-trifluoro-3-iodopropane (36.7 mg, 0.164 mmol) afforded the title compound (14.6 mg, 50%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (br s, 1H), 7.60 (dd, J=13.1, 10.3 Hz, 2H), 7.43 (br d, J=7.0 Hz, 1H), 7.39 (br d, J=8.1 Hz, 2H), 6.98 (br d, J=8.8 Hz, 2H), 6.18 (s, 2H), 3.41 (br dd, J=6.1, 4.0 Hz, 2H), 3.16 (br d, J=5.0 Hz, 4H), 2.93 (br t, J=6.3 Hz, 2H), 2.57 (br d, J=4.8 Hz, 4H), 1.27 (br s, 4H); LCMS: [M+H]+=532.66.
A procedure analogous to Example 236 was used, stirring 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (30 mg, 0.055 mmol), N,N-diisopropylethylamine (0.095 ml, 0.546 mmol), DMF (1.0 mL) and 1,1,1-trifluoro-3-iodopropane (36.7 mg, 0.164 mmol) for 16 h. Additional 1,1,1-trifluoro-3-iodopropane (37 mg) was added and the mixture was stirred at RT for another 16 h. Workup and purification afforded the title compound (14.6 mg, 50%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ pm 8.10 (br s, 1H), 7.60 (dd, J=13.1, 10.3 Hz, 2H), 7.43 (br d, J=7.0 Hz, 1H), 7.39 (br d, J=8.1 Hz, 2H), 6.98 (br d, J=8.8 Hz, 2H), 6.18 (s, 2H), 3.41 (br dd, J=6.1, 4.0 Hz, 2H), 3.16 (br d, J=5.0 Hz, 4H), 2.93 (br t, J=6.3 Hz, 2H), 2.57 (br d, J=4.8 Hz, 4H), 1.27 (br s, 4H); LCMS: [M+H]+=532.66.
A procedure similar to Example 236 was used with 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol) and N,N-diisopropylethylamine (0.079 ml, 0.456 mmol) in DMF (1.0 mL). 2-Iodopropane (38.7 mg, 0.228 mmol) was added and the mixture was stirred at RT for 16 h, then more 2-iodopropane (39 mg) was added and at the mixture was stirred at RT for 16 h. Workup and purification afforded the title compound (17.1 mg, 79%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.63 (t, J=9.9 Hz, 2H), 7.49 (br d, J=7.6 Hz, 2H), 7.43 (d, J=7.0 Hz, 1H), 7.29 (d, J=8.2 Hz, 2H), 6.30 (s, 2H), 2.93 (br t, J=6.4 Hz, 2H), 2.65-2.80 (m, 2H), 1.92 (br s, 2H), 1.70-1.86 (m, 2H), 1.11-1.27 (m, 7H); LCMS: [M+H]+=477.69.
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol) and N,N-diisopropylethylamine (0.079 mL, 0.456 mmol) in DMF (1.0 mL) was added bromoethane (24.83 mg, 0.228 mmol). The reaction was stirred at RT over 2 d. Workup and purification in a manner similar to Example 262 afforded the title compound (17.6 mg, 0.038 mmol, 83%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.63 (t, J=10.3 Hz, 2H), 7.50 (br d, J=7.6 Hz, 2H), 7.43 (d, J=7.0 Hz, 1H), 7.30 (br d, J=8.2 Hz, 2H), 6.30 (s, 2H), 3.10 (br s, 1H), 2.89-2.99 (m, 4H), 2.78 (br s, 2H), 1.97 (br d, J=12.4 Hz, 2H), 1.79-1.92 (m, 2H), 1.25 (br d, J=6.5 Hz, 4H), 1.18-1.23 (m, 3H); LCMS: [M+H]+=463.65.
To a suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.085 mmol), 4-(4-methylpiperazin-1-yl)phenylboronic acid, pinacol ester (30.7 mg, 0.102 mmol) and Cs2CO3 (69.0 mg, 0.212 mmol) in a 3:1 mixture of DME (4 mL): water (1.3 mL) was added PdCl2dppf (6.20 mg, 8.47 μmol). The mixture was flushed with nitrogen and heated in the microwave at 90° C. for 2.5 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography (eluting with 0-10% MeOH/DCM+1% NH4OH), followed by reverse phase chromatography (C18, 0-100% ACN/H2O) to afford the title compound (28.9 mg, 76%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (br s, 1H), 7.60 (br dd, J=13.5, 10.3 Hz, 2H), 7.43 (br d, J=6.9 Hz, 1H), 7.38 (br d, J=8.3 Hz, 2H), 6.97 (br d, J=8.4 Hz, 2H), 6.18 (br s, 2H), 3.41 (br d, J=4.2 Hz, 2H), 3.16 (br s, 4H), 2.93 (br t, J=6.1 Hz, 2H), 2.46 (br s, 4H), 2.23 (s, 3H); LCMS: [M+H]+=450.68.
A procedure analogous to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.085 mmol), 3-fluoro-4-(4-methylpiperazino)phenylboronic acid pinacol ester (32.6 mg, 0.102 mmol) Cs2CO3 (69.0 mg, 0.212 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (6.20 mg, 8.47 μmol) afforded the title compound (28.4 mg, 72%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.66 (d, J=10.2 Hz, 1H), 7.61 (d, J=10.0 Hz, 1H), 7.43 (d, J=7.1 Hz, 1H), 7.33 (br d, J=14.6 Hz, 1H), 7.29 (br d, J=8.6 Hz, 1H), 7.05 (t, J=9.0 Hz, 1H), 6.31 (s, 2H), 3.41 (td, J=6.4, 2.6 Hz, 2H), 3.04 (br s, 4H), 2.93 (br t, J=6.4 Hz, 2H), 2.48 (br s, 4H), 2.23 (s, 3H); LCMS: [M+H]+=468.54.
A procedure analogous to Example 236 stirring 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol), N,N-diisopropylethylamine (0.079 mL, 0.456 mmol), DMF (1.0 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (21.16 mg, 0.091 mmol) at RT over 2 d afforded the title compound (13.7 mg, 58%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.03 (br s, 1H), 7.55 (t, J=10.8 Hz, 2H), 7.33-7.42 (m, 3H), 7.23 (br d, J=8.2 Hz, 2H), 6.21 (s, 2H), 3.33 (br s, 3H), 3.12 (br d, J=10.3 Hz, 2H), 2.95 (br d, J=11.3 Hz, 2H), 2.85 (br t, J=6.3 Hz, 2H), 2.34-2.40 (m, 2H), 1.64-1.71 (m, 2H), 1.55-1.64 (m, 2H); LCMS: [M+H]+=517.37.
A procedure analogous to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.085 mmol), 3-(trifluoromethyl)-4-(N-methylpiperazin-1-yl)phenyl boronic acid pinacol ester (37.6 mg, 0.102 mmol), Cs2CO3 (69.0 mg, 0.212 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (6.20 mg, 8.47 μmol) afforded the title compound (14.2 mg, 32%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.04 (br s, 1H), 7.73 (br d, J=8.6 Hz, 1H), 7.70 (s, 1H), 7.67 (d, J=10.2 Hz, 1H), 7.48-7.57 (m, 2H), 7.36 (d, J=7.0 Hz, 1H), 6.34 (s, 2H), 3.34 (td, J=6.5, 2.7 Hz, 2H), 2.80-2.90 (m, 6H), 2.19 (br s, 3H); LCMS: [M+H]+=518.62.
A procedure analogous to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), (2S,6R)-4-(5-fluoro-2-nitro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1,2,6-trimethylpiperazine (70.2 mg, 0.178 mmol, prepared according to methods described in WO 2017/147700), Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (15.7 mg, 19%) as a bright yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 8.30 (br s, 1H), 8.02 (d, J=8.0 Hz, 1H), 7.95 (br s, 1H), 7.91 (d, J=8.0 Hz, 1H), 7.60 (d, J=9.7 Hz, 1H), 7.46 (d, J=8.1 Hz, 1H), 7.44 (s, 1H), 7.20 (d, J=12.5 Hz, 1H), 6.46 (s, 2H), 3.10 (br d, J=12.1 Hz, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.69 (br t, J=11.4 Hz, 2H), 2.24-2.31 (m, 2H), 2.20 (s, 3H), 1.02 (d, J=6.1 Hz, 6H); LCMS: [M+H]+=523.63.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-4-fluoropiperidine-1-carboxylate
A procedure analogous to that of Example 43, Step 5 with tert-butyl 4-(4-chlorophenyl)-4-fluoropiperidine-1-carboxylate (72 mg, 0.218 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (117 mg, 0.305 mmol) afforded the product as a white solid (123 mg, 100% yield based on purity of 95%). LCMS: [M+H]+=535.60.
Step 2: 6-(2-Amino-6-fluoro-5-(4-(4-fluoropiperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A solution 4.0 M HCl in dioxane (1.37 mL, 5.46 mmol) was added to a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-4-fluoropiperidine-1-carboxylate (123 mg, 0.219 mmol) in MeOH (4 mL), and the reaction mixture was stirred overnight at RT. The reaction mixture was concentrated to dryness, the residue was dissolved in MeOH and passed through a Waters PoraPak CX column to afford the product as a white powder (106 mg, 100% yield based on purity of 90%). LCMS: [M+H]+=415.52 (-F).
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-fluoro-1-(2-methoxyethyl)piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 90, Step 2, by stirring 6-(2-amino-6-fluoro-5-(4-(4-fluoropiperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.104 mmol), and 2-bromoethyl methyl ether (0.039 mL, 0.414 mmol) overnight at RT, afforded the title compound as a yellow powder (6.5 mg, 12%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 2H), 7.4-7.5 (m, 4H), 6.3-6.5 (m, 2H), 3.5-3.5 (m, 2H), 3.4-3.4 (m, 2H), 3.2-3.3 (m, 3H), 2.9-3.0 (m, 2H), 2.7-2.9 (m, 2H), 2.5-2.6 (m, 2H), 2.3-2.4 (m, 2H), 2.0-2.2 (m, 2H), 1.8-2.0 (m, 2H); LCMS: [M+H]+=493.61.
A procedure analogous to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.085 mmol), 2-chloro-4-(n-methylpiperazin-1-yl)phenylboronic acid (25.9 mg, 0.102 mmol) and Cs2CO3 (69.0 mg, 0.212 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (6.20 mg, 8.47 μmol) afforded the title compound (14.6 mg, 36%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (br s, 1H), 7.61 (d, J=10.2 Hz, 1H), 7.36-7.43 (m, 2H), 7.23 (d, J=8.6 Hz, 1H), 7.05 (d, J=2.1 Hz, 1H), 6.96 (dd, J=8.6, 2.1 Hz, 1H), 6.28 (s, 2H), 3.30-3.37 (m, 4H), 3.23 (br s, 4H), 2.92 (br t, J=6.3 Hz, 2H), 2.27 (br s, 3H); LCMS: [M+H]+=484.65.
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 1-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)indoline (46.3 mg, 0.178 mmol) and Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (26.9 mg, 47%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.91 (br d, J=7.8 Hz, 1H), 7.54 (br d, J=10.2 Hz, 1H), 7.47 (br d, J=8.2 Hz, 1H), 7.44 (s, 1H), 7.23 (br s, 1H), 7.20 (br d, J=8.0 Hz, 1H), 6.54 (br d, J=8.1 Hz, 1H), 6.11 (br s, 2H), 3.40-3.43 (m, 2H), 3.28 (br t, J=8.1 Hz, 2H), 2.96 (br t, J=6.1 Hz, 2H), 2.90 (br t, J=8.1 Hz, 2H), 2.72 (s, 3H); LCMS: [M+H]+=389.63.
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 2-methyl-1,2,3,4-tetrahydroisoquinoline-6-boronic acid pinacol ester (48.8 mg, 0.178 mmol), Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL), PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (31.7 mg, 53%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br s, 1H), 7.84 (d, J=8.0 Hz, 1H), 7.55 (d, J=10.0 Hz, 1H), 7.40 (br d, J=8.1 Hz, 1H), 7.23 (br s, 1H), 7.21 (s, 1H), 7.01 (d, J=8.2 Hz, 1H), 6.19 (s, 2H), 3.42 (s, 2H), 3.31-3.37 (m, 3H), 2.88 (br t, J=6.5 Hz, 2H), 2.77 (br t, J=5.6 Hz, 2H), 2.52 (br t, J=5.8 Hz, 2H), 2.27 (s, 3H); LCMS: [M+H]+=403.61.
Step 1: tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinoline-2(1H)-carboxylate
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (230 mg, 0.684 mmol), tert-butyl 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinoline-2(1 H)-carboxylate (295 mg, 0.821 mmol), Cs2CO3 (557 mg, 1.71 mmol) DME (4 mL), water (1.3 mL) and PdCl2dppf (50.1 mg, 0.068 mmol) afforded the product (294 mg, 88%) as a beige solid. LCMS: [M+H]+=489.60.
Step 2: 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinoline-2(1 H)-carboxylate (294 mg, 0.602 mmol) in CH2Cl2 (5.0 mL) was added TFA (1.38 mL, 18.1 mmol). The reaction was stirred at RT overnight. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to afford 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate salt (266 mg, 88%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.74-8.92 (m, 1H), 7.96 (br s, 1H), 7.92 (d, J=8.0 Hz, 1H), 7.65 (d, J=10.2 Hz, 1H), 7.42-7.48 (m, 4H), 7.26 (d, J=8.0 Hz, 1H), 6.35 (s, 2H), 4.28 (s, 2H), 3.38-3.44 (m, 4H), 3.02 (br t, J=6.1 Hz, 2H), 2.96 (t, J=6.5 Hz, 2H); LCMS: [M+H]+=389.63.
A procedure similar to Example 236 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol) and N,N-diisopropylethylamine (0.079 mL, 0.456 mmol), DMF (1.0 mL) and 2,2-difluoroethyl trifluoromethanesulfonate (19.52 mg, 0.091 mmol) afforded the title compound (9.3 mg, 41%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.03 (br s, 1H), 7.55 (t, J=10.3 Hz, 2H), 7.38 (br d, J=7.2 Hz, 2H), 7.36 (d, J=7.1 Hz, 1H), 7.22 (d, J=8.2 Hz, 2H), 6.20-6.24 (m, 2H), 3.33 (td, J=6.5, 2.7 Hz, 2H), 2.94 (br d, J=11.4 Hz, 2H), 2.85 (br t, J=6.4 Hz, 2H), 2.68 (td, J=15.7, 4.3 Hz, 2H), 2.16-2.24 (m, 2H), 1.65-1.71 (m, 2H), 1.54-1.64 (m, 2H); LCMS: [M+H]+=499.63.
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl]morpholine (54.1 mg, 0.178 mmol), Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL), and PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (29.7 mg, 46%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (br d, J=8.0 Hz, 1H), 7.65 (br d, J=10.0 Hz, 1H), 7.52 (br d, J=7.6 Hz, 2H), 7.48 (br d, J=8.2 Hz, 1H), 7.46 (s, 1H), 7.35 (br d, J=8.0 Hz, 2H), 6.31 (br s, 2H), 3.58 (br s, 4H), 3.48 (s, 2H), 3.41 (br d, J=1.6 Hz, 2H), 2.96 (br t, J=6.2 Hz, 2H), 2.37 (br s, 4H); LCMS: [M+H]+=433.57.
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 4-((4-methylpiperazin-1-yl)methyl)phenylboronic acid pinacol ester (56.4 mg, 0.178 mmol), Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (20.2 mg, 31%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br s, 1H), 7.84 (d, J=7.8 Hz, 1H), 7.57 (d, J=10.2 Hz, 1H), 7.43 (br d, J=7.5 Hz, 2H), 7.39-7.42 (m, 1H), 7.38 (s, 1H), 7.26 (d, J=8.2 Hz, 2H), 6.23 (s, 2H), 3.39 (s, 2H), 3.34 (td, J=6.5, 2.5 Hz, 2H), 2.88 (br t, J=6.5 Hz, 2H), 2.17-2.38 (m, 6H), 2.07 (s, 3H); LCMS: [M+H]+=446.60.
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 4-(4-methylpiperazine-1-carbonyl)phenylboronic acid, pinacol ester (58.9 mg, 0.178 mmol) and Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (10.88 mg, 0.015 mmol) afforded (40.6 mg, 59%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.88 (br s, 1H), 7.84 (d, J=8.0 Hz, 1H), 7.63 (d, J=10.2 Hz, 1H), 7.56 (d, J=7.5 Hz, 2H), 7.40-7.44 (m, 1H), 7.39 (s, 1H), 7.36 (d, J=8.3 Hz, 2H), 6.33 (s, 2H), 3.54 (br s, 2H), 3.34 (td, J=6.5, 2.7 Hz, 2H), 2.88 (t, J=6.5 Hz, 2H), 2.26 (br s, 4H), 2.13 (s, 3H); LCMS: [M+H]+=460.64.
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 1-methyl-4-(1-[4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-phenyl]cyclopropyl)piperazine (61.1 mg, 0.178 mmol) Cs2CO3 (121 mg, 0.372 mmol), DME (3 mL), water (1 mL) and PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (52.1 mg, 74%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br s, 1H), 7.83 (d, J=8.0 Hz, 1H), 7.58 (d, J=10.2 Hz, 1H), 7.44 (br d, J=7.5 Hz, 2H), 7.40-7.42 (m, 1H), 7.39 (s, 1H), 7.22 (d, J=8.2 Hz, 2H), 6.25 (s, 2H), 3.34 (td, J=6.5, 2.6 Hz, 2H), 2.88 (br t, J=6.5 Hz, 2H), 2.37 (br s, 4H), 2.16 (br s, 4H), 1.99 (s, 3H), 0.78 (br d, J=1.7 Hz, 2H), 0.68 (br d, J=1.8 Hz, 2H); LCMS: [M+H]+=472.68.
A procedure similar to Example 281 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (21.4 mg, 0.043 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL), 2 drops of glacial AcOH (15.41 mg, 0.257 mmol) and NaBH(OAc)3 (54.4 mg, 0.257 mmol) afforded the title compound (23.1 mg, 95%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.76 (br d, J=8.9 Hz, 1H), 7.73 (s, 1H), 7.68 (d, J=10.1 Hz, 1H), 7.47 (d, J=7.9 Hz, 1H), 7.45 (s, 1H), 7.34 (d, J=8.9 Hz, 1H), 6.33 (s, 2H), 4.70-4.63 (m, 1H), 3.43-3.40 (m, 4H), 2.99-2.92 (m, 2H), 2.74-2.67 (m, 1H), 2.67-2.61 (m, 2H), 2.41-2.34 (m, 2H), 1.95-1.90 (m, 2H), 1.73-1.63 (m, 2H), 0.96 (d, J=6.6 Hz, 6H); LCMS: [M+H]+=543.65.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24 mg, 0.048 mmol) and N,N-diisopropylethylamine (0.084 ml, 0.480 mmol) in DMF (1 mL) with 2-bromoethyl methyl ether (0.014 mL, 0.144 mmol) afforded the title compound (21.1 mg, 75%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.77 (br d, J=8.8 Hz, 1H), 7.74 (s, 1H), 7.68 (d, J=10.3 Hz, 1H), 7.47 (d, J=7.9 Hz, 1H), 7.45 (s, 1H), 7.36 (br d, J=8.8 Hz, 1H), 6.34 (s, 2H), 4.81-4.64 (m, 1H), 3.54-3.44 (m, 2H), 3.40 (dt, J=2.6, 6.5 Hz, 3H), 3.25 (s, 3H), 2.95 (br t, J=6.5 Hz, 2H), 2.87-2.59 (m, 3H), 2.46-2.22 (m, 1H), 2.07-1.92 (m, 2H), 1.86-1.65 (m, 2H), 1.33-1.15 (m, 1H); LCMS: [M +H]+=559.67.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (21.3 mg, 0.042 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL) and 2 drops glacial AcOH (15.24 mg, 0.254 mmol) and NaBH(OAc)3 (53.8 mg, 0.254 mmol) afforded the title compound (24.1 mg, 99%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.12 (br s, 1H), 7.84 (br d, J=7.1 Hz, 1H), 7.80 (br s, 1H), 7.75 (d, J=10.1 Hz, 1H), 7.61 (d, J=10.1 Hz, 1H), 7.57 (d, J=8.4 Hz, 1H), 7.43 (d, J=7.0 Hz, 1H), 6.43 (br s, 2H), 3.44-3.38 (m, 4H), 3.17-2.95 (m, 4H), 2.95-2.89 (m, 3H), 1.91 (s, 2H), 1.37-1.03 (m, 6H), 1.53-1.42 (m, 2H); LCMS: [M+H]+=546.58.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (20.6 mg, 0.041 mmol) and N,N-diisopropylethylamine (0.071 ml, 0.409 mmol) in DMF (1 mL) with 2-bromoethyl methyl ether (0.012 ml, 0.123 mmol) afforded the title compound (18.4 mg, 76%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.79 (br d, J=8.6 Hz, 1H), 7.76 (s, 1H), 7.73 (d, J=10.3 Hz, 1H), 7.59 (dd, J=13.3, 9.2 Hz, 2H), 7.43 (d, J=7.0 Hz, 1H), 6.40 (s, 2H), 3.46 (s, 2H), 3.43-3.38 (m, 3H), 3.25 (s, 3H), 2.96-2.84 (m, 6H), 2.62-2.52 (m, 4H); LCMS: [M+H]+=562.53.
Step 1: tert-butyl 4-(3-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)benzyl)piperazine-1-carboxylate
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (250 mg, 0.744 mmol), tert-butyl 4-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)piperazine-1-carboxylate (359 mg, 0.892 mmol) and Cs2CO3 (606 mg, 1.86 mmol), DME (4 mL), water (1.3 mL), PdCl2dppf (54.4 mg, 0.074 mmol) afforded the title compound (336 mg, 85%) as a beige solid. LCMS: [M+H]+=532.72.
Step 2: 6-(2-amino-6-fluoro-5-(3-(piperazin-1-ylmethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A solution of tert-butyl 4-(3-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)benzyl)piperazine-1-carboxylate (336 mg, 0.632 mmol) in CH2Cl2 (3.0 mL) was added TFA (1.45 mL, 19.0 mmol). The reaction was stirred at RT overnight. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the trifluoroacetate salt (287 mg, 0.526 mmol, 83% yield) as a white solid. A portion (25 mg) of the material was further purified by Waters prep HPLC (XBridge Prep C18 5 μm, 10×100 mm column, 95-80% 0.1% formic acid in water/0.1% formic acid in acetonitrile) to afford the title compound (20.2 mg). 1H NMR (500 MHz, DMSO-d6) δ 8.30 (br s, 1H), 7.96 (br s, 1H), 7.92 (d, J=7.8 Hz, 1H), 7.64 (d, J=10.2 Hz, 1H), 7.43-7.51 (m, 4H), 7.38 (t, J=7.6 Hz, 1H), 7.26 (d, J=7.6 Hz, 1H), 6.33 (s, 2H), 3.53 (s, 2H), 3.41-3.43 (m, 2H), 2.96 (br t, J=6.5 Hz, 2H), 2.88 (br s, 4H), 2.44 (br s, 4H); LCMS: [M+H]+=432.63.
A procedure similar to Example 236 stirring 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.050 mmol), N,N-diisopropylethylamine (0.087 ml, 0.498 mmol), DMF (1.0 mL) and 2-bromoethyl methyl ether (34.6 mg, 0.249 mmol) The reaction was stirred at RT over 72 h afforded the title compound (7.2 mg, 32%) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) b 7.87 (br s, 1H), 7.84 (d, J=8.0 Hz, 1H), 7.54 (d, J=10.2 Hz, 1H), 7.40 (br d, J=8.0 Hz, 1H), 7.37 (s, 1H), 7.19-7.24 (m, 2H), 7.01 (d, J=8.0 Hz, 1H), 6.20 (s, 2H), 3.54 (s, 2H), 3.46 (t, J=5.8 Hz, 2H), 3.32-3.34 (m, 2H), 3.20 (s, 3H), 2.88 (br t, J=6.4 Hz, 2H), 2.76 (br t, J=5.5 Hz, 2H), 2.62-2.67 (m, 2H), 2.58 (t, J=5.9 Hz, 2H); LCMS: [M+H]+=447.61.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (23 mg, 0.053 mmol) in CH2Cl2 (1.5 mL), acetone (1.5 mL), 2 drops of glacial AcOH (19.16 mg, 0.319 mmol) and NaBH(OAc)3 (67.6 mg, 0.319 mmol) afforded the title compound (20.2 mg, 76%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.60 (d, J=10.3 Hz, 1H), 7.51-7.42 (m, 4H), 7.03 (br d, J=8.2 Hz, 2H), 6.23 (s, 2H), 4.72-4.42 (m, 1H), 3.45-3.38 (m, 6H), 3.14-3.00 (m, 2H), 2.95 (br t, J=6.5 Hz, 3H), 2.11 (br d, J=3.9 Hz, 2H), 1.87-1.70 (m, 1H), 1.33-1.04 (m, 6H); LCMS: [M+H]+=475.63.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(3-fluoro-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (22.7 mg, 0.050 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL), 2 drops of AcOH (18.16 mg, 0.302 mmol) and NaBH(OAc)3 (64.1 mg, 0.302 mmol) afforded the title compound (17.1 mg, 66%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.66 (d, J=10.1 Hz, 1H), 7.51-7.43 (m, 3H), 7.35-7.31 (m, 1H), 7.28 (br d, J=8.2 Hz, 1H), 6.33 (s, 2H), 4.70-4.44 (m, 1H), 3.40 (dt, J=6.5, 2.7 Hz, 3H), 3.12-2.99 (m, 1H), 2.95 (br t, J=6.5 Hz, 2H), 2.25-2.05 (m, 2H), 1.88-1.72 (m, 1H), 1.30-1.04 (m, 6H), 4H under the water peak; LCMS: [M+H]+=493.54.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(3-fluoro-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (22 mg, 0.049 mmol) and N,N-diisopropylethylamine (0.085 ml, 0.488 mmol) in DMF (1 mL) was added 2-bromoethyl methyl ether (0.014 mL, 0.147 mmol) afforded the title compound (20.1 mg, 77%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.66 (d, J=10.1 Hz, 1H), 7.52-7.42 (m, 3H), 7.36-7.30 (m, 1H), 7.29-7.21 (m, 1H), 6.33 (s, 2H), 4.46 (br s, 1H), 3.56-3.45 (m, 2H), 3.43-3.39 (m, 2H), 3.26 (s, 3H), 2.96 (s, 2H), 2.92-2.70 (m, 2H), 2.46-2.21 (m, 2H), 2.05-1.92 (m, 2H), 1.81-1.63 (m, 2H), 1.29-1.21 (m, 2H); LCMS: [M+H]+=509.56.
A procedure similar to Example 218 using 6-(2-amino-5-(2,3-difluoro-4-(piperidin-4-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (20.7 mg, 0.044 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL), 2 drops of AcOH (15.92 mg, 0.265 mmol) and NaBH(OAc)3 (56.2 mg, 0.265 mmol) afforded the title compound (11.8 mg, 50%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br s, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.54 (d, J=9.5 Hz, 1H), 7.48-7.41 (m, 2H), 7.25-7.18 (m, 1H), 7.16-7.10 (m, 1H), 6.43 (s, 2H), 4.52-4.43 (m, 1H), 3.42-3.39 (m, 2H), 3.00-2.92 (m, 2H), 2.76-2.65 (m, 2H), 2.36-2.30 (m, 2H), 2.02-1.93 (m, 2H), 1.70-1.60 (m, 2H), 0.98 (s, 3H), 0.97 (s, 3H); LCMS: [M +H]+=511.60.
A procedure similar to Example 218 using 6-(2-amino-5-(2,5-difluoro-4-(piperidin-4-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (23.8 mg, 0.051 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL) was added 2 drops of glacial AcOH (18.30 mg, 0.305 mmol) and NaBH(OAc)3 (64.6 mg, 0.305 mmol) afforded the title compound (24.4 mg, 89%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.53 (d, J=9.7 Hz, 1H), 7.45 (dd, J=1.3, 7.9 Hz, 1H), 7.43 (s, 1H), 7.40 (dd, J=11.7, 7.3 Hz, 1H), 7.27 (dd, J=11.6, 7.5 Hz, 1H), 6.39 (s, 2H), 4.53-4.43 (m, 1H), 3.39 (br dd, J=6.7, 2.6 Hz, 2H), 2.94 (t, J=6.5 Hz, 2H), 2.83-2.68 (m, 2H), 2.45-2.32 (m, 2H), 2.02-1.94 (m, 2H), 1.71-1.59 (m, 2H), 1.00 (s, 3H), 0.98 (s, 3H); LCMS: [M+H]+=511.60.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(2-methoxy-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (21.3 mg, 0.046 mmol) in CH2Cl2 (1.5 ml) and acetone (1.5 ml), 2 drops glacial AcOH(16.59 mg, 0.276 mmol) and NaBH(OAc)3 (58.6 mg, 0.276 mmol) afforded the title compound (14.1 mg, 61%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (brs, 1H), 7.89 (d, J=7.9 Hz, 1H), 7.43 (dd, J=7.9, 1.5 Hz, 1H), 7.41 (s, 1H), 7.39 (d, J=9.7 Hz, 1H), 7.14 (d, J=8.3 Hz, 1H), 6.61 (d, J=2.2 Hz, 1H), 6.59 (dd, J=8.4, 2.3 Hz, 1H), 6.14 (s, 2H), 4.42-4.35 (m, 1H), 3.73 (s, 3H), 3.42-3.38 (m, 2H), 2.94 (t, J=6.5 Hz, 2H), 2.72 (br d, J=6.5 Hz, 3H), 2.38-2.29 (m, 2H), 2.01-1.93 (m, 2H), 1.64-1.55 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H); LCMS: [M+H]+=505.58.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(3-methoxy-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (23.2 mg, 0.050 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 ml), glacial AcOH (18.07 mg, 0.301 mmol) and NaBH(OAc)3 (63.8 mg, 0.301 mmol) afforded the title compound (17.2 mg, 65%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.64 (d, J=10.1 Hz, 1H), 7.46 (dd, J=7.9, 1.6 Hz, 1H), 7.44 (s, 1H), 7.10 (s, 1H), 7.05-6.99 (m, 2H), 6.23 (s, 2H), 4.28-4.20 (m, 1H), 3.79 (s, 3H), 3.40 (br dd, J=6.5, 2.5 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.77-2.66 (m, 3H), 2.31-2.23 (m, 2H), 1.94-1.90 (m, 2H), 1.65-1.53 (m, 2H), 0.97 (s, 3H), 0.96 (s, 3H); LCMS: [M+H]+=505.58.
A procedure similar to Example 218 using (S)-6-(2-amino-6-fluoro-5-(4-(piperidin-3-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (16.5 mg, 0.038 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL), 2 drops glacial AcOH (13.75 mg, 0.229 mmol) and NaBH(OAc)3 (48.5 mg, 0.229 mmol) afforded the title compound (14.9 mg, 78%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.60 (d, J=10.3 Hz, 1H), 7.51-7.43 (m, 4H), 7.00 (br s, 2H), 6.23 (s, 2H), 4.49-4.30 (m, 1H), 3.44-3.38 (m, 2H), 3.14-2.99 (m, 1H), 2.99-2.93 (m, 2H), 2.85-2.61 (m, 2H), 2.33-2.12 (m, 1H), 2.11-1.98 (m, 1H), 1.82-1.68 (m, 1H), 1.66-1.46 (m, 1H), 1.43-1.29 (m, 1H), 0.99 (br s, 6H); LCMS: [M+H]+=475.56.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(7-(piperidin-4-yloxy)benzo[d][1,3]dioxol-4-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (22.5 mg, 0.047 mmol) in CH2Cl2 (1.5 ml) and acetone (1.5 mL), 2 drops of glacial AcOH (17.01 mg, 0.283 mmol) and NaBH(OAc)3 (60.0 mg, 0.283 mmol) afforded the title compound (13.7 mg, 53%) as a white powder 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=9.8 Hz, 1H), 7.45-7.38 (m, 2H), 6.92-6.84 (m, 1H), 6.68 (d, J=8.8 Hz, 1H), 6.30 (s, 2H), 5.99 (s, 2H), 4.42-4.34 (m, 1H), 3.41-3.38 (m, 2H), 2.94 (s, 2H), 2.75-2.65 (m, 3H), 2.34-2.23 (m, 2H), 1.96-1.90 (m, 2H), 1.64-1.54 (m, 2H), 0.97 (s, 3H), 0.96 (s, 3H); LCMS: [M+H]+=519.57.
Step 1: 3-(3-bromophenyl)-1-methoxy-1-oxopropan-2-aminium chloride:
To a stirred solution of 2-(3-bromophenyl)-1-carboxyethan-1-aminium chloride (10 g, 35.7 mmol) in MeOH (300 mL) was added dropwise SOCl2 (7.7 mL, 107.3 mmol) at 0° C. The reaction mixture was then heated at 100° C. for 16 h before cooling to RT. The solvent was removed under reduced pressure to afford the product (10.4 g, quantitative yield) as an off-white solid that was used in the next step without further purification. LCMS: [M+H]+=258.09.
Step 2: methyl 3-(3-bromophenyl)-2-(((trichloromethoxy)carbonyl)amino)propanoate:
To a stirred solution of 3-(3-bromophenyl)-1-methoxy-1-oxopropan-2-aminium chloride (10.5 g, 35.7 mmol) in 1, 4-dioxane (115 mL) was added trichloromethylchloroformate (5.9 mL, 50 mmol) at RT. The mixture was then heated at 100° C. for 16 h before cooling to RT. The solvent was concentrated under reduced pressure to afford crude product (14.8 g, quant) as a pale brown gummy liquid, which was used in the next step without purification. TLC: 10% MeOH: DCM; Rf=0.7.
Step 3: methyl 6-bromo-1-oxo-1,2,3,4-tetrahydroisoquinoline-3-carboxylate:
To a stirred solution of methyl 3-(3-bromophenyl)-2-(((trichloromethoxy)carbonyl)amino)propanoate (14.8 g, 35.5 mmol, quant) in CH2Cl2 (150 mL) was added AlCl3 (9.3 g, 71.1 mmol) portionwise at 0° C. The mixture was then heated at 45° C. for 3 h before cooling to RT, quenched with water (100 mL) and extracted with CH2Cl2 (2×300 mL). The combined organic layers were dried over Na2SO4 filtered and concentrated under reduced pressure to give crude residue. Purification by silica gel chromatography (100-200 mesh) using 0-60% EtOAc in hexane as an eluent afforded the product (2.9 g, 29%) as an off white solid. LCMS: [M+H]+=284.05.
Step 4: 6-bromo-3-(hydroxymethyl)-3,4-dihydroisoquinolin-1(2H)-one:
To a stirred solution of methyl 6-bromo-1-oxo-1,2,3,4-tetrahydroisoquinoline-3-carboxylate (2.9 g, 10.2 mmol) in EtOH (30 mL) was added portionwise LiBH4 (450 mg, 20.4 mmol) at 0° C. The reaction was stirred at RT for 16 h. The solvent was concentrated under reduced pressure to give crude residue, which was then diluted with water (100 mL) and extracted with CH2Cl2 (2×300 mL). The combined organic layers were dried over Na2SO4 and concentrated under reduced pressure to give crude residue. The crude compound was triturated with diethyl ether (100 mL), filtered and dried under vacuum to afford the product (2.5 g, 95%) as an off-white solid. LCMS: [M+H]+=256.03.
Step 5: (6-bromo-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl methanesulfonate:
To a stirred solution of 6-bromo-3-(hydroxymethyl)-3,4-dihydroisoquinolin-1(2H)-one (2.5 g, 9.8 mmol) in CH2Cl2 (30 mL) were added Et3N (2.6 mL, 19.6 mmol), methanesulfonylchloride (1.5 mL, 19.6 mmol) and DMAP (110 mg, 0.98 mmol) at 0° C. Then, the reaction mixture was stirred at RT for 16 h. The solvent was concentrated under reduced pressure to give crude residue. The crude compound was diluted with water (100 mL) and extracted with CH2Cl2 (2×300 mL). The combined organic layers were dried over Na2SO4 and concentrated under reduced pressure to give crude material which was triturated with diethyl ether (100 mL) filtered and dried under vacuum to afford the product (2.2 g, 68%) as an off-white solid. LCMS: [M+H]+=334.03.
Step 6: 6-bromo-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one:
To a stirred solution of 6-bromo-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl methanesulfonate (2.2 g, 6.6 mmol) in THF (25 mL) were added Et3N (13.5 mL, 99 mmol), 40% aqueous methylamine (13.5 mL) at RT in a sealed tube. The reaction was stirred at 100° C. for 16 h before cooling to RT. The solvent was concentrated under reduced pressure to give crude residue. The crude compound was purified by column chromatography (neutral alumina) using 0-5% MeOH in DCM as an eluent to afford the product (0.85 g, 48%) as an off white solid. 1H NMR (400 MHz, DMSO-d6): δ 7.82 (s, 1H), 7.74 (d, J=8.4 Hz, 1H), δ 7.53 (dd, J=10.4, 2.4 Hz, 2H), δ 3.68-3.60 (m, 1H), δ 2.99 (dd, J=16.0, 4.8 Hz, 1H), δ 2.88-2.78 (m, 1H), 2.61-2.52 (m, 2H), δ 2.27 (s, 3H); LCMS: [M+H]+=269.04.
Step 7: 6-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one
To a suspension of 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (85 mg, 0.223 mmol), 6-bromo-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.186 mmol), K3PO4 (118 mg, 0.557 mmol) and XPhos Pd G2 (17.54 mg, 0.022 mmol) was added a 5:1 mixture of butan-1-ol (4 ml): water (0.8 ml). The mixture was flushed with nitrogen and heated in the microwave at 90° C. for 2 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH, followed by reverse phase chromatography (C18, 0-100% ACN/H2O) to afford the title compound (30.6 mg, 37%) as beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.88 (br s, 1H), 7.84 (d, J=8.0 Hz, 1H), 7.78 (d, J=8.1 Hz, 1H), 7.65 (d, J=10.2 Hz, 1H), 7.62 (s, 1H), 7.47 (br d, J=8.1 Hz, 1H), 7.40-7.44 (m, 2H), 7.39 (s, 1H), 6.36 (s, 2H), 3.55-3.64 (m, 1H), 3.34 (td, J=6.4, 2.5 Hz, 3H), 2.94 (br dd, J=15.9, 4.5 Hz, 1H), 2.89 (br t, J=6.4 Hz, 2H), 2.77 (br dd, J=1 5.9, 9.3 Hz, 1H), 2.46-2.54 (m, 2H), 2.21 (s, 3H); LCMS: [M+H]+=446.67.
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (125 mg, 0.325 mmol), 1-(4-bromophenyl)-4-methyl-1,4-diazepane (73 mg, 0.271 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (19.84 mg, 0.027 mmol), aqueous K3PO4 (0.417 mL of a 1.3 M solution, 0.542 mmol), DME (4 mL) and H2O (2 mL) afforded the title compound (52 mg, 43%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.56 (d, J=10.1 Hz, 1H), 7.48-7.43 (m, 2H), 7.34 (d, J=7.8 Hz, 2H), 6.73 (d, J=8.9 Hz, 2H), 6.10 (s, 2H), 3.55-3.50 (m, 2H), 3.45 (t, J=6.2 Hz, 2H), 3.40 (td, J=6.6, 2.6 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.62-2.57 (m, 2H), 2.46-2.41 (m, 2H), 2.25 (s, 3H), 1.89 (quin, J=5.8 Hz, 2H); LCMS: [M+H]+=446.44.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (133 mg, 0.347 mmol) and 4-(4-bromophenyl)-1-isopropyl-2-methylpiperazine (86 mg, 0.289 mmol) [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (21.17 mg, 0.029 mmol), aqueous K3PO4 (0.445 mL of a 1.3 M solution, 0.579 mmol), DME (4 mL) and water (2 mL) afforded the title compound (67 mg, 48%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.57 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 2H), 7.38 (d, J=7.8 Hz, 2H), 6.95 (d, J=8.9 Hz, 2H), 6.16 (s, 2H), 3.58-3.47 (m, 2H), 3.40 (td, J=6.5, 2.6 Hz, 2H), 3.24-3.15 (m, 1H), 2.95 (t, J=6.5 Hz, 2H), 2.80 (dt, J=11.3, 3.0 Hz, 1H), 2.71 (td, J=11.0, 2.9 Hz, 1H), 2.65 (ddd, J=9.3, 6.2, 3.0 Hz, 1H), 2.45 (dd, J=11.2, 9.5 Hz, 1H), 2.35 (td, J=10.9, 2.8 Hz, 1H), 1.06 (dd, J=8.0, 6.5 Hz, 6H), 0.86 (d, J=6.5 Hz, 3H); LCMS: [M+H]+=474.30.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (139 mg, 0.363 mmol), 1-(4-bromophenyl)-4-isopropyl-1,4-diazepane (90 mg, 0.303 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (22.16 mg, 0.030 mmol), aqueous K3PO4 (0.466 mL of a 1.3 M solution, 0.606 mmol), DME (4 mL) and water (2 mL) afforded the title compound (30 mg, 21%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.56 (d, J=10.3 Hz, 1H), 7.46 (dd, J=7.9, 1.5 Hz, 1H), 7.44 (s, 1H), 7.33 (d, J=7.8 Hz, 2H), 6.72 (d, J=9.0 Hz, 2H), 6.09 (s, 2H), 3.49 (q, J=6.0 Hz, 4H), 3.40 (td, J=6.5, 2.7 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.85 (dt, J=13.1, 6.5 Hz, 1H), 2.70-2.64 (m, 2H), 2.45-2.41 (m, 2H), 1.79 (quin, J=5.9 Hz, 2H), 0.92 (d, J=6.6 Hz, 6H); LCMS: [M+H]+=474.43.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.303 mmol) and 2-(4-bromophenyl)octahydropyrrolo[1,2-a]pyrazine (71 mg, 0.252 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.48 mg, 0.025 mmol), aqueous K3PO4 (0.388 mL of a 1.3 M solution, 0.505 mmol), DME (4 mL) and water (2 mL) afforded the title compound (17 mg, 14%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.3 Hz, 1H), 7.48-7.42 (m, 2H), 7.39 (d, J=7.8 Hz, 2H), 6.98 (d, J=8.9 Hz, 2H), 6.16 (s, 2H), 3.81 (br d, J=9.8 Hz, 1H), 3.66 (br d, J=11.9 Hz, 1H), 3.08-2.98 (m, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.74 (td, J=11.7, 3.3 Hz, 1H), 2.40 (t, J=10.7 Hz, 1H), 2.22 (td, J=11.2, 3.1 Hz, 1H), 2.10-1.99 (m, 2H), 1.87-1.79 (m, 1H), 1.78-1.63 (m, 2H), 1.37 (qd, J=11.0, 6.8 Hz, 1H); LCMS: [M+H]+=458.63.
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (125 mg, 0.325 mmol) and 2-(4-bromophenyl)octahydro-1H-pyrrolo[1,2-a][1,4]diazepine (80 mg, 0.271 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (19.83 mg, 0.027 mmol), aqueous K3PO4 (0.417 mL of a 1.3 M solution, 0.542 mmol), DME (4 mL) and water (2 mL) afforded the title compound (64 mg, 50%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.55 (d, J=10.1 Hz, 1H), 7.49-7.43 (m, 2H), 7.33 (d, J=7.8 Hz, 2H), 6.73 (d, J=9.0 Hz, 2H), 6.09 (s, 2H), 3.81 (dd, J=13.9, 2.2 Hz, 1H), 3.66 (dt, J =14.5, 5.5 Hz, 1H), 3.52-3.44 (m, 1H), 2.98-2.88 (m, 5H), 2.48-2.44 (m, 1H), 2.24 (q, J =9.0 Hz, 1H), 2.04 (td, J=12.0, 4.2 Hz, 1H), 1.98-1.87 (m, 2H), 1.81-1.70 (m, 1H), 1.70-1.59 (m, 2H), 1.46-1.34 (m, 1H); LCMS: [M+H]+=472.40.
A procedure analogous to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (137 mg, 0.357 mmol) and (S)-1-(4-bromophenyl)-2,4-dimethylpiperazine (80 mg, 0.297 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (21.75 mg, 0.030 mmol), aqueous K3PO4 (0.457 mL of a 1.3 M solution, 0.594 mmol), DME (4 mL) and water (2 mL) afforded the title compound (57 mg 43%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.58 (d, J=10.3 Hz, 1H), 7.49-7.42 (m, 2H), 7.39 (d, J=7.9 Hz, 2H), 6.91 (d, J=8.9 Hz, 2H), 6.15 (s, 2H), 4.05-3.97 (m, 1H), 3.42-3.39 (m, 2H), 3.31-3.26 (m, 1H), 3.02-2.91 (m, 3H), 2.79 (br dd, J=10.8, 1.3 Hz, 1H), 2.63 (br d, J=10.8 Hz, 1H), 2.24 (dd, J=10.9, 3.5 Hz, 1H), 2.19 (s, 3H), 2.04 (td, J=11.1, 3.4 Hz, 1H), 1.02 (d, J=6.5 Hz, 3H); LCMS: [M+H]+=446.44.
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (117 mg, 0.305 mmol), 1-(4-bromophenyl)-N,N-dimethylpiperidin-4-amine (72 mg, 0.254 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.60 mg, 0.025 mmol), aqueous K3PO4 (0.391 mL of a 1.3 M solution, 0.508 mmol), DME (4 mL) and water (2 mL) afforded the title compound (46 mg, 39%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 2H), 7.38 (d, J=7.8 Hz, 2H), 6.97 (d, J=8.9 Hz, 2H), 6.16 (s, 2H), 3.74 (br d, J=12.6 Hz, 2H), 3.40 (td, J=6.5, 2.7 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.72-2.64 (m, 2H), 2.23-2.20 (m, 1H), 2.18 (s, 6H), 1.82 (br d, J=12.0 Hz, 2H), 1.45 (qd, J=11.9, 3.9 Hz, 2H); LCMS: [M+H]+=460.51.
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.302 mmol), tert-butyl-4-(4-bromophenyl)-cis-2,6-dimethylpiperazine-1-carboxylate (93 mg, 0.252 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.43 mg, 0.025 mmol), aqueous K3PO4 (0.387 mL of a 1.3 M solution, 0.504 mmol), DME (4 mL) and water (2 mL) afforded the product (79 mg, 58%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.48-7.39 (m, 4H), 6.99 (d, J=8.9 Hz, 2H), 6.18 (s, 2H), 4.15-4.08 (m, 2H), 3.54 (br d, J=12.2 Hz, 2H), 3.40 (td, J=6.5, 2.7 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.81 (dd, J=12.2, 4.2 Hz, 2H), 1.43 (s, 9H), 1.25 (d, J=6.8 Hz, 6H); LCMS: [M+H]+=546.58.
Step 1: tert-butyl ((6-bromo-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(mehyl)carbamate
To a solution of 6-bromo-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one (200 mg, 0.743 mmol) in CH2Cl2 (6 mL) at RT was added di-tert-butyl dicarbonate (178 mg, 0.817 mmol) solid in one portion, followed by N,N-diisopropylethylamine (0.272 ml, 1.561 mmol) and the reaction mixture was stirred at RT. After 1 h the reaction mixture was concentrated onto Celite®. Silica gel chromatography (eluting with 0-20% EtoAc/Hexanes) afforded the product as a colourless oil (359 mg, 82%). LCMS: [M+H]+=369.45.
Step 2: tert-butyl methyl((1-oxo-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)carbamate
A microwave vial containing a mixture of tert-butyl ((6-bromo-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate (75 mg, 0.203 mmol), bis(pinacolato)diboron (58.3 mg, 0.230 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (14.86 mg, 0.020 mmol) and KOAc (59.8 mg, 0.609 mmol) in 1,4-dioxane (5 mL) was flushed with Ar. The vial was sealed and heated at 100° C. in a microwave reactor for 1 h. The resulting material was concentrated to obtain the crude product, which was taken to the next step without further purification. (84 mg, quantitative yield assuming100% conversion). LCMS: [M+H]+=417.65.
Step 3: tert-butyl ((6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate
A procedure analogous to that of Example 47, Step 3 was used, coupling 5-bromo-6-fluoro-3-iodopyridin-2-amine (75 mg, 0.237 mmol) with tert-butyl methyl((1-oxo-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)carbamate (84 mg, 0.202 mmol) with Na2CO3 monohydrate (61.8 mg, 0.498 mmol) and bis(triphenylphosphine)palladium(II) dichloride (19.99 mg, 0.028 mmol), in CH3CN (3.5 mL) and water (1.3 mL) under microwave reaction conditions at 80° C. for 1 h. The reaction mixture was concentrated onto Celite®. Silica gel chromatography (eluting with 0-60% EtOAc/hexanes) afforded the product as a beige solid (89 mg, 78%). LCMS: [M+H]+=479.57.
Step 4: tert-butyl ((6-(2-amino-6-fluoro-5-(4-morpholinophenyl)pyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate
A procedure similar to Example 43, Step 5 using tert-butyl ((6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate (42 mg, 0.088 mmol) with 4-(morpholino)phenylboronic acid (25.4 mg, 0.123 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (9.62 mg, 0.013 mmol) and Cs2CO3 (86 mg, 0.263 mmol) in DME (4 mL) and water (1 mL). The reaction mixture was concentrated onto Celite®. Silica gel chromatography (eluting with 0-80% EtOAc/Hex) afforded the product (38 mg, 73%) as a pale yellow solid LCMS: [M+H]+=562.68.
Step 5: 6-(2-amino-6-fluoro-5-(4-morpholinophenyl)pyridin-3-yl)-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one
A solution of tert-butyl ((6-(2-amino-6-fluoro-5-(4-morpholinophenyl)pyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate (37 mg, 0.066 mmol) with TFA (0.050 mL, 0.659 mmol) was stirred in CH2Cl2 (1.5 mL) at RT for 1 h. The reaction mixture was concentrated and the methanol solution of the residue was passed through a Waters PoraPak CX cartridge to afford the title compound as a beige powder (27.5 mg, 25% based on purity of 86%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-7.9 (m, 1H), 7.7-7.8 (m, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 1H), 7.4-7.4 (m, 3H), 6.9-7.0 (m, 2H), 6.1-6.3 (m, 2H), 3.7-3.8 (m, 4H), 3.7-3.7 (m, 1H), 3.1-3.2 (m, 4H), 3.0-3.1 (m, 1H), 2.8-2.9 (m, 1H), 2.6-2.7 (m, 2H), 2.3-2.4 (m, 3H); LCMS: [M+H]+=462.71.
Step 1: tert-butyl ((6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate
A procedure analogous to Example 43, Step 5 using tert-butyl ((6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3 yl)methyl)(methyl)carbamate (41 mg, 0.086 mmol) 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (39.5 mg, 0.120 mmol) gave the product as a brown solid (42 mg, 73% based on purity of 90%) LCMS: [M+H]+=603.85.
Step 2: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one
A solution of tert-butyl ((6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate (42 mg, 0.070 mmol) in CH2Cl2 (1.5 mL) at RT was stirred with TFA (0.053 mL, 0.697 mmol) for 1 h. The reaction mixture was concentrated onto Celite®. Silica gel chromatography (eluting with DCM containing 0-5% MeOH and 0-0.5% NH4OH), and the resulting crude product was passed through a Waters PoraPak CX cartridge to afford the title compound as a beige solid (23 mg, 62%). 1H NMR (500 MHz, DMSO-d6) δ 7.9-7.9 (m, 1H), 7.7-7.7 (m, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 1H), 7.4-7.4 (m, 3H), 6.9-7.0 (m, 2H), 6.1-6.2 (m, 2H), 3.7-3.7 (m, 1H), 3.1-3.2 (m, 4H), 3.0-3.1 (m, 1H), 2.8-2.9 (m, 1H), 2.7-2.7 (m, 1H), 2.6-2.6 (m, 6H), 2.3-2.3 (m, 3H), 1.0-1.0 (m, 6H); LCMS: [M+H]+=503.64.
Step 1: tert-butyl 6-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinoline-2(1H)-carboxylate
To a suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (208 mg, 0.587 mmol), tert-butyl 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinoline-2(1 H)-carboxylate (232 mg, 0.646 mmol) and Cs2CO3 (478 mg, 1.47 mmol) in a mixture of DME (4 mL) and water (1.3 mL) was added PdCl2dppf (43.0 mg, 0.059 mmol). The mixture was flushed with nitrogen heated in the microwave at 90° C. for 2.5 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography, eluting with 0-10% MeOH/DCM+1% NH4OH to afford the product (278 mg, 93%) as a beige solid. LCMS: [M+H]+=507.65.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 6-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinoline-2(1 H)-carboxylate (278 mg, 0.549 mmol) in CH2Cl2 (5.0 mL) was added TFA (1.26 mL, 16.46 mmol). The reaction was stirred at RT for 3 d. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the title compound (226 mg, 79%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.81-8.96 (m, 1H), 8.13 (br s, 1H), 7.67 (d, J=10.2 Hz, 1H), 7.63 (d, J=10.0 Hz, 1H), 7.39-7.47 (m, 3H), 7.26 (d, J=8.0 Hz, 1H), 6.37 (s, 2H), 4.28 (s, 2H), 3.37-3.46 (m, 4H), 3.02 (s, 2H), 2.90-2.97 (m, 2H); LCMS: [M+H]+=407.55.
A procedure similar to Example 218 using 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (16.5 mg, 0.041 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 mL), 2 drops of glacial AcOH (14.70 mg, 0.245 mmol) and NaBH(OAc)3 (51.9 mg, 0.245 mmol) afforded the title compound (6.8 mg, 36%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (brs, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.50-7.42 (m, 4H), 6.88 (d, J=8.7 Hz, 2H), 6.23 (s, 2H), 4.79-4.70 (m, 1H), 3.74-3.67 (m, 2H), 3.44-3.38 (m, 2H), 2.99-2.90 (m, 4H), 2.35-2.28 (m, 1H), 0.88 (s, 3H), 0.87 (s, 3H); LCMS: [M+H]+=447.64.
A procedure similar to Example 177, Step 1 using tert-butyl 4-(2-cyano-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)piperidine-1-carboxylate (420 mg, 0.981 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (289 mg, 0.817 mmol), 1,4-dioxane (3 mL) Cs2CO3 (799 mg, 2.451 mmol), water (1 mL) and Pd(dppf)Cl2 (90 mg, 0.123 mmol) afforded the product (470 mg, 100%) as a beige powder which was used for the next step. LCMS: [M+H]+=576.60.
Step 2: 5-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-cyanophenoxy)piperidine-1-carboxylate (470 mg, 0.817 mmol), CH2Cl2 (4 mL) and TFA (2 mL, 26.1 mmol) afforded the title compound (298.2 mg, 73%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.88 (d, J=2.2 Hz, 1H), 7.78 (br d, J=9.0 Hz, 1H), 7.72 (d, J=10.3 Hz, 1H), 7.61 (d, J=10.0 Hz, 1H), 7.43 (d, J=7.1 Hz, 1H), 7.35 (d, J=9.2 Hz, 1H), 6.37 (s, 2H), 4.73-4.64 (m, 1H), 3.43-3.38 (m, 2H), 2.93 (br d, J=6.6 Hz, 5H), 2.63-2.56 (m, 2H), 1.95-1.88 (m, 2H), 1.58-1.48 (m, 2H); LCMS: [M+H]+=476.54.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(3-fluoro-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24 mg, 0.053 mmol) and N,N-diisopropylethylamine (0.046 mL, 0.266 mmol) in CH2Cl2 (3 ml) and acetic anhydride (5.04 μl, 0.053 mmol) afforded the title compound (21.7 mg, 79%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.66 (d, J=10.1 Hz, 1H), 7.50-7.43 (m, 3H), 7.36-7.25 (m, 2H), 6.33 (s, 2H), 4.70-4.60 (m, 1H), 3.87-3.78 (m, 1H), 3.71-3.62 (m, 1H), 3.40 (dt, J=6.5, 2.6 Hz, 2H), 3.38-3.34 (m, 1H), 3.25 (ddd, J=13.0, 8.9, 3.5 Hz, 1H), 3.00-2.91 (m, 2H), 2.02 (s, 3H), 2.00-1.94 (m, 1H), 1.93-1.86 (m, 1H), 1.71-1.61 (m, 1H), 1.59-1.49 (m, 1H); LCMS: [M+H]+=493.46.
A procedure similar to Example 218 using 5-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (23.8 mg, 0.050 mmol) and N,N-diisopropylethylamine (0.044 mL, 0.250 mmol) in CH2Cl2 (3 mL) and acetic anhydride (4.73 μl, 0.050 mmol) afforded the title compound (20.6 mg, 76%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.91 (d, J=2.2 Hz, 1H), 7.82 (br d, J=8.9 Hz, 1H), 7.73 (d, J=10.3 Hz, 1H), 7.61 (d, J=10.0 Hz, 1H), 7.46-7.37 (m, 2H), 6.38 (s, 2H), 4.95-4.85 (m, 1H), 3.73-3.60 (m, 2H), 3.49-3.38 (m, 4H), 2.97-2.89 (m, 2H), 2.03 (s, 3H), 2.02-1.95 (m, 1H), 1.93-1.85 (m, 1H), 1.77-1.67 (m, 1H), 1.65-1.56 (m, 1H); LCMS: [M+H]+=518.52.
A procedure similar to Example 218 using 5-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (24 mg, 0.050 mmol) in CH2Cl2 (1.5 mL) and acetone (1.5 ml), 2 drops of glacial AcOH (18.19 mg, 0.303 mmol) and NaBH(OAc)3 (64.2 mg, 0.303 mmol) afforded the title compound (16.3 mg, 59%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.88 (d, J=2.2 Hz, 1H), 7.79 (br d, J=9.0 Hz, 1H), 7.72 (d, J=10.3 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.43 (d, J=7.1 Hz, 1H), 7.34 (d, J=9.2 Hz, 1H), 6.37 (s, 2H), 4.69-4.61 (m, 1H), 3.44-3.37 (m, 2H), 2.96-2.89 (m, 2H), 2.74-2.66 (m, 3H), 2.41-2.33 (m, 2H), 1.98-1.89 (m, 2H), 1.74-1.62 (m, 2H), 0.98 (s, 3H), 0.97 (s, 3H); LCMS: [M+H]+=477.44.
A procedure similar to Example 186 using 5-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (23.8 mg, 0.050 mmol) and N,N-diisopropylethylamine (0.087 mL, 0.501 mmol) in DMF (1 mL) and 2,2,2-trifluoroethyl trifluoromethanesulfonate (23.23 mg, 0.100 mmol) afforded the title compound (20.4 mg, 69%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.89 (d, J=2.2 Hz, 1H), 7.79 (br d, J=9.0 Hz, 1H), 7.72 (d, J=10.3 Hz, 1H), 7.61 (d, J=10.1 Hz, 1H), 7.43 (d, J=7.0 Hz, 1H), 7.36 (d, J=9.0 Hz, 1H), 6.38 (s, 2H), 4.74-4.65 (m, 1H), 3.44-3.37 (m, 2H), 3.21 (q, J=10.2 Hz, 2H), 2.95-2.90 (m, 2H), 2.89-2.82 (m, 2H), 2.67-2.59 (m, 2H), 1.99-1.90 (m, 2H), 1.77-1.67 (m, 2H); LCMS: [M+H]+=558.54.
A solution of 4.0 M hydrogen chloride in dioxane (1.18 mL, 4.73 mmol) was added to a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-4-methoxypiperidine-1-carboxylate (115 mg, 0.189 mmol), in MeOH (4 mL), and the reaction mixture was stirred at RT for 2.5 h. The reaction mixture was concentrated, dissolved in MeOH and passed through a Waters PoraPak CX column to afford 84 mg of the intermediate as a cream coloured solid. This intermediate was taken up in CH2Cl2 (5 mL) with propan-2-one (0.013 ml, 0.179 mmol) and glacial AcOH (2.56 μl, 0.045 mmol). NaBH(OAC)3 (57.0 mg, 0.269 mmol) was added and the mixture was stirred overnight at RT and partitioned between saturated NaHCO3 solution and CH2Cl2. The layers were separated and the aqueous layer was further extracted with CH2Cl2 (x 2). The combined extract was concentrated onto Celite®. Silica gel chromatography (eluting with CH2Cl2 containing 0-3% MeOH and 0-0.3% NH4OH) afforded the title compound as a pale yellow powder (23 mg, 50%). 1H NMR (500 MHz, DMSO-d6) δ 8.00-7.92 (m, 1H), 7.92-7.86 (m, 1H), 7.72-7.62 (m, 1H), 7.59-7.52 (m, 2H), 7.50-7.47 (m, 1H), 7.47-7.45 (m, 1H), 7.44-7.38 (m, 2H), 6.32 (s, 2H), 3.45-3.40 (m, 2H), 3.02-2.93 (m, 2H), 2.93-2.84 (m, 3H), 2.73-2.65 (m, 1H), 2.63-2.56 (m, 2H), 2.49-2.43 (m, 2H), 2.00-1.91 (m, 2H), 1.90-1.75 (m, 2H), 1.07-0.93 (m, 6H); LCMS: [M+H]+=489.73
Step 1: 1-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1,2,3,6-tetrahydropyridine
A procedure analogous to Example 47, Step 2 using a mixture of 4-(4-bromophenyl)-1-isopropyl-1,2,3,6-tetrahydropyridine (150 mg, 0.535 mmol), bis(pinacolato)diboron (154 mg, 0.605 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (39.2 mg, 0.054 mmol) and KOAc (158 mg, 1.61 mmol) in 1,4-dioxane (6 mL) afforded the product (175 mg, 100%) which was dissolved in DME to make 10 mL stock solution with a concentrations of 17.5 mg/mL which was used in the next step. LCMS: [M+H]+=328.64 (stock solution).
Step 2: 6-(2-Amino-6-fluoro-5-(4-(1-isopropyl-1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 43, Step 5 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol), 1-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1,2,3,6-tetrahydropyridine (2.40 mL of a stock solution prepared in Step 1, 0.128 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (10.85 mg, 0.015 mmol), Cs2CO3 (97 mg, 0.296 mmol) and DME (2.5 mL) afforded the title compound as a pale yellowish brown solid (16 mg, 32%)1H NMR (500 MHz, DMSO-d6) δ 8.16-8.07 (m, 1H), 7.69-7.64 (m, 1H), 7.63-7.59 (m, 1H), 7.55-7.39 (m, 5H), 6.40-6.26 (m, 2H), 6.25-6.16 (m, 1H), 3.44-3.40 (m, 2H), 3.22-3.14 (m, 2H), 2.97-2.89 (m, 2H), 2.85-2.74 (m, 1H), 2.72-2.61 (m, 2H), 2.49-2.43 (m, 2H), 1.13-0.96 (m, 6H); LCMS: [M+H]+=475.62
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-3,6-dihydropyridine-1(2H)-carboxylate
A mixture of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (200 mg, 0.565 mmol), (4-(1-(tert-Butoxycarbonyl)-1,2,3,6-tetrahydropyridin-4-yl)phenyl)boronic acid pinacol ester (261 mg, 0.678 mmol), [1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II) (62.0 mg, 0.085 mmol), and Cs2CO3 (552 mg, 1.694 mmol) in DME (8 ml) was flushed with argon. Water (3 ml) was added and the rxn mix was heated in a microwave reactor (high absorbance) at 90C for 1.75 h. The mixture was mixed with brine and EA, the org phase was separated, aq phase was extracted with EA, the combined org phase was dried over Na2SO4 and concentrated onto celite. Purification by sgc eluting with hexanes containing 0-100% EtOAc afforded the product as an off white solid (226 mg, 75% yield). LCMS: [M+H]+=533.72.
Step 2: 6-(2-amino-5-(4-(1-ethyl-1,2,3,6-tetrahydropyridin-4-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-3,6-dihydropyridine-1(2H)-carboxylate (226 mg, 0.424 mmol) was stirred at RT with DCM (3 mL) and TFA (1 mL) for 1 h. Complete conversion to the desired product was observed. The mixture was concentrated to dryness, passed through a porapak cation exchange cartridge to isolate the desired product as an off white solid (176 mg). LCMS: [M+H]+=433.57.
Step 3: 6-(2-amino-5-(4-(1-ethyl-1,2,3,6-tetrahydropyridin-4-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a slurry of 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.104 mmol) in N,N-Dimethylformamide (DMF) (2 ml) at RT, was added 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1 (2H)-one (45 mg, 0.104 mmol) and Bromoethane (0.023 ml, 0.312 mmol) and the rxn mixture was stirred o/n at RT. The volatiles (boromoethane and TEA) were removed on a rotavap. The DMF solution was diluted with 5 mL of water, the solid was filtered, the filter cake was washed with water (x2) and dried under high vac to isolate the title compound as a pale yellow solid (24 mg, 95% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.16-8.06 (m, 1H), 7.70-7.64 (m, 1H), 7.64-7.59 (m, 1H), 7.57-7.38 (m, 5H), 6.38-6.26 (m, 2H), 6.25-6.17 (m, 1H), 3.50-3.39 (m, 4H), 3.16-3.02 (m, 2H), 2.95-2.88 (m, 2H), 2.68-2.59 (m, 2H), 2.47-2.40 (m, 2H), 1.13-0.97 (m, 3H); LCMS: [M+H]+=461.58.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(4-(1,2,3,6-tetrahydropyridin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1 (2H)-one (40 mg, 0.092 mmol) and (bromomethyl)cyclopropane (62.4 mg, 0.462 mmol) afforded the title compound (17 mg, 36%) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99-7.93 (m, 1H), 7.56-7.50 (m, 1H), 7.49-7.45 (m, 1H), 7.40-7.30 (m, 4H), 7.29 (br d, J=7.0 Hz, 1H), 6.25-6.13 (m, 2H), 6.12-6.02 (m, 1H), 3.33-3.25 (m, 4H), 3.10-2.98 (m, 2H), 2.82-2.76 (m, 2H), 2.69-2.52 (m, 2H), 2.32-2.13 (m, 2H), 0.76 (br s, 1H), 0.46-0.28 (m, 2H), 0.09-0.15 (m, 2H); LCMS: [M+H]+=487.66.
Step 1: tert-butyl ((6-(2-amino-5-(4-chlorophenyl)-6-fluoropyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate
A procedure analogous to Example 177, Step 1 using tert-butyl ((6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate (48 mg, 0.100 mmol), 4-chlorophenylboronic acid, pinacol ester (33.4 mg, 0.140 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (10.99 mg, 0.015 mmol) and Cs2CO3 (98 mg, 0.300 mmol) in DME (4 mL) and water (1 mL) afforded the title compound as an off white solid (42.5 mg, 83%). LCMS: [M+H]+=511.54.
Step 2: 6-(2-amino-5-(4-chlorophenyl)-6-fluoropyridin-3-yl)-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one
A solution of tert-butyl ((6-(2-amino-5-(4-chlorophenyl)-6-fluoropyridin-3-yl)-1-oxo-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)(methyl)carbamate (42.5 mg, 0.083 mmol) in DCM (1.5 mL) and TFA (0.053 mL, 0.697 mmol) in CH2Cl2 (1.5 mL) was stirred at RT with for 1 h. The reaction mixture was concentrated to dryness and the methanol solution of the residue was passed through a Waters PoraPak CX cartridge to afford the title compound as an off-white solid (26 mg, 72%). 1H NMR (500 MHz, DMSO-d6) δ 7.94-7.88 (m, 1H), 7.81-7.74 (m, 1H), 7.71-7.65 (m, 1H), 7.64-7.55 (m, 2H), 7.53-7.40 (m, 4H), 6.44-6.34 (m, 2H), 3.72 (br s, 1H), 3.09-3.01 (m, 1H), 2.91-2.82 (m, 1H), 2.70-2.63 (m, 2H), 2.33 (s, 3H); LCMS: [M+H]+=411.44.
To a solution of 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.048 mmol) and N,N-diisopropylethylamine (0.084 ml, 0.480 mmol) in DMF (1.0 mL) was added 2-iodopropane (40.8 mg, 0.240 mmol). The reaction was stirred at RT for 16 h, concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH, followed by reverse phase chromatography (C18, 0-100% ACN/H2O) to afford the title compound (12.2 mg, 57%) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.62 (dd, J=10.1, 2.63 Hz, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.23-7.30 (m, 2H), 7.10 (d, J=8.0 Hz, 1H), 6.27 (s, 2H), 3.64 (s, 2H), 3.40-3.43 (m, 2H), 2.93 (br t, J=6.3 Hz, 2H), 2.83-2.89 (m, 1H), 2.81 (br t, J=5.5 Hz, 2H), 2.67-2.73 (m, 2H), 1.06 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=449.61.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.048 mmol) and N,N-diisopropylethylamine (0.084 ml, 0.480 mmol) in DMF (1.0 mL) and 2-bromoethyl methyl ether (33.4 mg, 0.240 mmol) afforded the title compound (14.5 mg, 65%) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.62 (dd, J=10.1, 4.7 Hz, 2H), 7.43 (d, J=7.1 Hz, 1H), 7.23-7.32 (m, 2H), 7.08 (d, J=8.0 Hz, 1H), 6.28 (s, 2H), 3.61 (s, 2H), 3.53 (t, J=5.9 Hz, 2H), 3.41 (td, J=6.5, 2.6 Hz, 2H), 3.27 (s, 3H), 2.93 (br t, J=6.4 Hz, 2H), 2.80-2.85 (m, 2H), 2.69-2.74 (m, 2H), 2.65 (t, J=5.8 Hz, 2H); LCMS: [M+H]+=465.60.
Step 1: (1R,5S)-3-isopropyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane
A mixture of (1R,5S)-1-(4-bromophenyl)-3-isopropyl-3-azabicyclo[3.1.0]hexane (235 mg, 0.839 mmol), bis(pinacolato)diboron (241 mg, 0.948 mmol, preparation described in Example 110, Step 1), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (61.4 mg, 0.084 mmol) and KOAc (247 mg, 2.52 mmol) in 1,4-dioxane (10 mL), was flushed with argon. The reaction mixture was heated at 100° C. in a microwave reactor for 1 h. The reaction mixture was filtered through a thin pad of Celite®. The filtrate was concentrated to dryness, and the residue was dissolved in DME to make a 10 mL stock solution with the concentration estimated to be 27.4 mg/mL. LCMS: [M+H]+=328.70.
Step 2: 8-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a 100 mL round bottom flask charged with 6-bromo-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (896 mg, 3.67 mmol), bis(pinacolato)diboron (1025 mg, 4.04 mmol) and KOAc (1081 mg, 11.01 mmol), was added anhydrous 1,4-dioxane (40.0 ml). The system was flushed with nitrogen then PdCl2dppf (269 mg, 0.367 mmol) was added. The mixture was further flushed with nitrogen then heated at 100° C. overnight. LCMS analysis showed a 58:42 mixture of the boronic acid and boronate. The reaction was diluted with acetonitrile, filtered through a pad of Celite, and concentrated in vacuo. The crude material was used without further purification in the next step. Boronate LCMS: [M+H]+=292.34, boronic acid LCMS: [M+H]+=210.39.
Step 3: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of 5-bromo-6-fluoro-3-iodopyridin-2-amine (1280 mg, 4.04 mmol) and 8-fluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (1069 mg, 3.67 mmol) in CH3CN (15 mL), in a microwave vial, was added a solution of Na2CO3—H2O (956 mg, 7.71 mmol) in water (3.75 mL). The system was flushed with nitrogen and bis(triphenylphosphine)palladium(II) dichloride (309 mg, 0.441 mmol) was added. The reaction was further flushed with nitrogen then heated in the microwave at 80° C. for 3 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-100% EtOAc/Hexanes to give the product (872 mg, 67%) as a beige solid. LCMS: [M+H]+=354.40.
Step 4: 6-(2-amino-6-fluoro-5-(4-((1R,5S)-3-isopropyl-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 177, Step 1 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.113 mmol), (1R,5S)-3-isopropyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (1.75 mL, 0.147 mmol, 27.5 mg/ml in ACN) and Cs2CO3 (92 mg, 0.282 mmol) in DME (2 mL) and water (0.67 mL), and PdCl2dppf (8.26 mg, 0.011 mmol) afforded the title compound (26.9 mg, 50%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) b 8.00 (br s, 1H), 7.66 (d, J=10.2 Hz, 1H), 7.47 (d, J=7.2 Hz, 2H), 7.30 (s, 1H), 7.26 (br d, J=12.0 Hz, 1H), 7.20 (d, J=8.4 Hz, 2H), 6.40 (s, 2H), 3.05 (d, J=8.6 Hz, 1H), 2.94 (br t, J=6.3 Hz, 2H), 2.57 (d, J=8.4 Hz, 1H), 2.43-2.49 (m, 2H), 1.81 (dt, J=7.8, 3.8 Hz, 1H), 1.31 (t, J=3.9 Hz, 1H), 1.04 (d, J=6.4 Hz, 3H), 1.01 (d, J=6.2 Hz, 3H), 0.74 (dd, J=7.9, 3.6 Hz, 1H); LCMS: [M+H]+=475.62.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(8-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate
To a suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (130 mg, 0.367 mmol), 4-(N-Boc-piperidino)phenylboronic acid pinacol ester (171 mg, 0.440 mmol) and Cs2CO3 (299 mg, 0.918 mmol) in DME (5 mL) and water (1.67 mL) was added PdCl2dppf (26.9 mg, 0.037 mmol). The mixture was flushed with nitrogen and heated in the microwave at 90° C. for 2.5 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography (eluting with 0-10% MeOH/DCM+1% NH4OH) to afford the product (196 mg, 100%) as a brown solid. LCMS: [M+H]+=535.67.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(8-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate (196 mg, 0.367 mmol) in CH2Cl2 (5.0 ml) was added TFA (0.84 mL, 11.0 mmol). The reaction was stirred at RT for 72 h then concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the product as the TFA salt (154 mg, 77%) as beige solid. LCMS: [M+H]+=435.53.
Step 3: 6-(2-amino-6-fluoro-5-(4-(1-isopropylpiperidin-4-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (29 mg, 0.053 mmol) and N,N-diisopropylethylamine (0.092 ml, 0.529 mmol) in DMF (1.0 mL) was added 2-iodopropane (44.9 mg, 0.264 mmol). The reaction was stirred at RT for 96 h, then concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH to give the title compound (11.9 mg, 47%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99 (br s, 1H), 7.67 (d, J=10.2 Hz, 1H), 7.48 (d, J=7.3 Hz, 2H), 7.24-7.33 (m, 4H), 6.39 (s, 2H), 2.94 (br t, J=6.3 Hz, 2H), 2.89 (br d, J=11.4 Hz, 2H), 2.71 (dt, J=13.1, 6.6 Hz, 1H), 2.44-2.49 (m, 1H), 2.18-2.26 (m, 2H), 1.77 (br d, J=12.8 Hz, 2H), 1.62 (qd, J=12.3, 3.5 Hz, 2H), 1.24 (s, 1H), 1.00 (d, J=6.60 Hz, 6H); LCMS: [M+H]+=477.63.
A procedure similar to Example 186 using 5-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(piperidin-4-yloxy)benzonitrile (26 mg, 0.055 mmol) and N,N-diisopropylethylamine (0.095 mL, 0.547 mmol) in DMF (1 mL) and 2-bromoethyl methyl ether (0.015 mL, 0.164 mmol) afforded the title compound (21.4 mg, 70%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.89 (d, J=2.0 Hz, 1H), 7.79 (br d, J=8.9 Hz, 1H), 7.72 (d, J=10.3 Hz, 1H), 7.61 (d, J=10.0 Hz, 1H), 7.43 (d, J=7.1 Hz, 1H), 7.35 (d, J=9.0 Hz, 1H), 6.38 (s, 2H), 4.73-4.62 (m, 1H), 3.52-3.43 (m, 2H), 3.41 (dt, J=6.6, 2.9 Hz, 2H), 3.24 (s, 3H), 2.92 (br t, J=6.5 Hz, 2H), 2.83-2.61 (m, 2H), 2.60-2.51 (m, 2H), 2.44-2.25 (m, 2H), 2.03-1.89 (m, 2H), 1.79-1.64 (m, 2H); LCMS: [M +H]+=534.62.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(2-methoxy-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (25 mg, 0.054 mmol) and N,N-diisopropylethylamine (0.047 ml, 0.270 mmol) in CH2Cl2 (3 mL) and acetic anhydride (5.11 μl, 0.054 mmol) afforded the title compound (19.6 mg, 68%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.92 (br s, 1H), 7.89 (d, J=7.8 Hz, 1H), 7.45-7.37 (m, 3H), 7.16 (d, J=8.1 Hz, 1H), 6.67-6.61 (m, 2H), 6.14 (s, 2H), 4.72-4.62 (m, 1H), 3.89-3.81 (m, 1H), 3.74 (s, 3H), 3.72-3.64 (m, 1H), 3.42-3.37 (m, 2H), 3.36-3.34 (m, 1H), 3.29-3.22 (m, 1H), 2.94 (s, 2H), 2.02 (s, 3H), 2.00-1.94 (m, 1H), 1.93-1.86 (m, 1H), 1.68-1.59 (m, 1H), 1.55-1.48 (m, 1H); LCMS: [M+H]+=505.50.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(3-methoxy-4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (25 mg, 0.054 mmol) and N,N-diisopropylethylamine (0.047 mL, 0.270 mmol) in CH2Cl2 (3 mL) and acetic anhydride 5.11 μl, 0.054 mmol) afforded the title compound (22.8 mg, 79%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.65 (d, J=10.1 Hz, 1H), 7.49-7.45 (m, 1H), 7.44 (s, 1H), 7.13 (s, 1H), 7.10-7.03 (m, 2H), 6.24 (s, 2H), 4.56-4.48 (m, 1H), 3.87-3.81 (m, 1H), 3.80 (s, 3H), 3.71-3.64 (m, 1H), 3.44-3.38 (m, 2H), 3.31-3.28 (m, 1H), 3.27-3.20 (m, 1H), 2.95 (t, J=6.5 Hz, 2H), 2.01 (s, 3H), 1.96-1.89 (m, 1H), 1.88-1.81 (m, 1H), 1.67-1.58 (m, 1H), 1.56-1.46 (m, 1H); LCMS: [M+H]+=505.50.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(7-(piperidin-4-yloxy)benzo[d][1,3]dioxol-4-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (20.1 mg, 0.042 mmol) and N,N-diisopropylethylamine (0.037 ml, 0.211 mmol) in CH2Cl2 (3 mL) and acetic anhydride (3.99 μL, 0.042 mmol) afforded the title compound (18.6 mg, 81%) as a yellow powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.59 (d, J=9.8 Hz, 1H), 7.44-7.39 (m, 2H), 6.90 (d, J=7.8 Hz, 1H), 6.74 (d, J=8.9 Hz, 1H), 6.30 (s, 2H), 6.00 (s, 2H), 4.68-4.60 (m, 1H), 3.83-3.76 (m, 1H), 3.70-3.61 (m, 1H), 3.43-3.37 (m, 2H), 3.36-3.34 (m, 1H), 3.28-3.23 (m, 1H), 2.98-2.91 (m, 2H), 2.01 (s, 3H), 1.99-1.91 (m, 1H), 1.90-1.82 (m, 1H), 1.68-1.59 (m, 1H), 1.57-1.48 (m, 1H); LCMS: [M+H]+=519.57.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(8-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate
A procedure similar to Example 177 Step 1 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (130 mg, 0.367 mmol), tert-butyl 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]tetrahydro-1(2H)-pyrazinecarboxylate (171 mg, 0.440 mmol) and Cs2CO3 (299 mg, 0.918 mmol) in DME (5 mL) and water (1.67 mL) and PdCl2dppf (26.9 mg, 0.037 mmol) afforded the product (197 mg, 100%) as a brown solid. LCMS: [M+H]+=536.67.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(8-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate (196 mg, 0.366 mmol) in CH2Cl2 (5.0 mL) was added TFA (0.84 mL, 11.0 mmol). The reaction was stirred at RT for 96 h, then concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% ACN/H2O) to afford the TFA salt of the product (137.1 mg, 68%) as a yellow solid. LCMS: [M+H]+=436.51.
Step 3: 6-(2-amino-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.045 mmol), N,N-diisopropylethylamine (0.079 mL, 0.455 mmol) in DMF (1.0 mL), and (bromomethyl)cyclopropane (30.7 mg, 0.227 mmol) afforded the title compound (14.6 mg, 66%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.88 (br s, 1H), 7.52 (d, J=10.2 Hz, 1H), 7.30 (br d, J=8.1 Hz, 2H), 7.19 (s, 1H), 7.15 (br d, J=12.2 Hz, 1H), 6.87 (d, J=8.8 Hz, 2H), 6.18 (s, 2H), 3.03-3.11 (m, 4H), 2.83 (br t, J=6.2 Hz, 2H), 2.44-2.51 (m, 4H), 2.12 (d, J=6.6 Hz, 2H), 0.71-0.80 (m, 1H), 0.33-0.42 (m, 2H), 0.00 (q, J=4.7 Hz, 2H); LCMS: [M+H]+=490.60.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol) and N,N-diisopropylethylamine (0.079 mL, 0.456 mmol) in DMF (1.0 ml) and bromomethyl)cyclopropane (30.8 mg, 0.228 mmol) afforded the title compound (6.28 mg, 28%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.86 (br s, 1H), 7.54 (d, J=10.0 Hz, 1H), 7.36 (br d, J=7.6 Hz, 2H), 7.08-7.22 (m, 4H), 6.27 (s, 2H), br s, 2H), 2.80 (br t, J=6.1 Hz, 2H), 2.21 (br d, J=17.6 Hz, 2H), 1.88-2.07 (m, 1H), 1.54-1.72 (m, 4H), 0.76 (br s, 1H), 0.37 (br d, J=7.0 Hz, 2H), 0.00 (br s, 2H); LCMS: [M+H]+=489.66.
A procedure analogous to Example 177, Step 1 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol), 4-(morpholino)phenylboronic acid (24.55 mg, 0.119 mmol) Cs2CO3 (80 mg, 0.247 mmol) DME (2 mL), water (0.67 mL) and PdCl2dppf (7.23 mg, 9.88 μmol) afforded the title compound (13.7 mg, 32%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.00 (br s, 1H), 7.64 (br d, J=9.9 Hz, 1H), 7.44 (br d, J=8.1 Hz, 2H), 7.30 (s, 1H), 7.27 (br d, J=12.1 Hz, 1H), 6.99 (br d, J=8.4 Hz, 2H), 6.31 (br s, 2H), 3.75 (br s, 4H), 3.14 (br s, 4H), 2.94 (br d, J=5.5 Hz, 2H); LCMS: [M+H]+=437.52.
Step 1: 6-bromo-3-((dimethylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one
A mixture of 6-bromo-3-((methylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one (150 mg, 0.557 mmol), formaldehyde solution, 37% wt in water (0.124 mL, 1.67 mmol) was stirred overnight at RT with NaBH(OAc)3 (472 mg, 2.23 mmol) and glacial AcOH (0.016 mL, 0.279 mmol). The reaction mixture was partitioned between CH2Cl2 (5 mL) and 1N NaOH (aq) solution (7 mL). The layers were separated and the aqueous layer was further extracted with CH2Cl2 (5 mL). The combined organic extract was washed successively with water (5 mL), brine (5 mL), dried over Na2SO4 and concentrated to yield the title compound as an off white solid (154 mg, 98%). LCMS: [M+H]+=283.31.
Step 2: 3-((dimethylamino)methyl)-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 108, Step 2 using 6-bromo-3-((dimethylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one (151 mg, 0.533 mmol), bis(pinacolato)diboron (153 mg, 0.603 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (39.0 mg, 0.053 mmol) and KOAc (157 mg, 1.60 mmol) in 1,4-dioxane (10 mL) at 100° C. for 1 h afforded the crude product which was filtered through a thin pad of Celite, the filter cake was washed in with EtOAc and combined filtrate was concentrated to collect the crude product as a brown (176 mg, quantitative yield assuming 100% conversion). This was taken to the next step without any purification. LCMS: [M+H]+=331.64.
Step 3: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3-((dimethylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to that of Example 52, Step 1, by heating under microwave reaction conditions at 80° C. for 1 h, 5-bromo-6-fluoro-3-iodopyridin-2-amine (199 mg, 0.627 mmol), 3-((dimethylamino)methyl)-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (176 mg, 0.533 mmol), Na2CO3 (163 mg, 1.32 mmol), bis(triphenylphosphine)palladium(II) dichloride in CH3CN (7 mL) and water (2.5 mL), afforded the crude product. The reaction mixture was concentrated onto Celite® and silica gel chromatography (eluting with CH2Cl2 containing 0-2.5% MeOH and 0-0.25% NH4OH) afforded the product as a beige solid (134.5 mg, 55% yield). LCMS: [M+H]+=393.45.
Step 4: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-3-((dimethylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 177, Step 1 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3-((dimethylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.076 mmol)), 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (35.3 mg, 0.107 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (8.37 mg, 0.011 mmol), Cs2CO3 (74.6 mg, 0.229 mmol), degassed DME (4 mL) and water (1 mL) afforded the title compound as a beige solid (11 mg, 27%). 1H NMR (500 MHz, DMSO-d6) δ 7.91 (d, J=7.8 Hz, 1H), 7.63-7.55 (m, 1H), 7.51-7.37 (m, 5H), 7.02-6.91 (m, 2H), 6.23-6.08 (m, 2H), 3.77-3.75 (m, 1H), 3.18-3.14 (m, 4H), 3.10-3.05 (m, 1H), 2.86-2.79 (m, 1H), 2.72-2.67 (m, 1H), 2.62-2.56 (m, 4H), 2.43-2.37 (m, 1H), 2.35-2.30 (m, 1H), 2.22-2.15 (m, 6H), 1.05-0.97 (m, 6H); LCMS: [M+H]+=517.68.
A procedure analogous to that of Example 177 Step 1 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3-((dimethylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.076 mmol), 4-(morpholino)phenylboronic acid (22.11 mg, 0.107 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (8.37 mg, 0.011 mmol), Cs2CO3 (74.6 mg, 0.229 mmol), DME (4 mL) and water (1 mL) afforded the crude product which was concentrated onto Celite®. Silica gel chromatography (eluting with DCM containing 0-2% MeOH and 0-0.2% NH4OH), followed by reverse phase chromatography (C18; 0-40% CH3CN/water) afforded the title compound as a beige solid (9.5 mg, 25%). 1H NMR (500 MHz, DMSO-d6) δ 7.93-7.86 (m, 1H), 7.62-7.57 (m, 1H), 7.50-7.41 (m, 5H), 7.02-6.97 (m, 2H), 6.18 (s, 2H), 3.77-3.71 (m, 5H), 3.15-3.11 (m, 4H), 3.09-3.03 (m, 1H), 2.84-2.77 (m, 1H), 2.43-2.36 (m, 1H), 2.34-2.28 (m, 1H), 2.18 (s, 6H); LCMS: [M+H]+=476.69.
A procedure analogous to that of Example 177, Step 1 with 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3-((dimethylamino)methyl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.076 mmol), 4-chlorophenylboronic acid, pinacol ester (25.5 mg, 0.107 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (8.37 mg, 0.011 mmol) and Cs2CO3 (74.6 mg, 0.229 mmol), DME (4 mL) and water (1 mL) was used. The reaction mixture was concentrated onto Celite®. Silica gel chromatography (eluting with 0-70% EtOAc/hexanes), followed by reverse phase chromatography (C18; 0-40% acetonitrile/water) afforded the title compound as a beige solid (14 mg, 41%). 1H NMR (500 MHz, DMSO-d6) δ 7.97-7.87 (m, 1H), 7.72-7.65 (m, 1H), 7.63-7.58 (m, 2H), 7.51-7.44 (m, 5H), 6.47-6.35 (m, 2H), 3.79-3.72 (m, 1H), 3.10-3.02 (m, 1H), 2.87-2.77 (m, 1H), 2.44-2.37 (m, 1H), 2.35-2.29 (m, 1H), 2.19 (s, 6H); LCMS: [M+H]+=425.61.
A procedure similar to Example 177, Step 1 using (2-methylpyridine-4-yl) boronic acid (26.4 mg, 0.193 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (62 mg, 0.175 mmol), 1,4-dioxane (3 mL), Cs2CO3 (171 mg, 0.525 mmol) in water (1 mL) and Pd(dppf)Cl2 (19.21 mg, 0.026 mmol) afforded the title compound (41.0 mg, 61%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.42 (d, J=5.3 Hz, 1H), 8.12 (br s, 1H), 7.82 (d, J=10.1 Hz, 1H), 7.61 (d, J=10.1 Hz, 1H), 7.47 (s, 1H), 7.43 (d, J=7.0 Hz, 1H), 7.38 (br d, J=5.1 Hz, 1H), 6.59 (br s, 2H), 3.41 (dt, J=2.7, 6.5 Hz, 2H), 2.92 (br t, J=6.4 Hz, 2H), 2.48 (s, 3H); LCMS: [M+H]+=367.21.
A procedure similar to Example 177, Step 1 using 2-(cyclopropylmethoxy)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine (52.1 mg, 0.189 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (61 mg, 0.172 mmol), 1,4-dioxane (3 ml), Cs2CO3 (168 mg, 0.517 mmol) in water (1 mL) and Pd(dppf)Cl2 (18.90 mg, 0.026 mmol) afforded the title compound (54.4 mg, 71%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (d, J=5.3 Hz, 2H), 7.82 (d, J=10.0 Hz, 1H), 7.61 (d, J=10.0 Hz, 1H), 7.44 (d, J=7.1 Hz, 1H), 7.17 (d, J=5.4 Hz, 1H), 6.98 (s, 1H), 6.59 (br s, 2H), 4.10 (d, J=7.1 Hz, 2H), 3.41 (dt, J=6.5, 2.8 Hz, 2H), 2.92 (br t, J=6.5 Hz, 2H), 1.29-1.19 (m, 1H), 0.58-0.50 (m, 2H), 0.32 (br dd, J=1.3, 4.8 Hz, 2H); LCMS: [M+H]+=423.11.
A procedure similar to Example 177, Step 1 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (63 mg, 0.178 mmol), 3-(cyclopropylmethoxy)phenylboronic acid (37.6 mg, 0.196 mmol), 1,4-dioxane (3 mL) Cs2CO3 (174 mg, 0.534 mmol) in water (1 mL) and [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (19.52 mg, 0.027 mmol) afforded the title compound (26.7 mg, 34%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.67 (d, J=10.1 Hz, 1H), 7.61 (d, J=10.1 Hz, 1H), 7.43 (d, J=7.1 Hz, 1H), 7.30 (t, J=7.9 Hz, 1H), 7.08 (br d, J=7.5 Hz, 1H), 7.04 (s, 1H), 6.86 (dd, J=8.1, 2.1 Hz, 1H), 6.32 (s, 2H), 3.84 (d, J=7.0 Hz, 2H), 3.45-3.37 (m, 2H), 2.92 (br t, J=6.5 Hz, 2H), 1.26-1.16 (m, 1H), 0.59-0.51 (m, 2H), 0.35-0.27 (m, 2H); LCMS: [M+H]+=422.44.
A procedure analogous to Example 177, Step 1 using 4-(cyclopropylmethoxy)phenylboronic acid (37.0 mg, 0.193 mmol), 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (62 mg, 0.175 mmol), 1,4-dioxane (3 mL) and Cs2CO3 (171 mg, 0.525 mmol) in water (1 mL) and Pd(dppf)Cl2 (19.21 mg, 0.026 mmol) afforded the title compound (16.0 mg, 21%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.60 (dd, J=6.8, 10.1 Hz, 2H), 7.47-7.39 (m, 3H), 6.96 (d, J=8.8 Hz, 2H), 6.23 (s, 2H), 3.83 (d, J=7.0 Hz, 2H), 3.44-3.37 (m, 2H), 2.96-2.88 (m, 2H), 1.26-1.17 (m, 1H), 0.60-0.53 (m, 2H), 0.35-0.29 (m, 2H); LCMS: [M+H]+=422.44.
A procedure analogous to Example 191 using 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24.8 mg, 0.061 mmol) in CH2Cl2 (1 mL) was added N,N-diisopropylethylamine (0.053 mL, 0.307 mmol) at RT followed by 2-cyclopropylacetyl chloride (8.72 mg, 0.074 mmol) afforded the title compound (19.3 mg, 62%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.52-7.43 (m, 4H), 6.90 (d, J=8.8 Hz, 2H), 6.26 (s, 2H), 5.10-5.01 (m, 1H), 4.62-4.51 (m, 1H), 4.36-4.26 (m, 1H), 4.12-4.04 (m, 1H), 3.82-3.75 (m, 1H), 3.40 (br d, J=2.4 Hz, 2H), 2.95 (s, 2H), 2.03 (d, J=6.8 Hz, 2H), 0.98-0.87 (m, 1H), 0.48-0.38 (m, 2H), 0.13-0.05 (m, 2H); LCMS: [M+H]+=487.52.
A procedure similar to Example 218 using 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (33.7 mg, 0.083 mmol) in CH2Cl2 (1 mL), oxetan-3-one (45 mg, 0.624 mmol) and 2 drops glacial AcOH (40 mg, 0.666 mmol) afforded the title compound (13.8 mg, 34%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.59 (d, J=10.3 Hz, 1H), 7.51-7.41 (m, 4H), 6.89 (d, J=8.8 Hz, 2H), 6.24 (s, 2H), 4.93-4.84 (m, 1H), 4.57 (s, 2H), 4.40-4.32 (m, 2H), 3.83-3.71 (m, 3H), 3.44-3.38 (m, 3H), 3.19-3.12 (m, 2H), 2.97-2.92; (m, 2H); LCMS: [M+H]+=461.49.
Step 1: tert-butyl (2S,6R)-4-(4-bromophenyl)-2,6-dimethylpiperazine-1-carboxylate
A procedure similar to Example 108 Step 1 using 1-bromo-4-iodobenzene (560 mg, 1.98 mmol), cis-1-Boc-2,6-dimethyl-piperazine (636 mg, 2.97 mmol), tris(dibenzylideneacetone)dipalladium (0) (54.4 mg, 0.059 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (103 mg, 0.178 mmol) and Cs2CO3 (1935 mg, 5.94 mmol) afforded the product (269 mg, 37%) as a yellow oil. LCMS: [M+H]+=369.39.
Step 2: tert-butyl (2S,6R)-4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-2,6-dimethylpiperazine-1-carboxylate
A procedure similar to Example 43, Step 5 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.302 mmol), tert-butyl (2S,6R)-4-(4-bromophenyl)-2,6-dimethylpiperazine-1-carboxylate (93 mg, 0.252 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.43 mg, 0.025 mmol), aqueous K3PO4 (0.387 mL of a 1.3 M solution, 0.504 mmol), DME (4 mL) and water (2 mL) afforded the product (80 mg, 58%)1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.58 (d, J=10.1 Hz, 1H), 7.48-7.39 (m, 4H), 6.99 (d, J=8.9 Hz, 2H), 6.18 (s, 2H), 4.15-4.08 (m, 2H), 3.54 (br d, J=12.2 Hz, 2H), 3.40 (td, J=6.5, 2.7 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.81 (dd, J=12.2, 4.2 Hz, 2H), 1.43 (s, 9H), 1.25 (d, J=6.8 Hz, 6H); LCMS: [M +H]+=546.58.
Step 3: 6-(2-amino-5-(4-((3R,5S)-3,5-dimethylpiperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
In a vial was placed tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-cis-2,6-dimethylpiperazine-1-carboxylate (80 mg, 0.147 mmol), CH2Cl2 (5 mL) and TFA (2 mL, 0.147 mmol). The flask was mixed by vortexing and then allowed to sit for 2 h, when LCMS indicated complete conversion. The reaction was concentrated and then purified by loading onto a column containing PoraPak RxnCX acidic PE resin, washing (MeOH) and then eluting off the compound with 3% NH4OH in MeOH to provide the title compound (54 mg, 82%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.93 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.57 (d, J=10.1 Hz, 1H), 7.48-7.44 (m, 1H), 7.44 (s, 1H), 7.38 (d, J=7.8 Hz, 2H), 6.95 (d, J=8.9 Hz, 2H), 6.15 (s, 2H), 3.56 (dd, J=11.4, 2.1 Hz, 2H), 3.42-3.38 (m, 3H), 2.95 (t, J=6.5 Hz, 2H), 2.87-2.79 (m, 2H), 2.12 (t, J=11.0 Hz, 2H), 1.02 (d, J=6.2 Hz, 6H); LCMS: [M+H]+=446.36.
Step 1: (1S,5R)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane
To a microwave vial charged with (1 S,5R)-1-(4-bromophenyl)-3-azabicyclo[3.1.0]hexane (130 mg, 0.546 mmol), bis(pinacolato)diboron (152 mg, 0.601 mmol) and KOAc (161 mg, 1.638 mmol) was added anhydrous 1,4-dioxane (6.0 mL). The system was degassed then PdCl2dppf (39.9 mg, 0.055 mmol) was added. The mixture was flushed with nitrogen then heated at 100° C. 3 h. The reaction was diluted with acetonitrile, filtered through a pad of Celite, concentrated in vacuo and used as-is the next step assuming full conversion. LCMS: [M+H]+=286.45.
Step 2: 6-(5-(4-((1S,5R)-3-azabicyclo[3.1.0]hexan-1-yl)phenyl)-2-amino-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (130 mg, 0.367 mmol), (1 S,5R)-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (136 mg, 0.477 mmol) and Cs2CO3 (299 mg, 0.918 mmol), DME (5 mL), water (1.67 mL) and PdCl2dppf (26.9 mg, 0.037 mmol) afforded the title compound (58 mg, 37%) as a brown solid. LCMS: [M+H]+=433.57.
A procedure similar to Example 186 using 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (27.1 mg, 0.067 mmol) in DMF (1 mL), N,N-diisopropylethylamine (0.070 mL, 0.402 mmol) and (bromomethyl)cyclopropane (18.09 mg, 0.134 mmol) afforded the title compound (13.4 mg, 41%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 4H), 6.88 (d, J=8.8 Hz, 2H), 6.24 (s, 2H), 4.87-4.76 (m, 1H), 3.86-3.74 (m, 2H), 3.43-3.37 (m, 2H), 3.08-2.98 (m, 2H), 2.97-2.91 (m, 2H), 2.37-2.29 (m, 2H), 0.79-0.70 (m, 1H), 0.43-0.34 (m, 2H), 0.14-0.05 (m, 2H); LCMS: [M+H]+=459.53.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24.7 mg, 0.057 mmol) in DMF (1 mL), N,N-diisopropylethylamine (0.060 mL, 0.343 mmol) and (bromomethyl)cyclopropane (15.42 mg, 0.114 mmol) afforded the title compound (14.5 mg, 50%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.59 (d, J=10.1 Hz, 1H), 7.49-7.42 (m, 4H), 6.99 (d, J=8.7 Hz, 2H), 6.22 (s, 2H), 4.44-4.34 (m, 1H), 3.45-3.38 (m, 2H), 2.95 (br t, J=6.5 Hz, 2H), 2.84-2.74 (m, 2H), 2.31-2.22 (m, 2H), 2.19 (br d, J=6.2 Hz, 2H), 2.01-1.90 (m, 2H), 1.70-1.57 (m, 2H), 0.89-0.79 (m, 1H), 0.50-0.42 (m, 2H), 0.07 (q, J=4.9 Hz, 2H); LCMS: [M+H]+=487.60.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate
A procedure similar to Example 177, Step 1 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (842 mg, 2.378 mmol), tert-butyl 4-[4-(tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy]piperidine-1-carboxylate (1055 mg, 2.62 mmol), 1,4-dioxane (8 mL), Cs2CO3 (2.32 g, 7.13 mmol) in water (2 mL) and Pd(dppf)Cl2 (262 mg, 0.357 mmol) afforded the title compound (1.313 g, 95%) as a beige powder. LCMS: [M+H]+=551.55.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)piperidine-1-carboxylate (1.3129 g, 2.384 mmol) and CH2Cl2 (4 mL) and TFA (2 mL, 26.1 mmol) afforded the title compound (1.094 g, 97%) as a beige powder which was used for the next step. LCMS: [M+H]+=451.48.
Step 3: 6-(2-amino-5-(4-((1-(2-cyclopropylacetyl)piperidin-4-yl)oxy)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure analogous to Example 191 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (24.0 mg, 0.053 mmol) in CH2Cl2 (1 mL) was added N,N-diisopropylethylamine (0.046 mL, 0.266 mmol) and 2-cyclopropylacetyl chloride (7.58 mg, 0.064 mmol) afforded the title compound (13.9 mg, 47%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.60 (dd, J=10.1, 2.8 Hz, 2H), 7.48-7.39 (m, 3H), 7.02 (d, J=8.8 Hz, 2H), 6.24 (s, 2H), 4.70-4.59 (m, 1H), 3.92-3.80 (m, 1H), 3.72-3.64 (m, 1H), 3.43-3.37 (m, 2H), 3.28-3.20 (m, 1H), 2.96-2.87 (m, 2H), 2.27 (d, J=6.6 Hz, 2H), 2.00-1.85 (m, 2H), 1.64-1.55 (m, 1H), 1.54-1.45 (m, 1H), 1.01-0.89 (m, 1H), 0.49-0.40 (m, 2H), 0.15-0.08 (m, 2H); LCMS: [M+H]+=533.49.
A procedure similar to Example 186 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (24.5 mg, 0.054 mmol) and (bromomethyl)cyclopropane (14.68 mg, 0.109 mmol) afforded the title compound (13.5 mg, 47%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (br s, 1H), 7.60 (dd, J=10.1, 7.5 Hz, 2H), 7.42 (d, J=7.5 Hz, 3H), 6.98 (d, J=8.7 Hz, 2H), 6.23 (s, 2H), 4.42-4.34 (m, 1H), 3.43-3.37 (m, 2H), 2.95-2.88 (m, 2H), 2.85-2.73 (m, 2H), 2.31-2.23 (m, 2H), 2.19 (br d, J=6.4 Hz, 2H), 2.00-1.90 (m, 2H), 1.68-1.57 (m, 2H), 0.88-0.78 (m, 1H), 0.51-0.41 (m, 2H), 0.07 (q, J=5.1 Hz, 2H); LCMS: [M+H]+=505.28.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (26.1 mg, 0.058 mmol) in CH2Cl2 (1 ml) and oxetan-3-one (45 mg, 0.624 mmol) followed by 2 drops of glacial AcOH (40 mg, 0.666 mmol) afforded the title compound (10.0 mg, 32%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.60 (dd, J=10.1, 6.4 Hz, 2H), 7.46-7.40 (m, 3H), 6.99 (d, J=8.8 Hz, 2H), 6.23 (s, 2H), 4.53 (t, J=6.5 Hz, 2H), 4.42 (t, J=6.1 Hz, 3H), 3.40 (br s, 4H), 3.28-3.20 (m, 1H), 2.91 (s, 2H), 2.14-2.05 (m, 2H), 1.99-1.91 (m, 2H), 1.69-1.59 (m, 2H); LCMS: [M+H]+=507.38.
A procedure similar to Example 191 using 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (24.2 mg, 0.060 mmol) and N,N-diisopropylethylamine (0.052 mL, 0.299 mmol) in CH2Cl2 (3 mL) and acetic anhydride (5.66 μl, 0.060 mmol) afforded the title compound (19.5 mg, 69%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.52-7.43 (m, 4H), 6.90 (d, J=8.8 Hz, 2H), 6.26 (s, 2H), 5.08-5.00 (m, 1H), 4.56 (dd, J=8.6, 6.6 Hz, 1H), 4.29 (dd, J=6.5, 10.5 Hz, 1H), 4.09 (dd, J=9.2, 3.7 Hz, 1H), 3.77 (dd, J=10.5, 3.7 Hz, 1H), 3.44-3.37 (m, 2H), 2.95 (s, 2H), 1.79 (s, 3H); LCMS: [M+H]+=447.42.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (24.9 mg, 0.055 mmol), N,N-diisopropylethylamine (0.048 ml, 0.276 mmol) in CH2Cl2 (3 mL) and acetic anhydride (5.22 μl, 0.055 mmol) afforded the title compound (20.1 mg, 70%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.60 (dd, J=2.6, 10.1 Hz, 2H), 7.48-7.40 (m, 3H), 7.02 (d, J=8.9 Hz, 2H), 6.24 (s, 2H), 4.68-4.60 (m, 1H), 3.88-3.79 (m, 1H), 3.70-3.62 (m, 1H), 3.43-3.38 (m, 2H), 3.37-3.34 (m, 1H), 3.28-3.20 (m, 1H), 2.96-2.88 (m, 2H), 2.01 (s, 3H), 1.99-1.93 (m, 1H), 1.93-1.85 (m, 1H), 1.66-1.57 (m, 1H), 1.55-1.45 (m, 1H); LCMS: [M+H]+=493.46.
To a solution of 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.050 mmol) and N,N-diisopropylethylamine (0.087 ml, 0.498 mmol) in DMF (1.0 mL) was added 2-iodopropane (42.3 mg, 0.249 mmol). The reaction was stirred at RT for 72 h, concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH, followed by reverse phase chromatography (C18, 0-100% ACN/H2O) to afford the title compound (8.9 mg, 42%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br. s., 1H), 7.84 (d, J=8.0 Hz, 1H), 7.54 (d, J=10.0 Hz, 1H), 7.40 (d, J=Hz, 1H), 7.37 (s, 1H), 7.18-7.24 (m, 2H), 7.03 (d, J=8.0 Hz, 1H), 6.19 (s, 2H), 3.57 (s, 2H), 3.34 (td, J=6.5, 2.6 Hz, 2H), 2.89 (t, J=6.5 Hz, 2H), 2.76-2.82 (m, 1H), 2.71-2.76 (m, 2H), 2.60-2.65 (m, 2H), 0.99 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=431.56.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(3-(piperazin-1-ylmethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol, preparation described in Example 265 Step 2) and N,N-diisopropylethylamine (0.080 mL, 0.458 mmol) in DMf (1.0 mL) was added 2-bromoethyl methyl ether (31.8 mg, 0.229 mmol). The reaction was stirred at RT overnight. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH, followed by reverse phase chromatography (C18, 0-100% ACN/H2O) to afford the title compound (10.3 mg, 46%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br. s., 1H), 7.91 (d, J=8.0 Hz, 1H), 7.64 (d, J=10.0 Hz, 1H), 7.41-7.51 (m, 4H), 7.37 (t, J=7.5 Hz, 1H), 7.24 (d, J=7.5 Hz, 1H), 6.31 (s, 2H), 3.48 (s, 2H), 3.38-3.44 (m, 4H), 3.21 (s, 3H), 2.96 (t, J=6.4 Hz, 2H), 2.44 (t, J=5.9 Hz, 2H), 2.39 (br. s., 5H); LCMS: [M+H]+=490.67.
A procedure similar to Example 306, Step 3 using 6-(2-amino-6-fluoro-5-(3-(piperazin-1-ylmethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol) and N,N-diisopropylethylamine (0.080 mL, 0.458 mmol) in DMF (1.0 mL) and 2-iodopropane (39.0 mg, 0.229 mmol) afforded the title compound (3.2 mg, 15%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br. s., 1H), 7.84 (d, J=7.8 Hz, 1H), 7.56 (d, J=10.2 Hz, 1H), 7.33-7.43 (m, 4H), 7.29 (t, J=7.6 Hz, 1H), 7.16 (d, J=7.5 Hz, 1H), 6.24 (s, 2H), 3.40 (s, 2H), 3.34 (td, J=6.4, 2.6 Hz, 2H), 2.88 (t, J=6.4 Hz, 2H), 2.48-2.54 (m, 1H), 2.25-2.39 (m, 7H), 0.87 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=474.50.
A procedure analogous to Example 246 with 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 3-(4-methylpiperazine-1-carbonyl)phenylboronic acid, pinacol ester (58.9 mg, 0.178 mmol), Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (43.4 mg, 64%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br. s., 1H), 7.95 (br. s., 1H), 7.92 (d, J=8.0 Hz, 1H), 7.69 (d, J=10.2 Hz, 1H), 7.55 (s, 1H), 7.45-7.52 (m, 3H), 7.31 (d, J=7.6 Hz, 1H), 6.39 (s, 2H), 3.62 (br. s., 2H), 3.41 (td, J=6.5, 2.6 Hz, 2H), 2.96 (t, J=6.5 Hz, 2H), 2.49-2.54 (m, 1H), 2.23-2.43 (m, 4 H), 2.19 (s, 3H); LCMS: [M+H]+=460.58.
A procedure similar to Example 246 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (134 mg, 0.350 mmol), 5-bromo-2-(4-methylpiperazin-1-yl)aniline dihydrochloride (100 mg, 0.291 mmol), Cs2CO3 (237 mg, 0.729 mmol), DME (12 mL), water (4 mL) and PdCl2dppf (21.33 mg, 0.029 mmol) the title compound (43.7 mg, 34%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br. s., 1H), 7.91 (d, J=8.0 Hz, 1H), 7.53 (d, J=10.0 Hz, 1H), 7.44-7.48 (m, 1H), 7.43 (s, 1H), 6.92 (d, J=8.2 Hz, 1H), 6.88 (s, 1H), 6.74 (d, J=8.2 Hz, 1H), 6.19 (s, 2H), 4.74 (s, 2H), 3.41 (td, J=6.5, 2.6 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H), 2.82 (br. s., 4H), 2.50-2.51 (m, 4H), 2.24 (s, 3H); LCMS: [M+H]+=447.61.
A procedure analogous to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.149 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (48.8 mg, 0.178 mmol), Cs2CO3 (121 mg, 0.372 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (10.88 mg, 0.015 mmol) afforded the title compound (14.7 mg, 25%) as beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.88 (br. s., 1H), 7.82-7.86 (m, 2H), 7.79 (d, J=8.1 Hz, 1H), 7.65 (d, J=10.2 Hz, 1H), 7.47 (d, J=8.2 Hz, 1H), 7.45 (s, 1H), 7.41 (d, J=8.0 Hz, 1H), 7.39 (s, 1H), 6.36 (s, 2H), 3.30-3.37 (m, 4H), 2.84-2.92 (m, 4H); LCMS: [M+H]+=403.48.
Step 1: tert-butyl 7-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinoline-2(1H)-carboxylate
To a suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (110 mg, 0.327 mmol), tert-butyl 7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinoline-2(1H)-carboxylate (141 mg, 0.393 mmol) and Cs2CO3 (267 mg, 0.818 mmol) in DME (4 mL) and water (1.3 mL) was added PdCl2dppf (23.94 mg, 0.033 mmol). The mixture was flushed with nitrogen and heated in the microwave at 90° C. for 2 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH to afford the product (141 mg, 88%) as a beige solid. LCMS: [M+H]+=489.48.
Step 2: 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-7-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 7-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-3,4-dihydroisoquinoline-2(1H)-carboxylate (141 mg, 0.289 mmol) in CH2Cl2 (5.0 mL) was added TFA (0.66 mL, 8.66 mmol). The reaction was stirred at RT overnight. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the trifluoroacetate salt of the title compound (132 mg, 91%) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 8.95 (br. s., 2H), 7.96 (br. s., 1H), 7.92 (d, J=7.8 Hz, 1H), 7.64 (d, J=10.2 Hz, 1H), 7.45-7.50 (m, 2H), 7.44 (s, 2H), 7.29 (d, J=8.2 Hz, 1H), 6.36 (s, 2H), 4.31 (s, 2H), 3.42 (t, J=6.4 Hz, 4H), 3.01 (t, J=6.2 Hz, 2H), 2.96 (t, J=6.5 Hz, 2H); LCMS: [M+H]+=389.50.
A procedure similar to Example 306, Step 3 using 6-(2-amino-6-fluoro-5-(1,2,3,4-tetrahydroisoquinolin-7-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one-TFA (25 mg, 0.050 mmol), N,N-diisopropylethylamine (0.087 mL, 0.498 mmol), DMF (1.0 mL) and 2-iodopropane (42.3 mg, 0.249 mmol) afforded the title compound (4.8 mg, 2%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.87 (br. s., 1H), 7.84 (d, J=8.0 Hz, 1H), 7.55 (d, J=10.2 Hz, 1H), 7.39 (d, J=8.1 Hz, 1H), 7.37 (s, 1H), 7.22 (d, J=8.0 Hz, 1H), 7.18 (s, 1H), 7.05 (d, J=8.0 Hz, 1H), 6.19 (s, 2H), 3.59 (s, 2H), 3.34 (td, J=6.42, 2.6 Hz, 2H), 2.88 (t, J=6.5 Hz, 2H), 2.78 (dt, J=13.1, 6.5 Hz, 1H), 2.69-2.74 (m, 2H), 2.60-2.65 (m, 2H), 0.99 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=431.56.
A procedure analogous to Example 246 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (41 mg, 0.116 mmol), 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (45.9 mg, 0.139 mmol) Cs2CO3 (94 mg, 0.289 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (8.47 mg, 0.012 mmol) afforded the title compound (34.7 mg, 63%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99 (br. s., 1H), 7.62 (d, J=10.2 Hz, 1H), 7.41 (d, J=8.0 Hz, 2H), 7.26 (d, J=12.0 Hz, 1H) 7.30 (s, 1H), 6.97 (d, J=8.8 Hz, 2H), 6.29 (s, 2H), 3.12-3.19 (m, 4H), 2.94 (t, J=6.2 Hz, 2H), 2.68 (dt, J=13.0, 6.5 Hz, 1H), 2.55-2.61 (m, 4H), 1.01 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=478.51.
A procedure similar to Example 191 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (28.1 mg, 0.065 mmol) in CH2Cl2 (1 mL), N,N-diisopropylethylamine (0.057 ml, 0.325 mmol) and 2-cyclopropylacetyl chloride (9.24 mg, 0.078 mmol) afforded (20.1 mg, 57%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.60 (d, J=10.1 Hz, 1H), 7.50-7.43 (m, 4H), 7.03 (d, J=8.8 Hz, 2H), 6.23 (s, 2H), 4.68-4.61 (m, 1H), 3.92-3.82 (m, 1H), 3.73-3.64 (m, 1H), 3.40 (dt, J=2.6, 6.4 Hz, 2H), 3.29-3.21 (m, 1H), 2.95 (br t, J=6.5 Hz, 2H), 2.27 (d, J=6.6 Hz, 2H), 2.01-1.86 (m, 2H), 1.65-1.56 (m, 1H), 1.55-1.45 (m, 1H), 0.99-0.91 (m, 1H), 0.50-0.40 (m, 2H), 0.16-0.08 (m, 2H); LCMS: [M+H]+=515.51.
A procedure similar to Example 218 using 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (25.4 mg, 0.059 mmol), oxetan-3-one (45 mg, 0.624 mmol) in CH2Cl2 (1 mL), 2 drops of glacial AcOH (40 mg, 0.666 mmol) and NaBH(OAc)3 (137 mg, 0.646 mmol) afforded the title compound (3.3 mg, 11%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (br d, J=7.5 Hz, 1H), 7.59 (br d, J=9.7 Hz, 1H), 7.51-7.38 (m, 3H), 6.99 (br d, J=7.0 Hz, 2H), 6.23 (br s, 2H), 5.76 (br s, 1H), 4.75-4.67 (m, 1H), 4.67-4.60 (m, 1H), 4.57-4.50 (m, 2H), 4.48-4.37 (m, 2H), 3.45-3.32 (m, 4H), 3.01-2.88 (m, 2H), 2.15-2.05 (m, 2H), 2.01-1.88 (m, 2H), 1.71-1.57 (m, 2H); LCMS: [M+H]+=489.48.
Step 1: tert-butyl 3-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)azetidine-1-carboxylate
In a vial were added 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (781 mg, 2.205 mmol), tert-butyl 3-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)azetidine-1-carboxylate (910.3 mg, 2.43 mmol) and 1,4-dioxane (8 ml). Then a solution of Cs2CO3 (2155 mg, 6.62 mmol) in Water (2 mL) was added at RT and the reaction mixture was degassed under argon for 15 minutes. Then [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (242 mg, 0.331 mmol) was added and the reaction mixture was heated at 90° C. for overnight. The reaction mixture was dry loaded onto Celite® and purified by flash chromatography (0-10% DCM/MeOH) to afford the product (1.152 g, 100%) as a beige powder. [M+H]+ 523.48.
Step 2: 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 with tert-butyl 3-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenoxy)azetidine-1-carboxylate (1152 mg, 2.21 mmol) and TFA (4 mL, 52.2 mmol) in CH2Cl2 (10 mL) afforded the title compound TFA salt (897.7 mg, 72%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.90 (br s, 2H from TFA), 8.11 (br s, 1H), 7.61 (dd, J=10.1, 3.3 Hz, 2H), 7.49 (d, J=8.1 Hz, 2H), 7.42 (d, J=7.0 Hz, 1H), 6.91 (d, J=8.8 Hz, 2H), 6.28 (s, 2H), 5.14-5.06 (m, 1H), 4.50-4.42 (m, 2H), 4.03-3.98 (m, 2H), 3.43-3.38 (m, 2H), 2.95-2.88 (m, 2H); LCMS: [M+H]+=423.34.
Step 1: tert-butyl 3-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-3-fluoroazetidine-1-carboxylate
A procedure similar to Example 43, Step 5 using tert-butyl 3-(4-bromophenyl)-3-fluoroazetidine-1-carboxylate (516 mg, 1.56 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (719 mg, 1.88 mmol), 1,4-dioxane (10 mL) and K3PO4 (995 mg, 4.69 mmol) in water (2 mL) and XPhos Pd G2 (123 mg, 0.156 mmol) afforded the product (792 mg, 100%) as a beige powder. LCMS: [M+H]+=507.53.
Step 2: 6-(2-amino-6-fluoro-5-(4-(3-fluoroazetidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 144, Step 3 using tert-butyl 3-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)-3-fluoroazetidine-1-carboxylate (0.792 g, 1.56 mmol), TFA (4 mL, 52.2 mmol) and CH2Cl2 (10 mL) afforded the title compound (700.6 mg, 82%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 9.26 (br s, 2H from TFA), 7.96 (br s, 1H), 7.92 (d, J=7.8 Hz, 1H), 7.73-7.67 (m, 3H), 7.61 (d, J=8.2 Hz, 2H), 7.47 (d, J=8.1 Hz, 1H), 7.45 (s, 1H), 6.42 (s, 2H), 4.67-4.57 (m, 2H), 4.55-4.45 (m, 2H), 3.41 (dt, J=6.4, 2.6 Hz, 2H), 2.95 (t, J=6.5 Hz, 2H); LCMS: [M+H]+=407.39.
A procedure similar to Example 191 using 6-(2-amino-5-(4-(azetidin-3-yloxy)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetic acid (23.4 mg, 0.044 mmol) and N,N-diisopropylethylamine (0.038 mL, 0.218 mmol) in CH2Cl2 (3 mL) and acetic anhydride (4.12 μl, 0.044 mmol) afforded the title compound (15.6 mg, 73%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.61 (d, J=10.1 Hz, 2H), 7.47 (br d, J=8.1 Hz, 2H), 7.43 (d, J=7.0 Hz, 1H), 6.89 (d, J=8.7 Hz, 2H), 6.27 (s, 2H), 5.09-5.01 (m, 1H), 4.55 (dd, J=8.9, 6.8 Hz, 1H), 4.28 (dd, J=10.3, 6.5 Hz, 1H), 4.09 (dd, J=9.3, 3.4 Hz, 1H), 3.77 (dd, J=10.5, 3.6 Hz, 1H), 3.43-3.38 (m, 2H), 2.92 (br t, J=6.4 Hz, 2H), 1.79 (s, 3H); LCMS: [M+H]+=465.40.
A procedure similar to Example 191 with 6-(2-amino-6-fluoro-5-(4-(3-fluoroazetidin-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetic acid (24.3 mg, 0.047 mmol), N,N-diisopropylethylamine (0.041 ml, 0.233 mmol) in CH2Cl2 (3 mL) and acetic anhydride (4.41 μl, 0.047 mmol) afforded the title compound (15.5 mg, 70%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 7.92 (br dd, J=7.6, 12.0 Hz, 2H), 7.76-7.38 (m, 7H), 6.38 (br s, 2H), 4.72-4.52 (m, 2H), 4.39-4.20 (m, 2H), 3.45-3.38 (m, 2H), 3.02-2.89 (m, 2H), 1.87 (br s, 3H); LCMS: [M+H]+=449.37.
A procedure analogous to Example 246 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.100 mmol), (1R,5S)-1-(4-bromophenyl)-3-methyl-3-azabicyclo[3.1.0]hexane (30.2 mg, 0.120 mmol, preparation described in Example 14), Cs2CO3 (81 mg, 0.249 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (7.29 mg, 9.97 μmol) afforded the title compound (14.4 mg, 32%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.61 (dd, J=10.0, 5.0 Hz, 2H), 7.48-7.40 (m, 3H), 7.17 (br d, J=8.1 Hz, 2H), 6.29 (br s, 2H), 3.40 (br d, J=4.2 Hz, 2H), 3.01 (br s, 1H), 2.92 (br t, J=6.2 Hz, 2H), 2.47-2.38 (m, 1H), 2.32 (br s, 3H), 1.81 (br s, 1H), 1.35 (br s, 1H), 0.78 (br s, 1H); LCMS: [M+H]+=447.42.
A procedure analogous to Example 246 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.100 mmol), (1 S,5R)-1-(4-bromophenyl)-3-methyl-3-azabicyclo[3.1.0]hexane (30.2 mg, 0.120 mmol, preparation described in Example 106), Cs2CO3 (81 mg, 0.249 mmol), DME (4 mL), water (1.3 mL) and PdCl2dppf (7.29 mg, 9.97 μmol) afforded the title compound (13.7 mg, 31%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.11 (br s, 1H), 7.62 (dd, J=5.4, 10.0 Hz, 2H), 7.49-7.41 (m, 3H), 7.19 (br d, J=7.9 Hz, 2H), 6.30 (br s, 2H), 3.40 (br d, J=4.0 Hz, 2H), 3.03 (br s, 1H), 2.92 (br t, J=6.2 Hz, 2H), 2.42-2.25 (m, 3H), 1.83 (br s, 1H), 1.36 (br t, J=3.9 Hz, 1H), 0.80 (br s, 1H); LCMS: [M+H]+=447.48.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2,3-difluorophenyl)piperazine-1-carboxylate
To a degassed suspension of 1,4-dibromo-2,3-difluorobenzene (0.5 g, 1.8 mmol), tert-butyl piperazine-1-carboxylate (0.37 g, 2.0 mmol), BINAP (0.079 g, 0.1 mmol), sodium tert-butoxide (0.21 g, 2.2 mmol) in toluene (10 mL) was added Pd2(dba)3 (0.08 g, 0.1 mmol) at RT under argon atmosphere. The reaction mixture was heated to 85° C. for 4 h before cooling to RT. The mixture was filtered through a Celite® bed, which was washed with EtOAc (300 mL). The combined filtrate was concentrated under reduced pressure to give a residue which was purified by column chromatography (silica gel, 100-200 mesh) eluting with 0-20% EtOAc in pet ether to afford the product (0.4 g, 58%) as an off white solid. LCMS: [M +H]+=377.31.
Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2,3-difluorophenyl)piperazine-1-carboxylate
A procedure analogous to Example 246 using 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (98 mg, 0.254 mmol), tert-butyl 4-(4-bromo-2,3-difluorophenyl)piperazine-1-carboxylate (80 mg, 0.212 mmol), Cs2CO3 (173 mg, 0.530 mmol), DME (12 mL), water (4 mL) and PdCl2dppf (15.52 mg, 0.021 mmol) afforded the product (30.4 mg, 26%) as a beige solid. LCMS: [M+H]+=554.66.
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2,3-difluorophenyl)piperazine-1-carboxylate (30 mg, 0.054 mmol) in CH2Cl2 (5.0 mL) was added TFA (0.12 mL, 1.63 mmol). The reaction was stirred at RT for 16 h and concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the TFA salt of the title compound (17 mg, 55%) as an off-white solid. LCMS: [M+H]+=454.50.
Step 3: 6-(2-amino-5-(2,3-difluoro-4-(4-isopropylpiperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 306, Step 3 using 6-(2-amino-5-(2,3-difluoro-4-(piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (17 mg, 0.030 mmol), N,N-diisopropylethylamine (0.052 mL, 0.300 mmol) in DMF (1.0 mL) and 2-iodopropane (25.5 mg, 0.150 mmol) afforded the title compound (2.4 mg, 16%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.90 (d, J=7.9 Hz, 1H), 7.52 (d, J=9.5 Hz, 1H), 7.47-7.41 (m, 2H), 7.18 (br t, J=7.9 Hz, 1H), 6.88 (br t, J=8.1 Hz, 1H), 6.41 (s, 2H), 3.41-3.38 (m, 2H), 3.14-3.01 (m, 4H), 2.94 (br t, J=6.5 Hz, 2H), 2.68 (br d, J=3.5 Hz, 1H), 2.66-2.56 (m, 4H), 1.01 (br d, J=4.2 Hz, 6H); LCMS: [M+H]+=496.43.
A procedure analogous to that of Example 177, Step 1 using 4-(4-bromophenyl)thiomorpholine-1,1-dione (50 mg, 0.172 mmol) with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (97 mg, 0.241 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (17.65 mg, 0.024 mmol), Cs2CO3 (168 mg, 0.517 mmol), DME (3.5 mL) and water (1 mL) afforded the title compound as a beige solid (14 mg, 16%). 1H NMR (500 MHz, DMSO-d6) δ 8.18-8.06 (m, 1H), 7.67-7.58 (m, 2H), 7.51-7.39 (m, 3H), 7.22-6.99 (m, 2H), 6.32-6.13 (m, 2H), 3.87-3.78 (m, 4H), 3.43-3.41 (m, 2H), 3.13 (br s, 4H), 2.99-2.87 (m, 2H); LCMS: [M+H]+=485.42.
A procedure analogous to Example 177, Step 1, with 4-(4-bromophenyl)thiomorpholine (60 mg, 0.232 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (131 mg, 0.325 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (23.81 mg, 0.033 mmol), Cs2CO3 (227 mg, 0.697 mmol), DME (3.5 mL) and water (1 mL) afforded the title compound as a beige solid (39 mg, 35%). 1H NMR (500 MHz, DMSO-d6) 5=8.19-8.07 (m, 1H), 7.65-7.56 (m, 2H), 7.47-7.34 (m, 3H), 7.02-6.90 (m, 2H), 6.25-6.11 (m, 2H), 3.65-3.52 (m, 4H), 3.44-3.40 (m, 2H), 2.95-2.89 (m, 2H), 2.73-2.60 (m, 4H); LCMS: [M+H]+=453.33.
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yloxy)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (45.7 mg, 0.106 mmol) in DMF (2 mL) was added Et3N (0.015 ml, 0.106 mmol) at RT followed by Biotin-Osu (36.1 mg, 0.106 mmol). The reaction mixture was stirred at 24° C. for 3 h then dry loaded onto Celite® and purified by flash chromatography (0-20% DCM/MeOH) to afford the title compound (59.9 mg, 82%) as a white powder. 1H NMR (500 MHz, DMSO-d6) δ 9.37 (br s, 1H), 7.94 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.60 (d, J=10.3 Hz, 1H), 7.50-7.42 (m, 4H), 7.03 (d, J=8.7 Hz, 2H), 6.43 (s, 1H), 6.35 (s, 1H), 6.23 (s, 2H), 5.75 (s, 1H), 4.69-4.60 (m, 1H), 4.35-4.27 (m, 1H), 4.17-4.11 (m, 1H), 3.91-3.81 (m, 1H), 3.75-3.66 (m, 1H), 3.43-3.37 (m, 2H), 3.28-3.21 (m, 1H), 3.14-3.00 (m, 3H), 2.95 (br t, J=6.4 Hz, 2H), 2.82 (dd, J=5.1, 12.4 Hz, 1H), 2.61-2.55 (m, 1H), 2.33 (br t, J=7.4 Hz, 2H), 2.03-1.94 (m, 1H), 1.93-1.85 (m, 1H), 1.68-1.57 (m, 2H), 1.51 (br d, J=7.7 Hz, 4H), 1.40-1.30 (m, 2H), 1.25-1.12 (m, 4H); LCMS: [M+H]+=659.58.
Step 1: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-chloro-3,4-dihydroisoquinolin-1(2H)-one
To a degassed solution of 5-bromo-6-fluoro-3-iodopyridin-2-amine (309 mg, 0.976 mmol) and (7-chloro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)boronic acid (200 mg, 0.887 mmol) in acetonitrile (6 ml), in a microwave vial, was added a solution of sodium carbonate monohydrate (231 mg, 1.86 mmol) in water (1.5 mL). The system was degassed and bis(triphenylphosphine)palladium(II) dichloride (74.7 mg, 0.106 mmol) was added. The reaction was further flushed with nitrogen and heated in the microwave at 80° C. for 3 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-100% EtOAc/Hexanes to give the product (62.6 mg, 19%) as a beige solid. LCMS: [M+H]+=370.08.
Step 2: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-7-chloro-3,4-dihydroisoquinolin-1(2H)-one
To a suspension of 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-chloro-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.081 mmol), 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (32.1 mg, 0.097 mmol) and Cs2CO3 (65.9 mg, 0.202 mmol) in a 3:1 mixture of DML (4 mL): water (1.3 mL) was added PdCl2dppf (5.92 mg, 8.09 μmol). The mixture was flushed with nitrogen and heated in the microwave at 90° C. for 2 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH, followed by reverse phase chromatography (C18, 0-100% ACN/H2O). The desired fractions were then passed through an Isolute SCX-2 cation exchange resin cartridge eluting with 3% NH3 in MeOH to give the title compound (16.8 mg, 42%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.12 (br. s., 1H), 7.90 (s, 1H), 7.51 (d, J=10.3 Hz, 1H), 7.40 (s, 1H), 7.36 (d, J=8.2 Hz, 2H), 6.95 (d, J=8.7 Hz, 2H), 6.07 (s, 2H), 3.40 (d, J=2.1 Hz, 2H), 3.14 (br. s., 4H), 2.92 (t, J=6.4 Hz, 2H), 2.65 (d, J=19.6 Hz, 1H), 2.58 (br. s., 4H), 1.01 (d, J=6.11 Hz, 6H); LCMS: [M+H]+=494.38.
To a solution of 6-(2-amino-6-fluoro-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetate (25 mg, 0.046 mmol) and N,N-diisopropylethylamine (0.079 ml, 0.456 mmol) in DMF (1 mL) was added 2-bromoethyl methyl ether (31.7 mg, 0.228 mmol). The reaction was stirred at RT overnight. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH, followed by reverse phase chromatography (C18, 0-100% ACN/H2O). The desired fractions were then passed through an Isolute SCX-2 cation exchange resin cartridge eluting with 3% NH3 in MeOH to give the title compound (14.6 mg, 65%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99 (br. s., 1H), 7.67 (d, J=10.2 Hz, 1H), 7.47 (d, J=7.5 Hz, 2H), 7.23-7.33 (m, 4H), 6.39 (s, 2H), 3.45 (t, J=5.7 Hz, 2H), 3.24 (s, 3H), 2.99 (d, J=7.3 Hz, 2H), 2.93 (t, J=6.2 Hz, 2H), 2.1 (br. s., 2H), 1.71-1.79 (m, 2H), 1.61-1.70 (m, 2H); LCMS: [M+H]+=493.57.
A procedure analogous to Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol), (R)-2-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine (2.2 mL, 0.119 mmol, 17.5 mg/ml in ACN) and Cs2CO3 (80 mg, 0.247 mmol) in a 3:1 mixture of DME (2 mL): water (0.67 mL) and PdCl2dppf (7.23 mg, 9.88 μmol) afforded the title compound (8.9 mg, 19%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99 (br. s., 1H), 7.62 (d, J=10.2 Hz, 1H), 7.42 (d, J=8.0 Hz, 2H), 7.29 (s, 1H), 7.26 (d, J=12.0 Hz, 1H), 7.00 (d, J=8.8 Hz, 2H), 6.29 (s, 2H), 3.97 (dd, J=11.3, 2.2 Hz, 1H), 3.56-3.65 (m, 2H), 3.53 (d, J=11.9 Hz, 1H), 3.24 (ddd, J=10.1, 6.5, 2.2 Hz, 1H), 2.93 (t, J=6.2 Hz, 2H), 2.66 (td, J=11.8, 3.4 Hz, 1H), 2.44 (t, J=11.2 Hz, 1H), 1.73 (dq, J=13.4, 6.7 Hz, 1H), 0.96 (dd, J=6.7, 4.2 Hz, 6H); LCMS: [M+H]+=479.09.
A procedure analogous to Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol), (1R,5S)-3-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (35.5 mg, 0.119 mmol) and Cs2CO3 (80 mg, 0.247 mmol) in a 3:1 mixture of DME (2 mL): water (0.67 ml) and PdCl2dppf (7.23 mg, 9.88 μmol) afforded the title compound (16.4 mg, 37%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99 (br. s., 1H), 7.65 (d, J=10.2 Hz, 1H), 7.46 (d, J=7.5 Hz, 2H), 7.29 (s, 1H), 7.26 (d, J=12.1 Hz, 1H), 7.18 (d, J=8.3 Hz, 2H), 6.39 (s, 2H), 3.28 (d, J=8.2 Hz, 1H), 3.00 (d, J=7.8 Hz, 1H), 2.93 (t, J=6.2 Hz, 2H), 2.42 (br. s., 1H), 2.31 (br. s., 3H), 1.77-1.85 (m, 1H), 1.35 (t, J=4.0 Hz, 1H), 0.77 (d, J=3.91 Hz, 1H); LCMS: [M+H]+=447.32.
A procedure similar to Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol), (1 S,5R)-3-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (35.5 mg, 0.119 mmol) and Cs2CO3 (80 mg, 0.247 mmol) in a 3:1 mixture of DME (2 mL): water (0.67 mL) and PdCl2dppf (7.23 mg, 9.88 μmol) afforded the title compound (14.7 mg, 33%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99 (br. s., 1H), 7.65 (d, J=10.0 Hz, 1H), 7.46 (d, J=7.5 Hz, 2H), 7.29 (s, 1H), 7.26 (d, J=12.0 Hz, 1H), 7.17 (d, J=8.3 Hz, 2H), 6.39 (s, 2H), 3.26 (d, J=8.4 Hz, 1H), 2.98 (d, J=8.7 Hz, 1H), 2.93 (t, J=6.2 Hz, 2H), 2.39 (d, J=6.1 Hz, 1H), 2.30 (s, 3H), 1.79 (dt, J=7.7, 3.8 Hz, 1H), 1.35 (t, J=4.0 Hz, 1H), 0.76 (dd, J=7.9, 3.7 Hz, 1H); LCMS: [M+H]+=447.26.
To a stirred solution of 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (25 mg, 0.052 mmol) in 1,4-dioxane (2 ml) was added sodium methoxide, 0.5M in MeOH (0.16 mL, 0.079 mmol). The mixture was stirred at RT overnight. The reaction was quenched with saturated aqueous NH4Cl solution, then the volatiles were removed in vacuo. The material was diluted with water and the suspension was filtered. The crude material was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the title compound (14.9 mg, 58%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.69 (br. s., 1H), 7.61 (d, J=10.2 Hz, 1H), 7.39 (d, J=8.1 Hz, 2H), 7.04 (s, 1H), 6.98 (d, J=2.7 Hz, 2H), 6.96 (s, 1H), 6.20 (s, 2H), 3.81 (s, 3H), 3.23-3.28 (m, 2H), 3.10-3.17 (m, 4H), 2.84 (t, J=6.0 Hz, 2H), 2.67 (dt, J=13.0, 6.5 Hz, 1H), 2.54-2.60 (m, 4H), 1.00 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=490.43.
To a stirred solution of 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (25 mg, 0.052 mmol) in tetrahydrofuran (THF) (2.0 mL) was added K2CO3 (21.70 mg, 0.157 mmol) and dimethylamine, 2.0M in THF (0.79 mL, 1.57 mmol) solution. The mixture was stirred at 65° C. overnight. LCMS analysis showed ˜78% conversion of the starting material. The reaction was further heated at 80° C. overnight. The reaction was quenched with saturated aqueous NH4Cl solution, then the volatiles were removed in vacuo. The material was diluted with water and the suspension was filtered. The crude material was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to give the title compound (9.2 mg, 35%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.71 (br s, 1H), 7.57 (d, J=10.3 Hz, 1H), 7.39 (br d, J=8.1 Hz, 2H), 6.97 (br d, J=8.6 Hz, 2H), 6.84 (s, 1H), 6.75 (s, 1H), 6.11 (s, 2H), 3.27 (br d, J=4.5 Hz, 2H), 3.15 (br s, 4H), 2.86-2.77 (m, 8H), 2.59 (br s, 4H), 1.02 (br d, J=5.1 Hz, 6H); LCMS: [M+H]+=503.53.
To a stirred solution of 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (25 mg, 0.052 mmol) in 1,4-dioxane (2.0 mL) was added K2CO3 (21.70 mg, 0.157 mmol) and methylamine, 2.0 M in THF (0.79 mL, 1.57 mmol) solution. The mixture was stirred at 80° C. for 4 d. Additional methylamine, 2.0M in THF (2.0 ml) solution was added at the beginning of each day. The reaction was quenched with saturated aqueous NH4Cl solution. The crude material was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O) to afford the title compound (10.7 mg, 42%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 8.56 (q, J=4.6 Hz, 1H), 7.72 (br s, 1H), 7.56 (d, J=10.3 Hz, 1H), 7.38 (br d, J=8.3 Hz, 2H), 6.96 (d, J=8.7 Hz, 2H), 6.56 (s, 1H), 6.50 (s, 1H), 6.10 (s, 2H), 3.18-3.11 (m, 4H), 2.82 (br d, J=4.8 Hz, 5H), 2.70-2.64 (m, 1H), 2.60-2.55 (m, 4H), 1.00 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=489.43
Step 1: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-8-((2,4-dimethoxybenzyl)amino)-3,4-dihydroisoquinolin-1(2H)-one
To a stirred solution of 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.094 mmol) in N,N-dimethylformamide (DMF) (1.0 ml) was added K2CO3 (39.1 mg, 0.283 mmol) followed by 2,4-dimethoxybenzylamine (158 mg, 0.942 mmol). The reaction was heated at 80° C. for 2 days. The reaction was quenched with saturated aqueous NH4Cl solution, then the volatiles were removed in vacuo. The crude material was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-10% MeOH/DCM+1% NH4OH to give the product as a beige solid. LCMS: [M+H]+=625.61.
Step 2: 8-amino-6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of the 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-8-((2,4-dimethoxybenzyl)amino)-3,4-dihydroisoquinolin-1(2H)-one in CH2Cl2 (1.0 mL) was added TFa (215 mg, 1.89 mmol). The mixture was stirred at RT for 1 h. The mixture was concentrated onto Celite® and purified by reverse phase chromatography (C18, 0-100% MeCN—H2O). The desired fractions were then passed through an Isolute SCX-2 cation exchange resin cartridge eluting with 3% NH3 in MeOH to give the title compound (8.4 mg, 19%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.64 (br. s., 1H), 7.51 (d, J=10.2 Hz, 1H), 7.38 (d, J=8.0 Hz, 2H), 6.97 (d, J=8.8 Hz, 2H), 6.91 (br. s., 2H), 6.65 (s, 1H), 6.46 (s, 1H), 6.07 (s, 2H), 3.17 (br. s., 4H), 2.80 (t, J=6.4 Hz, 2H), 2.69-2.77 (m, 1H), 2.63 (br. s., 4H), 1.03 (d, J=6.4 Hz, 6H); LCMS: [M+H]+=475.39.
Step 1: 8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one
To a microwave vial charged with 8-bromo-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one (140 mg, 0.578 mmol), bis(pinacolato)diboron (162 mg, 0.636 mmol) and KOAc (170 mg, 1.74 mmol), was added anhydrous 1,4-dioxane (8.0 mL). The system was degassed then PdCl2dppf (42.3 mg, 0.058 mmol) was added. The mixture was flushed with nitrogen then heated at 100° C. for 2.5 h. The reaction was diluted with acetonitrile, filtered through a pad of Celite, concentrated in vacuo and used without further purification for the next step. LCMS: [M+H]+=290.35.
Step 2: 8-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one
To a solution of 5-bromo-6-fluoro-3-iodopyridin-2-amine (150 mg, 0.473 mmol) and 8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one (164 mg, 0.568 mmol) in acetonitrile (8 mL) in a microwave vial, was added a solution of Na2CO3 H2O (123 mg, 0.994 mmol) in water (2 mL). The system was degassed and bis(triphenylphosphine)palladium(II) dichloride (39.9 mg, 0.057 mmol) was added. The reaction was flushed with nitrogen and heated in the microwave at 80° C. for 3 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-100 % EtOAc/Hexanes to give the product (113 mg, 68%) as a beige solid. LCMS: [M+H]+=354.40.
Step 3: 8-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one
A procedure analogous to Example 350 using 8-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one (35 mg, 0.099 mmol), 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (39.4 mg, 0.119 mmol) and Cs2CO3 (81 mg, 0.248 mmol) in a 3:1 mixture of DME (4 mL): water (1.3 mL) and PdCl2dppf (7.27 mg, 9.94 μmol) afforded 8-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one, trifluoroacetate salt (10.3 mg, 18%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.34 (t, J=5.1 Hz, 1H), 7.85 (d, J=8.2 Hz, 1H), 7.57 (d, J=10.2 Hz, 1H), 7.39 (d, J=8.0 Hz, 2H), 7.24 (dd, J=8.1, 1.5 Hz, 1H), 7.13 (d, J=1.5 Hz, 1H), 6.96 (d, J=8.8 Hz, 2H), 6.17 (s, 2H), 4.33 (t, J=4.7 Hz, 2H), 3.35-3.38 (m, 2H), 3.12-3.17 (m, 4H), 2.65-2.71 (m, 1H), 2.56-2.60 (m, 4H), 1.01 (d, J=6.5 Hz, 6H); LCMS: [M+H]+=476.39.
Step 1: 4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)thiomorpholine 1,1-dioxide
To a round bottom flask charged with 4-(4-bromophenyl)thiomorpholine-1,1-dione (100 mg, 0.345 mmol), bis(pinacolato)diboron (96 mg, 0.379 mmol), KOAc (101 mg, 1.03 mmol), was added anhydrous 1,4-dioxane (10.0 mL). The system was degassed then PdCl2dppf (25.2 mg, 0.034 mmol) was added. The mixture was flushed with nitrogen then heated at 100° C. on the stir plate for 1.5 hours. The reaction was diluted with acetonitrile, filtered through a pad of Celite®, concentrated in vacuo and used without further purification in the next step. LCMS: [M+H]+=338.44.
Step 2: 6-(2-amino-5-(4-(1,1-dioxidothiomorpholino)phenyl)-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
A procedure similar to Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.099 mmol), 4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)thiomorpholine 1,1-dioxide (43.3 mg, 0.128 mmol), Cs2CO3 (80 mg, 0.247 mmol) in a 3:1 mixture of DME (4 mL): water (1.3 mL-) and PdCl2dppf (7.23 mg, 9.88 μmol) afforded the title compound (16.8 mg, 35%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.98 (br. s., 1H), 7.64 (d, J=10.2 Hz, 1H), 7.45 (d, J=8.1 Hz, 2H), 7.29 (s, 1H), 7.26 (d, J=12.4 Hz, 1H), 7.07 (d, J=8.9 Hz, 2H), 6.31 (s, 2H), 3.81 (br. s., 4H), 3.34 (d, J=2.9 Hz, 2H), 3.12 (d, J=4.2 Hz, 4H), 2.93 (t, J=6.2 Hz, 2H); LCMS: [M+H]+=485.36.
A procedure similar to Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.085 mmol), 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl]morpholine (30.8 mg, 0.102 mmol), Cs2CO3 (69.0 mg, 0.212 mmol) in a 3:1 mixture of DME (4 mL): water (1.3 mL) and PdCl2dppf (6.20 mg, 8.47 μmol) afforded the title compound (17.4 mg, 46%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 7.99 (br. s., 1H), 7.68 (d, J=9.9 Hz, 1H), 7.52 (d, J=7.7 Hz, 2H), 7.35 (d, J=8.1 Hz, 2H), 7.30 (s, 1H), 7.27 (d, J=12.0 Hz, 1H), 6.42 (s, 2H), 3.57 (m, 4H), 3.47 (s, 2H), 3.33-3.37 (m, 2H), 2.93 (t, J=6.1 Hz, 2H), 2.36 (br. s., 4H); LCMS: [M+H]+=451.39.
A procedure analogous To Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.085 mmol), 3-fluoro-4-morpholinophenylboronic acid (22.87 mg, 0.102 mmol), Cs2CO3 (69.0 mg, 0.212 mmol) in a 3:1 mixture of DME (4 mL): water (1.3 mL) and PdCl2dppf (6.20 mg, 8.47 μmol) afforded the title compound (19.7 mg, 51%) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 8.00 (br. s., 1H), 7.69 (d, J=10.2 Hz, 1H), 7.39 (d, J=14.4 Hz, 1H), 7.34 (d, J=8.4 Hz, H), 7.30 (s, 1H), 7.27 (d, J=12.4 Hz, 1H), 7.06 (t, J=8.9 Hz, 1H), 6.44 (s, 2H), 3.70-3.79 (m, 4H), 3.33-3.36 (m, 2H), 2.99-3.06 (m, 4H), 2.93 (t, J=6.2 Hz, 2H); LCMS: [M+H]+=455.34.
Step 1: (4-oxo-3,4-dihydroquinazolin-7-yl)boronic acid
To a round bottom flask charged with 7-bromoquinazolin-4(3 h)-one (790 mg, 3.51 mmol), bis(pinacolato)diboron (981 mg, 3.86 mmol), KOAc (1034 mg, 10.5 mmol), was added anhydrous 1,4-dioxane (40 mL). The system was degassed, then PdCl2dppf (257 mg, 0.351 mmol) was added. The system was flushed with nitrogen and heated at 100° C. for 16 h. The reaction was diluted with CH3CN, filtered through a pad of Celite, concentrated in vacuo and used as-is the next step assuming full conversion to the boronic acid. LCMS boronate: [M+H]+=273.43, LCMS boronic acid: [M+H]+=191.29.
Step 2: 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)quinazolin-4(3H)-one
To a solution of 5-bromo-6-fluoro-3-iodopyridin-2-amine (500 mg, 1.58 mmol) and (4-oxo-3,4-dihydroquinazolin-7-yl)boronic acid (10.8 ml, 1.89 mmol, 33.3 mg/mL in ACN) in acetonitrile (15 mL), in a microwave vial, was added a solution of Na2CO3 H2O (411 mg, 3.31 mmol) in water (3.75 mL). The system was degassed and bis(triphenylphosphine)palladium(II) dichloride (133 mg, 0.189 mmol) was added. The reaction was flushed with nitrogen and heated in the microwave at 80° C. for 10 h. The reaction was concentrated onto Celite® and purified by silica gel chromatography eluting with 0-100% EtOAc/hexanes to give the product (166 mg, 31%) as a beige. LCMS: [M+H]+=335.24.
Step 3: 7-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)quinazolin-4(3H)-one
A procedure similar to Example 350 using 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)quinazolin-4(3H)-one (35 mg, 0.104 mmol), 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (41.4 mg, 0.125 mmol), Cs2CO3 (85 mg, 0.261 mmol) in a 3:1 mixture of DME (4 mL): water (1.3 mL) and PdCl2dppf (7.64 mg, 10.44 μmol) afforded the title compound (15.9 mg, 33%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 12.26 (br. s., 1H), 8.17 (d, J=8.2 Hz, 1H), 8.13 (s, 1H), 7.76 (d, J=1.1 Hz, 1H), 7.65 (d, J=9.8 Hz, 2H), 7.42 (d, J=8.1 Hz, 2H), 6.98 (d, J=8.6 Hz, 2H), 6.24 (s, 2H), 3.16 (br. s., 4H), 2.89-3.01 (m, 1H), 2.63 (br. s., 4H), 1.04 (br. s., 6H); LCMS: [M+H]+=459.53.
Step 1: (1-oxo-1,2-dihydrophthalazin-6-yl)boronic acid
A procedure analogous to Example 362, Step 1 using 6-bromophthalazin-1(2H)-one (500 mg, 2.222 mmol), bis(pinacolato)diboron (621 mg, 2.444 mmol), KOAc (654 mg, 6.67 mmol), anhydrous 1,4-dioxane (40 mL) and PdCl2dppf (163 mg, 0.222 mmol) afforded the product. LCMS: Boronate: [M+H]+=273.30, LCMS Boronic acid: [M+H]+=191.23.
Step 2: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)phthalazin-1(2H)-one
A procedure analogous to Example 362, Step 2 using 5-bromo-6-fluoro-3-iodopyridin-2-amine (460 mg, 1.452 mmol) and (1-oxo-1,2-dihydrophthalazin-6-yl)boronic acid (12.8 mL, 1.89 mmol, 28.1 mg/ml in ACN) in CH3CN (15 mL) and Na2CO3 H2O (378 mg, 3.05 mmol) in water (3.75 mL) and bis(triphenylphosphine)palladium(II) dichloride (122 mg, 0.174 mmol) afforded the product (157 mg, 32%) as a beige solid. LCMS: [M+H]+=335.18.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)phthalazin-1(2H)-one
A procedure analogous to Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)phthalazin-1(2H)-one (35 mg, 0.104 mmol), 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (41.4 mg, 0.125 mmol), Cs2CO3 (85 mg, 0.261 mmol) in a 3:1 mixture of DME (4 mL): Water (1.3 mL) and PdCl2dppf (7.64 mg, 10.44 μmol) afforded the title compound (19.3 mg, 40%) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 12.65 (s, 1H), 8.38 (s, 1H), 8.26 (d, J=8.2 Hz, 1H), 8.07 (d, J=1.4 Hz, 1H), 7.96 (dd, J=8.3, 1.53 Hz, 1H), 7.69 (d, J=10.2 Hz, 1H), 7.42 (d, J=8.0 Hz, 2H), 6.97 (d, J=8.8 Hz, 2H), 6.33 (s, 2H), 3.15 (br. s., 4H), 2.65-2.72 (m, 1H), 2.59 (br. s., 4H), 1.01 (d, J=6.2 Hz, 6H); LCMS: [M+H]+=459.47.
Step 1: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)isoquinolin-1(2H)-one
A procedure analogous to Example 362 Step 2 using 5-bromo-6-fluoro-3-iodopyridin-2-amine (140 mg, 0.442 mmol) and 6-(tetramethyl-1,3,2-dioxaborolan-2-yl)-2H-isoquinolin-1-one (132 mg, 0.486 mmol) in CH3CN (5 mL) Na2CO3 H2O (115 mg, 0.928 mmol) in water (1.25 mL) and bis(triphenylphosphine)palladium(II) dichloride (37.2 mg, 0.053 mmol) afforded the product (76 mg, 52%) as a beige solid. LCMS: [M+H]+=334.24.
Step 2: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)isoquinolin-1(2H)-one
A procedure similar to Example 350 using 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)isoquinolin-1(2H)-one (30 mg, 0.090 mmol), 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (35.6 mg, 0.108 mmol), Cs2CO3 (73.1 mg, 0.224 mmol) in a 3:1 mixture of DME (4 mL): water (1.3 mL) and PdCl2dppf (6.57 mg, 8.98 μmol) afforded the title compound (15.3 mg, 37%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ 11.23 (d, J=4.3 Hz, 1H), 8.23 (d, J=8.3 Hz, 1H), 7.77 (s, 1H), 7.64 (d, J=10.2 Hz, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.41 (d, J=8.1 Hz, 2H), 7.20 (t, J=6.4 Hz, 1H), 6.97 (d, J=8.6 Hz, 2H), 6.58 (d, J=7.1 Hz, 1H), 6.20 (br. s., 2H), 3.11-3.20 (m, 4H), 2.66-2.75 (m, 1H), 2.54-2.65 (m, 4H), 1.02 (d, J=4.7 Hz, 6H); LCMS: [M+H]+=458.53.
Step 1: 6-bromo-1-methylene-2,3-dihydro-1H-indene
Methytriphenyl phosphonium bromide (101.12 g, 284.27 mmol) was dissolved in tetrahydrofuran (500 mL) followed by addition of potassium tert-butoxide (31.9 g, 284.27 mmol). Upon completion of the addition, the mixture was stirred at RT for 30 minutes. 6-Bromo-indan-1-one (20 g, 94.71 mmol) was dissolved in tetrahydrofuran (100 mL) stirring under nitrogen, treated with the ylide and the resulting mixture was stirred at RT for 1 h. The mixture was diluted with water (300 mL) and extracted with DCM (3×500 mL). The combined organic layer was back-washed with brine solution (2×500 mL), dried over Na2SO4 and concentrated under reduced pressure. The resulting crude product was purified by column chromatography over silica gel (100-200 mesh) and the product was eluted in 0-10% EtOAc in hexanes to afford the product (19 g, 95.9% yield) as a yellow oil. 1H NMR (400 MHz, Chloroform-d) δ 7.65 (s, 1H), 7.36 (d, J=7.3 Hz, 1H), 7.17 (d, J=8.1 Hz, 1H), 5.38 (s, 1H), 5.11 (s, 1H), 3.01-2.93 (m, 2H), 2.89-2.81 (m, 2H).
Step 2: 6′-bromo-2′,3′-dihydrospiro[cyclopropane-1,1′-indene
The 1 M solution of diethyl zinc in hexanes (363.6 mL, 363.3 mmol) was added to a reaction vessel containing DCM (318 mL) and the mixture was cooled to 0° C. TFA (41.4 g, 363.3 mmol) was added and the reaction was further stirred at 0° C. for 15 minutes. To the cooled solution, diiodomethane (97 g, 363.3 mmol) was added and mixture was stirred for an additional 15 minutes at 0° C. After 15 min, a solution of 6-bromo-1-methylene-2,3-dihydro-1H-indene (19 g, 90.87 mmol) in DCM (100 mL) was added and reaction mass was gradually warm to RT and stirred for 16 h. The reaction was quenched with a sat. aq. NH4Cl (500 mL) and extracted with DCM (3×300 mL). The combined organic layers were back-washed with brine solution (2×200 mL), dried over Na2SO4 and concentrated under reduced pressure. The resulting crude product was purified by column chromatography over silica gel (100-200 mesh) eluting with hexanes to afford the title compound (16 g, 78.9% yield) as a clear oil. 1H NMR (400 MHz, Chloroform-d) δ 7.22 (dd, J=7.9, 1.9 Hz, 1H), 7.05 (d, J=7.8 Hz, 1H), 6.78 (d, J=1.8 Hz, 1H), 2.99 (t, J=7.5 Hz, 2H), 2.06-2.20 (t, 2H), 0.83-1.03 (m, 5H).
Step 3: Synthesis of 6′-bromospiro[cyclopropane-1,1′-inden]-3′(2′H)-one
To a solution of 6′-bromo-2′,3′-dihydrospiro[cyclopropane-1,1′-indene (16 g, 71.71 mmol) in acetone (224 mL) and 1.5 M aq. MgSO4 (80 mL) was added KMnO4 (12.4 g, 78.5 mmol) and the reaction was stirred overnight at room temperature. After completion, the reaction was filtered over celite and concentrated. The crude was diluted with EtOAc (200 mL), washed with brine solution (2×100 mL), dried over Na2SO4 and concentrated under reduced pressure. The resulting crude product was purified by column chromatography over silica gel (100-200 mesh) eluting with 0-10% EtOAc in hexanes to give the product (10 g, 58.81%) as a white solid. LCMS: [M+2H]+=238.1.
Step 4: (Z)-6′-bromospiro[cyclopropane-1,1′-inden]-3′(2′H)-one oxime
A solution of 6′-bromospiro[cyclopropane-1,1′-inden]-3′(2′H)-one (10 g, 42.18 mmol), hydroxylamine HCl (5.5 g, 79.79 mmol) and NaOAc (20.3 g, 247.7 mmol) in MeOH (800 mL) was stirred at RT for 16 h. The reaction mixture was concentrated, slurried with water (40 mL), sonicated and filtered. The precipitate was dried under high vacuum to give the product (8 g, 75.2% yield) as a white solid. LCMS: [M+2H]+=253.1.
Step 5: (Z)-6′-bromospiro[cyclopropane-1,1′-inden]-3′(2′H)-one O-methylsulfonyl oxime
A cooled solution of (E)-6′-bromospiro[cyclopropane-1,1′-inden]-3′(2′H)-one oxime (8 g, 31.73 mmol) in THF (240 mL) at 0° C., was added triethylamine (6.4 g, 63.46 mmol) followed by slow addition of MsCI (5.4 g, 47.6 mmol). The reaction was stirred at 0° C. for 30 min, and after 30 min the reaction was concentrated and the residue was triturated with methanol (4-5 mL) and dried to afford the product (5.5 g, 52.5% yield) as white solid. LCMS: [M+2H]+=331.2.
Step 6: 6′-bromo-2′,3′-dihydro-1′H-spiro[cyclopropane-1,4′-isoquinolin]-1′-onebenzoate
To a cooled solution of (E)-6′-bromospiro[cyclopropane-1,1′-inden]-3′(2′H)-one-O-methylsulfonyl oxime (5.5 g, 16.66 mmol) in DCM (330 mL) at 0° C. was added BF3MeOH (5.2 g, 36.66 mmol). TiCl4(4.4 g, 23.33 mmol) was then added slowly and the reaction was stirred at 0° C. for 4 h. The reaction was quenched with water (50 mL) and extracted with DCM (2×100 mL). The combined organics were dried over Na2SO4 and concentrated under reduced pressure. The resulting crude product was purified by column chromatography over silica gel (100-200 mesh) eluting with 50-100% EtOAc in hexanes to afford the product (1 g, 23.8%) as white solid. LCMS: [M+2H]+=253.1.
Step 7: 6′-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2′,3′-dihydro-1′H-spiro[cyclopropane-1,4′-isoquinolin]-1′-one
Prepared from 6′-bromo-2′,3′-dihydro-1′H-spiro[cyclopropane-1,4′-isoquinolin]-1′-one (0.95 g, 3.77 mmol) to give a mixture of the boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+ 218.21 Boronic acid, 300.28 Boronate.
Step 8: 6′-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2′,3′-dihydro-1′H-spiro[cyclopropane-1,4′-isoquinolin]-1′-one (Prophetic Intermediate)
Can be prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine mmol) and 6′-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2′,3′-dihydro-1′H-spiro[cyclopropane-1,4′-isoquinolin]-1′-one in a manner analogous to Example 362, Step 2 to give the product.
Step 9: 6′-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-2′,3′-dihydro-1′H-spiro[cyclopropane-1,4′-isoquinolin]-1′-one (Prophetic)
Can be prepared from 4-(4-isopropylpiperazinyl)phenylboronic acid and 6′-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2′,3′-dihydro-1′H-spiro[cyclopropane-1,4′-isoquinolin]-1′-one in a manner analogous to Example 362 Step 3 to give the title compound.
Step 1: tert-butyl (S)-2-(5-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(tetrahydro-2H-pyran-4-yl)phenyl)pyrrolidine-1-carboxylate
A microwave vial containing a mixture of tert-butyl (S)-2-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)pyrrolidine-1-carboxylate (50 mg, 0.122 mmol, prepared as described in WO 202019351), tetrakis(triphenylphosphine)palladium(0) (16.9 mg, 0.015 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (60.7 mg, 0.158 mmol) and Na2CO3—H2O (45.3 mg, 0.366 mmol) in methyl tetrahydrofuran (4 mL) was degassed with Argon. Water (0.5 mL) was added and the reaction mixture was heated in a microwave reactor at 85° C. for 1 h. The reaction mixture was partitioned between water (3 mL) and EtOAc (3 mL), the organic phase was separated, the aqueous phase was extracted with EtOAc (3 mL), the combined organic phase was washed with brine (3 mL), dried over Na2SO4, concentrated onto Celite® and purified by column chromatography (0-100% EtOAc in hexanes) to yield the title compound (42 mg, 59%) as a beige solid. LCMS [M+H]+=587.7 HPLC purity 95%.
Step 2: (S)-6-(2-amino-6-fluoro-5-(3-(pyrrolidin-2-yl)-4-(tetrahydro-2H-pyran-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (I-367)
To a solution of tert-butyl (S)-2-(5-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(tetrahydro-2H-pyran-4-yl)phenyl)pyrrolidine-1-carboxylate (42 mg, 0.072 mmol) in DCM (2 mL), was added TFA (0.493 mL, 6.44 mmol) at RT and the reaction mixture was stirred at RT for 1.5 h. The reaction mixture was concentrated, and the residue was purified by preparatory HPLC using water (containing 0.1% formic acid)/acetonitrile (containing 0.1% formic acid) gradient. The combined fractions were mixed with 10% K2CO3 solution, extracted with CHCl3IPA (4:1) solvent mixture (x 4), the combined organic layers were dried over Na2SO4 and concentrated to yield the title compound as a white solid (18 mg, 49%). 1H NMR (500 MHz, METHANOL-d4) δ 7.94 (d, J=8.0 Hz, 1H), 7.58-7.68 (m, 1H), 7.50 (s, 1H), 7.41 (d, J=8.1 Hz, 1H), 7.30-7.38 (m, 2 H), 7.27 (d, J=8.2 Hz, 1H), 4.42 (t, J=8.0 Hz, 1H), 3.90-4.02 (m, 2H), 3.52 (t, J=11.6 Hz, 2H), 3.41-3.47 (m, 2H), 3.05-3.16 (m, 2H), 2.88-2.98 (m, 3H), 2.12-2.25 (m, 1H), 1.73-1.98 (m, 4H), 1.54-1.69 (m, 3H). LCMS: [M+H]+=487.55.
Compounds 1-368, 1-369 and 1-370 were prepared following a procedure similar to Example 366 (1-367).
Compounds 1-367 to 1-425 were prepared following procedures as outlined in the Schemes above and described in Examples 1-1 to 1-366.
Prepared from (4-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (48.3 mg, 0.238 mmol), and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (72 mg, 0.183 mmol) to afford the title compound (11.3 mg, 13% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.07 (d, J=5.50 Hz, 1H), 8.28 (d, J=8.31 Hz, 1H), 7.72 (s, 1H), 7.66 (d, J=10.15 Hz, 1H), 7.62 (dd, J=8.25, 1.28 Hz, 1H), 7.41 (d, J=7.95 Hz, 2H), 7.03 (d, J=4.89 Hz, 1H), 6.97 (d, J=8.93 Hz, 2H), 6.22 (s, 2H), 3.15 (br. s., 4H), 2.68 (br. s., 1H), 2.58 (br. s., 4H), 2.24 (s, 3H), 1.01 (d, J=6.48 Hz, 6H); LCMS: [M+H]+=472.40
Step 1: 6-bromo-4-fluoro-3-methylisoquinolin-1(2H)-one
A vial was charged with 6-bromo-3-methyl-2H-isoquinolin-1-one (1000 mg, 4.20 mmol) and Selectfluor™ fluorinating reagent >95% in F+active (1711 mg, 4.83 mmol). Methanol (15 ml) and acetonitrile (15 ml) were added, and the reaction was stirred at room temperature for 5 days. The white suspension was filtered, washing with water, and dried under vacuum to the product (903 mg, 84% yield) as a white solid. LCMS: [M+H]+=256.15.
Step 2 1-(4-Bromophenyl)-4-isopropylpiperazine
To a solution of 1-(4-bromophenyl)-piperazine (10 g, 41.4 mmol) and anhydrous acetone (2.4 g, 41.4 mmol) in ethylene dichloride (100 mL), was added NaBH(OAc)3 (13 g 62.1 mmol) and resultant reaction mass was stirred at room temperature for 1 h. After 1 h, another portion of anhydrous acetone (2.4 g, 41.4 mmol) was added and the reaction was stirred at room temperature for 16 h. After 16 h another portion of acetone (1.2 g, 20.7 mmol) was added and the reaction was further stirred for 6 h. The mixture was treated with 1N NaOH solution (100 mL) and extracted with diethyl ether (2×200 mL). The combined organic layer was washed with brine solution (100 mL), dried over anhydrous Na2SO4 and concentrated under vacuum to get crude. The crude was purified by column chromatography and the product was eluted in 4% MeOH in CH2Cl2 to afford the product (6 g, 51%) as a yellow solid. LCMS: [M+2H]+=285.27 Step 3: 6-flouro-5-iodopyridin-2-amine
A solution of 6-flouro-5-iodopyridin-2-amine (5 g, 44.59 mmol) and N-iodosuccinimide (10.88 g, 48.4 mmol) in acetonitrile (100 mL) was stirred at 0° C. for 3 h. After completion of the reaction, reaction mass was concentrated and the residue was purified by column chromatography and the product was eluted in 10% MeOH in CH2Cl2 to afford the title compound (7.5 g, 31.51 mmol, 75%) as a brownish yellow solid. LCMS: [M+H]+=239.09 Step 4: 1-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl) piperazine
1-(4-Bromophenyl)-4-isopropylpiperazine (4.3 g, 15.19 mmol) was dissolved in degassed toluene (43 mL). To this, potassium acetate (5.88 g, 60 mmol), bis(pinacolato)diboron (6.5 g, 25.67 mmol) and Pd(dppf)Cl2.DCM complex (1.22 g, 1.44 mmol) were added under nitrogen atmosphere and the reaction mass was stirred at 110° C. for 4 h. The volatiles were removed under reduced pressure to give crude product (4.2 g) as a black solid which was used as such in next step without further purification. LCMS: [M+H]+=331.46.
Step 5: 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridine-2-amine
To a degassed solution of 1-isopropyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl) phenyl) piperazine (5 g, 15.15 mmol) in dioxane (25 mL) and water (25 mL), potassium carbonate (5.2 g, 37.75 mmol) and Int-4 (3.96 g, 16.66 mmol) were added and the reaction mass was degassed for 10 min with N2. To this, tetrakis (0.875 g, 0.75 mmol) was added under nitrogen atmosphere and reaction mass was stirred at 120° C. for 4 h. After completion of reaction, the reaction mass was diluted with water (100 mL) and extracted with EtOAc (3×100 mL). The combined organic layer was dried over anhydrous Na2SO4, concentrated under vacuum. The crude material was purified by column chromatography and the product was eluted in 4% MeOH in DCM to afford the product (3.25 g, 68%) as a brown solid. LCMS: [M+H]+=315.60.
Step 6: 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyiridin-2-amine
The mixture of 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridine-2-amine (6.7 g, 21.33 mmol) and NBS (3.77 g, 21.33 mmol) in DMF (60 mL) was stirred at room temperature 6 h. To this, further NBS (1.88 g, 10.66 mmol) was added and the reaction mass was stirred at room temperature for 16 h. After 16 h, as SM was not consumed completely therefore further NBS (1.13 g, 6.4 mmol) was added and reaction mass was stirred at RT for 3 h. After completion of the reaction, the reaction mass was diluted with saturated sodium bicarbonate solution (200 mL) and product was extracted with EtOAc (3×150 mL). The combined organic was dried over anhydrous Na2SO4, concentrated under vacuum and purified by column chromatography. The product was eluted in 50-100% EtOAc in hexanes to afford the product (1.3 g, 15%) as a brownish yellow solid. LCMS: [M+2H]+=395.5.
Step 7: 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine
To 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (360 mg, 0.915 mmol), bis(pinacolato)diboron (256 mg, 1.007 mmol), and potassium acetate (269 mg, 2.75 mmol), was added 1,4-dioxane (10 ml). The system was degassed then [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (67.0 mg, 0.092 mmol) was added. The reaction was flushed with nitrogen and heated at 110° C. overnight. The mixture was diluted with acetonitrile, filtered through a pad of celite, and concentrated in vacuo to obtain a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+=441.38 Boronate, 357.45 Boronic acid.
Step 8: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 6-bromo-4-fluoro-3-methylisoquinolin-1(2H)-one (19 mg, 0.074 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (39.2 mg, 0.089 mmol) to give the title compound (3.61 mg, 9.9% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.23 (br. s., 1 H), 8.21 (dd, J=8.31, 1.59 Hz, 1H), 7.75 (s, 1H), 7.62-7.69 (m, 2H), 7.41 (d, J=7.82 Hz, 2H), 6.97 (d, J=8.80 Hz, 2H), 6.24 (br. s., 2H), 3.12-3.17 (m, 4H), 2.65-2.70 (m, 1H), 2.56-2.59 (m, 4H), 2.24 (d, J=2.93 Hz, 3H), 1.01 (d, J=6.48 Hz, 6H); LCMS [M+H]+=490.40.
Step 1: 7-chloro-2-cyclopropylquinazolin-4(3H)-one
To a solution of 2-amino-4-chlorobenzoic acid (500 mg, 2.91 mmol), 4-(dimethylamino)pyridine (35.6 mg, 0.291 mmol), triethylamine (1.625 ml, 11.66 mmol) in anhydrous 1,2-dichloroethane (6 ml) was added dropwise cyclopropanecarbonyl chloride (0.635 ml, 6.99 mmol) at 0° C. for 20 min in an ice-water batch under nitrogen. The reaction was heated at 80° C. for 3 hours. The reaction was concentrated in vacuo to remove the volatiles to give 4-chloro-2-(cyclopropanecarboxamido)benzoic acid which was used in the next step as-is. LCMS: [M−H]−=238.00. 4-Chloro-2-(cyclopropanecarboxamido)benzoic acid was taken up in 27% ammonium hydroxide solution (8.41 ml, 58.3 mmol) and heated in a sealed tube at 80° C. overnight. The mixture was cooled to RT and the solid was filtered, washed with water and dried under vacuum to afford the product (535 mg, 83% yield) as a beige solid. LCMS: [M+H]+=221.10.
Step 2: (2-cyclopropyl-4-oxo-3,4-dihydroquinazolin-7-yl)boronic acid
Prepared from 7-chloro-2-cyclopropylquinazolin-4(3H)-one (72 mg, 0.326 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+=313.38 Boronate, 231.32 Boronic acid.
Step 3: 7-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-2-cyclopropylquinazolin-4(3H)-one
Prepared from (2-cyclopropyl-4-oxo-3,4-dihydroquinazolin-7-yl)boronic acid (35.1 mg, 0.153 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (50 mg, 0.127 mmol) to give the title compound (2.7 mg, 4.3% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 12.43 (s, 1H), 8.10 (d, J=8.19 Hz, 1H), 7.62 (d, J=10.15 Hz, 1H), 7.56 (s, 1H), 7.52 (d, J=8.19 Hz, 1H), 7.43 (br. s., 2H), 6.98 (br. s., 2H), 6.18 (br. s., 1H), 3.14 (br. s., 4H), 1.98 (d, J=4.52 Hz, 1H), 1.11 (br. s., 4H), 1.05 (d, J=7.58 Hz, 6 H); LCMS: [M+H]+=499.36.
Step 1: 7-chloro-2-cyclobutylquinazolin-4(3H)-one
To a solution of 2-amino-4-chlorobenzamide (200 mg, 1.172 mmol), triethylamine (0.487 ml, 3.50 mmol) in anhydrous dichloromethane (2.0 ml) was added dropwise cyclobutanecarbonyl chloride (0.319 ml, 2.80 mmol). The mixture was stirred at room temperature for 2 hours then heated at 40° C. for 1 hour. The reaction was concentrated in vacuo and 5% NaOH(aq) (3 ml) was added. The reaction was heated at 90° C. overnight. The reaction was cooled to RT and 1N HCl(aq) was added until ˜ pH 7. The mixture was concentrated onto celite and purified by flash chromatography (Biotage, silica gel, 0-100% EtOAc/Hexanes). The desired fractions were collected, concentrated and dried under vacuum to obtain the product (96 mg, 0.409 mmol, 35.1% yield) as a white solid. LCMS: [M+H]+=235.02.
Step 2: 2-cyclobutyl-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)quinazolin-4(3H)-one
The title compound was prepared from 7-chloro-2-cyclobutylquinazolin-4(3H)-one (54 mg, 0.230 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS [M+H]+ 327.43 Boronate, 245.24 Boronic acid.
Step 3: 7-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-2-cyclobutylquinazolin-4(3H)-one
Prepared from 2-cyclobutyl-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)quinazolin-4(3H)-one (60.7 mg, 0.186 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (61 mg, 0.155 mmol) to give the title compound (12.47 mg, 0.024 mmol, 15.68%) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 12.06 (s, 1H), 8.12 (d, J=8.07 Hz, 1H), 7.73 (s, 1H), 7.65 (d, J=10.15 Hz, 1H), 7.58 (dd, J=8.07, 1.22 Hz, 1H), 7.43 (d, J=8.19 Hz, 2H), 6.97 (d, J=8.56 Hz, 2H), 6.21 (s, 2H), 3.52 (quin, J=8.56 Hz, 1H), 3.15 (br. s., 4H), 2.68 (br. s., 1H), 2.59 (br. s., 4H), 2.38-2.47 (m, 2H), 2.22-2.31 (m, 2H), 1.95-2.04 (m, 1H), 1.85 (q, J=9.62 Hz, 1H), 1.02 (d, J=4.28 Hz, 6H); LCMS [M+H]+=513.40.
Step 1: 7-bromo-2-methoxyquinazolin-4(3H)-one
To a solution of 7-bromo-2-chloroquinazolin-4(3H)-one (100 mg, 0.385 mmol) in tetrahydrofuran (1.0 ml) was added sodium methoxide, 0.5 M in MeOH (1.2 ml, 0.578 mmol) and heated at 90° C. for 5 days. The reaction was quenched with saturated aqueous NH4Cl solution and then concentrated onto celite. The crude mixture was purified by flash chromatography (Biotage, silica gel) eluting with 0-100% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum to afford the product (12 mg, 12% yield) as a white solid. LCMS [M+H]+ 255.08.
Step 2: 7-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-2-methoxyquinazolin-4(3H)-one
The title compound was prepared from 7-bromo-2-methoxyquinazolin-4(3H)-one (12 mg, 0.047 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (24.86 mg, 0.056 mmol) to give the title compound (2.2 mg, 9.6% yield) as a white solid. 1 H NMR (500 MHz, DMSO-d6) δ ppm 7.98 (d, J=8.07 Hz, 1H), 7.59 (d, J=10.15 Hz, 1H), 7.42 (br. s., 1H), 7.40 (s, 2H), 7.26 (br. s., 1 H), 6.96 (d, J=8.80 Hz, 2H), 6.08 (br. s., 2H), 3.84 (s, 3H), 3.10-3.19 (m, 4H), 2.68 (d, J=6.48 Hz, 1H), 2.56-2.59 (m, 4H), 1.01 (d, J=6.60 Hz, 6H); LCMS: [M+H]+=489.39.
Prepared from 2-amino-7-bromo-3H-quinazolin-4-one (35 mg, 0.146 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (77 mg, 0.175 mmol) to give the title compound (18.4 mg, 26.7%) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.35 (br. s., 1H), 7.93 (d, J=8.07 Hz, 1H), 7.58 (d, J=10.15 Hz, 1H), 7.40 (d, J=8.07 Hz, 2H), 7.25 (s, 1H), 7.17 (d, J=8.07 Hz, 1H), 6.96 (d, J=8.80 Hz, 2H), 6.56 (br. s., 2H), 6.11 (br. s., 2H), 3.12-3.17 (m, 4H), 2.64-2.70 (m, 1H), 2.55-2.60 (m, 4H), 1.01 (d, J=6.48 Hz, 6H); LCMS: [M+H]+=474.41.
Step 1 (E)-3-(3-bromophenyl)but-2-enoic acid
To THF (150 mL) at 0° C. under nitrogen atmosphere, sodium hydride (60%, 6 g, 150.71 mmol) was add portion wise. To this, triethyl phosphonoacetate (21.47 mL, 113.06 mmol) was added dropwise and stirred at 0° C. for 10 min. The resulting reaction mass was added dropwise to a solution of 1-(3-bromophenyl)ethan-1-one (15 g, 75.35 mmol) in THF (150 mL) and the reaction mass was reflux for 16 h. After completion of the reaction, reaction mass was diluted with water (500 mL) and extracted with DCM (3×500 mL). The organic layer was dried over anhydrous Na2SO4 and concentrated under vacuum to afford a yellow oil. The oil was dissolved in methanol (150 mL) and sodium hydroxide (9.04 g, 226.13 mmol) and water (75 mL) were added and the reaction mass was heated at 50° C. for 2 h. After completion of the reaction, the reaction mass was concentrated to evaporate the organics. The aqueous solution was acidified by aqueous 2M HCl and extracted with ethyl acetate (2×500 mL). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under vacuum to afford the product (7 g, 39%) as yellow solid LCMS: [M−2H]+=238.9.
Step 2: (E)-3-(3-bromophenyl)but-2-enoyl chloride
To a cooled solution of (E)-3-(3-bromophenyl)but-2-enoic acid (6 g, 24.88 mmol) in DCM (60 mL) and DMF (0.2 mL) at 0° C., oxaloyl chloride (2.59 mL, 29.86 mmol) was added dropwise and the reaction mass was allowed to warm to RT and stirred at same temperature for 3 h. After completion the reaction, reaction mass was concentrated and azeotrope with toluene (2×20 mL) and DCM (2×20 mL) to get the crude product which was used as is in the next step.
Step 3: (E)-3-(3-bromophenyl)but-2-enoyl azide
To a cooled solution of crude of (E)-3-(3-bromophenyl)but-2-enoyl chloride (6 g, 23.11 mmol) in 1,4 dioxane (60 mL) at 0° C., a suspension of sodium azide (2.26 g, 34.47 mmol) in 1:1 mixture of 1,4-dioxane and water (20 mL) was added and the reaction mas was gradually warm to room temperature and stirred at same temperature for a 3 h. After completion reaction, reaction mass was diluted with water (70 mL) and extracted with diethyl ether (2×200 mL). The combined organic mixture was back washed with saturated sodium bicarbonate solution (3×200 mL) and water (3×200 mL) and dried over anhydrous Na2SO4 and organic layer was directly used for next step.
Step 4: 6-bromo-4-methylisoquinolin-1(2H)-one
To the ether layer of (E)-3-(3-bromophenyl)but-2-enoyl azide is treated with 1,2 dichlorobenzene (15 mL) and the ether was removed under vacuum to give a solution of (E)-3-(3-bromophenyl)but-2-enoyl azide in 1,2 dichlorobenzene. The acyl azide solution in 1,2 dichlorobenzene was added dropwise over 30 min to a solution of iodine (3 crystal) in 1,2 dichlorobenzene (15 mL) at 120° C. After completion of addition, reaction mass was stirred at 190° C. for 16 h. After completion of the reaction, reaction mass was allowed to cool room temperature and added to hexane (800 mL). The suspension stirred for 1 h and the solid thus obtained was filtration, washed with ethyl acetate (20 mL) and DCM (20 mL) and dried under vacuum to give the product (1.25 g, 23%) as pale yellow solid. LCMS: [M+2]+=240.8
Step 5: 4-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one
To 6-bromo-4-methylisoquinolin-1(2H)-one (742 mg, 3.12 mmol), bis(pinacolato)diboron (871 mg, 3.43 mmol), and potassium acetate (918 mg, 9.35 mmol), was added anhydrous 1,4-dioxane (20 ml). The system was flushed with nitrogen then [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (228 mg, 0.312 mmol) was added. The mixture was flushed with nitrogen then heated at 100° C. for 2 hours. The reaction was diluted with acetonitrile, filtered through a pad of celite, concentrated in vacuo and used crude in the next step (120 mg, 0.341 mmol, 100%) as a mixture of boronate and boronic acid. LCMS: [M+H]+=285.99 Boronate, 204.29 Boronic acid
Step 6: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
To 5-bromo-6-fluoro-3-iodopyridin-2-amine (405 mg, 1.278 mmol) and 4-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one (437 mg, 1.534 mmol) in acetonitrile (10 ml) was added a solution of sodium carbonate monohydrate (333 mg, 2.68 mmol) in water (2.5 mL). The system was degassed and bis(triphenylphosphine)palladium(II) dichloride (108 mg, 0.153 mmol) was added. The reaction was flushed with nitrogen then heated at 90° C. overnight. The reaction was concentrated onto celite and purified by flash chromatography (Biotage, silica gel) eluting with 0-100% EtOAc/Hexanes. The desired fractions were collected, concentrated, triturated from ether and dried under vacuum to afford 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (178 mg, 0.363 mmol, 28.4% yield) as a grey solid. LCMS [M +H]+=348.18.
Step 7: Methyl 2-(3,6-dihydro-2H-pyran-4-yl)-5-nitrobenzoate
To a degassed solution of methyl 2-iodo-5-nitrobenzoate (4 g, 13.02 mmol), 2-(3,6-dihydro-2H-pyran-4-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (3.4 g, 13.15 mmol) and K2CO3 (4.5 g, 32.56 mmol) in dioxane: water (7.5: 2.5, 40 mL), Pd(dppf)Cl2.DCM (1.06 g, 1.3 mmol) was added and further degassed with N2 for 10 minutes and the reaction mass was stirred at 90° C. for 16 h. After completion of reaction, reaction mass was diluted with water (40 mL) and extracted with EtOAc (3×30 mL). The crude was purified by column chromatography and the product was eluted in 18% EtOAc in hexanes to afford the product (3.1 g, 90%) as off-white solid. 1H NMR (400 MHz, Chloroform-d) δ 8.74 (d, J=2.4 Hz, 1H), 8.47 (s, 1H), 8.35 (dd, J=8.4, 2.5 Hz, 1H), 7.46 (d, J=8.4 Hz, 1H), 5.7 (s, 1H), 4.35 (q, J=2.7 Hz, 2H), 3.98 (d, J=6.6 Hz, 5H), 2.41 (tq, J=4.9, 2.3 Hz, 2H).
Step 8: Methyl 5-amino-2-(tetrahydro-2H-pyran-4-yl)benzoate
In a 3 neck RBF, 10% Pd/C (50% moist, 2w/w, 7.6 g) and methyl 2-(3,6-dihydro-2H-pyran-4-yl)-5-nitrobenzoate (3.8 g, 14.43 mmol) were added in EtOAc (40 mL). The reaction mass was stirred at room temperature with continuous purging of hydrogen for 2.5 days. After completion of reaction, reaction mass was filtered through celite bed and the filtrate was concentrated under vacuum to afford the crude product (3.2 g, 94%) as pale yellow gummy solid used as such in next step without further purification. LCMS [M+H]+=236.5.
Step 9: Methyl 5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzoate
To a solution of methyl 5-amino-2-(tetrahydro-2H-pyran-4-yl)benzoate (3 g, 12.74 mmol) in bromoform (9.6 mL). To this, tert-butylnitrite (15 mL) was added dropwise at room temperature and the reaction mass was stirred at same temperature for 30 minutes. After completion of reaction, the reaction mass was concentrated to afford crude. The crude was purified by column chromatography and the product was eluted in 10% EtOAc in hexanes to afford the product (1.8 g, 47%) as a yellow solid. 1H NMR (400 MHz, Chloroform-d) 1.76-1.90 (m, 4H), 3.63 (m, 4H), 3.95 (s, 3H), 4.11 (dt, J=11.1, 2.9 Hz, 1H), 7.32 (d, J=8.4 Hz, 1H), 7.64 (dd, J=8.4, 2.3 Hz, 1H), 7.98 (d, J=2.4 Hz, 1H).
Step 10: (5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)methanol
To a cooled solution of methyl 5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzoate (1.8 g, 6.02 mmol) in dry THF (18 mL) at 0° C., LAH (1 M in THF, 6.12 mL, 6.02 mmol) was added dropwise at 0° C. The reaction mass was stirred at RT for 3 h. After completion of reaction, reaction mass was quenched with the dil. HCl (50 mL) and extracted with ethyl acetate (3×50 mL). The combined organic was dried over anhydrous Na2SO4, concentrate under vacuum to afford the title compound (1.3 g, 4.79 mmol, 79.68%) as pale-yellow liquid. 1H NMR (400 MHz, Chloroform-d) 1.67-1.90 (m, 4H), 3.05 (m, 1H), 3.58 (m, 2H), 4.10 (d, J=11.1, 1H), 4.73 (s, 2H), 7.21 (d, J=8.4 Hz, 1H), 7.47 (dd, J=8.4, 2.3 Hz, 1H), 7.55 (d, J=2.4 Hz, 1H).
Step 11: 5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzaldehyde
To a solution of (5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)methanol (1.3 g, 4.79 mmol) in DCM (26 mL), MnO2 (3.3 g, 38.35 mmol) was added at room temperature and the reaction mass was stirred at room temperature for 48 h. After completion of reaction, reaction mass was filtered through celite bed and the filtrate was concentrate under vacuum to afford crude product. The crude was purified by column chromatography and the product was eluted in 15% EtOAc in hexanes to afford the product (0.76 g, 58.9%) as light-yellow solid. 1H NMR (400 MHz, Chloroform-d) δ 1.76-1.84 (m, 2H), 1.86-1.93 (m, J=12.2, 4.3 Hz, 2H), 3.61-3.67 (t, J=11.7 Hz, 2H), 3.89-3.83 (tt, J=3.2, 4.0 Hz, 1H), 4.15-4.12 (dd, J=11.4, 4.6 Hz, 2H), 7.39-7.37 (t, J=8.4 Hz, 1H), 7.77 (d, 1H), 7.98 (s, 1H), 10.27 (s, 1H).
Step 12: 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)-N,N-dimethylmethanamine
To a solution of 5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzaldehyde (400 mg, 1.486 mmol) in dichloromethane (DCM) (10 ml) was added dimethylamine, 2.0M solution in THF (2.229 ml, 4.46 mmol) followed by acetic acid, glacial, 99.8% (0.00850 ml, 0.149 mmol). Upon stirring at room temperature for 10 minutes, sodium triacetoxyborohydride (945 mg, 4.46 mmol) was added portion-wise and the white suspension was stirred at room temperature for 1 hour. The reaction was basified with 1M NaOH(aq) solution. The organic phase was separated, and the aqueous phase was further washed with DCM (2x). The combined organic phases were washed with brine (1x), dried over anhydrous sodium sulfate and concentrated in vacuo to obtain 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)-N,N-dimethylmethanamine (394 mg, 1.321 mmol, 89% yield) as a white solid. The material was carried onto the next step without further purification. LCMS [M+H]+298.34.
Step 13: N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine
To a vial charged with 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)-N,N-dimethylmethanamine (385 mg, 1.291 mmol), bis(pinacolato)diboron (361 mg, 1.420 mmol), and potassium acetate (380 mg, 3.87 mmol), was added anhydrous 1,4-dioxane (5 ml). The system was degassed then [1,12-bis(diphenylphosphino)ferrocene] dichloropalladium(II) (94 mg, 0.129 mmol) was added. The reaction was flushed with nitrogen and heated at 100° C. overnight. The mixture was diluted with acetonitrile, filtered through a pad of celite, concentrated in vacuo and used as-is the next step assuming full conversion to the boronate. LCMS [M+H]+=346.55.
Step 14: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(tetrahydro-2H-pyran-4-yl)phenyl)-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
To 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (35 mg, 0.101 mmol), N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (41.7 mg, 0.121 mmol), Cs2CO3 (98 mg, 0.302 mmol) in a mixture of 1,4-dioxane (5 ml): water (1 ml) was added tetrakis(triphenylphosphine)palladium(0) (13.94 mg, 0.012 mmol). The reaction was flushed with nitrogen and heated at 90° C. for 3 hours. The mixture was concentrated onto celite and purified by flash chromatography to afford the title compound (25.6 mg, 52.3% yield) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.10 (d, J=5.01 Hz, 1H), 8.28 (d, J=8.19 Hz, 1H), 7.72 (d, J=0.98 Hz, 1H), 7.70 (d, J=10.03 Hz, 1H), 7.62 (dd, J=8.31, 1.34 Hz, 1 H), 7.44 (d, J=8.19 Hz, 1H), 7.39 (s, 1H), 7.34 (d, J=8.07 Hz, 1H), 7.04 (d, J=4.52 Hz, 1H), 6.34 (s, 2H), 3.95 (dd, J=10.88, 3.42 Hz, 2H), 3.41-3.47 (m, 4H), 3.16-3.23 (m, 1H), 2.24 (s, 3H), 2.16 (s, 6H), 1.70 (qd, J=12.25, 4.10 Hz, 2H), 1.58-1.65 (m, 2H); LCMS [M+H]+=487.45.
Step 1: Preparation of 6-bromo-3-morpholinopicolinaldehyde
Prepared by reacting 2-bromo-5-fluoro-6-formylpyridine (2.5 g, 12.25 mmol) and morpholine (1.269 mL, 14.71 mmol) at 85° C. for 6 h. The product was isolated as a pale-yellow oil (2.75 g, 79% yield). LCMS: [M+H]+=271.1.
Step 2: 1-(6-bromo-3-morpholinopyridin-2-yl)-N,N-dimethylmethanamine
Prepared using 6-bromo-3-morpholinopicolinaldehyde (500 mg, 1.752 mmol) and dimethylamine, 2.0M solution in THF (0.876 mL, 1.752 mmol). Silica gel chromatography afforded the product as a cream colored solid (336 mg, 62% yield). LCMS: [M+H]+=300.35.
Step 3: 6-(6′-amino-6-((dimethylamino)methyl)-2′-fluoro-5-morpholino-[2,3′-bipyridin]-5′-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared as per previous procedures from the intermediate 1-(6-bromo-3-morpholinopyridin-2-yl)-N,N-dimethylmethanamine (50 mg, 0.162 mmol) from with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (85 mg, 0.211 mmol) to yield the title compound as a white solid (48 mg, 57% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.14-8.11 (m, 1H), 8.09-8.01 (m, 1H), 7.68-7.64 (m, 1H), 7.64-7.61 (m, 1H), 7.58-7.51 (m, 1H), 7.43-7.40 (m, 1H), 6.57-6.37 (m, 2H), 3.80-3.73 (m, 4H), 3.53 (br s, 2H), 3.45-3.40 (m, 2H), 3.05 (br s, 4H), 2.96-2.91 (m, 2H), 2.24 (s, 6H). LCMS: [M+H]+=495.54
Step 1: Preparation of 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)-4-methoxypiperidine
Prepared by a procedure analogous the previous example using 5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzaldehyde (400 mg, 1.486 mmol) and N-ethylmethylamine (0.511 ml, 5.94 mmol) as the amine. The product was isolated as a white solid (444 mg, 91 % yield). LCMS: [M+H]+=312.38.
Step 2: Preparation of 6-(2-amino-5-(3-((ethyl(methyl)amino)methyl)-4-(tetrahydro-2H-pyran-4-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared by coupling the intermediate N-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)-N-methylethanamine (55 mg, 0.167 mmol) with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (83 mg, 0.218 mmol) to yield the title compound as a white solid (65 mg, 76% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.00-7.93 (m, 1H), 7.93-7.88 (m, 1H), 7.66-7.60 (m, 1H), 7.49-7.46 (m, 1H), 7.46-7.41 (m, 2H), 7.41-7.37 (m, 1H), 7.37-7.32 (m, 1H), 6.37-6.16 (m, 2H), 4.02-3.94 (m, 2H), 3.54-3.48 (m, 2H), 3.46-3.39 (m, 4H), 3.27-3.21 (m, 1H), 2.99-2.93 (m, 2H), 2.43-2.37 (m, 2H), 2.13-2.05 (m, 3H), 1.75-1.66 (m, 2H), 1.66-1.59 (m, 2H), 1.09-1.02 (m, 3H). LCMS: [M+H]+=489.52Example 371: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(tetrahydro-2H-pyran-4-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (I-429)
Prepared from 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)-N,N-dimethylmethanamine (60 mg, 0.157 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (63 mg, 0.157 mmol) to afford the title compound as a white solid (24 mg, 30% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.63 (d, J=10.03 Hz, 1H), 7.54-7.57 (m, 1H), 7.33-7.36 (m, 1H), 7.28-7.32 (m, 2H), 7.26 (d, J=8.19 Hz, 1H), 3.94 (dd, J=11.19, 3.73 Hz, 2H), 3.41-3.52 (m, 6H), 3.10-3.17 (m, 1H), 2.91 (t, J=6.60 Hz, 2H), 2.12-2.21 (m, 6H), 1.68-1.80 (m, 2H), 1.60 (d, J=12.96 Hz, 2H). LCMS: [M+H]+=493.34.
Step 1: tert-Butyl 7-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,7-diazaspiro[3.5]nonane-2-carboxylate
4-Bromophenylboronic acid, pinacol ester (813 mg, 2.87 mmol), sodium tert-butoxide(318 g, 3.31 mmol) tert-butyl 2,7-diazaspiro[3.5]nonane-2-carboxylate (500 mg, 2.21 mmol), 2-dicyclohexylphosphino-2′,6′-di-i-propoxy-1,1′-biphenyl (10.3 mg, 0.022 mmol) and chloro-(2-Dicyclohexylphosphino-2′,6′-diisopropoxy-1,1′-biphenyl)[2-(2-aminoethyl)phenyl]palladium(II)—methyl-t-butyl ether adduct (18.1 mg, 0.0.22 mmol) were mixed together in 1,4-Dioxane (8 mL) under vacuum. The reaction mixture was stirred at r.t for 2 days. The reaction mixture was diluted with EtOAc, washed with Brine, concentrated with silica gel and purified by column chromatography, eluted with EtOAc to give the product (572 mg, 60.4% yield) as off-white solid. LCMS: [M+H]+=429.29.
Step 2: 7-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,7-diazaspiro[3.5]nonane dihydrochloride
tert-Butyl 7-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,7-diazaspiro[3.5]nonane-2-carboxylate (543 mg, 1.268 mmol) was mixed with ether (5 mL) and MeOH (5 mL) at r.t., then Hydrogen chloride solution 4.0 M in dioxane (3.17 mL, 12.68 mmol) were added. The reaction mixture was stirred at r.t. overnight. The reaction mixture was concentrated by rotavapor. The residue was triturated with EtOAc to give the product (500 mg, 98% yield) as white solid. LCMS: [M+H]+=329.43.
Step 3: 2-methyl-7-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,7-diazaspiro[3.5]nonane
Prepared from 7-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,7-diazaspiro[3.5]nonane dihydrochloride (180 mg, 0.449 mmol) reacted with Formaldehyde solution, 37% wt in water (54.6 mg, 0.673 mmol) and Sodium triacetoxyborohydride (143 mg, 0.673 mmol), sodium acetate (73.6 mg, 0.897 mmol) in DCM (2.5 mL) and MeOH (0.5 mL) at r.t. for an hour to give after standard workup the product (143 mg, 93% yield). LCMS: [M+H]+=343.41;
Step 4 6-(2-amino-6-fluoro-5-(4-(2-methyl-2,7-diazaspiro[3.5]nonan-7-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-6-fluoro-5-(4-(2-methyl-2,7-diazaspiro[3.5]nonan-7-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one was prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.119 mmol) and 2-methyl-7-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,7-diazaspiro[3.5]nonane (33.9 mg, 0.099 mmol), to give the title compound (29.5 mg, 60% yield). 1H NMR (500 MHz, DMSO-d6) δ=7.93 (br s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.57 (d, J=10.1 Hz, 1H), 7.48-7.42 (m, 2H), 7.38 (br d, J=7.9 Hz, 2H), 6.96 (d, J=8.8 Hz, 2H), 6.16 (s, 2H), 3.44-3.38 (m, 2H), 3.15-3.08 (m, 4H), 2.99-2.89 (m, 6H), 2.22 (s, 3H), 1.78-1.72 (m, 4H); LCMS: [M+H]+=472.41.
Prepared from 7-bromo-5-methyl-1,2,3,4-tetrahydroisoquinoline (40 mg, 0.177 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (85 mg, 0.221 mmol) to yield the title compound as a beige solid (65 mg, 87% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.05 (d, J=7.95 Hz, 1H), 7.62 (d, J=9.66 Hz, 1H), 7.51 (d, J=7.95 Hz, 1H), 7.46 (s, 1H), 7.20 (s, 1H), 7.07 (s, 1H), 3.99 (s, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.16 (t, J=6.05 Hz, 2H), 3.02-3.10 (m, 2H), 2.67-2.80 (m, 2H), 2.24-2.33 (m, 3H). LCMS: [M+H]+=403.34.
Step 1: 1-(5-bromo-2-morpholinophenyl)-N,N-dimethylmethanamine
Dimethylamine, 2.0M solution in THF (3.70 ml, 7.40 mmol) was added to a solution of 5-bromo-2-morpholinobenzaldehyde (500 mg, 1.85 mmol), and glacial acetic acid (10.59 μl, 0.185 mmol) in CH2Cl2 (10 mL) at RT. After 5 min of stirring NaBH(OAC)3 (1569 mg, 7.40 mmol) was added and the suspension was stirred for 30 min at RT. The reaction mixture was basified with 1 N NaOH aqueous solution. The organic phase was separated, and aqueous phase was extracted with CH2Cl2 (5 mL). The combined org phase was dried over Na2SO4 and concentrated to yield the crude product as a pale-yellow solid (543 mg, 98%). LCMS: [M+H]+=299.92.
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-morpholinophenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(5-bromo-2-morpholinophenyl)-N,N-dimethylmethanamine (60 mg, 0.201 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.261 mmol) to yield the title compound as a beige solid (63 mg, 63% yield). 1H NMR (500 MHz, Methanol-d4) δ ppm 7.92-7.97 (m, 1H), 7.57-7.63 (m, 1H), 7.48-7.52 (m, 1H), 7.48-7.51 (m, 1H), 7.41 (dd, J=8.07, 1.59 Hz, 1H), 7.34-7.39 (m, 2H), 7.13 (d, J=8.44 Hz, 1H), 3.73-3.79 (m, 4H), 3.53-3.59 (m, 2H), 3.45 (t, J=6.66 Hz, 2H), 2.96 (t, J=6.66 Hz, 2H), 2.79-2.87 (m, 4H), 2.19 (s, 6H). LCMS: [M+H]+=476.42.
Prepared from 1-(5-bromo-2-morpholinophenyl)-N,N-dimethylmethanamine (60 mg, 0.201 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (105 mg, 0.261 mmol) to afford the title compound as a beige solid (46 mg, 44% yield). 1H NMR (500 MHz, Methanol-d4) δ ppm 8.09 (br. s., 1H), 7.60 (dd, J=12.96, 10.15 Hz, 2H), 7.50 (s, 1H), 7.41 (dd, J=16.44, 7.76 Hz, 2H), 7.12 (d, J=8.44 Hz, 1H), 6.25 (s, 2H), 3.70-3.78 (m, 4H), 3.44 (s, 2H), 3.40 (td, J=6.42, 2.69 Hz, 2H), 2.89-2.97 (m, 6H), 2.18 (s, 6H). LCMS: [M+H]+=494.41.
Step 1: 7-bromo-2,5-dimethyl-1,2,3,4-tetrahydroisoquinoline
Prepared using 7-bromo-5-methyl-1,2,3,4-tetrahydroisoquinoline (40 mg, 0.177 mmol) and formaldehyde solution, 37% wt in water (0.040 mL, 0.531 mmol) to give the product as a pale-yellow solid (38 mg, 89% yield). LCMS: [M+H]+=239.96.
Step 2: Preparation of 6-(2-amino-5-(2,5-dimethyl-1,2,3,4-tetrahydroisoquinolin-7-yl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 7-bromo-2,5-dimethyl-1,2,3,4-tetrahydroisoquinoline (38 mg, 0.158 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (76 mg, 0.198 mmol) to afford the title compound as an off white solid (36 mg, 52% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.01-8.10 (m, 1H), 7.60-7.67 (m, 1H), 7.51 (dd, J=7.95, 1.47 Hz, 1H), 7.46 (s, 1H), 7.21 (s, 1H), 7.09 (s, 1H), 3.65 (s, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.07 (t, J=6.66 Hz, 2H), 2.83 (dd, J=11.00, 4.65 Hz, 4H), 2.48 (s, 3H), 2.28 (s, 3H); LCMS: [M+H]+=417.44.
Step 1: 7-chloro-2-(trifluoromethyl)quinazolin-4(3H)-one
To a 0° C. solution of 2-amino-4-chlorobenzoic acid (300 mg, 1.748 mmol) and N,N-diisopropylethylamine (1.523 ml, 8.74 mmol) in 1,2-dichloroethane (5 ml) was added trifluoroacetic anhydride (0.729 ml, 5.25 mmol). After addition, the reaction was heated at 50° C. for 1 hour. The reaction was concentrated in vacuo and ammonia solution, 7 N in methanol (2.498 ml, 17.48 mmol) was added. The reaction was heated at 80° C. overnight. The reaction was concentrated onto celite and purified by flash chromatography (Biotage, silica gel) eluting with 0-100% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum to afford the product (66.7 mg, 15% yield) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 13.74 (br. s., 1H), 8.18 (d, J=8.56 Hz, 1H), 7.92 (d, J=1.96 Hz, 1H), 7.71 (dd, J=8.50, 2.02 Hz, 1H); LCMS: [M+H]+=249.19.
Step 2: 7-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-2-(trifluoromethyl)quinazolin-4(3H)-one
The title compound was prepared from 7-chloro-2-(trifluoromethyl)quinazolin-4(3H)-one (43 mg, 0.147 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (78 mg, 0.176 mmol) to give the title compound (2.2 mg, 4.20 μmol, 2.86% yield) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.17 (d, J=8.07 Hz, 1H), 7.80 (br. s., 1H), 7.66 (d, J=10.03 Hz, 2H), 7.46 (d, J=8.07 Hz, 2H), 7.01 (d, J=8.68 Hz, 2H), 6.24 (br. s., 2H), 2.95 (br. s., 4H), 1.15 (d, J=3.42 Hz, 6H); LCMS: [M+H]+=527.31.
Prepared from N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (61 mg, 0.176 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.141 mmol) to yield the title compound as an off white solid (46 mg, 63% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.72 (d, J=9.66 Hz, 1H), 7.48 (d, J=8.19 Hz, 1H), 7.46 (s, 1H), 7.39 (d, J=8.07 Hz, 1H), 7.32 (s, 1H), 7.28 (d, J=11.98 Hz, 1H), 4.07 (dd, J=11.19, 3.73 Hz, 2H), 3.61 (t, J=11.19 Hz, 2H), 3.57 (s, 2H), 3.51 (t, J=6.42 Hz, 2H), 3.23-3.30 (m, 1H), 3.06 (t, J=6.42 Hz, 2H), 2.25-2.36 (m, 6H), 1.81-1.93 (m, 2H), 1.73 (d, J=12.59 Hz, 2H); LCMS: [M+H]+=493.34.
Prepared from N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (58 mg, 0.168 mmol) and 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2-methylquinazolin-4(3H)-one (60 mg, 0.134 mmol) to yield the title compound as a beige solid (32 mg, 46.5% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.29 (d, J=8.19 Hz, 1H), 7.73-7.79 (m, 2H), 7.72-7.79 (m, 2H), 7.66 (dd, J=8.19, 1.59 Hz, 1H), 7.45-7.53 (m, 2H), 7.45-7.53 (m, 2H), 7.40 (d, J=8.19 Hz, 1H), 4.07 (dd, J=11.13, 3.79 Hz, 2H), 3.61 (t, J=11.00 Hz, 2H), 3.55-3.58 (m, 2H), 3.57 (s, 2H), 3.24-3.30 (m, 1H), 2.49 (s, 3H), 2.28-2.32 (m, 6H), 1.81-1.92 (m, 2H), 1.73 (d, J=11.49 Hz, 2H). LCMS: [M+H]+=488.33.
Step 1: Preparation of 7-bromo-6-methoxy-2-methyl-1,2,3,4-tetrahydroisoquinoline
To an acetonitrile (12 mL) solution of 6-methoxy-2-methyl-1,2,3,4-tetrahydroisoquinolin-7-amine (200 mg, 1.04 mmol), copper(I) bromide (14.92 mg, 0.104 mmol), tert-butyl nitrite, >=90% (GC) (129 mg, 1.248 mmol) and tetrabutylammonium bromide (671 mg, 2.08 mmol) at RT was added camphor-10-sulfonic acid, 98% (290 mg, 1.25 mmol) and the reaction mixture was stirred at RT for 2 h. The reaction mixture was quenched with 2 ml water and the volatiles were removed. The residue was partitioned between 5 ml water and 4 ml CH2Cl2, the organic phase was separated, aqueous phase was extracted with CH2Cl2 (3×4 mL), the combined org phase was washed with brine, dried over Na2SO4 and concentrated. Purification by silica gel chromatography, eluting with CH2Cl2 containing 0-2% MeOH and 0-0.2% NH4OH, followed by filtration through a porapak cation exchange resin cartridge yielded the title compound as a brown oil (98 mg, 18% yield, 50% purity). LCMS: [M+H]+=256.21
Step 2: 6-(2-amino-6-fluoro-5-(6-methoxy-2-methyl-1,2,3,4-tetrahydroisoquinolin-7-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 7-bromo-6-methoxy-2-methyl-1,2,3,4-tetrahydroisoquinoline (98 mg, 0.191 mmol, 50% purity) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (95 mg, 0.249 mmol) to give the title compound as an off white solid (39 mg, 45% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.92 (d, J=7.95 Hz, 1H), 7.34-7.41 (m, 2H), 7.32 (s, 1H), 6.85 (s, 1H), 6.68-6.75 (m, 1H), 3.67 (s, 3H), 3.47 (s, 2H), 3.42-3.46 (m, 2H), 3.44 (t, J=6.66 Hz, 2H), 2.94 (t, J=6.60 Hz, 2H), 2.85-2.91 (m, 2H), 2.66 (t, J=5.99 Hz, 2H), 2.36 (s, 3 H); LCMS: [M+H]+=433.30.
Step 1: 7-bromo-6-fluoro-2-methylquinazolin-4(3H)-one
2-Amino-4-bromo-5-fluorobenzoic acid (1.0 g, 4.27 mmol) was taken up in acetic anhydride (5.65 ml, 59.8 mmol) and heated at 130° C. overnight. The reaction mixture was cooled to RT and left there for two hours. The precipitated solid was filtered, washed with cold ether, and dried under vacuum to give 2-acetamido-4-bromo-5-fluorobenzoic acid which was used in the next step without further purification. LCMS [M−H]− 273.89. The 2-acetamido-4-bromo-5-fluorobenzoic acid was take up in 27% aqueous ammonium hydroxide solution (3.33 ml, 23.07 mmol) and heated in a sealed tube at 80° C. for 4 hours. After cooling to RT, the solid was filtered, washed with water, and dried under vacuum to afford 7-bromo-6-fluoro-2-methylquinazolin-4(3H)-one (132 mg, 0.513 mmol, 12.02% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 12.42 (br. s., 1H), 7.96 (d, J=6.24 Hz, 1H), 7.87 (d, J=8.56 Hz, 1H), 2.35 (s, 3H); LCMS: [M+H]+ 257.09.
Step 2: (6-fluoro-2-methyl-4-oxo-3,4-dihydroquinazolin-7-yl)boronic acid
The product was prepared from 7-bromo-6-fluoro-2-methylquinazolin-4(3H)-one (86 mg, 0.335 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+ 289.31 Boronate, 207.18 Boronic acid.
Step 3: 7-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-6-fluoro-2-methylquinazolin-4(3H)-one
Prepared from (6-fluoro-2-methyl-4-oxo-3,4-dihydroquinazolin-7-yl)boronic acid (35.9 mg, 0.162 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (53 mg, 0.135 mmol) to give the title compound (47.2 mg, 71.4% yield) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 12.32 (br. s., 1H), 7.81 (d, J=9.41 Hz, 1H), 7.59-7.68 (m, 2H), 7.39 (d, J=7.95 Hz, 2H), 6.96 (d, J=8.80 Hz, 2H), 6.22 (s, 2H), 3.14 (d, J=4.65 Hz, 4H), 2.63-2.71 (m, 1H), 2.57 (br. s., 4H), 2.37 (s, 3H), 1.00 (d, J=6.48 Hz, 6H); LCMS: [M+H]+=491.36.
Step 1: Preparation of 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (150 mg, 0.424 mmol) and tert-butyl 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]tetrahydro-1(2H)-pyrazinecarboxylate (206 mg, 0.529 mmol). The title compound was isolated as a beige solid (205 mg, 97% yield). LCMS: [M+H]+=536.34
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared by deprotecting tert-butyl 4-(4-(6-amino-2-fluoro-5-(8-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate (205 mg, 0.371 mmol) with trifluoroacetic acid (2.56 mL, 33.4 mmol). The title compound was isolated as a beige solid (163 mg, quantitative yield). LCMS: [M+H]+=436.31
Step 3: 6-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.115 mmol) and 4-bromo-1,1-difluorobutane (59.6 mg, 0.344 mmol) to afford the product as an off-white solid (46 mg, 72%). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.98 (br. s., 1H), 7.58-7.69 (m, 1H), 7.38-7.47 (m, 2H), 7.30 (s, 1H), 7.26 (d, J=12.10 Hz, 1H), 6.92-7.04 (m, 2H), 6.28 (s, 2H), 5.99-6.25 (m, 1H), 3.35 (d, J=2.20 Hz, 2H), 3.17 (br. s., 4H), 2.94 (t, J=6.24 Hz, 2H), 2.52-2.56 (m, 4H), 2.35-2.42 (m, 2H), 1.77-1.96 (m, 2H), 1.59 (quin, J=7.37 Hz, 2H) LCMS: [M+H]+=528.37
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (500 mg, 1.412 mmol) and tert-butyl 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]tetrahydro-1(2H)-pyrazinecarboxylate (685 mg, 1.765 mmol) to give the title compound as a beige solid (638 mg, 84% yield). LCMS: [M+H]+=536.40
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one, Trifluoroacetic Acid, CF3COOH salt
Prepared from tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate (201 mg, 0.375 mmol) with Trifluoroacetic acid (2.59 ml, 33.8 mmol). The title compound was isolated as an off white solid (189 mg, 92%). LCMS: [M+H]+=436.31
Step 3: 6-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (55 mg, 0.100 mmol) and 4-bromo-1,1-difluorobutane (51.9 mg, 0.300 mmol) to give the title compound as a white solid (40 mg, 72% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.10 (br. s., 1H), 7.60 (dd, J=13.33, 10.27 Hz, 2H), 7.43 (d, J=7.09 Hz, 1H), 7.39 (d, J=8.19 Hz, 2H), 6.97 (d, J=8.68 Hz, 2H), 6.00-6.26 (m, 3H), 3.39-3.44 (m, 2H), 3.16 (d, J=4.52 Hz, 4H), 2.93 (t, J=6.30 Hz, 2H), 2.52-2.61 (m, 4H), 2.38 (t, J=6.97 Hz, 2H), 1.78-1.93 (m, 2H), 1.59 (quin, J=7.37 Hz, 2H); LCMS: [M+H]+=528.30.
Step 1: tert-butyl 4-(4-bromo-2-formylphenyl)piperazine-1-carboxylate
Prepared from 5-bromo-2-fluorobenzaldehyde (4 g, 19.70 mmol) and 1-Boc-piperazine (5.50 g, 29.6 mmol). The title compound was isolated as a pale-yellow oil (4.18 mg, 57% yield). LCMS: [M+H]+=369.31.
Step 2: tert-butyl 4-(4-bromo-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate
Prepared from tert-butyl 4-(4-bromo-2-formylphenyl)piperazine-1-carboxylate (0.495 g, 1.338 mmol) and dimethylamine, 2.0M solution in THF (5.35 ml, 10.70 mmol). The title compound was isolated as a pale-yellow oil (321 mg, 60% yield). LCMS: [M+H]+=398.30.
Step 3: Preparation of tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate
Prepared from tert-butyl 4-(4-bromo-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate (56 mg, 0.141 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (67.3 mg, 0.176 mmol) to give the title compound as an off white solid (45 mg, 53% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.02-8.10 (m, 1H), 7.71 (d, J=9.78 Hz, 1H), 7.59-7.64 (m, 1H), 7.53 (d, J=7.95 Hz, 1H), 7.46-7.51 (m, 2H), 7.22 (d, J=8.44 Hz, 1H), 3.66 (s, 2H), 3.61 (br. s., 4H), 3.52-3.57 (m, 2H), 3.05-3.12 (m, 2H), 2.88-2.96 (m, 4H), 2.30 (s, 6H), 1.49-1.56 (m, 9H); LCMS: [M+H]+=575.32.
Step 1: (S)-2-(3-bromophenyl)-1-methylpyrrolidine
Prepared from (2S)-2-(3-bromophenyl)pyrrolidine (60 mg, 0.265 mmol) and formaldehyde solution, 37% wt in water (0.059 mL, 0.796 mmol). The title compound was isolated as a colorless oil (54 mg, 85% yield). LCMS: [M+H]+=240.18
Step 2: (S)-6-(2-amino-6-fluoro-5-(3-(1-methylpyrrolidin-2-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from (S)-2-(3-bromophenyl)-1-methylpyrrolidine (27 mg, 0.112 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (53.9 mg, 0.141 mmol) to afford the title compound as a beige solid (26 mg, 50% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.92-7.97 (m, 1 H), 7.59 (d, J=9.78 Hz, 1H), 7.40-7.45 (m, 2H), 7.35-7.39 (m, 2H), 7.27-7.32 (m, 1H), 7.18-7.23 (m, 1H), 7.18-7.23 (m, 1H), 3.45 (t, J=6.66 Hz, 2H), 3.08-3.16 (m, 2H), 2.92-2.97 (m, 2H), 2.23-2.31 (m, 1H), 2.13-2.19 (m, 1H), 2.07-2.12 (m, 3H), 1.85-1.93 (m, 1H), 1.73-1.82 (m, 2H); LCMS: [M+H]+=416.88.
Prepared from (S)-2-(3-bromophenyl)-1-methylpyrrolidine (27 mg, 0.112 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (56.4 mg, 0.141 mmol) to afford the title compound as a beige solid 9 mg, 17.5% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.76 (d, J=10.03 Hz, 1H), 7.68-7.72 (m, 1H), 7.54 (s, 1H), 7.45-7.49 (m, 1H), 7.39-7.45 (m, 2 H), 7.32 (d, J=7.46 Hz, 1H), 3.56 (t, J=6.66 Hz, 2H), 3.16-3.24 (m, 2H), 3.04 (t, J=6.54 Hz, 2H), 2.38 (q, J=9.25 Hz, 1H), 2.25-2.32 (m, 1H), 2.22 (s, 3H), 1.96-2.05 (m, 1H), 1.85-1.92 (m, 2H); LCMS: [M+H]+=435.31
Step 1: Preparation of 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate (39 mg, 0.068 mmol) with Trifluoroacetic acid (0.520 ml, 6.79 mmol). The title compound was isolated as a beige solid (30 mg, 93% yield). LCMS: [M+H]+=475.34.
Step 2: 6-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)-3-((dimethylamino)methyl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (30 mg, 0.063 mmol) and 4-bromo-1, 1-difluorobutane (16.40 mg, 0.095 mmol) to give the title compound as a beige solid (20 mg, 52% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.06 (d, J=7.95 Hz, 1H), 7.71 (d, J=9.78 Hz, 1H), 7.60 (br. s., 1H), 7.53 (d, J=7.58 Hz, 1H), 7.44-7.50 (m, 2H), 7.18-7.28 (m, 1H), 5.83-6.10 (m, 1H), 3.63 (s, 2H), 3.57 (t, J=6.60 Hz, 2H), 3.08 (t, J=6.42 Hz, 2H), 3.00 (d, J=4.03 Hz, 4H), 2.68 (br. s., 4H), 2.48-2.56 (m, 2H), 2.23-2.34 (m, 6 H), 1.85-1.98 (m, 2H), 1.69-1.79 (m, 2H); LCMS: [M+H]+=567.23.
Prepared from 6-(2-amino-6-fluoro-5-(5-methyl-1,2,3,4-tetrahydroisoquinolin-7-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (39 mg, 0.097 mmol) and 4-bromo-1,1-difluorobutane (33.5 mg, 0.194 mmol) to afford the title compound as a beige solid (35 mg, 69%). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.05 (d, J=7.95 Hz, 1H), 7.63 (d, J=9.78 Hz, 1H), 7.52 (d, J=7.95 Hz, 1H), 7.45-7.49 (m, 1H), 7.21 (s, 1H), 7.11 (s, 1H), 5.82-6.11 (m, 1H), 5.82-6.11 (m, 1H), 3.70 (s, 2H), 3.57 (t, J=6.60 Hz, 2H), 3.08 (t, J=6.54 Hz, 2H), 2.80-2.90 (m, 4H), 2.62 (d, J=7.83 Hz, 2H), 2.26-2.32 (m, 3H), 1.86-1.98 (m, 2H), 1.74-1.85 (m, 2H). LCMS: [M+H]+=495.22.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate
Prepared from tert-butyl 4-(4-bromo-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate (58 mg, 0.146 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (73.0 mg, 0.182 mmol) to yield the title compound as a white solid (14 mg, 16% yield). LCMS: [M+H]+=593.30
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from tert-butyl 4-(4-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate (14 mg, 0.024 mmol) and TFA (0.181 mL, 2.362 mmol) to give product as a beige solid (11.5 mg, 99% yield). LCMS: [M+H]+=493.32
Step 3: 6-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)-3-((dimethylamino)methyl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (
Prepared from 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (11.5 mg, 0.023 mmol) and 4-bromo-1,1-difluorobutane (6.06 mg, 0.035 mmol) to give the title compound as an off white solid (11 mg, 77%). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.76 (d, J=9.90 Hz, 1H), 7.70 (d, J=9.78 Hz, 1H), 7.59 (s, 1H), 7.47 (d, J=8.31 Hz, 1H), 7.38-7.44 (m, 1 H), 7.24 (d, J=8.31 Hz, 1H), 5.80-6.13 (m, 1H), 3.61-3.69 (m, 2H), 3.56 (t, J=6.66 Hz, 2 H), 3.04 (t, J=6.60 Hz, 2H), 3.00 (t, J=4.46 Hz, 4H), 2.69 (br. s., 4H), 2.47-2.56 (m, 2H), 2.23-2.36 (m, 6H), 1.84-1.98 (m, 2H), 1.66-1.79 (m, 2H); LCMS: [M+H]+=585.41.
Prepared from 6-(2-amino-6-fluoro-5-(5-methyl-1,2,3,4-tetrahydroisoquinolin-7-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (39 mg, 0.097 mmol) and 2-bromoethyl methyl ether (26.9 mg, 0.194 mmol) to give the title compound as an off white solid (21 mg, 45%). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.02-8.08 (m, 1H), 7.58-7.64 (m, 1H), 7.48-7.53 (m, 1H), 7.45 (s, 1H), 7.17-7.22 (m, 1H), 7.04-7.10 (m, 1H), 3.71-3.76 (m, 2H), 3.65 (t, J=5.56 Hz, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.39 (s, 3H), 3.06 (t, J=6.66 Hz, 2H), 2.88-2.93 (m, 2H), 2.80-2.84 (m, 2H), 2.78 (t, J=5.62 Hz, 2H), 2.27 (s, 3H); LCMS: [M+H]+=461.32
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (38.9 mg, 0.110 mmol) and 2-cyclobutyl-7-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2,7-diazaspiro[3.5]nonane (38.2 mg, 0.10 mmol) to give the title compound (25 mg, 60% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.09 (br s, 1H), 7.59 (dd, J=10.1, 17.6 Hz, 2H), 7.42 (d, J=7.0 Hz, 1H), 7.36 (br d, J=8.3 Hz, 2H), 6.96 (d, J=8.8 Hz, 2H), 6.15 (s, 2H), 3.40 (br dd, J=4.0, 6.1 Hz, 2H), 3.17-3.05 (m, 5H), 2.91 (br s, 6H), 1.93-1.83 (m, 2H), 1.81-1.70 (m, 6H), 1.60 (s, 2H); LCMS: [M+H]+=530.44.
Step 1: 6-bromo-4,4-difluoro-3-methoxy-3-methyl-3,4-dihydroisoquinolin-1(2H)-one
A vial was charged with 6-bromo-3-methyl-2H-isoquinolin-1-one (300 mg, 1.260 mmol) and Selectfluor™ fluorinating reagent >95% in F+active (536 mg, 1.512 mmol). Methanol (6 ml) and acetonitrile (6 ml) were added, and the reaction was heated at 50° C. overnight. The mixture was purified by flash chromatography (Biotage, silica gel) eluting with 0-70% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum to give the product (81 mg, 21% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 9.43 (br. s., 1H), 7.91-7.95 (m, 2H), 7.86-7.89 (m, 1H), 3.13 (s, 3H), 1.54 (d, J=0.98 Hz, 3H); LCMS: [M+H]+=306.11.
Step 2: (4,4-difluoro-3-methoxy-3-methyl-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-4,4-difluoro-3-methoxy-3-methyl-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.131 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+=272.23 Boronic acid, 354.29 Boronate.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4,4-difluoro-3-methoxy-3-methyl-3,4-dihydroisoquinolin-1(2H)-one
Prepared from (4,4-difluoro-3-methoxy-3-methyl-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)boronic acid (34.7 mg, 0.128 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (42 mg, 0.107 mmol) to give the title compound (39.3 mg, 68.2% yield) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 9.32 (d, J=2.69 Hz, 1H), 8.01 (d, J=7.95 Hz, 1H), 7.83 (d, J=8.07 Hz, 1H), 7.81 (s, 1H), 7.64 (d, J=10.03 Hz, 1H), 7.42 (d, J=7.95 Hz, 2H), 6.96 (d, J=8.80 Hz, 2H), 6.25 (s, 2H), 3.15 (s, 3H), 3.12-3.15 (m, 4H), 2.68 (br. s., 1H), 2.58 (br. s., 4H), 1.56 (s, 3H), 1.01 (d, J=6.48 Hz, 6H); LCMS [M+H]+ 540.35.
Step 1: 5-bromo-2-(4-cyclopentylpiperazin-1-yl)benzaldehyde
Prepared from 5-bromo-2-fluorobenzaldehyde (0.8 g, 3.94 mmol) and 1-cyclopentylpiperazine (0.912 g, 5.91 mmol) to give the product as a pale-yellow solid (881 mg, 66% yield). LCMS: [M+H]+=337.26 Step 2: 1-(5-bromo-2-(4-cyclopentylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine
Prepared from 5-bromo-2-(4-cyclopentylpiperazin-1-yl)benzaldehyde (423 mg, 1.254 mmol) and dimethylamine, 2.0M solution in THF (5.02 ml, 10.03 mmol) as the amine to give the product was isolated as a pale-yellow solid (436 mg, 95% yield). LCMS: [M +H]+=366.36
Step 3: 6-(2-amino-5-(4-(4-cyclopentylpiperazin-1-yl)-3-((dimethylamino)methyl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(5-bromo-2-(4-cyclopentylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine (60 mg, 0.164 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (82 mg, 0.213 mmol) to yield the title compound as a beige solid (78 mg, 83% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.06 (d, J=7.95 Hz, 1H), 7.71 (d, J=9.78 Hz, 1H), 7.60 (s, 1H), 7.52-7.55 (m, 1H), 7.53 (dd, J=8.01, 1.28 Hz, 1H), 7.45-7.50 (m, 2H), 7.21-7.26 (m, 1H), 3.61-3.66 (m, 2H), 3.57 (t, J=6.66 Hz, 2H), 3.08 (t, J=6.60 Hz, 2H), 3.00 (t, J=4.58 Hz, 4 H), 2.67-2.88 (m, 4H), 2.58-2.65 (m, 1H), 2.29 (s, 6H), 1.98 (d, J=5.99 Hz, 2H), 1.73-1.81 (m, 2H), 1.61-1.68 (m, 2H), 1.45-1.53 (m, 2H); LCMS: [M+H]+=543.54
Prepared from 1-(5-bromo-2-(4-cyclopentylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine (60 mg, 0.164 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (85 mg, 0.213 mmol) to give the title compound as a white solid (36 mg, 37% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.62-7.67 (m, 1H), 7.58 (d, J=9.78 Hz, 1H), 7.47 (s, 1H), 7.35 (d, J=8.31 Hz, 1H), 7.30 (d, J=6.72 Hz, 1H), 7.12 (d, J=8.44 Hz, 1H), 3.53 (s, 2H), 3.44 (t, J=6.66 Hz, 2H), 2.92 (t, J=6.60 Hz, 2H), 2.88 (t, J=4.52 Hz, 4H), 2.55-2.76 (m, 4 H), 2.45-2.54 (m, 1H), 2.18 (s, 6H), 1.83-1.90 (m, 2H), 1.59-1.68 (m, 2H), 1.48-1.56 (m, 2H), 1.33-1.42 (m, 2H); LCMS: [M+H]+=561.53
Step 1: -butyl 4-(2-((dimethylamino)methyl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine-1-carboxylate
Prepared from tert-butyl 4-(4-bromo-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate (80 mg, 0.201 mmol) using similar procedures as described previously to afford the product as a black solid (89 mg, 97% yield) which was taken to the next step without any purification. LCMS: [M+H]+=446.40 Step 2: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2-dihydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate
Prepared from tert-butyl 4-(2-((dimethylamino)methyl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine-1-carboxylate (85 mg, 0.185 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)isoquinolin-1(2H)-one (49.5 mg, 0.148 mmol) to give the product as a brown solid (25 mg, 23% yield). LCMS: [M+H]+=573.44
Step 3: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)isoquinolin-1(2H)-one
Prepared from tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2-dihydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenyl)piperazine-1-carboxylate (25 mg, 0.034 mmol) with TFA (0.259 mL, 3.38 mmol) to give the title compound as a brown solid (12 mg, 75% yield); LCMS: [M+H]+=475.34.
Step 4: 6-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)-3-((dimethylamino)methyl)phenyl)-6-fluoropyridin-3-yl)isoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)isoquinolin-1(2H)-one (12 mg, 0.025 mmol) and 4-bromo-1,1-difluorobutane (8.79 mg, 0.051 mmol) to give the title compound as a beige solid (6 mg, 38% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.42 (d, J=8.31 Hz, 1H), 7.82 (s, 1H), 7.77 (d, J=9.66 Hz, 1H), 7.68 (d, J=8.31 Hz, 1H), 7.63 (s, 1H), 7.53-7.57 (m, 1H), 7.55 (d, J=8.31 Hz, 1H), 7.27-7.35 (m, 1H), 7.19-7.27 (m, 1H), 6.66-6.80 (m, 1H), 5.84-6.10 (m, 1H), 3.72-3.88 (m, 2H), 3.01 (t, J=4.46 Hz, 4H), 2.71 (br. s., 4H), 2.54 (d, J=7.34 Hz, 2H), 2.43 (s, 6H), 1.87-1.96 (m, 2H), 1.71-1.78 (m, 2H); LCMS: [M+H]+=565.54.
Step 1: 6-bromo-8-fluoro-3-methylisoquinolin-1(2H)-one
To an RBF was added copper(I) bromide (0.209 g, 1.455 mmol), cesium carbonate (9.48 g, 29.1 mmol), 2,4-dibromo-6-fluorobenzamide (4.32 g, 14.55 mmol), propan-2-one (5.34 ml, 72.7 mmol) and dimethylsulfoxide (DMSO) (150 ml). The reaction was stirred and heated at 80° C. overnight. The reaction mixture was partitioned between brine (200 ml) and DCM (200 ml). The organic layer was separated, and the aqueous layer washed with DCM (2×100 ml). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated onto celite. The mixture was purified by flash chromatography (Biotage, silica gel) eluting with 0-100% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum to afford the product (2.41 g, 64.7% yield) as a pale-yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.39 (br. s., 1H), 7.62 (s, 1H), 7.38 (d, J=11.00 Hz, 1H), 6.30 (s, 1H), 2.18 (s, 3H); LCMS: [M+H]+=256.08.
Step 2: (8-fluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-8-fluoro-3-methylisoquinolin-1(2H)-one (31 mg, 0.121 mmol) to give the boronic acid intermediate. LCMS: [M+H]+=222.35.
Step 3: 6-(2-amino-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from (8-fluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (31.6 mg, 0.143 mmol) and 3-chloro-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-2-amine (43 mg, 0.119 mmol) to give the title compound (16.2 mg, 27.1% yield) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.24 (s, 1H), 7.65 (d, J=10.15 Hz, 1H), 7.44 (s, 1H), 7.42 (d, J=8.19 Hz, 2H), 7.22 (d, J=12.47 Hz, 1H), 6.98 (d, J=8.44 Hz, 2H), 6.34 (s, 1H), 6.29 (br. s., 2H), 3.17 (br. s., 4H), 2.59 (br. s., 4H), 2.23 (br. s., 2H), 2.19 (s, 3H), 0.81-0.94 (m, 1H), 0.42-0.61 (m, 2H), 0.04-0.23 (m, 2H); LCMS: [M+H]+=502.37.
Step 1: 6-bromo-4-fluoroisoquinolin-1(2H)-one
A vial was charged with 6-bromo-2H-isoquinolin-1-one (1000 mg, 4.46 mmol) and Selectfluor™ (1897 mg, 5.36 mmol). Methanol (10 ml) and acetonitrile (10 ml) were added, and the reaction was heated to 50° C. for 1 h. The reaction was concentrated in vacuo, dissolved in 1,2-dichloroethane (20 ml), and phosphorous (V) oxychloride (0.835 ml, 8.93 mmol) was added. The reaction was stirred at 50° C. for 1 h. The mixture was poured into a RBF and concentrated in vacuo to remove the volatiles then the aqueous suspension was filtered, washing with water. The collected solid was dried under vacuum to give the product (1.1 g, quantitative) as a light pink solid. The material was used in the next step as-is. LCMS: [M+H]+=242.14.
Step 2: (4-fluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
The title compound was prepared from 6-bromo-4-fluoroisoquinolin-1(2H)-one (920 mg, 3.80 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+=290.31 Boronate, 208.21 Boronic acid.
Step 3: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (500 mg, 1.578 mmol) and (4-fluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (392 mg, 1.893 mmol) to give the product (544 mg, 88% yield) as a beige solid. LCMS [M+H]+=352.19.
Step 4: 1-(4-bromophenyl)-4-(cyclopropylmethyl)piperazine
To a solution of 1-(4-bromophenyl)piperazine (1781 mg, 7.39 mmol) and N,N-diisopropylethylamine (6.43 ml, 36.9 mmol) in N,N-dimethylformamide (10 ml) was added (bromomethyl)cyclopropane (1.433 ml, 14.77 mmol) then stirred at RT overnight. The mixture was diluted with water and extracted with DCM (3x). The combined organic layers were washed with brine, dried over anhydrous MgSO4 and concentrated onto celite. The crude material was purified by flash chromatography (Biotage, silica gel) eluting with 0-100% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum to afford the product (2038 mg, 93% yield) as an off-white solid. LCMS[M+H]+ 295.37.
Step 5: 1-(cyclopropylmethyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine
Prepared from 1-(4-bromophenyl)-4-(cyclopropylmethyl)piperazine (1.88 g, 6.37 mmol) to give the boronate which was used in the next step without further purification. LCMS: [M+H]+=343.58.
Step 6: 6-(2-amino-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (30 mg, 0.085 mmol) and 1-(cyclopropylmethyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (35.0 mg, 0.102 mmol) to give the title compound (10.1 mg, 24% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.15 (d, J=4.89 Hz, 1H), 8.27 (dd, J=8.31, 1.47 Hz, 1H), 7.82 (s, 1H), 7.73 (d, J=8.31 Hz, 1H), 7.67 (d, J=10.15 Hz, 1H), 7.43 (br. s., 1H), 7.41 (s, 2H), 6.98 (d, J=8.56 Hz, 2H), 6.28 (br. s., 2H), 3.17 (br. s., 4H), 2.58 (br. s., 4H), 2.23 (br. s., 2H), 0.87 (br. s., 1H), 0.49 (d, J=5.50 Hz, 2H), 0.11 (br. s., 2H); LCMS: [M+H]+ 488.39.
Step 1: 4-fluoro-3-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one
Prepared from 6-bromo-4-fluoro-3-methylisoquinolin-1(2H)-one (72 mg, 0.281 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+=222.23 Boronic acid, 304.29 Boronate.
Step 2: 3-chloro-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-2-amine
To a vial was added 5-bromo-3-chloro-6-fluoropyridin-2-amine (1200 mg, 5.32 mmol), 1-(cyclopropylmethyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (2186 mg, 6.39 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (389 mg, 0.532 mmol), Cs2CO3 (4336 mg, 13.31 mmol), 1,2-dimethoxyethane (12 mL) and water (3 mL) (4:1 DME/H2O). The vial was flushed with nitrogen, then heated at 90° C. for 7 hours. The reaction was concentrated onto celite and purified by flash chromatography (Biotage, silica gel) eluting with 0-10% MeOH/DCM+1% NH4OH, followed by flash chromatography eluting with 0-100% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum afford the product (1392 mg, 72.5% yield) as a beige solid. LCMS: [M+H]+=361.50.
Step 3: 6-(2-amino-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-4-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 4-fluoro-3-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one (40.7 mg, 0.134 mmol) and 3-chloro-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-2-amine (44 mg, 0.122 mmol) to give the title compound (15.8 mg, 25.8% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.25 (br. s., 1H), 8.18-8.24 (m, 1H), 7.75 (s, 1H), 7.62-7.68 (m, 2H), 7.42 (d, J=8.19 Hz, 2H), 6.99 (d, J=8.19 Hz, 2H), 6.26 (br. s., 2H), 3.17 (br. s., 4H), 2.62 (d, J=19.44 Hz, 2H), 2.24 (d, J=2.81 Hz, 3H), 0.88 (br. s., 1H), 0.50 (br. s., 2H), 0.12 (br. s., 2H); LCMS: [M+H]+=502.49.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (40 mg, 0.115 mmol) and 1-(cyclopropylmethyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (47.2 mg, 0.138 mmol) to give the title compound (11.4 mg, 20.5% yield) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.08 (d, J=5.26 Hz, 1H), 8.28 (d, J=8.31 Hz, 1H), 7.72 (s, 1H), 7.66 (d, J=10.15 Hz, 1H), 7.62 (d, J=8.19 Hz, 1H), 7.41 (d, J=8.19 Hz, 2H), 7.04 (d, J=5.26 Hz, 1H), 6.98 (d, J=8.68 Hz, 2H), 6.22 (s, 2H), 3.17 (br. s., 4H), 2.59 (br. s., 4H), 2.24 (s, 5H), 0.86 (d, J=5.62 Hz, 1H), 0.46-0.51 (m, 2H), 0.06-0.13 (m, 2H); LCMS [M+H]+ 484.32.
Step 1: tert-butyl 2-chloro-5-oxo-7,8-dihydropyrido[4,3-d]pyrimidine-6(5H)-carboxylate
To a solution of tert-butyl 2-chloro-7,8-dihydropyrido[4,3-d]pyrimidine-6(5H)-carboxylate (500 mg, 1.854 mmol), ruthenium(III) chloride hydrate 99.98% (125 mg, 0.556 mmol), sodium (meta)periodate (1189 mg, 5.56 mmol), CH2Cl2 (2 mL), and water (2 mL) was added 2 drops of acetonitrile. The reaction was stirred at RT overnight. The reaction was quenched with isopropanol (1 mL), filtered over celite and the filter cake was washed with DCM (20 mL). Water was added and the aqueous layer was extracted with CH2Cl2 (2×20 mL), and the combined organics were dried over anhydrous Na2SO4, then filtered and concentrated. The residue was purified by flash chromatography over silica gel (0-50% EtOAc/hexane eluent) to give the product (399 mg, 76% yield) as an off-white solid. LCMS: [M+H-tBu]+=228.18.
Step 2: tert-butyl2-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-5-oxo-7,8-dihydropyrido[4, 3-d]pyrimidine-6(5H)-carboxylate
Prepared from tert-butyl 2-chloro-5-oxo-7,8-dihydropyrido[4,3-d]pyrimidine-6(5H)-carboxylate (69.6 mg, 0.245 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (90 mg, 0.204 mmol) to give the product (50.2 mg, 44% yield) as a yellow solid. LCMS: [M+H]+=562.34.
Step 3: 2-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-7,8-dihydropyrido[4,3-d]pyrimidin-5(6H)-one, trifluoroacetate
Prepared from 2-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-5-oxo-7,8-dihydropyrido[4,3-d]pyrimidine-6(5H)-carboxylate (50.2 mg, 0.089 mmol) and TFA (0.137 mL, 1.788 mmol) to give the product (47.4 mg, 92% yield) as an orange solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 9.31 (br. s., 1H), 9.11 (s, 1H), 8.90 (d, J=10.52 Hz, 1H), 8.23 (br. s., 1H), 7.46 (d, J=8.07 Hz, 2H), 7.12 (d, J=8.80 Hz, 2H), 3.95 (d, J=13.20 Hz, 2H), 3.52-3.60 (m, 5H), 3.18-3.24 (m, 2H), 3.12-3.17 (m, 2H), 3.01 (t, J=12.23 Hz, 2H), 1.31 (d, J=6.60 Hz, 6H); LCMS: [M+H]+=462.32.
Prepared from 1-(5-bromo-2-methoxyphenyl)-N,N-dimethylmethanamine (50 mg, 0.205 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (102 mg, 0.266 mmol) afford the title compound as a peach colored solid (62 mg, 68% yield). 1H NMR ((500 MHz, METHANOL-d4) δ ppm 8.05 (d, J=7.95 Hz, 1H), 7.65-7.70 (m, 1H), 7.52 (dd, J=8.07, 0.98 Hz, 1H), 7.50 (s, 1H), 7.43-7.48 (m, 2H), 7.47 (d, J=5.87 Hz, 2H), 7.06 (d, J=8.56 Hz, 1H), 3.83-3.92 (m, 3H), 3.58-3.62 (m, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.07 (t, J=6.60 Hz, 2H), 2.30 (s, 6H); LCMS: [M+H]+=421.33.
Prepared from 1-(5-bromo-2-methoxyphenyl)-N,N-dimethylmethanamine (60 mg, 0.246 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (128 mg, 0.319 mmol) to afford the title compound as a white solid (36 mg, 32% yield). 1H NMR ((500 MHz, METHANOL-d4) δ ppm 7.76 (d, J=10.03 Hz, 1H), 7.66 (d, J=9.90 Hz, 1H), 7.48 (d, J=8.68 Hz, 1H), 7.45 (s, 1 H), 7.41-7.44 (m, 1H), 7.42 (d, J=6.85 Hz, 1H), 7.07 (d, J=8.44 Hz, 1H), 3.89 (s, 3H), 3.62 (s, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.04 (t, J=6.60 Hz, 2H), 2.31 (s, 6H); LCMS: [M+H]+=432.32.
Step 1: 5-bromo-2-(4-cyclobutylpiperazin-1-yl)benzaldehyde
Prepared from 5-bromo-2-fluorobenzaldehyde (0.45 g, 2.217 mmol) and 1-cyclobutylpiperazine (0.497 g, 3.55 mmol). The title compound was isolated as a pale-yellow solid (241 mg, 34% yield). LCMS: [M+H]+=323.29 Step 2: 1-(5-bromo-2-(4-cyclobutylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine
Prepared from 5-bromo-2-(4-cyclobutylpiperazin-1-yl)benzaldehyde (241 mg, 0.746 mmol) and dimethylamine, 2.0M solution in THF (1.491 ml, 2.98 mmol) as the amine to give the product as an off white solid (251 mg, 96% yield). LCMS: [M+H]+=352.31
Step 3: 6-(2-amino-5-(4-(4-cyclobutylpiperazin-1-yl)-3-((dimethylamino)methyl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(5-bromo-2-(4-cyclobutylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine (50 mg, 0.142 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (70.7 mg, 0.184 mmol) to yield the title compound as a peach colored solid (54 mg, 68% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.05 (d, J=7.95 Hz, 1H), 7.70 (d, J=9.66 Hz, 1H), 7.59 (s, 1H), 7.52 (d, J=7.95 Hz, 1H), 7.44-7.50 (m, 2H), 7.47 (br. s., 2H), 7.24 (d, J=8.31 Hz, 1H), 3.62 (s, 2H), 3.56 (t, J=6.60 Hz, 2H), 3.07 (t, J=6.48 Hz, 2H), 2.98 (br. s., 4H), 2.87-2.94 (m, 1H), 2.41-2.77 (m, 4H), 2.28 (s, 6H), 2.12 (d, J=2.81 Hz, 2H), 1.97 (quin, J=9.69 Hz, 2H), 1.75-1.85 (m, 2H); LCMS: [M+H]+=529.44.
Prepared from 1-(5-bromo-2-(4-cyclobutylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine (50 mg, 0.142 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (74.0 mg, 0.184 mmol to yield the title compound as a white solid (36 mg, 44% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.75 (d, J=10.03 Hz, 1H), 7.69 (d, J=9.78 Hz, 1H), 7.58 (s, 1 H), 7.45 (d, J=8.31 Hz, 1H), 7.41 (d, J=6.85 Hz, 1H), 7.23 (d, J=8.31 Hz, 1H), 3.61 (s, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.03 (t, J=6.54 Hz, 2H), 2.98 (t, J=4.40 Hz, 4H), 2.90 (t, J=7.83 Hz, 1H), 2.42-2.72 (m, 4H), 2.27 (s, 6H), 2.09-2.16 (m, 2H), 1.93-2.01 (m, 2H), 1.74-1.84 (m, 2H), 1.74-1.84 (m, 2H); LCMS: [M+H]+=547.43
Step 1: 5-bromo-2-methyl-1,2,3,4-tetrahydroisoquinoline
Prepared from 5-bromo-1,2,3,4-tetrahydroisoquinoline (750 mg, 3.54 mmol) and formaldehyde solution, 37% wt in water (0.790 ml, 10.61 mmol) to give the product as a colorless oil (794 mg, 99% yield). LCMS: [M+H]+=225.81
Step 2: 6-(2-amino-6-fluoro-5-(2-methyl-1,2,3,4-tetrahydroisoquinolin-5-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared as per the procedure analogous to that of Example 2, step 1, by coupling 5-bromo-2-methyl-1,2,3,4-tetrahydroisoquinoline (60 mg, 0.265 mmol) from Example 114 Step 1 with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (132 mg, 0.345 mmol) to afford the title compound as a peach colored solid (92 mg, 82% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.03 (d, J=7.95 Hz, 1H), 7.48 (d, J=7.83 Hz, 1H), 7.44 (s, 1H), 7.39 (d, J=9.41 Hz, 1H), 7.19-7.25 (m, 1H), 7.10 (t, J=6.97 Hz, 2H), 3.68 (br. s., 2H), 3.55 (t, J=6.54 Hz, 2H), 3.05 (t, J=6.42 Hz, 2H), 2.77 (br. s., 2H), 2.64-2.73 (m, 2H), 2.46 (s, 3H); LCMS: [M +H]+=403.40
Prepared from 5-bromo-2-methyl-1,2,3,4-tetrahydroisoquinoline (60 mg, 0.265 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (138 mg, 0.345 mmol) to afford the title compound as a white solid (49 mg, 42% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.74 (d, J=10.03 Hz, 1H), 7.35-7.43 (m, 2H), 7.19-7.25 (m, 1H), 7.10 (t, J=7.95 Hz, 2H), 3.68 (s, 2H), 3.54 (t, J=6.66 Hz, 2H), 3.02 (t, J=6.54 Hz, 2H), 2.77 (d, J=3.79 Hz, 2H), 2.73 (d, J=5.14 Hz, 2H), 2.46 (s, 3H); LCMS: [M+H]+=421.39
Prepared from 3-amino-7-chloro-2-methyl-3,4-dihydroquinazolin-4-one (30 mg, 0.143 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (76 mg, 0.172 mmol) to give the title compound (26.3 mg, 37.7% yield) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.15 (d, J=8.19 Hz, 1H), 7.70 (s, 1H), 7.64 (d, J=10.15 Hz, 1H), 7.60 (d, J=8.19 Hz, 1H), 7.42 (d, J=8.07 Hz, 2H), 6.96 (d, J=8.68 Hz, 2H), 6.21 (s, 2H), 5.85 (s, 2H), 3.12-3.17 (m, 4H), 2.63-2.70 (m, 1H), 2.61 (s, 3H), 2.56-2.59 (m, 4H), 1.00 (d, J=6.48 Hz, 6H); LCMS: [M+H]+=488.45.
Step 1: 6-bromo-4-chloroisoquinolin-1(2H)-one
To a solution of 6-bromo-2H-isoquinolin-1-one (600 mg, 2.68 mmol) in N,N-dimethylacetamide (14 ml) was added N-chlorosuccinimide (429 mg, 3.21 mmol). The mixture was stirred at 50° C. for 1 hour. The reaction was cooled to RT and diluted with water. The white suspension was filtered, washed with water and dried under vacuum to obtain 6-bromo-4-chloroisoquinolin-1(2H)-one (615 mg, 1.927 mmol, 72.0% yield, Purity (UV 254) 81%) as a white solid. The material was used in the next step without further purification. LCMS[M+H]+ 258.15.
Step 2: 4-chloro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one
Prepared from 6-bromo-4-chloroisoquinolin-1(2H)-one (132 mg, 0.511 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS[M+H]+ 306.24 Boronate, 224.17 Boronic acid.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4-chloroisoquinolin-1(2H)-one
Prepared from 4-chloro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one (74.6 mg, 0.110 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (36 mg, 0.092 mmol) to give the title compound (10.5 mg, 23.3% yield) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) b ppm 11.56 (br. s., 1H), 8.30 (d, J=8.31 Hz, 1H), 7.87 (s, 1H), 7.72 (d, J=8.19 Hz, 1H), 7.66 (d, J=10.03 Hz, 1H), 7.52 (d, J=5.14 Hz, 1H), 7.42 (d, J=7.70 Hz, 2H), 6.99 (d, J=6.36 Hz, 2H), 6.26 (br. s., 2H), 3.14 (br. s., 4H), 2.67-2.73 (m, 1H), 2.54-2.61 (m, 4H), 1.03 (br. s., 6H); LCMS: [M+H]+=492.21.
Step 1: 7-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (60 mg, 0.163 mmol) and 4-piperazinylphenylboronic acid, pinacol ester (61.2 mg, 0.212 mmol) to give the title compound as a beige solid (35 mg, 48% yield). LCMS: [M+H]+=449.34.
Step 2: 7-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one
Prepared as per the procedure analogous to that of Example 86, step 3, by heating the intermediate from Example 91, step 1, 7-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (35 mg, 0.078 mmol) with 4-bromo-1,1-difluorobutane (27.0 mg, 0.156 mmol). The title compound was isolated as a pale-yellow solid (17 mg, 38%). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.69 (d, J=9.66 Hz, 1H), 7.56 (s, 1H), 7.46 (d, J=8.19 Hz, 2H), 7.35 (d, J=11.49 Hz, 1H), 7.05 (d, J=8.68 Hz, 2 H), 5.83-6.09 (m, 1H), 3.27 (d, J=5.01 Hz, 4H), 2.66-2.71 (m, 4H), 2.48-2.52 (m, 2H), 2.46 (s, 3H), 1.85-1.96 (m, 2H), 1.71-1.78 (m, 2H); LCMS: [M+H]+=541.41
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2-methylquinazolin-4(3H)-one (40 mg, 0.115 mmol) and (1S,5R)-3-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (43.2 mg, 0.115 mmol) to give the title compound (43.6 mg, 86% yield). 1H NMR (500 MHz, DMSO-d6) δ=12.22 (s, 1H), 8.13 (d, J=8.2 Hz, 1H), 7.73-7.64 (m, 2H), 7.58 (br d, J=8.6 Hz, 1H), 7.53-7.43 (m, 2H), 7.19 (br d, J=7.8 Hz, 2H), 6.33 (s, 2H), 3.35-3.27 (m, 1H), 3.10-2.96 (m, 1H), 0.68-2.56 (m, 1H), 2.41-2.28 (m, 7H), 1.88-1.77 (m, 1H), 1.36 (t, J=4.0 Hz, 1H), 0.89-0.71 (m, 1H). LCMS: [M+H]+=443.39.
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (40 mg, 0.109 mmol) and (1S,5R)-3-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (41.1 mg, 0.109 mmol) to give the title compound (26.3 mg, 52.4% yield). 1H NMR (500 MHz, DMSO-d6) δ=12.25 (s, 1H), 7.69 (d, J=10.0 Hz, 1H), 7.53-7.44 (m, 3H), 7.33 (br d, J=11.2 Hz, 1H), 7.19 (br d, J=6.8 Hz, 2H), 6.42 (s, 2H), 3.32 (br dd, J=2.3, 4.3 Hz, 1H), 3.15-2.86 (m, 1H), 2.66-2.58 (m, 1H) 2.46-2.20 (m, 7H), 1.89-1.73 (m, 1H), 1.36 (t, J=4.0 Hz, 1H), 0.92-0.67 (m, 1H); [M+H]+=460.38;−111.13.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (40 mg, 0.114 mmol) reacted with (1 S,5R)-3-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (42.8 mg, 0.114 mmol), to give the title compound (14.7 mg, 28.9% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.15 (br s, 1H), 8.27 (br d, J=8.2 Hz, 1H), 7.82 (s, 1H), 7.72 (br t, J=9.8 Hz, 2H), 7.48 (br d, J=7.8 Hz, 2H), 7.41 (br s, 1H), 7.18 (br d, J=8.1 Hz, 2H), 6.38 (s, 2H), 3.39-3.29 (m, 1H), 3.01 (br d, J=1.7 Hz, 1H), 2.64-2.2.4 (m, 2H), 2.32 (br s, 3H), 1.81 (br s, 1H), 1.40-1.33 (m, 1H), 0.78 (br d, J=2.1 Hz, 1H). LCMS: [M+H]+=445.08.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (40 mg, 0.115 mmol) and (1S,5R)-3-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (43.3 mg, 0.115 mmol) to give the title compound (6.6 mg, 12.9% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.09 (br d, J=5.4 Hz, 1H), 8.29 (d, J=8.2 Hz, 1H), 7.72 (s, 1H), 7.68 (d, J=9.9 Hz, 1H), 7.62 (br d, J=8.3 Hz, 1H), 7.46 (br d, J=7.7 Hz, 2H), 7.19 (br d, J=8.1 Hz, 2H), 7.04 (s, 1H), 3.39-3.29 (m, 1H), 3.13-3.00 (m, 1H), 2.71-2.49 (m, 2H), 2.42-2.30 (m, 3H), 2.23 (s, 3H), 1.90-1.74 (m, 1H), 1.33 (t, J=4.2 Hz, 1H), 0.90-0.72 (m, 1H). LCMS: [M+H]+=441.38,
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2-methylquinazolin-4(3H)-one (50 mg, 0.115 mmol) and 1-cyclobutyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (39.2 mg, 0.115 mmol, in turn made from 4-piperazinylphenylboronic acid, pinacol ester and cyclobutanone in a manner similar to previous examples) to give the title compound (21.8 mg, 39.1% yield). 1H NMR (500 MHz, DMSO-d6) δ=12.20 (br s, 1H), 8.12 (d, J=8.2 Hz, 1H), 7.67 (s, 1H), 7.63 (d, J=10.1 Hz, 1H), 7.57 (br d, J=8.1 Hz, 1H), 7.45-7.37 (m, 2H), 6.96 (br d, J=8.8 Hz, 2H), 6.20 (s, 2H), 3.19-3.08 (m, 4H), 2.73 (quin, J=7.7 Hz, 1H), 2.42-2.32 (m, 7H), 2.05-1.93 (m, 2H), 1.86-1.75 (m, 2H), 1.71-1.58 (m, 2H). LCMS: [M+H]+=485.45.
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (40 mg, 0.109 mmol) and 1-cyclobutyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (37.3 mg, 0.109 mmol) to give the title compound (19.4 mg, 34.8% yield). 1H-NMR (500 MHz, DMSO-d6) δ=12.24 (br s, 1H), 7.66 (d, J=10.0 Hz, 1H), 7.50 (s, 1H), 7.43 (br d, J=8.3 Hz, 2H), 7.34 (br d, J=11.7 Hz, 1H), 6.97 (d, J=8.7 Hz, 2H), 6.31 (s, 2H), 3.23-3.09 (m, 4H), 2.74 (quin, J=7.6 Hz, 1H), 2.43-2.37 (m, 4H), 2.35 (s, 3H), 2.04-1.95 (m, 2H), 1.88-1.77 (m, 2H), 1.70-1.61 (m, 2H). LCMS: [M+H]+=503.37.
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2-methylquinazolin-4(3H)-one (50 mg, 0.115 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (39.7 mg, 0.115 mmol), to afford the title compound (21.5 mg, 37.8% yield). 1 H NMR (500 MHz, DMSO-d6) δ=12.21 (br s, 1H), 8.12 (d, J=8.2 Hz, 1H), 7.67 (s, 1H), 7.63 (d, J=10.1 Hz, 1H), 7.57 (br d, J=8.1 Hz, 1H), 7.53 (s, 1H), 7.43 (br d, J=8.4 Hz, 1H), 7.13 (d, J=8.3 Hz, 1H), 6.28 (s, 2H), 3.74 (br d, J=4.2 Hz, 4H), 3.45 (s, 2H), 2.94 (br s, 4H), 2.37 (s, 3H), 2.19 (s, 6H). LCMS: [M+H]+=489.46;
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (40 mg, 0.109 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (37.7 mg, 0.109 mmol) to give the title compound (14.8 mg, 26.6% yield). 1H NMR (500 MHz, DMSO-d6) δ=12.24 (br s, 1H), 7.67 (br d, J=10.0 Hz, 1H), 7.54 (br s, 1H), 7.49 (s, 1H), 7.45 (br d, J=7.1 Hz, 1H), 7.33 (brd, J=11.5 Hz, 1H), 7.14 (br d, J=7.3 Hz, 1H), 6.39 (br s, 2H), 3.75 (br s, 4H), 3.58-3.41 (m, 2H), 2.93 (br s, 4H), 2.34 (s, 3H), 2.21 (s, 6H); LCMS: [M+H]+=507.44.
Prepared from 1-(4-bromophenyl)-4-(3,3-difluoropropyl)piperazine (50 mg, 0.157 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (78 mg, 0.204 mmol) to give the title compound as a white solid (58 mg, 71% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.85-7.98 (m, 2H), 7.59 (d, J=10.15 Hz, 1H), 7.36-7.50 (m, 4H), 6.98 (d, J=8.56 Hz, 2H), 5.96-6.28 (m, 3H), 3.41 (d, J=3.91 Hz, 2H), 3.17 (br. s., 4H), 2.96 (t, J=6.30 Hz, 2H), 2.54 (d, J=4.28 Hz, 4H), 2.47 (br. s., 2H), 1.98-2.12 (m, 2H); LCMS: [M+H]+=496.41.
Step 1: 1-(4-bromophenyl)-4-(3,3-dimethoxypropyl)piperazine
To a solution of 1-(4-bromophenyl)piperazine (15 g, 54.034 mmol) in ACN (150 mL), 3-bromo-1,1-dimethoxypropane (14.83 g, 81.05 mmol) and K2CO3 (26.09 g, 189.12 mmol) were added at room temperature and the reaction mass was stirred at RT for 16 h. The reaction mixture was poured into water (400 mL) and extracted with EtOAc (3×400 mL). The combined organic was dried over Na2SO4 and concentrated under vacuum to afford the crude which was purified by column chromatography in neutral alumina and the product was eluted in neat DCM to afford the product (12 g, 56.2% yield) as a white solid. LCMS: [M+2]+=345.2.
Step 2: 1-(4-bromophenyl)-4-(3,3-difluoropropyl)piperazine
To a solution of 1-(4-bromophenyl)-4-(3,3-dimethoxypropyl)piperazine(12 g, 34.15 mmol) in THF (120 mL), 10% dil. HCl (10 V) was added dropwise at room temperature. After completion of addition, reaction was stirred at 80° C. for 30 min. After completion of the reaction, the reaction mass was poured into sat. NaHCO3 solution (400 mL) and extracted with DCM (2×100 mL). The combined organic layer was dried over anhydrous sodium sulfate filtered and cooled to −78° C., to this under nitrogen atmosphere, DAST (39.05 g, 242.26 mmol) was added dropwise. The reaction mixture was further stirred at same temperature for 3 h. After completion of reaction, reaction mass was poured into sat. NaHCO3 solution (400 mL) and extracted with DCM (3×100 mL). The combine organic layer was washed with water (2×300 mL), brine (2×300 mL), dried over Na2SO4 and concentrated. The resulting crude product was purified by column chromatography in neutral alumina and the product was eluted in 14% EtOAc in hexanes to afford the title compound (1.7 g, 13.2% yield) as yellow solid. LCMS: [M+2]=321.05.
Step 3: 6-(2-amino-5-(4-(4-(3,3-difluoropropyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-1)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(4-bromophenyl)-4-(3,3-difluoropropyl)piperazine (60 mg, 0.188 mmol) with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (98 mg, 0.244 mmol) to give the title compound as a white solid (82 mg, 76% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.10 (br. s., 1H), 7.60 (dd, J=13.14, 10.21 Hz, 2H), 7.43 (d, J=6.85 Hz, 1H), 7.39 (d, J=8.31 Hz, 2H), 6.98 (d, J=8.56 Hz, 2H), 6.38-6.38 (m, 1H), 6.00-6.26 (m, 3H), 3.39-3.44 (m, 2H), 3.17 (d, J=4.40 Hz, 4H), 2.89-2.96 (m, 2H), 2.54 (d, J=4.89 Hz, 4H), 2.47 (br. s., 2H), 1.98-2.12 (m, 2H); LCMS: [M+H]+=514.21
Step 1: 2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)benzaldehyde
2-Bromo-5-iodobenzaldehyde (7 g, 22.51 mmol), 3,6-dihydro-2H-pyran-4-boronic acid, pinacol ester (5.20 g, 24.77 mmol), tetrakis(triphenylphosphine)palladium(0) (1.301 g, 1.126 mmol) and K2CO3 (6.22 g, 6.43 mmol) were mixed together in 1,4-dioxane (70 mL) and water (14 mL) under vacuum. The reaction mixture was then stirred at 85° C. overnight. Additional tetrakis(triphenylphosphine)Palladium(0) (0.65 g, 0.563 mmol) was added and stirred at 85° C. for an additional 24 h. The mixture was filtered, rinsed with EtOAc. The filtrate was concentrated with silica gel and purified by column chromatography, eluting with 0-5% EtOAc in hexanes to give the product (4.13 g, 68.7% yield).
Step 2: Ethyl (E)-3-(2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)phenyl)acrylate
(Carbethoxymethylene)triphenyl phosphorane (7.00 g, 20.10 mmol) was mixed with 2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)benzaldehyde (4.13 g, 15.46 mmol) in DCM (80 mL) under ice-water bath and stirred at r.t. overnight The reaction mixture was concentrated with silica gel, purified by column chromatography, eluted with 0-10% EtOac in hexanes to give ethyl (E)-3-(2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)phenyl)acrylate 4.33 g. Yield=83%.
Step 3: Ethyl 3-(2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)phenyl)-4-nitrobutanoate
Ethyl (E)-3-(2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)phenyl)acrylate (4.30 g, 12.75 mmol) was mixed with nitromethane (7.78 g, 128 mmol) in acetonitrile (50 ml) under ice water bath, then 1,8-diazabicyclo [5.4.0]undec-7-ene (1.941 g, 12.75 mmol) was added. After being stirred at r.t. overnight, the reaction mixture was concentrated and the residue was diluted with EtOAc, concentrated onto silica gel, purified by column chromatography, eluted with 0-30% EtOAc in hexanes to give the product (3.897 g, 77% yield) as colorless sticky oil.
Step 4: 4-(2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)phenyl)pyrrolidin-2-one
Ethyl 3-(2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)phenyl)-4-nitrobutanoate (3.89 g, 9.77 mmol) was mixed with ammonium chloride (15.67 g, 293 mmol) in ethanol (75 ml) and water (35 ml) under 60° C. oil bath, then iron powder (5.45 g, 98 mmol) was added. The reaction mixture was stirred at 60° C. for 45 minutes. NaHCO3 (24.62 g, 293 mmol) was added in portions and the reaction mixture was stirred under 70° C. oil bath overnight. The mixture was filtered, rinsed with 95% EtOH. The filtrate was concentrated by Rotavapor to remove EtOH, extracted with EtOAc twice. The organic layer was dried over MgSO4, filtered, concentrated. The residue was triturated with ether to give the product (2.30 g, 73% yield) as off-white solid. LCMS: [M+H]+=322.22.
Step 5: 4-(5-(3,6-dihydro-2H-pyran-4-yl)-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidin-2-one
4-(2-bromo-5-(3,6-dihydro-2H-pyran-4-yl)phenyl)pyrrolidin-2-one (210 mg, 0.652 mmol), bis(pinacolato)diboron (199 mg, 0.782 mmol), [1,1′-bis(diphenylphosphino)ferrocene] dichloropalladium(II) (47.7 mg, 0.065 mmol) and potassium acetate (128 mg, 1.304 mmol) were mixed together in 1,4-dioxane (12 mL) under vacuum and stirred under 85° C. oil bath overnight. Additional [1,1′-bis(diphenylphosphino)ferrocene] dichloropalladium(II) (30 mg, 0.041 mmol) and bis(pinacolato)diboron (50 mg, 0.197 mmol) were added, and stirred under 90 oC oil bath overnight again. The reaction mixture was filtered and the filtrate was concentrated purified by column chromatography, eluted with 0-100% EtOAc in hexanes to give the product (63 mg, 26.2% yield). LCMS: [M+H]+=370.49
Step 6: 6-(2-amino-5-(4-(3,6-dihydro-2H-pyran-4-yl)-2-(5-oxopyrrolidin-3-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (55.2 mg, 0.156 mmol) and 4-(5-(3,6-dihydro-2H-pyran-4-yl)-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidin-2-one (57.6 mg, 0.156 mmol) to give the title compound (030 mg, 36.6% yield)1H NMR (500 MHz, METHANOL-d4) 5=7.74 (d, J=10.0 Hz, 1H), 7.54 (s, 1H), 7.40 (br dd, J=7.3, 13.5 Hz, 3H), 7.22 (d, J=7.9 Hz, 1H), 6.25 (br s, 1H), 4.31 (br d, J=2.6 Hz, 2H), 3.94 (t, J=5.4 Hz, 2H), 3.77-3.67 (m, 2H), 3.53 (t, J=6.6 Hz, 2H), 3.01 (br t, J=6.5 Hz, 2H), 2.79-2.29 (m, 5H); LCMS: [M+H]+=517.41.
6-(2-Amino-5-(4-(3,6-dihydro-2H-pyran-4-yl)-2-(5-oxopyrrolidin-3-yl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (15 mg, 0.029 mmol) was mixed with 10% Palladium on carbon wet (3.09 mg, 0.029 mmol) in methanol(5 ml) and stirred under hydrogen balloon for 30 mins. The reaction mixture was filtered, and the filtrate was concentrated by Rotavapor. The residue was triturated with MeOH-ether to give the title compound (6.2 mg, 40.1% yield) as off-white solid. 1H NMR (500 MHz, METHANOL-d4) δ=7.73 (br d, J=10.0 Hz, 1H), 7.47-7.33 (m, 3H), 7.28-7.13 (m, 2H), 4.05 (br d, J=10.1 Hz, 2H), 3.78-3.65 (m, 2H), 3.64-3.50 (m, 5H), 3.01 (br t, J=6.3 Hz, 2H), 2.94-2.80 (m, 1H), 2.78-2.31 (m, 2H), 1.90-1.73 (m, 4H), LCMS: [M+H]+=519.48;
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (45 mg, 0.128 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (53.1 mg, 0.153 mmol) to give the title compound (5.1 mg, 10.38 μmol, 8.1% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) b ppm 11.16 (br. s., 1H), 8.27 (d, J=8.19 Hz, 1H), 7.82 (s, 1H), 7.73 (d, J=8.31 Hz, 1H), 7.68 (d, J=10.15 Hz, 1H), 7.53 (br. s., 1H), 7.40-7.46 (m, 2H), 7.14 (d, J=8.31 Hz, 1H), 6.36 (s, 2H), 3.74 (br. s., 4H), 3.47 (br. s., 2H), 2.93 (br. s., 4H), 2.20 (br. s., 6H); LCMS: [M+H]+=492.53.
Step 1: 6-bromo-4,8-difluoro-3-methylisoquinolin-1(2H)-one
A vial was charged with 6-bromo-8-fluoro-3-methylisoquinolin-1(2H)-one (1.13 g, 4.41 mmol) and Selectfluor™ fluorinating reagent (1.641 g, 4.63 mmol). Methanol (10 mL) and acetonitrile (10 ml) were added, and the reaction was stirred at room temperature for 5 days. The white suspension was filtered, washed with water, and dried under vacuum to afford the product (1.01 g, 91% yield) as a white solid. LCMS: [M+H]+=274.14.
Step 2: (8-fluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-4,8-difluoro-3-methylisoquinolin-1(2H)-one (33 mg, 0.120 mmol) to give the boronic acid which was used in the next step without further purification. LCMS: [M+H]+=240.28.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4,8-difluoro-3-methylisoquinolin-1(2H)-one
Prepared from (4,8-difluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (25.3 mg, 0.106 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (22 mg, 0.056 mmol) to give the title compound (4.8 mg, 16.9% yield) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.24 (br. s., 1H), 7.68 (d, J=10.03 Hz, 1H), 7.53 (s, 1H), 7.37-7.45 (m, 3H), 6.97 (d, J=8.56 Hz, 2H), 6.35 (s, 2H), 3.15 (br. s., 4H), 2.67 (br. s., 1H), 2.58 (br. s., 4H), 2.22 (d, J=2.81 Hz, 3H), 1.01 (d, J=6.11 Hz, 6H); LCMS: [M+H]+=508.45.
Step 1: 6-bromo-4,8-difluoroisoquinolin-1(2H)-one
A vial was charged with 6-bromo-8-fluoro-1,2-dihydroisoquinolin-1-one (300 mg, 1.239 mmol) and Selectfluor™ fluorinating reagent (527 mg, 1.487 mmol). Methanol (3 mL) and acetonitrile (3 ml) were added, and the reaction was heated at 50° C. for 1 h. The reaction was concentrated in vacuo, dissolved in 1,2-dichloroethane (6 ml), and phosphorous (V) oxychloride (0.232 mL, 2.479 mmol) was added. The reaction was stirred at 50° C. for 1 h. The reaction was quenched into ice water and extracted with DCM (2x). The combined organic layers were dried with anhydrous sodium sulfate and concentrated to afford the product (306 mg, 95%) as a beige solid. The material was used in the next step without further purification. LCMS: [M+H]+=260.16.
Step 2: (4,8-difluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-4,8-difluoroisoquinolin-1(2H)-one (120 mg, 0.461 mmol) to give the boronic acid which was used in the next step without further purification. LCMS: [M+H]+=226.11.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4,8-difluoroisoquinolin-1(2H)-one
Prepared from (4,8-difluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (33.5 mg, 0.149 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (45 mg, 0.114 mmol) to give the title compound (20.2 mg, 35.8% yield) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.14 (br. s., 1H), 7.71 (d, J=10.15 Hz, 1H), 7.61 (s, 1H), 7.49 (d, J=12.59 Hz, 1H), 7.46 (d, J=5.50 Hz, 1H), 7.42 (d, J=8.31 Hz, 2H), 6.97 (d, J=8.68 Hz, 2H), 6.39 (s, 2H), 3.13-3.16 (m, 4H), 2.65-2.70 (m, 1H), 2.56-2.59 (m, 4H), 1.00 (d, J=6.36 Hz, 6H); LCMS: [M+H]+=494.28.
Prepared from (4,8-difluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (34.0 mg, 0.151 mmol) and 3-chloro-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-2-amine (42 mg, 0.116 mmol) to give the title compound (17.9 mg, 30.4% yield) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.14 (br. s., 1H), 7.71 (d, J=10.15 Hz, 1H), 7.61 (s, 1H), 7.49 (d, J=12.59 Hz, 1H), 7.46 (d, J=5.75 Hz, 1H), 7.42 (d, J=8.31 Hz, 2H), 6.98 (d, J=8.68 Hz, 2H), 6.39 (s, 2H), 3.14-3.20 (m, 4H), 2.55-2.60 (m, 4H), 2.22 (d, J=6.60 Hz, 2H), 0.81-0.90 (m, 1H), 0.46-0.50 (m, 2H), 0.10 (q, J=4.81 Hz, 2H); LCMS: [M+H]+=506.44.
Step 1: 7-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-2-methylquinazolin-4(3H)-one
Prepared from 7-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-2-methylquinazolin-4(3H)-one (45 mg, 0.105 mmol) and 4-piperazinylphenylboronic acid, pinacol ester (64.4 mg, 0.223 mmol) to give the product as a yellow solid (45 mg, 61%); LCMS: [M+H]+=431.36
Step 2: 7-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-2-methylquinazolin-4(3H)-one
Prepared from, 7-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-2-methylquinazolin-4(3H)-one (45 mg, 0.105 mmol) and 4-bromo-1,1-difluorobutane (36.2 mg, 0.209 mmol) to give the title compound as a pale-yellow solid (30 mg, 52% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.18 (d, J=8.19 Hz, 1H), 7.64 (s, 1H), 7.56 (dd, J=15.16, 9.05 Hz, 2H), 7.34 (d, J=8.31 Hz, 2H), 6.93 (d, J=8.68 Hz, 2H), 5.56-6.09 (m, 1 H), 3.16 (br. s., 4H), 2.57 (d, J=4.89 Hz, 4H), 2.35-2.42 (m, 5H), 1.72-1.84 (m, 2H), 1.62 (quin, J=7.55 Hz, 2H); LCMS: [M+H]+=523.46.
Step 1: 1-(3,3-difluoropropyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine
Prepared from 1-(4-bromophenyl)-4-(3,3-difluoropropyl)piperazine (225 mg, 0.705 mmol) and the resulting black solid was taken to the next step without any purification. (245 mg, 95% yield); LCMS: [M+H]+=367.42
Step 2: 7-(2-amino-5-(4-(4-(3,3-difluoropropyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one
Prepared from 1-(3,3-difluoropropyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (59.9 mg, 0.163 mmol) and 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (50 mg, 0.136 mmol) to yield the title compound as a pale-yellow solid (11.5 mg, 15% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.58 (d, J=9.66 Hz, 1H), 7.45 (s, 1H), 7.34 (d, J=8.19 Hz, 2H), 7.23 (d, J=11.49 Hz, 1H), 6.82-7.01 (m, 2H), 5.73-6.04 (m, 1H), 3.11-3.17 (m, 4H), 2.54-2.63 (m, 4H), 2.50 (t, J=7.58 Hz, 2H), 2.34 (s, 3H), 1.95-2.06 (m, 2H); LCMS: [M+H]+=527.50.
Step 1: N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine
Prepared from 1-(5-bromo-2-morpholinophenyl)-N,N-dimethylmethanamine (120 mg, 0.401 mmol) to afford the product as a brown solid which was used without further purification (139 mg, 90% yield); LCMS: [M+H]+=347.37
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-morpholinophenyl)-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (64 mg, 0.184 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (50 mg, 0.141 mmol) to afford the title compound as a beige solid (29 mg, 40% yield). 1H NMR (500 MHz, DMSO-d6) b ppm 8.00 (br. s., 1H), 7.63 (d, J=10.03 Hz, 1H), 7.52 (s, 1H), 7.43 (d, J=8.07 Hz, 1H), 7.30 (s, 1H), 7.26 (d, J=12.10 Hz, 1H), 7.14 (d, J=8.31 Hz, 1H), 6.38 (s, 2H), 3.75 (br. s., 4H), 3.45 (s, 2H), 3.25-3.32 (m, 2H), 2.94 (br. s., 6H), 2.19 (s, 6H); LCMS: [M+H]+=494.41.
Step 1: 5-bromo-2-thiomorpholinobenzaldehyde
Prepared as per the procedure analogous to that of Example 33, step 1, by the reaction between 5-Bromo-2-fluorobenzaldehyde (5 g, 24.63 mmol) and Thiomorpholine (3.56 g, 34.5 mmol). The title compound was isolated as a pale-yellow solid (3.171 g, 45% yield). LCMS: [M+H]+=286.11.
Step 2: 1-(5-bromo-2-thiomorpholinophenyl)-N,N-dimethylmethanamine
Prepared from 5-bromo-2-thiomorpholinobenzaldehyde (2.305 g, 8.05 mmol) and dimethylamine, 2.0M solution in THF (16.11 ml, 32.2 mmol) as the amine. The title compound was isolated as a beige solid (2.51 g, 99% yield). LCMS: [M+H]+=315.27
Step 3: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-thiomorpholinophenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(5-bromo-2-thiomorpholinophenyl)-N,N-dimethylmethanamine (50 mg, 0.159 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (79 mg, 0.206 mmol) to give the title compound as an off white solid (33 mg, 40% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.91 (d, J=7.82 Hz, 1H), 7.59 (d, J=10.03 Hz, 1H), 7.51 (s, 1H), 7.43-7.49 (m, 2H), 7.41 (d, J=8.07 Hz, 1H), 7.14 (d, J=8.31 Hz, 1H), 6.27 (s, 2 H), 3.39-3.47 (m, 4H), 3.16 (d, J=5.38 Hz, 4H), 2.95 (t, J=6.30 Hz, 2H), 2.77 (br. s., 4H), 2.19 (s, 6H); LCMS: [M+H]+=492.40
Prepared from 1-(5-bromo-2-thiomorpholinophenyl)-N,N-dimethylmethanamine (50 mg, 0.159 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (83 mg, 0.206 mmol) to give the title compound as a peach colored solid (39 mg, 46% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.11 (br. s., 1H), 7.60 (t, J=11.31 Hz, 2H), 7.49 (br. s., 1 H), 7.36-7.45 (m, 2H), 7.13 (d, J=8.19 Hz, 1H), 6.27 (br. s., 2H), 3.40-3.45 (m, 4H), 3.42 (br. s., 4H), 3.16 (br. s., 4H), 2.92 (t, J=6.05 Hz, 2H), 2.76 (br. s., 4H), 2.19 (s, 6H); LCMS: [M+H]+=510.39
Step 1: 6-bromo-4-chloro-8-fluoroisoquinolin-1(2H)-one
To a solution of 6-bromo-8-fluoro-1,2-dihydroisoquinolin-1-one (120 mg, 0.496 mmol) in N,N-dimethylacetamide (2 ml) was added N-chlorosuccinimide (79 mg, 0.595 mmol). The mixture was stirred at 50° C. for 1 hour then cooled to RT and diluted with water. The beige suspension was filtered, washed with water, and dried under vacuum to obtain the product (111 mg, 0.401 mmol, 81% yield) as a beige solid. The material was used at ˜80% purity (UV 254) in the next step without further purification. LCMS: [M+H]+=276.15.
Step 2: (4-chloro-8-fluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-4-chloro-8-fluoroisoquinolin-1(2H)-one (110 mg, 0.398 mmol) to give the boronic acid which was used in the next step without further purification. LCMS: [M+H]+=242.29.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4-chloro-8-fluoroisoquinolin-1(2H)-one
Prepared from (4-chloro-8-fluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (31.9 mg, 0.132 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (40 mg, 0.102 mmol) to give the title compound (5.3 mg, 10.2% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.56 (br. s., 1H), 7.66-7.72 (m, 2H), 7.55 (d, J=2.81 Hz, 1H), 7.49 (d, J=12.35 Hz, 1H), 7.41 (d, J=8.07 Hz, 2H), 6.97 (d, J=8.80 Hz, 2H), 6.37 (s, 2H), 3.15 (br. s., 4H), 2.67 (br. s., 1H), 2.58 (br. s., 4H), 1.01 (d, J=6.36 Hz, 6H); LCMS: [M+H]+=510.45.
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (40 mg, 0.109 mmol) and N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (37.6 mg, 0.109 mmol) to give the title compound (25.6 mg, 42.5% yield). 1H NMR (500 MHz, METHANOL-d4) δ=7.93 (d, J=9.7 Hz, 1H), 7.79 (s, 1H), 7.71 (br d, J=8.2 Hz, 1H), 7.67 (s, 1H), 7.62 (d, J=11.2 Hz, 1H), 7.56 (d, J=8.3 Hz, 1H), 4.52 (s, 2H), 4.06 (dd, J=3.7, 11.2 Hz, 2H), 3.68 (br t, J=11.2 Hz, 2H), 3.21-3.13 (m, 1H), 2.94 (s, 6H), 2.72 (s, 3H), 1.90 (dq, J=4.4, 12.5 Hz, 2H), 1.71 (br d, J=12.7 Hz, 2H); [M−HCl+H]+=506.50.
Step 1: 4-(4-bromo-2-(pyrrolidin-1-ylmethyl)phenyl)morpholine
Prepared from 5-bromo-2-morpholinobenzaldehyde (400 mg, 1.481 mmol) and pyrrolidine 99% (0.486 ml, 5.92 mmol) to afford the product as an off white solid (508 mg, 95% yield). LCMS: [M+H]+=325.28.
Step 2: 6-(2-amino-6-fluoro-5-(4-morpholino-3-(pyrrolidin-1-ylmethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 4-(4-bromo-2-(pyrrolidin-1-ylmethyl)phenyl)morpholine (60 mg, 0.166 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (83 mg, 0.216 mmol) to give the title compound as a beige solid (67 mg, 76% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.94 (br. s., 1H), 7.88-7.93 (m, 1H), 7.59 (d, J=10.15 Hz, 1H), 7.52 (s, 1H), 7.47 (d, J=8.07 Hz, 1H), 7.45 (s, 1H), 7.42-7.42 (m, 1H), 7.42-7.42 (m, 1H), 7.40 (d, J=8.19 Hz, 1H), 7.11 (d, J=8.31 Hz, 1H), 6.25 (s, 2H), 3.73-3.82 (m, 4H), 3.65 (s, 2H), 3.40-3.45 (m, 2H), 2.91-3.01 (m, 6H), 2.47-2.50 (m, 4H), 1.68 (br. s., 4H); LCMS: [M+H]+=502.62
Prepared from 4-(4-bromo-2-(pyrrolidin-1-ylmethyl)phenyl)morpholine (60 mg, 0.166 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (87 mg, 0.216 mmol) to yield the title compound as a white solid (38 mg, 42% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.11 (br. s., 1H), 7.60 (t, J=11.00 Hz, 2H), 7.51 (s, 1H), 7.43 (d, J=7.09 Hz, 1H), 7.38 (d, J=8.19 Hz, 1H), 7.11 (d, J=8.31 Hz, 1H), 6.26 (s, 2H), 3.72-3.79 (m, 4H), 3.65 (s, 2H), 3.40-3.43 (m, 2H), 2.91-3.00 (m, 6H), 2.53-2.62 (m, 4H), 1.68 (br.s., 4H); LCMS: [M+H]+=520.54.
Step 1: 4-(4-bromo-2-((dimethylamino)methyl)phenyl)thiomorpholine 1,1-dioxide
3-Chloroperbenzoic acid (159 mg, 0.923 mmol) was added to a solution of 1-(5-bromo-2-thiomorpholinophenyl)-N,N-dimethylmethanamine (150 mg, 0.462 mmol) in CH2Cl2 (10 mL), cooled in an ice bath, and the reaction mixture was continuously stirred at the same temperature for 30 min. The reaction mixture was quenched with water (10 mL) and then extracted with CHCl3/IPA (4:1) (3×10 mL). The combined organic layer was dried over sodium sulfate, filtered, concentrated and purified by silica gel chromatography, eluting with dichloromethane containing 0-10% MeOH and 0-1% NH4OH to isolate the title product as a beige solid (93 mg, 58% yield). LCMS: [M+H]+=347.24.
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(1,1-dioxidothiomorpholino)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 4-(4-bromo-2-((dimethylamino)methyl)phenyl)thiomorpholine 1,1-dioxide (46 mg, 0.132 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (66 mg, 0.172 mmol) yield the title compound as a beige solid (46 mg, 63% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.04 (d, J=7.95 Hz, 1H), 7.91 (s, 1H), 7.73 (dd, J=14.86, 9.23 Hz, 2H), 7.51 (dd, J=8.07, 5.14 Hz, 2H), 7.46 (s, 1H), 4.60 (s, 2H), 3.62-3.70 (m, 2H), 3.56 (t, J=6.60 Hz, 2 H), 3.26 (t, J=11.19 Hz, 2H), 3.18 (s, 6H), 3.02-3.12 (m, 6H); LCMS: [M+H]+=524.49
Step 1: 5-bromo-2-(4-cyclopropylpiperazin-1-yl)benzaldehyde
Prepared as per the procedure analogous to that of Example 33, step 1, by the reaction between 5-Bromo-2-fluorobenzaldehyde (2.5 g, 12.31 mmol) and 1-cyclopropyl-piperazine (1.554 g, 12.31 mmol). The title compound was isolated as a beige solid (3.595 mg, 80% yield, 85% purity). LCMS: [M+H]+=309.25
Step 2: 1-(5-bromo-2-(4-cyclopropylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine
Prepared using 5-bromo-2-(4-cyclopropylpiperazin-1-yl)benzaldehyde (500 mg, 1.374 mmol) and dimethylamine, 2.0M solution in THF (2.75 ml, 5.50 mmol) to give the product as a beige solid (477 mg, 97% yield). LCMS: [M+H]+=338.34
Step 3: 6-(2-amino-5-(4-(4-cyclopropylpiperazin-1-yl)-3-((dimethylamino)methyl)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(5-bromo-2-(4-cyclopropylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine (60 mg, 0.177 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (93 mg, 0.231 mmol) to yield the title compound as a beige solid (34.5 mg, 35% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.75 (d, J=10.03 Hz, 1H), 7.69 (d, J=9.54 Hz, 1H), 7.58 (s, 1 H), 7.43 (dd, J=14.24, 7.52 Hz, 2H), 7.21 (d, J=8.31 Hz, 1H), 3.61-3.67 (m, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.03 (t, J=6.42 Hz, 2H), 2.95 (br. s., 4H), 2.84 (br. s., 4H), 2.29 (s, 6H), 1.76-1.83 (m, 1H), 0.53-0.59 (m, 2H), 0.43-0.51 (m, 2H); LCMS: [M+H]+=533.58.
Prepared from 1-(5-bromo-2-(4-cyclopropylpiperazin-1-yl)phenyl)-N,N-dimethylmethanamine (50 mg, 0.148 mmol) from Example 99, step 2 with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (73.6 mg, 0.192 mmol) to give the title compound as a beige solid (59 mg, 74% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.01-8.08 (m, 1H), 7.66-7.73 (m, 1H), 7.58 (s, 1H), 7.50-7.54 (m, 1H), 7.42-7.48 (m, 2H), 7.17-7.24 (m, 1H), 3.61-3.66 (m, 2H), 3.56 (t, J=6.60 Hz, 2H), 3.06 (t, J=6.60 Hz, 2H), 2.93 (d, J=4.16 Hz, 4H), 2.83 (br. s., 4H), 2.25-2.33 (m, 6H), 1.78 (tt, J=6.71, 3.56 Hz, 1H), 0.52-0.58 (m, 2H), 0.44-0.51 (m, 2H); LCMS: [M+H]+=515.59
Prepared from 4-(4-bromo-2-(morpholinomethyl)phenyl)morpholine (50 mg, 0.147 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and morpholine analogous to previous procedures) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (73.0 mg, 0.190 mmol) to afford the title compound as a white solid (52 mg, 65% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.87-8.00 (m, 2H), 7.60 (d, J=10.03 Hz, 1H), 7.53 (s, 1H), 7.38-7.49 (m, 3H), 7.16 (d, J=8.31 Hz, 1H), 6.27 (s, 2H), 3.75 (br. s., 4H), 3.54 (br. s., 6H), 3.40 (d, J=4.16 Hz, 2H), 2.96 (br. s., 6H), 2.42 (br. s., 4H); LCMS: [M+H]+=518.654
Prepared from 4-(4-bromo-2-(pyrrolidin-1-ylmethyl)phenyl)morpholine (60 mg, 0.166 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (92 mg, 0.229 mmol) to give the title compound as a white solid (67 mg, 68% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.12 (br. s., 1H), 7.57-7.66 (m, 2H), 7.56-7.57 (m, 1H), 7.51 (s, 1H), 7.35-7.46 (m, 2H), 7.42 (dd, J=11.86, 7.83 Hz, 2H), 7.08-7.21 (m, 1H), 6.28 (s, 2H), 3.74 (d, J=4.16 Hz, 4H), 3.49-3.58 (m, 6H), 3.39-3.45 (m, 2H), 2.90-3.00 (m, 6H), 2.36-2.46 (m, 4H), 2.36-2.46 (m, 4H); LCMS: [M+H]+=536.46
Prepared from 4-(4-bromo-2-((dimethylamino)methyl)phenyl)thiomorpholine 1,1-dioxide (46 mg, 0.132 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (69 mg, 0.172 mmol) to give the title compound as an off white solid (34 mg, 45% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.17 (br. s., 1H), 7.93 (br. s., 1H), 7.56-7.66 (m, 3H), 7.34-7.47 (m, 2H), 6.33 (br. s., 2H), 4.35 (br. s., 2H), 3.43-3.51 (m, 4H), 3.14 (br. s., 2H), 2.99 (br. s., 6H), 2.92 (d, J=8.80 Hz, 6H), 1.39 (br. s., 1H); LCMS: [M+H]+=542.35
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (60 mg, and (2S,6R)-1,2,6-trimethyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (113 mg, 0.341 mmol to give the title compound as a grey solid (0.062 mmol, 36% yield). 1H NMR (500 MHz, DMSO-d6) δ 11.79-10.41 (m, 1H), 8.26 (dd, J=8.2, 1.7 Hz, 1H), 7.82 (d, J=0.9 Hz, 1H), 7.72 (dd, J=8.3, 1.3 Hz, 1H), 7.67 (d, J=10.1 Hz, 1H), 7.44-7.37 (m, 3H), 6.97 (d, J=8.8 Hz, 2H), 6.28 (s, 2H), 3.59 (br d, J=11.1 Hz, 2H), 2.40 (t, J=11.3 Hz, 2H), 2.28-2.20 (m, 2H), 2.18 (s, 3H), 1.07 (d, J=6.1 Hz, 6H); LCMS: [M+1]+=476.09.
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2-methylquinazolin-4(3H)-one (60 mg, 0.172 mmol) and (2S,6R)-1,2,6-trimethyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (114 mg, 0.344 mmol), 1H NMR (500 MHz, DMSO-d6) δ 12.64-11.87 (m, 1H), 8.12 (d, J=8.2 Hz, 1H), 7.66 (d, J=1.1 Hz, 1H), 7.62 (d, J=10.1 Hz, 1H), 7.57 (dd, J=8.1, 1.4 Hz, 1H), 7.40 (d, J=8.1 Hz, 2H), 6.97 (d, J=8.9 Hz, 2H), 6.21 (s, 2H), 3.59 (br d, J=11.1 Hz, 2H), 2.43-2.37 (m, 2H), 2.37 (s, 3H), 2.27-2.21 (m, 2H), 2.18 (s, 3H), 1.07 (d, J=6.1 Hz, 6H); LCMS: [M+1]+=473.46.
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-5-fluoro-2-methylquinazolin-4(3H)-one (60 mg, 0.163 mmol) and (2S,6R)-1,2,6-trimethyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (108 mg, 0.327 mmol), to give the title compound as a grey solid, (45 mg, 55.6% yield). 1H NMR (500 MHz, DMSO-d6) δ 12.59-11.82 (m, 1H), 7.65 (d, J=10.0 Hz, 1H), 7.48 (d, J=1.1 Hz, 1H), 7.41 (d, J=7.9 Hz, 2H), 7.33 (dd, J=11.7, 1.2 Hz, 1H), 6.97 (d, J=8.9 Hz, 2H), 6.31 (s, 2H), 3.59 (br d, J=11.0 Hz, 2H), 2.39 (t, J=11.3 Hz, 2H), 2.34 (s, 3H), 2.27-2.20 (m, 2H), 2.18 (s, 3H), 1.07 (d, J=6.1 Hz, 6H); LCMS: [M+1]+=491.44.
Step 1: N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine
Prepared from 1-(5-bromo-2-morpholinophenyl)-N,N-dimethylmethanamine (132 mg, 0.441 mmol) to give a mixture of the boronate and boronic acid which was used in the next step without further purification. LCMS [M+H]+ 347.30 Boronate, 265.36 Boronic acid.
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-morpholinophenyl)-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (30 mg, 0.086 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (35.8 mg, 0.103 mmol) to give the title compound (6.6 mg, 15.7% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.10 (br. s., 1H), 8.28 (d, J=8.19 Hz, 1H), 7.72 (s, 1H), 7.67 (d, J=10.15 Hz, 1H), 7.62 (d, J=8.19 Hz, 1H), 7.52 (s, 1H), 7.42 (d, J=8.44 Hz, 1H), 7.13 (d, J=8.31 Hz, 1H), 7.04 (s, 1H), 6.31 (s, 2H), 3.72-3.77 (m, 4H), 3.45 (s, 2H), 2.91-2.96 (m, 4H), 2.24 (s, 3H), 2.18 (s, 6H); LCMS: [M+H]+=488.45.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (40 mg, 0.114 mmol) and N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (47.1 mg, 0.136 mmol) to give the title compound (23.6 mg, 42.4% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.17 (br. s., 1H), 8.27 (dd, J=8.31, 1.83 Hz, 1H), 7.82 (d, J=1.22 Hz, 1H), 7.69-7.75 (m, 2H), 7.39-7.47 (m, 3H), 7.34 (d, J=8.07 Hz, 1H), 6.39 (s, 2H), 3.95 (dd, J=10.82, 3.48 Hz, 2H), 3.41-3.46 (m, 4H), 3.16-3.23 (m, 1H), 2.16 (s, 6H), 1.70 (qd, J=12.25, 4.10 Hz, 2H), 1.58-1.64 (m, 2H); LCMS: [M+H]+=491.46.
Step 1: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (450 mg, 1.420 mmol) and (8-fluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (408 mg, 1.846 mmol) to give the product (120 mg, 13.6% yield) as a white solid. The material contains de-brominated product, used as-is in the next step. LCMS: [M+H]+=366.23.
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(tetrahydro-2H-pyran-4-yl)phenyl)-6-fluoropyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one AMC-4019-0489-08
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one (35 mg, 0.057 mmol) and N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (40.1 mg, 0.116 mmol) to give the title compound (22.7 mg, 46.5% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.25 (s, 1H), 7.68 (d, J=10.15 Hz, 1H), 7.42-7.46 (m, 2H), 7.39 (s, 1H), 7.34 (d, J=8.19 Hz, 1H), 7.22 (d, J=12.47 Hz, 1H), 6.39 (s, 2H), 6.34 (s, 1H), 3.95 (dd, J=10.70, 3.61 Hz, 2H), 3.41-3.47 (m, 4H), 3.16-3.23 (m, 1H), 2.19 (s, 3H), 2.16 (s, 6H), 1.66-1.76 (m, 2H), 1.58-1.65 (m, 2H); LCMS: [M+H]+=505.50.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one (35 mg, 0.057 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (36.8 mg, 0.106 mmol) to give the title compound (19.1 mg, 39% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.25 (s, 1H), 7.64 (d, J=10.03 Hz, 1H), 7.52 (s, 1H), 7.40-7.46 (m, 2H), 7.19-7.25 (m, 1H), 7.13 (d, J=8.44 Hz, 1H), 6.37 (s, 2H), 6.34 (s, 1H), 3.72-3.78 (m, 4H), 3.44 (s, 2H), 2.90-2.97 (m, 4H), 2.19 (s, 3H), 2.18 (s, 6H); LCMS: [M+H]+=506.44.
Prepared from 1-(5-bromo-2-(cyclopropylmethoxy)phenyl)-N,N-dimethylmethanamine (50 mg, 0.150 mmol, in turn made from 5-bromo-2-(cyclopropylmethoxy)benzaldehyde and dimethylamine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (74.5 mg, 0.194 mmol) afford the title compound as a beige solid (67 mg, 92% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.02-8.09 (m, 1H), 7.67 (d, J=9.78 Hz, 1H), 7.52 (dd, J=8.01, 1.28 Hz, 1H), 7.42-7.50 (m, 3H), 7.01 (d, J=8.56 Hz, 1H), 3.91 (d, J=6.72 Hz, 2H), 3.60-3.67 (m, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.07 (t, J=6.66 Hz, 2H), 2.27-2.35 (m, 6H), 1.30-1.35 (m, 1H), 0.62-0.67 (m, 2H), 0.38-0.42 (m, 2H); LCMS: [M+H]+=461.44
Prepared from 1-(5-bromo-2-(cyclopropylmethoxy)phenyl)-N,N-dimethylmethanamine (50 mg, 0.150 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (78 mg, 0.194 mmol) to afford the title compound as a white solid (56 mg, 74% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.75 (d, J=10.03 Hz, 1H), 7.64 (d, J=9.54 Hz, 1H), 7.39-7.48 (m, 3H), 6.97-7.02 (m, 1H), 3.90 (d, J=6.72 Hz, 2H), 3.62 (d, J=2.93 Hz, 2H), 3.55 (t, J=6.66 Hz, 2H), 3.03 (t, J=6.05 Hz, 2H), 2.24-2.41 (m, 6H), 1.30-1.35 (m, 1H), 0.59-0.69 (m, 2H), 0.35-0.45 (m, 2H); LCMS: [M+H]+=479.37
Prepared from 1-(5-bromo-2-(cyclopropylmethoxy)benzyl)-4-methoxypiperidine (45 mg, 0.127 mmol, in turn made from 5-bromo-2-(cyclopropylmethoxy)benzaldehyde and 4-methoxypiperidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (63.3 mg, 0.165 mmol) to afford the title compound as a beige solid (36 mg, 51% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.99-8.09 (m, 1H), 7.60-7.69 (m, 1H), 7.47-7.53 (m, 2H), 7.45 (s, 1H), 7.41 (d, J=8.31 Hz, 1H), 6.98 (dd, J=8.56, 1.71 Hz, 1H), 3.89 (d, J=6.72 Hz, 2H), 3.66 (s, 2H), 3.56 (t, J=6.60 Hz, 2H), 3.33 (s, 3H), 3.23-3.30 (m, 1H), 3.06 (t, J=6.42 Hz, 2H), 2.85 (br. s., 2H), 2.34 (t, J=9.17 Hz, 2H), 1.92 (d, J=10.64 Hz, 2H), 1.54-1.67 (m, 2H), 1.29-1.34 (m, 1H), 0.60-0.68 (m, 2H), 0.36-0.42 (m, 2H); LCMS: [M+H]+=531.51
Prepared from 1-(5-bromo-2-(cyclopropylmethoxy)benzyl)-4-methoxypiperidine (45 mg, 0.127 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (66.2 mg, 0.165 mmol) afford the title compound as a white solid (30 mg, 41% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.75 (d, J=10.03 Hz, 1H), 7.64 (d, J=9.78 Hz, 1H), 7.48 (s, 1 H), 7.41 (d, J=7.09 Hz, 2H), 6.98 (d, J=8.56 Hz, 1H), 3.89 (d, J=6.72 Hz, 2H), 3.64-3.68 (m, 2H), 3.55 (t, J=6.66 Hz, 2H), 3.33 (s, 3H), 3.23-3.30 (m, 1H), 3.03 (t, J=6.48 Hz, 2H), 2.84 (br. s., 2H), 2.33 (t, J=9.60 Hz, 2H), 1.92 (d, J=10.88 Hz, 2H), 1.54-1.66 (m, 2H), 1.29-1.34 (m, 1H), 0.62-0.69 (m, 2H), 0.38 (q, J=4.93 Hz, 2H); LCMS: [M+H]+=549.56.
Prepared from 4-(4-bromo-2-((4-methoxypiperidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.162 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and 4-methoxypiperidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (81 mg, 0.211 mmol) give the title compound as an off white solid (58 mg, 62% yield). 1 H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.60 (d, J=10.15 Hz, 1H), 7.52 (s, 1H), 7.47 (d, J=7.95 Hz, 1H), 7.45 (s, 1H), 7.41 (d, J=8.44 Hz, 1H), 7.14 (d, J=8.31 Hz, 1H), 6.26 (s, 2H), 3.73-3.79 (m, 4H), 3.51 (s, 2H), 3.40-3.44 (m, 2H), 3.21 (s, 3H), 3.17 (d, J=5.14 Hz, 1H), 2.93-2.99 (m, 6H), 2.67-2.75 (m, 2H), 2.14 (t, J=9.66 Hz, 2H), 1.80 (d, J=9.90 Hz, 2H), 1.32-1.43 (m, 2H); LCMS: [M+H]+=546.55.
Prepared from 4-(4-bromo-2-((4-methoxypiperidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.162 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (85 mg, 0.211 mmol) to yield the title compound as a white solid (42 mg, 44% yield). 1H NMR (500 MHz, CHLOROFORM-d) δ ppm 7.90 (d, J=9.90 Hz, 1H), 7.60 (d, J=9.54 Hz, 1 H), 7.57 (s, 1H), 7.42 (d, J=8.19 Hz, 1H), 7.30 (d, J=6.60 Hz, 1H), 7.13 (d, J=8.31 Hz, 1H), 6.57 (br. s., 1H), 3.82-3.87 (m, 4H), 3.63 (td, J=6.57, 2.75 Hz, 2H), 3.57 (s, 2H), 3.32-3.36 (m, 3H), 3.20-3.26 (m, 1H), 3.03 (t, J=4.46 Hz, 6H), 2.76-2.85 (m, 2H), 2.20 (t, J=9.72 Hz, 2H), 1.84-1.92 (m, 2H), 1.50-1.58 (m, 2H); LCMS: [M+H]+=564.54.
Prepared from 4-(4-bromo-2-((4-methoxypiperidin-1-yl)methyl)phenyl)thiomorpholine (58 mg, 0.151 mmol, in turn made using 5-bromo-2-thiomorpholinobenzaldehyde and 4-methoxypiperidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (75 mg, 0.196 mmol) to give the title compound as a white solid (68 mg, 76% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.60 (d, J=10.15 Hz, 1H), 7.50 (s, 1H), 7.46-7.49 (m, 1H), 7.45 (s, 1H), 7.41 (d, J=8.31 Hz, 1H), 7.14 (d, J=8.31 Hz, 1H), 6.27 (s, 2H), 3.47 (s, 2H), 3.40-3.43 (m, 2H), 3.20-3.22 (m, 3H), 3.17 (t, J=5.50 Hz, 5H), 2.95 (t, J=6.54 Hz, 2H), 2.73-2.78 (m, 4H), 2.67-2.73 (m, 2H), 2.14 (t, J=9.78 Hz, 2 H), 1.81 (d, J=10.03 Hz, 2H), 1.37 (d, J=9.41 Hz, 2H); LCMS: [M+H]+=562.53
Prepared from 4-(4-bromo-2-((4-methoxypiperidin-1-yl)methyl)phenyl)thiomorpholine (58 mg, 0.151 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (79 mg, 0.196 mmol) to yield the title compound as a white solid (36 mg, 35% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.11 (br. s., 1H), 7.55-7.65 (m, 2H), 7.48 (s, 1H), 7.43 (d, J=7.09 Hz, 1H), 7.40-7.40 (m, 1H), 7.39 (d, J=8.31 Hz, 1H), 7.08-7.20 (m, 1H), 6.14-6.38 (m, 2H), 3.47 (s, 2H), 3.40-3.43 (m, 2H), 3.21 (s, 3H), 3.17 (br. s., 5H), 2.92 (t, J=6.42 Hz, 2H), 2.73-2.79 (m, 4H), 2.68-2.73 (m, 2H), 2.13 (t, J=9.54 Hz, 2H), 1.80 (d, J=9.54 Hz, 2H), 1.37 (d, J=9.29 Hz, 2H); LCMS: [M+H]+=580.58.
Prepared from 5-(3,6-dihydro-2H-pyran-4-yl)-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzaldehyde (767 mg, 2.441 mmol) reacted with 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (908 mg, 2.56 mmol) to give the title compound (960 mg, 85% yield). 1H NMR (500 MHz, DMSO-d6) δ=9.98 (d, J=2.8 Hz, 1H), 8.12 (br s, 1H), 7.93 (d, J=2.0 Hz, 1H), 7.83 (dd, J=2.1, 8.2 Hz, 1H), 7.61 (dd, J=10.0, 13.3 Hz, 2H), 7.54-7.48 (m, 2H), 7.47-7.43 (m, 2H), 7.42-7.36 (m, 1H), 6.49 (s, 2H), 6.45 (br s, 1H), 4.27 (br d, J=2.7 Hz, 2H), 3.86 (t, J=5.4 Hz, 2H), 3.46-3.38 (m, 2H), 3.31 (br, s, 2H), 2.92 (br t, J=6.5 Hz, 2H); LCMS: [M+H]+=462.44.
Step 1: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (430 mg, 1.357 mmol) and 4-fluoro-3-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1 (2H)-one (494 mg, 1.628 mmol) to give the product (443 mg, 89% yield) as a beige solid. LCMS: [M+H]+=366.23.
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-morpholinophenyl)-6-fluoropyridin-3-yl)-4-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoro-3-methylisoquinolin-1(2H)-one (35 mg, 0.096 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (36.4 mg, 0.105 mmol) to give the title compound (9.4 mg, 19.5% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.26 (br. s., 1H), 8.22 (dd, J=8.19, 1.59 Hz, 1H), 7.74 (s, 1H), 7.58-7.69 (m, 4 H), 7.54 (br. s., 2H), 7.44 (d, J=6.24 Hz, 1H), 7.14 (d, J=8.07 Hz, 1H), 6.35 (br. s., 2H), 3.75 (d, J=3.67 Hz, 4H), 3.47 (br. s., 2H), 2.93 (br. s., 4H), 2.24 (d, J=2.81 Hz, 3H), 2.20 (br. s., 3H); LCMS: [M+H]+=506.44.
Prepared from 1-(5-bromo-2-methoxybenzyl)-3-methoxyazetidine (60 mg, 0.185 mmol, in turn made using 5-bromo-2-methoxybenzaldehyde and 3-methoxyazetidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (92 mg, 0.240 mmol) to afford the title compound as an off white solid (53.5 mg, 60% yield). 1H NMR ((500 MHz, METHANOL-d4) ppm 8.06 (d, J=7.95 Hz, 1H), 7.66 (d, J=9.78 Hz, 1H), 7.53 (dd, J=7.95, 1.59 Hz, 1H), 7.44-7.49 (m, 2H), 7.44-7.49 (m, 2H), 7.42 (s, 1H), 7.02-7.09 (m, 1H), 4.01-4.09 (m, 1H), 3.87-3.92 (m, 3H), 3.74 (s, 2H), 3.61-3.67 (m, 2H), 3.54-3.60 (m, 2H), 3.22-3.29 (m, 3H), 3.22-3.29 (m, 3H), 3.10-3.15 (m, 2H), 3.08 (t, J=6.66 Hz, 2H); LCMS: [M+H]+=463.51.
Prepared from 1-(5-bromo-2-methoxybenzyl)-3-methoxyazetidine (60 mg, 0.185 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (96 mg, 0.240 mmol) to afford the title compound as a white solid (36.5 mg, 39% yield). 1H NMR ((500 MHz, METHANOL-d4) b ppm 7.71-7.79 (m, 1H), 7.60-7.66 (m, 1H), 7.36-7.45 (m, 3H), 6.98-7.07 (m, 1H), 3.99-4.07 (m, 1H), 3.84-3.90 (m, 3H), 3.72 (s, 2H), 3.59-3.64 (m, 2H), 3.52-3.58 (m, 2H), 3.21-3.27 (m, 3H), 3.07-3.12 (m, 2H), 2.99-3.06 (m, 2H); LCMS: [M+H]+=481.50.
Prepared from 1-(5-bromo-2-methoxybenzyl)-4-methoxypiperidine (60 mg, 0.179 mmol, in turn made from 5-bromo-2-methoxybenzaldehyde and 4-methoxypiperidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (89 mg, 0.233 mmol) to afford the title compound as an off white solid (66 mg, 71% yield). 1H NMR ((500 MHz, METHANOL-d4) δ ppm 7.96-8.08 (m, 1H), 7.59-7.66 (m, 1H), 7.47-7.51 (m, 1H), 7.46 (s, 1H), 7.40-7.45 (m, 2H), 7.01 (d, J=8.56 Hz, 1H), 3.82-3.89 (m, 3H), 3.58 (s, 2H), 3.54 (t, J=6.66 Hz, 2H), 3.31 (s, 3H), 3.25 (dt, J=8.16, 4.29 Hz, 1H), 3.04 (t, J=6.66 Hz, 2H), 2.71-2.88 (m, 2H), 2.19-2.36 (m, 2H), 1.84-1.94 (m, 2H), 1.52-1.62 (m, 2H); LCMS: [M+H]+=491.52.
Prepared from 1-(5-bromo-2-methoxybenzyl)-4-methoxypiperidine (60 mg, 0.179 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (94 mg, 0.233 mmol) to afford the title compound as a white solid (36 mg, 37.5% yield). 1H NMR ((500 MHz, METHANOL-d4) b ppm 7.76 (d, J=10.03 Hz, 1H), 7.66 (d, J=9.78 Hz, 1H), 7.45-7.51 (m, 2H), 7.43 (d, J=6.85 Hz, 1H), 7.05 (d, J=8.44 Hz, 1H), 3.87-3.93 (m, 3H), 3.61-3.66 (m, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.32-3.32 (m, 3H), 3.32 (br. s., 3H), 3.28 (td, J=7.86, 3.36 Hz, 1H), 3.04 (t, J=6.66 Hz, 2H), 2.82 (br. s., 2H), 2.31 (br. s., 2H), 1.88-1.97 (m, 2H), 1.56-1.65 (m, 2H); LCMS: [M+H]+=509.51.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (40 mg, 0.114 mmol) and (1S,5R)-3-cyclobutyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-3-azabicyclo[3.1.0]hexane (38.5 mg, 0.114 mmol) to give the title compound (9.70 mg, 17.3% yield). 1H NMR (500 MHz, METHANOL-d4) 5=8.42 (dd, J=1.5, 8.4 Hz, 1H), 7.95 (d, J=1.2 Hz, 1H), 7.77 (dd, J=1.6, 8.3 Hz, 1H), 7.71 (d, J=9.8 Hz, 1H), 7.48 (br d, J=7.2 Hz, 2H), 7.27 (d, J=5.5 Hz, 1H), 7.24 (d, J=8.3 Hz, 2H), 3.37 (br d, J=9.3 Hz, 1H), 3.25-3.16 (m, 1H), 3.12 (br d, J=9.2 Hz, 1H), 2.80-2.65 (m, 2H), 2.13-1.96 (m, 4H), 1.86 (td, J=3.8, 7.9 Hz, 1H), 1.82-1.69 (m, 2H), 1.40 (t, J=4.5 Hz, 1H), 0.91 (br dd, J=4.7, 7.8 Hz, 1H); LCMS: [M+H]+=485.45.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoro-3-methylisoquinolin-1(2H)-one (40 mg, 0.109 mmol) and N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (45.3 mg, 0.131 mmol) to give the title compound (9.4 mg, 17.1% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.26 (s, 1H), 8.22 (dd, J=8.25, 1.77 Hz, 1H), 7.75 (d, J=1.22 Hz, 1 H), 7.69 (d, J=10.03 Hz, 1H), 7.64 (dd, J=8.25, 1.53 Hz, 1H), 7.45 (d, J=7.82 Hz, 1H), 7.40 (br. s., 1H), 7.34 (d, J=8.19 Hz, 1H), 6.37 (s, 2H), 3.95 (dd, J=10.64, 3.55 Hz, 2H), 3.40-3.48 (m, 4H), 3.16-3.25 (m, 1H), 2.24 (d, J=3.06 Hz, 3H), 2.16 (br. s., 6H), 1.66-1.76 (m, 2H), 1.58-1.65 (m, 2H); LCMS [M+H]+=505.44.
Prepared from 4-(4-bromo-2-((3-methoxyazetidin-1-yl)methyl)phenyl)morpholine (55 mg, 0.153 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and 3-methoxyazetidine) from and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (76 mg, 0.199 mmol) to give the title compound as an off white solid (62 mg, 74%). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.59 (d, J=10.15 Hz, 1H), 7.43-7.51 (m, 3H), 7.39 (d, J=8.31 Hz, 1H), 7.11 (d, J=8.31 Hz, 1H), 6.21-6.35 (m, 2H), 3.97 (quin, J=5.69 Hz, 1H), 3.72-3.80 (m, 4H), 3.66 (s, 2H), 3.50 (t, J=6.72 Hz, 2H), 3.40-3.43 (m, 2H), 3.14 (s, 3H), 2.96 (t, J=6.42 Hz, 2H), 2.86-2.93 (m, 6H); LCMS: [M+H]+=518.54.
Prepared from 4-(4-bromo-2-((3-methoxyazetidin-1-yl)methyl)phenyl)morpholine (55 mg, 0.153 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.199 mmol) to yield the title compound as a white solid (25 mg, 29% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.12 (br. s., 1H), 7.61 (dd, J=12.23, 10.27 Hz, 2H), 7.47 (s, 1H), 7.43 (d, J=7.09 Hz, 1H), 7.33-7.40 (m, 1H), 7.37 (d, J=8.31 Hz, 1H), 7.06-7.15 (m, 1H), 6.27 (s, 2H), 3.96 (quin, J=5.69 Hz, 1H), 3.71-3.80 (m, 4H), 3.63-3.70 (m, 2H), 3.48-3.55 (m, 2H), 3.40-3.44 (m, 2H), 3.10-3.16 (m, 3H), 2.86-2.98 (m, 8H), 2.86-2.98 (m, 8H); LCMS: [M+H]+=536.59.
Step 1: 5-bromo-2-(4,4-difluorobutoxy)benzaldehyde
A mixture of 5-bromosalicylaldehyde (0.2 g, 0.995 mmol), 4-bromo-1,1-difluorobutane (0.172 g, 0.995 mmol) and K2CO3 (0.275 g, 1.990 mmol) in DMF (4 ml) was stirred overnight at RT. The reaction mixture was partitioned between ethyl acetate (15 mL) and water (50 mL). The organic phase was separated, the aqueous layer was extracted with ethyl acetate (2×15 mL), the combined organic layers were washed with brine (2×50 mL), dried over Na2SO4 and evaporated in vacuo to get the desired product as a white solid (301 mg, 98%); LCMS: [M+H]+=293.14.
Step 2: 1-(5-bromo-2-(4,4-difluorobutoxy)phenyl)-N,N-dimethylmethanamine
Prepared from 5-bromo-2-(4,4-difluorobutoxy)benzaldehyde (160 mg, 0.519 mmol) and dimethylamine to give the product (110 mg, 62.5% yield). LCMS: [M+H]+=322.29
Step 3: 6-(2-amino-5-(4-(4,4-difluorobutoxy)-3-((dimethylamino)methyl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(5-bromo-2-(4,4-difluorobutoxy)phenyl)-N,N-dimethylmethanamine (55 mg, 0.162 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (81 mg, 0.211 mmol) to afford the title compound as a beige solid (51 mg, 60% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.05 (d, J=7.95 Hz, 1H), 7.64-7.68 (m, 1H), 7.51 (dd, J=8.01, 1.65 Hz, 1H), 7.44-7.49 (m, 3H), 7.04 (d, J=8.19 Hz, 1H), 5.90-6.16 (m, 1H), 4.10 (t, J=5.99 Hz, 2H), 3.54-3.60 (m, 4H), 3.06 (t, J=6.66 Hz, 2H), 2.29-2.35 (m, 6H), 1.99-2.13 (m, 4H); LCMS: [M+H]+=499.55.
Prepared from 1-(5-bromo-2-(4,4-difluorobutoxy)phenyl)-N,N-dimethylmethanamine (55 mg, 0.162 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (85 mg, 0.211 mmol) to afford the title compound as a beige solid (40 mg, 45% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.74 (d, J=10.03 Hz, 1H), 7.64-7.68 (m, 1H), 7.52-7.58 (m, 2H), 7.41 (d, J=6.85 Hz, 1H), 7.09-7.16 (m, 1H), 5.91-6.17 (m, 1H), 4.16 (t, J=5.99 Hz, 2H), 3.90-4.03 (m, 2H), 3.53-3.60 (m, 2H), 3.03 (t, J=6.60 Hz, 2H), 2.54-2.67 (m, 6H), 2.01-2.15 (m, 4H); LCMS: [M+H]+=517.47
Prepared from 1-(5-bromo-2-(4,4-difluorobutoxy)benzyl)-4-methoxypiperidine (55 mg, 0.133 mmol, in turn made using 5-bromo-2-(4,4-difluorobutoxy)benzaldehyde and 4-methoxypiperidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (66.4 mg, 0.173 mmol) to give the title compound as a white solid (62 mg, 78% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.03 (d, J=7.95 Hz, 1H), 7.62-7.67 (m, 1H), 7.41-7.52 (m, 4H), 7.00 (d, J=8.56 Hz, 1H), 5.89-6.15 (m, 1H), 4.08 (t, J=5.93 Hz, 2H), 3.59-3.65 (m, 2H), 3.54 (t, J=6.60 Hz, 2H), 3.31-3.33 (m, 3H), 3.21-3.28 (m, 1H), 3.05 (t, J=6.60 Hz, 2 H), 2.74-2.91 (m, 2H), 2.29 (t, J=8.93 Hz, 2H), 1.95-2.09 (m, 4H), 1.85-1.93 (m, 2H), 1.57 (d, J=9.29 Hz, 2H); LCMS: [M+H]+=569.61.
Prepared from 1-(5-bromo-2-(4,4-difluorobutoxy)benzyl)-4-methoxypiperidine (55 mg, 0.133 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (69.5 mg, 0.173 mmol) to afford the title compound as an off white solid (31 mg, 38% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 7.75 (d, J=10.03 Hz, 1H), 7.65 (d, J=8.80 Hz, 1H), 7.48 (s, 1H), 7.39-7.45 (m, 2H), 7.39-7.45 (m, 2 H), 7.01-7.05 (m, 1H), 5.91-6.17 (m, 1H), 4.10 (t, J=5.69 Hz, 2H), 3.62 (s, 2H), 3.56 (t, J=6.66 Hz, 2H), 3.32-3.33 (m, 3H), 3.23-3.30 (m, 1H), 3.03 (t, J=6.48 Hz, 2H), 2.83 (br. s., 2H), 2.26-2.37 (m, 2H), 1.98-2.13 (m, 4H), 1.91 (d, J=11.37 Hz, 2H), 1.59 (d, J=9.29 Hz, 2H); LCMS: [M+H]+=587.54
To a solution of 2-(6-amino-2-fluoro-5-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-5-(3,6-dihydro-2H-pyran-4-yl)benzaldehyde (70 mg, 0.152 mmol) in THF (3 ml) at r.t., NaBH4 (57.4 mg, 1.517 mmol) was added. The reaction mixture was stirred for 5 min, quenched with MeOH (3 mL) and stirred at r.t. overnight. The reaction mixture was concentrated and the residue was diluted with water, the resulting precipitate was filtered, rinsed with water and ether to give the title compound (60 mg, 84% yield). 1H NMR (500 MHz, METHANOL-d4) δ=7.75 (d, J=10.0 Hz, 1H), 7.69 (d, J=1.7 Hz, 1H), 7.50 (d, J=9.4 Hz, 1H), 7.46-7.40 (m, 2H), 7.25 (d, J=7.9 Hz, 1H), 6.29 (td, J=1.4, 2.9 Hz, 1H), 4.56 (s, 2H), 4.34 (q, J=2.7 Hz, 2H), 3.97 (t, J=5.5 Hz, 2H), 3.55 (t, J=6.7 Hz, 2H), 3.03 (t, J=6.6 Hz, 2H), 2.62-2.56 (m, 2H); [M+H]+=464.45.
Step 1: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,8-difluoro-3-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (220 mg, 0.694 mmol) and (4,8-difluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (199 mg, 0.833 mmol) to give the product (187 mg, 70% yield) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.27 (br. s., 1H), 7.82 (d, J=8.93 Hz, 1H), 7.46 (d, J=0.98 Hz, 1H), 7.31 (d, J=12.47 Hz, 1H), 6.58 (s, 2H), 2.22 (d, J=3.06 Hz, 3H); LCMS: [M+H]+=384.22.
Step 2: 6-(2-amino-5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-4,8-difluoro-3-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,8-difluoro-3-methylisoquinolin-1(2H)-one (35 mg, 0.091 mmol) and 1-(cyclopropylmethyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (37.4 mg, 0.109 mmol) to give the title compound (16.3 mg, 34.4% yield) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) b ppm 11.25 (br. s., 1H), 7.69 (d, J=10.15 Hz, 1H), 7.53 (d, J=0.98 Hz, 1H), 7.37-7.45 (m, 3 H), 6.98 (d, J=8.93 Hz, 2H), 6.36 (s, 2H), 3.14-3.20 (m, 4H), 2.56-2.59 (m, 4H), 2.20-2.25 (m, 5H), 0.82-0.89 (m, 1H), 0.46-0.50 (m, 2H), 0.08-0.12 (m, 2H); LCMS: [M+H]+=520.54.
Step 1: 1-(5-bromo-2-(cyclopropylmethoxy)benzyl)-4-methoxypiperidine
Prepared from 5-bromo-2-((tetrahydro-2H-pyran-4-yl)oxy)benzaldehyde (150 mg, 0.526 mmol) and dimethylamine, 2.0M solution in THF (1.052 ml, 2.104 mmol) to give the product (169 mg, 97% yield). LCMS: [M+H]+=314.26.
Step 2: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-((tetrahydro-2H-pyran-4-yl)oxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(5-bromo-2-((tetrahydro-2H-pyran-4-yl)oxy)phenyl)-N,N-dimethylmethanamine (55 mg, 0.175 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (87 mg, 0.228 mmol) to afford the title compound as a beige solid (63 mg, 70% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.94 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.56-7.61 (m, 1H), 7.43-7.48 (m, 3 H), 7.38 (d, J=8.68 Hz, 1H), 7.05-7.12 (m, 1H), 6.22 (s, 2H), 4.64 (tt, J=7.64, 3.73 Hz, 1 H), 3.81-3.90 (m, 2H), 3.52 (ddd, J=11.40, 8.28, 3.06 Hz, 2H), 3.43 (s, 2H), 3.39-3.42 (m, 2H), 3.40 (dd, J=6.54, 2.51 Hz, 2H), 2.96 (t, J=6.54 Hz, 2H), 2.15-2.23 (m, 6H), 1.93-2.00 (m, 2H), 1.59-1.69 (m, 2H); LCMS: [M+H]+=491.59.
Prepared from (5-bromo-2-((tetrahydro-2H-pyran-4-yl)oxy)phenyl)-N,N-dimethylmethanamine (55 mg, 0.175 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (91 mg, 0.228 mmol) to afford the title compound as an off white solid (31 mg, 33% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.11 (br. s., 1H), 7.60 (dd, J=18.77, 10.09 Hz, 2H), 7.41-7.47 (m, 2H), 7.36 (d, J=8.44 Hz, 1H), 7.08 (d, J=8.68 Hz, 1H), 6.23 (s, 2H), 4.63 (dt, J=7.55, 3.87 Hz, 1H), 3.81-3.87 (m, 2H), 3.51-3.56 (m, 2H), 3.43 (s, 2H), 3.40-3.41 (m, 2H), 2.92 (t, J=6.36 Hz, 2H), 2.18 (s, 6H), 1.93-2.00 (m, 2H), 1.59-1.68 (m, 2H); LCMS: [M+H]+=509.51.
Prepared from 1-(5-bromo-2-((tetrahydro-2H-pyran-4-yl)methoxy)phenyl)-N,N-dimethylmethanamine (80 mg, 0.171 mmol, in turn made from 5-bromo-2-((tetrahydro-2H-pyran-4-yl)methoxy)benzaldehyde and dimethylamine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (85 mg, 0.222 mmol) to afford the title compound as an off white solid (67.5 mg, 75% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.57 (d, J=10.03 Hz, 1H), 7.42-7.49 (m, 3H), 7.39 (d, J=8.44 Hz, 1H), 7.01 (d, J=8.44 Hz, 1H), 6.22 (br. s., 2H), 3.88-3.93 (m, 2H), 3.86 (d, J=5.99 Hz, 2H), 3.42 (br. s., 4H), 3.38 (br. s., 2H), 2.96 (t, J=6.36 Hz, 2H), 2.17 (s, 6H), 2.03 (d, J=4.65 Hz, 1H), 1.70 (d, J=12.59 Hz, 2 H), 1.39 (qd, J=12.17, 4.34 Hz, 2H); LCMS: [M+H]+=505.50.
Prepared from 1-(5-bromo-2-((tetrahydro-2H-pyran-4-yl)methoxy)phenyl)-N,N-dimethylmethanamine (80 mg, 0.171 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (89 mg, 0.222 mmol) to afford the title compound as a white solid (29 mg, 31% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.11 (br. s., 1H), 7.56-7.65 (m, 2H), 7.44 (br. s., 2H), 7.37 (d, J=8.44 Hz, 1H), 7.01 (d, J=8.44 Hz, 1H), 6.23 (br. s., 2H), 3.90 (d, J=9.54 Hz, 2H), 3.86 (d, J=5.75 Hz, 2H), 3.41 (br. s., 4H), 2.93 (br. s., 2H), 2.17 (s, 6H), 2.02 (br. s., 1H), 1.70 (d, J=12.72 Hz, 2H), 1.33-1.44 (m, 2H); LCMS: [M+H]+=523.61
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,8-difluoro-3-methylisoquinolin-1(2H)-one (35 mg, 0.091 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (37.9 mg, 0.109 mmol) to give the title compound (5.7 mg, 12% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) b ppm 11.25 (br. s., 1H), 7.69 (d, J=10.15 Hz, 1H), 7.53 (s, 2H), 7.37-7.45 (m, 2H), 7.13 (d, J=8.31 Hz, 1H), 6.43 (s, 2H), 3.71-3.77 (m, 4H), 3.44 (s, 2H), 2.91-2.97 (m, 4H), 2.22 (d, J=2.81 Hz, 3H), 2.18 (s, 6H); LCMS: [M+H]+=524.46.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,8-difluoro-3-methylisoquinolin-1(2H)-one (35 mg, 0.091 mmol) and N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (37.8 mg, 0.109 mmol) to give the title compound (15.8 mg, 33.2% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.26 (br. s., 1H), 7.73 (d, J=10.03 Hz, 1H), 7.59-7.64 (m, 1 H), 7.54-7.58 (m, 1H), 7.53 (s, 1H), 7.34-7.41 (m, 2H), 6.47 (br. s., 2H), 3.95 (dd, J=10.70, 3.24 Hz, 2H), 3.44 (t, J=10.58 Hz, 4H), 3.14-3.24 (m, 1H), 2.22 (d, J=2.81 Hz, 3H), 2.17 (br. s., 6H), 1.66-1.76 (m, 2H), 1.57-1.65 (m, 2H); LCMS: [M+H]+=523.41.
Prepared from 5-bromo-2-methoxyphenyl)methanol (60 mg, 0.235 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (117 mg, 0.305 mmol) to afford the title compound as an off white solid (74.5 mg, 77% yield). 1H NMR ((500 MHz, METHANOL-d4) δ=8.10-8.01 (m, 1H), 7.69-7.62 (m, 1H), 7.59-7.55 (m, 1H), 7.54-7.50 (m, 1H), 7.49-7.46 (m, 1H), 7.46-7.42 (m, 1H), 7.06-6.96 (m, 1H), 4.72-4.67 (m, 2H), 3.92-3.86 (m, 3H), 3.60-3.53 (m, 2H), 3.11-3.05 (m, 2H); LCMS: [M+H]+=394.37.
Step 1: 6-bromo-4-methoxyisoquinolin-1(2H)-one
A vial was charged with 6-bromo-2H-isoquinolin-1-one (300 mg, 1.339 mmol), methanol (2 ml) and methanesulfonic acid (0.087 ml, 1.339 mmol) then cooled to 0° C. A solution of iodobenzene diacetate (474 mg, 1.473 mmol) in methanol (2 ml) was added slowly. The reaction was warmed to room temperature and stirred for 1 hour then heated at 50° C. overnight. The reaction was concentrated in vacuo and triturated with isopropanol. The filtered solid was vacuum dried to afford 6-bromo-4-methoxyisoquinolin-1(2H)-one (257 mg, 1.011 mmol, 76% yield) as a pink solid. LCMS [M+H]+=254.20.
Step 2: 4-methoxy-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one
Prepared from 6-bromo-4-methoxyisoquinolin-1(2H)-one (70 mg, 0.276 mmol) to give the boronate which was used in the next step without further purification. LCMS: [M+H]+=302.23.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4-methoxyisoquinolin-1(2H)-one
Prepared from 4-methoxy-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoquinolin-1(2H)-one (36.8 mg, 0.122 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (40 mg, 0.102 mmol) to give the title compound (23.8 mg, 48.0% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 10.95 (d, J=4.89 Hz, 1H), 8.25 (d, J=8.31 Hz, 1H), 7.90 (d, J=1.22 Hz, 1H), 7.67 (dd, J=8.25, 1.53 Hz, 1H), 7.62 (d, J=10.15 Hz, 1H), 7.40 (d, J=7.95 Hz, 2H), 6.96 (d, J=8.80 Hz, 2H), 6.77 (d, J=5.38 Hz, 1H), 6.20 (s, 2H), 3.79 (s, 3H), 3.12-3.17 (m, 4H), 2.67 (dt, J=13.11, 6.59 Hz, 1H), 2.56-2.59 (m, 4H), 1.00 (d, J=6.48 Hz, 6H); LCMS: [M+H]+=488.50.
Step 1: 6-bromo-4-chloro-3-methylisoquinolin-1(2H)-one
To a solution of 6-bromo-3-methyl-2H-isoquinolin-1-one (1.0 g, 4.20 mmol) in N,N-dimethylacetamide (14 ml) was added N-chlorosuccinimide (0.673 g, 5.04 mmol). The mixture was stirred at 50° C. for 8 hours. The reaction was cooled to RT and diluted with water. The suspension was filtered and washed with water. The solid was further triturated from MeOH and dried under vacuum to give the product (1.12 g, 98% yield) as a white solid. LCMS: [M+H]+=272.20.
Step 2: (4-chloro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-4-chloro-3-methylisoquinolin-1(2H)-one (1.1 g, 4.04 mmol) to give a mixture of boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+=238.34 Boronic acid, 320.34 Boronate.
Step 3: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-chloro-3-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (425 mg, 1.341 mmol) and (4-chloro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (446 mg, 1.878 mmol) to give the product (112 mg, 21.8% yield) as a beige solid. LCMS [M+H]+=382.21.
Step 4: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4-chloro-3-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-chloro-3-methylisoquinolin-1(2H)-one (30 mg, 0.078 mmol) and 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (31.1 mg, 0.094 mmol) to give the title compound (18.3 mg, 46% yield) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.67 (br. s., 1H), 8.25 (d, J=8.19 Hz, 1H), 7.87 (s, 1H), 7.62-7.67 (m, 2H), 7.40 (d, J=8.07 Hz, 2H), 6.97 (d, J=8.80 Hz, 2H), 6.24 (s, 2H), 3.12-3.17 (m, 4H), 2.64-2.70 (m, 1H), 2.55-2.59 (m, 4H), 2.38 (s, 3H), 1.00 (d, J=6.60 Hz, 6H); LCMS: [M+H]+=506.56.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (30.0 mg, 0.085 mmol) and 8-methyl-2-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-5-oxa-2,8-diazaspiro[3.5]nonane (40 mg, 0.085 mmol) to give the title compound (12.3 mg, 29.9% yield). 1H NMR (500 MHz, METHANOL-d4) δ=7.75 (d, J=10.0 Hz, 1H), 7.59 (d, J=9.8 Hz, 1H), 7.41 (d, J=6.8 Hz, 1H), 7.38 (d, J=8.2 Hz, 2H), 6.58 (d, J=8.4 Hz, 2H), 3.91 (d, J=7.9 Hz, 2H), 3.76 (t, J=4.6 Hz, 2H), 3.70 (d, J=7.8 Hz, 2H), 3.56 (t, J=6.7 Hz, 2H), 3.03 (t, J=6.6 Hz, 2H), 2.70 (br s, 2H), 2.48 (br s, 2H), 2.38 (s, 3H); LCMS: [M+H]+=492.46;
Prepared from 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)-4-methoxypiperidine (55 mg, 0.142 mmol, in turn made from 5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzaldehyde and 4-methoxypiperidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (70.7 mg, 0.184 mmol) to give the title compound as a beige solid (27 mg, 33% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.63 (d, J=10.03 Hz, 1H), 7.39-7.50 (m, 3H), 7.31-7.38 (m, 2H), 6.29 (s, 2H), 3.92-4.02 (m, 2H), 3.50 (s, 2H), 3.39-3.46 (m, 4H), 3.14-3.24 (m, 5H), 2.95 (t, J=6.42 Hz, 2H), 2.64 (br. s., 2H), 2.10 (t, J=9.48 Hz, 2H), 1.75-1.85 (m, 2H), 1.67-1.75 (m, 2H), 1.59-1.66 (m, 2H), 1.33 (d, J=9.41 Hz, 2H); LCMS: [M+H]+=545.53.
Prepared from 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)-4-methoxypiperidine (55 mg, 0.142 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (74 mg, 0.184 mmol) to give the title compound as a white solid (23 mg, 27% yield). 1H NMR (500 MHz, DMSO-d6 b ppm 8.12 (br. s., 1H), 7.58-7.67 (m, 2H), 7.42 (t, J=7.58 Hz, 2H), 7.34 (d, J=7.34 Hz, 2H), 6.29 (s, 2H), 3.92-4.03 (m, 2H), 3.49 (s, 2H), 3.39-3.46 (m, 4H), 3.39-3.46 (m, 4H), 3.13-3.22 (m, 5H), 2.90-2.97 (m, 2H), 2.64 (br. s., 2H), 2.10 (t, J=8.93 Hz, 2H), 1.79 (d, J=9.90 Hz, 2H), 1.68-1.76 (m, 2H), 1.61-1.67 (m, 2H), 1.32 (d, J=8.80 Hz, 2H); LCMS: [M+H]+=563.60.
Prepared from 4-(4-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.169 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and 3-methoxypyrrolidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (84 mg, 0.220 mmol) to give the title compound as a white solid (48 mg, 51% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 5 7.9-8.0 (m, 1H), 7.91 (d, 1H, J=7.9 Hz), 7.6-7.6 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 1H), 7.1-7.2 (m, 1H), 6.2-6.3 (m, 2H), 3.8-3.9 (m, 1H), 3.7-3.8 (m, 4H), 3.6-3.7 (m, 2H), 3.4-3.4 (m, 2H), 3.1-3.2 (m, 3H), 2.9-3.0 (m, 6H), 2.7-2.7 (m, 1H), 2.6-2.7 (m, 1H), 2.55 (br d, 1H, J=1.7 Hz), 2.4-2.5 (m, 1H), 1.9-2.0 (m, 1H), 1.6-1.7 (m, 1H); LCMS: [M+H]+=532.58.
Prepared from 4-(4-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.169 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (84 mg, 0.220 mmol) to give the title compound as a white solid (48 mg, 51% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 5 7.9-8.0 (m, 1H), 7.91 (d, 1H, J=7.9 Hz), 7.6-7.6 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 1H), 7.1-7.2 (m, 1H), 6.2-6.3 (m, 2H), 3.8-3.9 (m, 1H), 3.7-3.8 (m, 4H), 3.6-3.7 (m, 2H), 3.4-3.4 (m, 2H), 3.1-3.2 (m, 3H), 2.9-3.0 (m, 6H), 2.7-2.7 (m, 1H), 2.6-2.7 (m, 1H), 2.55 (br d, 1H, J=1.7 Hz), 2.4-2.5 (m, 1H), 1.9-2.0 (m, 1H), 1.6-1.7 (m, 1H); LCMS: [M+H]+=532.58.
Step 1: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (50 mg, 0.142 mmol), and 4-piperazinylphenylboronic acid, pinacol ester (53.2 mg, 0.185 mmol) to give the title compound as a beige solid (30 mg, 49%); LCMS: [M+H]+=434.43.
Step 2: 6-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (30 mg, 0.069 mmol) and 4-bromo-1,1-difluorobutane (23.95 mg, 0.138 mmol) to give the title compound as a pale-yellow solid (10 mg, 26% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.34-11.10 (m, 1H), 8.30-8.21 (m, 1H), 7.92-7.82 (m, 1H), 7.76-7.66 (m, 2H), 7.43 (br d, J=7.3 Hz, 3H), 7.02-6.91 (m, 2H), 6.32-6.24 (m, 2H), 6.24-5.97 (m, 1H), 3.17 (br s, 4H), 2.58-2.52 (m, 4H), 2.39-2.33 (m, 2H), 1.92-1.79 (m, 2H), 1.63-1.53 (m, 2H); LCMS: [M+H]+=526.50.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (35 mg, 0.101 mmol) and 1-cyclobutyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (41.3 mg, 0.121 mmol) to give the product (22.8 mg, 46.9% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.09 (d, J=5.01 Hz, 1 H), 8.27 (d, J=8.19 Hz, 1H), 7.72 (s, 1H), 7.60-7.69 (m, 2H), 7.41 (d, J=8.07 Hz, 2H), 7.04 (d, J=4.89 Hz, 1H), 6.97 (d, J=8.68 Hz, 2H), 6.24 (s, 2H), 3.15 (d, J=4.28 Hz, 4H), 2.73 (quin, J=7.64 Hz, 1H), 2.37 (d, J=4.52 Hz, 4H), 2.24 (s, 3H), 1.93-2.03 (m, 2H), 1.81 (quin, J=9.45 Hz, 2H), 1.59-1.71 (m, 2H); LCMS: [M+H]+=484.51.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (35 mg, 0.099 mmol) and 1-cyclobutyl-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (40.8 mg, 0.119 mmol) to give the title compound (16.7 mg, 34.5% yield) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.16 (br. s., 1H), 8.26 (dd, J=8.31, 1.71 Hz, 1H), 7.82 (d, J=0.98 Hz, 1H), 7.73 (dd, J=8.31, 1.35 Hz, 1H), 7.67 (d, J=10.15 Hz, 1H), 7.42 (d, J=7.83 Hz, 3H), 6.97 (d, J=8.80 Hz, 2H), 6.29 (s, 2H), 3.11-3.18 (m, 4H), 2.73 (quin, J=7.76 Hz, 1H), 2.35-2.41 (m, 4H), 1.95-2.02 (m, 2H), 1.77-1.86 (m, 2H), 1.60-1.70 (m, 2H); LCMS: [M+H]+=488.45.
Step 1: 6-bromo-4,7-difluoroisoquinolin-1(2H)-one
A RBF was charged with 6-bromo-7-fluoro-2H-isoquinolin-1-one (800 mg, 3.31 mmol) and Selectfluor™ fluorinating reagent (1405 mg, 3.97 mmol). Methanol (8 ml) and acetonitrile (8 ml) were added, and the reaction was heated to 50° C. and stirred for 1 h. The reaction was concentrated in vacuo, dissolved in 1,2-dichloroethane (16 mL), and phosphorous (V) oxychloride (0.618 ml, 6.61 mmol) was added. The reaction was stirred at 50° C. for 1 h then quenched into ice water and extracted with CH2Cl2 (2x). The combined organic layers were dried with anhydrous Na2SO4 and concentrated to afford the product (710 mg, 83%) as a white solid. The material was used in the next step without further purification. LCMS: [M−H]−=258.09.
Step 2: (4,7-difluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-4,7-difluoroisoquinolin-1(2H)-one (710 mg, 2.73 mmol) to give the boronic acid which was used in the next step without further purification. LCMS: [M+H]+ 226.12.
Step 3: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,7-difluoroisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (352 mg, 1.111 mmol) and (4,7-difluoro-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (300 mg, 1.333 mmol) to give the product (246 mg, 59.8% yield) as a beige solid. LCMS: [M+H]+=370.18.
Step 4: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4,7-difluoroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,7-difluoroisoquinolin-1(2H)-one (35 mg, 0.095 mmol) and 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (37.5 mg, 0.113 mmol) to give the title compound (17.2 mg, 36.9% yield) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.29 (br. s., 1H), 7.96 (d, J=9.29 Hz, 1H), 7.81 (d, J=6.72 Hz, 1H), 7.68 (d, J=10.15 Hz, 1 H), 7.36-7.44 (m, 3H), 6.96 (d, J=8.68 Hz, 2H), 6.28 (s, 2H), 3.12-3.16 (m, 4H), 2.64-2.70 (m, 1H), 2.56-2.59 (m, 4H), 1.00 (d, J=6.48 Hz, 6H); LCMS [M+H]+ 494.53.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,7-difluoroisoquinolin-1(2H)-one (35 mg, 0.095 mmol) and 1-(cyclopropylmethyl)-4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperazine (38.8 mg, 0.113 mmol) to give the title compound (8.2 mg, 17% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.27 (br. s., 1H), 7.94-7.98 (m, 1H), 7.81 (d, J=6.85 Hz, 1H), 7.68 (d, J=10.15 Hz, 1H), 7.37-7.43 (m, 3H), 6.97 (d, J=8.93 Hz, 2H), 6.28 (s, 2H), 3.15-3.20 (m, 4H), 2.56-2.59 (m, 4 H), 2.22 (d, J=6.60 Hz, 2H), 0.81-0.91 (m, 1H), 0.45-0.51 (m, 2H), 0.07-0.12 (m, 2H); LCMS: [M+H]+=506.50.
Prepared from 4-(2-(azetidin-1-ylmethyl)-4-bromophenyl)morpholine (50 mg, 0.153 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and azetidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (76 mg, 0.198 mmol) to yield the title compound as a white solid (67 mg, 86% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.00-7.93 (m, 1H), 7.93-7.87 (m, 1H), 7.61-7.55 (m, 1H), 7.50-7.43 (m, 3H), 7.41-7.34 (m, 1H), 7.13-7.07 (m, 1H), 6.26 (s, 2H), 3.79-3.72 (m, 4H), 3.63-3.57 (m, 2H), 3.44-3.39 (m, 2H), 3.20-3.13 (m, 4H), 2.98-2.94 (m, 2H), 2.94-2.89 (m, 4H), 2.04-1.91 (m, 2H); LCMS: [M+H]+=488.58.
Prepared from 4-(2-(azetidin-1-ylmethyl)-4-bromophenyl)morpholine (50 mg, 0.153 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.198 mmol) to yield the title compound as a white solid (42 mg, 52% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.24-8.05 (m, 1H), 7.69-7.55 (m, 2H), 7.50-7.45 (m, 1H), 7.45-7.39 (m, 1H), 7.39-7.32 (m, 1H), 7.16-7.05 (m, 1H), 6.34-6.22 (m, 2H), 3.74 (br s, 4H), 3.59 (br s, 2H), 3.46-3.40 (m, 2H), 3.16 (br s, 4H), 2.92 (br s, 6H), 1.98 (br s, 2H); LCMS: [M+H]+=506.50
Step 1: 6-bromo-3-morpholinopicolinaldehyde
Prepared aby the reaction between 2-bromo-5-fluoro-6-formylpyridine (2.5 g, 12.25 mmol) and morpholine (1.269 ml, 14.71 mmol) at 85° C. for 6 h. The title compound was isolated as a pale-yellow oil (2.747 g, 79% yield). LCMS: [M+H]+=271.18
Step 2: 1-(6-bromo-3-morpholinopyridin-2-yl)-N,N-dimethylmethanamine
Prepared using 6-bromo-3-morpholinopicolinaldehyde (500 mg, 1.752 mmol) and Dimethylamine, 2.0M solution in THF (0.876 ml, 1.752 mmol). Silica gel chromatography (eluting with CH2Cl2 containing 0-2% MeOH and 0-0.2% NH4OH) afforded the title product as a cream colored solid (336 mg, 62% yield). LCMS: [M+H]+=300.35
Step 3: 6-(6′-amino-6-((dimethylamino)methyl)-2′-fluoro-5-morpholino-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(6-bromo-3-morpholinopyridin-2-yl)-N,N-dimethylmethanamine (50 mg, 0.162 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (81 mg, 0.211 mmol) to yield the title compound as a white solid (61 mg, 75% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.12-8.03 (m, 1H), 7.99-7.94 (m, 1H), 7.94-7.89 (m, 1H), 7.68-7.60 (m, 1H), 7.58-7.51 (m, 1H), 7.45-7.37 (m, 2H), 6.55-6.34 (m, 2H), 3.81-3.72 (m, 4H), 3.56-3.51 (m, 2H), 3.44-3.40 (m, 2H), 3.11-3.01 (m, 4H), 2.96 (br t, J=6.4 Hz, 2H), 2.30-2.16 (m, 6H); LCMS: [M+H]+=477.49.
Prepared from N-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)-N-methylethanamine (55 mg, 0.167 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (87 mg, 0.218 mmol) to yield the title compound as a white solid (47 mg, 53% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.17-8.07 (m, 1H), 7.65-7.58 (m, 2H), 7.45-7.40 (m, 2H), 7.39-7.36 (m, 1H), 7.35-7.32 (m, 1H), 6.43-6.23 (m, 2H), 4.00-3.94 (m, 2H), 3.51-3.47 (m, 2H), 3.46-3.40 (m, 4H), 3.27-3.19 (m, 1H), 2.94-2.89 (m, 2H), 2.40 (q, J=7.1 Hz, 2H), 2.13-2.07 (m, 3H), 1.76-1.67 (m, 2H), 1.65-1.60 (m, 2H), 1.08-1.02 (m, 3H); LCMS: [M+H]+=507.51.
Prepared from N-(5-bromo-2-morpholinobenzyl)-2-methoxy-N-methylethan-1-amine (60 mg, 0.175 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and N-(2-methoxyethyl)methylamine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (87 mg, 0.227 mmol) to yield the title compound as a white solid (68 mg, 71% yield). 1H NMR (500 MHz, DMSO-d6) δ=7.98-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.61-7.56 (m, 2H), 7.49-7.39 (m, 3H), 7.18-7.10 (m, 1H), 6.30-6.19 (m, 2H), 3.77-3.71 (m, 4H), 3.60-3.53 (m, 2H), 3.45-3.42 (m, 2H), 3.28 (s, 2H), 3.19-3.14 (m, 3H), 2.98-2.94 (m, 2H), 2.94-2.89 (m, 4H), 2.56-2.52 (m, 2H), 2.25-2.19 (m, 3H); LCMS: [M+H]+=520.61.
Prepared from N-(5-bromo-2-morpholinobenzyl)-2-methoxy-N-methylethan-1-amine (60 mg, 0.175 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (87 mg, 0.227 mmol) to yield the title compound as a white solid (68 mg, 71% yield). 1H NMR (500 MHz, DMSO-d6) b=7.98-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.61-7.56 (m, 2H), 7.49-7.39 (m, 3H), 7.18-7.10 (m, 1H), 6.30-6.19 (m, 2H), 3.77-3.71 (m, 4H), 3.60-3.53 (m, 2H), 3.45-3.42 (m, 2H), 3.28 (s, 2H), 3.19-3.14 (m, 3H), 2.98-2.94 (m, 2H), 2.94-2.89 (m, 4H), 2.56-2.52 (m, 2H), 2.25-2.19 (m, 3H); LCMS: [M+H]+=520.61.
Prepared from 1-(5-bromo-2-(2,2-difluoroethoxy)phenyl)-N,N-dimethylmethanamine (55 mg, 0.178 mmol, in turn made from 5-bromo-2-(2,2-difluoroethoxy)benzaldehyde and dimethylamine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (88 mg, 0.231 mmol) to afford the title compound as an off white solid (69 mg, 78% yield). 1H NMR (500 MHz, DMSO-d6) δ=7.99-7.94 (m, 1H), 7.93-7.90 (m, 1H), 7.63-7.58 (m, 1H), 7.50-7.46 (m, 2H), 7.46-7.41 (m, 2H), 7.13-7.08 (m, 1H), 6.54-6.30 (m, 1H), 6.29-6.22 (m, 2H), 4.42-4.29 (m, 2H), 3.47-3.43 (m, 2H), 3.42-3.40 (m, 2H), 2.98-2.94 (m, 2H), 2.21-2.15 (m, 6H); LCMS: [M+H]+=471.53.
Prepared from 1-(5-bromo-2-(2,2-difluoroethoxy)phenyl)-N,N-dimethylmethanamine (55 mg, 0.178 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (93 mg, 0.231 mmol) to afford the title compound as a white solid (45 mg, 49% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.17-8.05 (m, 1H), 7.66-7.55 (m, 2H), 7.50-7.45 (m, 1H), 7.45-7.42 (m, 1H), 7.41 (br d, J=8.7 Hz, 1H), 7.16-7.03 (m, 1H), 6.52-6.29 (m, 1H), 6.26 (s, 1H), 4.39-4.28 (m, 2H), 3.46-3.43 (m, 2H), 3.42-3.39 (m, 2H), 2.96-2.89 (m, 2H), 2.23-2.11 (m, 6H); LCMS: [M+H]+=489.52.
Prepared from 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)pyrrolidine (60 mg, 0.148 mmol, in turn made from 5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzaldehyde and pyrrolidine (0.275 ml, 3.34 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (73.7 mg, 0.192 mmol) to yield the title compound as an off white solid (67 mg, 86% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.00-7.93 (m, 1H), 7.91 (br d, J=7.8 Hz, 1H), 7.67-7.60 (m, 1H), 7.50-7.40 (m, 4H), 7.33 (br d, J=7.8 Hz, 1H), 6.36-6.20 (m, 2H), 3.96 (br d, J=8.6 Hz, 2H), 3.69-3.64 (m, 2H), 3.48-3.40 (m, 4H), 3.21-3.15 (m, 1H), 2.99-2.92 (m, 2H), 2.45 (br s, 4H), 1.75-1.62 (m, 8H); LCMS: [M+H]+=501.62.
Prepared from 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)pyrrolidine (60 mg, 0.148 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (77 mg, 0.192 mmol) to yield the title compound as an off white solid (42 mg, 52% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.11 (br s, 1H), 7.66-7.58 (m, 2H), 7.45-7.38 (m, 3H), 7.35-7.30 (m, 1H), 6.35-6.23 (m, 2H), 3.99-3.92 (m, 2H), 3.65 (s, 2H), 3.48-3.40 (m, 4H), 3.23-3.16 (m, 1H), 2.95-2.90 (m, 2H), 2.45 (br s, 4H), 1.72-1.60 (m, 8H); LCMS: [M+H]+=519.60.
Prepared from 4-(4-bromo-2-(piperidin-1-ylmethyl)phenyl)morpholine (60 mg, 0.150 mmol, in turn made using 5-bromo-2-morpholinobenzaldehyde and piperidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (74.9 mg, 0.195 mmol) to yield the title compound as a white solid (54 mg, 66% yield). 1H NMR (500 MHz, DMSO-d6) δ=7.98-7.93 (m, 1H), 7.93-7.89 (m, 1H), 7.63-7.57 (m, 1H), 7.55-7.50 (m, 1H), 7.49-7.46 (m, 1H), 7.45-7.43 (m, 1H), 7.43-7.38 (m, 1H), 7.18-7.11 (m, 1H), 6.30-6.21 (m, 2H), 3.78-3.71 (m, 4H), 3.51-3.46 (m, 2H), 3.45-3.39 (m, 2H), 3.01-2.92 (m, 6H), 2.45-2.33 (m, 4H), 1.51-1.43 (m, 4H), 1.42-1.33 (m, 2H); LCMS: [M+H]+=516.59.
Prepared from 4-(4-bromo-2-(piperidin-1-ylmethyl)phenyl)morpholine (60 mg, 0.150 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (78 mg, 0.195 mmol) to yield the title compound as a white solid (31 mg, 37% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.17-8.04 (m, 1H), 7.66-7.55 (m, 2H), 7.53-7.48 (m, 1H), 7.46-7.41 (m, 1H), 7.41-7.36 (m, 1H), 7.18-7.10 (m, 1H), 6.45-6.09 (m, 2H), 3.82-3.71 (m, 4H), 3.48 (s, 2H), 3.44-3.39 (m, 2H), 2.99-2.87 (m, 6H), 2.43-2.27 (m, 4H), 1.55-1.43 (m, 4H), 1.42-1.35 (m, 2H); LCMS: [M+H]+=534.58.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,7-difluoroisoquinolin-1(2H)-one (35 mg, 0.095 mmol) and N,N-dimethyl-1-(2-morpholino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (39.3 mg, 0.113 mmol) to give the product (9.1 mg, 19% yield) as a grey solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.29 (br. s., 1H), 7.97 (d, J=9.90 Hz, 1H), 7.82 (d, J=6.72 Hz, 1H), 7.69 (d, J=10.03 Hz, 1 H), 7.52 (br. s., 1H), 7.42 (d, J=5.26 Hz, 2H), 7.13 (d, J=8.19 Hz, 1H), 6.37 (br. s., 2H), 3.74 (br. s., 4H), 3.45 (br. s., 2H), 2.93 (br. s., 4H), 2.19 (br. s., 6H); LCMS: [M+H]+=510.58.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4,7-difluoroisoquinolin-1(2H)-one (35 mg, 0.095 mmol) and N,N-dimethyl-1-(2-(tetrahydro-2H-pyran-4-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (39.2 mg, 0.113 mmol) to give the product (7.4 mg, 15% yield) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.29 (d, J=5.50 Hz, 1H), 7.96 (d, J=10.03 Hz, 1H), 7.82 (d, J=6.72 Hz, 1 H), 7.73 (d, J=10.03 Hz, 1H), 7.37-7.46 (m, 3H), 7.34 (d, J=8.19 Hz, 1H), 6.39 (br. s., 2 H), 3.95 (dd, J=10.88, 3.18 Hz, 2H), 3.43 (d, J=10.39 Hz, 4H), 3.19 (t, J=11.68 Hz, 1H), 2.16 (br. s., 6H), 1.66-1.75 (m, 2H), 1.58-1.65 (m, 2H); LCMS: [M+H]+=509.57.
Prepared from (R)-4-(4-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.160 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and (R)-3-methoxy-pyrrolidine hydrochloride) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.209 mmol) to yield the title compound as a white solid (55 mg, 61% yield). 1H NMR (500 MHz, DMSO-d6) δ=7.99-7.93 (m, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.63-7.57 (m, 1H), 7.55-7.49 (m, 1H), 7.47 (br d, J=7.9 Hz, 1H), 7.45 (s, 1H), 7.41 (br d, J=7.6 Hz, 1H), 7.18-7.07 (m, 1H), 6.44-6.18 (m, 2H), 3.92-3.85 (m, 1H), 3.79-3.73 (m, 4H), 3.64 (br s, 2H), 3.43-3.41 (m, 2H), 3.16-3.12 (m, 3H), 2.98-2.92 (m, 6H), 2.72-2.62 (m, 4H), 2.03-1.93 (m, 1H), 1.70-1.58 (m, 1H); LCMS: [M+H]+=532.64.
Prepared from (R)-4-(4-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.160 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (84 mg, 0.209 mmol) to yield the title compound as a white solid (40 mg, 43% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.17-8.06 (m, 1H), 7.66-7.56 (m, 2H), 7.51 (br s, 1H), 7.46-7.42 (m, 1H), 7.41-7.33 (m, 1H), 7.19-7.05 (m, 1H), 6.36-6.14 (m, 2H), 3.91-3.84 (m, 1H), 3.75 (br s, 4H), 3.64 (br s, 2H), 3.44-3.40 (m, 2H), 3.14 (br s, 3H), 2.93 (br d, J=5.1 Hz, 6H), 2.74-2.58 (m, 4H), 2.04-1.94 (m, 1H), 1.73-1.59 (m, 1H); LCMS: [M+H]+=550.62.
Prepared from (S)-4-(4-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.160 mmol, in turn made from 5-bromo-2-morpholinobenzaldehyde and (3S)-3-methoxypyrrolidine hydrochloride) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.209 mmol) to yield the title compound (62 mg, 69% yield). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.6 (m, 1H), 7.5-7.6 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 1H), 7.4-7.4 (m, 1H), 7.1-7.2 (m, 1H), 6.2-6.3 (m, 2H), 3.87 (br s, 1H), 3.75 (br s, 4H), 3.64 (br s, 2H), 3.42 (br s, 2H), 3.14 (br s, 3H), 2.9-3.0 (m, 6H), 2.6-2.8 (m, 4H), 1.9-2.1 (m, 1H), 1.5-1.8 (m, 1H); LCMS: [M+H]+=532.58.
Prepared from (S)-4-(4-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)phenyl)morpholine (60 mg, 0.160 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (84 mg, 0.209 mmol) to yield the title compound as a white solid (42 mg, 45% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.2 (m, 1H), 7.6-7.6 (m, 2H), 7.5-7.6 (m, 1H), 7.4-7.4 (m, 1H), 7.39 (br d, 1H, J=5.7 Hz), 7.1-7.2 (m, 1H), 6.27 (br s, 2H), 3.8-3.9 (m, 1H), 3.75 (br s, 4H), 3.64 (br s, 2H), 3.41 (dt, 2H, J=2.7, 6.4 Hz), 3.14 (br s, 3H), 2.9-3.0 (m, 6H), 2.6-2.7 (m, 2H), 2.47 (br d, 2H, J=8.7 Hz), 1.97 (br d, 1H, J=6.2 Hz), 1.5-1.8 (m, 1H); LCMS: [M+H]+=550.62.
Step 1: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one (100 mg, 0.273 mmol), and 4-piperazinylphenylboronic acid, pinacol ester (102 mg, 0.355 mmol) as a beige solid (111 mg, 91%); LCMS: [M+H]+=448.47.
Step 2: 6-(2-amino-5-(4-(4-(4,4-difluorobutyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-3-methylisoquinolin-1(2H)-one (50 mg, 0.112 mmol) and 4-bromo-1,1-difluorobutane (38.7 mg, 0.223 mmol) to give the title compound was isolated as an off white solid (38 mg, 60%). 1H NMR (500 MHz, METHANOL-d4) δ=7.72-7.66 (m, 1H), 7.51-7.47 (m, 1H), 7.45 (d, J=7.8 Hz, 2H), 7.28-7.20 (m, 1H), 7.08-7.02 (m, 2H), 6.52-6.45 (m, 1H), 6.09-5.81 (m, 1H), 3.29-3.24 (m, 4H), 2.70-2.64 (m, 4H), 2.52-2.46 (m, 2H), 2.36-2.29 (m, 3H), 1.96-1.83 (m, 2H), 1.77-1.68 (m, 2H); LCMS: [M+H]+=540.53.
Step 1: tert-butyl 4-(4-bromo-2-formylphenoxy)piperidine-1-carboxylate
To a mixture of 5-bromosalicylaldehyde (1 g, 4.97 mmol), KI (0.041 g, 0.249 mmol) and K2CO3 (2.063 g, 14.92 mmol) in DMF (10 mL) was added 1-Boc-4-(tosyloxy)piperidine (2.65 g, 7.46 mmol) and the reaction mixture was heated at 90° C. for 2.5 h. The reaction mixture was cooled to room temperature and partitioned between EtOAc and water. The organic layer was separated, washed with I N NaOH (15 mL), water (20 ml), dried over anhydrous Na2SO4, and concentrated to yield the product (1.625 g, 81% yield). LCMS: [M+H]+=328.24 (-tBu)
Step 2: tert-butyl 4-(4-bromo-2-((dimethylamino)methyl)phenoxy)piperidine-1-carboxylate
Prepared using tert-butyl 4-(4-bromo-2-formylphenoxy)piperidine-1-carboxylate (350 mg, 0.911 mmol) and dimethylamine, 2.0M solution in THF (1.822 mL, 3.64 mmol) to give the product as a pale-yellow oil (401 mg, quantitative yield). LCMS: [M+H]+=413.36
Step 3: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenoxy)piperidine-1-carboxylate
Prepared from tert-butyl 4-(4-bromo-2-((dimethylamino)methyl)phenoxy)piperidine-1-carboxylate (60 mg, 0.138 mmol) Example 146, step 2 with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (68.7 mg, 0.179 mmol) to afford the title compound as a beige solid (60 mg, 61% yield). LCMS: [M+H]+=590.61.
Step 4: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperidin-4-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-((dimethylamino)methyl)phenoxy)piperidine-1-carboxylate (60 mg, 0.084 mmol) and TFA (0.579 ml, 7.56 mmol) to give the product was isolated as a yellow solid (42 mg, 97%); LCMS: [M+H]+=490.58.
Step 5: 6-(5-(4-((1-acetylpiperidin-4-yl)oxy)-3-((dimethylamino)methyl)phenyl)-2-amino-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 4 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperidin-4-yloxy)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (42 mg, 0.086 mmol) and Ac20 (8.11 μl, 0.086 mmol) to give the title compound as an off white solid (24 mg, 50%). 1H NMR (500 MHz, METHANOL-d4) δ=8.08-8.02 (m, 1H), 7.71-7.65 (m, 1H), 7.55-7.50 (m, 2H), 7.50-7.45 (m, 2H), 7.14-7.09 (m, 1H), 4.79-4.73 (m, 1H), 3.87-3.77 (m, 2H), 3.65-3.59 (m, 3H), 3.59-3.52 (m, 3H), 3.11-3.05 (m, 2H), 2.35-2.28 (m, 6H), 2.18-2.14 (m, 3H), 2.13-2.05 (m, 1H), 2.04-1.96 (m, 1H), 1.94-1.85 (m, 1H), 1.84-1.77 (m, 1H); LCMS: [M+H]+=532.58.
Prepared from 2 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(piperidin-4-yloxy)phenyl)-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (21 mg, 0.041 mmol) using a set of procedures analogous to the foregoing example to give the title compound as a white solid (15 mg, 63% yield). 1H NMR (500 MHz, METHANOL-d4) δ=7.79-7.73 (m, 1H), 7.70-7.65 (m, 1H), 7.52-7.49 (m, 1H), 7.48-7.44 (m, 1H), 7.44-7.40 (m, 1H), 7.14-7.10 (m, 1H), 4.78-4.73 (m, 1H), 3.87-3.77 (m, 2H), 3.66-3.60 (m, 3H), 3.58-3.53 (m, 3H), 3.07-3.01 (m, 2H), 2.34-2.29 (m, 6H), 2.17-2.14 (m, 3H), 2.12-2.06 (m, 1H), 2.05-1.98 (m, 1H), 1.93-1.86 (m, 1H), 1.84-1.78 (m, 1H); LCMS: [M+H]+=550.62.
Step 1: 6-bromo-4-chloro-2,7-naphthyridin-1(2H)-one
To a solution of 6-bromo-2,7-naphthyridin-1(2H)-one (80 mg, 0.355 mmol) in N,N-dimethylacetamide (2 ml) was added N-chlorosuccinimide (57.0 mg, 0.427 mmol). The mixture was stirred at 50° C. for 1 hour. The reaction was cooled to RT and diluted with water. The white suspension was filtered, washed with water followed by methanol, and dried under vacuum to obtain 6-bromo-4-chloro-2,7-naphthyridin-1(2H)-one (61 mg, 66.1% yield) as a beige solid. LCMS: [M+H]+=259.22.
Step 2: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4-chloro-2,7-naphthyridin-1(2H)-one, hydrochloride
Prepared from 6-bromo-4-chloro-2,7-naphthyridin-1(2H)-one (30 mg, 0.116 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (61.1 mg, 0.139 mmol) to give the free base (4.3 mg) which was taken up in methanol (1 ml) and hydrochloric acid, 4.0 M in dioxane (0.032 ml, 0.127 mmol) to afford the title compound (3.1 mg, 7.5% overall yield) as a bright yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.93 (d, J=6.24 Hz, 1H), 10.34 (br. s., 1H), 9.40 (s, 1H), 8.27 (d, J=10.03 Hz, 1H), 8.02 (s, 1H), 7.77 (d, J=6.24 Hz, 1H), 7.52 (d, J=7.95 Hz, 2 H), 7.10 (d, J=8.80 Hz, 2H), 3.89-3.94 (m, 2H), 3.48-3.55 (m, 4H), 3.14-3.20 (m, 4H), 1.32 (d, J=6.60 Hz, 6H); LCMS: [M+H]+=493.47.
Step 1: (E)-3-(3-bromo-5-fluorophenyl)but-2-enoic acid
To THF (75 mL) at 0° C. under nitrogen atmosphere, sodium hydride (60%, 1.38 g, 34.56 mmol) was add portion wise. Triethyl phosphonoacetate (6.85 mL, 34.56 mmol) was added dropwise and stirred at 0° C. for 20 min. The resulting reaction mass was added dropwise to a solution of 1-(3-bromo-5-fluorophenyl)ethan-1-one (5 g, 23.04 mmol) in THF (50 mL) and the mixture was refluxed for 16 h. The mixture was then diluted with water (200 mL) and extracted with DCM (3×150 mL). The organic layer was dried over Na2SO4 and concentrated under vacuum to afford a yellow oil which was dissolved in MeOH (25 mL) and NaOH (4.6 g, 226.13 mmol) and water (15 mL) were added and the reaction was heated at 50° C. for 2 h. The mixture was concentrated and the resulting aqueous solution was acidified by aqueous 2M HCl and extracted with EtOAc (2×150 mL). The combined organic layers were dried over anhydrous Na2SO4 and concentrated under vacuum to afford the product (3.5 g, 58.6% yield) as white solid. LCMS: [M+H]+=258.9.
Step 2: (E)-3-(3-bromo-5-fluorophenyl)but-2-enoyl chloride
To a cooled solution of (E)-3-(3-bromo-5-fluorophenyl)but-2-enoic acid (3.5 g, 13.58 mmol) in DCM (35 mL) and DMF (0.2 mL) at 0° C., oxalyl chloride (2.05 g, 16.21 mmol) was added dropwise and the reaction was allowed to warm to RT and stirred for 3 h. The mixture was concentrated and azeotroped with toluene (2×20 mL) and DCM (2×20 mL) to give the crude product (3.5 g,) as white solid which was used directly in the next step without analysis.
Step 3: (E)-3-(3-bromo-5-fluorophenyl)but-2-enoyl azide
To a cooled solution of the (E)-3-(3-bromo-5-fluorophenyl)but-2-enoyl chloride (3.5 g, 12.61 mmol) in 1,4 dioxane (35 mL) at 0° C., a suspension of sodium azide (1.47 g, 22.7 mmol) in 1:1 mixture of 1,4-dioxane and water (15 mL) was added and the mixture was gradually warmed to room temperature and stirred for 1.5 h. The mixture was then diluted with water (30 mL) and extracted with diethyl ether (2×100 mL). The combined organic layer was back washed with saturated aqueous NaHCO3 solution (3×100 mL) and water (3×100 mL) and dried over Na2SO4. The organic layer was directly used for next step.
Step 4: 6-bromo-8-fluoro-4-methylisoquinolin-1(2H)-one
To the ether layer of (E)-3-(3-bromo-5-fluorophenyl)but-2-enoyl azide was treated with 1,2 dichlorobenzene (30 mL) and the ether was removed under vacuum to give a solution of (E)-3-(3-bromo-5-fluorophenyl)but-2-enoyl azide in 1,2 dichlorobenzene. The acyl azide solution in 1,2 dichlorobenzene was added dropwise over 30 min to a solution of iodine (0.4 g) in 1,2 dichlorobenzene (30 mL) at 120° C. The mixture was then stirred at 190° C. for 16 h, and allowed to cool room temperature and added to hexane (1000 mL). The suspension was stirred for 1 h and the resulting solid was filtered, washed with EtOAc (50 mL) and DCM (50 mL) and dried under vacuum to give the title compound (0.4 g, 12.7% yield) as pale yellow solid. LCMS: [M+H]+=258.
Step 5: (8-fluoro-4-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-8-fluoro-4-methylisoquinolin-1(2H)-one (400 mg, 1.562 mmol) to give a mixture of the boronic acid and boronate which was used in the next step without further purification. LCMS: [M+H]+ 222.29 boronic acid, 304.42 boronate.
Step 6: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-4-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (200 mg, 0.631 mmol) and (8-fluoro-4-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (167 mg, 0.757 mmol) to give the product (90 mg, 38.9% yield) as a beige solid. LCMS: [M+H]+=366.29.
Step 7: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-8-fluoro-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-4-methylisoquinolin-1(2H)-one (30 mg, 0.082 mmol) and 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (32.5 mg, 0.098 mmol) to give the title compound (15.4 mg, 38.4% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.08 (br. s., 1H), 7.69 (d, J=10.15 Hz, 1H), 7.50 (s, 1H), 7.41 (d, J=7.95 Hz, 2H), 7.36 (d, J=12.35 Hz, 1H), 7.06 (s, 1H), 6.97 (d, J=8.80 Hz, 2H), 6.33 (s, 2H), 3.10-3.18 (m, 4H), 2.63-2.70 (m, 1H), 2.55-2.60 (m, 4H), 2.21 (s, 3H), 1.00 (d, J=6.60 Hz, 6H); LCMS: [M +H]+=490.46.
Step 1: 6-bromo-7-fluoro-3-methylisoquinolin-1(2H)-one
To a RBF was added copper(I) bromide (0.068 g, 0.472 mmol), Cs2CO3 (3.07 g, 9.43 mmol), 2,4-dibromo-5-fluorobenzamide (1.40 g, 4.72 mmol), propan-2-one (1.731 mL, 23.58 mmol) and dimethylsulfoxide (20 ml). The reaction was stirred and heated at 80° C. overnight. The reaction mixture was partitioned between brine (200 ml) and DCM (200 ml). The organic layer was separated, and the aqueous layer washed with DCM (2×100 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated onto celite. The mixture was purified by flash chromatography eluting with 0-10% MeOH/DCM+1% NH4OH. The desired fractions were collected, concentrated and dried under vacuum to afford the product (489 mg, 40.5% yield) as a beige solid. LCMS: [M+H]+=256.21.
Step 2: 6-bromo-4,7-difluoro-3-methylisoquinolin-1(2H)-one
A vial was charged with 6-bromo-7-fluoro-3-methylisoquinolin-1(2H)-one (189 mg, 0.738 mmol) and Selectfluor™ fluorinating reagent (275 mg, 0.775 mmol). Methanol (10 ml) and acetonitrile (10 ml) were added, and the reaction was stirred at room temperature for 5 days. The white suspension was filtered, washed with water, and dried under vacuum to afford the product (158 mg, 78% yield) as a white solid. The material was used in the next step without further purification. LCMS [M+H]+=274.27.
Step 3: (4,7-difluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-4,7-difluoro-3-methylisoquinolin-1(2H)-one (115 mg, 0.420 mmol) to give the boronic acid which was used in the next step without further purification. LCMS: [M+H]+=240.28.
Step 4: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-4,7-difluoro-3-methylisoquinolin-1(2H)-one
Prepared from (4,7-difluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (32.8 mg, 0.137 mmol) and 3-bromo-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-amine (45 mg, 0.114 mmol) to give the title compound (6.2 mg, 10.7% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.36 (br. s., 1H), 7.90 (d, J=9.90 Hz, 1H), 7.73 (d, J=6.72 Hz, 1H), 7.66 (d, J=10.15 Hz, 1H), 7.39 (d, J=8.07 Hz, 2 H), 6.96 (d, J=8.93 Hz, 2H), 6.26 (s, 2H), 3.12-3.17 (m, 4H), 2.64-2.70 (m, 1H), 2.56-2.58 (m, 4H), 2.23 (d, J=2.81 Hz, 3H), 1.00 (d, J=6.48 Hz, 6H); LCMS: [M+H]+=508.57.
Prepared from N-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)-2-methoxy-N-methylethan-1-amine (50 mg, 0.137 mmol, in turn made from bromo-2-(tetrahydro-2H-pyran-4-yl)benzaldehyde and N-(2-methoxyethyl)methylamine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (68.2 mg, 0.178 mmol) to yield the title compound as an off white solid (47 mg, 63% yield). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.91 (d, 1H, J=7.8 Hz), 7.6-7.7 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 2H), 7.4-7.4 (m, 1H), 7.3-7.4 (m, 1H), 6.1-6.4 (m, 2H), 3.9-4.0 (m, 2H), 3.5-3.6 (m, 2H), 3.4-3.5 (m, 4H), 3.4-3.4 (m, 2H), 3.2-3.3 (m, 1H), 3.2-3.2 (m, 3H), 2.9-3.0 (m, 2H), 2.5-2.6 (m, 2H), 2.1-2.2 (m, 3H), 1.7-1.8 (m, 2H), 1.6-1.7 (m, 2H); LCMS: [M+H]+=519.54
Prepared from N-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)benzyl)-2-methoxy-N-methylethan-1-amine (55 mg, 0.151 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (79 mg, 0.196 mmol) to yield the title compound as a white solid (37 mg, 44% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.1 (m, 1H), 7.6-7.7 (m, 2H), 7.4-7.4 (m, 2H), 7.4-7.4 (m, 1H), 7.3-7.4 (m, 1H), 6.2-6.3 (m, 2H), 3.9-4.0 (m, 2H), 3.5-3.6 (m, 2H), 3.4-3.5 (m, 4H), 3.4-3.4 (m, 2H), 3.2-3.3 (m, 1H), 3.2-3.2 (m, 3H), 2.92 (br t, 2H, J=6.4 Hz), 2.5-2.6 (m, 2H), 2.1-2.2 (m, 3H), 1.7-1.7 (m, 2H), 1.6-1.7 (m, 2H); LCMS: [M+H]+=537.59.
Step 1: (R)-5-bromo-2-(2-methylmorpholino)benzaldehyde
A vial containing 5-bromo-2-fluorobenzaldehyde (5 g, 24.63 mmol), (R)-2-methyl-morpholine hydrochloride (3.39 g, 24.63 mmol) and K2CO3 (8.51 g, 61.6 mmol) in dry DMF (25 ml) was heated for 8 h in a in an oil bath at 120° C. The reaction mixture was partitioned between 450 ml water and 100 mL EtOAc, the organic phase was separated, aqueous phase was extracted with EtOAc (3×100 mL), the combined org phase was washed with water (1×250 ml), brine (1×250 ml), dried over Na2SO4 and concentrated. The crude product was purified by silica gel chromatography (eluting with hexanes containing 0-40% EtOAc) to afford the title compound as a pale-yellow oil (5.517 g, 79%). LCMS: [M+H]+=284.36.
Step 2: (R)-1-(5-bromo-2-(2-methylmorpholino)phenyl)-N,N-dimethylmethanamine
Prepared from (R)-5-bromo-2-(2-methylmorpholino)benzaldehyde (1 g, 3.52 mmol) and dimethylamine, 2.0M solution in THF (5.28 ml, 10.56 mmol) to give product as a colorless oil (1.118 g, 96% yield). LCMS: [M+H]+=313.32.
Step 3: (R)-6-(2-amino-5-(3-((dimethylamino)methyl)-4-(2-methylmorpholino)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from (R)-1-(5-bromo-2-(2-methylmorpholino)phenyl)-N,N-dimethylmethanamine (50 mg, 0.160 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (80 mg, 0.208 mmol) to yield the title compound as a white solid (60 mg, 73% yield). 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br s, 1H), 7.9-7.9 (m, 1H), 7.6-7.6 (m, 1H), 7.5-7.5 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 1H), 7.41 (br d, 1H, J=8.4 Hz), 7.1-7.1 (m, 1H), 6.2-6.3 (m, 2H), 3.8-3.9 (m, 1H), 3.7-3.8 (m, 2H), 3.4-3.5 (m, 2H), 3.4-3.4 (m, 2H), 3.1-3.2 (m, 1H), 3.0-3.1 (m, 1H), 2.9-3.0 (m, 2H), 2.7-2.8 (m, 1H), 2.4-2.5 (m, 1H), 2.2-2.2 (m, 6H), 1.1-1.2 (m, 3H); LCMS: [M+H]+=490.52.
Prepared in an analogous manner as the previous example from (R)-1-(5-bromo-2-(2-methylmorpholino)phenyl)-N,N-dimethylmethanamine (50 mg, 0.160 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (83 mg, 0.208 mmol) to yield the title compound as a white solid (24 mg, 28% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.2 (m, 1H), 7.5-7.6 (m, 2H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 1H), 7.4-7.4 (m, 1H), 7.1-7.1 (m, 1H), 6.2-6.3 (m, 2H), 3.8-3.9 (m, 1H), 3.7-3.8 (m, 2H), 3.4-3.5 (m, 2H), 3.4-3.4 (m, 2H), 3.1-3.2 (m, 1H), 3.0-3.1 (m, 1H), 2.9-3.0 (m, 2H), 2.7-2.8 (m, 1H), 2.4-2.5 (m, 1H), 2.1-2.2 (m, 6H), 1.1-1.2 (m, 3H); LCMS: [M+H]+=508.57.
Step 1: (E)-3-(3-bromo-4-fluorophenyl)but-2-enoic acid
To THF (75 mL) at 0° C. under nitrogen atmosphere, sodium hydride (60%, 1.38 g, 34.56 mmol) was added portion wise. Triethyl phosphonoacetate (6.85 mL, 34.56 mmol) was added dropwise and stirred at 0° C. for 20 min and the resulting mixture was added dropwise to a solution of 1-(3-bromo-4-fluorophenyl)ethan-1-one (5 g, 23.04 mmol) in THF (50 mL) and the resulting mixture was refluxed for 16 h. The mixture was then diluted with water (200 mL) and extracted with DCM (3×150 mL). The organic layer was dried over Na2SO4 and concentrated undervacuum to afford a yellow oil. The oil was dissolved in MeOH (25 mL) and NaH (4.6 g, 226.13 mmol) and water (15 mL) were added and the reaction mass was heated at 50° C. for 2 h. The reaction mass was concentrated to evaporate the organics and the resulting aqueous solution was acidified by aqueous 2M HCl and extracted with EtOAc (2×150 mL). The combined organic layers were dried over Na2SO4 and concentrated under vacuum to afford the product (3.5 g, 58.6% yield) as white solid. LCMS: [M+H]+=258.9.
Step 2: (E)-3-(3-bromo-4-fluorophenyl)but-2-enoyl chloride
To a cooled solution of (E)-3-(3-bromo-4-fluorophenyl)but-2-enoic acid (3.5 g, 13.58 mmol) in DCM (35 mL) and DMF (0.2 mL) at 0° C., oxalyl chloride (2.05 g, 16.21 mmol) was added dropwise and the reaction mass was allowed to warm to RT and stirred at same temperature for 3 h. After completion the reaction, reaction mass was concentrated and azeotrope with toluene (2×20 mL) and DCM (2×20 mL) to give the crude product (3.5 g) as white solid which was used as such in the next step without analysis and further purification.
Step 3: (E)-3-(3-bromo-4-fluorophenyl)but-2-enoyl azide
To a cooled solution of crude of (E)-3-(3-bromo-4-fluorophenyl)but-2-enoyl chloride (3.5 g, 12.61 mmol) in 1,4 dioxane (35 mL) at 0° C., a suspension of sodium azide (1.47 g, 22.70 mmol) in 1:1 mixture of 1,4-dioxane and water (15 mL) was added and the reaction mas was gradually warm to room temperature and stirred at same temperature for a 1.5 h. After completion reaction, reaction mass was diluted with water (30 mL) and extracted with diethyl ether (2×100 mL). The combined organic layers were back washed with saturated aq. NaHCO3 (3×100 mL) and water (3×100 mL) and dried over Na2SO4 and organic layer was directly used for next step.
Step 4: 6-bromo-7-fluoro-4-methylisoquinolin-1(2H)-one
The ether layer of (E)-3-(3-bromo-4-fluorophenyl)but-2-enoyl azide was treated with 1,2 dichlorobenzene (30 mL) and the ether was removed under vacuum to give a solution of (E)-3-(3-bromo-4-fluorophenyl)but-2-enoyl azide in 1,2 dichlorobenzene. The acyl azide solution in 1,2 dichlorobenzene was added dropwise over 30 min to a solution of iodine (0.4 g) in 1,2 dichlorobenzene (30 mL) at 120° C. The mixture was then stirred at 190° C. for 16 h, allowed to cool room temperature and added to hexane (1000 mL). The suspension was stirred for 1 h and the resulting solid was washed with EtOAc (50 mL) and DCM (50 mL) and dried under vacuum to give the product (0.8 g, 25.36%) as pale-yellow solid. LCMS: [M+2]+=258.
Step 5: (7-fluoro-4-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-7-fluoro-4-methylisoquinolin-1(2H)-one (800 mg, 3.12 mmol) in a manner analogous to previous examples to give the boronic acid which was used in the next step without further purification. LCMS [M+H]+=222.29.
Step 6: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-4-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (400 mg, 1.262 mmol) and (7-fluoro-4-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (335 mg, 1.515 mmol) to give the product (357 mg, 77% yield) as a beige solid. LCMS [M+H]+=366.36.
Step 7: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-4-methylisoquinolin-1(2H)-one (40 mg, 0.109 mmol) and 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (43.3 mg, 0.131 mmol) to give the title compound (20.2 mg, 37.8% yield) as a grey solid. 1H NMR (500 MHz, DMSO-d6) 0 ppm 11.21 (br. s., 1H), 7.94 (d, J=10.27 Hz, 1H), 7.71 (d, J=6.85 Hz, 1H), 7.66 (d, J=10.03 Hz, 1H), 7.39 (d, J=8.19 Hz, 2H), 7.02 (br. s., 1H), 6.96 (d, J=8.68 Hz, 2H), 6.23 (s, 2H), 3.14 (br. s., 4H), 2.63-2.71 (m, 1H), 2.57 (d, J=4.03 Hz, 4H), 2.23 (s, 3H), 1.00 (d, J=6.48 Hz, 6 H); LCMS [M+H]+=490.40.
Prepared from 4-(2-(azetidin-1-ylmethyl)-6-bromopyridin-3-yl)morpholine (60 mg, 0.173 mmol, in turn made from 6-bromo-3-morpholinopicolinaldehyde and azetidine using procedures analogous to earlier examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (86 mg, 0.225 mmol) to yield the title compound as a white solid (28 mg, 32% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.18-8.07 (m, 1H), 8.02-7.98 (m, 1H), 7.97-7.91 (m, 1H), 7.61-7.55 (m, 1H), 7.58-7.51 (m, 1H), 7.48-7.41 (m, 2H), 6.56-6.39 (m, 2H) 3.80-3.74 (m, 4H), 3.73-3.64 (m, 2H), 3.46-3.40 (m, 2H), 3.39-3.34 (m, 4H), 3.03-2.94 (m, 6H), 2.02-1.93 (m, 2H). LCMS: [M+H]+=489.64.
Prepared from 4-(2-(azetidin-1-ylmethyl)-6-bromopyridin-3-yl)morpholine (60 mg, 0.173 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (90 mg, 0.225 mmol) to yield the title compound as an off white solid (32 mg, 35% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.18-8.07 (m, 2H), 7.68-7.59 (m, 2H), 7.55-7.49 (m, 1H), 7.45-7.38 (m, 1H), 6.56-6.39 (m, 2H), 3.87-3.69 (m, 6H), 3.47-3.39 (m, 4H), 3.39-3.37 (m, 2H), 3.03-2.96 (m, 4H), 2.95-2.91 (m, 2H), 2.13-1.91 (m, 2H); LCMS: [M+H]+=507.57.
Prepared from N-((6-bromo-3-morpholinopyridin-2-yl)methyl)-N-methylethanamine (60 mg, 0.173 mmol, in turn made from 6-bromo-3-morpholinopicolinaldehyde and N-ethylmethylamine analogous to earlier examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (86 mg, 0.223 mmol) to yield the title compound as an off white solid (80 mg, 90% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.0-8.1 (m, 1H), 7.9-8.0 (m, 2H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 1H), 7.4-7.4 (m, 2H), 6.4-6.5 (m, 2H), 3.7-3.8 (m, 4H), 3.6-3.7 (m, 2H), 3.4-3.5 (m, 2H), 3.0-3.1 (m, 4H), 2.9-3.0 (m, 2H), 2.55 (br s, 2H), 2.24 (br s, 3H), 0.9-1.0 (m, 3H); LCMS: [M+H]+=491.59.
Prepared from N-((6-bromo-3-morpholinopyridin-2-yl)methyl)-N-methylethanamine (60 mg, 0.172 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (90 mg, 0.223 mmol) to yield the title compound as a beige solid (68 mg, 74% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.1-8.2 (m, 1H), 8.0-8.1 (m, 1H), 7.6-7.7 (m, 2H), 7.5-7.6 (m, 1H), 7.4-7.4 (m, 1H), 6.4-6.5 (m, 2H), 3.7-3.8 (m, 4H), 3.62 (br s, 2H), 3.4-3.5 (m, 2H), 3.0-3.1 (m, 4H), 2.9-3.0 (m, 2H), 2.5-2.6 (m, 2H), 2.1-2.3 (m, 3H), 0.9-1.0 (m, 3H); LCMS: [M+H]+=509.57.
Prepared from N-((6-bromo-3-morpholinopyridin-2-yl)methyl)-2-methoxy-N-methylethan-1-amine (60 mg, 0.157 mmol, in turn made from 6-bromo-3-morpholinopicolinaldehyde and N-(2-methoxyethyl)methylamine as per previous examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (78 mg, 0.204 mmol) to yield the title compound as an off white solid (45 mg, 52% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.1 (m, 1H), 7.9-8.0 (m, 2H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 1H), 7.4-7.5 (m, 2H), 6.4-6.5 (m, 2H), 3.7-3.8 (m, 4H), 3.69 (s, 2H), 3.4-3.4 (m, 4H), 3.1-3.2 (m, 3H), 3.0-3.1 (m, 4H), 2.9-3.0 (m, 2H), 2.7-2.7 (m, 2H), 2.3-2.3 (m, 3H); LCMS: [M+H]+=521.67.
Prepared from N-((6-bromo-3-morpholinopyridin-2-yl)methyl)-2-methoxy-N-methylethan-1-amine (60 mg, 0.157 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (82 mg, 0.204 mmol) to yield the title compound as a pale-yellow solid (52 mg, 59% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.1-8.2 (m, 1H), 8.1-8.1 (m, 1H), 7.6-7.7 (m, 2H), 7.5-7.6 (m, 1H), 7.4-7.4 (m, 1H), 6.4-6.6 (m, 2H), 3.7-3.8 (m, 4H), 3.68 (br s, 2H), 3.4-3.4 (m, 4H), 3.1-3.2 (m, 3H), 3.0-3.1 (m, 4H), 2.9-3.0 (m, 2H), 2.6-2.7 (m, 2H), 2.2-2.3 (m, 3H); LCMS: [M+H]+=539.66.
Prepared from (S)-4-(6-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)pyridin-3-yl)morpholine and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one to yield the title compound as a white solid (73% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.14-8.07 (m, 1H), 7.99-7.95 (m, 1H), 7.94-7.90 (m, 1H), 7.66-7.61 (m, 1H), 7.55-7.50 (m, 1H), 7.45-7.38 (m, 2H), 6.53-6.32 (m, 2H), 3.86-3.81 (m, 1H), 3.80-3.75 (m, 4H), 3.75-3.65 (m, 2H), 3.44-3.39 (m, 2H), 3.12-3.07 (m, 3H), 3.07-2.99 (m, 4H), 2.96 (br t, J=6.5 Hz, 2H), 2.88-2.79 (m, 1H), 2.75-2.68 (m, 1H), 2.67-2.63 (m, 1H), 2.48-2.45 (m, 1H), 1.97-1.87 (m, 1H), 1.65-1.56 (m, 1H); LCMS: [M+H]+=533.58.
Prepared from (S)-4-(6-bromo-2-((3-methoxypyrrolidin-1-yl)methyl)pyridin-3-yl)morpholine (60 mg, 0.152 mmol) from Example 57, step 1 with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (79 mg, 0.197 mmol) to yield the title compound (77 mg, 88% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.16-8.11 (m, 1H), 8.10-8.05 (m, 1H), 7.65-7.60 (m, 2H), 7.55-7.50 (m, 1H), 7.44-7.39 (m, 1H), 6.53-6.41 (m, 2H), 3.84-3.80 (m, 1H), 3.79-3.75 (m, 4H), 3.74-3.69 (m, 2H), 3.41 (dt, J=2.6, 6.4 Hz, 2H), 3.10-3.07 (m, 3H), 3.06-2.98 (m, 4H), 2.94-2.90 (m, 2H), 2.83-2.78 (m, 1H), 2.71-2.65 (m, 1H), 2.62 (br dd, J=3.1, 10.3 Hz, 1H), 2.57-2.54 (m, 1H), 1.95-1.87 (m, 1H), 1.62-1.54 (m, 1H); LCMS: [M+H]+=551.63.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (50 mg, 0.144 mmol) and 4-(4-tetrahydropyranyl)phenylboronic acid pinacol ester (49.7 mg, 0.172 mmol) to give the title compound (54.2 mg, 88% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.09 (d, J=5.62 Hz, 1H), 8.28 (d, J=8.31 Hz, 1H), 7.69-7.74 (m, 2H), 7.63 (dd, J=8.31, 1.35 Hz, 1H), 7.50 (d, J=7.46 Hz, 2H), 7.31 (d, J=8.31 Hz, 2H), 7.04 (d, J=5.26 Hz, 1H), 6.34 (s, 2H), 3.92-3.98 (m, 2H), 3.44 (td, J=10.97, 3.48 Hz, 2H), 2.79 (tt, J=10.42, 5.23 Hz, 1H), 2.22-2.25 (m, 3H), 1.63-1.74 (m, 4H); LCMS: [M+H]+=430.42.
Step 1: (7-fluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid
Prepared from 6-bromo-7-fluoro-3-methylisoquinolin-1(2H)-one (700 mg, 2.73 mmol) to give the boronic acid which was used in the next step without further purification. LCMS: [M−H]−=220.10.
Step 2: 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 5-bromo-6-fluoro-3-iodopyridin-2-amine (350 mg, 1.104 mmol) and (7-fluoro-3-methyl-1-oxo-1,2-dihydroisoquinolin-6-yl)boronic acid (293 mg, 1.325 mmol) to the product (99 mg, 24.5% yield) as a beige solid. LCMS: [M+H]+=366.29.
Step 3: 6-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoro-3-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3-methylisoquinolin-1(2H)-one (30 mg, 0.082 mmol) and 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (32.5 mg, 0.098 mmol) to give the title compound (15.8 mg, 39.4% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.38 (s, 1H), 7.84 (d, J=10.27 Hz, 1H), 7.65 (d, J=6.97 Hz, 1H), 7.61 (d, J=10.15 Hz, 1H), 7.38 (d, J=8.31 Hz, 2H), 6.96 (d, J=8.68 Hz, 2H), 6.37 (s, 1H), 6.19 (s, 2H), 3.11-3.17 (m, 4H), 2.66 (dd, J=13.27, 6.79 Hz, 1H), 2.55-2.59 (m, 4H), 2.22 (s, 3H), 1.00 (d, J=6.48 Hz, 6H); LCMS: [M+H]+=490.58.
Step 1: 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (53 mg, 0.152 mmol) to give a mixture of the boronate and boronic acid which was used in the next step without further purification. LCMS [M+H]+ 270.38 Boronic acid, Boronate 396.44.
Step 2: 6-(6′-amino-6-((dimethylamino)methyl)-2′-fluoro-5-morpholino-[2,3′-bipyridin]-5′-yl)-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-4-methylisoquinolin-1(2H)-one (59.9 mg, 0.152 mmol) and 1-(6-bromo-3-morpholinopyridin-2-yl)-N,N-dimethylmethanamine (35 mg, 0.117 mmol) to give the title compound (11.6 mg, 20.4% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.11 (d, J=5.38 Hz, 1H), 8.30 (d, J=8.19 Hz, 1H), 8.15 (d, J=10.03 Hz, 1H), 7.69 (s, 1H), 7.65 (d, J=8.19 Hz, 1H), 7.59 (d, J=8.31 Hz, 1H), 7.54 (d, J=8.56 Hz, 1H), 7.05 (d, J=5.50 Hz, 1H), 6.51 (s, 2H), 3.77 (br. s., 4H), 3.52 (s, 2H), 3.05 (br. s., 4H), 2.24 (s, 6H), 2.23 (s, 3H); LCMS: [M+H]+=489.46.
Step 1: 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (53 mg, 0.151 mmol) to give a mixture of the boronate and boronic acid which was used in the next step without further purification. LCMS: [M+H]+ 318.34 Boronic acid, Boronate 400.45.
Step 2: 6-(6′-amino-6-((dimethylamino)methyl)-2′-fluoro-5-morpholino-[2,3′-bipyridin]-5′-yl)-4-fluoroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (60.5 mg, 0.152 mmol) and 1-(6-bromo-3-morpholinopyridin-2-yl)-N,N-dimethylmethanamine (35 mg, 0.117 mmol) to give the title compound (13.6 mg, 23.7% yield) as a beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 11.17 (br. s., 1H), 8.29 (d, J=8.19 Hz, 1H), 8.13 (d, J=9.90 Hz, 1H), 7.77 (s, 1H), 7.68 (d, J=8.19 Hz, 1H), 7.65 (d, J=8.07 Hz, 1H), 7.54 (d, J=8.44 Hz, 1H), 7.43 (br. s., 1H), 6.55 (s, 2H), 3.77 (br. s., 4H), 3.52 (s, 2H), 3.05 (br. s., 4H), 2.24 (s, 6H); LCMS: [M+H]+=493.53.
A microwave vial containing a mixture of XPhos Pd G2 (14.80 mg, 0.019 mmol), tert-butyl 4-(6-chloropyridin-3-yl)piperazine-1-carboxylate (80 mg, 0.269 mmol), 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (124 mg, 0.322 mmol) and K2CO3 (114 mg, 0.537 mmol) in t-butanol (3.5 ml) was flushed with Argon. Water (1. ml) was added and the reaction mixture was heated at 1000C in an oil bath at 100° C. The reaction mixture was concentrated onto celite and purified by silica gel chromatograpy, eluting with dichloromethane containing 0-1.5% MeOH and 0-0.15% NH4OH to afford the title compound as a cream colored solid. (123 mg, 84% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.40-8.34 (m, 1H), 8.12-8.05 (m, 1H), 7.98-7.94 (m, 1H), 7.94-7.90 (m, 1H), 7.66-7.58 (m, 1H), 7.46-7.37 (m, 3H), 6.53-6.26 (m, 2H), 3.56-3.48 (m, 4H), 3.44-3.41 (m, 2H), 3.24-3.16 (m, 4H), 3.00-2.94 (m, 2H), 1.47-1.40 (m, 9H); LCMS: [M+H]+=519.54
Step 1: 6-(6′-amino-2′-fluoro-5-(piperazin-1-yl)-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-9(2H)-one
Prepared by deprotecting tert-butyl 4-(6′-amino-2′-fluoro-5′-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)-[2,3′-bipyridin]-5-yl)piperazine-1-carboxylate (112 mg, 0.216 mmol) with TFA (1.488 ml, 19.44 mmol). The title compound was isolated as a cream colored solid (84 mg, 93% yield); LCMS: [M+H]+=419.45
Step 2: 6-(5-(4-acetylpiperazin-1-yl)-6′-amino-2′-fluoro-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a suspension of 6-(6′-amino-2′-fluoro-5-(piperazin-1-yl)-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.108 mmol) and N,N-Diisopropylethylamine (0.037 ml, 0.215 mmol) in dichloromethane (5 ml) at RT, was added acetic anhydride (10.16 μl, 0.108 mmol) and the reaction mixture was stirred at RT for 60 minutes. The solid was filtered, the filter cake was washed with small volume of DCM (x3) and dried to isolate the title compound as a pale-yellow solid (41 mg, 79% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.3-8.4 (m, 1H), 8.0-8.1 (m, 1H), 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.4-7.5 (m, 3H), 6.3-6.5 (m, 2H), 3.6-3.7 (m, 4H), 3.4-3.4 (m, 2H), 3.2-3.3 (m, 2H), 3.2-3.2 (m, 2H), 2.97 (br t, 2H, J=6.4 Hz), 2.05 (s, 3H); LCMS: [M+H]+=461.44
Step 1: tert-butyl 4-(6′-amino-2′-fluoro-5′-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)-[2,3′-bipyridin]-5-yl)piperazine-1-carboxylate
Prepared from tert-butyl 4-(6-chloropyridin-3-yl)piperazine-1-carboxylate (80 mg, 0.269 mmol) with 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (129 mg, 0.322 mmol) to give the product (54 mg, 22.5% yield). LCMS: [M+H]+=537.53
Step 2: 6-(6′-amino-2′-fluoro-5-(piperazin-1-yl)-[2,3′-bipyridin]-5′-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared by deprotecting tert-butyl 4-(6′-amino-2′-fluoro-5′-(7-fluoro-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)-[2,3′-bipyridin]-5-yl)piperazine-1-carboxylate (54 mg, 0.101 mmol) with TFA (0.694 ml, 9.06 mmol) to give product (34 mg, 53% yield) LCMS: [M +H]+=437.37
Step 3: 6-(5-(4-acetylpiperazin-1-yl)-6′-amino-2′-fluoro-[2,3′-bipyridin]-5′-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(6′-amino-2′-fluoro-5-(piperazin-1-yl)-[2,3′-bipyridin]-5′-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (39 mg, 0.062 mmol) and acetic anhydride (5.83 μl, 0.062 mmol) to afford title compound as an off white solid (23 mg, 74% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.3-8.4 (m, 1H), 8.1-8.2 (m, 1H), 8.0-8.1 (m, 1H), 7.6-7.7 (m, 2H), 7.4-7.5 (m, 2H), 6.3-6.5 (m, 2H), 3.6-3.7 (m, 4H), 3.4-3.5 (m, 2H), 3.2-3.3 (m, 2H), 3.19 (br d, 2H, J=2.6 Hz), 2.94 (br d, 2H, J=4.0 Hz), 2.05 (br d, 3H, J=4.2 Hz); LCMS: [M+H]+=479.49
Prepared from 6-(6′-amino-2′-fluoro-5-(piperazin-1-yl)-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-1(2H)-one (39 mg, 0.093 mmol) and 4-bromo-1,1-difluorobutane (32.2 mg, 0.186 mmol) to give the title compound as a pale-yellow solid (39 mg, 78% yield). 1H NMR (DMSO-d6, 500 MHz) δ 8.3-8.4 (m, 1H), 8.1-8.1 (m, 1H), 7.9-8.0 (m, 2H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 1H), 7.4-7.4 (m, 1H), 7.4-7.4 (m, 1H), 6.3-6.4 (m, 2H), 6.0-6.2 (m, 1H), 3.4-3.5 (m, 2H), 3.2-3.3 (m, 4H), 3.0-3.0 (m, 2H), 2.5-2.5 (m, 4H), 2.3-2.4 (m, 2H), 1.8-1.9 (m, 2H), 1.5-1.7 (m, 2H); LCMS: [M+H]+=511.45.
Prepared from (3-bromobenzyl)dimethylamine (50 mg, 0.234 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.304 mmol) to afford the title compound as a beige solid (43 mg, 45% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.92 (d, J=7.95 Hz, 1H), 7.64 (d, J=10.03 Hz, 1H), 7.42-7.51 (m, 4H), 7.37 (t, J=7.52 Hz, 1H), 7.23 (d, J=7.34 Hz, 1H), 6.19-6.42 (m, 2H), 3.42 (br. s., 2H), 3.38-3.41 (m, 2H), 2.96 (t, J=6.24 Hz, 2H), 2.16 (s, 6H); LCMS: [M+H]+=391.43.
Prepared from 7-(2-amino-5-bromo-6-fluoropyridin-3-yl)-2-methylquinazolin-4(3H)-one (40 mg, 0.115 mmol) and 1-(2-(cis-2,6-dimethylmorpholino)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N,N-dimethylmethanamine hydrochloride (47.1 mg, 0.115 mmol) to give the title compound (41 mg 68.5% yield). 1H NMR (500 MHz, DMSO-d6) δ=12.23 (s, 1H), 8.19-8.06 (m, 1H), 7.66 (s, 1H), 7.64 (d, J=10.0 Hz, 1H), 7.57 (d, J=8.3 Hz, 1H), 7.53 (s, 1H), 7.45 (br d, J=8.8 Hz, 1H), 7.11 (br d, J=8.4 Hz, 1H), 6.30 (s, 2H), 3.82-3.73 (m, 2H), 3.12 (br d, J=11.4 Hz, 2H), 2.39-2.31 (m, 5H), 2.25 (br s, 6H), 1.10 (d, J=6.2 Hz, 6H); LCMS: [M+H]+=517.60.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-8-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.113 mmol) and 1-(2-((2S,6R)-2,6-dimethylmorpholino)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N,N-dimethylmethanamine hydrochloride (46.4 mg, 0.113 mmol) to give the title compound (25.3 mg, 42.4% yield). 1H NMR (500 MHz, DMSO-d6) δ=7.99 (br. s., 1H), 7.63 (d, J=10.03 Hz, 1H), 7.53 (br. s., 1H), 7.43 (br. s., 1H), 7.28 (s, 1H), 7.25 (d, J=12.10 Hz, 1H), 7.12 (br. s, 1H), 6.37 (br. s., 2H), 3.73-3.82 (m, 2H), 3.30-3.55 (m, 4H), 3.10 (d, J=8.44 Hz, 2H), 2.93 (t, J=6.11 Hz, 2H), 2.12-2.44 (m, 8H), 1.11 (d, J=6.11 Hz, 6H); LCMS: [M+H]+=522.55.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.113 mmol) and 1-(2-((2S,6R)-2,6-dimethylmorpholino)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N,N-dimethylmethanamine hydrochloride (46.4 mg, 0.113 mmol) to give the product (22 mg, 37.1% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.12 (br. s., 1H), 7.62 (d, J=9.90 Hz, 2H), 7.53 (br. s., 1H), 7.43 (d, J=6.85 Hz, 2H), 7.15 (br. s., 1H), 6.28 (br. s., 2H), 3.75-3.85 (m, 2H), 3.30-3.55 (m, 4H), 3.09 (br. s., 2H), 2.92 (t, J=6.30 Hz, 2H), 2.12-2.45 (m, 8H), 1.11 (d, J=6.24 Hz, 6H); LCMS: [M+H]+=522.55.
Prepared from (S)-4-(2-(azetidin-1-ylmethyl)-4-bromophenyl)-2-methylmorpholine (50 mg, 0.138 mmol, in turn made from 5-bromo-2-fluorobenzaldehyde and (S)-2-methyl-morpholine through an analogous sequence as earlier examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (68.9 mg, 0.180 mmol) to yield the title compound as an off white solid (35 mg, 48% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.95 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.58 (d, J=10.03 Hz, 1H), 7.44-7.50 (m, 3H), 7.39 (d, J=8.07 Hz, 1H), 7.09 (d, J=8.31 Hz, 1H), 6.25 (s, 2H), 3.86 (d, J=10.88 Hz, 1H), 3.68-3.75 (m, 2H), 3.62 (br. s., 2H), 3.41 (br. s., 2H), 3.12-3.29 (m, 4H), 3.09 (d, J=11.37 Hz, 1H), 3.02 (d, J=11.37 Hz, 1H), 2.96 (t, J=6.48 Hz, 2H), 2.68-2.75 (m, 1H), 2.43 (t, J=10.64 Hz, 1H), 1.99 (t, J=6.72 Hz, 2H), 1.12 (d, J=6.11 Hz, 3H); LCMS: [M+H]+=502.43.
Prepared from (S)-4-(2-(azetidin-1-ylmethyl)-4-bromophenyl)-2-methylmorpholine (50 mg, 0.138 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (72.2 mg, 0.180 mmol) to yield the title compound as an off white solid (20 mg, 26% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.11 (br. s., 1H), 7.60 (dd, J=17.42, 10.09 Hz, 2H), 7.47 (s, 1 H), 7.43 (d, J=6.97 Hz, 1H), 7.36 (d, J=8.19 Hz, 1H), 7.08 (d, J=8.31 Hz, 1H), 6.26 (s, 2H), 3.86 (d, J=10.76 Hz, 1H), 3.68-3.75 (m, 2H), 3.61 (br. s., 2H), 3.41 (br. s., 2H), 3.12-3.26 (m, 4H), 3.09 (d, J=11.37 Hz, 1H), 3.02 (d, J=11.49 Hz, 1H), 2.93 (t, J=6.36 Hz, 2H), 2.67-2.75 (m, 1H), 2.43 (t, J=10.64 Hz, 1H), 1.99 (quin, J=6.69 Hz, 2H), 1.12 (d, J=6.24 Hz, 3 H); LCMS: [M+H]+=520.54.
Step 1: Methyl 6-amino-3-(tetrahydro-2H-pyran-4-yl)picolinate
In an autoclave, 10% Pd/C (50% moist, w/w, 5 g) and methyl 6-amino-3-(3,6-dihydro-2H-pyran-4-yl)picolinate (5 g, 21.34 mmol) were added in EtOAc (50 mL). The reaction mass was stirred at 80° C. with 20 Kg/cm3 pressure of H2 for 36 h. After completion of reaction, reaction mass was filtered through celite bed and the filtrate was concentrated under vacuum to afford product (4.2 g, 83.3% yield) as brown solid used in next step without further purification. LCMS: [M+H]+=237.2.
Step 2: Methyl 6-bromo-3-(tetrahydro-2H-pyran-4-yl)picolinate
To a cooled solution of methyl 6-amino-3-(tetrahydro-2H-pyran-4-yl) picolinate (5 g, 21.18 mmol) in 47% HBr in water (35 mL) at 0° C. Br2 (20.3 g, 126.97 mmol) was added dropwise and the reaction mass was stirred at same temperature for 10 minutes. To this, NaNO2 (8 g, 116.39 mmol) in water (20 mL) was added dropwise at the same temperature and the reaction mass was slowly allowed to warm at room temperature and stirred for 72 h. After completion of reaction, the reaction mass was diluted with water (200 mL) and extracted with EtOAc (3×80 mL). The combined organics was washed with sodium thiosulphate solution (300 mL), dried over Na2SO4, concentrate under vacuum to afford crude. The crude was triturated with diethyl ether to afford the product (4 g, 63% yield) as a yellow solid. LCMS: [M+2]+=302.
Step 3: (6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridin-2-yl)methanol
To a cooled solution of methyl 6-bromo-3-(tetrahydro-2H-pyran-4-yl)picolinate (5.5 g, 18.32 mmol) in dry THF (55 mL) at 0° C., LAH (1 M in THF, 18.3 mL, 18.32 mmol) was added dropwise at 0° C. The reaction mass was stirred at RT for 3 h. After completion of reaction, reaction mass was quenched with the very dil. HCl (50 mL) and extracted with ethyl acetate (3×60 mL). The combined organic was dried over anhydrous sodium sulfate, concentrate under vacuum to afford the product (4.5 g, 90.2%) as pale-yellow liquid. LCMS: [M+2]+=274.1.
Step 4: 6-bromo-3-(tetrahydro-2H-pyran-4-yl)picolinaldehyde
To a cooled solution of (6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridin-2-yl)methanol (4.4 g, 16.16 mmol) in DCM (45 mL) and Dess Martin periodinone (10.3 g, 24.25 mmol) was added at 0° C. portioned wise and the reaction mass was slowly warmed to room temperature and stirred for 16 h. The mixture was diluted with water (150 mL) and extracted with DCM (3×80 mL). The combined organic phase was dried over Na2SO4 and concentrated under vacuum to afford the crude was which purified by column chromatography eluting with 15% EtOAc in hexanes and triturating with hexanes to afford the product (2.3 g, 52.7%) as light yellow. LCMS: [M+2]+=272.
Step 5: 1-(6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridin-2-yl)-N,N-dimethylmethanamine
Prepared from 6-bromo-3-(tetrahydro-2H-pyran-4-yl)picolinaldehyde (135 mg, 0.500 mmol) and dimethylamine, 2.0M solution in THF (1 mL, 1.999 mmol) in a manner similar to previous examples. The title compound was isolated as an off white solid (149 mg, 95% yield, 95% purity). LCMS: [M+H]+=299.34.
Step 6: 6-(6′-amino-6-((dimethylamino)methyl)-2′-fluoro-5-(tetrahydro-2H-pyran-4-yl)-[2,3′-bipyridin]-5′-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 1-(6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridin-2-yl)-N,N-dimethylmethanamine (70 mg, 0.222 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (111 mg, 0.289 mmol) to yield the title compound as a white solid (91.5 mg, 82% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.09 (d, J=9.90 Hz, 1H), 7.96 (br. s., 1H), 7.93 (d, J=7.95 Hz, 1H), 7.78 (d, J=8.19 Hz, 1H), 7.64 (d, J=7.70 Hz, 1H), 7.38-7.45 (m, 2H), 6.46 (br. s., 2H), 3.97 (d, J=10.27 Hz, 2H), 3.59 (s, 2H), 3.40-3.47 (m, 4H), 3.20-3.28 (m, 1H), 2.96 (t, J=6.42 Hz, 2H), 2.16 (s, 6H), 1.63-1.75 (m, 4H); LCMS: [M+H]+=476.42.
Prepared from 1-(6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridin-2-yl)-N,N-dimethylmethanamine (70 mg, 0.222 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (116 mg, 0.289 mmol) to yield the title compound as a white solid (55 mg, 48% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.12 (br. s., 1H), 8.08 (d, J=9.90 Hz, 1H), 7.78 (d, J=8.19 Hz, 1H), 7.58-7.67 (m, 2H), 7.41 (d, J=6.97 Hz, 1H), 6.49 (br. s., 2H), 3.97 (d, J=10.15 Hz, 2H), 3.58 (s, 2H), 3.40-3.48 (m, 4H), 3.25 (t, J=11.31 Hz, 1H), 2.93 (t, J=6.24 Hz, 2H), 2.15, (s, 6H), 1.61-1.74 (m, 4H); LCMS: [M+H]+=494.47.
Prepared from 2-(azetidin-1-ylmethyl)-6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridine (80 mg, 0.231 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (134 mg, 0.334 mmol) to yield the title compound as an off white solid (62 mg, 45% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.07-8.17 (m, 2H), 7.74 (d, J=8.31 Hz, 1H), 7.63 (t, J=9.96 Hz, 2 H), 7.42 (d, J=6.97 Hz, 1H), 6.51 (br. s., 2H), 3.97 (d, J=10.51 Hz, 2H), 3.78 (br. s., 2H), 3.46-3.51 (m, 2H), 3.44 (br. s., 2H), 3.09-3.24 (m, 5H), 2.94 (t, J=6.36 Hz, 2H), 1.90-2.00 (m, 2H), 1.64-1.74 (m, 4H); LCMS: [M+H]+=506.44.
Prepared from N-((6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridin-2-yl)methyl)-N-methylethanamine (70 mg, 0.201 mmol, in turn made from 6-bromo-3-(tetrahydro-2H-pyran-4-yl)picolinaldehyde and N-ethylmethylamine in a manner similar to previous examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (100 mg, 0.261 mmol) to yield the title compound as a white solid (91 mg, 88% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.09 (d, J=9.90 Hz, 1 H), 7.96 (br. s., 1H), 7.93 (d, J=7.82 Hz, 1H), 7.77 (d, J=8.19 Hz, 1H), 7.64 (d, J=7.70 Hz, 1H), 7.37-7.45 (m, 2H), 6.47 (br. s., 2H), 3.98 (d, J=10.15 Hz, 2H), 3.66 (s, 2H), 3.40-3.47 (m, 4H), 3.28 (br. s., 1H), 2.96 (t, J=6.17 Hz, 2H), 2.42 (q, J=6.93 Hz, 2H), 2.08 (s, 3 H), 1.63-1.76 (m, 4H), 1.03 (t, J=6.97 Hz, 3H); LCMS: [M+H]+=490.52.
Prepared from N-((6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridin-2-yl)methyl)-N-methylethanamine (70 mg, 0.201 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (82 mg, 0.205 mmol) to yield the title compound as an off white solid (55 mg, 65% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 8.12 (br. s., 1H), 8.09 (d, J=9.90 Hz, 1H), 7.77 (d, J=8.19 Hz, 1H), 7.60-7.68 (m, 2H), 7.41 (d, J=6.97 Hz, 1H), 6.50 (br. s., 2H), 3.98 (d, J=10.15 Hz, 2 H), 3.65 (s, 2H), 3.38-3.47 (m, 4H), 3.25-3.31 (m, 1H), 2.93 (t, J=6.30 Hz, 2H), 2.41 (q, J=6.72 Hz, 2H), 2.07 (s, 3H), 1.63-1.76 (m, 4H), 1.02 (t, J=7.03 Hz, 3H); LCMS: [M+H]+=508.51.
Prepared from 2-(azetidin-1-ylmethyl)-6-bromo-3-(tetrahydro-2H-pyran-4-yl)pyridine (80 mg, 0.231 mmol, in turn made from 6-bromo-3-(tetrahydro-2H-pyran-4-yl)picolinaldehyde and azetidine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (115 mg, 0.301 mmol) to yield the title compound as a beige solid (41 mg, 35% yield). 1H NMR (500 MHz, DMSO-d6) b 8.1-8.2 (m, 1H), 8.0-8.0 (m, 1H), 7.9-8.0 (m, 1H), 7.7-7.8 (m, 1H), 7.6-7.7 (m, 1H), 7.4-7.5 (m, 2H), 6.3-6.6 (m, 2H), 3.9-4.0 (m, 2H), 3.7-3.8 (m, 2H), 3.4-3.5 (m, 4H), 3.1-3.2 (m, 5H), 2.97 (br t, 2H, J=6.4 Hz), 1.9-2.0 (m, 2H), 1.6-1.8 (m, 4H); LCMS: [M+H]+=488.45.
Prepared from 1-(3-bromo-2-fluoro-6-morpholinophenyl)-N,N-dimethylmethanamine (60 mg, 0.180 mmol, in turn made using a synthetic sequence starting from 2,6-difluorobenzaldehyde and morpholine analogous to previous examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (90 mg, 0.234 mmol) to yield the title compound as a beige solid (87 mg, 93% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.00-7.92 (m, 1H), 7.92-7.87 (m, 1H), 7.54-7.49 (m, 1H), 7.48-7.42 (m, 2H), 7.42-7.34 (m, 1H), 7.07-6.94 (m, 1H), 6.35 (br s, 2H), 3.83-3.73 (m, 4H), 3.58-3.44 (m, 2H), 3.44-3.39 (m, 2H), 3.14-3.03 (m, 4H), 2.97-2.90 (m, 2H), 2.34-2.08 (m, 6H); LCMS: [M+H]+=494.37.
Prepared from 1-(3-bromo-2-fluoro-6-morpholinophenyl)-N,N-dimethylmethanamine (60 mg, 0.180 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (94 mg, 0.234 mmol) yield the title compound as a beige solid (42 mg, 43% yield). 1H NMR (500 MHz, DMSO-d6) δ=8.19-8.04 (m, 1H), 7.61 (d, J=10.1 Hz, 1H), 7.53-7.47 (m, 1H), 7.43-7.38 (m, 1H), 7.37-7.31 (m, 1H), 7.03-6.97 (m, 1H), 6.35 (br s, 2H), 3.76 (br d, J=4.0 Hz, 4H), 3.51-3.46 (m, 2H), 3.40 (br dd, J=4.0, 6.1 Hz, 2H), 3.09 (br s, 4H), 2.92 (br t, J=6.3 Hz, 2H), 2.25-2.18 (m, 6H); LCMS: [M+H]+=512.39.
Prepared from 4-(2-(azetidin-1-ylmethyl)-4-bromo-3-fluorophenyl)morpholine (60 mg, 0.173 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (90 mg, 0.225 mmol) to yield the title compound as a beige solid (45 mg, 47% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm =8.16-8.08 (m, 1H), 7.61 (d, J=10.0 Hz, 1H), 7.52-7.46 (m, 1H), 7.41 (d, J=7.0 Hz, 1H), 7.34-7.25 (m, 1H), 6.97-6.92 (m, 1H), 6.43-6.29 (m, 2H), 3.79-3.72 (m, 4H), 3.58 (br s, 2H), 3.44-3.38 (m, 2H), 3.24-3.18 (m, 4H), 3.06 (br s, 4H), 2.92 (br t, J=6.4 Hz, 2H), 1.93-1.86 (m, 2H); LCMS: [M+H]+=524.43.
Prepared from 4-(2-(azetidin-1-ylmethyl)-4-bromo-3-fluorophenyl)morpholine (60 mg, 0.173 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (86 mg, 0.225 mmol) to yield the title compound as a white solid (30 mg, 33% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.94 (br s, 1H), 7.9-7.9 (m, 1H), 7.50 (br d, 1H, J=9.5 Hz), 7.4-7.5 (m, 2H), 7.3-7.4 (m, 1H), 6.9-7.0 (m, 1H), 6.34 (br s, 2H), 3.75 (br s, 4H), 3.59 (br s, 2H), 3.40 (br s, 2H), 3.21 (br t, 4H, J=6.5 Hz), 3.06 (br s, 4H), 2.95 (br t, 2H, J=6.2 Hz), 1.90 (quin, 2H, J=6.5 Hz); LCMS: [M+H]+=506.38.
Prepared from N-(3-bromo-2-fluoro-6-morpholinobenzyl)-N-methylethanamine (70 mg, 0.173 mmol, in turn made from 3-bromo-2-fluoro-6-morpholinobenzaldehyde and N-ethylmethylamine in a manner analogous to previous examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (86 mg, 0.225 mmol) to yield the title compound as a white solid (27 mg, 29% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.9-8.0 (m, 1H), 7.8-7.9 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 2H), 7.3-7.4 (m, 1H), 7.0-7.0 (m, 1H), 6.3-6.4 (m, 2H), 3.75 (br s, 4H), 3.54 (br s, 2H), 3.40 (br s, 2H), 3.09 (br s, 4H), 2.95 (br t, 2H, J=6.2 Hz), 2.4-2.5 (m, 2H), 2.15 (s, 3H), 1.0-1.1 (m, 3H); LCMS: [M+H]+=508.38.
Prepared from N-(3-bromo-2-fluoro-6-morpholinobenzyl)-N-methylethanamine (70 mg, 0.173 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (90 mg, 0.225 mmol) to yield the title compound as an off white solid (58 mg, 61% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.2 (m, 1H), 7.6-7.6 (m, 1H), 7.5-7.5 (m, 1H), 7.42 (br d, 1H, J=6.8 Hz), 7.3-7.4 (m, 1H), 6.9-7.1 (m, 1H), 6.36 (br s, 2H), 3.75 (br s, 4H), 3.54 (br s, 2H), 3.4-3.4 (m, 2H), 3.0-3.2 (m, 4H), 2.91 (br t, 2H, J=6.0 Hz), 2.47 (br d, 2H, J=6.4 Hz), 2.0-2.2 (m, 3H), 0.9-1.1 (m, 3H); LCMS: [M+H]+=526.50.
Step 1: 5-bromo-2-morpholinonicotinaldehyde
To a solution of 5-bromo-2-fluoropyridine-3-carboxaldehyde (1.0 g, 4.90 mmol) and K2CO3 (1.355 g, 9.80 mmol) in N,N-dimethylformamide (5 ml) was added morpholine (0.444 ml, 5.15 mmol). The mixture was stirred at 80° C. for 2 h then partitioned between water (100 mL) and EtOAc (20 mL). The organic phase was separated, and the aqueous phase was washed with EtOAc (20 mL). The combined organic phases were washed with water, followed by brine, dried over Na2SO4, and concentrated. The crude was purified by flash sgc eluting with 0-30% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum to afford the product (895 mg, 67% yield) as a yellow solid. LCMS [M+H]+=271.10.
Step 2: 1-(5-bromo-2-morpholinopyridin-3-yl)-N,N-dimethylmethanamine
Prepared from 5-bromo-2-morpholinonicotinaldehyde (250 mg, 0.922 mmol) and dimethylamine, 2.0M solution in THF (1.383 mL, 2.77 mmol) to give the product (266 mg, 0.886 mmol, 96% yield) as an off-white solid. LCMS: [M+H]+=300.28.
Step 3: (5-((dimethylamino)methyl)-6-morpholinopyridin-3-yl)boronic acid
Prepared from 1-(5-bromo-2-morpholinopyridin-3-yl)-N,N-dimethylmethanamine (150 mg, 0.500 mmol) to give the boronic acid which was used in the next step without further purification. LCMS [M+H]+=266.37.
Step 4: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-morpholinophenyl)-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.119 mmol) and (5-((dimethylamino)methyl)-6-morpholinopyridin-3-yl)boronic acid (37.9 mg, 0.143 mmol) to give the title compound (29.7 mg, 52.4% yield) as a light grey solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.36 (s, 1H), 7.94 (br. s., 1H), 7.91 (d, J=7.83 Hz, 1H), 7.85 (s, 1H), 7.67 (d, J=10.03 Hz, 1H), 7.47 (d, J=8.19 Hz, 1H), 7.45 (s, 1H), 6.33 (br. s., 2H), 3.71-3.78 (m, 4H), 3.40 (s, 4H), 3.14-3.20 (m, 4H), 2.95 (t, J=6.42 Hz, 2H), 2.19 (s, 6H); LCMS [M+H]+ 477.42.
Prepared from 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (79 mg, 0.206 mmol, prepared from a sequence starting from 2-bromo-5-fluoro-4-formylpyridine and N,N-diisopropylethylamine (1.708 mL, 9.80 mmol) and morpholine) and 1-(2-bromo-5-morpholinopyridin-4-yl)-N,N-dimethylmethanamine (120 mg, 0.172 mmol) to give the title compound (35.7 mg, 43.6% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.35 (s, 1H), 8.11 (d, J=10.03 Hz, 1H), 7.94 (br. s., 1H), 7.92 (d, J=7.95 Hz, 1H), 7.81 (s, 1H), 7.43 (d, J=8.07 Hz, 1H), 7.41 (s, 1H), 6.47 (br. s., 2H), 3.74 (d, J=4.03 Hz, 4H), 3.48 (s, 2H), 3.39-3.45 (m, 2H), 2.94-3.02 (m, 6H), 2.21 (s, 6H); LCMS: [M+H]+=477.42.
Prepared from N-(5-bromo-2-morpholinobenzyl)-N-methylpropan-2-amine (40 mg, 0.122 mmol, in turn prepared using a sequence starting from 2-bromo-5-fluoro-4-formylpyridine and morpholine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (60.9 mg, 0.159 mmol) to yield the title compound as a white solid (23 mg, 36% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.0-8.1 (m, 1H), 7.9-8.0 (m, 1H), 7.6-7.7 (m, 2H), 7.5-7.5 (m, 2H), 7.45 (br d, 1H, J=7.8 Hz), 7.19 (br d, 1H, J=8.2 Hz), 6.31 (br s, 2H), 3.8-3.8 (m, 4H), 3.63 (br s, 2H), 3.5-3.5 (m, 2H), 3.0-3.0 (m, 6H), 2.9-2.9 (m, 1H), 2.1-2.2 (m, 3H), 1.0-1.1 (m, 6H); LCMS: [M+H]+=504.44.
Prepared from N-(5-bromo-2-morpholinobenzyl)-N-ethylethanamine (45 mg, 0.138 mmol, prepared from a sequence starting from 5-bromo-2-morpholinobenzaldehyde and diethylamine, analogous to previous examples) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (68.5 mg, 0.179 mmol) to yield the title compound as a beige solid (37 mg, 51% yield). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.63 (br s, 1H), 7.58 (br d, 1H, J=10.0 Hz), 7.4-7.5 (m, 2H), 7.3-7.4 (m, 1H), 7.1-7.2 (m, 1H), 6.2-6.3 (m, 2H), 3.73 (br s, 4H), 3.60 (br s, 2H), 3.52 (br s, 2H), 2.9-3.0 (m, 2H), 2.89 (br s, 4H), 2.4-2.5 (m, 4H), 0.9-1.0 (m, 6H); LCMS: [M+H]+=504.44.
Prepared from N-(5-bromo-2-morpholinobenzyl)-N-methyl-1-(tetrahydrofuran-2-yl)methanamine (35 mg, 0.095 mmol, prepared from a sequence starting from 5-bromo-2-morpholinobenzaldehyde and methyl(oxaolan-2-ylmethyl)amine) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (47.2 mg, 0.123 mmol) to yield the title compound as a white solid (22.5 mg, 41% yield). 1H NMR (500 MHz, DMSO-d6) δ=7.98-7.93 (m, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.65-7.58 (m, 2H), 7.48-7.45 (m, 1H), 7.44 (s, 1H), 7.43-7.39 (m, 1H), 7.16-7.11 (m, 1H), 6.32-6.19 (m, 2H), 3.98-3.90 (m, 1H), 3.79-3.72 (m, 4H), 3.65 (q, J=7.2 Hz, 1H), 3.62-3.56 (m, 2H), 3.56-3.51 (m, 1H), 3.45-3.38 (m, 2H), 2.99-2.89 (m, 6H), 2.47-2.38 (m, 2H), 2.28-2.20 (m, 3H), 1.93-1.84 (m, 1H), 1.77-1.69 (m, 2H), 1.49-1.39 (m, 1H); LCMS: [M+H]+=546.43.
Prepared from 1-(5-bromo-2-fluorophenyl)-N,N-dimethylmethanamine (60 mg, 0.230 mmol, in turn prepared from 5-bromo-2-fluorobenzaldehyde and Dimethylamine, 2.0M solution in THF as per previous procedures) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (115 mg, 0.299 mmol) to afford the title compound as a white solid (45 mg, 46% yield). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.7 (m, 1H), 7.5-7.6 (m, 1H), 7.4-7.5 (m, 3H), 7.2-7.2 (m, 1H), 6.33 (s, 2H), 3.5-3.5 (m, 2H), 3.4-3.4 (m, 2H), 2.9-3.0 (m, 2H), 2.1-2.2 (m, 6H); LCMS: [M+H]+=409.35.
Prepared from 1-(5-bromo-2-fluorophenyl)-N,N-dimethylmethanamine (60 mg, 0.230 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (120 mg, 0.299 mmol) to afford the title compound as a white solid (23.5 mg, 23% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.1-8.2 (m, 1H), 7.6-7.7 (m, 2H), 7.5-7.6 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 1H), 7.2-7.3 (m, 1H), 6.3-6.4 (m, 2H), 3.5-3.5 (m, 2H), 3.4-3.4 (m, 2H), 2.9-3.0 (m, 2H), 2.1-2.2 (m, 6H). LCMS: [M+H]+=427.28.
Prepared from N-(5-bromo-2-morpholinobenzyl)-N-methyl-1-(tetrahydrofuran-2-yl)methanamine (35 mg, 0.095 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (49.4 mg, 0.123 mmol) to yield the title compound as a white solid (7.6 mg, 13% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.0-8.1 (m, 1H), 7.5-7.6 (m, 3H), 7.33 (br dd, 2H, J=7.4, 16.8 Hz), 7.0-7.1 (m, 1H), 6.0-6.3 (m, 2H), 3.8-3.9 (m, 1H), 3.6-3.7 (m, 4H), 3.5-3.6 (m, 1H), 3.4-3.5 (m, 3H), 3.3-3.4 (m, 2H), 2.8-2.9 (m, 6H), 2.35 (br d, 2H, J=6.2 Hz), 2.16 (br s, 3H), 1.7-1.8 (m, 1H), 1.6-1.7 (m, 2H), 1.3-1.4 (m, 1H); LCMS: [M+H]+=564.41.
Step 1: 3-(prop-2-yn-1-yloxy)prop-1-ene
60% NaH in mineral oil (30 g, 750 mmol) was washed with hexanes (3×200 mL) and diethyl ether (100 mL) under nitrogen atmosphere and diethyl ether (500 mL) was added to it; followed by dropwise addition of prop-2-en-1-ol (36 g, 620 mmol) (vigorous gas evolution and reaction mass thickening was observed). After Completion of the addition, the mixture was heated at 30° C. for 1 h and propargyl bromide (178 g, 750 mmol) was added dropwise at RT. The reaction mass was stirred for 16 h at 35-40° C. After 16 h, the mixture was cooled to RT and quenched with water (250 mL) and layers were separated. The organic phase was used as such for the next step without any analysis.
Step 2: 2-(3-(allyloxy)prop-1-en-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane
To a diethyl ether solution of 3-(prop-2-yn-1-yloxy)prop-1-ene (48 g, 500 mmol) in toluene (1.5 L) was added CuCl (4.95 g, 50 mmol), bis(pinacolato)diboron (139 g, 550 mmol), (t-Bu)3P.HBF4 (17.46 g, 60 mmol) and t-BuONa (14.4 g, 150 mmol) at RT under nitrogen atmosphere. After 5 min, methanol (32 mL, 1000 mmol) was dropwise added at RT and the reaction mass was heated up to 80° C. and allowed to stir at RT for 16 h. The mixture was passed through silica and eluted in hexane to afford the product (15 g, 13.4%) as a light yellow to green liquid. 1H NMR (400 MHz, CDCl3) δ 5.99-5.92 (t, J=13.6 Hz, 2H), 5.30 (d, J=12.4 Hz, 1H), 5.18 (d, J=14 Hz, 1H), 4.09 (s, 2H), 4.03 (d, J=5.6 Hz, 1H), 1.29 (s, 12H)
Step 3: 2-(2,5-dihydrofuran-3-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane
A solution 2-(3-(allyloxy)prop-1-en-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (15 g, 6.69 mmol) in DCM (1.5 L) was heated at 50° C. for 1 h under nitrogen atmosphere. After 1 h, the reaction mass was cooled to room temperature and 2nd Generation Grubss catalyst (2.1 g, 3.35 mmol), was added to and the reaction mass was stirred at same temperature for 16 h. After 16 h, the reaction mass was evaporated and purified by column purification and product was eluted 10% ethyl acetate in hexanes to afford the product (3.4 g, 25.9% yield) as a yellow liquid. 1H NMR (400 MHz, CDCl3) δ 6.58 (s, 1H), 4.78-4.77 (m, 2H), 4.72-4.70 (m, 2H), 1.30 (s, 15H).
Step 4: methyl 2-(2,5-dihydrofuran-3-yl)-5-nitrobenzoate
To a degassed solution of methyl 2-iodo-5-nitrobenzoate (25 g, 96.1 mmol), 2-(2,5-dihydrofuran-3-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (19 g, 96.1 mmol, impure) and Na2CO3 (3.6 g, 288.4 mmol) in dioxane: water (2: 1, 370 mL) was added Pd(dppf)Cl2.DCM (7.85 g, 9.61 mmol) and the reaction mass was stirred at 90° C. for 3 h. The mixture was then diluted with water (400 mL) and extracted with EtOAc (3×300 mL). The crude was purified by column chromatography and the product was eluted in 20% EtOAc in hexanes to afford the product (8 g, 39.4% yield) as light yellow semi-solid. 1H NMR (400 MHz, Chloroform-d) δ 8.68 (d, J=2 Hz, 1H), 8.47 (s, 1H), 8.33 (dd, J=8.4, 2.4 Hz, 1H), 7.50 (d, J=8.4 Hz, 1H), 6.02 (s, 1H), 4.90 (s, 4H), 3.96 (s, 3H).
Step 5: Methyl 5-amino-2-(tetrahydrofuran-3-yl)benzoate
In a 3 neck RBF, 10% Pd/C (50% moist, 8 g) and methyl 2-(2,5-dihydrofuran-3-yl)-5-nitrobenzoate (8 g, 32.08 mmol) were added in EtOAc (400 mL) and MeOH (8 mL). The reaction mass was stirred at 80° C. with 20 Kg/cm2 pressure of hydrogen for 16 h. The mixture was then filtered through celite bed and the filtrate was concentrated under vacuum to afford the product (6.5 g, 31.5% yield) as an yellow semi solid. LCMS: [M+H]+=222.1.
Step 6: Methyl 5-bromo-2-(tetrahydrofuran-3-yl)benzoate
To a solution of methyl 5-amino-2-(tetrahydrofuran-3-yl)benzoate (6 g, 27.1 mmol) in bromoform (18 mL) was dropwise added tert-butylnitrite (12 mL) at room temperature and the reaction mass was stirred for 30 min. The crude product was purified by column chromatography and the product was eluted in 10% EtOAc in hexanes to afford the product (3 g, 38.8% yield) as a yellow semi-solid. LCMS: [M+2]+=287.1.
Step 7: 5-bromo-2-(tetrahydrofuran-3-yl)benzoate
To a cooled solution of methyl 5-bromo-2-(tetrahydrofuran-3-yl)benzoate (3.5 g, 12.28 mmol) in dry THF (70 mL) was added LAH (1 M in THF, 18.42 mL, 18.42 mmol) dropwise at 0° C. and the reaction mass was stirred at RT for 3 h. The mixture was quenched with the dil. HCl (50 mL) and extracted with EtOAc (3×100 mL). The combined organics were dried over Na2SO4, and concentrated under vacuum to afford the product (2.5 g, 79.21%) as pale yellow liquid. LCMS: [M-18]+2=241.1.
Step 8: 5-bromo-2-(tetrahydrofuran-3-yl)benzaldehyde
To a solution of methyl 5-bromo-2-(tetrahydrofuran-3-yl)benzoate (2.5 g, 9.72 mmol) in DCM (50 mL) was added MnO2 (8.5 g, 97.2 mmol) at room temperature and the reaction mass was stirred at room temperature for 48 h. After completion of reaction, reaction mass was filtered through celite bed and the filtrate was concentrate under vacuum to afford crude product. The crude was purified by column chromatography and the product was eluted in 5-10% EtOAc in hexanes to afford the product(1 g, 41% yield) as light yellow semi-solid. LCMS: [M+2]=257.2.
Step 9: 1-(5-bromo-2-(tetrahydrofuran-3-yl)phenyl)-N,N-dimethylmethanamine
Prepared from 5-bromo-2-(tetrahydrofuran-3-yl)benzaldehyde (155 mg, 0.608 mmol) and dimethylamine, 2.0M solution in THF (1.215 ml, 2.430 mmol) to give the product as a pale-yellow oil (147 mg, 81% yield). LCMS: [M+H]+=284.30 Step 10: 6-(2-amino-5-(3-((dimethylamino)methyl)-4-(tetrahydrofuran-3-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one)
Prepared from 1-(5-bromo-2-(tetrahydrofuran-3-yl)phenyl)-N,N-dimethylmethanamine (54 mg, 0.181 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (90 mg, 0.235 mmol) to yield the title compound as an off white solid (44.5 mg, 51% yield). 1H NMR (500 MHz, DMSO-d6) δ 7.9-8.0 (m, 1H), 7.9-7.9 (m, 1H), 7.6-7.6 (m, 1H), 7.4-7.5 (m, 3H), 7.3-7.4 (m, 2H), 6.1-6.4 (m, 2H), 3.9-4.0 (m, 2H), 3.8-3.9 (m, 2H), 3.5-3.6 (m, 1H), 3.5-3.5 (m, 1H), 3.4-3.4 (m, 3H), 2.9-3.0 (m, 2H), 2.3-2.3 (m, 1H), 2.15 (s, 6H), 1.9-2.0 (m, 1H); LCMS: [M+H]+=461.44.
Prepared from 1-(5-bromo-2-(tetrahydrofuran-3-yl)phenyl)-N,N-dimethylmethanamine (50 mg, 0.176 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (92 mg, 0.229 mmol) to yield the title compound as an off white solid (16 mg, 18% yield). 1H NMR (500 MHz, DMSO-d6) δ 8.0-8.2 (m, 1H), 7.6-7.7 (m, 2H), 7.4-7.5 (m, 2H), 7.3-7.4 (m, 2H), 6.2-6.4 (m, 2H), 3.9-4.0 (m, 2H), 3.8-3.9 (m, 2H), 3.5-3.6 (m, 1H), 3.5-3.5 (m, 1H), 3.4-3.4 (m, 3H), 2.93 (br t, 2H, J=6.3 Hz), 2.3-2.3 (m, 1H), 2.1-2.2 (m, 6H), 1.9-2.0 (m, 1H); LCMS: [M+H]+=479.43.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.119 mmol) and (5-((dimethylamino)methyl)-2-fluoro-4-morpholinophenyl)boronic acid (40.3 mg, 0.143 mmol, prepared starting from 5-bromo-2,4-difluorobenzaldehyde and morpholine using a sequence analogous to that described earlier) to give the title compound (6.7 mg, 11% yield) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (br. s., 1H), 7.90 (d, J=7.83 Hz, 1H), 7.49 (d, J=9.54 Hz, 1H), 7.36-7.46 (m, 3H), 6.97 (d, J=12.10 Hz, 1H), 6.34 (br. s., 2H), 3.74 (br. s., 4H), 3.39 (br. s., 4H), 2.91-3.01 (m, 6H), 2.16 (s, 6H); LCMS: [M+H]+=494.41.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (35 mg, 0.104 mmol) and (5-((dimethylamino)methyl)-2,3-difluoro-4-morpholinophenyl)boronic acid (37.5 mg, 0.125 mmol, prepared starting from 5-bromo-2,3,4-trifluorobenzaldehyde and morpholine using methods described previously) to give the title compound (10.7 mg, 20% yield) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br. s., 1H), 7.90 (d, J=7.95 Hz, 1H), 7.54 (d, J=9.54 Hz, 1H), 7.41-7.47 (m, 2H), 7.23 (d, J=7.70 Hz, 1H), 6.47 (br. s., 2H), 3.71 (br. s., 4H), 3.46 (s, 2H), 3.39 (td, J=3.18, 6.36 Hz, 2H), 3.09 (br. s., 4H), 2.94 (t, J=6.48 Hz, 2H), 2.16 (s, 6H); LCMS: [M+H]+=512.33.
Step 1: 4-bromo-2-(1-(dimethylamino)ethyl)phenol
Prepared using procedures similar to earlier examples from 5′-bromo-2′-hydroxyacetophenone 98% (1000 mg, 4.65 mmol) and dimethylamine, 2.0M solution in THF (9.30 ml, 18.60 mmol) to give the product (684 mg, 88% purity). LCMS: [M+H]+=244.29.
Step 2: 6-(2-amino-5-(3-(1-(dimethylamino)ethyl)-4-hydroxyphenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 4-bromo-2-(1-(dimethylamino)ethyl)phenol (50 mg, 0.205 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (102 mg, 0.266 mmol) to afford the title compound as an off white solid (62 mg, 68% yield). 1H NMR (500 MHz, DMSO-d6) δ 12.60 (s, 1H), 7.9-8.0 (m, 1H), 7.91 (d, 1H, J=7.9 Hz), 7.6-7.6 (m, 1H), 7.5-7.5 (m, 1H), 7.4-7.5 (m, 1H), 7.3-7.3 (m, 1H), 7.2-7.2 (m, 1H), 6.7-6.8 (m, 1H), 6.1-6.2 (m, 2H), 3.6-3.7 (m, 1H), 3.4-3.4 (m, 2H), 2.9-3.0 (m, 2H), 2.2-2.3 (m, 6H), 1.3-1.3 (m, 3H); LCMS: [M+H]+=421.40.
Step 1: 2-(5-bromo-2-fluorophenyl)propan-2-o
To a 0° C. solution of methyl 5-bromo-2-fluorobenzoate (6.0 g, 25.7 mmol) in anhydrous tetrahydrofuran (50 ml) was added MeMgCl, 3M in THF (21.46 ml, 64.4 mmol) dropwise. The reaction was warmed to room temperature and stirred overnight. The solution was poured into an aqueous saturated solution of ammonium chloride and the organic material was extracted with EtOAc (2×). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated. The crude material was carried onto the next step without further characterization.
Step 2: N-(2-(5-bromo-2-fluorophenyl)propan-2-yl)-2-chloroacetamide
To a solution of 2-(5-bromo-2-fluorophenyl)propan-2-ol (6.00 g, 25.7 mmol) in chloroacetonitrile 99% (48.9 mL, 772 mmol) was added acetic acid (4.42 mL, 77 mmol). The reaction was cooled to 0° C. and sulfuric acid (4.41 mL, 82 mmol) was added dropwise. The reaction was warmed to room temperature and stirred overnight. The mixture was then poured into ice and extracted with EtOAc. The organic layer was washed with aqueous 1N NaOH(aq) solution and brine, dried over anhydrous Na2SO4, and concentrated to give the crude product (7.94 g, 100%) which was carried onto the next step. LCMS: [M+H]+=308.03.
Step 3: 2-(5-bromo-2-fluorophenyl)propan-2-amine
To a solution of N-(2-(5-bromo-2-fluorophenyl)propan-2-yl)-2-chloroacetamide (5.0 g, 16.20 mmol) in ethanol (30 mL) was added concentrated HCl (9.91 ml, 405 mmol) and refluxed overnight. The reaction was concentrated in vacuo and partitioned between DCM and 1 N NaOH(aq). The organic layer was removed, and the aqueous layer was further washed with DCM (2x). The organic layers were combined, dried over Na2SO4, concentrated, and dried under vacuum to obtain the product (1.54 g, 41% yield) as a yellow oil. LCMS: [M+H]+=232.11.
Step 4: (3-(2-aminopropan-2-yl)-4-fluorophenyl)boronic acid
Prepared from 2-(5-bromo-2-fluorophenyl)propan-2-amine (200 mg, 0.862 mmol) to give the boronic acid which was used in the next step without further purification. LCMS: [M+H]+=198.21.
Step 5: 6-(2-amino-5-(3-(2-aminopropan-2-yl)-4-fluorophenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.119 mmol) and (3-(2-aminopropan-2-yl)-4-fluorophenyl)boronic acid (70.3 mg, 0.357 mmol) to give the title compound (31.5 mg, 64.8% yield) as white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.95 (br. s., 1H), 7.91 (d, J=7.95 Hz, 1H), 7.73 (dd, J=1.90, 8.13 Hz, 1H), 7.68 (d, J=10.15 Hz, 1H), 7.46 (d, J=7.95 Hz, 1H), 7.43 (s, 1H), 7.41 (dt, J=2.26, 4.13 Hz, 1H), 7.14 (dd, J=8.44, 12.23 Hz, 1H), 6.29 (s, 2H), 3.40 (dt, J=2.69, 6.42 Hz, 2H), 2.95 (t, J=6.48 Hz, 2H), 2.30 (br. s., 2H), 1.43 (s, 6H); LCMS: [M +H]+=409.29.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (50.1 mg, 0.144 mmol) and 1-(2-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N,N-dimethylmethanamine (58 mg, 187 mmol, prepared from 1-(5-bromo-2-fluorophenyl)-N,N-dimethylmethanamine) to afford the title compound as an off white solid (34 mg, 53% yield). 1H NMR (500 MHz, DMSO-d6)=11.16-11.06 (m, 1H), 8.33-8.24 (m, 1H), 7.76-7.72 (m, 1H), 7.72-7.69 (m, 1H), 7.65-7.61 (m, 1H), 7.57-7.53 (m, 1H), 7.53-7.47 (m, 1H), 7.27-7.19 (m, 1H), 7.09-7.01 (m, 1H), 6.47-6.34 (m, 2H), 3.50-3.44 (m, 2H), 2.28-2.23 (m, 3H), 2.20-2.13 (m, 6H); LCMS: [M+H]+=421.28.
Prepared from N,N-dimethyl-1-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)methanamine (55 mg, 0.190 mmol) and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (50.8 mg, 0.146 mmol) to afford the title compound as a beige solid (31.5 mg, 51% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.09 (br d, J=5.3 Hz, 1H), 8.34-8.24 (m, 1H), 7.78-7.69 (m, 2H), 7.66-7.58 (m, 1H), 7.48-7.42 (m, 2H), 7.40-7.32 (m, 1H), 7.25-7.19 (m, 1H), 7.07-7.01 (m, 1H), 6.44-6.30 (m, 2H), 3.47-3.39 (m, 2H), 2.29-2.22 (m, 3H), 2.19-2.13 (m, 6H); LCMS: [M+H]+=403.41.
Step 1 (S,E)-N-(6-bromochroman-4-ylidene)-2-methylpropane-2-sulfinamide
6-Bromo-2,3-dihydro-4h-chromen-4-one (5 g, 22.02 mmol) was mixed with (S)-(−)-2-Methyl-2-propanesulfinamide (6.67 g, 55.1 mmol) in Toluene (50 ml), then titanium(IV) ethoxide (5.54 ml, 26.4 mmol) was added. The reaction mixture was stirred at 90° C. for 2 hrs and quenched with ice-water and EtOAc. a lot of solid came out and filtered. The solid was rinsed with EtOAc several time. The organic layer of the filtrate was dried with MgSO4, concentrated with silica gel purified by column chromatography, eluted with 0-30% EtOAc in hexanes to give the product (6.697 g, 92% yield) as a yellow solid. LCMS: [M+H]+=330.19.
Step 2: (S)—N—((S)-6-bromochroman-4-yl)-2-methylpropane-2-sulfinamide and (S)—N—((R)-6-bromochroman-4-yl)-2-methylpropane-2-sulfinamide
(S,E)-N-(6-bromochroman-4-ylidene)-2-methylpropane-2-sulfinamide (3.3 g, 9.99 mmol) was mixed with THF (40 mL) and water (0.6 ml), then was cooled with dry ice-acetone bath (<−50° C.). Sodium borohydride (1.134 g, 30.0 mmol) was added and kept stirring 30 min, then slowly warmed to r.t. for 2 hrs. The reaction mixture quenched with sat-NH4Cl and extracted with EtOAc. The organic layer was washed with brine, concentrated with silica gel. Purification column chromatography. eluted with 20-25% EtOAc in hexanes to give the product (2.557 g, 77% yield) 890 mg as a white solid. LCMS: [M+H]+=332.06.
Step 3: (R)-6-bromochroman-4-amine hydrochloride
(R)-6-bromochroman-4-amine hydrochloride (395 mg, 117% yield) was prepared from (S)—N—((R)-6-bromochroman-4-yl)-2-methylpropane-2-sulfinamide (423 mg, 1.273 mmol) reacted with 4.0 M hydrochloric acid in dioxane (3.18 mL, 12.73 mmol) in methanol (5 mL) at r.t. for 90 min. LCMS: [M−HCl—NH3+H]+=211.18.
Step 4: (R)-6-bromo-N,N-dimethylchroman-4-amine
Prepared from (R)-6-bromochroman-4-amine hydrochloride (395 mg, 1.493 mmol) reacted with 37% formaldehyde solution in water (485 mg, 5.97 mmol), sodium acetate (122 mg, 1.493 mmol) and sodium triacetoxyborohydride (949 mg, 4.48 mmol) in methanol (2 ml) and dichloromethane (DCM) (8 ml) at r.t. For 1 h to give the product (374 mg, 98% yield). [M+H]+=256.20.
Step 5: (R)—N,N-dimethyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)chroman-4-amine hydrochloride
Prepared from (R)-6-bromo-N,N-dimethylchroman-4-amine to give the product (87% yield). LCMS=[M−HCl+H]+=304.36.
Step 6: (R)-6-(2-amino-5-(4-(dimethylamino)chroman-6-yl)-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (35.9 mg, 0.103 mmol) to give the title compound (23.5 mg, 51.0% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.09 (br d, J=5.3 Hz, 1H), 8.28 (d, J=8.2 Hz, 1H), 7.72 (s, 1H), 7.66-7.59 (m, 2H), 7.55 (s, 1H), 7.31 (br d, J=8.6 Hz, 1H), 7.04 (s, 1H), 6.79 (d, J=8.4 Hz, 1H), 6.27 (s, 2H), 4.39-4.28 (m, 1H), 4.16-4.06 (m, 1H), 3.80 (dd, J=5.6, 8.3 Hz, 1H), 2.24 (s, 3H), 2.21 (s, 6H), 2.02-1.87 (m, 2H); [M+H]+=445.35.
Step 1: (S)-6-(2-amino-5-(3-(dimethylamino)-2,3-dihydrobenzofuran-5-yl)-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
In a similar manner, (S)-5-bromo-N,N-dimethyl-2,3-dihydrobenzofuran-3-amine (0.99 g, 93% yield) was prepared from (S)-5-Bromo-2,3-dihydro-benzofuran-3-ylamine hydrochloride (1.1012 g, 4.40 mmol) and 37% formaldehyde solution in water (1.427 g, 17.58 mmol), sodium acetate (0.361 g, 4.40 mmol) and sodium triacetoxyborohydride (2.79 g, 13.19 mmol) in dichloromethane (20 mL) and methanol (5 mL) at r.t. for 30 min to give the product (0.99 g, 93% yield). LCMS: [M+H]+=242.23
Step 2: (S)—N,N-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,3-dihydrobenzofuran-3-amine
Prepared from (S)-5-bromo-N,N-dimethyl-2,3-dihydrobenzofuran-3-amine (0.99 g, 4.09 mmol) and bis(pinacolato)diboron (1.246 g, 4.91 mmol), potassium acetate (1.204 g, 12.27 mmol), and [1,1′-bis(diphenylphosphino)ferrocene] dichloropalladium(II) (0.299 g, 0.409 mmol) in 1,4-dioxane (20 mL) at 90-95° C. overnight to give the product (0.94 g, 79% yield). LCMS: [M+H]+=290.31
Step 3: (S)-6-(2-amino-5-(3-(dimethylamino)-2,3-dihydrobenzofuran-5-yl)-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (42.1 mg, 0.121 mmol) and (S)—N,N-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,3-dihydrobenzofuran-3-amine (35 mg, 0.121 mmol) to give the title compound (21.7 mg, 42% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.09 (br d, J=5.5 Hz, 1H), 8.28 (d, J=8.3 Hz, 1H), 7.74 (s, 1H), 7.67 (d, J=10.3 Hz, 1H), 7.65-7.61 (m, 1H), 7.48 (s, 1H), 7.40 (d, J=8.4 Hz, 1H), 7.08-7.02 (m, 1H), 6.86 (d, J=8.3 Hz, 1H), 6.28 (s, 2H), 4.54 (dd, J=3.1, 9.9 Hz, 1H), 4.50-4.44 (m, 1H), 4.42-4.34 (m, 1H), 2.24 (s, 3H), 2.12 (s, 6H). LCMS: [M+H]+=431.43.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (37.8 mg, 0.107 mmol) and (S)—N,N-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,3-dihydrobenzofuran-3-amine hydrochloride (35 mg, 0.107 mmol) to give the title compound (21.5 mg, 46.0% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.17 (br s, 1H), 8.27 (dd, J=1.7, 8.3 Hz, 1H), 7.84 (d, J=1.1 Hz, 1H), 7.75 (dd, J=1.5, 8.3 Hz, 1H), 7.69 (d, J=10.1 Hz, 1H), 7.50 (s, 1H), 7.46-7.37 (m, 2H), 6.87 (d, J=8.4 Hz, 1H), 6.33 (s, 2H), 4.55 (dd, J=3.1, 9.9 Hz, 1H), 4.50-4.45 (m, 1H), 4.43-4.34 (m, 1H), 2.12 (s, 6H); LCMS: [M+H]+=435.38.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (36.3 mg, 0.103 mmol) and (R)—N,N-dimethyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)chroman-4-amine hydrochloride (35 mg, 0.103 mmol) to give the title compound (15.3 mg, 32% yield). 1H NMR (500 MHz, DMSO-d6) δ=11.15 (br s, 1H), 8.26 (dd, J=1.7, 8.3 Hz, 1H), 7.81 (s, 1H), 7.72 (dd, J=1.2, 8.3 Hz, 1H), 7.63 (d, J=10.1 Hz, 1H), 7.55 (s, 1H), 7.42 (br d, J=5.4 Hz, 1H), 7.31 (br d, J=8.3 Hz, 1H), 6.78 (d, J=8.4 Hz, 1H), 6.32 (s, 2H), 4.37-4.28 (m, 1H), 4.17-4.06 (m, 1H), 3.80 (dd, J=5.6, 8.3 Hz, 1H), 2.20 (s, 6H), 2.03-1.86 (m, 2H); LCMS: [M+H]+=449.30.
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (40 mg, 0.119 mmol) and 2-methoxy-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzonitrile (37 mg, 0.143 mmol, prepared from 5-bromo-2-methoxybenzonitrile using similar procedures to those described earlier) to give the title compound (39 mg, 84% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ 7.94 (d, J=2.20 Hz, 2H), 7.91 (d, J=7.95 Hz, 1H), 7.86 (d, J=8.80 Hz, 1H), 7.71 (d, J=10.27 Hz, 1H), 7.49 (dd, J=1.47, 7.95 Hz, 1H), 7.46 (s, 1H), 7.30 (d, J=9.05 Hz, 1H), 6.38 (s, 2H), 3.94 (s, 3H), 3.40 (dt, J=2.63, 6.51 Hz, 2H), 2.95 (t, J=6.54 Hz, 2H); LCMS: [M+H]+=389.31.
Step 1: N-(5-bromo-2-methoxybenzyl)-N-methylethanamine
Prepared using 5-bromo-2-methoxybenzaldehyde (335 mg, 1.558 mmol) and N-ethylmethylamine (0.535 ml, 6.23 mmol) to give the product as a colorless oil (347 mg, 82% yield). LCMS: [M+H]+=258.27.
Step 2: N-(2-methoxy-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)-N-methylethanamine
Prepared from N-(5-bromo-2-methoxybenzyl)-N-methylethanamine (240 mg, 0.837 mmol) to give the product as a black solid which was taken to the next step without any purification. (255 mg, 90% yield); LCMS: [M+H]+=306.36.
Step 3: 6-(2-amino-5-(3-((ethyl(methyl)amino)methyl)-4-methoxyphenyl)-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-fluoroisoquinolin-1(2H)-one (51.9 mg, 0.147 mmol) and N-(2-methoxy-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)-N-methylethanamine (60 mg, 0.177 mmol) to afford the title compound as a beige solid (28 mg, 40% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 11.17 (br. s., 1H), 8.28 (d, J=8.07 Hz, 1H), 7.83 (s, 1H), 7.74 (d, J=8.31 Hz, 1H), 7.67 (d, J=10.03 Hz, 1H), 7.50 (br. s., 1H), 7.43 (br. s., 2H), 7.03 (d, J=8.44 Hz, 1H), 6.34 (br. s., 2H), 3.81 (s, 3H), 3.48 (br. s., 2H), 2.37-2.45 (m, 2H), 2.07-2.21 (m, 3H), 1.03 (br. s., 3H); LCMS: [M+H]+=451.55
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-4-methylisoquinolin-1(2H)-one (51.3 mg, 0.147 mmol) and N-(2-methoxy-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)-N-methylethanamine (60 mg, 0.177 mmol) to afford the title compound as a beige solid (28 mg, 40% yield). 1H NMR (500 MHz, DMSO-d6) ppm 11.10 (br. s., 1H), 8.29 (d, J=8.19 Hz, 1H), 7.73 (s, 1H), 7.67 (d, J=9.90 Hz, 1H), 7.63 (d, J=8.07 Hz, 1H), 7.36-7.56 (m, 1H), 7.36-7.56 (m, 2H), 7.05 (br. s., 2H), 6.29 (br. s., 2H), 3.81 (br. s., 3H), 3.48 (br. s., 2H), 2.36-2.44 (m, 2H), 2.25 (s, 3H), 2.03-2.23 (m, 3H), 0.94-1.10 (m, 3H); LCMS: [M+H]+=447.35
To a degassed suspension of 8-bromo-6-fluoro-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one (43 mg, 0.165 mmol), 5-(4-(4-(cyclopropylmethyl)piperazin-1-yl)phenyl)-6-fluoro-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (90 mg, 0.198 mmol), Potassium phosphate tribasic (105 mg, 0.496 mmol) and XPhos Pd G2 (15.61 mg, 0.020 mmol) was added a 5:1 mixture of Acetonitrile (4 ml): Water (0.800 ml). The system was flushed with nitrogen then heated at 90° C. overnight on the stir plate. The reaction was concentrated onto celite and purified by silica gel chromatography to give the title compound (3.2 mg, 3.83% yield) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.47 (t, J=5.87 Hz, 1H), 7.60 (d, J=10.15 Hz, 1 H), 7.40 (d, J=8.07 Hz, 2H), 7.20 (d, J=10.64 Hz, 1H), 7.04 (s, 1H), 6.97 (d, J=8.80 Hz, 2 H), 6.29 (s, 2H), 4.22 (t, J=5.32 Hz, 2H), 3.16-3.20 (m, 4H), 2.56-2.60 (m, 4H), 2.22 (d, J=6.60 Hz, 2H), 0.86 (d, J=3.42 Hz, 1H), 0.45-0.50 (m, 2H), 0.10 (q, J=4.65 Hz, 2H); LCMS: [M+H]+=506.66.
Prepared from 8-bromo-6-fluoro-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one (39.5 mg, 0.152 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (80 mg, 0.182 mmol) to give 8-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-6-fluoro-3,4-dihydrobenzo[f][1,4]oxazepin-5(2H)-one (15.6 mg, 20.81% yield) as beige solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.47 (t, J=5.93 Hz, 1H), 7.60 (d, J=10.15 Hz, 1H), 7.40 (d, J=8.19 Hz, 2H), 7.19 (d, J=10.64 Hz, 1H), 7.04 (s, 1H), 6.96 (d, J=8.68 Hz, 2H), 6.29 (s, 2 H), 4.22 (t, J=5.32 Hz, 2H), 3.28-3.31 (m, 2H), 3.15 (br. s., 4H), 2.68 (br. s., 1H), 2.59 (br. s., 4H), 1.01 (d, J=6.24 Hz, 6H); LCMS: [M+H]+=494.63.
Prepared from 5-bromo-7-fluoroisoindolin-1-one (35 mg, 0.152 mmol) and 6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-amine (80 mg, 0.183 mmol) to give 5-(2-amino-6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-3-yl)-7-fluoroisoindolin-1-one (1.5 mg, 2.1% yield) as an off-white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.57 (br. s., 1H), 7.63 (d, J=10.03 Hz, 1H), 7.50 (s, 1H), 7.42 (d, J=7.70 Hz, 2H), 7.34 (d, J=10.39 Hz, 1H), 6.99 (d, J=7.70 Hz, 2H), 6.28 (br. s., 2H), 4.41 (br. s., 2H), 3.11-3.23 (m, 4H), 2.81-2.90 (m, 1H), 2.59-2.79 (m, 4H), 1.08 (br. s., 6H); LCMS: [M+H]+=464.55.
Step 1: ethyl 2-(3-(3-nitrophenyl)oxetan-3-yl)acetate
To a stirred solution of [Rh(COD)Cl]2 (1.4 g, 0.04 mmol) in 1,4-Dioxane (100 mL) was added 1.5M KOH solution (47 mL, 70.4 mmol) stirred for 15 mins and then were added 3-nitrophenylboronic acid (10 g, 70.4 mmol) and ethyl 2-(oxetan-3-ylidene)acetate (23.5 g, 140.9 mmol) at RT for 16 h. The reaction mixture was quenched with ice water (200 mL) and extracted with ethyl acetate (2×200 mL), the combined organic layers were dried over Na2SO4 and concentrated under reduced pressure gave the crude product which was purified by sgc (100-200 mesh) eluting with 15-20% ethyl acetate in pet ether to afford the product (6 g, 32%) as a pale brown sticky solid. LCMS: [M+H]+=266.12
Step 2: 2-(3-(3-nitrophenyl)oxetan-3-yl)acetohydrazide
To a stirred solution of ethyl 2-(3-(3-nitrophenyl)oxetan-3-yl)acetate (6 g, 22.6 mmol) in ethanol (60 mL) was added hydrazine hydrate (7.1 mL, 226.4 mmol) at RT. The reaction mixture was stirred at 85° C. for 16 h before cooling to RT. The reaction mixture was concentrated under reduced pressure to give crude compound. Which was washed with 10% ethyl acetate in pet ether (50 mL) to afford the product (6 g, 92%) as a brown sticky solid. TLC system: 10% MeOH in DCM; Rf=0.7.
Step 3: N-methyl-2-(2-(3-(3-nitrophenyl)oxetan-3-yl)acetyl)hydrazine-1-carbothioamide
To a stirred solution of 2-(3-(3-nitrophenyl)oxetan-3-yl)acetohydrazide (6 g, 23.9 mmol) in THF (120 mL) was added isothiocyanatomethane (3.5 g. 47.8 mmol) and TEA (3.2 mL, 23.9 mmol) at 0° C. The reaction mixture was stirred at RT for 16 h. The precipitate was filtered and washed with THF (30 mL) and dried under vacuum to afford the product (4 g, 52%) as a pale brown solid. LCMS: [M+H]+=325.17.
Step 4: 4-methyl-5-((3-(3-nitrophenyl)oxetan-3-yl)methyl)-4H-1,2,4-triazole-3-thiol
To a stirred solution of N-methyl-2-(2-(3-(3-nitrophenyl)oxetan-3-yl)acetyl)hydrazine-1-carbothioamide (4 g, 12.3 mmol) in 1M Aq. NaOH solution (185 mL, 185.2 mmol) at RT stirred for 16 h. The reaction mixture was acidified with 1N HCl pH up to 5.0 at 15 to 20° C. and stirred for 30 min. Solid compound was filtered and washed with water (30 mL) and dried under vacuum to afford the product (3 g, 80%) as a pale brown solid. LCMS: [M+H]+=307.11
Step 5: 4-methyl-3-((3-(3-nitrophenyl)oxetan-3-yl)methyl)-4H-1,2,4-triazole
To a solution of 4-methyl-5-((3-(3-nitrophenyl)oxetan-3-yl)methyl)-4H-1,2,4-triazole-3-thiol (3 g, 9.8 mmol) in water (30 mL) was added NaNO2 (6.8 g, 98.0 mmol) at RT followed by addition of HNO3 (4.1 mL, 98.0 mmol) dropwise using an addition funnel for 15 min at 0° C. and stirred for 1 h at same temperature. The reaction mass was basified with saturated NaHCO3 solution pH up to 8.0. The reaction mass was extracted with EtOAc (2×200 mL), the combined organic layers was dried over Na2SO4 and concentrated to afford the product (2.4 g, 89%) as a pale brown solid. LCMS: [M+H]+=275.09.
Step 6: 3-(3-((4-methyl-4H-1,2,4-triazol-3-yl)methyl)oxetan-3-yl)aniline
To a solution of 4-methyl-3-((3-(3-nitrophenyl)oxetan-3-yl)methyl)-4H-1,2,4-triazole (2.4 g, 8.8 mmol) in ethanol (48 mL) were added Fe (2.45 g, 43.8 mmol) and NH4Cl (2.34 g, 43.8 mmol) at RT. The reaction mass was stirred at 80° C. for 2 h before cooling to RT. The reaction mixture was filtered through celite pad and washed with ethanol (2×100 mL). The filtrate was concentrated under reduced pressure to give crude compound. Which was purified by column chromatography (silica gel, 100-200 mesh), using 6 to 10% MeOH in DCM as an eluent to afford the product (1.8 g, 84% yield) as a light brown solid. LCMS: [M +H]+=245.34.
Step 7: 3-((3-(3-bromophenyl)oxetan-3-yl)methyl)-4-methyl-4H-1,2,4-triazole
To a solution of 3-(3-((4-methyl-4H-1,2,4-triazol-3-yl)methyl)oxetan-3-yl)aniline (4.2 g, 15.3 mmol) in ACN (42 mL) were added CuBr2 (3.42 g, 15.3 mmol) and t-BuNO2 (4.55 mL, 38.3 mmol) at 0° C. The reaction mass was stirred at 70° C. for 5 h before cooling to RT. The reaction mixture was quenched with ice cold water (200 mL). Then, the reaction mixture was filtered through celite pad and washed with ethyl acetate (2×100 mL). The filtrate was extracted with ethyl acetate (2×100 mL). The combined organic layer was dried with sodium sulphate and concentrated under reduced pressure to give crude compound. Which was purified by column chromatography (silica gel, 100-200 mesh), by using 5 to 7% MeOH in DCM as an eluent to afford the crude product (1.6 g, 70% pure by LCMS) as a light green sticky solid. It was again purified by reverse phase column by using 0 to 30% 0.1% TFA in water: ACN as an eluent to afford the purified product (1.3 g, 28%) as an off white solid.; LCMS: [M+H]+=308.12.
Step 8: 6-(2-amino-6-fluoro-5-(3-(3-((4-methyl-4H-1,2,4-triazol-3-yl)methyl)oxetan-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one I-638
Prepared from 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (22.38 mg, 0.058 mmol) and 3-((3-(3-bromophenyl)oxetan-3-yl)methyl)-4-methyl-4H-1,2,4-triazole (15 mg, 0.049 mmol) to give 6-(2-amino-6-fluoro-5-(3-(3-((4-methyl-4H-1,2,4-triazol-3-yl)methyl)oxetan-3-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (15.3 mg, 0.032 mmol, 64.9% yield) as a white solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 8.19 (s, 1H), 7.94 (br. s., 1H), 7.92 (d, J=7.95 Hz, 1H), 7.52 (d, J=10.03 Hz, 1H), 7.45 (d, J=7.95 Hz, 1H), 7.39-7.44 (m, 2H), 7.32 (t, J=7.70 Hz, 1H), 7.01 (s, 1H), 6.86 (d, J=7.70 Hz, 1H), 6.32 (s, 2H), 4.93 (d, J=6.11 Hz, 2H), 4.88 (d, J=6.11 Hz, 2H), 3.49 (s, 2H), 3.41 (td, J=6.48, 2.57 Hz, 2H), 2.97 (t, J=6.48 Hz, 2H), 2.84 (s, 3H); LCMS [M+H]+ 485.26.
Step 1: 6-((5-bromo-6-fluoropyridin-2-yl)amino)-3,4-dihydroisoquinolin-1(2H)-one
To a vial containing 6-bromo-3,4-dihydro-2h-isoquinolin-1-one (100 mg, 0.442 mmol), 6-amino-3-bromo-2-fluoropyridine (93 mg, 0.487 mmol), tris(dibenzylideneacetone)dipalladium (0) (20.25 mg, 0.022 mmol), Xantphos (25.6 mg, 0.044 mmol), and potassium phosphate tribasic (235 mg, 1.106 mmol) was added 1,4-dioxane (6 ml). The system was flushed with nitrogen and heated at 100° C. overnight. The reaction was concentrated onto celite and purified by flash chromatography (silica gel) eluting with 0-100% EtOAc/Hexanes. The desired fractions were collected, concentrated and dried under vacuum to afford 6-((5-bromo-6-fluoropyridin-2-yl)amino)-3,4-dihydroisoquinolin-1(2H)-one (131 mg, 0.390 mmol, 88% yield) as a yellow solid. LCMS [M+H]+ 336.08.
Step 2: 6-((6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-yl)amino)-3,4-dihydroisoquinolin-1(2H)-one, hydrochloride
The free base of the product was prepared from 6-((5-bromo-6-fluoropyridin-2-yl)amino)-3,4-dihydroisoquinolin-1(2H)-one (45 mg, 0.134 mmol) and 4-(4-isopropylpiperazinyl)phenylboronic acid, pinacol ester (53.1 mg, 0.161 mmol) to give 6-((6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-yl)amino)-3,4-dihydroisoquinolin-1(2H)-one (18.5 mg, 30.1% yield) as a white solid. To a suspension of 6-((6-fluoro-5-(4-(4-isopropylpiperazin-1-yl)phenyl)pyridin-2-yl)amino)-3,4-dihydroisoquinolin-1(2H)-one (18.5 mg, 0.040 mmol) in THF (1 ml) was added hydrochloric acid, 4.0 M in dioxane (0.067 ml, 0.268 mmol). The reaction turned to a solution immediately and was stirred at room temperature for 1 hour. The reaction was concentrated in vacuo and dried on the lyophilizer to afford product as the hydrochloride salt (15.7 mg, 23.6% yield) as a pale-yellow solid. 1H NMR (500 MHz, DMSO-d6) δ ppm 10.84 (br. s., 1H), 9.83 (br. s., 1H), 7.86-7.94 (m, 1H), 7.77 (d, J=8.80 Hz, 1H), 7.67 (br. s., 1H), 7.56-7.63 (m, 2H), 7.47 (d, J=7.95 Hz, 2H), 7.09 (d, J=8.44 Hz, 2H), 6.94 (d, J=8.07 Hz, 1H), 3.90 (d, J=12.35 Hz, 2H), 3.50 (m, J=11.00 Hz, 3H), 3.36 (br. s., 2H), 3.20-3.29 (m, 2H), 3.10-3.19 (m, 2H), 2.87 (t, J=6.05 Hz, 2H), 1.33 (d, J=6.48 Hz, 6H); LCMS [M+H]+=460.06.
Step 1: tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate
Prepared from 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (700 mg, 2.082 mmol) and tert-butyl 4-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]tetrahydro-1(2H)-pyrazinecarboxylate (1011 mg, 2.60 mmol) to afford the product as a beige solid (676 mg, yield 61%) LCMS: [M+H]+=518.47
Step 2: 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, Trifluoroacetic Acid salt
To a solution of the tert-butyl 4-(4-(6-amino-2-fluoro-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperazine-1-carboxylate (676 mg, 1.306 mmoln DCM (2 ml), was added TFA (9.00 ml, 118 mmol) at RT and the reaction mixture was continuously stirred at RT for 1.5 h The reaction mixture was concentrated, and triturated from Methanol to yield the title compound as a beige solid (636 mg, 92% yield). LCMS: [M+H]+=418.26.
Step 3: Preparation of 6-(2-amino-5-(4-(4-((2,2-difluorocyclopropyl)methyl)piperazin-1-yl)phenyl)-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a slurry of the product of the foregoing procedure (75 mg, 0.141 mmol) in DMF (1.5 mL) at RT, was added N,N-diisopropylethylamine (0.246 ml, 1.411 mmol). After 5 min of stirring, 1-bromomethyl-2,2-difluorocyclopropane (72.4 mg, 0.423 mmol) was added and the reaction mixture was stirred at 60° C. for 5 h. The reaction mixture was partitioned between water (6 mL) and DCM (3 mL), the organic phase was separated, aqueous phase was extracted with DCM (1×3 ml), the combined organic phase was dried over Na2SO4 and concentrated. It was purified by silica gel chromatography, eluting with dichloromethane containing 0-4% Methanol and 0-0.4% NH4OH to afford the title compound as a beige solid (49 mg, 65%). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.83-8.01 (m, 2H), 7.58 (d, J=10.15 Hz, 1H), 7.46 (d, J=8.07 Hz, 1H), 7.44 (s, 1H), 7.40 (d, J=8.07 Hz, 2H), 6.98 (d, J=8.68 Hz, 2H), 6.09-6.26 (m, 2H), 3.52-3.62 (m, 2H), 3.12-3.19 (m, 4H), 3.18 (br. s., 4H), 2.95 (t, J=6.36 Hz, 2H), 2.53-2.69 (m, 5H), 2.53-2.69 (m, 5H), 2.36 (dd, J=12.78, 8.13 Hz, 1H), 1.79-1.95 (m, 1H), 1.60 (tdd, J=11.97, 11.97, 7.67, 4.34 Hz, 1H), 1.16-1.26 (m, 1H); LCMS: [M+H]+=508.45
Prepared as per the procedure analogous to that of Example 86, step 3, by heating the intermediate of 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one, trifluoroacetic acid salt, (75 mg, 0.141 mmol) from Example 86, step 2 and N,N-diisopropylethylamine (0.246 ml, 1.411 mmol) to afford the title compound as a white solid (43 mg, 57% yield). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.83-8.00 (m, 2H), 7.59 (d, J=10.15 Hz, 1H), 7.44-7.50 (m, 2H), 7.41 (d, J=8.19 Hz, 2H), 6.98 (d, J=8.56 Hz, 2H), 5.98-6.27 (m, 3H), 5.98-6.27 (m, 3H), 5.98-6.27 (m, 3H), 3.41 (d, J=4.03 Hz, 2H), 3.17 (br. s., 4H), 2.96 (t, J=6.30 Hz, 2H), 2.52-2.59 (m, 4H), 2.37 (d, J=6.48 Hz, 2 H), 1.79-1.94 (m, 2H), 1.59 (quin, J=7.31 Hz, 2H); LCMS: [M+H]+=510.39 Example 589: (S)-6-(2-amino-6-fluoro-5-(3-(1-methylpyrrolidin-2-yl)-4-(tetrahydro-2H-pyran-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (I-642)
Step 1: (S)-2-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)-1-methylpyrrolidine
tert-Butyl (S)-2-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)pyrrolidine-1-carboxylate (100 mg, 0.244 mmol) (prepared as per patent WO 2020/193511, PCT/EP2020/058081) was deprotected as described in earlier examples to isolate (S)-2-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)pyrrolidine (83 mg, 99% yield) as a beige solid which was taken up in acetonitrile (3 mL) and Iodomethane (0.02 ml, 0.253 mmol) was added at 0-5° C. The reaction mixture was stirred overnight at RT, concentrated, and partitioned between water (4 ml) and dichloromethane (3 mL). The organic phase was separated, the aqueous phase was extracted with dichloromethane (3 mL), the combined org phase was washed with brine, dried over Na2SO4 and concentrated to yield the title compound as a beige solid (66 mg, 72% yield, 85% purity). LCMS: [M+H]+=324.29.
Step 2: (S)-6-(2-amino-6-fluoro-5-(3-(1-methylpyrrolidin-2-yl)-4-(tetrahydro-2H-pyran-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
Prepared from (S)-2-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)-1-methylpyrrolidine 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one and 6-(2-amino-5-bromo-6-fluoropyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one to yield the title compound as a beige solid (54 mg, 59% yield with >95% purity). 1H NMR (500 MHz, Methanol-d4) δ 7.98-7.91 (m, 1H), 7.70-7.64 (m, 1H), 7.62 (s, 1H), 7.59-7.54 (m, 1H), 7.48-7.43 (m, 1H), 7.43-7.40 (m, 1H), 7.39 (s, 1H), 4.85-4.78 (m, 1H), 4.03-3.92 (m, 2H), 3.82-3.70 (m, 1H), 3.62-3.53 (m, 2H), 3.49-3.42 (m, 2H), 3.34-3.25 (m, 1H), 3.13-3.04 (m, 1H), 3.00-2.92 (m, 2H), 2.76-2.63 (m, 3H), 2.54-2.45 (m, 1H), 2.31-2.07 (m, 3H), 1.98-1.86 (m, 1H), 1.78-1.67 (m, 1H), 1.66-1.60 (m, 1H), 1.58-1.49 (m, 1H); LCMS: [M+H]+=501.36.
Prepared from 7-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-2-methylquinazolin-4(3H)-one and tert-butyl (S)-2-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)pyrrolidine-1-carboxylate (prepared as per patent WO 2020/193511, PCT/EP2020/058081) (52 mg, 0.126 mmol) followed by deprotection to give the title compound as an off white solid (21 mg, 69% yield with >95% purity). 1H NMR (500 MHz, Methanol-d4) δ 8.21-8.13 (m, 1H), 7.69-7.66 (m, 1H), 7.66-7.62 (m, 1H), 7.56-7.51 (m, 2H), 7.39-7.35 (m, 1H), 7.30-7.26 (m, 1H), 4.47-4.40 (m, 1H), 3.99-3.93 (m, 2H), 3.56-3.49 (m, 2H), 3.18-3.13 (m, 1H), 3.13-3.05 (m, 1H), 2.99-2.90 (m, 1H), 2.39-2.34 (m, 3H), 2.23-2.14 (m, 1H), 1.98-1.84 (m, 2H), 1.82-1.75 (m, 2H), 1.69-1.61 (m, 2H), 1.60-1.55 (m, 1H). LCMS: [M+H]+=500.30.
Prepared from 6-(2-amino-6-fluoro-5-(4-(piperazin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one TFA salt (75 mg, 0.141 mmol) and 2-(2-bromoethyl)-1,1-difluorocyclopropane (78 mg, 0.423 mmol) to afford the title compound as a beige solid (44 mg, 59% yield with >95% purity). 1H NMR (500 MHz, DMSO-d6) δ ppm 7.85-8.02 (m, 2 H), 7.55-7.65 (m, 1H), 7.34-7.52 (m, 4H), 6.98 (d, J=8.68 Hz, 2H), 6.17 (s, 2H), 3.42-3.46 (m, 2H), 3.13-3.19 (m, 4H), 2.96 (t, J=6.30 Hz, 2H), 2.55 (br. s., 4H), 2.42 (t, J=6.91 Hz, 2H), 1.63-1.76 (m, 2H), 1.47-1.62 (m, 2H), 1.13 (ddt, J=9.92, 6.74, 3.53, 3.53 Hz, 1 H); LCMS: [M+H]+=522.49.
Prepared from 1-(5-bromo-2-(tetrahydro-2H-pyran-4-yl)phenyl)-N,N-dimethylmethanamine (60 mg, 0.157 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (78 mg, 0.204 mmol) to yield the title compound as an off white solid (28 mg, 37% yield). 1H NMR (500 MHz, METHANOL-d4) δ ppm 8.06 (d, J=7.95 Hz, 1H), 7.69 (d, J=9.78 Hz, 1H), 7.53 (dd, J=8.01, 1.53 Hz, 1H), 7.44-7.50 (m, 3H), 7.39 (d, J=8.19 Hz, 1H), 4.04-4.13 (m, 2H), 3.51-3.66 (m, 7H), 3.08 (t, J=6.60 Hz, 2H), 2.29 (s, 6H), 1.83-1.93 (m, 2H), 1.73 (d, J=12.47 Hz, 2 H)LCMS: [M+H]+=475.35.
Prepared from (2S)-2-(3-bromophenyl)pyrrolidine (39 mg, 0.172 mmol) and 6-(2-amino-6-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (83 mg, 0.216 mmol) to give the title compound as a beige solid. (55 mg, 75% yield). 1H NMR (500 MHz, Methanol-d4) δ ppm 8.09-8.03 (m, 1H), 7.73-7.67 (m, 1H), 7.57-7.51 (m, 2H), 7.49-7.43 (m, 2H), 7.42-7.37 (m, 1H), 7.36-7.32 (m, 1H), 4.13-4.07 (m, 1H), 3.60-3.55 (m, 2H), 3.24-3.17 (m, 1H), 3.11-3.05 (m, 2H), 3.01-2.93 (m, 1H), 2.32-2.22 (m, 1H), 2.05-1.97 (m, 1H), 1.96-1.87 (m, 1H), 1.82-1.72 (m, 1H); LCMS: [M+H]+=403.40.
Step 1: 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a degassed suspension of 6-bromo-3,4-dihydroisoquinolin-1(2H)-one (3 g, 13.3 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (5.07 g, 20 mmol), KOAc (3.92 g, 40 mmol) in 1,4-dioxane (30 mL) was added a Pd(dppf)Cl2-DCM (1.08 g, 1.3 mmol). The reaction mixture was heated at 85° C. for 16 h under argon atmosphere before cooling to RT. The reaction mixture was filtered through a Celite® bed, which was washed in with ethyl acetate (100 mL). The combined filtrate was concentrated under reduced pressure and the residue obtained was purified by column chromatography (silica gel 100-200 mesh), eluting with 50-70% EtOAc in pet ether to afford the product (3 g, 82%) as a pale yellow solid.
Step 2. 6-(2-amino-5-bromopyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a degassed suspension of 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (2.5 g, 9.1 mmol) 5-bromo-3-iodopyridin-2-amine (2.72 g, 9.1 mmol) and K2CO3 (2.52 g, 18.3 mmol) in DMF: H2O (40:5 mL) was added Pd(PPh3)2Cl2 (0.64 g, 0.9 mmol) at RT−65° C. over 16 h before cooling to RT. The reaction mixture was poured into ice water (100 mL) extracted with CH2Cl2 (2×100 mL) washed with water (50 mL), followed by brine (50 mL). The separated organic layer was dried over Na2SO4 and concentrated under reduced pressure and the residue obtained was purified by column chromatography (silica gel 100-200 mesh), eluting with 0-10% MeOH in DCM to afford the product (1.3 g, 44%) as a pale yellow solid. LCMS: [M+H]+=318.18.
Step 3: tert-butyl 4-(4-(6-amino-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate
A mixture of 6-(2-amino-5-bromopyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (125 mg, 0.393 mmol), Cs2CO3 (384 mg, 1.18 mmol), 4-(N-Boc-piperidino)phenylboronic acid pinacol ester (183 mg, 0.471 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (34.5 mg, 0.047 mmol) in DME (4.0 mL) and H2O (0.800 mL) in a microwave vial was flushed with argon. The mixture was heated in a microwave reactor at 80° C. for 2 h. The reaction mixture was partitioned between EtOAc and brine, the organic phase was separated and the aqueous phase was extracted with CH2Cl2. The combined organic layers were dried over Na2SO4 and concentrated onto Celite®. Purification by silica gel chromatography (4 g), eluting with CH2Cl2 containing 0-2% MeOH and 0-0.2% NH4OH afforded the product as a beige solid (137 mg, 70%). LCMS: [M +H]+=499.71
Step 4: 6-(2-amino-5-(4-(piperidin-4-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a slurry of tert-butyl 4-(4-(6-amino-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)phenyl)piperidine-1-carboxylate (137 mg, 0.275 mmol) in CH2Cl2 (3 mL) was added HCl in dioxane (3.43 mL of a 4.0 N solution, 13.74 mmol) and the mixture was stirred at RT for 1 h. The mixture was concentrated to dryness, dissolved in MeOH, passed through a porapak cartridge to collect, after removing the solvent, the free base as an off-white solid (101 mg, 88%)1H NMR (500 MHz, DMSO-d6) δ 8.34-8.24 (m, 1H), 7.99-7.90 (m, 2H), 7.67-7.61 (m, 1H), 7.60-7.54 (m, 2H), 7.53-7.46 (m, 2H), 7.31-7.24 (m, 2H), 5.83 (s, 2H), 3.45-3.40 (m, 2H), 3.08-3.00 (m, 2H), 2.99-2.94 (m, 2H), 2.65-2.56 (m, 3H), 1.74-1.66 (m, 2H), 1.58-1.48 (m, 2H). LCMS: [M+H]+=399.63.
Step 1: 5-bromo-3-chloropyridin-2-amine
To a solution of 3-chloropyridin-2-amine (4.0 g, 31.1 mmol) in CH3CN (100 mL) was added N-bromosuccinimide (5.54 g, 31.1 mmol) in several portions at 0° C. The reaction was allowed to warm slowly and stirred at RT overnight. The reaction mixture was stored at −20° C. for 5 d. Light orange crystals (plates) that formed were collected and rinsed with small amount of MeCN to afford the product (5.6 g, 87%) as a light orange solid. 1H NMR (500 MHz, CDCl3) δ 8.40-8.74 (m, 0.5H), 8.03 (d, J=2.1 Hz, 1H), 7.63 (d, J=2.1 Hz, 1H), 4.98 (br s, 2H), 2.78 (s, 2H).
Step 2: 3-chloro-5-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)pyridin-2-amine
A vial charged with 5-bromo-3-chloropyridin-2-amine (100 mg, 0.482 mmol), Cs2CO3 (471 mg, 1.45 mmol), 1-(tetrahydro-pyran-4-yl)-1H-pyrazole-4-boronic acid pinacol ester (147 mg, 0.530 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (35.4 mg, 0.048 mmol) was suspended in water (2 mL). The reaction was degassed, and DME (4 mL) was added. The reaction was degassed again by evacuation-refill with argon then heated sealed under microwave irradiation at 100° C. for 3 h. The reaction mixture was concentrated under reduced pressure, deposited on Celite® and purified by flash silica gel chromatography, eluting with 8% MeOH in CH2Cl2, to afford the product as a tan solid (95 mg, 71%). LCMS: [M+H]+=278.99.
Step 3: 6-(2-amino-5-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a mixture of 6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,4-dihydroisoquinolin-1(2H)-one (37.6 mg, 0.138 mmol), 3-chloro-5-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)pyridin-2-amine (32 mg, 0.115 mmol), K3PO4 (97 mg, 0.459 mmol), palladium(II) acetate (2.58 mg, 0.011 mmol) and XPhos (13.68 mg, 0.029 mmol) under an atmosphere of argon before water (2 mL) and CH3CN (3 mL) were added. The mixture was degassed with argon and heated in a microwave reactor at 100° C. for 2 h. The reaction mixture was concentrated under reduced pressure, deposited on Celite® and purified by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in (H2O+0.05% TFA)) eluting at 30% MeOH, pooled fractions: 17-25 to afford the TFA salt of the title compound as a white solid (19.4 mg, 33%). LCMS: [M+H]+=390.43. 1H NMR (500 MHz, MeOD-d4) δ 8.21 (s, 1H), 8.17 (br d, J=9.7 Hz, 2H), 8.11 (d, J=8.0 Hz, 1H), 7.93 (s, 1H), 7.54 (br d, J=8.0 Hz, 1H), 7.50 (s, 1H), 4.39-4.50 (m, 1H), 4.04-4.10 (m, 2H), 3.52-3.63 (m, 4H), 3.05-3.12 (m, 2H), 2.03-2.14 (m, 4H).
Step 1: 3-chloro-5-(4-(pyrrolidin-1-yl)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloropyridin-2-amine (0.047 g, 0.228 mmol), 1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyrrolidine (0.052 g, 0.190 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.014 g, 0.019 mmol) and Cs2CO3 (0.155 g, 0.476 mmol). After the vial was sealed with a cap and septum the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (2 mL) and water (1 mL) were added via syringe and the vessel was evacuated and backfilled with nitrogen an additional time. The reaction was heated to 80° C. for 16 h, concentrated onto Celite® and purified by flash silica gel chromatography (0.5-10% MeOH/DCM+0.5% NH4OH) to afford the product (45 mg, 86% yield). LCMS: [M+H]+=317.3.
Step 2: 6-(2-amino-5-(4-(pyrrolidin-1-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
A 30 mL vial with magnetic stir bar was charged 3-chloro-5-(4-(pyrrolidin-1-yl)phenyl)pyridin-2-amine (0.0225 g, 0.082 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.027 g, 0.099 mmol) and XPhos Pd G2 (6.47 mg, 8.22 μmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. Butan-1-ol (1 mL) and aqueous K3PO4 (0.158 mL, 0.205 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 90° C. for 18 h in an aluminum block, allowed to cool to RT and concentrated onto Celite. Purification by flash chromatography (eluting with 0.5-10% MeOH/DCM+0.5% NH4OH) followed by reverse phase flash chromatography (eluting with 5-60% MeCN/water+0.1% formic acid) afforded the title compound (15 mg, 48%) as a yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 8.22 (d, J=2.3 Hz, 1H), 7.9-8.0 (m, 2H), 7.55 (d, J=2.3 Hz, 1H), 7.4-7.5 (m, 4H), 6.58 (br d, J=8.7 Hz, 2H), 5.65 (s, 2H), 3.4-3.4 (m, 2H), 3.24 (br t, J=6.4 Hz, 4H), 2.96 (br t, J=6.4 Hz, 2H), 1.9-2.0 (m, 4H). LCMS: [M+H]+=385.26.
Step 1: 1-(4-chlorophenyl)piperidine
A 30 mL vial was charged with 1-chloro-4-iodobenzene (0.250 g, 1.048 mmol), tris(dibenzylideneacetone)dipalladium (0) (0.029 g, 0.031 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.055 g, 0.094 mmol) and potassium tert-butoxide (0.353 g, 3.15 mmol). The vial was sealed with a cap and septum and then the reaction vessel was evacuated and backfilled with nitrogen. Toluene (5 mL) and piperidine (0.104 mL, 1.05 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 100° C. for 18 h, allowed to cool to RT, loaded onto Celite® and purified by flash silica gel chromatography (1-15% EtOAc/hexanes) to afford the product (159 mg, 77%). LCMS: [M+H]+=196.27.
Step 2: 1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperidine
A 30 mL vial was charged with 1-(4-chlorophenyl)piperidine (0.159 g, 0.813 mmol), bis(pinacolato)diboron (0.309 g, 1.22 mmol), KOAc (0.159 g, 1.63 mmol) and XPhos Pd G2 (0.048 g, 0.061 mmol). The vial was evacuated and backfilled with nitrogen. 1,4-Dioxane (5 mL) was added and the vial was evacuated and backfilled an additional time. The reaction mixture was then heated to 110° C. in an aluminum block for 18 h, concentrated onto Celite® and purified by flash (1-10% EtOAc/hexanes) to afford the product (140 mg, 60%). LCMS: [M+H]+=287.93.
Step 3: 3-chloro-5-(4-(piperidin-1-yl)phenyl)pyridin-2-amine
A 30 mL vial was charged with 5-bromo-3-chloropyridin-2-amine (0.121 g, 0.585 mmol), 1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)piperidine (0.14 g, 0.487 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (0.036 g, 0.049 mmol) and Cs2CO3 (0.397 g, 1.22 mmol). After the vial was sealed with a cap and septum the reaction vessel was evacuated and backfilled with nitrogen. 1,4-Dioxane (4 mL) and water (2 mL) were added via syringe, the vessel was evacuated and backfilled with nitrogen an additional time. The reaction was heated to 80° C. overnight, concentrated onto Celite® and purified by flash chromatography (0.5-10% MeOH/DCM+0.5% NH4OH) to afford the product (105 mg, 75%). LCMS: [M+H]+=288.01.
Step 4: 5-(2-amino-5-(4-(piperidin-1-yl)phenyl)pyridin-3-yl)isoindolin-1-one
In a 30 mL vial with magnetic stir bar was charged 3-chloro-5-(4-(piperidin-1-yl)phenyl)pyridin-2-amine (0.050 g, 0.174 mmol), 3,4-dihydro-1(2H)-isoquinolinone-6-boronic acid pinacol ester (0.057 g, 0.208 mmol) and XPhos Pd G2 (0.014 g, 0.017 mmol). The vial was sealed with a cap and septum and the reaction vessel was evacuated and backfilled with nitrogen. Butan-1-ol (2 ml) and aqueous K3PO4 (0.334 mL of a 1.3 M solution, 0.434 mmol) were added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction mixture was heated at 90° C. for 18 h in an aluminum block, concentrated onto Celite® and purified by flash silica gel chromatography (0.5-10% MeOH/DCM+0.5% NH4OH) followed by reverse phase flash chromatography (5-60% MeCN/water+0.1% formic acid) to afford the title compound (24 mg, 35%) as a pale yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 8.25 (d, 1H, J=2.3 Hz), 7.9-8.0 (m, 2H), 7.58 (d, 1H, J=2.3 Hz), 7.5-7.5 (m, 4H), 6.97 (br d, 2H, J=8.8 Hz), 5.73 (s, 2H), 3.4-3.4 (m, 2H), 3.1-3.2 (m, 4H), 2.97 (br t, J=6.3 Hz, 2H), 1.63 (br s, 4H), 1.55 (br d, J=4.4 Hz, 2H). LCMS: [M+H]+=399.18.
Step 1: 6-(2-amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a degassed suspension of 6-(2-amino-5-bromopyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (2.5 g, 7.8 mmol, prepared according to the procedure described in Example 1), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane (3 g, 7.8 mmol), KOAc (2.32 g, 23.6 mmol) in 1,4-dioxane (40 mL) was added a Pd(dppf)Cl2. CH2Cl2 (0.64 g, 0.7 mmol) and then, the reaction mixture was again degassed with argon for 10 min. The reaction mixture was heated to 85° C. for 16 h under argon atmosphere. The reaction mixture was filtered through a Celite® bed, which was washed with EtOAc (50 mL). The combined filtrate was concentrated under reduced pressure and the residue was purified by washed with n-pentane followed by diethyl ether to afford the product (2 g, 69%) as a brown solid. TLC system: 5% MeOH: CH2Cl2; Rf=0.3.
Step 2: (3aR,6aS)-5-(4-iodophenyl)hexahydro-1H-furo[3,4-c]pyrrole
A sealed mixture of hexahydro-1H-furo[3,4-c]pyrrole hydrochloride (0.564 g, 3.77 mmol) and K3PO4 (1.60 g, 7.54 mmol) in DMF (20 mL) was stirred at RT for 16 h. The mixture was then degassed with a stream of N2 and then copper (I) iodide (0.144 g, 0.754 mmol), (R)-(+)-1,1′-bi-2-naphthol (0.216 g, 0.754 mmol) and 1,4-diiodobenzene (1.24 g, 3.77 mmol) were added as solids. Then degassing step was repeated. The mixture was then stirred sealed at 40° C. for 12 h. The reaction mixture was diluted with EtOAc and filtered through Celite®. The reaction mixture was concentrated under reduced pressure, and purified by flash silica gel chromatography, eluting with CH2Cl2, to afford the product as a yellow solid (659.0 mg, 50% based on purity of 90%); LCMS: [M+H]+=315.99.
Step 3: 6-(2-amino-5-(4-((3aR,6aS)-tetrahydro-1H-furo[3,4-c]pyrrol-5(3H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
(3aR,6aS)-5-(4-Iodophenyl)hexahydro-1H-furo[3,4-c]pyrrole (67.1 mg, 0.192 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (14.02 mg, 0.019 mmol), 6-(2-amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (70 mg, 0.192 mmol) and Cs2CO3 (187 mg, 0.575 mmol) were suspended in water (2 mL), degassed with N2 and DME (4 mL) was added. Degassing was repeated and the reaction mix was heated under microwave irradiation at 90° C. for 1 h. The reaction was treated with 1 mL of 1 M aq HCl and filtered through a Waters PoraPak™ CX column (0.5 g), rinsing with MeOH and eluting with 2 M NH3 in MeOH and then CH2Cl2. The reaction mixture was concentrated under reduced pressure and purified by flash chromatography (25 g SiO2 Biotage® cartridge, using MeOH in CH2Cl2 eluting at 6% MeOH, to afford the title compound (23 mg 28%) as a light yellow solid. 1H NMR (500 MHz, DMSO-d6) δ 8.23 (d, J=2.5 Hz, 1H), 7.94 (br s, 1H), 7.92 (d, J=7.8 Hz, 1H), 7.56 (d, J=2.3 Hz, 1H), 7.43-7.52 (m, 4H), 6.68 (d, J=8.7 Hz, 2H), 5.68 (s, 2H), 3.85 (br dd, J=8.7, 6.7 Hz, 2H), 3.55 (dd, J=8.8, 3.6 Hz, 2H), 3.34-3.44 (m, 4H), 3.14-3.23 (m, 2H), 2.93-3.04 (m, 4 H); LCMS: [M+H]+=427.47.
Step 1: (3aS,6aS)-5-(4-iodophenyl)hexahydro-2H-furo[2,3-c]pyrrole
A sealed mixture of 1,4-diiodobenzene (2 g, 6.06 mmol), (3aS,6aS)-hexahydro-2H-furo[2,3-c]pyrrole hydrochloride (0.907 g, 6.06 mmol) and K3PO4 (2.57 g, 12.1 mmol) in DMF (20 mL) was stirred at RT overnight. The mixture was then degassed with a stream of N2 and then copper (I) iodide (0.231 g, 1.21 mmol) and a (R)-(+)-1,1′-bi-2-naphthol (0.347 g, 1.21 mmol) were added as solids. Then degassing step was repeated. The mixture was then sealed, stirred at RT for 30 min and then at 40° C. for 4 d. The reaction mixture was concentrated under reduced pressure, and purified by flash silica gel chromatography (eluting with MeOH in CH2Cl2 eluting at 0-1% MeOH, to afford the product as a yellow-grey solid (940 mg, 48%) LCMS: [M+H]+=316.14.
Step 2: 6-(2-amino-5-(4-((3aS,6aS)-hexahydro-5H-furo[2,3-c]pyrrol-5-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
(3aS,6aS)-5-(4-Iodophenyl)hexahydro-2H-furo[2,3-c]pyrrole (62.3 mg, 0.192 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (14.06 mg, 0.019 mmol), 6-(2-amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (70 mg, 0.192 mmol) and Cs2CO3 (187 mg, 0.575 mmol) were suspended in water (2 mL). The mixture was degassed with N2 and DME (4 mL) was added. Degassing was repeated and the mixture was heated under microwave irradiation at 90° C. for 1 h, concentrated under reduced pressure and purified by flash silica gel chromatography (eluting with eluting at 6% MeOH in CH2Cl2. The crude material was repurified by preparative HPLC (30 g Biotage® SNAP KP-C18-HS, MeOH in (H2O no acid)) eluting at 70% MeOH. The collected material was then filtered through a Isolute SCX-2 column (0.5 g), rinsing with MeOH and eluting with 2 M NH3 in MeOH to afford the title compound as a light tan solid (13 mg, 15%). 1H NMR (500 MHz, CDCl3) δ 8.23 (d, J=2.3 Hz, 1H), 8.11 (d, J=8.0 Hz, 1H), 7.50 (d, J=2.3 Hz, 1H), 7.44 (dd, J=8.0, 1.6 Hz, 1H), 7.33-7.39 (m, 2H), 7.31 (s, 1H), 6.62 (d, J=7.6 Hz, 2H), 5.93 (br s, 1H), 4.62 (t, J=5.6 Hz, 1H), 4.51 (s, 2H), 3.87-3.94 (m, 1H), 3.79 (td, J=8.0, 5.1 Hz, 1H), 3.48-3.60 (m, 3H), 3.28-3.39 (m, 2H), 3.19 (dd, J=9.7, 4.8 Hz, 1H), 2.92-3.04 (m, 3H), 2.13 (dq, J=12.4, 7.7 Hz, 1H), 1.68-1.86 (m, 1H); LCMS: [M +H]+=427.39
Step 1: tert-butyl 4-(4-(6-amino-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate
To a solution of tert-butyl 4-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate (452.3 mg, 0.991 mmol) and 6-(2-amino-5-bromopyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (303.8 mg, 0.955 mmol) in 1,4-dioxane (6 ml) was added a solution of Cs2CO3 (933 mg, 2.86 mmol) in water (1 mL) at RT. The reaction mixture was degassed under argon for 15 min before adding [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (105 mg, 0.143 mmol). The reaction mixture was heated at 90° C. for overnight until completion of the starting material. Then the reaction mixture was dry loaded onto Celite® and purified by flash silica gel chromatography (eluting with 0-30% CH2Cl2 in MeOH) to afford the product (461.7 mg, 85%) as a dark beige powder. LCMS: [M+H]+=568.30.
Step 2: 6-(2-amino-5-(4-(piperazin-1-yl)-3-(trifluoromethyl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
To a solution of tert-butyl 4-(4-(6-amino-5-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)pyridin-3-yl)-2-(trifluoromethyl)phenyl)piperazine-1-carboxylate (461.7 mg, 0.813 mmol) in CH2Cl2 (4 mL) was added TFA (2 mL, 26.1 mmol) at RT. The reaction mixture was stirred at RT for 30 min. The solvents were removed and the crude mixture was dissolved in MeOH and passed through a PoraPak Rxn CX (15cc-2g) cartridge in a catch & elute method. The cartridge was washed with MeOH (15 mL), then the solution of product in MeOH was added onto the cartridge. The cartridge was rinsed with MeOH (3×15 mL) and the product was released with a solution of NH3 in MeOH (15 mL® 3N). After removing the solvents under vacuum the product was dry loaded and purified by flash silica gel chromatography (0-30%, MeOH/CH2Cl2) to afford the title compound (218.3 mg, 55%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.33 (d, J=2.4 Hz, 1H), 7.98-7.94 (m, 1H), 7.94-7.89 (m, 2H), 7.84 (d, J=2.1 Hz, 1H), 7.70 (d, J=2.4 Hz, 1H), 7.56-7.49 (m, 2H), 7.48 (s, 1H), 5.95 (s, 2H), 3.41 (dt, J=6.5, 2.6 Hz, 2H), 3.17 (s, 1H), 2.96 (t, J=6.5 Hz, 2H), 2.87-2.79 (m, 8H); LCMS: [M+H]+=468.33.
A procedure similar to Example 7 was followed using 3,4-dihydro-2H-1,5-benzodioxepin-7-ylboronic acid (41.8 mg, 0.215 mmol), 6-(2-amino-5-bromopyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (52.7 mg, 0.166 mmol) and DME (3 mL) Cs2CO3 (162 mg, 0.497 mmol) in water (1 mL) and [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (18.18 mg, 0.025 mmol) afforded after purification by flash silica gel chromatography (eluting with 0-15% CH2Cl2 in MeOH) the title compound (47 mg, 73%) as a beige powder. 1H NMR (500 MHz, DMSO-d6) δ 8.25 (d, J=2.4 Hz, 1H), 7.94 (br s, 1H), 7.91 (d, J=7.8 Hz, 1H), 7.59 (d, J=2.4 Hz, 1H), 7.51 (dd, J=1.6, 7.9 Hz, 1H), 7.48 (s, 1H), 7.25 (d, J=2.3 Hz, 1H), 7.22 (dd, J=2.3, 8.3 Hz, 1H), 6.99 (d, J=8.3 Hz, 1H), 5.83 (s, 2H), 4.14 (td, J=5.4, 8.7 Hz, 4H), 3.41 (dt, J=2.8, 6.5 Hz, 2H), 2.96 (t, J=6.5 Hz, 2H), 2.11 (quin, J=5.4 Hz, 2H), 0.02-0.01 (m, 1H); LCMS: [M+H]+=388.42.
Step 1: 2-(4-iodophenyl)octahydropyrrolo[1,2-a]pyrazine
A sealed mixture of octahydropyrrolo[1,2-a]piperazine (509 mg, 4.03 mmol) and K3PO4 (2566 mg, 12.09 mmol) in DMF (20 mL) was stirred at RT for 15 min. 1,4-diiodobenzene (1.33 g, 4.03 mmol) was added as a solid. The mixture was then degassed with a stream of N2 and copper (I) iodide (154 mg, 0.806 mmol) and (R)-(+)-1,1′-bi-2-naphthol (231 mg, 0.806 mmol) were added. The degassing step was repeated. The mixture was then stirred sealed at 40° C. h for 2 d, then diluted with EtOAc, filtered through Celite® and concentrated under reduced pressure. Purification by flash silica gel chromatography (eluting with 8% MeOH in CH2Cl2 afforded the product as a light pink solid (825 mg, 62%). LCMS: [M +H]+=329.18.
Step 2: 6-(2-amino-5-(4-(hexahydropyrrolo[1,2-a]pyrazin-2(1H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
2-(4-Iodophenyl)octahydropyrrolo[1,2-a]pyrazine (62.9 mg, 0.192 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (14.02 mg, 0.019 mmol), 6-(2-amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (70 mg, 0.192 mmol) and Cs2CO3 (187 mg, 0.575 mmol) were suspended in Water (2 mL). The mixture was degassed (evac-refill with N2). DME (4 mL) was added, degassing was repeated and the reaction mixture was heated under microwave irradiation at 90° C. for 1 h. The reaction mixture was concentrated under reduced pressure, deposited on Celite® and purified by flash chromatography (eluting with 8% MeOH in CH2Cl2 and rinsing with MeOH and eluting with 2 M NH3 in MeOH afforded the title compound (19.0 mg, 22% yield based on purity of 96%). LCMS: [M+H]+=440.56. 1H NMR (500 MHz, DMSO-d6) b 8.25 (d, J=2.5 Hz, 1H), 7.88-7.98 (m, 2H), 7.58 (d, J=2.3 Hz, 1H), 7.31-7.52 (m, 4H), 6.99 (d, J=8.8 Hz, 2H), 5.72 (s, 2H), 3.80 (br d, J=9.8 Hz, 1H), 3.65 (br d, J=11.7 Hz, 1H), 3.39-3.44 (m, 2H), 2.93-3.08 (m, 4H), 2.74 (td, J=11.6, 3.2 Hz, 1H), 2.33-2.47 (m, 1H), 2.23 (td, J=11.1, 3.1 Hz, 1H), 1.92-2.10 (m, 2H), 1.64-1.87 (m, 3H), 1.37 (qd, J=11.00, 7.0 Hz, 1H).
Step 1: 2-(4-bromophenyl)octahydro-1H-pyrrolo[1,2-a][1,4]diazepine
To a 30 mL vial was added 1-bromo-4-iodobenzene (509 mg, 1.80 mmol), octahydro-1H-pyrrolo[1,2-a][1,4]diazepine (378 mg, 2.70 mmol), tris(dibenzylideneacetone)dipalladium (0) (49.4 mg, 0.054 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (94 mg, 0.162 mmol) and Cs2CO3 (1759 mg, 5.40 mmol). The vial was sealed with a cap and septum and then the reaction vessel was evacuated and backfilled with nitrogen. Toluene (4 mL) was added and the reaction vessel was evacuated and backfilled with nitrogen an additional time. The reaction was then heated at 100° C. for 16 h, concentrated onto Celite® and purified by flash silica gel chromatography (eluting with 100% CH2Cl2 to 95-5-1 CH2Cl2/MeOH/NH4OH) to give the product (161 mg, 30%) as a brown oil. LCMS: [M+H]+=297.07.
Step 2: 6-(2-amino-5-(4-(hexahydro-1H-pyrrolo[1,2-a][1,4]diazepin-2(3H)-yl)phenyl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one
In a microwave vial with magnetic stir bar was placed 6-(2-amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-3,4-dihydroisoquinolin-1(2H)-one (119 mg, 0.325 mmol), 2-(4-bromophenyl)octahydro-1H-pyrrolo[1,2-a][1,4]diazepine (80 mg, 0.271 mmol), [1,12-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (19.83 mg, 0.027 mmol), K3PO4 (0.417 mL, 0.542 mmol), DME (4 mL) and water (2 mL). The flask was sealed, then heated to 90° C. for 1 h in a microwave apparatus, loaded onto Celite® and purified by flash silica gel chromatography (eluting with 100% CH2Cl2 to 95-5-1 CH2Cl2/MeOH/NH4OH), followed by purification by reverse phase chromatography (C18 column, MeCN—H2O) to give the product (49 mg, 38%) as a tan solid. 1H NMR (500 MHz, DMSO-d6) δ 8.21 (d, J=2.3 Hz, 1H), 7.97-7.89 (m, 2H), 7.54 (d, J=2.4 Hz, 1H), 7.49 (dd, J=7.9, 1.5 Hz, 1H), 7.46 (s, 1H), 7.42 (d, J=8.8 Hz, 2H), 6.74 (d, J=8.9 Hz, 2H), 5.64 (s, 2H), 3.81 (dd, J=13.9, 2.1 Hz, 1H), 3.66 (dt, J=14.5, 5.5 Hz, 1H), 3.51-3.44 (m, 2H), 2.98-2.91 (m, 4H), 2.48-2.45 (m, 1H), 2.24 (q, J=8.9 Hz, 1H), 2.04 (td, J=12.0, 4.2 Hz, 1H), 1.98-1.88 (m, 2H), 1.81-1.72 (m, 1H), 1.72-1.58 (m, 2H), 1.45-1.35 (m, 1H); LCMS: [M+H]+=454.42.
A mixture of 6-(2-amino-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)-7-fluoro-3,4-dihydroisoquinolin-1(2H)-one (383 mg, 0.999 mmol), Cs2CO3 (814 mg, 2.499 mmol), 4-(4-bromophenyl)piperidine (200 mg, 0.833 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (73.1 mg, 0.100 mmol) in DME (10 mL) and water (2.0 mL) in a microwave vial was flushed with argon and heated in a microwave reactor at 85° C. for 2 h. The reaction mixture was then partitioned between brine and EtOAc, the organic phase was separated, the aqueous phase was extracted with CHCl3/IPA(4:1) (x 3), the combined organic layers were dried over Na2SO4 and concentrated onto Celite®. Purification using silica gel chromatography eluting with CH2Cl2 containing 0-7% MeOH and 0-0.7% NH4OH afforded the title compound as a beige solid (216 mg, 59%). 1H NMR: (500 MHz, DMSO-d6) δ 8.37-8.28 (m, 1H), 8.14-8.06 (m, 1H), 7.67-7.59 (m, 2H), 7.58-7.52 (m, 2H), 7.47-7.40 (m, 1H), 7.29-7.21 (m, 2H), 5.89-5.77 (m, 2H), 4.20-3.96 (m, 1H), 3.46-3.40 (m, 2H), 3.08-3.00 (m, 2H), 2.98-2.91 (m, 2H), 2.65-2.54 (m, 3H), 1.74-1.65 (m, 2H), 1.60-1.44 (m, 2H); LCMS: [M+H]+=417.55
A stock solution of 10 mM of test compound is prepared in DMSO. The compound plate was prepared by 3-fold and 9-point serial dilutions. Recombinant (1-346) HPK1 (h) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.33 mg/mL myelin basic protein, 10 mM MgAcetate and [gamma-33P]-ATP (specific activity and concentration as required). The reaction is initiated by The addition of the Mg/ATP mix. After incubation for 40 minutes at RT, the reaction is stopped by the addition of phosphoric acid to a concentration of 0.5%. 10 uL of the reaction is then spotted onto a P30 filtermat and washed four times for 4 minutes in 0.425% phosphoric acid and once in methanol prior to drying and scintillation counting. For more details of kinase assay protocols, see: Gao, Y. et. al.; Biochem J.451 (2): 313-328, 2013. Compounds of the application showed activity as inhibitors of HPK1 having IC50's in the following ranges: A: 0.1-10 nM; B: 11-100 nM; C: 101-1000 nM; D: >1000 nM. Specific ranges for exemplary compounds of Formula (I) are shown in Table 2.
Lck (h) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 250 uM KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [gamma-33P]-ATP (specific activity and concentration as required). The reaction is initiated by the addition of the Mg/ATP mix. After incubation for 40 minutes at RT, the reaction is stopped by the addition of phosphoric acid to a concentration of 0.5%. 10 uL of the reaction is then spotted onto a P30 filtermat and washed four times for 4 minutes in 0.425% phosphoric acid and once in methanol prior to drying and scintillation counting. For more details on the kinase assay procedures see: Gao, Y. et. al.; Biochem J., 451 (2): 313-328, 2013. Generally, and advantageously, compounds of the application showed significantly less inhibition of Lck compared with inhibition of HPK1 (see Table 2 where IC0o's are reported the following ranges: A: 0.1-10 nM; B: 11-100 nM; C: 101-1000 nM; D: >1000 nM for the compounds of Formula (I)).
In some embodiments the presence of a halo substituent on the amino pyridine ring provides compounds with improved selectivity for inhibition of HPK1 vs Lck (see, for example, Table 3 and Table 4). (c) Human Jurkat T Lymphocyte Anti-proliferation Assay
Assay principle: Jurkat cells are incubated with various concentrations of test compounds for 72 h, and cell proliferation/cytotoxicity is measured via detection of ATP production.
Literature: See Cree, I A et. al.; Toxicol In Vitro., 11 (5): 553-556, 1997 for additional information of ATP detection.
Assay Procedure: Jurkat cells (cultured in a-MEM media with 10% FBS) are seeded at 2000 cells/well (50 μL) in a 384-well well black culture plate (Perkin Elmer). Test compounds (in DMSO) are added to cells using the HP digital dispenser, and incubated at 37° C., 5% CO2 for 72 hours. ATP production is measured by adding 40 μL/well of ATPLite-1-Step reagent (Perkin Elmer), incubating for 5 min at RT with shaking followed by detection of luminescent signal using an Envision plate reader (Perkin Elmer). Data is normalized to untreated cells, and plotted using XLFit. IC50 values are calculated using a 4 parameter dose-response equation by fitting the curve of % inhibition versus Log of compound concentration.
Results: In an embodiment, exemplary compounds of the application had IC50's in the range of 0.1 to >10 μM in this assay. In an embodiment, exemplary compounds of the application had IC50's in the range of 1.0 to >10 uM in this assay.
Assay principle: Stimulation of TCR signaling via anti-CD3/CD28 antibody treatment in Jurkat T cells will lead to IL-2 secretion into the culture media, detected by a luminescent energy transfer bead immunoassay (IL-2 AlphaLISA kit).
Literature: See Cauchon, E. et. al.; Analytical Biochemistry., 388 (1): 134-139, 2009 for additional information on AlphaLISA detection.
Assay procedure: Jurkat cells (cultured in a-MEM media with 10% FBS) are seeded at 0.2×106 cells/well (100 μL) in a 96-well round bottom culture plate (Greiner). Test compounds (in DMSO) are added to cells using an HP digital dispenser, and incubated for 15 mins at RT. Cells are stimulated with 15 μL/mL (v/v) of soluble CD3/CD28 antibodies in a-MEM media (Stem Cell Technologies; 50 μL/well, 150 μL final assay volume), and incubated at 37° C., 5% CO2 for 4 hours. Cells are centrifuged at 1500 rpm for 5 mins at RT, and 5 μL of culture media is transferred to a 96 well % area white plate (Perkin Elmer). IL-2 is detected by adding 20 μL/well of a mixture of both Acceptor beads and Biotin anti-IL-2 antibody (1:200 dilution of each), and incubated for 1 hour at RT with shaking. 25 μL/well of Donor beads (1:63 dilution) are then added and incubated in the dark for 30 mins at RT with shaking, followed by detection of luminescent signal using an Envision plate reader (Perkin Elmer). Data is normalized to untreated/stimulated cells, and plotted using XLFit. EC50 values are calculated using a 4 parameter dose-response equation by fitting the curve of % stimulation versus Log of compound concentration.
Results: In an embodiment, exemplary compounds of the application had EC150's in the range of 0.01-5 uM in this assay. In an embodiment, exemplary compounds of the application had EC150's in the range of 0.01-1.3 μM in this assay.
(e) p-SLP76 S376 Phosphorylation Inhibition Assay
Assay principle: Stimulation of TCR signaling via anti-CD3/CD28 antibody treatment in Jurkat T cells will lead to phosphorylation of HPK1 at Serine 376, detected by a luminescent energy transfer bead immunoassay (p-SPL76 AlphaLISA kit).
Literature: See Cauchon, E. et. al.; Analytical Biochemistry., 388 (1): 134-139, 2009 for additional information on AlphaLISA detection.
Assay procedure: Jurkat cells (cultured in a-MEM media with 10% FBS) are collected and centrifuged, and seeded in a 96-well % area white plate (Perkin Elmer) at 0.1×106 cells/well in HBSS (25 μL). Test compounds (in DMSO) are added to cells using an HP digital dispenser, and incubated for 15 mins at RT. Cells are stimulated with 15 μl/mL (v/v) of soluble CD3/CD28 antibodies in HBSS (Stem Cell Technologies; 25 μL, 50 μL final assay volume), and incubated at 37° C., 5% CO2 for 1 hour. Cells are lysed with 10 μL/well of lysis buffer followed by incubation at RT for 10 mins with shaking. p-SLP76 S376 is detected by adding 15 μl/well of Acceptor beads (1:50 dilution), and incubated for 1 hour at RT with shaking. 15 μL/well of Donor beads (1:50 dilution) are then added and incubated at RT in the dark for 1 hour with shaking, followed by detection of luminescent signal using an Envision plate reader (Perkin Elmer). Data is normalized to untreated/stimulated cells, and plotted using Graph Pad Prism. IC50 values are calculated using a 4 parameter dose-response equation by fitting the curve of % inhibition versus Log of compound concentration. (e) Glucose kinase (GIk) Assay
GLK(h) (MAP4K3(h)) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 μM RLGRDKYKTLRQIRQ, 10 mM Magnesium acetate and [9-33P-ATP] (specific activity and concentration as required). The reaction is initiated by the addition of the Mg/ATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of phosphoric acid to a concentration of 0.5%. 10 μl of the reaction is then spotted onto a P30 filtermat and washed four times for 4 minutes in 0.425% phosphoric acid and once in methanol prior to drying and scintillation counting. For more details on the kinase assay procedures see: Gao, Y. et. al.; Biochem J., 451 (2): 313-328, 2013.
Results: In an embodiment, exemplary compounds of the application had IC50's in the range of greater than 10× greater than the value for HPK1 in this assay. In an embodiment, exemplary compounds of the application had IC50's in the range of at least 20× greater than HPK1. in this assay.
While the present application has been described with reference to what are presently considered to be the preferred examples, it is to be understood that the application is not limited to the disclosed examples. To the contrary, the present application is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.
All publications, patents and patent applications are herein incorporated by reference in their entirety to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety. Where a term in the present application is found to be defined differently in a document incorporated herein by reference, the definition provided herein is to serve as the definition for the term.
The present application claims the benefit of priority of co-pending U.S. provisional patent application No. 63/182,203 filed on Apr. 30, 2021, the contents of which are incorporated herein by reference in their entirety.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CA2022/050678 | 5/2/2022 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 63182203 | Apr 2021 | US |
