Claims
- 1. An oligonucleotide comprising a nucleic acid sequence specifically hybridizable with a polynucleotide sequence encoding a heparanase.
- 2. The oligonucleotide of claim 1, wherein said oligonucleotide sequence is at least 10 and no more than 650 nucleotides in length.
- 3. The oligonucleotide of claim 1, wherein said polynucleotide sequence encoding heparanase is as set forth by SEQ ID NO:1.
- 4. The oligonucleotide of claim 1, wherein said polynucleotide sequence encoding heparanase is encompassed by nucleotides 728-1351 of SEQ ID NO:1.
- 5. The oligonucleotide of claim 1, wherein said polynucleotide sequence is a messenger RNA of said heparanase.
- 6. The oligonucleotide of claim 1, wherein said oligonucleotide is labeled with a detectable moiety.
- 7. The oligonucleotide of claim 6, wherein said detectable moiety is selected from the group consisting of a chromogenic moiety, a fluorogenic moiety, a light-emitting moiety and a radioactive moiety.
- 8. The oligonucleotide of claim 1, wherein the oligonucleotide is selected from the group consisting of a synthetic oligonucleotide, an antisense heparanase RNA and heparanase DNA.
- 9. The oligonucleotide of claim 1, wherein said nucleic acid sequence includes natural nucleotides and/or nucleotide analogs.
- 10. A kit for detecting a polynucleotide sequence encoding heparanase, the kit comprising the oligonucleotide of claim 1 and reagents for detecting a hybridization between said oligonucleotide and the polynucleotide sequence encoding heparanase.
- 11. The kit of claim 10, wherein said oligonucleotide is labeled with a detectable moiety.
- 12. The kit of claim 10, wherein said detectable moiety is selected from the group consisting of a chromogenic moiety, a fluorogenic moiety, a light-emitting moiety and a radioactive moiety.
- 13. A method of detecting presence or absence or level of a polynucleotide sequence encoding heparanase in a biological sample, the method comprising:
(a) exposing the biological sample to an oligonucleotide, having a nucleic acid sequence specifically hybridizable with the polynucleotide sequence encoding the heparanase; and (b) detecting specific hybridization, thereby detecting presence, absence or level of the polynucleotide sequence encoding heparanase in the biological sample.
- 14. The method of claim 13, wherein said biological sample is a cell, a tissue sample or body fluid.
- 15. The method of claim 13, wherein said biological sample includes malignant cells.
- 16. The method of claim 13, wherein said biological sample is derived from solid tumor or a hematopoietic tumor.
- 17. The method of claim 16, wherein said solid tumor is selected from the group consisting of carcinoma, adenocarcinoma, squameous cell carcinoma, teratocarcinoma, mesothelioma, melanoma, renal cell carcinoma, myeloma, and rabdomyosarcoma and further wherein said hematopoietic tumor is selected from the group consisting of lymphoma and leukemia.
- 18. The method of claim 16, wherein said solid tumor is a primary tumor, or a metastasis thereof, and is originated from an organ selected from the group consisting of liver, prostate, bladder, breast, ovary, cervix, colon, skin, intestine, stomach, uterus, pancreas.
- 19. The method of claim 13, wherein said oligonucleotide is selected from the group consisting of a synthetic oligonucleotide, an antisense to heparanase RNA and heparanase DNA.
- 20. The method of claim 13, wherein said oligonucleotide is labeled with a detectable moiety.
- 21. The method of claim 20, wherein said detectable moiety is selected from the group consisting of a chromogenic moiety, a fluorogenic moiety, a light-emitting moiety and a radioactive moiety 22. The method of claim 13, wherein the polynucleotide sequence encoding the heparanase is mRNA and said oligonucleotide is designed for directing reverse transcription of said mRNA into cDNA and whereas step (b) is effected by polymerase chain reaction amplification of said cDNA.
Parent Case Info
[0001] This is a continuation of U.S. patent application Ser. No. 09/944,602, filed Sep. 4, 2001, which is a continuation of U.S. patent application Ser. No. 09/759,207, filed Jan. 16, 2001, which is a continuation of U.S. patent application Ser. No. 09/322,977, filed Jun. 1, 1999, now U.S. Pat. No. 6,531,129, issued Mar. 11, 2003, which is a divisional of U.S. patent application Ser. No. 09/071,739, filed May 1, 1998, now U.S. Pat. No. 6,177,545, issued Jan. 23, 2001, which is a continuation-in-part of U.S. patent application Ser. No. 08/922,170, filed Sep. 2, 1997, now U.S. Pat. No. 5,968,822, issued Oct. 19, 1999.
Divisions (1)
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Number |
Date |
Country |
Parent |
09071739 |
May 1998 |
US |
Child |
09322977 |
Jun 1999 |
US |
Continuations (3)
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Number |
Date |
Country |
Parent |
09944602 |
Sep 2001 |
US |
Child |
10851135 |
May 2004 |
US |
Parent |
09759207 |
Jan 2001 |
US |
Child |
09944602 |
Sep 2001 |
US |
Parent |
09322977 |
Jun 1999 |
US |
Child |
09759207 |
Jan 2001 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
08922170 |
Sep 1997 |
US |
Child |
09071739 |
May 1998 |
US |