Claims
- 1. Hepatosin, a compound having a molecular weight between 500 and 5000, resistant to heating at 100.degree. C. and having an amino acid composition of aspartic acid, threonine, glutamic acid, glycine, cystine, tyrosine, phenylalanine, and lysine, said hepatosin being capable of being obtained by culturing C.sub.3 H mouse liver cells by suspending 50 million liver cells in 50 ml of RPMI-1640 supplemented with 1% L-glutamine and free from fetal calf serum, antibiotics, and phenol red at 37.degree. C. for 24 hours in an atmosphere of 95% air, 5% CO.sub.2, followed by harvesting by centrifugation.
- 2. A method of preparing the hepatosin of claim 1 comprising culturing C.sub.3 H mouse liver cells by suspending 50 million liver cells ion the medium RPMI-1640 supplemented with L-glutamine and free from fetal calf serum, antibiotics and phenol red at 37.degree. C. for 24 hours in an atmosphere of 95% air, 5% CO.sub.2, followed by harvesting by centrifugation and recovering the hepatosin thus prepared.
- 3. A method according to claim 2 wherein the culture medium includes an immunopotentiator in an amount sufficient to enhance the yield of hepatosin.
- 4. A method according to claim 3 wherein the immunopotentiator is diethyldithiocarbamic acid or a salt thereof, levamisole of inosiflex.
- 5. A method according to claim 3 wherein the immunopotentiator is the sodium salt of diethyldithiocarbamic acid.
- 6. A composition containing the hepatosin of claim 1 in a concentration at least about 100 times as great as it is obtained by culturing C.sub.3 H mouse liver cells by suspending 50 million liver cells in 50 ml of RPMI-1640 supplemented with 1% L-glutamine and free from fetal calf serum, antibiotics and phenol red at 37.degree. C. for 24 hours in an atmosphere of 95% air, 5% CO.sub.2, followed by harvesting by centrifugation.
- 7. A liquid composition containing the hepatosin of claim 1 in an amount of at least 40 mg/ml.
- 8. A composition according to claim 7 which is an aqueous liquid composition.
- 9. A method of imparting immunomodulating activity or, antiviral activity or comprising administering to a mammal an effective amount for such puropose of the hepatosin of claim 1.
- 10. A method according to claim 9 of imparting immunomodulating activity comprising administering to a mammal an effective amount of such purpose of the hepatosin of claim 1.
- 11. A method according to claim 9 of imparting antiviral activity comprising administering to a mammal an effective amount of such purpose of the hepatosin of claim 1.
- 12. A liquid composition containing the hepatosin of claim 1 in an amount of at least 40 mg/ml.
- 13. A method of imparting immunomodulating activity or antiviral activity comprising administering to a mammal an effective amount for such purpose of the hepatosin of claim 1.
- 14. A method for reconstituting immune functions in a thymic deprived or immunodeprived mammal which method comprises administering to said mammal an immunopotentiating effective amount of the hepatosin claim 1.
- 15. A method for the treatment of a condition in a mammal resulting from relative or absolute T-cell deficiencies, which comprises administering a therapeutically effective amount of the hepatosin of claim 1.
- 16. A method for the treatment of a primary or secondary immunodeficiency in a mammal comprising administering to the mammal an effective amount for such purpose of the hepatosin of claim 1.
- 17. A method according to claim 15 of treating primary immunodeficiency.
- 18. A method according to claim 15 of treating secondary ummunodeficiency.
- 19. A method according to claim 9, wherein the dosage administered is 1-1000 .mu.g/kg body weight of the mammal.
Parent Case Info
This is a continuation of application Ser. No. 237,729, filed Feb. 24, 1981, now abandoned.
US Referenced Citations (7)
Continuations (1)
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Number |
Date |
Country |
Parent |
237729 |
Feb 1981 |
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