Claims
- 1. A method for producing a human Kunitz-type inhibitor, comprising the steps of:
- culturing a host cell containing a DNA construct comprising a first DNA segment encoding a human Kunitz-type inhibitor, wherein said Kunitz-type inhibitor comprises the amino acid sequence of SEQ ID NO:2 from alanine, amino acid 56 to alanine, amino acid number 110; the amino acid sequence of SEQ ID NO:2 from aspartic acid, amino acid number 53 to alanine, amino acid number 110; or the amino acid sequence of SEQ ID NO:2 from valine, amino acid number 57 to alanine, amino acid number 110 and which further contains a glutamic acid residue on the amino terminus, wherein said first DNA segment is operably linked to additional DNA segments required for the expression of said first DNA segment; and
- isolating said Kunitz-type inhibitor.
- 2. A method for producing a human Kunitz-type inhibitor according to claim 1, wherein said first DNA segment comprises the sequence of nucleotides of SEQ ID NO:1 from nucleotide 171 to nucleotide 331 or the sequence of nucleotides of SEQ ID NO:1 from nucleotide 159 to nucleotide 331.
- 3. The method of claim 1, wherein the host cell is selected from the group consisting of mammalian cells, yeast cells and E. coli.
- 4. A method for producing a human amyloid protein precursor homologue, comprising the steps of:
- culturing a host cell containing a DNA construct comprising a first DNA segment encoding a human amyloid protein precursor homologue, wherein said amyloid protein precursor homologue comprises the amino acid sequence of SEQ ID NO:13 from methionine, amino acid number 1, to isoleucine, amino acid number 763, wherein said first DNA segment is operably linked to additional DNA segments required for the expression of said first DNA segment; and
- isolating said amyloid protein precursor homologue.
- 5. A method for producing a human amyloid protein precursor homologue according to claim 4, wherein said first DNA segment comprises the sequence of nucleotides of SEQ ID NO:12 from nucleotide 73 to nucleotide 2361.
- 6. The method of claim 4, wherein the host cell is selected from the group consisting of mammalian cells, yeast cells and E. coli.
CROSS REFERENCE
This is a continuation application of application Ser. No. 08/155,331, filed Nov. 19, 1993, now U.S. Pat. No. 5,441,931 and Ser. No. 07/985,692 filed Dec. 2, 1992, now U.S. Pat. No. 5,436,153.
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Related Publications (1)
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985692 |
Dec 1992 |
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Continuations (1)
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155331 |
Nov 1993 |
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