HUMAN ANALOGS OF MURINE DEUBIQUITINATING PROTEASE

Abstract

The present invention is directed to human analogs of murine hematopoetic-specific, cytokine-inducible deubiquinating proteases (“DUBs”) clustered on chromosomes 4 and 8 and their respective regulatory regions. The nucleotides or proteins encoded thereby my be used in assays to identify inhibitors of human DUBs.

Description

BACKGROUND OF THE INVENTION

The role of ubiquitin in protein degradation was discovered and the main enzymatic reactions of this system elucidated in biochemical studies in a cell-free system from reticulocytes. In this system, proteins are targeted for degradation by covalent ligation to ubiquitin, a 76-amino-acid-residue protein. Briefly, ubiquitin-protein ligation requires the sequential action of three enzymes. The C-terminal Gly residue of ubiquitin is activated in an ATP-requiring step by a specific activating enzyme, E1 (Step 1). This step consists of an intermediate formation of ubiquitin adenylate, with the release of PP_i, followed by the binding of ubiquitin to a Cys residue of E1 in a thiolester linkage, with the release of AMP. Activated ubiquitin is next transferred to an active site Cys residue of a ubiquitin-carrier protein, E2 (Step 2). In the third step catalyzed by a ubiquitin-protein ligase or E3 enzyme, ubiquitin is linked by its C-terminus in an amide isopeptide linkage to an -amino group of the substrate protein's Lys residues (Step 3).

Proteins ligated to polyubiquitin chains are usually degraded by the 26S proteasome complex that requires ATP hydrolysis for its action. The 26S proteasome is formed by an ATP-dependent assembly of a 20S proteasome, a complex that contains the protease catalytic sites, with 19S “cap” or regulatory complexes. The 19S complexes contain several ATPase subunits and other subunits that are presumably involved in the specific action of the 26S proteasome on ubiquitinylated proteins. The roles of ATP in the assembly of the 26S proteasome complex and in its proteolytic action are not understood. The action of the 26S proteasome presumably generates several types of products: free peptides, short peptides still linked to ubiquitin via their Lys residues, and polyubiquitin chains (Step 4). The latter two products are converted to free and reusable ubiquitin by the action of ubiquitin-C-terminal hydrolases or isopeptidases (Steps 5 and 6). Some isopeptidases may also disassemble certain ubiquitin-protein conjugates (Step 7) and thus prevent their proteolysis by the 26S proteasome. The latter type of isopeptidase action may have a correction function to salvage incorrectly ubiquitinylated proteins or may have a regulatory role. Short peptides formed by the above processes can be further degraded to free amino acids by cytosolic peptidases (Step 8).

Ubiquitin-mediated degradation of protein is involved in various biological processes. The selective and programmed degradation of cell-cycle regulatory proteins, such as cyclins, inhibitors of cyclin-dependent kinases, and anaphase inhibitors are essential events in cell-cycle progression. Cell growth and proliferation are further controlled by ubiquitin-mediated degradation of tumor suppressors, protooncogenes, and components of signal transduction systems. The rapid degradation of numerous transcriptional regulators is involved in a variety of signal transduction processes and responses to environmental cues. The ubiquitin system is clearly involved in endocytosis and down-regulation of receptors and transporters, as well as in the degradation of resident or abnormal proteins in the endoplasmic reticulum. There are strong indications for roles of the ubiquitin system in development and apoptosis, although the target proteins involved in these cases have not been identified. Dysfunction in several ubiquitin-mediated processes causes pathological conditions, including malignant transformation.

Our knowledge of different signals in proteins that mark them for ubiquitinylation is also limited. Recent reports indicate that many proteins are targeted for degradation by phosphorylation. It was observed previously that many rapidly degraded proteins contain PEST elements, regions enriched in Pro, Glu, Ser, and Thr residues. More recently, it was pointed out that PEST elements are rich in S/TP sequences, which are minimum consensus phosphorylation sites for Cdks and some other protein kinases. Indeed, it now appears that in several (though certainly not all) instances, PEST elements contain phosphorylation sites necessary for degradation. Thus multiple phosphorylations within PEST elements are required for the ubiquitinylation and degradation of the yeast G1 cyclins Cln3 and Cln2, as well as the Gcn4 transcriptional activator. Other proteins, such as the mammalian G1 regulators cyclin E and cyclin D1, are targeted for ubiquitinylation by phosphorylation at specific, single sites. In the case of the IkBα inhibitor of the NF-kB transcriptional regulator, phosphorylation at two specific sites, Ser-32 and Ser-36, is required for ubiquitin ligation. β-cateinin, which is targeted for ubiquitin-mediated degradation by phosphorylation, has a sequence motif similar to that of IkBα around these phosphorylation sites. However, the homology in phosphorylation patterns of these two proteins is not complete, because phosphorylation of other sites of β-cateinin is also required for its degradation. Other proteins targeted for degradation by phosphorylation include the Cdk inhibitor Siclp and the STAT1 transcription factor. Though different patterns of phosphorylation target different proteins for degradation, a common feature appears to be that the initial regulatory event is carried out by a protein kinase, while the role of a ubiquitin ligase would be to recognize the phosphorylated form of the protein substrate. It further appears that different ubiquitin ligases recognize different phosphorylation patterns as well as additional motifs in the various protein substrates. However, the identity of such E3s is unknown, except for some PULC-type ubiquitin ligases that act on some phosphorylated cell-cycle regulators in the budding yeast. The multiplicity of signals that target proteins for ubiquitin-mediated degradation (and of ligases that have to recognize such signals) is underscored by observations that the phosphorylation of some proteins actually prevents their degradation. Thus the phosphorylation of the c-Mos protooncogene on Ser3 and the multiple phosphorylations of c-Fos and c-Jun protooncogenes at multiple sites by MAP kinases suppress their ubiquitinylation and degradation.

In addition to the families of enzymes involved in conjugation of ubiquitin, a very large family of deubiquitinating enzymes has recently been identified from various organisms. These enzymes have several possible functions. First, they may have peptidase activity and cleave the products of ubiquitin genes. Ubiquitin is encoded by two distinct classes of genes. One is a polyubiquitin gene, which encodes a linear polymer of ubiquitins linked through peptide bonds between the C-terminal Gly and N-terminal Met of contiguous ubiquitin molecules. Each copy of ubiquitin must be released by precise cleavage of the peptide bond between Gly-76-Met-1 of successive ubiquitin moieties. The other class of ubiquitin genes encodes ubiquitin C-terminal extension proteins, which are peptide bond fusions between the C-terminal Gly of ubiquitin and N-terminal Met of the extension protein. To date, the extensions described are ribosomal proteins consisting of 52 or 76-80 amino acids. These ubiquitin fusion proteins are processed to yield ubiquitin and the corresponding C-terminal extension proteins. Second, deubiquitinating enzymes may have isopeptidase activities. When a target protein is degraded, deubiquitinating enzymes can cleave the polyubiquitin chain from the target protein or its remnants. The polyubiquitin chain must also be disassembled by deubiquitinating enzymes during or after proteolysis by the 26 S proteasome, regenerating free monomeric ubiquitin. In this way, deubiquitinating enzymes can facilitate the ability of the 26 S proteasome to degrade ubiquitinated proteins. Third, deubiquitinating enzymes may hydrolyze ester, thiolester, and amide linkages to the carboxyl group of Gly-76 of ubiquitin. Such nonfunctional linkages may arise from reactions between small intracellular compounds such as glutathione and the E1-, E2-, or E3-ubiquitin thiolester intermediates. Fourth, deubiquitinating enzymes may compete with the conjugating system by removing ubiquitin from protein substrates, thereby rescuing them from degradation or any other function mediated by ubiquitination. Thus generation of ubiquitin by deubiquitinating enzymes from the linear polyubiquitin and ubiquitin fusion proteins and from the branched polyubiquitin ligated to proteins should be essential for maintaining a sufficient pool of free ubiquitin. Many deubiquitinating enzymes exist, suggesting that these deubiquitinating enzymes recognize distinct substrates and are therefore involved in specific cellular processes. Although there is recent evidence to support such specificity of these deubiquitinating enzymes, the structure-function relationships of these enzymes remain poorly studied.

Deubiquitinating enzymes can be divided broadly on the basis of sequence homology into two classes, the ubiquitin-specific processing protease (UBP or USP, also known as type 2 ubiquitin C-terminal hydrolase (type 2 UCH)) and the UCH, also known as type 1 UCH). UCH (type 1 UCH) enzymes hydrolyze primarily C-terminal esters and amides of ubiquitin but may also cleave ubiquitin gene products and disassemble polyubiquitin chains. They have in common a 210-amino acid catalytic domain, with four highly conserved blocks of sequences that identify these enzymes. They contain two very conserved motifs, the CYS and HIS boxes. Mutagenesis studies revealed that the two boxes play important roles in catalysis. Some UCH enzymes have significant C-terminal extensions. The functions of the C-terminal extensions are still unknown but appear to be involved in proper localization of the enzyme. The active site of these UCH enzymes contains a catalytic triad consisting of cysteine, histidine, and aspartate and utilizes a chemical mechanism similar to that of papain. The crystal structure of one of these, UCH-L3, has been solved at 1.8 Å resolution. The enzyme comprises a central antiparallel β-sheet flanked on both sides by helices. The β-sheet and one of the helices are similar to those observed in the thiol protease cathepsin B. The similarity includes the three amino acid residues that comprise the active site, Cys⁹⁵, His¹⁶⁹, and Asp¹⁸⁴. The active site appears to fit the binding of ubiquitin that may anchor also at an additional site. The catalytic site in the free enzyme is masked by two different segments of the molecule that limit nonspecific hydrolysis and must undergo conformational rearrangement after substrate binding.

UBP (type 2 UCH) enzymes are capable of cleaving the ubiquitin gene products and disassembling polyubiquitin chains after hydrolysis. It appears that there is a core region of about 450 amino acids delimited by CYS and HIS boxes. Many of these isoforms have N-terminal extensions and a few have C-terminal extensions. In addition, there are variable sequences in the core region of many of the isoforms. The functions of these divergent sequences remain poorly characterized. Another interesting function of specific UBPs is the regulation of cell proliferation. It was observed that cytokines induced in T-cells specific de-ubiquitinating enzymes (DUBs), termed DUB-1 and DUB-2. DUB-1 is induced by stimulation of the cytokine receptors for IL-3, IL-5, and GM-CSF, suggesting a role in its induction for the β-common (betac) subunit of the interleukin receptors. Overexpression of a dominant negative mutant of JAK2 inhibits cytokine induction of DUB-1, suggesting that the regulation of the enzyme is part of the cell response to the JAK/STAT signal transduction pathway. Continued expression of DUB-1 arrests cells at G₁; therefore, the enzyme appears to regulate cellular growth via control of the G₀-G₁ transition. The catalytic conserved Cys residue of the enzyme is required for its activity. DUB-2 is induced by IL-2 as an immediate early (IE) gene that is down-regulated shortly after the initiation of stimulation. The function of this enzyme is also obscure. It may stimulate or inhibit the degradation of a critical cell-cycle regulator.

Cytokines, such as interleukin-2 (IL-2), activate intracellular signaling pathways via rapid tyrosine phosphorylation of their receptors, resulting in the activation of many genes involved in cell growth and survival. The deubiquitinating enzyme DUB-2 is induced in response to IL-2 and is expressed in human T-cell lymphotropic virus-I (HTLV-1)-transformed T cells that exhibit constitutive activation of the IL-2 JAK/STAT (signal transducers and activators of transcription) pathway, and when expressed in Ba/F3 cells DUB-2 markedly prolonged IL-2-induced STAT5 phosphorylation. Although DUB-2 does not enhance IL-2-mediated proliferation, when withdrawn from growth factor, cells expressing DUB-2 had sustained STAT5 phosphorylation and enhanced expression of IL-2-induced genes cis and c-myc. DUB-2 expression markedly inhibited apoptosis induced by cytokine withdrawal allowing cells to survive. Therefore, DUB-2 has a role in enhancing signaling through the JAK/STAT pathway, prolonging lymphocyte survival, and, when constitutively expressed, may contribute to the activation of the JAK/STAT pathway observed in some transformed cells. (Migone, T.-S., et al., Blood. 2001; 98:1935-1941).

Protein ubiquitination is an important regulator of cytokine-activated signal transduction pathways and hematopoietic cell growth. Protein ubiquitination is controlled by the coordinate action of ubiquitin-conjugating enzymes and deubiquitinating enzymes. Recently a novel family of genes encoding growth-regulatory deubiquitinating enzymes (DUB-1 and DUB-2) has been identified. DUBs are immediate-early genes and are induced rapidly and transiently in response to cytokine stimuli. By means of polymerase chain reaction amplification with degenerate primers for the DUB-2 complementary DNA, 3 murine bacterial artificial chromosome (BAC) clones that contain DUB gene sequences were isolated. One BAC contained a novel DUB gene (DUB-2A) with extensive homology to DUB-2. Like DUB-1 and DUB-2, the DUB-2A gene consists of 2 exons. The predicted DUB-2A protein is highly related to other DUBs throughout the primary amino acid sequence, with a hypervariable region at its C-terminus. In vitro, DUB-2A had functional deubiquitinating activity; mutation of its conserved amino acid residues abolished this activity. The 5′ flanking sequence of the DUB-2A gene has a hematopoietic-specific functional enhancer sequence. It is proposed that there are at least 3 members of the DUB subfamily (DUB-1, DUB-2, and DUB-2A) and that different hematopoietic cytokines induce specific DUB genes, thereby initiating a cytokine-specific growth response. (Baek, K.-H., et al, Blood. 2001; 98:636-642).

Protein ubiquitination also serves regulatory functions in the cell that do not involve proteasome-mediated degradation. For example, Hicke and Riezman have recently demonstrated ligand-inducible ubiquitination of the Ste2 receptor in yeast. Ubiquitination of the Ste2 receptor triggers receptor endocytosis and receptor targeting to vacuoles, not proteasomes. Also, Chen et al. have demonstrated that activation of the IB kinase requires a rapid, inducible ubiquitination event. This ubiquitination event is a prerequisite for the specific phosphorylation of IB and does not result in subsequent proteolysis of the kinase complex. The ubiquitination of Ste2 and IB kinase appears reversible, perhaps resulting from the action of a specific deubiquitinating enzyme.

A large superfamily of genes encoding deubiquitinating enzymes, or UBPs, has recently been identified. UBPs are ubiquitin-specific thiol-proteases that cleave either linear ubiquitin precursor proteins or post-translationally modified proteins containing isopeptide ubiquitin conjugates. The large number of UBPs suggests that protein ubiquitination, like protein phosphorylation, is a highly reversible process that is regulated in the cell.

Interestingly, UBPs vary greatly in length and structural complexity, suggesting functional diversity. While there is little amino acid sequence similarity throughout their coding region, sequence comparison reveals two conserved domains. The Cys domain contains a cysteine residue that serves as the active enzymatic nucleophile. The His domain contains a histidine residue that contributes to the enzyme's active site. More recent evidence demonstrates six homology domains contained by all members of the ubp superfamily. Mutagenesis of conserved residues in the Cys and His domains has identified several residues that are essential for UBP activity.

Recently, a growth regulatory deubiquitinating enzyme, DUB-1, that is rapidly induced in response to cytokine receptor stimulation was identified. DUB-1 is specifically induced by the receptors for IL-3, granulocyte macrophage-colony-stimulating factor, and IL-5, suggesting a specific role for the c subunit shared by these receptors. In the process of cloning the DUB-1 gene, a family of related, cross-hybridizing DUB genes was identified. From this, other DUB genes might be induced by different growth factors. Using this approach, an IL-2-inducible DUB enzyme, DUB-2 and closely related DUB-2a were identified. DUB-1 and DUB-2 are more related to each other than to other members of the ubp superfamily and thereby define a novel subfamily of deubiquitinating enzymes.

Hematopoietic-specific, cytokine induced DUBs in murine system have shown to prolong cytokine receptor, see Migone, T. S., et al. (2001). The deubiquitinating enzyme DUB-2 prolongs cytokine-induced signal transducers and activators of transcription activation and suppresses apoptosis following cytokine withdrawal, Blood 98, 1935-41; Zhu, Y., et al., (1997). DUB-2 is a member of a novel family of cytokine-inducible deubiquitinating enzymes, J Biol Chem 272, 51-7 and Zhu, Y., et al., (1996). The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor, Mol Cell Biol 16, 4808-17.). These effects are likely due to the deubiquitination of receptors or other signaling intermediates by DUB-1 or DUB-2, murine analogs of hDUBs. Inhibition of hDUBs may achieve downregulation of specific cytokine receptor signaling, thus modulating specific immune responses.

Cytokines regulate cell growth by inducing the expression of specific target genes. A recently identified a cytokine-inducible, immediate-early gene, DUB-1, encodes a deubiquitinating enzyme with growth regulatory activity. In addition, a highly related gene, DUB-2, that is induced by interleukin-2 was identified. The DUB-2 mRNA was induced in T cells as an immediate-early gene and was rapidly down-regulated. Like DUB-1, the DUB-2 protein had deubiquitinating activity in vitro. When a conserved cysteine residue of DUB-2, required for ubiquitin-specific thiol protease activity, was mutated to serine (C60S), deubiquitinating activity was abolished. DUB-1 and DUB-2 proteins are highly related throughout their primary amino acid sequence except for a hypervariable region at their COOH terminus. Moreover, the DUB genes co-localize to a region of mouse chromosome 7, suggesting that they arose by a tandem duplication of an ancestral DUB gene. Additional DUB genes co-localize to this region, suggesting a larger family of cytokine-inducible DUB enzymes. We propose that different cytokines induce specific DUB genes. Each induced DUB enzyme thereby regulates the degradation or the ubiquitination state of an unknown growth regulatory factor, resulting in a cytokine-specific growth response.

On the basis of these structural criteria, additional members of the DUB subfamily can be identified in the GenBank™. The highest degree of homologyis in the Cys and His domains. Additionally, this putative human DUB protein contains a Lys domain (amino acids 400-410) and a hypervariable region (amino acids 413-442).

Murine DUB (mDUB) subfamily members differ from other UBPs by functional criteria as well. mDUB subfamily members are cytokine-inducible, immediate-early genes and may therefore play regulatory roles in cellular growth or differentiation. Also, DUB proteins are unstable and are rapidly degraded by ubiquitin-mediated proteolysis shortly after their induction.

mDUB reports demonstrate that specific cytokines, such as IL-2 and IL-3, induce specific deubiquitinating enzymes (DUBs). The DUB proteins may modify the ubiquitin-proteolytic pathway and thereby mediate specific cell growth or differentiation signals. These modifications are temporally regulated. The DUB-2 protein, for instance, is rapidly but transiently induced by IL-2. Interference of DUB enzymes with specific isopeptidase inhibitors may block specific cytokine signaling events.

Defensins constitute a major family of antimicrobial peptides in mammals. Depending on the distribution of the cysteines and the linkages of the disulfide bonds, human defensins can be divided into two categories: α-defensins, which can be found in granulocytes and in epithelial cells of the small intestine, and β-defensins, which are expressed by epithelial cells and leukocytes including macrophages. Some defensins are expressed constitutive manner in granulocytes and epithelial cells where as others are induces by either exposure to microbial pathogens or pro-inflammatory cytokines such as IL-1β, TNF-α and interferon-γ. The genes coding for human defensins are clustered within 1 Mb segment on chromosome 8P23, and it has been suggested that β-defensins may predate the a-defensin family during recent gene amplification since α-defensin cannot be detected even in many mammalians including cow. Cow has at least 13 β-defensins but no α-defensin. β-defensins contribute to early host defense against several bacterial and fungal pathogens, as an important mechanism of innate immune response. Beside this antimicrobial activity, a chemoattractant activity on both immature dentritic cells and memory T cells, as well as monocytes, has been recently described, demonstrating that β-defensins may promote both innate and adaptive immune response.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1: Proposed enzyme reaction mechanism of DUB-

SUMMARY OF THE INVENTION

The present invention is directed to analogs of murine DUBs, hematopoietic-specific, cytokine-inducible deubiquitinating proteases found as a cluster of genes on chromosomes 4 and 8 and respective regulatory regions. Eleven novel human DUBs and four potential genes that express truncated form of DUBs not previously reported in public databases were identified by searching human genome database using murine DUB-1 and DUB-2 sequences. These genes share open reading frames (ORFs) that are 88 to 99% amino acid identity to each other, when gaps caused by deletion and N-terminal and/or C-terminal extension was not counted as mismatch, and exhibit approximately 50% identity to murine DUBs. Eight of eleven ORFs generate a protein of 530 amino acids. Two ORFs (hDUB8.3 and hDUB8.11) have internal in-frame deletions such that the genes are capable of generating 497 and 417 amino acid long polypeptides, respectively. One ORF (hDUB4.5) exhibits extension at both 5′ and 3′ end of the ORF so that the gene is capable of expressing 574 amino acid long polypeptide. Surprisingly, this 5′ extension results in specific pro-polypeptide sequence that can direct polypeptide targeting to the mitochondria. Furthermore, the respective regulatory regions, putative promoters, of these genes also share close to 90% identity each other suggesting that their expression is coordinated. In addition, we found that two of these genes can be expressed under the control of separate promoters that can be controlled independently and expressing potentially distinctive protein products.

Manipulation of these gene products by small molecular compounds can (1) reduce inflammation by regulating proinflammatory cytokine signaling, (2) modulate autoimmune diseases by regulating cytokine receptor signaling that are critical for lymphocytes proliferation, and (3) immune over-reaction during infection using above mechanisms.

Two of cluster genes (hDUB4.1 and hDUB4.2) possesses two distinctive promoter domains in front of their ORFs such that they can be regulated independently in their transcription potential. The longer transcripts of these ORFs (called hDUB4.1a and hDUB4.2a) has 12 and 4 exons respectively and capable of generating 1016 and 1021 amino acid long polypeptides, respectively. These polypeptides share C-terminal 530 amino acids with their shorter form that can be expressed separately from independent promoters (called hDUB4.1b and hDUB4.2b, respectively). In addition, two other ORFs are capable of generating longer than 530 amino acid polypeptides (hDUB4.10 and hDUB4.11). Remarkably, these two deduced polypeptides shares significant homology within portion of N-terminal portions (I added alignment file of these at the end of sequence file). Three of the ORFs (hDUB4.5, 4.8, and 8.2) has N-terminal insertion that is typical for mitochondria targeting sequence. An alignment of these sequences is provide in the Tables. The promoter sequences defined as upstream of initiation ATG of the ORF exhibit remarkable level of homology each other except that of hDUB4.1a. The sequence identity among all promoter sequences except that of hDUB4.1a is approximately 90% in 2000 base pair span upstream of initiation ATG. Two of the promoter sequences (hDUB8.3 and 8.11) have 334 nucleotides insertion at approximately 1000 base pair upstream of initiation ATG. Interestingly, hDUB8.3 and hDUB8.11 are the only ones with shorter ORFs due to the internal deletions. In addition to these ORFs, there are 5 ORFs that are capable of expressing polypeptides (hDUB4.4, hDUB4.9, hDUB8.2, hDUB8.9, and hDUB8.10) that share initiation codon with other 530 amino acid long polypeptides but terminate prematurely due to the in frame termination sequences. These also shares significant homology upstream of ATG initiation codon suggesting they may expressed as truncated proteins, potential regulatory functions. All 11 hDUB8 genes are clustered with the defensin clusters within 2 Mb region in 8P23, implying that both acquisition and amplification are relatively recent event, perhaps during mammalian evolution. It is of interest that hDUB4 gene cluster is also in highly amplified cluster region of chromosome 4P16 that is yet to be assigned in chromosome location. These data suggest that hDUB4s and hDUB8s are within very dynamic region of the human chromosomes (both 4p 16 and 8p23) that are undergoing volatile amplifications. The data also suggest that expression of hDUB8 may also be coordinated in conjunction with defensins that are critical components of innate immune response and inflammation.

Search Methods for Identifying Human Analogs of mDUBs:

In order to identify human analogs of mDUB1, -2, -2A, mDUB1 (U41636), mDUB2 (NM_—010089), and mDUB2A (AF393637) DNA sequences were used to search against Ensembl entire “golden path” (as contigs) using Ensembl blast search engine (http://www.ensembl.org/perl/blastview). All three mDUBs have significant alignments with contig AC083981, AF252831, AF228730, AF252830, AC068974 on chromosome 8 with the high score above 2000 and the probability less than e-87. In order to find all the homolog genes in the genome, exhaust search was performed using genomic aligned sequence to search against the “golden path” contigs. Two more contigs were found to have significant alignment that has probability less than e-100: one is AC074340 on chromosome 8 and the other is AC022770 on chromosome 4.

DNA sequences for contig AC083981, AF252831, AF228730, AF252830, AC068974, AC074340 and AC022770 were downloaded from Ensembl and gene annotation for each contig was performed using GenScan gene annotation program. Genes having homolog with mDUBs were named in sequence based on their locations on chromosomes.

For example, hDUB8.1 was derived from AF228730, 8.2, 8.3 were derived from AF252830, 8.5 were derived from AC074340, 8.6 were derived from AF252831, 8.7, 8.8 and 8.9 were derived from AC083981, and 8.10 and 8.11 were derived from AC068974. hDUB4.1, 4.2, 4.3, 4.4, 4.5 were derived from AC022770 on chromosome 4.

Using these hDUB4s and hDUB8s, both Ensemble and NCBI blast search was performed. Further contig NT_—028165 that covers chromosome 4 was identified. From this and already assembled chromosome 4p16.1 region, further annotation was performed using GenScan gene annotation program. From this we identified hDUB4.6, 4.7, 4.8, 4.9, 4.10, and 4.11.

Analysis of the hDUB gene clusters in chromosome 4 reveals that at least five ORFs in an unmapped cOntig (AC022770) were identified by nucleotide homology search with murine DUB1 and 2. At least four out of five ORFs share core 530 amino acid sequences. Two ORFs (hDUB4.1 and hDUB4.2) are multi-exon ORFs that extend N-terminal part of polypeptides that shares minimal sequence identity. However, there is a conserved putative promoter sequences that encompass over 2,000 bases in the intron region proximal to the last exon that is conserved among all 5 genes. Three of the ORFs (hDUB4.5, 4.8, and 8.2) has N-terminal insertion that is typical for mitochondria targeting sequence. The hDUB genes cluster in 4P16 of the human chromosome, which is an unmapped part of the human chromosome.

Analysis of the hDUB gene clusters in chromosome 8 reveals that at least eleven ORFs in six different contigs (AC068974, AC074340, AC083981, AF228730, AF252830, and AF252831) were identified by nucleotide homology search with murine DUB1 and 2. At least seven out of eleven ORFs share significant identities with similar length. There are conserved putative promoter sequences that encompass over 2,000 bases in all 11 genes. The hDUB genes cluster in 8P23.1 of the human chromosome and clustered with defensin molecules (at lease 9 defensins are clustered with hDUB8s) and the whole domain belongs the olfactory GPCR cluster.

Analysis of the deduced amino acid sequences of the hDUBs reveals polypeptides consistent with mDUBs, which contain highly conserved Cys and His domains that are likely to form the enzyme's active site. The putative active site nucleophile of mDUB-2 is a cysteine residue (Cys⁻⁶⁰) in the Cys domain. Both mDUB-1 and mDUB-2 have a lysine rich region (Lys domain; amino acids 374-384 of mDUB-2) and a short hypervariable region (amino acids 385-451 of mDUB-2), in which the mDUB-1 and mDUB-2 sequences diverge considerably. The hypervariable (HV) region of mDUB-2 contains a duplication of the eight-amino acid sequence: PQEQNHQK (Seq ID No. 55).

The protein and nucleotide sequences named in this invention and their corresponding Sequence ID No are set forth as following:

Nucleotide Sequence for hDUB4.1a: Seq ID No. 3

hDUB4.1a deduced polypeptide sequence: Seq ID No. 4

Nucleotide Sequence for hDUB4.1b: Seq ID No. 5

hDUB4.1b deduced polypeptide sequence: Seq ID No. 6

Nucleotide Sequence for hDUB4.2a: Seq ID No. 7

hDUB4.2a deduced polypeptide sequence: Seq ID No. 8

Nucleotide Sequence for hDUB4.2b: Seq ID No. 9

hDUB4.2b deduced polypeptide sequence: Seq ID No. 10

Nucleotide Sequence for hDUB4.3: Seq ID No. 11

hDUB4.3 deduced polypeptide sequence: Seq ID No. 12

Nucleotide Sequence for hDUB4.5: Seq ID No. 13

hDUB4.5 deduced polypeptide sequence: Seq ID No. 14

Nucleotide Sequence for hDUB4.6: Seq ID No. 15

hDUB4.6 deduced polypeptide sequence: Seq ID No. 16

Nucleotide Sequence for hDUB4.7: Seq ID No. 17

hDUB4.7 deduced polypeptide sequence: Seq ID No. 18

Nucleotide Sequence for hDUB4.8: Seq ID No. 19

hDUB4.8 deduced polypeptide sequence: Seq ID No. 20

Nucleotide Sequence for hDUB4.10: Seq ID No. 21

hDUB4.10 deduced polypeptide sequence: Seq ID No. 22

Nucleotide Sequence for hDUB4.11: Seq ID No. 23

hDUB4.11 deduced polypeptide sequence: Seq ID No. 24

Nucleotide Sequence for hDUB8.1: Seq ID No. 25

hDUB8.1 deduced polypeptide sequence: Seq ID No. 26

Nucleotide Sequence for hDUB8.3: Seq ID No. 27

hDUB8.3 deduced polypeptide sequence: Seq ID No. 28

Nucleotide Sequence for hDUB8.5: Seq ID No. 29

hDUB8.5 deduced polypeptide sequence: Seq ID No. 30

Nucleotide Sequence for hDUB8.6: Seq ID No. 31

hDUB8.6 deduced polypeptide sequence: Seq ID No. 32

Nucleotide Sequence for hDUB8.7: Seq ID No. 33

hDUB8.7 deduced polypeptide sequence: Seq ID No. 34

Nucleotide Sequence for hDUB8.8: Seq ID No. 35

hDUB8.8 deduced polypeptide sequence: Seq ID No. 36

Nucleotide Sequence for hDUB8.11: Seq ID No. 37

hDUB8.11 deduced polypeptide sequence: Seq ID No. 38

Nucleotide Sequence for hDUB4.4: Seq ID No. 39

hDUB4.4 deduced polypeptide sequence: Seq ID No. 40

Nucleotide Sequence for hDUB4.9: Seq ID No. 41

hDUB4.9 deduced polypeptide sequence: Seq ID No. 42

Nucleotide Sequence for hDUB8.2: Seq ID No. 43

hDUB8.2 deduced polypeptide sequence: Seq ID No. 44

Nucleotide Sequence for hDUB8.9: Seq ID No. 45

hDUB8.9 deduced polypeptide sequence: Seq ID No. 46

Nucleotide Sequence for hDUB8.10: Seq ID No. 47

hDUB8.10 deduced polypeptide sequence: Seq ID No. 48

Promoter sequence for hDUB4.6: Seq ID No. 49

Promoter sequence for hDUB4.7: Seq ID No. 50

Promoter sequence for hDUB4.8: Seq ID No. 51

Promoter sequence for hDUB4.9: Seq ID No. 52

Promoter sequence for hDUB4.10: Seq ID No. 53

Promoter sequence for hDUB4.11: Seq ID No. 54

TaqMan Real Time PCR Analysis of Expression of hDUB4s and hDUB8s in Human Immunocytes Upon Various Stimulation

Protocol of reverse transcription (RT) from total cellular RNA using random hexamer as primer (using TaqMan Reverse Transcription Reagents Cat# N808-0234)

1 ug of total RNA preparation in 100 ul of 1×TaqMan RT Buffer Mix, 5.5 mM MgCl₂, 0.5 mM dNTPs, 2.5 uM Random Hexamers, 40 U RNAse inhibitor, 125U Multiscribe Reverse Transcriptase. Mix by pipeting up and down. Incubate 25° C. for 10 minutes (annealing step), 48° C. for 30 minutes (reverse transcription), and 95° C. for 5 minutes (heat killing of the enzyme). The samples can be left at the machine at 4° C., or alternatively, can be stored at −20° C. Yield of cDNA synthesis can be measured by incorporation of small portion of radioactive dATP (or dCTP). Average efficiency for this protocol is between 60-80% of conversion of RNA to cDNA.

Protocol of TaqMan Real-Time Quantitative PCR

1 ul of TaqMan RT product in 12.5 ul of 1× master Mix (Applied Biosystems Cat# 4304437) containing all necessary reaction components except primers and probes, 0.9 uM forward primer, 0.9 uM reverse primer, 0.2 uM probe. Mix by pipetting up and down. Samples containing GADPH primer pair and probe were also prepared as control. Thermal cycling and detection of the real-time amplification were performed using the ABI PRISM 7900HT Sequence Detection System. The quantity of target gene is given relative to the GADPH control based on C_t values determined during the exponential phase of PCR.

Primer-Probe Sets Used and their Specificities:

Primer 4.1 is unique for hDUB 4.1

Primer 4.2 covers hDUB 4.2, 4.3, 4.5 and 8.1

Primer 8.3 covers hDUB 8.3 and 8.11

Primer 8.5 is unique for hDUB 8.5

Primer 8.6 covers hDUB 8.6, 8.7 and 8.8

TABLE 1
Expression of hDUBs in PBMC stimulated with LPS (100 ng/ml) and
PHA (5 μg/ml) for 7 hours.
	Donor 1
	Fold-		Donor 2
	Upregulation	Relative	Fold-Upregulation	Relative
Primer	upon stimulation	expression	upon stimulation	expression
4.1	2.2	1	3.8	1
4.2	2.0	21000	2.0	16400
8.3	1.8	5560	1.8	5500
8.5	2.1	80	3.1	310
8.6	2.6	19200	3.0	23000

TABLE 2
Expression of hDUBS in PBMC stimulated with LPS
(100 ng/ml) for 1.5, 7 and 24 hours (DONOR 4)
	1.5 hours	7 hours	24 hours
	Fold-		Fold-		Fold-
	Upregulation		Upregulation		Upregulation
	upon	Relative	upon	Relative	upon	Relative
Primer	stimulation	expression	stimulation	expression	stimulation	expression
4.2	2.4	64	336	35.2	1.8	12.5
8.3	0.4	1	13.1	1	1.7	1
8.5	1.6	11	65.9	4.8	1.5	1.8

TABLE 3
Expression of DUBs in PBMC stimulated with LPS (100 ng/ml)
and/or PHA (5 ug/ml) for 1.5, 7, 24 hours (donor 4)
	1.5 hours	7 hours	24 hours
	Fold-		Fold-		Fold-
	Upregulation		Upregulation		Upregulation
	upon	Relative	upon	Relative	upon	Relative
Stimuli	stimulation	expression	stimulation	expression	stimulation	expression
LPS	4.2	0.4	39	2.5	54	1.6	48
	8.3	0.5	5	1.6	6	1.1	7
	8.5	0.9	1	1.5	1.7	1.4	2
	8.6	0.6	26	1.7	57	1.0	21
PHA	4.2	3.5	367	4.4	94	0.9	26
	8.3	1.5	13	1.7	6	0.7	5
	8.5	1.9	2	0.9	1	0.7	1
	8.6	2.3	103	2.5	23	0.8	17
LPS + PHA	4.2	1.2	129	3.4	73	0.8	23
	8.3	1.0	9	2.2	8	0.7	5
	8.5	1.0	1	0.9	1.3	0.9	1.2
	8.6	1.3	56	2.5	33	0.8	18

There is no increase of expression in T lymphocytes (donor 5) and B lymphocytes (donor 6) when stimulated with anti-CD4/CD28 and anti-CD40/IL-4, respectively.

TABLE 4
Expression of hDUB 4.2, 4.3, 4.5 and 8.1 examined by primer 4.2 in
different human organ panel by TaqMan analysis.
			β2
	Tissue Type	Mean	Mean	∂∂ Ct	Expression
	Adrenal Gland	29.72	20.00	10.08	0.92
	Bone marrow	34.02	20.49	13.89	0.07
	Brain	26.92	22.73	4.54	42.84
	Colon	32.03	19.97	12.42	0.18
	Fetal Brain	27.59	24.23	3.71	76.15
	Fetal Liver	33.22	22.58	10.99	0.49
	Heart	33.09	21.60	11.85	0.27
	Kidney	29.93	21.97	8.32	3.13
	Lung	32.10	19.31	13.15	0.11
	Mammary Gland	30.00	21.74	8.61	2.56
	Pancreas	34.83	24.07	11.11	0.45
	Placenta	36.60	23.77	13.19	0.00
	Prostate	29.14	20.93	8.55	2.66
	Salivary Gland	32.11	21.39	11.07	0.46
	Skeletal Muscle	28.27	20.44	8.18	3.45
	Small Intestine	34.33	21.00	13.69	0.08
	Spinal Cord	27.04	21.91	5.47	22.48
	Spleen	32.45	19.02	13.78	0.07
	Stomach	32.15	21.66	10.84	0.55
	Testis	28.57	23.07	5.87	17.16
	Thymus	31.01	20.68	10.69	0.61
	Thyroid	28.84	20.80	8.39	2.97
	Trachea	31.39	19.63	12.11	0.23
	Uterus	30.37	21.09	9.64	1.25
	PBMC/Control	33.98	18.82	15.52	0.02
	PBMC/PMA	33.62	18.81	15.17	0.03
	PBMC/PHA	34.20	18.77	15.78	0.02
	PBMC/HDM	34.23	17.81	16.77	0.01
	A549 Cells	31.98	21.57	10.77	0.57
	THP-1	35.48	20.75	15.09	0.00
	Ovary	31.84	21.55	10.65	0.62
	(+ve) Positive Control	29.61	21.86	8.11	3.62

TABLE 5
Expression of hDUB 4.2, 4.3, 4.5 and 8.1 examined by primer 4.2
in human immunocytes panel:
Cell Type and stimulation		β2
condition	Mean	Mean	∂∂ Ct	Expression
Granulocyte resting	34.18	17.22	17.50	0.005
Granulocyte TNF-4/24 hr	32.39	17.16	15.76	0.018
CD19 (tonsillar - CD40L)	28.7	19.92	9.32	1.565
CD19 (tonsillar - LPS)	31.14	20.67	11.00	0.488
FLS-REST	34.67	20.43	14.78	0.036
FLS-IL1 4/24 hr	34.26	20.41	14.38	0.047
FLS-TNF-4/24 hr	34.91	20.15	15.31	0.025
Monocyte resting (pool 1.5, 7,	33.63	18.29	15.89	0.017
24 hr)
Monocyte LPS (pool 1.5, 7, 24 hr)	34.55	18.03	17.06	0.007
Monocyte INF-g (pool 1.5, 7,	34.62	17.27	17.88	0.004
24 hr)
Monocyte LPS & IFN-(pool	34.87	17.38	18.03	0.004
1.5, 7, 24 hr)
DCs progenitors (CD14+)	35.87	19.73	16.67	0.000
DCs immature	35.48	18.18	17.84	0.000
DCs mature	37.46	17.92	20.07	0.000
TH0 resting	31.11	17.63	14.02	0.060
TH0 activated	31.29	18.23	13.60	0.081
Th1 resting	33.88	18.27	16.15	0.014
Th1 CD28/CD3	32.15	19.31	13.38	0.094
Th2 resting	33.94	18.07	16.40	0.012
Th2 CD28/CD3	33.27	18.78	15.02	0.030
BSMC	35.33	21.64	14.22	0.000
BSMC IL-4 + TNF-24 hr	36.44	21.52	15.45	0.000
BSMC IL-13 + TNF-24 hr	35.94	21.41	15.07	0.000
BSMC IL-4 + IL-13	36.28	22.09	14.73	0.000
NHBE d0	36.63	22.24	14.92	0.000
NHBE IL-4 + TNF-d0	35.72	21.42	14.83	0.000
NHBE IL-13 + TNF-d0	36.35	21.37	15.52	0.000
NHBE resting d7 + d14	34.89	22.41	13.01	0.121
NHBE IL-4 + TNF-d7 + d14	38.59	22.02	17.11	0.000
NHBE IL-13 + TNF-d7 + d14	37.62	21.93	16.23	0.000
CD8 T cell O hour	30.15	19.52	11.16	0.437
CD8 T cell a-CD3/CD28 4 hour	32.08	19.6	13.01	0.121
CD8 T cell a-CD3/CD28 24	30.94	18.64	12.84	0.137
hour
HMVEC resting	35.09	20.25	15.38	0.000
HMVEC TNF-+ IL-4 24 hr	35.91	20.86	15.59	0.000
HMVEC TNF-24 hr	35.57	21.06	15.05	0.000
HMVEC TNF-+ IL-13 24 hr	36.38	20.61	16.31	0.000
Normal synovium pool	34.92	21.16	14.3	0.050
RA synovium pool	33.65	20.88	13.3	0.099
Normal colon	32.5	21.68	11.36	0.381
Colitis Colon	33.17	21.32	12.39	0.187
Crohns colon pooled	32.91	22.06	11.39	0.374
Normal Lung	31.01	20.5	11.05	0.472
COPD Lung	35.09	22.14	13.49	0.000
Positive control	28.4	22.29	6.64	9.992

Cloning of hDUB4, 8s by PCR

Following primer set was used to clone 530 amino acid open reading frame portion of single exon hDUB4s and 8s from human genomic DNA:

N-terminal primer:
5′-atggaggacgactcactct-3′ (19 mer, Seq ID No.1)
C-terminal primer:
5′-ctggcacacaagcaga-3′ (19 mer, Seq ID No.2)

Underlined triplet nucleotides in each primer represent translational initiation and termination codon. This primer set can amplify most of hDUB4s and hDUB8s as well as potentially yet to be identified hDUBs that are similar enough to hDUB4s and hDUB8s due to the high homology in nucleotide sequences in this part of the ORF. 1593 base pair fragment was successfully amplified from genomic DNA from two healthy human subjects and cloned into pCR2.1 vector and transformed into TOP10 strain of E. coli. Over 300 independent clones with appropriate size insert were obtained and sequences are obtained by ABI automated DNA sequencers.

Deubiqititination Assay

Confirmation that the DUB is a deubiquitinating enzyme may be shown using previously identified deubiquitination assay of ubiquitin—galactosidase fusion proteins, as described previously in the literature. Briefly, a fragment of the DUB, of approximately 1,500 nucleotides, based on the wild-type DUB cDNA (corresponding to amino acids 1 to about 500) and a cDNA containing a missense mutation are generated by PCR and inserted, in frame, into pGEX (Pharmacia), downstream of the glutathione S-transferase (GST) coding element. Ub-Met—gal is expressed from a pACYC184-based plasmid. Plasmids are co-transformed as indicated into MC 1061 Escherichia coli. Plasmid-bearing E. coli MC 1061 cells are lysed and analyzed by immunoblotting with a rabbit anti—gal antiserum (Cappel), arabbit anti-GST antiserum (Santa Cruz), and the ECL system (Amersham Corp.). in vitro deubiquitinating enzyme activity may be shown from purified hDUB fusion protein using commercial polyubiquitinated protein as substrate.

HDUB4s and hDUB8s are Potential Inflammatory Cytokines Specific Immediate-Early Genes

mDUB-1 was originally cloned as an IL-3-inducible immediate-early gene. Similarly, in DUB-2 was cloned as an IL-2-inducible immediate-early gene. We examined inducibility as well as cell-type specific expression of these genes using multiple TaqMan analysis from human organ RNA samples and human immunocytes RNA samples. Our data suggest that expression of hDUBs are not apparent in lymphocytes and granulocytes but high in fresh human PBMC from several donor. This strongly suggest that expression may be limited to the monocytes/macrophages and potentially NK cells. hDUB4s and hDUB8s are upregulated in PBMC stimulated with stimuli (LPS and/or PHA) that is known to upregulate various inflammatory cytokines such as TNF-alpha, IL-1 beta etc. This increase of expression is almost completely disappeared 20 to 24 hours after stimulation suggesting this is an early gene. The fact that there is only weak expression upregulation at 1.5 hours after stimulation suggests that stimuli by themselves may not upregulate hDUB4s and hDUB8s, but cytokines that are upregulated within couple of hours after stimulation may be responsible for upregulation of the hDUB4s and hDUB8s.

The DUB Subfamily of the ubp Superfamily

From these data we propose that hDUB4s and hDUB8s are members of a discrete subfamily of deubiquitinating enzymes that shows the strongest similarity to mDUB subfamily including mDUB1, mDUB2, and mDUB2A, called the DUB subfamily. DUB subfamily members contain distinct structural features that distinguish them from other ubps. First, DUB subfamily members are comparatively small enzymes of approximately 500-550 amino acids. Second, DUB subfamily members share amino acid similarity not only in the Cys and His domains but also throughout their primary amino acid sequence. For instance, DUB proteins contain a lysine-rich region (Lys domain) and a HV domain near their carboxyl terminus.

The regulatory regions, or promoter regions, of each of the DUBs was analyzed for putative transcription factor binding motifs using TRANSFACFind, a dynamic programming method, see Heinemeyer, T., et al., “Expanding the TRANSFAC database towards an expert system of regulatory molecular mechanisms” Nucleic Acids Res. 27, 318-322, (1999). The Transfac database provides eukaryotic cis- and trans-acting regulatory elements.

TABLE 6
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 4.1a. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00271	729 . . . 724(100)	AML-1a	runt-factor AML-1
M00148	296 . . . 302(100)	SRY	sex-determining region Y gene product
	1016 . . . 1010(96)
	958 . . . 964(94)
	474 . . . 480(94)
	1982 . . . 1988(92)
	129 . . . 123(90)
	857 . . . 863(90)
	776 . . . 782(90)
	1919 . . . 1913(90)
	1227 . . . 1233(90)
	276 . . . 282(90)
	1741 . . . 1735(90)
	193 . . . 199(90)
	105 . . . 111(90)
M00240	1600 . . . 1606(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
	700 . . . 694(100)
M00083	892 . . . 899(100)	MZF1	MZF1
M00101	162 . . . 156(100)	CdxA	CdxA
	1008 . . . 1002(100)
	423 . . . 429(100)
	153 . . . 147(99)
	359 . . . 353(98)
	1388 . . . 1394(98)
	1644 . . . 1650(97)
	1702 . . . 1696(97)
	250 . . . 256(97)
	231 . . . 237(97)
	617 . . . 611(94)
	509 . . . 503(93)
	432 . . . 426(92)
	307 . . . 313(92)
	153 . . . 159(92)
	1832 . . . 1838(92)
	1366 . . . 1372(92)
	494 . . . 500(92)
	1450 . . . 1456(91)
	1456 . . . 1450(91)
	722 . . . 716(90)
	991 . . . 985(90)
	986 . . . 992(90)
	1646 . . . 1640(90)
M00253	1142 . . . 1149(97)	cap	cap signal for transcription initiation
	1344 . . . 1351(96)
	639 . . . 632(95)
	1313 . . . 1320(94)
	1872 . . . 1879(93)
	269 . . . 262(92)
	257 . . . 250(91)
	1103 . . . 1110(91)
	745 . . . 752(91)
	1589 . . . 1596(90)
M00099	978 . . . 993(96)	S8	S8
	1637 . . . 1652(94)
	995 . . . 980(93)
M00100	162 . . . 156(96)	CdxA	CdxA
	1008 . . . 1002(96)
	423 . . . 429(96)
	1774 . . . 1768(96)
	415 . . . 421(92)
	860 . . . 854(91)
	1026 . . . 1020(91)
	494 . . . 500(91)
	94 . . . 100(91)
M00285	725 . . . 713(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	982 . . . 970(92)
M00347	531 . . . 522(95)	GATA-1	GATA-binding factor 1
M00135	1642 . . . 1660(95)	Oct-1	octamer factor 1
M00075	217 . . . 226(94)	GATA-1	GATA-binding factor 1
M00278	530 . . . 522(94)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins,
			and GATA-1, half-site 2
	900 . . . 908(90)
M00157	990 . . . 978(94)	RORalpha2	RAR-related orphan receptor alpha2
M00127	533 . . . 520(93)	GATA-1	GATA-binding factor 1
M00109	1933 . . . 1920(93)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00190	1656 . . . 1643(93)	C/EBP	CCAAT/enhancer binding factor
M00137	1193 . . . 1205(93)	Oct-1	octamer factor 1
	248 . . . 260(90)
	1652 . . . 1640(90)
M00302	1501 . . . 1512(92)	NF-AT	Nuclear factor of activated T-cells
M00077	900 . . . 908(91)	GATA-3	GATA-binding factor 3
	530 . . . 522(90)
M00126	533 . . . 520(91)	GATA-1	GATA-binding factor 1
M00159	1231 . . . 1219(91)	C/EBP	CCAAT/enhancer binding protein
M00074	1280 . . . 1292(91)	c-Ets-1(p54)	c-Ets-1(p54)
M00042	192 . . . 201(91)	Sox-5	Sox-5
M00241	1650 . . . 1643(91)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
M00116	138 . . . 125(91)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00138	1640 . . . 1662(91)	Oct-1	octamer factor 1
M00128	532 . . . 520(90)	GATA-1	GATA-binding factor 1
M00248	1645 . . . 1656(90)	Oct-1	octamer factor 1
M00289	1009 . . . 1021(90)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)

TABLE 7
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 4.1b. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00254	1831 . . . 1820(99)	CCAAT	cellular and viral CCAAT box
M00101	832 . . . 826(98)	CdxA	CdxA
	727 . . . 721(92)
	70 . . . 64(92)
	570 . . . 564(92)
	523 . . . 529(92)
	425 . . . 431(92)
	1682 . . . 1688(91)
	1409 . . . 1415(91)
	1415 . . . 1409(91)
	1688 . . . 1682(91)
M00054	470 . . . 461(97)	NF-kappaB	NF-kappaB
	643 . . . 634(95)
MM00148	946 . . . 940(96)	SRY	sex-determining region Y gene product
	1564 . . . 1570(92)
	1528 . . . 1534(92)
	1092 . . . 1098(92)
	1048 . . . 1054(90)
	708 . . . 714(90)
	655 . . . 661(90)
	1360 . . . 1354(90)
	1824 . . . 1818(90)
	396 . . . 390(90)
	749 . . . 743(90)
	1016 . . . 1010(90)
	302 . . . 308(90)
M00053	470 . . . 461(95)	c-Rel	c-Rel
	643 . . . 634(94)
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1111 . . . 1123(91)
	17 . . . 5(90)
M00052	470 . . . 461(95)	NF-kappaB	NF-kappaB (p65)
	643 . . . 634(94)
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00253	485 . . . 492(95)	cap	cap signal for transcription initiation
	1893 . . . 1886(95)
	749 . . . 756(94)
	834 . . . 841(93)
	1484 . . . 1477(92)
	511 . . . 504(92)
	1194 . . . 1201(91)
	163 . . . 170(91)
	321 . . . 328(91)
	340 . . . 347(91)
	1815 . . . 1808(90)
	563 . . . 570(90)
M00096	652 . . . 660(95)	Pbx-1	Pbx-1
M00194	472 . . . 459(95)	NF-kappaB	NF-kappaB
M00209	1818 . . . 1831(94)	NF-Y	NF-Y binding site
M00116	1238 . . . 1225(94)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00203	1699 . . . 1689(94)	GATA-X	GATA binding site
	1227 . . . 1217(90)
M00241	535 . . . 542(94)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
M00033	759 . . . 746(94)	p300	p300
	333 . . . 320(90)
M00127	1703 . . . 1690(93)	GATA-1	GATA-binding factor 1
M00158	323 . . . 310(93)	COUP-TF	COUP/HNF-4 heterodimer
M00075	1889 . . . 1898(93)	GATA-1	GATA-binding factor 1
	142 . . . 133(90)
	1736 . . . 1745(90)
M00286	963 . . . 976(93)	GKLF	gut-enriched Krueppel-like factor
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins,
			and GATA-1, half-site 2
M00076	1932 . . . 1941(93)	GATA-2	GATA-binding factor 2
	983 . . . 992(92)
M00208	471 . . . 460(93)	NF-kappaB	NF-kappaB binding site
M00185	1829 . . . 1819(92)	NF-Y	nuclear factor Y (Y-box binding factor)
M00302	232 . . . 243(92)	NF-AT	Nuclear factor of activated T-cells
M00348	98 . . . 107(92)	GATA-2	GATA-binding factor 2
M00134	308 . . . 326(92)	HNF-4	hepatic nuclear factor 4
M00223	548 . . . 540(92)	STATx	signal transducers and activators of transcription
M00039	1046 . . . 1039(92)	CREB	cAMP-responsive element binding protein
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00074	231 . . . 243(91)	c-Ets-1(p54)	c-Ets-1(p54)
	252 . . . 264(91)
M00289	385 . . . 397(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00199	1722 . . . 1714(91)	AP-1	AP-1 binding site
M00032	254 . . . 263(91)	c-Ets-1(p54)	c-Ets-1(p54)
M00147	782 . . . 773(91)	HSF2	heat shock factor 2
M00100	1101 . . . 1095(91)	CdxA	CdxA
M00042	650 . . . 659(90)	Sox-5	Sox-5
M00183	1026 . . . 1035(90)	c-Myb	c-Myb
M00240	963 . . . 957(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
	1272 . . . 1266(90)
M00190	1238 . . . 1225(90)	C/EBP	CCAAT/enhancer binding factor
M00083	49 . . . 42(90)	MZF1	MZF1
M00184	275 . . . 284(90)	MyoD	myoblast determining factor
M00087	980 . . . 991(90)	Ik-2	Ikaros 2
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00137	1388 . . . 1376(90)	Oct-1	octamer factor 1

TABLE 8
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 4.2a. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00148	992 . . . 986(100)	SRY	sex-determining region Y gene product
	942 . . . 948(100)
	919 . . . 913(96)
	1544 . . . 1550(92)
	1505 . . . 1511(92)
	815 . . . 809(90)
	1068 . . . 1074(90)
	1196 . . . 1190(90)
	1337 . . . 1331(90)
	680 . . . 686(90)
	1697 . . . 1691(90)
	1802 . . . 1796(90)
	368 . . . 362(90)
	721 . . . 715(90)
	274 . . . 280(90)
M00100	1077 . . . 1071(100)	CdxA	CdxA
M00271	1933 . . . 1938(100)	AML-1a	runt-factor AML-1
	2204 . . . 2209(92)
M00101	1077 . . . 1071(99)	CdxA	CdxA
	805 . . . 799(98)
	699 . . . 693(92)
	1384 . . . 1390(92)
	936 . . . 942(92)
	495 . . . 501(92)
	1660 . . . 1666(91)
	1666 . . . 1660(91)
M00076	716 . . . 707(98)	GATA-2	GATA-binding factor 2
	1910 . . . 1919(95)
	959 . . . 968(92)
	1679 . . . 1670(91)
M00285	1712 . . . 1724(96)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1087 . . . 1099(91)
M00272	1242 . . . 1251(96)	p53	tumor suppressor p53
M00253	135 . . . 142(96)	cap	cap signal for transcription initiation
	457 . . . 464(95)
	1871 . . . 1864(95)
	721 . . . 728(94)
	1461 . . . 1454(92)
	312 . . . 319(92)
	1989 . . . 1996(92)
	1855 . . . 1848(91)
	770 . . . 777(90)
	1793 . . . 1786(90)
	295 . . . 302(90)
	1274 . . . 1281(90)
M00106	627 . . . 636(95)	CDP	cut-like homeodomain protein
	634 . . . 625(93)
M00116	1215 . . . 1202(95)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00254	1809 . . . 1798(95)	CCAAT	cellular and viral CCAAT box
M00249	1117 . . . 1105(95)	CHOP-	heterodimers of CHOP and C/EBPalpha
		C/EBPalpha
M00054	442 . . . 433(95)	NF-kappaB	NF-kappaB
M00147	2182 . . . 2173(94)	HSF2	heat shock factor 2
	2173 . . . 2182(92)
	754 . . . 745(91)
	141 . . . 132(90)
M00104	634 . . . 625(94)	CDP	cut-like homeodomain protein
	2 . . . 11(92)
M00134	280 . . . 298(94)	HNF-4	hepatic nuclear factor 4
M00052	442 . . . 433(94)	NF-kappaB	NF-kappaB (p65)
M00053	442 . . . 433(94)	c-Rel	c-Rel
	785 . . . 794(90)
M00033	731 . . . 718(94)	p300	p300
M00158	295 . . . 282(93)	COUP-TF	COUP/HNF-4 heterodimer
M00032	225 . . . 216(93)	c-Ets-1(p54)	c-Ets-1(p54)
	226 . . . 235(93)
M00172	1851 . . . 1861(92)	AP-1	activator protein 1
M00223	520 . . . 512(92)	STATx	signal transducers and activators of transcription
M00075	1679 . . . 1670(92)	GATA-1	GATA-binding factor 1
	1867 . . . 1876(91)
	716 . . . 707(91)
	316 . . . 307(90)
M00184	1463 . . . 1472(91)	MyoD	myoblast determining factor
	1472 . . . 1463(91)
	247 . . . 256(90)
	2057 . . . 2048(90)
M00289	357 . . . 369(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00109	1202 . . . 1215(91)	C/EBPbeta	CCAAT/enhancer binding protein beta
	2036 . . . 2023(90)
M00268	937 . . . 950(91)	XFD-2	Xenopus fork head domain factor 2
M00208	443 . . . 432(90)	NF-kappaB	NF-kappaB binding site
M00173	1851 . . . 1861(90)	AP-1	activator protein 1
M00183	1002 . . . 1011(90)	c-Myb	c-Myb
	2020 . . . 2011(90)
M00240	217 . . . 211(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00188	1851 . . . 1861(90)	AP-1	activator protein 1
M00099	1086 . . . 1101(90)	S8	S8
M00302	813 . . . 802(90)	NF-AT	Nuclear factor of activated T-cells
M00083	21 . . . 14(90)	MZF1	MZF1
M00190	1215 . . . 1202(90)	C/EBP	CCAAT/enhancer binding factor
M00221	1838 . . . 1828(90)	SREBP-1	sterol regulatory element-binding protein 1
M00294	949 . . . 937(90)	HFH-8	HNF-3/Fkh Homolog-8
M00137	1365 . . . 1353(90)	Oct-1	octamer factor 1
M00077	1911 . . . 1919(90)	GATA-3	GATA-binding factor 3
M00194	444 . . . 431(90)	NF-kappaB	NF-kappaB

TABLE 9
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 4.2b. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00100	1102 . . . 1096(100)	CdxA	CdxA
M00148	1017 . . . 1011(100)	SRY	sex-determining region Y gene product
	967 . . . 973(100)
	944 . . . 938(96)
	1566 . . . 1572(92)
	1530 . . . 1536(92)
	840 . . . 834(90)
	1093 . . . 1099(90)
	705 . . . 711(90)
	1362 . . . 1356(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	393 . . . 387(90)
	746 . . . 740(90)
	299 . . . 305(90)
M00253	1120 . . . 1127(99)	cap	cap signal for transcription initiation
	160 . . . 167(96)
	482 . . . 489(95)
	1893 . . . 1886(95)
	746 . . . 753(94)
	1486 . . . 1479(92)
	337 . . . 344(92)
	1877 . . . 1870(91)
	795 . . . 802(90)
	1815 . . . 1808(90)
	320 . . . 327(90)
	1299 . . . 1306(90)
M00101	1102 . . . 1096(99)	CdxA	CdxA
	830 . . . 824(98)
	1231 . . . 1225(98)
	1409 . . . 1415(92)
	724 . . . 718(92)
	520 . . . 526(92)
	1682 . . . 1688(91)
	1688 . . . 1682(91)
M00076	741 . . . 732(98)	GATA-2	GATA-binding factor 2
	1932 . . . 1941(95)
	984 . . . 993(92)
	1701 . . . 1692(91)
M00285	1734 . . . 1746(96)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1112 . . . 1124(91)
M00272	1267 . . . 1276(96)	p53	tumor suppressor p53
M00106	652 . . . 661(95)	CDP	cut-like homeodomain protein
	659 . . . 650(93)
M00116	1240 . . . 1227(95)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00254	1831 . . . 1820(95)	CCAAT	cellular and viral CCAAT box
M00249	1142 . . . 1130(95)	CHOP-	heterodimers of CHOP and C/EBPalpha
		C/EBPalpha
M00054	467 . . . 458(95)	NF-kappaB	NF-kappaB
M00104	659 . . . 650(94)	CDP	cut-like homeodomain protein
	27 . . . 36(92)
M00134	305 . . . 323(94)	HNF-4	hepatic nuclear factor 4
M00052	467 . . . 458(94)	NF-kappaB	NF-kappaB (p65)
M00053	467 . . . 458(94)	c-Rel	c-Rel
M00033	756 . . . 743(94)	p300	p300
M00158	320 . . . 307(93)	COUP-TF	COUP/HNF-4 heterodimer
M00075	1889 . . . 1898(93)	GATA-1	GATA-binding factor 1
	1701 . . . 1692(92)
	741 . . . 732(91)
	341 . . . 332(90)
	641 . . . 632(90)
M00160	965 . . . 976(93)	SRY	sex-determining region Y gene product
M00032	250 . . . 241(93)	c-Ets-	c-Ets-1(p54)
		1(p54)
	251 . . . 260(93)
M00172	1873 . . . 1883(92)	AP-1	activator protein 1
M00223	545 . . . 537(92)	STATx	signal transducers and activators of transcription
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00184	1488 . . . 1497(91)	MyoD	myoblast determining factor
	1497 . . . 1488(91)
	272 . . . 281(90)
M00289	382 . . . 394(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00109	1227 . . . 1240(91)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00147	779 . . . 770(91)	HSF2	heat shock factor 2
	166 . . . 157(90)
M00208	468 . . . 457(90)	NF-kappaB	NF-kappaB binding site
M00183	1027 . . . 1036(90)	c-Myb	c-Myb
M00173	1873 . . . 1883(90)	AP-1	activator protein 1
M00240	242 . . . 236(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
M00188	1873 . . . 1883(90)	AP-1	activator protein 1
M00302	838 . . . 827(90)	NF-AT	Nuclear factor of activated T-cells
M00083	46 . . . 39(90)	MZF1	MZF1
M00190	1240 . . . 1227(90)	C/EBP	CCAAT/enhancer binding factor
M00096	1115 . . . 1123(90)	Pbx-1	Pbx-1
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00194	469 . . . 456(90)	NF-kappaB	NF-kappaB
M00077	1933 . . . 1941(90)	GATA-3	GATA-binding factor 3

TABLE 10
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 4.3. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00148	1015 . . . 1009(100)	SRY	sex-determining region Y gene product
	965 . . . 971(100)
	942 . . . 936(96)
	1566 . . . 1572(92)
	1528 . . . 1534(92)
	838 . . . 832(90)
	1091 . . . 1097(90)
	1219 . . . 1213(90)
	703 . . . 709(90)
	1360 . . . 1354(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	391 . . . 385(90)
	744 . . . 738(90)
	297 . . . 303(90)
M00100	1100 . . . 1094(100)	CdxA	CdxA
M00101	1100 . . . 1094(99)	CdxA	CdxA
	828 . . . 822(98)
	1407 . . . 1413(92)
	722 . . . 716(92)
	959 . . . 965(92)
	518 . . . 524(92)
	1682 . . . 1688(91)
	1688 . . . 1682(91)
M00076	739 . . . 730(98)	GATA-2	GATA-binding factor 2
	1932 . . . 1941(95)
	982 . . . 991(92)
	1701 . . . 1692(91)
M00285	1734 . . . 1746(96)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1110 . . . 1122(91)
M00272	1265 . . . 1274(96)	p53	tumor suppressor p53
M00253	158 . . . 165(96)	cap	cap signal for transcription initiation
	480 . . . 487(95)
	1893 . . . 1886(95)
	744 . . . 751(94)
	1484 . . . 1477(92)
	335 . . . 342(92)
	1877 . . . 1870(91)
	793 . . . 800(90)
	318 . . . 325(90)
	1297 . . . 1304(90)
M00106	650 . . . 659(95)	CDP	cut-like homeodomain protein
	657 . . . 648(93)
M00116	1238 . . . 1225(95)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00254	1831 . . . 1820(95)	CCAAT	cellular and viral CCAAT box
M00249	1140 . . . 1128(95)	CHOP-	heterodimers of CHOP and C/EBPalpha
		C/EBPalpha
M00054	465 . . . 456(95)	NF-kappaB	NF-kappaB
M00104	657 . . . 648(94)	CDP	cut-like homeodomain protein
	25 . . . 34(92)
M00134	303 . . . 321(94)	HNF-4	hepatic nuclear factor 4
M00052	465 . . . 456(94)	NF-kappaB	NF-kappaB (p65)
M00053	465 . . . 456(94)	c-Rel	c-Rel
M00033	754 . . . 741(94)	p300	p300
M00158	318 . . . 305(93)	COUP-TF	COUP/HNF-4 heterodimer
M00075	1889 . . . 1898(93)	GATA-1	GATA-binding factor 1
	1701 . . . 1692(92)
	739 . . . 730(91)
	339 . . . 330(90)
M00160	963 . . . 974(93)	SRY	sex-determining region Y gene product
M00032	248 . . . 239(93)	c-Ets-	c-Ets-1(p54)
	249 . . . 258(93)	1(p54)
M00172	1873 . . . 1883(92)	AP-1	activator protein 1
M00223	543 . . . 535(92)	STATx	signal transducers and activators of transcription
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00173	1873 . . . 1883(91)	AP-1	activator protein 1
M00184	1486 . . . 1495(91)	MyoD	myoblast detennining factor
	1495 . . . 1486(91)
	270 . . . 279(90)
M00289	380 . . . 392(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00109	1225 . . . 1238(91)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00147	777 . . . 768(91)	HSF2	heat shock factor 2
	164 . . . 155(90)
M00208	466 . . . 455(90)	NF-kappaB	NF-kappaB binding site
M00183	1025 . . . 1034(90)	c-Myb	c-Myb
M00240	240 . . . 234(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
M00099	1109 . . . 1124(90)	S8	S8
M00302	836 . . . 825(90)	NF-AT	Nuclear factor of activated T-cells
M00083	44 . . . 37(90)	MZF1	MZF1
M00190	1238 . . . 1225(90)	C/EBP	CCAAT/enhancer binding factor
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00174	1873 . . . 1883(90)	AP-1	activator protein 1
M00077	1933 . . . 1941(90)	GATA-3	GATA-binding factor 3
M00194	467 . . . 454(90)	NF-kappaB	NF-kappaB

TABLE 11
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 4.4. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00100	1101 . . . 1095(100)	CdxA	CdxA
M00148	1016 . . . 1010(100)	SRY	sex-determining region Y gene product
	966 . . . 972(100)
	944 . . . 938(96)
	1566 . . . 1572(92)
	1529 . . . 1535(92)
	840 . . . 834(90)
	1092 . . . 1098(90)
	705 . . . 711(90)
	1361 . . . 1355(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	393 . . . 387(90)
	746 . . . 740(90)
	299 . . . 305(90)
M00101	1101 . . . 1095(99)	CdxA	CdxA
	830 . . . 824(98)
	1230 . . . 1224(98)
	1408 . . . 1414(92)
	724 . . . 718(92)
	520 . . . 526(92)
	1682 . . . 1688(91)
	1688 . . . 1682(91)
M00076	741 . . . 732(98)	GATA-2	GATA-binding factor 2
	1932 . . . 1941(95)
	983 . . . 992(92)
	1701 . . . 1692(91)
M00350	876 . . . 867(96)	GATA-3	GATA-binding factor 3
M00285	1734 . . . 1746(96)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1111 . . . 1123(91)
M00272	1266 . . . 1275(96)	p53	tumor suppressor p53
M00253	160 . . . 167(96)	cap	cap signal for transcription initiation
	482 . . . 489(95)
	1893 . . . 1886(95)
	746 . . . 753(94)
	1485 . . . 1478(92)
	337 . . . 344(92)
	1877 . . . 1870(91)
	795 . . . 802(90)
	1815 . . . 1808(90)
	320 . . . 327(90)
	1298 . . . 1305(90)
M00106	652 . . . 661(95)	CDP	cut-like homeodomain protein
	659 . . . 650(93)
M00116	1239 . . . 1226(95)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00254	1831 . . . 1820(95)	CCAAT	cellular and viral CCAAT box
M00249	1141 . . . 1129(95)	CHOP-	heterodimers of CHOP and C/EBPalpha
		C/EBPalpha
M00349	876 . . . 867(95)	GATA-2	GATA-binding factor 2
M00054	467 . . . 458(95)	NF-kappaB	NF-kappaB
M00104	659 . . . 650(94)	CDP	cut-like homeodomain protein
	27 . . . 36(92)
M00134	305 . . . 323(94)	HNF-4	hepatic nuclear factor 4
M00052	467 . . . 458(94)	NF-kappaB	NF-kappaB (p65)
M00053	467 . . . 458(94)	c-Rel	c-Rel
M00033	756 . . . 743(94)	p300	p300
M00348	876 . . . 867(93)	GATA-2	GATA-binding factor 2
M00158	320 . . . 307(93)	COUP-TF	COUP/HNF-4 heterodimer
M00075	1889 . . . 1898(93)	GATA-1	GATA-binding factor 1
	1701 . . . 1692(92)
	741 . . . 732(91)
	341 . . . 332(90)
M00160	964 . . . 975(93)	SRY	sex-determining region Y gene product
M00347	876 . . . 867(93)	GATA-1	GATA-binding factor 1
M00032	250 . . . 241(93)	c-Ets-1(p54)	c-Ets-1(p54)
	251 . . . 260(93)
M00172	1873 . . . 1883(92)	AP-1	activator protein 1
M00223	545 . . . 537(92)	STATx	signal transducers and activators of transcription
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00184	1487 . . . 1496(91)	MyoD	myoblast determining factor
	1496 . . . 1487(91)
	272 . . . 281(90)
M00289	382 . . . 394(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00109	1226 . . . 1239(91)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00208	468 . . . 457(90)	NF-kappaB	NF-kappaB binding site
M00183	1026 . . . 1035(90)	c-Myb	c-Myb
M00173	1873 . . . 1883(90)	AP-1	activator protein 1
M00240	242 . . . 236(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
M00188	1873 . . . 1883(90)	AP-1	activator protein 1
M00099	1110 . . . 1125(90)	S8	S8
M00302	838 . . . 827(90)	NF-AT	Nuclear factor of activated T-cells
M00083	46 . . . 39(90)	MZF1	MZF1
M00190	1239 . . . 1226(90)	C/EBP	CCAAT/enhancer binding factor
M00147	166 . . . 157(90)	HSF2	heat shock factor 2
M00080	874 . . . 864(90)	Evi-1	ectopic viral integration site 1 encoded factor
M00082	874 . . . 864(90)	Evi-1	ectopic viral integration site 1 encoded factor
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00194	469 . . . 456(90)	NF-kappaB	NF-kappaB
M00077	1933 . . . 1941(90)	GATA-3	GATA-binding factor 3

TABLE 12
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 4.5. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00148	963 . . . 969(100)	SRY	sex-determining region Y gene product
	1013 . . . 1007(100)
	940 . . . 934(96)
	1526 . . . 1532(92)
	1089 . . . 1095(90)
	836 . . . 830(90)
	701 . . . 707(90)
	1358 . . . 1352(90)
	1720 . . . 1714(90)
	1825 . . . 1819(90)
	389 . . . 383(90)
	742 . . . 736(90)
	295 . . . 301(90)
M00100	1098 . . . 1092(100)	CdxA	CdxA
M00101	1098 . . . 1092(99)	CdxA	CdxA
	826 . . . 820(98)
	1405 . . . 1411(92)
	720 . . . 714(92)
	957 . . . 963(92)
	516 . . . 522(92)
	1683 . . . 1689(91)
	1689 . . . 1683(91)
M00076	737 . . . 728(98)	GATA-2	GATA-binding factor 2
	980 . . . 989(92)
	1702 . . . 1693(91)
	1299 . . . 1308(90)
M00285	1735 . . . 1747(96)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1108 . . . 1120(91)
M00272	1263 . . . 1272(96)	p53	tumor suppressor p53
M00253	156 . . . 163(96)	cap	cap signal for transcription initiation
	478 . . . 485(95)
	742 . . . 749(94)
	1482 . . . 1475(92)
	333 . . . 340(92)
	791 . . . 798(90)
	1816 . . . 1809(90)
	316 . . . 323(90)
M00106	648 . . . 657(95)	CDP	cut-like homeodomain protein
	655 . . . 646(93)
M00116	1236 . . . 1223(95)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00254	1832 . . . 1821(95)	CCAAT	cellular and viral CCAAT box
M00249	1138 . . . 1126(95)	CHOP-	heterodimers of CHOP and C/EBPalpha
		C/EBPalpha
M00054	463 . . . 454(95)	NF-kappaB	NF-kappaB
M00183	1493 . . . 1484(94)	c-Myb	c-Myb
	1023 . . . 1032(90)
M00104	655 . . . 646(94)	CDP	cut-like homeodomain protein
	23 . . . 32(92)
M00134	301 . . . 319(94)	HNF-4	hepatic nuclear factor 4
M00052	463 . . . 454(94)	NF-kappaB	NF-kappaB (p65)
M00053	463 . . . 454(94)	c-Rel	c-Rel
	806 . . . 815(90)
M00033	752 . . . 739(94)	p300	p300
M00158	316 . . . 303(93)	COUP-TF	COUP/HNF-4 heterodimer
M00032	246 . . . 237(93)	c-Ets-	c-Ets-1(p54)
	247 . . . 256(93)	1(p54)
M00278	1300 . . . 1308(92)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00223	541 . . . 533(92)	STATx	signal transducers and activators of transcription
M00075	1702 . . . 1693(92)	GATA-1	GATA-binding factor 1
	737 . . . 728(91)
	337 . . . 328(90)
M00077	1300 . . . 1308(91)	GATA-3	GATA-binding factor 3
M00289	378 . . . 390(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00109	1223 . . . 1236(91)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00268	958 . . . 971(91)	XFD-2	Xenopus fork head domain factor 2
M00147	775 . . . 766(91)	HSF2	heat shock factor 2
	162 . . . 153(90)
M00208	464 . . . 453(90)	NF-kappaB	NF-kappaB binding site
M00240	238 . . . 232(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00099	1107 . . . 1122(90)	S8	S8
M00302	834 . . . 823(90)	NF-AT	Nuclear factor of activated T-cells
M00083	42 . . . 35(90)	MZF1	MZF1
M00184	268 . . . 277(90)	MyoD	myoblast determining factor
M00190	1236 . . . 1223(90)	C/EBP	CCAAT/enhancer binding factor
M00221	1861 . . . 1851(90)	SREBP-1	sterol regulatory element-binding protein 1
M00294	970 . . . 958(90)	HFH-8	HNF-3/Fkh Homolog-8
M00137	1386 . . . 1374(90)	Oct-1	octamer factor 1
M00194	465 . . . 452(90)	NF-kappaB	NF-kappaB

TABLE 13
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 8.1. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00148	1015 . . . 1009(100)	SRY	sex-determining region Y gene product
	965 . . . 971(100)
	942 . . . 936(96)
	1544 . . . 1550(92)
	838 . . . 832(90)
	702 . . . 708(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	390 . . . 384(90)
	1384 . . . 1390(90)
	1356 . . . 1350(90)
	296 . . . 302(90)
	63 . . . 69(90)
M00100	1100 . . . 1094(100)	CdxA	CdxA
	724 . . . 730(96)
	1547 . . . 1541(91)
M00349	93 . . . 102(100)	GATA-2	GATA-binding factor 2
M00350	93 . . . 102(100)	GATA-3	GATA-binding factor 3
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00348	93 . . . 102(100)	GATA-2	GATA-binding factor 2
M00101	1100 . . . 1094(99)	CdxA	CdxA
	828 . . . 822(98)
	1229 . . . 1223(98)
	1203 . . . 1209(94)
	721 . . . 715(92)
	959 . . . 965(92)
	1386 . . . 1380(92)
	65 . . . 59(92)
	517 . . . 523(92)
	419 . . . 425(92)
	1682 . . . 1688(91)
	1688 . . . 1682(91)
M00203	95 . . . 105(98)	GATA-X	GATA binding site
M00347	93 . . . 102(97)	GATA-1	GATA-binding factor 1
M00075	1838 . . . 1847(97)	GATA-1	GATA-binding factor 1
	1701 . . . 1692(95)
	137 . . . 128(92)
	1483 . . . 1492(92)
	1889 . . . 1898(91)
	1736 . . . 1745(90)
M00158	317 . . . 304(96)	COUP-	COUP/HNF-4 heterodimer
		TF
M00253	158 . . . 165(96)	cap	cap signal for transcription initiation
	1794 . . . 1801(95)
	479 . . . 486(95)
	1297 . . . 1304(93)
	337 . . . 344(93)
	48 . . . 55(93)
	1484 . . . 1477(92)
	1324 . . . 1317(91)
	557 . . . 564(91)
	1893 . . . 1886(91)
	1877 . . . 1870(91)
	793 . . . 800(90)
	1356 . . . 1363(90)
	1815 . . . 1808(90)
	317 . . . 324(90)
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1110 . . . 1122(91)
	13 . . . 1(90)
M00134	302 . . . 320(95)	HNF-4	hepatic nuclear factor 4
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00096	1827 . . . 1819(95)	Pbx-1	Pbx-1
M00141	451 . . . 459(94)	Lyf-1	LyF-1
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00076	1483 . . . 1492(93)	GATA-2	GATA-binding factor 2
	1932 . . . 1941(93)
	1701 . . . 1692(92)
	982 . . . 991(92)
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00099	1813 . . . 1798(92)	S8	S8
	1109 . . . 1124(90)
M00294	1551 . . . 1539(92)	HFH-8	HNF-3/Fkh Homolog-8
M00223	542 . . . 534(92)	STATx	signal transducers and activators of transcription
M00073	36 . . . 46(92)	deltaEF1	deltaEF1
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
	132 . . . 127(92)
M00137	335 . . . 323(91)	Oct-1	octamer factor 1
M00042	644 . . . 653(91)	Sox-5	Sox-5
	1829 . . . 1820(90)
M00289	379 . . . 391(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00183	1025 . . . 1034(90)	c-Myb	c-Myb
M00240	239 . . . 233(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00302	836 . . . 825(90)	NF-AT	Nuclear factor of activated T-cells
M00128	92 . . . 104(90)	GATA-1	GATA-binding factor 1
M00184	269 . . . 278(90)	MyoD	myoblast determining factor
M00147	164 . . . 155(90)	HSF2	heat shock factor 2
M00087	979 . . . 990(90)	Ik-2	Ikaros 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1

TABLE 14
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.2. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00241	1804 . . . 1811(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00240	556 . . . 562(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
	1354 . . . 1360(90)
	301 . . . 307(90)
M00096	1342 . . . 1350(100)	Pbx-1	Pbx-1
	1831 . . . 1823(96)
M00271	702 . . . 707(100)	AML-1a	runt-factor AML-1
	1955 . . . 1960(92)
	170 . . . 175(92)
M00050	104 . . . 111(100)	E2F	E2F
M00148	1271 . . . 1277(100)	SRY	sex-determining region Y gene product
	1572 . . . 1578(92)
	1345 . . . 1351(90)
	1719 . . . 1713(90)
	1828 . . . 1822(90)
	1634 . . . 1628(90)
M00272	472 . . . 463(97)	p53	tumor suppressor p53
	463 . . . 472(97)
	295 . . . 286(91)
M00075	1842 . . . 1851(97)	GATA-1	GATA-binding factor 1
	1893 . . . 1902(93)
	1380 . . . 1371(92)
	1740 . . . 1749(90)
M00253	1072 . . . 1065(96)	cap	cap signal for transcription initiation
	1798 . . . 1805(95)
	1897 . . . 1890(95)
	769 . . . 776(92)
	1701 . . . 1708(92)
	1881 . . . 1874(91)
	497 . . . 504(90)
	1819 . . . 1812(90)
	21 . . . 28(90)
M00285	1738 . . . 1750(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	366 . . . 378(93)
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00147	148 . . . 139(94)	HSF2	heat shock factor 2
	139 . . . 148(92)
M00199	1801 . . . 1793(94)	AP-1	AP-1 binding site
	1037 . . . 1029(94)
	1793 . . . 1801(91)
	1029 . . . 1037(90)
M00174	1792 . . . 1802(94)	AP-1	activator protein 1
	1038 . . . 1028(92)
M00074	1247 . . . 1259(93)	c-Ets-	c-Ets-1(p54)
		1(p54)
M00211	702 . . . 710(93)	Poly	Retroviral Poly A downstream element
	1531 . . . 1539(91)
M00124	1339 . . . 1353(93)	Pbx1b	homeo domain factor Pbx-1
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00052	1261 . . . 1252(93)	NF-	NF-kappaB (p65)
		kappaB
M00076	1932 . . . 1941(93)	GATA-2	GATA-binding factor 2
	837 . . . 828(90)
M00099	1817 . . . 1802(92)	S8	S8
M00101	987 . . . 981(92)	CdxA	CdxA
M00042	1635 . . . 1626(92)	Sox-5	Sox-5
M00254	1835 . . . 1824(91)	CCAAT	cellular and viral CCAAT box
M00008	252 . . . 243(91)	Sp1	stimulating protein 1
	1323 . . . 1314(91)
M00227	933 . . . 941(90)	v-Myb	v-Myb
M00141	1328 . . . 1320(90)	Lyf-1	LyF-1
M00183	1033 . . . 1042(90)	c-Myb	c-Myb
M00001	654 . . . 665(90)	MyoD	myoblast determination gene product
M00188	1792 . . . 1802(90)	AP-1	activator protein 1
M00184	23 . . . 14(90)	MyoD	myoblast determining factor
M00172	1792 . . . 1802(90)	AP-1	activator protein 1
M00221	1864 . . . 1854(90)	SREBP-1	sterol regulatory element-binding protein 1
M00037	871 . . . 861(90)	NF-E2	NF-E2 p45
M00053	1261 . . . 1252(90)	c-Rel	c-Rel
M00249	823 . . . 835(90)	CHOP-	heterodimers of CHOP and C/EBPalpha
		C/EBPalpha
M00302	1248 . . . 1259(90)	NF-AT	Nuclear factor of activated T-cells

TABLE 15
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.3. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00348	71 . . . 80(100)	GATA-2	GATA-binding factor 2
M00349	71 . . . 80(100)	GATA-2	GATA-binding factor 2
M00148	990 . . . 984(100)	SRY	sex-determining region Y gene product
	1654 . . . 1648(100)
	813 . . . 807(96)
	917 . . . 911(96)
	1848 . . . 1854(92)
	1840 . . . 1846(92)
	1826 . . . 1832(92)
	1504 . . . 1498(92)
	1391 . . . 1397(90)
	680 . . . 686(90)
	2094 . . . 2088(90)
	368 . . . 362(90)
	721 . . . 715(90)
	1002 . . . 996(90)
	1900 . . . 1894(90)
	274 . . . 280(90)
M00350	71 . . . 80(100)	GATA-3	GATA-binding factor 3
M00100	595 . . . 589(100)	CdxA	CdxA
	1400 . . . 1394(100)
M00101	595 . . . 589(99)	CdxA	CdxA
	1400 . . . 1394(99)
	803 . . . 797(98)
	1527 . . . 1521(98)
	1705 . . . 1711(92)
	699 . . . 693(92)
	934 . . . 940(92)
	43 . . . 37(92)
	495 . . . 501(92)
	397 . . . 403(92)
M00141	1274 . . . 1266(98)	Lyf-1	LyF-1
M00347	71 . . . 80(97)	GATA-1	GATA-binding factor 1
M00272	1563 . . . 1572(96)	p53	tumor suppressor p53
M00253	1128 . . . 1135(96)	cap	cap signal for transcription initiation
	136 . . . 143(96)
	2064 . . . 2071(95)
	457 . . . 464(95)
	2163 . . . 2156(95)
	721 . . . 728(94)
	660 . . . 653(94)
	1782 . . . 1775(92)
	312 . . . 319(92)
	1622 . . . 1615(91)
	535 . . . 542(91)
	2085 . . . 2078(91)
	2147 . . . 2140(91)
	1350 . . . 1357(91)
	768 . . . 775(90)
	295 . . . 302(90)
M00285	2004 . . . 2016(95)	TCF11	TCF11/KCR-F1/Nrfl homodimers
	1410 . . . 1422(91)
M00254	2101 . . . 2090(95)	CCAAT	cellular and viral CCAAT box
M00130	1647 . . . 1658(95)	HFH-2	HNF-3/Fkh Homolog 2
M00054	442 . . . 433(95)	NF-kappaB	NF-kappaB
M00077	2203 . . . 2211(95)	GATA-3	GATA-binding factor 3
M00052	442 . . . 433(94)	NF-kappaB	NF-kappaB (p65)
M00203	73 . . . 83(94)	GATA-X	GATA binding site
M00199	2067 . . . 2059(94)	AP-1	AP-1 binding site
	2059 . . . 2067(91)
	1984 . . . 1992(90)
M00174	2058 . . . 2068(94)	AP-1	activator protein 1
M00075	895 . . . 886(94)	GATA-1	GATA-binding factor 1
	2159 . . . 2168(93)
	1082 . . . 1073(93)
	1971 . . . 1962(92)
	2006 . . . 2015(90)
M00053	442 . . . 433(94)	c-Rel	c-Rel
M00241	2070 . . . 2077(94)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00076	716 . . . 707(94)	GATA-2	GATA-binding factor 2
	1599 . . . 1608(92)
	957 . . . 966(92)
	895 . . . 886(91)
	1971 . . . 1962(91)
M00106	627 . . . 636(94)	CDP	cut-like homeodomain protein
M00033	731 . . . 718(94)	p300	p300
M00227	2139 . . . 2131(94)	v-Myb	v-Myb
M00158	295 . . . 282(93)	COUP-TF	COUP/HNF-4 heterodimer
M00162	2070 . . . 2083(93)	Oct-1	octamer-binding factor 1
M00134	280 . . . 298(93)	HNF-4	hepatic nuclear factor 4
M00032	226 . . . 235(93)	c-Ets-1(p54)	c-Ets-1(p54)
M00117	1080 . . . 1067(92)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00223	520 . . . 512(92)	STATx	signal transducers and activators of transcription
M00042	1901 . . . 1892(92)	Sox-5	Sox-5
	622 . . . 631(91)
M00073	13 . . . 23(92)	deltaEF1	deltaEF1
M00099	2083 . . . 2068(91)	S8	S8
	2066 . . . 2081(90)
	1409 . . . 1424(90)
M00289	1647 . . . 1659(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
	357 . . . 369(91)
M00147	752 . . . 743(91)	HSF2	heat shock factor 2
	142 . . . 133(90)
M00208	443 . . . 432(90)	NF-kappaB	NF-kappaB binding site
M00217	115 . . . 108(90)	USF	USF binding site
M00183	1325 . . . 1334(90)	c-Myb	c-Myb
M00240	217 . . . 211(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00188	2058 . . . 2068(90)	AP-1	activator protein 1
M00184	1955 . . . 1964(90)	MyoD	myoblast determining factor
	247 . . . 256(90)
M00087	954 . . . 965(90)	Ik-2	Ikaros 2
M00128	70 . . . 82(90)	GATA-1	GATA-binding factor 1
M00172	2058 . . . 2068(90)	AP-1	activator protein 1
M00145	1339 . . . 1354(90)	Brn-2	POU factor Brn-2
M00062	1217 . . . 1205(90)	IRF-1	interferon regulatory factor 1
M00194	444 . . . 431(90)	NF-kappaB	NF-kappaB

TABLE 16
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.4. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00100	1105 . . . 1099(100)	CdxA	CdxA
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00148	1020 . . . 1014(100)	SRY	sex-determining region Y gene product
	970 . . . 976(100)
	947 . . . 941(96)
	1550 . . . 1556(92)
	843 . . . 837(90)
	707 . . . 713(90)
	1362 . . . 1356(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	395 . . . 389(90)
	748 . . . 742(90)
	300 . . . 306(90)
M00101	1105 . . . 1099(99)	CdxA	CdxA
	833 . . . 827(98)
	1385 . . . 1379(98)
	1409 . . . 1415(92)
	964 . . . 970(92)
	726 . . . 720(92)
	424 . . . 430(92)
M00075	1838 . . . 1847(97)	GATA-1	GATA-binding factor 1
	1889 . . . 1898(93)
	925 . . . 916(93)
	1736 . . . 1745(90)
M00272	1267 . . . 1276(96)	p53	tumor suppressor p53
M00253	161 . . . 168(96)	cap	cap signal for transcription initiation
	1794 . . . 1801(95)
	484 . . . 491(95)
	1893 . . . 1886(95)
	1452 . . . 1459(94)
	798 . . . 805(94)
	748 . . . 755(94)
	957 . . . 964(94)
	1486 . . . 1479(92)
	338 . . . 345(92)
	1697 . . . 1704(92)
	562 . . . 569(91)
	1877 . . . 1870(91)
	1815 . . . 1808(90)
	321 . . . 328(90)
	1326 . . . 1319(90)
	1299 . . . 1306(90)
M00096	1827 . . . 1819(96)	Pbx-1	Pbx-1
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrfl homodimers
	1115 . . . 1127(91)
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00054	469 . . . 460(95)	NF-kappaB	NF-kappaB
M00141	456 . . . 464(94)	Lyf-1	LyF-1
M00134	306 . . . 324(94)	HNF-4	hepatic nuclear factor 4
M00052	469 . . . 460(94)	NF-kappaB	NF-kappaB (p65)
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00053	469 . . . 460(94)	c-Rel	c-Rel
M00033	758 . . . 745(94)	p300	p300
	808 . . . 795(92)
M00158	321 . . . 308(93)	COUP-TF	COUP/HNF-4 heterodimer
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00076	1932 . . . 1941(93)	GATA-2	GATA-binding factor 2
	1303 . . . 1312(92)
	743 . . . 734(92)
	987 . . . 996(92)
	925 . . . 916(90)
M00099	1813 . . . 1798(92)	S8	S8
	1114 . . . 1129(90)
M00104	28 . . . 37(92)	CDP	cut-like homeodomain protein
M00223	547 . . . 539(92)	STATx	signal transducers and activators of transcription
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00254	1831 . . . 1820(91)	CCAAT	cellular and viral CCAAT box
M00042	649 . . . 658(91)	Sox-5	Sox-5
M00289	384 . . . 396(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00302	1384 . . . 1395(91)	NF-AT	Nuclear factor of activated T-cells
	841 . . . 830(90)
M00155	367 . . . 382(91)	ARP-1	apolipoprotein AI regulatory protein 1
M00208	470 . . . 459(90)	NF-kappaB	NF-kappaB binding site
M00183	1030 . . . 1039(90)	c-Myb	c-Myb
M00240	243 . . . 237(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
	1454 . . . 1448(90)
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00083	47 . . . 40(90)	MZF1	MZF1
M00184	273 . . . 282(90)	MyoD	myoblast determining factor
M00147	167 . . . 158(90)	HSF2	heat shock factor 2
M00087	984 . . . 995(90)	Ik-2	Ikaros 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00194	471 . . . 458(90)	NF-kappaB	NF-kappaB

TABLE 17
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.5. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00100	1105 . . . 1099(100)	CdxA	CdxA
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00271	27 . . . 32(100)	AML-1a	runt-factor AML-1
	1955 . . . 1960(92)
M00148	1020 . . . 1014(100)	SRY	sex-determining region Y gene product
	970 . . . 976(100)
	947 . . . 941(96)
	1553 . . . 1559(92)
	100 . . . 106(92)
	141 . . . 135(90)
	843 . . . 837(90)
	1365 . . . 1359(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
M00101	1105 . . . 1099(99)	CdxA	CdxA
	833 . . . 827(98)
	1234 . . . 1228(98)
	1388 . . . 1382(98)
	1300 . . . 1294(93)
	1412 . . . 1418(92)
	964 . . . 970(92)
	1682 . . . 1688(91)
	1688 . . . 1682(91)
M00278	618 . . . 610(98)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
	1933 . . . 1941(93)
M00075	1838 . . . 1847(97)	GATA-1	GATA-binding factor 1
	619 . . . 610(97)
	1701 . . . 1692(95)
	1889 . . . 1898(93)
	903 . . . 894(91)
	1736 . . . 1745(90)
M00054	442 . . . 451(96)	NF-	NF-kappaB
	451 . . . 442(91)	kappaB
M00253	175 . . . 168(96)	cap	cap signal for transcription initiation
	1794 . . . 1801(95)
	1893 . . . 1886(95)
	1455 . . . 1462(94)
	798 . . . 805(94)
	629 . . . 636(94)
	1489 . . . 1482(92)
	433 . . . 426(92)
	1329 . . . 1322(91)
	1877 . . . 1870(91)
	921 . . . 928(91)
	957 . . . 964(90)
	1815 . . . 1808(90)
	1302 . . . 1309(90)
M00272	1270 . . . 1279(96)	p53	tumor suppressor p53
M00096	1827 . . . 1819(96)	Pbx-1	Pbx-1
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1115 . . . 1127(91)
	1490 . . . 1478(91)
M00076	619 . . . 610(95)	GATA-2	GATA-binding factor 2
	1932 . . . 1941(93)
	1701 . . . 1692(92)
	1306 . . . 1315(92)
	987 . . . 996(92)
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
	618 . . . 610(92)
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00083	566 . . . 559(93)	MZF1	MZF1
M00099	1813 . . . 1798(92)	S8	S8
	1114 . . . 1129(90)
M00208	441 . . . 452(92)	NF-	NF-kappaB binding site
		kappaB
M00033	808 . . . 795(92)	p300	p300
M00227	1677 . . . 1669(91)	v-Myb	v-Myb
M00254	1831 . . . 1820(91)	CCAAT	cellular and viral CCAAT box
M00183	166 . . . 157(91)	c-Myb	c-Myb
	1030 . . . 1039(90)
M00267	102 . . . 89(91)	XFD-1	Xenopus fork head domain factor 1
M00240	1457 . . . 1451(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
	296 . . . 302(90)
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00302	841 . . . 830(90)	NF-AT	Nuclear factor of activated T-cells
M00087	984 . . . 995(90)	Ik-2	Ikaros 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00003	965 . . . 956(90)	v-Myb	v-Myb
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00053	441 . . . 450(90)	c-Rel	c-Rel

TABLE 18
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.6. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00100	1105 . . . 1099(100)	CdxA	CdxA
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
M00148	1020 . . . 1014(100)	SRY	sex-determining region Y gene product
	970 . . . 976(100)
	947 . . . 941(96)
	1550 . . . 1556(92)
	843 . . . 837(90)
	707 . . . 713(90)
	1362 . . . 1356(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	395 . . . 389(90)
	748 . . . 742(90)
	300 . . . 306(90)
M00101	1105 . . . 1099(99)	CdxA	CdxA
	833 . . . 827(98)
	1385 . . . 1379(98)
	1409 . . . 1415(92)
	964 . . . 970(92)
	726 . . . 720(92)
	424 . . . 430(92)
M00075	1838 . . . 1847(97)	GATA-1	GATA-binding factor 1
	1889 . . . 1898(93)
	925 . . . 916(93)
	1736 . . . 1745(90)
M00272	1267 . . . 1276(96)	p53	tumor suppressor p53
M00253	161 . . . 168(96)	cap	cap signal for transcription initiation
	1794 . . . 1801(95)
	484 . . . 491(95)
	1893 . . . 1886(95)
	1452 . . . 1459(94)
	798 . . . 805(94)
	748 . . . 755(94)
	957 . . . 964(94)
	1486 . . . 1479(92)
	338 . . . 345(92)
	1697 . . . 1704(92)
	562 . . . 569(91)
	1877 . . . 1870(91)
	1815 . . . 1808(90)
	321 . . . 328(90)
	1326 . . . 1319(90)
	1299 . . . 1306(90)
M00096	1827 . . . 1819(96)	Pbx-1	Pbx-1
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1115 . . . 1127(91)
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00054	469 . . . 460(95)	NF-kappaB	NF-kappaB
M00141	456 . . . 464(94)	Lyf-1	LyF-1
M00134	306 . . . 324(94)	HNF-4	hepatic nuclear factor 4
M00052	469 . . . 460(94)	NF-kappaB	NF-kappaB (p65)
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00053	469 . . . 460(94)	c-Rel	c-Rel
M00033	758 . . . 745(94)	p300	p300
	808 . . . 795(92)
M00158	321 . . . 308(93)	COUP-TF	COUP/HNF-4 heterodimer
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins,
			and GATA-1, half-site 2
M00076	1932 . . . 1941(93)	GATA-2	GATA-binding factor 2
	1303 . . . 1312(92)
	743 . . . 734(92)
	987 . . . 996(92)
	925 . . . 916(90)
M00099	1813 . . . 1798(92)	S8	S8
	1114 . . . 1129(90)
M00104	28 . . . 37(92)	CDP	cut-like homeodomain protein
M00223	547 . . . 539(92)	STATx	signal transducers and activators of transcription
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00254	1831 . . . 1820(91)	CCAAT	cellular and viral CCAAT box
M00042	649 . . . 658(91)	Sox-5	Sox-5
M00289	384 . . . 396(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00302	1384 . . . 1395(91)	NF-AT	Nuclear factor of activated T-cells
	841 . . . 830(90)
M00155	367 . . . 382(91)	ARP-1	apolipoprotein AI regulatory protein 1
M00208	470 . . . 459(90)	NF-kappaB	NF-kappaB binding site
M00183	1030 . . . 1039(90)	c-Myb	c-Myb
M00240	243 . . . 237(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman
			homolog
	1454 . . . 1448(90)
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00083	47 . . . 40(90)	MZF1	MZF1
M00184	273 . . . 282(90)	MyoD	myoblast determining factor
M00147	167 . . . 158(90)	HSF2	heat shock factor 2
M00087	984 . . . 995(90)	Ik-2	Ikaros 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00194	471 . . . 458(90)	NF-kappaB	NF-kappaB

TABLE 19
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.7. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00349	94 . . . 103(100)	GATA-2	GATA-binding factor 2
M00348	94 . . . 103(100)	GATA-2	GATA-binding factor 2
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00100	1102 . . . 1096(100)	CdxA	CdxA
M00148	1017 . . . 1011(100)	SRY	sex-determining region Y gene product
	967 . . . 973(100)
	704 . . . 710(100)
	944 . . . 938(96)
	1550 . . . 1556(92)
	840 . . . 834(90)
	1362 . . . 1356(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	392 . . . 386(90)
	745 . . . 739(90)
	298 . . . 304(90)
M00350	94 . . . 103(100)	GATA-3	GATA-binding factor 3
M00101	1102 . . . 1096(99)	CdxA	CdxA
	830 . . . 824(98)
	1231 . . . 1225(98)
	1385 . . . 1379(98)
	1297 . . . 1291(93)
	1409 . . . 1415(92)
	723 . . . 717(92)
	961 . . . 967(92)
	66 . . . 60(92)
	421 . . . 427(92)
	1682 . . . 1688(91)
	1688 . . . 1682(91)
M00203	96 . . . 106(98)	GATA-X	GATA binding site
M00347	94 . . . 103(97)	GATA-1	GATA-binding factor 1
M00075	1838 . . . 1847(97)	GATA-1	GATA-binding factor 1
	1701 . . . 1692(95)
	1889 . . . 1898(93)
	900 . . . 891(91)
	138 . . . 129(90)
	1736 . . . 1745(90)
M00158	319 . . . 306(96)	COUP-TF	COUP/HNF-4 heterodimer
M00272	1267 . . . 1276(96)	p53	tumor suppressor p53
M00134	304 . . . 322(96)	HNF-4	hepatic nuclear factor 4
M00096	1827 . . . 1819(96)	Pbx-1	Pbx-1
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1112 . . . 1124(91)
	1487 . . . 1475(91)
	13 . . . 1(90)
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00253	1794 . . . 1801(95)	cap	cap signal for transcription initiation
	481 . . . 488(95)
	1893 . . . 1886(95)
	1452 . . . 1459(94)
	795 . . . 802(94)
	656 . . . 649(93)
	1486 . . . 1479(92)
	1326 . . . 1319(91)
	559 . . . 566(91)
	1877 . . . 1870(91)
	745 . . . 752(91)
	918 . . . 925(91)
	954 . . . 961(90)
	1815 . . . 1808(90)
	1299 . . . 1306(90)
M00054	466 . . . 457(95)	NF-kappaB	NF-kappaB
M00146	165 . . . 156(94)	HSF1	heat shock factor 1
M00147	165 . . . 156(94)	HSF2	heat shock factor 2
	156 . . . 165(94)
M00141	453 . . . 461(94)	Lyf-1	LyF-1
M00052	466 . . . 457(94)	NF-kappaB	NF-kappaB (p65)
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00053	466 . . . 457(94)	c-Rel	c-Rel
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00076	1932 . . . 1941(93)	GATA-2	GATA-binding factor 2
	1701 . . . 1692(92)
	1303 . . . 1312(92)
	984 . . . 993(92)
M00099	1813 . . . 1798(92)	S8	S8
	1111 . . . 1126(90)
M00184	271 . . . 280(92)	MyoD	myoblast determining factor
M00223	544 . . . 536(92)	STATx	signal transducers and activators of transcription
M00073	36 . . . 46(92)	deltaEF1	deltaEF1
M00033	805 . . . 792(92)	p300	p300
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00254	1831 . . . 1820(91)	CCAAT	cellular and viral CCAAT box
M00277	281 . . . 270(91)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 1
M00289	381 . . . 393(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00208	467 . . . 456(90)	NF-kappaB	NF-kappaB binding site
M00183	1027 . . . 1036(90)	c-Myb	c-Myb
M00240	241 . . . 235(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
	1454 . . . 1448(90)
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00302	838 . . . 827(90)	NF-AT	Nuclear factor of activated T-cells
M00128	93 . . . 105(90)	GATA-1	GATA-binding factor 1
M00087	981 . . . 992(90)	Ik-2	Ikaros 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00042	69 . . . 78(90)	Sox-5	Sox-5
M00003	962 . . . 953(90)	v-Myb	v-Myb
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00194	468 . . . 455(90)	NF-kappaB	NF-kappaB

TABLE 20
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.8. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00148	1020 . . . 1014(100)	SRY	sex-determining region Y gene product
	970 . . . 976(100)
	947 . . . 941(96)
	1550 . . . 1556(92)
	843 . . . 837(90)
	707 . . . 713(90)
	1362 . . . 1356(90)
	1719 . . . 1713(90)
	1824 . . . 1818(90)
	395 . . . 389(90)
	748 . . . 742(90)
	300 . . . 306(90)
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00100	1105 . . . 1099(100)	CdxA	CdxA
M00101	1105 . . . 1099(99)	CdxA	CdxA
	833 . . . 827(98)
	1385 . . . 1379(98)
	1409 . . . 1415(92)
	964 . . . 970(92)
	726 . . . 720(92)
	424 . . . 430(92)
M00075	1838 . . . 1847(97)	GATA-1	GATA-binding factor 1
	1889 . . . 1898(93)
	925 . . . 916(93)
	1736 . . . 1745(90)
M00272	1267 . . . 1276(96)	p53	tumor suppressor p53
M00253	161 . . . 168(96)	cap	cap signal for transcription initiation
	1794 . . . 1801(95)
	484 . . . 491(95)
	1893 . . . 1886(95)
	1452 . . . 1459(94)
	798 . . . 805(94)
	748 . . . 755(94)
	957 . . . 964(94)
	1486 . . . 1479(92)
	338 . . . 345(92)
	1697 . . . 1704(92)
	562 . . . 569(91)
	1877 . . . 1870(91)
	1815 . . . 1808(90)
	321 . . . 328(90)
	1326 . . . 1319(90)
	1299 . . . 1306(90)
M00096	1827 . . . 1819(96)	Pbx-1	Pbx-1
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1115 . . . 1127(91)
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00054	469 . . . 460(95)	NF-kappaB	NF-kappaB
M00141	456 . . . 464(94)	Lyf-1	LyF-1
M00134	306 . . . 324(94)	HNF-4	hepatic nuclear factor 4
M00052	469 . . . 460(94)	NF-kappaB	NF-kappaB (p65)
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00053	469 . . . 460(94)	c-Rel	c-Rel
M00033	758 . . . 745(94)	p300	p300
	808 . . . 795(92)
M00158	321 . . . 308(93)	COUP-TF	COUP/HNF-4 heterodimer
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00076	1932 . . . 1941(93)	GATA-2	GATA-binding factor 2
	1303 . . . 1312(92)
	743 . . . 734(92)
	925 . . . 916(90)
M00099	1813 . . . 1798(92)	S8	S8
	1114 . . . 1129(90)
M00104	28 . . . 37(92)	CDP	cut-like homeodomain protein
M00223	547 . . . 539(92)	STATx	signal transducers and activators of transcription
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00254	1831 . . . 1820(91)	CCAAT	cellular and viral CCAAT box
M00042	649 . . . 658(91)	Sox-5	Sox-5
M00289	384 . . . 396(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00302	1384 . . . 1395(91)	NF-AT	Nuclear factor of activated T-cells
	841 . . . 830(90)
M00155	367 . . . 382(91)	ARP-1	apolipoprotein AI regulatory protein 1
M00208	470 . . . 459(90)	NF-kappaB	NF-kappaB binding site
M00183	1030 . . . 1039(90)	c-Myb	c-Myb
M00240	243 . . . 237(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
	1454 . . . 1448(90)
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00083	47 . . . 40(90)	MZF1	MZF1
M00184	273 . . . 282(90)	MyoD	myoblast determining factor
M00147	167 . . . 158(90)	HSF2	heat shock factor 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00194	471 . . . 458(90)	NF-kappaB	NF-kappaB

TABLE 21
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.9. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00148	991 . . . 997(100)	SRY	sex-determining region Y gene product
	1040 . . . 1034(100)
	967 . . . 961(96)
	1576 . . . 1582(92)
	1550 . . . 1556(92)
	1385 . . . 1379(90)
	1824 . . . 1818(90)
	13 . . . 19(90)
	415 . . . 409(90)
	727 . . . 733(90)
M00101	1125 . . . 1119(100)	CdxA	CdxA
	1254 . . . 1248(98)
	1167 . . . 1161(97)
	542 . . . 548(97)
	884 . . . 878(94)
	1432 . . . 1438(93)
	743 . . . 737(92)
	89 . . . 83(92)
	592 . . . 586(92)
	1280 . . . 1286(92)
	444 . . . 450(92)
	1682 . . . 1688(91)
	1688 . . . 1682(91)
M00083	779 . . . 772(100)	MZF1	MZF1
	68 . . . 61(90)
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00272	1290 . . . 1299(96)	p53	tumor suppressor p53
M00253	182 . . . 189(96)	cap	cap signal for transcription initiation
	1794 . . . 1801(95)
	1893 . . . 1886(95)
	1475 . . . 1482(94)
	768 . . . 775(94)
	1506 . . . 1499(93)
	1349 . . . 1342(91)
	582 . . . 589(91)
	351 . . . 358(90)
	1815 . . . 1808(90)
	342 . . . 349(90)
	1877 . . . 1870(90)
	504 . . . 511(90)
	1322 . . . 1329(90)
M00100	1125 . . . 1119(96)	CdxA	CdxA
M00042	742 . . . 751(96)	Sox-5	Sox-5
	669 . . . 678(92)
	726 . . . 735(92)
M00096	1827 . . . 1819(96)	Pbx-1	Pbx-1
M00077	1933 . . . 1941(95)	GATA-3	GATA-binding factor 3
M00045	1877 . . . 1888(95)	E4BP4	E4BP4
M00054	489 . . . 480(95)	NF-kappaB	NF-kappaB
M00141	476 . . . 484(94)	Lyf-1	LyF-1
M00052	489 . . . 480(94)	NF-kappaB	NF-kappaB (p65)
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00053	489 . . . 480(94)	c-Rel	c-Rel
M00278	1933 . . . 1941(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00076	1932 . . . 1941(93)	GATA-2	GATA-binding factor 2
	1007 . . . 1016(92)
	1701 . . . 1692(91)
M00285	1734 . . . 1746(92)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1135 . . . 1147(91)
M00099	1813 . . . 1798(92)	S8	S8
	1134 . . . 1149(90)
M00104	49 . . . 58(92)	CDP	cut-like homeodomain protein
M00223	567 . . . 559(92)	STATx	signal transducers and activators of transcription
M00075	1701 . . . 1692(92)	GATA-1	GATA-binding factor 1
	161 . . . 152(90)
	1736 . . . 1745(90)
M00348	316 . . . 325(92)	GATA-2	GATA-binding factor 2
M00109	873 . . . 886(91)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00254	1831 . . . 1820(91)	CCAAT	cellular and viral CCAAT box
M00203	318 . . . 328(91)	GATA-X	GATA binding site
M00350	316 . . . 325(91)	GATA-3	GATA-binding factor 3
M00289	404 . . . 416(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00113	833 . . . 822(90)	CREB	cAMP-responsive element binding protein
M00249	1165 . . . 1153(90)	CHOP-	heterodimers of CHOP and C/EBPalpha
		C/EBPalpha
M00208	490 . . . 479(90)	NF-kappaB	NF-kappaB binding site
M00190	1263 . . . 1250(90)	C/EBP	CCAAT/enhancer binding factor
M00349	316 . . . 325(90)	GATA-2	GATA-binding factor 2
M00116	1263 . . . 1250(90)	C/EBPalpha	CCAAT/enhancer binding protein alpha
M00183	1050 . . . 1059(90)	c-Myb	c-Myb
M00240	263 . . . 257(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
	1477 . . . 1471(90)
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00302	1407 . . . 1418(90)	NF-AT	Nuclear factor of activated T-cells
M00184	293 . . . 302(90)	MyoD	myoblast determining factor
M00147	188 . . . 179(90)	HSF2	heat shock factor 2
M00087	1004 . . . 1015(90)	Ik-2	Ikaros 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1
M00033	778 . . . 765(90)	p300	p300
M00194	491 . . . 478(90)	NF-kappaB	NF-kappaB

TABLE 22
putative transcription factor binding motifs within the DUB regulatory or
promoter, region of hDUB 8.10. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00148	1038 . . . 1032(100)	SRY	sex-determining region Y gene product
	965 . . . 959(96)
	1550 . . . 1556(92)
	725 . . . 731(90)
	1382 . . . 1376(90)
	310 . . . 316(90)
	1715 . . . 1709(90)
	1824 . . . 1818(90)
	12 . . . 18(90)
	413 . . . 407(90)
	1630 . . . 1624(90)
	319 . . . 325(90)
M00241	1800 . . . 1807(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00100	1123 . . . 1117(100)	CdxA	CdxA
M00240	1240 . . . 1234(100)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
	262 . . . 256(90)
M00141	474 . . . 482(100)	Lyf-1	LyF-1
M00101	1123 . . . 1117(99)	CdxA	CdxA
	540 . . . 546(97)
	1429 . . . 1435(94)
	982 . . . 988(92)
	88 . . . 82(92)
	741 . . . 735(92)
	442 . . . 448(92)
M00075	1838 . . . 1847(97)	GATA-1	GATA-binding factor 1
	1889 . . . 1898(93)
	1736 . . . 1745(90)
M00253	766 . . . 773(96)	cap	cap signal for transcription initiation
	181 . . . 188(96)
	1794 . . . 1801(95)
	1893 . . . 1886(95)
	1210 . . . 1217(94)
	816 . . . 823(94)
	1506 . . . 1499(92)
	357 . . . 364(92)
	1697 . . . 1704(92)
	1090 . . . 1097(91)
	1346 . . . 1339(91)
	1877 . . . 1870(91)
	71 . . . 78(90)
	580 . . . 587(90)
	1815 . . . 1808(90)
	340 . . . 347(90)
	502 . . . 509(90)
	1319 . . . 1326(90)
M00096	1827 . . . 1819(96)	Pbx-1	Pbx-1
M00285	1734 . . . 1746(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1133 . . . 1145(91)
	35 . . . 23(91)
	1089 . . . 1101(90)
M00134	325 . . . 343(94)	HNF-4	hepatic nuclear factor 4
M00199	1797 . . . 1789(94)	AP-1	AP-1 binding site
	1789 . . . 1797(91)
M00174	1788 . . . 1798(94)	AP-1	activator protein 1
M00042	740 . . . 749(94)	Sox-5	Sox-5
	1631 . . . 1622(92)
	667 . . . 676(91)
M00106	672 . . . 681(94)	CDP	cut-like homeodomain protein
M00158	340 . . . 327(93)	COUP-TF	COUP/HNF-4 heterodimer
M00076	761 . . . 752(93)	GATA-2	GATA-binding factor 2
	1323 . . . 1332(92)
	1005 . . . 1014(92)
M00099	1813 . . . 1798(92)	S8	S8
	1132 . . . 1147(90)
M00104	48 . . . 57(92)	CDP	cut-like homeodomain protein
M00223	565 . . . 557(92)	STATx	signal transducers and activators of transcription
M00033	826 . . . 813(92)	p300	p300
M00271	1955 . . . 1960(92)	AML-1a	runt-factor AML-1
M00254	1831 . . . 1820(91)	CCAAT	cellular and viral CCAAT box
M00289	402 . . . 414(91)	HFH-3	HNF-3/Fkh Homolog 3 (=Freac-6)
M00217	160 . . . 153(90)	USF	USF binding site
M00183	1048 . . . 1057(90)	c-Myb	c-Myb
M00188	1788 . . . 1798(90)	AP-1	activator protein 1
M00083	67 . . . 60(90)	MZF1	MZF1
M00184	292 . . . 301(90)	MyoD	myoblast determining factor
M00147	187 . . . 178(90)	HSF2	heat shock factor 2
M00087	1002 . . . 1013(90)	Ik-2	Ikaros 2
M00172	1788 . . . 1798(90)	AP-1	activator protein 1
M00221	1860 . . . 1850(90)	SREBP-1	sterol regulatory element-binding protein 1

TABLE 23
putative transcription factor binding motifs within the DUB regulatory or promoter,
region of hDUB 8.11. The position is indicated by nucleotides.
Transfac	Position(Score)	Name	Description
M00348	71 . . . 80(100)	GATA-2	GATA-binding factor 2
M00350	71 . . . 80(100)	GATA-3	GATA-binding factor 3
M00349	71 . . . 80(100)	GATA-2	GATA-binding factor 2
M00148	990 . . . 984(100)	SRY	sex-determining region Y gene product
	1664 . . . 1658(100)
	813 . . . 807(96)
	917 . . . 911(96)
	1858 . . . 1864(92)
	1850 . . . 1856(92)
	1836 . . . 1842(92)
	1399 . . . 1405(90)
	1514 . . . 1508(90)
	680 . . . 686(90)
	2104 . . . 2098(90)
	368 . . . 362(90)
	721 . . . 715(90)
	1002 . . . 996(90)
	1910 . . . 1904(90)
	274 . . . 280(90)
M00100	595 . . . 589(100)	CdxA	CdxA
	1408 . . . 1402(100)
	1474 . . . 1480(96)
M00101	595 . . . 589(99)	CdxA	CdxA
	1408 . . . 1402(99)
	803 . . . 797(98)
	1537 . . . 1531(98)
	1715 . . . 1721(92)
	699 . . . 693(92)
	934 . . . 940(92)
	43 . . . 37(92)
	495 . . . 501(92)
M00141	1282 . . . 1274(98)	Lyf-1	LyF-1
M00347	71 . . . 80(97)	GATA-1	GATA-binding factor 1
M00272	1573 . . . 1582(96)	p53	tumor suppressor p53
M00253	1137 . . . 1144(96)	cap	cap signal for transcription initiation
	136 . . . 143(96)
	2074 . . . 2081(95)
	457 . . . 464(95)
	2173 . . . 2166(95)
	721 . . . 728(94)
	1444 . . . 1437(94)
	660 . . . 653(94)
	1792 . . . 1785(92)
	312 . . . 319(92)
	1632 . . . 1625(91)
	535 . . . 542(91)
	2095 . . . 2088(91)
	1358 . . . 1365(91)
	768 . . . 775(90)
	295 . . . 302(90)
	2157 . . . 2150(90)
M00096	2107 . . . 2099(96)	Pbx-1	Pbx-1
M00285	2014 . . . 2026(95)	TCF11	TCF11/KCR-F1/Nrf1 homodimers
	1418 . . . 1430(91)
M00130	1657 . . . 1668(95)	HFH-2	HNF-3/Fkh Homolog 2
M00077	2213 . . . 2221(95)	GATA-3	GATA-binding factor 3
M00054	442 . . . 433(95)	NF-kappaB	NF-kappaB
M00052	442 . . . 433(94)	NF-kappaB	NF-kappaB (p65)
M00203	73 . . . 83(94)	GATA-X	GATA binding site
M00199	2077 . . . 2069(94)	AP-1	AP-1 binding site
	2069 . . . 2077(91)
	1994 . . . 2002(90)
M00174	2068 . . . 2078(94)	AP-1	activator protein 1
	1436 . . . 1446(90)
M00075	895 . . . 886(94)	GATA-1	GATA-binding factor 1
	2169 . . . 2178(93)
	1091 . . . 1082(93)
	1981 . . . 1972(92)
	2016 . . . 2025(90)
M00053	442 . . . 433(94)	c-Rel	c-Rel
M00241	2080 . . . 2087(94)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00076	716 . . . 707(94)	GATA-2	GATA-binding factor 2
	2212 . . . 2221(93)
	1609 . . . 1618(92)
	957 . . . 966(92)
	895 . . . 886(91)
	1981 . . . 1972(91)
M00106	627 . . . 636(94)	CDP	cut-like homeodomain protein
M00033	731 . . . 718(94)	p300	p300
M00227	2149 . . . 2141(94)	v-Myb	v-Myb
M00158	295 . . . 282(93)	COUP-TF	COUP/HNF-4 heterodimer
M00162	2080 . . . 2093(93)	Oct-1	octamer-binding factor 1
M00278	2213 . . . 2221(93)	Lmo2	complex of Lmo2 bound to Tal-1, E2A proteins, and
			GATA-1, half-site 2
M00134	280 . . . 298(93)	HNF-4	hepatic nuclear factor 4
M00032	226 . . . 235(93)	c-Ets-1(p54)	c-Ets-1(p54)
M00172	1436 . . . 1446(92)	AP-1	activator protein 1
	2068 . . . 2078(90)
M00117	1089 . . . 1076(92)	C/EBPbeta	CCAAT/enhancer binding protein beta
M00223	520 . . . 512(92)	STATx	signal transducers and activators of transcription
M00042	1911 . . . 1902(92)	Sox-5	Sox-5
	622 . . . 631(91)
M00073	13 . . . 23(92)	deltaEF1	deltaEF1
M00188	1436 . . . 1446(91)	AP-1	activator protein 1
	2068 . . . 2078(90)
M00254	2111 . . . 2100(91)	CCAAT	cellular and viral CCAAT box
M00099	2093 . . . 2078(91)	S8	S8
	2076 . . . 2091(90)
	1417 . . . 1432(90)
M00289	1657 . . . 1669(91)	HFH-3	HNF-3/Fkh Homolog 3 (= Freac-6)
	357 . . . 369(91)
M00147	752 . . . 743(91)	HSF2	heat shock factor 2
	142 . . . 133(90)
M00208	443 . . . 432(90)	NF-kappaB	NF-kappaB binding site
M00217	115 . . . 108(90)	USF	USF binding site
M00183	1333 . . . 1342(90)	c-Myb	c-Myb
M00173	1436 . . . 1446(90)	AP-1	activator protein 1
M00240	217 . . . 211(90)	Nkx-2.5	homeo domain factor Nkx-2.5/Csx, tinman homolog
M00184	1965 . . . 1974(90)	MyoD	myoblast determining factor
	247 . . . 256(90)
M00087	954 . . . 965(90)	Ik-2	Ikaros 2
M00128	70 . . . 82(90)	GATA-1	GATA-binding factor 1
M00145	1347 . . . 1362(90)	Brn-2	POU factor Brn-2
M00194	444 . . . 431(90)	NF-kappaB	NF-kappaB

REFERENCES

• 1. Baek, K. H., Mondoux, M. A., Jaster, R., Fire-Levin, E., and D'Andrea, A. D. (2001). DUB-2A, a new member of the DUB subfamily of hematopoietic deubiquitinating enzymes, Blood 98, 636-42.
• 2. Jaster, R., Baek, K. H., and D'Andrea, A. D. (1999). Analysis of cis-acting sequences and trans-acting factors regulating the interleukin-3 response element of the DUB-1 gene, Biochim Biophys Acta 1446, 308-16.
• 3. Jaster, R., Zhu, Y., Pless, M., Bhattacharya, S., Mathey-Prevot, B., and D'Andrea, A. D. (1997). JAK2 is required for induction of the murine DUB-1 gene, Mol Cell Biol 17, 3364-72.
• 4. Migone, T. S., Humbert, M., Rascle, A., Sanden, D., D'Andrea, A., Johnston, J. A., Baek, K. H., Mondoux, M. A., Jaster, R., Fire-Levin, E., et al. (2001). The deubiquitinating enzyme DUB-2 prolongs cytokine-induced signal transducers and activators of transcription activation and suppresses apoptosis following cytokine withdrawal, Blood 98, 1935-41.
• 5. Zhu, Y., Carroll, M., Papa, F. R., Hochstrasser, M., and D'Andrea, A. D. (1996a). DUB-1, a deubiquitinating enzyme with growth-suppressing activity, Proc Natl Acad Sci USA 93, 3275-9.
• 6. Zhu, Y., Lambert, K., Corless, C., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., and D'Andrea, A. D. (1997). DUB-2 is a member of a novel family of cytokine-inducible deubiquitinating enzymes, J Biol Chem 272, 51-7.

7. Zhu, Y., Pless, M., Inhom, R., Mathey-Prevot, B., and D'Andrea, A. D. (1996b). The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor, Mol Cell Biol 16, 4808-17.

Nucleotide sequence for hDUB4.1a
atgaccctgcaacagagcatgcccttctgcattgagcatgcaatcatgaa
tcacaggcggaggaactgcgagagtgcctacgttagcccaaggcctgacc
cgacgatcccagggaccctcgacctaactggccccgcctcccgggcccca
aacccggactcggcccccccgaagctccggatcctggggcccgcccctgg
ccccgcgtcggaagaccatgggctcgctcctgggccttcctcaaaccctc
cgcagtccaggcccggcttcctccaggtctccaggcaacgctgcggctcc
gcccacgtcatggcgcccgaggagaacgcggggacagaactctggctgca
gggtttcgagcgccgcttcctggcggcgcgctcactgcgctccttcccct
ggcagagcttagaggcaaagttaagagactcatcagattctgagctgctg
cgggatattttgcagaagactgtgaagcatcccgtgtgtgtgaagcaccc
gccatcagtcaagtatgcccggtgctttctctcagaactcatcaaaaagg
gtgcatctgtggtcaccagcagcacgagggctgtccacacggagcctttg
gacgagctgtacgaggtgctggcggagactctgatggccaaggagtccac
ccagggccaccggagctatttgctgccctcgggaggctcgttcacacttt
ccgagatcacagccatcatctcccatggtactacaggcctggtcacatgg
gacgccaccctctaccttgcagaatgggccatcgagaacccagcagcctt
cactaacaggggtgtcctagagcttggcagtggcgctggcctcacaggcc
tggccatctgcaagatgtgtcgcccccaggcatacatcttcagcgactgt
cacagccgggtcctcgagcagctccgagggaatgtccttctcaatggcct
ctcattagaggcagacatcactgccaacttagacgccccaggagaccaca
ggagaaaaacaaccacttctgggacgaggacagggcccttgagaaaaggt
ggtgtttggctgggccaccgaaaacccctcacccctgccagcacactcag
tcccctctctggtggaacagagctctgcctgtggccctgggtcccagccc
tgaaacccacaggtccagcggtggccagggacacaggcccacccctgcaa
gccagcagaccaaacggcagacacctgaaacaagaagttcacgacgtgct
gtattgcccagaagccatcgtgtcactggtcggggtcctgcggaggctgg
ctgcctgccgggagcacaagcaggctcctgaggtctacctggcctttacc
gtccgcaacccagagacgtgccagctgttcaccaccgagctagagatagc
gtctttctgcaacctgcggtcccagcagaaaaaccttgtgatccttgttc
cagtcgacatggaggacgactcactctacttgggaggtgagtggcagttc
aaccacttttcaaaactcacatcttctcggccagatgcagcttttgctga
aatccagcgtacttctctccctgagaagtcaccactctcatgtgagaccc
gtgtcgacctctgtgatgatttggctcctgtggcaagacagcttgctccc
agggagaagcctcctctgagtagcaggagacctgctgcggtgggggctgg
gctccagaatatgggaaatacctgctacgtgaacgcttccctgcagtgcc
tgacatacaaaccgccacttgccaactacatgctgttccgggagcactct
caaacgtgtcatcgtcacaagggctgcatgctctgtactatgcaagctca
catcacaagggccctccacattcctggccatgtcatccagccctcacagg
cattggctgctggcttccatagaggcaagcaggaagatgcccatgaattt
ctcatgttcactgtggatgccatgagaaaggcatgccttcccgggcacaa
gcaggtagatcgtcactctaaggacaccaccctcatccaccaaatatttg
gaggctactggagatctcaaatcaagtgtctccactgccacggcatttca
gacacttttgacccttacctggacatcgccctggatatccaggcagctca
gagtgtccagcaagctttggaacagttggtgaagcccgaagaactcaatg
gagagaatgcctatcattgtggtgtttgtctccagagggcgccggcctcc
aagacgttaactttacacaactctgccaaggtcctcatccttgtattgaa
gagattccccgatgtcacaggcaacaaaattgccaagaatgtgcaatatc
ctgagtgccttgacatgcagccatacatgtctcagcagaacacaggacct
ctcgtctatgtcctctatgctgtgctggtccacgctgggtggagttgtca
caacggacattactcctcttatgtcaaagctcaagaaggccagtggtata
aaatggatgatgccgaggtcaccgcctctagcatcacttctgtcctgagt
caacaggcctacgtcctcttttacatccagaagagtgaatgggaaagaca
cagtgagagtgtgtcaagaggcagggaaccaagagcccttggcgtagaag
acacagacaggcgagcaacgcaaggagagctcaagagagaccacccctgc
ctccaggcccccgagttggacgagcacttggtggaaagagccactcagga
aagcaccttagaccactggaaattccttcaagagcaaaacaaaacgaagc
ctgagttcaacgtcagaagagtcgaaggtacggtgcctcccgacgtactt
gtgattcatcaatcaaaatacaagtgtcggatgaagaaccatcatcctga
acagcaaagctccctgctaaacctctcttcgacgaccccgacagatcagg
agtccatgaacactggcacactcgcttccctacgagggaggaccaggaga
tccaaagggaagaacaaacacagcaagagggctctgcttgtgtgccagtg
a
hDUB4.1a deduced polypeptide sequence
MTLQQSMPFCIEHAIMNHRRRNCESAYVSPRPDPTIPGTLDLTGPASRAP
NPDSAPPKLRILGPAPGPASEDHGLAPGPSSNPPQSRPGFLQVSRQRCGS
AHVMAPEENAGTELWLQGFERRFLAARSLRSFPWQSLEAKLRDSSDSELL
RDILQKTVKHPVCVKHPPSVKYARCFLSELIKKGASVVTSSTRAVHTEPL
DELYEVLAETLMAKESTQGHRSYLLPSGGSFTLSEITAIISHGTTGLVTW
DATLYLAEWAIENPAAFTNRGVLELGSGAGLTGLAICKMCRPQAYIFSDC
HSRVLEQLRGNVLLNGLSLEADITANLDAPGDHRRKTTTSGTRTGPLRKG
GVWLGHRKPLTPASTLSPLSGGTELCLWPWVPALKPTGPAVARDTGPPLQ
ASRPNGRHLKQEVHDVLYCPEAIVSLVGVLRRLAACREHKQAPEVYLAFT
VRNPETCQLFTTELEIASFCNLRSQQKNLVILVPVDMEDDSLYLGGEWQF
NHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQLAP
REKPPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYKPPLANYMLFREHS
QTCHRHKGCMLCTMQAHITRALHIPGHVIQPSQALAAGFHRGKQEDAHEF
LMFTVDAMRKACLPGHKQVDRHSKDTTLIHQIFGGYWRSQIKCLHCHGIS
DTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPAS
KTLTLHNSAKVLILVLKRFPDVTGNKIAKNVQYPECLDMQPYMSQQNTGP
LVYVLYAVLVHAGWSCHNGHYSSYVKAQEGQWYKMDDAEVTASSITSVLS
QQAYVLFYIQKSEWERHSESVSRGREPRALGVEDTDRRATQGELKRDHPC
LQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRRVEGTVPPDVL
VIHQSKYKCRMKNHHPEQQSSLLNLSSTTPTDQESMNTGTLASLRGRTRR
SKGKNKHSKRALLVCQ
Nucleotide sequence for hDUB4.1b
atggaggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggccagatgcagcttttgctgaaatccagc
gtacttctctccctgagaagtcaccactctcatgtgagacccgtgtcgac
ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaa
gcctcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgtgaacgcttccctgcagtgcctgacatac
aaaccgccacttgccaactacatgctgttccgggagcactctcaaacgtg
tcatcgtcacaagggctgcatgctctgtactatgcaagctcacatcacaa
gggccctccacattcctggccatgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagatgcccatgaatttctcatgtt
cactgtggatgccatgagaaaggcatgccttcccgggcacaagcaggtag
atcgtcactctaaggacaccaccctcatccaccaaatatttggaggctac
tggagatctcaaatcaagtgtctccactgccacggcatttcagacacttt
tgacccttacctggacatcgccctggatatccaggcagctcagagtgtcc
agcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat
gcctatcattgtggtgtttgtctccagagggcgccggcctccaagacgtt
aactttacacaactctgccaaggtcctcatccttgtattgaagagattcc
ccgatgtcacaggcaacaaaattgccaagaatgtgcaatatcctgagtgc
cttgacatgcagccatacatgtctcagcagaacacaggacctctcgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacaacggac
attactcctcttatgtcaaagctcaagaaggccagtggtataaaatggat
gatgccgaggtcaccgcctctagcatcacttctgtcctgagtcaacaggc
ctacgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagcccttggcgtagaagacacagac
aggcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggc
ccccgagttggacgagcacttggtggaaagagccactcaggaaagcacct
tagaccactggaaattccttcaagagcaaaacaaaacgaagcctgagttc
aacgtcagaagagtcgaaggtacggtgcctcccgacgtacttgtgattca
tcaatcaaaatacaagtgtcggatgaagaaccatcatcctgaacagcaaa
gctccctgctaaacctctcttcgacgaccccgacagatcaggagtccatg
aacactggcacactcgcttccctacgagggaggaccaggagatccaaagg
gaagaacaaacacagcaagagggctctgcttgtgtgccagtga
hDUB4.1b deduced polypeptide sequence
MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVD
LCDDLAPVARQLAPREKPPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTY
KPPLANYMLFREHSQTCHRHKGCMLCTMQAHITRALHIPGHVIQPSQALA
AGFHRGKQEDAHEFLMFTVDAMRKACLPGHKQVDRHSKDTTLIHQIFGGY
WRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGEN
AYHCGVCLQRAPASKTLTLHNSAKVLILVLKRFPDVTGNKIAKNVQYPEC
LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYSSYVKAQEGQWYKMD
DAEVTASSITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGVEDTD
RRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEF
NVRRVEGTVPPDVLVIHQSKYKCRMKNHHPEQQSSLLNLSSTTPTDQESM
NTGTLASLRGRTRRSKGKNKHSKRALLVCQ
Nucleotide sequence for hDUB4.2a
atgggaaatacctgctacgtgaacgcttccttgcagtgcctgacatacac
accgccccttgccaactacatgctgtcccgggagcactctcaaacgtgtc
atcgtcacaagggctgcatgctctgtactatgcaagctcacatcacacgg
gccctccacaatcctggccacgtcatccagccctcacaggcattggctgc
tggcttccatagaggcaagcaggaagatgcccatgaatttctcatgttca
ctgtggatgccatgaaaaaggcatgccttcccaggcacaagcaggtagat
catcactctaaggacaccaccctcatccaccaaatatttggaggctactg
gagatctcaaatcaagtgtctccactgccacggcatttcagacacttttg
acccttacctggacatcgccctggatatccaggcagctcagagtgtccag
caagctttggaacagttggtgaagcccgaagaactcaatggagagaatgc
ctatcattgtggtgtttgtctccagagggcgccggcctccaagacgttaa
ctttacacacctctgccaaggtcctcatccttgtattgaagagattctcc
gatgtcacaggcaacaagattgccaagaatgtgcaatatcctgagtgcct
tgacatgcagccatacatgtctcagcagaacacaggacctcttgtctatg
tcctctatgctgtgctggtccacgctgagtggagttgtcacaacggacat
tacttctcttatgtcaaagctcaagaaggccagtggtataaaatggatga
tgccgaggtcaccgccgctagcatcacttctgtcctgagtcaacaggcct
acgtcctcttttacatccagaagagtgaatgggaaagacatagtgagagt
gtgtcaagaggcagggaaccaagagcccttggcgcagaagacacagacag
gcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggccc
ccgagttggacgagcacttggtggaaagagccactcaggaaagcacctta
gaccactggaaattccttcaagagcaaaacaaaacgaagcctgagttcaa
cgtcagaaaagtcaaaggtacagtgatcaaagttgaccagccccagagga
aagctgcccagggcacaactcagggctccgtagaaccacagaatcttggg
cgcaaccctgctcaagcacccaaatgtgcatacgaacagggtctccgtgt
gacggaacatgtccactttcggcagcattacaattttggcaccaaatgtg
ctaactgcaattccaccatacaatgcgtaactggaaatggaggcaacatc
gccgatcctgaacgatcgatgcgagaatccaggatatgcacggcttattt
tggccttttcccactgaaacaagggccagtattaaaaatgcagaaaaacc
ttgtgatcctcgttccagtcgacatggaggacgactcactctacttggga
ggtgagtggcagttcaaccacttttcaaaactcacatcttctcggcccga
tgcagcttttgctgaaatccagcggacttctctccctgagaagtcaccac
tctcatgtgagacccgtgtcgacctctgtgatgatttggctcctgtggca
agacagcttgctcccagggagaagcttcctctgagtagcaggagacctgc
tgcggtgggggctgggctccagaatatgggaaatacctgctacgtgaacg
cttccttgcagtgcctgacatacacaacgccccttgccaactacatgctg
tcccgggagcactctcaaacgtgtcatcgtcacaagggctgcatgctctg
tactatgcaagctcacatcacacgggccctccacaatcctggccacgtca
tccagccctcacaggcattggctgctggcttccatagaggcaagcaggaa
gatgcccatgaatttctcatgttcactgtggatgccatgaaaaaggcatg
ccttcccgggcacaagcaggtagatcatcactctaaggacaccaccctca
tccaccaaatatttggaggctactggagatctcaaatcaagtgtctccac
tgccacggcatttcagacacttttgacccttacctggacatcgccctaga
tatccaggcagctcagagtgtccagcaagctttggaacagttggtgaagc
ccgaagaactcaatggagagaatgcctatcattctggtgtttgtctccag
agggcgccggcctccaagacgttaactttacacacctctgccaaggtcct
catccttgtattgaagagattctccgatgtcacaggcaacaagattgcca
agaatgtgcaatatcctgagtgccttgacatgcagccatacatgtctcag
cagaacacaggacctcttgtctatgtcctctatgctgtgctggtccacgc
tgggtggagttgtcacaacggacattacttctcttatgtcaaagctcaag
aaggccagtggtataaaatggatgatgccgaggtcaccgccgctagcatc
acttctgccctgagtcaacaggcctacgtcctcttttacatccagaagag
tgaatgggaaagacacagtgagagtgtgtcaagaggcagggaaccaagag
cccttggcacagaagacacagacaggcgagcaacgcaaggagagctcaag
agagaccacccctgcctccaggcccccgagttggacgagcacttggtgga
aagagccactcaggaaagcaccttagaccactggaaattccttcaagagc
aaaacaaaacgaagcctgagttcaacgtcagaaaagtcgaaggtaccctg
cctcccgacgtacttgtgattcatcaatcaaaatacaagtgtgggatgaa
gaaccatcatcctgaacagcaaagctccctgctaaacctctcttcgtcga
ccccgacacatcaggagtccatgaacactggcacactcgcttccctgcga
gggagggccaggagatccaaagggaagaacaaacacagcaagagggctct
gcttgtgtgccagtga
hDUB4.2a deduced polypeptide sequence
MGNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRHKGCMLCTMQAHITR
ALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPRHKQVD
HHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQ
QALEQLVKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFS
DVTGNKIAKNVQYPECLDMQPYMSQQNTGPLVYVLYAVLVHAEWSCHNGH
YFSYVKAQEGQWYKMDDAEVTAASITSVLSQQAYVLFYIQKSEWERHSES
VSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTL
DHWKFLQEQNKTKPEFNVRKVKGTVIKVDQPQRKAAQGTTQGSVEPQNLG
RNPAQAPKCAYEQGLRVTEHVHFRQHYNFGTKCANCNSTIQCVTGNGGNI
ADPERSMRESRICTAYFGLFPLKQGPVLKMQKNLVILVPVDMEDDSLYLG
GEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVA
RQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTTPLANYML
SREHSQTCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQE
DAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQIKCLH
CHGISDTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGENAYHSGVCLQ
RAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPECLDMQPYMSQ
QNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTAASI
TSALSQQAYVLFYIQKSEWERHSESVSRGREPRALGTEDTDRRATQGELK
RDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTL
PPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSSTPTHQESMNTGTLASLR
GRARRSKGKNKHSKRALLVCQ
Nucleotide sequence for hDUB4.2b
atggaggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggcccgatgcagcttttgctgaaatccagc
ggacttctctccctgagaagtcaccactctcatgtgagacccgtgtcgac
ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaa
gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgtgaacgcttccttgcagtgcctgacatac
acaacgccccttgccaactacatgctgtcccgggagcactctcaaacgtg
tcatcgtcacaagggctgcatgctctgtactatgcaagctcacatcacac
gggccctccacaatcctggccacgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagatgcccatgaatttctcatgtt
cactgtggatgccatgaaaaaggcatgccttcccgggcacaagcaggtag
atcatcactctaaggacaccaccctcatccaccaaatatttggaggctac
tggagatctcaaatcaagtgtctccactgccacggcatttcagacacttt
tgacccttacctggacatcgccctagatatccaggcagctcagagtgtcc
agcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat
gcctatcattctggtgtttgtctccagagggcgccggcctccaagacgtt
aactttacacacctctgccaaggtcctcatccttgtattgaagagattct
ccgatgtcacaggcaacaagattgccaagaatgtgcaatatcctgagtgc
cttgacatgcagccatacatgtctcagcagaacacaggacctcttgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacaacggac
attacttctcttatgtcaaagctcaagaaggccagtggtataaaatggat
gatgccgaggtcaccgccgctagcatcacttctgccctgagtcaacaggc
ctacgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagcccttggcacagaagacacagac
aggcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggc
ccccgagttggacgagcacttggtggaaagagccactcaggaaagcacct
tagaccactggaaattccttcaagagcaaaacaaaacgaagcctgagttc
aacgtcagaaaagtcgaaggtaccctgcctcccgacgtacttgtgattca
tcaatcaaaatacaagtgtgggatgaagaaccatcatcctgaacagcaaa
gctccctgctaaacctctcttcgtcgaccccgacacatcaggagtccatg
aacactggcacactcgcttccctgcgagggagggccaggagatccaaagg
gaagaacaaacacagcaagagggctctgcttgtgtgccagtga
hDUB4.2b deduced polypeptide sequence
MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVD
LCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTY
TTPLANYMLSREHSQTCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALA
AGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGY
WRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGEN
AYHSGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPEC
LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMD
DAEVTAASITSALSQQAYVLFYIQKSEWERHSESVSRGREPRALGTEDTD
RRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEF
NVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSSTPTHQESM
NTGTLASLRGRARRSKGKNKHSKRALLVCQ
Nucleotide sequence for hDUB4.3
atggaggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggcccgatgcagcttttgctgaaatccagc
ggacttctctccctgagaagtcaccactctcatgtgagacccgtgtcgac
ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaa
gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgtgaacgcttccttgcagtgcctgacatac
acaccgccccttgccaactacatgctgtcccgggagcactctcaaacgtg
tcatcgtcacaagggctgcatgctctgtacgatgcaagctcacatcacac
gggccctccacaatcctggccacgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagatgcccatgaatttctcatgtt
cactgtggatgccatgaaaaaggcatgccttcccgggcacaagcaggtag
atcatcactctaaggacaccaccctcatccaccaaatatttggaggctac
tggagatctcaaatcaagtgtctccactgccacggcatttcagacacttt
tgacccttacctggacatcgccctggatatccaggcagctcagagtgtcc
agcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat
gcctatcattgtggtgtttgtctccagagggcgccggcctccaagacgtt
aactttacacacctctgccaaggtcctcatccttgtattgaagagattct
ccgatgtgacaggcaacaagattgccaagaatgtgcaatatcctgagtgc
cttgacatgcagccatacatgtctcagcagaacacaggacctcttgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacaacggac
attacttctcttatgtcaaagctcaagaaggccaatggtataaaatggat
gatgccgaggtcaccgccgctagcatcacttctgtcctgagtcaacaggc
ctacgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagcccttggcgcagaagacacagac
aggcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggc
ccccgagttggacgagcacttggtggaaagagccactcaggaaagcacct
tagaccgctggaaattccttcaagagcaaaacaaaacgaagcctgagttc
aacgtcagaaaagtcgaaggtaccctgcctcccgacgtacttgtgattca
tcaatcaaaatacaagtgtgggatgaagaaccatcatcctgaacagcaaa
gctccctgctaaacctctcttcgtcgaccccgacacatcaggagtccatg
aacactggcacactcgcttccctgcgagggagggccaggagatccaaagg
gaagaacaaacacagcaagagggctctgcttgtgtgccagtga
hDUB4.3 deduced polypeptide sequence
MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVD
LCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTY
TPPLANYMLSREHSQTCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALA
AGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGY
WRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGEN
AYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPEC
LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMD
DAEVTAASITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTD
RRATQGELKRDHPCLQAPELDEHLVERATQESTLDRWKFLQEQNKTKPEF
NVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSSTPTHQESM
NTGTLASLRGRARRSKGKNKHSKRALLVCQ
Nucleotide sequence for hDUB4.5
atgcgccagagagctcgtcatttgaagactctctcggaagggatagcgtc
tttctgcaacctgcggtcccagcagaaaaaccttgtgatccttgttccag
tcgacatggaggaagactcactctacttgggaggtgagtggcagttcaac
cacttttcaaaactcacatcttctcggcccgatgcagcttttgctgaaat
ccagcggacttctctccctgagaagtcaccactctcatgtgagacccgtg
tcgacctctgtgatgatttggctcctgtggcaagacagcttgctcccagg
gagaagcttcctctgagtaacaggagacctgctgcggtgggggctgggct
ccagaatatgggaaatacctgctacgtgaacgcttccttgcagtgcctga
catacacaccgccccttgccaactacatgctgtcccgggagcactctcaa
acgtgtcatcgtcacaagggctgcatgctctgtacgatgcaagctcacat
cacacgggccctccacaatcctggccacgtcatccagccctcacaggcat
tggctgctggcttccatagaggcaagcaggaagatgcccatgaatttctc
atgttcactgtggatgccatgaaaaaggcatgccttcccgggcacaagca
ggtggatcatcactctaaggacaccaccctcatccaccaaatatttggag
gctactggagatctcaaatcaagtgtctccactgccacggcatttcagac
acttttgacccttacctggacatcgccctggatatccaggcagctcagag
tgtccagcaagctttggaacagttggtgaagcccgaagaactcaatggag
agaatgcctatcattgtggtgtttgtctccagagggcgccggcctccaag
acgttaactttacacacctctgccaaggtcctcatccttgtattgaagag
attctccgatgtcacaggcaacaagattgacaagaatgtgcaatatcctg
agtgccttgacatgaagctatacatgtctcagacgaactcaggacctctc
gtctatgtcctctatgctgtgctggtccacgctgggtggagttgtcacaa
cggacattacttctcttatgtcaaagctcaagaaggccagtggtataaaa
tggatgatgccgaggtcaccgcctctagcatcacttctgtcctgagtcaa
caggcctacgtcctcttttacatccagaagagtgaatgggaaagacacag
tgagagtgtgtcaagaggcagggaaccaagagcccttggcgcagaagaca
cagacaggcgagcaacgcaaggagagctcaagagagaccacccctgcctc
caggcccccgagttggacgagcacttggtggaaagagccactcaggaaag
caccttagaccactggaaattccttcaagagcaaaacaaaacgaagcctg
agttcaacgtcagaaaagtcgaaggtaccctgcctcccgacgtacttgtg
attcatcaatcaaaatacaagtgtgggatgaagaaccatcatcctgaaca
gcaaagctccctgctaaacctctcttcgacgaccccgacacatcaggagt
ccatgaacactggcacactcgcttccctgcgagggagggccaggagatcc
aaagggaagaacaaacacagcaagagggctctgcttgtgtgccagtggtc
tcagtggaagtaccgacccaca
hDUB4.5 deduced polypeptide sequence
MRQRARHLKTLSEGIASFCNLRSQQKNLVILVPVDMEEDSLYLGGEWQFN
HFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQLAPR
EKLPLSNRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQ
TCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFL
MFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISD
TFDPYLDIALDIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPASK
TLTLHTSAKVLILVLKRFSDVTGNKIDKNVQYPECLDMKLYMSQTNSGPL
VYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASSITSVLSQ
QAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCL
QAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLV
IHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRS
KGKNKHSKRALLVCQWSQWKYRPT
Nucleotide sequence for hDUB4.6
atggaggacg actcactcta cttgggaggt gagtggcagt
tcaaccactt ttcaaaactc acatcttctc ggcccgatgc
agcttttgct gaaatccagc ggacttctct ccctgagaag
tcaccactct catgtgagac ccgtgtcgac ctctgtgatg
atttggctcc tgtggcaaga cagcttgctc ccagggagaa
gcttcctctg agtagcagga gacctgctgc ggtgggggct
gggctccaga atatgggaaa tacctgctac gtgaacgctt
ccttgcagtg cctgacatac acaccgcccc ttgccaacta
catgctgtcc cgggagcact ctcaaacgtg tcatcgtcac
aagggctgta tgctctgtac gatgcaagct cacatcacac
gggccctcca caatcctggc cacgtcatcc agccctcaca
ggcattggct gctggcttcc atagaggcaa gcaggaagat
gcccatgaat ttctcatgtt cactgtggat gccatgaaaa
aggcatgcct tcccgggcac aagcaggtgg atcatcactc
taaggacacc accctcatcc accaaatatt tggaggctac
tggagatctc aaatcaagtg tctccactgc cacggcattt
cagacacttt tgacccttac ctggacatcg ccctggatat
ccaggcagct cagagtgtcc agcaagcttt ggaacagttg
gtgaagcccg aagaactcaa tggagagaat gcctatcatt
gtggtgtttg tctccagagg gcgccggcct ccaagacgtt
aactttacac acctctgcca aggtcctcat ccttgtattg
aagagattct ccgatgtcac aggcaacaag attgccaaga
atgtgcaata tcctgagtgc cttgacatgc agccatacat
gtctcagacg aacacaggac ctctcgtcta tgtcctctat
gctgtgctgg tccacgctgg gtggagttgt cacaacggac
attacttctc ttatgtcaaa gctcaagaag gccagtggta
taaaatggat gatgccgagg tcaccgcctc tagcatcact
tctgtcctga gtcaacaggc ctacgtcctc ttttacatcc
agaagagtga atgggaaaga cacagtgaga gtgtgtcaag
aggcagggaa ccaagagccc ttggcgcaga agacacagac
aggcgagcaa cgcaaggaga gctcaagaga gaccacccct
gcctccaggc ccccgagttg gacgagcact tggtggaaag
agccactcag gaaagcacct tagaccactg gaaattcctt
caagagcaaa acaaaacgaa gcctgagttc aacgtcagaa
aagtcgaagg taccctgcct cccgacgtac ttgtgattca
tcaatcaaaa tacaagtgtg ggatgaagaa ccatcatcct
gaacagcaaa gctccctgct aaacctctct tcgacgaccc
cgacacatca ggagtccatg aacactggca cactcgcttc
cctgcgaggg agggccagga gatccaaagg gaagaacaaa
cacagcaaga gggctctgct tgtgtgccag tga
hDUB4.6 deduced polypeptide sequence
MEDDSLYLGG EWQFNHFSKL TSSRPDAAFA EIQRTSLPEK
SPLSCETRVD LCDDLAPVAR QLAPREKLPL SSRRPAAVGA
GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH
KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED
AHEFLMFTVD AMKKACLPGH KQVDHHSKDT TLIHQIFGGY
WRSQIKCLHC HGISDTFDPY LDIALDIQAA QSVQQALEQL
VKPEELNGEN AYHCGVCLQR APASKTLTLH TSAKVLILVL
KRFSDVTGNK IAKNVQYPEC LDMQPYMSQT NTGPLVYVLY
AVLVHAGWSC HNGHYFSYVK AQEGQWYKMD DAEVTASSIT
SVLSQQAYVL FYIQKSEWER HSESVSRGRE PRALGAEDTD
RRATQGELKR DHPCLQAPEL DEHLVERATQ ESTLDHWKFL
QEQNKTKPEF NVRKVEGTLP PDVLVIHQSK YKCGMKNHHP
EQQSSLLNLS STTPTHQESM NTGTLASLRG RARRSKGKNK
HSKRALLVCQ
Nucleotide sequence for hDUB4.7
atggaggacg actcactcta cttgggtggt gagtggcagt
tcaaccactt ttcaaaactc acatcttctc ggcccgatgc
agcttttgct gaaatccagc ggacttctct ccctgagaag
tcaccactct catgtgagac ccgtgtcgac ctctgtgatg
atttggctcc tgtggcaaga cagcttgctc ccagggagaa
gcttcctctg agtagcagga gacctgctgc ggtgggggct
gggctccaga atatgggaaa tacctgctac gtgaacgctt
ccttgcagtg cctgacatac acaccgcccc ttgccaacta
catgctgtcc cgggagcact ctcaaacgtg tcatcgtcac
aagggctgca tgctctgtac tatgcaagct cacatcacac
gggccctcca caatcctggc cacgtcatcc agccctcaca
ggcattggct gctggcttcc atagaggcaa gcaggaagat
gcccatgaat ttctcatgtt cactgtggat gccatgaaaa
aggcatgcct tcccgggcac aagcaggtag atcatcactc
taaggacacc accctcatcc accaaatatt tggaggctac
tggagatctc aaatcaactg tctccactgc cacggcattt
cagacacttt tgacccttac ctggacatcg ccctggatat
ccaggcagct cagagtgtcc agcaagcttt ggaacagttg
gtgaagcccg aagaactcaa tggagagaat gcctatcatt
gtggtgtttg tctccagagg gcgccggcct ccaagacgtt
aactttacac acctctgcca aggtcctcat ccttgtattg
aagagattct ccgatgtcac aggcaacaag attgccaaga
atgtgcaata tcctgagtgc cttgacatgc agccatacat
gtctcagcag aacacaggac ctcttgtcta tgtcctctat
gctgtgctgg tccacgctgg gtggagttgt cacaacggac
attacttctc ttatgtcaaa gctcaagaag gccagtggta
taaaatggat gatgccgagg tcaccgccgc tagcatcact
tctgtcctga gtcaacaggc ctacgtcctc ttttacatcc
agaagagtga atgggaaaga cacagtgaga gtgtgtcaag
aggcagggaa ccaagagccc ttggcgcaga agacacagac
aggcgagcaa cgcaaggaga gctcaagaga gaccacccct
gcctccaggc ccccgagttg gacgagcact tggtggaaag
agccactcag gaaagcacct tagaccactg gaaattcctt
caagagcaaa acaaaacgaa gcctgagttc aacgtcagaa
aagtcgaagg taccctgcct cccgacgtac ttgtgattca
tcaatcaaaa tacaagtgtg ggatgaagaa ccatcatcct
gaacagcaaa gctccctgct aaacctctct tcgtcgaccc
cgacacatca ggaggccatg aacactggca cactcgcttc
cctgcgaggg aggaccagga gatccaaagg gaagaacaaa
cacagcaaga gggctctgct tgtgtgccag tga
hDUB4.7 deduced polypeptide sequence
MEDDSLYLGG EWQFNHFSKL TSSRPDAAFA EIQRTSLPEK
SPLSCETRVD LCDDLAPVAR QLAPREKLPL SSRRPAAVGA
GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH
KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED
AHEFLMFTVD ANKKACLPGH KQVDHHSKDT TLIHQIFGGY
WRSQINCLHC HGISDTFDPY LDIALDIQAA QSVQQALEQL
VKPEELNGEN AYHCGVCLQR APASKTLTLH TSAKVLILVL
KRFSDVTGNK IAKNVQYPEC LDMQPYNSQQ NTGPLVYVLY
AVLVHAGWSC HNGHYFSYVK AQEGQWYKMD DAEVTAASIT
SVLSQQAYVL FYIQKSEWER HSESVSRGRE PRALGAEDTD
RRATQGELKR DHPCLQAPEL DEHLVERATQ ESTLDHWKFL
QEQNKTKPEF NVRKVEGTLP PDVLVIHQSK YKCGMKNHHP
EQQSSLLNLS SSTPTHQEAM NTGTLASLRG RTRRSKGKNK
HSKRALLVCQ
Nucleotide sequence for hDUB4.8
atgcgccaga gagctcgtca tttgaagact ctctcggaag
ggatagcgtc ttgctgcaaa ctgcggtccc agcagaaaaa
ccttgtgatc cttgttccag tcgacatgga ggacgactca
ctctacttgg gaggtgagtg gcagttcaac cacttttcaa
aactcacatc ttctcggccc gatgcagctt ttgctgaaat
ccagcggact tctctccctg agaagtcacc actctcatgt
gagacccgtg tcgacctctg tgatgatttg gctcctgtgg
caagacagct tgctcccagg gagaagcttc ctctgagtag
caggagacct gctgcggtgg gggctgggct ccagaatatg
ggaaatacct gctacgtgaa cgcttccttg cagtgcctga
catacacacc gccccttgcc aactacatgc tgtcccggga
gcactctcaa acgtgtcatc gtcacaaggg ctgcatgctc
tgtacgatgc aagctcacat cacacgggcc ctccacaatc
ctggccacgt catccagccc tcacaggcat tggctgctgg
cttccataga ggcaagcagg aagatgccca tgaatttctc
atgttcactg tggatgccat gaaaaaggca tgccttcccg
ggcacaagca ggtagatcat cactctaagg acaccaccct
catccaccaa atatttggag gctactggag atctcaaatc
aagtgtctcc actgccacgg catttcagac acttttgacc
cttacctgga catcgccctg gatatccagg cagctcagag
tgtccagcaa gctttggaac agttggtgaa gcccgaagaa
ctcaatggag agaatgccta tcattgtggt gtttgtctcc
agagggcgcc ggcctccaag acgttaactt tacacacctc
tgccaaggtc ctcatccttg tattgaagag attctccgat
gtgacaggca acaagattgc caagaatgtg caatatcctg
agtgccttga catgcagcca tacatgtctc agcagaacac
aggacctctt gtctatgtcc tctatgctgt gctggtccac
gctgggtgga gttgtcacaa cggacattac ttctcttatg
tcaaagctca agaaggccaa tggtataaaa tggatgatgc
cgaggtcacc gccgctagca tcacttctgt cctgagtcaa
caggcctacg tcctctttta catccagaag agtgaatggg
aaagacacag tgagagtgtg tcaagaggca gggaaccaag
agcccttggc gcagaagaca cagacaggcg agcaacgcaa
ggagagctca agagagacca cccctgcctc caggcccccg
agttggacga gcacttggtg gaaagagcca ctcaggaaag
caccttagac cactggaaat tccttcaaga gcaaaacaaa
acgaagcctg agttcaacgt cagaaaagtc gaaggtaccc
tgcctcccga cgtacttgtg attcatcaat caaaatacaa
gtgtgggatg aagaaccatc atcctgaaca gcaaagctcc
ctgctaaacc tctcttcgtc gaccccgaca catcaggagt
ccatgaacac tggcacactc gcttccctgc gagggagggc
caggagatcc aaagggaaga acaaacacag caagagggct
ctgcttgtgt gccagtga
hDUB4.8 deduced polypeptide sequence
MRQRARHLKT LSEGIASCCK LRSQQKNLVI LVPVDMEDDS
LYLGGEWQFN HFSKLTSSRP DAAFAEIQRT SLPEKSPLSC
ETRVDLCDDL APVARQLAPR EKLPLSSRRP AAVGAGLQNM
GNTCYVNASL QCLTYTPPLA NYMLSREHSQ TCHRHKGCML
CTMQAHITRA LHNPGHVIQP SQALAAGFHR GKQEDAHEFL
MFTVDANKKA CLPGHKQVDH HSKDTTLIHQ IFGGYWRSQI
KCLHCHGISD TFDPYLDIAL DIQAAQSVQQ ALEQLVKPEE
LNGENAYHCG VCLQRAPASK TLTLHTSAKV LILVLKRFSD
VTGNKIAKNV QYPSCLDMQP YNSQQNTGPL VYVLYAVLVH
AGWSCHNGHY FSYVKAQEGQ WYKMDDAEVT AASITSVLSQ
QAYVLFYIQK SEWERHSESV SRGREPRALG AEDTDRRATQ
GELKRDHPCL QAPELDEHLV ERATQESTLD HWKFLQEQNK
TKPEFNVRKV EGTLPPDVLV IHQSKYKCGM KNHHPEQQSS
LLNLSSSTPT HQESMNTGTL ASLRGRARRS KGKNKHSKRA
LLVCQ
Nucleotide sequence for hDUB4.10
atgtgcatac gaacagggtc tccgtgtgac gtgtgtgaaa
actacagtgt gatgagcatg actggcagac agcttatcga
ttgggctccc ctcaaaatcg gttatgagca ttcaagcaca
ccgatgccca gggaacatgt ccactttcgg cagcattaca
attttggcac caaatgtgct aactgcaatt ccaccataca
atgcgtaact ggaaatggag gcaacatcgc cgatcctgaa
cgatcgatgc gagaatccag gatatgcacg gcttattttg
gccttttccc actgaaacaa gggccagtat taaaaatggt
aatttcactc ggacagagaa tcaataggct caacgtggaa
aggttatcgc tggaagggaa gaaaatacgc tgtgctaaat
actatacttc attgactatt ctcaggtcag aaagcgcact
ttcgacttct tgtccttccg tcgctgagag gatgatggca
gctgccaaaa ggatagcgtc tttctgcaac ctgcggtccc
agcagaaaaa ccttgtgatc ctcgttccag tcgacatgga
ggacgactca ctctacttgg gaggtgagtg gcagttcaac
cacttttcaa aactcacatc ttctcggccc gatgcagctt
ttgctgaaat ccagcggact tctctccctg agaagtcacc
actctcatgt gagacccgtg tcgacctctg tgatgatttg
gctcctgtgg caagacagct tgctcccagg gagaagcttc
ctctgagtag caggagacct gctgcggtgg gggctgggct
ccagaatatg ggaaatacct gctacgtgaa cgcttccttg
cagtgcctga catacacaac gccccttgcc aactacatgc
tgtcccggga gcactctcaa acgtgtcatc gtcacaaggg
ctgcatgctc tgtactatgc aagctcacat cacacgggcc
ctccacaatc ctggccacgt catccagccc tcacaggcat
tggctgctgg cttccataga ggcaagcagg aagatgccca
tgaatttctc atgttcactg tggatgccat gaaaaaggca
tgccttcccg ggcacaagca ggtagatcat cactctaagg
acaccaccct catccaccaa atatttggag gctactggag
atctcaaatc aagtgtctcc actgccacgg catttcagac
acttttgacc cttacctgga catcgcccta gatatccagg
cagctcagag tgtccagcaa gctttggaac agttggtgaa
gcccgaagaa ctcaatggag agaatgccta tcattctggt
gtttgtctcc agagggcgcc ggcctccaag acgttaactt
tacacacctc tgccaaggtc ctcatccttg tattgaagag
attctccgat gtcacaggca acaagattgc caagaatgtg
caatatcctg agtgccttga catgcagcca tacatgtctc
agcagaacac aggacctctt gtctatgtcc tctatgctgt
gctggtccac gctgggtgga gttgtcacaa cggacattac
ttctcttatg tcaaagctca agaaggccag tggtataaaa
tggatgatgc cgaggtcacc gccgctagca tcacttctgc
cctgagtcaa caggcctacg tcctctttta catccagaag
agtgaatggg aaagacacag tgagagtgtg tcaagaggca
gggaaccaag agcccttggc acagaagaca cagacaggcg
agcaacgcaa ggagagctca agagagacca cccctgcctc
caggcccccg agttggacga gcacttggtg gaaagagcca
ctcaggaaag caccttagac cactggaaat tccttcaaga
gcaaaacaaa acgaagcctg agttcaacgt cagaaaagtc
gaaggtaccc tgcctcccga cgtacttgtg attcatcaat
caaaatacaa gtgtgggatg aagaaccatc atcctgaaca
gcaaagctcc ctgctaaacc tctcttcgtc gaccccgaca
catcaggagt ccatgaacac tggcacactc gcttccctgc
gagggagggc caggagatcc aaagggaaga acaaacacag
caagagggct ctgcttgtgt gccagtga
hDUB4.10 deduced polypeptide sequence
MCIRTGSPCD VCENYSVMSM TGRQLIDWAP LKIGYEHSST
PNPRSHVHFR QHYNFGTKCA NCNSTIQCVT GNGGNIADPE
RSMRESRICT AYFGLFPLKQ GPVLKMVISL GQRINRLNVE
RLSLEGKKIR CAKYYTSLTI LRSESALSTS CPSVAERMMA
AAKRIASFCN LRSQQKNLVI LVPVDMEDDS LYLGGEWQFN
HFSKLTSSRP DAAFAEIQRT SLPEKSPLSC ETRVDLCDDL
APVARQLAPR EKLPLSSRRP AAVGAGLQNM GNTCYVNASL
QCLTYTTPLA NYMLSREHSQ TCHRHKGCML CTMQAHITRA
LHNPGHVIQP SQALAAGFHR GKQEDAHEFL NFTVDANKKA
CLPGHKQVDH HSKDTTLIHQ IFGGYWRSQI KCLHCHGISD
TFDPYLDIAL DIQAAQSVQQ ALEQLVKPEE LNGENAYHSG
VCLQRAPASK TLTLHTSAKV LILVLKRFSD VTGNKIAKNV
QYPECLDMQP YMSQQNTGPL VYVLYAVLVH AGWSCHNGHY
FSYVKAQEGQ WYKMDDAEVT AASITSALSQ QAYVLFYIQK
SEWERHSESV SRGREPRALG TEDTDRRATQ GELKRDHPCL
QAPELDEHLV ERATQESTLD HWKFLQEQNK TKPEFNVRKV
EGTLPPDVLV IHQSKYKCGM KNHHPEQQSS LLNLSSSTPT
HQESMNTGTL ASLRGRARRS KGKNKHSKRA LLVCQ
Nucleotide sequence for hDUB4.11
atgtgcatac gaacagggtc tccgtgtgac gtgtgtgaaa
actacagtgt gatgagcatg actggcagac agcttatcga
ttgggctccc ctcaaaatcg gttatgagca ttcaagcaca
ccgatgccca ggacacttta catccggcac aggaagcctt
ctgatggagc acacctggcc catgaaaaga caagggaaag
aaacggggcc aaagggaaga aaatacgctg tgctaaatac
tatacttcat tgactattct caggtcagaa agcgcacttt
cgtcttcttg tccttccgtc gcggagagga tgatggcagc
tgccaaaatc gacatggagg acgactcact ctacttggga
ggtgagtggc agttcaacca cttttcaaaa ctcacatctt
ctcggccaga tgcagctttt gctgaaatcc agcggacttc
tctccctgag aagtcaccac tctcatatga tttggctcct
gtggcaagac agcttgctcc cagggagaag cttcctctga
gtagcaggag acctgctgcg gtgggggctg ggctccagaa
tatgggaaat acctgctacg tgaacgcttc cttgcagtgc
ctgacataca caccgcccct tgccaactac atgctgtccc
gggagcactc tcaaacgtgt catcgtcaca agggctgcat
gctctgtact atgcaagctc acatcacacg ggccctccac
aatcctggcc acgtcatcca gccctcacag gcattggctg
ctggcttcca tagaggcaag caggaagatg cccatgaatt
tctcatgttc actgtggatg ccatgaaaaa ggcatgcctt
cccaggcaca agcaggtaga tcatcactct aaggacacca
ccctcatcca ccaaatattt ggaggctact ggagatctca
aatcaagtgt ctccactgcc acggcatttc agacactttt
gacccttacc tggacatcgc cctggatatc caggcagctc
agagtgtcca gcaagctttg gaacagttgg tgaagcccga
agaactcaat ggagagaatg cctatcattg tggtgtttgt
ctccagaggg cgccggcctc caagacgtta actttacaca
cctctgccaa ggtcctcatc cttgtattga agagattctc
cgatgtcaca ggcaacaaga ttgccaagaa tgtgcaatat
cctgagtgcc ttgacatgca gccatacatg tctcagcaga
acacaggacc tcttgtctat gtcctctatg ctgtgctggt
ccacgctgag tggagttgtc acaacggaca ttacttctct
tatgtcaaag ctcaagaagg ccagtggtat aaaatggatg
atgccgaggt caccgccgct agcatcactt ctgtcctgag
tcaacaggcc tacgtcctct tttacatcca gaagagtgaa
tgggaaagac atagtgagag tgtgtcaaga ggcagggaac
caagagccct tggcgcagaa gacacagaca ggcgagcaac
gcaaggagag ctcaagagag accacccctg cctccaggcc
cccgagttgg acgagcactt ggtggaaaga gccactcagg
aaagcacctt agaccactgg aaattccttc aagagcaaaa
caaaacgaag cctgagttca acgtcagaaa agtcaaaggt
accctgcctc ccgacgtact tgtgattcat caatcaaaat
acaagtgtgg gatgaagaac catcatcctg aacagcaaag
ctccctgcta aacctctctt cgtcgacccc gacacatcag
gagtccatga acactggcac actcgcttcc ctgcgaggga
gggccaggag atccaaaggg aagaacaaac acagcaagag
ggctctgctt gtgtgccagt ga
hDUB4.11 deduced polypeptide sequence
MCIRTGSPCD VCENYSVMSM TGRQLIDWAP LKIGYEHSST
PMPRTLYIRH RKPSDGAHLA HEKTRERNGA KGKKIRCAKY
YTSLTILRSE SALSSSCPSV AERMMAAAKI DMEDDSLYLG
GEWQFNHFSK LTSSRPDAAF AEIQRTSLPE KSPLSYDLAP
VARQLAPREK LPLSSRRPAA VGAGLQNMGN TCYVNASLQC
LTYTPPLANY MLSREHSQTC HRHKGCNLCT MQAHITRALH
NPGHVIQPSQ ALAAGFHRGK QEDAHEFLMF TVDAMKKACL
PRHKQVDHHS KDTTLIHQIF GGYWRSQIKC LHCHGISDTF
DPYLDIALDI QAAQSVQQAL EQLVKPEELN GENAYHCGVC
LQRAPASKTL TLHTSAKVLI LVLKRFSDVT GNKIAKNVQY
PECLDMQPYN SQQNTGPLVY VLYAVLVHAE WSCHNGHYFS
YVKAQEGQWY KMDDAEVTAA SITSVLSQQA YVLFYIQKSE
WERHSESVSR GREPRALGAE DTDRRATQGE LKRDHPCLQA
PELDEHLVER ATQESTLDHW KFLQEQNKTK PEFNVRKVKG
TLPPDVLVIH QSKYKCGMKN HHPEQQSSLL NLSSSTPTHQ
ESMNTGTLAS LRGRARRSKG KNKHSKRALL VCQ
Nucleotide sequence for hDUB8.1
atgggggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggccagatgcagcttttgctgaaatccagc
ggacttctctccctgagaagtcaccactctcatctgagacccgtgtcgac
ctctgtgatgatttggctcctgtggcaagacagctcgctcccagggagaa
gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgagaacgcttccctgcagtgcctgacatac
acactgccccttgccaactacatgctgtcccgggagcactctcaaacatg
tcagcgtcccaagtgctgcatgctctgtactatgcaagctcacatcacat
gggccctccacagtcctggccatgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagatgtccatgaatttctcatgtt
cactgtggatgccatgaaaaaggcatgccttcccggccacaagcaggtag
atcatcactgcaaggacaccaccctcatccaccaaatatttggaggctgc
tggagatctcaaatcaagtgtctccactgccacgggatttcagacacttt
tgacccttacctggacatcgccctggatatccaggcagctcagagtgtca
agcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat
gcctatcattgcggtctttgtctccagagggcgccggcctccaacacgtt
aactttacacacttctgccaaggtcctcatccttgtcttgaagagattct
ccgatgtcgcaggcaacaaacttgccaagaatgtgcaatatcctgagtgc
cttgacatgcagccatacatgtctcagcagaacacaggacctcttgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacgacggac
attacttctcctatgtcaaagctcaagaagtccagtggtataaaatggat
gatgccgaggtcactgtctgtagcatcatttctgtcctgagtcaacaggc
ctatgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagccctcggcgctgaagacacagac
aggcgagcaaagcaaggagagctcaagagagaccacccctgcctccaggc
acccgagttggacgagcacttggtggaaagagccactcaggaaagcacct
tagaccactggaaattcctgcaagagcaaaacaaaacgaagcctgagttc
aacgtcggaaaagtcgaaggtaccctgcctcccaacgcacttgtgattca
tcaatcaaaatacaagtgtgggatgaaaaaccatcatcctgaacagcaaa
gctccctgctaaacctctcttcgacgacccggacagatcaggagtccatg
aacactggcacactcgcttctctgcaagggaggaccaggagagccaaagg
gaagaacaaacacagcaagagggctctgcttgtgtgccagtga
hDUB8.1 deduced polypeptide sequence
MGDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSETRVD
LCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYENASLQCLTY
TLPLANYMLSREHSQTCQRPKCCMLCTMQAHITWALHSPGHVIQPSQALA
AGFHRGKQEDVHEFLMFTVDAMKKACLPGHKQVDHHCKDTTLIHQIFGGC
WRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVKQALEQLVKPEELNGEN
AYHCGLCLQRAPASNTLTLHTSAKVLILVLKRFSDVAGNKLAKNVQYPEC
LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEVQWYKMD
DAEVTVCSIISVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTD
RRAKQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEF
NVGKVEGTLPPNALVIHQSKYKCGMKNHHPEQQSSLLNLSSTTRTDQESM
NTGTLASLQGRTRRAKGKNKHSKRALLVCQ
Nucleotide sequence for HDUB8.3
Atggaggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggccagatgcagcctttgctgaaatccagc
Ggacttctctccctgagaagtcacaactctcaactgagacccgcgtcgac
ttctgcgatgatttggcgcctgtggcaagacagcttgctcccagggagaa
Gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgtgaacgcttcccagcagtgtctgacatac
Acaccgccccttgccaactacatgctgtcccgggagcactctcaaacatg
tcatcgtcacaagtgctgcatgctctgtaccatggaagctcacatcacat
Ggcccctccacattcctggccatgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagctgcccttgaatttctcatgtt
Cactgtggatgccatgaaaaaggcatgccttcccgggcacaagcaggtag
atcatcactccaaggacaccaccctcatccaccaaatatttggagggtac
Tggagatctcaaatcaagtgtctccactgccacggcatttcagacacttt
tggcccttacctggacatcgccctggatatccaggaagctcagagtgtca
Agcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat
gcctatcattgtggcaacaaaattgccaagaatgtgcaatatcctgagtg
Ccttgacatgcagccatacatgtctcagcagaacacaggacctcttgtct
atgtcctctatgctgtgctggtccacgccgggtggagttgtcacaacgga
Cattacttctcttatgtcaaagttcaagaaggccagtggtataaaatgga
tgatgccgaggtcactgcctctggcatcacctctgtcctgagtcaacagg
Cctatgtcctcttttacatccacaagagtgaatgggaaagacacagtgag
agtgtgtcaagaggcagggaaccaagagccctcggcgctgaagacacaga
Caggcgagcaacgcaaggagagctcaagagagactacccctgcctccagg
tacccgagttggacgagcacttggtggaaagagccactcaggaaagcacc
Ttagaccactggaaattcctccaagagcaaaacaaaacgaagcctgagtt
caacgtcagaaaacttgaaggtaccctgcctcccaacgtacttgtgattc
Atcaatcaaaatacaagtgtgggatgaaaaaccatcatcctgaacagcaa
agctccctgctaaacctctcttcgacgaacccgacagatcaggagtccat
Gaacactggcacactcgcttctctgcaagggaggaccaggagagccaaag
ggaagaacaaacactgcaagagggctctgcttgtgtgccagtga
HDUB8.3 deduced polypeptide sequence
MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSQLSTETRVD
FCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASQQCLTY
TPPLANYMLSREHSQTCHRHKCCMLCTMEAHITWPLHIPGHVIQPSQALA
AGFHRGKQEAALEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGY
WRSQIKCLHCHGISDTFGPYLDIALDIQEAQSVKQALEQLVKPEELNGEN
AYHCGNKIAKNVQYPECLDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNG
HYFSYVKVQEGQWYKMDDAEVTASGITSVLSQQAYVLFYIHKSEWERHSE
SVSRGREPRALGAEDTDRRATQGELKRDYPCLQVPELDEHLVERATQEST
LDHWKFLQEQNKTKPEFNVRKLEGTLPPNVLVIHQSKYKCGMKNHHPEQQ
SSLLNLSSTNPTDQESMNTGTLASLQGRTRRAKGKNKHCKRALLVCQ
Nucleotide sequence for HDUB8.5
Atggaggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggccagatgcagcttttgctgaaatccagc
Ggacttctctccctgagaagtcaccactctcatctgaggcccgtgtcgac
ctctgtgatgatttggctcctgtggcaagacagcttgctcccaggaagaa
Gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgagaacgcttccctgcagtgcctgacatac
Acaccgccccttgccaactacatgctgtcccgggagcactctcaaacatg
tcagcgtcccaagtgctgcatgctctgtactatgcaagctcacatcacat
Gggccctccacagtcctggtcatgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagatgcccatgaatttctcatgtt
Cactgtggatgccatgaaaaaggcatgccttcccggccacaagcaggtag
atcatcactctaaggacaccaccctcatccaccaaatatttggaggctgc
Tggagatctcaaatcaagtgtctccactgccacgggatttcagacacttt
tgacccttacctggacatcgccctggatatccaggcagctcagagtgtca
Agcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat
gcctatcattgcggtctttgtcttcagagggcgccagtctccaagacgtt
Aactttacacacttttgccaaggaacgcatacttgaaacgcagagaccat
gggtggtcacacgccacaaactagccaagagtgtgcaatatgctgagagc
Cttgacatgcagccatacatgtctcagcagaacacaggacctcttgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacgatggac
Attacttctcttatgtcaaagctcaagaaggccagtggtataaaatggat
gatgccaaggtcactgcctgtagcatcacttctgtcctgagtcaacaggc
Ctatgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagccctcggcgctgaagacacagac
Aggcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggc
acccgagttggacgagcgcttggtggaaagagccactcaggaaagcacct
Tagaccactggagattcccccaagagcaaaacaaaacgaagcctgagttc
aacgtcagaaaagtcgaaggtaccctgcctcccaacgtacttgtgattca
Tcaatcgaaatacaagtgtgggatgaaaaaccatcatcctgaacagcaaa
gctccctgctaaacctctcttcgacgacccggacagatcaggagtccgtg
aacactggcaccctcgcttctctgcaagggaggaccaggagatccaaagg
gaagaacaaacacagcaagagggctctgcttgtgtgccagtga
HDUB8.5 deduced polypeptide sequence
MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSEARVD
LCDDLAPVARQLAPRKKLPLSSRRPAAVGAGLQNMGNTCYENASLQCLTY
TPPLANYMLSREHSQTCQRPKCCMLCTMQAHITWALHSPGHVIQPSQALA
AGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGC
WRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVKQALEQLVKPEELNGEN
AYHCGLCLQRAPVSKTLTLHTFAKERILETQRPWVVTRHKLAKSVQYAES
LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEGQWYKMD
DAKVTACSITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTD
RRATQGELKRDHPCLQAPELDERLVERATQESTLDHWRFPQEQNKTKPEF
NVRKVEGTLPPNVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTRTDQESV
NTGTLASLQGRTRRSKGKNKHSKRALLVCQ
Nucleotide sequence for HDUB8.6
Atggaagacgactcactctatttgggaggtgactggcagttcaatcactt
ttcaaaactcacatcttctcggctagatgcagcttttgctgaaatccagc
Ggacttctctctctgaaaagtcaccactctcatctgagacccgtttcgac
ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaa
Gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
agataggaaataccttctatgtgaacgtttccctgcagtgcctgacatac
Acactgccgctttccaactacatgctgtcccgggaggactctcaaacgtg
tcatcttcacaagtgctgcatgttctgtactatgcaagctcacatcacat
Gggccctctaccgtcctggccatgtcatccagccctcacaggtattggct
gctggcttccatagaggtgagcaggaggatgcccatgaatttctcatgtt
Tactgtggatgccatgaaaaaggcatgccttcccgggcacaagcagctag
atcatcactccaaggacaccaccctcatccaccaaatatttggagcgtat
Tggagatctcaaatcaagtatctccactgccacggcatttcagacacctt
tgacccttacctggacatcgccctggatatccaggcagctcagagtgtca
Agcaagctttggaacagttggtgaagcccaaagaactcaatggagagaat
gcctatcattgtggtctttgtctccagaaggcgcctgcctccaagacgtt
Aactttacccacttctgccaaggtcctcattcttgtattgaagagattct
ccgatgtcacaggcaacaaacttgccaagaatgtgcaatatcctaagtgc
Cgtgacatgcagccatacatgtctcagcagaacacaggacctcttgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacaacggac
Attacttctcttatgtcaaagctcaagaaggccagtggtataaaatggat
gatgccgaggtcactgcctctggcatcacctctgtcctgagtcaacaggc
Ctatgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagcccttggtgctgaagacacagac
Aggccagcaacgcaaggagagctcaagagagaccacccttgcctccaggt
acccgagttggacgagcacttggtggaaagagccactcaggaaagcacct
Tagaccactggaaattcccccaaaagcaaaacaaaacgaagcctgagttc
aacgtcagaaaagttgaaggtaccctgcctcccaacgtacttgtgattca
Tcaatcaaaatacaagtgtggtatgaaaaaccatcatcctgaacagcaaa
gctccctgctaaacctctcttcgacgaaaccgacagatcaggagtccatg
Aacactggcacactcgcttctctgcaagggagcaccaggagatccaaagg
gaataacaaacacagcaagagatctctgcttgtgtgccagtga
HDUB8.6 deduced polypeptide sequence
MEDDSLYLGGDWQFNHFSKLTSSRLDAAFAEIQRTSLSEKSPLSSETRFD
LCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQKIGNTFYVNVSLQCLTY
TLPLSNYMLSREDSQTCHLHKCCMFCTMQAHITWALYRPGHVIQPSQVLA
AGFHRGEQEDAHEFLMFTVDAMKKACLPGHKQLDHHSKDTTLIHQIFGAY
WRSQIKYLHCHGISDTFDPYLDIALDIQAAQSVKQALEQLVKPKELNGEN
AYHCGLCLQKAPASKTLTLPTSAKVLIIKLKRFSDVTGNKLAKNVQYPKC
RDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMD
DAEVTASGITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTD
RPATQGELKRDHPCLQVPELDEHLVERATQESTLDHWKFPQKQNKTKPEF
NVRKVEGTLPPNVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTKPTDQESM
NTGTLASLQGSTRRSKGNNKHSKRSLLVCQ
Nucleotide sequence for HDUB8.7
Atggaggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggccagatgcagcttttgctgaaatccagc
Ggacttctctccctgagaagtcaccactctcatctgaggcccgtgtcgac
ctctgtgatgatttggctcctgtggcaagacagcttgctcccaggaagaa
Gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgagaacgcttccctgcagtgcctgacatac
Acaccgccccttgccaactacatgctgtcccgggagcactctcaaacatg
tcagcgtcccaagtgctgcatgctctgtactatgcaagctcacatcacat
Gggccctccacagtcctggtcatgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagatgcccatgaatttctcatgtt
Cactgtggatgccatgaaaaaggcatgccttcccggccacaagcaggtag
atcatcactctaaggacaccaccctcatccaccaaatatttggaggctgc
Tggagatctcaaatcaagtgtctccactgccacgggatttcagacacttt
tgacccttacctggacatcgccctggatatccaggcagctcagagtgtca
Agcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat
gcctatcattgcggtctttgtctccagagggcgccagcctccaagacgtt
Aactttacacacttctgccaaggtcctcatccttgtcttgaagagattct
ccgatgtcacaggcaacaaacttgccaagaatgtgcaatatcctgagtgc
Cttgacatgcagccatacatgtctcagcagaacacaggacctcttgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacgatggac
Attacttctcttatgtcaaagctcaagaaggccagtggtataaaatggat
gatgccaaggtcactgcctgtagcatcacttctgtcctgagtcaacaggc
Ctatgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagccctcggcgctgaagacacagac
Aggcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggc
acccgagttggacgagcgcttggtggaaagagccactcaggaaagcacct
Tagaccactggagattcccccaagagcaaaacaaaacgaagcctgagttc
aacgtcagaaaagtcgaaggtaccctgcctcccaacgtacttgtgattca
Tcaatcgaaatacaagtgtgggatgaaaaaccatcatcctgaacagcaaa
gctccctgctaaacctctcttcgacgacccggacagatcaggagtccgtg
aacactggcaccctcgcttctctgcaagggaggaccaggagatccaaagg
gaagaacaaacacagcaagagggctctgcttgtgtgccagtga
HDUB8.7 deduced polypeptide sequence
MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSEARVD
LCDDLAPVARQLAPRKKLPLSSRRPAAVGAGLQNMGNTCYENASLQCLTY
TPPLANYMLSREHSQTCQRPKCCMLCTMQAHITWALHSPGHVIQPSQALA
AGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGC
WRSQIKCLHCHGISDTVDPYLDIALDIQAAQSVKQALEQLVKPEELNGEN
AYHCGLCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKLAKNVQYPEC
LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEGQWYKMD
DAKVTACSITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTD
RRATQGELKRDHPCLQAPELDERLVERATQESTLDHWRFPQEQNKTKPEF
NVRKVEGTLPPNVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTRTDQESV
NTGTLASLQGRTRRSKGKNKHSKRALLVCQ
Nucleotide sequence for HDUB8.8
Atggaagacgactcactctatttgggaggtgactggcagttcaatcactt
ttcaaaactcacatcttctcggctagatgcagcttttgctgaaatccagc
Ggacttctctctctgaaaagtcaccactctcatctgagacccgtttcgac
ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaa
Gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
agataggaaataccttctatgtgaacgtttccctgcagtgcctgacatac
Acactgccgctttccaactacatgctgtcccgggaggactctcaaacgtg
tcatcttcacaagtgctgcatgttctgtactatgcaagctcacatcacat
Gggccctctaccgtcctggccatgtcatccagccctcacaggtattggct
gctggcttccatagaggtgagcaggaggatgcccatgaatttctcatgtt
Tactgtggatgccatgaaaaaggcatgccttcccgggcacaagcagctag
atcatcactccaaggacaccaccctcatccaccaaatatttggagcgtat
Tggagatctcaaatcaagtatctccactgccacggcatttcagacacctt
tgacccttacctggacatcgccctggatatccaggcagctcagagtgtca
Agcaagctttggaacagttggtgaagcccaaagaactcaatggagagaat
gcctatcattgtggtctttgtctccagaaggcgcctgcctccaagacgtt
Aactttacccacttctgccaaggtcctcattcttgtattgaagagattct
ccgatgtcacaggcaacaaacttgccaagaatgtgcaatatcctaagtgc
Cgtgacatgcagccatacatgtctcagcagaacacaggacctcttgtcta
tgtcctctatgctgtgctggtccacgctgggtggagttgtcacaacggac
Attacttctcttatgtcaaagctcaagaaggccagtggtataaaatggat
gatgccgaggtcactgcctctggcatcacctctgtcctgagtcaacaggc
Ctatgtcctcttttacatccagaagagtgaatgggaaagacacagtgaga
gtgtgtcaagaggcagggaaccaagagcccttggtgctgaagacacagac
Aggccagcaacgcaaggagagctcaagagagaccacccttgcctccaggt
acccgagttggacgagcacttggtggaaagagccactcaggaaagcacct
Tagaccactggaaattcccccaaaagcaaaacaaaacgaagcctgagttc
aacgtcagaaaagttgaaggtaccctgcctcccaacgtacttgtgattca
Tcaatcaaaatacaagtgtggtatgaaaaaccatcatcctgaacagcaaa
gctccgtgctaaacctctcttcgacgaaaccgacagatcaggagtccatg
aacactggcacactcgcttctctgcaagggagcaccaggagatccaaagg
gaataacaaacacagcaagagatctctgcttgtgtgccagtga
HDUB8.8 deduced polypeptide sequence
MEDDSLYLGGDWQFNHFSKLTSSRLDAAFAEIQRTSLSEKSPLSSETRFD
LCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQKIGNTFYVNVSLQCLTY
TLPLSNYMLSREDSQTCHLHKCCMFCTMQAHITWALYRPGHVIQPSQVLA
AGFHRGEQEDAHEFLNFTVDAMKKACLPGHKQLDHHSKDTThIHQIFGAY
WRSQIKYLHCHGISDTFDPYLDIALDIQAAQSVKQALEQLVKPKELNGEN
AYHCGLCLQKAPASKTLTLPTSAKVLILVLKRFSDVTGNKLAKNVQYPKC
RDMQPYMSQQNTGPLVYTLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMD
DAEVTASGITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTD
RPATQGELKRDHPCLQVPELDEHLVERATQESTLDHWKFPQKQNKTKPEF
NVRKVEGTLPPNVLVIHQSKYKCGMKNHHPEQQSSVLNLSSTKPTDQESM
NTGTLASLQGSTRRSKGNNKHSKRSLLVCQ
Nucleotide sequence for HDUB8.11
Atggaggacgactcactctacttgggaggtgagtggcagttcaaccactt
ttcaaaactcacatcttctcggccagatgcagcctttgctgaaatccagc
Ggacttctctccctgagaagtcacaactctcaactgagacccgcgtcgac
ttctgcgatgatttggccgctgtggcaagacagctcgctcccagggagaa
Gcttcctctgagtagcaggagacctgctgcggtgggggctgggctccaga
atatgggaaatacctgctacgtgaacgcttcccagcagtgtctgacatac
Ataccgccccttgccaactacatgctgtcccgggagcactctcaaacatg
tcatcgtcacaagtgctgcatgctctgtaccatggaagctcacatcacat
Ggcccctccacattcctggccatgtcatccagccctcacaggcattggct
gctggcttccatagaggcaagcaggaagctgcccttgaatttctcatgtt
Cactgtggatgccatgaaaaaggcatgccttcccgggcacaagcagatcc
tcatcctcgtatggaagagattctccgatgtcacaggcaacaaaattgcc
Aagaatgtgcaatatcctgagtgccttgacatgcagccatacatgtctca
gcagaacacaggacctcttgtctatgtcctctatgctgtgctggtccacg
Ccgggtggagttgtcacaacggacattacttctcttatgtcaaagttcaa
gaaggccagtggtataaaatggatgatgccgagaagagtgaatgggaaag
Acacagtgagagtgtgtcaagaggcagggaaccaagagccctcggcgctg
aagacacagacaggcgagcaacgcaaggagagctcaagagagactacccc
Tgcctccaggtacccgagttggacgagcacttggtggaaagagccactca
ggaaagcaccttagaccactggaaattcctccaagagcaaaacaaaacga
Agcctgagttcaacgtcagaaaacttgaaggtaccctgcctcccaacgta
cttgtgattcatcaatcaaaatacaagtgtgggatgaaaaaccatcatcc
Tgaacagcaaagctccctgctaaacctctcttcgacgaacccgacagatc
aggagtccatgaacactggcacactcgcttctctgcaagggaggaccagg
agatccaaagggaagaacaaacactgcaagagggctctgcttgtgtgcca
gtga
HDUB8.11 deduced polypeptide sequence
MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSQLSTETRVD
FCDDLAAVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASQQCLTY
IPPLANYMLSREHSQTCHRHKCCMLCTNEAHITWPLHIPGHVIQPSQALA
AGFHRGKQEAALEFLMFTVDAMKKACLPGHKQILILVWKRFSDVTGNKIA
KNVQYPECLDMQPYNSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKVQ
EGQWYKMDDAEKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDYP
CLQVPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKLEGTLPPNV
LVIHQSKYKCGMKNHHPEQQSSLLNLSSTNPTDQESMNTGTLASLQGRTR
RSKGKNKHCKRALLVCQ
Nucleotide sequence for HDUB4.4 sequence
atggaggagg actcactcta cttgggtggt gagtggcagt
tcaaccactt ttcaaaactc acatcttctc ggctcgatgc
agcttttgct gaaatccagc ggacttctct ccctgagaag
tcaccactct catgtgagac ccgtgtcgac ctctgtgatg
atttggttcc tgaggcaaga cagcttgctc ccagggagaa
gcttcctctg agtagcagga gacctgctgc ggtgggggct
gggctccaga atatgggaaa tacctgctac gtgaacgctt
ccttgcagtg cctgacatac acaccgcccc ttgccaacta
catgctgtcc cgggagcact ctcaaacgtg tcatcgtcac
aagggctgca tgctctgtac tatgcaagct cacatcacac
gggccctcca caatcctggc cacgtcatcc agccctcaca
ggcattggct gctggcttcc atagaggcaa gcaggaagat
gcccatgaat ttctcatgtt cactgtggat gccatgaaaa
aggcatgcct tcccgggcac aagcaggtag atcatccctc
taaggacacc accctcatcc accaaatatt tggaggctac
tggagatctc aaatcaagtg tctccactgc cacggcattt
cagacacttt tgacccttac ctggacatcg ccctggatat
ccaggcagct cagagtgtcc agcaagcttt ggaacagttg
gtgaagcccg aagaactcaa tggagagaat gcctatcatt
gtggtgtttg tctccagagg gcgccggcct ccaagacgtt
aactttacac acttctgcca aggtcctcat ccttgtattg
aagagattct ccgatgtcac aggcaacaag attgccaaga
atgtgcaata tcctgagtgc cttgacatgc agccatacat
gtctcagcag aacacaggac ctcttgtcta tgtcctctat
gctgtgctgg tccatgctgg gtggagttgt cacaacggac
attacttctc ttatgtcaaa gctcaagaag gccagtggta
taaaatggat gatgccgagg tcaccgcctc ttagcatcac
atctgtcctg agtcaacagg cctacgtcct cttttacatc
cagaagagtg aatgggaaag acacagtgag agtgtgtcaa
gaggcaggga accaagagcc cttggcgcag aagacacaga
caggcgagta acgcaaggag agctcaagag agaccacccc
tgactccagg cccccgagtt ggacgagcac ttggtggaaa
gagccactca ggaaagcacc ttagaccact ggaaattcct
tcaagagcaa aacaaaacga agcctgagtt caacgtcaga
aaagtcgaag gtaccctgcc tcccgacgta cttgtgattc
atcaatcaaa atacaagtgt gggatgaaga accatcatcc
tgaacagcaa agctccctgc taaacctctc ttcgacgacc
ccgacacatc agcagtccat gaacaatggc acactcgctt
ccctgcgagg gagggccagg agatccaaag ggaagaacaa
acacagcaag agggctctgc ttgtgtgcca gtga
hDUB4.4 Deduced polypeptide sequence
MEEDSLYLGG EWQFNHFSKL TSSRLDAAFA EIQRTSLPEK
SPLSCETRVD LCDDLVPEAR QLAPREKLPL SSRRPAAVGA
GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH
KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED
AHEFLMFTVD ANKKACLPGH KQVDHPSKDT TLIHQIFGGY
WRSQIKCLHC HGISDTFDPY LDIALDIQAA QSVQQALEQL
VKPEELNGEN AYHCGVCLQR APASKTLTLH TSAKVLILVL
KRFSDVTGNK IAKNVQYPEC LDMQPYNSQQ NTGPLVYVLY
AVLVHAGWSC HNGHYFSYVK AQEGQWYKMD DAEVTAS
Nucleotide sequence for hDUB4.9
atggaggacg actcactcta cttgggaggt gagtggcagt
tcaaccactt ttcaaaactc acatctcctc ggcccgatgc
agcttttgct gaaatccagc ggacttctct ccctgagaag
tcaccactct catgtgagac ccgtgtcgac ctctgtgatt
atttggctcc tgtggcaaga cagcttgctc ccagggagaa
gcttcctctg agtagcagga gacctgctgc ggtgggggct
gggctccaga atatgggaaa tacctgctac gtgaacgctt
ccttgcagtg cctgacatac acaccgcccc ttgccaacta
catgctgtcc cgggagcact ctcaaacgtg tcatcgtcac
aagggctgca tgctctgtac tatgcaagct cacatcacac
gggccctcca caatcctggc cacgtcatcc agccctcaca
ggcattggct gctggcttcc atagaggcaa gcaggaagat
gcccatgaat ttctcatgtt cactgtggat gccatgaaaa
aggcatgcct tcccgggcac aagcaggtgg atcatcactc
taaggacacc accctcatcc accaaatatt tggaggctac
tggagatctc aaatcaagtg tctccactgc cacggcattt
cagacacttt tgacccttac ctggacatcg ccctggatat
ccaggcagct cagagtgtcc agcaagcttt ggaacagttg
gtgaagcccg aagaactcaa tggagagaat gcctatcatt
gtggtgtttg tctccagagg gcgccggcct ccaagacgtt
aactttacac acctctgcca aggtcctcat ccttgtattg
aagagattct ccgatgtcac aggcaacaag attgccaaga
atgtgcaata tcctgagtgc cttgacatgc agccatacat
gtctcagcag aacacaggac ctcttgtcta tgtcctctat
gctgtgctgg tccacgctgg gtggagttgt cacaacggac
attacttctc ttatgtcaaa gctcaagaag gccagtggta
taaaattgat gatgccgagg tcaccgcctc tagcatcact
tctgtcctga ctcaacaggc ctacgtcctc ttttacatcc
agaagagtga atgggaaaga cacagtgaga gtgtgtcaag
aggcagggaa ccaagagccc ttggctctga agactaa
hDUB4.9 deduced polypeptide sequence
MEDDSLYLGG EWQFNHFSKL TSPRPDAAFA EIQRTSLPEK
SPLSCETRVD LCDYLAPVAR QLAPREKLPL SSRRPAAVGA
GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH
KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED
AHEFLMFTVD AMKKACLPGH KQVDHHSKDT TLIHQIFGGY
WRSQIKCLHC HGISDTFDPY LDIALDIQAA QSVQQALEQL
VKPEELNGEN AYHCGVCLQR APASKTLTLH TSAKVLILVL
KRFSDVTGNK IAKNVQYPEC LDMQPYNSQQ NTGPLVYVLY
AVLVHAGWSC HNGHYFSYVK AQEGQWYKID DAEVTASSIT
SVLTQQAYVL FYIQKSEWER HSESVSRGRE PRALGSED
Nucleotide sequence for hDUB8.2 sequence
atgcggccag agagcccgtc atttgaagac tcggaagaga
tagcgtcttt ctgcaacctg cggtcccagc cgaaaaacct
tgtgatcctt gttccgggcg acatggagga cgactcactc
tacttgggag gtgagtggca gttcaaccac ttttcaaaac
tcacatcttc tcggccagat gcagcttttg ctgaaatcca
gcggacttct ctctctgaga agtcatcact ctcatctgag
acccgcgtcg acctctgtga tgatttggct cctgtggcaa
gacagctcgc tcccagggag aagcttcctc tgagtagcag
gagacctgct gcggtggggg ctgggctcca gaatatggga
aatacctgct acgtgaacgc ttccctgcag tgcctgacat
acacaccgcc ccttgccaac tacatgctgt cccgggagca
ctctcaaacg tgtcatcgtc acaagtgctg catgctctgt
actatgcaag ctcacatcac atggcccctc cacagtcctg
gccatgtcat ccagccctca caggtgttgg ctgctggctt
ccatagaggc gagcaggaag atgcccatga atttctcatg
ttcactgtgg atgccatgaa aaaggcattc cttcccgggc
acaagcattt agataatcac tctaaggaca ccaccctcat
ccaccaaata tttggagggt actggagatc tcacatcaac
tgtttccact gccacgggat ttcagacacc tttgaccctt
acctggacat cgccctggat atccaggcag ctcagagtgt
caagcaagct ttgtaacagt tggtgaagcc cgaagaactc
aatggataaa atgcctatca ttgtggtctt tgtctccaga
aggcgcctgc ctccaggacg ttaactttac acacttctgc
caaggtcctc atccttgtat tgaagagatt ctctgaggtc
acaggcaaca aacttgccaa gaatgtgcaa tatcctgagt
gccttgacat gcagccatac atgtctcagc agaacacagg
acctcttgtc tatgtcctct atgctgtgct ggtccacgct
gggtggagtt gtcacaacgg acattactta tcttatgtca
aactcaagaa ggccattggt ataaaatgga tgatgccgag
gtcactgcct ccggtatcac ttctgtcctg agtcaacagg
cctatgtcct cttttacatc cagaagaatg aatttggaag
acccagttac agtgtgtcca taggcaggga accaagagct
ctttgcgtga aggcaagtga attgtgtgtg aaataaaatg
tcatgaataa atcttgcagt ggagtattta tttgtctcac
tttgtaatca gtgaatgagc tttaaccaat atcaatgcct
agtgcctacc ccccagagat aagaacttcc actctcttat
gtgtaaccat ggcctctgga ttgcttatga ctctgaagat
aattctcctt tcccccaacg tttcagaatc acttcaggtg
gtggtaacag ataacacatc agtccctttc tctctctttt
ctcttcactc aggaaaactc tcactgagac aaaggaaaat
cctatggttt actggggagg aagaattccc tcaggagtga
aattggtggc tccttcctcc ctgtcaagtc tcttcctcag
gattgcccct ttgtctcttc aggact
hDUB8.2 dedeuced polypeptide sequence
MRPESPSFED SEEIASFCNL RSQPKNLVIL VPGDMEDDSL
YLGGEWQFNH FSKLTSSRPD AAFAEIQRTS LSEKSSLSSE
TRVDLCDDLA PVARQLAPRE KLPLSSRRPA AVGAGLQNMG
NTCYVNASLQ CLTYTPPLAN YMLSREHSQT CHRHKCCMLC
TMQAHITWPL HSPGHVIQPS QVLAAGFHRG EQEDAHEFLM
FTVDANKKAF LPGHKHLDNH SKDTTLIHQI FGGYWRSHIN
CFHCHGISDT FDPYLDTALD IQAAQSVKQA L
Nucleotide sequence for hDUB8.9
atggaggaag actcactcta cttgggaggt gagtggcagt
tcaaccactt ttcaaaactc acatcttctc agccagatgc
agcttttcct gaaatccagc ggacttctct ccctgagaag
tcaccactct catcggagac ccgtgtcgac ctctgtgacg
atttggctcc tgtgacaaga cagcttgctc ccagggagaa
gcttcctccg agtagcagga gacctgctgc ggtgggagct
ggtctccaga atatgggaaa tacctgccac ttgaatgctt
ccctgcagtg cctgacatac acaccgcccc ttgccaacta
catgctgtcc tgggagctct ctcaaatgtg tcatcgtccc
aagtgctgca tgctctgtat tatggaagct cacagcacac
gggcacctcc accgtcctgg ccatgtcatc cagccctcac
aggcattggc tgctgacttc catagagaca agcaggaaga
tgcccatgaa tttctcatat tcactgtgga tgccattaga
aaggcatgcc ttcccgggca caagcagcta gatcatcact
gcaaggacac catcctcatc caccaaatat ttggagggta
ctagagatct caaatcaagt gtctctactt ccacggcatt
tcagacacct tcgaccctta cctggatatc gccctggata
tccaggcagc tcagagtgtc aagcaagctt tggaacagtt
ggtgaagccc gaagaactca atggagagaa tgcctatcat
tgtggtcttt gtctccagaa ggcgcctgcc gccaagacgt
taactttacc cacttctgcc aaggtcctca tccttgtctt
gaagagattc tccgatgtca caggcaacaa acttgccaag
aatctgcaat atcctgagtg cgttgacatg cagccataca
tgtctcagca gaacacagga cctcttttct atgtcctcta
tgctgttctc gtcatcaccg ggtggagttg tcacaacgga
cattacttct cttgtgtcaa actcaagaag gccagtggta
taaaatggat gatgccgagg tcactgcctc tggtatcact
tctccttaga gtcaacaggc ctatgtcctc ttttacatcc
agaagaatga atttggaaga cccagttaca gggtgtccgc
aggcagagaa ccaagagctc tttgtgctga agacaattga
attgtggtga aataatatgt catgaataaa tcttgcagca
gatttatttg tctcactttg taatcagtga atgagcttta
acgaatatca atgcctagtg cctacccccc agagataaga
acttccagtt tctcatgtgt aatcatggca tctggattgc
tcatgattct gaagataatt ctcctgtccc ccaaagtttc
agaatcactt caggtggtag aaacagataa cacatcagtc
cctttctctc tcttttctct tca
hDUB8.9 Deduced polypeptide sequence
MEDDSLYLGG EWQFNHFSKL TSSRPDAAFA EIQRTSLSEK
SSLSSETRVD LCDDLAPVAR QLAPREKLPL SSRRPAAVGA
GLQNMGNTCY VNASLQCLTY TPPLANYNLS REHSQTCHRH
KCCMLCTMQA HITWPLHSPG HVIQPSQVLA AGFHRGEQED
AHEFLNFTVD AMKKAFLPGH KHLDNHSKDT TLIHQIFGGY
WRSHINCFHC HGISDTFDPY LDIALDIQAA QSVKQALEQL
VKPEELNG
Nucleotide sequence for hDUB8.10
atggaggacg actcactcta cttgggaggt gagtggcagt
tcaaccactt ttcaaaactc acatcttctc ggccagatgc
agcttttgct gaaatccagc ggacttctct ctctgagaag
tcatcactct catctgagac ccgcgtcgac ctctgtgatg
atttggctcc tgtggcaaga cagctcgctc ccagggagaa
gcttcctctg agtagcagga gacctgctgc ggtgggggct
gggctccaga atatgggaaa tacctgctac gtgaacgctt
ccctgcagtg cctgacatac acaccgcccc ttgccaacta
catgctgtcc cgggagcact ctcaaacgtg tcatcgtcac
aagtgctgca tgctctgtac tatgcaagct cacatcacat
ggcccctcca cagtcctggc catgtcatcc agccttcaca
ggtgttggct gctggcttcc atagaggcga gcaggaagat
gcccatgaat ttctcatgtt cactgtggat gccatgaaaa
aagcattcct tcccgggcac aagcatttag ataatcactc
taaggacacc accctcatcc accaaatatt tggagggtac
tggagatctc acatcaactg tttccactgc catgggattt
cagacacctt tgacccttac ctggacatcg ccctggatat
ccaggcagct cagagtgtca agcaagcttt ggaacagttg
gtgaagcccg aagaactcaa tggataaaat gcctatcatt
gtggtctttg tctccagaag gcgcctacct ccaggacgtt
aactttacac acttctgcca aggtcctcat ccttgtattg
aagagattct ctgatgtcac aggcaacaaa cttgccaaga
atgtgcaata tcctgagtgc cttgacatgc agccatacat
gtctcagcag aacacaggac ctcttgtcta tgtcctctat
gctgtgctgg tccacgctgg gtggagttgt cacaacggac
attacttatc ttatgtcaaa ctcaagaagg ccattggtat
aaaatggatg atgccgaggt cactgcctcc ggtatcactt
ctgtcctgag tcaacaggcc tatgtcctct tttacatcca
gaagaatgaa tttggaagac ccagttacag tgtgtccata
ggcagggaac cgagagctct ttgcgtgaag gcaagtgaat
tgtgtgtgaa ataaaatgtc atgaataaat cttgcagtgg
agtatttatt tgtctcactt tgtaatcagt gaatgagctt
taaccaatat caatgcctag tgcctacccc ccagagataa
gaacttccac tctcttatgt gtaaccatgg cctctggatt
gcttatgact ctgaagataa ttctcctttc ccccaacgtt
tcagaatcac ttcaggtggt ggtaacagat aacacatcag
tccctttctc tctcttttct cttcactcag gaaaactctc
actgagacaa aggaaaatcc tatggtttac tggggaggaa
gaattccctc aggagtgaaa ttggtggctc cttcctccct
gtcaagtctc ttcctcagga ttgccccttt gtctcttcag
gactctgctc atcaggcccg agatgccccc tggttgtgca
tacctggcct gtgaagaaat a
hDUB8.10 Deduced polypeptide sequence
MEEDSLYLGG EWQFNHFSKL TSSQPDAAFP EIQRTSLPEK
SPLSSETRVD LCDDLAPVTR QLAPREKLPP SSRRPAAVGA
GLQNMGNTCH LNASLQCLTY TPPLANYMLS WELSQMCHRP
KCCMLCIMEA HSTRAPPPSW PCHPALTGILG C

TABLE 24
Deduced Amino acid alignment of hDUB4.10 (Seq ID NO.22) and
hDUB4.11 (Seq ID NO.24).
hDUB4.10 MCIRTGSPCDVCENYSVMSMTGRQLIDWAPLKIGYEHSSTPMPREHVHFRQHYNFGTKCA  60
hDUB4.11 MCIRTGSPCDVCENYSVMSMTGRQLIDWAPLKIGYEHSSTPMPRT-LYIRHRK-------  52
         ********************************************  :::*::
hDUB4.10 NCNSTIQCVTGNGGNIADPERSMRESRICTAYFGLFPLKQGPVLKMVISLGQRINRLNVE 120
hDUB4.11 ---------PSDGAHLAHEK-------------------------------------TRE  66
         ..:*.::*. :                                     . *
hDUB4.10 RLSLEGKKIRCAKYYTSLTILRSESALSTSCPSVAERMMAAAKRIASFCNLRSQQKNLVI 180
hDUB4.11 RNGAKGKKIRCAKYYTSLTILRSESALSSSCPSVAERMMAAAK----------------- 109
         * . :***********************:**************
hDUB4.10 LVPVDMEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDL 240
hDUB4.11 ---IDMEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLS--------YDL 158
         :********************************************* **         **
hDUB4.10 APVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQ 300
hDUB4.11 APVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQ 218
         **********************************************.*************
hDUB4.10 TCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKA 360
hDUB4.11 TCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKA 278
         ************************************************************
hDUB4.10 CLPGHKQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQ 420
hDUB4.11 CLPRHKQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQ 338
         *** ********************************************************
hDUB4.10 ALEQLVKPEELNGENAYHSGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNV 480
hDUB4.11 ALEQLVKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNV 398
         ******************.*****************************************
hDUB4.10 QYPECLDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVT 540
hDUB4.11 QYPECLDMQPYMSQQNTGPLVYVLYAVLVHAEWSCHNGHYFSYVKAQEGQWYKMDDAEVT 458
         ******************************* ****************************
hDUB4.10 AASITSALSQQAYVLFYIQKSEWERHSESVSRGREPRALGTEDTDRRATQGELKRDHPCL 600
hDUB4.11 AASITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCL 518
         ******.*********************************:*******************
hDUB4.10 QAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGM 660
hDUB4.11 QAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVKGTLPPDVLVIHQSKYKCGM 578
         ****************************************:*******************
hDUB4.10 KNHHPEQQSSLLNLSSSTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ      715
hDUB4.11 KNHHPEQQSSLLNLSSSTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVC-      632
         ******************************************************

TABLE 25
Nucleotide sequence alignment of hDUB4.5 (Seq ID NO.13), hDUB4.8
(Seq ID NO.19) and hDUB8.2 (Seq ID NO.43).
hDUB4.5 ATGCG-CCAGAGACCTCGTCATTTGAAGACTCTCTCGGAAGGGATAGCGTCTTTCTGCAA   59
hDUB4.8 ATGCG-CCAGAGAGCTCGTCATTTGAAGACTCTCTCGGAAGGGATAGCGTCTTGCTGCAA   59
hDUB8.2 ATGCGGCCAGAGAGCCCGTCATTTGAAGA----CTCGGAAGAGATAGCGTCTTTCTGCAA   56
        ***** ********* *************    ******** *********** ******
hDUB4.5 CCTGCGGTCCCAGCAGAAAAACCTTGTGATCCTTGTTCCAGTCGACATGGAGGAAGACTC  119
hDUB4.8 ACTGCGGTCCCAGCAGAAAAACCTTGTGATCCTTGTTCCAGTCGACATGGAGGACGACTC  119
hDUB8.2 CCTGCGGTCCCAGCCGAAAAACCTTGTGATCCTTGTTCCGGGCGACATGGAGGACGACTC  116
        ************* ************************ * ************ *****
hDUB4.5 ACTCTACTTGGGAGGTGAGTGGCAGTTCAACCACTTTTCAAAACTCACATCTTCTCGGCC  179
hDUB4.8 ACTCTACTTGGGAGGTGAGTGGCAGTTCAACCACTTTTCAAAACTCACATCTTCTCGGCC  179
hDUB8.2 ACTCTACTTGGGAGGTGAGTGGCAGTTCAACCACTTTTCAAAACTCACATCTTCTCGGCC  176
        ************************************************************
hDUB4.5 CGATGCAGCTTTTGCTGAAATCCAGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATG  239
hDUB4.8 CGATGCAGCTTTTGCTGAAATCCAGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATG  239
hDUB8.2 AGATGCAGCTTTTGCTGAAATCCAGCGGACTTCTCTCTCTGAGAAGTCATCACTCTCATC  236
        ************************************ *********** *********
hDUB4.5 TGAGACCCGTGTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG  299
hDUB4.8 TGAGACCCGTGTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG  299
hDUB8.2 TGAGACCCGCGTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTCGCTCCCAG  296
        ********* ***************************************** ********
hDUB4.5 GGAGAAGCTTCCTCTGAGTAACAGGAGACCTGCTGCGGTGGGGGCTGGGCTCCAGAATAT  359
hDUB4.8 GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGTGGGGGCTGGGCTCCAGAATAT  359
hDUB8.2 GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGTGGGGGCTGGGCTCCAGAATAT  356
        ******************** ***************************************
hDUB4.5 GGGAAATACCTGCTACGTGAACGCTTCCTTGCAGTGCCTGACATACACACCGCCCCTTGC  419
hDUB4.8 GGGAAATACCTGCTACGTGAACGCTTCCTTGCAGTGCCTGACATACACACCGCCCCTTGC  419
hDUB8.2 GGGAAATACCTGCTACGTGAACGCTTCCCTGCAGTGCCTGACATACACACCGCCCCTTGC  416
        **************************** *******************************
hDUB4.5 CAACTACATGCTGTCCCGGGAGCACTCTCAAACGTGTCATCGTCACAAGGGCTGCATGCT  479
hDUB4.8 CAACTACATGCTGTCCCGGGAGCACTCTCAAACGTGTCATCGTCACAAGGGCTGCATGCT  479
hDUB8.2 CAACTACATGCTGTCCCGGGAGCACTCTCAAACGTGTCATCGTCACAAGTGCTGCATGCT  476
        ************************************************* **********
hDUB4.5 CTGTACGATGCAAGCTCACATCACACGGGCCCTCCACAATCCTGGCCACGTCATCCAGCC  539
hDUB4.8 CTGTACGATGCAAGCTCACATCACACGGGCCCTCCACAATCCTGGCCACGTCATCCAGCC  539
hDUB8.2 CTGTACTATGCAAGCTCACATCACATGGCCCCTCCACAGTCCTGGCCATGTCATCCAGCC  536
        ****** ****************** ** ********* ********* ***********
hDUB4.5 CTCACAGGCATTGGCTGCTGGCTTCCATAGAGGCAAGCAGGAAGATGCCCATGAATTTCT  599
hDUB4.8 CTCACAGGCATTGGCTGCTGGCTTCCATAGAGGCAAGCAGGAAGATGCCCATGAATTTCT  599
hDUB8.2 CTCACAGGTGTTGGCTGCTGGCTTCCATAGAGGCGAGCAGGAAGATGCCCATGAATTTCT  596
        ******** ************************ *************************
hDUB4.5 CATGTTCACTGTGGATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGCAGGTGGATCA  659
hDUB4.8 CATGTTCACTGTGGATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGCAGGTAGATCA  659
hDUB8.2 CATGTTCACTGTGGATGCCATGAAAAAGGCATTCCTTCCCGGGCACAAGCATTTAGATAA  656
        ******************************** ******************  * *** *
hDUB4.5 TCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGAGGCTACTGGAGATCTCAAAT  719
hDUB4.8 TCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGAGGCTACTGGAGATCTCAAAT  719
hDUB8.2 TCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGAGGGTACTGGAGATCTCACAT  716
        ****************************************** ************** **
hDUB4.5 CAAGTGTCTCCACTGCCACGGCATTTCAGACACTTTTGACCCTTACCTGGACATCGCCCT  779
hDUB4.8 CAAGTGTCTCCACTGCCACGGCATTTCAGACACTTTTGACCCTTACCTGGACATCGCCCT  779
hDUB8.2 CAACTGTTTCCACTGCCACGGGATTTCAGACACCTTTGACCCTTACCTGGACATCGCCCT  776
        *** *** ************* *********** **************************
hDUB4.5 GGATATCCAGGCAGCTCAGAGTGTCCAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGA  839
hDUB4.8 GGATATCCAGGCAGCTCAGAGTGTCCAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGA  839
hDUB8.2 GGATATCCAGGCAGCTCAGAGTGTCAAGCAAGCTTTGTAACAGTTGGTGAAGCCCGAAGA  836
        ************************* *********** **********************
hDUB4.5 ACTCAATGGAGAGAATGCCTATCATTGTGGTGTTTGTCTCCAGAGGGCGCCGGCCTCCAA  899
hDUB4.8 ACTCAATGGAGAGAATGCCTATCATTGTGGTGTTTGTCTCCAGAGGGCGCCGGCCTCCAA  899
hDUB8.2 ACTCAATGGATAAAATGCCTATCATTGTGGTCTTTGTCTCCAGAAGGCGCCTGCCTCCAA  896
        ********** * ****************** ************ ****** *******
hDUB4.5 GACGTTAACTTTACACACCTCTGCCAAGGTCCTCATCCTTGTATTGAAGAGATTCTCCGA  959
hDUB4.8 GACGTTAACTTTACACACCTCTGCCAAGGTCCTCATCCTTGTATTGAAGAGATTCTCCGA  959
hDUB8.2 GACGTTAACTTTACACACTTCTGCCAAGGTCCTCATCCTTGTATTGAAGAGATTCTCTGA  959
        ****************** ************************************** **
hDUB4.5 TGTCACAGGCAACAAGATTGACAAGAATGTGCAATATCCTGAGTGCCTTGACATGAAGCT 1019
hDUB4.8 TGTGACAGGCAACAAGATTGCCAAGAATGTGCAATATCCTGAGTGCCTTGACATGCAGCC 1019
hDUB8.2 GGTCACAGGCAACAAACTTGCCAAGAATGTGCAATATCCTGAGTGCCTTGACATGCAGCC 1016
        ** ***********  *** ********************************** ***
hDUB4.5 ATACATGTCTCAGACGAACTCAGGACCTCTCGTCTATGTCCTCTATGCTGTGCTGGTCCA 1079
hDUB4.8 ATACATGTCTCAGCAGAACACAGGACCTCTTGTCTATGTCCTCTATGCTGTGCTGGTCCA 1079
hDUB8.2 ATACATGTCTCAGCAGAACACAGGACCTCTTGTCTATGTCCTCTATGCTGTGCTGGTCCA 1076
        *************  **** ********** *****************************
hDUB4.5 CGCTGGGTGGAGTTGTCACAACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCA 1139
hDUB4.8 CGCTGGGTGGAGTTGTCACAACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCA 1139
hDUB8.2 CGCTGGGTGGAGTTGTCACAACGGACATTACTTATCTTATGTCAAA-CTCAAGAAGGCCA 1135
        ********************************* ************ *************
hDUB4.5 GTGGTATAAAATGGATGATGCCGAGGTCACCGCCTCTAGCATCACTTCTGTCCTGAGTCA 1199
hDUB4.8 ATGGTATAAAATGGATGATGCCGAGGTCACCGCCGCTAGCATCACTTCTGTCCTGAGTCA 1199
hDUB8.2 TTGGTATAAAATGGATGATGCCGAGGTCACTGCCTCCGGTATCACTTCTGTCCTGAGTCA 1195
        ***************************** *** * * ********************
hDUB4.5 ACAGGCCTACGTCCTCTTTTACATCCAGAAGAGTGAATGGGAAAGACACAGTGAGAGTGT 1259
hDUB4.8 ACAGGCCTACGTCCTCTTTTACATCCAGAAGAGTGAATGGGAAAGACACAGTGAGAGTGT 1259
hDUB8.2 ACAGGCCTATGTCCTCTTTTACATCCAGAAGAATGAATTTGGAAGACCCAGTTACAGTGT 1259
        ********* ********************** *****  * ***** **** * *****
hDUB4.5 GTCAAGAGGCAGGGAAACCAAGAGCCCTTGGCGCAGAAGACACAGACAGGCGAGCAACGCA 1319
hDUB4.8 GTCAAGAGGCAGGGAAACCAAGAGCCCTTGGCGCAGAAGACACAGACAGGCGAGCAACGCA 1319
hDUB8.2 GTCCATAGGCAGGGAAACCAAGAGCTCTTTGCGTGAAGGCAAGTGAATTGTGTGTGAAATA 1315
        *** * ******************* *** ***   * *  *  **   * * *  *   *
hDUB4.5 AGGAGAGCTCAAGAGAGACCACCCCTGCCTCCAGGCCCCCGAGTTGGACGAGCACTTGGT 1379
hDUB4.8 AGGAGAGCTCAAGAGAGACCACCCCTGCCTCCAGGCCCCCGAGTTGGACGAGCACTTGGT 1379
hDUB8.2 AAATG---TCATGA---ATAAATCTTGCAGTGGAGTATTT-ATTTGTCTCACTTTGTAAT 1368
        *   *   *** **   *  *  * ***      *      * ***    *     *  *
hDUB4.5 GGAAAGAGCCACTCAGGAAAGCACCTTAGACCACTGGAAATTCCTTCAAGAGCAAAACAA 1439
hDUB4.8 GGAAAGAGCCACTCAGGAAAGCACCTTAGACCACTGGAAATTCCTTCAAGAGCAAAACAA 1439
hDUB8.2 CAGTGAATGAGCTTTAACCAATATCAATGCCTAGTGCCTACCCCCCAGAGATAAGAACTT 1428
        *    **      *  * *   * * * **   *  **    ***  * ***
hDUB4.5 AACGAAGCCTGAGTTCAACGTCAGAAAAGTCGAAGGTACCCTGCCTCCCGACGTACTTGT 1499
hDUB4.8 AACGAAGCCTGAGTTCAACGTCAGAAAAGTCGAAGGTACCCTGCCTCCCGACGTACTTGT 1499
hDUB8.2 CCACTCTCTTATGTGTAAC--CATGGCCTCTGGATTGCTTATGACTCTGAAGATAATTCT 1486
        * *  **  ***  **        * *       ** ***   *  ** ** *
hDUB4.5 GATTCATCAATCAAAATACAAGTGTGGGATGAAGAACCATCATCCTG-AACAGCAAAGCT 1558
hDUB4.8 GATTCATCAATCAAAATACAAGTGTGGGATGAAGAACCATCATCCTG-AACAGCAAAGCT 1558
hDUB8.2 CCTT--TCCCCCAACGTTTCAGAATCACTTCAGGTGGTGGTAACAGATAACACATCAGTC 1544
        **  **   ***  *   **  *    * * *       * *    ****    **
hDUB4.5 CCCTGCTAAACCTCTCTTCGACGACCCCGACACATCAGGAGTCCATGAACACTGGCACAC 1618
hDUB4.8 CCCTGCTAAACCTCTCTTCGACGACCCCGACACATCAGGAGTCCATGAACACTGGCACAC 1618
hDUB8.2 CCTTTCTCTCTCTTTTCTCTTCACTCAGGAAAACTCTCACTGAGACAAAGGAAAATCCTA 1604
        ** * **    ** *  **  *   *  ** *  **        *  **        *
hDUB4.5 TCGCTTCCCTGCGAGGGAGGGC-----CAGGAGATCCAAAGGGAAGAACAAACACAGCAA 1673
hDUB4.8 TCGCTTCCCTGCGAGGGAGGGC-----CAGGAGATCCAAAGGGAAGAACAAACACAGCAA 1673
hDUB8.2 TGGTTTACTGGGGAGGAAGAATTCCCTCAGGAGTGAAATTGGTGGCTCCTTCCTCCCTGT 1664
        * * ** *  * **** **        ******    *  **      *   * *
hDUB4.5 GAGGGCTCTGCTTGTGTG----CCAGTGGTCTCAGTGGAAGTACCGACCCACA        1722
hDUB4.8 GAGGGCTCTGCTTGTGTG----CCAGTGA------------------------        1698
hDUB8.2 CAAGTCTCTTCCTCAGGATTGCCCCTTTGTCTCTTCAGGACT-----------        1706
        * * **** * *  *      **  *
In frame termination codons in hDUB8.2 are underlined

TABLE 26
Deduced amino acid alignment of hDUB4.5 (Seq ID NO.14), hDUB4.8
(Seq ID NO.20) and hDUB8.2 (Seq ID NO.44).
hDUB4.5 MRQRARHLKTLSEGIASFCNLRSQQKNLVILVPVDMEEDSLYLGGEWQFNHFSKLTSSRP  60
hDUB4.8 MRQRARHLKTLSEGIASCCKLRSQQKNLVILVPVDMEDDSLYLGGEWQFNHFSKLTSSRP  60
hDUB8.2 MRPESPSFED-SEEIASFCNLRSQPKNLVILVPGDMEDDSLYLGGEWQFNHFSKLTSSRP  59
        ** .:  ::  ** *** *:**** ******** ***:**********************
hDUB4.5 DAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQLAPREKLPLSNRRPAAVGAGLQNM 120
hDUB4.8 DAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQLAPREKLPLSNRRPAAVGAGLQNM 120
hDUB8.2 DAAFAEIQRTSLSEKSSLSSETRVDLCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQNM 119
        ************.***.**.**************************.*************
hDUB4.5 GNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRHKGCMLCTMQAHITRALHNPGHVIQP 180
hDUB4.8 GNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRHKGCMLCTMQAHITRALHNPGHVIQP 180
hDUB8.2 GNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRHKCCMLCTMQAHITWPLHSPGHVIQP 179
        ************************************ *********** .**.*******
hDUB4.5 SQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQI 240
hDUB4.8 SQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQI 240
hDUB8.2 SQVLAAGFHRGEQEDAHEFLMFTVDAMKKAFLPGHKHLDNHSKDTTLIHQIFGGYWRSHI 239
        **.********:****************** *****::*:******************:*
hDUB4.5 KCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPASK 300
hDUB4.8 KCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPASK 300
hDUB8.2 NCFHCHGISDTFDPYLDIALDIQAAQSVKQAL---------------------------- 271
        :*:*************************:***
hDUB4.5 TLTLHTSAKVLILVLKRFSDVTGNKIDKNVQYPECLDMKLYMSQTNSGPLVYVLYAVLVH 360
hDUB4.8 TLTLHTSAKVLILVLKRFSDVTGNKIDKNVQYPECLDMKLYMSQTNSGPLVYVLYAVLVH 360
hDUB8.2 ------------------------------------------------------------
hDUB4.5 AGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASSITSVLSQQAYVLFYIQKSEWERHSESV 420
hDUB4.8 AGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASSITSVLSQQAYVLFYIQKSEWERHSESV 420
hDUB8.2 ------------------------------------------------------------
hDUB4.5 SRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNK 480
hDUB4.8 SRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNK 480
hDUB8.2 ------------------------------------------------------------
hDUB4.5 TKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTL 540
hDUB4.8 TKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTL 540
hDUB8.2 ------------------------------------------------------------
hDUB4.5 ASLRGRARRSKGKNKHSKRALLVCQWSQWKYRPT                           574
hDUB4.8 ASLRGRARRSKGKNKHSKRALLVCQ---------                           565
hDUB8.2 ----------------------------------
N-terminal potential mitochondrial targeting sequences are underlined.

TABLE 27
Upstream of initiation codon nucleotide sequence (putative
promoter region) alignment of hDUB4.5 (Seq ID NO.13), hDUB4.8
(Seq ID NO.19) and hDUB8.2 (Seq ID NO.43). Numbering is
initiated from initiation ATG.
hDUB4.5 CACACGAACACAATCACACACACACACTCACACGGTTTCCTACGTAAAGATTTCTTCCCT −276
hDUB4.8 CACACGAACACAATCACACACACACACTCACACGGTTTCCTACGTAAAGATTTCTTCCCT −276
hDUB8.2 GGGAGAAAAACACACACACACACACACACACACGGTTTCATAGGTAAAGATTTCTTCCCT −276
        *  ** ***  ************* *********** ** *****************
hDUB4.5 GCCATTGCTTTACCTAAAATAAGGCAACTGTGTGGCCACTGTCCCAACCCGGTTACACTC −216
hDUB4.8 GCCATTGCTTTACCTAAAATAAGGCAACTGTGTGGCCACTGTCCCAACCCGGTTACACTC −216
hDUB8.2 GACATTGTTTTACCTAAAATAAGGCAACTGTGTGGCCACTGTCCCAACCCGGTTACACTC −216
        * ***** ****************************************************
hDUB4.5 CTATTATATGTGCCTATCATCCTGAGGAGTAATTTGATTCAGGTGTTCTGGAAGTCATGC −156
hDUB4.8 CTATTATATGTGCCTATCATCCTGAGGAGTAATTTGATTCAGGTGTTCTGGAAGTCATGC −156
hDUB8.2 ATATTACATGTGTCTATCAGCCTGAGGAGTAGTTTGATTCAGGTGTTCTAGAAGTCATGA −156
        ***** ***** ****** ************ ***************** ********
hDUB4.5 TGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTCCTTCC  −96
hDUB4.8 TGTGGGCTGTGTCTGTTGAATACCCAGCGATGCAAGGGGACACACCCTGTGACTCCTTCC  −96
hDUB8.2 TGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTCATTCC  −96
        ********************* ********************************* ****
hDUB4.5 TGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGGTGTTC  −36
hDUB4.8 TGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGGTGTTC  −36
hDUB8.2 TTAATTGAGTGCTGATATTTGATTGGTTTATCGCGCACCTGATGGGTGGGTGGGGTGTTC  −36
        * ************************ ***************** ***************
hDUB4.5 GCGGTTGGTGGGGTTGACTTACAGAAGGGCTGATG                             0
hDUB4.8 GCGGTTGGTGGGGGTGACTTACAGAAGGGCTGATG                             0
hDUB8.2 GCGGTTGGTGGGGGTGAGTTATATAAGGGCTGATG                             0
        ************* *** *** * ***********

TABLE 28
CLUSTAL W (1.81) multiple sequence alignment of core amino
acids of hDUBs (8.5: Seq ID NO.30; 8.7: Seq ID NO.34; 8.1:
Seq ID NO.26; 4.2: Seq ID NO.10; 4.3: Seq ID NO.12; 4.5:
Seq ID NO.14; 4.1: Seq ID NO.6; 8.3: Seq ID NO.28; 8.11:
Seq ID NO.38; 8.8: Seq ID NO.36; 8.6: Seq ID NO.32)
8.5  MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSEARVDLCDDLAPVAR  60
8.7  MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSEARVDLCDDLAPVAR  60
8.1  MGDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSETRVDLCDDLAPVAR  60
4.2  MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVAR  60
4.3  MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVAR  60
4.5  MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVAR  60
4.1  MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVAR  60
8.3  MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSQLSTETRVDFCDDLAPVAR  60
8.11 MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSQLSTETRVDFCDDLAPVAR  60
8.8  MEDDSLYLGGDWQFNHFSKLTSSRLDAAFAEIQRTSLSEKSPLSSETRFDLCDDLAPVAR  60
8.6  MEDDSLYLGGDWQFNHFSKLTSSRLDAAFAEIQRTSLSEKSPLSSETRFDLCDDLAPVAR  60
     * :*******:************* ************.*** ** *:*.*:*****.***
8.5  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYENASLQCLTYTPPLANYMLSREHSQTCQRP 120
8.7  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYENASLQCLTYTPPLANYMLSREHSQTCQRP 120
8.1  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYENASLQCLTYTPPLANYMLSREHSQTCQRP 120
4.2  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRH 120
4.3  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRH 120
4.5  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRH 120
4.1  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRH 120
8.3  QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYVNASQQCLTYTPPLANYMLSREHSQTCHRH 120
8.11 QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYVNASQQCLTYIPPLANYMLSREHSQTCHRH 120
8.8  QLAPRKKLPLSSRRPAAVGAGLQKIGNTFYVNVSLQCLTYTLPLSNYMLSREDSQTCHLH 120
8.6  QLAPRKKLPLSSRRPAAVGAGLQKIGNTFYVNVSLQCLTYTLPLSNYMLSREDSQTCHLH 120
     *****:* ***.***********::*** * *.* *****  **:**** **.****:
8.5  KCCMLCTMQAHITWALHSPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGH 180
8.7  KCCMLCTMQAHITWALHSPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGH 180
8.1  KCCMLCTMQAHITWALHSPGHVIQPSQALAAGFHRGKQEDVHEFLMFTVDAMKKACLPGH 180
4.2  KGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGH 180
4.3  KGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGH 180
4.5  KGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGH 180
4.1  KGCMLCTMQAHITRALHIPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMRKACLPGH 180
8.3  KCCMLCTMEAHITWPLHIPGHVIQPSQALAAGFHRGKQEAALEFLMFTVDAMKKACLPGH 180
8.11 KCCMLCTMEAHITWPLHIPGHVIQPSQALAAGFHRGKQEAALEFLMFTVDAMKKACLPGH 180
8.8  KCCMFCTMQAHITWALYRPGHVIQPSQVLAAGFHRGEQEDAHEFLMFTVDAMKKACLPGH 180
8.6  KCCMFCTMQAHITWALYRPGHVIQPSQVLAAGFHRGEQEDAHEFLMFTVDAMKKACLPGH 180
     * **:***:**** .*: *********.********:** . **********:*******
8.5  KQVDHHSKDTTLIHQIFGGCWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVKQALEQL 240
8.7  KQVDHHSKDTTLIHQIFGGCWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVKQALEQL 240
8.1  KQVDHHCKDTTLIHQIFGGCWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVKQALEQL 240
4.2  KQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQL 240
4.3  KQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQL 240
4.5  KQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQL 240
4.1  KQVDRHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQL 240
8.3  KQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFGPYLDIALDIQEAQSVKQALEQL 240
8.11 K----------------------QILILVWKRFSDVTG---------------------- 196
8.8  KQLDHHSKDTTLIHQIFGAYWRSQIKYLHCHGISDTFDPYLDIALDIQAAQSVKQALEQL 240
8.6  KQLDHHSKDTTLIHQIFGAYWRSQIKYLHCHGISDTFDPYLDIALDIQAAQSVKQALEQL 240
     *                      **  *  : :**. .
8.5  VKPEELNGENAYHCGLCLQRAPVSKTLTLHTFAKERILETQRPWVVTRHKLAKSVQYAES 300
8.7  VKPEELNGENAYHCGLCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKLAKNVQYPEC 300
8.1  VKPEELNGENAYHCGLCLQRAPASNTLTLHTSAKVLILVLKRFSDVAGNKLAKNVQYPEC 300
4.2  VKPEELNGENAYHSGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPEC 300
4.3  VKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPEC 300
4.5  VKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIDKNVQYPEC 300
4.1  VKPEELNGENAYHCGVCLQRAPASKTLTLHNSAKVLILVLKRFPDVTGNKIAKNVQYPEC 300
8.3  VKPEELNGENAYHC---------------------------------GNKIAKNVQYPEC 267
8.11 ------------------------------------------------NKIAKNVQYPEC 208
8.8  VKPKELNGENAYHCGLCLQKAPASKTLTLPTSAKVLILVLKRFSDVTGNKLAKNVQYPKC 300
8.6  VKPKELNGENAYHCGLCLQKAPASKTLTLPTSAKVLILVLKRFSDVTGNKLAKNVQYPKC 300
     :*: *.***.:.
8.5  LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEGQWYKMDDAKVTACSIT 360
8.7  LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEGQWYKMDDAKVTACSIT 360
8.1  LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEVQWYKMDDAEVTVCSII 360
4.2  LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTAASIT 360
4.3  LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTAASIT 360
4.5  LDMKLYMSQTNSGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASSIT 360
4.1  LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYSSYVKAQEGQWYKMDDAEVTASSIT 360
8.3  LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKVQEGQWYKMDDAEVTASGIT 327
8.11 LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKVQEGQWYKMDDAE------- 261
8.8  RDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASGIT 360
8.6  RDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASGIT 360
     **: **** *:*******************:*** ****.** ********:
8.5  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPEL 420
8.7  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPEL 420
8.1  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRAKQGELKRDHPCLQAPEL 420
4.2  SALSQQAYVLFYIQKSEWERHSESVSRGREPRALGTEDTDRRATQGELKRDHPCLQAPEL 420
4.3  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPEL 420
4.5  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPEL 420
4.1  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGVEDTDRRATQGELKRDHPCLQAPEL 420
8.3  SVLSQQAYVLFYIHKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDYPCLQVPEL 387
8.11 --------------KSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDYPCLQVPEL 307
8.8  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRPATQGELKRDHPCLQVPEL 420
8.6  SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRPATQGELKRDHPCLQVPEL 420
     *********************.***** *.*******:****.***
8.5  DERLVERATQESTLDHWRFPQEQNKTKPEFNVRKVEGTLPPNVLVIHQSKYKCGMKNHHP 480
8.7  DERLVERATQESTLDHWRFPQEQNKTKPEFNVRKVEGTLPPNVLVIHQSKYKCGMKNHHP 480
8.1  DEHLVERATQESTLDHWKFLQEQNKTKPEFNVGKVEGTLPPNALVIHQSKYKCGMKNHHP 480
4.2  DEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHP 480
4.3  DEHLVERATQESTLDRWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHP 480
4.5  DEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHP 480
4.1  DEHLVERATQESTLDHWKFLQEQNKTKPEFNVRRVEGTVPPDVLVIHQSKYKCRMKNHHP 480
8.3  DEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKLEGTLPPNVLVIHQSKYKCGMKNHHP 447
8.11 DEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKLEGTLPPNVLVIHQSKYKCGMKNHHP 367
8.8  DEHLVERATQESTLDHWKFPQKQNKTKPEFNVRKVEGTLPPNVLVIHQSKYKCGMKNHHP 480
8.6  DEHLVERATQESTLDHWKFPQKQNKTKPEFNVRKVEGTLPPNVLVIHQSKYKCGMKNHHP 480
     **:************:*:* *:********** ::***:**:.********** ******
8.5  EQQSSLLNLSSTTRTDQESVNTGTLASLQGRTRRSKGKNKHSKRALLVCQ           530
8.7  EQQSSLLNLSSTTRTDQESVNTGTLASLQGRTRRSKGKNKHSKRALLVCQ           530
8.1  EQQSSLLNLSSTTRTDQESMNTGTLASLQGRTRRAKGKNKHSKRALLVCQ           530
4.2  EQQSSLLNLSSSTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ           530
4.3  EQQSSLLNLSSSTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ           530
4.5  EQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ           530
4.1  EQQSSLLNLSSTTPTDQESMNTGTLASLRGRTRRSKGKNKHSKRALLVCQ           530
8.3  EQQSSLLNLSSTNPTDQESMNTGTLASLQGRTRRAKGKNKHCKRALLVCQ           497
8.11 EQQSSLLNLSSTNPTDQESMNTGTLASLQGRTRRSKGKNKHCKRALLVCQ           417
8.8  EQQSSVLNLSSTKPTDQESMNTGTLASLQGSTRRSKGNNKHSKRSLLVCQ           530
8.6  EQQSSLLNLSSTKPTDQESMNTGTLASLQGSTRRSKGNNKHSKRSLLVCQ           530
     *****:*****:. *.***:********:* :**:**:***.**:*****

TABLE 29
CLUSTAL W (1.81) multiple sequence alignment of putative promoter
sequences of hDUBs (upstream of ATG initiation codon)
8.9	TGACGTGTGTGAAAACTACAGTGTGATGAGCATGACTTGCAGACAGGTTATCGATTGGGC	60
8.10	-GACGTGTGTGAAAACTACAGTGTGACGAGCATGACTCGCAGACAGGTTATCGATTGGGC	59
8.3	----------------------------------------------GATATCAATACGGC	14
8.11	----------------------------------------------GATATCAATACGGC	14
4.2_a	-----------------------------------------------TTATCGATTGGGC	13
4.5	--------------------------TGAGCATGACTGGCAGACAGCTTATCGATTGGGC	34
4.3	------------------------GATGAGCATGACTGGCAGACAGCTTATCGATTGGGC	36
4.2_b	----------------------GTGATGAGCATGACTGGCAGACAGCTTATCGATTGGGC	38
4.4	----------------------GTGATGAGCATGACTGGCAGACAGCTTATCGATTGGGC	38
4.1_b	-------------------AGTGCAATGAGCATGACACGCAGGCAGGATATCAATTCGGC	41
8.1	-----------------------CAATGAGCATGACACGAAGACAGAATATCAATTCGGC	37
8.7	-----------------------CAATGAGCATGACACGAAGACAGAATATCAATTCGGC	37
8.6	---------------------TGTGATGAGCATGACTCGCAGACAGGTTATCGATTGGGC	39
8.8	---------------------TGTGATGAGCATGACTCGCAGACAGGTTATCGATTGGGC	39
8.5	-----------------TCTCTGTCAGAACCATGGTACTCTGTTGTGGTGTGAAAGTAGC	43
8.2	---------TCACATCTTCTCGGCCAGATGCAGCCTTTGCTGAAATCCAGCGGACTTCTC	51
4.1_a	----------------------------AGTATAGCAGAGCAGAGAGCTG-GAAGGGACC	31
	*     *
8.9	T-CCCCTCAAAAT-TAGTTATGAGCATTAAAGGACACCGATGCCC---AGGTCCCGGCTG	115
8.10	T-CCCCTCAAAAT-CAGTTATGAGCATTAAAGCACACCGATCCCC---AGGTCCCGGCTC	114
8.3	T-CACCTCAAAAG-CAGTTATGAGCATTAAAGGACACCCATGCCT---AGGTCCCGCTTA	69
8.11	T-CACCTCAAAAG-CAGTTATGAGCATTAAAGGACACCCATGCCT---AGGTCCCGGTTA	69
4.2_a	T-CCCCTCAAAAT-CGGTTATGAGCATTCAAGCACACCGATGCCC---AGGTCCCGGCTG	68
4.5	T-CCCCTCAAAAT-CGGTTATGAGCATTCAAGCACACCGATGCCC---AGGTCCCGGCTG	89
4.3	T-CCCCTCAAAAT-CGGTTATGAGCATTCAAGCACACCGATGCCC---AGGTCCCGGCTG	91
4.2_b	T-CCCCTCAAAAT-CGGTTATGAGCATTCAAGCACACCGATGCCC---AGGTCCCGGCTG	93
4.4	T-CCCCTCAAAAT-CGGTTATGAGTATTCAAGCACACCGATGCCC---AGGTCCCGGCTG	93
4.1_b	T-CCCCTCAAAAG-CTGTTATGAGCATTAAAGGACACCAATGCCT---AGTTCCCGGTTA	96
8.1	T-CACCTCAAAT--CAGTTGTGAGCATTAAAGAAAACCAATTCCT---AGGTCCCGCTTA	91
8.7	T-CACCTCAAAAG-CAGTTATGAGCATTAAAGGACAACAATTCCT---AGGTCCCGCTTA	92
8.6	T-CCCCTCAAAAT-CAGTTAGGAACATGAAAGCACACCGATGCCC---AGGTCCTGGCTG	94
8.8	T-CCCCTCAAAAT-CAGTTAGGAACATGAAAGCACACCGATGCCC---AGGTCCTGGCTG	94
8.5	CACAGATCATCTG-TAGAT-TAAGGGGTGTGGCTTTGTTCCAACA---AAGCTTTATTTA	98
8.2	TCCCTGAGAAGTCACAACTCTCAACTGAGACCCGCGTCGACTTCTGCGATGATTTGGCGC	111
4.1_a	TGCATCCCTAAT----GATATAAGAAAGTATCTGTACTAGCCCTGA--ATGGTATAACTA	85
	*               *   *                         *
8.9	CAGGAATAAGAC-CCTCCGACGTCTTGTGTGAAGCCACGGC--ATCTGGATTGCTCATGC	172
8.10	CAGGAATAAGAC-CCTCCAGCGTCTTGTGTGAAGCCACGGC--ATGTGGATTGCTCATGC	171
8.3	AAGAGATAAGAC-TCTCCCACACCCTGTGTGAAGCCACGGC--ATGTGGATTGCTCATGC	126
8.11	AAGAGATAAGAC-TCTCCCACACCCTGTGTGAAGCCACGGC--ATGTGGATTGCTCATGC	126
4.2_a	CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC--ATCTGCATTGCTCATGC	125
4.5	CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC--ATCTGCATTGCTCATGC	146
4.3	CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC--ATCTGCATTGCTCATGC	148
4.2_b	CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC--ATCTGCATTGCTCATGC	150
4.4	CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC--ATCTGCATTGCTCATGC	150
4.1_b	AAACGATAAGAC-TCTCGCACACCCTGTGGGAAGCCACGGC--ATCTGGATTGCTCATGC	153
8.1	AAGAGATAAGAC-CATCCAACAACCTGTGTGAAGCCACCGC--ATCTGGCTTGCTCATGA	148
8.7	AAGAGATAAGAC-CATCCAACACCCTGTGTGAAGCCACGGC--ATCTGGATTGCTCATGT	149
8.6	CAGGAATAAGAT-CCTCCGACGTCTTGTGTGAAGCCACGAC--ATCTGCATTGCTCATGC	151
8.8	CAGGAATAAGAT-CCTCCGACGTCTTGTGTGAAGCCACGAC--ATCTGCATTGCTCATGC	151
8.5	CAAACACAGGCTGTGGGCTGGATTTGGCCTGCAGCTGTAGT--TTGTG----ATCCTTGA	152
8.2	CTGTGGCAAGAC--AGCTTGCTCCCAGGGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGC	169
4.1_a	CAG-GTTAAATT---TACGTGAAAAAGAAATCAACTTCTGCCTTGTTTAAGCAAACTTA-	140
	*                  *     * *                    * *
8.9	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTCCTGCCTGTGGAGCACCTCT	228
8.10	TTCTG-G-CGATCATTCTCCTGAAAACG--GTGGCTCCTTTCTCCCTGTGGAGCACCTTT	227
8.3	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTTCTCACTGTGGAGCATCTTT	182
8.11	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTTCTCACTGTGGAGCATCTTT	182
4.2_a	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTTCTCCCTGTGGACCATCTTT	181
4.5	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTTCTCCCTGTGGAGCATCTTT	202
4.3	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTTCTCCCTGTGGAGCATCTTT	204
4.2_b	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTTCTCCCTGTGGAGCATCTTT	206
4.4	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTCCTTTCTCCCTGTGGAGCATCTTT	206
4.1_b	TTCTG-G-GGATCATTTTCCTGAAAATC--GTGGCTGCTTTCTCCCTGTGTAGCATCTTT	209
8.1	TTCTG-G-GGATCATTCTCCAGAAAATG--GTGGCTCCTTTCTCCCTGTGGAGCATCTTT	204
8.7	TTCTG-G-GGAACATTCTTCTGAAAATG--GCGGCTCCTTTCTCCCTGTGGAGCATCTTT	205
8.6	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTTCTTTCTCCCTGTGGAGCATCTTT	207
8.8	TTCTG-G-GGATCATTCTCCTGAAAATG--GTGGCTTCTTTCTCCCTGTGGAGCATCTTT	207
8.5	TTCAG-ACAGTTTAGCAAGGCTGAAAAG--AACACCCACACCCCCTTGTTACCCACAGAT	209
8.2	TGCGGTGGGGGCTGGGCTCCAGAATATG--GGAAATACCTGCTACGTG---AACGCTTCC	224
4.1_a	TTCAG---GCATTAATTTTATAAATATGTAGAGAATACATACTCCTTAT-GAGCAGA---	193
	* * *                  * *               *    *      *
8.9	CTA-AGCA-GTGC-CCTTTCTTCACCCAGGACACTTTACATCAGGCACAGAAAGCCTTCT	285
8.10	CTA-AGCA-GTGC-CCTTTCTTCACCCAGGACACTTTACATCAGGCACAGAAAGCCTTCT	284
8.3	GTA-AGCA-GTGT-CCTTTCTTCCCCCAGGACACTTTACTTCAGGCACAGGAAGCCTTCT	239
8.11	CTA-AGCA-GTGT-CCTTTCTTCCCCCAGGACACTTTACTTCAGGCACAGGAAGCCTTCT	239
4.2_a	CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT	239
4.5	CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT	260
4.3	CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT	262
4.2_b	CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT	264
4.4	CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT	264
4.1_b	CTA-AGCA-GTGCTCCTTTCTTCCCACAGGAAACTTTACATCAGGCACAGGAAGCTTTCT	267
8.1	CTA-AGCA-GTGC-CCTTTCTTCCCCCAGGACACTTTACATGAGGTGCAGGAAGCCTTCT	261
8.7	CTA-AGCA-GTGCTCCTTTCTTCCCCCAGGACACTTTACATCAGGCATAGGAAGCCTTCT	263
8.6	CTA-AGCA-GTGCTCCTTTCTTCCCCCAGGACACTTTACATCAGGCGCACGAAGCCTTCT	265
8.8	CTA-AGCA-GTGCTCCTTTCTTCCCCCAGGACACTTTACATCAGGCGCACGAAGCCTTCT	265
8.5	GGGTGGGA-CTGTGTTGGCCAGAGACCGAGAGACGGGTGCTCACAGGGGAACGTACAGCA	268
8.2	CAGCAGTGTCTGACATACACACCGCCCCTTGCCAACTACATGCTGTCCCGGGAGCACTCT	284
4.1_a	-AACAATGTTTGCGCCATATGGTCCATGATGGGTGTTCAATAATGTGTGATGATGATAAT	252
	**                            *
8.9	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGATAAGAAACGGGGCCAAACATCACAG	345
8.10	GATGGAGCACACCTGGCCCATGAAAAAACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	343
8.3	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAATGGGGCCAAAGGTCACAG	298
8.11	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAATGGGGCCAAAGGTCACAG	298
4.2_a	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	298
4.5	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	319
4.3	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	321
4.2_b	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	323
4.4	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	323
4.1_b	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAATGGGGCCAAAGGTCACAC	326
8.1	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAAAAGGGCCAAAGGTCACAG	320
8.7	GATGAAGTACACCTGGCCCATGAAAAGACAAAGGA-AAGAAACAGGGCCAAAGGTCACAG	322
8.6	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	324
8.8	GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG	324
8.5	TGTAGAGGCCGGAAGGTGCTCCAGGGCACAAGTGT-GGGAAAGTGGGACATACGGGGAAG	327
8.2	CAAACATGTCATC--GTCACAAGTGCTGCATGCTCTGTACCATGGAAGCTCACATCACAT	342
4.1_a	AATGAAGACAATAGTGACAAATAAAAGAAAATAAA-AAGCAGTGAAACAAAGTGGTTTAA	311
	*         *             *                            *
8.9	TCCTCTCATTCCACCGTCCTCCTTAAAATCATCCTAATTTCATGGGCTCT-GCGGCCACG	404
8.10	TCCTCTCATTCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAGGCCACG	402
8.3	TCCTCTCATTCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG	357
8.11	TCCTCTCATTCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAGGCCACG	357
4.2_a	TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG	357
4.5	TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG	378
4.3	TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG	380
4.2_b	TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG	382
4.4	TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG	382
4.1_b	TCCTCTCATTCCATCATACTCCTTAAAATCATCCTAATTTCATGGGTCCT-GAAGCCAGG	385
8.1	TCCTCTCATTACATCATCATCCTTAAAATCATCCTAATTTCATGAGCCCT-GAAGACAGG	379
8.7	CCCACTCATTTCATCACCATCCTTAAAATCATCCTAATTTCATGGGCCAT-GAAGCCAGG	381
8.6	TCCTCTCATTCCATCATCCTCCTTAAAATCATCCGAATTTCATGAGCCCTTGAAGCCAGG	384
8.8	TCCTCTCATTCCATCATCCTCCTTAAAATCATCCGAATTTCATGAGCCCTTGAAGCCAGG	384
8.5	TTTCCAGAAAGCATGATGTCAAGTTGGAG-GTGGAGCGCTGCTGGGCTTGTGAAGGGTCT	386
8.2	GGCCCC---TCCA-CATTCCTGGCCATGTCATCCAGCCCTCACAGGCATT---------G	389
4.1_a	TAGCTATACATAGTTATT-TTGTTGAAAGATTCTGCTGCTAATATTATTCAATATTTTTG	370
	*       *
8.9	GCTGTTTCTTTACACCTCGAGACCTTGGCGCCAGGCCTCAATTCTGCCCCGGTGCTTACT	464
8.10	GCTGTTTCTTTACACCTCGAGACCTTGGCGCCGGGCCTCAATTCTGCTCCAGTGCTTACT	462
8.3	GCTGTTTCTTTAAAACTAGAGGCCTTGGCGCCGTGCCTCAATTCTGCCCTGTTCCTTACT	417
8.11	GCTGTTTCTTTAAAACTAGAGGCCTTGGCGCCGGGCCTCAATTTTGCCCTGTTCCTTACT	417
4.2_a	GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC	417
4.5	GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC	438
4.3	GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC	440
4.2_b	GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC	442
4.4	GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC	442
4.1_b	GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTGCTTACT	445
8.1	GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTCCTTACT	439
8.7	GCTGTTTCTTTACACCCAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTCCTTACT	441
8.6	GCTGTTTCTTTACACCCAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTCCTTACT	444
8.8	GCTGTTTCTTTACACCCAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTCCTTACT	444
8.5	CGAGTCCAAGTGAGGGCGGGTTGTGAAGGGTCTCCTCTCAAAGCTGACCGACTTCGGGAC	446
8.2	GCTGCTGGCTTCCATAGAGGCAAGCAGGAAGCTGCCCTTGAATTTCTCATGTTCACTGTG	449
4.1_a	TATGCTGGC--GCAAATAAGGAAATTTACATCGTCTAATAAAAATTATTTATCAATTTAT	428
	*                           *        *
8.9	GTCTAAGACATTTTGGGAAAATCCCTAGAGC-CTGGATCTTCAATCCTGGTAAGCCAGAG	523
8.10	GTCTAAGACATTTTGGGAGAATCCCTAGAGC-CTAGATCTTCAATCCTGGTAAGCCAGAG	521
8.3	GTCTAAGAAAGGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG	476
8.11	GTCTAACAAAGGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG	476
4.2_a	GTCTAAGACATGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGCTAAGCCAGAC	476
4.5	GTCTAAGACATGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGCTAAGCCAGAC	497
4.3	GTCTAAGACATGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGCTAAGCCAGAC	499
4.2_b	GTCTAAGACATGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGCTAAGCCAGAC	501
4.4	GTCTAAGACATGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGCTAAGCCAGAC	501
4.1_b	CTCTAAGACATGTTGGGAAAGTCCCAAGAGC-CAGGATCTTCATTCCTGGTAAGGCAGAC	504
8.1	GTCTAAGACATTTTGGGAAAATCACTAGAGC-CAGGACCTTCATTCCTGGTAAGCCAGAG	498
8.7	GTCTAAGACATTTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG	500
8.6	GTCTAAGACATTTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG	503
8.8	GTCTAAGACATTTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG	503
8.5	TTCCCAGGGATTGTTGTTGAGTCCACGGCTCACGTCGTCCACACTC-TGAGGTCCCATGG	505
8.2	GATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGCAGGTAGATC--ATCACTCCAAGG	507
4.1_a	AAAACAGTAAAAATTTCATAG--AATGGGGCTAAGAATCTGCACTGCAAACTAACTCTTT	486
	*                 *  *             *
8.9	AGCCTGGAGACACACCCAAATTATGTCCCTCTTAGTTCAGGGAACATGTCCATTTTCGTC	583
8.10	AGCCTGAAGACACACCCAAATTATGTCCCTCTTAGTTCAGGGAACATGTCCATTTTCGTC	581
8.3	AGCCTGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGTC	536
8.11	AGCCTGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGTC	536
4.2_a	AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC	536
4.5	AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC	557
4.3	AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC	559
4.2_b	AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC	561
4.4	AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC	561
4.1_b	AGAATGAAGACACACCAAAATTCTGTCCCTCTTAATTCAGGGAACGTGTCCACTTTCGTC	564
8.1	AGCCGAAAGACACACCCAAATTCTGTCCCTCTTAGTTCAGGGAACAGGTCTACTTTCGTC	558
8.7	AGCCTGAAGACACACCCAAATGCTGTCCCTCTTAGTTCAGGGAACATGTCCACTTTCGTC	560
8.6	AGCCTGAAGACACACCCAAATGCTGTCCCTCTTAGTTCAGGGAACATGTCCACTTTCGTC	563
8.8	AGCCTGAAGACACACCCAAATGCTGTCCCTCTTAGTTCAGGGAACATGTCCACTTTCGTC	563
8.5	ATTCGCCACCCACATTCATCTACTCTCCTCTCTCTCTTCCTTACCTCCTTTGATCCCCTC	565
8.2	ACACCACCCTCATCCACCAAATATTTGGAGGGTACTGGAGATCTCAAATCAAGTGTCTCC	567
4.1_a	CAGTTGATTTTATGCACAGAAATTATTGAGAATCCCCTTATCTAGATCCAACAGATCTGG	546
	*            * *     *                       *
8.9	AGCATTAAAATTTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCATAACTG	643
8.10	AGCACTAAAATTTTTGCACCAAATGTGCTAACTGCAATTCCACCATGCAATGCGTAACTG	641
8.3	AGCATTACAATTTTTGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCATAAATG	596
8.11	AGCATTACAATTTTTGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCATAAATG	596
4.2_a	AGCATTACAATTTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCGTAACTG	596
4.5	AGCATTACAATCTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCGTAACTG	617
4.3	AGCATTACAATTTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCGTAACTG	619
4.2_b	AGCATTACAATTTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCGTAACTG	621
4.4	AGCATTACAATTTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCGTAACTG	621
4.1_b	AGAATTAAAATTTTTGCACCAAATGTGCTAACTGGAATTCCACCATACAATGCATAACTG	624
8.1	AGCATTACAATTTTTGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCATAACTG	618
8.7	AGCATTACAATTTTTGCACCAAATGTACTAACTGCAATTCCACCATACAATGCATAACTG	620
8.6	AGCATTACAATTTTTGCACCAAATGTACTAACTGCAATTCCACCATACAATGCATAACTG	623
8.8	AGCATTACAATTTTTGCACCAAATGTACTAACTGCAATTCCACCATACAATGCATAACTG	623
8.5	CCCA-TATGCTACCCCCCGCTACCAAACCTCTGCCAAGCATACCACCCTATCGGCAGCTA	624
8.2	ACTGCCACGGCATTTCAGACACTTTTGGCCCTTACCTGGACATCGCCCTGGATATCCAGG	627
4.1_a	GCTTACATAGGTGCTATCAAGACTTAAGGAAGAAAATTTTCCTGACTCTATCCATACCTC	606
	*                                        *
8.9	GAAATAGAGGCAACATCTCATATCCTGAACAATTCATGTG------AGAATCTAGGAGAC	697
8.10	TAAATGGAGGCAACATCTCAGATCCTGAACAATCGATGCG------AGAATCCAGGAGAC	695
8.3	GAAATGGAGGGAACATCTCAGATCCTGAACAATCGATGCG------AGAATCCAGGAGAT	650
8.11	GAAATGGAGGGAACATCTCAGATCCTGAACAATCGATGCG------AGAATCCAGGAGAT	650
4.2_a	GAAATGGAGGCAACATCTCCGATCCTGAACGATCGATGCG------AGAATCCAGGATAT	650
4.5	GAAATGGAGGCAACATCTCCGATCCTGAACGATCGATGCG------AGAATCCAGGATAT	671
4.3	GAAATGGAGGCAACATCTCCGATCCTGAACGATCGATGCG------AGAATCCAGGATAT	673
4.2_b	GAAATGGAGGCAACATCGCCGATCCTGAACGATCGATGCG------AGAATCCAGGATAT	675
4.4	GAAATGGAGGCAACATCTCCGATCCTGAACGATCGATGCG------AGAATCCAGGATAT	675
4.1_b	GAAATGGAGGGAAAATCCCAGATCATGAACAATCAAAGCG------AGAATCCAGGAGAC	678
8.1	GAAATGGAGGGAACATCTCAGATCATGAACAATCGATGAG------AGAATCCAGGAGAT	672
8.7	GAAATGGAGGGAACATCTCAGAGCATGAACAACTGATGCG------AGAATCCAGGAGAT	674
8.6	GAAATGGAGGGAACATCTCAGACCATGAACAATCGATGAG------AGAATCCAGGAGAC	677
8.8	GAAATGGAGGGAACATCTCAGACCATGAACAATCGATGAG------AGAATCCAGGAGAC	677
8.5	CTCTTCACTCCCA-ACTACATCGGCCGCATCNNNNNNNNN------NNNNNNNNNNNNNN	677
8.2	AAGCTCAGAGTGTCAAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGAACTCAATGGAG	687
4.1_a	CAATTAGTAATAGATCTAGAGATTTAAAACTGAAATCCAGACCTC-CTGCTTCCATGTGC	665
	*                       *
8.9	ACACCGCTTATTTTTGCCTTTTCCCACTGAAACAATGGCTAGTATTAACAATGTTATGCT	757
8.10	ACACGGCTTATTTTTGCCTTTTCCCACTGAAACAAGGGCCAGTATTAACAATCTTATGCT	755
8.3	ACACGGCTGATTTTTGCGTTTTCCCTGTGAAACAAGGGCCAGTATTAAAAATGGTATGCT	710
8.11	ACACGGCTGATTTTTGCGTTTTCCCTGTCAAACAAGGGCCAGTATTAAAAATGGTATGCT	710
4.2_a	GCACGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGCACGCT	710
4.5	GCACGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGCACGCT	731
4.3	GCACGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGCACGCT	733
4.2_b	GCACGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGTACGCT	735
4.4	GCACGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGCACGCT	735
4.1_b	ACACGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGACCAGTATTAAAAATGGTATGCT	738
8.1	ACACGGCTTATTTTTGCCTTTTCCCTGTGAAACAAGGGCAAGTATTAAAAACTTTATGCT	732
8.7	ACACGGTTTATTTTTGCCATTTCCCAGTGAAACAAAAGCCAGTATTAAAAAGGTTATGCT	734
8.6	ACACGGCTTATTTTTGCCTTTTCCCTGTGAAACAAGGGCCAGCATTAAAAAGGTTATGCT	737
8.8	ACACGGCTTATTTTTGCCTTTTCCCTGTGAAACAAGGGCCAGCATTAAAAAGGTTATGCT	737
8.5	NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	737
8.2	AGAATGCCTATCATTGTGGTCTTTGTCTCCAGAGGGCGCCGGCCTCCAAGACGTAAACTT	747
4.1_a	AGTGTCCTTTCACTGTCCTGTTTTGCTTCACTTGATGAAGAGGATTTGAGAATAAATGAC	725
8.9	ATCCTTGGTTTCACTCCCCACTTTTAAATCTCTCGGATGTTTACTTCT-TGAGACAGG--	814
8.10	ATCCTGGGTTTCACTCTCTGCTTTTAAATCTCTCCAATGTTTTCTTCT-TGAGACAGG--	812
8.3	ATCCTCTGTTTCACTCCCTGCTTTTAAGTCTCC--GATGTTT-CTTCT-TAAGACAGG--	764
8.11	ATCCTCTGTTTCACTCCCTGCTTTTAAGTCTCC--GATGTTT-CTTCT-TAAGACAGG--	764
4.2_a	ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCC-TGAGACAGG--	766
4.5	ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCC-TGAGACAGG--	787
4.3	ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCC-TGAGACAGG--	789
4.2_b	ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCC-TGAGACAGG--	791
4.4	ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCTGATGT-TTCTCCC-TGAGACAGG--	791
4.1_b	ATACTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTTCT-TGAGACAGG--	794
8.1	GTCTTCTATTTCACTGCCTGCTTTTAAACGTCTCCGATGTATTCTTCT-TGAGAAAGG--	789
8.7	ACCCTCTGTTTCACTCACTGCTTTTAAACGTCTCCGATGTTTTCTTCT-TCAGACAGG--	791
8.6	ATCTTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGTTTTCTTCT-TCAGACAGG--	794
8.8	ATCTTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGTTTTCTTCT-TCAGACAGG--	794
8.5	NNNNNNNNNNNNNNNNNCTGCTTTTAAACGTCTCCGATGTTTTCTTCT-TCAGACAGG--	794
8.2	TACACACTTCTGCCAAGATCCTCATCCTCGTATTGAAGAGATTCTCCGATGTCACAGGCA	807
4.1_a	CACATGATTCAACTCCTCCTCAGCTCTGAGCAAT-ATAGCCCTGTCCTGGCAAACAAGAA	784
	*   *                   * *      * * *
8.9	GCGTC-ACTGCCGTCACCCACGCTTTTCTACGGT------GTAATTTTTGTTGTTCGCTT	867
8.10	GCCTC-ACTCCCGTCACCAGGGCTTTTCTACGGT------GCAATTTTCGGTGTTTGCTT	865
8.3	GCCTC-ACTTCCTTCCCCCTGACTTTTCTACGGT------ATAATTTTCGTTGTTTGCTT	817
8.11	GCCTC-ACTTCCTTCCCCCTGACTTTTCTACGGT------ATAATTTTCGTTGTTTGCTT	817
4.2_a	GCCTC-ACTTCCGTCAGCCGGGCTTTTCCACGGT------ATAATTTTCCTTGTTTGCTT	819
4.5	GCCTC-ACTTCCGTCAGCCGGGCTTTTCCACGGT------ATAATTTTCCTTGTTTGCTT	840
4.3	GCCTC-ACTTCCGTCAGCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT	842
4.2_b	GCCTC-ACTTCCGTCAGCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT	844
4.4	GCCTC-ACTTCCGTCAGCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT	844
4.1_b	GCCTC-ACTGCCGTCCGCCGGGCTAT-CTAGAGT------ATAATTTTCAGTGTTTGCTT	846
8.1	GCCTC-ACTACTGTCACCTGGGCTTTTCTAAGGT------ATAATTTTCCTTGTTTGCTT	842
8.7	GCCTC-ACTCCCGTCACCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT	844
8.6	GCCTC-ACTCCCGTCACCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT	847
8.8	GCCTC-ACTCCCGTCACCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT	847
8.5	GCCTC-ACTCCCGTCACCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT	847
8.2	ACAAA-ATTGCCAAGAATGTGCAATATCCTGAGTGCCTTGACATGCAGCCATACATGTCT	866
4.1_a	GCTCCTGCAGTAGTAGAGGAGGCAAATATACGTT----CACTAATCTAACATACAAG---	837
	*                               *        *        *       *
8.9	TTGTCAAATTTAGAAATTTTCATTTCA--TCTCTATCAAATGTTGCTCCATT----ATCA	921
8.10	TTGTCAAATTTAGAACTTTTCATTTCA--TCTCTATCAAATGTTGATCCATT----ATCA	919
8.3	TTGTCAAAATTAGAACTTTTTATTTCA--TCTCTATGAAATGTTGATCCATT----ATCA	871
8.11	TTGTCAAAATTAGAACTTTTTATTTCA--TCTCTATGAAATGTTGATCCATT----ATCA	871
4.2_a	TTGTCCAAATTAGAACTTTTTATTTCA--CCTCTAGGAAACGTTGATCCATT----ATCA	873
4.5	TTGTCCAAATTAGAACTTTTTATTTCA--CCTCTAGGAAACGTTGATCCATT----ATCA	894
4.3	TTGTCCAAATTAGAACTTTTTATTTCA--TCTCTAGGAAACGTTGATCCATT----ATCA	896
4.2_b	TTGTCCAAATTAGAACTTTTTATTTCA--TCTCTAGGAAACGTTGATCCATT----ATCA	898
4.4	TTGTCCAAATTAGAACTTTTTATTTTA--TCTCTAGGAAACGTTGATCCATT----ATCA	898
4.1_b	TTGTCAACCTTAGAACATTTTATTTCG--TCTCTATGAAATGTTGATCCATT----ATCA	900
8.1	TTGTCAAAATTAGAACATTTTATTTCA--TATCTATGAAATGTTGATCCATT----ATCA	896
8.7	TTGTCAAAATTAGAACTTTTTATTTCA--TCTCTATGAAATGTTGATCCATT----ATCA	898
8.6	TTGTCAAAATTAGAACTTTTTATTTCA--TCTCTATGAAATGTTGAGCCATT----ATCA	901
8.8	TTGTCAAAATTAGAACTTTTTATTTCA--TCTCTATGAAATGTTGAGCCATT----ATCA	901
8.5	TTGTCAAAATTAGAACTTTTTATTTCA--TCTCTATGAAATGTTGATCCATT----ATCA	901
8.2	CAGCAGAACACAGGACCTCTTGTCTATGTCCTCTATGCTGTGCTGGTCCACGCCGGGTGG	926
4.1_a	--GCAGTAGGCACTGTACCATAAACAAG-ACACTGTGGGGGTTCAGACCAGG---GGCAA	891
	*        *                    **             ***
8.9	CATACGTATGA-AAATATTATCACGCGTGCTGTGAGATACGTTGTTTTTATTTTCATCAA	980
8.10	CATACGTATGA-AAATATTATCACCCATGCTGTGAGATACGTTGTTTTTATTTTCATCAA	978
8.3	CATACGTATGG-AAAGACTATCACCCATGCTGTGAGATACGTTGTTTTTATTTTCATCAA	930
8.11	CATACGTATGG-AAAGACTATCACCCATGATGTGAGATACGTTGTTTTTATTTTCATCAA	930
4.2_a	CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA	932
4.5	CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA	953
4.3	CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA	955
4.2_b	CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA	957
4.4	CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA	957
4.1_b	CATACACATGG-AAATATTATCACCCACGGTGTCAGATACGTTGTTTTTATTTTCATCAC	959
8.1	CATACGTATGG-AAATAGTATCACCAATGCTGTGAGATAAGTTGTTTTTATTTTGGTCAA	955
8.7	CGTAAGTATGG-AAATAGTATCAGCCACGCTGTGAGATACGTTGTTTTTATTTTCATCAG	957
8.6	CATACGTATGG-AAACAGTATCACCCATGCTGTGAGATACGTTGTTTTTATTTTCATCAG	960
8.8	CATACGTATGG-AAACAGTATCACCCATGCTGTGAGATACGTTGTTTTTATTTTCATCAG	960
8.5	CGTAAGTATGG-AAATAGTATCAGCCACGCTGTGAGATACGTTGTTTTTATTTTCATCAG	960
8.2	AGTTGTCACAACGGACATTACTTCTCTTATGTCAAAGTTCAAGAAGGCCAGTGGTATAAA	986
4.1_a	AGTGGGGATTG---ATAGGGCTAGTAAAGTCTAGGAAGTGTTCACTAACAAAATGTCTAA	948
	*    *      * *                                *        *
8.9	TTCTTTTGTAAAACAAAGGTTATAGTTGGGATACCTTCTGATTTCTCAAGTTTTTTGTTT	1040
8.10	TTCTTTAATAAACCAAAGGTTATAGTTGGGATACCTTCTGATTTCTCAAGTTTTTTGTTT	1038
8.3	TTCTTTAATAAACCAAAGGTTATAGTTGGGATACCTTCCGATTTCTCTAGTTTTTTGTTT	990
8.11	TTCTTTAATAAACCAAAGGTTATAGTTGGGATACCTTCCGATTTCTCTAGTTTTTTGTTT	990
4.2_a	TTCTTTAATAAACAAACGGTTATAGCTGGGATACCTTCTGAGTTCTCAAGTTTTTTGTTT	992
4.5	TTCTTTAATAAACAAACGGTTATAGCTGGGATACCTTCTGAGTTCTCAAGTTTTTTGTTT	1013
4.3	TTCTTTAATAAACAAAAGGGTATAGCTGGGATACCTTCTGAGTTCTCAAGTTTTTTGTTT	1015
4.2_b	TTCCTTAATAAACAAAAGGTTATAGCTGGGATACCTTCTGAGTTCTCAAGTTTTTTGTTT	1017
4.4	TTC-TTAATAAACAAAAGGTTATAGCTGGGATACCTTCTGAGTTCTCAAGTTTTTTGTTT	1016
4.1_b	TTC---AAGAAAAAAAAGGGTATAGTTGGGATACCTTCTGATTTCTCAAGATTTTTCTTT	1016
8.1	TTCTTTAATAAACAAAAGCTTATAGTTGGGATACCTTCTGATTTCTCAAGGTTTTTGTTT	1015
8.7	TTATTTAATAAACAAAAGCTTATAGTTGGGATACCTTTGGATTTCTCAAGTTTTTTGTTT	1017
8.6	TTCTTTAATAAACAAAAGCTTATAGTTGGGATACCTTTGGATTTCTCAAGTTTTTTGTTT	1020
8.8	TTCTTTAATAAACAAAAGCTTATAGTTTGGATACCTTTGGATTTCTCAAGTTTTTTGTTT	1020
8.5	TTATTTAATAAACAAAAGCTTATAGTTGGGATACCTTTGGATTTCTCAAGTTTTTTGTTT	1020
8.2	ATGGATGATGCCGAGGTCACTGCCTCTGGCATCACCTCTG---TCCTGAGTCAACAGGCC	1043
4.1_a	TTATTAACTAAACTAAATGGTTTC-TCAACATGACCTAATTAATTGTAACTTACTATAAA	1007
	*                  *         **  * *      *    *
8.9	CATGTTTTCTT-------------------------------------------------	1051
8.10	CAGGTTTTCTT-------------------------------------------------	1049
8.3	CATGTTTTCTTTCTTTTTTTTTTTTTTTTTTTT---------GAGACGGGGTCTCGCTCT	1041
8.11	CATGTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTCGCTCT	1050
4.2_a	CGTGTTTTCTT-------------------------------------------------	1003
4.5	CGTGTTTTCTT-------------------------------------------------	1024
4.3	CGTGTTTTCTT-------------------------------------------------	1026
4.2_b	CGTGTTTTCTT-------------------------------------------------	1028
4.4	CCTGTTTTCTT-------------------------------------------------	1027
4.1_b	CATATTTTCTT-------------------------------------------------	1027
8.1	CATGTTTTCTT-------------------------------------------------	1026
8.7	CAAGTTTTCTT-------------------------------------------------	1028
8.6	CATGTTTTCTT-------------------------------------------------	1031
8.8	CATGTTTTCTT-------------------------------------------------	1031
8.5	CAAGTTTTCTT-------------------------------------------------	1031
8.2	TATGTCCTCTTTTACATCC-----------------------------------------	1062
4.1_a	TGGTTGTTTGTTCA----------------------------------------------	1021
	*  *  *
8.9	------------------------------------------------------------
8.10	------------------------------------------------------------
8.3	GTCGCCCAGGCCGGACTGCGGACTGCAGTGGCGCAATCTCGGCTCACTGCAAGCTCCGCT	1101
8.11	GTCGCCCAGGCCGGACTGCGGACTGCAGTGGCGCAATCTCGGCTCACTGCAAGCTCCGCT	1110
4.2_a	------------------------------------------------------------
4.5	------------------------------------------------------------
4.3	------------------------------------------------------------
4.2_b	------------------------------------------------------------
4.4	------------------------------------------------------------
4.1_b	------------------------------------------------------------
8.1	------------------------------------------------------------
8.7	------------------------------------------------------------
8.6	------------------------------------------------------------
8.8	------------------------------------------------------------
8.5	------------------------------------------------------------
8.2	------------------------------------------------------------
4.1_a	------------------------------------------------------------
8.9	------------------------------------------------------------
8.10	------------------------------------------------------------
8.3	TCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCC	1161
8.11	TCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCC	1170
4.2_a	------------------------------------------------------------
4.5	------------------------------------------------------------
4.3	------------------------------------------------------------
4.2_b	------------------------------------------------------------
4.4	------------------------------------------------------------
4.1_b	------------------------------------------------------------
8.1	------------------------------------------------------------
8.7	------------------------------------------------------------
8.6	------------------------------------------------------------
8.8	------------------------------------------------------------
8.5	------------------------------------------------------------
8.2	------------------------------------------------------------
4.1_a	------------------------------------------------------------
8.9	---A--------------------------------------------------------	1052
8.10	---A--------------------------------------------------------	1050
8.3	GCCACCGCGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACTTTGTTAGC	1221
8.11	GC-ACGCGCCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCTTGTTAGC	1229
4.2_a	------------------------------------------------------------
4.5	------------------------------------------------------------
4.3	------------------------------------------------------------
4.2_b	------------------------------------------------------------
4.4	------------------------------------------------------------
4.1_b	------------------------------------------------------------
8.1	------------------------------------------------------------
8.7	------------------------------------------------------------
8.6	------------------------------------------------------------
8.8	------------------------------------------------------------
8.5	------------------------------------------------------------
8.2	------------------------------------------------------------
4.1_a	------------------------------------------------------------
8.9	------------------------------------------------------------
8.10	------------------------------------------------------------
8.3	CAGGATGGTCTCGATCTCCTGACCTCATGATCCACCCGCCTCGGCCTCCCAAAGTGCTGG	1281
8.11	CAGGATGGTCTCGATCTCCTGACCTCATGATCCACCCGCCTCGGCCTCCCAAAGTGCTGG	1289
4.2_a	------------------------------------------------------------
4.5	------------------------------------------------------------
4.3	------------------------------------------------------------
4.2_b	------------------------------------------------------------
4.4	------------------------------------------------------------
4.1_b	------------------------------------------------------------
8.1	------------------------------------------------------------
8.7	------------------------------------------------------------
8.6	------------------------------------------------------------
8.8	------------------------------------------------------------
8.5	------------------------------------------------------------
8.2	------------------------------------------------------------
4.1_a	------------------------------------------------------------
8.9	----------------------------------------------AACTGCCGTCGCAC	1066
8.10	----------------------------------------------AACTGCCGTCGCAC	1064
8.3	GATTACAGGCGTGAGCCACCGCGCCCGGCCGTTTCATGTTTTCTTAAACTGCCATCGCAC	1341
8.11	GATTACAGGCGTGAGCCACCGCGCCCGGCCGTTTCATGTTTTCTTAAACTGCCATCGCAC	1349
4.2_a	----------------------------------------------AACTGCCGTCGCAC	1018
4.5	----------------------------------------------AACTGCCGTCGCAC	1039
4.3	----------------------------------------------AACTGCCGTCGCAC	1041
4.2_b	----------------------------------------------AACTGCCGTCGCAC	1043
4.4	----------------------------------------------AACTGCCGTCGCAC	1042
4.1_b	----------------------------------------------AACTGCCGTCGGAC	1042
8.1	----------------------------------------------AACTGCCGCCGCAC	1041
8.7	----------------------------------------------AACTGCCGCCGCAC	1043
8.6	----------------------------------------------AACTGCCGCCGCAC	1046
8.8	----------------------------------------------AACTGCCGCCGCAC	1046
8.5	----------------------------------------------AACTGCCGCCGCAC	1046
8.2	-------------------------------------ACAAGAGTGAATGGGAAAGACAC	1085
4.1_a	--------------------------------------------TAAACCTTAATCTTTT	1037
	**
8.9	GTCCAAAACCACTCGCTATGCAATGTCTTG-ACCATCTCTCTTTTCTGGCAAATATAAAT	1125
8.10	GTCCGAAACCATTCACTATACAATGTCATT-TTCATCTCTCTTTTCTGGCACACATAAAT	1123
8.3	ATCCGAAATCATTCACTATACAATGTCATG-ACCATCTCTCTTTCCTGGCAAACATAAAT	1400
8.11	ATCCGAAATCATTCACTATACAATGTCATG-ACCATCTCTCTTTTCTGGCAAACATAAAT	1408
4.2_a	GTCCGAAACCGCTCACTATGCAGTGTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT	1077
4.5	GTCCGAAACCGCTCACTATGCAGTCTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT	1098
4.3	GTCCGAAACCGCTCACTATGCAGTGTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT	1100
4.2_b	GTCCGAAACCGCTCACTATGCAGTGTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT	1102
4.4	GTCCGAAACCGCTCACTATGCAGTGTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT	1101
4.1_b	GTCAGAAACTACTCACTATACAATGTCGTG-ACAATCTACATTTTCGGGCAAACACAAAT	1101
8.1	GTCCGAAACCACTCACTATACAATGTCAGG-ACCATCTCTCTTTTCTGGCACACATAAAT	1100
8.7	GTCCGAAACCACTCACTATACAATGTCAGG-ACCATCTCTCTTTTCTGGCACACATAAAT	1102
8.6	GTCCGAAACCACTCACTATACAATGTCAGG-ACCATCTCTCTTTTCTGGTACACATAAAT	1105
8.8	GTCCGAAACCACTCACTATACAATGTCAGG-ACCATCTCTCTTTTCTGGTACACATAAAT	1105
8.5	GTCCGAAACCACTCACTATACAATGTCAGG-ACCATCTCTCTTTTCTGGCACACATAAAT	1105
8.2	AGTGAGAGTGTGTCAAGAGGCAGGGA-ACC-AAGAGCCCTCGGCGCTGA-AGACACAGAC	1142
4.1_a	GCCAAAATATTTGTAGCTTATGTTCCCATTTAACAAGGTTTTCTGGTCAAAACTGTGCAC	1097
	*                                           *       *
8.9	TTTCGGAATGTCATCAATTAGTCTCTCGGTGATTGCATTATTTCCCCAAAGTCTTTTACA	1185
8.10	TTGCGGAATGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTACACA	1183
8.3	TTGGGGATTGTCATCAATTAGTCTCTCAGTGACTGCATGATTTCCACAAAGTCTTTCACA	1460
8.11	TTGGGGATTGTCATCAATTAGTCTCTCGGTGACTGAATGATTTCCACAAAGTCTTTCACA	1468
4.2_a	TTGGGGATTGTCATCAATTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACA	1137
4.5	TTGGGGATTGTCATCAATTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACA	1158
4.3	TTGGGGATTCTCATCAATTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACA	1160
4.2_b	TTGGGGATTGTCATCAATCAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACA	1162
4.4	TTGGGGATTGTCATCAATTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACT	1161
4.1_b	TTGGGGAATGTCATCAAATAGTCTCCCGCTGATTGCATGATT-CCACAAAGTCCTACACA	1160
8.1	TTGGGGAATGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA	1160
8.7	TTGGGGAATGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA	1162
8.6	TTGGGGAAAGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA	1165
8.8	TTGGGGAAAGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA	1165
8.5	TTGGGGAATGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA	1165
8.2	A-GGCGAGCAACG-CAAGGAGAGCTCAAGAGAGACTACCCCTGCCTCCAGG--TACCCGA	1198
4.1_a	CCACATCATTCTAATGAACTTAGTGTCCAATAAAACATGGACTCTCAGTCGTCCCACGGA	1157
	*              *    *      *      *
8.9	GTCTAGTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACGACTTAATGC	1245
8.10	CTCTACATTGTGCACTGAGTATCTCTTCAGACTTTAGTGCATGTTTCTACCACTTGATGC	1243
8.3	GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGT--GTTTCTACCATATGATGC	1518
8.11	GTCTACTTTATGCACTGAGTATCTCTTCAAACTTCAGTGTATGTTTCTACCATTTGATGC	1528
4.2_a	GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACCATTTGATTC	1197
4.5	GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACCATTTCATGC	1218
4.3	GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACCATTTGATTC	1220
4.2_b	GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACCATTTGATGC	1222
4.4	GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACCATTTGATGC	1221
4.1_b	GTCTACATTGTGCACTGAGTATCTCTTCAAACTTCAGTGCTTCTTTCTACCATATGATGC	1220
8.1	GTCTACTTTGTGCAATGAGTATCTCTTCAAACTTCAGTGCATATTTCTACCATTTGATGC	1220
8.7	GCCTACTTTGTGCACTGAGTATCTCTTCAAACTTTAGTGCATGTTTCTACCATTTGATGC	1222
8.6	GTCTACTTTGTGCACTGAGTAACTCTCCAAACTTCAGTGCATGTTTCIACCATTTGATGC	1225
8.8	GTCTACTTTGTGCACTGAGTAACTCTCCAAACTTCAGTGCATGTTTCTACCATTTGATGC	1225
8.5	GCCTACTTTGTGCACTGAGTATCTCTTCAAACTTTAGTGCATGTTTCTACCATTTGATGC	1225
8.2	GTTGGACGAGCACTTGGTGGAAAGAGCCACTCAGGAAAGCAC-CTTAGACCACTGGAAAT	1257
4.1_a	AGTTATTTTGTGTGCATAGTACATCTCTGTGAATATGCCTAATGAGGTATGGAAGGACAC	1217
	* *                           *       *
8.9	TTTATTATTCAGCAATCTAGCTTCCACAAGAGCATTTAATGTAAAGACTTGTCT-TTTTC	1304
8.10	TTTATTACTT-GCCATCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTCTCT-TGTTC	1301
8.3	TTTATTATTTGGCAACCTAGCTTCCAAAAGAGCATTTCATGCAAAGACTTGTCT-TGTTA	1577
8.11	TTTATTATTTGGCAACCTAGCTTCCAAAAGAGCATTTCATGCAAAGACTTGTCT-TGTTA	1587
4.2_a	TTTCTTATTTGGCAATCTAGCTTCCACAAGAGCATTTCACGCAAAGACTTGTCT-TGTTC	1256
4.5	TTTCTTATTTGGCAATCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTGTCT-TGTTC	1277
4.3	TTTCTTATTTGGCAATCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTGTCT-TGTTC	1279
4.2_b	TTTATTATTTGGCAATCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTGTCT-TGTTC	1281
4.4	TTTATTATTTGGCAATCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTGTCT-TCTTC	1280
4.1_b	TTTATCATTTGGCAATCTAGCTTCCACAAGAGCATTTCATGCAAACACTTGTCT-TGTTG	1279
8.1	TTTATTATTTGGCAACCTAGCTTCCACAAGAGCATGTCAGGCAAAGAGTTCTCT-TGTTC	1279
8.7	TTTATTATTTGGCAGCCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTGTCT-TGTTC	1281
8.6	TTTATT---TGGCAGCCTAGCTTCCACAAGAGTATTTCATGCAAAGACTTGTCT-TGTTC	1281
8.8	TTTATT---TGGCAGCCTAGCTTCCACAAGAGTATTTCATGCAAAGACTTGTCT-TGTTC	1281
8.5	TTTATTATTTGGCAGCCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTGTCT-TGTTC	1284
8.2	TCCTCCAAGAGCAAAACAAAACGAAGCCTGAGTTCAACGTCAGAAAACTTGAAGGTACCC	1317
4.1_a	TT-ATTATCCAAACAGAGACATTCCACTGGTGCTAGAGAGCCACAGAC--GGAAGTTTTC	1274
	*                 *          * *            * *        *
8.9	TCCACTGGCAGGTAATTTCACTCGGATATAGAATCATTAGGCTGAACATGGAAAGGTTAT	1364
8.10	TCCACTGGCAGGTAATTTCACTCGGATAGAGAATCAATAGGCTGAACGTGGAAAGGTTAT	1361
8.3	TCCACTGGCAGCTAATTTCATTCGGATAGAGAATCAATAGGCTGAACGTGGAAAGCTTAT	1637
8.11	TCCACTGGCAGCTAATTTCATTCGGATAGAGAATCAATAGGCTGAACGTGGAAAGCTTAT	1647
4.2_a	TCCACTGGCAGGTAATTTCACTCGGACAGAGAATCAATAGGCTCAACGTGGAAAGCTTAT	1316
4.5	TCCACTGGCAGGTAATTTCACTCAGATAGAGAATCAATAGGCTCAACGTGGAAAGCTTAT	1337
4.3	TCCCCTGGCAGGTAATTTCACTCGGACAGAGAATCAATAGGCTCAACGTGGAAAGGTTAT	1339
4.2_b	TCCACTGGCAGGTAATTTCACTCGGACAGAGAATCAATAGGCTCAACGTGGAAAGGTTAT	1341
4.4	TCCACTGGCAGGTAATTTCACTCGGACAGAGAATCAATAGGCTCAACGTGGAAAGGTTAT	1340
4.1_b	TCCACTGGCAAGTAATTCAACACGGATAGAGAATCAATAGGCTCAACGTGGAAAGGTTAT	1339
8.1	TCCACTGGAAGGTAATTTCATTCGCACAGAGAATCAATAGGCTGAACGTAGAAAGGTTAT	1339
8.7	TCCACTGGCCAGTAATTTCACTCGGATAGAGAGTCAATAGGCTGAACGTGGAAAGGTTAT	1341
8.6	TCCACTGGCAGGTAATTTCACTCGGATAGAGAATCAATAGTCTGAACGTGGAAAGGTTAT	1341
8.8	TCCACTGGCAGGTAATTTCACTCGGATAGAGAATCAATAGTCTGAACGTGGAAAGGTTAT	1341
8.5	TCCACTGGCCAGTAATTTCACTCGGATAGAGAGTCAATAGGCTGAACGTGGAAAGGTTAT	1344
8.2	TGCCTCCCAACGTACTTGTGATTCATCAATCAAAATACAAGTGTGGGATGAAAAA-CCAT	1376
4.1_a	TCTGCCTACTGGAAATAAAGC-----CAAGCTTTCTTCTTTCCTCAGCCGTGAGGATTGC	1329
	*            * *           *                        *
8.9	CGCTGGGAGGTCTGTTTGATTCCACGGATCTCTCCTTTTTTATTGAGGAAAAAAATATGC	1424
8.10	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTTATTAAGGAAAAAGATACAC	1421
8.3	CGCTGGAAGGTTTGTTTGTTTCCACGGATCTCTCCTTTCTTATTAGGGAAAAAAATACGC	1697
8.11	CGCTGGAAGGTTTGTTTGTTTCCACGGATCTCTCCTTTCTTATTAGGGAAAAAAATACGC	1707
4.2_a	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTCATTAGGGAAGAAAATACGC	1376
4.5	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTCATTAGGGAAGAAAATACGC	1397
4.3	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTCACTAGGGAAGAAAATACGC	1399
4.2_b	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTCACTAGGGAAGAAAATACGC	1401
4.4	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTCACTAGGGAAGAAAATACGC	1400
4.1_b	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTCATTAGGGAAGAAAATACGC	1399
8.1	CGCTGGAAGCTCTGTTTCATTCCACGGATCTCTCCTTTCTTATTAAAGAAAAAAATACGC	1399
8.7	CCCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTATTATTAAGGAAGAAAATACGC	1401
8.6	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTATTATTAAGGAAAAATATACGC	1401
8.8	CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTATTATTAAGGAAAAATATACGC	1401
8.5	CCCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTATTATTAAGGAAGAAAATACGC	1404
8.2	CATCCTGAA--CAGCAAAGCTCCCTGCTAAACCTCTCTTCGACGAACCCGACAGATCAGG	1434
4.1_a	TGACCTCC--TCTTTATCATTCTCTCTCTCTCTTTTTTTTTAATGAGCCAAGCTCCACCA	1387
	**         *   * *   *
8.9	TGTGCTAATTACTGTACTTCATTGCCTATTCTCAGGTCAGAAAG-----CGCACTTCAGA	1479
8.10	TGCGCTAATTACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTCCGA	1476
8.3	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTCCGA	1752
8.11	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTCCGA	1762
4.2_a	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTTCGT	1431
4.5	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTTCGA	1452
4.3	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTTCGA	1454
4.2_b	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTTCGA	1456
4.4	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTTCGA	1455
4.1_b	TGTGCTAAATATTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CACACTTCCGA	1454
8.1	TGTGCTAAATACCATACTTCATTGACTAATCTCAGGTCAGAAAG-----CACACTTCCGA	1454
8.7	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTCAGA	1456
8.6	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----TGCACTTCAGA	1456
8.8	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----TGCACTTCAGA	1456
8.5	TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG-----CGCACTTCAGA	1459
8.2	AGT-CCATGAACACTGGCACACTCGCTTCTCT---GCAAGGGAG-----GAC-CAGGAGA	1484
4.1_a	AATAATAAGATAAACTTTGTGCAAGACTTGGTAAGAGTAGAGTGTCTGACACCTTATGGT	1447
	*                        *      **   *       *      *
8.9	CTTCT---CCTTCTATCGCTGAAAGGAT-GATGGTATCTGCCAAAAGCACATAC-TCGGA	1534
8.10	CTTCTTGTCCTTCCATCGCTGAGAGGAT-GATGGTATCTGCCAAAAGCACATAT-TTGGA	1534
8.3	CTTCTTCTCTTTCCGTCGCTGAGAGGAT-GATGGTAGCTGCCAAAAGCACATAC-TTGGA	1810
8.11	CTTCTTCTCTTTCCGTCGCTGAGAGGAT-GATGGTAGCTGCCAAAAGCACATAC-TTGGA	1820
4.2_a	CTTCTTGTCCTTCCGTCGCGGAGAGGAT-GATGGCAGCTGCCAAAAGTACATAC-TTGGA	1489
4.5	CTTCTTGTCTTTCCGTCGCTGAGAGGAT-GATGGCAGTTGCCAAAAGTACATAC-TTGGA	1510
4.3	CTTCTTGTCCTTCCGTCGCTGAGAGGAT-GATGGCAGCTGCCAAAAGTACCTAC-TTGGA	1512
4.2_b	CTTCTTGTCCTTCCGTCGCTGAGAGGAT-GATGGCAGCTGCCAAAAGTACCTAC-TTGGA	1514
4.4	CTTCTTGTCCTTCCGTCGCTGAGAGGAT-GATGGCAGCTGCCAAAAGTAACTAC-TTGGA	1513
4.1_b	ATTCTTGTCCTTCGGTCACTGAGAGGAT-GATGGTAGCTGCCAAAAGTACATAC-TTGGA	1512
8.1	TTTCTTGTCCTTCTGTCGCTGAGAGGAT-GATGATAGCTGCCAAAAGTACATAC-TTGGA	1512
8.7	CTTCTTGTCCTTCCGTTGATGAGAGGAT-GACGGTAGCTGCCAAAAGTACATAC-TTGGA	1514
8.6	CTTCTTGTGCTTCCATCGCTGAGAGGAT-GATGGTAGCTGCCAAAAGTACATAC-TTGGA	1514
8.8	CTTCTTGTGCTTCCATCGCTGAGAGGAT-GATGGTAGCTGCCAAAAGTACATAC-TTGGA	1514
8.5	CTTCTTGTCCTTCCGTTGATGAGAGGAT-GACGGTAGCTGCCAAAAGTACATAC-TTGGA	1517
8.2	--------------GCCAAAGGGAAGA---ACAAACACTGCAAGAGGGCTCTGC-TTGTG	1526
4.1_a	GCTATAATACTCAAAGCAAAAGCAAAATCGCCTAGGACCAGAAAAGGGAGTCACATAGGA	1507
	*  *               * * *        * *
8.8	AGT---ACATCCCAGCACAAACACACACACACACA---------------CACGCACACA	1576
8.10	AGT---ACATCCCGGCACAAACACACACACACACA---------------CACACACACA	1576
8.3	GGT---TCATCCCAGCACAAACACACACACACAAA---------------CACACAAACA	1852
8.11	GGT---TCATCCCAGCACAAACACACACACACAAA---------------CACACAAACA	1862
4.2_a	AGT---TCATCCCAGCACAAACACACACACACACGC--CCCCCCCACACACACACACACA	1544
4.5	AGT---TCATCCCAGCACAAACACACACACACACGCGCCCCCCCCACACACACACACACG	1567
4.3	GGT---TCATCCCAGCACAAACACACACACACACACGCCCCCCCC---CACACACACACA	1566
4.2_b	GGT---TCATCCCAGCACAAACACACACACACACACGCCCCCCCC-----CACACACACA	1566
4.4	GGT---TCATCCCAGCACAAACACACACACACACATGCCCCCCCC----ACACACACACA	1566
4.1_b	AGT---TCATCCCAGCACAAACACACATACACACACGCCCCCCCC-----CACACACACA	1564
8.1	AGT---TCATCCCAGCACGAGCACACACACACATAAACACACACA-----CACACACACA	1564
8.7	AGT---TCATCCCAGCACAAGCACACACACACACA--CACAAACA-----CACACACACA	1564
8.6	AGT---TCATCCCAGCACAAGCACACACACACACA--CACAAACA-----CACACACACA	1564
8.8	AGT---TCATCCCAGCACAAGCACACACACACACA--CACAAACA-----CACACACACA	1564
8.5	AGT---TCATCCCAGCACAAGCACACACACACACA--CACAAACA-----CACACACACA	1567
8.2	TGCCAGTGATCTCAGTGGAAGTGCCGACCCACACGTAGGGGAGAA------AAACACACA	1580
4.1_a	AATCTAGAAGACCTATTGGCTGAGAGACCTGCAGCCTCATAGTTCATTAGCTCTC-CATA	1566
	*   *             *    **                     *  *
8.9	AACACACATACTCACAC------------GGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1622
8.10	CACACACACACACACAC------------GGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1622
8.3	CACACACA------CAC------------GGCTTCA--TAGGTAAAGATTTCTTCCCTGA	1892
8.11	CACACACA------CAC------------GGCTTCA--TAGGTAAAGATTTCTTCCCTGA	1902
4.2_a	AACACACTCACACACACACACGCACAC--GGTTTCC--TAGGTAAAGATTTCTTCCCTGC	1600
4.5	AACACAATCACACACACACACTCACAC--GGTTTCC--TACGTAAAGATTTCTTCCCTGC	1623
4.3	AACACACTCACACACACACACCCACAC--GGTTTCC--TAGGTAAAGATTTCTTCCCTGC	1622
4.2_b	AACACACTCACACACACACACGCACAC--GGTTTCC--TAGGTAAAGATTTCTTCCCTGC	1622
4.4	AACACACTCACACACACACACGCACAC--GGTTTCC--TAGGTAAAGATTTCTTCCCTGC	1622
4.1_b	AACACACTCACACACACACACACACACACGGTTTCC--AAGGTAAAGATTTCTTCCCTGC	1622
8.1	CACACACACACACACACAGACACACACAGGGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1622
8.7	CACACACACACAGAGAGAGATACACACACGGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1622
8.6	CACACACACACACACACAGACACACACACGGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1622
8.8	CACACACACACACACACAGACACACACACGGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1622
8.S	CACACACACACAGAGAGAGATACACACACGGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1625
8.2	CACACACACACACACAC------------GGTTTCA--TAGGTAAAGATTTCTTCCCTGA	1626
4.1_a	GCAACTCTCACATGAAATGAAGTCAGTGGTGTTTCAAGTGCTTGAAACCCTCTTTACT-C	1625
	**          *              * ***       * **    ****  **
8.9	CATTCTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACCAT	1679
8.10	CATTGTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT	1679
8.3	CATTGTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCTGGTTACACT	1949
8.11	CATTGTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCTGGTTACACT	1959
4.2_a	CATTGCTTTACCTAAAATAAG---GCAAGTGTGAGGCCACTGTCCCAACCCGGTTACACT	1657
4.5	CATTGCTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT	1680
4.3	CATTGCTTTACCTAAAATAAG---GCAACTGTGAGGCCACTGTCCCAACCCGGTTACACT	1679
4.2_b	CATTGCTTTACCTAAAATAAG---GCAACTGTGAGTCCACTGTCCCAACCCGGTTACACT	1679
4.4	CATTGCTTTACCTAAAATAAG---GCAACTGTGAGGCCACTGTCCCAACCCGGTTACACT	1679
4.1_b	CATTGCTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT	1679
8.1	CATTCTTTTACCTAAAATAAG---GCAACTGTGCGGCCACTGCCCAAACCCGGTTACACT	1679
8.7	CATTCTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT	1679
8.6	CATTCTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT	1679
8.8	CATTCTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT	1679
8.5	CATT-CTGTACCTAAAATAAG---GCAACTGTGTG-CCACTGTCCCAACCCG-TTACACT	1679
8.2	CATTGTTTTACCTAAAATAAG---GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT	1683
4.1_a	TACTTCTAAATGTGAATTAATTATGCAAATTTACTAGCAGTTGCTAGACCT--CAAAAGC	1683
	* *  *  *  * ** ***    **** * *     ** *  *   ***     *
8.9	CATATTATATGTGCCTATCATCCTGAGGAGTAATTT----GATGAAGGTGTTTTAGAAGT	1735
8.10	CATATTACATGTGTCTATCAGCCTGAGGAGTAGTTT----GATTCAGGTGTTCTAGAAGT	1735
8.3	CATATTACATCTGCCTATCATCCTGAGGAGTAATGT----GATTCAGGTGTTCTAGAAGT	2005
8.11	CATATTACATCTGCCTATCATCCTGAGGAGTAATGT----GATTCAGGTGTTCTAGAAGT	2015
4.2_a	CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT----GATTCAGGTGTTCTGGAAGT	1713
4.5	CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT----GATTCAGGTGTTCTGGAAGT	1736
4.3	CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT----GATTCAGGTGTTCTGGAAGT	1735
4.2_b	CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT----GATTCAGGTGTTCTGGAAGT	1735
4.4	CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT----GATTCAGGTGTTCTGGAAGT	1735
4.1_b	CCTATTATATGTGCTTATCATCCTGAGGAGTAATCT----GATTCAGGTGTTCTGGAAGT	1735
8.1	CATATTATATGTGCCTATCACCCTGAGGAGTAATTT----GATTCAGGTGTTCTAGAAGT	1735
8.7	CATATTATATGTGCCTATCACCCTGAGGAGTAATTT----GATTCAGGTGTTCTAGAAGT	1735
8.6	CATATTACATGTGTCTATCAGCCTGAGGAGTAATTT----GATTCAGGTGTTCTAGAAGT	1735
8.8	CATATTACATGTGTCTATCAGCCTGAGGAGTAATTT----GATTCAGGTGTTCTAGAAGT	1735
8.5	CATATTATATGTGCCTATCACCCTGAGGAGTAATTT----GATTCAGGTGTTCTAGAAGT	1735
8.2	CATATTACATGTGTCTATCAGCCTGAGGAGTAGTTT----GATTCAGGTGTTCTAGAAGT	1739
4.1_a	AAAATAATCAGGCATTATTCTACTAAGTATTGGTCTCCATAACTCCTCTATTTTCTTTAG	1743
	** *        ***    ** ** * *  * *     *      * ** *
8.9	CATGATGTGGACTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.10	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.3	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	2065
8.11	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	2075
4.2_a	CATGCTGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCCAGGGGACACACCCTGTGACTC	1773
4.5	CATGCTGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1796
4.3	CATGCTGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCCAGGGGACACACCCTGTGACTC	1795
4.2_b	CATGCTGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCCAGGGGACACACCCTGTGACTC	1795
4.4	CATGCTGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCCAGGGGACACACCCTGTGACTC	1795
4.1_b	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.1	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.7	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.6	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.8	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.5	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1795
8.2	CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC	1799
4.1_a	GAAAAGTTAGTCTAAGACATTTGGCATAAAGGCTATGCCAAAGCTTTGGTGGGGTCAGCC	1803
	*     * * **  * *  ***   *    ** **** *  *          ** *  *
8.9	ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATTGCCCACCTTATGTGCGGGTGGGG	1855
8.10	ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATCGCGCACCTGATGGGTGGGTGGGG	1855
8.3	ATTCCTTAATTAAATGCTGACATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGAGG	2125
8.11	ATTCCTTAATTAAATGCTGATATTTGATTGGTTTATCGCGCACCTGATGAGTGGGTGAGG	2135
4.2_a	CTTCCTGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTAGGTGGGG	1833
4.5	CTTCCTGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGG	1856
4.3	CTTCCTGAATTGAGTGCTGTTATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGG	1855
4.2_b	CTTCCTGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGG	1855
4.4	CTTCCTGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGG	1855
4.1_b	CTTCCTGAATTGAGTGCTGATATTTGATTGGCTAATCGCGCACCTGATGAGTGGGTGGGG	1855
8.1	ATTCCTTAATTGAGTGCTGATATTTGATTGTTTTATCGCGCACCTGATGGGTGGGTGGGG	1855
8.7	ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATCGCACACCTGATGGGTGGGTGGGG	1855
8.6	ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATCGCACACCTGATGGGTGGGTGGGG	1855
8.8	ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATCGCACACCTGATGGGTGGGTGGGG	1855
8.5	ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATCGCACACCTGATGGGTGGGTGGGG	1855
8.2	ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATCGCGCACCTGATGGGTGGGTGGGG	1859
4.1_a	AGGAAGGATTCGTGGGGGTCTCCTTGAAAATACTGCAATA-ATCTAAGAAATCTTCAACC	1862
	* *     *       ****              * ** *
8.9	TGTTCGCTCTTGGTGCGGGTGAGTTATGTAAGGGCTGATTTGGCCAGAGAACTCGTTATT	1915
8.10	TGTTCGCGGTTGGTGGGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	1915
8.3	TGTTCGCCGTTGGTGGGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	2185
8.11	TGTTCGCCGTTGGTGCGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	2195
4.2_a	TGTTCGCGGTTGGTGGGGGTGACTTACAGAAGGGCTGATGTGGCCAGAGAGCTCGTCATT	1893
4.5	TGTTCGCGGTTGGTGGGGTTGACTTACAGAAGGGCTGATGCG-CCAGAGAGCTCGTCATT	1915
4.3	TGTTCGCGGTTCGTGGGGGTGACTTATAGAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	1915
4.2_b	TGTTCGCTGTTGGTGGGGGTGACTTACAGAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	1915
4.4	TGTTCGCGGTTCGTGGGGGTGACTTACAGAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	1915
4.1_b	TGTTCGCGGTTGGTGTGGGTGAGTTATAGAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	1915
8.1	TGTTCGCGGTTGGTGGGGGTGAGTTCTATAAGGGATGATGCGGCCAGAGAGCTCGTCATT	1915
8.7	TGTTCGCGGTTGGTGGGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	1915
8.6	TGTTCGCGGTTGGAGGGGGTGAGTTATATAAGGCCTGATGCGGCCAGAGAGCTGGTCATT	1915
8.8	TGTTCGCGGTTGGAGGGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTGGTCATT	1915
8.5	TGTTCGCCGTTGGTGGGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTCGTCATT	1915
8.2	TGTTCGCGGTTGGTGGGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCCCGTCATT	1919
4.1_a	TATTGCCCCTCAGTACTGTTG-GTCCCCTGTACTTGACTTTTCCCCTTAAGTTTG--ATT	1919
	* **  *  *  *    * **  *              *    **    *    *  ***
8.9	TGAAGACTCTCTCGGAAGAGATAGCGTTTTTCTGCAACCTACGGTCCCAGCAGAAAAACC	1975
8.10	TGAAGACTCTCTCGGAAGAGATAGCATCTTTCTGCAACCTGCGGTCCCAGCCGAAAAACC	1975
8.3	TGAAGACTCTCTCGGAAGAGATAGAGTCTTTCTGCAACATAAGGTCCCAGCCGAAAAACC	2245
8.11	TGAAGACTCTCTCGGAAGAGATAGCGTCTTTCTGCAACATAAGGTCCCAGCCGAAAAACC	2255
4.2_a	TGAAGACTCTCTCGGAAGGGATAGCGTCTTTCTGCAACCTGTGGTCCCAGCAGACAAACC	1953
4.5	TGAAGACTCTCTCGGAAGGGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
4.3	TGAAGACTCTCTCGGAAGGGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
4.2_b	TGAAGACTCTCTCGGAAGGGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
4.4	TGAAGACTCTCTCGGAAGGGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCAGACAAACC	1975
4.1_b	TGAAGACTCTCTCGGAAGAGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
8.1	TGAAGACTCTCTCGGAAGAGATAGCGTCTTGCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
8.7	TGAAGACTCTCTTGGAAGAGATAGCGTCTTGCTGCAACCTGCGGTCCCAGCACAAAAACC	1975
8.6	TGAAGACTCTCTCGGAAGAGATAGCGTCTTGCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
8.8	TGAAGACTCTCTCGGAAGAGATAGCGTCTTGCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
8.5	TGAAGACTCTCTTGGAAGAGATAGCGTCTTGCTGCAACCTGCGGTCCCAGCAGAAAAACC	1975
8.2	TGAAGACTC----GGAAGAGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCCGAAAAACC	1975
4.1_a	CCATTTCCTAATATTATCCTTCCCTCTTCCTCCTCAGCAACTAGTCTTCTAAATTAGAAC	1979
	*   *        *          *    *  ** *     ***         * * *
8.9	TTGTGATCCTTGTTGCGGGCGACATG----------------------------------	2001
8.10	CTGTGATCCTTGTTCCGGGCGACATG----------------------------------	2001
8.3	TTGTGATCCTTGTTCCGGGCGACATG----------------------------------	2271
8.11	TTGTGATCCTTGTTCCGGGCGACATG----------------------------------	2281
4.2_a	TTGTGATCCTTGTTCCAGTCGACATGGAGGACGACTCACTCTACTTGGGAGGTGAGTGGC	2013
4.5	TTGTGATCCTTGTTCCAGTCGACATG----------------------------------	2001
4.3	TTGTGATCCTCGTTCCAGTCGACATG----------------------------------	2001
4.2_b	TTGTGATCCTCGTTCCAGTCGACATG----------------------------------	2001
4.4	TTGTGATCCTTGTTCCAGTCGACATG----------------------------------	2001
4.1_b	TTGTGATCCTTGTTCCAGTCGACATG----------------------------------	2001
8.1	TTGTGATCCTTGTTGCGGGCGACATG----------------------------------	2001
8.7	TTGTGATCCTTGTTGCGGGCGACATG----------------------------------	2001
8.6	TTGTGATCCTTGTTGCGGGCGACATG----------------------------------	2001
8.8	TTGTGATCCTTGTTGCGGGCGACATG----------------------------------	2001
8.5	TTGTGATCCTTGTTGCGGGCGACATG----------------------------------	2001
8.2	TTGTGATCCTTGTTCCGGGCGACATG----------------------------------	2001
4.1_a	TTAAACACAATG----AGCAGATATG----------------------------------	2001
	*     *   *     *  ** ***
8.9	------------------------------------------------------------
8.10	------------------------------------------------------------
8.3	------------------------------------------------------------
8.11	------------------------------------------------------------
4.2_a	AGTTCAACCACTTTTCAAAACTCACATCTTCTCGGCCAGATGCAGCTTTTGCTGAAATCC	2073
4.5	------------------------------------------------------------
4.3	------------------------------------------------------------
4.2_b	------------------------------------------------------------
4.4	------------------------------------------------------------
4.1_b	------------------------------------------------------------
8.1	------------------------------------------------------------
8.7	------------------------------------------------------------
8.6	------------------------------------------------------------
8.8	------------------------------------------------------------
8.5	------------------------------------------------------------
8.2	------------------------------------------------------------
4.1_a	------------------------------------------------------------
8.9	------------------------------------------------------------
8.10	------------------------------------------------------------
8.3	------------------------------------------------------------
8.11	------------------------------------------------------------
4.2_a	AGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATGTGAGACCCGTGTCGACCTCTGAG	2133
4.5	------------------------------------------------------------
4.3	------------------------------------------------------------
4.2_b	------------------------------------------------------------
4.4	------------------------------------------------------------
4.1_b	------------------------------------------------------------
8.1	------------------------------------------------------------
8.7	------------------------------------------------------------
8.6	------------------------------------------------------------
8.8	------------------------------------------------------------
8.5	------------------------------------------------------------
8.2	------------------------------------------------------------
4.1_a	------------------------------------------------------------
8.9	------------------------------------------------------------
8.10	------------------------------------------------------------
8.3	------------------------------------------------------------
8.11	------------------------------------------------------------
4.2_a	ATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGCTTCCTCTGAGTAGCA	2193
4.5	------------------------------------------------------------
4.3	------------------------------------------------------------
4.2_b	------------------------------------------------------------
4.4	------------------------------------------------------------
4.1_b	------------------------------------------------------------
8.1	------------------------------------------------------------
8.7	------------------------------------------------------------
8.6	------------------------------------------------------------
8.8	------------------------------------------------------------
8.5	------------------------------------------------------------
8.2	------------------------------------------------------------
4.1_a	------------------------------------------------------------
8.9	--------------------------------------
8.10	--------------------------------------
8.3	--------------------------------------
8.11	--------------------------------------
4.2_a	GGAGACCTGCTGCGGTGGGGGCTGGGCTCCAGAATATG	2231
4.5	--------------------------------------
4.3	--------------------------------------
4.2_b	--------------------------------------
4.4	--------------------------------------
4.1_b	--------------------------------------
8.1	--------------------------------------
8.7	--------------------------------------
8.6	--------------------------------------
8.8	--------------------------------------
8.5	--------------------------------------
8.2	--------------------------------------
4.1_a	--------------------------------------

HDUB4.6 putative promoter sequence upstream of
initiation ATG
gcatgactgg cagacagctt atcgattggg ctcccctcaa
aatcggttat gagcattcaa gcacaccgat gcccaggtcc
cggctgcagg aataagaccc tccagggtct tgtgtgaagc
ctcggcatct gcattgctca tgcttctggg gatcattctc
ctgaaaatgg tggctccttt ctccctgtgg agcatctttc
taagcagtgc tcttttcttc ccccaggaca ctttacatcc
ggcacaggaa gccttctgat ggagcacacc tggcccatga
aaagacaagg gaaagaaacg gggccaaagg tcacagtcct
ctcatcccat catcctcctt aaaatcatcc taatttcatg
ggccctgaag ccagggctgt ttctttacac ctagaggcct
tggcgccggg cctcaattcc gccctgttcc ttaccgtcta
agacatgttg ggaaaatccc tagagccagg atcttcattc
ctgctaagcc agacagccgg aagacacacc caaattctgt
ccctcttact tcagggaaca tgtccacttt cggcagcatt
acaattttgg caccaaatgt gctaactgca attccaccat
acaatgcgta actggaaatg gaggcaacat ctccgatcct
gaacgatcga tgcgagaatc caggatatgc acggcttatt
ttggcctttt cccactgaaa caagggccag tattaaaaat
ggcacgctat cctctgtttc actccctgct tttaaacgtc
tccgatgttt ctccctgaga cagggcctca cttccgtcag
ccgggctttt ccacggtata attttccttg tttgcttttg
tccaaattag aactttttat ttcacctcta ggaaacgttg
atccattatc acatacgtat ggaaatatta tcacacatgc
tgtgagatac gttgttttta ttttcatcaa ttctttaata
aacaaacggt tatagctggg ataccttctg agttctcaag
ttttttgttt cgtgttttct taaactgccg tcgcacgtcc
gaaaccgctc actatgcagt gtcatgaccg tctctctttt
ctggcaaaca taaatttggg gattgtcatc aattagtctc
tcggggattg catgatttcc ccaaaggctt tcacagtcta
ctttgtgcac tgagtatctc ttcaaacttc agtgcatgtt
tctaccattt catgctttct tatttggcaa tctagcttcc
acaagagcat ttcatgcaaa gacttgtctt gttctccact
ggcaggtaat ttcactcaga tagagaatca ataggctcaa
cgtggaaagg ttatcgctgg aaggtctgtt tgattccacg
gatctctcct ttctcattag ggaagaaaat acgctgtgct
aaatactata cttcattgac tattctcagg tcagaaagcg
cactttcgac ttcttgtctt tccgtcgctg agaggatgat
ggcagctgcc aaaagtacat acttggaagt tcatcgcaga
aaaaacacac acacacacgc gcccccccca cacacacaca
cacgaacaca atcacacaca cacacactca cacggtttcc
tacgtaaaga tttcttccct gccattgctt tacctaaaat
aaggcaactg tgtggccact gtcccaaccc ggttacactc
ctattatatg tgcctatcat cctgaggagt aatttgattc
aggtgttctg gaagtcatgc tgtgggctgt gtctgttgaa
ttcccagcga tgcaagggga cacaccctgt gactccttcc
tgaattgagt gctgatattt gattggctta tcgcgcacct
gatgagtggg tgtggtgttc gcggttggag ggggtgactt
acagaagggc tgatgcggcc agagagctcg tcatttgaag
actctctcgg aagggatagc gtctttctgc aacctgcggt
cccagcagaa aaaccttgtg atccttgttc cagtcgacat
g
HDUB4.7 putative promoter sequence upstream of
initiation ATG
tcctcagcgt cggtttttag gcctggcata agctgtttga
aacccaggaa cgtaccccac ccatcatctt tggcctagtt
aacacctccc ctccgtgtgt ggtggtttgg agaacctgct
ttttcctcat cccactgatc ccaaacccag gacaccctac
agctgctgac caggattaaa cctaatggag atttaatgcc
attaaatcag aagaaattct gattctcagg gactgacatt
cattcactta catacttgca gagtcggcca ggtgtgttgg
ctcacacctg taatcccagc actttgggaa gccgaggtgg
gtggatcacg aggtcaagaa ttcgagacca tcctggccaa
catggtgaaa ccccgtctct actaaaaata caaaaattaa
ctggtgtagc tgtgcgtgcc tgtaatccca gctactcagg
aggctgaggc aggcgatttg cttgaacctg ggaggtggag
gttgcagtga gccaagatta tgccattgca ctccagcctg
ggcaacagag cgagactctc agaaaacaaa aaacccaaaa
acttgcagag tgaatttagg aaaccatgaa gtacacagtt
tgatccaatg ccttcctttt tctctttctc aaatattttg
agccaggtac tatcctagac tgtcttgtga tatttacaat
ctaggagaag gcaggagaga gaactaagaa cagagagcat
gttctgagat gtctgctgtg tttgcaggta ccttccctca
atttccctac tcactggcca tgctggaaag caggtcttgg
cgctatattt ataccatggt acttcccctc cctatactca
attggttggc cagaagccca attgtcactc tctctctctg
tctccctctc gctccctccc tccctccctc cctccctccc
tctccaagat atccagtaac tgactgatca gctgggggtg
ggctctgctg gctgccaaga tgggccacca gcaaaaaggg
aaaattggtt gtgagtgaga agaagagata agaaattcca
cagggctgat aagaaagacc atgggcttcc aggcgcggtg
tttcacgcct gtaatcccag cacttgggag gccaggatgg
tcggatttgg caatctagct tccacaagag catttcacgc
aaagacttgt cttgttctcc actggcaggt aatttcactc
ggatagagaa tcaataggct caacgtggaa aggttatcgc
tggaaggtct gtttaattcc acggatctct cctttctcat
tagggaagaa aatacgctgt gctaaatact atacttcatt
gactattctc aggtcagaaa gcgcactttc gacttcttgt
ccttccgtcg ctgagaggat gatggcagct gccaaaagta
catacttgga agttcatccc agcacaaaca cacacacaca
cacgcccccc cacacacaca cacacaaaca cactcacaca
cacacacgca cacggtttcc taggtaaaga tttcttccct
gccattgctt tacctaaaat aaggcaactg tgaggccact
gtcccaaccc ggttacgctc ctattatatg tgcctatcat
cctgaggagt aatttgattc aggtgttctg gaagtcatgt
tgtgggctgt gtctgttgaa ttcccagcga tgccagggga
cacaccctgt gactccttcc tgaattgagt gctgatattt
gattggctta tcgcgcacct gatgagtggg tggggtgttc
gcggttggtg ggggtgactt acagaagggc tgatgcggcc
agagagctcg tcatttgaag actctctcgg aagggatagc
gtccttctgc aacctgcggt cccagcagac aaaccttgtg
atccttgttc cagtcgacat g
HDUB4.8 putative promoter sequence upstream of
initiation ATG
CAGGGCTCCG TAGAACCACA GAATCTTGGG CGCAACCCTG
CTCAAGCACC CAAATGTGCA TACGAACAGG GTCTCCGTGT
GACGTGTGTG AAAACTACAG TGTGATGAGC ATGACTGGCA
GACAGCTTAT CGATTGGGCT CCCCTCAAAA TCGGTTATGA
GCATTCAAGC ACACCGATGC CCAGGTCCCG GCTGCAGGAA
TAAGACCCTC CAGGGTCTTG TGTGAAGCCT CGGCATCTGC
ATTGCTCATG CTTCTGGGGA TCATTCTCCT GAAAATGGTG
GCTCCTTTCT CCCTGTGGAG CATCTTTCTA AGCAGTGCTC
TTTTCTTCCC CCAGGACACT TTACATCCGG CACAGGAAGC
CTTCTGATGG AGCACACCTG GCCCATGAAA AGACAAGGGA
AAGAAACGGG GCCAAAGGTC ACAGTCCTCT CATCCCATCA
TCCTCCTTAA AATCATCCTA ATTTCATGGG CCCTGAAGCC
AGGGCTGTTT CTTTACACCT AGAGGCCTTG GCGCCGGGCC
TCAATTCCGC CCTGTTCCTT ACCGTCTAAG ACATGTTGGG
AAAATCCCTA GAGCCAGGAT CTTCATTCCT GCTAAGCCAG
ACAGCCGGAA GACACACCCA AATTCTGTCC CTCTTACTTC
AGGGAACATG TCCACTTTCG GCAGCATTAC AATTTTGGCA
CCAAATGTGC TAACTGCAAT TCCACCATAC AATGCGTAAC
TGGAAATGGA GGCAACATCT CCGATCCTGA ACGATCGATG
CGAGAATCCA GGATATGCAC GGCTTATTTT GGCCTTTTCC
CACTGAAACA AGGGCCAGTA TTAAAAATGG CACGCTATCC
TCTGTTTCAC TCCCTGCTTT TAAACGTCTC CGATGTTTCT
CCCTGAGACA GGGCCTCACT TCCGTCAGCC GGGCTTTTCT
ACGGTATAAT TTTCCTTGTT TGCTTTTGTC CAAATTAGAA
CTTTTTATTT CACCTCTAGG AAACGTTGAT CCATTATCAC
ATACGTATGG AAATATTATC ACACATGCTG TGAGATACGT
TGTTTTTATT TTCATCAATT CTTTAATAAA CAAACGGTTA
TAGCTGGGAT ACCTTCTGAG TTCTCAAGTT TTTTGTTTCG
TGTTTTCTTA AACTGCCGTC GCACGTCCGA AACCGCTCAC
TATGCAGTGT CATGACCGTC TCTCTTTTCT GGCAAACATA
AATTTGGGGA TTGTCATCAA TTAGTCTCTC GGGGATTGCA
TGATTTCCCC AAAGGCTTTC ACAGTCTACT TTGTGCACTG
AGTATCTCTT CAAACTTCAG TGCATGTTTC TACCATTTGA
TGCTTTATTA TTTGGCAATC TAGCTTCCAC AAGAGCATTT
CATGCAAAGA CTTGTCTTCT TCTCCACTGG CAGGTAATTT
CACTTGGACA GAGAATCAAT AGGCTCAACG TGGAAAGGTT
ATCGCTGGAA GGTCTGTTTG ATTCCACGGA TCTCTCCTTT
CTCATTAGGG AAGAAAATAC GCTGTGCTAA ATACTATACT
TCATTGACTA TTCTCAGGTC AGAAAGCGCA CTTTCGACTT
CTTGTCCTTC CGTCGCTGAG AGGATGATGG CAGCTGCCAA
AAGTACATAC TTGGAGGTTC ATCCCAGCAC AAACACACAC
ACACACGCGC CCCCCCCACA CACACACACA CGAACACAAT
CACACACACA CACTCACACG GTTTCCTACG TAAAGATTTC
TTCCCTGCCA TTGCTTTACC TAAAATAAGG CAACTGTGTG
GCCACTGTCC CAACCCGGTT ACACTCCTAT TATATGTGCC
TATCATCCTG AGGAGTAATT TGATTCAGGT GTTCTGGAAG
TCATGCTGTG GGCTGTGTCT GTTGAATACC CAGCGATGCA
AGGGGACACA CCCTGTGACT CCTTCCTGAA TTGAGTGCTG
ATATTTGATT GGCTTATCGC GCACCTGATG AGTGGGTGGG
GTGTTCGCGG TTGGTGGGGG TGACTTACAG AAGGGCTGAT
G
HDUB4.9 putative promoter sequence upstream of
initiation ATG
gcatctttct agtcagcgct cttttcttcg cccaggacac
tttacatccg gcacacgaag ccttctgatg gagcacacct
ggcccatgaa aagccaaggg aaagaaacgg ggccaaaggt
cacagtcctc tcctcccatc atcctcctta aaatcatcct
aatttcctgg ccctgaagcc agggctgttt ctttacacct
agaggccttg gcgccgggcc tcaattccgc cctgttcctt
accgtctaag acatgttggg aaaatcccta gagccaggat
cttcattcct gctaagccag acagccggaa gacacaccca
aattctgtcc ctcttacttc agggaacatg tccactttcg
gcagcattac aattttggca ccaaatgtgc taactgcaat
tccaccatac aatgcctaac tggaaatgga ggcaacatct
ccgatcctga acgatcgatg cgagaatcca ggatatgcac
ggcttatttt ggccttttcc cactgaaaca agggccagta
ttaaaaatgg cacgctatcc tctgtttcac tccctgcttt
taaacgtctc cgatgttgct ccctgagaca ggacctcact
tccgtcagcc gggcttttct acggtataat tttccttgtt
tgcttttgtc caaattagaa ctttttattt catctctagg
aaacgttgat ccattatcac atacgtatgg aaatattatc
acacatgctg tgagatacgt tgtttttatt ttcatcaatt
ctttaataaa caaaaggtta tagctgggat accttctgag
ttctcaagtt ttttgtttcg tgttttctta aactgccgtc
gcacgtccga aaccgctcac tatgcagtgt catgaccgtc
tctcttttct ggcaaacata aatttgggga ttgtcatcaa
ttagtctctc ggggattgca tgatttcccc aaaggctttc
acagtctact ttgtgcactg agtatctctt caaacttcag
tgcatgtttc tacaatttga tgctttatta tttggcaatc
tagcttccac aagagcattt catgcaaaga cttgtcttgt
tctccactgg caggtacttt cactcggaca gagaatcaat
aggctcaacg tggaaaggtt ttcgctggaa ggtctgtttg
attccacgga tctctccttt ctcattaggg aagaaaatac
actgtgctaa atactatact tcattgacta ttctcaggtc
agaaagcgca ctttcgactt cttgtccttc cgtcgctgag
aggatgatgg cagctgccaa aagtacatac ttggaagttc
atcccagcac aaacacacac acacgcgccc ccccacacac
acacacaaac acaatcacac acacacacaa tcacacggtt
tcctaggtaa agatttcttc cctgccatgg ctttacctaa
aataaggcaa ctgtgtgacc actgtcccaa cccggttaca
ctcctattat atgtgcctat catcctgagg agtaatttga
ttcaggtgtt ctggaagtca tgctgtggga tgtgtctgtt
gaattcccag cgatgccagg gggacacacc ctgtgactcc
ttcctgaatt gagtgctgat atttgattgg cttatcgcgc
acctgatgag tgggtggggt gttcgcggtt ggtgggggtg
acttacagaa gggctgatgc ggccagagag ctcgtcattt
gaagactctc tcggaaggga tagcgtcttt ctgcaacctg
cggtcccagc agaaaaacct tgtgatcctt gttccagtcg
acatg
HDUB4.10 putative promoter sequence upstream of
initiation ATG
agcaagcttt ggaacagttg gtgaagcccg aagaactcaa
tggagagaat gcctatcatt gtggtgtttg tctccagagg
gcgccggcct ccaagacgtt aactttacac acctctgcca
aggtcctcat ccttgtattg aagagattct ccgatgtcac
aggcaacaag attgccaaga atgtgcaata tcctgagtgc
cttgacatgc agccatacat gtctcagcag aacacaggac
ctcttgtcta tgtcctctat gctgtgctgg tccacgctga
gtggagttgt cacaacggac attacttctc ttatgtcaaa
gctcaagaag gccagtggta taaaatggat gatgccgagg
tcaccgccgc tagcatcact tctgtcctga gtcaacaggc
ctacgtcctc ttttacatcc agaagagtga atgggaaaga
catagtgaga gtgtgtcaag aggcagggaa ccaagagccc
ttggcgcaga agacacagac aggcgagcaa cgcaaggaga
gctcaagaga gaccacccct gcctccaggc ccccgagttg
gacgagcact tggtggaaag agccactcag gaaagcacct
tagaccactg gaaattcctt caagagcaaa acaaaacgaa
gcctgagttc aacgtcagaa aagtcaaagg taccctgcct
cccgacgtac ttgtgattca tcaatcaaaa tacaagtgtg
ggatgaagaa ccatcatcct gaacagcaaa gctccctgct
aaacctctct tcgtcgaccc cgacacatca ggagtccatg
aacactggca cactcgcttc cctgcgaggg agggccagga
gatccaaagg gaagaacaaa cacagcaaga gggctctgct
tgtgtgccag tgatctcagt ggaagtaccg acccacacgt
aggggtgcac acacacacgc acacacacag acacacacat
aactacaccc agaagcgcgc acgcaaacac acacacaccc
acacaaacac gaacaccgtc aatcctacat aaactaatga
ggagcccaag tttctgtctc tacaacaggg acaactggat
agtgatggct acatctcagg atgagcccgc atatgggaaa
catcaagttt tggggtcgtg agtcttccga acctctggag
ggactgtctg agtgtttgtg ttcatgatag gtgacattca
gtgtgtattt ctgaatatga cctaccgacg tgtaggtttg
cgtgtgaggt aattgcaggg gactcggttt cgtattttct
cttggggtgt gtttcattcg tcagttgttg gtcggcatga
gaaggtgaaa tgtggctcat gtgggacatc cgtggatcat
tctcgccacc ttgaatagtg gaaactggaa tgcatttgga
agagaagaac ggtgctcttc tttcttcccc gggctcgccg
tttttacact ggttcctgaa tggacctcag gcgccctggg
acttgtgctc ttgctggaac ccacataacg ccggaagcgg
acagaccgac ttgcctgttt cacggtgccc gcttcccatg
agtccaaacg gaaaattttc ccacgggcat gtaagtcatc
tggaagtaag ctgtattgat aataaaggaa agcaaacaca
ggagtgtgtg tattcaactg aaataaattc agaaagccct
gaaatcaatc tcactgggtg tgtttaaaaa tggcatttgg
ggaatttctg ggtcatttgt ccagctgcga aagctgcatc
tctgaagcac agtccctgtc ccgcagtgag acttattgat
ccgacgtggt gtttccgtgg aaatgattgt gggaaatggc
cccttccttt tctctatttg ctgattagac ttcatggtcc
ctttctcgtc aggtacagtg atcaaagttg accagcccca
gaggaaagct gcccagggca caactcaggg ctccgtagaa
ccacagaatc ttgggcgcaa ccctgctcaa gcacccaaat
g
HDUB4.11 putative promoter sequence upstream of
initiation ATG
cagcaagctt tggaacagtt ggtgaagccc gaagaactca
atggagagaa tgcctatcat tgtggtgttt gtctccagag
ggcgccggcc tccaagacgt taactttaca caactctgcc
aaggtcctca tccttgtatt gaagagattc cccgatgtca
caggcaacaa aattgccaag aatgtgcaat atcctgagtg
ccttgacatg cagccataca tgtctcagca gaacacagga
cctctcgtct atgtcctcta tgctgtgctg gtccacgctg
ggtggagttg tcacaacgga cattactcct cttatgtcaa
agctcaagaa ggccagtggt ataaaatgga tgatgccgag
gtcaccgcct ctagcatcac ttctgtcctg agtcaacagg
cctacgtcct cttttacatc cagaagagtg aatgggaaag
acacagtgag agtgtgtcaa gaggcaggga accaagagcc
cttggcgtag aagacacaga caggcgagca acgcaaggag
agctcaagag agaccacccc tgcctccagg cccccgagtt
ggacgagcac ttggtggaaa gagccactca ggaaagcacc
ttagaccact ggaaattcct tcaagagcaa aacaaaacga
agcctgagtt caacgtcaga agagtcgaag gtacggtgcc
tcccgacgta cttgtgattc atcaatcaaa atacaagtgt
cggatgaaga accatcatcc tgaacagcaa agctccctgc
taaacctctc ttcgacgacc ccgacagatc aggagtccat
gaacactggc acactcgctt ccctacgagg gaggaccagg
agatccaaag ggaagaacaa acacagcaag agggctctgc
ttgtgtgcca gtgatctcag tggaagtacc gacccacacg
taggggtgca tacacacaca cacacacaca cacacacaca
taactacacc cagaagcgcg cacgcaaaca cacacacacc
cacacaaaca cgaacaccgt caatcctaca taaactaatg
aggagcccaa gtttctgtct gtacaacagg gacaactgga
tagagatggc tacatctcag gatgagcccg catatgggaa
acatcaagtt ttggggtcgt gagtcttccg aacctctgga
gggactgtct gagtgtttgt gttcatgata ggtgacattc
agtgtgtatt tatgaatatg acctaccgac gtgtaggttt
gcgtgtgagg taattgcagg ggactcggtt tcgtattttc
tcttggggtg tgtttcattc gacagttgtt ggtcggcacg
agaaggtgaa atttggctca tgtgggacat ccgtggatca
ttctcgccac cttgaatagt ggaaactgga atgcatttgg
aagagaagaa cggtgctctt ctttcttccc cgggctcgcc
gtttttacac tagttcctga atggacctca ggcgccctgg
gacttgtgct cttgctggaa cccacataac gccggaagca
gacagaccga cttgcctgtt tcacggtgcc cgcttcccat
gagtccaaac ggaaaatttt cccacgggca tgtaagtcat
ctggaagtaa gctgtattga taataaagga aagcaaacac
aggagtgtgt gtattcaaca gaaataaatt cagaaagccc
tgaaatcaat ctcactgggt gtgtttaaaa atggcatttg
gggaatttct gggtcatttg tccagctgcg aaagctgcat
ctctgaagca cagtccctgt cccgcagtga gacttattta
tccgacgtgg tgtttccgtg gaaatgattg tgggaaatgg
ccccttcctt ttctctattt gctgactaga cttcatggtc
cctttctcgt caggtacagt gatcaaagtt gaccaacccc
agaggaaagc tgcccagggc acaactcagg gctccataga
accacagaat cttgggagca accctgctca agcacccaaa
tg

Claims

1. An isolated polynucleotide encoding a human deubiquitinating protease selected from the group consisting of hDUB 4.1a, hDUB 4.1b, hDUB 4.2a, hDUB 4.2b, hDUB 4.3, hDUB 4.4, hDUB 4.5, hDUB 4.6, hDUB 4.7, hDUB 4.8, hDUB 4.9, hDUB 4.10, hDUB 4.11, hDUB 8.1, hDUB 8.2, hDUB 8.3, hDUB 8.5, hDUB 8.6, hDUB 8.7, hDUB 8.8, hDUB 8.9, hDUB 8.10, and hDUB 8.11.

2. An isolated polypeptide comprising a human deubiquitinating protease selected from the group consisting of hDUB 4.1a, hDUB 4.2a, hDUB 4.2b, hDUB 4.3, hDUB 4.4, hDUB 4.5, hDUB 4.6, hDUB 4.7, hDUB 4.8, hDUB 4.9, hDUB 4.10, hDUB 4.11, hDUB 8.1, hDUB 8.2, hDUB 8.3, hDUB 8.5, hDUB 8.6, hDUB 8.7, hDUB 8.8, hDUB 8.9, hDUB 8.10, and hDUB 8.11.

3. A method of using a polynucleotide according to claim 1, wherein the polynucleotide is used in an assay to identify an inhibitor of a hDUB of claim 1.

4. A method of using a polypeptide according to claim 2, wherein the polypeptide is used in an assay to identify an inhibitor of a hDUB of claim 2.

5. A method of reducing inflammation by regulating proinflammatory cytokine signaling, by administering a compound capable of inhibiting a polypeptide according to claim 2.

6. A method of modulating an autoimmune disease by altering cytokine receptor signaling involved in lymphocytes proliferation, by administering a compound capable of inhibiting a polypeptide according to claim 2.

7. A method of modulating an immune reaction during infection, by administering a compound capable of inhibiting a polypeptide according to claim 2.

8. A method of reducing inflammation by regulating proinflammatory cytokine signaling, by administering a compound capable of altering regulation of transcription of a polynucleotide of claim 1.

9. A method of modulating an autoimmune disease by altering cytokine receptor signaling involved in lymphocytes proliferation, by administering a compound capable of altering regulation of transcription of a polynucleotide of claim 1.

10. A method of modulating an immune reaction during infection, by administering a compound capable of altering regulation of transcription of a polynucleotide of claim 1.

11. A method of identifying a modulator of a human deubiquitinating protease, wherein a compound is added to the reporter assay comprising a polynucleotide immediately 5′ to a human deubiquitinating protease selected from the group consisting of hDUB 4.1a, hDUB 4.1b, hDUB 4.2a, hDUB 4.2b, hDUB 4.3, hDUB 4.4, hDUB 4.5, hDUB 4.6, hDUB 4.7, hDUB 4.8, hDUB 4.9, hDUB 4.10, hDUB 4.11, hDUB 8.1, hDUB 8.2, hDUB 8.3, hDUB 8.5, hDUB 8.6, hDUB 8.7, hDUB 8.8, hDUB 8.9, hDUB 8.10, and hDUB 8.11 operatively linked to a reporter gene, and the effect of the compound is determined.