Claims
- 1. An isolated polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3; wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
- 2. An isolated polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4; b) a proregion comprising the sequence of SEQ ID No. 5; c) a mature peptide comprising the sequence SEQ ID No. 6; d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6; wherein said signal peptide causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said proregion inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity; wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
- 3. An isolated polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6 and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6 wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
- 4. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide fragment comprising at least 15 consecutive amino acids.
- 5. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide comprising the sequence of SEQ ID No. 3.
- 6. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3.
- 7. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of the polypeptide of SEQ ID No. 3.
- 8. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a signal peptide comprising the sequence of SEQ ID No. 4.
- 9. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a proregion comprising the sequence of SEQ ID No. 5.
- 10. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a mature peptide comprising the sequence of SEQ ID No. 6.
- 11. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6.
- 12. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of the signal peptide comprising the sequence of SEQ ID No. 4.
- 13. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of a proregion comprising the sequence of SEQ ID No. 5.
- 14. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of a mature peptide comprising the sequence of SEQ ID No. 6.
- 15. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising the sequence of SEQ ID No. 6.
- 16. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6.
- 17. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6.
- 18. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising at least 15 consecutive amino acids.
- 19. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising at least 15 consecutive amino acids.
- 20. The isolated polynucleotide according to claim 11, wherein said polynucleotide encodes a polypeptide having an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4.
- 21. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide having an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 5.
- 22. The isolated polynucleotide according to claim 11, wherein said polynucleotide encodes a polypeptide having an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 6.
- 23. A vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3; wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
- 24. A vector comprising a polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4; b) a proregion comprising the sequence of SEQ ID No. 5; c) a mature peptide comprising the sequence SEQ ID No. 6; d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6; wherein said signal peptide causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said proregion inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity; wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
- 25. A vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6 and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6; wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
- 26. The vector according to claim 23, further comprising elements ensuring the expression of said polynucleotide in a host cell.
- 27. The vector according to claim 24, further comprising elements ensuring the expression of said polynucleotide in a host cell.
- 28. The vector according to claim 25, further comprising elements ensuring the expression of said polynucleotide in a host cell.
- 29. A host cell transformed with a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3; wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
- 30. A host cell transformed with a vector comprising a polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4; b) a proregion comprising the sequence of SEQ ID No. 5; c) a mature peptide comprising the sequence SEQ ID No. 6; d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6; wherein said signal peptide causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said proregion inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity; wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
- 31. A host cell comprising a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6; and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6 wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
- 32. A method of producing a polypeptide comprising culturing a host cell transformed with the vector comprising the polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3; wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
- 33. A method of producing a polypeptide comprising culturing a host cell transformed with a vector comprising the polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4; b) a proregion comprising the sequence of SEQ ID No. 5; c) a mature peptide comprising the sequence SEQ ID No. 6; d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6; wherein said signal peptide causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said proregion inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity; wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4; wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule and/or provides a support for the acquisition of the active conformation of the mature peptide and/or is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
- 34. A method of producing a polypeptide comprising culturing a host cell transformed with a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6; b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6 and c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6 wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
- 35. The transformed host cell according to claim 29, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
- 36. The transformed host cell according to claim 30, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
- 37. The transformed host cell according to claim 31, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
- 38. The method according to claim 32, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
- 39. The method according to claim 33, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
- 40. The method according to claim 34, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
Priority Claims (1)
Number |
Date |
Country |
Kind |
97/10823 |
Aug 1997 |
FR |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. Pat. application Ser. No. 09/486,580, filed Aug. 28, 1998; which is a National Stage Application based on International Patent Application No. PCT/FR98/01864, filed Aug. 28, 1998; said PCT/FR98/01864 claims priority to French Patent Application No. FR 97/10823, filed Aug. 29, 1997. The disclosures of each of the above-identified applications are incorporated herein by reference in their entireties, including all figures, tables, and nucleic acid/amino acid sequences.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09486580 |
Feb 2000 |
US |
Child |
10045180 |
Oct 2001 |
US |