Claims
- 1. An isolated polynucleotide comprising a nucleic acid encoding a polypeptide selected from the group consisting of:
- (a) a polypeptide consisting of amino acids 1 to 268 of SEQ ID NO:2;
- (b) a polypeptide consisting of a mino acids 2 to 268 of SEQ ID NO:2;
- (c) a polypeptide consisting of amino acids 16 to 268 in SEQ ID NO:2;
- (d) a polypeptide consisting of amino acids 30 to 268 in SEQ ID NO:2;
- (e) a polypeptide having protease activity consisting of amino acids 30 to 268 of SEQ ID NO:2, except for at least one conservative amino acid substitution;
- (f) a fragment of the polypeptide of (a) having protease activity; and
- (g) a fragment of the polypeptide of (a) which binds to an antibody specific for the polypeptide of (a).
- 2. The isolated polynucleotide of claim 1 wherein said polypeptide is (a).
- 3. The isolated polynucleotide of claim 1 wherein said polypeptide is (b).
- 4. The isolated polynucleotide of claim 1 wherein said polypeptide is (c).
- 5. The isolated polynucleotide of claim 1 wherein said polypeptide is (d).
- 6. The isolated polynucleotide of claim 1 wherein said polypeptide is (e).
- 7. The isolated polynucleotide of claim 1 wherein said polypeptide is (f).
- 8. The isolated polynucleotide of claim 1 wherein said polypeptide is (g).
- 9. An isolated polynucleotide comprising a nucleic acid encoding a polypeptide selected from the group consisting of:
- (a) the full-length polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75751;
- (b) the full-length polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75751, excepting the N-terminal methionine;
- (c) the mature polypeptide encoded by the human cDNA contained in ATCC Deposit No. 75751 ;
- (d) a polypeptide having protease activity consisting of the amino acid sequence of the polypeptide of (c), except for at least one conservative amino acid substitution;
- (e) a fragment of the polypeptide of (a) having protease activity; and
- (f) a fragment of the polypeptide of (a) which binds an antibody specific for the polypeptide of (a).
- 10. The isolated polynucleotide of claim 9 wherein said polypeptide is (a).
- 11. The isolated polynucleotide of claim 9 wherein said polypeptide is (b).
- 12. The isolated polynucleotide of claim 9 wherein said polypeptide is (c).
- 13. The isolated polynucleotide of claim 9 wherein said polypeptide is (d).
- 14. The isolated polynucleotide of claim 9 wherein said polypeptide is (e).
- 15. The isolated polynucleotide of claim 9 wherein said polypeptide is (f).
- 16. An isolated polynucleotide comprising a nucleic acid that hybridizes to a polynucleotide consisting of SEQ ID NO:1, the complement thereof, or The cDNA contained in ATCC Deposit No. 755751 under hybridization conditions comprising hybridization in a wash buffer consisting of 0.5.times.SSC and 0. 1% SDS at 60.degree. C.
- 17. An isolated polynucleotide complementary to the nucleic acid of claim 1.
- 18. An isolated polynucleotide complementary to the nucleic acid of claim 9.
- 19. An isolated polynucleotide complementary to the nucleic acid of claim 16.
- 20. The isolated polynucleotide of claim 1, wherein said nucleic acid is linked to a heterologous polynucleotide.
- 21. The isolated polynucleotide of claim 9, wherein said nucleic acid is linked to a heterologous polynucleotide.
- 22. The isolated polynucleotide of claim 16, wherein said nucleic acid is linked to a heterologous polynucleotide.
- 23. A vector comprising the polynucleotide of claim 1.
- 24. A vector comprising the polynucleotide of claim 9.
- 25. A vector comprising the polynucleotide of claim 16.
- 26. A host cell comprising the polynucleotide of claim 1 operably associated with a heterologous regulatory sequence.
- 27. A host cell comprising the polynucleotide of claim 9 operably associated with a heterologous regulatory sequence.
- 28. A host cell comprising the polynucleotide of claim 16 operably associated with a heterologous regulatory sequence.
- 29. A method of producing a polypeptide comprising:
- (a) culturing the host cell of claim 26 under conditions such that the polypeptide is expressed; and
- b) recovering said polypeptide.
- 30. A method of producing a polypeptide comprising:
- a) culturing the host cell of claim 27 under conditions such that the polypeptide is expressed; and
- (b) recovering said polypeptide.
- 31. A method of screening a ligand comprising:
- (a) obtaining a sample suspected of containing said ligand
- (b) contacting the sample with the host cell of claim 29, and
- (c) determining whether the ligand binds to a polypeptide expressed by the polynucleotide.
- 32. A method of screening a ligand comprising:
- (a) obtaining a sample suspected of containing said ligand,
- (b) contacting the sample with the host cell of claim 30, and
- (c) determining whether the ligand binds to a polypeptide expressed by the polynucleotide.
- 33. A composition comprising the isolated polynucleotide of claim 1.
- 34. A composition comprising the isolated polynucleotide of claim 9.
- 35. A composition comprising the isolated polynucleotide of claim 16.
Parent Case Info
This application is a divisional of application Ser. No. 08/765,192, filed Apr. 24, 1997, now U.S. Pat. No. 5,851,814, which claims priority to PCT/US95/107236, filed Jun. 6, 1995, and which is a CIP of application Ser. No. 08/270,584, filed Jul. 5, 1994, now U.S. Pat. 5,710,035.
US Referenced Citations (7)
Number |
Name |
Date |
Kind |
4968614 |
Takiguchi et al. |
Nov 1990 |
|
4985361 |
Takiguchi et al. |
Jan 1991 |
|
5710035 |
Greene et al. |
Jan 1998 |
|
5738991 |
Braxton et al. |
Apr 1998 |
|
5851814 |
Greene et al. |
Dec 1998 |
|
5856109 |
Braxton et al. |
Jan 1999 |
|
6030791 |
Braxton et al. |
Feb 2000 |
|
Foreign Referenced Citations (5)
Number |
Date |
Country |
157 604 |
Oct 1985 |
EPX |
198 645 |
Oct 1986 |
EPX |
0 768 378 |
Jan 1996 |
EPX |
61-11168 |
May 1986 |
JPX |
9600287 |
Jan 1996 |
WOX |
Non-Patent Literature Citations (7)
Entry |
Largman et al. (1976) Biochem. 15(11):2491-2300. |
Tomomura et al. (1995) J. Biol. Chem. 270(51)30315-30321. |
Supplementary European Search Report, Application No. 95922998.0, dated Mar. 9, 1998. |
Bowie et al. (1990) Science 247:1306-1310. |
Kang et al. (1992) Gene 110(2):181-187. |
Tomomura et al. (1996) FEBS Letts. 386:26-28. |
Yoshino-Yasuda et al. (1998) J. Biochem. 123:45-554. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
765192 |
|
|