Human genes and expression products: II

Abstract

This invention relates to novel human genes, to proteins expressed by the genes, and to variants of the proteins. The invention also relates to diagnostic assays and therapeutic agents related to the genes and proteins, including probes, antisense constructs, and antibodies. The subject nucleic acids have been found to be differentially regulated in tumor cells, particularly in colon cancer tissue.

Description

FIELD OF THE INVENTION

The present invention provides nucleic acid sequences and proteins encoded thereby, as well as probes derived from the nucleic acid sequences, antibodies directed to the encoded proteins, and diagnostic methods for detecting cancerous cells, especially colon cancer cells.

BACKGROUND OF THE INVENTION

Colorectal carcinoma is a malignant neoplastic disease. There is a high incidence of colorectal carcinoma in the Western world, particularly in the United States. Tumors of this type often metastasize through lymphatic and vascular channels. Many patients with colorectal carcinoma eventually die from this disease. In fact, it is estimated that 62,000 persons in the United States alone die of colorectal carcinoma annually.

However, if diagnosed early, colon cancer may be treated effectively by surgical removal of the cancerous tissue. Colorectal cancers originate in the colorectal epithelium and typically are not extensively vascularized (and therefore not invasive) during the early stages of development. Colorectal cancer is thought to result from the clonal expansion of a single mutant cell in the epithelial lining of the colon or rectum. The transition to a highly vascularized, invasive and ultimately metastatic cancer which spreads throughout the body commonly takes ten years or longer. If the cancer is detected prior to invasion, surgical removal of the cancerous tissue is an effective cure. However, colorectal cancer is often detected only upon manifestation of clinical symptoms, such as pain and black tarry stool. Generally, such symptoms are present only when the disease is well established, often after metastasis has occurred, and the prognosis for the patient is poor, even after surgical resection of the cancerous tissue. Early detection of colorectal cancer therefore is important in that detection may significantly reduce its morbidity.

Invasive diagnostic methods such as endoscopic examination allow for direct visual identification, removal, and biopsy of potentially cancerous growths such as polyps. Endoscopy is expensive, uncomfortable, inherently risky, and therefore not a practical tool for screening populations to identify those with colorectal cancer. Non-invasive analysis of stool samples for characteristics indicative of the presence of colorectal cancer or precancer is a preferred alternative for early diagnosis, but no known diagnostic method is available which reliably achieves this goal. A reliable, non-invasive, and accurate technique for diagnosing colon cancer at an early stage would help save many lives.

SUMMARY OF THE INVENTION

The present invention provides nucleic acid sequences and proteins encoded thereby, as well as probes derived from the nucleic acid sequences, antibodies directed to the encoded proteins, and diagnostic methods for detecting cancerous cells, especially colon cancer cells. The sequences disclosed herein have been found to be differentially expressed in samples obtained from colon cancer cell lines and/or colon cancer tissue. The 544 sequences that were obtained were analyzed by “blasting” the sequences against the publicly available databases; based upon the Blast search results it was found that SEQ ID Nos: 1-35 contained novel sequences, SEQ ID Nos: 36-168 contained EST sequences and SEQ ID Nos: 169-544 contained known sequences.

In one aspect, the invention provides an isolated nucleic acid comprising a nucleotide sequence which hybridizes under stringent conditions to a sequence of SEQ ID Nos. 1-544 or a sequence complementary thereto. In a related embodiment, the nucleic acid is at least about 80% or about 100% identical to a sequence corresponding to at least about 12, at least about 15, at least about 25, or at least about 40 consecutive nucleotides up to the full length of one of SEQ ID Nos. 1-544 or a sequence complementary thereto or up to the full length of the gene of which said sequence is a fragment. In certain embodiments, a nucleic acid of the present invention includes at least about five, at least about ten, or at least about twenty nucleic acids from a region designated as novel in Table 2. In certain other embodiments, a nucleic acid of the present invention includes at least about five, at least about ten, or at least about twenty nucleotides which are not included in corresponding clones whose accession numbers are listed in Table 2.

In another aspect, the invention provides an isolated nucleic acid comprising a nucleotide sequence which hybridizes under stringent conditions to a sequence of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto. In a related embodiment, the nucleic acid is at least about 80% or about 100% identical to a sequence corresponding to at least about 12, at least about 15, at least about 25, or at least about 40 consecutive nucleotides up to the full length of one of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35 or a sequence complementary thereto or up to the full length of the gene of which said sequence is a fragment. In certain embodiments, a nucleic acid of the present invention includes at least about five, at least about ten, or at least about twenty nucleic acids from a region designated as novel in Table 2. In certain other embodiments, a nucleic acid of the present invention includes at least about five, at least about ten, or at least about twenty nucleotides which are not included in corresponding clones whose accession numbers are listed in Table 2.

In one embodiment, the invention provides a nucleic acid comprising a nucleotide sequence which hybridizes under stringent conditions to a sequence of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, and a transcriptional regulatory sequence operably linked to the nucleotide sequence to render the nucleotide sequence suitable for use as an expression vector. In another embodiment, the nucleic acid may be included in an expression vector capable of replicating in a prokaryotic or eukaryotic cell. In a related embodiment, the invention provides a host cell transfected with the expression vector.

In another embodiment, the invention provides a transgenic animal having a transgene of a nucleic acid comprising a nucleotide sequence which hybridizes under stringent conditions to a sequence of SEQ ID Nos. 1-168, preferably SEQ ID Nos 1-35, or a sequence complementary thereto incorporated in cells thereof. The transgene modifies the level of expression of the nucleic acid, the stability of a mRNA transcript of the nucleic acid, or the activity of the encoded product of the nucleic acid.

In yet another embodiment, the invention provides substantially pure nucleic acid which hybridizes under stringent conditions to a nucleic acid probe corresponding to at least about 12, at least about 15, at least about 25, or at least about 40 consecutive nucleotides up to the full length of one of SEQ ID Nos. 1-168, preferably SEQ ID Nos 1-35, or a sequence complementary thereto or up to the fill length of the gene of which said sequence is a fragment. The invention also provides an antisense oligonucleotide analog which hybridizes under stringent conditions to at least 12, at least 25, or at least 50 consecutive nucleotides of one of SEQ ID Nos. 1-544 up to the full length of one of SEQ ID Nos. 1-544 or a sequence complementary thereto or up to the full length of the gene of which said sequence is a fragment, and which is resistant to cleavage by a nuclease, preferably an endogenous endonuclease or exonuclease.

In another embodiment, the invention provides a probe/primer comprising a substantially purified oligonucleotide, said oligonucleotide containing a region of nucleotide sequence which hybridizes under stringent conditions to at least about 12, at least about 15, at least about 25, or at least about 40 consecutive nucleotides of sense or antisense sequence selected from SEQ ID Nos. 1-168 up to the full length of one of SEQ ID Nos. 1-168 or a sequence complementary thereto or up to the full length of the gene of which said sequence is a fragment. In preferred embodiments, the probe selectively hybridizes with a target nucleic acid. In another embodiment, the probe may include a label group attached thereto and able to be detected. The label group may be selected from radioisotopes, fluorescent compounds, enzymes, and enzyme co-factors. The invention further provides arrays of at least about 10, at least about 25, at least about 50, or at least about 100 different probes as described above attached to a solid support.

In yet another embodiment, the invention pertains to a method of determining the phenotype of a cell, comprising detecting the differential expression, relative to a normal cell, of at least one nucleic acid which hybridizes under stringent conditions to one of SEQ ID Nos. 1-544, wherein the nucleic acid is differentially expressed by at least a factor of two, at least a factor of five, at least a factor of twenty, or at least a factor of fifty.

In another aspect, the invention provides polypeptides encoded by the subject nucleic acids. In one embodiment, the invention pertains to a polypeptide including an amino acid sequence encoded by a nucleic acid comprising a nucleotide sequence which hybridizes under stringent conditions to a sequence of SEQ ID Nos. 1-168 or a sequence complementary thereto, or a fragment comprising at least about 25, or at least about 40 amino acids thereof. Further provided are antibodies immunoreactive with these polypeptides.

In still another aspect, the invention provides diagnostic methods. In one embodiment, the invention pertains to a method for determining the phenotype of cells from a patient by providing a nucleic acid probe comprising a nucleotide sequence having at least 12, at least about 15, at least about 25, or at least about 40 consecutive nucleotides represented in a sequence of SEQ ID Nos. 1-544 up to the full length of one of SEQ ID Nos. 1-544 or a sequence complementary thereto or up to the full length of the gene of which said sequence is a fragment, obtaining a sample of cells from a patient, providing a second sample of cells substantially all of which are non-cancerous, contacting the nucleic acid probe under stringent conditions with mRNA of each of said first and second cell samples, and comparing (a) the amount of hybridization of the probe with mRNA of the first cell sample, with (b) the amount of hybridization of the probe with mRNA of the second cell sample, wherein a difference of at least a factor of two, at least a factor of five, at least a factor of twenty, or at least a factor of fifty in the amount of hybridization with the mRNA of the first cell sample as compared to the amount of hybridization with the mRNA of the second cell sample is indicative of the phenotype of cells in the first cell sample. Determining the phenotype includes determining the genotype, as the term is used herein.

In another embodiment, the invention provides a test kit for identifying an transformed cells, comprising a probe/primer as described above, for measuring a level of a nucleic acid which hybridizes under stringent conditions to a nucleic acid of SEQ ID Nos. 1-544 in a sample of cells isolated from a patient. In certain embodiments, the kit may further include instructions for using the kit, solutions for suspending or fixing the cells, detectable tags or labels, solutions for rendering a nucleic acid susceptible to hybridization, solutions for lysing cells, or solutions for the purification of nucleic acids.

In another embodiment, the invention provides a method of determining the phenotype of a cell, comprising detecting the differential expression, relative to a normal cell, of at least one protein encoded by a nucleic acid which hybridizes under stringent conditions to one of SEQ ID Nos. 1-544, wherein the protein is differentially expressed by at least a factor of two, at least a factor of five, at least a factor of twenty, or at least a factor of fifty. In one embodiment, the level of the protein is detected in an immunoassay. The invention also pertains to a method for determining the presence or absence of a nucleic acid which hybridizes under stringent conditions to one of SEQ ID Nos. 1-168 in a cell, comprising contacting the cell with a probe as described above. The invention further provides a method for determining the presence or absence of a subject polypeptide encoded by a nucleic acid which hybridizes under stringent conditions to one of SEQ ID Nos. 1-168 in a cell, comprising contacting the cell with an antibody as described above. In yet another embodiment, the invention provides a method for determining the presence of an aberrant mutation (e.g., deletion, insertion, or substitution of nucleic acids) or aberrant methylation in a gene which hybridizes under stringent conditions to a sequence of SEQ ID Nos. 1-168 or a sequence complementary thereto, comprising collecting a sample of cells from a patient, isolating nucleic acid from the cells of the sample, contacting the nucleic acid sample with one or more primers which specifically hybridize to a nucleic acid sequence of SEQ ID Nos. 1-544 under conditions such that hybridization and amplification of the nucleic acid occurs, and comparing the presence, absence, or size of an amplification product to the amplification product of a normal cell.

In one embodiment, the invention provides a test kit for identifying transformed cells, comprising an antibody specific for a protein encoded by a nucleic acid which hybridizes under stringent conditions to any one of SEQ Nos. 1-544. In certain embodiments, the kit further includes instructions for using the kit. In certain embodiments, the kit may further include instructions for using the kit, solutions for suspending or fixing the cells, detectable tags or labels, solutions for rendering a polypeptide susceptible to the binding of an antibody, solutions for lysing cells, or solutions for the purification of polypeptides.

In yet another aspect, the invention provides pharmaceutical compositions including the subject nucleic acids. In one embodiment, an agent which alters the level of expression in a cell of a nucleic acid which hybridizes under stringent conditions to one of SEQ ID Nos. 1-544 or a sequence complementary thereto is identified by providing a cell, treating the cell with a test agent, determining the level of expression in the cell of a nucleic acid which hybridizes under stringent conditions to one of SEQ ID Nos. 1-544 or a sequence complementary thereto, and comparing the level of expression of the nucleic acid in the treated cell with the level of expression of the nucleic acid in an untreated cell, wherein a change in the level of expression of the nucleic acid in the treated cell relative to the level of expression of the nucleic acid in the untreated cell is indicative of an agent which alters the level of expression of the nucleic acid in a cell. The invention further provides a pharmaceutical composition comprising an agent identified by this method. In another embodiment, the invention provides a pharmaceutical composition which includes a polypeptide encoded by a nucleic acid having a nucleotide sequence that hybridizes under stringent conditions to one of SEQ ID Nos. 1-544 or a sequence complementary thereto. In one embodiment, the invention pertains to a pharmaceutical composition comprising a nucleic acid including a sequence which hybridizes under stringent conditions to one of SEQ ID Nos. 1-544 or a sequence complementary thereto.

BRIEF DESCRIPTION OF THE FIGURE

The FIGURE depicts an exemplary assay result for determining differential expression of gene products in cells.

DETAILED DESCRIPTION OF THE INVENTION

The invention relates to nucleic acids having the disclosed nucleotide sequences (SEQ ID Nos. 1-544), as well as full length cDNA, mRNA, and genes corresponding to these sequences, and to polypeptides and proteins encoded by these nucleic acids and genes, and portions thereof.

Also included are polypeptides and proteins encoded by the nucleic acids of SEQ ID Nos. 1-544. The various nucleic acids that can encode these polypeptides and proteins differ because of the degeneracy of the genetic code, in that most amino acids are encoded by more than one triplet codon. The identity of such codons is well known in this art, and this information can be used for the construction of the nucleic acids within the scope of the invention.

Nucleic acids encoding polypeptides and proteins that are variants of the polypeptides and proteins encoded by the nucleic acids and related cDNA and genes are also within the scope of the invention. The variants differ from wild-type protein in having one or more amino acid substitutions that either enhance, add, or diminish a biological activity of the wild-type protein. Once the amino acid change is selected, a nucleic acid encoding that variant is constructed according to the invention.

The following detailed description discloses how to obtain or make full-length cDNA and human genes corresponding to the nucleic acids, how to express these nucleic acids and genes, how to identify structural motifs of the genes, how to identify the function of a protein encoded by a gene corresponding to an nucleic acid, how to use nucleic acids as probes in mapping and in tissue profiling, how to use the corresponding polypeptides and proteins to raise antibodies, and how to use the nucleic acids, polypeptides, and proteins for therapeutic and diagnostic purposes.

The sequences investigated herein have been found to be differentially expressed in samples obtained from colon cancer cell lines and/or colon cancer tissue. However, it is also believed that these sequences may also have utility with other types of cancer. In particular, Table 3 provides nucleic acid sequences which are over-expressed in both cancer cell line SW 480 as well colon cancer tissue obtained from various patients.

Accordingly, certain aspects of the present invention relate to nucleic acids differentially expressed in tumor tissue, especially colon cancer cell lines, polypeptides encoded by such nucleic acids, and antibodies immunoreactive with these polypeptides, and preparations of such compositions. Moreover, the present invention provides diagnostic and therapeutic assays and reagents for detecting and treating disorders involving, for example, aberrant expression of the subject nucleic acids.

I. General

This invention relates in part to novel methods for identifying and/or classifying cancerous cells present in a human tumors, particularly in solid tumors, e.g., carcinomas and sarcomas, such as, for example, breast or colon cancers. The method uses genes that are differentially expressed in cancer cell lines and/or cancer tissue compared with related normal cells, such as normal colon cells, and thereby identifies or classifies tumor cells by the upregulation and/or downregulation of expression of particular genes, an event which is implicated in tumorigenesis.

Upregulation or increased expression of certain genes such as oncogenes, act to promote malignant growth. Downregulation or decreased expression of genes such as tumor suppressor genes also promotes malignant growth. Thus, alteration in the expression of either type of gene is a potential diagnostic indicator for determining whether a subject is at risk of developing or has cancer, e.g., colon cancer.

Accordingly, in one aspect, the invention also provides biomarkers, such as nucleic acid markers, for human tumor cells, e.g., for colon cancer cells. The invention also provides proteins encoded by these nucleic acid markers.

The invention also features methods for identifying drugs useful for treatment of such cancer cells, and for treatment of a cancerous condition, such as colon cancer. Unlike prior methods, the invention provides a means for identifying cancer cells at an early stage of development, so that premalignant cells can be identified prior to their spreading throughout the human body. This allows early detection of potentially cancerous conditions, and treatment of those cancerous conditions prior to spread of the cancerous cells throughout the body, or prior to development of an irreversible cancerous condition.

II. Definitions

For convenience, the meaning of certain terms and phrases used in the specification, examples, and appended claims, are provided below.

The term “an aberrant expression”, as applied to a nucleic acid of the present invention, refers to level of expression of that nucleic acid which differs from the level of expression of that nucleic acid in healthy tissue, or which differs from the activity of the polypeptide present in a healthy subject. An activity of a polypeptide can be aberrant because it is stronger than the activity of its native counterpart. Alternatively, an activity can be aberrant because it is weaker or absent relative to the activity of its native counterpart. An aberrant activity can also be a change in the activity; for example, an aberrant polypeptide can interact with a different target peptide. A cell can have an aberrant expression level of a gene due to overexpression or underexpression of that gene.

The term “agonist”, as used herein, is meant to refer to an agent that mimics or upregulates (e.g., potentiates or supplements) the bioactivity of a protein. An agonist can be a wild-type protein or derivative thereof having at least one bioactivity of the wild-type protein. An agonist can also be a compound that upregulates expression of a gene or which increases at least one bioactivity of a protein. An agonist can also be a compound which increases the interaction of a polypeptide with another molecule, e.g., a target peptide or nucleic acid.

The term “allele”, which is used interchangeably herein with “allelic variant”, refers to alternative forms of a gene or portions thereof. Alleles occupy the same locus or position on homologous chromosomes. When a subject has two identical alleles of a gene, the subject is said to be homozygous for that gene or allele. When a subject has two different alleles of a gene, the subject is said to be heterozygous for the gene. Alleles of a specific gene can differ from each other in a single nucleotide, or several nucleotides, and can include substitutions, deletions, and/or insertions of nucleotides. An allele of a gene can also be a form of a gene containing mutations.

The term “allelic variant of a polymorphic region of a gene” refers to a region of a gene having one of several nucleotide sequences found in that region of the gene in other individuals.

“Antagonist” as used herein is meant to refer to an agent that downregulates (e.g., suppresses or inhibits) at least one bioactivity of a protein. An antagonist can be a compound which inhibits or decreases the interaction between a protein and another molecule, e.g., a target peptide or enzyme substrate. An antagonist can also be a compound that downregulates expression of a gene or which reduces the amount of expressed protein present.

The term “antibody” as used herein is intended to include whole antibodies, e.g., of any isotype (IgG, IgA, IgM, IgE, etc), and includes fragments thereof which are also specifically reactive with a vertebrate, e.g., mammalian, protein. Antibodies can be fragmented using conventional techniques and the fragments screened for utility in the same manner as described above for whole antibodies. Thus, the term includes segments of proteolytically-cleaved or recombinantly-prepared portions of an antibody molecule that are capable of selectively reacting with a certain protein. Nonlimiting examples of such proteolytic and/or recombinant fragments include Fab, F(ab′)2, Fab′, Fv, and single chain antibodies (scFv) containing a V[L] and/or V[H] domain joined by a peptide linker. The scFv's may be covalently or non-covalently linked to form antibodies having two or more binding sites. The subject invention includes polyclonal, monoclonal, or other purified preparations of antibodies and recombinant antibodies.

The phenomenon of “apoptosis” is well known, and can be described as a programmed death of cells. As is known, apoptosis is contrasted with “necrosis”, a phenomenon when cells die as a result of being killed by a toxic material, or other external effect. Apoptosis involves chromatic condensation, membrane blebbing, and fragmentation of DNA, all of which are generally visible upon microscopic examination.

A disease, disorder, or condition “associated with” or “characterized by” an aberrant expression of a nucleic acid refers to a disease, disorder, or condition in a subject which is caused by, contributed to by, or causative of an aberrant level of expression of a nucleic acid.

As used herein the term “bioactive fragment of a polypeptide” refers to a fragment of a full-length polypeptide, wherein the fragment specifically agonizes (mimics) or antagonizes (inhibits) the activity of a wild-type polypeptide. The bioactive fragment preferably is a fragment capable of interacting with at least one other molecule, e.g., protein, small molecule, or DNA, which a full length protein can bind.

“Biological activity” or “bioactivity” or “activity” or “biological function”, which are used interchangeably, herein mean an effector or antigenic function that is directly or indirectly performed by a polypeptide (whether in its native or denatured conformation), or by any subsequence thereof. Biological activities include binding to polypeptides, binding to other proteins or molecules, activity as a DNA binding protein, as a transcription regulator, ability to bind damaged DNA, etc. A bioactivity can be modulated by directly affecting the subject polypeptide. Alternatively, a bioactivity can be altered by modulating the level of the polypeptide, such as by modulating expression of the corresponding gene.

The term “biomarker” refers a biological molecule, e.g., a nucleic acid, peptide, hormone, etc., whose presence or concentration can be detected and correlated with a known condition, such as a disease state.

“Cells,” “host cells”, or “recombinant host cells” are terms used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

A “chimeric polypeptide” or “fusion polypeptide” is a fusion of a first amino acid sequence encoding one of the subject polypeptides with a second amino acid sequence defining a domain (e.g., polypeptide portion) foreign to and not substantially homologous with any domain of the subject polypeptide. A chimeric polypeptide may present a foreign domain which is found (albeit in a different polypeptide) in an organism which also expresses the first polypeptide, or it may be an “interspecies,” “intergenic,” etc., fusion of polypeptide structures expressed by different kinds of organisms. In general, a fusion polypeptide can be represented by the general formula (X) n -(Y) m -(Z) n , wherein Y represents a portion of the subject polypeptide, and X and Z are each independently absent or represent amino acid sequences which are not related to the native sequence found in an organism, or which are not found as a polypeptide chain contiguous with the subject sequence, where m is an integer greater than or equal to one, and each occurrence of n is, independently, 0 or an integer greater than or equal to 1 (n and m are preferably no greater than 5 or 10).

A “delivery complex” shall mean a targeting means (e.g., a molecule that results in higher affinity binding of a nucleic acid, protein, polypeptide or peptide to a target cell surface and/or increased cellular or nuclear uptake by a target cell). Examples of targeting means include: sterols (e.g., cholesterol), lipids (e.g., a cationic lipid, virosome or liposome), viruses (e.g., adenovirus, adeno-associated virus, and retrovirus), or target cell-specific binding agents (e.g., ligands recognized by target cell specific receptors). Preferred complexes are sufficiently stable in vivo to prevent significant uncoupling prior to internalization by the target cell. However, the complex is cleavable under appropriate conditions within the cell so that the nucleic acid, protein, polypeptide or peptide is released in a functional form.

As is well known, genes or a particular polypeptide may exist in single or multiple copies within the genome of an individual. Such duplicate genes may be identical or may have certain modifications, including nucleotide substitutions, additions or deletions, which all still code for polypeptides having substantially the same activity. The term “DNA sequence encoding a polypeptide” may thus refer to one or more genes within a particular individual. Moreover, certain differences in nucleotide sequences may exist between individual organisms, which are called aalleles. Such allelic differences may or may not result in differences in amino acid sequence of the encoded polypeptide yet still encode a polypeptide with the same biological activity.

The term “equivalent” is understood to include nucleotide sequences encoding functionally equivalent polypeptides. Equivalent nucleotide sequences will include sequences that differ by one or more nucleotide substitutions, additions or deletions, such as allelic variants; and will, therefore, include sequences that differ from the nucleotide sequence of the nucleic acids shown in SEQ ID NOs: 1-544 due to the degeneracy of the genetic code.

As used herein, the terms “gene”, “recombinant gene”, and “gene construct” refer to a nucleic acid of the present invention associated with an open reading frame, including both exon and (optionally) intron sequences.

A “recombinant gene” refers to nucleic acid encoding a polypeptide and comprising exon sequences, though it may optionally include intron sequences which are derived from, for example, a related or unrelated chromosomal gene. The term “intron” refers to a DNA sequence present in a given gene which is not translated into protein and is generally found between exons.

The term “growth” or “growth state” of a cell refers to the proliferative state of a cell as well as to its differentiative state. Accordingly, the term refers to the phase of the cell cycle in which the cell is, e.g., G0, G1, G2, prophase, metaphase, or telophase, as well as to its state of differentiation, e.g., undifferentiated, partially differentiated, or fully differentiated. Without wanting to be limited, differentiation of a cell is usually accompanied by a decrease in the proliferative rate of a cell.

“Homology” or “identity” or “similarity” refers to sequence similarity between two peptides or between two nucleic acid molecules, with identity being a more strict comparison. Homology and identity can each be determined by comparing a position in each sequence which may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same base or amino acid, then the molecules are identical at that position. A degree of homology or similarity or identity between nucleic acid sequences is a function of the number of identical or matching nucleotides at positions shared by the nucleic acid sequences. A degree of identity of amino acid sequences is a function of the number of identical amino acids at positions shared by the amino acid sequences. A degree of homology or similarity of amino acid sequences is a function of the number of amino acids, i.e., structurally related, at positions shared by the amino acid sequences. An “unrelated” or “non-homologous” sequence shares less than 40% identity, though preferably less than 25% identity, with one of the sequences of the present invention.

The term “percent identical” refers to sequence identity between two amino acid sequences or between two nucleotide sequences. Identity can each be determined by comparing a position in each sequence which may be aligned for purposes of comparison. When an equivalent position in the compared sequences is occupied by the same base or amino acid, then the molecules are identical at that position; when the equivalent site occupied by the same or a similar amino acid residue (e.g., similar in steric and/or electronic nature), then the molecules can be referred to as homologous (similar) at that position. Expression as a percentage of homology, similarity, or identity refers to a function of the number of identical or similar amino acids at positions shared by the compared sequences. Various alignment algorithms and/or programs may be used, including FASTA, BLAST, or ENTREZ. FASTA and BLAST are available as a part of the GCG sequence analysis package (University of Wisconsin, Madison, Wis.), and can be used with, e.g., default settings. ENTREZ is available through the National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Md. In one embodiment, the percent identity of two sequences can be determined by the GCG program with a gap weight of 1, e.g., each amino acid gap is weighted as if it were a single amino acid or nucleotide mismatch between the two sequences.

Other techniques for alignment are described in Methods in Enzymology , vol. 266: Computer Methods for Macromolecular Sequence Analysis (1996), ed. Doolittle, Academic Press, Inc., a division of Harcourt Brace & Co., San Diego, Calif., USA. Preferably, an alignment program that permits gaps in the sequence is utilized to align the sequences. The Smith-Waterman is one type of algorithm that permits gaps in sequence alignments. See Meth. Mol. Biol. 70: 173-187 (1997). Also, the GAP program using the Needleman and Wunsch alignment method can be utilized to align sequences. An alternative search strategy uses MPSRCH software, which runs on a MASPAR computer. MPSRCH uses a Smith-Waterman algorithm to score sequences on a massively parallel computer. This approach improves ability to pick up distantly related matches, and is especially tolerant of small gaps and nucleotide sequence errors. Nucleic acid-encoded amino acid sequences can be used to search both protein and DNA databases.

Databases with individual sequences are described in Methods in Enzymology , ed. Doolittle, supra. Databases include Genbank, EMBL, and DNA Database of Japan (DDBJ).

Preferred nucleic acids have a sequence at least 70%, and more preferably 80% identical and more preferably 90% and even more preferably at least 95% identical to an nucleic acid sequence of a sequence shown in one of SEQ ID NOS: 1-544. Nucleic acids at least 90%, more preferably 95%, and most preferably at least about 98-99% identical with a nucleic sequence represented in one of SEQ ID NOS: 1-544 are of course also within the scope of the invention. In preferred embodiments, the nucleic acid is mammalian.

The term “interact” as used herein is meant to include detectable interactions (e.g., biochemical interactions) between molecules, such as interaction between protein-protein, protein-nucleic acid, nucleic acid-nucleic acid, and protein-small molecule or nucleic acid-small molecule in nature.

The term “isolated” as used herein with respect to nucleic acids, such as DNA or RNA, refers to molecules separated from other DNAs, or RNAs, respectively, that are present in the natural source of the macromolecule. The term isolated as used herein also refers to a nucleic acid or peptide that is substantially free of cellular material, viral material, or culture medium when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. Moreover, an “isolated nucleic acid” is meant to include nucleic acid fragments which are not naturally occurring as fragments and would not be found in the natural state. The term “isolated” is also used herein to refer to polypeptides which are isolated from other cellular proteins and is meant to encompass both purified and recombinant polypeptides.

The terms “modulated” and “differentially regulated” as used herein refer to both upregulation (i.e., activation or stimulation (e.g., by agonizing or potentiating)) and downregulation (i.e., inhibition or suppression (e.g., by antagonizing, decreasing or inhibiting)).

The term “mutated gene” refers to an allelic form of a gene, which is capable of altering the phenotype of a subject having the mutated gene relative to a subject which does not have the mutated gene. If a subject must be homozygous for this mutation to have an altered phenotype, the mutation is said to be recessive. If one copy of the mutated gene is sufficient to alter the genotype of the subject, the mutation is said to be dominant. If a subject has one copy of the mutated gene and has a phenotype that is intermediate between that of a homozygous and that of a heterozygous subject (for that gene), the mutation is said to be co-dominant.

The designation “N”, where it appears in the accompanying Sequence Listing, indicates that the identity of the corresponding nucleotide is unknown. “N” should therefore not necessarily be interpreted as permitting substitution with any nucleotide, e.g., A, T, C, or G, but rather as holding the place of a nucleotide whose identity has not been conclusively determined.

The “non-human animals” of the invention include mammalians such as rodents, non-human primates, sheep, dog, cow, chickens, amphibians, reptiles, etc. Preferred non-human animals are selected from the rodent family including rat and mouse, most preferably mouse, though transgenic amphibians, such as members of the Xenopus genus, and transgenic chickens can also provide important tools for understanding and identifying agents which can affect, for example, embryogenesis and tissue formation. The term “chimeric animal” is used herein to refer to animals in which the recombinant gene is found, or in which the recombinant gene is expressed in some but not all cells of the animal. The term “tissue-specific chimeric animal” indicates that one of the recombinant genes is present and/or expressed or disrupted in some tissues but not others.

As used herein, the term “nucleic acid” refers to polynucleotides such as deoxyribonucleic acid (DNA), and, where appropriate, ribonucleic acid (RNA). The term should also be understood to include, as equivalents, analogs of either RNA or DNA made from nucleotide analogs, and, as applicable to the embodiment being described, single (sense or antisense) and double-stranded polynucleotides. ESTs, chromosomes, cDNAs, mRNAs, and rRNAs are representative examples of molecules that may be referred to as nucleic acids.

The term “nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO. x” refers to the nucleotide sequence of the complementary strand of a nucleic acid strand having SEQ ID NO. x. The term “complementary strand” is used herein interchangeably with the term “complement”. The complement of a nucleic acid strand can be the complement of a coding strand or the complement of a non-coding strand.

The term “polymorphism” refers to the coexistence of more than one form of a gene or portion (e.g., allelic variant) thereof. A portion of a gene of which there are at least two different forms, i.e., two different nucleotide sequences, is referred to as a “polymorphic region of a gene”. A polymorphic region can be a single nucleotide, the identity of which differs in different alleles. A polymorphic region can also be several nucleotides long.

A “polymorphic gene” refers to a gene having at least one polymorphic region.

As used herein, the term “promoter” means a DNA sequence that regulates expression of a selected DNA sequence operably linked to the promoter, and which effects expression of the selected DNA sequence in cells. The term encompasses “tissue specific” promoters, i.e., promoters which effect expression of the selected DNA sequence only in specific cells (e.g., cells of a specific tissue). The term also covers so-called “leaky” promoters, which regulate expression of a selected DNA primarily in one tissue, but cause expression in other tissues as well. The term also encompasses non-tissue specific promoters and promoters that constitutively expressed or that are inducible (i.e., expression levels can be controlled).

The terms “protein”, “polypeptide”, and “peptide” are used interchangeably herein when referring to a gene product.

The term “recombinant protein” refers to a polypeptide of the present invention which is produced by recombinant DNA techniques, wherein generally, DNA encoding a polypeptide is inserted into a suitable expression vector which is in turn used to transform a host cell to produce the heterologous protein. Moreover, the phrase “derived from”, with respect to a recombinant gene, is meant to include within the meaning of “recombinant protein” those proteins having an amino acid sequence of a native polypeptide, or an amino acid sequence similar thereto which is generated by mutations including substitutions and deletions (including truncation) of a naturally occurring form of the polypeptide.

“Small molecule” as used herein, is meant to refer to a composition, which has a molecular weight of less than about 5 kD and most preferably less than about 4 kD. Small molecules can be nucleic acids, peptides, polypeptides, peptidomimetics, carbohydrates, lipids or other organic (carbon-containing) or inorganic molecules. Many pharmaceutical companies have extensive libraries of chemical and/or biological mixtures, often fungal, bacterial, or algal extracts, which can be screened with any of the assays of the invention to identify compounds that modulate a bioactivity.

As used herein, the term “specifically hybridizes” or “specifically detects” refers to the ability of a nucleic acid molecule of the invention to hybridize to at least a portion of, for example; approximately 6, 12, 15, 20, 30, 50, 100, 150, 200, 300, 350, 400, 500, 750, or 1000 contiguous nucleotides of a nucleic acid designated in any one of SEQ ID Nos: 1-544, or a sequence complementary thereto, or naturally occurring mutants thereof, such that it has less than 15%, preferably less than 10%, and more preferably less than 5% background hybridization to a cellular nucleic acid (e.g., mRNA or genomic DNA) encoding a different protein. In preferred embodiments, the oligonucleotide probe detects only a specific nucleic acid, e.g., it does not substantially hybridize to similar or related nucleic acids, or complements thereof.

“Transcriptional regulatory sequence” is a generic term used throughout the specification to refer to DNA sequences, such as initiation signals, enhancers, and promoters, which induce or control transcription of protein coding sequences with which they are operably linked. In preferred embodiments, transcription of one of the genes is under the control of a promoter sequence (or other transcriptional regulatory sequence) which controls the expression of the recombinant gene in a cell-type in which expression is intended. It will also be understood that the recombinant gene can be under the control of transcriptional regulatory sequences which are the same or which are different from those sequences which control transcription of the naturally-occurring forms of the polypeptide.

As used herein, the term “transfection” means the introduction of a nucleic acid, e.g., via an expression vector, into a recipient cell by nucleic acid-mediated gene transfer. “Transformation”, as used herein, refers to a process in which a cell's genotype is changed as a result of the cellular uptake of exogenous DNA or RNA, and, for example, the transformed cell expresses a recombinant form of a polypeptide or, in the case of anti-sense expression from the transferred gene, the expression of the target gene is disrupted.

As used herein, the term “transgene” means a nucleic acid sequence (or an antisense transcript thereto) which has been introduced into a cell. A transgene could be partly or entirely heterologous, i.e., foreign, to the transgenic animal or cell into which it is introduced, or, is homologous to an endogenous gene of the transgenic animal or cell into which it is introduced, but which is designed to be inserted, or is inserted, into the animal's genome in such a way as to alter the genome of the cell into which it is inserted (e.g., it is inserted at a location which differs from that of the natural gene or its insertion results in a knockout). A transgene can also be present in a cell in the form of an episome. A transgene can include one or more transcriptional regulatory sequences and any other nucleic acid, such as introns, that may be necessary for optimal expression of a selected nucleic acid.

A “transgenic animal” refers to any animal, preferably a non-human mammal, bird or an amphibian, in which one or more of the cells of the animal contain heterologous nucleic acid introduced by way of human intervention, such as by transgenic techniques well known in the art. The nucleic acid is introduced into the cell, directly or indirectly by introduction into a precursor of the cell, by way of deliberate genetic manipulation, such as by microinjection or by infection with a recombinant virus. The term genetic manipulation does not include classical cross-breeding, or in vitro fertilization, but rather is directed to the introduction of a recombinant DNA molecule. This molecule may be integrated within a chromosome, or it may be extra-chromosomally replicating DNA. In the typical transgenic animals described herein, the transgene causes cells to express a recombinant form of one of the subject polypeptide, e.g. either agonistic or antagonistic forms. However, transgenic animals in which the recombinant gene is silent are also contemplated, as for example, the FLP or CRE recombinase dependent constructs described below. Moreover, “transgenic animal” also includes those recombinant animals in which gene disruption of one or more genes is caused by human intervention, including both recombination and antisense techniques.

The term “treating” as used herein is intended to encompass curing as well as ameliorating at least one symptom of the condition or disease.

The term “vector” refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of preferred vector is an episome, i.e., a nucleic acid capable of extra-chromosomal replication. Preferred vectors are those capable of autonomous replication and/or expression of nucleic acids to which they are linked. Vectors capable of directing the expression of genes to which they are operatively linked are referred to herein as “expression vectors”. In general, expression vectors of utility in recombinant DNA techniques are often in the form of “plasmids” which refer generally to circular double stranded DNA loops which, in their vector form are not bound to the chromosome. In the present specification, “plasmid” and “vector” are used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors which serve equivalent functions and which become known in the art subsequently hereto.

The term “wild-type allele” refers to an allele of a gene which, when present in two copies in a subject results in a wild-type phenotype. There can be several different wild-type alleles of a specific gene, since certain nucleotide changes in a gene may not affect the phenotype of a subject having two copies of the gene with the nucleotide changes.

III. Nucleic Acids of the Present Invention

As described below, one aspect of the invention pertains to isolated nucleic acids, variants, and/or equivalents of such nucleic acids.

Nucleic acids of the present invention have been identified as differentially expressed in tumor cells, e.g., colon cancer-derived cell lines (relative to the expression levels in normal tissue, e.g., normal colon tissue and/or normal non-colon tissue), such as SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto. In certain embodiments, the subject nucleic acids are differentially expressed by at least a factor of two, preferably at least a factor of five, even more preferably at least a factor of twenty, still more preferably at least a factor of fifty. Preferred nucleic acids include sequences identified as differentially expressed both in colon cancer cell tissue and colon cancer cell lines. In preferred embodiments, nucleic acids of the present invention are upregulated in tumor cells, especially colon cancer tissue and/or colon cancer-derived cell lines. In another embodiment, nucleic acids of the present invention are downregulated in tumor cells, especially colon cancer tissue and/or colon cancer-derived cell lines.

Table 1 indicates those sequences which are over- or underexpressed in a colon cancer-derived cell line relative to normal tissue, and further designates those sequences which are also differentially regulated in colon cancer tissue. The designation O indicates that the corresponding sequence was overexpressed, M indicates possible overexpression, N indicates no differential expression, and U indicates underexpression.

Genes which are upregulated, such as oncogenes, or downregulated, such as tumor suppressors, in aberrantly proliferating cells may be targets for diagnostic or therapeutic techniques. For example, upregulation of the cdc2 gene induces mitosis. Overexpression of the myt1 gene, a mitotic deactivator, negatively regulates the activity of cdc2. Aberrant proliferation may thus be induced either by upregulating cdc2 or by downregulating myt1. Similarly, downregulation of tumor suppressors such as p53 and Rb have been implicated in tumorigenesis.

Particularly preferred polypeptides are those that are encoded by nucleic acid sequences at least about 70%, 75%, 80%, 90%, 95%, 97%, or 98% similar to a nucleic acid sequence of SEQ ID Nos. 1-544. Preferably, the nucleic acid includes all or a portion (e.g., at least about 12, at least about 15, at least about 25, or at least about 40 nucleotides) of the nucleotide sequence corresponding to the nucleic acid of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto.

Still other preferred nucleic acids of the present invention encode a polypeptide comprising at least a portion of a polypeptide encoded by one of SEQ ID Nos. 1-544. For example, preferred nucleic acid molecules for use as probes/primers or antisense molecules (i.e., noncoding nucleic acid molecules) can comprise at least about 12, 20, 30, 50, 60, 70, 80, 90, or 100 base pairs in length up to the length of the complete gene. Coding nucleic acid molecules can comprise, for example, from about 50, 60, 70, 80, 90, or 100 base pairs up to the length of the complete gene.

Another aspect of the invention provides a nucleic acid which hybridizes under low, medium, or high stringency conditions to a nucleic acid sequence represented by one of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto. Appropriate stringency conditions which promote DNA hybridization, for example, 6.0×sodium chloride/sodium citrate (SSC) at about 45° C., followed by a wash of 2.0×SSC at 50° C., are known to those skilled in the art or can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-12.3.6. For example, the salt concentration in the wash step can be selected from a low stringency of about 2.0×SSC at 50° C. to a high stringency of about 0.2×SSC at 50° C. In addition, the temperature in the wash step can be increased from low stringency conditions at room temperature, about 22° C., to high stringency conditions at about 65° C. Both temperature and salt may be varied, or temperature or salt concentration may be held constant while the other variable is changed. In a preferred embodiment, a nucleic acid of the present invention will bind to one of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, under moderately stringent conditions, for example at about 2.0×SSC and about 40° C. In a particularly preferred embodiment, a nucleic acid of the present invention will bind to one of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, under high stringency conditions.

In one embodiment, the invention provides nucleic acids which hybridize under low stringency conditions of 6×SSC at room temperature followed by a wash at 2×SSC at room temperature.

In another embodiment, the invention provides nucleic acids which hybridize under high stringency conditions of 2×SSC at 65° C. followed by a wash at 0.2×SSC at 65° C.

Nucleic acids having a sequence that differs from the nucleotide sequences shown in one of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, due to degeneracy in the genetic code, are also within the scope of the invention. Such nucleic acids encode functionally equivalent peptides (i.e., a peptide having equivalent or similar biological activity) but differ in sequence from the sequence shown in the sequence listing due to degeneracy in the genetic code. For example, a number of amino acids are designated by more than one triplet. Codons that specify the same amino acid, or synonyms (for example, CAU and CAC each encode histidine) may result in “silent” mutations which do not affect the amino acid sequence of a polypeptide. However, it is expected that DNA sequence polymorphisms that do lead to changes in the amino acid sequences of the subject polypeptides will exist among mammals. One skilled in the art will appreciate that these variations in one or more nucleotides (e.g., up to about 3-5% of the nucleotides) of the nucleic acids encoding polypeptides having an activity of a polypeptide may exist among individuals of a given species due to natural allelic variation.

Also within the scope of the invention are nucleic acids encoding splicing variants of proteins encoded by a nucleic acid of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, or natural homologs of such proteins. Such homologs can be cloned by hybridization or PCR, as further described herein.

The polynucleotide sequence may also encode for a leader sequence, e.g., the natural leader sequence or a heterologous leader sequence, for a subject polypeptide. For example, the desired DNA sequence may be fused in the same reading frame to a DNA sequence which aids in expression and secretion of the polypeptide from the host cell, for example, a leader sequence which functions as a secretory sequence for controlling transport of the polypeptide from the cell. The protein having a leader sequence is a preprotein and may have the leader sequence cleaved by the host cell to form the mature form of the protein.

The polynucleotide of the present invention may also be fused in frame to a marker sequence, also referred to herein as “Tag sequence” encoding a “Tag peptide”, which allows for marking and/or purification of the polypeptide of the present invention. In a preferred embodiment, the marker sequence is a hexahistidine tag, e.g., supplied by a PQE-9 vector. Numerous other Tag peptides are available commercially. Other frequently used Tags include myc-epitopes (e.g., see Ellison et al. (1991) J Biol Chem 266:21150-21157) which includes a 10-residue sequence from c-myc, the pFLAG system (International Biotechnologies, Inc.), the pEZZ-protein A system (Pharmacia, N.J.), and a 16 amino acid portion of the Haemophilus influenza hemagglutinin protein. Furthermore, any polypeptide can be used as a Tag so long as a reagent, e.g., an antibody interacting specifically with the Tag polypeptide is available or can be prepared or identified.

As indicated by the examples set out below, nucleic acids can be obtained from mRNA present in any of a number of eukaryotic cells, e.g., and are preferably obtained from metazoan cells, more preferably from vertebrate cells, and even more preferably from mammalian cells. It should also be possible to obtain nucleic acids of the present invention from genomic DNA from both adults and embryos. For example, a gene can be cloned from either a cDNA or a genomic library in accordance with protocols generally known to persons skilled in the art. cDNA can be obtained by isolating total mRNA from a cell, e.g., a vertebrate cell, a mammalian cell, or a human cell, including embryonic cells. Double stranded cDNAs can then be prepared from the total mRNA, and subsequently inserted into a suitable plasmid or bacteriophage vector using any one of a number of known techniques. The gene can also be cloned using established polymerase chain reaction techniques in accordance with the nucleotide sequence information provided by the invention.

In certain embodiments, a nucleic acid, probe, vector, or other construct of the present invention includes at least about five, at least about ten, or at least about twenty nucleic acids from a region designated as novel in Table 2. In certain other embodiments, a nucleic acid of the present invention includes at least about five, at least about ten, or at least about twenty nucleic acids which are not included in the clones whose accession numbers are listed in Table 2.

The invention includes within its scope a polynucleotide having the nucleotide sequence of nucleic acid obtained from this biological material, wherein the nucleic acid hybridizes under stringent conditions (at least about 4×SSC at 65° C., or at least about 4×SSC at 42° C.; see, for example, U.S. Pat. No. 5,707,829, incorporated herein by reference) with at least 15 contiguous nucleotides of at least one of SEQ ID Nos. 1-544. By this is intended that when at least 15 contiguous nucleotides of one of SEQ ID Nos. 1-544 is used as a probe, the probe will preferentially hybridize with a gene or mRNA (of the biological material) comprising the complementary sequence, allowing the identification and retrieval of the nucleic acids of the biological material that uniquely hybridize to the selected probe. Probes from more than one of SEQ ID Nos. 1-544 will hybridize with the same gene or mRNA if the cDNA from which they were derived corresponds to one mRNA. Probes of more than 15 nucleotides can be used, but 15 nucleotides represents enough sequence for unique identification.

Because the present nucleic acids represent partial mRNA transcripts, two or more nucleic acids of the invention may represent different regions of the same mRNA transcript and the same gene. Thus, if two or more of SEQ ID Nos. 1-544 are identified as belonging to the same clone, then either sequence can be used to obtain the full-length mRNA or gene.

Nucleic acid-related polynucleotides can also be isolated from cDNA libraries. These libraries are preferably prepared from mRNA of human colon cells, more preferably, human colon cancer specific tissue, designated as the DE clones in the appended Tables. In another embodiment the nucleic acids are isolated from libraries prepared from normal colon specific tissue, designated herein as PA clones in the appended Tables. In yet another embodiment, this invention discloses nucleic acid sequences that can be isolated from both libraries prepared from a human colon adenocarcinoma cell line, SW480, as well as from libraries prepared from either normal colon specific tissue or from colon cancer specific tissue. These sequences are listed in Table 3. Alignment of SEQ ID Nos. 1-544, as described above, can indicated that a cell line or tissue source of a related protein or polynucleotide can also be used as a source of the nucleic acid-related cDNA.

Techniques for producing and probing nucleic acid sequence libraries are described, for example, in Sambrook et al., “Molecular Cloning: A Laboratory Manual” (New York, Cold Spring Harbor Laboratory, 1989). The eDNA can be prepared by using primers based on a sequence from SEQ ID Nos. 1-544. In one embodiment, the cDNA library can be made from only poly-adenylated mRNA. Thus, poly-T primers can be used to prepare cDNA from the mRNA. Alignment of SEQ ID Nos. 1-544 can result in identification of a related polypeptide or polynucleotide. Some of the polynucleotides disclosed herein contains repetitive regions that were subject to masking during the search procedures. The information about the repetitive regions is discussed below.

Constructs of polynucleotides having sequences of SEQ ID Nos. 1-544 can be generated synthetically. Alternatively, single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides is described by Stemmer et al., Gene ( Amsterdam ) (1995) 164(1):49-53. In this method, assembly PCR (the synthesis of long DNA sequences from large numbers of oligodeoxyribonucleotides (oligos)) is described. The method is derived from DNA shuffling (Stemmer, Nature (1994) 370:389-391), and does not rely on DNA ligase, but instead relies on DNA polymerase to build increasingly longer DNA fragments during the assembly process. For example, a 1.1-kb fragment containing the TEM-1 beta-lactamase-encoding gene (bla) can be assembled in a single reaction from a total of 56 oligos, each 40 nucleotides (nt) in length. The synthetic gene can be PCR amplified and cloned in a vector containing the tetracycline-resistance gene (Tc-R) as the sole selectable marker. Without relying on ampicillin (Ap) selection, 76% of the Tc-R colonies were Ap-R, making this approach a general method for the rapid and cost-effective synthesis of any gene.

IV. Identification of Functional and Structural Motifs of Novel Genes Using Art-Recognized Methods

Translations of the nucleotide sequence of the nucleic acids, cDNAs, or full genes can be aligned with individual known sequences. Similarity with individual sequences can be used to determine the activity of the polypeptides encoded by the polynucleotides of the invention. For example, sequences that show similarity with a chemokine sequence may exhibit chemokine activities. Also, sequences exhibiting similarity with more than one individual sequence may exhibit activities that are characteristic of either or both individual sequences.

The full length sequences and fragments of the polynucleotide sequences of the nearest neighbors can be used as probes and primers to identify and isolate the full length sequence of the nucleic acid. The nearest neighbors can indicate a tissue or cell type to be used to construct a library for the full-length sequences of the nucleic acid.

Typically, the nucleic acids are translated in all six frames to determine the best alignment with the individual sequences. The sequences disclosed herein in the Sequence Listing are in a 5′ to 3′ orientation and translation in three frames can be sufficient (with a few specific exceptions as described in the Examples). These amino acid sequences are referred to, generally, as query sequences, which will be aligned with the individual sequences.

Nucleic acid sequences can be compared with known genes by any of the methods disclosed above. Results of individual and query sequence alignments can be divided into three categories: high similarity, weak similarity, and no similarity. Individual alignment results ranging from high similarity to weak similarity provide a basis for determining polypeptide activity and/or structure.

Parameters for categorizing individual results include: percentage of the alignment region length where the strongest alignment is found, percent sequence identity, and p value.

The percentage of the alignment region length is calculated by counting the number of residues of the individual sequence found in the region of strongest alignment. This number is divided by the total residue length of the query sequence to find a percentage. An example is shown below:

The region of alignment begins at amino acid 9 and ends at amino acid 19. The total length of the query sequence is 20 amino acids. The percent of the alignment region length is 11/20 or 55%.

Percent sequence identity is calculated by counting the number of amino acid matches between the query and individual sequence and dividing total number of matches by the number of residues of the individual sequence found in the region of strongest alignment. For the example above, the percent identity would be 10 matches divided by 11 amino acids, or approximately 90.9%.

P value is the probability that the alignment was produced by chance. For a single alignment, the p value can be calculated according to Karlin et al., Proc. Natl. Acad. Sci. 87: 2264 (1990) and Karlin et al., Proc. Natl. Acad. Sci. 90: (1993). The p value of multiple alignments using the same query sequence can be calculated using an heuristic approach described in Altschul et al., Nat. Genet. 6: 119 (1994). Alignment programs such as BLAST program can calculate the p value.

The boundaries of the region where the sequences align can be determined according to Doolittle, Methods in Enzymology, supra; BLAST or FASTA programs; or by determining the area where the sequence identity is highest.

Another factor to consider for determining identity or similarity is the location of the similarity or identity. Strong local alignment can indicate similarity even if the length of alignment is short. Sequence identity scattered throughout the length of the query sequence also can indicate a similarity between the query and profile sequences.

High Similarity

For the alignment results to be considered high similarity, the percent of the alignment region length, typically, is at least about 55% of total length query sequence; more typically, at least about 58%; even more typically; at least about 60% of the total residue length of the query sequence. Usually, percent length of the alignment region can be as much as about 62%; more usually, as much as about 64%; even more usually, as much as about 66%.

Further, for high similarity, the region of alignment, typically, exhibits at least about 75% of sequence identity; more typically, at least about 78%; even more typically; at least about 80% sequence identity. Usually, percent sequence identity can be as much as about 82%; more usually, as much as about 84%; even more usually, as much as about 86%.

The p value is used in conjunction with these methods. If high similarity is found, the query sequence is considered to have high similarity with a profile sequence when the p value is less than or equal to about 10 −2 ; more usually; less than or equal to about 10 −3 ; even more usually; less than or equal to about 10 −4 . More typically, the p value is no more than about 10 −5 ; more typically; no more than or equal to about 10 −10 ; even more typically; no more than or equal to about 10 −15 for the query sequence to be considered high similarity.

Weak Similarity

For the alignment results to be considered weak similarity, there is no minimum percent length of the alignment region nor minimum length of alignment. A better showing of weak similarity is considered when the region of alignment is, typically, at least about 15 amino acid residues in length; more typically, at least about 20; even more typically; at least about 25 amino acid residues in length. Usually, length of the alignment region can be as much as about 30 amino acid residues; more usually, as much as about 40; even more usually, as much as about 60 amino acid residues.

Further, for weak similarity, the region of alignment, typically, exhibits at least about 35% of sequence identity; more typically, at least about 40%; even more typically; at least about 45% sequence identity. Usually, percent sequence identity can be as much as about 50%; more usually, as much as about 55%; even more usually, as much as about 60%.

If low similarity is found, the query sequence is considered to have weak similarity with a profile sequence when the p value is usually less than or equal to about 10 −2 ; more usually; less than or equal to about 10 −3 ; even more usually; less than or equal to about 10 −4 . More typically, the p value is no more than about 10 −5 ; more usually; no more than or equal to about 10 −10 ; even more usually; no more than or equal to about 10 −15 for the query sequence to be considered weak similarity.

Similarity Determined by Sequence Identity

Sequence identity alone can be used to determine similarity of a query sequence to an individual sequence and can indicate the activity of the sequence. Such an alignment, preferably, permits gaps to align sequences. Typically, the query sequence is related to the profile sequence if the sequence identity over the entire query sequence is at least about 15%; more typically, at least about 20%; even more typically, at least about 25%; even more typically, at least about 50%. Sequence identity alone as a measure of similarity is most useful when the query sequence is usually, at least 80 residues in length; more usually, 90 residues; even more usually, at least 95 amino acid residues in length. More typically, similarity can be concluded based on sequence identity alone when the query sequence is preferably 100 residues in length; more preferably, 120 residues in length; even more preferably, 150 amino acid residues in length.

Determining Activity from Alignments with Profile and Multiple Aligned Sequences

Translations of the nucleic acids can be aligned with amino acid profiles that define either protein families or common motifs. Also, translations of the nucleic acids can be aligned to multiple sequence alignments (MSA) comprising the polypeptide sequences of members of protein families or motifs. Similarity or identity with profile sequences or MSAs can be used to determine the activity of the polypeptides encoded by nucleic acids or corresponding cDNA or genes. For example, sequences that show an identity or similarity with a chemokine profile or MSA can exhibit chemokine activities.

Profiles can designed manually by (1) creating a MSA, which is an alignment of the amino acid sequence of members that belong to the family and (2) constructing a statistical representation of the alignment. Such methods are described, for example, in Birney et al., Nucl. Acid Res. 24(14 : 2730-2739 (1996).

MSAs of some protein families and motifs are publicly available. For example, these include MSAs of 547 different families and motifs. These MSAs are described also in Sonnhammer et al., Proteins 28: 405-420 (1997). Other sources are also available in the world wide web. A brief description of these MSAs is reported in Pascarella et al., Prot. Eng. 9(3): 249-251 (1996).

Techniques for building profiles from MSAs are described in Sonnhammer et al., supra; Birney et al., supra; and Methods in Enzymology , vol. 266: “Computer Methods for Macromolecular Sequence Analysis,” 1996, ed. Doolittle, Academic Press, Inc., a division of Harcourt Brace & Co., San Diego, Calif., USA.

Similarity between a query sequence and a protein family or motif can be determined by (a) comparing the query sequence against the profile and/or (b) aligning the query sequence with the members of the family or motif.

Typically, a program such as Searchwise can be used to compare the query sequence to the statistical representation of the multiple alignment, also known as a profile. The program is described in Birney et al., supra. Other techniques to compare the sequence and profile are described in Sonnhammer et al., supra and Doolittle, supra.

Next, methods described by Feng et al., J. Mol. Evol. 25: 351-360 (1987) and Higgins et al., CABIOS 5: 151-153 (1989) can be used align the query sequence with the members of a family or motif, also known as a MSA. Computer programs, such as PILEUP, can be used. See Feng et al., infra.

The following factors are used to determine if a similarity between a query sequence and a profile or MSA exists: (1) number of conserved residues found in the query sequence, (2) percentage of conserved residues found in the query sequence, (3) number of frameshifts, and (4) spacing between conserved residues.

Some alignment programs that both translate and align sequences can make any number of frameshifts when translating the nucleotide sequence to produce the best alignment. The fewer frameshifts needed to produce an alignment, the stronger the similarity or identity between the query and profile or MSAs. For example, a weak similarity resulting from no frameshifts can be a better indication of activity or structure of a query sequence, than a strong similarity resulting from two frameshifts. Preferably, three or fewer frameshifts are found in an alignment; more preferably two or fewer frameshifts; even more preferably, one or fewer frameshifts; even more preferably, no frameshifts are found in an alignment of query and profile or MSAs.

Conserved residues are those amino acids that are found at a particular position in all or some of the family or motif members. For example, most known chemokines contain four conserved cysteines. Alternatively, a position is considered conserved if only a certain class of amino acids is found in a particular position in all or some of the family members. For example, the N-terminal position may contain a positively charged amino acid, such as lysine, arginine, or histidine.

Typically, a residue of a polypeptide is conserved when a class of amino acids or a single amino acid is found at a particular position in at least about 40% of all class members; more typically, at least about 50%; even more typically, at least about 60% of the members. Usually, a residue is conserved when a class or single amino acid is found in at least about 70% of the members of a family or motif; more usually, at least about 80%; even more usually, at least about 90%; even more usually, at least about 95%.

A residue is considered conserved when three unrelated amino acids are found at a particular position in the some or all of the members; more usually, two unrelated amino acids. These residues are conserved when the unrelated amino acids are found at particular positions in at least about 40% of all class member; more typically, at least about 50%; even more typically, at least about 60% of the members. Usually, a residue is conserved when a class or single amino acid is found in at least about 70% of the members of a family or motif; more usually, at least about 80%; even more usually, at least about 90%; even more usually, at least about 95%.

A query sequence has similarity to a profile or MSA when the query sequence comprises at least about 25% of the conserved residues of the profile or MSA; more usually, at least about 30%; even more usually; at least about 40%. Typically, the query sequence has a stronger similarity to a profile sequence or MSA when the query sequence comprises at least about 45% of the conserved residues of the profile or MSA; more typically, at least about 50%; even more typically; at least about 55%.

V. Probes and Primers

The nucleotide sequences determined from the cloning of genes from tumor cells, especially colon cancer cell lines and tissues will further allow for the generation of probes and primers designed for identifying and/or cloning homologs in other cell types, e.g., from other tissues, as well as homologs from other mammalian organisms. Nucleotide sequences useful as probes/primers may include all or a portion of the sequences listed in SEQ ID Nos. 1-544 or sequences complementary thereto or sequences which hybridize under stringent conditions to all or a portion of SEQ ID Nos. 1-544. For instance, the present invention also provides a probe/primer comprising a substantially purified oligonucleotide, which oligonucleotide comprising a nucleotide sequence that hybridizes under stringent conditions to at least approximately 12, preferably 25, more preferably 40, 50, or 75 consecutive nucleotides up to the full length of the sense or anti-sense sequence selected from the group consisting of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, or naturally occurring mutants thereof. For instance, primers based on a nucleic acid represented in SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, can be used in PCR reactions to clone homologs of that sequence.

In yet another embodiment, the invention provides probes/primers comprising a nucleotide sequence that hybridizes under moderately stringent conditions to at least approximately 12, 16, 25, 40, 50 or 75 consecutive nucleotides up to the full length of the sense or antisense sequence selected from the group consisting of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or naturally occurring mutants thereof.

In particular, these probes are useful because they provide a method for detecting mutations in wild-type genes of the present invention. Nucleic acid probes which are complementary to a wild-type gene of the present invention and can form mismatches with mutant genes are provided, allowing for detection by enzymatic or chemical cleavage or by shifts in electrophoretic mobility.

Likewise, probes based on the subject sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins, for use, for example, in prognostic or diagnostic assays. In preferred embodiments, the probe further comprises a label group attached thereto and able to be detected, e.g., the label group is selected from radioisotopes, fluorescent compounds, chemiluminescent compounds, enzymes, and enzyme co-factors.

Full-length cDNA molecules comprising the disclosed nucleic acids are obtained as follows. A subject nucleic acid or a portion thereof comprising at least about 12, 15, 18, or 20 nucleotides up to the full length of a sequence represented in SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, may be used as a hybridization probe to detect hybridizing members of a cDNA library using probe design methods, cloning methods, and clone selection techniques as described in U.S. Pat. No. 5,654,173, “Secreted Proteins and Polynucleotides Encoding Them,” incorporated herein by reference. Libraries of cDNA may be made from selected tissues, such as normal or tumor tissue, or from tissues of a mammal treated with, for example, a pharmaceutical agent. Preferably, the tissue is the same as that used to generate the nucleic acids, as both the nucleic acid and the cDNA represent expressed genes. Most preferably, the cDNA library is made from the biological material described herein in the Examples. Alternatively, many cDNA libraries are available commercially. (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2 nd Ed. (Cold Spring Harbor Press, Cold Spring Harbor, N.Y. 1989). The choice of cell type for library construction may be made after the identity of the protein encoded by the nucleic acid-related gene is known. This will indicate which tissue and cell types are likely to express the related gene, thereby containing the mRNA for generating the cDNA.

Members of the library that are larger than the nucleic acid, and preferably that contain the whole sequence of the native message, may be obtained. To confirm that the entire cDNA has been obtained, RNA protection experiments may be performed as follows. Hybridization of a full-length cDNA to an mRNA may protect the RNA from RNase degradation. If the cDNA is not full length, then the portions of the mRNA that are not hybridized may be subject to RNase degradation. This may be assayed, as is known in the art, by changes in electrophoretic mobility on polyacrylamide gels, or by detection of released monoribonucleotides. Sambrook et al., Molecular Cloning: A Laboratory Manual, 2 nd Ed. (Cold Spring Harbor Press, Cold Spring Harbor, N.Y. 1989). In order to obtain additional sequences 5′ to the end of a partial cDNA, 5′ RACE (PCR Protocols: A Guide to Methods and Applications (Academic Press, Inc. 1990)) may be performed.

Genomic DNA may be isolated using nucleic acids in a manner similar to the isolation of full-length cDNAs. Briefly, the nucleic acids, or portions thereof, may be used as probes to libraries of genomic DNA. Preferably, the library is obtained from the cell type that was used to generate the nucleic acids. Most preferably, the genomic DNA is obtained from the biological material described herein in the Example. Such libraries may be in vectors suitable for carrying large segments of a genome, such as P1 or YAC, as described in detail in Sambrook et al., 9.4-9.30. In addition, genomic sequences can be isolated from human BAC libraries, which are commercially available from Research Genetics, Inc., Huntville, Ala., USA, for example. In order to obtain additional 5′ or 3′ sequences, chromosome walking may be performed, as described in Sambrook et al., such that adjacent and overlapping fragments of genomic DNA are isolated. These may be mapped and pieced together, as is known in the art, using restriction digestion enzymes and DNA ligase.

Using the nucleic acids of the invention, corresponding full length genes can be isolated using both classical and PCR methods to construct and probe cDNA libraries. Using either method, Northern blots, preferably, may be performed on a number of cell types to determine which cell lines express the gene of interest at the highest rate.

Classical methods of constructing cDNA libraries are taught in Sambrook et al., supra. With these methods, cDNA can be produced from mRNA and inserted into viral or expression vectors. Typically, libraries of mRNA comprising poly(A) tails can be produced with poly(T) primers. Similarly, cDNA libraries can be produced using the instant sequences as primers.

PCR methods may be used to amplify the members of a cDNA library that comprise the desired insert. In this case, the desired insert may contain sequence from the full length cDNA that corresponds to the instant nucleic acids. Such PCR methods include gene trapping and RACE methods.

Gene trapping may entail inserting a member of a cDNA library into a vector. The vector then may be denatured to produce single stranded molecules. Next, a substrate-bound probe, such a biotinylated oligo, may be used to trap cDNA inserts of interest. Biotinylated probes can be linked to an avidin-bound solid substrate. PCR methods can be used to amplify the trapped cDNA. To trap sequences corresponding to the full length genes, the labeled probe sequence may be based on the nucleic acids of the invention, e.g., SEQ ID Nos. 1-1 68, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto. Random primers or primers specific to the library vector can be used to amplify the trapped cDNA. Such gene trapping techniques are described in Gruber et al., PCT WO 95/04745 and Gruber et al., U.S. Pat. No. 5,500,356. Kits are commercially available to perform gene trapping experiments from, for example, Life Technologies, Gaithersburg, Maryland, USA.

“Rapid amplification of cDNA ends,” or RACE, is a PCR method of amplifying cDNAs from a number of different RNAs. The cDNAs may be ligated to an oligonucleotide linker and amplified by PCR using two primers. One primer may be based on sequence from the instant nucleic acids, for which full length sequence is desired, and a second primer may comprise a sequence that hybridizes to the oligonucleotide linker to amplify the cDNA. A description of this method is reported in PCT Pub. No. WO 97/19110.

In preferred embodiments of RACE, a common primer may be designed to anneal to an arbitrary adaptor sequence ligated to cDNA ends (Apte and Siebert, Biotechniques 15:890-893, 1993; Edwards et al., Nuc. Acids Res. 19:5227-5232, 1991). When a single gene-specific RACE primer is paired with the common primer, preferential amplification of sequences between the single gene specific primer and the common primer occurs. Commercial cDNA pools modified for use in RACE are available.

Another PCR-based method generates full-length cDNA library with anchored ends without specific knowledge of the cDNA sequence. The method uses lock-docking primers (I-VI), where one primer, poly TV (I-III) locks over the polyA tail of eukaryotic mRNA producing first strand synthesis and a second primer, polyGH (IV-VI) locks onto the polyC tail added by terminal deoxynucleotidyl transferase (TdT). This method is described in PCT Pub. No. WO 96/40998.

The promoter region of a gene generally is located 5′ to the initiation site for RNA polymerase II. Hundreds of promoter regions contain the “TATA” box, a sequence such as TATTA or TATAA, which is sensitive to mutations. The promoter region can be obtained by performing 5′ RACE using a primer from the coding region of the gene. Alternatively, the cDNA can be used as a probe for the genomic sequence, and the region 5′ to the coding region is identified by “walking up.”

If the gene is highly expressed or differentially expressed, the promoter from the gene may be of use in a regulatory construct for a heterologous gene.

Once the full-length cDNA or gene is obtained, DNA encoding variants can be prepared by site-directed mutagenesis, described in detail in Sambrook et al., 15.3-15.63. The choice of codon or nucleotide to be replaced can be based on the disclosure herein on optional changes in amino acids to achieve altered protein structure and/or function.

As an alternative method to obtaining DNA or RNA from a biological material, nucleic acid comprising nucleotides having the sequence of one or more nucleic acids of the invention can be synthesized. Thus, the invention encompasses nucleic acid molecules ranging in length from 12 nucleotides (corresponding to at least 12 contiguous nucleotides which hybridize under stringent conditions to or are at least 80% identical to a nucleic acid represented by one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto) up to a maximum length suitable for one or more biological manipulations, including replication and expression, of the nucleic acid molecule. The invention includes but is not limited to (a) nucleic acid having the size of a full gene, and comprising at least one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto; (b) the nucleic acid of (a) also comprising at least one additional gene, operably linked to permit expression of a fusion protein; (c) an expression vector comprising (a) or (b); (d) a plasmid comprising (a) or (b); and (e) a recombinant viral particle comprising (a) or (b). Construction of (a) can be accomplished as described below in part IV.

The sequence of a nucleic acid of the present invention is not limited and can be any sequence of A, T, G, and/or C (for DNA) and A, U, G, and/or C (for RNA) or modified bases thereof, including inosine and pseudouridine. The choice of sequence will depend on the desired function and can be dictated by coding regions desired, the intron-like regions desired, and the regulatory regions desired.

VI. Vectors Carrying Nucleic Acids of the Present Invention

The invention further provides plasmids and vectors, which can be used to express a gene in a host cell. The host cell may be any prokaryotic or eukaryotic cell. Thus, a nucleotide sequence derived from any one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, encoding all or a selected portion of a protein, can be used to produce a recombinant form of an polypeptide via microbial or eukaryotic cellular processes. Ligating the polynucleotide sequence into a gene construct, such as an expression vector, and transforming or transfecting into hosts, either eukaryotic (yeast, avian, insect or mammalian) or prokaryotic (bacterial cells), are standard procedures well known in the art.

Vectors that allow expression of a nucleic acid in a cell are referred to as expression vectors. Typically, expression vectors contain a nucleic acid operably linked to at least one transcriptional regulatory sequence. Regulatory sequences are art-recognized and are selected to direct expression of the subject nucleic acids. Transcriptional regulatory sequences are described in Goeddel; Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990). In one embodiment, the expression vector includes a recombinant gene encoding a peptide having an agonistic activity of a subject polypeptide, or alternatively, encoding a peptide which is an antagonistic form of a subject polypeptide.

The choice of plasmid will depend on the type of cell in which propagation is desired and the purpose of propagation. Certain vectors are useful for amplifying and making large amounts of the desired DNA sequence. Other vectors are suitable for expression in cells in culture. Still other vectors are suitable for transfer and expression in cells in a whole animal or person. The choice of appropriate vector is well within the skill of the art. Many such vectors are available commercially. The nucleic acid or full-length gene is inserted into a vector typically by means of DNA ligase attachment to a cleaved restriction enzyme site in the vector. Alternatively, the desired nucleotide sequence may be inserted by homologous recombination in vivo. Typically this is accomplished by attaching regions of homology to the vector on the flanks of the desired nucleotide sequence. Regions of homology are added by ligation of oligonucleotides, or by polymerase chain reaction using primers comprising both the region of homology and a portion of the desired nucleotide sequence.

Nucleic acids or full-length genes are linked to regulatory sequences as appropriate to obtain the desired expression properties. These may include promoters (attached either at the 5′ end of the sense strand or at the 3′ end of the antisense strand), enhancers, terminators, operators, repressors, and inducers. The promoters may be regulated or constitutive. In some situations it may be desirable to use conditionally active promoters, such as tissue-specific or developmental stage-specific promoters. These are linked to the desired nucleotide sequence using the techniques described above for linkage to vectors. Any techniques known in the art may be used.

When any of the above host cells, or other appropriate host cells or organisms, are used to replicate and/or express the polynucleotides or nucleic acids of the invention, the resulting replicated nucleic acid, RNA, expressed protein or polypeptide, is within the scope of the invention as a product of the host cell or organism. The product is recovered by any appropriate means known in the art.

Once the gene corresponding to the nucleic acid is identified, its expression can be regulated in the cell to which the gene is native. For example, an endogenous gene of a cell can be regulated by an exogenous regulatory sequence as disclosed in U.S. Pat. No. 5,641,670, “Protein Production and Protein Delivery.” A number of vectors exist for the expression of recombinant proteins in yeast (see, for example, Broach et al. (1983) in Experimental Manipulation of Gene Expression, ed. M. Inouye, Academic Press, p. 83, incorporated by reference herein). In addition, drug resistance markers such as ampicillin can be used. In an illustrative embodiment, a polypeptide is produced recombinantly utilizing an expression vector generated by sub-cloning one of the nucleic acids represented in one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto.

The preferred mammalian expression vectors contain both prokaryotic sequences, to facilitate the propagation of the vector in bacteria, and one or more eukaryotic transcription units that are expressed in eukaryotic cells. The various methods employed in the preparation of plasmids and transformation of host organisms are well known in the art. For other suitable expression systems for both prokaryotic and eukaryotic cells, as well as general recombinant procedures, see Molecular Cloning: A Laboratory Manual, 2 nd Ed., ed. by Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory Press: 1989) Chapters 16 and 17. When it is desirable to express only a portion of a gene, e.g., a truncation mutant, it may be necessary to add a start codon (ATG) to the oligonucleotide fragment containing the desired sequence to be expressed. It is well known in the art that a methionine at the N-terminal position can be enzyrnatically cleaved by the use of the enzyme methionine aminopeptidase (MAP). MAP has been cloned from E. coli (Ben-Bassat et al. (1987) J. Bacteriol. 169:751-757) and Salmonella typhimurium and its in vitro activity has been demonstrated on recombinant proteins (Miller et al. (1987) PNAS 84:2718-1722). Therefore, removal of an N-terminal methionine, if desired, can be achieved either in vivo by expressing polypeptides in a host which produces MAP (e.g., E. coli or CM89 or S. cerevisiae), or in vitro by use of purified MAP (e.g., procedure of Miller et al., supra).

Moreover, the nucleic acid constructs of the present invention can also be used as part of a gene therapy protocol to deliver nucleic acids such as antisense nucleic acids. Thus, another aspect of the invention features expression vectors for in vivo or in vitro transfection with an antisense oligonucleotide.

In addition to viral transfer methods, non-viral methods can also be employed to introduce a subject nucleic acid, e.g., a sequence represented by one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, into the tissue of an animal. Most nonviral methods of gene transfer rely on normal mechanisms used by mammalian cells for the uptake and intracellular transport of macromolecules. In preferred embodiments, non-viral targeting means of the present invention rely on endocytic pathways for the uptake of the subject nucleic acid by the targeted cell. Exemplary targeting means of this type include liposomal derived systems, polylysine conjugates, and artificial viral envelopes.

A nucleic acid of any of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, the corresponding cDNA, or the full-length gene may be used to express the partial or complete gene product. Appropriate nucleic acid constructs are purified using standard recombinant DNA techniques as described in, for example, Sambrook et al., (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor Press, Cold Spring Harbor, New York), and under current regulations described in United States Dept. of HHS, National Institute of Health (NIH) Guidelines for Recombinant DNA Research. The polypeptides encoded by the nucleic acid may be expressed in any expression system, including, for example, bacterial, yeast, insect, amphibian and mammalian systems. Suitable vectors and host cells are described in U.S. Pat. No. 5,654,173.

Bacteria. Expression systems in bacteria include those described in Chang et al., Nature (1978) 275:615, Goeddel et al., Nature (1979) 281:544, Goeddel et al., Nucleic Acids Res. (1980) 8:4057; EP 0 036,776, U.S. Pat. No. 4,551,433, DeBoer et al., Proc. Natl. Acad. Sci. ( USA ) (1983) 80:2125, and Siebenlist et al., Cell (1980) 20:269.

Yeast. Expression systems in yeast include those described in Hinnen et al., Proc. Natl. Acad. Sci. ( USA ) (1978) 75:1929; Ito et al., J. Bacteriol. (1983) 153:163; Kurtz et al., Mol. Cell. Biol. (1986) 6:142; Kunze et al., J. Basic Microbiol. (1985) 25:141; Gleeson et al., J. Gen. Microbiol. (1986) 132:3459, Roggenkamp et al., Mol. Gen. Genet. (1986) 202:302) Das et al., J. Bacteriol. (1984) 158:1165; De Louvencourt et al., J. Bacteriol. (1983) 154:737, Van den Berg et al., Bio/Technology (1990)8:135; Kunze et al., J. Basic Microbiol. (1985)25:141; Cregg et al., Mol. Cell. Biol. (1985) 5:3376, U.S. Pat. Nos. 4,837,148 and 4,929,555; Beach and Nurse, Nature (1981) 300:706; Davidow et al., Curr. Genet. (1985) 10:380, Gaillardin et al., Curr. Genet. (1985) 10:49, Ballance et al., Biochem. Biophys. Res. Commun. (1983) 112:284289; Tilburn et al., Gene (1983) 26:205221, Yelton et al., Proc. Natl. Acad. Sci. ( USA ) (1984) 81:14701474, Kelly and Hynes, EMBO J. (1985) 4:475479; EP 0 244,234, and WO 91/00357.

Insect Cells. Expression of heterologous genes in insects is accomplished as described in U.S. Pat. No. 4,745,051, Friesen et al. (1986) “The Regulation of Baculovirus Gene Expression” in: The Molecular Biology Of Baculoviruses (W. Doerfler, ed.), EP 0 127,839, EP 0 155,476, and Vlak et al., J. Gen. Virol. (1988) 69:765776, Miller et al., Ann. Rev. Microbiol. (1988) 42:177, Carbonell et al., Gene (1988) 73:409, Maeda et al., Nature (1985) 315:592594, Lebacq Verheyden et al., Mol. Cell. Biol. (1988) 8:3129; Smith et al., Proc. Natl. Acad. Sci. ( USA ) (1985) 82:8404, Miyajima et al., Gene (1987) 58:273; and Martin et al., DNA (1988) 7:99. Numerous baculoviral strains and variants and corresponding permissive insect host cells from hosts are described in Luckow et al., Bio/Technology (1988) 6:4755, Miller et al., Generic Engineering (Setlow, J. K. et al. eds.), Vol. 8 (Plenum Publishing, 1986), pp. 277279, and Maeda et al., Nature, (1985) 315:592-594.

Mammalian Cells. Mammalian expression is accomplished as described in Dijkema et al., EMBO J. (1985) 4:761, Gorman et al., Proc. Natl. Acad. Sci. ( USA ) (1982) 79:6777, Boshart et al., Cell (1985) 41:521 and U.S. Pat. No. 4,399,216. Other features of mammalian expression are facilitated as described in Ham and Wallace, Meth. Enz. (1979) 58:44, Barnes and Sato, Anal. Biochem. (1980) 102:255, U.S. Pat. Nos. 4,767,704, 4,657,866, 4,927,762, 4,560,655, WO 90/103430, WO 87/00195, and U.S. RE 30,985.

VII. Therapeutic Nucleic Acid Constructs

One aspect of the invention relates to the use of the isolated nucleic acid, e.g., SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, in antisense therapy. As used herein, antisense therapy refers to administration or in situ generation of oligonucleotide molecules or their derivatives which specifically hybridize (e.g., bind) under cellular conditions with the cellular mRNA and/or genomic DNA, thereby inhibiting transcription and/or translation of that gene. The binding may be by conventional base pair complementarity, or, for example, in the case of binding to DNA duplexes, through specific interactions in the major groove of the double helix. In general, antisense therapy refers to the range of techniques generally employed in the art, and includes any therapy which relies on specific binding to oligonucleotide sequences.

An antisense construct of the present invention can be delivered, for example, as an expression plasmid which, when transcribed in the cell, produces RNA which is complementary to at least a unique portion of the cellular mRNA. Alternatively, the antisense construct is an oligonucleotide probe which is generated ex vivo and which, when introduced into the cell, causes inhibition of expression by hybridizing with the mRNA and/or genomic sequences of a subject nucleic acid. Such oligonucleotide probes are preferably modified oligonucleotides which are resistant to endogenous nucleases, e.g., exonucleases and/or endonucleases, and are therefore stable in vivo. Exemplary nucleic acid molecules for use as antisense oligonucleotides are phosphoramidate, phosphorothioate and methylphosphonate analogs of DNA (see also U.S. Pat. Nos. 5,176,996; 5,264,564; and 5,256,775). Additionally, general approaches to constructing oligomers useful in antisense therapy have been reviewed, for example, by Van der Krol et al. (1988) BioTechniques 6:958-976; and Stein et al. (1988) Cancer Res 48:2659-2668. With respect to antisense DNA, oligodeoxyribonucleotides derived from the translation initiation site, e.g., between the −10 and +10 regions of the nucleotide sequence of interest, are preferred.

Antisense approaches involve the design of oligonucleotides (either DNA or RNA) that are complementary to mRNA. The antisense oligonucleotides will bind to the mRNA transcripts and prevent translation. Absolute complementarity, although preferred, is not required. In the case of double-stranded antisense nucleic acids, a single strand of the duplex DNA may thus be tested, or triplex formation may be assayed. The ability to hybridize will depend on both the degree of complementarity and the length of the antisense nucleic acid. Generally, the longer the hybridizing nucleic acid, the more base mismatches with an RNA it may contain and still form a stable duplex (or triplex, as the case may be). One skilled in the art can ascertain a tolerable degree of mismatch by use of standard procedures to determine the melting point of the hybridized complex.

Oligonucleotides that are complementary to the 5′ end of the mRNA, e.g., the 5′ untranslated sequence up to and including the AUG initiation codon, should work most efficiently at inhibiting translation. However, sequences complementary to the 3′ untranslated sequences of mRNAs have recently been shown to be effective at inhibiting translation of mRNAs as well. (Wagner, R. 1994. Nature 372:333). Therefore, oligonucleotides complementary to either the 5′ or 3′ untranslated, non-coding regions of a gene could be used in an antisense approach to inhibit translation of endogenous mRNA. Oligonucleotides complementary to the 5′ untranslated region of the mRNA should include the complement of the AUG start codon. Antisense oligonucleotides complementary to mRNA coding regions are typically less efficient inhibitors of translation but could also be used in accordance with the invention. Whether designed to hybridize to the 5′, 3′, or coding region of subject mRNA, antisense nucleic acids should be at least six nucleotides in length, and are preferably less that about 100 and more preferably less than about 50, 25, 17 or 10 nucleotides in length.

Regardless of the choice of target sequence, it is preferred that in vitro studies are first performed to quantitate the ability of the antisense oligonucleotide to quantitate the ability of the antisense oligonucleotide to inhibit gene expression. It is preferred that these studies utilize controls that distinguish between antisense gene inhibition and nonspecific biological effects of oligonucleotides. It is also preferred that these studies compare levels of the target RNA or protein with that of an internal control RNA or protein. Additionally, it is envisioned that results obtained using the antisense oligonucleotide are compared with those obtained using a control oligonucleotide. It is preferred that the control oligonucleotide is of approximately the same length as the test oligonucleotide and that the nucleotide sequence of the oligonucleotide differs from the antisense sequence no more than is necessary to prevent specific hybridization to the target sequence.

The oligonucleotides can be DNA or RNA or chimeric mixtures or derivatives or modified versions thereof, single-stranded or double-stranded. The oligonucleotide can be modified at the base moiety, sugar moiety, or phosphate backbone, for example, to improve stability of the molecule, hybridization, etc. The oligonucleotide may include other appended groups such as peptides (e.g., for targeting host cell receptors), or agents facilitating transport across the cell membrane (see, e.g., Letsinger et al., 1989, Proc. Natl. Acad. Sci. U.S.A. 86:6553-6556; Lemaitre et al., 1987, Proc. Natl. Acad. Sci. 84:648-652; PCT Publication No. WO 88/09810, published Dec. 15, 1988) or the blood-brain barrier (see, e.g., PCT Publication No. WO 89/10134, published Apr. 25, 1988), hybridization-triggered cleavage agents (See, e.g., Krol et al., 1988, BioTechniques 6:958-976), or intercalating agents (See, e.g., Zon, 1988, Pharm. Res. 5:539-549). To this end, the oligonucleotide may be conjugated to another molecule, e.g., a peptide, hybridization triggered cross-linking agent, transport agent, hybridization-triggered cleavage agent, etc.

The antisense oligonucleotide may comprise at least one modified base moiety which is selected from the group including but not limited to 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xantine, 4-acetylcytosine, 5-(carboxyhydroxytriethyl)uracil, 5-carboxymethylaminomethyl-2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methyl-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine.

The antisense oligonucleotide may also comprise at least one modified sugar moiety selected from the group including but not limited to arabinose, 2-fluoroarabinose, xylulose, and hexose.

The antisense oligonucleotide can also contain a neutral peptide-like backbone. Such molecules are termed peptide nucleic acid (PNA)-oligomers and are described, e.g., in Perry-O'Keefe et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93:14670 and in Eglom et al. (1993) Nature 365:566. One advantage of PNA oligomers is their capability to bind to complementary DNA essentially independently from the ionic strength of the medium due to the neutral backbone of the DNA. In yet another embodiment, the antisense oligonucleotide comprises at least one modified phosphate backbone selected from the group consisting of a phosphorothioate, a phosphorodithioate, a phosphoramidothioate, a phosphoramidate, a phosphordiamidate, a methylphosphonate, an alkyl phosphotriester, and a formacetal or analog thereof.

In yet a further embodiment, the antisense oligonucleotide is an α-anomeric oligonucleotide. An α-anomeric oligonucleotide forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual β-units, the strands run parallel to each other (Gautier et al., 1987, Nucl. Acids Res. 15:6625-6641). The oligonucleotide is a 2′-O-methylribonucleotide (Inoue et al., 1987, Nucl. Acids Res. 15:6131-12148), or a chimeric RNA-DNA analogue (Inoue et al., 1987, FEBS Lett. 215:327-330).

Oligonucleotides of the invention may be synthesized by standard methods known in the art, e.g., by use of an automated DNA synthesizer (such as are commercially available from Biosearch, Applied Biosystems, etc.). As examples, phosphorothioate oligonucleotides may be synthesized by the method of Stein et al. (1988, Nucl. Acids Res. 16:3209), methylphosphonate olgonucleotides can be prepared by use of controlled pore glass polymer supports (Sarin et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85:7448-7451), etc.

While antisense nucleotides complementary to a coding region sequence can be used, those complementary to the transcribed untranslated region and to the region comprising the initiating methionine are most preferred.

The antisense molecules can be delivered to cells which express the target nucleic acid in vivo. A number of methods have been developed for delivering antisense DNA or RNA to cells; e.g., antisense molecules can be injected directly into the tissue site, or modified antisense molecules, designed to target the desired cells (e.g., antisense linked to peptides or antibodies that specifically bind receptors or antigens expressed on the target cell surface) can be administered systemically.

However, it is often difficult to achieve intracellular concentrations of the antisense sufficient to suppress translation on endogenous mRNAs. Therefore, a preferred approach utilizes a recombinant DNA construct in which the antisense oligonucleotide is placed under the control of a strong pol III or pol II promoter. The use of such a construct to transfect target cells in the patient will result in the transcription of sufficient amounts of single stranded RNAs that will form complementary base pairs with the endogenous transcripts and thereby prevent translation of the target mRNA. For example, a vector can be introduced in vivo such that it is taken up by a cell and directs the transcription of an antisense RNA. Such a vector can remain episomal or become chromosomally integrated, as long as it can be transcribed to produce the desired antisense RNA. Such vectors can be constructed by recombinant DNA technology methods standard in the art. Vectors can be plasmid, viral, or others known in the art for replication and expression in mammalian cells. Expression of the sequence encoding the antisense RNA can be by any promoter known in the art to act in mammalian, preferably human cells. Such promoters can be inducible or constitutive. Such promoters include but are not limited to: the SV40 early promoter region (Bernoist and Chambon, 1981, Nature 290:304-310), the promoter contained in the 3′ long terminal repeat of Rous sarcoma virus (Yamamoto et al., 1980, Cell 22:787-797), the herpes thymidine kinase promoter (Wagner et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:1441-1445), the regulatory sequences of the metallothionein gene (Brinster et al, 1982, Nature 296:39-42), etc. Any type of plasmid, cosmid, YAC or viral vector can be used to prepare the recombinant DNA construct which can be introduced directly into the tissue site; e.g., the choroid plexus or hypothalamus. Alternatively, viral vectors can be used which selectively infect the desired tissue (e.g., for brain, herpesvirus vectors may be used), in which case administration may be accomplished by another route (e.g., systemically).

In another aspect of the invention, ribozyme molecules designed to catalytically cleave target mRNA transcripts can be used to prevent translation of target mRNA and expression of a target protein (See, e.g., PCT International Publication WO90/11364, published Oct. 4, 1990; Sarver et al., 1990, Science 247:1222-1225 and U.S. Pat. No. 5,093,246). While ribozymes that cleave mRNA at site specific recognition sequences can be used to destroy target mRNAs, the use of hammerhead ribozymes is preferred. Hammerhead ribozymes cleave mRNAs at locations dictated by flanking regions that form complementary base pairs with the target mRNA. The sole requirement is that the target mRNA have the following sequence of two bases: 5′-UG-3′. The construction and production of hammerhead ribozymes is well known in the art and is described more fully in Haseloff and Gerlach, 1988, Nature, 334:585-591. Preferably the ribozyme is engineered so that the cleavage recognition site is located near the 5′ end of the target mRNA; i.e., to increase efficiency and minimize the intracellular accumulation of non-functional mRNA transcripts.

The ribozymes of the present invention also include RNA endoribonucleases (hereinafter “Cech-type ribozymes”) such as the one which occurs naturally in Tetrahymena thermophila (known as the IVS, or L-19 IVS RNA) and which has been extensively described by Thomas Cech and collaborators (Zaug, et al., 1984, Science, 224:574-578; Zaug and Cech, 1986, Science, 231:470-475; Zaug, et al., 1986, Nature, 324:429-433; published International patent application No. W088/04300 by University Patents Inc.; Been and Cech, 1986, Cell, 47:207-216). The Cech-type ribozymes have an eight base pair active site which hybridizes to a target RNA sequence whereafter cleavage of the target RNA takes place. The invention encompasses those Cech-type ribozymes which target eight base-pair active site sequences that are present in a target gene.

As in the antisense approach, the ribozymes can be composed of modified oligonucleotides (e.g., for improved stability, targeting, etc.) and should be delivered to cells which express the target gene in vivo. A preferred method of delivery involves using a DNA construct “encoding” the ribozyme under the control of a strong constitutive pol III or pol II promoter, so that transfected cells will produce sufficient quantities of the ribozyme to destroy endogenous messages and inhibit translation. Because ribozymes, unlike antisense molecules, are catalytic, a lower intracellular concentration is required for efficiency.

Antisense RNA, DNA, and ribozyme molecules of the invention may be prepared by any method known in the art for the synthesis of DNA and RNA molecules. These include techniques for chemically synthesizing oligodeoxyribonucleotides and oligoribonucleotides well known in the art such as for example solid phase phosphoramidite chemical synthesis. Alternatively, RNA molecules may be generated by in vitro and in vivo transcription of DNA sequences encoding the antisense RNA molecule. Such DNA sequences may be incorporated into a wide variety of vectors which incorporate suitable RNA polymerase promoters such as the T7 or SP6 polymerase promoters. Alternatively, antisense cDNA constructs that synthesize antisense RNA constitutively or inducibly, depending on the promoter used, can be introduced stably into cell lines.

Moreover, various well-known modifications to nucleic acid molecules may be introduced as a means of increasing intracellular stability and half-life. Possible modifications include but are not limited to the addition of flanking sequences of ribonucleotides or deoxyribonucleotides to the 5′ and/or 3′ ends of the molecule or the use of phosphorothioate or 2′ O-methyl rather than phosphodiesterase linkages within the oligodeoxyribonucleotide backbone.

VIII. Polypeptides of the Present Invention

The present invention makes available isolated polypeptides which are isolated from, or otherwise substantially free of other cellular proteins, especially other signal transduction factors and/or transcription factors which may normally be associated with the polypeptide. Subject polypeptides of the present invention include polypeptides encoded by the nucleic acids of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, or polypeptides encoded by genes of which a sequence in SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, is a fragment. Polypeptides of the present invention include those proteins which are differentially regulated in tumor cells, especially colon cancer-derived cell lines (relative to normal cells, e.g., normal colon tissue and non-colon tissue). In preferred embodiments, the polypeptides are upregulated in tumor cells, especially colon cancer cancer-derived cell lines. In other embodiments, the polypeptides are downregulated in tumor cells, especially colon cancer-derived cell lines. Proteins which are upregulated, such as oncogenes, or downregulated, such as tumor suppressors, in aberrantly proliferating cells may be targets for diagnostic or therapeutic techniques. For example, upregulation of the cdc2 gene induces mitosis. Overexpression of the myt1 gene, a mitotic deactivator, negatively regulates the activity of cdc2. Aberrant proliferation may thus be induced either by upregulating cdc2 or by downregulating myt1.

The term “substantially free of other cellular proteins” (also referred to herein as “contaminating proteins”) or “substantially pure or purified preparations” are defined as encompassing preparations of polypeptides having less than about 20% (by dry weight) contaminating protein, and preferably having less than about 5% contaminating protein. Functional forms of the subject polypeptides can be prepared, for the first time, as purified preparations by using a cloned nucleic acid as described herein. Full length proteins or fragments corresponding to one or more particular motifs and/or domains or to arbitrary sizes, for example, at least about 5, 10, 25, 50, 75, or 100 amino acids in length are within the scope of the present invention.

For example, isolated polypeptides can be encoded by all or a portion of a nucleic acid sequence shown in any of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto. Isolated peptidyl portions of proteins can be obtained by screening peptides recombinantly produced from the corresponding fragment of the nucleic acid encoding such peptides. In addition, fragments can be chemically synthesized using techniques known in the art such as conventional Merrifield solid phase f-Moc or t-Boc chemistry. For example, a polypeptide of the present invention may be arbitrarily divided into fragments of desired length with no overlap of the fragments, or preferably divided into overlapping fragments of a desired length. The fragments can be produced (recombinantly or by chemical synthesis) and tested to identify those peptidyl fragments which can function as either agonists or antagonists of a wild-type (e.g., “authentic”) protein.

Another aspect of the present invention concerns recombinant forms of the subject proteins. Recombinant polypeptides preferred by the present invention, in addition to native proteins, as described above are encoded by a nucleic acid, which is at least 60%, more preferably at least 80%, and more preferably 85%, and more preferably 90%, and more preferably 95% identical to an amino acid sequence encoded by SEQ ID Nos. 1-544. Polypeptides which are encoded by a nucleic acid that is at least about 98-99% identical with the sequence of SEQ ID Nos. 1-544 are also within the scope of the invention. Also included in the present invention are peptide fragments comprising at least a portion of such a protein.

In a preferred embodiment, a polypeptide of the present invention is a mammalian polypeptide and even more preferably a human polypeptide. In particularly preferred embodiment, the polypeptide retains wild-type bioactivity. It will be understood that certain post-translational modifications, e.g., phosphorylation and the like, can increase the apparent molecular weight of the polypeptide relative to the unmodified polypeptide chain.

The present invention further pertains to recombinant forms of one of the subject polypeptides. Such recombinant polypeptides preferably are capable of functioning in one of either role of an agonist or antagonist of at least one biological activity of a wild-type (“authentic”) polypeptide of the appended sequence listing. The term “evolutionarily related to”, with respect to amino acid sequences of proteins, refers to both polypeptides having amino acid sequences which have arisen naturally, and also to mutational variants of human polypeptides which are derived, for example, by combinatorial mutagenesis.

In general, polypeptides referred to herein as having an activity (e.g., are “bioactive”) of a protein are defined as polypeptides which include an amino acid sequence encoded by all or a portion of the nucleic acid sequences shown in one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, and which mimic or antagonize all or a portion of the biological/biochemical activities of a naturally occurring protein. According to the present invention, a polypeptide has biological activity if it is a specific agonist or antagonist of a naturally occurring form of a protein.

Assays for determining whether a compound, e.g, a protein or variant thereof, has one or more of the above biological activities are well known in the art. In certain embodiments, the polypeptides of the present invention have activities such as those outlined above.

In another embodiment, the coding sequences for the polypeptide can be incorporated as a part of a fusion gene including a nucleotide sequence encoding a different polypeptide. This type of expression system can be useful under conditions where it is desirable to produce an immunogenic fragment of a polypeptide (see, for example, EP Publication No: 0259149; and Evans et al. (1989) Nature 339:385; Huang et al. (1988) J. Virol. 62:3855; and Schlienger et al. (1992) J. Virol. 66:2). In addition to utilizing fusion proteins to enhance immunogenicity, it is widely appreciated that fusion proteins can also facilitate the expression of proteins, and, accordingly, can be used in the expression of the polypeptides of the present invention (see, for example, Current Protocols in Molecular Biology, eds. Ausubel et al. (N.Y.: John Wiley & Sons, 1991)). In another embodiment, a fusion gene coding for a purification leader sequence, such as a poly-(His)/enterokinase cleavage site sequence at the N-terminus of the desired portion of the recombinant protein, can allow purification of the expressed fusion protein by affinity chromatography using a Ni 2+ metal resin. The purification leader sequence can then be subsequently removed by treatment with enterokinase to provide the purified protein (e.g., see Hochuli et al. (1987) J. Chromatography 411:177; and Janknecht et al. PNAS 88:8972).

Techniques for making fusion genes are known to those skilled in the art. Essentially, the joining of various DNA fragments coding for different polypeptide sequences is performed in accordance with conventional techniques, employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR amplification of nucleic acid fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive nucleic acid fragments which can subsequently be annealed to generate a chimeric nucleic acid sequence (see, for example, Current Protocols in Molecular Biology, eds. Ausubel et al. John Wiley & Sons: 1992).

The present invention further pertains to methods of producing the subject polypeptides. For example, a host cell transfected with a nucleic acid vector directing expression of a nucleotide sequence encoding the subject polypeptides can be cultured under appropriate conditions to allow expression of the peptide to occur. Suitable media for cell culture are well known in the art. The recombinant polypeptide can be isolated from cell culture medium, host cells, or both using techniques known in the art for purifying proteins including ion-exchange chromatography, gel filtration chromatography, ultrafiltration, electrophoresis, and immunoaffinity purification with antibodies specific for such peptide. In a preferred embodiment, the recombinant polypeptide is a fusion protein containing a domain which facilitates its purification, such as GST fusion protein.

Moreover, it will be generally appreciated that, under certain circumstances, it may be advantageous to provide homologs of one of the subject polypeptides which function in a limited capacity as one of either an agonist (mimetic) or an antagonist, in order to promote or inhibit only a subset of the biological activities of the naturally occurring form of the protein. Thus, specific biological effects can be elicited by treatment with a homolog of limited function, and with fewer side effects relative to treatment with agonists or antagonists which are directed to all of the biological activities of naturally occurring forms of subject proteins.

Homologs of each of the subject polypeptide can be generated by mutagenesis, such as by discrete point mutation(s), or by truncation. For instance, mutation can give rise to homologs which retain substantially the same, or merely a subset, of the biological activity of the polypeptide from which it was derived. Alternatively, antagonistic forms of the polypeptide can be generated which are able to inhibit the function of the naturally occurring form of the protein, such as by competitively binding to a receptor.

The recombinant polypeptides of the present invention also include homologs of the wild-type proteins, such as versions of those proteins which are resistant to proteolytic cleavage, for example, due to mutations which alter ubiquitination or other enzymatic targeting associated with the protein.

Polypeptides may also be chemically modified to create derivatives by forming covalent or aggregate conjugates with other chemical moieties, such as glycosyl groups, lipids, phosphate, acetyl groups and the like. Covalent derivatives of proteins can be prepared by linking the chemical moieties to functional groups on amino acid sidechains of the protein or at the N-terminus or at the C-terminus of the polypeptide.

Modification of the structure of the subject polypeptides can be for such purposes as enhancing therapeutic or prophylactic efficacy, stability (e.g., ex vivo shelf life and resistance to proteolytic degradation), or post-translational modifications (e.g., to alter phosphorylation pattern of protein). Such modified peptides, when designed to retain at least one activity of the naturally occurring form of the protein, or to produce specific antagonists thereof, are considered functional equivalents of the polypeptides described in more detail herein. Such modified peptides can be produced, for instance, by amino acid substitution, deletion, or addition. The substitutional variant may be a substituted conserved amino acid or a substituted non-conserved amino acid.

For example, it is reasonable to expect that an isolated replacement of a leucine with an isoleucine or valine, an aspartate with a glutamate, a threonine with a serine, or a similar replacement of an amino acid with a structurally related amino acid (i.e., isosteric and/or isoelectric mutations) will not have a major effect on the biological activity of the resulting molecule. Conservative replacements are those that take place within a family of amino acids that are related in their side chains. Genetically encoded amino acids can be divided into four families: (1) acidic=aspartate, glutamate; (2) basic=lysine, arginine, histidine; (3) nonpolar=alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and (4) uncharged polar=glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine. In similar fashion, the amino acid repertoire can be grouped as (1) acidic=aspartate, glutamate; (2) basic=lysine, arginine histidine, (3) aliphatic=glycine, alanine, valine, leucine, isoleucine, serine, threonine, with serine and threonine optionally be grouped separately as aliphatic-hydroxyl; (4) aromatic=phenylalanine, tyrosine, tryptophan; (5) amide=asparagine, glutamine; and (6) sulfur-containing=cysteine and methionine. (see, for example, Biochemistry, 2 nd ed., Ed. by L. Stryer, W H Freeman and Co.: 1981). Whether a change in the amino acid sequence of a peptide results in a functional homolog (e.g., functional in the sense that the resulting polypeptide mimics or antagonizes the wild-type form) can be readily determined by assessing the ability of the variant peptide to produce a response in cells in a fashion similar to the wild-type protein, or competitively inhibit such a response. Polypeptides in which more than one replacement has taken place can readily be tested in the same manner. The variant may be designed so as to retain biological activity of a particular region of the protein. In a non-limiting example, Osawa et al., 1994 , Biochemistry and Molecular International 34:1003-1009, discusses the actin binding region of a protein from several different species. The actin binding regions of the these species are considered homologous based on the fact that they have amino acids that fall within “homologous residue groups.” Homologous residues are judged according to the following groups (using single letter amino acid designations): STAG; ILVMF; HRK; DEQN; and FYW. For example, an S, a T, an A or a G can be in a position and the function (in this case actin binding) is retained.

Additional guidance on amino acid substitution is available from studies of protein evolution. Go et al., 1980 , Int. J. Peptide Protein Res. 15:211-224, classified amino acid residue sites as interior or exterior depending on their accessibility. More frequent substitution on exterior sites was confirmed to be general in eight sets of homologous protein families regardless of their biological functions and the presence or absence of a prosthetic group. Virtually all types of amino acid residues had higher mutabilities on the exterior than in the interior. No correlation between mutability and polarity was observed of amino acid residues in the interior and exterior, respectively. Amino acid residues were classified into one of three groups depending on their polarity: polar (Arg, Lys, His, Gln, Asn, Asp, and Glu); weak polar (Ala, Pro, Gly, Thr, and Ser), and nonpolar (Cys, Val, Met, Ile, Leu, Phe, Tyr, and Trp). Amino acid replacements during protein evolution were very conservative: 88% and 76% of them in the interior or exterior, respectively, were within the same group of the three. Inter-group replacements are such that weak polar residues are replaced more often by nonpolar residues in the interior and more often by polar residues on the exterior.

Querol et al., 1996 , Prot. Eng. 9:265-271, provides general rules for amino acid substitutions to enhance protein thermostability. New glycosylation sites can be introduced as discussed in Olsen and Thomsen, 1991 , J. Gen. Microbiol. 137:579-585. An additional disulfide bridge can be introduced, as discussed by Perry and Wetzel, 1984 , Science 226:555-557; Pantoliano et al., 1987 , Biochemistry 26:2077-2082; Matsumura et al., 1989 , Nature 342:291-293; Nishikawa et al., 1990 , Protein Eng. 3:443-448; Takagi et al., 1990 , J. Biol. Chem. 265:6874-6878; Clarke et al., 1993 , Biochemistry 32:4322-4329; and Wakarchuk et al., 1994 , Protein Eng. 7:1379-1386.

An additional metal binding site can be introduced, according to Toma et al., 1991 , Biochemistry 30:97-106, and Haezerbrouck et al., 1993 , Protein Eng. 6:643-649. Substitutions with prolines in loops can be made according to Masul et al., 1994 , Appl. Env. Microbiol. 60:3579-3584; and Hardy et al., FEBS Lett. 317:89-92.

Cysteine-depleted muteins are considered variants within the scope of the invention. These variants can be constructed according to methods disclosed in U.S. Pat. No. 4,959,314, which discloses how to substitute other amino acids for cysteines, and how to determine biological activity and effect of the substitution. Such methods are suitable for proteins according to this invention that have cysteine residues suitable for such substitutions, for example to eliminate disulfide bond formation.

To learn the identity and function of the gene that correlates with an nucleic acid, the nucleic acids or corresponding amino acid sequences can be screened against profiles of protein families. Such profiles focus on common structural motifs among proteins of each family. Publicly available profiles are described above. Additional or alternative profiles are described below.

In comparing a new nucleic acid with known sequences, several alignment tools are available. Examples include PileUp, which creates a multiple sequence alignment, and is described in Feng et al., J. Mol. Evol. ( 1987) 25:351-360. Another method, GAP, uses the alignment method of Needleman et al., J. Mol. Biol. (1970) 48:443-453. GAP is best suited for global alignment of sequences. A third method, BestFit, functions by inserting gaps to maximize the number of matches using the local homology algorithm of Smith and Waterman, Adv. Appl. Math. (1981) 2:482-489.

Examples of such profiles are described below.

Chemokines

Chemokines are a family of proteins that have been implicated in lymphocyte trafficking, inflammatory diseases, angiogenesis, hematopoiesis, and viral infection. See, for example, Rollins, Blood (1997) 90(3):909-928, and Wells et al., J. Leuk. Biol. (1997) 61:545-550. U.S. Pat. No. 5,605,817 discloses DNA encoding a chemokine expressed in fetal spleen. U.S. Pat. No. 5,656,724 discloses chemokine-like proteins and methods of use. U.S. Pat. No. 5,602,008 discloses DNA encoding a chemokine expressed by liver.

Mutants of the encoded chemokines are polypeptides having an amino acid sequence that possesses at least one amino acid substitution, addition, or deletion as compared to native chemokines. Fragments possess the same amino acid sequence of the native chemokines; mutants may lack the amino and/or carboxyl terminal sequences. Fusions are mutants, fragments, or the native chemokines that also include amino and/or carboxyl terminal amino acid extensions.

The number or type of the amino acid changes is not critical, nor is the length or number of the amino acid deletions, or amino acid extensions that are incorporated in the chemokines as compared to the native chemokine amino acid sequences. A polynucleotide encoding one of these variant polypeptides will retain at least about 80% amino acid identity with at least one known chemokine. Preferably, these polypeptides will retain at least about 85% amino acid sequence identity, more preferably, at least about 90%; even more preferably, at least about 95%. In addition, the variants will exhibit at least 80%; preferably about 90%; more preferably about 95% of at least one activity exhibited by a native chemokine. Chemokine activity includes immunological, biological, receptor binding, and signal transduction functions of the native chemokine.

Chemotaxis. Assays for chemotaxis relating to neutrophils are described in Walz et al., Biochem. Biophys. Res. Commun. (1987) 149:755, Yoshimura et al., Proc. Natl. Acad. Sci. ( USA ) (1987) 84:9233, and Schroder et al., J. Immunol. (1987) 139:3474; to lymphocytes, Larsen et al., Science (1989) 243:1464, Carr et al., Proc. Natl. Acad. Sci. ( USA ) (1994) 91:3652; to tumor-infiltrating lymphocytes, Liao et al., J. Exp. Med (1995). 182:1301; to hemopoietic progenitors, Aiuti et al., J. Exp. Med. (1997) 185:111; to monocytes, Valente et al., Biochem. (1988) 27:4162; and to natural killer cells, Loetscher et al., J. Immunol. (1996) 156:322, and Allavena et al., Eur. J. Immunol. (1994) 24:3233.

Assays for determining the biological activity of attracting cosinophils are described in Dahinden et al., J. Exp. Med. (1994) 179:751, Weber et al., J. Immunol. (1995) 154:4166, and Noso et al., Biochem. Biophys. Res. Commun. (1994) 200:1470; for attracting dendritic cells, Sozzani et al., J. Immunol. (1995) 155:3292; for attracting basophils, in Dahinden et al., J. Exp. Med. (1994) 179:751, Alam et al., J. Immunol. (1994) 152:1298, Alam et al., J. Exp. Med. (1992) 176:781; and for activating neutrophils, Maghazaci et al., Eur. J. Immunol. (1996) 26:315, and Taub et al., J. Immunol. (1995) 155:3877. Native chemokines can act as mitogens for fibroblasts, assayed as described in Mullenbach et al., J. Biol Chem. (1986) 261:719.

Receptor Binding. Native chemokines exhibit binding activity with a number of receptors. Description of such receptors and assays to detect binding are described in, for example, Murphy et al., Science (1991) 253:1280; Combadiere et al., J. Biol. Chem. (1995) 270:29671; Daugherty et al., J. Exp. Med. (1996) 183:2349; Samson et al., Biochem. (1996) 35:3362; Raport et al., J. Biol. Chem. (1996) 271:17161; Combadiere et al., J. Leukoc. Biol. (1996) 60:147; Baba et al., J. Biol. Chem. (1997) 23:14893; Yosida et al., J. Biol. Chem. (1997) 272:13803; Arvannitakis et al., Nature (1997) 385:347, and many other assays are known in the art.

Kinase Activiation. Assays for kinase activation are described by Yen et al., J. Leukoc. Biol. (1997) 61:529; Dubois et al., J. Immunol. (1996) 156:1356; Turner et al., J. Immunol. (1995) 155:2437. Assays for inhibition of angiogenesis or cell proliferation are described in Maione et al., Science (1990) 247:77. Glycosaminoglycan production can be induced by native chemokines, assayed as described in Castor et al., Proc. Natl. Acad. Sci. ( USA ) (1983) 80:765. Chemokine-mediated histamine release from basophils is assayed as described in Dahinden et al., J. Exp. Med. (1989) 170:1787; and White et al., Immunol. Lett. (1989) 22:151. Heparin binding is described in Luster et al., J. Exp. Med. (1995) 182:219.

Dimerization Activity. Chemokines can possess dimerization activity, which can be assayed according to Burrows et al., Biochem. (1994) 33:12741; and Zhang et al., Mol. Cell. Biol. (1995) 15:4851. Native chemokines can play a role in the inflammatory response of viruses. This activity can be assayed as described in Bleul et al., Nature (1996) 382:829; and Oberlin et al., Nature (1996) 382:833. Exocytosis of monocytes can be promoted by native chemokines. The assay for such activity is described in Uguccioni et al., Eur. J. Immunol. (1995) 25:64. Native chemokines also can inhibit hemapoietic stem cell proliferation. The method for testing for such activity is reported in Graham et al., Nature (1990) 344:442.

Death Domain Proteins Several protein families contain death domain motifs (Feinstein and Kimchi, TIBS Letters (1995) 20:242-244). Some death domain-containing proteins are implicated in cytotoxic intracellular signaling (Cleveland and Ihle, Cell (1995) 81:479-482, Pan et al, Science (1997) 276:111-113, Duan and Dixit, Nature (1997) 385:86-89, and Chinnaiyan et al, Science (1996) 274:990-992). U.S. Pat. No. 5,563,039 describes a protein homologous to TRADD (Tumor Necrosis Factor Receptor-1 Associated Death Domain containing protein), and modifications of the active domain of TRADD that retain the functional characteristics of the protein, as well as apoptosis assays for testing the function of such death domain containing proteins. U.S. Pat. No. 5,658,883 discloses biologically active TGF-B1 peptides. U.S. Pat. No. 5,674,734 discloses protein RIP which contains a C-terminal death domain and an N-terminal kinase domain.

Leukemia Inhibitory Factor (LIF) An LIF profile is constructed from sequences of leukemia inhibitor factor, CT-1 (cardiotrophin-1), CNTF (ciliary neurotrophic factor), OSM (oncostatin M), and IL-6 (interleukin-6). This profile encompasses a family of secreted cytokines that have pleiotropic effects on many cell types including hepatocytes, osteoclasts, neuronal cells and cardiac myocytes, and can be used to detect additional genes encoding such proteins. These molecules are all structurally related and share a common co-receptor gp130 which mediates intracellular signal transduction by cytoplasmic tyrosine kinases such as src.

Novel proteins related to this family are also likely to be secreted, to activate gp130 and to function in the development of a variety of cell types. Thus new members of this family would be candidates to be developed as growth or survival factors for the cell types that they stimulate. For more details on this family of cytokines, see Pennica et al, Cytokine and Growth Factor Reviews (1996) 7:81-91. U.S. Pat. No. 5,420,247 discloses LIF receptor and fusion proteins. U.S. Pat. No. 5,443,825 discloses human LIF.

Angiopoietin Angiopoietin-1 is a secreted ligand of the TIE-2 tyrosine kinase; it functions as an angiogenic factor critical for normal vascular development. Angiopoietin-2 is a natural antagonist of angiopoietin-1and thus functions as an anti-angiogenic factor. These two proteins are structurally similar and activate the same receptor. (Folkman and D'Amore, Cell (1996) 87:1153-1155, and Davis et al., Cell (1996) 87:1161-1169.)

The angiopoietin molecules are composed of two domains, a coiled-coil region and a region related to fibrinogen. The fibrinogen domain is found in many molecules including ficolin and tesascin, and is well defined structurally with many members.

Receptor Protein-Tyrosine Kinases Receptor Protein-Tyrosine Kinases or RPTKs are described in Lindberg, Annu. Rev. Cell Biol. (1994) 10:251-337.

Growth Factors: Epidermal Growth Factor (EGF) and Fibroblast Growth Factor (FGF) For a discussion of growth factor superfamilies, see Growth Factors: A Practical Approach , Appendix A1 (Ed. McKay and Leigh, Oxford University Press, NY, 1993) pp. 237-243.

The alignments (pretty box) for EGF and FGF are shown in FIGS. 1 and 2 , respectively. U.S. Pat. No. 4,444,760 discloses acidic brain fibroblast growth factor, which is active in the promotion of cell division and wound healing. U.S. Pat. No. 5,439,818 discloses DNA encoding human recombinant basic fibroblast growth factor, which is active in wound healing. U.S. Pat. No. 5,604,293 discloses recombinant human basic fibroblast growth factor, which is useful for wound healing. U.S. Pat. No. 5,410,832 discloses brain-derived and recombinant acidic fibroblast growth factor, which act as mitogens for mesoderm and neuroectoderm-derived cells in culture, and promote wound healing in soft tissue, cartilaginous tissue and musculo-skeletal tissue. U.S. Pat. No. 5,387,673 discloses biologically active fragments of FGF that retain activity.

Proteins of the TNF Family A profile derived from the TNF family is created by aligning sequences of the following TNF family members: nerve growth factor (NGF), lymphotoxin, Fas ligand, tumor necrosis factor (TNF ), CD40 ligand, TRAIL, ox40 ligand, 4-1BB ligand, CD27 ligand, and CD30 ligand. The profile is designed to identify sequences of proteins that constitute new members or homologues of this family of proteins.

U.S. Pat. No. 5,606,023 discloses mutant TNF proteins; U.S. Pat. No. 5,597,899 and U.S. Pat. No. 5,486,463 disclose TNF muteins; and U.S. Pat. No. 5,652,353 discloses DNA encoding TNF-α muteins.

Members of the TNF family of proteins have been show in vitro to multimerize, as described in Burrows et al., Biochem. (1994) 33:12741 and Zhang et al., Mol. Cell. Biol. (1995) 154851 and bind receptors as described in Browning et al., J. Immunol. (1994) 147:1230, Androlewicz et al., J. Biol. Chem .(1992) 267:2542, and Crowe et al., Science (1994) 264:707.

In vivo, TNFs proteolytically cleave a target protein as described in Kriegel et al., Cell (1988) 53:45 and Mohler et al., Nature (1994) 370:218 and demonstrate cell proliferation and differentiation activity. T-cell or thymocyte proliferation is assayed as described in Armitage et al., Eur. J. Immunol. (1992) 22:447; Current Protocols in Immunology, ed. J. E. Coligan et al., 3.1-3.19; Takai et al., J. Immunol. (1986) 137:3494-3500, Bertagnoli et al., J. Immunol. (1990) 145:1706-1712, Bertagnoli et al., J. Immunol. (1991) 133:327-340, Bertagnoli et al., J. Immunol. (1992) 149:3778-3783, and Bowman et al., J. Immunol. (1994) 152:1756-1761. B cell proliferation and Ig secretion are assayed as described in Maliszewski, J. Immunol. (1990) 144:3028-3033, and Assays for B Cell Function: In vitro antibody production, Mond and Brunswick, Current Protocols in Immunol., Coligan Ed vol 1 pp 3.8.1-3.8.16, John Wiley and Sons, Toronto 1994, Kehrl et al., Science (1987) 238:1144 and Boussiotis et al., PNAS USA (1994) 91:7007.

Other in vivo activities include upregulation of cell surface antigens, upregulation of costimulatory molecules, and cellular aggregation/adhesion as described in Barrett et al., J. Immunol. (1991) 146:1722; Bjorck et al., Eur. J. Immunol. (1993) 23:1771; Clark et al., Annu Rev. Immunol. (1991) 9:97; Ranheim et al., J. Exp. Med. (1994) 177:925; Yellin, J. Immunol. (1994) 153:666; and Gruss et al., Blood (1994) 84:2305.

Proliferation and differentiation of hematopoietic and lymphopoietic cells has also been shown in vivo for TNFs, using assays for embryonic differentiation and hematopoiesis as described in Johansson et al., Cellular Biology (1995) 15:141-151, Keller et al., Mol. Cell. Biol. (1993) 13:473-486, McClanahan et al., Blood (1993) 81:2903-2915 and using assays to detect stem cell survival and differentiation as described in Culture of Hematopoietic Cells, Freshney et al. eds, pp 1-21, 23-29, 139-162, 163-179, and 265-268, Wiley-Liss, Inc., New York, N.Y., 1994, and Hirajama et al., PNAS USA (1992) 89:5907-5911.

In vivo activities of TNFs also include lymphocyte survival and apoptosis, assayed as described in Darzynkewicz et al., Cytometry (1992) 13:795-808; Gorczca et al., Leukemia (1993) 7:659-670; Itoh et al., Cell (1991) 66:233-243; Zacharduk, J. Immunol. (1990) 145:4037-4045; Zamai et al., Cytometry (1993) 14:891-897; and Gorczyca et al., Int'l J. Oncol. (1992) 1:639-648.

Some members of the TNF family are cleaved from the cell surface; others remain membrane bound. The three-dimensional structure of TNF is discussed in Sprang and Eck, Tumor Necrosis Factors; supra.

TNF proteins include a transmembrane domain. The protein is cleaved into a shorter soluble version, as described in Kriegler et al., Cell (1988) 53:45-53, Perez et al., Cell (1990) 63:251-258, and Shaw et al., Cell (1986) 46:659-667. The transmembrane domain is between amino acid 46 and 77 and the cytoplasmic domain is between position 1 and 45 on the human form of TNFα&Parenopenst;. The 3-dimensional motifs of TNF include a sandwich of two pleated β-sheets. Each sheet is composed of anti-parallel α-strands. α-Strands facing each other on opposite sites of the sandwich are connected by short polypeptide loops, as described in Van Ostade et al., Protein Engineering (1994) 7(1):5-22, and Sprang et al., Tumor Necrosis Factors; supra.

Residues of the TNF family proteins that are involved in the β-sheet secondary structure have been identified as described in Van Ostade et al., Protein Engineering (1994) 7(1):5-22, and Sprang et al., Tumor Necrosis Factors; supra.

TNF receptors are disclosed in U.S. Pat. No. 5,395,760. A profile derived from the TNF receptor family is created by aligning sequences of the TNF receptor family, including Apo1/Fas, TNFR I and II, death receptor3 (DR3), CD40, ox40, CD27, and CD30. Thus, the profile is designed to identify, from the nucleic acids of the invention, sequences of proteins that constitute new members or homologs of this family of proteins.

Tumor necrosis factor receptors exist in two forms in humans: p55 TNFR and p75 TNFR, both of which provide intracellular signals upon binding with a ligand. The extracellular domains of these receptor proteins are cysteine rich. The receptors can remain membrane bound, although some forms of the receptors are cleaved forming soluble receptors. The regulation, diagnostic, prognostic, and therapeutic value of soluble TNF receptors is discussed in Aderka, Cytokine and Growth Factor Reviews, (1996) 7(3):231-240.

PDGF Family U.S. Pat. No. 5,326,695 discloses platelet derived growth factor agonists; bioactive portions of PDGF-B are used as agonists. U.S. Pat. No. 4,845,075 discloses biologically active B-chain homodimers, and also includes variants and derivatives of the PDGF-B chain. U.S. Pat. No. 5,128,321 discloses PDGF analogs and methods of use. Proteins having the same bioactivity as PDGF are disclosed, including A and B chain proteins.

Kinase (Including MKK) Family U.S. Pat. No. 5,650,501 discloses serine/threonine kinase, associated with mitotic and meiotic cell division; the protein has a kinase domain in its N-terminal and 3 PEST regions in the C-terminus. U.S. Pat. No. 5,605,825 discloses human PAK65, a serine protein kinase.

The foregoing discussion provides a few examples of the protein profiles that can be compared with the nucleic acids of the invention. One skilled in the art can use these and other protein profiles to identify the genes that correlate with the nucleic acids.

IX. Determinin2 the Function of the Encoded Expression Products

Ribozymes, antisense constructs, dominant negative mutants, and triplex formation can be used to determine function of the expression product of an nucleic acid-related gene.

A. Ribozymes

Trans-cleaving catalytic RNAs (ribozymes) are RNA molecules possessing endoribonuclease activity. Ribozymes are specifically designed for a particular target, and the target message must contain a specific nucleotide sequence. They are engineered to cleave any RNA species site-specifically in the background of cellular RNA. The cleavage event renders the mRNA unstable and prevents protein expression. Importantly, ribozymes can be used to inhibit expression of a gene of unknown function for the purpose of determining its function in an in vitro or in vivo context, by detecting the phenotypic effect.

One commonly used ribozyme motif is the hammerhead, for which the substrate sequence requirements are minimal. Design of the hammerhead ribozyme is disclosed in Usman et al., Current Opin. Strict. Biol. (1996) 6:527-533. Usman also discusses the therapeutic uses of ribozymes. Ribozymes can also be prepared and used as described in Long et al., FASEB J. (1993) 7:25; Symons, Ann. Rev. Biochem. (1992) 61:641; Perrotta et al., Biochem. (1992) 31:16-17; Ojwang et al., Proc. Natl. Acad. Sci. ( USA ) (1992) 89:10802-10806; and U.S. Pat. No. 5,254,678. Ribozyme cleavage of HIV-I RNA is described in U.S. Pat. No. 5,144,019; methods of cleaving RNA using ribozymes is described in U.S. Pat. No. 5,116,742; and methods for increasing the specificity of ribozymes are described in U.S. Pat. No. 5,225,337 and Koizumi et al., Nucleic Acid Res. (1989) 17:7059-7071. Preparation and use of ribozyme fragments in a hammerhead structure are also described by Koizumi et al., Nucleic Acids Res. (1989) 17:7059-7071. Preparation and use of ribozyme fragments in a hairpin structure are described by Chowrira and Burke, Nucleic Acids Res. (1992) 20:2835. Ribozymes can also be made by rolling transcription as described in Daubendiek and Kool, Nat. Biotechnol. (1997) 15(3):273-277.

The hybridizing region of the ribozyme may be modified or may be prepared as a branched structure as described in Horn and Urdea, Nucleic Acids Res. (1989) 17:6959-67. The basic structure of the ribozymes may also be chemically altered in ways familiar to those skilled in the art, and chemically synthesized ribozymes can be administered as synthetic oligonucleotide derivatives modified by monomeric units. In a therapeutic context, liposome mediated delivery of ribozymes improves cellular uptake, as described in Birikh et al., Eur. J. Biochem. (1997) 245:1-16.

Using the nucleic acid sequences of the invention and methods known in the art, ribozymes are designed to specifically bind and cut the corresponding mRNA species. Ribozymes thus provide a means to inhibit the expression of any of the proteins encoded by the disclosed nucleic acids or their full-length genes. The full-length gene need not be known in order to design and use specific inhibitory ribozymes. In the case of a nucleic acid or cDNA of unknown function, ribozymes corresponding to that nucleotide sequence can be tested in vitro for efficacy in cleaving the target transcript. Those ribozymes that effect cleavage in vitro are further tested in vivo. The ribozyme can also be used to generate an animal model for a disease, as described in Birikh et al., Eur. J. Biochem. (1997) 245:1-16. An effective ribozyme is used to determine the function of the gene of interest by blocking its transcription and detecting a change in the cell. Where the gene is found to be a mediator in a disease, an effective ribozyme is designed and delivered in a gene therapy for blocking transcription and expression of the gene.

Therapeutic and functional genomic applications of ribozymes proceed beginning with knowledge of a portion of the coding sequence of the gene to be inhibited. Thus, for many genes, a partial nucleic acid sequence provides adequate sequence for constructing an effective ribozyme. A target cleavage site is selected in the target sequence, and a ribozyme is constructed based on the 5′ and 3′ nucleotide sequences that flank the cleavage site. Retroviral vectors are engineered to express monomeric and multimeric hammerhead ribozymes targeting the mRNA of the target coding sequence. These monomeric and multimeric ribozymes are tested in vitro for an ability to cleave the target mRNA. A cell line is stably transduced with the retroviral vectors expressing the ribozymes, and the transduction is confirmed by Northern blot analysis and reverse-transcription polymerase chain reaction (RT-PCR). The cells are screened for inactivation of the target mRNA by such indicators as reduction of expression of disease markers or reduction of the gene product of the target mRNA.

B. Antisense

Antisense nucleic acids are designed to specifically bind to RNA, resulting in the formation of RNA-DNA or RNA-RNA hybrids, with an arrest of DNA replication, reverse transcription or messenger RNA translation. Antisense polynucleotides based on a selected nucleic acid sequence can interfere with expression of the corresponding gene. Antisense polynucleotides are typically generated within the cell by expression from antisense constructs that contain the antisense nucleic acid strand as the transcribed strand. Antisense nucleic acids will bind and/or interfere with the translation of nucleic acid-related mRNA. The expression products of control cells and cells treated with the antisense construct are compared to detect the protein product of the gene corresponding to the nucleic acid. The protein is isolated and identified using routine biochemical methods.

One rationale for using antisense methods to determine the function of the gene corresponding to a nucleic acid is the biological activity of antisense therapeutics. Antisense therapy for a variety of cancers is in clinical phase and has been discussed extensively in the literature. Reed reviewed antisense therapy directed at the Bc1-2 gene in tumors; gene transfer-mediated overexpression of Bcl-2 in tumor cell lines conferred resistance to many types of cancer drugs. (Reed, J. C., N.C.I. (1997) 89:988-990). The potential for clinical development of antisense inhibitors of ras is discussed by Cowsert, L. M., Anti - Cancer Drug Design (1997) 12:359-371. Additional important antisense targets include leukemia (Geurtz, A. M., Anti - Cancer Drug Design (1997) 12:341-358); human C-ref kinase (Monia, B. P., Anti - Cancer Drug Design (1997) 12:327-339); and protein kinase C (McGraw et al., Anti - Cancer Drug Design (1997) 12:315-326.

Given the extensive background literature and clinical experience in antisense therapy, one skilled in the art can use selected nucleic acids of the invention as additional potential therapeutics. The choice of nucleic acid can be narrowed by first testing them for binding to “hot spot” regions of the genome of cancerous cells. If a nucleic acid is identified as binding to a “hot spot”, testing the nucleic acid as an antisense compound in the corresponding cancer cells clearly is warranted.

Ogunbiyi et al., Gastroenterology (1997) 113(3):761-766 describe prognostic use of allelic loss in colon cancer; Barks et al., Genes, Chromosomes, and Cancer (1997) 19(4):278-285 describe increased chromosome copy number detected by FISH in malignant melanoma; Nishizake et al., Genes, Chromosomes, and Cancer (1997) 19(4):267-272 describe genetic alterations in primary breast cancer and their metastases and direct comparison using modified comparative genome hybridization; and Elo et al., Cancer Research (1997) 57(16):3356-3359 disclose that loss of heterozygosity at 16z24.1-q24.2 is significantly associated with metastatic and aggressive behavior of prostate cancer.

C. Dominant Negative Mutations

As an alternative method for identifying function of the nucleic acid-related gene, dominant negative mutations are readily generated for corresponding proteins that are active as homomultimers. A mutant polypeptide will interact with wild-type polypeptides (made from the other allele) and form a non-functional multimer. Thus, a mutation is in a substrate-binding domain, a catalytic domain, or a cellular localization domain. Preferably, the mutant polypeptide will be overproduced. Point mutations are made that have such an effect. In addition, fusion of different polypeptides of various lengths to the terminus of a protein can yield dominant negative mutants. General strategies are available for making dominant negative mutants. See Herskowitz, Nature (1987) 329:219-222. Such a technique can be used for creating a loss-of-function mutation, which is useful for determining the function of a protein.

D. Triplex Formation

Endogenous gene expression can also be reduced by inactivating or “knocking out” the gene or its promoter using targeted homologous recombination. (E.g., see Smithies et al., 1985, Nature 317:230-234; Thomas & Capecchi, 1987, Cell 51:503-512; Thompson et al., 1989 Cell 5:313-321; each of which is incorporated by reference herein in its entirety). For example, a mutant, non-functional gene (or a completely unrelated DNA sequence) flanked by DNA homologous to the endogenous gene (either the coding regions or regulatory regions of the gene) can be used, with or without a selectable marker and/or a negative selectable marker, to transfect cells that express that gene in vivo. Insertion of the DNA construct, via targeted homologous recombination, results in inactivation of the gene.

Alternatively, endogenous gene expression can be reduced by targeting deoxyribonucleotide sequences complementary to the regulatory region of the target gene (i.e., the gene promoter and/or enhancers) to form triple helical structures that prevent transcription of the gene in target cells in the body. (See generally, Helene, C. 1991, Anticancer Drug Des., 6(6):569-84; Helene, C., et al., 1992, Ann, N.Y. Accad. Sci., 660:27-36; and Maher, L. J., 1992, Bioassays 14(12):807-15).

Nucleic acid molecules to be used in triple helix formation for the inhibition of transcription are preferably single stranded and composed of deoxyribonucleotides. The base composition of these oligonucleotides should promote triple helix formation via Hoogsteen base-pairing rules, which generally require sizable stretches of either purines or pyrimidines to be present on one strand of a duplex. Nucleotide sequences may be pyrimidine-based, which will result in TAT and CGC triplets across the three associated strands of the resulting triple helix. The pyrimidine-rich molecules provide base complementarity to a purine-rich region of a single strand of the duplex in a parallel orientation to that strand. In addition, nucleic acid molecules may be chosen that are purine-rich, for example, containing a stretch of G residues. These molecules will form a triple helix with a DNA duplex that is rich in GC pairs, in which the majority of the purine residues are located on a single strand of the targeted duplex, resulting in CGC triplets across the three strands in the triplex.

Alternatively, the potential sequences that can be targeted for triple helix formation may be increased by creating a so called “switchback” nucleic acid molecule. Switchback molecules are synthesized in an alternating 5′-3′, 3′-5′ manner, such that they base pair with first one strand of a duplex and then the other, eliminating the necessity for a sizable stretch of either purines or pyrimidines to be present on one strand of a duplex.

Antisense RNA and DNA, ribozyme, and triple helix molecules of the invention may be prepared by any method known in the art for the synthesis of DNA and RNA molecules. These include techniques for chemically synthesizing oligodeoxyribonucleotides and oligoribonucleotides well known in the art such as for example solid phase phosphoramidite chemical synthesis. Alternatively, RNA molecules may be generated by in vitro and in vivo transcription of DNA sequences encoding the antisense RNA molecule. Such DNA sequences may be incorporated into a wide variety of vectors which incorporate suitable RNA polymerase promoters such as the T7 or SP6 polymerase promoters. Alternatively, antisense cDNA constructs that synthesize antisense RNA constitutively or inducibly, depending on the promoter used, can be introduced stably into cell lines.

Moreover, various well known modifications to nucleic acid molecules may be introduced as a means of increasing intracellular stability and half-life. Possible modifications include but are not limited to the addition of flanking sequences of ribonucleotides or deoxyribonucleotides to the 5′ and/or 3′ ends of the molecule or the use of phosphorothioate or 2′ O-methyl rather than phosphodiesterase linkages within the oligodeoxyribonucleotide backbone.

X. Diagnostic & Prognostic Assays and Drug Screening Methods

The present invention provides method for determining whether a subject is at risk for developing a disease or condition characterized by unwanted cell proliferation by detecting the disclosed biomarkers, i.e., the disclosed nucleic acid markers (SEQ ID Nos: 1-544) and/or polypeptide markers for colon cancer encoded thereby.

In clinical applications, human tissue samples can be screened for the presence and/or absence of the biomarkers identified herein. Such samples could consist of needle biopsy cores, surgical resection samples, lymph node tissue, or serum. For example, these methods include obtaining a biopsy, which is optionally fractionated by cryostat sectioning to enrich tumor cells to about 80% of the total cell population. In certain embodiments, nucleic acids extracted from these samples may be amplified using techniques well known in the art. The levels of selected markers detected would be compared with statistically valid groups of metastatic, non-metastatic malignant, benign, or normal colon tissue samples.

In one embodiment, the diagnostic method comprises determining whether a subject has an abnormal mRNA and/or protein level of the disclosed markers, such as by Northern blot analysis, reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, immunoprecipitation, Western blot hybridization, or immunohistochemistry. According to the method, cells are obtained from a subject and the levels of the disclosed biomarkers, protein or mRNA level, is determined and compared to the level of these markers in a healthy subject. An abnormal level of the biomarker polypeptide or mRNA levels is likely to be indicative of cancer such as colon cancer.

Accordingly, in one aspect, the invention provides probes and primers that are specific to the unique nucleic acid markers disclosed herein. Accordingly, the nucleic acid probes comprise a nucleotide sequence at least 12 nucleotides in length, preferably at least 15 nucleotides, more preferably, 25 nucleotides, and most preferably at least 40 nucleotides, and up to all or nearly all of the coding sequence which is complementary to a portion of the coding sequence of a marker nucleic acid sequence, which nucleic acid sequence is represented by SEQ ID Nos: 1-544 or a sequence complementary thereto.

In one embodiment, the method comprises using a nucleic acid probe to determine the presence of cancerous cells in a tissue from a patient. Specifically, the method comprises:

1. providing a nucleic acid probe comprising a nucleotide sequence at least 12 nucleotides in length, preferably at least 15 nucleotides, more preferably, 25 nucleotides, and most preferably at least 40 nucleotides, and up to all or nearly all of the coding sequence which is complementary to a portion of the coding sequence of a nucleic acid sequence represented by SEQ ID Nos: 1-544 or a sequence complementary thereto and is differentially expressed in tumors cells, such as colon cancer cells;

2. obtaining a tissue sample from a patient potentially comprising cancerous cells;

3. providing a second tissue sample containing cells substantially all of which are non-cancerous;

4. contacting the nucleic acid probe under stringent conditions with RNA of each of said first and second tissue samples (e.g., in a Northern blot or in situ hybridization assay); and

5. comparing (a) the amount of hybridization of the probe with RNA of the first tissue sample, with (b) the amount of hybridization of the probe with RNA of the second tissue sample; wherein a statistically significant difference in the amount of hybridization with the RNA of the first tissue sample as compared to the amount of hybridization with the RNA of the second tissue sample is indicative of the presence of cancerous cells in the first tissue sample.

In one aspect, the method comprises in situ hybridization with a probe derived from a given marker nucleic acid sequence, which nucleic acid sequence is represented by SEQ ID Nos: 1-544 or a sequence complementary thereto. The method comprises contacting the labeled hybridization probe with a sample of a given type of tissue potentially containing cancerous or pre-cancerous cells as well as normal cells, and determining whether the probe labels some cells of the given tissue type to a degree significantly different (e.g., by at least a factor of two, or at least a factor of five, or at least a factor of twenty, or at least a factor of fifty) than the degree to which it labels other cells of the same tissue type.

Also within the invention is a method of determining the phenotype of a test cell from a given human tissue, e.g., whether the cell is (a) normal, or (b) cancerous or precancerous, by contacting the mRNA of a test cell with a nucleic acid probe at least 12 nucleotides in length, preferably at least 15 nucleotides, more preferably at least 25 nucleotides, and most preferably at least 40 nucleotides, and up to all or nearly all of a sequence which is complementary to a portion of the coding sequence of a nucleic acid sequence represented by SEQ ID Nos: 1-544 or a sequence complementary thereto, and which is differentially expressed in tumor cells as compared to normal cells of the given tissue type; and determining the approximate amount of hybridization of the probe to the mRNA, an amount of hybridization either more or less than that seen with the mRNA of a normal cell of that tissue type being indicative that the test cell is cancerous or pre-cancerous.

Alternatively, the above diagnostic assays may be carried out using antibodies to detect the protein product encoded by the marker nucleic acid sequence, which nucleic acid sequence is represented by SEQ ID Nos: 1-544 or a sequence complementary thereto. Accordingly, in one embodiment, the assay would include contacting the proteins of the test cell with an antibody specific for the gene product of a nucleic acid represented by SEQ ID Nos: 1-544 or a sequence complementary thereto, the marker nucleic acid being one which is expressed at a given control level in normal cells of the same tissue type as the test cell, and determining the approximate amount of immunocomplex formation by the antibody and the proteins of the test cell, wherein a statistically significant difference in the amount of the immunocomplex formed with the proteins of a test cell as compared to a normal cell of the same tissue type is an indication that the test cell is cancerous or pre-cancerous.

Another such method includes the steps of: providing an antibody specific for the gene product of a marker nucleic acid sequence represented by SEQ ID Nos 1-544, the gene product being present in cancerous tissue of a given tissue type (e.g., colon tissue) at a level more or less than the level of the gene product in non-cancerous tissue of the same tissue type; obtaining from a patient a first sample of tissue of the given tissue type, which sample potentially includes cancerous cells; providing a second sample of tissue of the same tissue type (which may be from the same patient or from a normal control, e.g. another individual or cultured cells), this second sample containing normal cells and essentially no cancerous cells; contacting the antibody with protein (which may be partially purified, in lysed but unfractionated cells, or in situ) of the first and second samples under conditions permitting immunocomplex formation between the antibody and the marker nucleic acid sequence product present in the samples; and comparing (a) the amount of immunocomplex formation in the first sample, with (b) the amount of immunocomplex formation in the second sample, wherein a statistically significant difference in the amount of immunocomplex formation in the first sample less as compared to the amount of immunocomplex formation in the second sample is indicative of the presence of cancerous cells in the first sample of tissue.

The subject invention further provides a method of determining whether a cell sample obtained from a subject possesses an abnormal amount of marker polypeptide which comprises (a) obtaining a cell sample from the subject, (b) quantitatively determining the amount of the marker polypeptide in the sample so obtained, and (c) comparing the amount of the marker polypeptide so determined with a known standard, so as to thereby determine whether the cell sample obtained from the subject possesses an abnormal amount of the marker polypeptide. Such marker polypeptides may be detected by immunohistochemical assays, dot-blot assays, ELISA and the like.

Immunoassays are commonly used to quantitate the levels of proteins in cell samples, and many other immunoassay techniques are known in the art. The invention is not limited to a particular assay procedure, and therefore is intended to include both homogeneous and heterogeneous procedures. Exemplary immunoassays which can be conducted according to the invention include fluorescence polarization immunoassay (FPIA), fluorescence immunoassay (FIA), enzyme immunoassay (EIA), nephelometric inhibition immunoassay (NIA), enzyme linked immunosorbent assay (ELISA), and radioimmunoassay (RIA). An indicator moiety, or label group, can be attached to the subject antibodies and is selected so as to meet the needs of various uses of the method which are often dictated by the availability of assay equipment and compatible immunoassay procedures. General techniques to be used in performing the various immunoassays noted above are known to those of ordinary skill in the art.

In another embodiment, the level of the encoded product, i.e., the product encoded by SEQ ID Nos 1-544 or a sequence complementary thereto, in a biological fluid (e.g., blood or urine) of a patient may be determined as a way of monitoring the level of expression of the marker nucleic acid sequence in cells of that patient. Such a method would include the steps of obtaining a sample of a biological fluid from the patient, contacting the sample (or proteins from the sample) with an antibody specific for a encoded marker polypeptide, and determining the amount of immune complex formation by the antibody, with the amount of immune complex formation being indicative of the level of the marker encoded product in the sample. This determination is particularly instructive when compared to the amount of immune complex formation by the same antibody in a control sample taken from a normal individual or in one or more samples previously or subsequently obtained from the same person.

In another embodiment, the method can be used to determine the amount of marker polypeptide present in a cell, which in turn can be correlated with progression of a hyperproliferative disorder, e.g., colon cancer. The level of the marker polypeptide can be used predictively to evaluate whether a sample of cells contains cells which are, or are predisposed towards becoming, transformed cells. Moreover, the subject method can be used to assess the phenotype of cells which are known to be transformed, the phenotyping results being useful in planning a particular therapeutic regimen. For instance, very high levels of the marker polypeptide in sample cells is a powerful diagnostic and prognostic marker for a cancer, such as colon cancer. The observation of marker polypeptide level can be utilized in decisions regarding, e.g., the use of more aggressive therapies.

As set out above, one aspect of the present invention relates to diagnostic assays for determining, in the context of cells isolated from a patient, if the level of a marker polypeptide is significantly reduced in the sample cells. The term “significantly reduced” refers to a cell phenotype wherein the cell possesses a reduced cellular amount of the marker polypeptide relative to a normal cell of similar tissue origin. For example, a cell may have less than about 50%, 25%, 10%, or 5% of the marker polypeptide that a normal control cell. In particular, the assay evaluates the level of marker polypeptide in the test cells, and, preferably, compares the measured level with marker polypeptide detected in at least one control cell, e.g., a normal cell and/or a transformed cell of known phenotype.

Of particular importance to the subject invention is the ability to quantitate the level of marker polypeptide as determined by the number of cells associated with a normal or abnormal marker polypeptide level. The number of cells with a particular marker polypeptide phenotype may then be correlated with patient prognosis. In one embodiment of the invention, the marker polypeptide phenotype of the lesion is determined as a percentage of cells in a biopsy which are found to have abnormally high/low levels of the marker polypeptide. Such expression may be detected by immunohistochemical assays, dot-blot assays, ELISA and the like.

Where tissue samples are employed, immunohistochemical staining may be used to determine the number of cells having the marker polypeptide phenotype. For such staining, a multiblock of tissue is taken from the biopsy or other tissue sample and subjected to proteolytic hydrolysis, employing such agents as protease K or pepsin. In certain embodiments, it may be desirable to isolate a nuclear fraction from the sample cells and detect the level of the marker polypeptide in the nuclear fraction.

The tissue samples are fixed by treatment with a reagent such as formalin, glutaraldehyde, methanol, or the like. The samples are then incubated with an antibody, preferably a monoclonal antibody, with binding specificity for the marker polypeptides. This antibody may be conjugated to a label for subsequent detection of binding. Samples are incubated for a time sufficient for formation of the immuno-complexes. Binding of the antibody is then detected by virtue of a label conjugated to this antibody. Where the antibody is unlabeled, a second labeled antibody may be employed, e.g., which is specific for the isotype of the anti-marker polypeptide antibody. Examples of labels which may be employed include radionuclides, fluorescers, chemiluminescers, enzymes and the like.

Where enzymes are employed, the substrate for the enzyme may be added to the samples to provide a colored or fluorescent product. Examples of suitable enzymes for use in conjugates include horseradish peroxidase, alkaline phosphatase, malate dehydrogenase and the like. Where not commercially available, such antibody-enzyme conjugates are readily produced by techniques known to those skilled in the art.

In one embodiment, the assay is performed as a dot blot assay. The dot blot assay finds particular application where tissue samples are employed as it allows determination of the average amount of the marker polypeptide associated with a single cell by correlating the amount of marker polypeptide in a cell-free extract produced from a predetermined number of cells.

It is well established in the cancer literature that tumor cells of the same type (e.g., breast and/or colon tumor cells) may not show uniformly increased expression of individual oncogenes or uniformly decreased expression of individual tumor suppressor genes. There may also be varying levels of expression of a given marker gene even between cells of a given type of cancer, further emphasizing the need for reliance on a battery of tests rather than a single test. Accordingly, in one aspect, the invention provides for a battery of tests utilizing a number of probes of the invention, in order to improve the reliability and/or accuracy of the diagnostic test.

In one embodiment, the present invention also provides a method wherein nucleic acid probes are immobilized on a DNA chip in an organized array. Oligonucleotides can be bound to a solid support by a variety of processes, including lithography. For example a chip can hold up to 250,000 oligonucleotides (GeneChip, Affymetrix). These nucleic acid probes comprise a nucleotide sequence at least about 12 nucleotides in length, preferably at least about 15 nucleotides, more preferably at least about 25 nucleotides, and most preferably at least about 40 nucleotides, and up to all or nearly all of a sequence which is complementary to a portion of the coding sequence of a marker nucleic acid sequence represented by SEQ ID Nos: 1-544 and is differentially expressed in tumor cells, such as colon cancer cells. The present invention provides significant advantages over the available tests for various cancers, such as colon cancer, because it increases the reliability of the test by providing an array of nucleic acid markers on a single chip.

The method includes obtaining a biopsy, which is optionally fractionated by cryostat sectioning to enrich tumor cells to about 80% of the total cell population. The DNA or RNA is then extracted, amplified, and analyzed with a DNA chip to determine the presence of absence of the marker nucleic acid sequences.

In one embodiment, the nucleic acid probes are spotted onto a substrate in a two-dimensional matrix or array. Samples of nucleic acids can be labeled and then hybridized to the probes. Double-stranded nucleic acids, comprising the labeled sample nucleic acids bound to probe nucleic acids, can be detected once the unbound portion of the sample is washed away.

The probe nucleic acids can be spotted on substrates including glass, nitrocellulose, etc. The probes can be bound to the substrate by either covalent bonds or by non-specific interactions, such as hydrophobic interactions. The sample nucleic acids can be labeled using radioactive labels, fluorophores, chromophores, etc.

Techniques for constructing arrays and methods of using these arrays are described in EP No. 0 799 897; PCT No. WO 97/29212; PCT No. WO 97/27317; EP No. 0 785 280; PCT No. WO 97/02357; U.S. Pat. No. 5,593,839; U.S. Pat. No. 5,578,832; EP No. 0 728 520; U.S. Pat. No. 5,599,695; EP No. 0 721 016; U.S. Pat. No. 5,556,752; PCT No. WO 95/22058; and U.S. Pat. No. 5,631,734.

Further, arrays can be used to examine differential expression of genes and can be used to determine gene function. For example, arrays of the instant nucleic acid sequences can be used to determine if any of the nucleic acid sequences are differentially expressed between normal cells and cancer cells, for example. High expression of a particular message in a cancer cell, which is not observed in a corresponding normal cell, can indicate a cancer specific protein.

In yet another embodiment, the invention contemplates using a panel of antibodies which are generated against the marker polypeptides of this invention, which polypeptides are encoded by SEQ ID Nos: 1-544. Such a panel of antibodies may be used as a reliable diagnostic probe for colon cancer. The assay of the present invention comprises contacting a biopsy sample containing cells, e.g., colon cells, with a panel of antibodies to one or more of the encoded products to determine the presence or absence of the marker polypeptides.

The diagnostic methods of the subject invention may also be employed as follow-up to treatment, e.g., quantitation of the level of marker polypeptides may be indicative of the effectiveness of current or previously employed cancer therapies as well as the effect of these therapies upon patient prognosis.

Accordingly, the present invention makes available diagnostic assays and reagents for detecting gain and/or loss of marker polypeptides from a cell in order to aid in the diagnosis and phenotyping of proliferative disorders arising from, for example, tumorigenic transformation of cells.

The diagnostic assays described above can be adapted to be used as prognostic assays, as well. Such an application takes advantage of the sensitivity of the assays of the invention to events which take place at characteristic stages in the progression of a tumor. For example, a given marker gene may be up- or downregulated at a very early stage, perhaps before the cell is irreversibly committed to developing into a malignancy, while another marker gene may be characteristically up or down regulated only at a much later stage. Such a method could involve the steps of contacting the mRNA of a test cell with a nucleic acid probe derived from a given marker nucleic acid which is expressed at different characteristic levels in cancerous or precancerous cells at different stages of tumor progression, and determining the approximate amount of hybridization of the probe to the mRNA of the cell, such amount being an indication of the level of expression of the gene in the cell, and thus an indication of the stage of tumor progression of the cell; alternatively, the assay can be carried out with an antibody specific for the gene product of the given marker nucleic acid, contacted with the proteins of the test cell. A battery of such tests will disclose not only the existence and location of a tumor, but also will allow the clinician to select the mode of treatment most appropriate for the tumor, and to predict the likelihood of success of that treatment.

The methods of the invention can also be used to follow the clinical course of a tumor. For example, the assay of the invention can be applied to a tissue sample from a patient; following treatment of the patient for the cancer, another tissue sample is taken and the test repeated. Successful treatment will result in either removal of all cells which demonstrate differential expression characteristic of the cancerous or precancerous cells, or a substantial increase in expression of the gene in those cells, perhaps approaching or even surpassing normal levels.

In yet another embodiment, the invention provides methods for determining whether a subject is at risk for developing a disease, such as a predisposition to develop cancer, for example colon cancer, associated with an aberrant activity of any one of the polypeptides encoded by nucleic acids of SEQ ID Nos: 1-544, wherein the aberrant activity of the polypeptide is characterized by detecting the presence or absence of a genetic lesion characterized by at least one of (i) an alteration affecting the integrity of a gene encoding a marker polypeptides, or (ii) the mis-expression of the encoding nucleic acid. To illustrate, such genetic lesions can be detected by ascertaining the existence of at least one of (i) a deletion of one or more nucleotides from the nucleic acid sequence, (ii) an addition of one or more nucleotides to the nucleic acid sequence, (iii) a substitution of one or more nucleotides of the nucleic acid sequence, (iv) a gross chromosomal rearrangement of the nucleic acid sequence, (v) a gross alteration in the level of a messenger RNA transcript of the nucleic acid sequence, (vii) aberrant modification of the nucleic acid sequence, such as of the methylation pattern of the genomic DNA, (vii) the presence of a non-wild type splicing pattern of a messenger RNA transcript of the gene, (viii) a non-wild type level of the marker polypeptide, (ix) allelic loss of the gene, and/or (x) inappropriate post-translational modification of the marker polypeptide.

The present invention provides assay techniques for detecting lesions in the encoding nucleic acid sequence. These methods include, but are not limited to, methods involving sequence analysis, Southern blot hybridization, restriction enzyme site mapping, and methods involving detection of absence of nucleotide pairing between the nucleic acid to be analyzed and a probe.

Specific diseases or disorders, e.g., genetic diseases or disorders, are associated with specific allelic variants of polymorphic regions of certain genes, which do not necessarily encode a mutated protein. Thus, the presence of a specific allelic variant of a polymorphic region of a gene in a subject can render the subject susceptible to developing a specific disease or disorder. Polymorphic regions in genes, can be identified, by determining the nucleotide sequence of genes in populations of individuals. If a polymorphic region is identified, then the link with a specific disease can be determined by studying specific populations of individuals, e.g, individuals which developed a specific disease, such as colon cancer. A polymorphic region can be located in any region of a gene, e.g., exons, in coding or non coding regions of exons, introns, and promoter region.

In an exemplary embodiment, there is provided a nucleic acid composition comprising a nucleic acid probe including a region of nucleotide sequence which is capable of hybridizing to a sense or antisense sequence of a gene or naturally occurring mutants thereof, or 5′ or 3′ flanking sequences or intronic sequences naturally associated with the subject genes or naturally occurring mutants thereof. The nucleic acid of a cell is rendered accessible for hybridization, the probe is contacted with the nucleic acid of the sample, and the hybridization of the probe to the sample nucleic acid is detected. Such techniques can be used to detect lesions or allelic variants at either the genomic or mRNA level, including deletions, substitutions, etc., as well as to determine mRNA transcript levels.

A preferred detection method is allele specific hybridization using probes overlapping the mutation or polymorphic site and having about 5, 10, 20, 25, or 30 nucleotides around the mutation or polymorphic region. In a preferred embodiment of the invention, several probes capable of hybridizing specifically to allelic variants are attached to a solid phase support, e.g., a “chip”. Mutation detection analysis using these chips comprising oligonucleotides, also termed “DNA probe arrays” is described e.g., in Cronin et al. (1996) Human Mutation 7:244. In one embodiment, a chip comprises all the allelic variants of at least one polymorphic region of a gene. The solid phase support is then contacted with a test nucleic acid and hybridization to the specific probes is detected. Accordingly, the identity of numerous allelic variants of one or more genes can be identified in a simple hybridization experiment.

In certain embodiments, detection of the lesion comprises utilizing the probe/primer in a polymerase chain reaction (PCR) (see, e.g. U.S. Pat. Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or, alternatively, in a ligase chain reaction (LCR) (see, e.g., Landegran et al. (1988) Science 241:1077-1080; and Nakazawa et al. (1994) PNAS 91:360-364), the latter of which an be particularly useful for detecting point mutations in the gene (see Abravaya et al. (1995) Nuc Acid Res 23:675-682). In a merely illustrative embodiment, the method includes the steps of (i) collecting a sample of cells from a patient, (ii) isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, (iii) contacting the nucleic acid sample with one or more primers which specifically hybridize to a nucleic acid sequence under conditions such that hybridization and amplification of the nucleic acid (if present) occurs, and (iv) detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. It is anticipated that PCR and/or LCR may be desirable to use as a preliminary amplification step in conjunction with any of the techniques used for detecting mutations described herein.

Alternative amplification methods include: self sustained sequence replication (Guatelli, J. C. et al., 1990, Proc. Natl. Acad. Sci. USA 87:1874-1878), transcriptional amplification system (Kwoh, D. Y. et al., 1989, Proc. Natl. Acad. Sci. USA 86:1173-1177), Q-Beta Replicase (Lizardi, P. M. et al., 1988, Bio/Technology 6:1197), or any other nucleic acid amplification method, followed by the detection of the amplified molecules using techniques well known to those of skill in the art. These detection schemes are especially useful for the detection of nucleic acid molecules if such molecules are present in very low numbers.

In a preferred embodiment of the subject assay, mutations in, or allelic variants, of a gene from a sample cell are identified by alterations in restriction enzyme cleavage patterns. For example, sample and control DNA is isolated, amplified (optionally), digested with one or more restriction endonucleases, and fragment length sizes are determined by gel electrophoresis. Moreover, the use of sequence specific ribozymes (see, for example, U.S. Pat. No. 5,498,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site.

Another aspect of the invention is directed to the identification of agents capable of modulating the differentiation and proliferation of cells characterized by aberrant proliferation. In this regard, the invention provides assays for determining compounds that modulate the expression of the marker nucleic acids (SEQ ID Nos: 1-544) and/or alter for example, inhibit the bioactivity of the encoded polypeptide.

Several in vivo methods can be used to identify compounds that modulate expression of the marker nucleic acids (SEQ ID Nos: 1-544) and/or alter for example, inhibit the bioactivity of the encoded polypeptide.

Drug screening is performed by adding a test compound to a sample of cells, and monitoring the effect. A parallel sample which does not receive the test compound is also monitored as a control. The treated and untreated cells are then compared by any suitable phenotypic criteria, including but not limited to microscopic analysis, viability testing, ability to replicate, histological examination, the level of a particular RNA or polypeptide associated with the cells, the level of enzymatic activity expressed by the cells or cell lysates, and the ability of the cells to interact with other cells or compounds. Differences between treated and untreated cells indicates effects attributable to the test compound.

Desirable effects of a test compound include an effect on any phenotype that was conferred by the cancer-associated marker nucleic acid sequence. Examples include a test compound that limits the overabundance of mRNA, limits production of the encoded protein, or limits the functional effect of the protein. The effect of the test compound would be apparent when comparing results between treated and untreated cells.

The invention thus also encompasses methods of screening for agents which inhibit expression of the nucleic acid markers (SEQ ID Nos: 1-544) in vitro, comprising exposing a cell or tissue in which the marker nucleic acid mRNA is detectable in cultured cells to an agent in order to determine whether the agent is capable of inhibiting production of the mRNA; and determining the level of mRNA in the exposed cells or tissue, wherein a decrease in the level of the mRNA after exposure of the cell line to the agent is indicative of inhibition of the marker nucleic acid mRNA production.

Alternatively, the screening method may include in vitro screening of a cell or tissue in which marker protein is detectable in cultured cells to an agent suspected of inhibiting production of the marker protein; and determining the level of the marker protein in the cells or tissue, wherein a decrease in the level of marker protein after exposure of the cells or tissue to the agent is indicative of inhibition of marker protein production.

The invention also encompasses in vivo methods of screening for agents which inhibit expression of the marker nucleic acids, comprising exposing a mammal having tumor cells in which marker mRNA or protein is detectable to an agent suspected of inhibiting production of marker mRNA or protein; and determining the level of marker mRNA or protein in tumor cells of the exposed mammal. A decrease in the level of marker mRNA or protein after exposure of the mammal to the agent is indicative of inhibition of marker nucleic acid expression.

Accordingly, the invention provides a method comprising incubating a cell expressing the marker nucleic acids (SEQ ID Nos: 1-544) with a test compound and measuring the mRNA or protein level. The invention further provides a method for quantitatively determining the level of expression of the marker nucleic acids in a cell population, and a method for determining whether an agent is capable of increasing or decreasing the level of expression of the marker nucleic acids in a cell population. The method for determining whether an agent is capable of increasing or decreasing the level of expression of the marker nucleic acids in a cell population comprises the steps of (a) preparing cell extracts from control and agent-treated cell populations, (b) isolating the marker polypeptides from the cell extracts, (c) quantifying (e.g., in parallel) the amount of an immunocomplex formed between the marker polypeptide and an antibody specific to said polypeptide. The marker polypeptides of this invention may also be quantified by assaying for its bioactivity. Agents that induce increased the marker nucleic acid expression may be identified by their ability to increase the amount of immunocomplex formed in the treated cell as compared with the amount of the immunocomplex formed in the control cell. In a similar manner, agents that decrease expression of the marker nucleic acid may be identified by their ability to decrease the amount of the immunocomplex formed in the treated cell extract as compared to the control cell.

mRNA levels can be determined by Northern blot hybridization. mRNA levels can also be determined by methods involving PCR. Other sensitive methods for measuring mRNA, which can be used in high throughput assays, e.g., a method using a DELFIA endpoint detection and quantification method, are described, e.g., in Webb and Hurskainen (1996) Journal of Biomolecular Screening 1:119. Marker protein levels can be determined by immunoprecipitations or immunohistochemistry using an antibody that specifically recognizes the protein product encoded by SEQ ID Nos: 1-544.

Agents that are identified as active in the drug screening assay are candidates to be tested for their capacity to block cell proliferation activity. These agents would be useful for treating a disorder involving aberrant growth of cells, especially colon cells.

A variety of assay formats will suffice and, in light of the present disclosure, those not expressly described herein will nevertheless be comprehended by one of ordinary skill in the art. For instance, the assay can be generated in many different formats, and include assays based on cell-free systems, e.g., purified proteins or cell lysates, as well as cell-based assays which utilize intact cells.

In many drug screening programs which test libraries of compounds and natural extracts, high throughput assays are desirable in order to maximize the number of compounds surveyed in a given period of time. Assays of the present invention which are performed in cell-free systems, such as may be derived with purified or semi-purified proteins or with lysates, are often preferred as “primary” screens in that they can be generated to permit rapid development and relatively easy detection of an alteration in a molecular target which is mediated by a test compound. Moreover, the effects of cellular toxicity and/or bioavailability of the test compound can be generally ignored in the in vitro system, the assay instead being focused primarily on the effect of the drug on the molecular target as may be manifest in an alteration of binding affinity with other proteins or changes in enzymatic properties of the molecular target.

A. Use of Nucleic Acids as Probes in Mapping and in Tissue Profiling Probes

Polynucleotide probes as described above, e.g., comprising at least 12 contiguous nucleotides selected from the nucleotide sequence of an nucleic acid as shown in SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, are used for a variety of purposes, including identification of human chromosomes and determining transcription levels. Additional disclosure about preferred regions of the nucleic acid sequences is found in the accompanying tables.

The nucleotide probes are labeled, for example, with a radioactive, fluorescent, biotinylated, or chemiluminescent label, and detected by well known methods appropriate for the particular label selected. Protocols for hybridizing nucleotide probes to preparations of metaphase chromosomes are also well known in the art. A nucleotide probe will hybridize specifically to nucleotide sequences in the chromosome preparations which are complementary to the nucleotide sequence of the probe. A probe that hybridizes specifically to a nucleic acid should provide a detection signal at least 5-, 10-, or 20-fold higher than the background hybridization provided with other unrelated sequences.

In a non-limiting example, commercial programs are available for identifying regions of chromosomes commonly associated with disease, such as cancer. Nucleic acids of the invention can be used to probe these regions. For example, if, through profile searching, a nucleic acid is identified as corresponding to a gene encoding a kinase, its ability to bind to a cancer-related chromosomal region will suggest its role as a kinase in one or more stages of tumor cell development/growth. Although some experimentation would be required to elucidate the role, the nucleic acid constitutes a new material for isolating a specific protein that has potential for developing a cancer diagnostic or therapeutic.

Nucleotide probes are used to detect expression of a gene corresponding to the nucleic acid. For example, in Northern blots, mRNA is separated electrophoretically and contacted with a probe. A probe is detected as hybridizing to an mRNA species of a particular size. The amount of hybridization is quantitated to determine relative amounts of expression, for example under a particular condition. Probes are also used to detect products of amplification by polymerase chain reaction. The products of the reaction are hybridized to the probe and hybrids are detected. Probes are used for in situ hybridization to cells to detect expression. Probes can also be used in vivo for diagnostic detection of hybridizing sequences. Probes are typically labeled with a radioactive isotope. Other types of detectable labels may be used such as chromophores, fluorophores, and enzymes.

Expression of specific mRNA can vary in different cell types and can be tissue specific. This variation of mRNA levels in different cell types can be exploited with nucleic acid probe assays to determine tissue types. For example, PCR, branched DNA probe assays, or blotting techniques utilizing nucleic acid probes substantially identical or complementary to nucleic acids of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, can determine the presence or absence of target cDNA or mRNA.

Examples of a nucleotide hybridization assay are described in Urdea et al., PCT WO92/02526 and Urdea et al., U.S. Pat. No. 5,124,246, both incorporated herein by reference. The references describe an example of a sandwich nucleotide hybridization assay.

Alternatively, the Polymerase Chain Reaction (PCR) is another means for detecting small amounts of target nucleic acids, as described in Mullis et al., Meth. Enzymol. (1987) 155:335-350; U.S. Pat. No. 4,683,195; and U.S. Pat. No. 4,683,202, all incorporated herein by reference. Two primer polynucleotides nucleotides hybridize with the target nucleic acids and are used to prime the reaction. The primers may be composed of sequence within or 3′ and 5′ to the polynucleotides of the Sequence Listing. Alternatively, if the primers are 3′ and 5′ to these polynucleotides, they need not hybridize to them or the complements. A thermostable polymerase creates copies of target nucleic acids from the primers using the original target nucleic acids as a template. After a large amount of target nucleic acids is generated by the polymerase, it is detected by methods such as Southern blots. When using the Southern blot method, the labeled probe will hybridize to a polynucleotide of the Sequence Listing or complement.

Furthermore, mRNA or cDNA can be detected by traditional blotting techniques described in Sambrook et al., “Molecular Cloning: A Laboratory Manual” (New York, Cold Spring Harbor Laboratory, 1989). mRNA or cDNA generated from mRNA using a polymerase enzyme can be purified and separated using gel electrophoresis. The nucleic acids on the gel are then blotted onto a solid support, such as nitrocellulose. The solid support is exposed to a labeled probe and then washed to remove any unhybridized probe. Next, the duplexes containing the labeled probe are detected. Typically, the probe is labeled with radioactivity.

Mapping

Nucleic acids of the present invention are used to identify a chromosome on which the corresponding gene resides. Using fluorescence in situ hybridization (FISH) on normal metaphase spreads, comparative genomic hybridization allows total genome assessment of changes in relative copy number of DNA sequences. See Schwartz and Samad, Current Opinions in Biotechnology (1994) 8:70-74; Kallioniemi et al., Seminars in Cancer Biology (1993) 4:41-46; Valdes and Tagle, Methods in Molecular Biology (1997) 68:1, Boultwood, ed., Human Press, Totowa, N.J.

Preparations of human metaphase chromosomes are prepared using standard cytogenetic techniques from human primary tissues or cell lines. Nucleotide probes comprising at least 12 contiguous nucleotides selected from the nucleotide sequence of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, are used to identify the corresponding chromosome. The nucleotide probes are labeled, for example, with a radioactive, fluorescent, biotinylated, or chemiluminescent label, and detected by well known methods appropriate for the particular label selected. Protocols for hybridizing nucleotide probes to preparations of metaphase chromosomes are also well known in the art. A nucleotide probe will hybridize specifically to nucleotide sequences in the chromosome preparations that are complementary to the nucleotide sequence of the probe. A probe that hybridizes specifically to a target gene provides a detection signal at least 5-, 10-, or 20-fold higher than the background hybridization provided with unrelated coding sequences.

Nucleic acids are mapped to particular chromosomes using, for example, radiation hybrids or chromosome-specific hybrid panels. See Leach et al, Advances in Genetics, (1995) 33:63-99; Walter et al., Nature Genetics (1994) 7:22-28; Walter and Goodfellow, Trends in Genetics (1992) 9:352. Panels for radiation hybrid mapping are available from Research Genentics, Inc., Huntsville, Alabama, USA. Databases for markers using various panels are available via the world wide web at http:/F/shgc-www.stanford.edu; and other locations. The statistical program RHMAP can be used to construct a map based on the data from radiation hybridization with a measure of the relative likelihood of one order versus another. RHMAP is available via the world wide web at http://www.sph.umich.edu/group/statgen/software.

Such mapping can be useful in identifying the function of the target gene by its proximity to other genes with known function. Function can also be assigned to the target gene when particular syndromes or diseases map to the same chromosome.

Tissue Profiling

The nucleic acids of the present invention can be used to determine the tissue type from which a given sample is derived. For example, a metastatic lesion is identified by its developmental organ or tissue source by identifying the expression of a particular marker of that organ or tissue. If a nucleic acid is expressed only in a specific tissue type, and a metastatic lesion is found to express that nucleic acid, then the developmental source of the lesion has been identified. Expression of a particular nucleic acid is assayed by detection of either the corresponding mRNA or the protein product. Immunological methods, such as antibody staining, are used to detect a particular protein product. Hybridization methods may be used to detect particular mRNA species, including but not limited to in situ hybridization and Northern blotting.

Use of Polymorphisms

A nucleic acid will be useful in forensics, genetic analysis, mapping, and diagnostic applications if the corresponding region of a gene is polymorphic in the human population. A particular polymorphic form of the nucleic acid may be used to either identify a sample as deriving from a suspect or rule out the possibility that the sample derives from the suspect. Any means for detecting a polymorphism in a gene are used, including but not limited to electrophoresis of protein polymorphic variants, differential sensitivity to restriction enzyme cleavage, and hybridization to an allele-specific probe.

B. Use of Nucleic Acids and Encoded Polypeptides to Raise Antibodies

Expression products of a nucleic acid, the corresponding mRNA or cDNA, or the corresponding complete gene are prepared and used for raising antibodies for experimental, diagnostic, and therapeutic purposes. For nucleic acids to which a corresponding gene has not been assigned, this provides an additional method of identifying the corresponding gene. The nucleic acid or related cDNA is expressed as described above, and antibodies are prepared. These antibodies are specific to an epitope on the encoded polypeptide, and can precipitate or bind to the corresponding native protein in a cell or tissue preparation or in a cell-free extract of an in vitro expression system.

Immunogens for raising antibodies are prepared by mixing the polypeptides encoded by the nucleic acids of the present invention with adjuvants. Alternatively, polypeptides are made as fusion proteins to larger immunogenic proteins. Polypeptides are also covalently linked to other larger immunogenic proteins, such as keyhole limpet hemocyanin. Immunogens are typically administered intradermally, subcutaneously, or intramuscularly. Immunogens are administered to experimental animals such as rabbits, sheep, and mice, to generate antibodies. Optionally, the animal spleen cells are isolated and fused with myeloma cells to form hybridomas which secrete monoclonal antibodies. Such methods are well known in the art. According to another method known in the art, the nucleic acid is administered directly, such as by intramuscular injection, and expressed in vivo. The expressed protein generates a variety of protein-specific immune responses, including production of antibodies, comparable to administration of the protein.

Preparations of polyclonal and monoclonal antibodies specific for nucleic acid-encoded proteins and polypeptides are made using standard methods known in the art. The antibodies specifically bind to epitopes present in the polypeptides encoded by a nucleic acid of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto. In another embodiment, the antibodies specifically bind to epitopes present in a polypeptide encoded by SEQ ID Nos. 1-544. Typically, at least about 6, 8, 10, or 12 contiguous amino acids are required to form an epitope. However, epitopes which involve non-contiguous amino acids may require more, for example, at least about 15, 25, or 50 amino acids. A short sequence of a nucleic acid may then be unsuitable for use as an epitope to raise antibodies for identifying the corresponding novel protein, because of the potential for cross-reactivity with a known protein. However, the antibodies may be useful for other purposes, particularly if they identify common structural features of a known protein and a novel polypeptide encoded by a nucleic acid of the invention.

Antibodies that specifically bind to human nucleic acid-encoded polypeptides should provide a detection signal at least about 5-, 10-, or 20-fold higher than a detection signal provided with other proteins when used in Western blots or other immunochemical assays. Preferably, antibodies that specifically bind nucleic acid T-encoded polypeptides do not detect other proteins in immunochemical assays and can immunoprecipitate nucleic acid-encoded proteins from solution.

To test for the presence of serum antibodies to the nucleic acid-encoded polypeptide in a human population, human antibodies are purified by methods well known in the art. Preferably, the antibodies are affinity purified by passing antiserum over a column to which a nucleic acid-encoded protein, polypeptide, or fusion protein is bound. The bound antibodies can then be eluted from the column, for example using a buffer with a high salt concentration.

In addition to the antibodies discussed above, genetically engineered antibody derivatives are made, such as single chain antibodies.

Antibodies may be made by using standard protocols known in the art (See, for example, Antibodies: A Laboratory Manual ed. by Harlow and Lane (Cold Spring Harbor Press: 1988)). A mammal, such as a mouse, hamster, or rabbit can be immunized with an immunogenic form of the peptide (e.g., a mammalian polypeptide or an antigenic fragment which is capable of eliciting an antibody response, or a fusion protein as described above).

In one aspect, this invention includes monoclonal antibodies that show a subject polypeptide is highly expressed in colorectal tissue or tumor tissue, especially colon cancer tissue or colon cancer-derived cell lines. Therefore, in one embodiment, this invention provides a diagnostic tool for the analysis of expression of a subject polypeptide in general, and in particular, as a diagnostic for colon cancer.

Techniques for conferring immunogenicity on a protein or peptide include conjugation to carriers or other techniques well known in the art. An immunogenic portion of a protein can be administered in the presence of adjuvant. The progress of immunization can be monitored by detection of antibody titers in plasma or serum. Standard ELISA or other immunoassays can be used with the immunogen as antigen to assess the levels of antibodies. In a preferred embodiment, the subject antibodies are immunospecific for antigenic determinants of a protein of a mammal, e.g., antigenic determinants of a protein encoded by one of SEQ ID Nos. 1-544 or closely related homologs (e.g., at least 90% identical, and more preferably at least 95% identical).

Following immunization of an animal with an antigenic preparation of a polypeptide, antisera can be obtained and, if desired, polyclonal antibodies isolated from the serum. To produce monoclonal antibodies, antibody-producing cells (lymphocytes) can be harvested from an immunized animal and fused by standard somatic cell fusion procedures with immortalizing cells such as myeloma cells to yield hybridoma cells. Such techniques are well known in the art, and include, for example, the hybridoma technique (originally developed by Kohler and Milstein, (1975) Nature, 256: 495-497), the human B cell hybridoma technique (Kozbar et al., (1983) Immunology Today, 4: 72), and the EBV-hybridoma technique to produce human monoclonal antibodies (Cole et al., (1985) Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc. pp. 77-96). Hybridoma cells can be screened immunochemically for production of antibodies specifically reactive with a polypeptide of the present invention and monoclonal antibodies isolated from a culture comprising such hybridoma cells.

The term antibody as used herein is intended to include fragments thereof which are also specifically reactive with one of the subject polypeptides. Antibodies can be fragmented using conventional techniques and the fragments screened for utility in the same manner as described above for whole antibodies. For example, F(ab) 2 fragments can be generated by treating antibody with pepsin. The resulting F(ab) 2 fragment can be treated to reduce disulfide bridges to produce Fab fragments. The antibody of the present invention is further intended to include bispecific, single-chain, and chimeric and humanized molecules having affinity for a polypeptide conferred by at least one CDR region of the antibody. In preferred embodiments, the antibodies, the antibody further comprises a label attached thereto and able to be detected, (e.g., the label can be a radioisotope, fluorescent compound, chemiluminescent compound, enzyme, or enzyme co-factor).

Antibodies can be used, e.g., to monitor protein levels in an individual for determining, e.g., whether a subject has a disease or condition, such as colon cancer, associated with an aberrant protein level, or allowing determination of the efficacy of a given treatment regimen for an individual afflicted with such a disorder. The level of polypeptides may be measured from cells in bodily fluid, such as in blood samples.

Another application of antibodies of the present invention is in the immunological screening of cDNA libraries constructed in expression vectors such as gt11, gt18-23, ZAP, and ORF8. Messenger libraries of this type, having coding sequences inserted in the correct reading frame and orientation, can produce fusion proteins. For instance, gt11 will produce fusion proteins whose amino termini consist of β-galactosidase amino acid sequences and whose carboxyl termini consist of a foreign polypeptide. Antigenic epitopes of a protein, e.g., other orthologs of a particular protein or other paralogs from the same species, can then be detected with antibodies, as, for example, reacting nitrocellulose filters lifted from infected plates with antibodies. Positive phage detected by this assay can then be isolated from the infected plate. Thus, the presence of homologs can be detected and cloned from other animals, as can alternate isoforms (including splicing variants) from humans.

In another embodiment, a panel of monoclonal antibodies may be used, wherein each of the epitope's involved functions are represented by a monoclonal antibody. Loss or perturbation of binding of a monoclonal antibody in the panel would be indicative of a mutational attention of the protein and thus of the corresponding gene.

C. Differential Expression

The present invention also provides a method to identify abnormal or diseased tissue in a human. For nucleic acids corresponding to profiles of protein families as described above, the choice of tissue may be dictated by the putative biological function. The expression of a gene corresponding to a specific nucleic acid is compared between a first tissue that is suspected of being diseased and a second, normal tissue of the human. The normal tissue is any tissue of the human, especially those that express the target gene including, but not limited to, brain, thymus, testis, heart, prostate, placenta, spleen, small intestine, skeletal muscle, pancreas, and the mucosal lining of the colon.

The tissue suspected of being abnormal or diseased can be derived from a different tissue type of the human, but preferably it is derived from the same tissue type; for example an intestinal polyp or other abnormal growth should be compared with normal intestinal tissue. A difference between the target gene, mRNA, or protein in the two tissues which are compared, for example in molecular weight, amino acid or nucleotide sequence, or relative abundance, indicates a change in the gene, or a gene which regulates it, in the tissue of the human that was suspected of being diseased.

The target genes in the two tissues are compared by any means known in the art. For example, the two genes are sequenced, and the sequence of the gene in the tissue suspected of being diseased is compared with the gene sequence in the normal tissue. The target genes, or portions thereof, in the two tissues are amplified, for example using nucleotide primers based on the nucleotide sequence shown in the Sequence Listing, using the polymerase chain reaction. The amplified genes or portions of genes are hybridized to nucleotide probes selected from a corresponding nucleotide sequence shown SEQ ID No. 1-544. A difference in the nucleotide sequence of the target gene in the tissue suspected of being diseased compared with the normal nucleotide sequence suggests a role of the nucleic acid-encoded proteins in the disease, and provides a lead for preparing a therapeutic agent. The nucleotide probes are labeled by a variety of methods, such as radiolabeling, biotinylation, or labeling with fluorescent or chemiluminescent tags, and detected by standard methods known in the art.

Alternatively, target mRNA in the two tissues is compared. PolyA + RNA is isolated from the two tissues as is known in the art. For example, one of skill in the art can readily determine differences in the size or amount of target mRNA transcripts between the two tissues using Northern blots and nucleotide probes selected from the nucleotide sequence shown in the Sequence Listing. Increased or decreased expression of a target mRNA in a tissue sample suspected of being diseased, compared with the expression of the same target mRNA in a normal tissue, suggests that the expressed protein has a role in the disease, and also provides a lead for preparing a therapeutic agent.

Any method for analyzing proteins is used to compare two nucleic acid-encoded proteins from matched samples. The sizes of the proteins in the two tissues are compared, for example, using antibodies of the present invention to detect nucleic acid-encoded proteins in Western blots of protein extracts from the two tissues. Other changes, such as expression levels and subcellular localization, can also be detected immunologically, using antibodies to the corresponding protein. A higher or lower level of nucleic acid-encoded protein expression in a tissue suspected of being diseased, compared with the same nucleic acid-encoded protein expression level in a normal tissue, is indicative that the expressed protein has a role in the disease, and provides another lead for preparing a therapeutic agent.

Similarly, comparison of gene sequences or of gene expression products, e.g., mRNA and protein, between a human tissue that is suspected of being diseased and a normal tissue of a human, are used to follow disease progression or remission in the human. Such comparisons of genes, mRNA, or protein are made as described above.

For example, increased or decreased expression of the target gene in the tissue suspected of being neoplastic can indicate the presence of neoplastic cells in the tissue. The degree of increased expression of the target gene in the neoplastic tissue relative to expression of the gene in normal tissue, or differences in the amount of increased expression of the target gene in the neoplastic tissue over time, is used to assess the progression of the neoplasia in that tissue or to monitor the response of the neoplastic tissue to a therapeutic protocol over time.

The expression pattern of any two cell types can be compared, such as low and high metastatic tumor cell lines, or cells from tissue which have and have not been exposed to a therapeutic agent. A genetic predisposition to disease in a human is detected by comparing an target gene, mRNA, or protein in a fetal tissue with a normal target gene, mRNA, or protein. Fetal tissues that are used for this purpose include, but are not limited to, amniotic fluid, chorionic villi, blood, and the blastomere of an in vitro-fertilized embryo. The comparable normal target gene is obtained from any tissue. The mRNA or protein is obtained from a normal tissue of a human in which the target gene is expressed. Differences such as alterations in the nucleotide sequence or size of the fetal target gene or mRNA, or alterations in the molecular weight, amino acid sequence, or relative abundance of fetal target protein, can indicate a germline mutation in the target gene of the fetus, which indicates a genetic predisposition to disease.

D. Use of Nucleic Acids, and Encoded Polypeptides to Screen for Peptide Analogs and Antagonists

Polypeptides encoded by the instant nucleic acids, e.g., SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequence complementary thereto, and corresponding full length genes can be used to screen peptide libraries to identify binding partners, such as receptors, from among the encoded polypeptides.

A library of peptides may be synthesized following the methods disclosed in U.S. Pat. No. 5,010,175, and in PCT WO 91/17823. As described below in brief, one prepares a mixture of peptides, which is then screened to identify the peptides exhibiting the desired signal transduction and receptor binding activity. In the '175 method, a suitable peptide synthesis support (e.g., a resin) is coupled to a mixture of appropriately protected, activated amino acids. The concentration of each amino acid in the reaction mixture is balanced or adjusted in inverse proportion to its coupling reaction rate so that the product is an equimolar mixture of amino acids coupled to the starting resin. The bound amino acids are then deprotected, and reacted with another balanced amino acid mixture to form an equimolar mixture of all possible dipeptides. This process is repeated until a mixture of peptides of the desired length (e.g., hexamers) is formed. Note that one need not include all amino acids in each step: one may include only one or two amino acids in some steps (e.g., where it is known that a particular amino acid is essential in a given position), thus reducing the complexity of the mixture. After the synthesis of the peptide library is completed, the mixture of peptides is screened for binding to the selected polypeptide. The peptides are then tested for their ability to inhibit or enhance activity. Peptides exhibiting the desired activity are then isolated and sequenced.

The method described in WO 91/17823 is similar. However, instead of reacting the synthesis resin with a mixture of activated amino acids, the resin is divided into twenty equal portions (or into a number of portions corresponding to the number of different amino acids to be added in that step), and each amino acid is coupled individually to its portion of resin. The resin portions are then combined, mixed, and again divided into a number of equal portions for reaction with the second amino acid. In this manner, each reaction may be easily driven to completion. Additionally, one may maintain separate “subpools” by treating portions in parallel, rather than combining all resins at each step. This simplifies the process of determining which peptides are responsible for any observed receptor binding or signal transduction activity.

In such cases, the subpools containing, e.g., 1-2,000 candidates each are exposed to one or more polypeptides of the invention. Each subpool that produces a positive result is then resynthesized as a group of smaller subpools (sub-subpools) containing, e.g., 20-100 candidates, and reassayed. Positive sub-subpools may be resynthesized as individual compounds, and assayed finally to determine the peptides that exhibit a high binding constant. These peptides can be tested for their ability to inhibit or enhance the native activity. The methods described in WO 91/7823 and U.S. Pat. No. 5,194,392 (herein incorporated by reference) enable the preparation of such pools and subpools by automated techniques in parallel, such that all synthesis and resynthesis may be performed in a matter of days.

Peptide agonists or antagonists are screened using any available method, such as signal transduction, antibody binding, receptor binding, mitogenic assays, chemotaxis assays, etc. The methods described herein are presently preferred. The assay conditions ideally should resemble the conditions under which the native activity is exhibited in vivo, that is, under physiologic pH, temperature, and ionic strength. Suitable agonists or antagonists will exhibit strong inhibition or enhancement of the native activity at concentrations that do not cause toxic side effects in the subject. Agonists or antagonists that compete for binding to the native polypeptide may require concentrations equal to or greater than the native concentration, while inhibitors capable of binding irreversibly to the polypeptide may be added in concentrations on the order of the native concentration.

The end results of such screening and experimentation will be at least one novel polypeptide binding partner, such as a receptor, encoded by a nucleic acid of the invention, and at least one peptide agonist or antagonist of the novel binding partner. Such agonists and antagonists can be used to modulate, enhance, or inhibit receptor function in cells to which the receptor is native, or in cells that possess the receptor as a result of genetic engineering. Further, if the novel receptor shares biologically important characteristics with a known receptor, information about agonist/antagonist binding may help in developing improved agonists/antagonists of the known receptor.

E. Pharmaceutical Compositions and Therapeutic Uses

Pharmaceutical compositions can comprise polypeptides, antibodies, or polynucleotides of the claimed invention. The pharmaceutical compositions will comprise a therapeutically effective amount of either polypeptides, antibodies, or polynucleotides of the claimed invention.

The term “therapeutically effective amount” as used herein refers to an amount of a therapeutic agent to treat, ameliorate, or prevent a desired disease or condition, or to exhibit a detectable therapeutic or preventative effect. The effect can be detected by, for example, chemical markers or antigen levels. Therapeutic effects also include reduction in physical symptoms, such as decreased body temperature. The precise effective amount for a subject will depend upon the subject's size and health, the nature and extent of the condition, and the therapeutics or combination of therapeutics selected for administration. Thus, it is not useful to specify an exact effective amount in advance. However, the effective amount for a given situation can be determined by routine experimentation and is within the judgment of the clinician.

For purposes of the present invention, an effective dose will be from about 0.01 mg/ kg to 50 mg/kg or 0.05 mg/kg to about 10 mg/kg of the DNA constructs in the individual to which it is administered.

A pharmaceutical composition can also contain a pharmaceutically acceptable carrier. The term “pharmaceutically acceptable carrier” refers to a carrier for administration of a therapeutic agent, such as antibodies or a polypeptide, genes, and other therapeutic agents. The term refers to any pharmaceutical carrier that does not itself induce the production of antibodies harmful to the individual receiving the composition, and which may be administered without undue toxicity. Suitable carriers may be large, slowly metabolized macromolecules such as proteins, polysaccharides, polylactic acids, polyglycolic acids, polymeric amino acids, amino acid copolymers, and inactive virus particles. Such carriers are well known to those of ordinary skill in the art.

Pharmaceutically acceptable salts can be used therein, for example, mineral acid salts such as hydrochlorides, hydrobromides, phosphates, sulfates, and the like; and the salts of organic acids such as acetates, propionates, malonates, benzoates, and the like. A thorough discussion of pharmaceutically acceptable excipients is available in Remington's Pharmaceutical Sciences (Mack Pub. Co., N.J. 1991).

Pharmaceutically acceptable carriers in therapeutic compositions may contain liquids such as water, saline, glycerol and ethanol. Additionally, auxiliary substances, such as wetting or emulsifying agents, pH buffering substances, and the like, may be present in such vehicles. Typically, the therapeutic compositions are prepared as injectables, either as liquid solutions or suspensions; solid forms suitable for solution in, or suspension in, liquid vehicles prior to injection may also be prepared. Liposomes are included within the definition of a pharmaceutically acceptable carrier.

Delivery Methods

Once formulated, the nucleic acid compositions of the invention can be (1) administered directly to the subject; (2) delivered ex vivo, to cells derived from the subject; or (3) delivered in vitro for expression of recombinant proteins.

Direct delivery of the compositions will generally be accomplished by injection, either subcutaneously, intraperitoneally, intravenously or intramuscularly, or delivered to the interstitial space of a tissue. The compositions can also be administered into a tumor or lesion. Other modes of administration include oral and pulmonary administration, suppositories, and transdermal applications, needles, and gene guns or hyposprays. Dosage treatment may be a single dose schedule or a multiple dose schedule.

Methods for the ex vivo delivery and reimplantation of transformed cells into a subject are known in the art and described in e.g., International Publication No. WO 93/14778. Examples of cells useful in ex vivo applications include, for example, stem cells, particularly hematopoetic, lymph cells, macrophages, dendritic cells, or tumor cells.

Generally, delivery of nucleic acids for both ex vivo and in vitro applications can be accomplished by, for example, dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, and direct microinjection of the DNA into nuclei, all well known in the art.

Once a subject gene has been found to correlate with a proliferative disorder, such as neoplasia, dysplasia, and hyperplasia, the disorder may be amenable to treatment by administration of a therapeutic agent based on the nucleic acid or corresponding polypeptide.

Preparation of antisense polypeptides is discussed above. Neoplasias that are treated with the antisense composition include, but are not limited to, cervical cancers, melanomas, colorectal adenocarcinomas, Wilms' tumor, retinoblastoma, sarcomas, myosarcomas, lung carcinomas, leukemias, such as chronic myelogenous leukemia, promyelocytic leukemia, monocytic leukemia, and mycloid leukemia, and lymphomas, such as histiocytic lymphoma. Proliferative disorders that are treated with the therapeutic composition include disorders such as anhydric hereditary ectodermal dysplasia, congenital alveolar dysplasia, epithelial dysplasia of the cervix, fibrous dysplasia of bone, and mammary dysplasia. Hyperplasias, for example, endometrial, adrenal, breast, prostate, or thyroid hyperplasias or pseudoepitheliomatous hyperplasia of the skin, are treated with antisense therapeutic compositions. Even in disorders in which mutations in the corresponding gene are not implicated, downregulation or inhibition of nucleic acid-related gene expression can have therapeutic application. For example, decreasing nucleic acid-related gene expression can help to suppress tumors in which enhanced expression of the gene is implicated.

Both the dose of the antisense composition and the means of administration are determined based on the specific qualities of the therapeutic composition, the condition, age, and weight of the patient, the progression of the disease, and other relevant factors. Administration of the therapeutic antisense agents of the invention includes local or systemic administration, including injection, oral administration, particle gun or catheterized administration, and topical administration. Preferably, the therapeutic antisense composition contains an expression construct comprising a promoter and a polynucleotide segment of at least about 12, 22, 25, 30, or 35 contiguous nucleotides of the antisense strand of a nucleic acid. Within the expression construct, the polynucleotide segment is located downstream from the promoter, and transcription of the polynucleotide segment initiates at the promoter.

Various methods are used to administer the therapeutic composition directly to a specific site in the body. For example, a small metastatic lesion is located and the therapeutic composition injected several times in several different locations within the body of tumor. Alternatively, arteries which serve a tumor are identified, and the therapeutic composition injected into such an artery, in order to deliver the composition directly into the tumor. A tumor that has a necrotic center is aspirated and the composition injected directly into the now empty center of the tumor. The antisense composition is directly administered to the surface of the tumor, for example, by topical application of the composition. X-ray imaging is used to assist in certain of the above delivery methods.

Receptor-mediated targeted delivery of therapeutic compositions containing an antisense polynucleotide, subgenomic polynucleotides, or antibodies to specific tissues is also used. Receptor-mediated DNA delivery techniques are described in, for example, Findeis et al., Trends in Biotechnol. (1993) 11:202-205; Chiou et al., (1994) Gene Therapeutics: Methods And Applications Of Direct Gene Transfer (J. A. Wolff, ed.); Wu & Wu, J. Biol. Chem. (1988) 263:621-24; Wu et al., J. Biol. Chem. (1994) 269:542-46; Zenke et al., Proc. Natl. Acad. Sci. ( USA ) (1990) 87:3655-59; Wu et al., J. Biol. Chem. (1991) 266:338-42. Preferably, receptor-mediated targeted delivery of therapeutic compositions containing antibodies of the invention is used to deliver the antibodies to specific tissue.

Therapeutic compositions containing antisense subgenomic polynucleotides are administered in a range of about 100 ng to about 200 mg of DNA for local administration in a gene therapy protocol. Concentration ranges of about 500 ng to about 50 mg, about 1 mg to about 2 mg, about 5 mg to about 500 mg, and about 20 mg to about 100 mg of DNA can also be used during a gene therapy protocol. Factors such as method of action and efficacy of transformation and expression are considerations which will affect the dosage required for ultimate efficacy of the antisense subgenomic nucleic acids. Where greater expression is desired over a larger area of tissue, larger amounts of antisense subgenomic nucleic acids or the same amounts readministered in a successive protocol of administrations, or several administrations to different adjacent or close tissue portions of, for example, a tumor site, may be required to effect a positive therapeutic outcome. In all cases, routine experimentation in clinical trials will determine specific ranges for optimal therapeutic effect. A more complete description of gene therapy vectors, especially retroviral vectors, is contained in U.S. Ser. No. 08/869,309, which is expressly incorporated herein, and in section F below.

For genes encoding polypeptides or proteins with anti-inflammatory activity, suitable use, doses, and administration are described in U.S. Pat. No. 5,654,173, incorporated herein by reference. Therapeutic agents also include antibodies to proteins and polypeptides encoded by the subject nucleic acids, as described in U.S. Pat. No. 5,654,173.

F. Gene Therapy

The therapeutic nucleic acids of the present invention may be utilized in gene delivery vehicles. The gene delivery vehicle may be of viral or non-viral origin (see generally, Jolly, Cancer Gene Therapy (1994) 1:51-64; Kimura, Human Gene Therpay (1994) 5:845-852; Connelly, Human Gene Therapy (1995) 1:185-193; and Kaplitt, Nature Genetics (1994) 6:148-153). Gene therapy vehicles for delivery of constructs including a coding sequence of a therapeutic of the invention can be administered either locally or systemically. These constructs can utilize viral or non-viral vector approaches. Expression of such coding sequences can be induced using endogenous mammalian or heterologous promoters. Expression of the coding sequence can be either constitutive or regulated.

The present invention can employ recombinant retroviruses which are constructed to carry or express a selected nucleic acid molecule of interest. Retrovirus vectors that can be employed include those described in EP 0 415 731; WO 90/07936; WO 94/03622; WO 93/25698; WO 93/25234; U.S. Pat. No. 5, 219,740; WO 93/11230; WO 93/10218; Vile and Hart, Cancer Res. (1993) 53:3860-3864; Vile and Hart, Cancer Res. (1993) 53:962-967; Ram et al., Cancer Res. (1993) 53:83-88; Takamiya et al., J. Neurosci. Res. (1992) 33:493-503; Baba et al., J. Neurosurg. (1993) 79:729-735; U.S. Pat. no. 4,777,127; GB Patent No. 2,200,651; and EP 0 345 242. Preferred recombinant retroviruses include those described in WO 91/02805.

Packaging cell lines suitable for use with the above-described retroviral vector constructs may be readily prepared (see PCT publications WO 95/30763 and WO 92/05266), and used to create producer cell lines (also termed vector cell lines) for the production of recombinant vector particles. Within particularly preferred embodiments of the invention, packaging cell lines are made from human (such as HT1080 cells) or mink parent cell lines, thereby allowing production of recombinant retroviruses that can survive inactivation in human serum.

The present invention also employs alphavirus-based vectors that can function as gene delivery vehicles. Such vectors can be constructed from a wide variety of alphaviruses, including, for example, Sindbis virus vectors, Semliki forest virus (ATCC VR-67; ATCC VR-1247), Ross River virus (ATCC VR-373; ATCC VR-1246) and Venezuelan equine encephalitis virus (ATCC VR-923; ATCC VR-1250; ATCC VR 1249; ATCC VR-532). Representative examples of such vector systems include those described in U.S. Pat. Nos. 5,091,309; 5,217,879; and 5,185,440; and PCT Publication Nos. WO 92/10578; WO 94/21792; WO 95/27069; WO 95/27044; and WO 95/07994.

Gene delivery vehicles of the present invention can also employ parvovirus such as adeno-associated virus (AAV) vectors. Representative examples include the AAV vectors disclosed by Srivastava in WO 93/09239, Samulski et al., J. Vir. (1989) 63:3822-3828; Mendelson et al., Virol. (1988) 166:154-165; and Flotte et al., PNAS (1993) 90:10613-10617.

Representative examples of adenoviral vectors include those described by Berkner, Biotechniques (1988) 6:616-627; Rosenfeld et al., Science (1991) 252:431-434; WO 93/19191; Kolls et al., PNAS (1994) 91:215-219; Kass-Eisler et al., PNAS (1993) 90:11498-11502; Guzman et al., Circulation (1993) 88:2838-2848; Guzman et al., Cir. Res. (1993) 73:1202-1207; Zabner et al., Cell (1993) 75:207-216; Li et al., Hum. Gene Ther. (1993) 4:403-409; Cailaud et al., Eur. J. Neurosci. (1993) 5:1287-1291; Vincent et al., Nat. Genet. (1993) 5:130-134; Jaffe et al., Nat. Genet. (1992) 1:372-378; and Levrero et al., Gene (1991) 101:195-202. Exemplary adenoviral gene therapy vectors employable in this invention also include those described in WO 94/12649, WO 93/03769; WO 93/19191; WO 94/28938; WO 95/11984 and WO 95/00655. Administration of DNA linked to killed adenovirus as described in Curiel, Hum. Gene Ther. (1992) 3:147-154 may be employed.

Other gene delivery vehicles and methods may be employed, including polycationic condensed DNA linked or unlinked to killed adenovirus alone, for example Curiel, Hum. Gene Ther. (1992) 3:147-154; ligand linked DNA, for example see Wu, J. Biol. Chem. (1989) 264:16985-16987; eukaryotic cell delivery vehicles cells, for example see U.S. Ser. No. 08/240,030, filed May 9, 1994, and U.S. Ser. No. 08/404,796; deposition of photopolymerized hydrogel materials; hand-held gene transfer particle gun, as described in U.S. Pat. No. 5,149,655; ionizing radiation as described in U.S. Pat. No. 5,206,152 and in WO92/11033; nucleic charge neutralization or fusion with cell membranes. Additional approaches are described in Philip, Mol. Cell Biol. (1994)14:2411-2418, and in Woffendin, Proc. Natl. Acad. Sci. (1994) 91:1581-1585.

Naked DNA may also be employed. Exemplary naked DNA introduction methods are described in WO 90/11092 and U.S. Pat. No. 5,580,859. Uptake efficiency may be improved using biodegradable latex beads. DNA coated latex beads are efficiently transported into cells after endocytosis initiation by the beads. The method may be improved further by treatment of the beads to increase hydrophobicity and thereby facilitate disruption of the endosome and release of the DNA into the cytoplasm. Liposomes that can act as gene delivery vehicles are described in U.S. Pat. No. 5,422,120, PCT Nos. WO 95/13796, WO 94/23697, and WO 91/14445, and EP No. 0 524 968.

Further non-viral delivery suitable for use includes mechanical delivery systems such as the approach described in Woffendin et al., Proc. Natl. Acad. Sci. USA (1994) 91(24):11581-11585. Moreover, the coding sequence and the product of expression of such can be delivered through deposition of photopolymerized hydrogel materials. Other conventional methods for gene delivery that can be used for delivery of the coding sequence include, for example, use of hand-held gene transfer particle gun, as described in U.S. Pat. No. 5,149,655; use of ionizing radiation for activating transferred gene, as described in U.S. Pat. No. 5,206,152 and PCT No. WO 92/11033.

G. Transoenic Animals

One aspect of the present invention relates to transgenic non-human animals having germline and/or somatic cells in which the biological activity of one or more genes are altered by a chromosomally incorporated transgene.

In a preferred embodiments, the transgene encodes a mutant protein, such as dominant negative protein which antagonizes at least a portion of the biological function of a wild-type protein.

Yet another preferred transgenic animal includes a transgene encoding an antisense transcript which, when transcribed from the transgene, hybridizes with a gene or a mRNA transcript thereof, and inhibits expression of the gene.

In one embodiment, the present invention provides a desired non-human animal or an animal (including human) cell which contains a predefined, specific and desired alteration rendering the non-human animal or animal cell predisposed to cancer. Specifically, the invention pertains to a genetically altered non-human animal (most preferably, a mouse), or a cell (either non-human animal or human) in culture, that is defective in at least one of two alleles of a tumor-suppressor gene. The inactivation of at least one of these tumor suppressor alleles results in an animal with a higher susceptibility to tumor induction or other proliferative or differentiative disorders, or disorders marked by aberrant signal transduction, e.g., from a cytokine or growth factor. A genetically altered mouse of this type is able to serve as a useful model for hereditary cancers and as a test animal for carcinogen studies. The invention additionally pertains to the use of such non-human animals or animal cells, and their progeny in research and medicine.

Furthermore, it is contemplated that cells of the transgenic animals of the present invention can include other transgenes, e.g., which alter the biological activity of a second tumor suppressor gene or an oncogene. For instance, the second transgene can functionally disrupt the biological activity of a second tumor suppressor gene, such as p53, p73, DCC, p21 cip1 , p27 kip1 , Rb, Mad or E2F. Alternatively, the second transgene can cause overexpression or loss of regulation of an oncogene, such as ras, myc, a cdc25 phosphatase, Bc1-2, Bc1-6, a transforming growth factor, neu, int-3, polyoma virus middle T antigen, SV40 large T antigen, a papillomaviral E6 protein, a papillomaviral E7 protein, CDK4, or cyclin D1.

A preferred transgenic non-human animal of the present invention has germline and/or somatic cells in which one or more alleles of a gene are disrupted by a chromosomally incorporated transgene, wherein the transgene includes a marker sequence providing a detectable signal for identifying the presence of the transgene in cells of the transgenic animal, and replaces at least a portion of the gene or is inserted into the gene or disrupts expression of a wild-type protein.

Still another aspect of the present invention relates to methods for generating non-human animals and stem cells having a functionally disrupted endogenous gene. In a preferred embodiment, the method comprises the steps of:

(i) constructing a transgene construct including (a) a recombination region having at least a portion of the gene, which recombination region directs recombination of the transgene with the gene, and (b) a marker sequence which provides a detectable signal for identifying the presence of the transgene in a cell;

(ii) transfering the transgene into stem cells of a non-human animal;

(iii) selecting stem cells having a correctly targeted homologous recombination between the transgene and the gene;

(iv) transfering cells identified in step (iii) into a non-human blastocyst and implanting the resulting chimeric blastocyst into a non-human female; and

(v) collecting offspring harboring an endogenous gene allele having the correctly targeted recombination.

Yet another aspect of the invention provides a method for evaluating the carcinogenic potential of an agent by (i) contacting a transgenic animal of the present invention with a test agent, and (ii) comparing the number of transformed cells in a sample from the treated animal with the number of transformed cells in a sample from an untreated transgenic animal or transgenic animal treated with a control agent. The difference in the number of transformed cells in the treated animal, relative to the number of transformed cells in the absence of treatment with a control agent, indicates the carcinogenic potential of the test compound.

Another aspect of the invention provides a method of evaluating an anti-proliferative activity of a test compound. In preferred embodiments, the method includes contacting a transgenic animal of the present invention, or a sample of cells from such animal, with a test agent, and determining the number of transformed cells in a specimen from the transgenic animal or in the sample of cells. A statistically significant decrease in the number of transformed cells, relative to the number of transformed cells in the absence of the test agent, indicates the test compound is a potential anti-proliferative agent.

The practice of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, transgenic biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example, Molecular Cloning A Laboratory Manual, 2nd Ed., ed. by Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory Press:1989); DNA Cloning , Volumes I and II (D. N. Glover ed., 1985); Oligonucleotide Synthesis (M. J. Gait ed., 1984); Mullis et al. U.S. Pat. No. 4,683,195 ; Nucleic Acid Hybridization (B. D. Hames & S. J. Higgins eds. 1984); Transcription And Translation (B. D. Hames & S. J. Higgins eds. 1984); Culture Of Animal Cells (R. I. Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells And Enzymes (IRL Press, 1986); B. Perbal, A Practical Guide To Molecular Cloning (1984); the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); Gene Transfer Vectors For Mammalian Cells (J. H. Miller and M. P. Calos eds., 1987, Cold Spring Harbor Laboratory); Methods In Enzymology , Vols. 154 and 155 (Wu et al. eds.), Immunochemical Methods In Cell And Molecular Biology (Mayer and Walker, eds., Academic Press, London, 1987); Handbook Of Experimental Immunology , Volumes I-IV (D. M. Weir and C. C. Blackwell, eds., 1986); Manipulating the Mouse Embryo , (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).

As mentioned above, the sequences described herein are believed to have particular utility in regards to colon cancer. However, they may also be useful with other types of cancers and other disease states.

The present invention will now be illustrated by reference to the following examples which set forth particularly advantageous embodiments. However, it should be noted that these embodiments are illustrative and are not to be construed as restricting the invention in any way.

XI. EXAMPLES

A. Identification of Differentially Expressed Sequences

Description of the Libraries

SEQ ID Nos: 1-544 were derived from libraries designated as DE and PA as described below. The DE library is a normalized, colon cancer specific, subtracted cDNA library. The DE library is specific for sequences expressed in colon cancer [proximal and distal Dukes' B, microsatellite instability negative (MSI−)] but not expressed in normal tissues, including normal colon tissue. The PA library is a normalized, colon specific, subtracted cDNA library. The PA library is specific for sequences expressed in normal colon tissue but not expressed in other normal tissues.

Construction of a Colon Cancer Specific Library

A subtracted colon cancer specific library was made by subtracting pooled proximal, stage B, MSI − and distal, Stage B, MSI − tumor tissue cDNA against a combination of pooled driver normal cDNA made from colon, peripheral blood leukocytes (PBL), liver, spleen, lung, kidney, heart, small intestine, skeletal muscle, and prostate tissue cDNAs. The following RNA samples were obtained from Origene Technologies, Inc., Rockville, Md., and were used to synthesize the pooled driver cDNA: #HT-1015 normal colon total RNA, #HT-1005 liver total RNA, #HT-1004 spleen total RNA, #HT-1009 lung total RNA, #HT-1003 kidney total RNA, #HT-1006 peripheral blood leukocyte total RNA, #HT-prostate total RNA, #HM-1002 heart muscle poly A+RNA, #HM-1007 intestine poly A+RNA, and #HM-1008 skeletal muscle poly A+RNA. First-strand cDNA was prepared for each using 1 microgram of RNA. A biased pool of first-strand cDNA was prepared containing 50% normal colon first-strand cDNA reaction and 5.56% of each of the remaining tissue first-strand cDNA reactions by volume. Eight individual amplification reactions, each containing 1 microliter of the biased first-strand cDNA reaction pool, were performed for 18 cycles. The double stranded cDNA product from all eight amplification reactions were pooled and purified for subsequent use in subtractive hybridization. The colon cancer specific subtracted library was called DE and individual clones derived from this library were referred to with a number prefixed by DE.

Normalized subtracted DE colon cancer specific and pooled normal human tissue specific cDNA libraries (same as components of driver cDNA above) were generated according published procedures (Daitchenko et al., 1996 PNAS 93:6025-6030, Gurskaya et al., 1996 Analytical Biochemistry 240:90-97) using Clontech Laboratories, Inc., PCR-Select cDNA subtraction kit, PT1117-1. A forty-five fold mass excess of driver cDNA (450 nanograms) was used for each subtraction experiment. Subtractive hybridization of tester with driver cDNAs was performed twice, each time for about 8-12 hours. Subtracted cancer specific DE cDNA was ligated into the pCR2.1-TOPO plasmid vector (Invitrogen Corporation, Carlsbad Calif.) and chemically transformed into ultracompetent Epicurian E. coli XL10-Gold cells (Stratagene, La Jolla, Calif.). A reverse library was also constructed wherein the tester and driver samples were switched; this library was designated as MD.

Construction of a Normal Colon Specific Library

This normal colon tissue specific library was made using Clontech Laboratories Inc PCR-Select kit, K1804-1, following instructions from the users manual (PT1117-1).

Four, 100 μl, SMART PCR cDNA amplification reactions for each normal, non-cancerous, patient sample, were performed, starting with 1 μl from their respective first strand cDNA reactions. Each sample was amplified for only 18 cycles using the following PCR conditions; 95 C-10 sec, 68 C 5 min. using a 9600 Perkin Elmer instrument. The following are Bayer Diagnostic sample identification numbers for the cDNA samples that were amplified: NPB(−) 27347, NPB(−)27859, NPB(−)28147, NPB(−)28162, NDB(−)28800, NDB(−)29243, NDB(−)29244 and NDB(−)42472. These are normal colon tissue samples obtained from the same patients providing the proximal stage B MSI− and distal stage B MSI− cancer samples, which were used to prepare the DE library described above. Equal volumes of the eight normal colon cDNAs were pooled. A subtracted normal colon tissue specific library was made by subtracting the normal colon cDNA pool against a combination of pooled driver normal cDNA made from peripheral blood leukocytes (PBL), liver, spleen, lung, kidney, heart, small intestine, skeletal muscle, and prostate tissue cDNAs. The following are the RNA samples that were used to synthesize the pooled driver cDNA: #HT-1005 liver total RNA, #HT-1004 spleen total RNA, #HT-1009 lung total RNA, #HT-1003 kidney total RNA, #HT-1006 peripheral blood leukocyte total RNA, #HT-prostate total RNA, #HM-1002 heart muscle poly A+RNA, #HM-1007 intestine poly A+RNA, and #HM-1008 skeletal muscle poly A+RNA. First-strand cDNA was prepared for each using 1 microgram of RNA. A pool of first strand cDNA reactions was then made consisting of equal volumes of the nine driver tissue first-strand cDNA reactions. Eight individual amplification reactions, each containing 1 microliter of the first-strand cDNA reaction pool, were performed for 18 cycles. The double stranded cDNA product from all eight amplification reactions was pooled and purified for subsequent use in subtractive hybridization. The normal colon tissue specific subtracted library was called PA and individual clones derived from this library were referred to with a number prefixed by PA.

The normalized subtracted PA normal colon specific cDNA library and a subtracted normal human tissue specific cDNA library, consisting of the human tissues listed above were generated according published procedures (Daitchenko et al., 1996 PNAS 93:6025-6030, Gurskaya et al., 1996 Analytical Biochemistry 240:90-97) using Clontech Laboratories, Inc., PCR-Select cDNA subtraction kit, PTI 117-1. Library construction and cloning were carried out as described above for the colon cancer specific library. Out of the 1152 clones that were analyzed for differential expression, approximately 69% were differentially expressed.

Each EST isolated from each of the above libraries represents a sequence from a partial mRNA transcript, since the cDNA used for making the subtracted library was restricted with RsaI, a four base cutter restriction endonuclease that generates fragments with an average size of about 600 base pairs.

Validation of Differential Expression in Colon Cancer

To validate that the differentially expressed sequences found in this library were specific to colon cancer, the clones were screened with cDNAs prepared from a colon cancer specific library, Delaware (DE), and a normal tissue specific library Maryland (MD). cDNA clones were analyzed for differential expression following the procedure developed by von Stein et al., 1997, Nucleic Acids Research 25(13):2598-2602 and using probes synthesized according to a published method (Jin et al., 1997, Biotechniques 23:1083-1086). Out of the 1248 clones that were analyzed for differential expression approximately 83% were differentially expressed.

Sequencing and Analysis of Differentially Expressed Clones

The nucleotide sequence of the inserts from clones shown to be differentially expressed was determined by single-pass sequencing from either the T7 or M13 promoter sites using fluorescently labeled dideoxynucleotides via the Sanger sequencing method. Sequences were analyzed according to methods described in the text (XI., Examples; B. Results of Public Database Search).

Each nucleic acid represents sequence from at least a partial mRNA transcript. The nucleic acids of the invention were assigned a sequence identification number (see attachments). The nucleic acid sequences are provided in the attached Sequence Listing.

An example of an experiment to identify differentially expressed clones is shown in the FIGURE, “Differential Expression Analysis”. The inserts from subtracted clones were amplified, electrophoresed, and blotted on to membranes as described above. The gel was hybridized with RSAI cut DE and MD cDNA probes as described above.

In the FIGURE, individual clones are designated by a number at the top of each lane; the blots are aligned so that the same clone is represented in the same vertical lane in both the upper (“Cancer Probe”) and lower (“Normal Probe”) blot. Lanes labeled “O” indicate clones that are overexpressed, i.e., show a darker, more prominent band in the upper blot (“Cancer Probe”) relative to that observed, in the same lane, in the lower blot (“Normal Probe”). The Lane labeled “U” indicates a clone that is underexpressed, i.e., shows a darker, more prominent band in the lower blot (“Normal Probe”) relative to that observed, in the same lane, in the upper blot (“Cancer Probe”). The lane labeled “M”, indicates a clone that is marginally overexpressed in cancer and normal cells.

B. Results of Public Databases Searches

The nucleotide sequence of SEQ ID Nos. 1-544 were aligned with individual sequences that were publicly available. Genbank and divisions of GenBank, such as dbEST, CGAP, and Unigene were the primary databases used to perform the sequence similarity searches. The patent database, GENESEQ, was also utilized.

A total of 544 sequences were analyzed. The sequences were first masked to identify vector-derived sequences, which were subsequently removed. The remaining sequence information was used to create the Sequence Listing (SEQ ID Nos. 1-544). Each of these sequences was used as the query sequence to perform a Blast 2 search against the databases listed above. The Blast 2 search differs from the traditional Blast search in that it allows for the introduction of gaps in order to produce an optimal aligrunent of two sequences.

A proprietary algorithm was developed to utilize the output from the Blast 2 searches and categorize the sequences based upon high similarity (e value<1e-40) or identity to entries contained in the GenBank and dbEST databases. Three categories were created as follows: 1) matches to known human genes, 2) matches to human EST sequences, and 3) no significant match to either 1 or 2, and therefore a potentially novel human sequence.

Those skilled in the art will recognize, or be able to ascertain, using not more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such specific embodiments and equivalents are intended to be encompassed by the following claims.

All patents, published patent applications, and publications cited herein are incorporated by reference as if set forth fully herein.

TABLE 1
SEQ		Tissue	SEQ		Tissue
ID NO	clone name	Probe	ID NO	clone name	Probe
1	de0020t7	U	273	de0214t7	ND
2	de0041t7	N	274	de0215t7	ND
3	de0056t7	U	275	de0218t7	ND
4	de0064t7	N	276	de0221t7	ND
5	de0092t7	U	277	de0223t7	O
6	de0142t7	N	278	de0227t7	ND
7	de0153t7	M	279	de0229t7	O
8	de0163t7	U	280	de0230t7	ND
9	de0188t7	N	281	de0232t7	ND
10	de0190t7	U	282	de0234t7	ND
11	de0201t7	M	283	de0235t7	ND
12	de0225t7	U	284	de0237t7	ND
13	de0246t7	U	285	de0238t7	ND
14	de0257t7	N	286	de0239t7	N
15	de0285t7	O	287	de0241t7	N
16	de0529t7	U	288	de0242t7	O
17	de0629t7	U	289	de0244t7	N
18	de0727t7	O	290	de0247t7	O
19	de0787t7	U	291	de0252t7	ND
20	de0810t7	N	292	de0255t7	N
21	de0833t7	N	293	de0256t7	ND
22	pa0107t7	U	294	de0260t7	N
23	pa0130t7	U	295	de0261t7	N
24	pa0149t7	U	296	de0263t7	N
25	pa0185t7	U	297	de0264t7	ND
26	pa0203t7	U	298	de0265t7	ND
27	pa0277t7	U	299	de0266t7	O
28	pa0287t7	U	300	de0267t7	N
29	pa0293t7*	U	301	de0268t7	ND
30	pa0341t7	U	302	de0272t7	ND
31	pa0357t7	N	303	de0273t7	ND
32	pa0361t7	U	304	de0274t7	N
33	pa0404t7	U	305	de0276t7	O
34	pa0408t7	U	306	de0277t7	M
35	pa0425t7	N	307	de0279t7	N
36	de0001t7	N	308	de0280t7	ND
37	de0002t7	N	309	de0281t7	N
38	de0036t7	N	310	de0282t7	ND
39	de0038t7	M	311	de0284t7	ND
40	de0040t7	N	312	de0286t7	ND
41	de0043t7	O	313	de0339t7	ND
42	de0044t7	N	314	de0483t7	ND
43	de0045t7	N	315	de0484t7	M
44	de0050t7	N	316	de0491t7	ND
45	de0052t7	N	317	de0499t7	ND
46	de0054t7	N	318	de0507t7	M
47	de0055t7	N	319	de0511t7	O
48	de0059t7	O	320	de0519t7	ND
49	de0060t7	N	321	de0520t7	N
50	de0063t7	U	322	de0522t7	ND
51	de0066t7	O	323	de0524t7	M
52	de0067t7	O	324	de0530t7	ND
53	de0079t7	N	325	de0531t7	ND
54	de0085t7	N	326	de0532t7	M
55	de0089t7	N	327	de0534t7	N
56	de0095t7	N	328	de0542t7	ND
57	de0099t7	N	329	de0556t7	M
58	de0105t7	N	330	de0557t7	ND
59	de0112t7	N	331	de0559t7	U
60	de0114t7	N	332	de0562t7	ND
61	de0121t7	N	333	de0566t7	U
62	de0122t7	N	334	de0567t7	N
63	de0124t7	N	335	de0568t7	ND
64	de0139t7	M	336	de0570t7	ND
65	de0143t7	N	337	de0571t7	ND
66	de0166t7	U	338	de0574t7	ND
67	de0168t7	N	339	de0581t7	ND
68	de0171t7	N	340	de0583t7	U
69	de0178t7	N	341	de0587t7	ND
70	de0180t7	O	342	de0588t7	ND
71	de0181t7	N	343	de0591t7	ND
72	de0199t7	N	344	de0592t7	ND
73	de0200t7	N	345	de0597t7	U
74	de0202t7	N	346	de0598t7	ND
75	de0205t7	N	347	de0599t7	ND
76	de0207t7	U	348	de0602t7	N
77	de0212t7	N	349	de0605t7	ND
78	de0217t7	N	350	de0608t7	ND
79	de0220t7	U	351	de0610t7	ND
80	de0228t7	N	352	de0616t7	O
81	de0236t7	O	353	de0619t7	U
82	de0243t7	N	354	de0620t7	ND
83	de0253t7	O	355	de0622t7	ND
84	de0258t7	N	356	de0623t7	ND
85	de0259t7	N	357	de0624t7	O
86	de0262t7	N	358	de0625t7	ND
87	de0270t7	N	359	de0628t7	ND
88	de0275t7	N	360	de0630t7	ND
89	de0287t7	N	361	de0631t7	ND
90	de0288t7	N	362	de0632t7	N
91	de0306t7	N	363	de0634t7	ND
92	de0490t7	N	364	de0639t7	ND
93	de0501t7	M	365	de0642t7	ND
94	de0516t7	N	366	de0649t7	ND
95	de0589t7	N	367	de0650t7	N
96	de0596t7	U	368	de0656t7	N
97	de0600t7	N	369	de0657t7	ND
98	de0609t7	U	370	de0660t7	ND
99	de0611t7	N	371	de0661t7	O
100	de0617t7	U	372	de0662t7	O
101	de0633t7	N	373	de0664t7	ND
102	de0643t7	N	374	de0665t7	ND
103	de0647t7	M	375	de0667t7	ND
104	de0652t7	N	376	de0669t7	ND
105	de0666t7	N	377	de0676t7	ND
106	de0695t7	U	378	de0686t7	N
107	de0705t7	N	379	de0687t7	ND
108	de0706t7	M	380	de0689t7	N
109	de0708t7	N	381	de0691t7	M
110	de0724t7	N	382	de0693t7	ND
111	de0735t7	N	383	de0703t7	ND
112	de0740t7	N	384	de0704t7	M
113	de0742t7	N	385	de0707t7	O
114	de0747t7	N	386	de0709t7	O
115	de0764t7	N	387	de0710t7	ND
116	de0777t7	O	388	de0712t7	N
117	de0781t7	N	389	de0715t7	ND
118	de0793t7	U	390	de0719t7	N
119	de0794t7	N	391	de0722t7	ND
120	de0798t7	N	392	de0723t7	ND
121	de0800t7	O	393	de0725t7	N
122	de0816t7	N	394	de0728t7	ND
123	de0818t7	N	395	de0729t7	ND
124	de0835t7	N	396	de0731t7	ND
125	pa0078t7	U	397	de0732t7	ND
126	pa0080t7	N	398	de0737t7	ND
127	pa0088t7	U	399	de0739t7	M
128	pa0089t7	U	400	de0741t7	ND
129	pa0095t7	U	401	de0744t7	N
130	pa0158t7	U	402	de0746t7	ND
131	pa0159t7	U	403	de0749t7	N
132	pa0187t7	N	404	de0750t7	ND
133	pa0190t7	U	405	de0756t7	ND
134	pa0192t7	U	406	de0759t7	ND
135	pa0209t7	U	407	de0761t7	O
136	pa0215t7	N	408	de0762t7	ND
137	pa0218t7	N	409	de0766t7	ND
138	pa0220t7	N	410	de0768t7	U
139	pa0238t7	N	411	de0769t7	ND
140	pa0249t7	U	412	de0772t7	ND
141	pa0256t7	N	413	de0776t7	ND
142	pa0258t7	U	414	de0779t7	ND
143	pa0272t7	N	415	de0785t7	ND
144	pa0283t7	N	416	de0786t7	ND
145	pa0295t7	N	417	de0788t7	ND
146	pa0309t7	U	418	de0789t7	ND
147	pa0314t7	N	419	de0792t7	ND
148	pa0317t7	N	420	de0796t7	ND
149	pa0319t7	N	421	de0797t7	ND
150	pa0323t7	N	422	de0801t7	O
151	pa0333t7	N	423	de0804t7	ND
152	pa0336t7	N	424	de0805t7	ND
153	pa0353t7	N	425	de0806t7	ND
154	pa0363t7	N	426	de0807t7	N
155	pa0364t7	N	427	de0811t7	O
156	pa0366t7	U	428	de0812t7	ND
157	pa0382t7	N	429	de0817t7	N
158	pa0383t7	N	430	de0820t7	ND
159	pa0388t7	N	431	de0821t7	ND
160	pa0389t7	N	432	de0822t7	ND
161	pa0405t7	N	433	de0823t7	N
162	pa0406t7	N	434	de0824t7	N
163	pa0409t7	U	435	de0825t7	ND
164	pa0411t7	N	436	de0826t7	ND
165	pa0417t7	N	437	de0827t7	ND
166	pa0421t7	U	438	de0829t7	ND
167	pa0429t7	U	439	de0830t7	ND
168	pa0432t7	U	440	de0837t7	N
169	de0004t7	U	441	de0840t7	ND
170	de0008t7	ND	442	de0848t7	ND
171	de0009t7	ND	443	pa0079t7	N
172	de0010t7	ND	444	pa0081t7	ND
173	de0011t7	ND	445	pa0082t7	ND
174	de0012t7	ND	448	pa0083t7	ND
175	de0013t7	ND	447	pa0084t7	ND
176	de0014t7	ND	448	pa0085t7	ND
177	de0016t7	ND	449	pa0086t7	M
178	de0017t7	ND	450	pa0090t7	N
179	de0018t7	M	451	pa0091t7	ND
180	de0019t7	ND	452	pa0092t7	N
181	de0023t7	O	453	pa0096t7	ND
182	de0024t7	N	454	pa0100t7	ND
183	de0029t7	ND	455	pa0101t7	U
184	de0030t7	ND	456	pa0103t7	ND
185	de0032t7	ND	457	pa0104t7	ND
186	de0033t7	O	458	pa0114t7	ND
187	de0034t7	ND	459	pa0115t7	ND
188	de0035t7	ND	460	pa0118t7	ND
189	de0042t7	ND	461	pa0120t7	ND
190	de0047t7	ND	462	pa0129t7	ND
191	de0048t7	N	463	pa0131t7	U
192	de0049t7	ND	464	pa0133t7	ND
193	de0051t7	O	465	pa0135t7	N
194	de0053t7	ND	466	pa0140t7	O
195	de0065t7	ND	467	pa0142t7	ND
196	de0068t7	N	468	pa0143t7	ND
197	de0069t7	ND	469	pa0146t7	ND
198	de0071t7	N	470	pa0147t7	ND
199	de0072t7	ND	471	pa0148t7	ND
200	de0076t7	U	472	pa0151t7	ND
201	de0077t7	ND	473	pa0157t7	ND
202	de0078t7	ND	474	pa0164t7	ND
203	de0080t7	ND	475	pa0167t7	N
204	de0082t7	ND	476	pa0171t7	U
205	de0086t7	ND	477	pa0174t7	ND
206	de0087t7	ND	478	pa0175t7	ND
207	de0088t7	ND	479	pa0179t7	N
208	de0093t7	N	480	pa0182t7	ND
209	de0094t7	ND	481	pa0184t7	ND
210	de0097t7	O	482	pa0186t7	U
211	de0098t7	ND	483	pa0189t7	ND
212	de0100t7	ND	484	pa0207t7	ND
213	de0101t7	ND	485	pa0210t7	ND
214	de0102t7	ND	486	pa0212t7	ND
215	de0106t7	ND	487	pa0214t7	ND
216	de0109t7	U	488	pa0216t7	ND
217	de0110t7	N	489	pa0217t7	M
218	de0111t7	N	490	pa0219t7	N
219	de0113t7	ND	491	pa0223t7	ND
220	de0115t7	O	492	pa0224t7	ND
221	de0117t7	ND	493	pa0228t7	ND
222	de0118t7	U	494	pa0229t7	U
223	de0119t7	ND	495	pa0231t7	ND
224	de0123t7	ND	496	pa0232t7	ND
225	de0125t7	ND	497	pa0240t7	ND
226	de0126t7	ND	498	pa0252t7	ND
227	de0129t7	ND	499	pa0260t7	U
228	de0130t7	U	500	pa0261t7	N
229	de0131t7	O	501	pa0262t7	ND
230	de0132t7	ND	502	pa0264t7	N
231	de0134t7	O	503	pa0265t7	N
232	de0135t7	ND	504	pa0268t7	ND
233	de0137t7	M	505	pa0276t7	ND
234	de0138t7	ND	506	pa0279t7	ND
235	de0140t7	ND	507	pa0280t7	ND
236	de0141t7	ND	508	pa0282t7	ND
237	de0145t7	ND	509	pa0285t7	ND
238	de0146t7	O	510	pa0299t7	ND
239	de0148t7	ND	511	pa0300t7	U
240	de0149t7	ND	512	pa0301t7	ND
241	de0151t7	O	513	pa0302t7	ND
242	de0152t7	ND	514	pa0305t7	N
243	de0154t7	ND	515	pa0306t7	ND
244	de0156t7	ND	516	pa0307t7	ND
245	de0157t7	U	517	pa0311t7	ND
246	de0158t7	ND	518	pa0316t7	ND
247	de0159t7	N	519	pa0318t7	ND
248	de0162t7	ND	520	pa0321t7	M
249	de0169t7	U	521	pa0325t7	N
250	de0170t7	O	522	pa0326t7	ND
251	de0174t7	ND	523	pa0332t7	ND
252	de0176t7	ND	524	pa0339t7	ND
253	de0177t7	O	525	pa0346t7	O
254	de0182t7	ND	526	pa0349t7	ND
255	de0183t7	ND	527	pa0351t7	U
256	de0184t7	ND	528	pa0355t7	ND
257	de0186t7	ND	529	pa0358t7	ND
258	de0187t7	M	530	pa0360t7	N
259	de0189t7	ND	531	pa0362t7	ND
260	de0191t7	M	532	pa0368t7	U
261	de0192t7	ND	533	pa0369t7	ND
262	de0193t7	ND	534	pa0373t7	ND
263	de0195t7	N	535	pa0380t7	ND
264	de0196t7	N	536	pa0393t7	ND
265	de0197t7	N	537	pa0395t7	ND
266	de0198t7	ND	538	pa0396t7	ND
267	de0203t7	ND	539	pa0397t7	ND
268	de0208t7	ND	540	pa0410t7	N
269	de0209t7	N	541	pa0415t7	ND
270	de0210t7	N	542	pa0416t7	ND
271	de0211t7	ND	543	pa0424t7	ND
272	de0213t7	ND	544	pa0430t7	ND
*In the provisional application (60/098,639) filed August 31, 1998, clone PA0293t7 was labeled clone PA0023t7 in error. That mistake has been corrected here to reflect the accurate clone name.

TABLE 2
		“Novel” Region 1	“Novel” Region 2
SEQ ID NO	Clone name	Start/Stop	Start/Stop	GenBank Identifier for top 5 matching EST sequences
36.00	de0001t7	439-607		g835668	g857149	g1321047	g1968601	g1476832
40.00	de0040t7	1-201		g2166831	g4136486	g1747976	g1180529	g2265195
41.00	de0043t7	467-615		g5129477	g1801229	g1845053	g1544683	g1694347
43.00	de0045t7	1-228		g2322205	g1139955	g4267203	g2165927	g3039227
45.00	de0052t7	455-628		g1523492	g1548890	g1523465	g1809433	g5132985
50.00	de0063t7	1-114	452-624	g2197338	g5754794	g2694448	g2070840	g3419233
51.00	de0066t7	301-631		g2162184	g749398	g1239250	g839454	g1966148
52.00	de0067t7	391-623		g1521548	g848102	g1349419	g1196287	g771178
54.00	de0085t7	415-565		g1367045	g1367136	g2337716	g841637	g795336
63.00	de0124t7	411-605		g1809451	g1757444	g3181138	g2905518	g1157799
64.00	de0139t7	424-612		g3899105	g3431615	g3246439	g1312989	g1182375
65.00	de0143t7	479-598		g1239204	g1067288	g1080541	g4876470	g1188553
68.00	de0171t7	443-611		g867521	g1636718	g2162333	g2342197	g1466482
69.00	de0178t7	485-603		g1371240	g2055704	g2208007	g1686872	g1740908
71.00	de0181t7	1-153		g1188057	g1018287	g1447796	g1025264	g1069169
73.00	de0200t7	1-218	384-581	g1972267	g1989383	g964966	g2883986	g483738
74.00	de0202t7	448-599		g2115372	g1959491	g1329334	g1198642	g1957432
75.00	de0205t7	1 to 75		g779809	g2167738	g2537620	g2656428
77.00	de0212t7	1-185		g4265939	g1548503	g1687914	g1716864	g877386
80.00	de0228t7	411-594		g3446139	g3745043	g1126367	g2163321	g1195781
82.00	de0243t7	253-604		g2001999	g1071313	g966668	g26974
83.00	de0253t7	1-133		g2111781	g1663818	g574791	g1406232	g1663812
85.00	de0259t7	241-602		g2216159	g5177204	g1969363	g1388290	g1389464
86.00	de0262t7	351-583		g1025700	g2019225	g2080424	g1547366	g728148
88.00	de0275t7	455-592		g5665082	g5553136	g5552975	g389141	g1665092
89.00	de0287t7	364-630		g2026446	g4622337	g2021046	g2056125	g5037418
92.00	de0490t7	1-264	482-653	g1812285	g2816130	g2818085	g2819140	g1194260
96.00	de0596t7	362-655		g1155862	g1991972	g1996949	g1149020	g3307331
97.00	de0600t7	1 to 71		g883470	g1880085	g3162627	g3162628	g918039
98.00	de0609t7	434-582		g5037002	g1404408	g2816378	g759987	g2969638
102.00	de0643t7	433-605		g1382697	g5236495	g5235876	g5177792	g4453929
105.00	de0666t7	385-586		g2932996	g1010052	g2616680	g3277252	g2252166
106.00	de0695t7	401-644		g4897106	g1741190	g1501550	g3932286	g781364
107.00	de0705t7	431-599		g5176918	g1551249	g4739742	g2540062	g2583382
108.00	de0706t7	407-620		g826820	g870098	g1685556	g1687983	g685342
110.00	de0724t7	127-192	423-603	g2036407	g1969842	g616903	g1218717	g1745366
113.00	de0742t7	1 to 49		g2596046	g3277962	g4665052	g895453	g1521473
116.00	de0777t7	270-610		g2358992	g1692100	g1979572	g1720777	g1547963
117.00	de0781t7	457-608		g1982258	g1379170	g1496197	g1981084	g1982434
118.00	de0793t7	435-577		g1860678	g1991856	g946392	g4703537	g5741112
119.00	de0794t7	342-584		g4394682	g3418148	g3424422	g1398292	g4985474
120.00	de0798t7	1 to 65	384-585	g1570282	g4817443	g3891031	g2028723	g2357411
121.00	de0800t7	270-568		g2358992	g1892100	g1979572	g1720777	g1692006
122.00	de0816t7	485-550		g2014274	g2189652	g1087845	g2013302	g643722
123.00	de0818t7	387-573		g4190027	g4900502	g3601481	g4070577	g4112474
124.00	de0835t7	330-570		g2029304	g2029457	g1544689	g1947895	g2986865
129.00	pa0095t7	1-370		g5340876	g771049	g791906	g677786
132.00	pa0187t7	388-593		g2029457	g2029304	g1544689	g1947895	g2986865
134.00	pa0192t7	444-618		g4897608	g1815096	g2051120	g3426889	g4690585
139.00	pa0238t7	364-586		g4728995	g4971678	g1615267	g1501282	g5447095
140.00	pa0249t7	124-588		g2061363	g2060961	g2060863	g1135265	g2060372
142.00	pa0258t7	254-595		g2784639	g2276958	g968701	g1670101	g2329615
143.00	pa0272t7	1 to 98		g2198976	g1961065	g2188645	g1965162	g1852287
148.00	pa0317t7	457-612		g5113829	g2080750	g3739118	g3753615	g2933157
153.00	pa0353t7	357-620		g2029457	g2029304	g2986865	g1544689	g1947895
156.00	pa0366t7	354-760		g5339118	g775873	g610250	g3886660	g2052048
157.00	pa0382t7	395-668		g3886319	g968094	g2216376	g4126052	g1747689
159.00	pa0388t7	492-739		g1636721	g2006249	g5035641	g3430502	g778670
160.00	pa0389t7	1-177		g1321159	g1320039	g1371307	g2070781	g1350314
161.00	pa0405t7	119-214		g4824527	g4852801	g1368093	g1392201	g1350038
164.00	pa0411t7	289-345		g4810371	g2369264	g3163382	g3839554	g1950020
166.00	pa0421t7	233-745		g5747013	g4150749	g1482715	g1137706	g3900569

TABLE 3
The following list of clones indicates those found in either
the DE or PA libraries and the SW480 library
SEQ ID NO clone name
185       de0032t7
186       de0033t7
193       de0051t7
196       de0068t7
240       de0149t7
241       de0151t7
247       de0159t7
72        de0199t7
279       de0229t7
281       de0232t7
283       de0235t7
306       de0277t7
310       de0282t7
318       de0507t7
328       de0542t7
331       de0559t7
342       de0588t7
359       de0628t7
375       de0667t7
379       de0687t7
407       de0761t7
410       de0768t7
427       de0811t7
466       pa0140t7
470       pa0147t7
481       pa0184t7
493       pa0228t7
494       pa0229t7
140       pa0249t7
506       pa0279t7
510       pa0299t7
515       pa0306t7
517       pa0311t7
518       pa0316t7
536       pa0393t7
539       pa0397t7
544       pa0430t7

544 1 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 1gcgtggtcng gccgaggtac agtctcggct cactgcaatc tctgcctgcc cagntcaagc 60gattctccat catgttggcc aggctggtct caaattcctg aggtgatctg cccacctctg 120cctgccaaag tgctgggatt acaggtgttg agcgatagtg ctcggcctat tatttctttt 180taaatctttg gtagaattaa tcactgaaac tatntgtgct ttttttgnng gaaaaattat 240ttattttaaa gacagggtct tgntctgttg cctgtgctgg antgcagtgg tgcaatctca 300gtttactgca accttgtgcc aacctactgn caagtgatcc tactgnctca cctccnagta 360ncttggatta caggcacgcg ccaccatgcc cngntaggtn ttgnattttt aggagaaacn 420gggtttcatn atattggnca gcnnggcttg agcttctgaa ctcaantgat ccnccncctc 480ggcctnccaa acactgggat tacaggcgtg agccctcccc tgntgatacg nagnggtttt 540aanaagattn tcttcaantt ngtttaaaaa ttctaatttn ngaccatttt tnctgcccgc 600ggcgnnaaag gcnaatcn 618 2 640 DNA Homo sapiens misc_feature (1)...(640) n = A,T,C or G 2actttttttt tttttttttt tttttgagac agggtcttgc tttgtcacct gggctgaagt 60gcagtggcat gatcatggct cactgcagcc tcaacctcct aggctcaagt gattctctca 120cctcctcctc ttgagtagat gggacttaca ggcgcatgcc accacatgca gataattttt 180gtattttttg tanaaacagg gttttgccat gttacccaaa ctggtcccaa gctcctgggc 240tcaagagatc tgcctgcccc aacctcccaa agtgctggga attacaggca ttgagccacc 300acacccagcc tgattgtttc ttctcataac tcaactctac tgntgatcct ctttaatgaa 360ttttantttc aagtcattct acttttccac tccaaaattt tgatttgggt cttttaaata 420aattttattt attggaattc tttatttggg gagaaggtat catatattcc tttanttctt 480ttggcgngct ttcttttaac tctttgatat ttataatagc tgntttgaaa gctttttntg 540gtaagtccaa cattngggnc ctcaangctt ttttaatgct gctttttttc ccctattatg 600gnaaacttcc agttatttta tgctaataag gttcggaaaa 640 3 635 DNA Homo sapiens misc_feature (1)...(635) n = A,T,C or G 3acactagcat aaatgaagat taagaaataa gtctttccag attattttta ctcaagaatt 60tgtttcagtg ctaggcaagg atgatcaatt ttagtttgca tatgaagact caaagggaga 120tgattaaaag cacgtaactc tttgactcac ccctagaagg tctttgatga ggcccagcaa 180tctggaaaat tatgatataa tattacacaa tgattattta acaatatttt agaagtaact 240gccatttggg ggtcacagaa caatactaat ctcaattatg ttacccatca acaaattgaa 300tataattaaa ttattttcaa aatatatggg ttgagattat tttccaatta aaattgccag 360gtgaggaaca gcacttttcc attcgctgct gaatgtgatg aaatactgga tagtcataga 420gggtctaccc agatgtccct ttgggagaag tgttgtgggg gaaaatgggc tggttgtgtg 480cacccaaact accctttaag aacttggtgc tggagccatt aaaaataatt gngctggtct 540tataaatatg aaaaactttg ggaaatcctt gtgacatcga tgcanttggg ttgggaagtt 600cctgataaaa atatctaaaa atacacccat tgaaa 635 4 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C or G 4acgcggggac taagacctca aaagcacagg cgacaaaaat aaaaatagac aaatgggaat 60taactaagaa gtttctacac aacaaaagaa ataatcaaca gagtaaacag ataatttcca 120gaacggaaga aaatatgtgc aaactattca tccagcagtg gacaaatacc cagtatatac 180aagaaactca aacaacaaca ataaaagaca aatcatcccc ttaaaaggag ggcaaaagac 240aagaacagac atttttcaaa agaagatata caaatgacta acaggtatat taaaaatgca 300caacatcact aatcatcaga gaaatgcaaa ttaaaaccac aatgagatat catcttaccc 360cagtcaaaat ggctactatt aaagagtcaa aaaataatag atcttggcca ggacatggat 420aaaagagaac tcttacatac tggtggtagg aatgcaaatt aacacagcct ctatagaaaa 480cagtatngag attgctcaag aactaaaaat agagctatca tttgcccanc atnccctgnt 540gggttctacc caangaaaag aaatcatggt caaaaaaaaa aaaaaaaaaa aaagtncttg 600gcgggaccct aaggggattc acccctn 627 5 411 DNA Homo sapiens misc_feature (1)...(411) n = A,T,C or G 5cgaggtacgc ggcctggcca acatggtgaa accccgtctc taccaaagat acaaaaaatt 60agccaggtgt ggtggcatgt gcctgtagtc ccagctactc aggaggatga ggcaggggaa 120tcacttaaac ctgggaggcg gagattgcag tgagccaaga tcgcgctatt gcactctagc 180ctgggtgaca gagcaagact ccgtctcaaa aaataataat aataaaatga aaataatcag 240ctgggtgtgg tggcatgtgc ctgtagtccc agctactcag gaggatgagg caggggaatc 300acttaaacct gggaggcgga gattgcagtg agccaagatc gcgctattgc actccagcct 360gatgacagac ctagactccg tctccaaaaa aaaaaaanaa aaaaaaaaag t 411 6 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 6accaagtcac cagacacaag taaatatggg ccttggggct tcttttcttg ctgacgcata 60ttcacacagg cagcgtgtgc tgtgtgtgtt tacaactgtg tttcttagtc ttctattcag 120agtaataaca gcatgacttc cctaagatct gattcagaga attgaaatat gccctgagaa 180aacataagag gtttttctgg agaagtgtcc caagggtaat attaattgtt caaggatgtt 240tcggaaaaag ttgcaatcat cactgtggca aatgaatcta gggagaggaa gcatgagtta 300tttaatgtca gttactcctt tccgtaggtt tttgcctttt tttggacttt acacacagcc 360catttgctat gaaactatca gctcaaatag cangctttca ngcaggccaa caatggcaga 420ctgcattctt nctactttnt ccaatcatat ttatcaagtc ccattgggag aatactttca 480gtagngctca aantacccgc ntncaattgg aactgcangg aaccnttcag aaataacnct 540tnaagaaaga aataaccctt canggaanac cctttnggnt tcactctann tggggttnac 600aagaaa 606 7 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 7ccgtggcccc ggccgaaggt ccnctgganc cccgggtggt aattggctgg aggtaaatgg 60tggtaangta ttaaccattt ctatggaaat gnccctttgg ggccctcctg gattttaaaa 120tggtcccctg gtttggacnt ttctattaaa gaaatggnca ttttacctaa aatgccnggt 180ctaccttatt aaagancaaa tngnntattn gaccttaaaa taggcatttt tcctaatcat 240aatctggccg gcttaacccc aatcaagata attgggtgcc cnttatgaat ttgaagttag 300tgatagcctc cttgtaaggt gctaccctna tggggataga gaccccagct actantaatt 360ngggaaaatg gttaaggtat ttgggaaaag tactctttta aaaacatatt ggccacagaa 420ancctaggct gaattacnng gattgataat tttgnaanta atttcntana atgggcnngc 480tggatgaaaa aatggcctcc tcnttttccc tggaaccagc ngctttttgc ctaaacntta 540ncctttttaa gttgaaccta gggaccacct aatnggcntc acaattccct ttttcctttc 600cttttttttt gcccaagggn 620 8 263 DNA Homo sapiens 8gcgtgggtcg cggccgaggt accacttttc ttattgcaac tcaacaagtg gcaattggtg 60atgaaaagtc aagtggggaa cccagtctgt ggggaacaaa tggaataact tacctgtcac 120cttgtctaac cgggatgcaa atcctcaagt ggtattaaaa agcatacagt gttttataac 180tgtagttgtg tggaaagtaa ctggtctcca agaacagaaa ttactcagcg cacttgggtg 240aatgcccaag aaataatact tgt 263 9 590 DNA Homo sapiens misc_feature (1)...(590) n = A,T,C or G 9acaacagggt tcttgcatca agcttcatgc tttcccagac atttactcaa gggaacgtgg 60gagagggagg aggaggaggg gagctgggag tgataagcag atgttacaca tgtttttcct 120ggaaagatca ccccactttt tctaatttcc cagaattaaa agaatgtatt ttatctgtat 180taccatggaa attactagta acactggatt tttttccctc ttttctaaag tttccaaaaa 240ctttcaaaag tgttcaaaga aattttcttg aacaatttta atatgtttga tttctcattt 300ggggctggaa tatttgtatt ctttttaatt tttttacttc atttattaga agaagtttct 360aatatgtgta ggaatacaat tttaaatgta agattatata gatgtagata tagatagata 420gatatatgta gatatatnga tttatgtcnc aatatcactn taaggcattc ttcttccatc 480cttttatatc tncccaaact ggtntnatgg gacctgtcct gcctgtaggt aaaanccttn 540taatttccct gaaaggctac cnctttctan ggggncaacc aattgggagn 590 10 609 DNA Homo sapiens misc_feature (1)...(609) n = A,T,C or G 10cgaggtacgt ttttcaatgt tttaaaaaat tgaaagggag tataatgttt cataacacat 60gggaaattat gtgcagctca aatttcaagt atccataaat aaagttttat tggaacacag 120ctacgctcac tcattagata ttgtctatgg ctgtttttgt gcaaaatggc aganttgggt 180tcagagttag caacagagag cttgtagcct gcaagcctag agtatttact atctggattt 240ctacagaaaa aaaaaattat tgccccctgc catacagtct gactgatagc ctgagaaagt 300atgcattaaa agaaagttac ctaccctgac cccatgagaa tgaatttgaa aagaaccnag 360atgtggtaga agcagatagg ctatgaaagt ttcagaaggg tancatcact gtgggcnagg 420atattcaaga aaagacttca nggaaaatgt nggggtttga actggncttg agtaggagtt 480naacttangg gaactggntt taggtngcca ctttaaggct gtcaaanatc atggcccaac 540attcantttg gcccaaattc cccangngcc ttaaaaattt ggacatggct tgggttgggg 600gncaccctt 609 11 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 11acgcgggatg tagtcagagg aggccctgac atctgcaggg cagcatgggt caaaccaaaa 60agacttttct gaggttgggc gcagtggctc acgcctgtaa tcccaacact ttggaaggcc 120agtaggggcg gatcacctga ggtcaggaga ttcgagacca tcctggctaa cacggtgaaa 180ccccatctct actaaaaaaa atacgaaaaa aattagccag gcgtggtgac gggtgcctgt 240agtcccagct actagggagg ctgaggcagg agaatggtgt gaacccggga ggcagagctt 300gcagtgagcc gagatcaggc cactgcactc cagcctgggc cacaagagcg agactctgtc 360ttaaaaaaaa caaacaaaca aacacacaca cacacacaan aagacaaaaa taattagcag 420ggaatgctgg tgcatgcctg tatcccaact ctcaggaggt tgaagcagga gaatcacctt 480gacccatnag caatgttcat gaacttagnc cngccntgga cttcancaag gcaccgagta 540aganttcntt tnaaaaaaaa aannnaaaaa aaagtcct 578 12 581 DNA Homo sapiens misc_feature (1)...(581) n = A,T,C or G 12actttttttt tttttttttt tttttttttt gggacggagt cttgctctgt tgcccaggct 60ggagtgcagt ggcgccatct tggctcacta caagctccgc ctcccgggtt cacaccattc 120tcctgtctta gcctcccagc gcccgccacc gcacccggct aattttttgt atttttagta 180gagacagggt ttcaccatgt tagccaggat ggtctcgatc tcctgacctc gtggcccacc 240tgccttggcc tccaaaagtg ctggaattac agtcgtgagc caccacgccc ggcctaaacc 300atttctcttg acaacactct ggattttatt tctggccaga taccatttat caattttacc 360atcaagaata agataatcaa aataataatc aagttttata ttagacttat gaagattctt 420gcacctttga aattacagct atctcactag ttnattctcc tctctcatat tttattacng 480acntccagga agacaaccaa cacctttaaa agttggctga gcatttttta nggagaccct 540taggtaanag ggncctnggc gggaacccct taggggnaat n 581 13 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 13ggtactggaa caactataag acccctgttc agattaagga atttggtgca gtttcaaaag 60tagacttttc tcctcagcct ccatataatt atgctgtcac agcttcctca agaattcaca 120tttatggccg atactcccaa gaacctataa aaaccttttc tcgatttaaa gacacagcat 180actgtgctac ttttcgacaa gatggtagat tgcttgtggc tggcagtgaa gatggtggag 240ttcaactttt tgatataagt gggagggctc ccctcaggca gtttgaaggc catacaaaag 300cagttcatac agtagatttt acagctgaca aatatcacgt ggtctctggg gctgatgatt 360atacagttaa attatgggat attccaaact ccaaagaaat ttttgacatt taaaggaaca 420ctctgattat gtgangtgtg gatgtgctag caaactttaa tccggatctc tttataacca 480gggacatatg atcatactgn gaagatgttg gatgcncgaa ccnattgaaa agtggtcttt 540ccgttgagca tggccnncag tngaaantgn cctacttttc cccttggaag gctttggggt 600annangg 607 14 599 DNA Homo sapiens misc_feature (1)...(599) n = A,T,C or G 14ggtactttca aaatataaca attttcgttc tcccatataa cagggggcta acaagaaaac 60caaaaataaa taaaaagaga aaatttaaaa ataagtaaaa aataaaaaaa tatttttaaa 120aagcagcctg ggcaagagaa gtgggtgggt ttaggagaat ccctttcgaa aaattcagag 180cattattatt aatcgttctt aaattaaatg cagggccaag catgctgcac gtggaatctg 240gacaattttt tgataaactt taaggctgct aaataattta cagaaactgt gaatgcattt 300tcattttacg aggcaaaaga gaaaatattc aagattgcat agcaatttta ttttttgaaa 360tggttatcct aaagaatttc cttaaattca gattttgcaa aattcctact ctncaagtca 420tcaagtgaac actaaaagca actttctcgt gaatcagtgg acttttacga ggcatgcatt 480tttcataaat ctaggccaag tgacctaatt gngattaaat cttaatcatc ctgngattct 540ggctattaan atgggtttaa ancngtaaaa atnctttnaa aaagccgtta cttnccgan 599 15 457 DNA Homo sapiens misc_feature (1)...(457) n = A,T,C or G 15ggtacttttt tttttttttt tttttttttc gaaatgaaca aatatttatt tatcttttat 60aacaagtaag gcaatgttgc ttaaaggaag acaaacaaac ataaaagatt ccgttgacaa 120tgcatttttt catntgttcg gcacaatgct tttgtcataa tggagatgtg acagcaaact 180ttccaggaca ttcagtcttc ggnggcagca cttagggcan atgactggcc gctcaaattc 240tctatnttgt ttcaggacag tggaaaagct tatanatgag gccaaagcac caggtaggtg 300gaaggttctt gtatcggttc gaaccccgac agcgcgccaa cagacaacac naggcagtgg 360ggagcaacat gctgttttaa tgancgcctg ggtgcangcg tgctgaggct gaaaatggca 420taacccccgc gtcctgccng gcgggcgttc aaanggn 457 16 643 DNA Homo sapiens misc_feature (1)...(643) n = A,T,C or G 16ggtacaatct agctgaaatc atatacaagt aagtaggtgt ggacttttac tgctgagcta 60aagtttatgt ttatatatgt tttattcttt aagctaaaca aacattcaga taacattcta 120tgcatttttt gaagcatagg gttagtaatg aggacttaga ttttttaatt aaacaattca 180gtaactatat aaaaagaaaa ggagtccctt atgaataaat attaaaatta aaagaaatag 240gcaactataa aagtaagtat ttttaataat ggcattgatt ttagtaagaa atcaattagg 300ctgggctgga aagaaaaact ggcttaatat aaagtagttt taatatggca aatattcttc 360ttaaaattgn ggccctggaa tatcatttct gcctattgct gatgctaagg natcaactgn 420gccaagtatt gggctgntcc acaggtggga angagtagca acattttgng gatttttttt 480tttttttaaa accggagaat acccggccag gggntcaagn ctgnatccac antttgggag 540nttagccgga naanccttgg anccggagna aaggttnaan gagncaaaat gngccatggn 600ttccanctgg ggacccgggg gnaactcttt taaaccnaaa aat 643 17 336 DNA Homo sapiens misc_feature (1)...(336) n = A,T,C or G 17ggtactttga taaatgtaga aagattattt aattctggct tggtaccgtg gctcatgcct 60ataatcccag cacttcagga ggctgaggtg ggtggatcac ttgagctcag gagtttgaga 120ccaggcgaaa ccctgtctcc acaaaaaatg caaaaattgc tggacatggt ggcacatgcc 180tgtagtccca gctacttgga aggctgaggc aggaggatag cttgagccca ggaggtcaag 240gttgcagtga gccgagattg tgccactgca ctccagcctg ggcaacagag caagaccctg 300cctcaaattt aaaaaaaaaa aannaaaaaa aaaagt 336 18 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 18ggtacactct tcttcgcctt tgagtgccgc tacctggctg ttcagctgtc tcctgccatc 60cctgtatttg ctgccatgct cttccttttc tccatggcta cactgttgag gaccagtttc 120agtgaccctg gagtgattcc tcgggcgcta ccagatgaag cagctttcat agaaatggag 180atagaagcta ccaatggtgc ggtgccccag ggccagcgac caccgcctcg tatcaagaat 240ttccagataa acaaccagat tgtgaaactg aaatactgtt acacatgcaa gatcttccgg 300cctcccgggc ctccattgca gcatctgtga caactgtgtg gagcgcttcg accatcactg 360cccctgggta gggaaatgtg ttggaaaaga ggaactaccg ntacttctac ctcttcatcc 420tttctctttt ccctccttac aaactaaggc tttngctttc aacatcgcta tgtgggccct 480aaaatctttg aaaattggct ttttggaana cattgaaaga aactcctgga aactggtcta 540gaaagnccta attgcttctt tacactttgg nccnncnggg actgatggga tttcanactt 600tcttgggact ttna 614 19 296 DNA Homo sapiens misc_feature (1)...(296) n = A,T,C or G 19actttttttt tttttttttt ttttttttgg gatggagtct cactntgttg ccaaggctgg 60agtgcagtgg cataatttcg gctcacttca acctctgcct cccgggttca agcaattctg 120cgtcagcctc cggaggagct aggactacag gcatgcacca ccatgcccaa ctaatttttg 180natttttagt agagatggag tttcaccata ttgaccaggc taggctggtc ttgaactcct 240agcctnaggt gatctgccca cctnagcccc ccaaagtacc tcggccgtga ccacgc 296 20 565 DNA Homo sapiens misc_feature (1)...(565) n = A,T,C or G 20accaattata atgcattatt atgaaatatt taaaatgggg aatccaagat gacatagttt 60ttaactcatc cacatactgg aagtttagag aaactcagaa tttcttattt ctttttcttt 120ttcctccata gcataaaagc tttgctaata agaataaata tatatattgg agttttagtg 180tttgatcctg tgatcagttg taaccatgtg tcataaaact ctctcacaga ttccatcttt 240cccaaatctt ctgatcataa cacagattgc catatagact tcccttgtaa ggagaatatg 300ctggccataa ggcaagcana agtgaacttg cagtttcact tcttggaaat taatgcattt 360gcattgactt ctataannta atctctcctg aatttttttg cttagtcaac ttactgtgtg 420caaagncaac agnaaattgt ctttggttna acttttaaca ggncaattta taaattggtt 480tgaagaagcn tcccnaaatt ttttattgaa ggctgaattc aagcctccnt taaaatggnc 540atngnataan gggaatttat tgtng 565 21 582 DNA Homo sapiens misc_feature (1)...(582) n = A,T,C or G 21ggtactggaa caactataag acccctgttc agattaagga atttggcgca gtttcaaaag 60tagacttttc tcctcagcct ccatataatt atgctgtcac agcttcctca agaattcaca 120tttatggccg atactcccaa gaacctataa aaaccttttc tcgatttaaa gacacagcat 180actgtgctac ttttcgacaa gatggtagat tgcttgtggc tggcagtgaa gatggtggag 240ttcaactttt tgatataagt gggagggctc ccctcaggca gtttgaaggc catcaaaagc 300agttcataca gtagatttta cagctgacaa atatcacgtg gtctctgggg ctgatgatta 360tacnagttaa atttatgggg atattncaaa cttccaaaga aaattttgnc catttaaaag 420aacactctng antatggnga aggtgnggnt tgtgcctaac caaacttaat tccgggatct 480tttttatnta ccnggattcn tttggatctt ncnggtaaaa aanggttgga tnccccnaac 540nnattgaaaa nngttctntc cnnttgacct nggccanccn ng 582 22 349 DNA Homo sapiens 22actttttttt tttttttttt ttttttgaga tggagtcttg ctcttgttgc ccaggctgga 60gcaacctccg cctcctgggt tcaagtgatt ctcctgcctc aacctcccga gtagctggga 120ttacaggtgc ccgccaccat gccgagctaa tttttgtatc cctagtaaag acggagtttt 180gccatgttgg ccaggctggt ctcgaactcc taacttcatg atctgctcac catggcctcc 240caaagtgctg ggattacagg cgtgagccac tgtgcccaac cctcttttcc tttttcaaat 300gtcaatggaa agttgattgg aaaggacaat ttggctacct tttggtacc 349 23 576 DNA Homo sapiens misc_feature (1)...(576) n = A,T,C or G 23acctgttctt ggagccaatg tgactgcttt cattgaatca cagaatggac atacagaagt 60tttggaactt ttggataatg gtgcaggcgc tgattctttc aagaatgatg gagtctactc 120caggtatttt acagcatata cagaaaatgg cagatatagc ttaaaagttc gggctcatgg 180aggagcaaac actgccaggc taaaattacg gcctccactg aatagagccg cgtacatacc 240aggctgggta gtgaacgggg aaattgaagc aaacccgcca agacctgaaa ttgatgagga 300tactcagacc accttggagg atttcagccg aacagcatcc ggaggtgcat ttgtggtatc 360acaagtccca agccttcctt gcctgaccaa tacccaccaa gtcaaatcac agaccttgat 420gccacagttc attaggataa gattattctt acatggacag caccaggaga taattttgat 480gttggaaaag ttcaacgtta tatcataaga ataatgccag tattcttgac taagagacag 540ttttgatgat ctcttaagta aatactctga ntgccn 576 24 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 24acttaaaata aagttaacaa ttacaacaga cccaatcaca gacaatacca gcgtagaaat 60attaactcca gaattatgac ttttatcagg agtaggagta ggagtaggag taggtgtagg 120atcaatgtca tcaggatttg cttgagggat aaacaaagtt acttgtgcaa tgttggatac 180ttttgatgtc aaattgcttt tatctatact tttaatggca ataaatatgt gggttgcatt 240ttcttctgag atattttctg gtttaaatgc aaagctttcc ttggagttgg cctcctttgg 300tgacagatca gtagtattta cttgaagagc atcatcaaaa ctgtctctta gatcaagaat 360acttgcactt attcttatga tataacgttg aacttttcca acatcaaaat tatctcctgg 420tgctgtccat gtaagaataa tcttatcctc atgaactgtg gcatcaaggt ctgtgatttg 480acttggtggg tattggtcag caagggaagg cttgggactt gtgatccaca aatgccctcc 540ggatgctgtc ggctgaaatc ctccangtgg ctgagtatcc tcatcaattc aggtcttggc 600nggttgcttc aattnccc 618 25 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 25acataccacg ctgggtagtg aacggggaaa ttgaagcaaa cccgccaaga cctgaaattg 60atgaggatac tcagaccacc ttggaggatt tcagccgaac agcatccgga ggtgcatttg 120tggtntcaca agtcccaagc cttcccttgc ctgaccaata cccaccaagt caaatcacag 180accttgatgc cacagntcat gaggataana ttattcttac atggacagca ccaggagata 240attttgatgt tggaaaagtt caacgntata tcataagaat aagtgcaagt attcttgatc 300taagagacag ttntgatgat gctcttcaag taaatactac tgatctgtca ccaaaggagg 360ccaactccaa ngaaagcttt gcntttaaac cagaaaatat ctcagaagaa aatgcaaccc 420acatatttat tgccnttnaa agtatagata nagcaatttg acatcnaagt ntccacattg 480nacaagtnac tttggttatc cctcagcaaa tctgatgaca ttggatctac tctactctac 540ttctanttct gaaaaaggat aatccggngt aaattttccc tggattgctg ggatg 595 26 361 DNA Homo sapiens misc_feature (1)...(361) n = A,T,C or G 26actttttttt tttttttttt ttttttctga gcatattata tctaattttt gaaggttgta 60ttttctccct tgttttaatt ttctgcanat acttttttct tttttacttt ccccaattag 120tttgtttctg actttcttcc tcaatctctc ctgaaccatt gtttnttttt aagatcagag 180cagattctta ggaactttta aaactgtatg tgggtgggat tgtcacctan agtgcttttt 240tggagagtaa ttggatggng tgataattaa ttttatgtgt caatttgaca gggtcttggg 300gtgtccagtt atttggttaa acattatttc tgggtgtgcc taaaagggtg tcccgcgtac 360c 361 27 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 27acctgttctt ggagccaatg tgactgcttt cattgaatca cagaatggga catacagaag 60ttttggaact tttggataat ggtgcaggcg ctgattcttt caagaatgat ggagtctact 120ccaggtattt tacagcatat acagaaaatg gcagatatag cttaaaagtt cgggctcatg 180gaggagcaaa cactgccagg ctaaaattac ggcctccact gaatagagcc gcgtacatac 240caagctgggt agtgaacggg gaaattgaag caaacccgcc aagacctgaa attgatgagg 300atactcagac caccttggag gatttcagcc gaacagcatc ccgaggtgca tttgtggtat 360cacaaagtcc caaacctttc cttgcctgac caatacccac caagtcaaat cacagacctt 420gatgccacaa gtcattagga taaaatattc ttacatggan gcccangaaa taattttgat 480gttngnaaag ntcaccgtnt ntataanaat aaggccagtt ttttgactaa aaaaagtttg 540aagagctttc aagaaancta tgatttgncc caaggggccc tccaggaagn ttgttttacc 600caaaattttn a 611 28 443 DNA Homo sapiens misc_feature (1)...(443) n = A,T,C or G 28cgtgcccaaa gcttggcaag ttttcggctt taaccacgca caccaccacc accaccatnc 60taaataactt actgcatcct caaagcctgt tttatgggga ttgcatggtt ttatttgaaa 120tcacgcctgt aatcccanca ctttgggagg ccaaggcagg cagatcacaa ggtcaggaga 180tcgagaccaa tctggctaca cggtgaaacc ctgtctctat taaaaaaaat acaaaacaat 240tagccaggca tggtggcagg cgcctgtagt cccanctact cgggaggctg angcaagana 300atggcgtgaa acttggaggc ggagcttgca atgagccgag atcgcacttg ctgcacttna 360acctgggcaa caaaacgaga cttcatntct nttttnnaaa nnnaannnnn nnnnnnnnng 420tcctttggcc cgaccacnct tan 443 29 403 DNA Homo sapiens misc_feature (1)...(403) n = A,T,C or G 29ggtacttttt tttttttttt tttttttttg gagtgcatat catcacccca acttcggttt 60tttacatttt aatttgtatt gnttttaatt tattttgagg caatgtctca ctatgttgcc 120caggctggtc tcaaatgaaa acaatgctat caatcacatt cttgcatagg atatgtgtca 180gtaatcctcc aaaatgaaca tganaaatgg aattgtcaag tcatagatta agtgcatata 240acttttgaat agatagtata aattttttcc ccaaatgaga attttatatt ctcactggca 300acatgaaaat agccatctct ctataatctt atcaaccctc gatagtgtca ttttttaatt 360tataattatg agtgaaaatg gtcctgcccn ggcgggcgct cga 403 30 615 DNA Homo sapiens misc_feature (1)...(615) n = A,T,C or G 30ggtacagtgg tagcatccaa atgggcaaac gtagtagcag gggcagggtc agtcaagtca 60tcagcaggca catagatagc ctgtactttg taatattctt cccacccttg agaatggact 120ttgtaagatc cgccccctgc ccacaaaaaa atttctccta actccactgc ctatcccaaa 180cctataagaa ctaatgataa tcccaccacc ctttgctgac tctcttttca aactcagcct 240gcctgcgccc aggtgattaa aaagctttat tgctcaccca aagcctgttt ggtggtctct 300tcacacagac gcgcgtgaca gaaaccactt gaagcccggg cgcggtggct caggcctgta 360atcccagcac tttgggaggc tgaggtgggt ggattacctg aggtcangag ttcgagacca 420gcctgaccaa catggtaaaa ccctgtctct actaaaaatc aaaaaaanta accnngggtg 480gtggnnggca cctgtaattc agttcttggg accttangca ngaaaatcct tgaacttgga 540ggcggaggtg catanttgaa acaaaccttg nctcaacctg gnaacaaaat aaaaatccgn 600tnaaaaaana aaaaa 615 31 485 DNA Homo sapiens misc_feature (1)...(485) n = A,T,C or G 31acgcggggat aagctacaac ataaacacat ctaggttctt gttcttagaa tacagcatga 60agaatttgct ttcttctttc ttcctaacat tttcatgtga gatccagaaa ggacacattg 120tctctggcca ttcgaagaaa gaaagaaaga aagaaaaaaa aggtatttag agacagagag 180agaaaaaggc tgaaatgggt tcgctgggtt ctaaaaatcc gcaaaccaaa caagcccaag 240ttcttctttt gggacttgac tcagctggga agtctactct cctttataaa ttaaagcttg 300ctaaggatat taccaccatc cctacaatag gtttcaatgt ggaaatgatc gagttggaaa 360ggaatctttc actcacagtc tgggatgttg gaggacagga aaaaatgaga actgtttggg 420gctgttctgt gagaacccna tnggctngtg tatgtgtgga cagtccttcg gcccgaaccc 480cttan 485 32 780 DNA Homo sapiens misc_feature (1)...(780) n = A,T,C or G 32cgaggtacgc gggtgtctag accttatgtc aaaataagcc caattgtatt aaagagtatt 60aaattgtatt aagaataaaa acacatggcc gggcacggtg gctcacgcct gtaatcccag 120cactttggga ggacgagatg ggcggattac aaggtcagga gattgagacc atcctggcta 180acatggtgaa accccgtctc tactaaaaat acaaaaaaaa aattgtccag ccgtggtggc 240aggtgcctct agtcccacta ctccagagct gaggcaggag aatgatgtga acccgggagg 300canagcttgn agtgagccng agatctcgcc actgcactcc ggcctaggcg acagagcgag 360actctgtctc anaaaaaaat aatgantaaa aaaanaagtc ctgcccggcc ggcgntcnaa 420nggcgaattt cancacatgg cngcngttac tatggatccn actcggtcca anctggcgta 480atcatggcat agnttttnct gtggnaaatg gtatccgtnc aantcnccna attcaaccgg 540agcttaannn ntaacctggg gcnatnnnnn nctacttcat tattgcntnc ntatggcgct 600tncattggaa ctnttgcnct gnntatnatc gcccnccngg aaagnnttnn ntgggncctt 660ctctgttann atctnnggct tngttgggag gntnctntna gnggntngtt tnatnggtcc 720ngnaaatttc agcctangnc antnagcctn ttgnttaatc tccnactnna aaaaataang 780 33 742 DNA Homo sapiens misc_feature (1)...(742) n = A,T,C or G 33acataccagg ctgggtagtg aacggggaaa ttgaagcaaa cccgccaaga cctgaaattg 60atgaggatac tcagaccacc ttggaggatt tcagccgaac agcatccgga ggtgcatttg 120tggtatcaca agtcccaagc cttcccttgc ctgaccaata cccaccaagt caaatcacag 180accttgatgc cacagttcat gaggataaga ttattcttac atggacagca ccaggagata 240attttgatgt tggaaaagtt caacgttata tcataagaat aagtgcaagt attcttgatc 300taagagacag ttttgatgat gctcttcaag taaatctact gatctgcacc aaaggaggcc 360aacttcaagg aaagctttgc atttaaccan aaaatattta taagaaaatg cacccacata 420ttataccatt aaaagttnga taaaacantt tgcctcaaaa gtttccacca tggacaagta 480acttggttat cctnagcaat cttgtgcctt gattactcnn ctctattcta tcctgtnaaa 540gcntaatctg agtaaaattt nccctggntt gtggattggc tngtnatgta atttnttaag 600nctggcngac cnctaggnaa tnnccttggg cgttangncc gtngccantt gtattngtaa 660tttctngaat gtnntcnncn nnntaccngt aagnatgggn tnggnnatnn atnttttncn 720tnttnatnnn cntnnannnn tg 742 34 763 DNA Homo sapiens misc_feature (1)...(763) n = A,T,C or G 34ggtcaaatga ggaataatga ggaaacaaaa ccatacatac aagagggatg gcacagacct 60tgtgacaaag tggtcctgaa atttctggag gggaaatgaa taagaataac cgagatagtt 120atgcttggag gaagaggaag atcaaggtgt cctaacctac cagaaactaa gacttatgaa 180accttagtca ttaaaatatg tagtattagt tcagaaatag taaataaatc aatgtaactg 240aatggaacct gggaacaaat atagctacat gtaagatctg ggtatatgct ggaggtgaca 300taacaaatga agagaaacaa tggactattc aaagctgtgt tgctatcttt attggcaaca 360aatatgggaa aaaatnaaat gagatcctat tcacatgaat gacaaaaata aatgccatat 420tgattaaacc taaatatgac aaggaaggcc tcaaatttta gaaaaaaatg ccaaattnta 480cncattggga gataattcat taacaagacc aanaaccnta aggaaagatg ntaatttnga 540tatattaaga tttactatgt ttataaatca aggatagtcc cgcttaagan actttctttt 600atttttaatt aatattatta atatttgana cttgcttgnt tnggtgaacc ggtaatttgg 660tattnacctt ctccggttan gattnnctaa nccntgtgnt nngttgnncc ncncnatttt 720tntacagttn ttgcgcgnta ttncnggnng cccccnnngn ngg 763 35 767 DNA Homo sapiens misc_feature (1)...(767) n = A,T,C or G 35acaggggaat ggaatggaat ggaatgcaat ggaatggaat catccgtaat ggaattgaaa 60ggaatggaat ggaatggaat ggaatggaat ggaatggaat ggaatcaact cgattgcaat 120cgaatggaat ggaattgaac taacccgaat agaatcgaat ggaatggaat ggaacggaac 180ggaatggaat ggaatggaat ggaatggaat ggaatggaat ggaacggaac ggaatggaat 240ggaatggaat ggaatggaat ggaatcaacg cgagtgcagg ggaatggaat ggaatggaat 300gcaatggaat ggaatcttcc ggaatggaat ggaatggaat ggaatggaat ggaatgaaat 360gcaatggatt caactcgatt gcaatggaat ggaatanaat ggaatggaat ggaatggagt 420ggaataattc naatagaatg gaatggaatg gaatggaacg gaatggaccg gatggaacca 480attgtaatgg aatggaattg atggaatgga atggaatcac cctagtcaan ggaatgtatg 540gaccggattc aatgaatgga tattccgnat ggatggatgg gaatgaattg atgattggat 600ggatggatca ccatccatga agattgatga tggatgatgc cacccatgat gattatgnat 660tagngtnata tctncatnna ggatgntncn attatgngnt gatgacatga ntannccnnc 720nctttnancn tatttttttg ggnccccctc ccagttgntt taaannn 767 36 608 DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 36acatatagtc aacgaaatat tcaaagaata actttatata ctcttgttct ttaaattcta 60tcctctcttt cagaattctt ccatttaagt ttgggtattt tcctagtttc aacagatgaa 120cagaagactt cattgaacat tttgacagta agctactaga gaccaattat caactggtgc 180tacacatgct gtgttatctc ccttactatt aaactataac cctctcttgc tattttgttt 240catgcatcac caaccaaact tcattttttc taataaaaaa taaatatata aagaagacac 300tgacaggcat atattcacaa gatctcaact tcttaaaaca taagtatggg tatatttatt 360tctctcaaat gcatacnaga caataattac ncagcaacca atcttttgtt caacaatgat 420ttgantcata agcatttgga aattacataa tttcatatca atanccctgt tttttnaata 480cagaagtaaa aaanccccaa taaccaatct taaatttcna ttatcccctt acctccaacc 540tttnaaaggt cccaccgggc cttttccnac attaatttgg tnaaactggg gttnaaaacc 600gcctnccn 608 37 245 DNA Homo sapiens 37acagacatgg cggcggcttt tcggaaggcg gctaagtccc ggcagcggga acacagagag 60cgaagccagc ctggctttcg aaaacatctg ggcctgctgg agaaaaagaa agattacaaa 120cttcgtgcag atgactaccg taaaaaacaa gaatacctca aagctcttcg gaagaaggct 180cttgaaaaaa atccagatga attctactac aaaatgactc gggttaaact ccaggatgga 240gtacc 245 38 630 DNA Homo sapiens misc_feature (1)...(630) n = A,T,C or G 38actacactga attcaccccc actgaaaaag atgagtatgc ctgccgtgtg aaccatgtga 60ctttgtcaca gcccaagata gttaagtggg atcgagacat gtaagcagca tcatggaggt 120ttgaagatgc cgcatttgga ttggatgaat tccaaattct gcttgcttgc tttttaatat 180tgatatgctt atacacttac actttatgca caaaatgtag ggttataata atgttaacat 240ggacatgatc ttctttataa ttctactttg agtgctgtct ccatgtttga tgtatctgag 300caggttgctc cacaggtagc tctaggaggg ctggcaactt anaggtgggg agcagagaat 360tctcttatcc aacatcaaca tcttggtcag atttgaactc ttcaatctct ttgcactcaa 420agcttgttna gatagtttaa gccgtgcata aattnacttc caaatttaca tactctgctt 480anaaatttgg ggggaaaaat taaaaaatnt aattggccag gatnttggna atttgttata 540atgaatgaaa cattttngna ttaaaaatca nattacttnt aanctttgat aaantaaggc 600atggntgggg gtaattgggt tttttgttcc 630 39 626 DNA Homo sapiens misc_feature (1)...(626) n = A,T,C or G 39acagtggtcc ttttcagagt tggacttcta gactcacctg ttctcactcc ctgttttaat 60tcaacccagc catgcaatgc caaataatag aattgctccc taccagctga acagggagga 120gtctgtgcag tttctgacac ttgttgttga acatggctaa atacaatggg tatcgctgag 180actaagttgt agaaattaac aaatgtgctg cttggttaaa atggctacac tcatctgact 240cattctttat tctattttag ttggtttgta tcttgcctaa ggtgcgtagt ccaactcttg 300gtattaccct cctaatagtc atactagtag tcatactccc tggtgtagtg tattctctaa 360aagctttaaa tgtctgcatg cagccagcca tcaaatagtg aatggtctct ctttggctgg 420aattacaaaa ctcaaagaaa tgtgtcatca ggagaacatc ataacccatg aaggataaaa 480gccccaaatg gnggtactga taataacact aatgcnttaa gatttggtca ccctctcnct 540aagggagccc attgagccna nggngctaaa gcctcatact ccacctgaat ggttaggaga 600aaatttatcc caaaaaaaaa aaaaan 626 40 645 DNA Homo sapiens misc_feature (1)...(645) n = A,T,C or G 40cgaggtacgc gggcaggaca tttaaaaggt ttcagcagaa atcttatgat tatgtctgac 60ttgcagtatt ttatttgcct ctttgacggc tttttttttt tttttttttg agacagagtc 120tcacactgca ctccagcctg ggtgacagag tgagagactc cgtctcaaaa atgaatgaat 180gaatgaatga atgaatgaac aaacgaacaa ggtggtttaa tgtcagaaaa cttcctaagc 240atttgctccc caaacctttc atgtttttca agaagccttt attacataaa ggggaataga 300attaaaatgt ttctttataa gaaaaatata catatttgtg ttcttggccc cattaaaact 360aatcagtagt cctttggcca aaaaatagtc aacaaganaa ctgggtatga ntccnggcnt 420tactcctgnt cataagtgng gatgcntgtg tctganccna actgnctcaa ctngagctct 480tggggtataa caanaaaccc gngttttcat gaaacccctg ggccnttata aaaggtttcc 540cttggggggc ccaatgctta ttntngattn gggttccaaa anntngcaat tggnataggt 600gcttgaaata accccctttt agtnnaattc cnaccaaaac cntgn 645 41 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 41acgcggggct cttcacgagg tggaaacaag atggaggatt cggcctcggc ctcgctgtct 60tctgcagccg ctactggaac ctccacctcg actccagcgg ccccgacagc acggaagcag 120ctggataaag aacaggttag aaaggcagtg gacgctctct tgacgcattg caagtccagg 180aaaaacaatt atgggttgct tttgaatgag aatgaaagtt tatttttaat ggtggtatta 240tggaaaattc caagtaaaga actgagggtc agattgacct tgcctcatag tattcgatca 300gattcagaag atatctgttt atttacgaag gatgaaccca attcaactcc tgaaaagaca 360gaacaagttt tatagaaagc ttttaaacaa gcatggaatt aaaaccggtt ctnaagatat 420ctcctccaac tctaaanaan gaatataaat cctatgaacc aagctcgcct tttaacagtt 480tgattcttcn tactgatcca aaataagcgg ttttacctcc ttattgggag acattnttta 540aaaaagaaag tccatntntg naaccttttt ccaaaatttn tcagananac atgctgnttg 600gngacggctt aaaatt 616 42 259 DNA Homo sapiens misc_feature (1)...(259) n = A,T,C or G 42ngtacggtcg gtggcagtgc tattctgaga tctgtagatg cttagaatat cagtattttg 60gatgttgctg cattttacaa tttatttgga gtcttccttn attttcctcc agatatatga 120aaatatgcaa tacctgctta tatcatgtag aaaagcttag caattattaa tttttctnta 180tttcatttta tttgaccaaa gtcggtgctt cacttgactc antgtgtttt aggtgttngt 240ntttntacct ttccggtca 259 43 509 DNA Homo sapiens misc_feature (1)...(509) n = A,T,C or G 43acgagtgtat ttttgatggg aaggccatgc taaatctata aaacagatgt ttcctctccc 60aacagtggtc accagtagtt tcaacttttt ccccccagta gcatcaacca aacttagcat 120agtgattttt aactctttgc tcccacacgc actcatccca acttccccgc ttgccccact 180ccctgggggg aaataaccct gcctttaaaa taaatagcaa ccaagtgctc agttctatgg 240aaagtatgaa tatttatttc aggctttcga tcccaatcga tttcaaaaaa caaagtctga 300tttctctcct cagagcagct gaggcctcca tgttacgatg gtttcatgga gattgaagga 360gcacatttca tcaggcttag cacaaagtcc ctgatgccca ccatgtccca gccttagnaa 420aggaaagaaa cagaattcac caccatgggg ctgaacgaat gccacaccta atgtaaatga 480ncagctaacc ttggccaaat tgtggtttt 509 44 544 DNA Homo sapiens misc_feature (1)...(544) n = A,T,C or G 44ttttttaaaa gtgtcactna ntctttaann anatncatta ccattttttt tncaaantaa 60attacggttt taaanggaan acacatggna atntananaa ncaccgnnga annttaanta 120cctngggngc gancanactn anggcgaatt cgaaccaatg ggggcngnaa cnaggggatc 180ccagctnggt accaaaattg gcgtnatgat cgcaatagcg gtacctgtgn naaanggtta 240ttcnntngta aaancagann tcntnnaagn nngaccaaaa aangtaaatc ctggggtgcc 300taatgannga tntaaancna ttaattgggn tgcccacctg cnantttatc gttcaaaaac 360ccgttaaacn ngtgnaaaaa tgaatngcca acccntngga aaagccgnat cntttgggng 420cttttccttt ttggtcctna cncttcctan nngnnngttt gggnncggnt nagttcntaa 480aggcgnaaaa catttacaaa aataggggaa ancccgaaaa acatttaccc nagccacctt 540ntcn 544 45 630 DNA Homo sapiens misc_feature (1)...(630) n = A,T,C or G 45ggtactctct atcactgaca aatgcaggct ggattcttat tatatacaga gatggctcaa 60aaatggggtt tcagatcttt gtgacgaaat agaatactgt ttcatatttg aatcagaggg 120cttcttgttc tgagaaatag gttcaaaatc attggaacca ggaacaagaa tagcttattg 180ttatctgtga taacactgtt ttctaaacac aaggattttc ttttttatta atatgcaaca 240tagacattgc cataacagaa taataaacca catgtggggt tttaaaaatg aaatttggct 300aataggagca attcagctat ttttctatca agaaattggg tggggtggga tagaaagaaa 360aaccgggttc aaccccactt ctgcccccta accagctata tggcctggat ggagcattca 420acctttaata agggtcaatt tcntctgttn aaaagacccc aaacctggaa atcacnttng 480cctctccctg aaaataanaa ggctngattt ttggaataan aaacataatg nangctnggc 540ccaatggctc gccccgtaat ccaccctttg gaggccangc ggncggacac ttgaggtagg 600agttgaacca cccgccacct gggaacccnn 630 46 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 46tttttgactc ccaaagtcat tttatttaac aaaggggtca aggcagagga aagtttccct 60taatatcccc acaactgctc cacatgtctt ctgtggaaac acttcaccag gaactagctc 120aacactcttg ctaacaattt agtgtctata caggaaggct ggtgtctctg ttacaggtgg 180cccgttcctt aaagccttta gggttaatcg cagctgcact gagtggccaa gcagaccctg 240ttgggatgtg aaagcagttt gttaacaggg cccctggccg ggcccagagg ctgtcagact 300cancaagtaa cactgaatgt ccaaaaatac ggctgtgtta aactaacaag ccaatccttc 360tgctcagatc tctggataga aatgattttt cttttatcta tgggggaatg caatttcatc 420acaacccctt acataaacgc tcctgaaacc ctttcagtag acagcatttc aattcaaaaa 480ccaaaagtga aactatcttt gaaaacangg acctggctgg gaaaccatgc acacctcggc 540gaacactttt cccccccacg aacttggact ttntgggaag gtggcgggtt tttggcnaaa 600acattcttga agcntaggaa gg 622 47 253 DNA Homo sapiens 47ggtactttgg tttgaaaaca acacttagag cctccagata acttttaaga cttatttagc 60tttgtgggtg gtattttcat gcaaataagt aagggtgggt tttatatttt gtagaagttt 120tcggtcctat tttaatgctc tttgtatggc agtatgtata tattgtgtta agttcctcaa 180gaatctcctt aaaaactttg aagttaatac ttttgtgcaa ctgtgttttg aataaagcca 240tgacagtgtt aaa 253 48 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 48acttacatat cctacatttg actacattat ttccaaacca agtattccat ccaaaggaac 60atactgctat catagagacc aaggagggac tgtttaaagt tgccaaggtg aagcgagctg 120agaggctttg tcctcgtgcc agtaactctg aaatttctct taattcctgc tgtccaggca 180gcagaatgcc atggtttccc caagtaggta gctgctttag cagttaaagc ccaaatgtct 240gttctgttga tcaagaggtc tctgaatttc tgaagtggtg tttcgtttct ggtgactgag 300ttaatccttt acaatncctc ttgtaaagtg tgctaataga aagaatccac ctttcaaagc 360tgcagaacca naccgtgccc taaattgacc aaccgtanct gatgtgcctn angaagtctt 420ttgccaactg ccctgtgaan acccctnctt ccccccagct ngtggcttgc acactgaaca 480tttaaactgn gcaaagccgt gtagttataa nacagtaaat cccaaggctt ggttaantgc 540tgggnnaaaa ctggttggat anacttaact taaaacccct tacataaacn tnggaactcn 600aagaaaa 607 49 421 DNA Homo sapiens 49ggtaccactg gatgaggggc cgggacatac tgactgcccc tttgacccca caagaatcta 60tgatacagcc ttggctctct ggatcccttc tttgctcatg tctgcagggg aggctgctct 120atctggttac tgctgtgtgg ctgcactcac tctacgtgga gttgggccct gcaggaagga 180cggacttcag gggcagctag aggaaatgac agagcttgaa tctcctaaat gtaaaaggca 240ggaaaatgag cagctactgg atcaaaatca agaaatccgg gcatcacaga gaagttgggt 300ttaggacagg tgctgttccc gagactcagt cctaaagggt ttttttccca ctaagcaagg 360ggccctgacc tcgggatgag ataacaaatt gtaataaaag taacttctct tttctttcaa 420a 421 50 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,C or G 50ggtacttcag tattgcattc tattcctctt aatgttttta tgggatctcc agggaaagag 60gaaaatgaaa accgtgatct aacagctgag tctaagaaaa tatatatggg aaaacaggaa 120tctaaagact ccttcaaaca gttagcaaag ttggtcacat ctggtgctga aagtggaaat 180ctaaatacct ctccatcatc taaccaaaca agaaattctg agaaatttga aaagccagag 240aatgaaattg aagcccagtt gatatgtgaa cccccaatca atggatcctc aactccaaat 300ccaaagatag catcttctgt cactgctgga gttgccagtt cactctcaga aaaaatagcc 360gacagcattg gaaataaccg gcaaaatgca ccattgactt ccattcaaat tcgtttattc 420aaacatgatc aagaaacgtt ggatgacttt aaaaaanatg ccntaaggac anttgtgatt 480tgcaggtggg aagatnaaca gttcatatcc actgaatgaa atgcatcttg tggaaganct 540catgnatnaa ggttaatggc tgaaatgaaa actccaaaag aaaccaaaaa ataccggccc 600ctttgaaatt caggganncc tatg 624 51 632 DNA Homo sapiens misc_feature (1)...(632) n = A,T,C or G 51ggtacgcggg ggaaacggaa gtgagcggcg gggtcgactg acggtaacgg ggcagagagg 60ctgttcgcag agctgcggaa gatgaatgcc agaggacttg gatctgagct aaaggacagt 120attccagtta ctgaactttc agcaagtgga ccttttgaaa gtcatgatct tcttcggaaa 180ggtttttctt gtgtgaaaaa tgaacttttg cctagtcatc cccttgaatt atcaagaaaa 240aaatttccag ctcaaccnaa gataaaatga attttttccc cctgaagaaa cattcagggc 300tattttgctt cccttaaaat accagaatgg gattcaaggg cagtgccacc aggtcaaccg 360ctttcatttc tttcaagcct caaatctttc acttgaatgt ttgaagggta atggatgaag 420acctattgga attgagggat atctttaatg atccgcccca aaccgaatcc ttggaaaagc 480cacccttgat ggtggaatat aaccttggtt actgaatatg tgcctgtcat ggaaccgagg 540ccgcatctgg ttatagcatc tttgacctgc cggccgcccc aaaggcgaat ccacncctgc 600ggccgttcta tggaccaact cggnccaact gn 632 52 623 DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G 52acttttaatg gtgggaattt acagtagaag catcctttgc tgagttatac attcctttat 60caatctcttt tgatacaaca tttaaaacaa gtagcttcaa gaaaccactg gtgttttgag 120gatagtattt ctaaatagca ttcaggaaca gagtattatt gcacagatct gaagatcaaa 180aaaaagctca aggaaataca gatcggaagt gctgatgagt tatatttatt gaaaacccaa 240cttttaagga agtgctaaga tcagtcaccc atgtgaataa gaagccagga aaggaaagat 300ggggaaagcc canatcacca ggcttctatt aaggaggaaa gcaacagang aaacagtgaa 360agggaacaga aaggggtagc caagtgttac aaaaaanccg actggataac caaactncaa 420aaagngtatg ttggggagaa ctgaaangga aaacaaaata cttgactaat cntaagtaga 480aaaaagcagn tagagaaaac caaatatttc tggncctgtc acatacaact tcaaataccc 540ttatanaatc caaaaatgat gtgtgtaagg naaaatttat tgccntccga aaaataantt 600tntccaatnt gaaacaaatc aac 623 53 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C or G 53ggtacgcggg gtcgcatgcg ctgtggctaa tgccgtaggc tctttcaggg ctgagccatc 60ctgcgtgtct tgcgctcggt ggaaatgccc agccgaggga cgcgaccaga ggacagctct 120gtgctgatcc ccaccgacaa ttcgacccca cacaaggagg atctaagcag caagattaaa 180gaacaaaaaa ttgtggtgga tgaactttct aaccttaaga agaataggaa agtatatagg 240caacaacaga acagcaatat attctttctt gcagaccgaa cagaaatgct gtctgagagc 300aagaatatat tggatgaact gaaaaaagaa taccaagaaa tagaaaactt agacaagacc 360aaaatcaaga aatagtcaac ctgatttcac ataacaatgt gtggcatttg ttgttctgta 420aacttttctg ctgagcattt cagtcaagat ttaaaagagg acttactata taatcttaaa 480cagcggggac ccaatagtag taaacaattg gtaaagtctg atgttaacta ccagtgntta 540ttttctgntc acgtnctaca cttgangggg gtttgactac ccancctgtg gaagaagaaa 600gaagcaatgn ggttctatgg atggaga 627 54 565 DNA Homo sapiens misc_feature (1)...(565) n = A,T,C or G 54ttttccttga gtgctccctt ttatgtcatt ttattttctt ttatgcagac cagtgggggg 60aaaatcccat agattcttct ggaaactgtc aagatgctgg gaagatgaat gcaaaactta 120catagattgg gatgtccaca gtttggattt tcaaggtatg gcttttgcag gatgacgtga 180tcaacccaaa cttctgcttg atctggtttg tcctgaactc ctgccacttg ccgccaacca 240gggcctctgc tctgatctca tacttcacca ggcgtgccgn tcgcaggctg acgtggttgt 300gctcgtagac cgcagaggga gattccaggt ctgtgtgctt tattctctgc atgtaaaaac 360tataagaggt agtatcatgt ttgagtccct ttatcttaaa gaagaatcca tatagagcaa 420tcgttttcga ataagttgna ttctctgngt ctggcactgt gtccagtgct ctcanaggat 480gcangggaga anaccaaaaa gtntctgagc agtctcacat gggaaataaa atgtgtcccc 540ggtaccttgg ccgngaacac nctaa 565 55 451 DNA Homo sapiens 55acagagatga caagagaaag gcacaaatga ccggagtcag ggattgtggt gagggctcca 60catgaagaca gcatgttgga ggagaccaag ttgggaaggg tgacatgtca tacatcaaaa 120gttgccccaa gatagcaggt tataatgggc tagagagaaa ttagagggaa catctcttcc 180ttcacttgaa caacaccaaa aatagaagac cagagaatag aaggatggtg acaaatccca 240aaaaggaaat ggaggaggag ttcgtggaag ggcagaaaca ctttaatcct agagggaggg 300tgaggcactg ttgaaaagag aagcaaactt tggcaggggt ggccattctg ccttgctgag 360tcatgggctg agatacggaa gtcactttca atcattttct acttctccca gggcactcag 420acaaaatcag tgcaaggtat atggaagtac c 451 56 623 DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G 56ggtacgcggg gcttccgaga cgcactgggg gccggatgta gaatcctgct tatctgtgaa 60atgcagttaa cacatcagct ggacctattt cccgaatgca gggtaaccct tctgttattt 120aaagatgtaa aaaatgcggg agacttgaga agaaaggcca tggaaggcac catcgatgga 180tcactgataa atcctacagt gtttcactct tgttgcccag gctggagtgc aatggcgcga 240tcttggctca cggcaacctc tgcctcccgg gttcaagcaa ttgtcctgcc tcagcctcct 300gagttgctgg gattacagat tgttgatcca tttcagatac ttgtggcagc aaacaaagca 360gttcacctct acaaactggg aaaaatgaag acaagaactc tatctactga aattattttc 420aacctttccc caaataacaa tatttcagag ctttgaaaaa atttggtatc tcaacaaatg 480acacttcaat tctaantgnt tacattgaan aagggagaaa acnataaatc angaatacct 540aatatcttca gtngaanggc atcaaggttc tcttgaaaac ttnccggaat aatgaatntn 600ccnaagtcca aaanattttt aac 623 57 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 57cgaggtactt tttttttttg tttttttttt tttggtttct gtcctttaat tttttaacag 60aatatacaga gccacacaat acgatttcaa tttcaaatta tgggagatca tattcaaata 120tgcttaggtt tgacaagttg ctgttacaat actgagaact ttcatgaaaa cggtatttaa 180caatttttaa gataatcaaa tatctttttg ctacgtgggc caacgcatta atactaactt 240gtttaaaaat gcagtctttt agacttcaaa ttattataaa acaatatcaa gatcatatag 300atatacttcc tgattactca aaactcgttc cattctgatg gaggctgaag gtaaatgtta 360ttatacatta gaacatttca tgaaaccact tctcctttgc acttacctgt aaaagtcaaa 420aattaaacca caatttccta agacataact atttctagaa tacattggtg taatcataaa 480agactacnag taaattatca tttttatcta acacttttta ccacacacat ctttcctaaa 540aggaccnaaa aaaattggga atttggattc cttacataac aggactcata cttctgattt 600aataaattnc actcttttca ag 622 58 471 DNA Homo sapiens misc_feature (1)...(471) n = A,T,C or G 58ggtacttttt ttttgtttgt tttctagact taataaaagc ttaggattaa ttagaagaag 60caatctagtt aaatttccca tttgtatttt attttcttga atactttttt catagttatt 120tgtttaaaaa gatttaaaaa tcattgcact ttggtcagaa aaataataaa tatatcttat 180aaatgtttga ttcccttcct tgctattttt attcagtaga tttttgtttg gcatcatgtt 240gaagcacccg aaagataaat gatttttaaa aggctataga gtccaaagga atattctttt 300acaccaattc ttcctttaaa aatctctgag gaatttgttt tcgccttact tttttttctt 360ctgtcacaat gctaagtggt atccgaggtt cttaatatga gatttaaaat cttaaaatgn 420ttcttatttt cagcacttac atcatttggt acctgccngg cggccgntcg a 471 59 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 59ggtacataca caatcactca actggaacaa tcaaaaccat ctatgagtgt ggttattaaa 60aaataaaatt acgttcatac aatggtagaa aatgaaatgt ttttattaat ttgattatta 120atacaaaacc acacatatat gaattatata acctagtgtt atatatttaa aaatctttat 180gcttgcaact gaaatgtctc tactccaagg gaagtttctg atttttaatt ttcttatttt 240aaggaatcta ttatattcac aatgattaaa atgccttaca cataggcaaa aagcagaccc 300aatcccagca aacagaaaaa ccataagtct atcatatcac catatgtttc accatatagt 360tttgaaaaat aatcctattt gcagtttggt atgtcttcat atttatactt attatcaaag 420tgattgcata ttgaggcaca gagcttaaag aggaaatata tattacttat aggggaacca 480gacactgaaa caaggaatat caatcaatgg cttcaaacna aaaaaaaann nnnnnnnnnn 540nnnnnnggaa aaggaaaagt cctgncccgg cggncgttca aagggcnaat tcaaccactg 600ggggccgtac ttatggac 618 60 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 60actttttaaa ccctcccaac cagccctttc tcaatattca tcaaatctaa aacatttagg 60gggcaaaatt ctaacatgtt catggtatct tgcaaatagt aaaagcttta ttctgaagga 120ttataaacta gttttctcca ttttaactag cactattttg tggaaattag aaacctcttt 180tatttctctt cccaaaagta atacttatta taaggctgta gtatcaggtt aaggatacag 240ataaataaag ttcacttata tcttcttaca aatgtctggg ttttaatatg gttaatcact 300tatatacaaa tattacaact ttttagtgca agtttttgga agaaaacttt ttgataaaac 360actgtgattg atgtgacttt atttttaatt taaacgatga ggtggccaga agaaagatgg 420gtctaaaatt tctcccatga aagatgtaaa actatggctt ttttaaaatc aaaatttcat 480ctttaaaata atgggttgaa atctggatng gatctgaaca gaataatcac atttaggatc 540tatataaatc tcaactggag tntaactgaa ggaaataccn ngattttaag aaatatnttc 600aaaaan 606 61 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 61ggtacattct ggtatgaaaa catctcaaaa tgtaacaaca caagagtttg ggtcaagacg 60acccacccag gaggctgtaa aaactggttt gaactagaac tgtggaatgg aactagttta 120aaatatgaag cagctctaaa caccaagctt agagacattt gccctattag aaaacaaaaa 180tcattaaagc tacaaaataa caagtgcaaa catgctgaac ctgtttccag ggagtgacat 240tcccttctgc caacaggtcc caaactcaca cccacaaggt gtaactctct ttcctgttcc 300actagatttc ctttctctca tctcaaaggt cctcagaaat gacaatggaa aacgtatgaa 360ttgttgaaat ttaccctgtg gaccaattcc tgaagagata acagccacaa ctctgagatg 420attaagacat gcagtgttta cttgatgact ttctgnattt ctagaaaccc tcaaagcatt 480aaactgncta tttcaaaatc taaacttnct agcactttta ttatttggag taagcnnacc 540gaagacaatt tactggccca caggaataac cacgcttact tgtcaccata agtttacggn 600atggacattc actggaaaac 620 62 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 62gccgaggtac ataaatctgt gatcccattt cttattgcac cattcaggaa cactttatat 60aaatgagtgg ctttttattt catattatta gtagtatcat ggttccatta caggcctatt 120aacatcatac attgtcatta gtctttgaag aaaaaatatg taaatatata tgtgtaacat 180gagaatttct ctctaaagca gggcttaaaa ttttttggaa aagtttgaca aagcatacca 240catgaattca gatttacctc aatgctaaga attatgttta gttaggaaaa aggaaagtca 300ttttgacctc aggtagaaaa atagattgct ttgagtttta tgtagcttta gactttaaaa 360agttagaatt tattctgtaa ctaaaaatta tttgaaaaaa ttatgcctct ggtttaatta 420ttggtgatta cacactcttt ctcttaccct tgngtattga actatgtcca taatcaagtt 480gatgtggatc ctgaaaaatg gtatgaacat ctgatgggat tggcacatta ttttaaaant 540agcatctgac acttcaaaac tgtcantgng atgggttcac cataccacgg ntgaccntac 600attaaatttt nacn 614 63 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 63ggtacatata agagtaatta gttttattct ctctttttta taaaatcggg tttcagatga 60gatgtttatc ttagactatt ttagggaaaa attttacatg tttgagatgg tggagtaaaa 120agactgttaa acatttcttt taaaaaatta tttttacatt acaacaatat atttatgatg 180tgttcagatc aaaaatttaa cttctgtgtc ccagatctac tttcaaagtg agattttcac 240ttgtcagctt aaatttctga ctagaactaa catttgtgta tttttgtgct tagtcggaat 300acaaatttca cagtggattt ttgaagtttg tccttaaatt ggataaaatc aagtgattaa 360agttactaaa gagataaaaa tggtaatttc catttttaaa agtaatttgg ttgtgtttat 420agttatttgt acttcgagtc tcccttcacc atttccgacg gcatctacng ctcaacattt 480tttggtaccc cangctttca cggacttcac gtcattattg gctcaacttt cctcactatc 540tacttcatcc gccactaata tttcctttac atccaacatc ctttgacttt naagccgccg 600ctgatnctgc attttn 616 64 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 64ggtacagata tcattncttg tgtatgccat gacttgaaaa agtttgggaa gctctttanc 60aatatcagct aanaggatat gaaatcacag gtgatagcag ttgtcattca gtaatttcct 120acaagcagca ccccaaagga aatatagtcc taatctttac tatccacttc taaatttaat 180gtgaatttca tacatgttat tagttgtttc ctttataatt ttataaaaat tattcatcgg 240gagtttaact tccacttcca tgctatcgga tgtgttgggc tccatgcaag aacttggaag 300aaaaacaggc aggaatgcat ttgcataatg acccagatca tcattttctg caactgagaa 360ttatatttca tcattgcttc tagaagtctg caattcttta cttttctttg gtgcattatt 420atctangtgc ccatcactgg ataatgtgga gtgactagag aagtcatnta tcactggaag 480gncctgcccn ggcggccgtt caaaaggnca antccagcan nctggcggcc gttctaatgg 540gntccaactt ngggnccaan cttggngnan tcatggcnta acnngttccn ggggggaaat 600gntntccctc ac 612 65 599 DNA Homo sapiens misc_feature (1)...(599) n = A,T,C or G 65acaagctaca aaatagcatc tctttcatgg tatgtttgag tgtgtaattt tagtttcttt 60tctggttgta tttgtggtag tcagatgtgt tggattgatt ccaactggac agagtaagga 120attccagcat cctcttcctg cttgctcgtg ttaccccaca gatcaaaccc tcaattctag 180ttggggatgc tgtctagccc cacaccatga ctgaagcctt aagcactgtt gcgcctccat 240gtgctttggg tcagcaaccc cagtggtatt ctaccagagc attgtgggaa ggcagatgta 300tagtcaggtc ccaacagcaa attgttgggt gtgagagttc taaagtatag gggtgaaggg 360aaagagaang atatgaactc ctctgacctt aaccacattc atttaacttt tatgcctact 420taacaagaga acctggagaa aactatcgna ttcaagagat taatcaaaat cagggtttan 480ccagccatga ccgaaancnc cttccttaac ctcatcttgn anggctgnaa naattcannc 540ctaggatggt taanccagaa ccccngatga ttaantgtcc aaccttnatt tncatantn 599 66 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 66ncatgacctt tagtggaaga ttatttggtc atcaaatacc catatccaag tttccatggg 60gcctgggaat ttcctttcac ttggatagaa agtatatatt aggaaagtcc agttaataag 120tatttttatt taaaaaaaaa aaaaaaggaa aaaagaatca gcagaagtca agttgtctta 180agtcttaagg ctttctggat ttcttccttg gaggaggtca ggatcttccc aaggcctggg 240tcctcgaata ttcttccagt catcaaactt ggagtctttg attttctcat attccgactc 300taaagatatt ttattctctt tcagtttttt ttcaagctca ggatccattt tactcttcac 360agcatcatat cggatttgag aaaactcacg aagaccaaaa gaaccttcaa caatcagcaa 420caacatgggg actccatacc cagagtcttg gtcttgcgaa aagcacgcnt naaccgcggg 480tgccaacatg agtgaactct ttcatcggtt naaactccaa cnggcctacg caaactccca 540atttacaggt tangctttta ccaaacaagt ncctnggcgg gacnccctag gggaattcgc 600cactgggggg t 611 67 639 DNA Homo sapiens misc_feature (1)...(639) n = A,T,C or G 67nagaattcgn gcttncnagc ggtcgnccgg gcaggtacac tttacttaaa aactattaac 60agtttttcat gttgcactgg tggtaatttt gaacttggaa ttactgggtg ggaattccag 120gaaccacaga gtattgattt ttgctgccaa aatgctcttg aagcagatgt ccctgtgctc 180ccctggctgc ttctggctga aggggggagg tgtagactga agcttgggca ctcatgtgtg 240tcccctccca gtccccatcc tagtggggcc agtctcatta ggcagccata gataagcctg 300gaacttggct gcattagtga cttgatcctg gtatgaaatg catactgggt ataaagntgc 360tcaagnattt tatttccttg gccacaactt ccatagatgc caatggtttg atagcctcag 420tttctnaacg atgtcttttg gttacagtgc tcacttantg ngagtcaaga aatgcttgag 480ttaccagaaa cttcttantc aggttgagta acnttttacn ttcatgngta nctnnggcgc 540gaacaccctt anggggaatt ccacacactt ggnggccgta ctaanggatc caacttgggn 600ccaacttggg ggaaaaangg cnaantggtt ccttgngaa 639 68 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 68tcgaccggcc gcccggccng gnccttcccc atcactnnac tggnacnatc aaaaccntct 60atgantgngg gtattaaaaa ataaaattac gttcatacna tggtagaaaa tgaaatgntt 120ttattaattt gattattaat acaaaaccac acatatatga attatataac ctagtgntat 180atatttaaaa atctttatgc ttgcaactga aatgtctcta ctccaaggga agtttctgat 240ttttaatttt cttattttaa ggaatctatt atattcacaa tgattaaaat gccttacaca 300taggcnaaaa gcagacccaa tcccagcaaa cagaaaaacc ntaagtctat catatcacca 360tatgtttcac cntatagttt tgaaaaataa tcctatttgc agtttggnat gncttcatat 420ttatacttat tatccaagtg atgcntattg angnccnaag ctttaagang gaattttntt 480cctatngggg acccnaccct tgacccgaat tcatcaangg ntttaaccca aaaaaaaann 540aaaaaaaaat ggnaangggg ctcccttnaa anccccccca acctntttnt ttaacnagnc 600tnagcctttc a 611 69 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 69accaaagcat tacccgcatg gtagagaaca cactcgatta aaaatgttaa gctatctgaa 60aaataaaatg tgcaagtctt caggatggca caaaacaaag gtcaatgctt cttggggcac 120atttcttaga gggcttgctg agtgtgtaaa tataatcgac ttttgtttgt gttacatgac 180ttctgtgact tcattgaaaa tctgcacaat tcagtttcag ctctggatta cttcagttga 240cctttgtgaa ggtttttatc tgtgtagaat gggtgtttga cttgttttaa cctattaaat 300ttttattttc tttcactctg tattaaaagt aaaacttact aaaagaaaag aagtttgtgt 360tcacattaaa tgggtttggt ttggcttctt ttaatcaggc tttctgaaca ttgagatatc 420ctgaacttag agctcttcaa tcctaagaat ttcatgaaaa gnctntnact ttgaacccaa 480accanaatac ctcggccgga caccctaagg cgaattccag ccactggcng gccgtactaa 540nggatccanc ttggtnccaa cttggggnaa catggcnaac tggttccggg gaaatggatc 600cccncn 606 70 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 70ncgtggncgc ggccgaggtn cttttttttt tttttttttt ttttttttnn aaaangggta 60accttaaagg tttantggcc ccccaaangn aacctggggt taatggcttc nnattttaaa 120tttttggaaa ttaaaaaaat tacnagtttt aaatagccna tggctggnta tgttttcana 180aaacatgatt agactaattc attaatgggg gcttcaagct tttccttatt ggctccanaa 240aattcacccn ccttttgncc cttcttaaaa aactggaatg ttggcatgca tttgacttca 300cactctgaag caacatcctg acagtcatcc ncatntactt caaggaatat ccgttggaat 360acttttcana aagggaatga aagaaaggct tgatcatttt gcaagggccc caccacgtgg 420gcgganaaat cacttctaca ggttattacc tgganngtca aagntttctg naaaacanct 480tgctctcaac tggtttacca tttggtgctg gagctnacaa ccggtttaag gcccttggna 540anggtccaag ncccaanaaa ctttcccggt ccttccggng gccttnaagg gaatccnccc 600tgggggcgtt t 611 71 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 71nctgggaacn ccgaaggtgg aaggccnttt cataacattt cttgtggatc aaaccaccgg 60gacacctttt ttnccatcaa caggactagc gtcttgtcag tcttggtgac agtgacattg 120aangtggggg cccaccggtg ctcttggtac tttcccaaga ggtcctcatc ctgagacggt 180ctctacccat gtttaaccca aagagtgcag gccaggttcc ttatccttct gatgaaggat 240gagagaactc atttagaagt cagagcaaac tagggtctca gtattgagaa acgcacctgc 300canggaatca cagagacatc ggggtgcccg cgatggcctc atgaaccatg cctngacggn 360attcaggaac cctgcaaacg tgctttttga ctcattggnc agtgtgaatt ttacacaagg 420naaacctggt cnaaggcatt ngggaattgc tccaacnnat acttcctntt aggaacccaa 480ggaancaggt tcncgaattt tgaaaactgg gtntgaagtt ctttcttcct ttgggnacaa 540ggccttaaca aanancttgn ggnttccaaa tggncctggc cccacacc 588 72 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 72ggtacaaact tagaagaaaa ttggaagata gaaacaagat agaaaatgaa aatattgtca 60agagtttcag atagaaaatg aaaaacaagc taagacaagt attggagaag tatagaagat 120agaaaaatat aaagccaaaa attggataaa atagcactga aaaaatgagg aaattattgg 180taaccaattt attttaaaag cccatcaatt taatttctgg tggtgcagaa gttagaaggt 240aaagcttgag aagatgaggg tgtttacgta gaccagaacc aatttagaag aatacttgaa 300gctagaaggg gaagttggtt aaaaatcaca tcaaaaagct actaaaagga ctggtgtaat 360ttaaaaaaaa ctaaggcaga aggcttttgg aagagttaga agaatttgga aggccttaaa 420tatagtagct tagtttgaaa aatgtgaagg actttcgtaa cggaagtaat tcaagatcaa 480gagtaattac ccacttaatg gttttgcctt ngacttttgg gttaagaata tttttaaatc 540ctgnggctnc cttaattggc cgnttgncca ngggttccnn aaatgggttc n 591 73 581 DNA Homo sapiens misc_feature (1)...(581) n = A,T,C or G 73acgcgggtat ctgtaatttt tataattcat caattctgga atgctatata taatatttaa 60aagacttttt aaatgtgttt aatttcatca tcgtaaaaag ggatcatctc agagagaaca 120gcagtattct gcgtattttt aaaaatgctc tagagtaaca tttgaagtaa ttcactgtag 180tgtatgccag tcctagaaat aattttttta atttctggtg tctgtttcta atacactaac 240caagttttca aaatatattt acaaagatgc atctttaccc attattttaa aatgattaag 300gaggatagtt gcttcaggta acaagctaat ttttcaaata ttaggccctt acagaactat 360ttagtcaaaa agtaagatat tcctttaaaa tatataaccc aaagctttca gttaaaccat 420gatatatcac aaatactatt aaaatggtaa agagaaaatg caattgcant taatgatgcc 480caaatngtaa aatatngaga ttcaaaagct gggnctttat ttaggnggga tnccaatgnn 540aatgatactg gcctggnttt acctttacct tttaaaaaan a 581 74 599 DNA Homo sapiens misc_feature (1)...(599) n = A,T,C or G 74cgaggtactt tttccgcaca tgccttgtgc ctatctgagt attgatgcca tggatgtggc 60cggagaacag cagctggatg tggaacacaa cctgttcaag caacgactag ataaagatgg 120catccccgtg agctcagagg ctgagcggca tgagcttggg aaagtcgagg tgacggtgtt 180tgaccctgac tccctggacc ctgatcgctg tgagagctgc tatggtgctg aggcagaaga 240tatcaagtgc tgtaacacct gtgaagatgt gcgggaggca tatcgcccgt anaagctggg 300ccttcaagaa cccagatact attgagcagt gccggcgaag agggcttcag ccagaagatg 360caggaaccag aagaatgaag ctgccangtg tatggctttc ttggaaagtc aaataaggtg 420gcccgaaact ttcactttgc ccttggggaa ganctttcca gcantcccat gtcacntcat 480tgacttggca aactttggnc ttgacaaccn tnaccatgac ccactacatc ancacctgtc 540atttngggga ggactttcna gccttgggaa acccctngac cccccaatgg taattggcc 599 75 594 DNA Homo sapiens misc_feature (1)...(594) n = A,T,C or G 75acatcaaatt ataaatgcaa aacaggttca gatttcatct tttgtgattt cttttaaata 60ctattcattt ttatttaaat gcacagtatt tcccctatat tttagtcctt ccattcctag 120agacaaacca gttatttggt ggtgggaagt agctgaagca aagaaggaaa agtaatacct 180ttaacctcac tagcttcaag agtagacatt cttactagct caatttaaat aattgatttt 240aaataggaag aaaagaggat atatttaaga tacatagaaa ttatgatgtg aagtattcat 300gagaatctgt agattccatc aaaataagta ggaactcatc taaaattgtt ggatttaaag 360aggcactttt ggttatgatt caaatatggg gaatttgaga aatattcatt ttgnccactg 420gatggtcact attttactaa aanggnagct ttttatgggg ggactgngac tgaggtctta 480aagactgaaa gaagttgggg ggttcatttt cngtaccacc ttcnnggacc atttggacct 540ttggccggga acacccctaa ggngnaattn cngnccctgg gggccgtcta atgg 594 76 585 DNA Homo sapiens misc_feature (1)...(585) n = A,T,C or G 76acgcgggggg cggagtagca agtggccatg gggagcctca gcggtctgcg cctggtagca 60ggaagctgtt ttaggttatg tgaaagagat gtttcctcat ctctaaggct taccagaagc 120tctgatttga agagaataaa tggattttgc acaaaaccac aggaaagtcc cggagctcca 180tcccgcactt acaacagagt gcctttacac aaacctacgg attggcagaa aaagatcctc 240atatggtcag gtcgcttcaa aaaggaagat gaaatcccag agactgtctc gttggagatg 300cttgatgctg caaagaacaa gatgcgagtg aagatcagct atctaatgat tgccctgacg 360gtggtaggat gcatcttcat ggttattgag ggcaagaagg ctgcccaaga cacgagactt 420ttaccaagct tgaacttana aaagaaagct cgtcttgaaa gangaagcnc tntgaaggcc 480aaaacagagt acanaagttt ccnngttggc ttggattttg aaaattcnng aattntntat 540aacgggcttn tttaaaaagg atnggnttan gnacctttnt taaat 585 77 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 77ggtacgcggg agtcatattt atgaaaaaag gtttgtgttt tactcttgct agtgagaaag 60tgggacaaaa tatacttttg aaataaaatg ctatatggca cctaattatt ttttctttta 120aaatgcctta agttgcagtc tcattttgat aatcatttgc ttccagtgtt taaaaattaa 180aaaaagaatg gggagaaggt tatgagaaga gcattattaa gtttccaaat ttaatttgaa 240ttccaaattc acctagcaat aaaatctaat ttttaaaaag tatataaata taaaatgtat 300aaatgatgga tagatttttg tattgatttg caaaatgcag attatatttg ataggctata 360gtatgtagat attcctttta ggaatattac agctgtaaat tatatgagac ttgccagtca 420aatgctattt ggtttaaaaa aattattgca atctcaagtt aatggaatat ttttaaatcc 480cacattcaga gttaaaacct ngttttcaat gggtttttan tgtggcactt gnttatagat 540taatttttaa taacctgttn ggaancnggg ccttttaact ggtccttggg g 591 78 252 DNA Homo sapiens 78actgagaagt attttcagtg attcgaccca gaccagattt caacacatgg ttcccataca 60ggaaggactg ctctgcacca ggctttatcc aaactttata cttggcataa ggtgcaaggt 120aatccagagc tgtgacgtgc aaccgaaact tgtgggtttt agtgaatttt ccaaagcagg 180tccccagcga caccagcttg tccccggaaa tattggcggc cagcttcata atcttctcac 240tcacatagta cc 252 79 571 DNA Homo sapiens misc_feature (1)...(71) n = A,T,C or G 79gctcgggcaa gcactttaac cttttaagcc caaccagatg agttgcctgc agttttggag 60gccttcagag catttcacta gacctctgtc tgtgtcggtc cagtgtcttt agccaagctt 120tgattaaaga tgacttcctt gtttgctcaa gaaattcgcc tttctaaaag acatgaagaa 180atagtatcac aaagattaat gttacttcaa caaatggaga ataaattggg tgatcaacac 240acagaaaagg catctcaact ccaaactgtt gagactgctt ttaaaaggaa ccttagtctt 300ttaaaggata tagaagcagc agaaaagtca ctacagacca ggattcaccc acttccacgg 360cctgaggtgg tttctcttga actcgttact gggcatcagt agaagaatat attcccaaat 420ngggacaagt tcttttagga agacccctta tccttttgct ggtgaaaatc aaaatgaagc 480nnaaaatccc ttcaaaatga ggccaacgan taactttttt aaatggcttt tcaaaaagcc 540ntgttaatta ancttnantg taaaggnttt t 571 80 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 80acctcttcct gttcgaatgg gttatccagt aaaaaagggc gtgcccatgg caaaggaggg 60aaatctagaa cttttaaaga ttcccaattt tctgcatttg actcctgtag caattaaaaa 120gcactgtgaa gcccttaaag atttttgcac tgagtggcca gccgcactgg acagtgacga 180gaaatgtgag aagcattttc caattgaaat tgacagcact gattatgttt catcaggacc 240atctgttcgg aaccccagag cacgagtagt agtctcaaga gtaaagcttt ccagtttgaa 300tttagatgat cacgcaaaga agaaattaat taaacttgta ggagagcgat actgcaagac 360cacagatgtg cttaccatca aaacagatag gtgcccttta aggaggcaga attaccatta 420tgccagtgta tctactaaca gtgttatatc atgagtcttg gaatactgaa gaatgggaaa 480aaagttagac tgaagccgac ttggagaatn tatatgggaa aatactatca gaaagaaata 540tctggnaacc cttttccgat gaaagtgctg anaaaatntg gaattaataa gaagn 595 81 601 DNA Homo sapiens misc_feature (1)...(601) n = A,T,C or G 81acgcggggga aaacaagatg gaggattcgg cctcggcctc gctgtcttct gcagccgcta 60ctggaacctc cacctcgact ccagcggccc cgacagcacg gaagcagctg gataaagaac 120aggttagaaa ggcagtggac gctctcttga cgcattgcaa gtccaggaaa aacaattatg 180ggttgctttt gaatgagaat gaaagtttat ttttaatggt ggtattatgg aaaattccaa 240gtaaagaact gagggtcaga ttgaccttgc ctcatagtat tcgatcagat tcagaagata 300tctgtttatt tacgaaggat gaacccaatt caactcctga aaagacagaa cagttttata 360gaaagctttt aaacaagcat ggaattaaaa ccgtttctca gattatctnc cttcaaactc 420taaagaanga atataaatcc tatgaagccc aacttccgnc ttctgagcag ttttgaattc 480ttncttactg atgccagaat tangcngntc ttacccttac tcattgggag acatttctat 540caaagaaaga aagttcagta tctgtaaacc ntttgtccaa gaatttttca ggagagatca 600a 601 82 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 82cgaggtactt tgaatatgga gtagtttaca gctatttttt tttcttactg gtaatcttaa 60ctaatatgat tcccttatta gagagcctct cactccccca cccccaaaaa tgtctactat 120tcatgacagt aaccaattat tctggacaaa ttgcttcttt ttaatttgag ctatctgcca 180tggactttct aaaatggaaa cacagcctga gtgtatctta gggagagttt gattgaaaaa 240atccaaatca ctatccatat agatcatgga tataaagaga tacctgattt ttattaaaaa 300gatacttttt caaatttaag agttaatctt ggaaatttgg aacaagtaaa ggggcaagta 360aaccttttga tgaaatataa aaggactcat tgcatgaagt gactatcaaa ttctgngatg 420tgnggcttct taaaaatatt ctcagggctt tgggggcctg ccanatggta cctgcccggc 480ggccgtcaaa agggcgaatt ccncacactg ggggccgtac tagggggtcc caacttggac 540ccaacctggn gnaaataang gcataantgg tccnggggga aatggtnncc gttccattnc 600cccann 606 83 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 83gcgtggtcgg gccgaggtac acgttcgtca tggcggctgg ccctggacct gggtaggggg 60tccgggttca gtggtaatag cggcggagat gggggagcct ccgcttggct tctttcacac 120gggttgcttc ggaggaatcc gccgtgcaaa tctgtccgcc cccttggcca ctgatccccc 180gaagagcttc tgtcgccgct ctaggaatac agacattgaa gtttgggaca agatatttat 240ctaacttctg tgtcaaaatt agcgacctgc tatggcaatg aagaaagaaa ctgaatttgt 300cattttcacc tgaagaaaaa tgatagacaa aaatcaaacc tgtggtgtag gacaggattc 360tgtgccctat atgatttgct gattcacata ctcgaagaat ggtttggtgt ggaacanttg 420gaggactatt tgaattttgc aaactatctc ttgngggttt tacaccacta atacttttaa 480tacttcctta ctttactatc tttcttctct accttactaa taatttctta cacattatta 540agaagaaaga tgttttgaaa gaagcctact ntcataatta tnggatggtn caagggaaac 600anggcactnt ntg 613 84 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 84ggtactatct gctgctggca aatggggttg ctctgggtga cagggatctg ctgacccaat 60gctatggttt gttccagtca atgagttgag aaggctaaag ccttggttcc tatcattctt 120catcactaca ttggaccaca cattggcatt cagggcttgg acaattcgct ttactcctgt 180agattctggg aagtcatcat cctcctcagg caactcctct ggactaagtt ctaccaattc 240aaagccatgt ttgaggcacc attcttgagc tttttgtcgg tttataccat cttcagacac 300tctatcgcag accaagatca tcacctcagg taaccatgct tttgccagtg gaagccatga 360ggagacacta tcaaggcccg atttttgtgt gctgtcaaag taaaccacaa atgcttggac 420agattctgca atctctgcag taaccagaaa tttgttgggc accccacata gattgagtct 480gctgaaaagt atttattatc aatggncccn ggataaaact acacattatt tggaagtact 540ttcncaataa gaacttntgg tccaaggtat ttttggaccn aanggnctct tgaaaaaacg 600gagga 605 85 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,C or G 85acagggaatg aagactcgaa gaggagatgt cactttcctg gaagatgttt taaatgagat 60tcaattaagg atgctacaga acatggcttc aattaagaca actaaagaac tcaagaaccc 120acaagagact gcagagaggg tcgggctcgc agcactcatt attcaggact tcaaaggttt 180actcttatct gactacaagt tcagctggga tcgtgttttc cagagtcgcg gggacacagg 240tagagtaaac tgcanagctg cctgtctgtg acttccaagg ctaggtcata aaaggagata 300aagcttcttc tggctgggtg ggctgcttgc tcttgaacct tcagtctatg cacgcaacat 360gcctttccag ccttctgtgg ttgtagagtg natagaaagc aattggatca ctatngacag 420cggggtaaaa cttgaggaag caacctccgc caggnggtac atggagganc cctgaannaa 480aggaanaaaa gggcacangg gcttaatcct gtcttggaat gcttncctnt gcaatggnnc 540atttcaatgg ccnagccaat tatgccatcc ctgcnttaan accatgggcc ttcnttgnca 600ttn 603 86 583 DNA Homo sapiens misc_feature (1)...(583) n = A,T,C or G 86actgtaggta tttattaata atagcaatga agatgaaaga gtgatgtatc agagaggtgg 60agataaaatc agtaaaactt agacactaaa tgatagggga aggtggagga gaggaatgag 120cctagaaaac ttagaatata atggttctaa aattaaccaa agtaagggac acaggcatta 180gagtaggttt tgcagagaat gaatgtttta agacacacac aggtgtctct gggacaacca 240agaaaagtgc aacaggcaga tggattgagg agtctggcta aagataagga tttaggaact 300gctgaattaa aattacccaa gcgtgagaag tggtgttgtg attaagagag aaaaaaaaaa 360tggaggtctg aggaatacct ttaanggatt aatgaanang cccaaaggtg ggggggtggt 420caggagtgac ccaaatgtag aagtcaggga ataaacttta aagtnggggt gtcaaaatgc 480naatccgaaa aaaagtnagt nccttggccg gaccccctag gcgaatccac ccctggngcc 540gtctanggat ccacttgncc aacttgggaa nntggctnct ttt 583 87 332 DNA Homo sapiens 87acgcgggggc attgctagaa gccggcagga gtgactctcg gcatggagga cccatctcct 60agcacacgtg cccactgaag tggcaccaac agaagtttgg cttgaactaa aggacatttt 120atttttttta ctttagcaca taatttgtat atttgaaaat aatatatatt attttaccta 180ttagattctg atttgatata caaaggacta agatattttc ttcttgaaga gacttttcga 240ttagtcctca tatatttatc tactaaaata gagtgtttac catgaacagt gtgttgcttc 300agactattac aaagacaact ggggcaggta cc 332 88 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 88cgaggtacgc ggggacaacc agctgactcc cgtagaggaa gacactgtgg aggccagttc 60tggagctatt gcagcctcgg ttgcccggcc cgggacccga acccgaaaaa gttatcgtca 120gaatgtcggg caaagaccga attgaaatct ttccctcgcg aatggcacag accatcatga 180aggctcgttt aaagggagca cagacaggtc gaaacctcct gaagaaaaaa tctgatgcct 240taactcttcg atttcgacag atcctaaaga agatnataga gactaaaatg ttgatgggcc 300aagtgatgag agaagctgcc ttttcactag ctgaagccaa gttcacagca ggtgacttca 360gcactacagg tattccaaat gtcaataaag ccccagtgaa gattcnagcn aagaaagata 420tgtacnagtg gtactttgnc ngtatttgaa cattccntga aggactgcng gttttnactg 480cttgggttaa cccaagtggg gacnnnttgc ttaaattaaa gaggaatttt gcccaancnt 540gggacttctg gnggaattac ttttttggaa actttttggn accttggagn aa 592 89 630 DNA Homo sapiens misc_feature (1)...(630) n = A,T,C or G 89acgcgggggt ctttgggccg gcgcgaacca tggccggcat ggtggacttc caggatgagg 60agcaggtcaa gtcctttttg gagaacatgg aggtggagtg caactaccac tgctaccacg 120agaaggaccc ggacggttgc tatcggctgg tggactattt ggaagggatc cggaagaatt 180ttgatgaggc tgccaaggtg ttgaagttta actgtgaaga gaaccagcac agtgatagct 240gctacaaact gggggcctac tatgtgactg gaaaaggtgg tctgacccaa gacctgaaag 300ctgccccagg tgctttttga tggcgtgtga gaaacctgga aagaaatcaa tagcancatg 360tcacaacgtt ggccttctgg cacatgatgg acagggtaat gaagatggcn acctgacttt 420ggaaaaggca aggactacta ccaaaggcct gngatggngg ntatctttca gtgcttnaaa 480cctaatgcat ttttcttcag ggggcccaag ctttccaagg acatggcctt gcctgtnaat 540cttcattaaa gccttgacct ggtcatattt ggccttgcca tgcaatccat ttacttggcc 600ggacacctan gggaatcacc actggggcgt 630 90 653 DNA Homo sapiens misc_feature (1)...(653) n = A,T,C or G 90ggtaccactt cactccagcc tggcgacaga gtggaactcc gtctcaaaaa ataaaataaa 60ataaaataaa gcaaaaatat aaaatgttaa aaaaaaaaca aaaaaaggga aaaaggaagc 120tgattgcctt ggtgagtcaa cactgggtat tttctgacca ctatttgaaa caaaaaagga 180aaccactgat attctatgca aagatctgtt cctggaaggc actctgcgga gacaccagga 240gaacttttat caatccttca ttgatttgaa gtaaaagtgc taaagcaatg gttggtgggt 300ggcaacccat tagcagatca caaaatcact gtagtgggta actaaacaag aggaaacaca 360agacggcatc ctgtgtaact ggggttaagc attactctct gaaactcatg gcatcagttt 420cctcttaggc tcttcccaca aagtataatc atgttcattt cagtttacaa tcccttgcag 480tcccatcgat ttgtgagaat atcccaagtc atncacagng gagnctggaa atggtcntan 540ttgtcctgcc cggcngccgt tcnaanggcg aattcaacac actggcngcc gttctaatgg 600atccaactcg naccaacctg gnggaacatg gctactggtt ctggngnaaa tgn 653 91 657 DNA Homo sapiens misc_feature (1)...(657) n = A,T,C or G 91actttttttt tttttttttt tttttttttg ggagaaaagc ctnactccgt tgcccacgtt 60ggagtgcagt ggcgtggnca tagcttattg catgcagcct naacctccca ggctnaagca 120atnctccnac ctnnncgtgc tgnnttnntg gaactacnca tncacncnat tatgcccanc 180tngtngttgt naatttaaag tganaccatg cncncaggnn gnatggcntt nnntancnan 240catgcatgct cagctgtgta gtgcacgcac aggataaatg gaagggggat ttgatcaggg 300tttttgtcac atnagcattn naaatccgna ngactgccnt gtgtctgcct ttgnaagggc 360ctgggagtat tctgtgtagc ctttgnaaat aagggnaaaa tgngcncctg ccaaagaagt 420cnttgctact ntgggtgngt caaaatntcc ctgtaacttg tcaatggnca caagcttggn 480ggngtntttg ggntcttggn tgtcnttttn acgtctattg nccatgtggt tcctatatga 540cacantcctc ntnataatcc ntganaattg ctaanntgcn cttttttttt ttttnanatt 600nattttgctn ttaaantagc ttaanncttt ntttatcctn gggcanccna anncaat 657 92 653 DNA Homo sapiens misc_feature (1)...(653) n = A,T,C or G 92accataaaac cattaaaagc aataaataac tagagtcatg tgagatgttt caaagactgc 60tggaggtttc tgtaaaccag ggtaatcaga aatattaccc ttgtagatag ccctctcata 120ccagtaaata caaagagtta aaattccaat gccacagtgt aacagttaac aatctatttt 180gtaattttaa atattactac attaattcac cctgagaata cagaggaaac atttaataca 240agacattctg atatgntttt ttttcccatt gnatttgctt tcttctggnt ttcatcagcc 300ctttaagggc acagatattt taatttaaag ggtgatttgg atatgctttt ttggtaactg 360agatttatgc cacagtcaga tactggtgat agaaaagccc aaaaaggntt gnagaaaaga 420ggcaagcagc aatccccagg cagaaaagac ngaaagtctt gaaaaagaag aggagtaaaa 480atttttttaa gctgntcaat gccctgtatt tgggnacaag tacctttatt ttcctttagc 540tganggnant cagagtaacc gaattggnag nnnactattt tcnctggnaa ggaaaataga 600atttggnaat cccnggaang gtnctngaaa tnnagcccca tccatttgnn gng 653 93 640 DNA Homo sapiens misc_feature (1)...(640) n = A,T,C or G 93acagagaaac cacaggttgc cctttccaca gctggataga cttatccaaa acggcaggat 60ggttctgtat taatcttttt ggaaagcatg tctgtattaa gattgcaaaa catacagata 120gctaccacaa attaggtcaa acgactgatc aagttgtaac atctgtgagg tcaaattcca 180aagtgcctag atacacattt atacaacaga ccataagagc tgaattcttt acaaatgtct 240ttatgggcat gtaaaattga ctctgcattt ctgcatgtgt gcattccata agagagacca 300gtctgcactg agtcatatat actccaactt gaaaaagtaa gtgnaacaac tggntaatca 360tgcaagtctg gttgnaatat aacaatgact ggnaaaacat gaattcttcg cacagtagta 420ataggngcac tnatttaaaa ccctnccgaa aaacctgnat ttggtgcaan atctganttt 480aagnggtagt aacttgacnt ttaaaaatag tttgaacnat ttanaaaggn aagccaactt 540ttacttaaaa gaatcccaag tggnaaaanc tggntttcaa tggaatgaac tnggtgngac 600ctnccctaat nngaccttga gcctatnagc taatntangg 640 94 658 DNA Homo sapiens misc_feature (1)...(658) n = A,T,C or G 94acgcgggcca agcttttttt ttaatttggt gttttctccc atcctttccc tttaaccctc 60agtatcaagc acaaaaattg atggactgat aaaagaacta tcttagaact cagaagaaga 120aagaatcaaa ttcataggat aagtcaatac cttaatggtg gtagagcctt tacctgtagc 180ttgaaagggg aaagattgga ggtaagagag aaaatgaaag aacacctctg ggtccttctg 240tccagttttc aagcactagt cttactcagc tatccattat agttttgccc ttaagaaagt 300catgattaac ttatgaaaaa attatttggg gacaggaatg tgataccttc cttggntttt 360ttttgcaanc ctcaaatcct aacttcctgc cccacaatgg tgagcaggtt cccctgatac 420ttcttttctt taatgattta actatnaact tgnataaata acttataggg gatagggaaa 480attcctgaat tccagaatgc catctgntaa aaaagaatnn aaatgggaag tnggactnaa 540aaggagccaa cagcatgctg cggtggnngn cacttctttg cnctatccca ggaaggaagg 600tccccatttg gaaaggggtt cttnctcact ggnaccggtt tgacntnatt ggnacncc 658 95 392 DNA Homo sapiens misc_feature (1)...(392) n = A,T,C or G 95actcagactt gatcgattaa tgaagtggtt attttggcct ttgcttgata ttatcaactc 60actggtaaca acagtattca tgctcatcgt atctgtgttg gcactgatac cagaaaccac 120aacattgaca gttggtggag gggtgtttgc acttgtgaca gcagtatgct gtcttgccga 180cggggccctt atttaccgga agcttctgtt caatcccagc ggtccttacc agaaaaagcc 240tgtgcatgaa aaaaaaagaa gttttgtaat tttatattac ttnttaagtt tgatactaag 300tattaaacat atttctgnat tcttccaaaa aaaanaaant aatnaantta naanctttta 360aanatanaaa taaaataata angaccattg ag 392 96 655 DNA Homo sapiens misc_feature (1)...(655) n = A,T,C or G 96ggtacaggtt tttatgtgaa catacatttt cattttctgg gataaatgct caaaagggca 60actgttgggt tgtatggtaa acacatatat ttttgtaaga aactacccta ctctttttcc 120agagtggctc tactttttac atacagccac tcatacaatt cagacagcaa tgtatgattg 180atccagtttc ttcacatcct caccagcatt tggtattact actatttttt atcttaacca 240ttcacataga tgtgtgtaat gataccacat gtggttttaa tttgcatttc caatggctaa 300tgatgttgag tatctttttg tgtgctaatt tgccatctat gtatcctctt cggtgaaatg 360tcttcatgtc ttttgnctat tttctattta agncatttgg tctttttact attgagtttg 420agagggtttt tatatatcct agataaaaat cctctggtan anatgtgggt gcctggaatt 480ttaacataac ttctacccan ggaaaataag taaaatttcc acccttgctg gcnagcctta 540cttaatnccg gccttaangg tccttctaga gaattaagaa gatttgaggt ttaaatanaa 600tcagggcntt aaaaagtaat cctaaaatcn ggtttaagca agccatatcc tgggg 655 97 224 DNA Homo sapiens 97acaagtttaa ggtaggacgc agcattttat agtgttacgt ccttcctccc cacattcctg 60tgaggcggaa caagaacaat tacttgaccc tggaggaaga cgacgccttg tggtcaggga 120gagaacagca gttcatgctg gctgcctcgt ctttccaggc ctgctgctgc ccaggcttct 180actgaccttg ttaggtctga ttctagaaaa tgaaggcagg tacc 224 98 582 DNA Homo sapiens misc_feature (1)...(582) n = A,T,C or G 98ggtaccacca tgcctggttt attgttttat ttttttggca gagatgggtc tcactgtgtt 60gcccaggctg atctcaaact cctggcctca agcgatcctc ccatctcagc ctcccaaagt 120gctgggatta cagacctgag ccaccacacc tgggcaacag agtgaaacct gtccctgttt 180tcctgctctt actctcacct ctgaggcctc ctctgcctgg aagagattac agggaaattc 240caggcagccc ttgtcaattg tttttatgaa ttctttacct gttcctttta aagacaagga 300aactgaggcc caaagttcta agttgtttgg caaatggagt ctcctaccct cagctcctgc 360aaggacctgg gggaccccca ggtccagcag ccacatgatt ctgcacagac agggacctag 420agcacatctg gatttaagcc caccctggca actggctgct agagactncc aagatgccga 480taataggatc tgccnttaaa aaatctggat tctggcctgc ntaantgcta cttcatttgg 540ctacaaagnt ttaaggngga accnttaaaa ccttccccaa aa 582 99 619 DNA Homo sapiens misc_feature (1)...(619) n = A,T,C or G 99ggtacagtgg tccttttcag agttggactt ctagactcac ctgttctcac tccctgtttt 60aattcaaccc agccatgcaa tgccaaataa tagaattgct ccctaccagc tgaacaggga 120ggagtctgtg cagtttctga cacttgttgt tgaacatggc taaatacaat gggtatcgct 180gagactaagt tgtagaaatt aacaaatgtg ctgcttggtt aaaatggcta cactcatctg 240actcattctt tattctattt tagttggttt gtatcttgcc taaggtgcgt agtccaactc 300ttggtattac cctcctaata gtcatactag tagtcatact ccctggtgta gtgtattctc 360taaaagcttt aaatgtctgc atgcagccag ccatcaaata gtgaatggtc tctctttggc 420tggaattaca aaactcagag aaaatgtgcc catcangaga acatcataac ccatggaagg 480atnaaagccc caaatggngg naactgataa tagccctaat ggctttaaga atttgggcac 540actnttacct aggngaaccc atttganccn anggggctta aaggcttntt acttcaactg 600aaagttnagg gaaaaaaan 619 100 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 100acgcggggga agcaaaggag agggaagctg gaagcacctt tggcccggga cagaaatctg 60gagagcttgg ctacctccat cctcctcagg ccggagcagg cttcctgaga gagtccaggt 120cgtaggagtt ttacgactta gaaaagcggg ctgcagattc cttcctgggt gtttggttca 180agccctggct ccagcctcac tctcagtctt cccgggagtt cgtgggattt ggaccttaga 240ttattagtat tattttgagg gcctcctgtg tgtaagcact ggttgtgcgc agatggctgt 300gcagagggcc atgaggtaga ggctggggaa atgagggctt ggaggtgctt gaggtatggt 360ctttacctac gtgaaatgtt ggaggttgag atgaaaactc ttgctttgaa atcttcatgg 420aggactacat catttcaatc ctgaatctgg ctcaattcta ttaatcactt aatacctgga 480ttaaaaaacg nttaantggg ccaggcncaa tgggtcacgc ctgnaatccc agccntttgg 540gaggccaagg cangccggat acnttagggc ngnanttnaa accancttgg caaattggga 600aacccgcntt tntn 614 101 625 DNA Homo sapiens misc_feature (1)...(625) n = A,T,C or G 101ggtactttgc ctacggcagc aacctgctga cagagaggat ccacctccga aacccctcgg 60cggcgttctt ctgtgtggcc cgcctgcagg caagaagggg ttaaaagtgg aatgtatgtt 120gtaatagaag ttaaagttgc gactcaagaa ggaaaagaaa taacctgtcg aagttatctg 180atgacaaatt acgaaagtgc tcccnnatcc ccacagtata aaaagattat ttgcatgggt 240gcaaaagaaa atggtttgcc gntggagtat caagagaagt taaaagcaat agaaccaaat 300gactatacag gaaaggtctc agaagaaatt gaaagacatc atcaaaaagg ggnaaacaca 360aactctttag aaccatancn gaatatatct taagggtatt cctatgtgcc taatataata 420tatttttaac acttgagaac cagggatttt gggggattct ccaacgtttg ttcaatttta 480agaantggtt tgaaggagtt ttttacttgg gtnattcntg gttttaggat tttnnanngn 540aanntggntt nggngtttgn nnttttaann gggntntttt ngggtcttna aatttttcca 600anaaanngtn gnttccttcc cggnn 625 102 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 102ggtacaagaa agaaaaaata taaaaacaag tctgctgagt gtcgggagtt ggtgagggat 60atcctaccat attgtgacgg agtccaaata gaaaacatgc agcaacagtt ctcctgcttt 120atcagctccc tggaaaataa accagtaacc ctggtagtgc agtaaccatt tggttaacag 180gacaaacttc ctgatggaca cagatagtaa ttcactgcat ttcccttctc taacttctct 240cttcacacca attccttttc tttcctttaa gatgggtttc atcctgttga caaaagattt 300ggttttattt gtaaagtaaa gcagataata tcctgattga agtattcaat gatttaattg 360aggatgcttg gggatcaaac tttgtaaaaa ggtcaattaa gctagttagc agagactatc 420agtggcttgc agaaaaaaaa ntcngatata tggtttggta aaangcccaa aggataaccg 480ngaaaaatcc tanggatacc gggacctaat taatcaaagc canaggggga ccttggttaa 540anccnttact tnggggangg gctnaanggn ggntccaaac naaattggtt cccaacgggc 600ccggg 605 103 251 DNA Homo sapiens 103acgcgggatt ttacattcca tcttttctga agattgtcct acaatttgga ttttgatcat 60gacaaagaag attaaaattt cattagcatg aatgcaattt gttaaagcag actgatttgt 120ttctaagata tttttggttt ttttaaaact gataataatg ctgaattatc ttaagtgaga 180tgttaagccc actttgttct tttaatgtaa tggagcttat gggtagaaga ccatgtctac 240taattacaaa a 251 104 293 DNA Homo sapiens misc_feature (1)...(293) n = A,T,C or G 104ttaatcttgc acaaatggca ttttattaaa gaaaatctaa tttacaaagc tttgtaaatt 60ttaagaaaaa cattcataga tcataaacaa aaatttcaat atgcaatatt caaatttaca 120agaaaataag cacaaacttt tagacagtgc agttattgct gcactccttt aattccttat 180ccagagccca aaaaatgtag acaaacccta aaaatgtagc agaagcattt ccgcacactg 240gtgtccagaa tctagtttgt gcanaaatgt ttccactaga tttatagagt acc 293 105 586 DNA Homo sapiens misc_feature (1)...(586) n = A,T,C or G 105acatcatttc tgccatgtgg gacattttct tgggaatata caagtaatac tccatgtagc 60ctgacaggtc ctcaatggtc acatcatcca cgaagactcg agcttgctca gaacaggatc 120ggggagagcc agacagagtt ctggcgtgca gcgactgaga gtagtcctca agtgtggatc 180ttcgttctgg agccaaggga gggacactct gcgggcctga aaaggaatac acttccatat 240catgccatct cttacactgg cattccttgc ctatgcatgt gcatggcttg ccctggttta 300gcttggaaac tgattgaaag tcagagagat cactggcttt gagacttgct tgggggactt 360gggtagccgt cagaggagtc ttccttctta ctctctgatg ggagccttgg aacagaaagt 420tctcaaangc tnaacgactg gccctggggt gaatagcatc gagagaagta naccttcttc 480ctgnactgaa ctnttaaggg gatgaaattc ccagccaatg gtggccttan gnnangcaan 540ntggcttttg gcttgaatta ctggntggaa aaaacctttg gccntt 586 106 644 DNA Homo sapiens misc_feature (1)...(644) n = A,T,C or G 106ggtacnttga ttgctcanat ataangaaat ggcccaatga acgtggntgn gggaggggaa 60anangaaaca gagctagnca tatgtgaatt gntctgtgnn ataaacatgt taaaacanac 120aaanatggnt atttttcttt ncctccggac agtgcacatt atcatntgaa ctacctgggg 180attctntatc anaactggtc ttgttgaata tttatactta attgaaataa ttccttanng 240gaggcntgtt taaaacgtat taacaggana ttgtgtntna nacatttaat gaaanacgaa 300attccacnag aatganntaa gtcactttcc aagtgggtgt cattttgtta aaccctngtt 360tacctgtttt gctattntta ccntttcatt tggaangatg ntttgagntc gtanttacca 420gggnaaagac gggttncttc ctngctgnnn cttnagccnn tgctaaaaag cnttaatttt 480ntgcnattng gnncttcctg ctggtaatcn tggaaaaant gggnnaantc cagctttntt 540tnttggcngc ccaaaaangg attcnnantn gnnannnaac ctttggttcc ntaannaana 600aaangtatnc anaangaacc ttgncatgcc ngccnntnta aang 644 107 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 107ggtacagact tgccctttga aatctatacc tctggataca ttagaggcat tttattaaca 60aaggcccttc taaatgtgct atttatttga caataactat cagatttgcc ttaattttgt 120gtttatagca tttatcaaaa cgtatcctca tagactttat gcagattaat atggtcaatt 180gatttggata aaagaaagta atttcagggt ttgtttttaa gccaggacaa gaagtgcaaa 240tgcctctttg aagcaattta ggctaaactg attttgaaat ttcaaaatgt tttattttac 300tttgttttat taagccagga caagaagtgc aaatgccctc ttttgaagca attcaggcta 360ggtaaacccg attttggcca tttcaaaacc gtttaattta ctttggttta atatcagagt 420cttataaaac tgntgncaaa aatttctgaa ggctttngaa aagggttggt agtggaccct 480gcccgggcgg ccgntcnaag gcgaattcag ccactggcgg ncgtactagg gatnccactc 540ggacccanct tggcggaatc atgggcataa ctggttcctg ngtgaaatgg gatccgttac 600aattcccaca acatanng 618 108 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 108ggtaccaaag gagaatttgg agagctggct aaattatttg aagaaagaat tgccaacagt 60ggtgttcaga gcctcaacaa aaccaaagga taaagggaag ataaccaagc gtgtgaaggc 120aaagaagaat gctgctccat tcagaagtga agtctgcttt gggaaagagg gcctttggaa 180acttcttgga ggttttcagg aaacttgcag caaagccatt cgggttggag taattggttt 240cccaaatgtg gggaaaagca gcattatcaa tagcttaaaa caagaacaga tgtgtaatgt 300tggtgtatcc atggggctta caaggagcat gcaagttgtc ccctttggac aaacagatca 360caatcataga tagccccgac cttcatcgaa tctncactta attccttctt tgngccttgn 420ttttgcnaag ttcanccaag gttttgaagt antaaaancc gatggaagct tgccantgcc 480atcctttcca agcttgatgc ttgacaggta gtancttgnc cgggccggcc gttcnaaagg 540gcgaattcaa cacactggcn gccgtactat ggatccgagc ttggnccaaa cttgcgtaat 600catggcatnc tggttcctgg 620 109 317 DNA Homo sapiens misc_feature (1)...(317) n = A,T,C or G 109tttgtatttt tagtagaggc agtgtttcac cgtgttagcc aggatggtct cgatctcctg 60acctcgtgat ccacccacct cgacctccca aagtgctggg attacaggcg tgagccacca 120cgcccggcct cttttttttt tagctgccaa tctttttgaa ggaatattct tacctctact 180ttgtcacctt ctactggctc cttaactaaa atctgccatt tggctctctg gttaacagtc 240ccttcctgta aagtctaaaa tcttaattct aaatccacag tttaattcac aagctagtac 300cttggccgng accacgc 317 110 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,C or G 110ggtacattca ggatccctcg gccaaggact ggaccagaag aacacttggg aatcttgggt 60ccacttatca aaggtgaagt tggtgatatc ctgactgtgg tattcaagaa taatgccagc 120cgcccctact ctgtgcatgc tcatggagtg ctagaatcta ctactgtctg gccactggct 180gctgagcctg gtgaggtggt cacttatcag tggaacatcc cagagaggtc tggccctggg 240cccaatgact ctgcttgtgt ttcctggatc tattattctg cagtggatcc catcaaggac 300atgtatagtg gcctggtggg gcccttggct atctgccaaa agggcatcct ggaaccccat 360ggaagaccga gtgacctgga tcnggaattt gcattggtgg tcctgaattt tgatgaaaat 420aancctggna tttggaagga aatgtgcaac catgggtcca agaatccagc cnnattaacc 480taccggatga acctttnttg gaaaccataa aatgcctgca atcaatggga actttttcca 540accttanggg cttaccatga ccttgcccgg ccggccnttt aaanggccaa ttccaccccc 600tgg 603 111 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 111acatttaagt tcccatgtta cagaatccca tattgtgact atttcctcaa aactaactgc 60tagtaaagaa ccatcttcgg agaaacaaca gttagttgct tgatacttgt gataactacc 120aacaaagtca caggtccagc caacagcttt tttgtatatg tcagagtcat ctgttaatat 180ccatactttg aagtaaccat ctttgctagc tgtaaccaag gtgggctgtt cagatttttc 240tgcattacag aaacagagag ctgtaatgca gtcttcgtgt ggcatgttaa ttttagtgtt 300aagaataaac ccttgtgttt tcttattata catccacagt ttcatttgca attcaagctc 360aagtttcctt ttcttgccgc tggtccactg gtgcaagcca gttaccaaag cagccaatgc 420aagccttggt aagtcaattt ggatcaganc ataatcanta atatatcctg ctggataata 480ctaaattgga tactggntat cactntggag agaataaact gcaggtggcn ggntttcatt 540caaaccaagc tttagtcttg gacaatcatn aaccagngaa atactcctat ntttn 595 112 523 DNA Homo sapiens misc_feature (1)...(523) n = A,T,C or G 112acaagagcta ttagagatgc tgccatatgg atgggcaaaa ctgagccaat cccacttagg 60aatggaaggc ttggacatgg aagggaggat ataaacgagg agttggagaa aaacgcaagc 120ccagtttttg ctagagtgga aatgaaagtg ggaatgaggg tcttgttttt agtcctctaa 180ggaccaggaa gcaattttaa aacttccttg gtttttctga aagcagcata ttcaaaatgc 240cagcaaaaac tcctaacaac tgcaaaacca aaagaggatc aaagctcacc aacatccctt 300cttattgctg aaaggctcta aaattcagga tgccctgttc ccttgtaaaa gggaaaataa 360ttaaagtctg atttatggta atcataccac atcacacttc taaaaaaata tttcaagtgt 420gtgaccaggg gaccgtttga ccnccatttt attaaccttc actttantgg gaaaaataaa 480accttttcca gggccatttn atnccaggac ttttagtagg ggg 523 113 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 113acagtgtaaa taactaagtt gttaactgtc aagtccagtt atgtattctg taagttgtgt 60tctagtcttt gactaaaatt tatcatctct tataatggga cttaatcttt ctctaaaagc 120atataagagc ttgtcaatag agcaatcaat caaaaagatt ttgtgattca taacattgaa 180gttagtctgg ttaagagttt tggtttagac ttcatttata ttttccttac taatatctaa 240tatttaatga ataatgatca attttttata aagttattaa tatgatcagg gaaacctttg 300ggacttctga caggcatctg gtgaagagac aattcaagcc ttagtgacta tttagaatag 360ccagtgatca ctagctaatt ctcatatcca tgcctttttt gccctggtta cagtcttaaa 420agaggtaaaa cagcaaatat tttttttaag ggaactataa ccctangaat tcctgaaaag 480aatttcaaaa aaaataagac cctgtggcca tggngnccaa acntaagacc tactatggct 540atattggtcc attaaaaata aattactact aatccaaa 578 114 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 114acggtagtaa gaaacctttg agatctttct gacttttcaa aattagagaa agcaaatggg 60atggatagat tttttttttc ttttcaaggg gggcaggaag gtaatggttt gagtagcctt 120tgtttaaaaa aaaactaaat atatttaaaa ggccacattt atattttttt cacaagaacc 180acataataaa ttccacttct tgacctgaat ttggaaatcc gaaattacta atccaggcca 240ggtgtggtgg ctcatgcctg taatcccagc actttgagag gccgaggtgg gcagatcact 300tgaggcctgg agttcaagac caccttggcg aacacggtga aaccccgtct ctacgaaaaa 360aaaaanatat aaaaaaagta ctggttatta accaaccagc ttagaaaaat aatcatggtn 420gacacnttan ttcattcttc taaaagcctg ttgatctggg ccttcctgtt gccagcattt 480cccctttttc aaaaatgggg ggccttttct ttaattnnac ctcgtggngn aananaattt 540gaagggcccc aggaagttnt ttgggcnctt tgaagcgttt tncacncgtn tagattctnt 600gattaaatcc tcc 613 115 190 DNA Homo sapiens 115ggtacattgc cactgagtaa agagtggcac cagccacggt ggtaggtgga agaaacatag 60atcccaatga ggacacaaag acgagaccca ggcccactcc caggggtgca cccatgttca 120gaaacttttc actgggcgca cacatggcca cagtggagag gcctcccaca atgccagctg 180tgtacttttt 190 116 610 DNA Homo sapiens misc_feature (1)...(610) n = A,T,C or G 116ggtactcttg gtttatcaat gggacgttcc agcaatccac acaagagctc tttatcccca 60acatcactgt gaataatagc ggatcctata tgtgccaagc ccataactca gccactggcc 120tcaataggac cacagtcacg atgatcacag tctctggaag tgctcctgtc ctctcagctg 180tggccaccgt cggcatcacg attggagtgc tggccagggt ggctctgata tagcagccct 240ggtgtatttt cgatatttca ggaagactgg cagattggac cagaccctga attcttctag 300ctcctncaat cccattttat cccatggaac cactaaaaac aaggtctgct ctgctcctga 360gccctatatg ctggagatgg acaactcaat gaaaatttaa agggaaaacc cttangcctg 420aaggtgtgtg ccacttcaga gactttacct taacttgaga cngntcaaac ttgcaaacca 480tggngnggaa atttgccgaa ctttacactt tgggcaggtt ttttcccaga agtcanaaca 540agaactcctn ntcttganaa gggttttanc ccctttnaat ggccttgctt atgctgcctt 600tttcgtttgg 610 117 608 DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 117ggtacgcggg gggtattatt tgtgccaacc aatgatgctt ttaagggaat gactagtgaa 60gaaaaagaaa ttctgatacg ggacaaaaat gctcttcaaa acatcattct ttatcacctg 120acaccaggag ttttcattgg aaaaggattt gaacctggtg ttactaacat tttaaagacc 180acacaaggaa gcaaaatctt tctgaaagaa gtaaatgata cacttctggt gaatgaattg 240aaatcaaaag aatctgacat catgacaaca aatggtgtaa ttcatgttgt agataaactc 300ctctatccag cagacacacc tgttggaaat gatcaactgc tggaaatact taataaatta 360atcaaatcat ccaaattaag tttgttcgtg gtagcacctt caaagaaaat ccccgtgact 420gctatagacc cacactaacc aaaggtcaaa attgaaaggt gacctgaatt cagactggat 480taaagaaagg tgaaaccatt actgaaagtg gatncatggg gaagccattt tttaaaaaat 540nccccaaanc attgatggga attccttnng gaaatacttg aaaggaaccn nnnnagacca 600atcnttcc 608 118 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 118actccactta gcaaatgccc tgccagcaaa gtcacagatg acttttttac ccaatcttag 60gtaaatctgg attatctgcc caaccgtgca agtcaataag ccacccttga aaactgtgtc 120aagatttgag gaaacaggtc ttaagaacct atccaacaca tgattccata accaatacat 180cttangttgt tttaggcaaa taggtgtatc tcttgaatca ctgatggatt caatatcaag 240atctataatt ttcacgttta aaatttactc tgccgaggac attttattgg taaagcataa 300accagttagt ttgacagaca cnaaaaagaa aacnaaatgt tcacagtcct atcttcgtag 360ggattcttgg ctataaaaat tggcttcagg ttcaaggtct tagaccactc ttctaaggct 420nctactggat atantantta ccacttgggg nccaaactta aaacctcntg gactttttcc 480ccttanggac nangaaaaac caaggggttg tggtttgaac tccntacact tggngnnaaa 540ncttttcttg gnngnatnta aanattaagg ggcttttn 578 119 584 DNA Homo sapiens misc_feature (1)...(584) n = A,T,C or G 119actgtcttag aatattattt atttttttgt atttgtaaat ctgtggacaa aagagggttt 60cctcactcct tttactcact gggctcatga cagtgaagga gatgctccat ctgcttctcc 120ccctttctct tgctgtagtc caatgtgcta tgagcatcag cttactttgc cacttagagc 180aagcaaaacc cagtgcaaga gtctcgttca gctctaaata ggtttgcttt cttttagtta 240cagtgcccat tttgaaattg cctatacagt cttagtgacc atttaaaccg gacgaactan 300gcgtttaatt ttcacttctt catgttnaat tngcagttca anatttatag naagatggnt 360atttcgaaaa nacaaaaaan tggnttttta anaaaanaag tncnttggtc ggcgaancan 420gcntaagggg cgaatttcca gcncaactgg gcnggcccgt nncntagngg atccccaacc 480tttggtaccc angcttnggc nntaancaat tggnccanag nttgtttccc tggggtgaaa 540antngtnatc ccgttcccaa ttcccnnaca ncnnaccnng cccg 584 120 587 DNA Homo sapiens misc_feature (1)...(587) n = A,T,C or G 120acgcgggggc cgtagcagcc gccgcccatc cctctttgtg tgctttggaa agccgcggag 60ctggtggtgg ctacagttgg tgttgggggc ttaggcgagg gacgttaccg ggaagttgca 120ggcgggagga ctcttcccca tccagtcacc tgacaggtca caaacatgtc agacaaaagt 180gaattaaagg ctgagttgga acgtaagaag cagcgactgg cccaaatcag agaggaaaag 240aagagaaaag aagangaagg gaaaaaaaaa gaaacagacc anaataagga agctgttgct 300cctgtgcaag aagaatcaga tctttgaaaa aaaaaggaga gaagctnaaa gcatttgctt 360caaagcatgg ggctaacttc agaaatcccc ccattggncc ttcctnctaa tncttncatn 420ccttcaaaat ctgtggagcc ctttccaagg tgaaacttgn aannccaaga antntggaaa 480atggcnccct tggggaatct agaccnaggg ncctttttna accttggaat ngnttaaaaa 540tcacnccaag nttgactttt ccttccttcg anaaaattgg gtcccnn 587 121 570 DNA Homo sapiens misc_feature (1)...(570) n = A,T,C or G 121ggtactcttg gtttatcaat gggacgttcc agcaatccac acaagagctc tttatcccca 60acatcactgt gaataatagc ggatcctata tgtgccaagc ccataactca gccactggcc 120tcaataggac cacagtcacg atgatcacag tctctggaag tgctcctgtc ctctcagctg 180tggccaccgt cggcatcacg attggagtgc tggccagggt ggctctgata tagcagccct 240ggtgtatttt cgatatttca ggaagactgg cagattggac cagaccctga attcttctag 300ctcctncaat cccattttat cccatggaac cactaanaac aaggtctgct ctgcttctga 360agncctatat gctggagatg gacaacttaa tgaaanattt aaanggggaa aacccttaag 420ccttgaggtg tgtgnccact tcanaggact ttaaccttaa ctttgagacc aggtcaacct 480ggnaanccct tggtggagaa attggccgaa cttcccnact ttggccaggn ttttcccang 540antgtcaaan caagacttcc ttatcatgnn 570 122 551 DNA Homo sapiens misc_feature (1)...(551) n = A,T,C or G 122actatctcta ttcaggatta tgaagttttt cgatgcgaag attcactgga tgaaagaaag 60ataaaagggg tcattgagct caggaagagc ttactgtctg ccttgagaac ttatgaacca 120tatggatccc tggttcaaca aatacgaatt ctgctgctgg gtccaattgg agctgggaag 180tccagctttt tcaactcagt gaggtctgtt ttccaagggc atgtaacgca tcaggctttg 240gtgggcacta atacaactgg gatatctgag aagtatagga catactctat tagagacggn 300aaagatggca aatacctgcc cgtttattct gtgtgactca ctggggctga gtgagaaaga 360aggcggnctg tgcagggatg acatattcta tatctttgac ggtaaccatt cgtgatagat 420nccagtttaa ttcccatgga atcaaatcaa attaaatcat catgactacc ttggttcccc 480atcggttgaa gggacngnat tcattggggn ggcattggat ttgatnncna gntttattca 540atactttctc n 551 123 575 DNA Homo sapiens misc_feature (1)...(575) n = A,T,C or G 123acttaataca tattttcaaa cctgtttgca tttcaaacaa agttagcgtt tttgtaaatc 60aaatttgata acccgactaa aaatattttc cagctttatt atttaaggag ctgcacagcc 120tttaaagtgg ggaccaggag gcaggcagag gcagagagac tgaatgcacc caggactgcg 180cagcagtcta cagcaacatg tcccacaact ttggtgctgg aaacacaagt aatgcacaag 240acagctgccc tccagtgtca ggatcctgtg aaacagcata tcaaaagatc gccagcttct 300tataatttac acactttcat ttaggattgc ttttttgaag aaaaatcttt aagaatgcca 360tttttaattt aatatccaga accctggaat ttaaaaaaac ctaatngaaa ggaaattaac 420tggtaccatc aaaaatgggg ntgntggttg gancntgtgt gaagttaggg aattctatgg 480ctttttttaa gatgccccgg aaaatttaac cccttaatng cangtttaat ttngaattcn 540cnccaggtan tgtatgtnng gctcanatta gtanc 575 124 570 DNA Homo sapiens misc_feature (1)...(570) n = A,T,C or G 124actgagacaa tggttagggt tgttttctta attcttttcc tggtagggca acaagaacca 60tttccaatct agaggaaagc tccccagcat tgcttgctcc tgggcaaaca ttgctcttga 120gttaagtgac ctaattcccc tgggagacat acgcatcaac tgtggaggtc cgaggggatg 180agaagggata cccaccacct ttcaagggtc acaagctcac tctctgacaa gtcataatag 240ggacactgct tctatccctc caatggagag attctggnaa cctttgaaca gcccagagct 300tgcaanctag ccttacccaa aangactgga aangagacat atctntcaag cttttttcag 360gaangcgtnc ctgggaatcc aaggaacttt ttgatgctaa ttanaaangc ttgggactta 420aaaatgtccn ctanggngtg gcacttttac angtttttgg aangcttnga aggcaganng 480gggtcnaana ntnaaaanac nnttgacntg ntaatanngg aatantangg cnaatggaaa 540ctgngttggg ggaggatcaa tttaaagagg 570 125 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 125ggtacagaga tttaaatgaa atcttcgaaa gaataaattt gcttttcagt ccactgtatt 60ttcaaaattg attatcacca agcttggatg aaagctgtga accacaaacc atttgtttat 120ttaatagaaa aaagaatgtg tagattatta gcaaagtaat gccttaaaat gtatcttcac 180acagttgaaa ttttagtata aacttgtata tcaagttgct ttccattatt tattctactt 240taaaaatata tacaactatg atgttcaaat atgtattctg agccattatg ttcaaacata 300aatatctggg aaattcaaac tgctgcaaca agttaggaaa ggattaagga aaaatgatga 360gctacaaatt atgtagttgg aggaagaaaa aaatgttact tagcatttat gtctggatag 420gtatgtattt tctaatttac atacacatat ccagttgagt atagaccacc atcaaaatgt 480accagttaca cagagactag actaaaccac cctatttcta tacaggtacc atagtggatt 540caaaaattta atatctcata gttcccaaaa ttattgnggn aatatgctna ttt 593 126 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 126acgcgggggg gccttccggg acgagggcgc gtgggtgagg aaggtcaggt ctaggaactc 60taactccttg ccactcaaga aatgtcctcc ctttcagaat atgccttccg catgtctcgt 120ctcagtgccc ggctatttgg tgaagtcacc aggcctacta attccaagtc tatgaaagtg 180gtgaaactgt ttagtgaact gcccttggcc aagaagaagg agacttatga ttggtatcca 240aatcaccaca cttacgctga actcatgcag acgctccgat ttcttggact ctacagagat 300gagcatcagg attttatgga tgagcaaaaa cgactaaaga agcttcgtgg aaaggagaaa 360ccaaagaaag gagaagggaa aagagcagca aaaaggaaat agtgttggtc ccttcaagag 420ggagactttc ttcctaatgg ccggaaagaa gaaagtgcat ttattggctt tccacatatt 480ggaggaatgt catcttccta aatgaagttt atttggagga acacagtcat ttccttggtg 540aaactaatcc ggtacattgn ggttgggttt ttgaacacat ctactgggca aa 592 127 600 DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 127acagtggtcc ttttcagagt tggacttcta gactcacctg ttctcactcc ctgttttaat 60tcaacccagc catgcaatgc caaataatag aattgctccc taccagctga acagggagga 120gtctgtgcag tttctgacac ttgttgttga acatggctaa atacaatggg tatcgctgag 180actaagttgt agaaattaac aaatgtgctg cttggttaaa atggctacac tcatctgact 240cattctttat tctattttag ttggtttgta tcttgcctaa ggtgcgtagt ccaactcttg 300gtattaccct cctaatagtc atactagtag tcatactccc tggtgtagtg tattctctaa 360aaagctttaa atgtctgcat tgcanccagc catcaaatag tgaatgggct ctcttttggc 420ntggaattcc aaaacntcag agaaatggtg tcatcaagga gaaccttcat aaccccntga 480anggattaaa aagccccaaa tggggggaac tgataatagc acttaaggct ttaagaattg 540gncacanttt caccttgtga acccattnna cnatngngcc taanngctnc ctnctncaan 600 128 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 128ggtacttttt tttttttttt tttttttttt tttttttgag acggagtctc actctgtcac 60ccaggctgga gtgcagtggc atgatcttgg ctcactgcaa gctctgcctc ctgggttcac 120gccattctcc tgcctcagcc tcctgagtag ctgggactac aggcgtccgc caccacgccc 180agctaatttt ttgtattttt ggtananaca gggtttcacc gngttagcca ggatggnctc 240catctcctga cctcgtgatc tgcccacctn ggccttccaa agtgctggga ttacaggcat 300gagccacggc gcctggccag gatggtatat ttttaactcc ttcactgggc cccacccctg 360actttctgct ttangaggtc tggggtgagg ctgaanatct gggggccaca cttcgagagc 420aaccaagact gtaagtgggg ccttccanag cccaatgaag ggaatactta ggtacaggan 480gtgtctgcat ggncncangt gtggggtttn cttctcggcc ttaaccagaa agtatctctg 540gttttaattt taaaatgaaa attttaaagg gtgnctgaaa cnaattgg 588 129 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 129ggtactgccc tctccagatc agcagttcag gagagcacag gaggcaaaac acagattgct 60gggcttattg gtgccatcat cgtgctgatg gtcgttctag ccattggatt tctcctggcg 120cctctacaaa agtccgtcct ggcagcttta gcattgggaa acttaaaggg aatgctgatg 180cagtttgctg aaataggcag attgtggcga aaggacaaat atgattgttt aatttggatc 240atgaccttca tcttcaccat tgtcctggga ctcgggttag gcctggcagc tagtgtggca 300tttcaactgc taaccatcgt gttcaggacc caatttccaa aatgcagcac gctggctaat 360attggaagaa ccaacatcta taagaataaa aaagattatt atgatatgta tgagccagaa 420ggagtgaaaa ttttcagatg tccatctcct atctactttg caaacattgg tttctttagg 480cggaacttat cgatgctgnt ggctttagtc ccttcgaatt tacgcaagcg cacaaacttt 540gaggaaaatc cgaaactgcn aagcaagntt gntacaagtg acccaaan 588 130 190 DNA Homo sapiens 130ggtacaaaaa aaaccttaca taaattaaga atgaatacat ttacaggcgt aaatgcaaac 60cgcttccaat tcaaagcaag taacagccca cggtgttctg gccaaagaca tcagctaaga 120aaggaaactg ggtcctacgg cttggacttt ccaaccctga cagacccgca agaccccgcg 180tacttttttt 190 131 386 DNA Homo sapiens 131ggtacagaac tcagaggaaa aaagaaatta aattttagct ttctggagag cagcccctct 60ctggcaccat caaacacttc tttgtttccc ttcaacttgg aactcttcaa acatcagggg 120ttgtgagggt ttggccattc ttttatcttg ggtccatgtg agtgacagaa atggtgcggc 180ctgggaaaga tctccctcct ttacattttc tcttctccct cctcctcctt attctaaaac 240tgtgcctcca acagaggggc aggggctctt gtagagagat ccctggccca ggacaggaga 300tgccaaatct aatttatctc actgagggcc tttgagaaaa acgcttcagg gccaggctca 360gtggctcatg cctatataat cccagt 386 132 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 132actgagacaa tggttagggt tgttttctta attcttttcc tggtagggca acaagaacca 60tttccaatct agaggaaagc tccccagcat tgcttgctcc tgggcaaaca ttgctcttga 120gttaagtgac ctaattcccc tgggagacat acgcatcaac tgtggaggtc cgaggggatg 180agaagggata cccaccacct ttcaagggtc acaagctcac tctctgacaa gtcagaatag 240ggacactgct tctatccctc caatggagag attctggcaa cctttgaaca gcccagagct 300tgcaacctag cctcacccaa gaagactgga aagagacata tctctcagct ttttcaggag 360gcgtgcctgg gaatccagga actttttgat gctaattaga aggcctggac taaaaatgtc 420actatngggt gcactctaca gtttttgaaa tgctaggang cagaagggca aaaataaaaa 480acatgacctg gttgaaggaa naaaagcaaa gaaacttggg ngggaggaca attaaaaaga 540gnncctggga tcccctnttc ttaggtccct ctcttacnaa ggacnctntt tat 593 133 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 133acagancatt nnnagcnctn gcacaggnta cagaacctna cagacccaaa ggaacatcgg 60ataggcnaag cgactacagg aggcgtgtgt gcgcttgggc naggtaaaca gggtcagtat 120tggtcnngtg acaagagnca cgaantctgg ccngacantg angtnaanaa ggttnatnnt 180ttnacantta tnnnanatat nnnnnaannt attaanctgc ancanntgat tttnacacct 240anttactaga aaactaanga aagcactnat tagctctgaa tnaantnaca tggnaagcct 300tttactaatc tncaaanaaa ccttctctgc antatnnnaa agattttatn atacaangng 360gnnnatcnct cnatcatann gggttctatt ananaaccct gctaantntg cgacttacag 420aacanccagc ntananatga ntttcatgcc catttgggaa gcatngcccg ggtatcacaa 480aggaaaccta ctaaagnttt ctgttatacc agccttcntt cntatcantg catgngnana 540nanaaccntt gaaggttntc cnggggactt tnttctnttn ctttgccc 588 134 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 134tcnagcggcc nnccnggcag gtacantcac annttnnang anctnaacac anactanctg 60nngtcaaata ttnaacaaaa gcantagatg aanctgctta acattcacgg aaaaacaacc 120aaaagaaggg aggggtgata aaccanaaaa atgantgacn aaaactaaga gacctcatan 180gngtctttac aatcnggaat tcagatgcaa ggaacagacn caaanctgtc taaaatgtna 240cctatgaggc nacanaaagt gacttaaagt ctggtntnan taaaaaatga caacccttat 300cctagagagt cttacnttat ttaatccana cnttatntaa cgccncngat ttttgnttgg 360ngctatggng ttnattttnt atcagaanga antgtgggac anatgcatta ctgnttgttn 420aaagngcttn acagctaatt cacncccnng ggcatggtca aaaaggnaan aaccnggnca 480tatattgntg anatgaaaaa accacntgtt aaaaaaataa ntgnagccna ntgngttttn 540natgataacc aaatnttnac nttcagtann ngccttttan aagttggtga actccgaaat 600ctnctttttt aaaccngg 618 135 374 DNA Homo sapiens misc_feature (1)...(374) n = A,T,C or G 135actttttttt tttttttttt tttttttttg gggatggagt ctcactctgt tgtccaggtt 60ggagtgcagt ggtgtgatct cggctcactg caacctntgc ctcccaagtg attctcctgg 120ctcancctcc tgagtagctg ggactacagg catgcactac catgcccggc taatttttgt 180atttttagta nanacagggt ttcaccatgt tggccaggct ggtcttgatc tcctaatctc 240aggtgatccg cctgcctcan cctcctaaag tgctgggatt acaggcatga gccactgtgt 300ntggccaana ncactcgtaa gaaggatggc agtatcacaa aatcaagcca gagatacaga 360gattacccgc gtcc 374 136 581 DNA Homo sapiens misc_feature (1)...(581) n = A,T,C or G 136actccagcct tgctgaagct gcctcaaagg ctgatggttt ggcagttatt ggtgttttga 60tgaaggttgg tgaggccaac ccaaagctgc agaaagtact tgatgccctc caagcaatta 120aaaccaaggg caaacgagcc ccattcacaa attttgaccc ctctactctc cttccttcat 180ccctggattt ctggacctac cctggctctc tgactcatcc tcctctttat gagagtgtaa 240cttggatcat ctgtaaggag agcatcagtg tcagctcaga gcagctggca caattccgca 300gccttctatc aaatgttgaa ggtgataacg ctgtccccat gcagcacaac aaccgccaac 360ccaacctctg aagggcagaa caagtgagag cttcattttg atgattctga gaagaaactt 420gtncttctca agaacacaac cctgcttctg acataatnca ataaaataat aattttaaaa 480aataaattat ttcaatatta ncaagacaca tgccttnaat natctgtaaa ctaaaaacta 540aaatttantc tactgnttaa tcnaanataa taatagcttc a 581 137 504 DNA Homo sapiens misc_feature (1)...(504) n = A,T,C or G 137ttttncaaan nnaagttttt tacttccnaa aantnatggc taaggggngg gnggngggng 60aaaaaagnaa aacaaaaaaa ccccaaaaaa atggggnggn naaaaggggg gganaaaaaa 120ccnntntttt ntaaantntn acaaggcaag ngcnnangga aaaaaaaaan ncctgnaaaa 180tcccccncgg nnggggnaaa natnnnggtt tccttttgnt ttnaaacccn ntnangnaag 240gntntccccc ntncccctna atnaaaaatt tntntnccng ggccnnaacc nccntanggg 300naaattccac cncnctgggg gccgttanta agggatccna gctnggccca ancttggnga 360aacatggcaa aactgttcct nnggnaaaat gtttcccctc anaattccca naaaataaaa 420ccggaacata aagngaaaac cngggggcct aagngggncn cacnccattt attggggtgg 480cccncgnccc tttcaaangg aaac 504 138 386 DNA Homo sapiens 138acaacaaata acactgtgac tccaacctca caacctgtgc gaaagtctac ctttgatgca 60gccagtttca ttggaggaat tgtcctggtc ttgggtgtgc aggctgtaat tttctttctt 120tataaattct gcaaatctaa agaacgaaat taccacactc tgtaaacaga cccattgaat 180taataaggac tggtgattca tttgtgtaac tcactgaagc caaaatacta tcttttaaga 240tgtcccacat ggaagacgct attccaggat ctttaaattt ccatggatgc atataggatg 300tttgggagca tcatccgtga agaaaaaatc aattaaatca ttgtgttcaa caggaatatt 360taaaataaaa aaaaaaaaaa agtacc 386 139 586 DNA Homo sapiens misc_feature (1)...(586) n = A,T,C or G 139ggtactcaag tttataatgt ccccaaacct taagactaga aaatcatccc aagaaaaagg 60cctatagttg gtttaatttc accctgagaa tactgtgata aaaatcaata tatttcagag 120ctagtaagta tttaaaaatt agtgtctcaa aaaggggaca tcataaggga aatacagggt 180ttagaggtct gagctcaagt ggtgtaagac agttctttct tcttcctcct ttaaactctt 240cactttgctc taacacggaa gatgggggac agtgatcccg aaggtattac taaaatattg 300cagctttcag taattatgag aagcacagat atcaccagaa aagaaagcaa tcatttggag 360tactaagaaa cgaaacaatg ttatttggtg gtgtataatt ctacttttct agtagattac 420tgngtggaat tctgtgaaaa atatttgaga aaangcctgt attgcataaa taaatctttg 480tatgttgcaa aaaaaaaaaa aaaaaaaagt acctgccggc cgncccaang gcgaattcca 540cacctgcggc cgtctagngg tccacccggt ccacttgggt atatgg 586 140 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 140acagggagga atttgaagta gatagaaacc gacctggatt actccggtct gaactcagat 60cacgtaggac tttaatcgtt gaacaaacga acctttaata gcggctgcac catcgggatg 120tccctgnacc aaccttcaag gccnaaaccc nnntggtgnn tttggnctnt aaatnaggat 180ggccctgtnt tccntaggta acttgttccg ttggtcaagt tattggatca attgagtata 240gtagttcgct ttgactggtg aagtcttnac cnngtccntt tngngtgggg tttttttagg 300naaaagnctt ttggtncatt nntggggggg gnaggggact gaacctttat tntttccaaa 360tncaccttaa antcagggac aanaaacatt ccaanaacca caatctttta aaaaattaac 420tngccagtgg gaatgtttaa aaanntnaan ggtctttttt gccttggttt ttgtgggggt 480ctctcttccc ccccctgggg ttaatttttn aagccgggac ctcncnaana cccctttttt 540caaagggccc naaacccccc cccccnaaaa aaaaaaaaaa aaaaaaaanc n 591 141 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 141ggtacacaaa ccaagacaat atcagggtga caggtgaatg aacttaaatt ctcagtcttg 60tctattcacc aaaaaagtat actgcctgtt ttttctttaa ttattcaagg ttgatgactt 120ttaggaacat gttttatact gtatttttta attaaagcaa gtgccttgat gtaattccat 180gtaaatcatt gcttaaccct cttatgggat gaggatgagt tattaatgta ttgcagccta 240ctggaaagga gggggagttg gttaatagca gatacttttc ttctagaagc ttatgtttta 300tgctgtttat tatgtaagat cctgtatgtg tgttgagatt tagaggtttc atttgttttg 360tctgctaata aattgttact ctaataataa ccnngnnaaa naaannnnnn nnnnnnnnnn 420nnnannnggt ncctgcccng gcggccgctc gaaagggcga attccancca ctggcnggcg 480gtactaaggg gatccgnctc gggncccaac ttggcgtaat atnggcatac tggttcccgg 540gngaaatggt atncgtcaaa ttccccaaat acnaccggaa ncttaagggt aa 592 142 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 142acaacacctt cattcttaat gcttcttagg gcatcacagg ttttagaaat taatgtattt 60ttagcattcc acagtaatga tcactttcaa aaactgcaat atacatctgc atgttacact 120gacatacaac acataagtat tttgtcacac atcaactttt agcctcaaat aatagaatac 180aaaaagctac actggacata acaccaccga acttttgaat atcccctttt cccaattgtt 240aacaggtagt actgggatta caggcgtgag cctctgcgcc tggccaagtg gaggttatta 300ttaaccctat ttaacagata taaaaagaag agattagaga attttatcaa tgttcccact 360gtcaaataga atataagcaa tgatacaaaa tgttgagtct tcatcctcta actccagatc 420ctggtatatt gccctacatt tctatacatt aatactaact tatacactga atacaagagt 480naaaccaact gtcngggctt aatanggnga aaatgctctt gncctaaanc accagggtgg 540ctnggtttat tcctacatgt ggactaaaan gnaatcatct ttatggcngg aaana 595 143 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 143actactcgat tgtcaacgtc aaggagtcgc aggtcgcctg gttctaggaa taatggggga 60agtatgtagg agttgaagat tagtccgccg tagtcggtgt actcgtgtga agttggcagg 120gacggttcct gtcatcttct tgggcttatt tggtgtgctg ttgaaggggg gagactagag 180aaatggcagg gaacctctta tccggggcag gtaggcgcct gtgggactgg gtgcctctgg 240cgtgcagaag cttctctctt ggtgtgccta gattgatcgg tataaggctc actctcccgc 300cccccaaagt ggttgatcgt tggaacgaaa aaagggccat gttcggagtg tatgacaaca 360tcgggatcct gggaaacttt gaaaagcacc ccaaagaact gatcangggg cccatatgct 420tcgaggntgg aaanggaatg aattgcaacg ttgtattccn aaagaagaaa atggttggaa 480gtaaaatgtt ccttatgacc tcncaacctt ataaacncat ccgtttnttt acaaccntta 540accacatggg aagttcattn aaaaaaactg aaaactttgn aaagnttttt ttnnccttga 600aaagggaact tacctcgccc 620 144 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 144cgaggtactt tttttttttt tttttttttt ggggtcagtg gtgatatccc cctaatcaat 60tctgattgng ttccttttaa tcttctctca tttctttttt attagactag atagtgattt 120atctatttta ttaatttttt caaaaaatca cctcctanat ttgttgtttt ttaaggggtt 180ttatgtctct atctccttca gttcaactct gatcttggnt atttcttgnc ttctgctaga 240tttggggttt gntttctgnt ggntctctaa gttctttttg ntgngacatt agattgncaa 300cttaaaatct ttctagctat ttgacgtggg catttaatgc tataaatttc ctggtaacac 360tgctttcgct gtatnccana naatctggga tggtggggcc ttggtttcaa taanttccaa 420tacctcttaa gggggnggag ccaanaagan ctaatagggg cagcactgct ctgggctncc 480atcaanaagg acaaaaactg ggagngaccc tgcttnttca ctgaggnacc ggcccggccg 540gccgtccnaa ggcgaatcca cncnctggcg gccgtctatg gatccacccg gnccaactgg 600ggaatatggc aaa 613 145 345 DNA Homo sapiens 145acactgatct acaaaaattt taaaatgagc cgggcgcggt gactcacgcc tgtaatccca 60gcactttggg aggccaaagc aggcggatca tgaggtcagg agatcaagac catcctggct 120aacacggtga aaccccgtct ctactaaaaa tacaaaaaat tagccgggtg tggtggcggg 180cacctgtagt cccagctact cgggaggctg aggcaggaga atggcgtgaa gccgggaggt 240ggagcttgca gtgagccgag atcacaccac tgcactccag cctgggcaac aaagcaagac 300tctcaaaaaa gaaaaaaatt tttttttaaa tgagctgggt gtacc 345 146 475 DNA Homo sapiens misc_feature (1)...(475) n = A,T,C or G 146actacaaggt ttagcatttg ctctgctggt cgacattccc ccagtctatg ggttgtatgc 60atcctttttc ccagccataa tctacctttt cttcggcact tccagacaca tatccgtggg 120tccgtttccg attctgagta tgatggtggg actagcagtt tcaggagcag tttcaaaagc 180agtcccagat cgcaatgcaa ctactttggg attgcctaac aactcgaata attcttcact 240actggatgac gagagggtga gggtggcggc ggcggcatca gtcacagtgc tttctggaat 300catccagttg gcttttggga ttctgcggat tggatttgta gtgatatacc tgtctgagtt 360cctcatcagt ggcttcacta ctgctgctgc tgncatgttt tggtttccca actcaaattc 420atttttcaat tgacagtccc gtcacacact gatccagttt caattttaaa agacc 475 147 629 DNA Homo sapiens misc_feature (1)...(629) n = A,T,C or G 147cgaggtacgc gggatttgaa tcttaaactg tatttttctc ttagtattgc taatgagtaa 60agaaaagtct cataaggtag ccaaatgaaa aagaatgaaa gggaaagtga aaaattaagg 120ggacaaaaga tgggatgtga aaagaagaat tctagtttga tggtgactca tattcacgat 180aggatacaaa gtgtgatttg ttggaaacat gtcccaaatt tctaaaattc tgcttctctg 240ccaaaagcaa tgtctttctt ggttgatatt tgagttttaa aagggtcaaa tctttctaat 300tttttgtatc tttagagggc agcactagaa gaaatcagca ggtctaatcc caccagtaag 360aaaactacca cttcttgatt tttacagatt taaaaaaatc ttttcagtgc ctttcttttt 420aatgtaaata caaatttaaa cctangctta atatagcgtt tccctttccc caagtgatgt 480cnaggtcgat gccaaatcaa tgatccnaaa tgatcgnggt naaaataact caaagggttc 540ttaaggngag tngcatgcca aaaaatacct tgattccggg ggtttggacc tggctttgtt 600ggggcctntg aaatgccaan ttancccan 629 148 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 148acaaaagagc ctgattcttt ttaattccac aaatacctag catctcaaag taacatgtaa 60acaaacttct atgctgctca atgaatcctt ccaatttcga taataaacta aatagtattg 120gatctagtat atgactttca tgtgtaagtt atggttctat ccattacttt aacaatatta 180ctgatgtaac agagaaaaat tttcaactat tgtatttatt taaaacaaac tgacaagttc 240aagcacctgt cttcagaaaa gccagcagca tttttttttt ttaacatact caaagtaaga 300tttggcctaa gcccttaata cctttctgaa cagccatgca actaaacacc ctcagggaga 360tgttacataa gggagagaag aacatggagc aatttgcact ttttccctag ataatattaa 420caaggnaaag caaatncaga tctttatgaa tgaatggntg gcatggttaa tcacttggac 480tttttaaact agagncncta tcatattggt aaatagaaan aaaggatttt aataaagctc 540tncctgcttc aaaattaagg ggacnttttc tgggaggctt tcagggacca taataaggta 600aaaggggacg gttg 614 149 628 DNA Homo sapiens misc_feature (1)...(628) n = A,T,C or G 149nccgaggnac tttnnttttt tttttttttt ttttnaacag cgncttttca tttttattac 60tcaaaaaagt ttcatttttt tatttaagct ttctgactct gngcttgggc cttcaacact 120ttcacaacga ttttctgctc ctcgataagg aaagcccgct tgatcctana aaggaaaata 180ccaaattaat catttcttta aaatgaactt cattttttat ttagcccaaa aaaggnaaac 240atggtaaaga accaagcnaa gcaatcaggg aacccaggaa actacnggat acccaaatac 300ngagtaaaac ttaaaagggg aaattcattt aaagcaggga aatccctcaa tttcatgccn 360gtagttatct gncctcctct gagcaagaat aactatgaag catcccccag gagaccacnt 420atgagactta attattggta ggatccagga atagnggnat ttnttgattt gcaaaangtn 480taaaaaattt taaccctntt ttgaaaattc ccagnaaaaa caccncataa ggggctntgt 540gttaaaacta aaattaaagg gaagggtttt tccagaaacc ccccccanac cagggtttna 600accggttang gcanntcncc aaaccnan 628 150 509 DNA Homo sapiens misc_feature (1)...(509) n = A,T,C or G 150ttggggaann aaaaaaaaac tttttttttt nggggnnngg ggntgcnanc natncaaaaa 60tcaaaancnt ntttgggttt taactttttt ttttttgntt gncaaannaa aantaaantt 120tntttttana tttgctaang ggccngancn gcnnaaaaaa nccttttttn ggggaanctt 180nggggcaaat tnnttnancn accctttggg anaacttttn ttaggggggn nnnaaccgnc 240atttttgccc acttttttcc cttttgntta anggggncct tgggcnggac cncccttagg 300ggnaattcac ccnctggggg gcgttatntt ggatccactc ggnccaactt gggggaaaaa 360gggaaaacnt tttctggggn aaattttttc ccncnaaatt cccaanaana aaaccggaac 420nnaaanttaa acccgggggc ccaaggnggg ccnncccntt nttgggtggg ccctgcccnt 480ttaangggaa attttggccc tttttaaaa 509 151 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 151ggtacttttt tttttttttt ttttttttgc tttggacaaa tttattgaaa catacaggcg 60gctgttagca gagaaatcat tccatgattg atgtgttaca tttggccact accttgaatg 120tataatttaa aaattatatt tttcacaact aagcctttgg ccaaaaaagt catttagcac 180atctttaaag atcaataaga aatggatttt ggacattaaa aagatcaagt cactgaatta 240aacagtagca acccccatta atctagaatc ccatagtgct gaaggtagag gtgtctgtgc 300aaagctagtc atttgttaac agcaatcana aaanatgggg gcaggcacac ctgtcaaaag 360tggcaacana nctggcagga caggacggct gggctggtct ggtcaggtga gcatgtncca 420aaaacagcag caacagaaaa cccgtccacc angcttgtga agcangtgga tggtcctagc 480tcatctnttn ttttggnctt ntancacata cactgngggt ttangangnt tctgaggncc 540accttgccnc cctacctgcc cgggnggccg ttnaaagggg aattccacca ctgggggccg 600tctaatggga cccacctggg cc 622 152 313 DNA Homo sapiens misc_feature (1)...(313) n = A,T,C or G 152acggtggatt agttcttttc agcatgttcc ttctgtatga tacccagaaa gtaatcaagc 60gtgcagaagt atcaccaatg tatggagttc aaaaatatga tcccattaac tcgatgctga 120gtatctacat ggatacatta aatatattta tgcgagttgc aactatgctg gcaactggag 180gcaacagaaa gaaatgaagt gactcagctt ctggcttctc tgctacatca aatatcttgt 240ttaatggggc agatatgcat taaatagttt gtacgcgggg aaaaaaaaan aaaaaaaaaa 300aaaaaaaagt acc 313 153 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 153cgaggtacgc gggagggcaa caagaaccat ttccaatcta gaggaaagct ccccagcatt 60gcttgctcct gggcaaacat tgctcttgag ttaagtgacc taattcccct gggagacata 120cgcatcaact gtggaggtcc gaggggatga gaagggatac ccaccacctt tcaagggtca 180caagctcact ctctgacaag tcagaatagg gacactgctt ctatccctcc aatggagaga 240ttctggcaac ctttgaacag cccagagctt gcaacctagc ctcacccaag aagactggaa 300agagacatat ctctcagctt tttcaggagg cgtgcctggg aatccaggaa ctttttgatg 360ctaattagaa ggcctggact aaaaatgtcc actatggggt gcactctaca gtttttgaaa 420tgctaggagg caaaaggggc agagagtaaa aaacatgacc tggtagaagg aanaaagcaa 480aggaaactgg tggggaggat caattagaga ngaggccctg ggatccncnt nttcntaggn 540ccctctcata cnaaggacac tttttatatg ccttcccaaa ctgntnggga agggtnaaac 600caaaatccgg ggtanaacct 620 154 339 DNA Homo sapiens 154ggtacctgga ggatatagac ctgaaaacac tggagaagga accaaggact ttcaaagcaa 60aggagctatg ggaaaaaaat ggagctgtga ttatggccgt gcggaggcca ggctgtttcc 120tctgtcgaga ggaagctgcg gatctgtcct ccctgaaaag catgttggac cagctgggcc 180gtccccctct atgcagtggt aaaggagcac atcaggactg aagtgaagga tttccagcct 240tatttcaaag gagaaatctt ctggatgaaa agaaaaagtt ctatggtcca caaaggcgga 300agatgatgtt tatgggattt atccgtctgg gagtgtggt 339 155 450 DNA Homo sapiens misc_feature (1)...(450) n = A,T,C or G 155cgaggtactt tttttttttt tttttttttt tttttcntat ttttgtttaa tttatttaan 60accacctnct tacaacttnc anagagaaaa tacaaaacaa gaaacanact tggtttnaaa 120 156 760 DNA Homo sapiens misc_feature (1)...(760) n = A,T,C or G 156cgaggtactg ccccagtgaa aatggaactg aaagagcctg tagctgtcag agaaaggacc 60acctttcagc actgatcggt tatcgttgtc ctcaaaattt acatggaagg aatgccccac 120attgataatt tctttggctg tggctgggtt gtaggagaca ctaataggtt tcagagaggt 180gtcatgtttg gtttcactgg ttttaatatc aacaggggac tggttatttc cattggcaat 240gggatacagc ttgctccatt gttcaggacc atttttgtca tcatatcccc agtctggact 300tgccattatc ttctactgag ttttcttttt ctgaaaacaa aaataatacc tggaataact 360aactgccccc gcgtcctgcc cgggcggcca aaggggcaat tccaccactg gcggccgtac 420ttatggatcc aactcgtccc ancttggcgt aatatggcat aactgttctg nggnaaatgt 480atcccttaca attcccncac atcnacccga acctaantgt aancctnggn gcnnataagg 540actactnctt aatgggtggc tctgncnttt caannggaac cttngcnctn gntatgattg 600ccaccccgga naggggtggt ttggccttcc ntcttgtann aatcttcncg gnttgttgga 660anggtnntct taggggatng ttccaatggg gaccgnaanc ttccagccna ggcaccaaan 720cnttggttta ncccccacnn aaaantanag gggncngggt 760 157 668 DNA Homo sapiens misc_feature (1)...(668) n = A,T,C or G 157ggtacccagt agtcattcag gaacaggttg ttcagtttcc atgtagttga gcggttttga 60gtgagtttct taaacctgag ttgtcgtttg attgcactgt ggtctgagag acagtttgtt 120ataatttctg ttcttttaca tttgctgagg agtgctttac ttccacctat gtggtcaatt 180ttggaataag tgagatgtgg tgctaaaaag aatatatatt ctgttgattt gaggtggaga 240gttctgtaga tgtctattag gtctgcttgg tgcanagctg agtcaattcc tggatatcct 300tggtaacttt ctgcttgttg ntctgtctaa tattgacagt ggggcgttaa agtctcccat 360attattgtgt gggagtctaa tctctttgta ggtctctaag gacttgcttt ataaactggg 420tgctcttgat tgggtgcaat atatttagga tagttagctc ttcttggtga atggancctt 480taccaatatg aatggcctcc ttccttttga ccttgtgggt taaagctggt tatngaaact 540ggatggancc ctgctttttt tggttcattt cttgnagggt cctcagcctt attttancnn 600gnggctttgn cccncntccg cggcnttaag ggaaccacnc tgngcgtcta ngancactgg 660caactggg 668 158 737 DNA Homo sapiens misc_feature (1)...(737) n = A,T,C or G 158tttttttaag ggtcaatgtt tacatttttt tcatataaat atcaagttgt cagcaccatc 60tgttgaaaaa aatctttgta atggctaatc ttttatgtca ttagatttga taatagttta 120agaatttttg ttcctatatt catgagggtt gctttccttt aacttttttg ttttgtaatg 180tctgtgtcag gntttactat tagaacaata ctagtctagt aaaaaaaaaa anaaacaaaa 240aactancaag tgtntctccc cttctattta taanaanggn gttacttctt ccttaaatgg 300nnaaattatg agngaaactt ggagtatcnt tgcnggantg gaagtttcct tgtggaaaga 360attttatnat nattacattt caatagtncc gcntccctgc ncgggcggnn ntcaaaggcg 420aatncagcaa attgntggcc gntactnngg accaacntcg gnccatnntg gggnancang 480tcaanctgtt ctngnnaatt gtncccttcc aatncccaca nanaaccgaa cctaaatgga 540acccnggggc tantaangnc taccnntatt gngnggctnn gcccttnnnt ggaaactgnt 600cnaccnttat aatggccccc cnggaaggnt tntttggcct tctnntncaa anctggcngg 660nttntgtgna ggttatctna ntggatgttc cacgggaacn gaanatntan ncagtggacn 720aaanntnntn ttttnct 737 159 739 DNA Homo sapiens misc_feature (1)...(739) n = A,T,C or G 159cgagggtaca ctgtgagaga ataacatgga cttgatatgg catcacactt gttttaaagc 60aaaaaaaaag aaaaaaagaa aaaaaagaaa gtacagttaa aaagtaagca ttgtagtaaa 120tagtggattc tctggtgtgt attttttatc tcagtgttga aaattggaaa agaatgggct 180gaagtctaaa aactggaata atgaaggaca ctaaatgcct ttattgtaga tactatgttt 240gtaagtctat agctaagcaa cttaagccaa aaaggtcttt caactgaagc tttaatcaac 300ttattttgga gatgttctct tccttatctc atgcgtcatc cctaaaataa taagatacat 360gggatcaaat aacccttgcc ttttcaacac aaatcagttg gaaaattatg ggttgagtcc 420tgttgctgcc atggttctgt tctcaaaatg agtgtgtatg acatcccatc tatgtaatag 480gctacctttt tggctcttgg aactttgtcc tgccggccgg ccnttaaggc nantcnacca 540ctggcggccg tactatgggn tccagctcgt ccaaccttgc tatcntggct acttttctgg 600ngaatgtatc cgtncatccc cacttcancg gagctaangg aancntgggc ctatggggct 660actccatatg ctngccnctg cnttcnangg aacncgcntc ttaanatgca cccnggaagg 720gtngtngcct tcnttcttt 739 160 802 DNA Homo sapiens misc_feature (1)...(802) n = A,T,C or G 160cgaggtacag cagagacctt cctgcttttt actggggact ccagattttc cccaaacttg 60cttctgttga gatttttccc tcaccttgcc tctcaggcac aataaatata gttataccac 120taaaaaaaaa aaaaaaaaag tacgcggggg cccattgttt ttgtaatctc tgaggagaag 180cagcagcaaa catttgctag tcagacaagt gacagggaat ggattccaaa caccagtgtg 240taaagctaaa tgatggccac ttcatgcctg tattgggatt tggcacctat gcacctccag 300aggttccgag aagtaaagct ttggaggtca caaaattagc aatagaagct gggttccgcc 360atatagattc tgctcattta tncaatatga ggagcaggtt gactggccat ncgaagcaag 420aatgcagatg gcagtgtgaa gaaagaaaca tatttacctt taaagcttgg tcccttttna 480tcgaccnaag tggtccgaca agcttggaaa attactngan aaagctcaat nggactatgt 540gactcttttt aataatttcc anggntttaa acccgtgagg acttttcccc cgntaaatgg 600aaagtatttt gcnannggac ttgacttccc ggngccntaa gngaattcac cactgggggg 660gnttagggtc cnnntggnca anttggnaaa ngggtaatnn cntgnaatgt tcctcatccc 720aantngccgn ataantaacc gggcaaaggg cccaaatggn gccctccttn nngaatnanc 780cctntannna ancggggggg gg 802 161 214 DNA Homo sapiens misc_feature (1)...(214) n = A,T,C or G 161acttttnntt tattcnttat ttttgggacc tgctctcact gtccacccag actggagtgc 60antggcacca ttatagctna ctgcagcctt gacctnntgg gctcaagtga tcctnctgtc 120tacacccccc aagnatgntg tgacattatg cttggataat acttgtatnt tangtaaaga 180cagggtcttt ccnatnnacc nggnagatct naaa 214 162 304 DNA Homo sapiens misc_feature (1)...(304) n = A,T,C or G 162acttaggaat acaactatat acatatgatt ttatttttaa gaccatatta tatttgggta 60tctactaata ttttgtataa agcaattttt tgttccatta cgtgactttt tgttttattg 120tatatgtaat ttaacacaca ataaagggta aagttgcttc cccaaaccac acttttaatc 180aaaacctaga atcatctgca gtccttgtta aaaatgcagg tttctagaac cctctgaagt 240tctgattaaa taaatttatt gcaaatcaaa naaaanaaaa aaaaaaaaaa agnccccggg 300gnta 304 163 461 DNA Homo sapiens 163actagagcca gtcatcctta acaaatcttt tcacatttta tttctttcac atgtagtcat 60cttcaaaaag gaaagatttg gaattttaga aaaggggcaa ctcttctttt tagcattctc 120atcagaaagt cacaaaaatc gatggaatca tttccactgg gaagattgac cttttgtatt 180tatttgtggg gtaaattaat aagcattcca gatgcttgca gcttcctgca tccaggagat 240gctgtgttcc ccgtgatgca gctggaaccc aagctgcagc aggagatgca agtttcagga 300tgttccccac tgagctggag gaatatctac agcagtgatg cttgaaattt tgtatgaatt 360attttgtcgc ctaccctttt cctccaaaca aaaattagag gattatttaa tccttgggat 420cttccccttt ttgagaaata aagtttttat caaaaaaaaa a 461 164 345 DNA Homo sapiens misc_feature (1)...(345) n = A,T,C or G 164tttttttgag acaaggtctt actctgtcac ccaggctgga gtgcagtggc atgatcttgg 60ctcactgcac cctctgcatc ccaggttcaa gtgattctcc tgtctcagcc tcccttgtag 120ctgggattac agccacttgc cactgcaacc ggctaatttt tgtattctta gtagagatgg 180ggttttacca tgttggccag gctggtcttg aactcctgac ctcaagtgat ccacctgcct 240ccatgtccaa agtgctggga ttacaggcat gagccaccac ccctggccta agtcattaat 300ttaaaaaatg ttattaggat gancgacctg ccgggcggcc gntaa 345 165 385 DNA Homo sapiens misc_feature (1)...(385) n = A,T,C or G 165actgaaacag aaactntacc caattgcagt ccatatgttt tctgggatcc cggagttccc 60tttcaacaat gtaaaataca nacttaggtc aaaagttccc atgtctgaga aaactcaagc 120caaatcagtt ctcctccaaa gttgacagga tttatgcttt aaaaatagag atacagaatt 180ctctttggaa agatctacca aattcctgta agaaacagtc tacccaaagt aggggaaagg 240ctatatgana agttcaaggc acttcttaaa aatatatctt aggttttagg gaaaggaaac 300agacaagttt ccagacccgt gggtggaatg gatgtagcag atcactgaga ggttacaagc 360gccgacctng gccgngacac gctan 385 166 745 DNA Homo sapiens misc_feature (1)...(745) n = A,T,C or G 166tttttgacga tgtctctcaa caatacctga agtttctcat actcatcatc ccaagtctga 60aaaacttcaa agcatgctac cataactttt tcaaattctt cataagcaac atgcatcaat 120ttcctagtgc ccaatacttt gagtaattga gaactcaagt ctcttgaaat tgcctccacc 180aaacgcagtg ccctctgaat aggatatttt gtgtttcgga tctttctcaa atcccgcgta 240ctttgagaag ctgaggcggc agatcacttg aggccaggag ttcgagacca gtctcgtcaa 300catggcgaaa ccctgctcta caaaaaaaaa aaaanaanaa aaattagcca gacatggngg 360cccacatctg tagtcccagc tacttganan gctgaggcat gagaatagct tgacctggaa 420nggcaaaggt ttantgancc caaactgngc ctggattcca atnnggngga cccagtgana 480tttgtctcaa aaaaangaaa ggaaaaaaga gcccgncgga aggaaggatg gattgangga 540aaattgtggc ctccnnnnaa aggnccaang gccctnangt ttctttgaat agtttccctn 600gccnttctta ngggcctnng ccttttttcn nnctggcgaa cctaggnatt cacatggggg 660ttangacncc gccnctggga naggaaagtn ctggaagnnc ncntcccaat ancgnntang 720aacgggcngn ggannaattt tttnc 745 167 623 DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G 167accagccact gcaaaaacat gccaaattgt aaagaccatc gaggctggga agaaactgca 60tcaactaacg agcaaaataa ccagctaaca tcataatgac aggatcaaat tcacacgtaa 120cactattaac ctgaaatgta aatggactaa attctccaat taaaagacac agactggcaa 180attggataaa gagtcaagac ccatcagtgt gctgtattca ggagacccat ctcatgtgca 240gagacataca taggctcaaa ataaaggaat ggaggaagat ctaccaagca aatggaaaac 300aaaaaaaggc aagggttgca atcctagtct ctgataaaac agattttaaa ccacaaagat 360caaaagagac aaagaaggcc attacataat ggtaaaggga tcaattcaca agaagggcta 420ctattctaaa tatatatgca cccaatacag gacccccaga ttcatgaagc aaatccttga 480gattnccaaa ggattaactc cncccngtat tatggagact tncacccact ntnacctttc 540ccgatcttgn cccaaagtac cnggtttccc gaattgactn gtttgncann gggctattaa 600tttngaattt cncccaaaaa aaa 623 168 703 DNA Homo sapiens misc_feature (1)...(703) n = A,T,C or G 168ggtactccct gtttgctgca gaatgtcaga tattttggat gttgcataag agtcctattt 60gccccagtta attcaacttt tgtctgcctg ttttgtggac tggctggctc tgttagaact 120ctgtccaaaa agtgcatgga atataacttg taaagcttcc cacaattgac aatatatatg 180catgtgttta aaccaaatcc agaaagctta aacaatagag ctgcataata gtatttatta 240aagaatcaca actgtaaaca tgagaataac ttaaggattc tagtttagtt ttttgtaatt 300gcaaattata tttttgctgc tgatatatta gaataatttt taaatgtcat cttgaaatag 360aaatatgtat tttaagcact cacgcaaagg taaatgagca cgttttaaat gtgtgtgtgc 420taattttttc cataagaatt gtaaacattg actgaacaaa tacctatatg gattggtaat 480gacttatgag caanctgctt ggccagacag ttacccaaac tttatatatn tnngaaggta 540tacactgnga aatctctggc taancgaatg cntccagggg taanngggtn tggntggant 600aaanaatgcc ctgcaaaaaa aaaaaaaaaa aagccttccg nggccttnaa nggaatcnnc 660angggnntnn ggccactggc cactggnaaa ngnaacgtct gga 703 169 609 DNA Homo sapiens misc_feature (1)...(609) n = A,T,C or G 169acgtccatct tccagctgct tgccagcaaa gatcagtctc tgctgatcag gaggaattcc 60ttccttatcc tggatcttgg cctttacatt ttctatcgta tccgagggtt caacctcgag 120ggtgatggtc ttaccagtca gggtcttcac gaagatttgc atcccacctc tgagacggag 180caccaggtgc agggtggact ctttctggat gttgtagtca gacagggtgc gtccatcttc 240cagctgtttc ccagcaaaga tcaacctctg ctggtcagga gggatgcctt ccttgtcttg 300gatctttgcc ttgacattct caatggtgtc actcggctcc acttcgagag tgatggtctt 360accaagtcag ggtcttcacg aagatctgca tcccacctct aagacggagc accaggtgca 420gggtggactc tttctggatg ttgtaatcag acanggtgcg ttcatctttc actgnttcca 480caaaaaacaa cctctgctgg canganggat ccttccttnc ttggactttg cctgacattc 540tnatggngta ctccgctccc ttcaaaggga tgncttacan tcanggnctt acnaaaattt 600cntccnctt 609 170 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 170acaaagaaca tgtagctata ggaaataata gtgtaaatag cagtatataa actggcccat 60gtaaaataca aaaatattca ctgaagtcag gttttctata aaacagtgtt tattagaggt 120attttactat gaatcaggca tataatctga atgtagaaac ttttagaaat attaacagca 180ttcagtcagt gccatgcact tgtgcttcca attatttttt taaagctgct ttgttttgac 240tcatgtgaaa tagttaaggc ctacattctt atacacatta tccatcttac aaggttaaca 300attttacact aaaacacagt ttaaattaaa aacgattttg aaaaattaca tctatattta 360atccctaaga agtgttttaa gctggtaatg cagctcgctg tagctctaag agaggggtta 420gtcaggaatc tgatcttgag ccataaangg tttcaggcta aacaaagaac aaatttaagt 480gacagaaaat attataattn caatatactc agttttttgg tataaaatac cctgctagca 540tgccactggc tatattgngg gcataatata aaatgncggg gggggggatg gancctccaa 600gncaaanttt ggaccca 617 171 621 DNA Homo sapiens misc_feature (1)...(621) n = A,T,C or G 171acagtatggg ggttgtaaat tggcatggaa atttaaagca ggttcttgtt ggtgcacagc 60acaaattagt tatatatggg gatggtagtt ttttcatctt cagttgtctc tgatgcagct 120tatacgaaat aattgttgtt ctgttaactg aataccactc tgtaattgca aaaaaaaaaa 180aagttgcagc tgttttgttg acattctgaa tgcttctaag taaatacaat tttttttatt 240agtattgttg tccttttcat aggtctgaaa tttttcttct tgaggggaag ctagtctttt 300gcttttgccc attttgaatc acatgaatta ttacagtgtt tatcctttca tatagttagc 360taataaaaag cttttgtcta cacaccctgc atatcataat gggggtaaag ttaagttgag 420atagttttca tccataactg aacatccaaa atcttgatca gttaaaaaat ttcacataac 480ccacttacat ttaccaactg gaagaataat caatctctca agcatgggat tattagaatc 540aacantttga aagctgtcct tgaaggctaa taaaaaagnt tgtctaacct ttcatgaggn 600cttnttntta ctnccttacn g 621 172 399 DNA Homo sapiens 172actcaaaatt acacatttgt ttaaataaat atccacacaa attctcagtt acatcaagta 60gctggtttat atttagatta tctcaagtag gggggaataa ccatgtgtag gaattcatag 120aaaaataaac aatcagctga agaggtctaa gaaaatgctg acttttaaaa tttcacttat 180tttccttgaa gttttctacc cttcccatcg atgataaacc aagatcatgt aatggaaaat 240ttcaaaccag ggctaaattc taaagtaaag cttcaattca agcccttccc ccaagagaat 300taattttcct gatttctctt tctctcacat ctaaggagaa cattttaggc agttaaattt 360cagaacttca aggtttcatc agggtcacct ttatgtacc 399 173 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 173actttgtgga taagaaaatg gaggaacaca tctgatggag agtgggcatt tgacaacaat 60ggaacaggta acctgcatgt aaaatcaaaa tataagtgtc tttttaagag ctgaaagctg 120ctgctggtca ttcattaatg tgtcagacat ttaatcagga tgctggacct tcaaaataac 180tgaaaaaaga accaagaaaa ggcgtttttg ttttcaacaa actttactaa ataaccctgg 240aaaggcaatg aacgatctga caatttaagc tctaatgatt taaagctcag ctagaagaaa 300gtgaggcatg acatatactg tcaacggagg gtgaaggagg canatttctg gaaatgcaat 360gatcccacca tttgcttcaa ngagaaacct gcanacatat tttcangtct tgntaagtna 420caactgtnta tttgtaatca atcatttngg aaaagtctgc tatgtaactt angncactgt 480gcccccnacc accgatgaaa aggaaaaacc cctgacacca ggaaaatcct tccatcctca 540aanaaattaa gngaccaacn tttaaagaaa aaaaatnanc ccncctctnt ttacaaatnt 600ttcntccaaa tnttcn 616 174 631 DNA Homo sapiens misc_feature (1)...(631) n = A,T,C or G 174ggtacgcggg gacacgcacg ccgggcgtgc cagtttataa agggagagag caagcagcga 60gtcttgaagc tctgtttggt gctttggatc catttccatc ggtccttaca gccgctcgtc 120agactccagc agccaagatg gtgaagcaga tcgagagcaa gactgctttt caggaagcct 180tggacgctgc aggtgataaa cttgtagtag ttgacttctc agccacgtgg tgtgggcctt 240gcaaaatgat caagcctttc tttcattccc tctctgaaaa gtattccaac gtgatattcc 300ttgaagtaga tgtggatgac tgtcaggatg ttgcttcaaa agtgtgaagt caaatgcatg 360ccaacattcc agttttttaa gaaagggaca aaaggtgggt gaattttctg gagccaataa 420ggaaaagctt gaagccacca ttaatgaatt aatctaatca tgttttctga aaacataacc 480accattggct atttaaaact tgtaattttt ttaattttcc aaaatttaaa tttgaanact 540taaccccant tgccatntgn gtgacaataa aacattatgc tacccntttt aaaaaaaaaa 600aaaaaaaaaa agtcctgccc ggcggccctc a 631 175 261 DNA Homo sapiens 175acgaacctac agttttaact gtggatattg ttacgtagcc taaggctcct gttttgcaca 60gccaaattta aaactgttgg aatggatttt tctttaactg ccgtaattta actttctggg 120ttgcctttgt ttttggcgtg gctgacttac atcatgtgtt ggggaagggc ctgcccagtt 180gcactcaggt gacatcctcc agatagtgta gctgaggagg cacctacact cacctgcact 240aacagagtgg ccgtcctaac c 261 176 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 176cgaggtactc tgccttttag gagatgaggt aagacatata catagatggc ttttactagc 60caaggcaatg taaatggact aagattctca tgtgacttga ggttatctga tgaatttatt 120ctcttcaaaa ccacctacct ttagagggca tgtttaaccc ctctctttat ttaaggaggg 180agagaaaaac acatgtaacc agaattcaga gtgggttact caacctaaga gaacatacgg 240agttctcttt gggaaaacaa caagactaca gtgttcactt cgcaccatga agtggcactc 300ctgttatggc tgtcagagtc ctctcacttc ttatgaaagg atgcatctga ttctgaaatt 360actgatatat tcgatcagtt anggatgttt taaaaagtga aaacaaatgc cacacataca 420ctttctagct ttcttgaaat cacccgacac attccaaaaa tagagaattc cctattactt 480ttagagaaat ttccatatan tcttggtnaa gaanccagtt gngcntattc caatttcagg 540gtcttggttt ttgcccaaac ccaagtgttt ccntntttta nggcttttca tggccgattt 600naaaccttnt ttgtgg 616 177 632 DNA Homo sapiens misc_feature (1)...(632) n = A,T,C or G 177cgaggtacag gtcagagtct tcttttcttt tctttttgag atggagtctt gctctgttgc 60cagactggag tgcagtggtg cgatctgggc tcactgcaat ctccacctcc cgggttcaag 120cgattctcct gcctcagcct cccgagtaac tgggactaca ggtgtgcgcc accaagccca 180gctcattttt gtatttttag tanagatggg gtttcacggt gttggctagg atggtctcga 240tctctggtca gaagtctttt ctgtaaatat ccttggtaaa gaagcaattt tagactgtag 300ctgttgcaaa tgctttaagg aagaagcaaa acaactgtca gtcttcctga aatgaaaaaa 360ctacaccagg gctgctatat caaagcaacc ccaaccagca cttcaatcat gatgcccaca 420gtggccccac tgagaaacca agaaaagttn cagatacaaa actgngatgc tcttgctatg 480gnaatattgc nggcngtanc caagttagaa accaaacaag cntanggccc cgttnttttt 540tggcgtgatt ttggcaanaa aaaaaactgg gngngtggtg ngggttccca ttgtaccccc 600aaaaaacttn gggatgggtt aaagcccnng gc 632 178 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 178actttntttt tttttttttt tttttttttg ggatttagtt tttatttcat aatcataaac 60ttaactctgc aatccagcta ggcatgggag ggaacaagga aaacatggaa cccaaaggga 120actgcagcga gagcacaaag attctaggat actgcgagca aatggggtgg aggggtgctc 180tcctgagcta canaaggaat gatctggtgg ttaagataaa aaacaagtca aacttattcg 240agttgtccac agtcagcaat ggtgatcttc ttgctggtct tgccattcct ggacccaaag 300cgctccatgg cctccacaat attcatgcct tctttcactt tgccaaacac cacatgcttg 360ccatccaacc actcaatctt ggcagtgcag atgaaaaact gggaaccatt tgtgttgggt 420ccaacatttg ccatggacaa aatccangac ccgtatgctt taagatgaaa ttctcatttc 480aaatttcttc ccataaatgg acttgccnca tgccatnttg ggtgtgaagt nccnccttgc 540ncataaccct ggaatatttt tgaaacagaa ccttttacca atcntttttt catgttaaaa 600acnaaaattt t 611 179 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 179acctcaattt tatcatttta gagtatttgt tagaatagga tctctccaaa atcaaacagg 60atcaatctgg tcacgtctaa tcctaagaca aaacactatg taaaattttc ctgtatctaa 120atgttgccct ctaggtaaat ctgtgatatt ttagagactt tcttttgtgg aaaaggtaat 180ctgataaatg ggaagagatc atcagacaag ttcacaaata accattattt ctgcagaatt 240cagttgaagt tggttttttg taaatgctta ttgggaattt ctaaagcact gacttggaga 300ggccaagagc ctccatcaat ccctgcttgg atagccactc ccgttactac tgctaggtca 360gggtctacag atgtgttggg atcttttcca aagaactctt gaatgacttg acggatccga 420ggaataccaa tggagccccc aactaaaacc acctcatcaa tctcagtctt ttncaggtgg 480ncttcttcaa tctcctgaat gggacctcgg ccgcancacn ctanggcgaa ttccacacct 540ggcggccgta ctaatggatc caactcgnac caacttgggg aacatggcta gtnttcnngg 600ggaaatgttt c 611 180 621 DNA Homo sapiens misc_feature (1)...(621) n = A,T,C or G 180acccttaaac tggcaggaca tttttgaaat cacaaatttg cacataaaga atgtcacgaa 60cagccatgta tccatataca gcaatcaaat aaggaactta tgacctaaag caaaggtaaa 120ctttcttgaa acttaacatt ctataccaac taggcaacct ctgcccagga tgagagttgg 180atttttcaaa aacctctaat ttaatagtgc agcatttcgt tttccctgat ggcctgtgtt 240tcacagcagt ttttaaaaac tgcttgttca actatagctg cagcctatat cccagctatg 300gaaaaaaaag taaatcttag ttcaattttt gccagttgtt tctgtattta aatttaaaaa 360aaaacacact tccgctgggc aggtttagag ggttattatc aagtctgtgc ataactaaaa 420gttcaaagca aattcaattt tgcttaangg aacattgnna aagnacaatt cttggnanta 480catgcctcgt tgatccattt naancatana aaattcaccc ttgtgtactg gttcaagaaa 540aaaaccgatt tgacagttaa acatnttaaa anccccaacc tntgaagttc aaccaaactg 600ganttttgtt cctcgcccga c 621 181 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 181cgaggtacag accagagaca aagcaagaga agaagcagag actgttggcc cgggccgaga 60agaaggctgc tggcaaaggg gacgtcccaa cgaanagacc acctgtcctt cgagcaggag 120ttaacaccgt caccaccttg gtggagaaca agaaagctca nctggtggtg attgcacacg 180acgtggatcc catcgagctg gttgtcttct tgcctgccct gtgtcgtaaa atgggggccc 240cttactgcat tatcaangga aaggcaagac tgggacgtct agtccacaag gaagacctgc 300accactgtcg ccttcacaca ggtgaactcg gaagacaaag gcgctttggc taaactggtg 360gaagctatca ggaccaatta caatgacnga tacnatgaga tccccctcct ggggtggcaa 420tgtcctgggt ctaaatctgt ggcttgtatn gccaacttcn aaangcaaag cttaaaaact 480tgcncttaac tngggtnaat gtactncccg gcggccgttg aanggcaatt caacacattg 540cggccgtcta atggntcanc ttggnccaac ttgggnaana tggnaaannn ttcttgggna 600atttnn 606 182 610 DNA Homo sapiens misc_feature (1)...(610) n = A,T,C or G 182ggtactcata aaaaaagtct taccccaaaa ttgcaaacaa atacattaaa agattagaag 60aggtgataga aagcaccaga cattaaacaa aataaaaata ataaaataaa ttcaactcaa 120aaggtcccca ttcagcaaat actttgtaaa gtatggcctg tatgtaaata gtgctaaatc 180aaggactttt tagcagaaaa ttgctcggtt cttttatcta aggcttgaat ttgtaaagtg 240aaggcataaa agttaccaaa cattaagtaa ctcttaaaat ggcacacagg ttttaaagct 300attggttttt ccttcctaac tctctgaatt tttcccatgg cctttgtaga tcaactattt 360caaacgtatt ttacaccagc aactctcaac atacttgtct ttcagatatg tcatcagtca 420tgtctaacag gccaatagcc aaataacnga tttaaaacaa tncttaacta gctagcagga 480cattactttg gatctgctta ctgcaactga ctatttgtaa gcttaaaatc antttaatcc 540tgatacagaa acctcatctg cncatacntt actttggcct tcaaccttta aaaatactta 600atcccccgnc 610 183 608 DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 183cgaggtactt tttttttttt tttttttttt tttttatttt tttttttttt tttttttttt 60tttttttggg agncagctnt ttaattaggn tcttaaaaca tttaaaacnc caatttgnga 120ggataaattc cattcgtcan ancaaacnca aatcgcaggt anccctggan ctgaggaata 180nctttgattt ttggnaaaat ttgngagtcc acagctttnt gatcaatntt gcnctgctcc 240gnaatctcat atttctnttt ttctgngncg aaaatctcac cttcctggng tntgggcttc 300cgcagcttnt tntttttgaa gtaagcatca ataaaangtt ttgggatttt tacattgctg 360aaatccattt tgggtgaagg ggcaatgaca aatttntngn gtnttctttt taaaagaacc 420tcattggggg ccnaaggncc cncccaaatt ataaacccct ttccccctgg tttangnaaa 480cccccctttg ccctgngggg nccangagga taaanaaagg ccccggggaa gctggcccca 540ntttttcccg ccgncgaagg gttttgccgg ctaaaanttt tngggcattt nnngggnaat 600tttggctt 608 184 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 184acagccctga tgcaaagttt cagagcatga ccagcaagtg gccagctgtg tgggtcaaga 60tcagctccag ctgggtctgc ctcctgcttt acgtctggac ccttgtggct ccacttgtcc 120tcaccagtcg ggacttcagc tgaacctctg agtgccaagg acaccactgg aactcacaaa 180ggtctccttc accgaaaacc catatacctt ttaagtttgt ttcaactaaa atattaagtg 240aatgctttgc aagtttgact gtatgcaggt ttatatcaag aaggtgagat tgaataatgc 300ttgatgcaga atcgaaactt ctcatttatc tgnatattat gtttacttct aaggatatag 360cacaaaggga acattttttg tttaaagtga actacagctg tgctgtgaag agagttcttt 420ataaagcctg taggtctttt aactttggtt aaaatgtaag ataggaaaat gttggatatt 480tgaggcntgc ctaatatatt tatattggag natcctttna aagccaaaaa aaaaaaaaaa 540aaaaaaaagt nccttggccg gaccncccta aggggaattc cacncactgg gggccgtntt 600atggatccaa ctcgnaccaa ct 622 185 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 185acgcggggac agtcccaccc tcacacgatt ctttaccttt cacttcatct tgcccttcat 60tattgcagcc ctagcagcac tccacctcct attcttgcac gaaacgggat caaacaaccc 120cctaggaatc acctcccatt ccgataaaat caccttccac ccttactaca caatcaaaga 180cgccctcggc ttacttctct tccttctctc cttaatgaca ttaacactat tctcaccaga 240cctcctaggc gacccagaca attataccct agccaacccc ttaaacaccc ctccccacat 300caagcccgaa tgatatttcc tattcgccta cacaattctt cgatccgtcc taacaaacta 360agaggcgtcc ttgccctatt actatccatc ctcatcctag caataatccc atccttcata 420tatcccaaca acaaagcata atatttcgnc cactaagcca atactttatt gattctagcc 480ggagacctct nantntaacc tggatcggag gaaaccagta gctacccttt accaatantg 540ganaagaaga tcgnaccttg gcgggacacc ttangggaat tcaaccactg gnggcggtat 600atgggacccn ccng 614 186 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C or G 186ggtactgatt ttaaaaacta ataacttaaa actgccacac gcaaaaaaga aaaccaaagt 60ggtccacaaa acattctcct ttccttctga aggttttacg atgcattgtt atcattaacc 120agtcttttac tactaaactt aaatggccaa ttgaaacaaa cagttctgag accgttcttc 180caccactgat taagagtggg gtggcaggta ttagggataa cattcattta gccttctgag 240ctttctgggc agacttggtg accttgccag ctccagcagc cttcttgtcc actgctttga 300tgacacccac cgcaactgtc tgtctcatat cacgaacagc aaagcgaccc aaaggnggat 360agtctgagaa gctctnaaca cacatgggct tgccaggaac catatnaaca atggcagcat 420caccagactt naagaattta agggcatctt ccacttttta ccaaaacngn gaacaatctt 480tttcnttact taacnaacnt gcttccatgg gagccgggng naatccaatc aagggcataa 540cccgggcctt atttggcnng atgggtcang gnaatancct gaccaggaaa cccctgnttc 600cttgggggga antttgttgn nccccac 627 187 256 DNA Homo sapiens misc_feature (1)...(256) n = A,T,C or G 187ggaccttttt tttttttttt tttttttttt ggaaaagaaa ggccttacat atttattact 60gaatccagcc aaccaacgtg ttcataacag attcagagag gaaaacacgt cgaaatctcc 120anatagtggt gacattttca gcttgatatg gtaacatgat cgtgaccttc anacagcata 180aatatgtgtg ccatctcatg tgcaattcct tatanaccca gcttggttct tctccaatgt 240ctccttttgg agttgt 256 188 523 DNA Homo sapiens misc_feature (1)...(523) n = A,T,C or G 188ggtaccacct acacccaaca agtcaatgag ggacttcttt ttaatttggt aggattttga 60ctggttttgc aacaataggt ctattattag agtcacctat gacaaaaaat aggggttacc 120tagataatgc caaagtcagc atttgtcctg ggttcccttg tgtgatctgt ttggactatg 180ttttcttttc ttctcccact tgctcagcag cttgggcttc cattctagct cttttaccaa 240gatttttgtg tgaccatgtt gacttcattt ggattgccct ctttcaattt ccttgtgaaa 300acacccttaa ctttctcttt acccttagct gaaatgttta cataacttct ggtgatatct 360tttcatgatt ttatatctct taaaatggtg atggatgtga cacctcataa aagtgagctt 420tgaactgtag ataactctta aagaaaatgt cattttanac aattaaaata tttgtgctca 480aaaaaaaaaa aaaaaaaaaa gtcctgcccg gcggccgtcn aan 523 189 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 189acaatttaat ttttctgctt gcccaagaaa caaagcttct gtggaaccat ggaagaagat 60gaaaatgaga ctggcaaaga acaaatgctg aatctgaaga agaggacaac tttgggcaaa 120taatctgcat acttttaatt gggaataaga tggaaaatat gaatgctaaa tcaaattttt 180taaaaaatac accacacgat acaactcaat acaggagtat ttcttctcaa attcttctag 240caccatcaac attcttcaag tatctgaaat actattaatt aagcaccttt gtattatgaa 300caaaacaaaa caaggacctc agttcatctc tgtctaggtc agcacctaac aatgtggatc 360acactcatgg gaaagtgttt tgaggtagtt taaacctttt ggaaggttgg gttttaaact 420tccctctgtg gaagatatca aaagccccaa gtggtgccaa atggttatgg ttttattttt 480caattttaat ttgggtttct tccaaaggtg acatttccat acaaggggaa gggggtggaa 540aaaaaatcaa attttggggg accagggagg ataatnaact gtttgcaatg cttgacaacc 600tttttttttt gnccaantaa ca 622 190 628 DNA Homo sapiens misc_feature (1)...(628) n = A,T,C or G 190accactaata gggtgtatct cagaaactga attgaaataa gggaaaatag gattttctgt 60cctggttttt gaagattgtt cttgattccc ttgattccca ggagagattc tctgacattc 120acgtgtcagc cactttggca cggaagcctt acagtgtggg gaaccaaaac ttcgtgtctc 180ctctttcccc gatgccatca gcatagactt gacttcctta aaccgagagt tttgatgtgg 240ccttggcaac cctaaaatca gctgtgttag gtaacaaaac tcaggctttc tgttgatgac 300atcgagatgg tgtcacttaa aagagccaag attcctgttt tcagtttgtg gattcatcct 360gctggtttta ctttagtccc tccatgtcaa agtgggcctg agaaaagctc atacatgcct 420catgtgaagt gtccaccccc tctgaaaatc tttcttgttc aaaacancna cgacatatct 480tggtaacttt tacggtgact tttggangag gggagtttgg aaattgtaaa atgttatana 540tggtgcctat ttcctgctga angaaatgtt ttaaaaagnn tntntaancn taatcnaatg 600gttggggggn gaccttctac cnaanntn 628 191 474 DNA Homo sapiens misc_feature (1)...(474) n = A,T,C or G 191ggtacagccc tcaatctgtt cttcaagctc aagaacttca agacagctgc cacctttgct 60cggcgcctac tagaactcgg gcccaagcct gaggtggccc aacagacccg aaaaatcctg 120tctgcctgtg agaagaatcc cacagatgcc taccagctca attatgacat gcacaacccc 180tttgacattt gtgctgcatc atatcggccc atctaccgtg gaaagccagt agaaaagtgt 240ccactcagtg gggcctgcta ttcccctgag ttcaaaggtc aaatctgcag ggtcaccaca 300gtgacagaga ttggcaaaga tgtgattggt ttaaggatca agtcctctgc agtttcgcta 360aagccccctt tgtgtgcatg ggtcaagtca ccatatgttc cccccaaaaa atgtgtctat 420atctccttct aacaacacct tcccctgcac tactcttcaa atctngctct ntgt 474 192 234 DNA Homo sapiens 192acgcgggggt tggtgagtgg gctcctaccg accgaggttt aggcagcgcg gggagctttg 60cgggttgcca tttgtaactc cggatcctaa aattcctgtc ctgttctctg tctcttctag 120gttgggggcc gtcccgctcc taaggcagga agatggtggc cgcaaagaag acgaaaaagt 180cgctggagtc gatcaactct aggctccaac tcgttatgaa aagtgggaag tacc 234 193 367 DNA Homo sapiens 193ggtaccaata ccaccaattt tgtagacatc ctggagaggc aggcgcaagg gcttgtcagt 60tggacgagtt ggtggtagga tgcagtccag agcctcaagc agcgtggttc cactggcatt 120gccatcctta cgggtgactt tccatccctt gaaccaaggc atgttagcac ttggctccag 180catgttgtca ccattccaac cagaaattgg cacaaatgct actgtgtcgg ggttgtagcc 240aattttctta atgtaagtgc tgacttcctt aacaatttcc tcatatctct tctggctgta 300gggtggctca gtggaatcca ttttgttaac accgacaatt agttgtttca cacccagtgt 360cccgcgt 367 194 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 194ggtactcttg gtttgtcaat gggactttcc agcaatccac ccaagagctc tttatcccca 60acatcactgt gaataatagt ggatcctata cgtgccaagc ccataactca gacactggcc 120tcaataggac cacagtcacg acgatcacag tctatgcaga gccacccaaa cccttcatca 180ccagcaacaa ctccaacccc gtggaggatg aggatgctgt agccttaacc tgtgaacctg 240agattcagaa cacaacctac ctgtggtggg taaataatca gagcctccgg tcagtcccag 300gctgcagctg tccaatgaca acaggaccct cactctactc antgtcacaa ggaatgatgt 360aggaccctat gagtgtggaa tccanaacga attaagtgtt gccacagcga cccagtcatt 420ctgaatgtcc tctatgncca gacgaacccc catttcccct cataccctan taccgtcaag 480ggtgaacctt agctttctgc atgcagcttt aaccactgcc agtttcttgn tgatgatgga 540catcacacca cacaagactn ttatttcaca tactgagaan aaagcgactt ntactgcagg 600cataactanc ngg 613 195 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 195acgcgggcgc cagagtccct gaactctcgc tttcttttta atcccctgca tcggatcacc 60ggcgtgcccc accatgtcag acgcagccgt agacaccagc tccgaaatca ccaccaagga 120cttaaaggag aagaaggtga tggtgaggaa gaggatggag atgaagatga ggaagctgag 180tcagctacgg gcaagcgggc agctgaagat gatgaggatg acgatgtcga taccaagaag 240cagaagaccg acgaggatga ctagacagca aaaaaggaaa agttaaacta aaaaaaaaaa 300aggccgccgt gacctattca cccttcactt tccgtctnaa aatctaaacg tggtcacctt 360caataaaaag gccccccgcc cccngggcag tgccccccca aaataaacgc gctttcacca 420ccaaccaaac atgaaaattt tccacaaggg anggaaaaaa aaccaaacnt ccaaggcctn 480ttttttttta aaatactngg ccgcgaccac cctanggcga attccanacc tggcggccgt 540nttatggatc cnactcggac caacttgggn aatatggcat antggttctt ggngaaatgt 600atccctccat tcn 613 196 296 DNA Homo sapiens misc_feature (1)...(296) n = A,T,C or G 196gcggnggcnn ggccgacgnn ctcatcaatg ttgttcggtc agcccttccc taattacacc 60tatccnctac acatacatgc acatagacac acncntgaac ncactgaana tatttccttc 120aggtgtgtgt aaaatatgct gcttggattg aaattcannt gggattgatt agncaagtan 180cttganacct cacagtaatc ttcacacttn nccttacaca cctatgcagg catgttggga 240gcangttaca atgttacttc agcccacagt ttatttctat acttgagttc ttaagt 296 197 222 DNA Homo sapiens 197acatggagga gaatgaccag ctcaagaagg gagctgctgt tgacggaggc aagttggatg 60tcgggaatgc tgaggtgaag ttggaggaag agaacaggag cctgaaggct gacctgcaga 120agctaaagga cgagctggcc agcactaagc aaaaactaga gaaagctgaa aaccaggttc 180tggccatgcg gaagcagtct gagggcctca ccaaggagta cc 222 198 539 DNA Homo sapiens 198cgaggtacta catatttcag cactaaggcg gttgcttcac tttatatcta tataaaaaaa 60gtggtaaaaa tcttttcctt ttgtgcagtt gaacccatcc tacattcaga ttctctcaag 120cactaataaa atacttattt ggttgaggaa gatttaaggc aagttcgggc ccttccaaag 180gcactgtgag actccccccc cactccccgt tattgctaca tgtctttata ctcgagtatg 240tcacagtaga actggtggaa taagcaaaca cttttttgct agtttataaa gttggaatta 300gaaaagcatg ccacatttca gcctgattgc aaagtatgtg gtcatttttt tctttgaagt 360tggatgggct acaaccttta tacattctaa gaaaactcat aggatgttcc tcaaactact 420tccacagcat caagatcgat ttctgtcaag aaatcatgca atctttcaaa atttacgtaa 480acaaggaaag aaattaatga aataaatatt acatacaatc tcttaaatta agaatttgt 539 199 626 DNA Homo sapiens misc_feature (1)...(626) n = A,T,C or G 199cgaggtacaa gatgtccaaa tattgcgaag atctatttgg ggatctcctg ttgaaacaag 60cacttgaatc acatccactt gaaccaggca gggctttgcc atcccccaat gacctcaaaa 120gaaaaatact cataaaaaac aagcggctga aacctgaagt tgaaaaaaaa cagctggaag 180ctttgagaag catgatggaa gctggagaat ctgcctcccc agcaaacatc ttagaggacg 240ataatgaaga ggagatcgaa agtgctgacc aagaggagga agctcacccc gaattcaaat 300ttggaaatga actttctgct gatgacttgg gtcacaagga agctgttgca aatagcgtca 360agaaggcttc agatgacctt gaacatgaaa acaacaaaaa gggcctggtc actgtagaag 420atgagcaggc gtggatggca tcttataaat atgtaggtgc tccactaata tccatncata 480tttgtccaca atgatcaact acgcccacct gtaaaggttc aaggttncat gtggcagaag 540aaccncatat tcattataca tggcttcttt tatgaatant cggccttggt tcttgaancc 600cttgcaatga atttgnaatt ntacca 626 200 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 200actcataaaa aaagtcttac cccaaaattg caaacaaata cattaaaaga ttagaagagg 60tgacagaaag caccagacat taaacaaaat aaaaataata aaataaattc aactcaaaag 120gtccccattc agcaaatact ttgtaaagta tggcctgtat gtaaatagtg ctaaatcaag 180gactttttag cagaaaattg ctcggttctt ttatctaagg cttgaatttg taaagtgaag 240gcataaaagt taccaaacat taagtaactc ttaaaatggc acacaggttt taaagctatt 300ggtttttcct tcctaactct ctgaattttt cccatggcct ttgtagatca actatttcaa 360acgtatttta caccagcaac tctcaacata cttgtctttc agatatgtca tcagtcatgt 420ctaacaggca aatagcanaa taacagattt aaaacaatcc ttaactanct agcaggacat 480ttactttgga ttctgcataa ctgcaaactg acatatttgt aaagctaaaa atcagtttaa 540tcntgattac agaaactcta tcatgctcat tacttaacta ttgnccttca atcgctattn 600aaattcactt aatccaat 618 201 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C or G 201ggtactaggc acaatagaac atacagaaaa cattgtccct gctcttgagg agcttacatt 60ctaaaagaaa aaatacacct tttttaaaat ggcatttttg tttggtgttt tctgcaaagt 120acgcggggct ttttcttttt gaggaagacg cggtcgtaag ggctgaggat ttttggtccg 180cacgctcctg ctcctgactc accgctgttc gctctcgccg aggaacaagt cggtcaggaa 240gcccgcncgc aacagccatg gcttttaagg ataccggaaa aacacccgtg gagtcggagg 300tggcaattca ccgaattcga atcaccctaa caagccgcan cgtaaaatcc ttggaaaagg 360tgtgtgctga cttgataaga ggcncanaag aaaagaatct canagtgaaa ggaccaagtt 420ngaatgccta ccaagacttt gagaatnact acgaganaaa ctccttgtgg tgaaggtcta 480agacgtgggn tngnttccag atgagaattc acaagcgact tattgacttc acaagtcctt 540ntgagattgt tangctgatt acttccttna ntatganccn ngaatttaag ngggangtna 600ccntncagan gnttagttna ctatttt 627 202 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 202actgcttaac gaaacactat cagcttgttt taaatggatc ttttaaatat caactgtagc 60ctggttggct aattctttct aatcttcccc attactttcg cctagatttc ccatagatca 120acaggcatag taaaatgcct catcagaaca cacttctcca cacaattcaa aaagggagct 180cctgtgggct caaagcaacc atcagtccag caatgcccat gatttatctg aaactgcttc 240ccaagagaca ggagtgcaga tctgagtagc tgtgctgcca atacagatag gtttagcact 300agatatttag tgattgtggc aaggaagaat cggtgatgat gggggtggtg ggtgaaggaa 360gggccagggg atctgaagga tcttcagttg ccttctcctg cttcttcatc ctgctggtcg 420ctcgtccana gggtgaggtt gtctcgcagc aactgcatga tcagcgtgga gtccttatag 480gaatcctcgt ttagtgtgtc cagctcagct atggcatcat cgaaggcttg tttggctaaa 540agcangcttg ctcangtgca ttctggatct catagtagaa caccggagaa ntganggcca 600ggcccaaccg gatnggatgc 620 203 577 DNA Homo sapiens misc_feature (1)...(577) n = A,T,C or G 203ggtacttttt tttttttttt tttttttttt tttttttttt tttttttttt tgaaaaagtc 60atggaggcca tggggttggc ttgaaaccag ctttgggggg ttcgattcct tccttttttg 120tctaaatttt atgtatacgg gttcttcnaa tgtgtggtag ggtggggggc atccatatag 180tcactccagg tttatggagg gttcttctac tattaggact tttcgcttcn aagcgaaggc 240ttctcaaatc atgaaaatta ttaatattac tgctgttaga naaatgaatg ancctacaga 300tgataggatg tttcatgtgg ggtatgcatc ggggtantcc gagtaacgtc ggggcattcc 360ggataggccn agaaagtgtt ntgggaanaa agttagattt accccgatga atatgatagt 420gaaatggatt ttggcgtagg tttggtctag ggtgtancct gagaataggg gaaatccgtg 480aatgaaacct cctatgatgg caaatacact cctattgnta ggacataatg ngaagtgagc 540tacaaccgta atacctgccc nggcnggccc ttannan 577 204 629 DNA Homo sapiens misc_feature (1)...(629) n = A,T,C or G 204cgaggtactt gttttttttt ttttttttga gacggagtct cagtctgtca cccaggctag 60agtgcagtgg cacgacatcg gctcactgca acctccgcct cccgggttca agtgattctc 120ctgcctcaac ctcccgagta gctgggacta caggcatgtg ccaccacgcc tgactaattt 180ttgtattttt agtanagatg ggatttcatt atgttggcca gctggtcttg aacttctgag 240ctcaggtgat ccacccgcct tagcctncca gagtgctagg ataacaggca tgagccgtcg 300cgcctggcca aaatagcata atgttttaag aaagtttacg aatttgtctt gggccacatt 360naaaaccatc atgggccaag ggttggacaa gctagcctta ggtcatgtca gaatgcaatt 420taacaggaat ttcaagcnaa acttacaaaa aattaaatcc acaaaaaaaa tatcatttgg 480taaatgcact gnctacacac tttactncta agtccattca accatgacga ccctttacat 540aaaaattagg gcattctccc aagttctaaa gatgatttct aaaacattac caangnctaa 600agtctaattc ccacaaanca ttttttttn 629 205 424 DNA Homo sapiens misc_feature (1)...(424) n = A,T,C or G 205ggtacaaatg cttttatatt cagcccctgt aaagccatca gatgtttgaa agtttttaaa 60cacgaaccaa agggtttaat tttaagaact tagctaggaa tgggtgaaat cctacccaat 120taatagagtt ctgcaaatta gtaacaaagt gtaaaatgaa aggaagggtc ccttggagat 180gtgaaattct tctattgaga gtcctgtctt ctttattcaa gaagtttgta gccattttca 240gaattcactc aagaaccaac ttcttaattt agatatcagc gaacaagtca tggcaaaaaa 300tacacaaaga gaaacaccac cacatcgaaa aggatgaaaa gccagaggtc caaccagtan 360gagtgtttgg gaagcccatt tgccccagac tgaggcctca catcgaagtt ctgcctcccc 420gcgt 424 206 633 DNA Homo sapiens misc_feature (1)...(633) n = A,T,C or G 206ggtaccaatg gtgcctcctg gaatcaagta tctttacctt aggaataacc agattgacca 60tattgatgaa aaggcctttg agaatgtaac tgatctgcag tggctcattc tagatcacaa 120ccttctagaa aactccaaga taaaagggag agttttctct aaattgaaac aactgaagaa 180gctgcatata aaccacaaca acctgacaga gtctgtgggc ccacttccca aatctctgga 240ggatctgcag cttactcata acaagatcac aaagctgggc tcttttgaag gattggtaaa 300cctgaccttc atccatctcc agcacaatcg gctgaaagag gatgctgttt cagctgcttt 360taaaggtctt aaatcactcg aataccttga cttgagcttc aatcagatag ccagactgcc 420ttctggtctc cctgtctctc ttctaactct ctacttagac aacaataaga tcagcaacat 480ccctgatgaa gtatttcaag cgtttaatgc tttgcagtat ctgcgtttat ctcacaacga 540actggctgat agtggaatac ctggaaattc tttcaatggn gccatcctgg gtgaacctgg 600acttgcctat accagcntaa aacataccac cgg 633 207 623 DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G 207ggtacttttt tttttttttt tttttttttt ttagaaacta tggctcttta ttttcatgtg 60gataattcaa acaaagtcat tagtagtctt tgttcaattt ttttttaaaa aacaaaaaaa 120ccctcaaata aaaaatcttg ggcttaaaag aactctatca caggagcctg gttggaggat 180tcctagtttt atacatgaga aatagaatgc agatttctct gaagagtgtt taaagaagga 240atggtagttg agggggctta tttcccaggc tcaaagtgat ttaggggtgg tgtcacagtg 300ctaggtatag ggtgatggac agtgatcact gccgagggcc ttggaacgga tcttgctgtc 360acacaatgca ggtaacagag agtgggacaa caaaaagtaa tcaaggcgcc aaccaacatt 420cttggatcga gcattcatat ataagtccaa aaggtgtang cataaggtgt gttggggtan 480aagtgcctaa agctgcaacc agtggcacan cctgcagtaa ttccccgaac cttggccttt 540tggggcgtga anccnccatt cttttggtnc cctnggggtg cnaaggcaat ttttnatgtg 600cccattgagg gttcaaacac aca 623 208 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 208acgatgtcta gtgatgagtt tgctaataca atgccagtca ggccacctac ggtgaaaaga 60aagatgaatc ctagggctca gagcactgca gcagatcatt tcatattgct tccgtggagt 120gtggcgagtc agctaaatac tttgacgccg gtggggatag cgatgattat ggtagcggag 180gtgaaatatg ccccgcgtac ttgctttgaa agattaccta ctattttatg ataaaatgta 240gttgtctcca gagcttaaat ataatttgta aagcacttgg tttaaatttc tctctaccta 300taaacagttt agcattaagg gtttctatta atgacacaga attattggcc aagtgtaatt 360tcttaaaatt tagcattact ttaaatagcc agcatgtaat acaagtaact acactacctc 420atatctacat gattttcaag ttgtaatgca gatggacaga taaaaaagat ttacgttgnc 480ttttggccat aagtgggaaa agttttctgn atattgcata gcattacaca tttatgccta 540ttttacatta acttctaaag aagtttttct aagaaaangg ttcaggcaat attttttgag 600gctgccgaan aaaaatgant 620 209 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,C or G 209ggtactggta caaaaacagg cacataaacc aatgaaacag aatagaaagc ccagaaataa 60tgcttcaccc ccacaaccat ctgatcttca acaaaataaa caaaaacgag ccatggggaa 120aggactccct attcaataaa tggtgctggg ataactagtt aaccatatgc agaagattaa 180agctggaccc cttccttaca aaataaggag ctggacccct tatacaaaaa tcaactcaag 240atggattaaa gccttaaatg tgaaactata aaaccctgga agacaacata ggcgattcca 300ttctagacat cagaactggc aaagatttca tgaggaagac accaaaagca attgcaacaa 360aagcaaaaat tgacaactgg gatataatta agtttaagag cttctgcaca gcaaaagaga 420gactatcagc agagtaaaca gaccacctac agaatgggag aaaatatttg caaactatgc 480atgtgacaaa ggtctaatat ctagcatcta taagtactta aacaaatttc aacagaaaac 540caacacccca ttaaaaagtg ggcaaggaca tgaacaaatg cctttcaaaa gaagacatct 600gcttntacag tttntgaaac aaag 624 210 504 DNA Homo sapiens 210acgcggggca gctagcagat gctttaggac ctagtatctg catgctgaag actcatgtag 60atattttgaa tgattttact ctggatgtga tgaaggagtt gataactctg gcaaaatgcc 120atgagttctt gatatttgaa gaccggaagt ttgcagatat aggaaacaca gtgaaaaagc 180agtatgaagg aggtatcttt aaaatagctt cctgggcaga tctagtaaat gctcacgtgg 240tgccaggctc aggagttgtg aaaggcctgc aagaagtggg cctgcctttg catcgggggt 300gcctccttat tgcggaaatg agctccaccg gctccctggc cactggggac tacactagag 360cagcggttag aatggctgag gagcactctg aatttgttgt tggttttatt tctggctccc 420gagtaagcat gaaaccagaa tttcttcact tgactccagg agttcagttg gaagcaggag 480gagataatct tggccaacag tacc 504 211 619 DNA Homo sapiens misc_feature (1)...(619) n = A,T,C or G 211accatgaaat atccagaaca tacttatatg taaagtatta tttatttgaa tccacaaaaa 60acaacaaata atttttaaat ataaggattt tcctagatat tgcacgggag aatatacaaa 120tagcaaaatt gaggccaagg gccaagagaa tatccgaact ttaatttcag gaattgaatg 180ggtttgctag aatgtgatat ttgaagcatc acataaaaat gatgggacaa taaattttgc 240cataaagtca aatttagctg gaaatcctgg atttttttct gttaaatctg gcaaccctag 300tctgctagcc aggatccaca agtccttgtt ccactgtgcc ttggtttctc ctttatttct 360aagtggaaaa agtattagcc accatcttac ctcacagtga tgttgtgagg acatgtggaa 420gcactttaag ttttttcatc ataacataaa ttattttcaa gtgtaactta ttaacctatt 480tattatttat gnatttattt aagcatcaaa tatttgtgca agaatttgga aaaatagaag 540atgaatcatt gattgaatag tattaagatg tatagtaaat tatttatttt ananattaaa 600ngangtttat taganaaan 619 212 479 DNA Homo sapiens 212cgaggtacaa agcagcaact gcaatactca aggttaaaac attagaaaag catttgtgtg 60acaggtatat tacagtatta tcaaaatatt acattttcag acttacttag cagataatca 120tccaccagag cttaaatctt taaattattt ccatagtctt aaaaaatatg taatgtcaga 180atgcatataa aaagaatgta aaaggaaacc taaaatacaa atggaataat gtaacaaata 240aatatttgat ttcagtaact gttaataatc agctcaacac caccattctc tctaaactca 300atttaattct tataggaata atgaactgtc aaatgccatg gcataattat ttatttccaa 360gctatcatca atgattagaa ctaaaaaaat tttggcataa aaaaatcaca attcagcata 420aataaagcta tttttagctt caacactagc tagcatctct aagaattgtt gaaataagt 479 213 487 DNA Homo sapiens 213actgtttact gcctgggcac tatactttct atgcagatct cctttgtggg tttccagcct 60gtcctttcat cagagcacat ggcagccttt ggggtctttg gtctctgcca gatccatgcc 120tttgtggatt acctgcgcag caagttgaat ccacaacaat ttgaagttct tttccggagc 180gtcatctctc tggtaggctt tgtccttctc accgtgggag ctctcctcat gctgacagga 240aaaatatctc cctggacggg gcgtttctac tcactgctgg atccctctta tgctaagaac 300aacatcccca tcattgcttc tgtgtctgag catcagccca caacctggtc ctcatactat 360tttgacctgc agctcctcgt cttcatgttt ccagttggcc tctattactg ctttagcaac 420ctgtctgatg cccggatttt tatcatcatg tatggtgtga ccagcatgta cctcggccgc 480gacacgc 487 214 393 DNA Homo sapiens 214cgaggtacaa tatgctgcag cataatttgt caggccaacc ttcacaccat attttggcag 60ttcgtgtgca tacgctgcgc agactatcat atccccctct atacgggcat aagcaatctg 120acaaatgata tctctgtttg tcacacgaac tatcatcctg tatttgggtg tgttgtattt 180atttttatct tgtatcacca agcgtttccg agcataataa tcagttttac cctctcgtcg 240tcttctaaat ttcacttggt atctcttaaa gtaggcctta ttcttaacaa ctttaacaaa 300ccccatcctg cggaacagag accggcgtcc gctgctcgac agagacctgc aggcccagcg 360gcgctagggg gtgggaaaag ggccaccccc cgt 393 215 615 DNA Homo sapiens misc_feature (1)...(615) n = A,T,C or G 215ggtacagtaa caagtgttgg cattatcagt tgaactgtaa atacaaaatg cttcttccaa 60ttagtctcta tgatgattaa gtttctaaaa tttatctgaa caccattcag aaacttgttt 120tggggaattt gatagttatt gatgtgcatc tgttaaactg atgacagaca taactcatca 180ttccccagaa accttttttg attacagtat ctaacatttt gcctcctctt ttttggtttt 240gctggttata aaggtttgga ttggagaggg ctcactggat cccaatcctt ggagctggat 300cattggattc aaatcataat gtggatagga tagggaggat gaattaccag gattcatgga 360gcgggatcag attaccagga acataggagt ggattcctgc ccaaccaaac ccgcattcgt 420gtggattttt ttattcaact taattggcta ttccaaagat ttttttttcc tatttttgac 480gaatggagcc cttaagatgc acgatggaat tgggtttgcg tttttggtaa aaggaccaaa 540ccaggcctgg agataacgct ggagcaatct cntggaagga ttagccccaa ttgatgggaa 600catttaangg ggaag 615 216 322 DNA Homo sapiens misc_feature (1)...(322) n = A,T,C or G 216ggtacttttt tttttttttt tttttttttt ttttttggag ttgtaggcaa atgtttaatt 60aattctgctc atatgcacat ctgaaagcat gagacacact ccacagacag cacgcactgg 120ggctggtggg gcanatgggc actcgccgat taggtattaa tgtcaataat acgtgcataa 180agtgctgata aaataactta agtgttacaa aaagagacag tccacggtgg ctgcaggcac 240atgcaggcgg gactgggtca aacactccag ggctgcacat gttccagctg gcctgagtcc 300gacacgtcat aactggcctt gt 322 217 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 217acgcgggggg aagtgagcga cacactctgc gtcctcgcct caccagagtc ttgctgtgtg 60gcccaggctg gagtgcccgg ctggtctcaa attcctgacc tcaagtgatc tccctcccaa 120agtgttgcga ttgcaggtgt gagccactgc acctggctgc tgagaaatct ttgcctacag 180tgagggaaac tactaaagtt cctggggaag caaagtaaga atttcataag aacaaaatgg 240atggagagga gaaaacctat ggtggctgtg aaggacctga tgccatgtat gtcaaattga 300tatcatctga tggccatgaa tttattgtaa aaagagaaca tgcattaaca tcaggcacga 360taaaagccat gttgagtggc ccaagtcaat ttgctganaa cgaaaccaat gaggncaatt 420ttagagagat ccttcacatg tgctatcgaa agtattcatg nattttacgt accttgggcc 480gcgaccacct taaggccaat tncacacact ggcnggccgt actantggat ccnactngga 540ccaacttggc gtaatcatgg catactggtt cctggggaaa atgtatccgt tacaattcnc 600acacan 606 218 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 218ggtacttttt tttttttttt ttttttttga gacggagttt ggcccttgtt gcccaggctg 60aagtgcaata gtgcgatctc ggctcactgc aacctccacc ttccgtgttc aaccgattct 120cctgcctcag cctcctgagt agctgggatt acagatgaaa aaacatttaa agcccttaag 180gaagaaggaa atcaatgtgt aaatgacaaa aactataaag acgccctcag taaatacagc 240gaatgcttaa agattaacaa taaggaatgt gccatatata caaacagagc tctctgttac 300ttgaagctgt gccagtttga agaagcaaag caggactgtg atcaggcact tcagctagct 360gatgggaacg tgaaagcctt ctatagacga actctggctc ataaaggact caagaattat 420cagaaaagct taattgatct caataaagtt atcctactag atccaagtat tattgaggca 480aagatggaac tggaagangt aactagactc ctaatcttaa ggataagaca gcaccattca 540acaaagaaaa ggagagaagg aaaatgagaa tcaagaggng aatgaaggca ngaggancct 600ggaaaacctg aggggagg 618 219 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 219ggtacaaagc ggatctgagc ccggaaaatg ctaagctcct cagcacattc ctaaatcaga 60ctggcctaga cgccttcctg ctagagctgc acgaaatgat aatcttgaaa ctaaagaacc 120cccaaaccca aaccgaggag cgcttccgcc ctcagtggag cctgagagac actctcgtaa 180gttacatgca aactaaagaa agtgaaattc ttcctgaaat ggtatctcag ttcccagaag 240agatactgct cgccagctgt gtctcagtgt ggaaaacagc tgctgtgctg aaatggaatc 300gagaaatgag atagaattat ttcctcagct atctttggat gactttggag agaagactcc 360tctctcctcg tctgcggcgt ggacttgatc atggactggt gcctttgcat tcagaaggag 420agctgtcagc gtagcaccga attcaagacc aaggcgtgct acctgagctg acagcttttt 480gaaagccgag ctggttctga accatgtcct gcccnggcng gcgctcgaaa gggcgaattc 540agccactggc ggccgtacta ntggatccga actcggacca aacttggcgt aatatgggca 600tactggttcc tgg 613 220 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 220ggtacgcggg ggcagccgcg gtgttgtgct gtggggaagg gagaaggatt tgtaaacccc 60ggagcgaggt tctgcttacc cgaggccgct gctgtgcgga gacccccggg tgaagccacc 120gtcatcatgt ctgaccagga ggcaaaacct tcaactgagg acttggggga taagaaggaa 180ggtgaatata ttaaactcaa agtcattgga caggatagca gtgagattca cttcaaagtg 240aaaatgacaa cacatctcaa gaaactcaaa gaatcatact gtcaaagaca gggtgttcca 300atgaattcac tcaggtttct ctttgagggt cagagaattg ctgataatca tactccaaaa 360gaactgggaa tggaggaaga agatgtgatt gaaagtttat cangaacaaa ccgggggtca 420ttcaacagtt tanatattct ttttaatnnt ttcttttncc tcaatccttt tttattttta 480aaaatagttc ttttgtaatg tggtgtcaaa acggaattga aaactggcac cccatctttt 540gaaacatctg gtaatttgaa tctaatgctc attatcatta tggttggttt cattggcnga 600attttgggga tcaanc 616 221 615 DNA Homo sapiens misc_feature (1)...(615) n = A,T,C or G 221ggtacagtga tagctccccc tgggcaatac aatacaagaa cagtgggttt tgtcaaattg 60gaacaaggaa acagaaccac agaaataaat acattggtta acatcagatt agttcaggtt 120acttttttgt aaaagttaaa gtagagggga cttctgtatt atgctaactc aagtagactg 180gaatctcctg tgttcttttt tttttaaatt ggttttaatt ttttttaatt ggatctatct 240tcttccttaa catttcagtt ggagtatgta gcatttagca ccactggctc aatgcgctca 300cctaggtgag agtgtgacca aatcttaaag cattagtgct attatcagtt accaccattt 360ggggctttta tccttcatgg gttatgatgc tctcctgatg acacatttct ctgagttttg 420taattccagc caaagagaga ccattcacta tttgatggct ggctgcatgc agacatttaa 480agcttttaga gaatacacta caccagggag tatgactact antatgacta ttagganggt 540aatacccaga attggactcg caccttaggc aagatccaac cactaaattg aataagaatg 600agtngatgag gtncc 615 222 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 222ggtacttttt tttttttttt tttttttttt ttttaattta tgattttatt gnctttcctt 60tgtccggcct ttaacatgtt tctgtaattt aaataaaaat ctatttactt tctccatttt 120agcaaatggt ttctttaccc aaataggttg cactatagtc cccatatggt tttctactgn 180tccacaacca ctatttcaca aagattgaca aaactttaat aaaagttaaa tttacagaca 240tcttaagata acttgggaaa tatgtagtaa aaaagaatcg agtccacaaa ttaagaatat 300tttgctaata tgcccaacac caatttcagc aaatccaatc tacttaactc atatatttaa 360tgnggtaatt tttctaacaa aatttaatgg gggtatgaat gatatattta tgcccttgac 420aaagatgaca tgtgtgattt tggtgngact aanaaaggag aagtatgatt tctggngggt 480atganatcac tctggctcat cgaagctcca gaatatgtaa gggtctgnca cgtccaaaaa 540tgttaggcna atgtataaaa ggccacccgg ctnacacacg ttttatatac aaactttngn 600agtcctttta tntcata 617 223 470 DNA Homo sapiens misc_feature (1)...(470) n = A,T,C or G 223ggtaccacaa ctgtgccctt gataattagt aatcactcct aaaaatcttc atttggcacc 60agatggtgtg tttaaaacac cctaggatgt tttgaatcag gcttgatttt gttagttgag 120ttacaggaga attttaaggg tgagggtatg ggggtcaggg aagaaaagga aatgggaaat 180ggaccagaaa aaatcttgag tcatcatcta aatcaacaaa gcactgatag ctccaaatat 240taggtcagac actaaaacga ctgatatagg ctcaagtggt ttataaaacc tataaaaaga 300ctacaccagc aaagtccctg tcaatctgtc agagttcaga aactaaaaca gggagtaaca 360ttttagctta aaaccttatc tcaagagaat catatacact tcacatgaat aaaaatacct 420gaaaccaaac atttttaaaa gctccagtcc tgcccnggcc ggccgctcga 470 224 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 224gcgtggncgc ggccgacgtn ctcttttttt tttttttttt ttttttgcnn actaaaaatn 60ngattgctct ttaaagcctt aggccgnatg acaaaatgan nagactgaaa tgacancggg 120gaggaagaaa cagannaaag ataagaatga ggtggtcagg ttgggggaat taagcgaata 180ttcncttccn nggtgagtcc tnacactggt ctcatgccca tgatgagttg cacaccaaac 240acnggctgnt gacttncctc ctgcnctant cagtgaactt gcngacatng ggnancctca 300cattacagnt ataanntttc cacctaaaaa atgctgcgct tttcgacngg ctcnnncagn 360ggccggggct tgacatggng gaanggattt ctctcccatg ccaaggaatt catcacatca 420ctgntactcc actgncaacc ttntccattg ggctcngtgc cctgtgtngg gtcatggacc 480cantccanaa ntatgaatac tgtaccatgc tcttaaccag gaggacctaa ggatccttag 540ncccntgagn nanacaccag gnttcaaagg ccgttttggn aagccaaatt tgnttnggnc 600cgaattnggg ccaaacangg tt 622 225 619 DNA Homo sapiens misc_feature (1)...(619) n = A,T,C or G 225acgcggggag ttccgccatg gcctccttgg aagtcagtcg tagtcctcgc aggtctcggc 60gggagctgga agtgcgcagt ccacgacaga acaaatattc ggtgctttta cctacctaca 120acgagcgcga gaacctgccg ctcatcgtgt ggctgctggt gaaaagcttc tccgagagtg 180gaatcaacta tgaaattata atcatagatg atggaagccc agatggaaca agggatgttg 240ctgaacagtt ggagaagatc tatgggtcag acagaattct tctaagacca cgagagaaaa 300agttgggact aggaactgca tatattcatg gaatgaaaca tgccacagga aactacatca 360ttattatgga tgctgatctc tcacaccatc caaaatttat tcctgaattt attagcccgt 420ggggccaatt ttttaactca natcttgctg agaccaggag catctgattt aacaggaagt 480ttcagattat acccgaaaaa gaagttctag agaaattaat agaaaaatgt ggttctaaag 540gctacgtctt ncaaatggag atgattggtc nggcaagaca gttgaatatt ctattggcga 600ggttccatat canttgngg 619 226 277 DNA Homo sapiens 226acgcggggcc cctcatttac ataaatatta tactagcatt taccatctca cttctaggaa 60tactagtata tcgctcacac ctcatatcct ccctactatg cctagaagga ataatactat 120cgctgttcat tatagctact ctcataaccc tcaacaccca ctccctctta gccaatattg 180tgcctattgc catactagtc tttgccgcct gcgaagcagc ggggggccta gccctactag 240tctcaatctc caacacatat ggcctagact acgtacc 277 227 328 DNA Homo sapiens 227ggtacatatt tttgccaatg ctatacagca aaaatgaaaa acttacagaa aggtaaacaa 60aattgagtcc acttttttaa tttcacaagc tgctttaaac tatagaacca ccagatatct 120gtaaaataag caaaactggt aagtgtgttt ttttaattga gggaaggagg gccagaggag 180ttggtgcaga agcgcttcgg gtgaattcat accagagcca ccgggtgtga ctcggctacc 240tctcccaatt accacaggga ggtcttaaaa ttgaatttca gtttcagcag atactccaga 300tttacctgag caatatcata gacaatgt 328 228 609 DNA Homo sapiens misc_feature (1)...(609) n = A,T,C or G 228acgcgggagt tcaagcagat gtatggctaa ccggaaacag gtgggtcacc tcctgcaaga 60agtggggcct cgagctgtca gtcatcatgg tgctatcctc tgaacccctc agctgccact 120gcaacagtgg gcttaagggt gtctgagcag gagaggaaag ataagctctt cgtggtgccc 180acgatgctca ggtttggtaa cccgggagtg ttcccaggtg gccttagaaa gcaaagcttg 240taactggcaa gggatgatgt cagattcagc ccaaggttcc tcctctccta ccaagcagga 300ggccaggaac ttctttggac ttggaaggtg tgcggggact ggccgaggcc cctgcaccct 360gcgcatcagg actgcttcat cgtcttggct gagaaaggga aaagacacac aagtcgcgtg 420ggttggagaa gccagancca ttccacctcc cttccccaac atctctcana gatgtgaaac 480cagatctcat ggcaacnaag ccctntgcaa gaagctcaag gaanctaagg aaaatggacg 540ttttcagana atggttgtag ttcatgggtt ttncctactg ccgggtcctt tcttangacc 600cgcanaant 609 229 610 DNA Homo sapiens misc_feature (1)...(610) n = A,T,C or G 229ggtacttttt tttttttttt tttttttttt gcagactaaa aattttattg ctctttaaag 60ccttaggccg tatgacaaaa tgaagagact gaaatgacag cggggaggaa gaaacagaag 120aaagataaga atgaggtggt caggttgggg gaattaagcg aatattctct tccagggtga 180gtcctcacac tggtctcatg cccatgatga gttgcacacc aaacacaggc tgctgacttc 240cctcctgcac tagtcagtga acttgcagac atagggtaac ctcacattac agttataatc 300tttccacctc agaaatgctg tgcttctcga caggctcgca cagtggccgg ggcttganat 360ggtggaggga tttctctccc atgcaaagta attcatcaca tcactgntac tccactccca 420accttctcca ttgggctcgg tgccctgtgt ggggtcatgg acccaatcca acgtatgant 480actggtacca atgctnttac cagggaggac acnaaaggat cccttacccc ctgagcacag 540acccnaggtt tcaaanggcc gttttggcag gccaaactgn atntgnccag aatttggnga 600caaaacaagg 610 230 346 DNA Homo sapiens 230ggtcggccga ggtaccatgc actgagtgac tgtggggatc atgttgttat aatgaacaca 60agacacattg cattttctgg aaacaaatgg gaacaaaaag tatactcttc gcatactggc 120tacccaggtg gatttagaca agtaacagct gctcagcttc acctgaggga tccagtggca 180attgtaaaac tagctattta tggcatgctg ccaaaaaacc ttcacagaag aacaatgatg 240gaaaggttgc atctttttcc agatgagtat attccagaag atattcttaa gaatttagta 300gaggagcttc ctcaaccacg aaaaatacct aaacgtctag atgagt 346 231 601 DNA Homo sapiens misc_feature (1)...(601) n = A,T,C or G 231ggtacgcggg gagagcacat ccggtgttag aagcgctggt aggccttgga gaggcgggtt 60aggaagagtg gagactgctg cacggactct ggaaccatga acatatttga tcgaaagatc 120aactttgatg cgcttttaaa attttctcat ataaccccgt caacgcagca gcacctgaag 180aaggtctatg caagttttgc cctttgtatg tttgtggcgg ctgcaggggc ctatgtccat 240atggtcactc atttcattca ggctggcctg ctgtctgcct tgggctccct gatattgatg 300atttggctga tggcaacacc tcatagccat gaaactgaac agaaaagact gggacttctt 360gctggatttg cattccttac aggagttggc ctgggccctg cctggagttt tgnattgctg 420tcaaccccac atccttccac tgctttcatg ggcccgcaat gatctttacc tgcttaacct 480taatgcactc tatccaagcg ccgtactcct tttctgggag gatcttgatg tcagcctgaa 540cttgtgcttt gcttcctggg gaatgtttct ttggatccat tggcttttca gcnaactttt 600t 601 232 390 DNA Homo sapiens misc_feature (1)...(390) n = A,T,C or G 232actttttttt tttttttttt tttttttttt ttggttttaa tgtttatttc cccaagacag 60cctagcctgc actctacttg gataaatttt acaagctagt tttctgctgc ttctagtttt 120aaactttaac catgtttctg atgacaagga atgctgcaaa aatactctag ttcaacaaag 180agttatgatc acaaaataat ttttatccat tctacagtgt ttcanaatta ccagttgatt 240tttaaacaca aagtagatat agatgctaat ggtggctaat ctggtatgtt tcttatagca 300aactgttgtt catgcaacac ttgtgctcaa aggggaaggc acaggatttc ctacaatgag 360ccaccttata aagagttctt tttgnacctn 390 233 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,C or G 233cgaggtacgc gggggaagag tgagggttcc aacttttctg cttatctggg aggtgttggg 60cgcggacaat cgagatgtca gagaaaaagc agccggtaga cttaggtctg ttagaggaag 120acgacgagtt tgaagagttc cctgccgaag actgggctgg cttagatgaa gatgaagatg 180cacatgtctg ggaggataat tgggatgatg acaatgtaga ggatgacttc tctaatcagt 240tacgagctga actagagaaa catggttata agatggagac ttcatagcat ccagaagaag 300tgttgaagta acctaaactt gacctgctta atacattcta gggcagagaa cccaggatgg 360gacactaaaa aaatgtgttt atttcattat ctgcttggat ttatttgtgt ttttgtaaca 420caaaaaataa atggtttgat ataagaaaaa annnnnnnna aaaaaaaagt nctggccngg 480cggccgttca aanggccaat tccacccact ggcggccgta ctaanggacc aacttggncc 540aacttgggga atcanggcaa actggttcct ggngaaatgg nttcccttcc aattccccaa 600atn 603 234 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 234cgaggtacct tcattgcgat caaaccagat ggggtccagc ggggtcttgt gggagagatt 60atcaagcgtt ttgagcagaa aggattccgc cttgttggtc tgaaattcat gcaagcttcc 120gaagatcttc tcaaggaaca ctacgttgac ctgaaggacc gtccattctt tgccggcctg 180gtgaaataca tgcactcagg gccggtagtt gccatggtct gggaggggct gaatgtggtg 240aagacgggcc gagtcatgct cggggagacc aaccctgcag actccaagcc tgggaccatc 300cgtggagact tctgcataca agttggcagg aacattatac atggcagtga ttctgtggag 360agtgcagaga aggagatcgg cttgtggttt caccctgagg aactggtaga ttacacgaac 420tgtgctcana actggatcta tgaatgacag gaaggcagac ccattgnttt tcacatncat 480ttcccttcnt tccattgggc aaaggaccag ctttnggaaa tctantnttt accnggacct 540tattcttaat ttgganggaa actnttggac tttgangtnt tcctntacct ngcccgggng 600gccgtttaaa agggna 616 235 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 235acgcggggag tgcgttactt acctcgactc ttagcttgtc ggggacggta accgggaccc 60ggtgtctgct cctgtcgcct tcgcctccta atccctagcc actatgcgtg agtgcatctc 120catccacgtt ggccaggctg gtgtccagat tggcaatgcc tgctgggagc tctactgcct 180ggaacacggc atccagcccg atggccagat gccaagtgac aagaccattg ggggaggaga 240tgactccttc aacaccttct tcagtgagac gggcgctggc aagcacgtgc cccgggctgt 300gtttgtagac ttggaaccca cagtcattga tgaagttcgc actggcacct accgccagct 360cttcaccctg agcagctcat cacaggcaag gaagatgctg ccaataacta tgcccgangg 420cactacacca ttggcaagga gatcattgac cttgngttgg acccaattcc aaacctggct 480gaccatgcac cgggctttan ggnttnttgg gttttcccaa antttggggg ggaactgggt 540ttgggttaac ttcctgntna tggnacgntt ttaaatgaat ntgggaaaaa tccaactggn 600gntttcc 607 236 608 DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 236acgcgggcat gcaacaccac acccagcctg aaacccagat ttttaatatg aaatcaaagt 60cttcagacct tgtaggtgtc ataaaaagca cgctgaggac cactagtttg caactgccaa 120tctaaaatat catagacatt atatcacttc aaccacgaaa aaaaagtatg tgaggcagaa 180aatggaagca accatgccta atttattgtt gaatactttt tccgtatacc aagagcttcc 240tttgcactag catctgaaac tatatccaga atgacactgg ttttcataaa agtgttgatc 300ctcacacctc tttatagtct tgcacctagc acagtggagt gaaacacttt aaatagcact 360tgntccttga gtatatatgg aaaaaagtga agtattgata aagtgctcaa ctaatatgag 420cagcatctca ggagtctcca attcttgaat taccagggag tatttttacc attttcccca 480ntgnaaggcc ttttttgaga nacttaccct caaatngaan gnnttaagca tgntcctttt 540tttttccttt tttttttgan aaaagggctt gctntgtggc caggttggan tgcctacntg 600aaaattcn 608 237 609 DNA Homo sapiens misc_feature (1)...(609) n = A,T,C or G 237actatttcat atattgtgtg agccccacaa atgtctattt taaaaagagt atagtccctg 60gccaggcgcg gtggctcacg cctgtaatcc cagcagtttg ggaggccgag gtgggcggat 120cacctgaggt ctggagttcg agaccagcct gaccaatatg gtgaaacccc gtttctacta 180aaaatacaaa attagctggg catggtggag catgcctgta atcccagcta ctcgggaggc 240tgaggcagga gaatcacttg aacccgggag gcgaaggctg cagtgagcca agatcacgcc 300attgcactcc agcctgagca acaagaggga cactccgtcc ccaaaaaaaa aataataaaa 360aaaataaaaa ataaaaataa aaagagtata gttcccaatg ggttctacaa acattcctga 420tttatactgg gggaagtgat gcctaantgg gaacattaat cattatggtt tcgaaaatta 480aatatttctg caaacaattc ctttgcaaat gctaacttgc catgagctta ccccatttga 540aattgngnct ttacaaagac cttggccgga ccccttangg ngaattcagn cactggnggg 600cgttctttg 609 238 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 238acgaggcggt gcgggaagtc ctgcacggga accagcgcaa gcgccgcaag ttcctggaga 60cggtggagtt gcagatcagc ttgaagaact atgatcccca gaaggacaag cgcttctcgg 120gcaccgtcag gcttaagtcc actccccgcc ctaagttctc tgtgtgtgtc ctgggggacc 180agcagcactg tgacgaggct aaggccgtgg atatccccca catggacatc gaggcgctga 240aaaaactcaa caagaataaa aaactggtca agaagctggc caagaagtat gatgcgtttt 300tggcctcaga gtctctgatc aagcagattc cacgaatcct cggcccaggt ttaaataagg 360caggaaaagt tcccttcctg ctcacacaca acgaaaacat ggtggccaaa agtggatgag 420gtgaagtcca caatcaagtt ccaatgaaga aggggtatgt ctggcttgta acttgttggt 480cacgtgaaga tgacngacga tgacttgngt ataacattna nctgggctgg caacttcttg 540gggcaatgnt caanaaaact ggcaaaatgt ccgggccttt tttttagagc cccttggnaa 600accccangcc ntttta 616 239 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 239acagtctgtt cgagaacacc ttggtcatga aagtgacaac ctgctgtttg ttcagatcac 60aggcaaaaaa ccaaactttg aagtgggttc ttctaggcag cttaagcttt ccatcaccaa 120gaagtcttct ccttcagtga aacctgctgt ggaccctgct gctgccaagc tgtggaccct 180ctcagccaac gatatggagg acgacagcat ggatctcatt gactcagatg agctgctgga 240tccagaagat ttgaagaagc cagatccagc ttccctgcgg gctgcttctt gtggggaaag 300ggaaaaagag gaaggcctgt aagaactgca cctgtggcct tgccgaagaa ctggaaaaag 360agaagtcaag ggaacagatg aacttccaac ccaagtcaac ttgtggaaac tgctcctggg 420cgatgccttt cgttgtgcca ctggccctac cttgggatgc cagcntnaaa ctggggaaaa 480gngcttctaa tgatancatc tttattgaag cctaagaagg ttctgaattg ggacccattt 540gttcttcaac caattctggn cttaaatcca ccttgggggt cttccacctc cttggatttg 600ncacctt 607 240 615 DNA Homo sapiens misc_feature (1)...(615) n = A,T,C or G 240ggtacgcggg gcttttcaca agatggcgcc gaaagcgaag aaggaagctc ctgcccctcc 60taaagctgaa gccaaagcga aggctttaaa ggccaagaag gcagtgttga aaggtgtcca 120cagccacaaa aagaagaaga tccgcacgtc acccaccttc cggcggccga agacactgcg 180actccggaga cagcccaaat atcctcggaa gagcgctccc aggagaaaca agcttgacca 240ctatgctatc atcaagtttc cgctgaccac tgagtctgcc atgaagaaga tagaagacaa 300caacacactt gtgttcattg tggatgttaa agccaacaag caccagatta aacaggctgt 360gaagaactgt atgacattga tgtggccaag gtcaacaccc tgattcggcc tgatggagag 420aagaaggcat atgttcgact ggctcctgat tacnatgctt tggatgttgc caccaaaatt 480gggatcattt aactgagtcc acttgctaaa tctgaatata tatatatata tatatctttt 540cnccccaaaa aaaaaaaaaa aaaaaagtnc tncccggcgg ccgtttaaag gggaattccc 600cacttggggg cgttt 615 241 365 DNA Homo sapiens misc_feature (1)...(365) n = A,T,C or G 241acgggggggt cgctttgctg ttcgtgatat gagacagaca gttgcggtgg gtgtcatcaa 60agcagtggac aagaaggctg ctggagctgg caaggtcacc aagtctgccc agaaagctca 120gaaggctaaa tgaatattat ccctaatacc tgccacccca ctcttaatca gtggtggaag 180aacggtctca gaactgtttg tttcaattgg ccatttaagt ttagtagtaa aagactggtt 240aatgataaca atgcatcgta aaaccttcag aaggaaagga gaatgttttg tggaccactt 300tggttttctt ttttgcgtgt ggcaagtttt aaagttatta agtttttaaa atcaagtacc 360tnggn 365 242 625 DNA Homo sapiens misc_feature (1)...(625) n = A,T,C or G 242natngganng nttttccctt aacgtgggcc ncggccgagg nacttttttt tttttttttt 60tttttttttt gcaggcagct atttaattan gntcttaana catttanaac nccaatttgn 120gaanataaat tccattcgtc anaacaaacn cagatcgcan gtagccctgg anctgangaa 180taactttgat ttttggnaaa atttgngagt ccncagcttt ctgatcaatc ttgcgctgct 240cccnaatctc atatttctct ttttctgggg ccaaaatctt accttcctgg ngtctgggct 300ttcgcaactt cttcttcttg aaagaagcct cagtaaaaat ggtttgggaa ttttacatta 360ctgatatcca atttnggtga aatggcaatg accaatttct nggggggtct tcgtaaaaga 420actccantga nggnccaaag gtccagtccc aagtataggc nctnaccact gnttcaggaa 480accacctttt gncctggggg gtccatgagg atgaccaaat ggncccgggg naagctggct 540ccantttttt acggcctacc gaagggtttt tgccngggta aaagttttag ggccattttc 600ngggnaaatc taggcttttg gaaat 625 243 639 DNA Homo sapiens misc_feature (1)...(639) n = A,T,C or G 243nncnaattcc nccntaaccn ggnccccgnc caagnacccc ggcncctttg gatgtatnga 60aatnaacnta ttaatgggga cntattggag aaggaaatnc ctagacctac aactttnagc 120naatagcngt gatgttttag gaactgaaat gtcacactta aagtcttnag cccagctact 180tccctatttt tgtggggaga aaanggccng attagaactg ttctggttgt gtttggcggg 240aggggaataa tttttgttca gtccttctta gtgaccaaac tttaattttt aagaataata 300tattgactta ctgaactgaa gcattctgag ttgaaaggag ctccncagga ntggagttct 360gtgttgctca catgttnaaa ncttgctcac cttnatagcn caaggaatac ctatcttcca 420natnccgcca ttttcatctc ttaaatgnag tccaaagtat gacttgagaa agttgctctn 480ggattctggg gtcttaaaac tngggattct gggattntgg ggtccnaaag ttnaccttgn 540aaagttgcct gggnttttan aaatncnctg nattctgggg ttttaaaaaa ttttgaaaaa 600accccncccn ncttgaaagg gaccttaaaa attaacctn 639 244 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 244tcgagccgnc ggcccgggcc aggtactttt tttttttttt tttttttttt gaaaatggag 60tcttgctctg ntgccaaact ggantgcaat ggtgcganct gggctcactg naatctccac 120ctnccgggtt caagcgattc tcctgcctca cctccgagta actgggacta caggtgcgcg 180ccaccaagcc cagctcattt ttgnattttt agtanaaatg gggtttcacg atgttggcta 240ngatggnctc gatctctggt caaagtcttt tctgnaaata tccttggtaa aaaaacaatt 300ttagactgta gctgttgcaa atgctttaag gaagaaacna aacaactgca gtcttcctga 360aatgaaaaaa ctccccaggg ctgctattna aaacaacccc accagcactt caatcatgat 420gccnacagtg gcccactgaa aaancnggaa aagttcnaat cccaaactgg gatgctcttg 480actntggaat tntgngggcn ntncccnant ttnanacaaa acngnctngg nccctntttt 540ttgggggaat ttgggaanaa aaaaacttgn gngttcttgn ggttccnttg ttccccaaaa 600nactgggggn nggg 614 245 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 245gccgtggtcg cgggccgagg tccatttgcc tcccggcctc aagccgattc tcctgcctca 60gccctccaag tagctgggga ttacaggcac ctgccaccat gcccggctaa tttttgnaat 120tttagtagag acagggtttc accatgttgc ccaggctggt ttcgaactcc tgacctcagg 180tgatccaccc gcctcggcct ccaaagtgct gggattacag gcttgagccc ccgcgcccag 240ccatcaaaat gctttttatt tctgcatatg ttgaatactt tttacaattt aaaaaaatga 300tctgntttga aggcaaaatt gcaaatcttg aaattaagaa ggcaaaaatg taaaggagtc 360aaaactataa atcaagtatt tgggaaagtg aagactggaa gctaatttgc attaaattca 420caaactttta tactctttct ggatatacat tttttttctt taaaaaacaa ctttngatca 480gaatagcccc atttagaacc ttttggtatc agncaatatt tttaaatagt tnaaccnggc 540ctaagctnaa agnggcttga tntgagtaaa cttttcaact ggcttgaacc ctnaaccttt 600taaaatgacc ttccgagntt 620 246 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 246acttattctt caggggttac tgagtcggca cctatgacag ctaagagagc tttcttaaag 60actgcctcag tgtcttcttg gcttttggca ccttcactcc actctgccca ggaaatccac 120aatggcagac aaacctgggg tttcaggtgc acaaagactt cttcaaaaag catggctatg 180tcagggctct ttgactcgat cagcacctgc agcttcagct gccacattgt cccagagtct 240ctaaacaatt caagttccag ctactgncac ttccagagct tcctcaggaa gttataacac 300agcaacgaaa cactcaactg cttgtattgg cattctgaca gaagcttcaa gttcatgtgc 360cttcctgaat acagtcatgg tctttncaac ctcttcctct aaggacccac tatttgactt 420cttaataaat ctttccagcc aaaggngatg aacactttca catgggcctt gtggcaaaag 480cttnatggct ttttatcncg gacagacctt tctcttcggg cgacctcaat ggtttggctt 540ggtcgtggag ctggtntttg gctnggactc aacttnaatn ttgcttgccc naaac 595 247 364 DNA Homo sapiens 247gggtacacta gaaagtcttt tacaaaataa tcatcttaga tcaacagaag accaatcttc 60aatgtcgtcc tgcaagatgg gttactttaa catctcctcc tgttttctcc aatgttctcc 120tttagtatgg ctggtaattg ttttggtgat tgccaccccc tcgagatgcc ttgccataag 180tgctctgttg gccactgtag tctgcatatc cctgtccata tccatagttc ccatagttat 240acccagtata atcatatccg ccatagccac tatagttttg atcaccacca taggcactat 300tgtaatttcc atatccttga tcataatagt tattaaatcc ttggttccag ttttggccct 360gacc 364 248 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 248ggtncagata tcttcaaagg aggaagaaga aagggaaacc agatggtgga cctgaatatg 60ncccttancc aganctaatc aacccactca gccagaatag aagaagctgg aatagattcc 120ccaacctggt ttgccagttc atcttttgac tctattaaaa tcttcaatag ttggtattct 180gnaatttcac tctcatgant gcnactgngg cttaactaat attgcaatgn ggcttgaatg 240taagtagcat cctttgatgc ttctttgaaa cttgnatgaa tttgggtatg aacagattgc 300ctgctttccc ttaaataaca cttaaaatta tttggaccag tcagcacaac atgcctnggt 360tgnattaaag cnnggatatg ctggatttta taaaattggc caaattagag aaatntagtc 420ccatggaaat atatttcttg taaaaaagtg cttgaatctt tttggtcaag ataatgccac 480tcttaagaat atcttcncac tnttgangga ttaaatatcg gcantggaaa agccttaaaa 540atggggtcna cttgccttgn gcctaaaccg accctgaaat gggatttccc n 591 249 332 DNA Homo sapiens misc_feature (1)...(332) n = A,T,C or G 249actctccgag agggtcgttt tcccgtcccc gagagcaagt ttatttacca aatgttggag 60taataaagaa aggcagaaca aaatgagctg ggctttggaa gaatggaaag aaagggctgc 120ctcaagagct cttcagaaaa ttcaagaact tgaaaggaca gcttgacaaa ctgaagaagg 180aaaagcagca aaggcagttt cagctttgac agtctcgagg cttgcgcttg cagaaacnaa 240aacagaaagg ttgaaaatga aaaaacccag ggtaccttgg nccgggacca cgcttaaggc 300gaaattccaa cacacttggc cggccggtac ta 332 250 626 DNA Homo sapiens misc_feature (1)...(626) n = A,T,C or G 250ggtactatta gccatggtca accccaccgt gttcttcgac attgccgtcg accggcgaag 60ccctttggcc cgcgtcttcc tttgaactgg ttgcagacaa gggtcccaaa ganagcagaa 120aattttcgtg ctctgagcac tggagaaaaa ggatttggtt ataagggttc ctgctttcac 180agaattattc cagggtttat gtgtcaaggt ggtgacttca cacgccataa tggcactggt 240ggcaaagtcc atctatgggg aagaaatttg aagatgaaga acttcatcct aaagcatacg 300ggtcctggca tcttgtccat ggcaaatgct ggacccaaca caaatgggtc ccaatttttc 360atctgcactg gccaagactg antggttgga tggcaaanca tgtngtgntt ggccaaagtg 420aaagaaggca tgaatattgt ggaaggccat ggaacgcttt tgggtncnag gaatggcaag 480aaccnccagg aagaatcacc cnttnttgac tggggacaac tcnaataagt tgacttgggg 540nttaatntaa ccccccanca attccttttg gaactcagga aacacccttc ancccanttn 600tttcaanttc caaaannttg ggcctn 626 251 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,C or G 251actttttttt tttttttttt tttttttttc aacagaagaa cttttngttt ctttattttc 60aatattngtc ttattaatat ttttcttatt ttataatgca attacaacaa tttaggagac 120aaaacantat aaacaaaaga atgttaaata gtttttttta aaaaatagct tgttgcttgc 180aagaaagtcc atataatctt attccccccc aaatataatt ttatactttg cactaaacca 240aaatagctta tggaaaatta ggtattaaat agctaaacac agaaaaccta cagctataaa 300taacataaaa tacagtttaa ctttaatgng atgcttaaac aaagcaaact atgatgcant 360atgaatcaac ttcattaatt ggacaagtcc agtgaggcnc aaattagata agcnctaaac 420cctcatgatg ggcaagtgaa accttcaccc cagcaagggt ctttcnggtc ttggctatgc 480caattccttc canaaaagnc ccagttttac angtctggct ttttccgggg gaacccccca 540tttntttnnc ccaagttggt tnggatttgg cccccannaa attttttttg gngnaaaaan 600aan 603 252 500 DNA Homo sapiens misc_feature (1)...(500) n = A,T,C or G 252actttatttg ttttttttgt tttgttttgg tttttttttt ggcttgactc aggatttaaa 60aactggaacg gtgaaggtga cagcagtcgg ttggagcgag catcccccaa agttcacaat 120gtggccgagg actttgattg cacattgttg tttttttaat agtcattcca aatatgagat 180gcattgttac aggaagtccc ttgccatcct aaaagccacc ccacttctct ctaaggagaa 240tggcccagtc ctctcccaag tccacacagg ggaggtgata gcattgcttt cgtgtaaatt 300atgtaatgca aaattttttt aatcttcgcc ttaatacttt tttattttgt tttattttga 360atgatgagcc ttcgtgcccc cccttccccc ttttttgtcc cccaacttga gatgtatgaa 420ngcttttggt ctccctggga agtgggtgga ngcagccagg gcttacctgt accttggccg 480cgaacaccta aggccaantt 500 253 634 DNA Homo sapiens misc_feature (1)...(634) n = A,T,C or G 253tcgagcggcc ngcccgggca ggtactatta gccatggtca aaccccaccc gtgttcttcg 60acattgcccg tcgacggcga acccttgggc ccgcgtctcc tttgagctgt ttgcagacaa 120ggtcccaaag acagcagaaa attttcgtgc tctgagcact ggagagaaag gatttggtta 180taagggttcc tgctttcaca gaattattcc agggtttatg tgtcaggggt ggtgacttca 240cacgccataa tggcactggt ggcaagtcca tctatgggga gaaatttgaa gatgagaact 300tcatcctaaa gcatacgggt cctggcatct tgtccatggc aaatgctgga cccaacacaa 360atggttccca gtttttcatc tgcactgcca agactgantg gttggatggc aaacatgtgg 420tgtttggcaa antgaaagaa ngcatgaata ttgtggaagc catgganccc tttnggtcca 480ggaatggcag aacnnccagg aanacaccct tgntgactgt ggcaactcga ataaattgac 540ttggggttat cttaaccncc caacattcct ttggacttag gaancanccc ttcancccnt 600tggttcaant tcccaaaaat ttgggctncc tnng 634 254 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,C or G 254nctttttttt tttttttttt ttttttaaat taattaatta aaaaataggt ggnctactgg 60tggtccttaa gctggaantg cagtgggcac aatcatggnt cactgnagtc tnaacctncc 120aggttcaagt gatcctccta cctcacctcc antagctggg attacaggca tatgcgacca 180tgcccagcta attttttatt ttttgtaaaa acggggtctc actatgtcgc ccangctggn 240cttgaactcc tgaactcaag tgacccttcc gnctnacctn caaagtgcta ggcttacagg 300tgtgaaccac catgcctggc ctaaaaaatt tattttaaaa aagtaattta tctcttacag 360ttgtggaggc tgagaaatcc aangncaant ggcncatttg gtgaaaacct tnttgctggt 420ggggactctg tgaaatnccc aantggcnca tgcatnacac antgangggg cttacattcc 480aacatgctat ctcttttaag ttttaaagta cnggccnaaa tntgaacntg aatgacttna 540aatccacnca ttccnctttt ggacnaaaaa ccntgggcaa ttgggatctt ggcnttttna 600aa 602 255 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 255cgaggtacag gtaagccctg gctgcctcca cccactccca gggagaccaa aagccttcat 60acatctcaag ttgggggaca aaaaaggggg aagggggggc acgaaggctc atcattcaaa 120ataaaacaaa ataaaaaagt attaaggcga agattaaaaa aattttgcat tacataattt 180acacgaaagc aatgctatca cctcccctgt gtggacttgg gagaggactg gaccattctc 240cttagagaga agtggggtgg cttttaggat ggcaagggac ttcctgtaac aatgcatctc 300atatttggaa tgactattaa aaaaacaaca atgtgcaatc aaagtcctcg gccacattgt 360gaactttggg ggatgctcgc tccaacccga ctgctgtcac cttcaccggt ccagttttta 420aatcctgagt caagccaaaa aaaaaaaacc anaccaaacn nanaaaccaa ttaagccatg 480ccaatctcat ctggtttctg cncaagtang gttgncaaaa aagggttacc ncactaantc 540ntagccccta aaccnttgcg ggggncantg angggccgan tttganactc cggntggtga 600nccanttggn ggag 614 256 308 DNA Homo sapiens misc_feature (1)...(308) n = A,T,C or G 256ncntccagca gtgggtcatt cgncaacgaa agtcntaccg tagaaaagat ggcgtgtttc 60tttattttga agataatgca ggagtcatag tgaacaataa aggcgagatg aaagggtctg 120ccattacagg accagtagca agggaatgtg cagacttgtg gccccggatt gcatccaatg 180ctggcagcat tgcatgattc tccagtatat ttgtaaaaaa taaaaaaaaa ctaaacccaa 240aaaaaaaaat nnnannnaac annnnanaaa aannnnaaaa aaaaaaagta cctnggccgn 300gaccacgc 308 257 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,C or G 257gcgtggtcgc nggccgaggt acgcggggga gacaaaccat accatatccc accagagagt 60cgcagacact atgctgcctc catggccctg cccagtgtat cttggatgct gctttcctgc 120ctcatgctgc tgtctcaggt tcaaggtgaa gaaccccaga gggaactgcc ctctgcacgg 180atccgctgtc ccaaaggctc caaggcctat ggctcccact gctatgcctt gtttttgtca 240ccaaaatcct ggacagatgc agatctggcc tgccagaagc ggccctctgg aaacctggtg 300tctgtgctca ntggggctga gggatccttc gtgtcctccc tggtgaagag cattggtaac 360agctactcat acgtctggat tgggctccat gaccccacac agggcaccga acccaatgga 420aaangntggg antggaataa cantgatgtg atgaattact ttgcatggga gagaaatcct 480tcancatttt naaccccggc cctgtccaac ctntcaaaaa cncacatttt taaggggaaa 540attttactgg atggganggt acccttttnt ggaagtactg cttttcngga nggaagtacc 600cc 602 258 600 DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 258ggtgtntgng ncttatntgt agcggcgcgg ntggttctga aatcgccttc agcggcgccg 60cagtcntatt atgtgnatgt ccctaccacn aaaatncaga ttaattggna tgctcattac 120ccacgtgaac gccaaagccc ttcgaagtag tgctgccctg cactnaatca agaagttgca 180ttaaaattag aaccaaatcc agagtcactg gaactttctt ttaccatgcc ccanattcag 240gatcagacac ctagtccttc cgatggaaag cactagacaa agttcacctg agcctaatag 300tcccagtgaa tattggtttt atggggatag gtgatatggn caatgaattc aagttggaat 360tggnagaaaa actttttgct naagacncng aagcnaagaa cccattttct actnaaggca 420cagatttaga cttggagatg gtagcttcct atatccaatg gatgatgctt tcagtccgtn 480cnttgatcag tgncacnttn gaaagcagtt cccaagncct gnaacccagt cctaagccaa 540gtccggttcn gcgattaatc cgactatgta tgcccttcat ngcccctgtn ataaacnggn 600 259 600 DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 259gccgaggtac atgggaaagg gagtatggng agctatttcc tttttaaagg atgaagacct 60tcataaattg gcccctcgga ttctggtgat tcccgcccgc aagcgcaaat gctccagtgn 120gttatgaaaa tgnttgntaa tctgctctgg ttcttcactg gattcaagan tcgggaggnc 180ttctcgaatc ttttggataa nctggtttaa aacctgaatt gntacccgca tcattttcct 240tttcataaaa atagatatat ctgntcagaa tttctatnaa aagctgcact tgtaganang 300ggtccatgca ctgatttgct atttttaaag ctttttttan gcactccatt accctnttgc 360cttcgtgaaa cttcttccca tttttgnccn ggttctggcn gaccngaaga aatgtgccca 420agtgcttaca agttnggcct gacaaggttc nttaaaantt tggatgtacc aagggccccc 480tgggtcctca aaggtcatga atctttttac tggaaccctt atcctttnaa aaggccatgg 540tcaagggaat gnncttcttg gctttgaaac ccggattaan tttttncaaa aaaagccngn 600 260 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 260acgcgggaac tccatcctca ccacccacac caccctggag cactctgatt gtgccttcat 60ggtagacaat gaggccatct atgacatctg tcgtagaaac ctcgatatcg agcgcccaac 120ctacactaac cttaaccgcc ttattagcca gattgtgtcc tccatcactg cttccctgag 180atttgatgga gccctgaatg ttgacctgac agaattccag accaacctgg tgccctaccc 240ccgcatccac ttcctctggc cacatatgcc cctgtcatct ctgctgagaa agcctaccat 300gaacagctta ctgtagcaga gatcaccaat gcttgctttg agccagccaa ccagatggtg 360aaatgtgacc ctcgccatgg taaatacatg gcttgctgcc tggtataccg tggtgacntg 420ggtnccaaag atgtcaatgc tgccttggca ccattcaaac caagcgcaga ttcaatttgg 480ggatggtgcc cactggcttt aaggtngnat naactaccag cttccactgn ggnnctggtg 540gaaactngcc aaggnncctt ggccggaaca ccctangggg aattcanncc act 593 261 343 DNA Homo sapiens misc_feature (1)...(343) n = A,T,C or G 261cctacctctc ttnccactgc aaatttctgg gatagaccaa aagtgaattt gattatgtgt 60tggctgaagt tcttcattct gactgttgan gggaggtttt cctttgaaga gttttcatcc 120cagactcagc tgtcttttca catggatgaa ataattcctg ctaccaacaa cagagcttca 180ccaggaagtt gagttttcaa gatgccttgt tgctttgaag aagggagtga tgtcaattct 240cttgntacat tctcccttta gcaacctgag taagagactc tctgccactg ggctgcaaaa 300aaataaatta cttgaatctc cccttggccc angctgaggt acc 343 262 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 262actttttttt tttttttttt tttttttgtt tttttttttt tttttttttt tttttttttt 60tttttttttt ttacagngtn ttttcatttt tattactcaa aaaagtttca tttttttnat 120ttanctttnt gactntgggc ttgggccttn aacantttca naacgatttt ntgctcctcg 180anaaggaaag cncccttgat cctgncacna acncntttag cncacatgga accnccatag 240gccctgntga catgtttctt tgtttnggac aatntcataa aaactttagg nnttacagca 300cnaacccctn naagtntgcc tgggcncaca ccanatgcaa attttggggc tttcccaacc 360ttnttggnat aaaggtaaac aattttatta ccagggggtt cgggacaacc tanttttgtt 420aaaggctgta ttgtaggaaa acctacctcg ggatgtcaaa cccttnacca ttttgagggn 480ctggaaanaa ngttcccgga aanccccggg tancttnggc cggaaccccc taangggnga 540attccnaccn cttgggggcn gtantaaggg ganccaantt gggccaaant tgg 593 263 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 263accaagagtt tgctcctggc tgctttgatg tcagtgctgc tactccacct ctgcggcgaa 60tcagaagtaa gcaactttga ctgccgtctt ggatacacag accgtattct tcatcctaaa 120tttattgtgg gcttcacacg gcagctggcc aatgaaggct gtgacatcaa tgctatcatc 180tttcacaaag aaaaagttgt ctgtgtgcgc aaatccaaaa cagacttggg tgaaatatat 240tgtgcgtctc ctcagtaaaa aagtcaagaa catgtaaaaa ctgtggcttt tctggaatgg 300aattggacat agcccaagaa cagaaagaac cttgctgggg ttggaggttt cacttgcaca 360tcatggaggg tttaatgctt atctaatttg tgcctcactg gacttgncaa ttaatgaagt 420gatcatattg catcataagt ttgctttggt taancttaca ttaaagttaa ctggatttta 480agggaattat actgtaggtt ctggggtaac tatttaatac taattttcat aacnattttg 540gttaatncca agttnaaatt tatttggggg gaanaaaatt tttggccttc t 591 264 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 264accaagagtt tgctcctggc tgctttgatg tcagtgctgc tactccacct ctgcggcgaa 60tcagaagtaa gcaactttga ctgccgtctt ggatacacag accgtattct tcatcctaaa 120tttattgtgg gcttcacacg gcagctggcc aatgaaggct gtgacatcaa tgctatcatc 180tttcacaaag aaaaagttgt ctgtgtgcgc aaatccaaaa cagacttggg tgaaatatat 240tgtgcgtctc ctcagtaaaa aagtcaagaa catgtaaaaa ctgtggcttt tctggaatgg 300aattggacat agcccaagaa cagaaagaac cttgctgggg ttggaggttt cacttgcaca 360tcatggaggg gtttagtgct tatctaattt gtgcctcact ggacttgtcc aattaatgaa 420gttgattcat attgcatcat agtttgcttt ggttaagcat cacattaaag ttaaactgga 480ttttatggta tttatagctg nanggtttct ggggttanct atttaatact aaatttccat 540aagctttttg ggttaangcc aagnttaaaa tttttttggg ggggaaaaaa atttt 595 265 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 265ggtacttttt tttttttttt tttttttttt ttgaaaatta tacttttatt tgagtcacca 60ggagaaagat tcacttgtgg ttcaagtcaa atgttcanaa tcataacagg ccanaaaggt 120ttgatcccga gcacaagccc acgagggagg ggaccaaaac agaccaaaat gagacaacaa 180ccccatataa aaagatgaac tggcggcttc acacactcac acacatacac atacacacgg 240atgaaatgtt tggacagagg caaatttcac gtggtcattt ctgtttcttt ttaaatacag 300gtttgtgggg tggtattttg ttttttccag ctataaaaaa aggcccaaaa gtgcatgtgt 360gaggggggaa aggcagaaat taagcaataa agtcattttc cctggaggga catganaggg 420agaaaacagg aggcaattgc tggganaacg cactttctta acactgggct tttgggtatt 480cttantattg gnccncaaaa agttattttc acattctaac tttgaagnct ntttccnggg 540attnaatggn ccttaaaacc tttgggaact ttaaaaaaac cngggcttac cc 592 266 594 DNA Homo sapiens misc_feature (1)...(594) n = A,T,C or G 266acgcggggaa aaaaaaggca gtattccctt tttaaatgag ctttcaggaa gttgctgaga 60aatggggtgg aatagggaac tgtaatggcc actgaagcac gtgagagacc ctcgcaaaat 120gatgtgaaag gaccagtttc ttgaagtcca gtgtttccac ggctggatac ctgtgtgtct 180ccataaaagt cctgtcacca aggacgttaa aggcatttta ttccagcgtc ttctagagag 240cttagtgtat acagatgagg gtgtcccgct gctgctttcc ttcggaatcc agtgcttcca 300cagagattag cctgtagctt atatttgaca ttcttcactg tctgttgttt acctaccgta 360gctttttacc gttcacttcc ccttccaact atgtcccaga tgtgcaggct cctcctctct 420ggactttctn caaaggcact tgacccttcg gnctctactt ggcccctnac ctcaccccct 480tctggcaccg gncntgngac attcacttcn gagaagaccn cccccaagga ggcnggcgnt 540tggnccanga aaaaaccccg gggaagggtt tntttttttn aaagggaaat ttcc 594 267 598 DNA Homo sapiens misc_feature (1)...(598) n = A,T,C or G 267actggccctc ggtgctggca aaggtgtagt tccactggcc gagggaatca agacatagtg 60gtccttctgc taagccaagg gctgccacaa tgacacagta gccagatcct gcaattccaa 120tgagagcagc caatacagaa gaaagcatcg cacatcgttt gccacagttt tcatggccac 180agcagccaca gcagtcatcc tgttccagcc caatgaagac aaatgctggc aggagcatca 240gcagggccac ctcctacgat gccagaaaag aaccacacga aacggctgag gtggttttcg 300gaggcatact ttgttcccat tgggaaagta aagccaaata ttacccgcga tgcacaggaa 360ggggcgagcc caaccagaaa atgtccgaat gcatcgtgca cacttcccat agcacatggt 420ggtcttgcta ggtttttctc ccccttctct ttggncttca acttcagtga taccccaaat 480tagatgaaag tggtgccctt ttgggtggaa aaagcaaaca ccaaccccgg gtacctttgg 540gccggaacac ncttaaggcc aattccannc aattggcggc ccgtacttan gggatccc 598 268 590 DNA Homo sapiens misc_feature (1)...(590) n = A,T,C or G 268ggacatatta tcaataggct ataagatgta acaacgaaat gatgacatct ggagaagaaa 60catcttttcc ttataaaaat gtgttttcaa gctgttgttt taagaagcaa aagatagttc 120tgcaaattca aagatacagt atcccttcaa aacaaatagg agttcaggga agagaaacat 180ccttcaaagg acagtgttgt tttgaccggg agatctagag agtgctcaga attagggcct 240ggcatttgga atcacaggat ttatcatcac agaaacaact gttttaagat tagttccatc 300actctcatcc tgtattttta taagaaacac aagagtgcat accagaattg aatataccat 360atgggattgg agaaagacaa atgtggaaga aatcatagag ctggagacta cttttgtgct 420ttacaaaact gtgaaggatt gtggtcacct ggaacaggtc tncaatctat gtagcactat 480gtggctcanc cttggtaccc cttggattat atatcaacct gnaacatgng nctgggactt 540actttcnaaa cnaaatnttc cttntttgaa gaaaatctgg gtttttgnaa 590 269 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,C or G 269acttgaagga agtcgaatca gagatagact ctgaagaaga acttataaat aaaaaaagaa 60tcatagagaa agttattcat cgactcacac actatgatca tgttctaatt gagctcaccc 120aggctggatt gaaaggctcc acagagggaa gtgagagcta tgaagaagat ccctacttgg 180tagttaaccc taactacttg ctcgaagatt gagatagtaa aagtaactga ccagagctga 240ggaactgtgg cacagcacct cgtggcctgg agcctggctg gagctctgct agggacagaa 300gtgtttctgg aagtgatgct tcaggatttg ttttcagaaa caagaattga gttgatggtc 360ctatgtgtca cattcatcac aggtttcata ccaacacagg cttcagcact tncntttggt 420ggtggttcct ggtcccntgg aagttggaac caaattaatg gngtagtctc tatacccaat 480acctttggtt ttcatgtgta anaaaaaggn ccattacttt taanggattg tgctggnctt 540attgngccan taactttttt ttaaatggcc cagttacngg ttttaattct taaaannaaa 600aa 602 270 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 270ggtacgcggg ggtaggagcc tctctcccta ctgctgctac acaagaccct gagactgacc 60tgcaggacga aaccatgaag agcctgatcc ttcttgccat cctggccgcc ttagcggtag 120taactttgtg ttatgaatca catgaaagca tggaatctta tgaacttaat cccttcatta 180acaggagaaa tgcaaatacc ttcatatccc ctcagcagag atggagagct aaagtccaag 240agaggatccg agaacgctct aagcctgtcc acgagctcaa tagggaagcc tgtgatgact 300acagactttg cgaacgctac gccatggttt atggatacaa tgctgcctat aatcgctact 360tcaggaagcg ccgagggacc aaatgagact gagggaagaa aaaaaatctc tttntttctg 420gaggctggca cctgattttg tatccccctg tagcagcatt actgaaatac ataggcttat 480atacaatgct tctttctgga tattctcttg gcttgggtgg accccttttt ccggccccag 540aattgttaan taatngaann nccntncann aagggnnnaa aggnaaatca ncttt 595 271 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 271ggtacattga gatcccgcct ctacaaaatc aaaaaattag ccaggcaagg tggtgcgtgc 60ctgtcgcccc agctacttgg caggctgagc tcaggaggtc aagcctgcct tgggccatga 120tcatcccatg cactccagcc tgacattcag agcaagacct tgtctcaaag aaagaaaaac 180atttttatgg tgttttcttt tttagtcttt tcaataatga aaattttcat tttacaggta 240aaatgaaagg cctggcattt attcaagatc ctgatggcta ctggattgaa attttgaatc 300ctaacaaaat ggcaacctta atgtagtgct gtgagaattc tcctttgaga tttcagaaga 360aaggaaacaa tgtgattcaa gatatttaca taccagaagc atctaggact gatggatcac 420tgtcccgatt caaattattc ttcagtccat ttcccctttc tatttcagct ggtccttttc 480acctaactgt cagtcattct ggtttcaacn atgctttatc tcatgtcctt gaatatagtt 540ggggnacttt aatttttang gaataatnna acagnttccn ttaaaggntn ng 592 272 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 272acattaaagt gtgatacttg gttttgaaaa cattcaaaca gtctctgtgg aaatctgaga 60gaaattggcg gagagctgcc gtggtgcatt cctcctgtag tgcttcaagc taatgcttca 120tcctctctaa taacttttga tagacagggg ctagtcgcac agacctctgg gaagccctgg 180aaaacgctga tgcttgtttg aagatctcaa gcgcagagtc tgcaagttca tcccctcttt 240cctgaggtct gttggctgga ggctgcagaa cattggtgat gacatggacc acgccatttg 300tggccatgat gtcaggctcg gcaacaggct ccttggtgac actcaccaca ttgnttttca 360agctgacttt cagcttgnca ccttggagag actttaaccc ggaccaaggg cccgatgcct 420tccgttaccc aggaatttca tcaccaatgg tggtanttca ggaatgttgg caagtttcct 480tggcatnttc ccaaanagtt tgttcccgtt cttnttgggn ggcangggct tcggaaaggg 540ttnattttgt ngggaaccna aaaactgggg tnaaactcct tnccggttna ngggtttccg 600nnanccn 607 273 398 DNA Homo sapiens misc_feature (1)...(398) n = A,T,C or G 273ggtaccgcca ttattctttt gggcaccttt ggttgttttg ctacctgccg agcttctgca 60tggatgctaa aactgtatgc aatgtttctg actctcgttt ttttggtcga actggtcgct 120gccatcgtag gatttgtttt cagacatgag attaagaaca gctttaagaa taattatgag 180aaggctttga agcagtataa ctctacagga gattatagaa gccatgcagt agacaagatc 240caaaatacgt tgcattgttg tggtgtcacc gattatagag attggacaga tactaattat 300tactcagaaa aaggatttcc taagagttgc tgtaaacttg aagattgtac ctgccccggg 360ccgnccgctc gaaagcttaa ntggccgttt cnaanncg 398 274 587 DNA Homo sapiens misc_feature (1)...(587) n = A,T,C or G 274actttttttt tttttttttt tttgttgaat caaaagcagg gtttattttt ctatcaaatc 60cccaatccat gttccagcca atggatgaag ggtgaatcaa gccccacata gactcttggt 120aaaaacaatt ctaactttct aaaaaaaaaa aaagccaaca cacttttttc tttcttttca 180aaaagctccc aggcctttgg gaacagctga aacaaattca tatcctgact aggtctgttt 240tctcttaggt atttggatgg tccctctctg ctgccacttc tgcacagatg aggcactgat 300aatggcctgc aggtcactca caatcctagc tccacatcac tccatggttt gataacctag 360aaccacgtta tgatttccat ttataatgcc ctaagaacag ctgaaaagat ctgtattaaa 420ttctgcaaat ctttattgag tgccactatt tgctgggcac angctaggcn ctggattctg 480ctggttcttg agaaacctaa aanggnncct tnggccggaa cacccttang gcgaaatcca 540cncactgggg ggcgtactaa ngggatccaa ctttggncca acttggg 587 275 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 275actttttttt tttttttttt tttgccttta taagagaatt tttattgtta attatttacc 60ttaatagttt cagaaagagg aacaaattag ctcagtccaa catgattggc agttggcata 120ttctagtgaa gcaagtgttc tgactgctaa ggatttaatt tggataattt taatacttag 180ccatctaaca cttcaagcat aacccagaat aaatgcacca ccttcctttc actttaatac 240ccgnacctac ctcacttcga tataagaaat atcattcaat atgatttcca gaagggacaa 300gtttcctgga gaatacaggc atganggaca atgcacaaaa agaaaaactc aaaatnaaac 360tctggatgga taattactaa gctaagggaa ccaaaccttc caatttntaa agaaattaaa 420tccggttcca aatgcctnat angnctatgt tnaaaaggtt ctggattaat accggaaaag 480gnttgnttnt tacaggatnc cccaaccgtt acgggccctt ngcccagaat gggccttaaa 540anccaaagng tcttttccgn ngaggcccca tttnanaatc cttntttt 588 276 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 276actttagata catcattcct caaaaagttt ttaacggaga aagtggggca attcaatggg 60ggaaaggacg gcctttttaa caaatggtgc tggttctact gggtatctgc atccttgata 120cacagaagtt aactcaagat ggaccacaga ctcacatgta agagctaaaa taacattctt 180agaagaaatc atggaagtaa atcttcgtga ccttggatca ggtaatgggt actttttttt 240tttttttttt ttttttttta tcagattaat tttactttat ttcttcaggc ctggggtttt 300tcgatgactt caaatttggg atcttcaaat ttgaaggtgg gaaatggtat tcatgtctgc 360attaccaaac atttgctttg acttaaaaag ctcctctcca gctcttgccg atctctgaac 420tagcatcaac aggntcctcc agatgtctgg nccttaaatt tggattccct aatcttggcc 480acaaagangt ttcttggata gggaacaaag ttcccttatt naaatgccan tngtngaacc 540nccaatgttc cttcncaaaa ngggcttaaa ccggttaccc aattgacaaa ggaaa 595 277 597 DNA Homo sapiens misc_feature (1)...(597) n = A,T,C or G 277ggtactgttc ctgttggccg agtggagact ggtgttctca aacccggtat ggtggtcacc 60tttgctccag tcaacgttac aacggaagta aaatctgtcg aaatgcacca tgaagctttg 120agtgaagctc ttcctgggga caatgtgggc ttcaatgtca agaatgtgtc tgtcaaggat 180gttcgtcgtg gcaacgttgc tggtgacagc aaaaatgacc caccaatgga agcagctggc 240ttcactgctc aggtgattat cctgaaccat ccaggccaaa taagcgccgg ctatgcccct 300gtattggatt gccacacggc tcacattgca tgcaagtttg ctgagctgaa ggaaaagatt 360gatcgccgtt ctggtaaaaa gcttggaaga tggccctaaa ttcttgaagt ctggtgatgc 420tgccattggt tgatatggtt cctggcaagc ccatgtgtgt tgaaagcttc ttaaactatc 480cacctttggg tcgctttgct ggtccngatt tgagacanac catttccgnn gggtggcaat 540caaaccattg ggccaanaaa gnttntggac ttgcaagggn nccaaatttt ncccaaa 597 278 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 278ggtacttttt tttttttttt tttttttttt ttagtttatt aaaatactga gttttatttc 60acatgtatat ttttgtctcc ccaccatttc catgtctgac caccgctact actatgtcct 120atcataacat tccatacata cttaaaacca agcaaagggt ggagttccat ctttaaaaac 180taaacaggca ttttggacaa cacattcttg gcaatagaac ctggacaaca tttatcaaac 240acggtaggga aagttctcac tctgcattat aaaaaggaca gccagatatc aactgttaca 300gaaatgaaat aagacggaaa attttttaac aaattgntta aactattttc ttaaagagac 360ttcctccact gccagagatc ttgaatagcc tcttggncag tcattccgga aacaattctt 420ccataattga tgaatttggc tttcactttt gggaagagaa cccccttttc tatacttggg 480tgcattttgc ttaaaggctt ctacaaacta gggcctttgg gggtttaaga gttttccngg 540gtcttgaagg ntcttggcct ttgaacttgg ggtnaaaang gttgngcttt tccat 595 279 586 DNA Homo sapiens misc_feature (1)...(586) n = A,T,C or G 279ggtacgcggg gagatacgtt cgtcagcttg ctcctttctg cccgtggacg ccgccgaaga 60agcatcgtta aagtctctct tcaccctgcc gtcatgtcta agtcagagtc tcctaaagag 120cccgaacagc tgaggaagcc cttcattgga gggttgagct ttgaaacaac tgatgagagc 180ctgaggagcc attttgagca atggggaacg ctcacggact gtgtggtaat gagagatcca 240aacaccaagc gctccagggg ctttgggttt gtcacatatg ccactgtgga ggaggtggat 300gcggctatga atgcaaggcc acacaaggtg gatggaagaa ttgtggaacc aaagagagct 360gtctccagag aagattctca aagaccaggt gcccacttaa ctgtgaaaaa agatatttgg 420tggtggcatt naagaagacc ttgaagaaca tcacctaaga gattattttg acagtatgga 480aaattgaatg attgaaatca tgacttgacc aagcatggcc aaaaaagggc tttgctttga 540accttgagac atgattcngg ataaaatgcn tcnaatncnt ntggga 586 280 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 280actttttttt tttttttttt ttttttcttt tttttttttt tttttttttt ttttgaaaaa 60gtcatgaagg ccatggggtt ggcttgaaac cagctttggg aggttcgatt ccttcctttt 120ttgtctaaat tttatgtata cgggttcttc aaatgtgtgg tagggtgggg ggcatccata 180tagccactcc aggtttatgg agggttcttc tactattagg acttttcgct tnaaaacgaa 240ggcttntcaa atcatgaaaa ttattaatat tactgctgtt anaaaaatga atgagcctac 300anatgatagg atgtttcatg gggngtatgc atcggggtaa tccnaataac gtcggggcat 360tccggatagg cccaaaaang tttntgggaa aaaaagtttn atttaccccc attaaattta 420tnnnnaaaag ggattttgcc taaggttggg ctaagggggt ancccngaaa attgggggaa 480atcangnaat gaaaccccct ntgatggnca aaaacagctc ctnttggttg ggccttatng 540ggaanngggc ttcaacntan naccttnggc ggnaaaaccc ttanggngaa ttnnnnncaa 600ntgggggggg tn 612 281 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 281acgctgcttc ttcagagcaa tacgccgccg tttgtgctgc aggacacgtg gagtaacaag 60acgctgaatc ttgggtgctt tggtcctagg tttcttacct tctttattta agggctttct 120tacaacatac tggcggacat catcttcttt agagagattg aaaagtttgc ggattctgct 180agctcttttg gggcccaggc ggcgaggcac tgtagtatca gtcagtccag gaataccctt 240ctctcctttt tttacaataa ccaagttgag aacgctcaga tttgcatcca caatgcaacc 300acgaactgat tttctctttc tttctcagtt ctccttggtc tgtaacagga atgcccctta 360ctcaatanca ggcggacacg ggcatgggtc aagacaccct gcttcatggg gaaaccttgg 420ttgncgttcc accactggat tcggaccaca taaacctttc attcttnaac caaacgtaac 480ancaactttt ggnggccata cncttttata naaagtccgg ggganaagtn ttttgcagga 540caagcctgta acnaatagtn aaatcccgga tttggattcc taancctttt ccn 593 282 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 282ggtacaattc aagaaactaa gtatttatgg gcattgaaga aaaaatgttg agataaaatt 60gctgtgcaga aaaaagtgtt aatgaagccg acctgactac ttaaccttag agacctgctt 120tacaaggttg gcccttgatt ggcatctggg aacttggagt tcagggggct tccaccattc 180ccagaactga tcaaagtagc ttactatatc taaactgtaa aacaatatag tttctcctga 240acacctgctt tccttctggg agtctggaat tttggtatgt gccaggcaga gactaccttt 300gtgaccagct cccagtaaaa accccaggca ctcagtctct aacaagcttt tctggttgac 360agtgtttcac aagtgctggt acaactggtt gctgggagaa ttaagctcat cctctgtgat 420tccactggcc gaggattctt ggaagcttgc acttaagttt cccctgactt caccccatgg 480gcttttttcc ttgctgattt ggtttgnatc cttcctgnat aaatcatggc ctgaaccnaa 540cttgaaaaaa aaannnnnnn nnaaaaaaag gtncttgccc ggcggccgtt naaat 595 283 348 DNA Homo sapiens misc_feature (1)...(348) n = A,T,C or G 283actttttttt tttttttttt tttttttttt ctattttttt ttttttttgg ctntanaggg 60ggtanagggg gtgctatagg gtaaatacgg gccctatttc aaagattttt aggggaatta 120attntaggac gatgggcatg aaactgtggt ttgctccaca natttcanag cattgaccgt 180agtatacccc cggtcgtgta gcggtgaaag tggtttggtt taaacgtccg ggaattgcat 240ctgtttttaa gcctaatgtg gggacagctc atgagtgcaa nacgtnttgt gatgtaatta 300ttatacgaat gggggcttna atcgggagta cctnggccgn naccacnc 348 284 563 DNA Homo sapiens misc_feature (1)...(563) n = A,T,C or G 284ggtacccatt aatttgctca gatatagcag gcttaatggt tctatatttt caaaagtttt 60taagaatggt ttctaacgta ggagagggaa aacatccacc atcccttttc agaatttaaa 120tggagggcag taaacattct ttacacccaa aacctatggc agcagttcaa atttgaccaa 180ggtaaatgta gaatagagat gttctaaaca cagctaggac tcagcaagtc taacacacta 240aaatcatatg attacatttt aaaagaaaat gcacaaaaac caaatagaaa ttttgagatt 300ttttttcatt tgaaggtaat cttaatgcta ttaaattcac aaatgctaat ttaaataccc 360aatcctattt atctaaaaca cacattgcaa acacacaaat tatctattct ctccacatgt 420cagccgccca ttcatatcat ggtttggaaa tgggggagaa atagattncc cttaaactgc 480aagtcaacan ggggttcttt acagttaact ttagccaaat tcataccaaa tacccgggta 540cctgcccngg cggccgttcn aaa 563 285 422 DNA Homo sapiens 285acaatggact ggatactaga aattttcttt tcactcaaca gaacataggc atcctggaat 60tcacatttct gaccttttga tgtattaata aagtatggag aaatatagcc tcgatcaaac 120ttcatgcctt caataatttc taattcatca ttcagtgttt ttccatcctt tactgtgatg 180acaccctttc ttccaacttt tttcattgca tcagagatga tattgccaat ttctttgtct 240ccgtttgcag aaatcgtagc aacctgtgca atttcttcag gggtggtcac aggtttagac 300tgctttttaa gttcagcaat tacagcatca acagctaaca tcacacctct cctgatttcc 360actggattag cacctttgct aatcttctcg aagccttctt ggctatagag cgtgccagta 420cc 422 286 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 286actgttcctg caggttaagg caggactgga actcctccac agcttgcaca tagttttcag 60attcaacact aacttctccg agtttaagat gtgcctgggc agcataaagc tgtgcttctt 120ttgtttcttg ccttttaaaa atgatctttg ctaaatccag catatcccag gcaagctcta 180ggttcccaat ctcctcctcc tcattttctt gaagagactt gttttcaagg actgaatcat 240ttggcatttc ttcggtctta tcattttctt tatcatcctc ttctgagcct tcagtttcat 300ctatgttatc attattttct accagagatt catcttctgn tnttttctcc ttcttcctct 360tncacatgca caccttccaa ggcgtttcca acacaccatt cttcatcttg ccaacttcag 420aagtggattt ccatagaaaa agaangnttn ttcacactta ttaactgctc ttcatacttt 480ttacctnaaa gactaactgn ttcctggaat gcattggccg ctgctnggaa atccccatan 540cngaagttnt ggcctaancc aaagttntta gttactttcc catccgac 588 287 583 DNA Homo sapiens misc_feature (1)...(583) n = A,T,C or G 287actggaactc caggaagcgc tggccagcct catacgggag ccatttttct ttcactgcct 60ctgctgctga catcttcttc tttcccttca caccctcgaa gcctatgaag gctttctgag 120caggcttcag cctggtggcc atgtcttggt caatcacacc ctgggagact gcgtcctgaa 180gtgacagctt ctggcccgtg gttgggtgga tgatgccacc tgtgcaggcc tgagcctcca 240gaagcctctg acccgtgatg ctgtcaacga tgccccgctc tataccttct gtaatggaga 300ttttctccag gttttctgtg tcaaagatgg ctgcaatggg gctcgattct tncagggtgt 360ctgaaaaaga actgctcctt atggntaaat tcctgacctg gatatggtgg aaatcttact 420tactgattca tgtcgggagc tgctaaaaac atnatcgttg caccactggc catgctgtgn 480ttggngccac accatttttn angngacatg taacnaattg antaggttag nttccgaacg 540gaccttggcc ggaacaccta aggngatcan ncatggggcg tnn 583 288 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 288ggtacttttt tttttttttt tttttttgtt atttagtttt tatttcataa tcataaactt 60aactctgcaa tccagctagg catgggaggg aacaaggaaa acatggaacc caaagggaac 120tgcagcgaga gcacaaagat tctaggatac tgcgagcaaa tggggtggag gggtgctctc 180ctgagctaca gaaggaatga tctggtggtt aagataaaac acaagtcaaa cttattcgag 240ttgtccacag tcagcaatgg tgatcttctt gctggtcttg ccattcctgg acccaaagcg 300ctccatggcc ttcacaatat tcatgccttc tttcactttg ccaaacacca catgcttgcc 360atccaaccac tcagtcttgg cagtgcanat gaaaaactgg gaaccatttg gggttgggtc 420cagcattttg catggaccan aatgccagga cccctatgct ttaaggatga anntcttatn 480ttnaaatttc ttcccataaa nggcttgcca ccaangccat tatngcgngt gaagcaccac 540ctgacccata accctggaat aattntnnga aaaccggacc cttntaccna atcttttttc 600aggggnn 607 289 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 289actttttttt tttttttttt tttgagaatg aataagcagt tctttaatgg ttatttaaat 60atattccaga agagcgttta taattcattt acaagtgcag tattgcgcta gtaaatgtta 120cttgacctct tgtataaata atgccgatta agaattagtc ctggaatagt tttcgaattt 180ctaactctgt agatctaaaa cacaattgta aatggtataa agatgtaaga atcatattgt 240gataaagtca atctcaaaaa tagagaatcc agacccttcc cagataattt aagaactgag 300ttttcctcaa cttaaacatg atggccacac agaaaacagt aaagacactt ttcgatgtga 360tacaactgga taaaactcga gaatatgagt atttagngac caatgnatan acattantgg 420aattttaaaa ncccttttaa tctgaagccg aaaaaaangc cattttccaa gaattattgn 480gccctaatca tcatcnannc nngaatanna tncnttcccn ggatagnnnn nnntccncct 540tnggaaantg ggccnaantt ntttggtntn aaagggggnc cnttaantcc n 591 290 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 290ggacttggaa atggttgtct ggaaagcttc cactttggtc ttgacggcat tcaccctctc 60cagcaccttc tcctggattg ctaccccaaa atcatttcca tcttcaatct tggggatcag 120gtgttggatc catgtaatca ccagaatgca tttctctttg agagtccaga cttctggctt 180aaccagggca agcagggaca ggactttctc attcccaggg agaaatccac acttagggac 240ttctttcttc tcctgcttat ctgtttccat ctcatcatcc ttgggtggag ggtctgggat 300ggggatgtcc agtggggccc ggagggaagt caagtcagcc acattgaggg agtcctcttg 360caagagctga ttcaggtata tgattttctg tggcaagaat ctgtagagga attcctcanc 420ctnctggaaa agaatctgtc tgaagacctt cacctggttg cgggctttcc cgctaagcgc 480accccacacg gtttgggcct gctgntttaa tccttaanct ctggcttccg gntagtcccc 540cgggaccttg ccggccggcc ntcaaagggc aattcancna ctggcggccg tn 592 291 609 DNA Homo sapiens misc_feature (1)...(609) n = A,T,C or G 291acagtggcat gatctcggct cactgcaacc tctgcctccc gggttcaagc aattctcctg 60cctcagccac ccaagtagct gggactacag gtgcgtgcca ccacgcccag ctaaattttg 120tatttttagt ggagacgggg tttcaccatg ttggccagga tggtctcaat ctcctgaccc 180tgcgatctgc ccacctcagc ctcccaaagt gctgggatta caggcgtaag ccaccgggcc 240tggcctgttt tatgattctt aatagttact tggtttaaat cacatttgat actatccttc 300tgaaaagtct gagacagatc tacaaactac agtcaaaatt atagattaag aggaatgaat 360gcacctattt ggctttaagt tgaagatgaa ttatttctca tgctcatttt cttgcngcag 420ttatcttaga aagaccccca aaggcttgtg attgtaaagc acttgcatga tcacagaatg 480caagcttctg gtaccttcgg ccgtgacacg ctaagggcga attcatcaca attgcgggcc 540gtacctatgg atccannctc ggtccaactt ggcggaatca tgggcatact gnttcctggn 600nnaaatgtn 609 292 568 DNA Homo sapiens misc_feature (1)...(568) n = A,T,C or G 292actgcccaga aggagttcat aaagaataca aagaagaccc caaaagatgt cacgatggca 60ctattgaatt cacgagcatc gatgcacaca atggtgtggc cccatcaaga cgtggtgatt 120tggaaatact tggttattgc atgatccaat ggcttactgg ccatcttcct tgggaggata 180atttgaaaga tcctaaatat gttagagatt ccaaaattag atacagagaa aatattgcaa 240gtttgatgga caaatgtttt cctgagaaaa acaaaccagg tgaaattgcc aaatacatgg 300aaacagtgaa attactagac tacactgaaa aacctcttta tgaaaattta cgtgacattc 360ttttgcaagg actaaaaact ataggaagta agggtgatgg caaaatggac ctcaatggtg 420tggaaaatgg angnttgaaa gccaaaacca tnnnnnaaaa ncttagggcg aattccannc 480actggcggcc gtnctaangg atccagcttg gncccaactt ggggtaatca tgggcataac 540tggtncctgg ggaaaatggt ttcccnnn 568 293 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,C or G 293ggtacttttt tttttttttt tttttttttt tttttttctt tttttttttt tttttngcct 60ttttaanaaa cttttatttg agnggntntt acaaanattg nngcaatatg aaagtcattt 120gtttgatana aatatcaagc tgncttgtca aacacnctga agtaacccaa aaatntnttt 180caaagctcac anagcttaaa aagagcnaag attntntgca accagacaaa acctatttnt 240gcatttccta tttctttctn aaactgnttt gcctaccaaa ctttnacgtt taaacatttt 300caggaaatgc agggatcatt ttgtttggaa ttttaagacc ccccngaacn cataggtntt 360tacaaagaaa cttttcccga tcccttaatt gaaaagaacc ntccnaaata taaantttgn 420aaactcccnt ttttggccaa ttgatcanaa tgccagaaga natgctaacc naanagccct 480ttaactgggc tgggattcca taccctaaan ggggtttcaa aactggttaa ccttnnccca 540attttaacct tngggaaaag ggnaaaggan ccccggggna aaaataaggt tttgaaaaat 600aaa 603 294 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 294ggtacgcggg gatcgcttcc tggtcctcgc cccctccgct gtctccctgg agttcttgca 60agtcggccag gatgtctcag gctgagtttg agaaagctgc agaggaggtt aggcacctta 120agaccaagcc atcggatgag gagatgctgt tcatctatgg ccactacaaa caagcaactg 180tgggcgacat aaatacagaa cggcccggga tgttggactt cacgggcaag gccaagtggg 240atgcctggaa tgagctgaaa gggacttcca aggaagatgc catgaaagct tacatcaaca 300aaagtagaag agctaaagaa aaaatacggg atatganaga ctggatttgg ttactgtgcc 360atgtgtttat cctaaactga gacaatgcct tgtttttttc taataccgtg gatggtggga 420attcgggaaa ataaccagtt aaaccagcta ctcaaggctg ctcaccatac ggctctaaca 480gattaggggc taaaacgatt actgactttc cttgagtagt tttaatctga aatcaattaa 540aagtggattt tgtaccaaaa aaaaaaaaaa aaaaagtnct gcccggccgg ccntcaaaag 600gcnaattcan ccccttg 617 295 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 295cgaggtactt ttaccatgaa catctctaga ctgtgattat taaatatagt gataatatac 60atgggtttac tgggatattg aaaaataaaa gataatgaac ccaatttagt aaatcaacat 120aaatacaaaa cagagcgaat tagccctcta caactgagct cgtcctgcgt cttgagcttg 180ggttctttct ggaactgtct caaaccttag tgggggaagt gaccttatcc acagattgct 240tttcccagag gttccgcttg ctggatacgt ctcctggtct caagtcagaa ggtttgggag 300caggtgactt gtttccatct ggggttttag ttagccattc attgatgccg ctagaaaccc 360ctaccttcaa gccagcagtt tccttatttg gtgtgcctgc tgcantgggg gatgaaaaca 420cattcctttc tnccacatac tcttggatgt tgcgtacctg cccnggcngg ccgttcnaaa 480ggccaattcc acaccactgg cggccgtact aatggatcca aaactcggac cancttggcg 540natcatnggc atactggttc ctggggnaaa tggattccgt tacattcccc caacttccag 600ccnggg 606 296 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 296ggtacgcggg gtgccagagg aaatcttaaa gcgcctactt aaagaacagc acctctggga 60tgtagacctg ttggattcaa aagtgatcga aattctggac agccaaactg aaatttacca 120gtatgtccaa aacagtatgg cacctcatcc tgctcgagac tacgttgttt taagaacctg 180gaggactaat ttacccaaag gagcctgtgc ccttttacta acctctgtgg atcacgatcg 240cgcacctgtg gtgggtgtga gggttaatgt gctcttgtcc aggtatttga ttgaaccctg 300tgggccagga aaatccaaac tcacctacat gtgcagagtt gacttaaggg gccacatgcc 360anaatggtcc cgcaggaagg ccgtcaagaa nggctcgacc cggntggtgt ttcaaggaag 420aaacattgtg gtcttggtgt ggaaaaaaaa tcantgggcc aactggngga tgaaagacna 480tgccggaana nctgggcttt ggatgacaac ccctgcatgg gcttttgang ccttaccgcc 540gatccagggt tntnttaaca nggcccggtg gaatgccnaa nccccggtta ctttggagga 600cccggtnctt gg 612 297 590 DNA Homo sapiens misc_feature (1)...(590) n = A,T,C or G 297acgcggggga acacatccaa gcttaagacg gtgaggtcag cttcacattc tcaggaactc 60tccttctttg ggccacggaa ttaacccgag caggcatgga ggcctctgct ctcacctcat 120cagcagtgac cagtgtggcc aaagtggtca gggtggcctc tggctctgcc gtagttttgc 180ccctggccag gattgctaca gttgtgattg gaggagttgt ggctgtgccc atggtgctca 240gtgccatggg cttcactgcg gcgggaatcg cctcgtcctc catagcagcc aagatgatgt 300ccgcggcggc cattgccaat gggggtggaa ttgcctcggg caaccttgtg gctactctgc 360agtcactggg aacaactgga ctcttcngat tgaccaagtt catcctgggc ttcattgggt 420ctgccattgc ggctgcattg cnaggtctac taacttcctg cccttgcctt gcaaaaaaaa 480aaaccttgcc agggaaaaag nccccaancc ttctgaacca accanggggc ccacttttcc 540aaaatacctn gggnggaaaa tncccaattt tgantttcnn aggaaanana 590 298 590 DNA Homo sapiens misc_feature (1)...(590) n = A,T,C or G 298ggtactttga gccactctcg catggaaagg agtgtcttta tgcctcgacc tcaagctgtg 60ggctcttcca attatgcttc caccagtgcc ggactgaagt atcctggaag tggggctgac 120cttcctcctc cccaaagagc agctggagac agtggtgagg attcagacga cagtgattat 180gaaaatttga ttgaccctac agagccttct aatagtgaat actcacattc aaaggattct 240cgacccatgg cacatcccga cgaggacccc aggaacactc agacctccca gatttaacta 300aacaaaagaa actctccacc tagcactgtt tttcttcatt gcttactgag agggtttttg 360agaacttaat ctggggggag aactgctttc tcagatcctt aactcccgag aagagaagtc 420cttgtgcaca gaacttgtgg gaaccttcat ccgntgtctt tacctttgga tccagtgtgc 480aagtttcatg acngaatcat taagatatca aatggcctaa tttggngcna atcatggtat 540actgggaaaa ttaggcnaat ggaacttntc accgantttg gtctttaaan 590 299 549 DNA Homo sapiens misc_feature (1)...(549) n = A,T,C or G 299cgaggtacaa agatctgaca tgtcacccag ggacccattt cacccactgc tctgtttggc 60cgccagtctt ttgtctctct cttcagcaat ggtgaggcgg ataccctttc ctcggggaag 120agaaatccat ggtttgttgc ccttgccaat aacaaaaatg ttggaaagtc gagtggcaaa 180gctgttgcca ttggcatcct tcacgtgaac cacgtcaaaa gatccagggt gcctctctct 240gttggtgatc acaccaattc ttcctaggtt agcacctcca gtcaccatac acaggttacc 300agtgtcgaac ttgatgaaat cagtaatctt gccagtctct aaatcaatct gaatggtatc 360attcaccttg atgaggggat cggggtaacg gatggtgcgg gcatcatgag tcaccagatg 420anggattcct tttgtgccca caaagatctt tctactttgc ancacacact ggcggncgta 480ctagtggatc cacttcgnac caacttggcg tatcatgggc tnactggtnc cgggggaaat 540ggtatccnn 549 300 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 300actccagcct gggcgacaga gcaagactcc acctcaaaaa agaaatattt agcaaatatt 60aaaggacaag agggaatatc tgtttaaaaa attataatgc acgttagatg aaaagtaata 120ggatgagatg gttgttgctg aaatagcact tgctatataa attcaaacat tccttttcaa 180attcagcttc tcagaggttt gacttcagat gcttgagcac tttcaacatt atctttgcct 240ttatccttcn ttatgcggat aaacacaact gctaaaatta taccattgat tttggaaact 300tcccagtcgt tttgtaagct tcactgccga gggaaaatgt aaaatgggga ccccgaaata 360aagtgctgat catcatcaag tagcctcgaa aatgagactt tcaggtgcac tgaaggggat 420ggcagaagaa caagccccgt gtagtccttg ctagcctggg aaggttggca ttcacatcct 480taaggatcan gtggactttg acnccgaact taaaggaaga accccctatt ntggggccac 540cacttgacct tgggccggaa cacccttaag gcgaattcca cacactgggg g 591 301 655 DNA Homo sapiens misc_feature (1)...(655) n = A,T,C or G 301cgaggtactc tttaaaaagg gactgcaggg ctgggtgtag tggctcacac ctgtaatccc 60agcactttgg gaggccaagg caggtgggtc acttgaggcc aggagtttga gaccagcctg 120accaacatgg caaaacccca tctctactaa aatacaaaaa ttagctgggc atgatggtgc 180actcctgtaa tcccagctac ttggtaggct gaagcatgag aattgcttaa acctgggagg 240cagaggttgc agtaagccaa gatcatgcca ctgcactcca gcctgggcaa cagagtaaga 300ctctgtctta ataaataaat aagaaaataa aacggaactg cagtgctaac agtaatttat 360acatttttaa atgttctgag tatgttttga ctgggctagt gtaacaatat actaccctga 420aaagtgcagt tttgattgtt ggtggtgtct ttgggtcang aaaagtgaac tgtgccaaga 480agtatttttc aatgacatga atggattnct gttaatgcaa ttgactgaga aaatgngctt 540acgctttctt aactgcaaaa agagntttgt ccacatcana attgttgaaa ctggngctgt 600ttctgttgcc tgggatctga tgactgggat ttcctcttgg acaaaanacc tgatn 655 302 513 DNA Homo sapiens misc_feature (1)...(513) n = A,T,C or G 302actcgtcttg gtgagagcgt gagctgctga gatttgggag tctgcgctag gcccgcttgg 60agttctgagc cgatggaaga gttcactcat gtttgcaccc gcggtgatgc gtgcttttcg 120caagaacaag actctcggct atggagtccc catgttgatg gatcctgagc ttgaaaaaaa 180actgaaagag aataaaatat ctttagagtc ggaatatgag aaaatcaaag actccaagtt 240tgatgactgg aagaatattc gaggacccag gccttgggaa gatcctgacc tcctccaagg 300aagaaatcca gaaagcctta agactaagac aacttgactc tgctgatttt tttttccttt 360ttttttttta aataaaaata ctattaactg gacttcctaa tatatacttc tatcaagtgg 420aaaggaaatt ccaggcccat ggaaacttgg atatgggtaa attgatgacc aataatcttc 480acttaaagnc atgtcctttg gccgcgaaca cgc 513 303 610 DNA Homo sapiens misc_feature (1)...(610) n = A,T,C or G 303acgcggggct tgcagagccg gctccggagg agacgcacgc agctgacttt gtcttctccg 60cacgactgtt acagaggtct ccagagcctt ctctctcctg tgcaaaatgg caactcttaa 120ggaaaaactc attgcaccag ttgcggaaga agaggcanca gttccaaaca ataagatcac 180tgtagtgggt gttggacaag tnggtatggn gtgtgctatc agcattctgg gaaagtctct 240ggctgatgaa cttgctcttg tggatgtttt ggaagataag cttaaaggag aaatgatgga 300tctgcagcnt ggggagctta tttcttcana caccttnaaa ttgtgggcag atnaagatta 360ttctgtgacc cgtcaattct tanattngta gttggtnact gcatggaatt cngtcagcaa 420gaaangggaa aantctngtt caatttggtn gnataagaan tggttaatgg tcttcaaatt 480cnttattcct tcagancggc caagtacctn ggccgnganc atgcctaagg gctaattcna 540ctcantggng gccgntctan ntggattcca ncttggtacc aancttggng ntattnatgt 600caatanctgg 610 304 596 DNA Homo sapiens misc_feature (1)...(596) n = A,T,C or G 304ggtacctgga attatctaat tggccagagg tggcgcccga cccatcagtt cgaaatgtag 60aagtaataga gttggcaaaa gaatggaccc cagcaggaaa agcaaagcaa gagaattctg 120ctaagaagtt ttattctgaa tctgaggaag aggaggactc ttctgatagt agcagtgaca 180gtgagagtga atctggaaag tgaaaagtgg agaacaaggc cgaaagtggg ggaggaagga 240gacagcaatg aggacagcag tgangactcc tncagtgagc angacagtga gagtggacgg 300gagtcaggcc tagaaaacan angaacagcc nagangaact caaaagccaa agggaaaaag 360tgattctgaa gatggggaga aggaaaatga aaaatctaaa acttcagatt cttcaaatga 420cgaatctagt tcaattanaa gacagttctt ccgattcttg aatcagaatc agaacctgaa 480agtgaatctt gaatncngaa cagtcgctta ggagaaagaa agaaaccaag caggattgac 540tccttttncc aagntgttcc ttctaaactg gatgatttaa ccngntccct cagtgn 596 305 629 DNA Homo sapiens misc_feature (1)...(629) n = A,T,C or G 305ggtactttnt tttttttttt tttttttttt tttttttttt tggggattta ntttttattt 60cataatcata aacttaactn tgcaatccan ctaggcatgg gagggaacaa ggaaaacatg 120gaacccaaag ggaactgcag cgagagcnca aanattntng gatactgcga gcaaatgggg 180nggaggggng ctntcctgag ctacaaaagg aatgatctgg tggntaaaat aaaacacaag 240tcaaacttat tnnagttgtc cacagncagc aatggngatc ttcttgctgg ncttgccatt 300cctggaccca aagcgctcca tggcctccac aanattcatg ccttctttna ctttgccaaa 360caccacatgc ttgccatcca accactcant cttggnagng cagatgaaaa actgggaacc 420atttttnttg ggtccnacat ttccatggca aaangccang acccnttgct ttaagaagaa 480aatctcatct tcaaattctn ccctaaanga cttgccncan gccntntggg tgngaagcnc 540cccctgncca taaccctgga tatttttgaa agaggancct ntacnaacnt ttttccnggt 600aanaaaaaat tttttntttg acctnccca 629 306 643 DNA Homo sapiens misc_feature (1)...(643) n = A,T,C or G 306acagggagga atttgaagta gatagaaacc gacctggatt actccggtct gaactcagat 60cacgtaggac tttaatcgtt gaacaaacga acctttaata gcggctgcac catcgggatg 120tcctgatccc ccgcgtacat ttccttgtag actctgttaa tttcctgcag ctcctggttg 180gttctggagc agatgatctc aatgagagag tcctcgtcgg ttcccagccc cttcatggaa 240gcttttatct cagaagcgtc atactgagca ggtgtnttca ataggcccaa aatcaccgtc 300tccaggtggc cagataaggc tgacttcaat gctgatgcaa gntccttttt ggtccttctc 360tggtaggcga aggnaatatc ctgtctctgt ncattgcttg cggntgggca aaatgttgac 420aatggtgacc tcatccacac ctttggtctt tgatggntgg ntcaatgttc aaagcatccg 480ctcagcatca aaantaagta tangctttgc agacccatat gcacttgggg gngnngagng 540acaccctcca actgaacttg ccaggatttn tgaaagtaan anttttaaga acttgccgnc 600cccanactaa acnnccaatc tagcccnntn cctaacggcc aag 643 307 643 DNA Homo sapiens misc_feature (1)...(643) n = A,T,C or G 307cgaggtactt tttttttttt ttttttttnt ttnttnttnn tttggggatt nantttttat 60ttcataatca taaacttaac tctgcaatcc aactaggcat gggagggaac aaggaaaaca 120tggaacccaa agggaactgc ancgagagca caaanattct nggatactgc gancaaatgg 180ggnggagggg tgctctcctn agctacaaaa ggaatgatct ggtggttaan ataaaacaca 240agtcaaactt attcnagttn tccacagnca gcaaagggga ncttcttgnt gggcttgcca 300ttcctggacc caaaacgctc catggnctcc caaaatttat gccttttttt actttgccaa 360anaccacatg ctttgccttc caccnctcan tttttgnggg ggnaaataaa aancgggaac 420cnnttgtgtt tggnccnaca ttttccnttg gnaaaaaacc ncgacccctt tntttaagaa 480naaaatttta nttttaaaat tttcccctaa aaaggactgg cccnaaggcn ttttgggggn 540gaagcccncc ntccccnaaa cctggaaaaa ttttggaagc nggacccttt accaaatctt 600tntcctggtt aaaaaaaaat tttttttttt gacctttccc aan 643 308 653 DNA Homo sapiens misc_feature (1)...(653) n = A,T,C or G 308cgaggtacag agagtagctt ctgtgatgca agaatatact cagtcaggtg gtgttcgtcc 60atttggagtt tctttactta tttgtggttg gaatgaggga cgaccatatt tatttcagtc 120agatccatct ggagcttact ttgcctggaa agctacagca atgggaaaga actatgtgaa 180tgggaagact ttccttgaga aaagatataa tgaagatctg gaacttgaag atgccattca 240tacagccatc ttaaccctaa aggaaagctt tgaagggcaa atgacagagg ataacataga 300agttggaatc tgcaatgaag ctggatttag gaggcttact ccaactgaag ttaaggatta 360cttggctgcc atagcataac aatgaaagtg actgaaaaat ccagaatttc agataatcta 420tctacttaaa catgtttaaa agatggtttg tttgcaagac tttttgcata cttanttcta 480catgaattaa atcactggtt tnaaatgaca cttattaatc ctaataactg gtnaacccnc 540aaaaaaaaaa aaaaaaaaaa ntacttnccc ggcggccgtc gaanggcaat tcacncctgg 600cggccgtcta tggatccacc cggnccacct gggnaacagg cnactggttc tgg 653 309 649 DNA Homo sapiens misc_feature (1)...(649) n = A,T,C or G 309acttgcaaaa gcacttgaag tcattaaacc agctcatata ctgcaagaga aagaagaaca 60gcatcagttg gctgtcactg cataccttaa aaattcacga aaagagcacc agcggatcct 120ggctcgccgc cagacaattg aggagagaaa agagcgcctt gagagtctga atattcagcg 180tgagaaagaa gaattggaac agagggaagc tgaactccan aaagtgcgga aggctgagga 240agagaggctg cgccaggaag caaaggagag agagaaggag cgtatcttac aggaacatga 300acaaatcaaa aagaaaactg tccgagagcg tttggagcag atcaagaaaa cagaactggg 360tgccaaagca ttcaaagata ttgatattga agaccttgag gaaatggatc cagattttat 420catggctnaa cagggtgaac aactggagaa agaaaagaaa gaacttcaga acccttaaga 480atcagaaaag aagattgctn ttttgaagac ccacctttgg aaaaattcct ttgttaagag 540cctttcgagg acagaaaatt aagacatggt ctggggngcc cccgaggaga aagaattctc 600ctgcccttga cgtgaaaggt nttgcataaa atcatgtccn atcttgaga 649 310 319 DNA Homo sapiens 310cgaggtacta gccggacttg gattttctgg aaagatttca gttgaggaac gggaacaaag 60attatgatag ctttccgacc accaccaact tcaatttcct tagctgccgt aatattcagc 120tccctgagct gagccttgag gtccgagttc atctccagct ccagaagagc ctgggagatg 180ccggactcga actcgtccgg cttctcgcca ttgggcttca cgatcttggc gctcgaactg 240aacatggctt tctcctggga gaacttgccg agcgccggct taggaagaga ccccgcgtac 300ctgccgggcg ggcgctcga 319 311 646 DNA Homo sapiens misc_feature (1)...(646) n = A,T,C or G 311cgaggtactg atgcaacagt tgggtagcca atctgcagac agacactggc aacattgcgg 60acaccctcca ggaagcgaga atgcagagtt tcctctgtga tatcaagcac ttcggggttg 120tagatgctgc cattgtcgaa cacctgctgg atgaccagcc caaaggagaa gggggagatg 180ttgagcatgt tcagcagcgt ggcttcgctg gctcccactt tgtctccagt cttgatcaag 240ctgcacatca ctcangattt caatggtgcc cctggagatt ttagtggtga tacctaaagc 300ctggaaaaaa ggaggtcttn tntggcccca aaccaatgtt ctgggctggc caatgacttc 360acatggggca atggcaccaa caccggcaga acttgnaccc tattgccaca acatgtcctt 420atctnaatga nggncttctt tgtgaaaaca aaccccattc cccggaatta agnacaantt 480cttcaaactt gggtggnttc aagggcctcg atngcctgcc catatngggt ttttgccata 540aaacacaacn ttccnnaaag gaatccgant nttgttttgt tggancccat ttttgttccc 600aagaaaattn ggtaatatcc aaattgggga attaggaaaa tgggnt 646 312 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 312cgaggtactt ttgtgagagg gttcaatggg agagctttaa tgcagatgag acttgaagct 60tctgaagaag atctaagtct tgatgaggtt attcaaactc aaatcttgaa tgcataatga 120tgataggcca tggtcttcaa aaacgtggta cttttaatag caacagggtt tcaccatgtt 180ggccaggctg gtctcaaatt cctgacctca agtgatctgc ccacttaagt gctgggatta 240caggcatgag ccacaacatc tggccagaaa tattttttct tttctttctc tttctctctc 300tctttttttt tttttttttt tttggagctc gctctgtccc ccagctgcaa tgcaatgggg 360caatcttaac ttactgnaac ctccccttcc aggtcnaaag aatctttgng ctacctccta 420natntnggaa tacaagggcg tcccccacct actaattttg ntttttaaga aaaggagggt 480ttancatgtt ggtnngntga tcccaacctc cgaccttaan gancctccgc ctaatttcca 540aaggctggat nttggctgan cccaccccnc ttaaccaaaa ttnaaattct tttntcctgc 600cgggggcgtt aaagggaatc aa 622 313 674 DNA Homo sapiens misc_feature (1)...(674) n = A,T,C or G 313nggacttgaa atcattgaag ttctgcaaaa aggagatgga natgcacaca gaaagaaaga 60tacagaggtc cgcagacggg agctcctaga atccatttct ccagctttgt taanctacct 120gcntgaacac gcccaagacg tggtgctaga taagtcagcg tgtgngtagg tntctgncat 180tccngggaac agacnaattn gaccatnagg naacctgagc ttnccaaagt ncgcaaggct 240gaagaagana ggctnctcca ggaagccnac gagaaagana aangagccgt attttacncg 300aacatgaaca aatcaaaaaa naaaactgtc cgaaaaccgt ttggagcaaa ncaaanaaaa 360cagnacctgg gngcccaaag cattcnaana tatttgttat tancncaccn tgatggattc 420naaacnttat ttttncttgg cncggctggn ccgcccggct ngngnaaaga aaagaacttt 480nctaccnctc ccgaatcaag aaaagaanat ggcttttttn taaaanncaa cccttgggaa 540aaaattcttt gtttaananc cctccaangc ccgggaaatt aattcatgct ttgtgtgngc 600gaccnannaa aaaanaanan atccttcctt ccccttaann gaaaagggcc ttncaaaaaa 660tgattgccca agnc 674 314 646 DNA Homo sapiens misc_feature (1)...(646) n = A,T,C or G 314actttttttt tttttttttt tttttttttt tttgagatgg agtcttgctc tgtcgcccag 60gctggagtgc agtgttgcga tctcagctca ttgcaacctc tgcctcccag gttcaagtga 120ttctcctgcc tnagcctcct gagtagttgg gactacaggc acatgccacc atgcctggct 180aatttttttg catttttaag tanagacagg gtttcatcat gttggccagg caggtntcaa 240actcctgacc tcaagtgatc cacctgtctc agcctcccaa agtgctggga ttacaggcat 300gagccactgn acccggccta aaaatgatta cttcttataa aaaggatttc ttccccttca 360caacacttan cttccttttt ctttcctggn aactatgggt ntggngnccg cataaggatc 420taccttncnc aagctggaca ntgggggttg ctncttgang gnaactcagg ccanatacng 480accctggggg gaacnctaaa cttacttggg tanaacccgg gctaacattt ctgcttgnga 540ngttgattcc ccncaaattt ttaaaaggnn tttcatggcc cttagggcaa ccattttaca 600aagatgggnc acatggnctt ggccgnaacc cctangngaa ttcncn 646 315 666 DNA Homo sapiens misc_feature (1)...(666) n = A,T,C or G 315acagtctttg gatatttagg aaggggatgg ggagaaagtc agttctcaga acaaattagt 60cagcttcagt ctcgtcagca gggtctttgg attctttgtt cttccgcact tcttcaatgt 120gcttatcctt ctctcgcaaa cgttccagtt tggcagccat ttgtgcctct cggttctctt 180tattagcttc cattttgtgg gtcagtttct cttctgccat tttactgaag ttgntgttct 240cttctattgc cttctgaagc acttctttct cgtgctctcg tttctcancc agctgcttca 300agaccttagc ttcatgggac ttgcgtcttt cttctgcagc ttctaatttc ttctgaattt 360cctccaggga aagaccttct tctttggaag ggaaaggggg aattctggaa ccagattctt 420ttgacccaag gctgaaaatc agcttaaaag cctggccttg angcacccnt tttcagntct 480ttcacctgga tatcntaaag aagccctngt gattnaaaac aagccnaccg gcantnnatt 540ntgncaanan cnnggataan gnaatccctg tnaantccna cccctnaccc cattttcccg 600ggaccttggc ngnaacccct tanggngaat tcnnccnctn ggcggccgta ctaangggac 660ccaccg 666 316 656 DNA Homo sapiens misc_feature (1)...(656) n = A,T,C or G 316actcttggtt tgtcaatggg actttccagc aatccaccca agagctcttt atccccaaca 60tcactgtgaa taatagtgga tcctatacgt gccaagccca taactcagac actggcctca 120ataggaccac agtcacgacg atcacagtct atgcagagcc acccaaaccc ttcatcacca 180gcaacaactc caaccccgtg gaggatgagg atgctgtagc cttaacctgt gaacctgaga 240ttcagaacac aacctactgt gggtgggtaa ataatcagag ccttcccgnc aagtcccagg 300cttgcagctg gccnatgacc aacaggaccc tnactctact tagtgtcaca aggaatgatg 360ganggaccct atgaagtgtg gaaaccagaa ccaattaagt ggtgnccaca cganccaggc 420attcttgaat ggcccttatg gnccanaaga acccaccatt tcccctnata cacctaatnc 480cgtccagggt gaaccttaag ctntctggca tgcaancctt aaccactggc aggattcttg 540gnttaatgaa gggaacattc nnacccnccc agaagttttt attttcaact tacttggaan 600aacgggggct ntttactgcc ngccataact taacnggggc cnnancggac ttcgnn 656 317 636 DNA Homo sapiens misc_feature (1)...(636) n = A,T,C or G 317actttttttt tttttttttt tttttttttt tttgnagtca gctatttaat taggttctta 60agacatttag aacaccaatt tgngaggata aattccattc gtcagagcaa acacagatcg 120caggtagccc tggagctgag gaatagcttt gatttttggt aaaatttgtg agtccacagc 180tttctgatca atcttgcgct gctccgtaat ctcatatttc cctttttctg ggncgaaaan 240cttacctttc tggggnntgg gcttncgcag cttcttcttn ttgaagtaag catnagtaan 300aagntttggg anttttacan tgntgatann cattttggnt gaagnggnan tgacnaattt 360ctgggggggt cttcgtaaag gaactcnant gaggcccaag ggnccgtccn agtaataagg 420ccctnncanc tggttangga aaccccctnt tggcctgggg ggnccangag gntgatccaa 480atggccccgg ggaaaagcng gntcaanttt tnacggctnc tnaaagggtt ttgccnggnt 540taanctttnn ggncnttttc agnggaaana ccngctttgn nantntaccc ccggntcctc 600ggcggaaacc nttagggnna attncncnct gggggg 636 318 654 DNA Homo sapiens misc_feature (1)...(654) n = A,T,C or G 318cgaggtacgc ggggcctttc tgcccgtgga cgccgccgaa gaagcatcgt taaagtctct 60cttcaccctg ccgtcatgtc taagtcagag tctcctaaag agcccgaaca gctgaggaag 120ctcttcattg gagggttgag ctttgaaaca actgatgaga gcctgaggag ccattttgag 180caatggggaa cgctcacgga ctgtgtggta atgagagatc caaacaccaa gcgctncagg 240ggctttgggt ttgtcacata tgccactgtg gaagaggtgg atgcagctat gaatgcaagg 300ncacacaagg tggatggaag aattgtggaa ccaaagaaaa ctgtcttcag agaagattct 360taaagaccan gtgcccactt aactgtgaaa aagatatttg gtggtggcat taaagaagac 420actgaagaac atcactaaga gantattttg aacagtatgg anaaaattgn agngattgaa 480atnatgactg ccnangcagt ggcancaaan ggggctttgg ctttnnacct ttgacnacca 540tgactcnngg ataaaatggn attcnnaaat ccctcntgng aatggccnca ctgggaagtt 600ngaaancctn ncaacnagaa agggtncgnt tnntccncca aangcnaang tttc 654 319 659 DNA Homo sapiens misc_feature (1)...(659) n = A,T,C or G 319acgcggggaa gccaactcag actcagccaa cagagattgt tgatttgcct cttaagcaag 60agattcattg cagctcagca tggctcagac cagctcatac ttcatgctga tctcctgcct 120gatgtttctg tctcagagcc aaggccaaga ggcccagaca gagttgcccc aggcccggat 180cagctgccca gaaggcacca atgcctatcg ctcctactgc tactacttta atgaaagacc 240gtgagacctg ggttgatgca anatctctat tgncagaaca tgaattnngg caacctggtg 300tctgtgctna cccangccca aggtgccctt ggggcctcac tgattaanga aantggcact 360gatgacttca atggctggaa tggccttcat gaccccnaaa aagaacccgc gnttgcactg 420gacagtgggt ccctngntct cttacaagtc tggggcaatt gganccccaa nccatgntaa 480ttcnggctac tggggtgagc nnacctcagc ccaggatttn gaantggaan gcctgncttg 540ggaanacaag ttcttctttn gctngcaagt tcaaaaccta atgcagctgg aaaatcatnt 600ctanaactga tcagcattcn accgnttcaa attaaccggc ctttttcant tanttaccg 659 320 664 DNA Homo sapiens misc_feature (1)...(664) n = A,T,C or G 320ggtactctgc cttttaggag atgaggtaag acatatacat agatggcttt tactagccaa 60ggcaatgtaa atggactaag attctcatgt gacttgaggt tatctgatga atttattctc 120ttcaaaacca cctactttta gagggcatgt ttaacccctc tctttattta aggagggaga 180gaaaaacaca tgtaaccaga attcagagtg ggttactcaa cctaagagaa catacggagt 240tctctttggg aaaacgacaa gactacagtg ttcacttcgc accatgaagt ggcactcctg 300ntatggctgc agantcctct tacttcttat gaaaggatgc atctgattct gaaattactg 360atatattcga tcagttaggg atgntttaaa aagngaaaac caatgccaca catacacttt 420ctagctttct gaaaatnacc cgacacattn ccnaaaatng agaatttacc ctattacttt 480tagagaaatt tccataatat tcttgggtaa agaancccng ttgggcatat tnccaatttt 540cagnggncnt ggttttatgc ccnaganccc aataggntcc cccatttttt aaggcttttt 600ccacngacga ttttttaaan cnttctnnan tgggggaaga ataatcttaa aagtngnctt 660atnt 664 321 666 DNA Homo sapiens misc_feature (1)...(666) n = A,T,C or G 321cgaggtacag tattacagtc agccacagaa gctgtgttgg gggacaagac ccaatccttc 60cccacaccag gcaaagcagt attggacatg agttggcatg tggctgggcc cacgtcctta 120tcccccaggc ctgaggggag accaccttct gatgataacc aacccctagc taccactctg 180tattcatcag gggaggggta taaaccccgc atgcaagaag aacccttgcc cccagtgtca 240aatgggatgg ggatgctaga gttatagtaa aggggaaacc ctatgtaagc tgntaacaga 300gttcacaggg gtagggataa cccctgntct tcagctncca aatgngctca ctttccagct 360tcttcatccg tcatcaatgc tggcaaagtt tccctnaact gnggccaggt tttcacgcat 420gggtggctgc acctgggtca aaaaggtggn attggccntt aaggaattag caatcatntg 480ctgggtggga ttccagtgtg taaggaactt anccaactgc atggnttgnt tgtgcanctg 540cttgatggng acaagtttnt gcaccanctn aaggaaggtg gaagcatggg gctcaacctn 600gataagttca tatacttggg gcnccttgct ttgggatctg catntttaca aggnttatcn 660tggcan 666 322 631 DNA Homo sapiens misc_feature (1)...(631) n = A,T,C or G 322accggaaagg aagctcccat tcaaaggaaa tttatcttaa gatactgtaa atgatactaa 60ttttttgtcc atttgaaata tataagttgt gctataacaa atcatcctgt caagtgtaac 120cactgtccac gtagttgaac ttctgggatc aagaaagtct atttaaattg attcccatca 180taactggtgg ggtacatcta actcaactgt gaaaagacac atcacacaat caccttgctg 240ctgattacac ggcctggggt ctctgccttc tccccttacc cttccggctc cacccttcct 300gcaacaacag ccctntacct ggggggcttg ntagaagaga tgtgaagggt tcaaggtcgc 360aacctgtggg actactgcta ggtgtgtggg gnggttcgcc tgcacccctg ggttctttaa 420gncttaaagt gatgcccctt tccaaccatt attctggncc cacacttctc actccggcct 480tggncnanca taaatgnacc ccttcacttc ctntgagaat ggccttcgng aagaatcnag 540gctttcccaa ncttctttcc ccccnttatc angggngctg gttttctnct ctcnaaggtc 600ntttgaccgn accaccaaac ttctgaattn t 631 323 647 DNA Homo sapiens misc_feature (1)...(647) n = A,T,C or G 323actgtgggtc gaagtaatgg atacggacgt aaccatcttc gccgccgctg ctgtagctct 60tgccatcagg atggaaggca acactgttga taggtccaaa gtgacccttg actcttccaa 120actcttcttc aaaggccaaa tggaagaacc tggcctcaaa cttgccaatc ctggtggagg 180ttgtggttac atccatggct tcctgaccac cgcccaggac cacatggtca tagttggggg 240agagggcagc tgagttgaca ggacgttctg tccggaaagt cttctgatgt tcaagagttg 300tggagtcgaa aagcttggct gtgttgtcct tggacgcggt cacaaacatg ggcatgtccc 360tggataactg gatgtccgtg atctgcccgg agtgcttctt aacattncca acacctnttc 420aaanttggca ctatactggg tgagctcttc acttttatng gcaacgnatg atcacttccc 480caagggtccc caaacagcac tggggaattt agagncattc cagggaactt tatgtagggt 540tcatggtgca attggttnga tccccaggtc aaaaagttnc aaacactgga nccctttctt 600gtccnnggag aacatgttat ttgccccaag taaaacccng nccggng 647 324 653 DNA Homo sapiens misc_feature (1)...(653) n = A,T,C or G 324ggtacttttt tttttttttt tttttttttt ttgagatgga gtcttgctct gttgccagac 60cggagtgcag tggtgcgatc tgggctcact gcaatctcca cctcccgggt tcaagcgatt 120ctcctgcctc agcctcccga gtaactggga ctacaggtgt gcgccaccaa gcccagctca 180tttttgtatt tttagtanag atggggtttc acgatgttgg ctaggatggt ctcgatctct 240ggtcagagtc ttttctgtaa aaatccttgg taaagaagca attttagact gtancctgtt 300gcaaatgcnt taaggaagaa gcaaaacaac tgntagtctt tctgaaatga aaaaactacn 360ccagggctgg tatatnnaga gcaaccccaa ccannactnc catcntgatg cccacagggg 420cccactgana nacccngaaa angtccnnaa gcntaaannt ngangcnttg cttttgaaat 480attgcgccng taccnagntn nagacaaacn ngnttaaggc ccnnantntt tggccngant 540ttgcgataaa aaaaacttgg gggtcgctnc nngatcccnn ttgtncccca naanctgggg 600ggatgggttn aagcccntgn cnnaaggttt nngttctccc aaggtaaaan nng 653 325 655 DNA Homo sapiens misc_feature (1)...(655) n = A,T,C or G 325ggtacgcggg gccttttggc tctctgacca gcaccatggc ggttggcaag aacaagcgcc 60ttacgaaagg cggcaaaaag ggagccaaga agaaagtggt tgatccattt tctaagaaag 120attggtatga tgtgaaagca cctgctatgt tcaatataag aaatattgga aagacgctcg 180tcaccaggac ccaaggaacc aaaattgcat ctgatggtct caaggggtcg tgtgtttgaa 240agtgagtctt gctgatttgc agaatgatga agttgcattt agaaaattca agctgattac 300tgaagatgtt caagggtaaa aactgnctga ctaacttcca tggcatggat cttacccgtg 360acaaaatgtg gtccatgggc aaaaaatggc agaccatgat tgaagcttac ggtgatgtca 420agactaccga atgggtactt gcttcgtctg gtctggggtg ggtttactaa aaaacgcaca 480atnanatacc gaagaactct tatgcttang accacangtc cngccaatcc ggagaanata 540tggaaatctg accccaaagn gccnaccaat gacttgaaaa annggccatt aaatggttcn 600nacacnttgg aaaagcctta aaaggttgcc aantattaac ctntcatgaa gnttc 655 326 657 DNA Homo sapiens misc_feature (1)...(657) n = A,T,C or G 326ggtacgcggg ggaaacggga gtgaacggag agcgtagtga ccatcatgag cctcctcaac 60aagcccaaga gtgagatgac cccagaggag ctgcagaagc gagaggagga ggaatttaac 120accggtccac tctctgtgct cacacagtca gtcaagaaca atacccaagt gctcatcaac 180tgccgcaaca ataagaaact cctgggccgc gtgaaggcct tcgataggca ctgcaacatg 240gtgctggaga acgtgaagga gatgtggact gaggtacttt tttttttttt ttnttctttt 300ttttgagata gggnctcact gnatnaccca ntntggaatg caattggcat gaacncagct 360tactgnagnc ttccaaacct gggctcaagc aattatnttg nattaacctn ttgagtacct 420gggactntcn cangcaccan ccctgctttg cttacttaaa tttttgtnaa nacnnggctt 480gctttttttc ccaggntggn tcnaactccn gaattaaggg atccttcccc ctcaattttt 540aaannngctg ngattntnga atangccttt ttgttngccc ttttnacctt ttnnnnggtt 600nnttcnnggc tttaancctn ccgggggccn tttaaaggng aaatcncncc ttggggg 657 327 658 DNA Homo sapiens misc_feature (1)...(658) n = A,T,C or G 327ggtacttttt tttttttttt tttttttttt ttttttttgg tttgaaacag aaatttattc 60tcanaataat gcacagaagc acaggttgag gctactcttg ggaagcttcc ctccccttcc 120tcttcctcct ctccctcctc tctgaatgcc agggagaaca cagttgaagg aaggaaacat 180gcaatcacaa acaatgaaca actntaaaga caaaaaggtt tggtccaaaa gaactcaaca 240taattaatcc aatgactgtg aanagcttca ctgagtagga ttaanatatt gcagatgtan 300ngtttncaca gggtggctnt tcagtgcacc ancggggcct ncttgangga natgaggact 360gacncatncg ggaaanatct ttggcctgct tgctaaactt ggggntaaag gcacacnnnc 420cgggccaccc gttccactna nngcctgggg accanttgtc aatgncnttt ccnaangntt 480tttttgntgc cttgtggttg nttttggttt ctggaactgn tcgncctgnc ttgnaaacca 540ttnttntaac nccttaatgg cctttctttt cnnnctggtt ntgnttccaa aatnggatta 600ngggttcang ngcccctact tnccgggggc ngttaaangg naattccncc nctggngg 658 328 644 DNA Homo sapiens misc_feature (1)...(644) n = A,T,C or G 328acgcggacgg tggtttttgg gcccgtttct gagcagcgct tcctttttgt ccgacatctt 60gacgaggctg cggtgtctgc tgctattctc cgagcttcgc aatgccgcct aaggacgaca 120agaagaagaa ggacgctgga aagtcggcca agaaagacaa agacccagtg aacaaatccg 180ggggcaaggc caaaaagaag aagtggtcca aaggcaaagt tcgggacaag ctcaataact 240tagtcttgnt tgacaaagct acctatgata aactctgtaa ggaagttccc aactataaac 300ttataacccc agctgtggnc tcttgagaga ctgaagattc naggctncct ggccagggca 360gccctttagg agcttcttag taaaggactt atnaactggt tttnaancac agacctcaag 420taattnacac cagaaatncc nnggtggaga atnctccnct gctggtnnag angcatgaat 480aggnncaacc agctntctct gnccnnaccn cncttaggnc naattccgca ccctgcggcc 540gttctnatgg atccnaactn ggtnccaant nggcnnacta tggcatancn tgccctgggg 600aantggttcc nttccaatcc anaanttcta tcgnaactta acgg 644 329 651 DNA Homo sapiens misc_feature (1)...(651) n = A,T,C or G 329actattagcc atggtcaacc ccaccgtgtt cttcgacatt gccgtcgacg gcgagccctt 60gggccgcgtc tcctttgagc tgtttgcaga caaggtccca aagacagcag aaaattttcg 120tgctctgagc actggagaga aaggatttgg ttataagggt tcctgctttc acagaattat 180tccagggttt atgtgtcagg gtggtgactt cacacgccat aatggcactg gtggcaagtc 240catctatggg gagaaatttg aagatgagaa cttcatccta aagcatacgg gtcctggcat 300cttgtccatg gcaaatgctg gacccaacac aaatggttcc cagtttttca tctgcactgc 360caagactgag tggttggatg gcaagcatgt ggtgtttggc aaagtgaaaa gaaagggcat 420gaatattgtg gaggccatgg aaccgctttg ggtccaggaa tgncnagaac agcaagaaga 480acaccattgc tgactgngga caactcgaat aagttggact tggggttant ttaaccacca 540gaacaattcc tttgtncnta aggagancan ccctcaccca tttgntngca tatcctanaa 600actttgggct ttcnttngtt cctttggttc aggtttcctg gtcctccanc c 651 330 643 DNA Homo sapiens misc_feature (1)...(643) n = A,T,C or G 330actttntttt ttttntnttt tttttttttt ctggaaggnt ctcaggtctt tatttgctnt 60ctcaaattcc aggaatngac ttatttaatt aatccatcaa cctctcatag caaatatttg 120agaaaacaaa tttatattca gattcttatt ttcagtaggg aagtaagaag ttgcagctca 180ttgcacgtaa agttgagaca ganatggaga catccagccc cacctntctg gaacaagaaa 240gatgactggg gaggaaacac aggtcancat gggaacaggg gttacagtgg acacaagggn 300gggctgnctn ttcacctnct tacattatgc taacagggac ncaaacccat tcaggggcct 360ttgcnaaaag aaatgccaaa agctnttgaa gtcncnaagg ggangcgtga anaaaactgc 420atttnagtcc ccgggtcctt ngncgggaac ccctnanggn gaaatcncca cactggcggg 480ccgtactagn ggatccagct nggncccaat tggnggaata tggnnaanac tgttcctgtg 540ggaaaatggn atccgtccaa ttcnccactt acanncggag cctaaangna aaacntgggg 600ngcctatggg gggctacnnn aataatgggt gcctacggcc cnt 643 331 652 DNA Homo sapiens misc_feature (1)...(652) n = A,T,C or G 331ggtacagatg gcactgacaa tcccctttct ggtggggatc agtatcagaa catcacagtg 60cacagacatc tgatgctacc agattttgat ttgctggagg acactgaaag caaaatccaa 120ccaggttctc aacaggctga cttcctggat gcactaatcg tgagcatgga tgtgattcaa 180catgaaacaa taggaaagaa gtttgagaag aggcatattg aaatattcac tgacctcagc 240agcccgattc agcaaaagtc agctggatat tataattcat agcttgaaga aatgtgacat 300ctccctgcaa ttcttcttgc ctttctcact tggcaaggaa gaaggaagtg gggacagang 360agatggccct ttcgcttang tggccatggg ccttnctttt cactaaaagg aattaccgaa 420cagcanaaag aaagncttga gatagtgaaa atggggatga tatctttaga agggngaaga 480tggggtggat gaaattattc attcctgnga agnttgnaaa ctgngcgnct tcnnnaaant 540nnnaggcatt ccntnnctgg ccntgccatt gccattggnt ccanttgcta tagggatgcc 600ccttaaancn ntttccnnna anagtnnaaa acttgcnntn ggatccaacc nn 652 332 648 DNA Homo sapiens misc_feature (1)...(648) n = A,T,C or G 332cgaggtactt tttttttttt tttttttttt tttttttgag acagagtttc actcttgtcg 60cccaggctgg agtgcagtgg cgcgatctcg gtccactgca acctcaccct cccaggatca 120agcgattctc ctgcctcagc cacctgagta gctgggatta caggcgcctg ccactacacc 180tagccaattt ttgtattttt agaagggaca gcatttcacc atgttggcca ggctggtctc 240gaactcctga tctcaggnga tccacccacc tcagcctccc aaagtggngg gattacaggc 300gtgagccact gaaagttctc attagttttt tggttaaatt ttaaacataa attatgttat 360agcaaaaatt cctaagaatt gnaaaccact ttatcagaaa tatcnnaaat tcacaaataa 420tnccaaaatt tataatagct tttttccaga ctaaaatttt aaagctactg anaagnggna 480aacctnccta nataggattt acctaacatt nnggantaaa aggnanccan ngcctgctaa 540anatccagan tatctaanaa tccntncctg nntctcnntc tatnttttca natccgaatt 600tttgaaccca cnttangata nctnntttcc cccttaacnn taattccc 648 333 656 DNA Homo sapiens misc_feature (1)...(656) n = A,T,C or G 333cgaggtacaa gatgtccaaa tattgcgaag atctatttgg ggatctcctg ttgaaacaag 60cacttgaatc acatccactt gaaccaggca gggctttgcc atcccccaat gacctcaaaa 120gaaaaatact cataaaaaac aagcggctga aacctgaagt tgaaaaaaac agctggaagc 180tttgagaagc atgatggaag ctggagaatc tgcctcccca gcaaacatct tagaggacga 240taatgaagag gagatcgaaa gtgctgacca agaggaggaa gctcaccccg aattcaaatt 300tggaaatgaa ctttctgctg atgacttggg tcacaaggaa gctgttgcaa atagcgtcaa 360gaaggcttca gatgaccttg aacatgaaaa caacaaaaag ggcctggtca ctgtagaaga 420tgagcangcg tggatggcat cttataaata tgtaggtgct ccactaatat ccatncatat 480ttgtcaccat gatcaactac cccagnctgt naaggttcaa ggtttcatgt ggcanaagaa 540ccccatattc ttttacatgg cttctttaat gaatcatcgg cttggtactg aaaccctgcc 600attgaattgc attntacaac ggcaatgagc natttcccca gggaggccng cnttct 656 334 647 DNA Homo sapiens misc_feature (1)...(647) n = A,T,C or G 334acgcgggcgg gaagtgcaga ggcaaatgca tttagtgttc ttcagcatgt cctcggtgct 60gggccacatg tcaagagggg cagcaacacc gccagccatc tgcactaggc tgttgccaag 120gcaactcagc agccatttga tgtttctgca tttaatgcca gttactcaga ttctggactc 180tttgggattt atactatctc ccaggccaca gctgctggag atgttatcaa ggctgcctat 240aatcaagtaa aaacaatagc tcaaggaaac ctttccaaca cagatgtcaa gctgccaaga 300acaagctgaa agctggatac ctaatgtcaa tggagtcttc tgagtgnttc ctggaagaaa 360gtcgggtccc aagctctaag tgctggntct tacatgccac cattcacaag tctttaacag 420aatgattcan tggctaatgc tgatatcata aatgcgnaaa naaagtttgg ttctggcnag 480aagtcaatgg cancaagtgg naaatttggg acatacncnt ttgtgataag tggaatactg 540gngcncnctt acngganana cttaacgttn tttaanccaa acacaaccct tgaaagnnna 600agctctaaan accattggct tttttcnggg ngnaaaaaag gcttaag 647 335 657 DNA Homo sapiens misc_feature (1)...(657) n = A,T,C or G 335acaggtcaga gtcttctttt cttttctttt tgagatggag tcttgctctg ttgccagact 60ggagtgcagt ggtgcgatct gggctcactg caatctccac ctcccgggtt caagcgattc 120tcctgcctca gcctcccgag taactgggac tacaggtgcg cgccaccaag cccagctcat 180ttttgtattt ttagtagaga tggggtttca cgatgttggc taggatggtc tcgatctctg 240gtcagagtct tttctgtaaa tatccttggt aaagaagcaa ttttagactg tagctgttgc 300aaatgcttta aggaagaagc aaaacaactg tcaagtcttc ctgaaatgaa gaaactacac 360cagggctgct atatcagagc aaccccaacc agcacttcaa tcatgatgcc nacagtggcc 420cagctgagag accnggagaa agttccagat gcanagactg ngatgctctt gactatggaa 480atattgcggc cagtaccaag ttagagacca aacaggcata ngnncccgta ttaattggcg 540tgaattttgc gataaganaa cttggggggg tgctgcggat nccatgatcn ccagaaaact 600tnngggatgg ggtanaggcc catggcagaa aggttanggt ccttccaaag naaaana 657 336 649 DNA Homo sapiens misc_feature (1)...(649) n = A,T,C or G 336ggtacgcggg caactatgga attccacagc gtgctctgcg gggtcactcc cactttgtta 60gtgatgtggt tatctcctca gatggccagt ttgccctctc aggctcctgg gatggaaccc 120tgcgcctctg ggatctcaca acgggcacca ccacgaggcg atttgtgggc cataccaagg 180atgtgctgag tgtggccttc tcctctgaca accggcagat tgtctctgga tctcgagata 240aaaccatcaa gctatggaat accctgggtg tgtgcaaata cactgtcagg atgaaaacca 300cttaaantgg gtgncttgng ncccttntng cccaacagca acaaccctat tatcgtcttc 360tgnggctggg acaaactggn taaaggatgg aacctggcta actgnaagct gaaaaacaac 420cacattgggc acacangcta tntgaacacc gngactggct ttttcagang gatcctntgn 480gcttntggag gcaaggatgg gcaagccatg ttatnggaac tcnaccaang caacaccttt 540cacctttaan ggggggacat tatnaacgcc ttgggttaac cttaacgttn ttggtttgng 600ctgcncaggc ccacattaaa aatgggattt aanggaaana catttnann 649 337 652 DNA Homo sapiens misc_feature (1)...(652) n = A,T,C or G 337actcttggtt tgtcaatggg actttccagc aatccaccca agagctcttt atccccaaca 60tcactgtgaa taatagtgga tcctatacgt gccaagccca taactcagac actggcctca 120ataggaccac agtcacgacg atcacagtct atgcagagcc acccaaaccc ttcatcacca 180gcaacaactc caaccccgtg gaggatgagg atgctgtagc cttaacctgt gaacctgaga 240ttcagaacac aacctacctg tggtgggtaa ataatcaaga gccttccggt cagtcccagg 300ctgcagctgt caatgacaca ggaccctnac tctactcagt gtcacaagga atgatgnaag 360gaccctatga atgtggaatc cagaacgaat taaagcgttg accacagcga ccangcatcc 420tgaatgcctt tttgggccan acgaccccac cattttcccc tcataccact attaccgtcc 480aggggtgnac cttagncttt tcttgccatg cagcctttac ccaccttgac agnattcctg 540gctggatgtt gggaacatna gnacncacnc aagagctntt ttttccaaca tnatgggaaa 600acanngnnct tatactgcag gccattactt ngccntngcc cagnnggctn cg 652 338 651 DNA Homo sapiens misc_feature (1)...(651) n = A,T,C or G 338ggtacatttg aacacacggc tgtgttaaag atgctgctaa tgtcagtcac tgggtgcact 60aaaggatctc ttattttatg taaaacgttg ggattgacaa gatagatctg acactctgtt 120aagttaccct ctgaagctac ttcttgtgaa atactaatga cagcatcatc ctgccaagcg 180aaagaggcag gcataagcaa ggacaaatta aaagggggta agagccttat catgatgagg 240agtcttgntt tgacatcttg ggaaaagctg ccatagtgtg aaagtcgtca atttctcacc 300atggtttgca gtttgactgn ctctagttag ggtgaagtct ctgagtggca cacaccttaa 360gcctgaaggn tttcccttta aattttcatt gagttggccc tcttcagcat atanggcttt 420aagaacagaa canaccttgg ttttaagtgg gtccatggga taaaatggga atggangact 480ngaagaattc aagggctggg ccatctngca gtattctgaa tatcgaaaat ncnccaaggc 540tgctatataa anccccctgg gcaanacttc aatcggaanc ccacggnggc ccnactnana 600gncaggaccn ttccaantgg aacatnggan tggggccttt gaggcnnggn n 651 339 634 DNA Homo sapiens misc_feature (1)...(634) n = A,T,C or G 339actttttttt tttttttttt ttttttctag tttcagttat ttattgattt aatcattgta 60atctccaata gagattacaa tagagatctc caacatgatt tcatgcattt agaggagaaa 120tatttcctgg ttaagtggaa aattgtgcgg atgtggcttc tggaanacct tcattctaaa 180gcagcgttat agtgaaacat ttcatttana aatctggacg ttccttcttc agcttgctgt 240aatccacatt cactgagtag aacttgtatt gatcattggg acccagtttg ttccagggct 300ctgggttatt tctgcccaac aaacatctgg attgaacaat gccagacgca agagatcagt 360gttgctccag tagctccagt tccaataaat acnaagaggg ggatcaaagc tcggatgctt 420cttggcctga ccgatgatct ggcggancat gtttgcngca aagtctccga ctggaaagga 480ganaaccgcc taccccaagc cctaagctaa aaattatntg ccccgcgacc ttggncgnga 540cccnctaagg caattccacc actggcggcc gtctaangga tccacttggg ccaacttgng 600naacatggca nactggtcct ggggaangta tccc 634 340 655 DNA Homo sapiens misc_feature (1)...(655) n = A,T,C or G 340ggtactcttc cactcaggta tccgtgcggc cactccagca cacgcagtat gagcgcttca 60tcccctcggc ctacccctac tacgccagcg ccttctccat gatgctgggg ctcttcatct 120tcagcatcgt cttcttgcac atgaaggaga aggagaagtc cgactgaggg gctagagccc 180tctccgcaca gcgtggagac ggggcaggga ggggggttat taggattggt ggttttgttt 240tgctttgttt aaagccgtgg gaaaatggca caactttacc tctgtgggag atgcaacact 300gagagccaag gggtgggagt tgagataatt tttatataaa agaagttttt ccactttgaa 360ttgctaaaag tggnattttt cctatgtgca gtcactcctc tcatttctaa aatagggacg 420tggccaggca ccgtggctca tgcctgtaat ccacactttt ggaggncnng caagcggtta 480cgaagtcagg agatcgagac tattctggtt acacgnaaaa cctgncttac taaaagtacc 540tgcccggccg gccgntcaaa ggcgaatcca cacactggcg ggcgtactan tggatnccaa 600cttggaccaa cttggngnaa tatggcatac tggttcctgg nggaaatggt accnn 655 341 648 DNA Homo sapiens misc_feature (1)...(648) n = A,T,C or G 341acgaacctac agttttaact gtggatattg ttacgtagcc taaggctcct gttttgcaca 60gccaaattta aaactgttgg aatggatttt tctttaactg ccgtaattta actttctggg 120ttgcctttgt ttttggcgtg gctgacttac atcatgtgtt ggggaagggc ctgcccagtt 180gcactcaggt gacatcctcc agatagtgta gctgaggagg cacctacact cacctgcact 240aacagagtgg ccgtcctaac ctcgggcctg ctgcgcagac gtccatcacg ttagctgtcc 300cacatcacaa gactatgcca ttggggtaag ttgtgtttca acggaaagtg ctgtcttaaa 360ctaaatgtgc aatagaaggn gatggtgcca tcctaccgnc ttttcctggt tcctanctgn 420gtgaatacct gctacgtcaa atgcntacca ggttcattct ncctttnact aaaacacaca 480ggtgcaacag acttgaatgc taagtatacc taattggata tgggatttaa ttttctttct 540tacaancatt tgtattgcta acaggccaaa atttcagtta cccttagggt ggttaacaat 600cnaattaaac ctgggaggca tacnttgnct aaatattact gnaaaaaa 648 342 342 DNA Homo sapiens misc_feature (1)...(342) n = A,T,C or G 342ggtacttttt tttttttttt tttttttttt gttttttttt tttttttttt tttttttttt 60tggctntana gggggtanag ggggtgctat agggtaaata cgggccctat ttcaaanatt 120tttaggggaa ttaattctag gacnatgggc atgaaactgn ggtttgctcc acanatttca 180nagcattgac cgtagtatac ccccggtcgt gtancggtga aagtggtttg gtttaaacgt 240ccgggaattg catctgtttt taagcctaat gtggggacag ctnatgagtg caaaacgtct 300tgngatgtaa ttattatacc aatgggggct ttaatcggga at 342 343 484 DNA Homo sapiens misc_feature (1)...(484) n = A,T,C or G 343ggtacgatgc ctagtgatga gtttgctaat acaatgccag tcaggccacc tacggtgaaa 60agaaagatga atcctagggc tcagagcact gcagcagatc atttcatatt gcttccgtgg 120agtgtggcga gtcagctaaa tactttgacg ccggtgggga tagcgatgat tatggtagcg 180gaggtgaaat atgctcgtgt gtctacgtct attcctactg taaatatatg gtgtgctcac 240acgataaacc ctaggaagcc aattgatatc atagctcaga ccatacctat gtatccaaat 300ggttcttttt ttccggagta gtaagttaca atatgggaga ttattccgaa cctggtagga 360taagaatata aacttcaggg tgaccgaaaa atcagaatan gtgttggtat agaatggggt 420cttcttcttc ngcggggtcn aanaaggtgg tggtnccgcg tcctggccng gcnggcgctc 480gaan 484 344 657 DNA Homo sapiens misc_feature (1)...(657) n = A,T,C or G 344cgaggtacgc gggattgttc tggggcttgt cctcctttct gttacggtcc agggcaaggt 60ctttgaaagg tgtgagttgg ccagaactct gaaaagattg ggaatggatg gctacagggg 120aatcagccta gcaaactgga tgtgtttggc caaatgggag agtggttaca acacacgagc 180tacaaactac aatgctggag acagaagcac tgattatggg atatttcaga tcaatagccg 240ctactggtgt aatgatggca aaaccccagg agcagttaat gcctgtcatt tatcctgcag 300tgctttgctg caagataaca tcgctgatgc tgtagcttgt gcaaaaangg ttgtcccgtg 360atccacaagg cattaagagc atgggtggca tggagaaatc gttgtcaaaa cagagatgtc 420cgcagtatgt tcaanggtgt ggagtgtaac tncagaattt tccntcttca ctcatttggc 480tctctacatt aaggagtagg aaataantga aaggtcccct ccattaattt cccttcaaca 540aataattttt tccgaaacng gaccaaatat ggccttcttn tagannataa tgtcntaagg 600ggnatttatt ttaagcnnca canttttaat ttgcaaatna ctatctgggg aaaatac 657 345 662 DNA Homo sapiens misc_feature (1)...(662) n = A,T,C or G 345ggtacgcggg cgactcttag cggtggatca ctcggctcgt gcgtcgatga agaacgcagc 60tagctgcgag aattaatgtg aattgcagga cacattgatc atcgacactt cgaacgcact 120tgcngccccg ggttcctccc ggggctacgc ctgtctgagc gtctcttgca aaaaaaaaat 180aaannanaan acancaagta caatttaatg cntanaaagg cctctctcca taaaactcan 240cnctttacag atgtangaat atataagcnn tgccaaaatt actaatntgc cacatacnna 300gcatcaattc caggtgctag tnagngggaa aaaaanttgg agaattcggc cctcgangag 360ctccanannt taanctncct tactaantnc canggttctt tcaagcatgg aaaaattaat 420ngtgctncat ngatnaangn cttgtcattg ggccttnttt cctngacctg gcccggccgn 480ccgttcnaaa ggctaaatcc agacactgcg gccgttntaa tggttcnnac ttgggccaag 540cttgggnaat catgggcaaa gctgttcctg ggnnaaatnt tatccnctcc aattcncaca 600natacgaanc tgaancttaa gtgtnanntn gggngctaaa agtggcnaan ctcccttnat 660gg 662 346 654 DNA Homo sapiens misc_feature (1)...(654) n = A,T,C or G 346acttcttggc cgcctcacta gcactctccg cctgcttttt aaaggcttca ttggaggcca 60gcagcgtggc ctgctgcgaa atgagagtca ccaggcgtct aagcaggaag gacagcagcg 120aggaaaagcc agcaatgtag agattcctct gggcacggaa aagcttcatg tggaagtgct 180ccatggcccc gggattgttc tggaggttca ccttttccgt cacatcatca tacttccgaa 240tttcgcgcac ggcatcgatg accaacagca caaggatgac aatgagaacc acaaagaagg 300tgttgccata ggacactaac aactccacca gccgggactt gaaaatcttc tgccatcttt 360taggagaaat gaagggaatg cagagaagca acacaacaaa gaccttcgca tagaggaagg 420tggcaactgc agtccactgc agactcatcc tggtgctana agggttccac aggaagatgt 480gaacttgttn cgagtttcca cagtcaacgt gtcccccgta ccttnggccg ngaacacnct 540taaggcgaat tccaccactg cnggccgtct antggatcca actnggncca acttggcgaa 600tatggcaaat tgttctnggg naaatggttc ngtcaattcc ccantacnac cgga 654 347 536 DNA Homo sapiens misc_feature (1)...(536) n = A,T,C or G 347ggtactaatt taaggtaaca attctcgagg taaaataagg cattatagta acacaatttt 60catgcctcag caattaacaa tgattttcgt ttaattctct tccaactcta cagacataat 120tctgctttca ccttcatcac gctttcatat ggttttaaca ggggatacac ctcctcttct 180aagaatctct gcacctgctg ggaggcacga ccagtgaaag aagaaggatc cagtaaatga 240tccaactggg agtgaatggg actgaagtag gcatcaacct ggatacgctc tatgaggnca 300ttgcaccccc ttcctgctta accacagaag ctgcctgctg agaaagcact ctgattttct 360catggcaatc ctggcggcta ccttcacttt gaccatggcc atgatgatgg tctctgtggc 420catgaaangc agctcttgcc gaatgcgccg tcaattactt tggggtacct gcccnggccg 480gccgntcnaa nggcgaattt cagccactgg cngncgtact agnggatcca actcgg 536 348 665 DNA Homo sapiens misc_feature (1)...(665) n = A,T,C or G 348ggtacgcggg gagtcggcgt aggccttagg tgggttcgtg cgccttctac ctcgctgttt 60cggttttcct ggctcctcgg cccttttctc ccctgttgca gctgggagcg gacgaagcgc 120gaagctggga ttttttactg tctcctgaag aatttaacac aaacatggat atcagaccaa 180atcatacaat ttatatcaac aatatgaatg acaaaattaa aaaggaagaa ttgaagagat 240ccctatatgc cctgttttct cagtttggtc atgtggtgga cattgtggct ttaaagacca 300tgaagatgag ggggcaggcc tttgtcatat ttaaggaact gggctcatcc acaaatgcct 360tgagacagct accaggattt ccattttatg gtaaaccaat gccaatacag tatgcaaaaa 420cagattcgga tataatatca aaaatgcgtg gaacttttgc ttaaaaaaaa aaannnnnna 480naaaaaagtc ctgccnggcc gcccgttcaa anggcgaatt naccactggc ggccgttcta 540gnggatccaa ctnggnacca acttggcgta atatggcaaa actggtnccg ngngaaatgg 600tatccgttan aattcccaca cttcaaccgg aacctnaang taaacctggg gcctaagagn 660gacnn 665 349 474 DNA Homo sapiens misc_feature (1)...(474) n = A,T,C or G 349acttcgtcag tttgtaagac atgagtccga aacaactacc agtttggttc ttgaaagatc 60cctgaatcgt gtgcacttac ttgggcgagt gggtcaggac cctgtcttga gacaggtgga 120aggaaaaaat ccagtcacaa tattttctct agcaactaat gagatgtggc gatcagggga 180tagtgaagtt taccaactgg gtgatgtcag tcaaaagaca acatggcaca gaatatcagt 240attccggcca ggcctnagag acgtggcata tcaatatgtg aaaaaggggt ctcgaattta 300tttggaaggg aaaatagact atggtgaata catggataaa aataatgtga ggcgacaagc 360ncaaccatca tagcttgatn atattatatt tctgagtgcc agaccaaaga gaaggagtnt 420aaanggatga tcntcttttg ggcatcattt tgggaccttn ggccgggaac accc 474 350 452 DNA Homo sapiens misc_feature (1)...(452) n = A,T,C or G 350acgcggggac cgtggagagc agagcgcggc ggctggaagc tgctaagtca gagccgcgat 60gttccggatt gagggcctcg cgccgaagct ggacccggag gagatgaaac ggaagatgcg 120cgaggatgtg atctcctcca tacggaactt tctcatctac gtggccctcc tgcgagtcac 180tccatttatc ttaaagaaat tggacagcat atgaagacag gacatcacat atgaatgcac 240gatatgaaga gcctggttac agtttcgact cctctctgca agtgaatagg cccagaaagg 300tgtaagagac tctttgaatg gacataaaat tctgcttgtt aagaacaagt ttggctctgg 360taactgacct tcaaagctaa aatataaaac tatttgggaa agtatgaaac gatgtcttcg 420tgatctggtg taccttggnc gngaccacgc tt 452 351 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 351ggtacgcggg aataattcca tagtcaagag catcacagtc tctgcatctg gaacttctcc 60tggtctctca gctggggcca ctgtcggcat catgattgga gtgctggttg gggttgctct 120gatatagcag ccctggtgta gtttcttcat ttcaggaaga ctgacagttg ttttgcttct 180tccttaaagc atttgcaaca gctacagtct aaaattgctt ctttaccaag gatatttaca 240gaaaagactc tgaccagaga tcgagaccat cctagccaac atcgtgaaac cccatctcta 300ctaaaaatac aaaaatgagc tgggcttggt ggcgcgcacc tgtagtccca gttactnggg 360aggctgaggc aggagaatng cttgaacccg gnaggtggag attgcagtga gccagatcgn 420acnactgnac tcagtctggc aantgagnag gcttccatct nanaangaan aganangang 480actntnacct ggacctgccn ggccggtcgt ttgngcaggt cnggagattt attcccttng 540ggtggggngc nntaattggn tgntgggccn attcangttt tgggaatttc nncttggnnn 600naaaanggga aatttt 616 352 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,C or G 352ggtacggcac ttggcgtaaa gccgcttccc tcaagagtaa ctacaatctt cccatgcaca 60agatgattaa tacagatctt agcagaatct tgaaaagccc agagatccaa agagcccttc 120gagcaccacg caagaagatc catcgcagag tcctaaagaa gaacccactg aaaaacttga 180gaatcatgtt gaagctaaac ccatatgcaa agaccatgcg ccggaacacc attcttcgcc 240aggccaggaa tcacaagctc cgggtggata aggcagctgc tgcagcagcg gcactacaag 300ccaaatcaga tgagaaggcg gcggttgcag gcaagaagcc tgtggtaggt aagaaaggaa 360agaaggctgc tgttggtgtt aagaagcaga agaagcctct ggtgggaaaa aaggcagcag 420ctaccaagaa aaccagcccc tgaaaagaac ctgcagagaa gaaacctact acngaggaga 480agaagcctgc tgcataactc ttaaatttga atatttcntt aagggcnaat nttttggcag 540gttctttgga taagacntnt tttcngngtg ggaaaataan tnnnntattn nnggctntcc 600tgg 603 353 604 DNA Homo sapiens misc_feature (1)...(604) n = A,T,C or G 353ggtaccgact gtttttgaca actatgcagt cacagttatg attggtggag aaccatatac 60tcttggactt tttgatactg cagggcaaga ggattatgac agattacgac cgctgagtta 120tccacaaaca gatgtatttc tagtctgttt ttcagtggtc tctccatctt catttgaaaa 180cgtgaaagaa aagtgggtgc ctgagataac tcaccactgt ccaaagactc ctttcttgct 240tgttgggact caaattgatc tcagagatga cccctctact attgagaaac ttgccaagaa 300caaacagaag cctatcactc cagagactgc tgaaaagctg gcccgtgacc tgaaggctgt 360caagtatgtg gagtgttctg cacttacaca gaaaggccta aagaatgtat ttgacgaagc 420aatattggct gccctggacc tncagaccga agaagacccc aagtgtgtgc tgctatgaac 480atctttcaga gcctttcttg nacagctgga ttggcatctt cttaaagcca tgnttaaatt 540caacttanga ttaaaattaa aattcgtttt gcannatggc caatgcctgg actaacccan 600ggcn 604 354 631 DNA Homo sapiens misc_feature (1)...(631) n = A,T,C or G 354ggtacttttt tttttttttt tttttttttt tttgggacgg agtcatgctc tgtcgcccag 60gctggagtgc agtggcatga tctcggctca ctgcaagctc cgcctcccgg gctcatgcca 120ttctcctgcc tcagcctccc gagtagctga gattataggc acctaccacc acgcccggct 180aatttttgta tttttagtag agacggggtt tcaccatgtt gaccaggctg gtctcgaact 240cctgacctta ggtgatccac tcgccttcat ctcccaaagt gctgggatta caggcgtgag 300ccaccgtgcc tggccacgcc caactaattt ttgnattttt agtaagagac agggtttcac 360catgttggcc aaggctgctc tttgaactcc tgacctcatg taatcgacct gcctttggcc 420ttccaaaagt gctgggatta ccaggtgtga gcccacaagc cccggnacct ggccnggcng 480gccgtttaaa agggcgaatt cagcacaatg gnnggccgta ctaaggggat ncnanctttg 540nanccaactt tgggggaaat atggggcana actggttcct ngngnaaatg gtaaccgtta 600caaattcccn caaanttttg nnccgggagg n 631 355 626 DNA Homo sapiens misc_feature (1)...(626) n = A,T,C or G 355ggtacgatgc ctagtgatga gtttgctaat acaatgccag tcaggccacc tacggtgaaa 60agaaagatga atcctagggc tcagagcact gcagcagatc atttcatatt gcttccgtgg 120agtgtggcga gtcagctaaa tactttgacg ccggtgggga tagcgatgat tatggtagcg 180gaggtgaaat atgctcgtgt gtctacgtct attcctactg taaatatatg gtgtgctcac 240acgataaacc ctaggaagcc aattgatatc atagctcaga ccatacctat gtatccaaat 300ggttcttttt ttccggagta gtaagttaca atatgggaga ttattccgaa gcctggtagg 360ataagaatat aaacttcagg gtgaccngaa aaatcagaat aggtgtttgg tttagaatgg 420ngtcttctnc ttcngctggg gtnnaagaan gtnggggttc nngcgtnctn gntcgggcgg 480ntggttttaa nggccnaaat tcnngnataa ttggcggcng ttactaagng gnatctanct 540tggtnccaaa nttggngnta atcatggtnc tagctngtnc tcngtgntaa attggntncc 600tgttaaattn tntnnaatnt tntggc 626 356 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 356actttttttt tttttttttt tttttttcta gtttcagtta tttattgatt taatcattgt 60aatctccaat agagattaca atagagatct ccaacatgat ttcatgcatt tagaggagaa 120atatttcctg gttaagtgga aaattgtgcg gatgtggctt ctggaanacc ttcattctaa 180agcagcgtta tagtgaaaca tttcatttan aaatctggac gttccttctt cagcttgctg 240taatccacat tcactgagta naacttgtat tgatcattgg gacccagttt gttccagggc 300tctgggttat ttctgtccca acaaacatct ggattgaaca atgccagacg caagagatac 360agtgttgctc cagtagctcc agttccaata aatacnaaga gggggatcaa gctcggatgc 420ttcttggcct gaccgatgat ctggccggaa ncatgtttgc cggcaaaagg ctccnacttg 480ggaaagggga naacccgcct aaccnccagg gcctaagctt aaaatttttg gccccgggta 540ccttggccgg gaccccctaa gggngnaatt ccnncccctt ggggggccgt ttaangggan 600ccaacttggn ccaaatt 617 357 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 357ggtacttttt tttttttttt tttttttttt ttttaggcaa agaactttat taatctttgt 60ttcaaacttg attcccaggc ttcttcggct taattagctg caaagaatga attgngtata 120agcaaaaact gaaaagagct gcagtgtcca aggggcttgg gcttaaaaat attagagatc 180tagattttat cagatccata aacaaaaatt tcttaaaaag cagtcataat ataaaatagc 240agctcccagt aacttcttca ggntttatct tcagaagttg actcaattca gtttgcctca 300ttcttggaag cctcatcaaa attctccaca agatctggaa cttcatcatc atcatcctct 360ccagtaacaa gtggngcttt tccatcccca gantggttgg gcanaacttt ngnccagctc 420cttaacttag cagactattc ggacccaagc tnggttnaaa aanctgggaa cnatttntgn 480naactggttt ggttnaacan ggcntgnaag ggggaaaggn gtnccctgcc caaaaaaccn 540ggacctttag ggtgnnaaag gggacctggc cctgggttgg aaccaantcn ccttttnana 600ccnnanaatn g 611 358 619 DNA Homo sapiens misc_feature (1)...(619) n = A,T,C or G 358ggtacttttt tttttttttt tttttttttt ttgagatgga gtctcgctct gtcgcccagg 60ctggagtgca gtggcgcaat ctctgctcac tgcaacctcc gcctcctggg ttcaagcaat 120tctcctgtct cagcctccca aatagctggg attacgggca tgtgtcacga cgctcggcta 180atttttgtat ttttagtcga gacgaggttc caccatgttg gctaggctgg tctcaaactc 240ctgacctcag gtgatccgcc tgcctcggcc tcccaaagtg ttaggattac gggtgtgagc 300cactgcgccc agcaagcaac ctagatttta aaacaacatg agataaataa gcctaattgg 360atttaactac atctaacatt tttactaata gttgnaatac tggtagaatt tggaaactat 420tatatatatt atgcngaaaa gtaaataatt ctggtaaaat canttanggn ccntgaattt 480nagcataggg gaaaaaaaga tgccntttta aatccaataa gtaaaaaccn tttaaccctn 540tntttaaatt ggaanttccc cccaatttnt tattaatttc aacttntttt gaaaactcat 600ntttccnaaa antnggggg 619 359 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,C or G 359ggactttttt tttttttttt tttttttttt ttttttggag gaaaacccgg taatgatgtc 60ggggttgagg gataggagga gaatggggga taggtgtatg aacatgaggg tgctttctcg 120tgtgaatgag ggttttatgt tgttaatgtg gtgggtgagt gagccccatt gtgttgtggt 180aaatatgtag agggagtata gggctgtgac tagtatgttg agtcctgtaa gtagganagt 240gatatttgat caggagaacg tggttactag cacagagagt tctcccagta ggttaatagt 300ggggggtaag gcgaggttag cgaggcttgt tanaagtcat caaaaagcta ttagtgggag 360tagagtttga agtccttgag agaggattat gatgccactg ngaatgcntt cctaatttga 420gtttgctagg cagaatagtn atgaggatgt aaacccctng gccaattatt aaaaatgact 480gcncccgtga aacttnaggg ggtttggatt aaaaangctt gtacttccaa nggctntntg 540gcctnattta aaaaatttcc ctnnncnaat ttagggcttn ttnncnnaag ccnanagggn 600ccccnancct ttcccggggg ggcn 624 360 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 360acgcggggag gcggaggctt gggtgcgttc aagattcaac ttcacccgta acccaccgcc 60atggccgagg aaggcattgc tgctggaggt gtaatggacg ttaatactgc tttacaagag 120gttctgaaga ctgtcctcat ccacgatggc ctagcacgtg gaattcgcga agctgccaaa 180gccttagaca agcgccaagc ccatctttgt gtgcttgcat ccaactgtga tgagcctatg 240tatgtcaagt tggtggaggc cctttgtgct gaacaccaaa tcaacctaat taaggttgat 300gacaacaaga aactaggaga atgggtaggc ctttgtaaaa ttgacagaga ggggaaaccc 360cgtaaagtgg ttggttgcag ttgtgtagta attaangact atggcaagga gtctcagcca 420aggatgtcat tgaagagtat ttcaaatgcc agaaatgaag aaattaaatc nttggcttac 480ttaaaaaaaa annnnnnnnn aaaaaaaagg tccttgggcg gnacaccctt aaggggnaat 540tcnnnnccct gggggccntt ataangggnn ccnacttggg ccaaattggg naaananggg 600naaanttttt n 611 361 404 DNA Homo sapiens misc_feature (1)...(404) n = A,T,C or G 361acatatttta atagaaagat acaacctttt tattttcact ccttttattt ctgctgcttg 60gcacattttt gagttttccc acattatttg tctccatgat accactcaag cagtgtgctg 120gacctaaaat actgacttta gttagtatcc ttggattttt agattcccag tgtctaattc 180cctgttataa tttgcgcaaa caaaacaaaa tgttatgata atctttctcc actgttctaa 240tatatattgt atttttattt gatagcttgg gatttaaaac atctctgttg aaggcttttg 300atccttttga gaaataaaga tctgaaagaa atggcataat cttaaaactt gataaaaaaa 360aaanannnnn nnnaaaaaaa aaagtacctn ggccgngacc acgc 404 362 322 DNA Homo sapiens misc_feature (1)...(322) n = A,T,C or G 362ggtacttttt tttttttttt tttttttttt ttttttggag ttgtaggcaa atgtttaatt 60aattctgctc atatgcacat ctgaaagcat gagacacact ccacagacag cacgcactgg 120ggctggtggg gcanatgggc actcgccgat taggtattaa tgtcaataat acgtgcataa 180agtgctgata aaataactta agtgttacaa aaagagacag tccacggtgg ctgcaggcac 240atgcaggcgg gactgggtca gacactccag ggctgcacat gttccagctg gcctgagtcc 300gacacgtcat agctggcctt gt 322 363 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 363cgaggtacgc gggctaagca agggaaaaat aacagtttct ctgagccaga gaagacttga 60tcacagttct ccaagcatcg tgatagcaat gcttaacccc aggaagattt caaggcaggg 120agaagaacat ttcaaataag attcttgtta acccatttat gcctagtgtt ccattattgg 180aatgctaagc ttgtgggagt catttacatc ctactgctca aagtcattgc caaggtctga 240tttttcacac aaaaaattgc aacccccagc ataaatgttt agctactgtc atcagttagc 300aaattcatcc acacaaacac aattagagtt tggttttttt ttaagctttt caaaacttac 360taaactggca caattttata tgtatgctat ttggtgnatt tatgcttaag agcnaaaaag 420tttgatggga ttttaaattc angccaagcc tacacgctga gacaatccct acaaccatgg 480nagtaactaa ngaaccttta tctaagnttt taagttttaa anggagngct taatggttca 540ngtctangtt ggaatttcct tcanaaattt cntcttttaa aaaattttcc caaaatnggt 600ccttaaaaaa ctcann 616 364 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 364cgaggtacgc ggggcttctc gcctaacgcc gccaacatgg tgttcaggcg cttcgtggag 60gttggccggg tggcctatgt ctcctttgga cctcatgccg gaaaattggt cgcgattgta 120gatgttattg atcagaacag ggctttggtc gatggacctt acactcaagt gaggagacag 180gccatgcctt tcaaatgcat gcagctcact gatttcatcc tcaagtttcc gcacagtgcc 240caccagaagt atgtccgaca agcctggcag aaggcagaca tcaatacaaa atgggcagcc 300acacgatggg ccaagaagat tgaagccaga gaaaggaaag ccaagatgac agattttgat 360cgttttaaag ttatgaaggc aaagaaaatg aggaacagaa taatcaagaa tgaaagttaa 420agaaacttca aaaggcagct nttctgaaag cttnttccca aaaaagcacc tgggtacctg 480gccgggccgg ccgtttaaaa gggcnaattc caccactggc ggccgtctan ngggatccaa 540cttnggacca acttggngga atatggcnaa attgttcctg gggnaaatgt ttncgttcaa 600attncncaaa ttacggcc 618 365 601 DNA Homo sapiens misc_feature (1)...(601) n = A,T,C or G 365acgtcctgga ggactctatt gtggacccac agaatcagac catgactacc ttcacctgga 60acatcaacca cgcccggctg atggtggtgg aggaacgatg tgtttactgt gtgaactctg 120acaacagtgg ctggactgaa atccgccggg aagcctgggt ctcctctagc ttatttggtg 180tctccagagc tgtccaggaa tttggtcttg cccggttcaa aagcaacgtg accaagacta 240tgaagggttt tgaatatatc ttggctaagc tgcaaggcga ggccccttcc aaaacacttg 300ttgagacagc caaggaagcc aaggagaagg caaaggagac ggcactggca gctacagaga 360agccaaggac ctcgccagca aggcggccac caagaacagc agcagcagca acagtttgtg 420taaccagnct accaacaaca nagnacccca nacaggtagg cttacccctt tggcctcctt 480taatggacct tggccgggaa cacccttang gcgaattcag ncactggggg ccgtactang 540ggatccnctt ggaccaactt ggggaaacag ggcaaaattg ttcttgggga aattntatcc 600n 601 366 321 DNA Homo sapiens 366actttttttt tttttttttt tttttttgag atggagtctc actctgtcgc ccaggctgga 60atgcagtggt gcaatctcag ctcactgcaa cttccacctc ccaggttcaa gtgattctcc 120tgcctcagcc tcccacatat ctgggactac aggtgcacac caccatgccc agctaatttc 180tttgtatttt ttagtagaga cggggtttca tcttattggg caggctggtc tcgaactcct 240aaccttgtga tctgcccacc tcggccttcc aaagtgctgg gattacaggc gtgagccacc 300gtgctcggcc acccgcgtac c 321 367 264 DNA Homo sapiens 367actgatcatg gagttaatca acaatgtcgc caaagcccat ggtggttact ctgtgtttgc 60tggtgttggt gagaggaccc gtgaaggcaa tgatttatac catgaaatga ttgaatctgg 120tgttatcaac ttaaaagatg ccacctctaa ggtagcgctg gtatatggtc aaatgaatga 180accacctggt gctcgtgccc gggtagctct gactgggctg actgtggctg aatacttcag 240agaccaagaa ggtcaagatg tacc 264 368 488 DNA Homo sapiens misc_feature (1)...(488) n = A,T,C or G 368ggtacagatg cacaggaggc catagggttt aggcanaggg gagcacaaan gttgaagatg 60aggcgctgcc atcaatgctg ggacttcagg cnaagggcag gaactgagga agccacaagg 120gaggacattt tctgcagttg ctgaancagt ancaactagg tcctgagaaa gccctntctc 180gtggaagaat aacagccagg cnggaaagct tttcatcctg caaagctggg gaagaagatt 240cttccttaaa ttgtcatctg cacttcagct cangaatcct gttggctgaa gtccagagtg 300tccntttctg attcctgaag tanatnaaca gcccngnccc aangaagagn aggnntagta 360caaagccnnc tncgcgtacc tgtncgggcg gnngttcgna aggntcaaat tccagcacaa 420ttgnctgccg ttantagttg gattctnact ttngtactta ncttggcgta ntttatggtn 480ataanttg 488 369 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,C or G 369acgggggttt cactacttct cccccggact ccttggtagt ctgttagtgg gagatccttg 60ttgccgtccc ttcgcctcct tcaccgccgc agaccccttc aagttctagt catgcgtgag 120tgcatctcca tccacgttgg ccaggctggt gtccagattg gcaatgcctg ctgggagctc 180tactgcctgg aacacggcat ccagcccgat ggccagatgc caagtgacaa gaccattggg 240ggaggagatg attccttcaa caccttcttc agtgaaacgg gtgctggcaa gcatgtgccc 300cgggcagtgt ttgtagactt ggaacccaca gtcattgatg aagttcgcac tggcacttac 360cggcagctct tcaccctgag caactcatca caggcnagga aaaatgctgc aataactatc 420ccgaaggcac tacaccattg gcaaggagaa taattgacct gtgttggacc gaattcgcaa 480gctggctgac catgcaccgg cttaagggtt nttggttttc ccaacttttg gggggggaac 540tgggtttngg gtaaccctnn tggtnatngg aacgntttta antggatttt gggaanaaan 600cc 602 370 257 DNA Homo sapiens misc_feature (1)...(257) n = A,T,C or G 370actttttttt tttttttttt tttagttttt ttttattttt tacaaatata ctggagaatc 60atgcaatgct gccagcattg gatgcaatcc ggggccacaa gtctgcacac tcctttgcta 120ctggtcctgt aatggcagaa cctttcatct cgcctttatt gntcactatg actcctgcat 180tatcttcaaa ataaagaaac acgccatctt ttctacggta tgactttcgt tgtcgaatga 240ccactgctgg atgtacc 257 371 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 371actttttttt tttttttttt tttttttgct atttagtttt tatttcataa tcataaactt 60aactctgcaa tccagctagg catgggaggg aacaaggaaa acatggaacc caaagggaac 120tgcagcgaga gcacaaagat tctaggatac tgcgagcaaa tggggtggag gggtgctctc 180ctgagctaca gaaggaatga tctggtggtt aagataaaac acaagtcaaa cttattcgag 240ttgtccacag tcagcaatgg tgatcttctt gctggtcttg ccattcctgg acccaaagcg 300ctccatggcc tccacaatat tcatgccttc tttcactttg ccaaacacca catgcttgcc 360atccaaccac tcagtcttgg caagtgcaga tgaaaaactg ggaaccantt ggggttgggt 420ccacatttgc catggacaag aatgccagga acccgtatgc tttaaggatg aagtctcatc 480ttcaaaattc ttccccataa atggacttgc caccagngcc attatggcgt gtgaagtccc 540cancctggcc cataaaccct ggaaaaatnt tggnaaaccg gaaccctttt aaccaatcct 600ttttttc 607 372 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 372acgaatgtgg gaattactca ggagcagcag aatatcttta tttttttaga gtgctggttc 60cagcaacaga tagaaatgct ttaagttcac tctggggaaa gctggcctct gaaatcttaa 120tgcagaattg ggatgcagcc atggaagacc ttacacggtt aaaagagacc atagataata 180attctgtgag ttctccactt cagtctcttc agcagagaac atggctcatt cactggtctc 240tgtttgtttt cttcaatcac cccaaaggtc gcgataatat tattgacctc ttcctttatc 300agccacaata tcttaatgca attcagacaa tgtgtccaca cattcttcgc tatttgacta 360cagcagtcat aacaaacaag gatgttcgaa aacgtcggca ggttctaaaa agatctaggt 420taaaggttat tcaacangga gtcttacnca tntaagaccc cattacngga atttggtgaa 480tggttatatg taactttgac tttaangggc tcaaaaaaag ctnaggggat gtgaatcaag 540cttgngaagg ctttttttgg gggctngntt nngggtttnt tgnaaagncc ngttttnntt 600ttggaat 607 373 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 373acttttaatg tttgctgttc aaacgaaaat agattggatc ttggttaagt tcacttggtt 60tggccaggca cagtggctca cgcctgcagt cccagcactt ggggaggtgg aggcgggccg 120atcacctgag gtcaagagtt tgagaccagc ctggctaacg cggtgaaacc ccatttctac 180taaaaataca aaaaattagc tgggcgtggt ggtgcgcgct tgtaatccca gctactcggg 240aggctgaggc aggagaatcg cttgagccag agaggcaaag gttgcaataa gccaagatag 300cgccattgta ttccagcttg gacaacaaga gcgaaactct gtctaaaaaa aaaaaaaaaa 360cacacacaca acacaatatt ttcacgcctg taaacctagc acattgggaa gccaaggtgg 420gaggattgct tgaggccagg agttcaaggc ttgcantgag ctatgaatgn acactgnacc 480tttggncgng aacacnctta nggccaaatt ccngcacact tgggggccgg tactaanggg 540atcccanctt tggnnccaaa nttggngnaa acatgggcaa aattggtncc tggngaaaat 600ggttccgttc caaatccc 618 374 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 374acccagctgc tgcccacatt tctggtccag agtcccgaac cccgagcact gggatgcctg 60gctactccga gcgttatcca gactagcgag tgggaggcag atgtaaaatc tggaacgcag 120attttagttt gttggaagga gaaatgtaac atagtgaacc acgcatctct ggagggtgta 180aagcagagac agccaagagc caaggcactg atgtttgaac tggaaacttc aaaacgttta 240ataagagtct tcaggatggg tttgaactag acaagctaga aatttcttta gaacaccagc 300tctagcatgc atctcccact tttggctttc ctggagagga gcttgaagag gtggttctgc 360agacagccac agtgatactc aggaaacnca gaggaatgga tttgactttt ctgctaggaa 420tctttggtat aagttctcct tgagttgtaa gangcatgga aatatacatg aaactgaana 480acctgcaagg aanggaaatg ggaacntttc atctgagtgn aaactaacca agtnggcaat 540ttngacttga aacccttgaa accttcnagt ccaantcctg gtttggggga taaangaacc 600ggncn 605 375 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,C or G 375acggatgcta cttgtccaat gatggtaaaa gggtagctta ctggttgtcc tccgattcag 60gttagaatga ggaggtctgc ggctaggagt caataaagtg attggcttag tgggcgaaat 120attatgcttt gttgtttgga tatatggagg atggggatta ttgctaggat gaggatggat 180agtaataggg caaggacgcc tcctagcttg ttagggacgg atcggagaat tgtgtaggcg 240aataggaaat atcattcggg cttgatgtgg ggaggggtgt ttaaggggtt ggctagggta 300taattgtctg ggtcgcctag gaggtctggt gagaatagtg ttaatgtcat taaggagaga 360atgaanagaa gtaagccgag ggcgtctttg attgtgtagt aagggtggaa ggtgatttta 420tcggaatggg aagtgattnc taaggggntg tttgancccc gtttgtgcca gaatangaag 480tggaatgctt cttanggctt caataaatga anggcanaat gaattgaaag gtaaanaaac 540cntnaagggt ggacttgtta ctgataaccn tcctaaaatc attgccccgn aacttggccg 600gg 602 376 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 376acgcgggatc gaagaattca caaaaaacaa tagcctcatc atccccacca tcatagccac 60catcaccctc cttaacctct acttctacct acgcctaatc tactccacct caatcacact 120actccccata tctaacaacg taaaaataaa atgacagttt gaacatacaa aacccacccc 180attcctcccc acactcatcg cccttaccac gctactccta cctatctccc cttttatact 240aataatctta taaaaaaaaa aaaaaaaaaa aaaaaaaaaa ncaaaaaaaa aaaaanaaaa 300aaaaaaaang tncngccatt tttngtttcn ggtaaacngg aatataangn gaaagaacaa 360acnttggaac atacttaatg gatttttata gaactttgna aaccaaagga gattcatgtt 420ttanaagtct ggcctttttt atatcttgga agaaaattat gtntggaggc tntaaataaa 480tcccattatt ttctcaggga atctgggtag gaattgccgg catgggaant tttnnggggc 540cggatnggaa agtttggcct aanaaatngc nctttntnaa naattttgga attttgggaa 600gcccnaagca n 611 377 367 DNA Homo sapiens misc_feature (1)...(367) n = A,T,C or G 377acgcgggccg tttggcatct ctgccctcat cgtgggtttc gactttgatg tcactcctag 60gctctatcag actgacccct cgggcacata ccatgcctgg aaggccaatg ccataggccg 120gggtgccaag tcagtgcgtg agttcctgga gaagaactat actgacgaag ccattgaaac 180agatgatctg accattaagc tggtgatcaa ggcactcctg gaagtggttc agtcaggtgg 240caaaaacatt gaacttgctg tcatgaggcg agatcaatcc ctcaagattt taaatcctga 300agaaattgag aagtatgttg caaaaaaaaa aananaaatn aaanaagtac ctcggccgng 360accacgc 367 378 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 378ggtacctgga tcctgctcct ctgggttgaa acccgggcgc cgccaagatg ccggcttacc 60actcttctct catggatcct gataccaaac tcatcggaaa catggcactg ttgcctatca 120gaagtcaatt caaaggacct gcccccagag agacaaaaga tacagatatt gtggatgaag 180ccatctatta cttcaaggcc aatgtcttct tcaaaaacta tgaaattaag aatgaagctg 240ataggacctt gatatatata actctctaca tttctgaatg tctgaagaaa ctgcaaaagt 300gcaattccaa aagccaaggt gagaaagaaa tgtatacgct gggaatcact aattttccat 360tcctggagag cctggttttc cacttaacgc aatttatgcc aaacctgcaa acaaacaggg 420aagatgaagt gatgagagcc tatttacaac agcttaaggg caagaaactg gactggaact 480ttgtgaagaa gttttcgacc cttagaatgg ttaaaccnac agtgggggga cttgcttttg 540gaaaanaccg tttattgacn anagtttttt tggactggan atgaaaggng cccnggttng 600ccccggtttn n 611 379 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,C or G 379acagctggtt ggacctattc atgcatcttc accagcagct ggagcatctc cacccttggt 60atttctggtg taaattactt gagctctgtg ctttgaaacc agtttgataa gtcctttact 120aaggagctcc tgaagggctg ccctggccag ggagcctcga atcttcagtc tctcagagac 180cacagctggg gttataagtt tatagttggg aacttcctta cagagtttat cataggtagc 240tttgtcaaac aagactaagt tattgagctt gtcccgaact ttgcctttgg accacttctt 300ctttttggcc ttgcccccgg atttgttcac tgggtctttg nctttcttgg ccgactttcc 360agcgtccttc ttcttcttgt cgtccttagg cggcattgcc aagctcggag aatagcanca 420gacacngnaa cctngtcaag atgtcngaca aaaagccccg ggtaccttgg gcgngaacac 480gcttaaggcg aattccacac actggcggcc gtactanggg gatccagctt nggaccaact 540tggnggaaac atggcnaact gnttcctngn ggaaaatgtn atccgttaaa attnccccaa 600at 602 380 598 DNA Homo sapiens misc_feature (1)...(598) n = A,T,C or G 380ggtacngcgg ggggtgcctg gctccgtttc ctgcttttgg ttcttacagt agtcggcgta 60ggccttagat tttttactgt ctcctgaaga atttaacaca aacatggata tcagaccaaa 120tcatacaatt tatatcaaca atatgaatga caaaattaaa aaggaagaat tgaagagatc 180cctatatgcc ctgttttctc agtttggtca tgtggtggac attgtggctt taaagaccat 240gaagatgagg gggcaggcct ttgtcatatt taaggaactg ggctcatcca caaatgcctt 300gagacagcta caaggatttc cattttatgg taaaccaatg cgaatcagta tgcaaaacag 360attccggata taatatcaaa aatgcgtgga acttttgttg ccaagaaaag aanaaagaaa 420agaaaaagnc caaacttggg aacaactgna caaccncaac caaaaanctg ggcnngggac 480tccaaatcac ttatacccag ggaattcacc ccnaatctta ggtcctgata ccttcaacta 540tatttaatcc ttaaaactta nccgaagagc taatngatga tgtntcctgc cggtaacn 598 381 631 DNA Homo sapiens misc_feature (1)...(631) n = A,T,C or G 381ggtacgcggg gagagtgtgg tcaggcggct cggactgagc aggactttcc ttatcccagt 60tgattgtgca gaatacactg cctgtcgctt gtcttctatt caccatggct tcttctgata 120tccaggtgaa agaactggag aagcgtgcct caggccaggc ttttgagctg attctcagcc 180ctcggtcaaa agaatctgtt ccagaattcc ccctttcccc tccaaagaag aaggatcttt 240ccctggagga aattcagaag aaattagaag ctgcagaaga aagacgcaag tcccatgaag 300ctgaggtctt gaagcagctg gctgagaaac gagagcacga gaaagaagtg ctttagaagg 360caatagaaga agaaccacaa cttcgtaaaa atggcngaan aagaaactga ccnccaaaat 420gggagcttat taaagagaan ccagangnnc caatngnttg gccaactggg accgtttgca 480anaagaaggg ttagccccnt tgaanaaatg ccggaagaac caaagaattc caagaccctt 540gntgcnaaac ttgaacttgc ctaattggtc ttgagaactg cttttttccc atcccttcta 600aaatccaaaa atgnacctgc ccgggggccg t 631 382 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 382acattcccag atttttaagc ctccctcata aacacctgta atcagatcag agtgagaaga 60aaagcttttt gaaactatgt tttctccagg gaagttctct ttcaacaaga tggttttcac 120tactgataac ttaacatgct ggaaacctgg taatgtttct atgactttat tttctaacat 180cttctttaaa tctttaggca tagcatgctc tttggcagct ctcaaggagg gctgtttcca 240tgtggctcca agttccttga actgctggct gcactgagtg gactgtctgt gtcttgagag 300ggagctgcat tttcattgac ttatggtccc acaagtgacc ctgaggcaan gtcnaattgg 360tctncanaac atttttggcc ctctcttctc ctttttgact tttctgagac tgacagttct 420tttganggaa tccagggnna angcttccnt ctctaatggg ggntaaattc attttccaaa 480anggncggtt tttgggaaaa tnaaanttga aanggcatcc nttttattaa tgccccnanc 540ttttaanttc ngattntnaa cttnctgnta gaatttgtgg atccnccaaa ttggcttaat 600attcaaatag ctt 613 383 628 DNA Homo sapiens misc_feature (1)...(628) n = A,T,C or G 383ggtactttga ccctggaaag gtatgggtct gcttaaaaga aagaagaaac atacacgtaa 60tcaaataaag cttaacatta tgcagggctt ataatcattt tcagcaacgg actgcaagct 120gcactgtgaa gaaaatgcat agcagaggag aaagctgggg atctgaggaa ataggtaagg 180aaaacagtgt caacacacag tggaagaagt gatgaagaca tctattccgg agctcacgtg 240ccatgccctg ctagcgttcc ttaacaagcc acctgctcca gaaggccaca gcctgaccct 300cccaagtgga atataaatgc ccaagtgcca catgaagcca ccttctncac tacctaaaaa 360ggttgtctgg gactgagctc agaacacaca cctttctggg ctaccaaacc tttaagtgga 420aagaattttt tnctaaatat ctanttttna tacccacttt aacgccactt ttatattgaa 480attgggcttc taattagncc ctttcctcaa ttccttagga nggaactcat aatgggagcc 540aaccaaccag ggattctacc cccaatngac tgnnctttaa angtattatt aattttgang 600ggcaaaggtg tgaatggttt acaatacc 628 384 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 384acaggtaagc cctggctgcc tccacccact cccagggaga ccaaaagcct tcatacatct 60caagttgggg gacaaaaaaa gggggaaggg ggggcacgaa ggctcatcat tcaaaataaa 120acaaaataaa aaagtattaa agcgaagatt aaaaaaattt tgcattacat aatttacacg 180aaagcaatgc tatcacctnc cctgtgtgga cttgggagag gactgggcca ttctccttag 240agagaagtgg ggnggctttt angatggcaa gggacttcct gtaacaatgc atctcatatt 300ttggaatgac tattaaaaaa acaacaatgt gcaatcnaaa gtctcggccc atttgcggaa 360ctttgggggg atgcttgctt cnaccgantt ggtgncaacc tttnnccggt tccanttttt 420naaattctta gtnnaagcnn aaaaanntag aatancncna nancataact tannaancca 480tttaanaggt ccctcggccg gaacnnnctt aanggtnaat cccantnnnt ggcgggcgtt 540actncnggat ccanccttgg nnccaaantn gnggaattca tggcnnaacc gntcctgggn 600gaantngttn ccttnaaanc 620 385 535 DNA Homo sapiens misc_feature (1)...(535) n = A,T,C or G 385ggtacttttt tttttttttt tttttttggt atttagtttt tatttcataa tcataaactt 60aactctgcaa tccagctagg catgggaggg aacaaggaaa acatggaacc caaagggaac 120tgcagcgaga gcacaaagat tctaggatac tgcgagcaaa tggggtggag gggtgctctc 180ctgagctaca gaaggaatga tctggtggtt aagataaaac acaagtcaaa cttattcgag 240ttgtccacag tcagcaatgg tgatcttctt gctggtcttg ccattcctgg acccaaagcg 300ctccatggcc tcacaatatt catgccttct ttcactttgc caaacaccac atgcttgcca 360tccaaccact cagtcttggc agtgcagatg aaaaactggg aancntttgg ggtngggncn 420acatttgcct tggccaaaat gccnggaacc ggccccgtac cttgnccngg ccggccggtt 480caaaagggcg aattccacac acttggcggg ccgtactang gggatccaac ttcgg 535 386 642 DNA Homo sapiens misc_feature (1)...(642) n = A,T,C or G 386acagcattgg cagtggtgcg tcagaggtgg cagaactatt tcacactaac cagttgaaga 60ctacacaaga ttaataccat ccagcatcag gatatagctg tggattttac aaaccattct 120tatttctaac ttcaggagtt gatgtttttc ccagtccatc ttaaaatatt actgctttaa 180tcacagatca ggtaaaaagg acaacatgca caacctccac ctagaatcct gttgtagcct 240agacagtgaa atgatatgac atcagaagac tttaaaattg cagctccttt tggatccccc 300aaagtgtatc tgcactcttc ttcaaacggg ccctctttcc tcaagaagtc agaagtcacc 360ttcacaangn ctgagaattc cattctgnnc ccaaantgca agggacactn aaggaagaca 420tcattctttt attccgtnaa agacccttaa ttcatgggng gaaactgggt gcacccgcct 480nagaatcttt attanactct ttgnccaatt tggttacaga agagntncan tanccccang 540aannggtagc ctttggagtt tgantcaccc tcataagcac ccttaaacca cctgnttggg 600gaaccttctt tcactggtcc ctaactttat tangccctaa ag 642 387 256 DNA Homo sapiens 387ggaccttttt tttttttttt tttttttttt tgaaaagaaa ggccttacat atttattact 60gaatccagcc aaccaacgtg ttcataacag attcagagag gaaaacacgt cgaaatctcc 120agatagtggt gacattttca gcttgatatg gtaacatgat cgtgaccttc agacagcata 180aatatgtgtg ccatctcatg tgcaattcct tatagaccca gcttggttct tctccaatgt 240ctccttttgg agttgt 256 388 566 DNA Homo sapiens misc_feature (1)...(566) n = A,T,C or G 388ncnagcggcc gcccngncng gnactgaaca ttggtaaaaa attatatgag ggtaaaacaa 60aagaagtcta cgaattgtta gacagtccag gaaaagtcct cctgcagncc aaggaccaga 120ttacagcagg aaatgcagct agaaaaaaac cacctggaag gaaaagctgc natctcaaat 180aaaatcacca gttgtatttt tcagttatta caggaagcan gtattaaaac tgccttcacc 240agaaaatgtg gggagacagc tttcattgca ccgcagtgtg aaatgattcc aattgaatgg 300gtttgcacaa gaatagcnac tggttctttt ctnaaaagaa atcctggngt caaggaagga 360tataagtntt accccctaaa gtggagntgt ttttcaagga tgatgcccat taatgaccnc 420cagtcgggct tgaagaacna cttgattgct gcaaaaattt gcttttcttg gacttcttat 480anggcnaacc tgaaanggat ttcatgaagt catgctacnc aggctatatt tgaaatctgg 540gagaaatcct ggttgcccaa aattgg 566 389 629 DNA Homo sapiens misc_feature (1)...(629) n = A,T,C or G 389actttttttt tttttttttt ttttttgttt tttttttttt tttttttttt ttttttttgc 60agtttctaag tcattacttt tnattttgaa agatttgnga aactnttcac atcatggtga 120gagtttgtat gattaataan aagcagcttt ttcatgaaat gcttggaggt gaacgagttn 180tcagcctgng anatccgacc ntcccattaa ctttgaagtt tctcttgatt aatagaagaa 240aaaaggggag ggtgaanaaa aggaggaaca tgctaaaaac cttatgacaa tcatccaaat 300gtgaggaaag aacaacccga ttcaccaact ccactttttc tattttacaa ctttctacat 360ctcacncttg gattttggcc ttcntggctn aaacantcct ggcantccnt tanagcccct 420gaaaaagagc cntggntttt ncaaaagacn ntnggnnggn gaannccttn annatgccct 480gacccnttcn cnaagaactn nntntccggg ntcccaaaag tttgacccan cagcttantg 540tgaannnaaa actnnccttn aaaggtaatg ggnggaanng gtgannaant gggttttttt 600ganaagtctt ntttttctna aaaccnccg 629 390 596 DNA Homo sapiens misc_feature (1)...(596) n = A,T,C or G 390actttaattt atttcccctt tctagtgtat taagaaatga catgcacttt aatttgccaa 60aagcaatgct tgtattctgg cagcaacatg ctacttctat cacatagtaa agtgaatacc 120agaactacaa aggcaggagg tgtaagtgaa tttttattgg gaggggaggt tggcaactta 180aacagcagca aataaagagt gaataaggaa actccctgtt gccacagata cacaagacct 240ccgtatgtga tacaggagcc atttcaattt gtgaccccta gacagagatg gcaagtgctt 300ttccattcaa tctaatactt ccggattcct actaaaaagg aatcattaag agcatggaaa 360agttgcttac tggaaaggaa acccccgaag agtaagggaa gggaatgtga aattaagaag 420ttatgtggaa tctcttaaat tgnaattact acatttctta atttccaggt atnccaaaca 480cagtccnttg caaaactggt cagntactta aatnccngat ccattttagg cnttacataa 540gtgtttggga gtacctatgg tatttnaatg aacttttaaa cttttntccg ccgtcc 596 391 625 DNA Homo sapiens misc_feature (1)...(625) n = A,T,C or G 391acacacccag gaaatttgtc atccaccctg agagtaacaa ccttattatc attgaaacgg 60accacaatgc ctacactgag gccacgaaag ctcagagaaa gcagcagatg gcagaggaaa 120tggtggaagc agcaggggag gatgagcggg agctggccgc agagatggca gcagcattcc 180tcaatgaaaa cctccctgaa tccatctttg gagctcccaa ggctggcaat gggcagtggg 240cctctgtgat ccgagtgatg aatcccattc aagggaacac actggacctt gtccagctgg 300aacanaatga ggcagnttta gtgtggctgt gtgcaaggtt tccacactgg tgaagactgg 360tntgtgctgg tgggtgtngn canaggacct ngntnctaaa accncgnntt tgggcaatgg 420ggctttcgtc taattnttac aannttgntg accaatnggg gatnaactgg anntttttgn 480tcaanactnt tttggaataa tntccctnnt gcnattngcc ntatttcctg gggaanggtg 540ttnatatngt natggnnaaa cntntanccg nnntntaatc ttggaatata tatnaatacc 600ttcttaaaan ntgntnatta tcctt 625 392 266 DNA Homo sapiens 392ggtacccata ttgctaatgc taggatcaag ataccacata gccagaacaa gaagttgaag 60gtaaacatag aatattttat acaggcactc acacctgcca tttcggaaaa ggattaggaa 120tccagatgcc gtgaatttaa ctattcgtta caggcttgtc ctgcaatatg ctctggagca 180acttgcctgc agagatttct gtatccacgg cttcagagca gaaagagaaa gcaaagaagt 240agagggagga ataaaaatcc ccgcgt 266 393 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 393ggtacttttt tttttttttt tttttttttt tggttttacc tgtttttatt ccttaaaaga 60aaaaaacaac ttaaatgcat acatacagaa tagaatacac ttacttaagt tttgacagtg 120aaaaaaaata attacaggtt agatatttaa tccaaggttt aacatgggga tgatctcata 180aggcaatttc tttcctttaa taaatattaa agtgaatatt attctggaag caaatcatct 240cctaattctt catcagcaaa atcatcctca tcgatccttt tcttggctgc agtttttggt 300cgttctattt gagggccaag tgggtccaca taggaggcat ctatttcttt gntactgcta 360ctttcataag gntcatttgt cccaggtaaa agctctgagt ctggccttan tccgtcaccc 420tttactactg gcnctatagt ctggccacta tnaacgntag ccttncttnt cnttttgnca 480cnggagcccc caatgcannt ttngcntgac tttagcncng gnccctaatt cttcattttt 540ccacctttna gnttttggca antcttgagc cntttttaat cnaagacttn gcanagccaa 600ttaaaaaccc c 611 394 340 DNA Homo sapiens 394acgagtccca ctatgcgctg cccctgggcc gcaagaaggg agccaagctg actcctgagg 60aagaagagat tttaaacaaa aaacgatcta aaaaaattca gaagaaatat gatgaaagga 120aaaagaatgc caaaatcagc agtctcctgg aggagcagtt ccagcagggc aagcttcttg 180cgtgcatcgc ttcaaggccg ggacagtgtg gccgagcaga tggctatgtg ctagagggca 240aagagttgga gttctatctt aggaaaatca aggcccgcaa aggcaaataa atccttgttt 300tgtcttcacg caaaaaaaaa aaaaaaaaaa aaaaagtacc 340 395 557 DNA Homo sapiens misc_feature (1)...(557) n = A,T,C or G 395acacatcttc aaagcacttc cctttaacgg gaaacttagc tttatgggat ttaaacatta 60gaaagtggga aaaaaaattc cattttcttg tcattataaa ccaaaacaaa atctagtgta 120agtcaaggaa actcattcac acttcaggtc cttctcctcc aggaaccagc attgttatat 180tatttccatt tagcaaaatc tgatgtaatt tagtaatcct tcttccttct ggtgtgattt 240caaactcagt gacatcttcc agtactttnt tttttttttt ttttttttgg gtgttgagct 300tggacgcttt cttaattggt ggctgctttt aggcctacta tgggtgttaa atttttactc 360tctctacaag gntttttcct agtggccaaa agaagctggt ccctcttttg gactaccgtt 420aaaattacca nggggattta aaangggtnt tgngggccaa attnaaagtt ngactangan 480tctatttttg gccaaccagt nttaaccagg cttcggtang gttggccgcc cccgggtacc 540ttgggccggg aacacnc 557 396 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 396ggtacngcgg ggccactcga gtgcgcaggc gcctggcgat taccggtctc accatggagc 60ggaaagtgct tgcgctccag gcccgaaaga aaaggaccaa ggccaagaag gacaaagccc 120aaaggaaatc tgaaactcag caccgaggct ctgctcccca ctctgagagt gatctaccag 180agcaggaaga ggagattctg ggatctgatg atgatgagca agaagatcct aatgattatt 240gtaaaggagg ttatcatctt gtgaaaattg gagatctatt caatgggaga taccatgtga 300tccgaaagtt aggctgggga cacttttcaa cagtatggtt atcatgggat attcagggga 360agaaatttgt ggcaatgaaa gtagttaaaa gtgctgaaca ttacacttga aaccagccta 420gatgaaatcc ggttgcttga agtcagttcc aattcagacc ttatggatcc aaatngaaaa 480atggttgtca actactagat gactttaaaa ttcaggagtt aatggaacac atatttgcat 540gggatttgaa gttttggggc anattngtta agnggttctc aaatcaattn ttangggctt 600tcctgccttg ggtnaaa 617 397 594 DNA Homo sapiens misc_feature (1)...(594) n = A,T,C or G 397acgcggggga tcaggactcc tcagttcacc ttctcacaat gaggctccct gctcagctcc 60tggggctgct aatgctctgg gtcccagggt ccagtgggga ccgtcgtggt gactcagtct 120ccggtctccc tgcccgtcac ccttggacag ccggcctcca tctcctgcag gtctggtgaa 180actctccttt acgaagatgg aagcacctac ttgagttggt ttcaccagag gccaggccaa 240tctccgaggc gcctgattta taaagtttct aaccgggact ctggggtccc agacagattc 300agcggcagtg ggtcaggcac ttatttcacg ctgaaaatca acagggtaga ggctgatgat 360gttgggaatt attactgcat gccanggtca aactggcccg tcacttttcg gngaaggacn 420aaaggtggcc natcaaacca actgnggctt gaccattggc ttcatnttcc cgccatttga 480taaccantga aatctggact gctttgtggg ngcctgctga aaacttntat nccnanaggc 540cnaagtcatg acagtttttc natttactcg aaaaatntgg aaatgataat tttn 594 398 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 398acagtggtcc ttttcagagt tggacttcta gactcacctg ttctcactcc ctgttttaat 60tcaacccagc catgcaatgc caaataatag aattgctccc taccagctga acagggagga 120gtctgtgcag tttctgacac ttgttgttga acatggctaa atacaatggg tatcgctgag 180actaagttgt agaaattaac aaatgtgctg cttggttaaa atggctacac tcatctgact 240cattctttat tctattttag ttggtttgta tcttgcctaa ggtgcgtagt ccaactcttg 300gtattaccct cctaatagtc atactagtag tcatactccc tggtgtagtg tattctctaa 360aagctttaaa tgtctgcatg cagccagcat tcaatagtga atggnctctc tttggctgga 420attaccaaac tcagagaaat gnggcatcag gagaacatct taaccccatg aanggataaa 480agccccaaat ggngggnact tgataatagc nctaatgctt taaanatttg gtccactttt 540tacctaaggt gagcccattg aaccannggt gctaaangct catacttcca actgaaatgg 600ttaaggaaaa a 611 399 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 399actctgtgaa tggtgagagg ctgggcacct acatgggcca taccggagct gtgtggtgtg 60tggacgctga ctgggacacc aagcatgtcc tcactggctc agctgacaac agctgtcgtc 120tctgggactg tgaaacagga aagcagctgg cccttctcaa gaccaattcg gctgtccgga 180cctgcggttt tgactttggg ggcaacatca tcatgttctc cacggacaag canatgggct 240accagtgctt tgtgagcttt tttgacctgc gggatccgag ccagattgac aacaatgagc 300cctacatgaa gatcccttgc aatgactcta aaatcaccag tgctgtttgg ggacccctng 360gggagtgcat catnctggcc atgaaaagtg gagagctnaa ccagtattag tgccnnagtt 420tnnanaaggt gttngttnaa tgttaaagga gcantttccg gnagaataac cnacnttcag 480gttattccnn gganatgacc anngtttnga ccccttnnna gtccattaat nccnaacttt 540tttacnctca aattttnaan tnanaaaact tttngnatna aattnttnaa ttanttgttc 600tttttcaata tnnn 614 400 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 400acttacactg tgaaatttta tgatggagta gttcagactg tcaaacatat tcatgtcaaa 60gctttttcca aagatcaggc ctaaagaaac agatcacaaa agtctttcat catctcctga 120taaacgagag aagtttaaag aacagagaaa agcaacagtg aatgtgaaga aagacaaaga 180agataaaccc ttaaagacag aaaagcgacc caagcagcct gataaagaag gaaagttaat 240ctgttctgaa aaggggaaag tgtcagagaa aagtcttccc aagaacgaga aggaagacaa 300ggaaaacatt tccgaaaatg acagagagta ttctggagat gcccaagtgg ataagaaacc 360tgaaaatgac attgtgaaga gtccacaaga aaacttgagg ggaaccnaaa ngaaaacgag 420gcagaccccc ttccatagct nctactgctg gggattnaaa ctttaaactt tggcacccat 480acctttggac ttnnnanaag gaaaatttca nagggtgtga agtcctttaa accgtccttg 540gttgncaaaa ntttttncng ggaaagtcaa aaacttcttt gaaaaccttg ccnangattt 600ttnnggngac nt 612 401 601 DNA Homo sapiens misc_feature (1)...(601) n = A,T,C or G 401ggtacggtaa ctgactccag ggtcactcat actgtgtccg tggtaacggt aagtctgcag 60ctccatcagg atgggcccct tcccagatct acaataggca gcagcaaacc ttgttgcctc 120tcggacgcac aggatatcca ttccatccac tctcagccca ggaatgaaat cgcctctctt 180gtagtaatca gtgctggctg ccgctctctc aacagacgtt cccattccat agcgattatt 240ctcacagatg aaaatacaag gtaatttcca caaagctgcc atgttgtaag cttcgaatat 300ctggccctgg ttagcagcac catcgccata taaagtcagg cagacctcat cttttccatt 360atacttacag gctagagcaa tcccagcgcc caagggcacc tgcgctccta cgatgccatg 420gccccgtana agtcttggca tacatgtgca tcgatcctcc ttcctttagc acaanctcct 480tttgncctgt aactgcaaaa tttntcggac ggaaaggccc cggtgnaaag taaagccgtg 540agcccggnag gctgngatna aanggcttgt ggggttnaag cccggcttca ggtcccacag 600a 601 402 600 DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 402acctggagaa gatcaaacag cgactgtttg agaaccttag aatgctgccg cacgcacctg 60gggtccaaat gcaggcgatt cctgaggacg ccatccctga ggagagtggc cgatgaggac 120gaagacgacc ctgacaagcg catctcgatc tgctcctctg acaaacgaat tgcctgtgag 180gaagagttct ccgattctga agaggaggga gaggggggcc gcaagaactc ttccaacttc 240aaaaaagcca agagagtcaa aacagaggat gaaaaagaga aagacccaga ggagaagaaa 300gaagtcaccg aagaggagaa aaccaaggag gagaagccag aagccaaagg ggtcaaggag 360gaggtcaagt tggcctgaat ggacctnttc agctctggct ttctgctgag tccctacgtt 420ctttcccaac cccttaaatt tataatttct attctctggg gatttatata aaaatttatt 480naatnttaat attcccaggg cccgaaacca agggcccgaa ctnaaggnaa ntttgcttgg 540gtgagctntt tcaagaacca ccttgcaccc atttttccgt cttaacttta accaaaangg 600 403 604 DNA Homo sapiens misc_feature (1)...(604) n = A,T,C or G 403actcagtgga tgacgagtgc ttggtgaaat tgttgaaagg cctgtgtctg aaatacctgg 60gccgtgtcca ggaggccgag gagaatttta ggagcatctc tgccaatgaa aagaagatta 120aatatgacca ctacttgatc ccaaacgccc tgctggagct ggccctgctg cttatggagc 180aagacagaaa cgaagaggcc atcaaacttt tggaatctgc caagcaaaac tacaagaatt 240actccatgga gtcaaggaca cactttcgaa tccaggcagc cacactccaa gccaagtctt 300ccctagagaa cagcagcaga tccatggtct catcagtgtc cttgtagctt tgtgcagcag 360ttccgggctg gaagacagag acagctggac agagctcctg aaaacatttc aaaaataccc 420ccttcccctg gcctgccctg cctttggggt ccancggcac ttcagttgga tggcacaacc 480tantgtatcc gtgcnnaaan cnaacctggc attttcaccc anntanccaa gggcttttgc 540caagggnana acagtggagc ccttggcttg ncctataaac atacgggtac cttggccgnn 600acnn 604 404 604 DNA Homo sapiens misc_feature (1)...(604) n = A,T,C or G 404ggtactttgt ggataagaaa atggaggaac acatctgatg gagagtgggc atttgacaac 60aatggaacag gtaaccagca tgtaaaatca aaatataagt gtctttttaa gagctgaaag 120ctgctgctgg tcattcatta atgtgtcaga catttaatca ggatgctgga ccttcaaaat 180aactgaaaaa agaaccaaga aaaggcgttt ttgttttcaa caaactttac taaataaccc 240cggaaaggca atgaacgatc tgacaattta agctctaatg atttaaagct cagctagaag 300aaagtgaggc atgacatata ctgtcaacgg agggtgaagg aggcagattt ctggaaatgc 360aatgatccca cacatttgct tcaaggagaa acctgcagac atattttcag gtcttgctaa 420gtaacaactg gttatttgta atcaatcatt tgggaaagtc tgctatgtag ctaanggcac 480tgtgaccccn gacaacngat gaaaaggaaa aagcnttgac agcaggaaaa atccttccat 540cttaaagaat ttaggggaca cctttaaagg aaaaaaattg ntccagcctc atttttacaa 600ntnt 604 405 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 405acttgcattt caaagcttat aagatataaa tggagatttt aaagtagaaa taaatatgta 60ttccatgttt ttaaaagatt actttctact ttgtgtttca cagacattga atatattaaa 120ttattccata ttttcttttc agtgaaaaat tttttaaatg gaagactgtt ctaaaatcac 180ttttttccct aatccaattt ttagagtggc tagtagtttc ttcatttgaa attgtaagca 240tccggtcagt aagaatgccc atccagtttt ctatatttca tagtcaaagc cttgaaagca 300tctacaaatc tcttttttta ggttttgncc atagcatcag ttgatcctta ctaagttttc 360atggggagac ttccttcatc acatcttatg ttgaaatcac tttctgtagt caaaggtata 420ccaaaaccaa tttatcttga actaaattct aaagtatggg tatccaacca tatacatctg 480ggtaccaaac ataaatgctg acattcntat attatagtna aggcttaatc nacttgcagg 540tgaatggaaa aaaaataagc ttnaacctag gattctggaa tgaggaatgc tcn 593 406 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 406actttttttt tttttttttt tttttttttg ggactgaatc ttgctctgtc gcccaggctg 60gagtgcagtg gcgcaatctt ggctcactgc aacctctgcc tcctgggttc aagtggttct 120catgcctcag cctcctgggt agctgggatt acagacaagc accaccacaa ccagctagtt 180ttttttgttt tgtttttttg agacggagtc tcgctctgtc accaggctgg agtgcagtgg 240cacaatcttg gctcactgca acctctgcct cctgggttca agagattctc ctgcttcagc 300ctnccaagta gctgggacta caggtgcaca ccatcacacc tggctaattt ttgtattttt 360aagtanagac ggggtttcac catgttggcc aggctggtct caaactcctg acctcaagtg 420aaccggccgc ttancctcca aagtgctggg attacaggcg tgagcccact ggcctggctg 480accatttggt tattaacagg gcccccaana tgcnccttta ngtgaaaggg natggcccca 540gggaacaatt nngctgaaaa acaccaaagg ccnantccat aattcnttgg n 591 407 463 DNA Homo sapiens misc_feature (1)...(463) n = A,T,C or G 407ggtactgatt ttaaaaacta ataacttaaa actgccacac gcaaaaaaga aaaccaaagt 60ggtccacaaa acattctcct ttccttctga aggttttacg atgcattgtt atcattaacc 120agtcttttac tactaaactt aaatggccaa ttgaaacaaa cagttctgag accgttcttc 180caccactgat taagagtggg gtggcaggta ttagggataa tattcattta gccttctgag 240ctttctgggc agacttggtg accttgccag ctccagcagc cttcttgtcc actgctttga 300tgacacccac cgcaactgtc tgtctcatat cacgaacagc aaagcgaccc aaaggtggat 360agtctgagaa gctctcaaca cacatgggct tgccaggaac catatcaaca atggcagcat 420caccagactt caagaattta nggccatctt tcccgggtac ctg 463 408 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 408acaaatatat ataacttaca tttgattgta aggccaacgt tcaaaagtaa aaatgagatg 60agctctctta ttgttatccg aggtcaagag gctgcaactg tcaaggggat gttctcacca 120aaagggggtt tgggggaaga ggacacacac aaagctaata aaaccagaat ccccatcccc 180acaaaactca tgggaacaaa atttaaagga taaaacaaaa cccaccaaga cccatattac 240aaaccaatat ggtaacctgt gttcccttct atggtatgat tatgtcatgt taccttagtg 300ttaaaagatt aacataagga aactgcagca atatataaaa gatatattct ctatagagca 360tatttcgatt gattccatta aaataatgac attagaattc catcatangg ttaaaaccag 420gacaatactg nttttncttt atttaaaaaa aactaccacc taatgactgn attggtcata 480acctgaatgg tgtgcaatgg gctcttccat gaatggctgg cngaaacaag cttgggncct 540gcttgagttt cagctttcct ctttaattta gtngctcaat gataaaca 588 409 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 409ggtacaaaga tctgacatgt cacccaggga cccatttcac ccactgctct gtttggccgc 60cagtcttttg tctctctctt cagcaatggt gaggcggata ccctttcctc ggggaagaga 120aatccatggt ttgttgccct tgccaataac aaaaatgttg gaaagtcgag tggcaaagct 180gttgccattg gcatctttca cgtgaaccac gtcaaaagat ccagggtgcc tctctctgtt 240ggtgatcaca ccaatttttc taggttagca cctncagtca ccatacacag ggtaccagtg 300tcnaacttga tgaaaatcaa gtaatcntgg ccagtctcta aaatcaaatc ttgaatggta 360tcaattcacc cttgatgaag gggaatcggg ggtaacccgg atgggtgccg ggccttnatg 420aagtcancca natgaaggga ttcctttggg gcccccaaag aacttttttn attttcacaa 480cttgnacctt gcccggcggg ccgttcaaaa gggcnaattc cagncacttg gnggccgtct 540aanggatcca actcggacca acttggcgna anatggcaaa ctggttcctg gggaaatggt 600atccctccaa tn 612 410 353 DNA Homo sapiens 410acgcggaagc agtggtaaca acgcagagta acgcgggatg gcacatgcag cacaagtagg 60tctacaagac gctacttccc ctatcataga agagcttatc acctttcatg atcacgccct 120cataatcatt ttccttatct gcttcctagt cctgtatgcc cttttcctaa cactcacaac 180aaaactaact aatactaaca tctcagacgc tcaggaaata gaaaccgtct gaactatcct 240gcccgccatc atcctagtcc tcatcgccct cccatcccta cgcatccttt acataacaga 300cgaggtcaac gatccctccc ttaccatcaa atcaattggc caccaatggt acc 353 411 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 411ggtacgcggg gagagaaacc tggctttact atggcggttg gaggaacggc agtgatcaca 60cgtcggctgc tgggaagatc tggattctcg tttcaggtca ccatcagaaa agctaagttt 120gctgtatagt gaggatcagg agatctgatc ctgattgcag aaccttccct gattacagaa 180tcttgggttg tatctcccac ttcacccttc tagaccatcc cagaagatct ataagatttc 240atctgggaaa tcactaggag ttcttggaag ggaaagaagg aagattgttg gttggaataa 300aaacagggtt gaatgagttc cagaaagcnn ggttctcaac ctcgtggaca gcaatctgca 360gaagangaga acttcaaaaa accnactana agcancttgc anagaagtaa aatgagaagg 420ggncttctna ngaaagaaga cacttggncc acagcagaaa aaactttgac cnantnttnc 480caggaagana gggggggtcc cncttttaaa naaccccctt taagatncng gnggaanacc 540tcanngacca nccntaaatt nnggaaaccg aaaaggggcn gtcctttttg ntnncagntg 600cnccnttaan nt 612 412 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 412acgcggggct ctctcgccag gcgtcctcgt ggaagtgaca tcgtctttaa accctgcgtg 60gcaatccctg acgcaccgcc gtgatgccca gggaagacag ggcgacctgg aagtccaact 120acttccttaa gatcatccaa ctattggatg attatccgaa atgtttcatt gtgggagcag 180acaatgtggg ctccaagcag atgcagcaga tccgcatgtc ccttcgcggg aaggctgtgg 240tgctgatggg caagaacacc atgatgcgca aggccatccg agggcacctg gaaaacaacc 300cagctctgga gaaactgctg cctcatatcc gggggaatgt gggctttgtg ttcaccaagg 360aggacctcac tgagatcagg gacatgttgc tggccaatna ggtgcccagc tgctgcccgt 420gctggtgccc atttgcccat gtgaangtca cttgtgccca gcccaaaaca cttgtcttng 480ggcccganaa gaacttcttt tttccaggcn ttaaaatatt caccccttaa antttcaagg 540ggccccattt gaaatcctgg annatnngca ttgatcaana ttganacaaa gtggnancnt 600ccaaccc 607 413 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 413acaggtcaga gtcttctttt cttttctttt tgagatggag tcttgctctg ttgccagact 60ggagtgcagt ggtgcgatct gggctcactg caatctccac ctcccgggtt caagcgattc 120tcctgcctca gcctcccgag taactgggac tacaggtgtg cgccaccaag cccagctcat 180ttttgtattt ttagtagaga tggggtttca cgatgttggc taggatggtc tcgatctctg 240gtcagagtct tttctgtaaa tatccttggt aaagaagcaa ttttagactg tagctgttgc 300aaatgcttta aggaagaagc anaacaactg tcagtcttcc tgaaatgaag aaactacacc 360agggctgcta tatcagagca accccaacca gcactccaat catgatgccc gacagtggcc 420ccagcttgag aaccagagaa gttccagatg cagagactgt gagctcntga ctatgggaat 480tttngnggcn ntaacccaan tttgagacna aacnaggcct tngncccggt tttnatttgg 540gngggatttt gcggataaan aaacttgnng gggntnctgc ggnatccatg gaacnccaaa 600anatng 606 414 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,C or G 414ggtacttttt tttttttttt tttttttttg tagatgaggt ctcgctatgt tgcccaggct 60ggagtgcagt tattcacagg tgcaaccaca gggcactgca gctttaaact cctgggctca 120agcgatcctc ctgcctcagc ctcccaaata gttgggacta gatgcacgca cnaccacgcc 180tgactcagga cattattctt aaaggtatta tccaggaaac agataaggtc attcataaaa 240cacacggntt ttttctttag ctcagtgtta acaatgaaag tagattccac tattgaagca 300caagttgcaa attggtaaca tagngaacat attgntgtag gaaagggggt tcagtgtgnt 360gtgttatatn agcncttgaa ctttttatgg gngtnataag ccnngttatc ttgncccaaa 420gaaannccat tttnaggatt ngatggtttt cttannggaa nannctnggg ggnattntgt 480ngggcatgaa cttttatgtn ggaatcagtc ccatanaggt aaggggtttn aatcccaaaa 540ancggggnct tttatgggaa atnnccttta cttcaaaggc caaanngatn gtnggtgtca 600cttcnaantt ccngannnca anng 624 415 609 DNA Homo sapiens misc_feature (1)...(609) n = A,T,C or G 415acgcgggtta caacggaagt aaaatctgtc gaaatgcacc atgaagcttt gagtgaagct 60cttcctgggg acaatgtggg cttcaatgtc aagaatgtgt ctgtcaagga tgttcgtcgt 120ggcaacngtt gctggtgaca gcaaaaatga cccaccaatg gaagcagctg gcttcactgc 180tcaggtgatt atcctgaacc atccaggcca aataagcgcc ggctatgccc ctgtattgga 240ttgccacacg gctcacattg catgcaagtt tgctgagctg aaggaaaaga ttgatcgccg 300ntctggtaaa aagctggaag aaggccctaa attcttgaag tctggtgatg ctgccattgt 360tgatatgggt cctggcaagc ccatgtgttg ttgagagctt tctcagacta tccacctttg 420ggtngctttg ctggtcgtga natgagacag acaggtgccn gtggggtggc atcaanncat 480gggacaanaa aggcttnttg gancttgcaa aggtncncaa nttttgncca naagcntcaa 540aagntaattg aatttttccc ctannncctg cncccncttt tannanggnn ggaaaacggc 600ttaaanntt 609 416 577 DNA Homo sapiens misc_feature (1)...(577) n = A,T,C or G 416ggtacgagct gattgggaac gggctccaat ggacatggct ctgcagtcaa aatagttagc 60agatggacag gtttggaaaa tgtgagggcc catatcatca tanccagcaa taaggagacc 120aacaccatat ggtctccggc catatccgtt gtgttggtat ctgggtcttg cttccaatta 180gagatacaag actgagacac aggcagtggt ctatcgaata caaatctgga atncaaacac 240tcctgacgca taaaattaca taacagncta gcatnancag taagcccccg caattgagat 300accaatatgg ttgtcaacat ggagaatttt tttctgatga cctgccaact cttgatttgc 360gcccttttca atgcnaaccc aaaactggca tgaagntttt gnatttcaga ccancctgnt 420ggctgnacct tggcttaaca ggtttccatt ggcntatttc natttggatn aantcttgcc 480cntggggggn ttcnaancta ggggccatca nttggtcaaa ctgntttnta aaccatgggg 540gcnggctcng gccttggttg ctggcntcaa caaaaan 577 417 570 DNA Homo sapiens misc_feature (1)...(570) n = A,T,C or G 417ggtactaaga atattagaga actggaaatc cagttttttt gtggtttttt aagaaagaga 60atctgactcc attgcccagc ttggagagca gtggtgcaat agctggggct acaggcgtga 120gccaccacac caggcctgga aacccagttt taatttgtga actacaaatg gttggcaact 180gattccttaa ttgttattgc aggagtaggc ccaacatgag tccatatgta gtccttctct 240ggtctggtgg gaactgtggg aaatggtgat gaccgtgact tgaaatactn agaaggtgca 300tgacaaacaa attccaagta ttccatcttc cttggaagat cttcctctgg ccctatgata 360taggaagcng gaatcaaatt tgggctcttg ggctaagant aggggtatgg aatgagcccc 420cgtnaantgg cttgnacttc ttcttcgcta atactgggcc ctggattaaa accttttgat 480ttnancnata gntagggctt tccttcttgg ttaatcaatt cccagaaacc aacattccca 540atttgggtaa natactccct tgtanaaaaa 570 418 570 DNA Homo sapiens misc_feature (1)...(570) n = A,T,C or G 418ggtacttcta cacatctgcc taacttggga atgaatgtgg gagaaaatcg ctgctgctga 60gatggactcc agaagaagaa actgtttctc caggcgactt tgaacccatt ttttggcagt 120gttcatatta ttaaactagt caaaaatgct aaaataattt gggagaaaat attttttaag 180tagtgttata gtttcatgtt tatcttttat tatgttttgt gaagttgtgt cttttcacta 240attacctata ctatgccaat atttccttat atctatccat aacatttata ctacatttgt 300aagagaatat gcacgtgaaa cttaacactt tataaggtaa aaatgaggtt tccaagattt 360aataatctga tncagttctt gntatttccc aatagaatgg gactnngnnc tgttaanggc 420ttaagganaa agggaagata agggttaaaa gttggttaat ggacccaacc ntttnaaaga 480aatgcnntan anaatanttt natgantaaa naaaggtncc tngcccnggc cggccgtttt 540aaangggcca atttcnagca cnctnggcgg 570 419 574 DNA Homo sapiens misc_feature (1)...(574) n = A,T,C or G 419ggtacacctt tgactacagc tgcagaagtg ttcctttaga caaagttgtg acccatttta 60ctctggataa gggcagaaac ggttcacatt ccattatttg taaagttacc tgctgttagc 120tttcattatt tttgctacac tcattttatt tgnatttaaa tgttttangc aacctaagaa 180caaatgtaaa agtaaagatg caggaaaaat gaattgcttg gtattcatta cttcatgtat 240atcaagcaca gcagtaaaac aaaaacccat gtatttnact tttttttagg attttttgct 300ttctgtgatt tttcttnttt tttgatactt gcctaacatg catgtgctgt anaantnagt 360taaccaggga aataaccttg ngatnatggc ctanctttta gtttangtct tatgaanttt 420tcattgacca attctaanca ataatggttt annaacaccg tgntntnaaa atttctggta 480anttggaaat aaaaggtttn nttgaaatgg gccttttcca cnnactttnt ttnncagctn 540tttcttggna aataagccct nggttcctga aacc 574 420 573 DNA Homo sapiens misc_feature (1)...(573) n = A,T,C or G 420acctccggta gaattcggtg aatccatctg gtcctggact ctttttggtt ggtaaactat 60tgattattgc cacaatttca gctcctgtta ttggtctatt cagagattca acttcttcct 120ggtttagtct tgggagagtg tatgtgtcga ggaatttatc catttcttct agattttcta 180gtttatttgc gtagaggtgt ttgtagtatt ctctgatggt agtttgtatt tctgtgggat 240cggtggtgat atccccttta tcatttttta ttgngtctat ttgattcttc tctctttttt 300tatntagtct tgctagcagt ctatcaattt ntgtngatcc ttttcanaaa aacccngctc 360ctggaattca tttaatnttt tnaaggggtt ttttngtggc ctctaatttc cttcaagttc 420tggctctgat ttaagttaat atncctggct ttttggctac nttttgnaan gnggttggcn 480cntgnntttt ctanntcctn ttnaantggg gatngnttnn aangcccatt ttnggaannt 540tcccgctttn ntttgggggg catttangtt nnn 573 421 582 DNA Homo sapiens misc_feature (1)...(582) n = A,T,C or G 421ggtacgcggg ggtccgccat ttcgtggacg ccgggtgagt gagagagttg gttggtgttg 60ggccggagga aagcgggaag actcatcgga gcgtgtggat ttgagccgcc gcatttttta 120accctagatc tcgaaatgca tcgtgatttc tgtccattgg actgtaaggt ttatgtaggc 180aatcttggaa acaatggcaa caagacggaa ttggaacggg cttttggcta ctatggacca 240ctccgaagtg tgtgggttgn tagaaaccca cccngctttg cttttgntga atttgaagat 300ccccgagatg canctgatgc aatccgagag ctanattngn angaacacta tgtggcctgc 360ccgtgtnagg aattggaact ggccgnaatg gttgaaanaa agaangttcg aaaattcgtg 420gncctncntt ccttttggng gtcgtcngnc cttnagaatg attaatcgnn nggaaggang 480tccttccncc ttnncccnan antttncant aaangaanaa agcttttttt ngcaacccgn 540aancaggtcc ctttttttag attggganaa atagnngagn tc 582 422 570 DNA Homo sapiens misc_feature (1)...(570) n = A,T,C or G 422ggtactctga ggctttagat tcagtttggg tctttggggg ggacctctat catcacgcct 60ataatcatcc cgagagtaat catctctgga gctccacgac cgatcatccc gtctgtcata 120tcggtcttca tagcggtccc cgcctcctct gtagtcatca tccctgcgat acccactgcc 180aaatgctctt ctgccactgc ctatccggga atcatagcct ctatcatagt ctctgctgcc 240tcggtcatca tagcgatccc ggccaccata tcgatccata tcccggcgtg ggccatccga 300tacccatccc gatacccatc ccgataccgg ctgaatcata acgatctcga tacttgnctc 360caaagctatc atcacctctt ctaggtgggt aagtcatcaa agctgtctgg tagcaaggac 420gaagcccttc aagtctggat ctggtttggg cagaatnccc atttttatca cnggccaaaa 480gnaacgaatc atccctnggc tttaaccnng ngcttgatcn agcaacgtcc acntcgaaat 540tntcctngtt acctananaa ctcttcattg 570 423 584 DNA Homo sapiens misc_feature (1)...(584) n = A,T,C or G 423acccgggtgg ttaaacttcg canaatgcct agatattatc ctactgaaga tttgcctnga 60aagctgttga nccacggcaa aaaacccttc agtcagcacg tgagaaaact gcgagccanc 120attacccncg ggaccattct gatcatcctc actggacgcc acaggggcan gagggtggtt 180ttnctgaagc agctggctag tggcttatta cttgtgactg gacctctggt cctnaatcga 240gttcctctac naagaacaca ccaataaatt tgtcattgcc acttcaacca anantcngat 300atcagcaatg taaaaatncc aaancatctt actgatgctt actttaagaa gangaagctg 360cngaagccca anacancnng gaaggtgaga tctttcgaca canaagtatg agaanttatg 420agatttacgg agcaangcan ggattgatca nganaagctt ngggcctcac caaatttttn 480nccaanannt tcaaagttta ttttcntnag tttcnnnggg cttncttgcn antctggggn 540tggctttgnc ctaatgggaa tttattnctc ccaaaaatgg nggn 584 424 547 DNA Homo sapiens misc_feature (1)...(547) n = A,T,C or G 424actcttggtt tgtcaatggg actttccagc aatccaccca agagctcttt atccccaaca 60tcactgtgaa taatagtgga tcctatacgt gccaagccca taactcagac actggcctca 120ataggaccac agtcacgacg atcacagtct atgcagagcc acccaaaccc ttcatcacca 180gcaacaactc caaccccgtg gaggatgagg atgctgtagc cttaacctgt gaacctgaga 240ttcagaacac aacctacctg tggtgggtaa ataatcagag cctcccggtc agtcccaggc 300tgcagctgtc caatgacaac gggaccctca ctctactcag tgtcacaagg aatgatgtag 360gaccctatga gtgtggaatc cagaacgaat taagtgttga ccacagcgac ccagtcattc 420tggaatgncc tctatggncc aaacgaaccc caccatttcc cctnatacac taattaccgn 480ccaggggtga accttaagct tttctggcat gcagccttta cccacctggc acagtattct 540tggctgn 547 425 567 DNA Homo sapiens misc_feature (1)...(567) n = A,T,C or G 425ggtaccatcc tttaatagat ctcatacacc agaattcaga tcatgaatga ctgacagaat 60attttgttgg gcagtcctga tttaaaacta agactggctt gtggttaaat gaatatgttc 120agtttttgaa ttttaatagt aactccaatt cagtaaatgg tatcactgtt tacccctttt 180aaagatatga ttagacttcg ttagtaatgt tcaacttttc acaaagatgg tgagtgccat 240cttaaaactt actggagatt ggctttatat ttagatttat ataactggtt atgtgaatat 300atttaaatac tggggaaatt gcttcactgt cttagaacca agcaagattc acctgtgttt 360tgtgttcatg ttcatttgcc tcttaaaggc aaggggttga agataaataa ggtagcaatg 420tctatagttt tggccttaac ctatgccaat cctaattata attccctgga nttnaaaang 480gttnctttta ccttatttgg aanggcnttt taaatngngg gttnntgggn naatatttaa 540aggattattc acccctttca catnttn 567 426 563 DNA Homo sapiens misc_feature (1)...(563) n = A,T,C or G 426ggtacaattt gttcaaggaa tttttgtaga aaaatacgat cctacgatag aagattctta 60tagaaagcaa gttgaagtag atgcacaaca gtgtatgctt gaaatcttgg atactgcagg 120aacggagcaa tttacagcaa tgagggattt atacatgaaa aatggacaag gatttgcatt 180agtttattcc atcacagcac agtccacatt taacgattta caagacctga gagaacagat 240tcttcgagtt aaagacactg atgatgttcc aatgattctt gttggtaata agtgtgactt 300ggaagatgaa agagttgtag ggaaggaaca aggtcaaaat ctagcaagac aatggaacaa 360ctgtgcattc ttagaatctt ctgnaaaatc aaaaataaat ggtaatgaga attttttatg 420acctantgcg gcaaattacc ggaaaaactt ccngngcctg ggaaggctng gcaaaaggcc 480ttcatggtca gntgcttaat tatnctaaat gccntgganc ttttgaccag gntctgaana 540actgttgncc aattcaacag ggg 563 427 567 DNA Homo sapiens misc_feature (1)...(567) n = A,T,C or G 427ggtacttttt tttttttttt tttttttttt tttttgttaa aaaccataca tcctttttat 60tgntaagtca taaagaggta tcaaaattaa aagcaaaaat tacagggtaa gacttaacaa 120aactactagg agcgtcaaag gaagtgaaaa tgggactagg cgcggggcaa tatgaattaa 180tgaacatggg aaggacaagg atgggganaa cggtgagcat gtgctgaana tactagggga 240gaggatctgg tgaaaaattt gatcttanac aagcgcctag gtaaagaaat aatgggataa 300gatttctaaa ccccactatg gagcttaaga gtcatcctng ccattggcgc tgtctctgnc 360atcctctcct tcctcaagnc tctttttcat catnctttga tccaattcca gctgggcaat 420tcccccgatc tttnattatc atcatcattc cantanggnn cccnttctta ggaanngntn 480ttttggnccc cccttaanat ttcaatttcc cttnnnccca ttttttttan ggagnttgtg 540gcnntggccc ttttnggntt aaaaatn 567 428 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 428ggtaccctat gaacctgact ctgtggtcat ggcagaagct cctcctgggg tagagacaga 60tcttattgat gttggatnca cagatgatgt gaagaaagga ggccctggaa gaggagggag 120tggtggcttc acagcaccag ttggtggacc tgatggaacg gtgccaatgc ccatgcccat 180gcccatgcct atgccatctg naaatacngc ctttctcata tccactgcca aagggaccat 240canatttcaa tggactgcca atggggacct atcaggcctt tnccaatatt catccacctt 300cagataccag cnactccccc atcgnatgaa tctgnanatg acattaatgc tgataatgaa 360tatctctttn tgcacanatt gttggtcctg gaccccagcc aanaancctt tgcaaanctt 420nctttccaga cctggaggat tacttatnga caccnttgtc cctaaccaga agttgnccat 480ttgngcccng aacancactt tcccaactgg canttngctg gatcccagnn ccttcnggat 540ttggaanaac nttggctttt gatggatttt ttccccgg 578 429 572 DNA Homo sapiens misc_feature (1)...(572) n = A,T,C or G 429ggtaccaaga gtttgctcct ggctgctttg atgtcagtgc tgctactcca cctctgcggc 60gaatcagaag cagcaagcaa ctttgactgc tgtcttggat acacagaccg tattcttcat 120cctaaattta ttgtgggctt cacacggcag ctggccaatg aaggctgtga catcaatgct 180atcatctttc acacaaagaa aaagttgtct gtgtgcgcaa atccaaaaca gacttgggtg 240aaatatattg tgcgtctcct cagtaaaaaa gtnaagaaca tgtaaaaact gtggcttttt 300ctggaatgga attggacata gcccangaac agaaagaacc ttgctgggct ggaggtttca 360cttgcacatc atggaagggt ttagtgctta atctaatttg ggcctcactg gacttngncc 420atttaatgaa gttnantcat tattgnnatc atagtttgct ttgtttnaan ccttnncatt 480taaagttaaa actggaattt nanggtaatt tnaacttgta nggtttcctg ggtttagctt 540tttaaatcnt aatttttcca taagcntttt tg 572 430 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 430ggtacagccc aggtaatttg ctgagcctaa tgggtgtcag ggtcagtcta agtgaaggca 60aagagaggct gggatgaagg gtgcaaagga atagtaaaga aagcatgttt gagatccana 120acagaataat gggtagtaga gggaggtatt gaggatagaa nagtatatgg gtttggcacc 180acggggtgga taggcaaaac atttggttga taangcgcag attctgaact aacttgtaag 240gcttgtctgg ttttaggaca ggtaaaatgg nggaatggta aggagaagtt tataggtttt 300atgagcccat gctgtancan gcaagtgata actngctttt aatccctttt cnaaagcaat 360gcctggngnt atgaagnata tttggcattt gatcngggtt tnaanggntg attagngttn 420ctantgaaca atngnaaagg ggntgccatg atcngtnncc caaggatgng attttanggn 480antctcntac ttgtggggtt naagggtggn gggnttttac naggngggtc cccnaagggn 540gcctnttggn tntangnaat aaanggccng nnaatngana atccnnnttn n 591 431 565 DNA Homo sapiens misc_feature (1)...(565) n = A,T,C or G 431accagtgatg ttttgataca agcatataat gtttaatgat caagtcagga taaatggggt 60atccatcacc tcaagcacat ataatcattt ctttgtatta ggcatattca aattccactc 120ttttagttat ttttaaatat ccagtaaatt agatcttatt cattctatct agatgtattt 180ttgtacttta tttttctcaa atatttttac ttatgctttt tgtcattatc cacagtgttt 240ttttttaaag cctgagccac tttgtggttt cagcctcaat ataataatca tccccttact 300cttagactaa ttccttttcc cctgncactt tgcctgtata ctctgtaaaa atgangacct 360tagaaaatca acatttcctg gtgaactttg agagactatt acaagcagtg cccaaaacag 420tangaataag gcaggtaaaa ccagttggga tagccagatn tattattgat ctggtnggac 480aaanggataa nttggngggc atggtttcca nggcantcgn gaattcccca ttagctttaa 540gggtcnatnn angntggccc anggg 565 432 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 432acgcgggggc caccgtggag agcagagcgc ggcggctgga agctgctaag tcagagccgc 60gatgttccgg attgagggcc tcgcaccgaa gctggacccg gaggagatga aacggaagat 120gcgcgaggat atgatctcct ccatacggaa ctttctcatc tacgtggccc tcctgcgagt 180cactccattt atcttaaaga aattggacag catatgaaga caggacatca catatgaatg 240caccgatatg aagagcctgg ttacagtttc gactcctctc tgnaagtgaa taggcccaga 300aaggtgtaag agactctttg aatggacata aaattctgct tgttnagaac caagttttgg 360ntctgggtna ctgacctttc aaaagctaaa attttaaaac tattttgggg aagtttttta 420tttnnntatt nntcngtttn ttnataaaaa agtaccttgg tnccggnacc acccnttaag 480ggccnaattn cagncnncnt ngngggccgn ttactttnng ggatncntaa nttcggganc 540cnaancttgg ggggtaantc angggtcata nnctggtt 578 433 563 DNA Homo sapiens misc_feature (1)...(563) n = A,T,C or G 433acttcttctg gccaaaggct gttccacatt cactacattt aaaaggcttc tctccaatat 60ggattttctc atgctcagta aggttggatt tgccactgaa ggtttttcca cactccttac 120atacaaaggg cttctctcct gtgtgagttc tctggtgtct gatgaggttt gacttctgaa 180tgaaagcttt cccgcaatct ttacactcaa aaggtttttc tccagtgtga attttctggt 240gcgtaaggag gttttccttc tggctaaatg attttccaca ttcattacat tcgaaaagct 300tctcgccagt atgggtgttc tgatgtttaa tgacatactg cttttggcta aaggcttttc 360cacactcgtt acattcaaaa gggttctctc tccgtgtgaa aatgctcatg ctcantgang 420tttgaattgn nggcttgaag acttttccca tacccttaca ggcaaanggg gttttccccn 480ttggaanatn tntggctgcn tnaagntggt gacatctgga tnggaaacct tttccncatt 540tccaaaggnn tttttttcnn nag 563 434 563 DNA Homo sapiens misc_feature (1)...(563) n = A,T,C or G 434ggtacagctg tctgcattga aaattcatgc atggagaaag ggagtaagca agggagaaac 60ggtgcgattc acatattccg cgagatcatc aagccagcag agaaatccct ccatgaaaag 120ttaaaacaag ataagcgctt tagcaccttc ctcagcctac ttgaagctgc agacttgaaa 180gagctcctga cacaacctgg agactggaca ttatttgtgc caaccaatga tgcttttaag 240ggaatgacta gtgaagaaaa agaaattctg atcgggacaa aaatgctctt caaaacatca 300ttctttatca cctgacacca ggagttttca ttggaaaagg atttgaacct ggtgttacta 360acattttaaa gaccacacaa ggaaacaaaa tcttttcttg aaagaaagta aatngatcca 420cttctggtga atgaatttga aattcaaagg aatctggcct tcatgccanc aaatgggggt 480aattcatgnt ggagaataac ctcctttatc cagccgnaca cacctgttgg aaatggatcc 540aactgctgga aattncttaa taa 563 435 558 DNA Homo sapiens misc_feature (1)...(558) n = A,T,C or G 435ggtacgcggg ggaagatggc ggccgtgcag gcggccgagg tgaaagtgga tggcagcgag 60ccgaaactga gcaagaatga gctgaagaga cgcctgaaag ctgagaagaa agtagcagag 120aaggaggcca aacagaaaga gctcagtgag aaacagctaa gccaagccac tgctgctgcc 180accaaccaca ccactgataa tggtgtgggt cctgaggaag agagcgtgga cccaaatcaa 240tactacaaaa tccgcagtca agcaattcat cagctgaagg tcaatgggga agacccatac 300ccacacaagt tccatgtaga catctcactc actgacttca tccaaaaata taagtcacct 360gcagcctggg gatcacctga ctgacatcac cttaaaggtg gcaggtagga tccttccaaa 420agancttntg ggggaaactn antcttctnt tgaactttca aggaaanggg tgaagtttgc 480agtcatgggc caattccaga aattttaaat cagnagaaga atttttccta ttaataccaa 540ctgggtcggg ggagactn 558 436 528 DNA Homo sapiens misc_feature (1)...(528) n = A,T,C or G 436ggtacaaaaa aaaccttaca taaattaaga atgaatacat ttacaggcgt aaatgcaaac 6cgcttccaac tcaaagcaag taacagccca cgatgttctg gccaaagaca tcagctaaga 12aaggaaactg ggtcctacgg cttggacttt ccaaccctga cagacccgca agacaaaaca 18actggttctt gccagcctct agagaaatcc cagaacactc agccctgaca cgttaatacc 24aaggggaaca gttaactcca atacaaggtc aaaatcagca acaagttcta caatccagtg 30ctgatatcag atacaaagct tcaagggcaa tttcttttcg aaggcttatt ccagtttcgt 36gaggctagca tgaagtgtgt gcatttgcca ggggcaaatt tctattctca attaacccat 42gcagcaaant gctacgcatc tggctgagtc cggtttanaa nccatttgcc ggnggaccaa 48tggaaggggc ccgaattcgt cnnaacttgn cccgggcggg ccgttcaa 528 437 576 DNA Homo sapiens misc_feature (1)...(576) n = A,T,C or G 437actttttttt tttttttttt tttttttttt aggtttgagg gggaatgctg ganattgtaa 60tgggtatgga gacatgtcat ataagtaatg ctagggtgag tggtaggaag ttttttcata 120ggaggtgtat gggttggtcg tagcggaatc gggggtatgc tgttcgaatt cataagaaca 180gggaggttag aantagggtc ttggtgacaa aatatgttgt gtagagttca gggganagtg 240cgtcatangt tgttcctagg aanattgtac nggtgagggt tgtttattat aataatgttn 300gggtatccgg ctntgaaana atngggccaa ngggcctgcg gtgtattcga ngttnaaacc 360tgagactagt tcggactccc ntttgcaagg ncccaaaggg ggttnggttt ggcccttgct 420annggtgnga naataaatcn tntttattgg cccaagggtt cttaacngcn aggagtnaat 480ccaaaggggt ncntnggntt ttnnnanaaa nggttgnnaa aaggttaaag ggacccncct 540ttntnnntaa tgntcgnaat gtcaaatnga tngcnn 576 438 576 DNA Homo sapiens misc_feature (1)...(576) n = A,T,C or G 438ggtaccccaa ttaccagtat ggtggaccct accccttctt ctctgcattg ggaaacagaa 60cagagaacag aaaaaatcat tccatcttgc tcttaactct ttccacctat gtgctcagtt 120tttcaagtag aatttctatt cctttgctgg tgcttttggt tttttccaat gtaggaatca 180agcttttcag tgcagctttg actttgtttg caacttccag gtcacaactc tggaggaggc 240tagaaagaat aatggcacct cgatttacac tagcccagga cttcaggttc ttcataccaa 300catgctctac aagtgttttt gcaaaacaac cttctcttcc attntctttt catcttttta 360tcttgctcta ttaaccactt nagaaactaa gaatgtccct gcaaggatgt tctggcaatg 420ntgaaagctt ctccgtcctt ggccaccagg atgcaagtcc ntggttnttg ccagcttggc 480cnatnggcat tccatnggna nggcttgaac cgttttccag ggggcagant cccaaaatgg 540ccngacacca acccnacang cagacttntt ttagcn 576 439 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 439cgaggtacgc gggggagaaa aaacctgcgg aaaatggtag cgatggcggc tgggccgagt 60gggtgtctgg tgccggcgtt tgggctacgg ttgttgttgg cgactgtgct tcaagcggtg 120tctgcttttg gggcagagtt ttcatcggag gcatgcagag agttaggctt ttctagcaac 180ttgctttgca gctcttgtga tcttctcgga cagttcaacc tgcttcagct ggatcctgat 240tgcagaggat gctgtcagga ggaagcacaa tttgaaacca aaaagctgta tgcaggagct 300attcttgaag tttgnggatg aaaattggga aggttccctn aagtccaanc ttttgttang 360agtgataaaa cccaaactgt tcagaaggac tgccaaatna aagtatgtnn cgtggtttca 420aaccntgaat taaaaggctt ttngaccaac atngggnaca attgcttgan nacttgtcca 480tttcttaaaa ttgggaacnc tggaccnggt nanaaanatt tcngattgga aaantttgga 540ccncatttta aatcttgctt aaattttggc caatcctt 578 440 573 DNA Homo sapiens misc_feature (1)...(573) n = A,T,C or G 440ggtacttttt tttttttttg agacagggtc ttgccctgtc acccaggctc gagtgcactg 60gagtgatcac agctcactgg cctcaagtga tcctcctgcc ttggcccctt aagtgccagg 120gttacaggca tgagctacca tgcctggcag aaattcaaga tttggataaa cttacttctt 180tgccaagcct gttcttcaag ttattcagaa ctgggtgtat accttgtcct catatgtatc 240ttgtccctgc tgtcttttag gttagcaagg tgtatgaata cttttaagtt ttgtttgttc 300ttttcctcgt ggtatcaagt gaaatactga tctattctct ggctagggtc aatttacaaa 360attgccatgg aactgagcca aaaggcccca cgtgggataa aaattnctta ccatcgacgc 420ccanccgtan tttttcaagg tattggcttt tggaagnttt accaaatttc nggtaaacca 480aaattcnaaa agnaaaaaat tnccctggng taaccttgcc cgggcggccg ttcaaaaggg 540cnaatttcca ncacattggg cggccgttaa tna 573 441 572 DNA Homo sapiens misc_feature (1)...(572) n = A,T,C or G 441ggtacaaaat tttattaaag gtctttagag agcaacatcc agactccaga atacagctgc 60caaggagacc ctgttatgct gtggggactg gctggggcat ggcaggcggc tctggcttcc 120cacccttctg ttctgagatg ggggtggtgg gcagtatctc atctttgggt tccacaatgc 180tcacgtggtc aggcaggggc ttcttagggc caatcttacc agttgggtcc cagggcagca 240tgatcttcac cttgatgccc agcacaccct gtctgagcaa cacgtggcgc acagcagtgt 300caacgtagta gttaacaggg gtctccgctt gtggatcatc aagccatcca caaacttcat 360ggatttagcc ctctgncctt cggaggttcc cagacaccca caanctngca agcctttggc 420cccacttttc catgatgaaa ctgnagncac aaccatangc aagggccctt cggacannta 480aggccttcct aaggagnttg naacncnana naacttttgc ttgggcantg ggcacaccag 540nacctntaag nggccccctt tttaagcata aa 572 442 562 DNA Homo sapiens misc_feature (1)...(562) n = A,T,C or G 442acaggtcaga gtcttctttt cttttctttt tgagatggag tcttgctctg ttgccagact 60ggagtgcagt ggtgcgatct gggctcactg caatctccac ctcccgggtt caagcgattc 120tcctgcctca gcctcccgag taactgggac tacaggtgcg cgccaccaag cccagctcat 180ttttgtattt ttagtagaga tggggtttca cgatgttggc taggatggtc tcgatctctg 240gtcagagtct tttctgtaaa tatccttggt aaagaagcaa ttttagactg tagctgttgc 300aaatgcttta aggaagaagc aaaacaactg tcaagtcttc ctgaaatgaa gaaactncac 360cagggctgct atatcagaac aaccncaacc aagcacttca aacatgatgc cgacaggtgg 420ccccagctta aaaaaccagg aanaagttcn gantcccnaa actgngaatg cctcttggac 480ttttggaatt aattgggggc cagtagccaa gttatnagac caaatcangg cntagggccc 540cgtattattt ggcggggatt tg 562 443 585 DNA Homo sapiens misc_feature (1)...(585) n = A,T,C or G 443acttttattt tttggtggtg aaattgactg atgattttcc tttttcttcg ctggactatt 60gtgccaactg ccaggctgcc tcctgccctt acagccctaa gtggctgcct tctttccatc 120aactcccaac ttcttcctgt gaagtttaat tgtctcaacg cctccccctc ccccattccc 180tccatttttc tcccaagaaa cctgactcaa ttatttgcat attttgagaa actgctgcag 240attagttctt tttgccagtt ttccctggaa ctcctggcct tttgtggagg ggagggatgg 300agagaatagg aatcttcact agaagccgtg ggaagaattg gaagttacat gctgtatatg 360caatgtccag cagtctgata aactgacgat tcttaatcaa gattttttcc tgatggggaa 420gggactttta ttttctttta nagaggggaa agtgtgagct cttcccttat tcctaatggc 480tatttttgaa gcaaanaagg ccacaacatt ngcacatgcc acctgcnaag gaccttgagt 540nagtgaagnc tcctaaaact gggttaanaa ccttgttttc tctnn 585 444 437 DNA Homo sapiens misc_feature (1)...(437) n = A,T,C or G 444acgcggggac gtgactcagc actttcccca gagcccggac tgcggagaac aatatcctcc 60tccctaacag ataaacagcc cttgttcctc gggataagga ctggcagtcc cctgacaccc 120taagaccggc atctgtcgat gttatttccc cagcatggcc gaaacagaag ccctgtcgaa 180gcttcgggaa gacttcagga tgcagaataa atccgtcttt attttgggcg ccagcggaga 240aaccggcaga gtgctcttaa aggaaatcct ggagcagggc ctgttttcca aagtcacgct 300cattggccgg aggaagctca ccttcgacga ggaagcttat aaaaatgtga atcaagaagt 360ggtggacttt gaaaagttgg atgactacgc ctctgccttt caaggtcatg atgttggatt 420ctgtgcctgg gtacctn 437 445 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 445actttttttt tttttttttt tttttttttt taaggtttga gggggaatgc tggagattgt 60aatgggtatg gagacatatc atataagtaa tgctagggtg agtggtagga agttttttca 120taggaggtgt atganttggn cgtagcggaa tcgggggtat gctgttcgaa ttcataagaa 180cagggaggtt aaaagtaggg tcttggtgac aaaatatgtt gtgtanagtt caggggaaag 240tgcgtcatat gttgttccta ggaanattgt antggtgagg gtgttaatta taataatgtt 300tgtgtattcg gctatnaana atagggccaa atgggcctgc ngcctattcn atgtttaanc 360tgagacttnt tcggactccc cttcggcaan gtcnaantgg ggttcggttg ngcnctgcag 420tgnggagata nntcntntta ntggccaatg gtnnngatgg ccagaataat cannanggnt 480tcnttnntcn tnaaaaggtc naaatggttn angganaccn cttattagga attgttaatc 540ttnaatgatn gttntggnga cnctatatgg anaatgtnag gnctactccn ng 592 446 599 DNA Homo sapiens misc_feature (1)...(599) n = A,T,C or G 446ggtacggcaa acacaacgga cctgagcact ggcataagga cttccccatt gccaagggag 60agcgccagtc ccctgttgac atcgacactc atacagccaa gtatgaccct tccctgaagc 120ccctgtctgt ttcctatgat caagcaactt ccctgaggat cctcaacaat ggtcatgctt 180tcaacgtgga gtttgatgac tctcaggaca aagcagtgct caagggagga cccctggatg 240gcacttacag attgattcag tttcactttc actggggttc acttgatgga caaggttcat 300agcatactgt ggataaaaag aaatatgctg cagaacttca cttggttcac tggaacacca 360aatatgggga ttttgggaaa gctgtgcagc aacctgatgg actggccgtt ctaggtattt 420tttttgaagg ttggcagcgc taaaccnggc cttnataaag ttgttgaatg tgctggattc 480cattaaaaca aagggcaaga attgctgact ttcactaatt nnaatcctcg tnggccttct 540tcctgaaatc cttggattac cggacctncc cagcttactn accanccttc tcttttngg 599 447 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 447ggtacgcggg atgagtgtgg aatccagaac aaattaagtg ttgaccacag cgacccagtc 60atcctgaatg tcctctatgg cccagacgac cccaccattt ccccctcata cacctattac 120cgtccagggg tgaacctcag cctctcctgc catgcagcct ctaacccacc tgcacagtat 180tcttggctga ttgatgggaa catccagcaa cacacacaag agctctttat ctccaacatc 240actgagaaga acagcggact ctatacctgc caggccaata actcagccag tggccacagc 300aggactacag tcaagacaat cacagtctct gcggagctgc caagccctcc atctccagca 360acaactccaa acccgtggag gacaaggatg ctgtggcctt ccctgtgaac ctgaggctca 420gaacacaacc tacctgtggt gggtaaatgg tcagagcctc cagcagtccc aaggctggag 480ctgtccaatg gcaacangga cctnactcta ttcaatgtca caagaaatga cncaagaacc 540tatgnatgtg gaatccagaa ctnagtgatg caaaccgaat gaccagnn 588 448 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 448accatttgtc tgacctctgt aaaaaatgtg atcctacaga agtggagctg gataatcaga 60tagttactgc tacccagagc aatatctgtg atgaagacag tgctacagag acctgctaca 120cttatgacag aaacaagtgc tacacagctg tggtcccact cgtatatggt ggtgagacca 180aaatggtgga aacagcctta accccagatg cctgctatcc tgactaattt aagtcattgc 240tgactgcata gctctttttc ttgagaggct ctccattttg attcanaaag ttagcatatt 300tattaccaat gaatttgaaa ccagggcttt tttttttttt ttgggtgatg taaaacncaa 360ctncctgnca ncaaaataat taaaatagnc acattgntat cttttattag gtaattcact 420tcttaattan atggntcaat actctaagna tcaaaatntt ccaattatna tggctcacct 480gaaagaagna tgctctttta aggaatacag cttcttcnat tnacaattta acanggggag 540aaaattaaan tnaangantt ganatctgga ggngtannaa ngntctcgcn ttc 593 449 577 DNA Homo sapiens misc_feature (1)...(577) n = A,T,C or G 449actgtgggtc gaagtaatgg atacggacgt aaccatcttc gccgccgctg ctgtagctct 60tgccatcagg atggaaggca acactgttga taggtccaaa gtgacccttg actcttccaa 120actcttcttc aaaggccaaa tggaagaacc tggcctcaaa cttgccaatc ctggtggagg 180ttgtggttac atccatggct tcctgaccac cgcccaggac cacatggtca tagttggggg 240agagggcagc tgagttgaca ggacgttctg tccggaaagt cttctgatgt tcaagagttg 300tggagtcaaa aagcttggct gtgttgtcct tggacncggc acaaacatgg tcatgtccct 360ggataactgg atgtcgttga tctgccggga gtgctcctta acattcacca acacctcttc 420anacttggca ctatactggt tgactctcca ctcttatggc cnggatgatg cactccccca 480aggggtncca aacagnactg gtgatttaga atcattgcan ggatcttatg tagggctcat 540tgntgcaatc tggcttggat ccgcagtcaa aaaagnt 577 450 575 DNA Homo sapiens misc_feature (1)...(575) n = A,T,C or G 450ggtacttgtg atcacactac gggaatctct gtggtatata cctggggcca ttctaggctc 60tttcaagtga cttttggaaa tcaacctttt ttatttgggg gggaggatgg ggaaaagagc 120tgagagttta tgctgaaatg gatttataga atatttgtaa atctattttt agtgtttgtt 180cgttttttta actgttcatt cctttgtgca gagtgtatat ctctgcctgg gcaagagtgt 240ggaggtgccg aggtgtcttc attctctcgc acatttccac agcacctgct aagtttgtat 300ttaatggttt ttgtttttgt ttttgtttgt ttcttgaaaa tgagagaaga gccggagaga 360tgatttttat taattnttnt tttttttttt tactatttat agctttaaaa agggcctncc 420ttcccctctt ctttctttgg nctctttcat taaccccttc ccagtttttt ttaacttaaa 480ccccgttctc atggcctngg ccttttgaag cgnttcctct tataaaaagc tttgccgaac 540aanttttttt taccgatccc aaatttatga agggg 575 451 573 DNA Homo sapiens misc_feature (1)...(573) n = A,T,C or G 451actaggctaa ctagaaggat ctcatcccca tatgtggtct catttcaagt ctatggatga 60ctaccttcat tgctgtgtgc gagatggttt caccccttga aaatatggtc acttcagcat 120aaaatagtta aatctttata atgatcaatt catcctacct ccttttacat gcagctgaaa 180aatgacaggc tagggacata gaatattgtg aactttatac tgttagaatc actgtccatt 240aaatgatcac tagctaatgg tcactaaatt tacaaattaa ggaaattata tatagaatac 300tgcaaaaaca cagtaaaaag actgaagttc gcccatttct gctcaggaag tctcttcact 360cctaagcttc atatgttgcc ttctggcttc aaaattctgc tattattact gttttcctcc 420tttgatcttc ctttggtccc cagtgccaga cttccaagcc ttttngttaa aaagccatct 480tttggatgcc atttcnaaca gcttcagtga tgcctctgaa aaaaggatct gccggctaan 540atttctcngg ttcgtgcttt ctaccgganc tcc 573 452 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 452acaattttat ccctaaaact ctgttgacat caaaatatga cagttgctat atccataaaa 60tatttacata gcacggcata ttaagcttta gacacttggc aattaaacca cataaaaaga 120ggacaagacc cccatcctac atgtttggaa tcaggtgttc accggtccct atctggcgac 180tgtacgcggg tggggtcctt acttgtattc tgttatcagc tgattttgaa acatataata 240atgattttct tgttcccttc tttaactagc tgcctttaga ttttgataat cacagtctta 300aaatactagg aaagaagtgg atgggaattg taggcataga tttcatatca agggcatttc 360aagacagaat ttttaattcc tgtagtaggc ttgctggagc naaaggaaaa tgtgctggtt 420aaaaatcaac ttatgccatt ttaaaatttg ataaaatttg gagtggcatn ctgctaaggg 480gagaccttgg gccggacccc cttangggca aattccngca cactgggggg cggtactang 540gggatccgac ntcggnccan acttggcgna tcatgggctt antgttcctt gnggn 595 453 380 DNA Homo sapiens misc_feature (1)...(380) n = A,T,C or G 453ggtacgcggg gagccgcctg gataccgcag ctaggaataa tggaatagga ccgcggttct 60attttgttgg ttttcggaac tgaggccatg attaagaggg acggccgggg gcattcgtat 120tgcgccgcta gaggtgaaat tcttggaccg gcgcaagacg gaccagagcg aaagcatttg 180ccaagaatgt tttcattaat caagaacgaa agtcggaggt tcgaagacga tcagataccg 240tcgtagttcc gaccataaac gatgccgacc ggcgatgcgg cggcgttatt cccatgaccc 300gccgggcagc ttccgggaaa ccaaagtctt tgggttccgg ggggagtatg gttgcaaaaa 360aaaaaannaa aaaaaaaagt 380 454 589 DNA Homo sapiens misc_feature (1)...(589) n = A,T,C or G 454ggtactcttg gtttatcaat gggacgttcc agcaatccac acaagagctc tttatcccca 60acatcactgt gaataatagc ggatcctata tgtgccaagc ccataactca gccactggcc 120tcaataggac cacagtcacg atgatcacag tctctggaag tgctcctgtc ctctcagctg 180tggccaccgt cggcatcacg attggagtgc tggccagggt ggctctgata tagcagccct 240ggtgtatttt cgatatttca ggaagactgg cagattggac cagaccctga attcttctag 300ctcctccaat cccattttat cccatggaac cactaaaaac aaggtctgct ctgctcctga 360agccctatat gctggagatg gacaactcaa tgaaaattta aagggaaaac cctcaggcct 420gangtgtgtg ccactcagag acttcaccta actagagaca gtcaaactgc aaccatgggt 480gagaaattga cgacttcaca ctatggacag cttttnccaa gatgtcaaac aagactcctc 540atcatgataa ggntcttacc cctttaattg nccttgttat gcctgccct 589 455 589 DNA Homo sapiens misc_feature (1)...(589) n = A,T,C or G 455ggtacgcgga agagacaggg tttcaccatg ttgcccaggc tggtttcgaa ctcctgacct 60caggtgatcc acccgcctcg gcctcccaaa gtgctgggat tacaggcttg agcccccgcg 120cccagccatc aaaatgcttt ttatttctgc atatgttgaa tactttttac aattcaaaaa 180aatgatctgt tttgaaggca aaattgcaaa tcttgaaatt aagaaggcaa aaatgtaaag 240gagtcaaaac tataaatcaa gtatttggga agtgaagact ggaagctaat ttgcattaaa 300ttcacaaact tttatactct ttctgtatat acattttttt tctttaaaaa acaactatgg 360atcagaatag ccacatttag aacacttttt gttatcaagt caatattttt agatagttag 420aacctggtct taagcctaaa agtgggcttg attctgcagt aaatcnttta caactgcctc 480gacacacatt aaccttttta aaaatngacc ttcccgaagt cttttggtag catggnacac 540ctgatgctta natgttcang taattaatat ggnccagnag tnttgtnnc 589 456 582 DNA Homo sapiens misc_feature (1)...(582) n = A,T,C or G 456acagaatgtt gatacaaagc ttaaaattct tgcatatggt catagaaaat gcatctttgg 60ttttgtgttt ttatcacttg cttccaactt aggcttttgg ctcagaagat tattgaataa 120tgatttgtct tagtttctgt ttcagtaagg gaattctgag gccgttgcta tgataccatc 180attaagacat tcacatgtct tcatataata tctcttcatt tcaaatccta atcactattt 240catactatta cagggctttg atgctgccag cactgtcttt tacataggaa attctagatt 300tgcacagtaa tagaggaatt agaagtacct aactatacac tttgattcag cctgctaaat 360caggggttca atactagctt ggacaaactt tgtaagtaat taattgctac cagccttatt 420ggaaacaaat tatcaactag tttcccctgc caaattttga aattcactgn ttcacttaat 480ctattatatt actaataatg gattaataaa gatgaattaa ttattattac ttactagtnt 540aaatgaaaaa cagggactga aatagtctgn atccgngttg ca 582 457 380 DNA Homo sapiens 457ggtacttttt tttttttttt tttttggagt ttttagttta ttaatgttct tgcgaaaaat 60ccacagtggc cacagctaac atcattgcag cacctttact ccttcggctg tgatccaatc 120tccagctcac ttctttttgc cagcaccaac attggccttt gcagtccccc tgactttctt 180cattctgttc ttgcgttcct ttcgttgctt tcttgaggtc tttttcttct catacaggcc 240atgtcttgca agtctatgtt tgggttcatt tttctttgca taatccaggg aatcataaat 300catgccaaag ccagttgtct tgccaccacc aaaatgagtt ctgaatccaa atacaaagat 360gacatccggt gtggtcttgt 380 458 382 DNA Homo sapiens misc_feature (1)...(382) n = A,T,C or G 458acgcggggag aacagccacc cctctctcgg gcactgctgc catgaatgcc ttcctgctct 60ccgcactgtg cctccttggg gcctgggccg ccttggcagg aggggtcacc gtgcaggatg 120gaaatttctc cttttctctg gagtcagtga agaagctcaa agacctccag gagccccagg 180agcccagggt tgggaaactc aggaactttg cacccatccc tggtgaacct gtggttccca 240tcctctgtag caacccgaac tttccagaag aactcaagcc tctctgcaag gagcccaatg 300cccaggagat acttcagagg ctggaggaaa tcgctgagga cccgggcaca tgtgaaatct 360gtgcctacgc tgcctgtacc tn 382 459 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G 459ggtactgagg aaatattttg taaagtgagc tttgggtata acttagcccc atcattattt 60agagaataga ggaggaagaa agaggaagga ttttaaaggc agacaatgac agaccattca 120ggataggtag ggttttaaag ggagataaac acagtctcat caactaagga gagatttgct 180gcagtaaata ggatgaggga aatagtctgt gggatgcaag caaaggaagc agggtgcctt 240agacactgag tggagccaga aagatcatgc ggcctttttc caagtacatg gccaccaagt 300aagaatggtt ggtgacaaga cagaaggcta aaacaggaag gtaatcttgt gcacctgaca 360aatngaaaga attaaggatc aaaattgaag caggctntaa gagtttcaag aaattcttaa 420aacccaaaag tgatttggaa gccccaaact ttccggtaat gctncccatg gcatgatggg 480ccaaaacctt gggggttcct aagttnnaaa agccctntnc caaattttaa tggaccccct 540acattttttc taatcaatcc cccctttcca aaaaaatngg acctcntttt tt 592 460 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 460acgcgggcac tatcctgaat tatgtgcctg tctagataag cagagaccat gccaaagcta 60taatggaaaa caagtttaca aagagacctg tatttctttc ataaaagact tcttggcaaa 120aaatttgatt atagttattg gaatagcatt tggactggca gttattgaga tactgggttt 180ggtgttttct atggtcctgt attgccagat cgggaacaaa tgaatctgtg gatgcatcaa 240gctatcgtca gtcaaacccc tttaaaatgt tgctttggct ttgtaaattt aaatatgtaa 300gtgctatata agtcaggagc agctgtcttt ttaaaatgtc tcggctagct agaccacaga 360tatcttctag acatattgaa cacatttaag atttgaggga tataagggaa aatgatatga 420atgtgtattt ttactcaaaa taaaagtaac tgttacgttg cgaaaaaaan nnnnnnnnnn 480naaaaaaaag tnccttgggc cgggaccacg ctagggcaaa tccagcacac tggcggccgt 540actagggatc cactnggacc agctggcgna atatggnn 578 461 425 DNA Homo sapiens misc_feature (1)...(425) n = A,T,C or G 461acgcggggct ttctggtctc ggccgcagaa gcgagatgac gaagggaacg tcatcgtttg 60gaaagcgtcg caataagacg cacacgttgt gccgccgctg tggctctaag gcctaccacc 120ttcagaagtc gacctgtggc aaatgtggct accctgccaa gcgcaagaga aagtataact 180ggagtgccaa ggctaaaaga cgaaatacca ccggaactgg tcgaatgagg cacctaaaaa 240ttgtataccg cagattcagg catggattcc gtgaaggaac aacacctaaa cccaagaggg 300cagctgttgc agcatccagt tcatcttaag aatgtcaacg attagtcatg caataaatgt 360tctggtttta aaaaatnnan nnnaannntn ntnnaaanaa aaaaagtnct nggccgngac 420cacgc 425 462 581 DNA Homo sapiens misc_feature (1)...(581) n = A,T,C or G 462ggtactattg acccagcgat gggggcttcg acatgggctt tagggagtca taagtggagt 60ccgtaaagag gtatctttac tataaaagct attgtgtaag ctagtcatat taagttgttg 120gctcaggagt ttgatagttc ttgggcagtg agagtgagta gtagaatgtt tagtgagcct 180agggtgttgt gagtgtaaat tagtgcgatg agtaggggaa gggagcctac tagggtgtag 240aataggaagt atgtgcctgc gttcaggcgt tctggctggt tgcctcatcg ggtgatgata 300gccaaggtgg ggataagtgt ggtttcgaag aagatataaa atatgattag ttctgtggct 360gtgaatgtta taattaagga gatttgtaag ggagattagt atanagaggt anagtttttt 420tcgtgatagt ggntcactgg ataantggcc gttggctttg ccatgattgt gaggggtagg 480agtcaagtag ttagtattan gangggggtt nttaggggtc cnaggaaang ttggggaana 540ctaaannggt gtngtnattn gtaaaaaata nnnnanggat n 581 463 574 DNA Homo sapiens misc_feature (1)...(574) n = A,T,C or G 463actgtgtggc gccttattct aggcacttgt tgggcagaat gtcacacctg ccgatgaaac 60tcctgcgtaa gaagatcgag aagcggaacc tcaaattgcg gcagcggaac ctaaagtttc 120agggggcctc aaatctgacc ctatcggaaa ctcaaaatgg agatgtatct gaagaaacaa 180tgggaagtag aaaggttaaa aaatcaaaac aaaagcccat gaatgtgggc ttatcagaaa 240ctcaaaatgg aggcatgtct caagaagcag tgggaaatat aaaagttaca aagtctcccc 300agaaatccac tgtattaagc aatggagaag cagcaatgca gtcttccaat tcagaaccaa 360aaaaaaaaaa naaaaaaaag tacttttttt ttttnnnnnt tttttttttt taggtaatgg 420gtgttgagct tgaacgcttt cttaattggn ggctgctttt angcctctat gggtgttaaa 480ttttttactc tcttacaagg tttttcctaa gtccaaanac tgtccttttg gctacagtta 540aatttccagg ggattaaagg gttttgggcn aatt 574 464 580 DNA Homo sapiens misc_feature (1)...(580) n = A,T,C or G 464ggtacctagt aagctctccc tcctcccacc ctccaccctc aaggaggccc cagtgtcagt 60tgttcccctc tgggtccatg agttcttatc atttagctcc cacttataag caagaacatg 120cagtatttgg ttttctgttc ctgccttagt ttgctaagga taacggcctc cagctccatc 180cagttcctgc aaaggacatg atcctgttct ttctatggct gtatagtatt ccatggtgta 240tatttaccac attgtcttta tccagtctgt cattgatggg cttttgggtt gattagtagc 300tttttgaatg gtaacttttc tacagaagta cgcggggctt ttttttttgc tgtaggcccg 360ggtggttgct gccgaaatgg gcangttcat gaaacctggg aaggtggtgc ttgtcctgct 420ggacgctact ncggacgcaa agctgtcatc gtgaaagaac attgatgatg gcaccttana 480cgccctacag ccatgctctg gtggctggaa ttgaccgcta cncccgaaag tgacagctgn 540catgggcaag aagaagatcg ccagagatca aagataaaan 580 465 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 465ggtacttttt tttttttttt tttttttttt ttctacatca ctttanaata tttattgtat 60tccttaatgc atttcttaac atgtatagca ctctttaatc aagaatataa agtcatctac 120ttagaatcac attatcttaa agatgcatac tggaatgata agtttgaaga tgtaactatc 180aacaattctt ttcaaaatca tatcaatata ttactctcat ggaacttgca cattctaaga 240agggtcattt tttcccccca gtaccaatat tacattattt gacagggata ataaaatgag 300cagagactgg aaatcacaga caataacatt gctttctcaa ttaacagaaa ggattcataa 360catattcctt aacggtagat gtgatttgta gagaatgtgg aaaagaacta ttgagaagtc 420cacctgctgc ccaaactgag gcacattagg gtggttgtgg gangagttat atttgagggt 480ccatttttcc ttagggttta aaagcatgtc cnggttggng gtnatttgcc attaagtctn 540ttttcaaata aaagaattag gggagaaagt ttggaaaa 578 466 546 DNA Homo sapiens misc_feature (1)...(546) n = A,T,C or G 466accaatacca ccaattttgt agacatcctg gagaggcagg cgcaagggct tgtcagttgg 60acgagttggt ggtaggatgc agtccagagc ctcaagcagc gtggttccac tggcattgcc 120atccttacgg gtgactttcc atcccttgaa ccaaggcatg ttagcacttg gctccagcat 180gttgtcacca ttccaaccag aaattggcac aaatgctact gtgtcggggt tgtagccaat 240tttcttaatg taagtgctga cttccttaac aatttcctca tatctcttct ggctgtaggg 300tggctcagtg gaatccattt tgttaacacc gacaattagt tgtttcacac ccagtgtgta 360agccagaang gcatgctctc gggtctgccc attcttggag ataccagctt caaattcacc 420aacaccagca gcaacaatca ggacagnaca gtcggnctga gatgtccctg taatcatgtt 480ttgataaaag tctctgtgtc ctggggcatc aatgatagtc acatagtacc tcggccgcga 540ncacgc 546 467 445 DNA Homo sapiens misc_feature (1)...(445) n = A,T,C or G 467acctaaaacc cgaagaacct tctgtaagaa gtgtggcaag catcagcctc acaaagtgac 60acagtataag aagggcaagg attctttgta tgcccaggga aggaggcgct atgatcggaa 120gcagagtggc tatggtgggc agacaaagcc aattttccgg aagaaggcta agaccacaaa 180gaagattgtg ctaaggctgg aatgtgttga gcctaactgc agatccaaga ggatgctggc 240tattaagaga tgcaagcatt ttgaactggg aggagataag aagagaaagg gccaagtgat 300ccagttctaa actttgggat atttttcttc aattttgaag agaaaatggt gaaccataga 360aaagttaccc gagggaaaat aaatacagtg atattccaaa aaaaaaaann nnnnnaaaaa 420aaagtncttg gccgggaccc cctaa 445 468 566 DNA Homo sapiens misc_feature (1)...(566) n = A,T,C or G 468actgtgtggc gccttattct aggcacttgt tgggcagaat gtcacacctg ccgatgaaac 60tcctgcgtaa gaagatcgag aagcggaacc tcaaattgcg gcagcggaac ctaaagtttc 120agggggcctc aaatctgacc ctatcggaaa ctcaaaatgg agatgtatct gaagaaacaa 180tgggaagtag aaaggttaaa aaatcaaaac aaaagcccat gaatgtgggc ttatcagaaa 240ctcaaaatgg aggcatgtct caagaagcag tgggaaatat aaaagttaca aagtctcccc 300agaaatccac tgtattaagc aatggagaag cagcaatgca gtcttccaat tcagaaccaa 360aaaaaaaaaa nnaaaaaaag tacttttttt tntnnnnnnn ttttttttag gaatgggtgt 420tgaacttgac ctttcttaat gggggctggt tttaggctat atggngtaaa tttttctctt 480ttacaaggtt tttcctagng ncaaaaactg tcctttggac taccgtaaat tacaggggtt 540taaaggttnt ggggcaatta aanttn 566 469 586 DNA Homo sapiens misc_feature (1)...(586) n = A,T,C or G 469acgcgggata ggtttggtcc tagcctttct attagctctt agtaagatta cacatgcaag 60catccccgtt ccagtgagtt caccctctaa atcaccacga tcaaaaggga caagcatcaa 120gcacgcagca atgcagctca aaacgcttag cctagccaca cccccacggg aaacagcagt 180gattaacctt tagcaataaa cgaaagttta actaagctat actaacccca gggttggtca 240atttcgtgcc agccaccgcg gtcacacgat taacccaagt caatagaagc cggcgtaaag 300agtgttttag atcaccccct ccccaataaa gctaaaactc acctgagttg taaaaaactc 360cagttgacac aaaatagact acgaaagtgg ctttaacata tctgaacaca caatagctaa 420gacccaaact gggattagat accccactat gcttagccct aaacctnaca gttaaatcaa 480caaaactgct cgccagacac tcgagccaca gcttaaaact caaggacctg cgggcttcat 540atccctctag angacctgtc tgtaatcgat aaccccgatc aacctn 586 470 487 DNA Homo sapiens misc_feature (1)...(487) n = A,T,C or G 470acggccaggg ctattggttg aatgagtagg ctgatggttt cgataataac tagtatgggg 60ataaggggtg taggtgtgcc ttgtggtaag aagtgggcta gggcattttt aatcttagag 120cgaaagccta taatcactgc gcccgctcat aaggggatgg ccatggctag gtttatagat 180agttgggtgg ttggtgtaaa tgagtgaggc aggagtccga ggaggttagt tgtggcaata 240aaaatgatta aggatactag tataagagat caggttcgtc ctttagtgtt gtgtatggtt 300atcatttgtt ttgaggttag tttgattagt cattgttggg tggtgattaa tcngttgntg 360atgaaatatt tggaggtggg gatcaatana gggggaaata gaatgatcag tacctcgccc 420gcgaccacgc taagggccaa tccacacact ggcggncgta ctaatggatc ccaactcgga 480ccagctt 487 471 488 DNA Homo sapiens misc_feature (1)...(488) n = A,T,C or G 471actgcggcgg gtaggcctag gattgtgggg gcaatgaatg aagcgaacag attttcgttc 60attttggttc tcagggtttg ttataatttt ttatttttat gggctttggt gagggaggta 120ggtggtagtt tgtgtttaat atttttagtt gggtgatgag gaatagtgta aggagtatgg 180gggtaattat ggtgggccat acggtagtat ttagttgggg cattcccgcg tacctatttg 240tatttttggt agagacaggg ttttgccatg ttggccagga tggtcttgaa ctactgacct 300caggtgatcc tcacgccttt atctcccaaa gtgctgcgat tacaggcatg aggcaccact 360cctggccaca ttcttatatt taaaaaaaaa gcacaactct attgtctact ggtgttcttt 420tacctgaagt tcaaactcta gctcttcaaa aaaaaaaaaa aaaaaaagta cctnggccgc 480naccacnc 488 472 586 DNA Homo sapiens misc_feature (1)...(586) n = A,T,C or G 472ggtacttgat gccctccaag caattaaaac caagggcaaa cgagccccat tcacaaattt 60tgacccctct actctccttc cttcatccct ggatttctgg acctaccctg gctctctgac 120tcatcctcct ctttatgaga gtgtaacttg gatcatctgt aaggagagca tcagtgtcag 180ctcagagcag ctggcacaat tcagcagcct tctatcaaat gttgaaggtg ataacgctgt 240ccccatgcag cacaacaacc gcccaaccca acctctgaag ggcagaacag tgagagcttc 300attttgatga ttctgagaag aaacttgtcc ttcctcaaga acacagccct gcttctgaca 360taatccagta aaataataat ttttaagaaa taaatttatt tcaatattag caaagacagc 420atgccttcaa atcaatctgt aaaactaaga aacttaaatt ttagttctta ctgcttaatc 480aaataataat tagtaagcta gcaaatagta atctgtaagc ataagcttat gcttaaatca 540gtttagtttg aggaatcttt aaaattacca ctaantgatt gnatgg 586 473 575 DNA Homo sapiens misc_feature (1)...(575) n = A,T,C or G 473ggtacaaagg ggaaagggtg catgccaact atcgaattat aggatatgta aaaaatataa 60gtcaagaaaa tgccccaggg cccgcacaca acggtcgaga gacaatatac cccaatggaa 120ccctgctgat ccagaacgtc acccacaatg acgcaggaat ctatacccta cacgttataa 180aagaaaatct tgtgaatgaa gaagtaacca gacaattcta cgtattctcg gagccaccca 240agccctccat caccagcaac aacttcaatc cggtggagaa caaagatatt gtggttttaa 300cctgtcaacc tgagactcag aacacaacct acctgtggtg ggtaaacaat cagagcctcc 360tggtcagtcc caggctgctg ctctccactg acaacaggac cctcgttcta ctcacgccca 420aagaatgaca taggacccta tgaatgtgaa atacagaacc cagtgggtgc cacccgcant 480gcccantcac cctgaatgtc cgtatgagtc aatcctgccg gcggccgttc naanggcgaa 540ttccacacac tggcggccgt ctaatggatc cactc 575 474 515 DNA Homo sapiens misc_feature (1)...(515) n = A,T,C or G 474ggtacgtggg ggactcaact gaaatcatgg cgtttgacag cacttggaag gtagaccgga 60gtgaaaacta tgacaagttc atggaaaaaa tgggtgttaa tatagtgaaa aggaagcttg 120cagctcatga caatttgaag ctgacaatta cacaagaagg aaataaattc acagtcaaag 180aatcaagcgc ttttcgaaac attgaagttg tttttgaact tggtgtcacc tttaattaca 240acctagcaga cggaactgaa ctcaggggga cctggagcct tgagggaaat aaacttattg 300gaaaattcaa acggacagac aatggaaacg aactgaatac tgtccgagaa attataggtg 360atgaactagt ccagacttat gtgtatgaag gagtagaagc caaaaggatc tttaaaaagg 420attgaccatt attcttggcg cacagtccaa aatncaaatt ggccagaaga tctatattgn 480acctgcccgg gcggccgttc gaaaggccaa ttcca 515 475 580 DNA Homo sapiens misc_feature (1)...(580) n = A,T,C or G 475acaaagatct gacatgtcac ccagggaccc atttcaccca ctgctctgtt tggccgccag 60tcttttgtct ctctcttcag caatggtgag gcggataccc tttcctcggg gaagagaaat 120ccatggtttg ttgcccttgc caataacaaa aatgttggaa agtcgagtgg caaagctgtt 180gccattggca tctttcacgt gaaccacgtc aaaagatcca gggtgcctct ctctgttggt 240gatcacacca attcttccta ggttagcacc tccagtcacc atacacaggt taccagtgtc 300gaacttgatg aaatcagtaa tcttgccagt ctctaaatca atctgaatgg tatcattcac 360cttgatgagg ggatcggggt agcggatggt gcgggcatca tgagtcacca gatgagggat 420tccttttgtg ccccaaagat ctttctnact ttgacaactt gaccttggnc gcgaccaccc 480taaggcgaat tcacccactg gcggccgtct aatggatccn nctcggncca acctggntat 540atggcntaan tnntccnggn naaatntntc cccncaatcc 580 476 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 476ggtactatgt gggacagtat tttgcaaata caagaagagc tcagggcagc tgtggagctg 60gatggtctgc ctggcaggcc tctgtgcagt ctgcctgctc atcctgtccc ctttttgggg 120cttgatcctc ttctcggtgt catgcttcct catgtatact tacttatctg gccaagaatt 180gttacctgtg gatcagaagg cagtcctggt gacaggtgtg attgcgggct tggccatgct 240ttgtgcaagt atctggatga gctgggcttc acggtatttg ccggagtttt gaatgaaaat 300ggcccaggag ctgaggaatt gcgaagaacc tgctctccgc gcctctcggt gctccaaatg 360gacatcacga accagtgcag ataaaagatg cttacagcaa ggttgcaaca atgctgcagg 420acaaaagact gtgggctgtg atcaacaatg ctngggtgct tggcttttcc actgatgggg 480agcttnttnt tatgatgact acnaacaatc ntggccgnga acttttttga actgngaggg 540acaaaacgtt tttccttttt taaaaaancc aagggnggtg gnnaattncn nnt 593 477 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 477actacaaggt ttagcatttg ctctgctggt cgacattccc ccagtctatg ggttgtatgc 60atcctttttc ccagccataa tctacctttt cttcggcact tccagacaca tatccgtggg 120tccgtttccg attctgagta tgatggtggg actagcagtt tcaggagcag tttcaaaagc 180agtcccagat cgcaatgcaa ctactttggg attgcctaac aactcgaata attcttcact 240actggatgac gagagggtga gggtggcggc ggcggcatca gtcacagtgc tttctggaat 300catccagttg gcttttggga ttctgcggat tggatttgta gtgatatacc tgtctgagtc 360cctcatcagt ggcttcacta ctgctgctgc tgttcatgtt tttggnttcc caactcaaat 420tcatttttca agtgacagtc ccgtcacaca ctgatncagt ttnaatttta aaagtacctc 480ggccgcganc accctaaggc gaatttnaac ccactngcgg ccgttctant ggatccaact 540ngnnncaaac ttngngaata ngggcataac ngntcctggg gaaatnnttc ccnct 595 478 420 DNA Homo sapiens misc_feature (1)...(420) n = A,T,C or G 478ggtacacagt atgtataaca atgcatacta tggtgtggag ttaattccaa ttaccatatt 60ttatatttat tggtcacaac agcatacatt ttatgctcca aaatacatgg atctgacaaa 120atggttacat ttaatgttct tttaaagaaa gatgaactaa atttaagaag aattggtttt 180tcctaatatc tcattttcaa attactgata caaatttgcc agagaaacaa ttacatgttt 240tacctaacat caaataatct ccagtttcta agacagatgc atttcttgtt caatttccaa 300aagtaaataa aggctttcta actgaaaaca tttgcatccc tagctctcta aagtaattaa 360aaagaaaatt acaaaaaatg acctctaagc ttctgaacag cccacttant tacataaagt 420 479 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,C or G 479ggtacctagt cagatggtag acgagctgtc tgctgccgca ggagcacctc tatacaggac 60ttagaagtag tatgttattc ctggttaagc aggcattgct ttgccctgga gcagctattt 120taagccatct cagattctgt ctaaaggggt tttttgggaa gacgttttct ttatcgccct 180gagaagatct accccaggga gaatctgaga catcttgcct acttttcttt attagctttc 240tcctcatcca tttcttttat acctttcctt tttggggagt tgttatgcca tgatttttgg 300tatttatgta aaaggattat tactaattct atttctctat gtttattcta gttaaggaaa 360tgttgagggc aagccaccaa attacctang ctgaggttag agagattggc cagcaaaaac 420tgtgggaaga tgaactttgt cattatgatt tcattatcac atgattatag aaggctgtct 480taatgcaaaa aacatactta catttnanac atattccaan gggatctcnc attttgtaaa 540aagttgacta ttactggagt aaaccctgtt ttccctaant ttaacttttt ttgggaaatt 600at 602 480 600 DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 480ggtacttttt tttttttttt tttttttttc ggtttgaggg ggaatgctgg anattgtaat 60gggtatggan acatgtcata taagtaatgc tagggtgagt ggtaggaagt tttttcatag 120gaggtgtatg agttggtcgt agcggaatcg ggggtatgct gttcgaattc ataaaaacag 180ggaggttana agtagggtct tggtgacaaa atatgttgtg taaagttcag ggganagtgc 240gtcatatgtt gttcctagga aaattgtagt ggtgagggtg tttattataa taatgtttgt 300gtattcggct atgaaaaata gggcgaaggg gcctgcggcg tattccatgt tgaagcctga 360gactagttcg gactcccctt cggcaaggtc caaaggggtt ccggttggtc tcttctagtg 420tggagataaa tcatattatg gccnagggtc atgatggcag gagtaatcaa aggggtcntt 480tgttttgaaa aagggnggan aggttaaagg ancccctttt tataatggtg atantaaaaa 540gatgcttggg ggactcnttt aaaatgttgg ctcttcttcc angcncccac aggcgtattt 600 481 594 DNA Homo sapiens misc_feature (1)...(594) n = A,T,C or G 481cgaggtacgg ccagggctat tggttgaatg agtaggctga tggtttcgat aataactagt 60atggggataa ggggtgtagg tgtgccttgt ggtaagaagt gggctagggc atttttaatc 120ttagagcgaa agcctataat cactgcgccc gctcataagg ggatggccat ggctaggttt 180atagatagtt gggtggttgg tgtaaatgag tgaggcagga gtccgaggag gttagttgtg 240gcaataaaaa tgattaagga tactagtata agagatcagg ttcgtccttt agtgttgngt 300atggttatca tttgttttga ggttagtttg attagtcatt gttgggtggt gattantccg 360ttgttgatga gatatttgga ggtggggatc aatagagggg gaaatagaat gatcagtacc 420tgcccnggcg gncgctcgaa anggcgaatt ccaccacact ggcgggcgnt ctaatggatn 480cgacccngtc ccaacttgcg taatcatggc atacttgttn ctggtgaaat ggtatccctc 540acaattccca cacatacaac ccgaacctaa atgtaaanct gggggcctat natn 594 482 600 DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 482accatgaaat acatatattt cataaggttc agttacaaaa tggattgttt caaatggcaa 60tttcttacac taacctgatt atgaaaaaaa gaagtctgta tcatctgctt ccaagtctgt 120tatgtccaaa tatattttaa ttatgcattt attttgctac ttttataaat attagagatt 180tcaccttaaa ttatttttgt aactagttct agaacatgtt ttccaattat tatttttcta 240atggagacat ataattgacc tatgtttatg catatatgtt ctctacacag tgaaactttt 300tttaaaaaga atagtaaaga aaatgcggaa gctctggctc tccaaggcaa agtcaaaaaa 360aaaaaaaaag cgggggggaa tgcgaggaac attttattac acctnctgat tttcctcctt 420gagntttatt ttctcccctt ggntatttgt taatgctaga aactgnattc ctaanaaagc 480atacctcttt caggngagcn tgataattgg gaanaatttt gttcctttag tntgaacatt 540ttattaagaa gngattccta ataaaganac aangggctnt ttaattnttt gggggnngga 600 483 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 483acagaacatc gtcagcacta gcacagttta cagaacctca cagacccaaa ggaacatcaa 60taggcaaagc gactacagga ggcgtgtgtc cgcgtgggcg aggtaaagag ggtcagtatt 120ggtcaagtga cagtgtcggt aatctggcaa gacagtgatg ttaagaaggt tcatagttta 180agaattatct aaaatatttt aaaaactata aagctgcaac acatgatttt tacacctagt 240tactagaaaa ctaaggaaag cacttattag ctctgaataa agtaacatgg aaagcacttt 300tactaatcga caaaaaaacc ttctaatgca ttatcagaaa gattttataa tacaaggagg 360catattgctc agtcagaagg ggttctataa gaaaagcact tactaagtta gcgactaaca 420gaacaaccng tttaaagatg aattaaatgc cccatttggg gangcatggc aggtgttaag 480anaaangaaa agcntaagaa aacatttnct ggttatanca aacctttntt tnttatctac 540tgnatttgac aaaaattaac cntttaaagt tttacccngg cacttnnttc nttgtcctcg 600gcccg 605 484 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 484ggtacgcggg tggggagacc ctggggtagc agccactgac ctcacacctg gaggaagctg 60tgtgaccgat tcatgagctt atgcctgaag acagagcaag cactccccgc accacgacga 120tgacgttcac ttgttttgtg tttttcgatc tcttcaacgc cttgacctgc cgctctcaga 180ccaagctgat atttgagatc ggctttctca ggaaccacat gttcctctac tccgtcctgg 240ggtccatcct ggggcagctg gcggtcattt acatcccccc gctgcagagg gtcttccaga 300cggagaacct gggagcgctt gatttgctgt ttttaactgg attggcctca tccgtcttca 360ttttgtcaga gctcctcaaa ctatgtgaaa aatactgttg cagccccaaa gagagtccag 420atgcaccctg aaagatgtgt agtggaccgc acttccgcgg naccttccta atnatttcaa 480ctgggtgnga ctgtggccct gccctgtttc ttcttagggg agactttang anggcgagcn 540tcataccgga tagttttctt taggaaactn aggaaccttg gctcaggacc a 591 485 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 485ggtacgcggg gatataaagg gagagagcaa gcagcgagtc ttgaagctct gttnggtgct 60tnggatccat ttccatcggn ccttacagcc gctcgtcaga ctccancagc caanatggtg 120aancagatcg agagcaagac tgcttttcan gaagccttgg acgctgcang tgataaactt 180gtagnagttg acttctcagc cacgtggtgt gggccttgca aaatgatcaa gcctttcttt 240cattccctct ctgaaaagta ttccaacgtg atattccttg aagtagatgt ggatgactgt 300caggatgttg cttcagagtg tgaagtcaaa tgcatgccaa cattccagtt ttttaagaag 360ggacaaaagg tgggtgaatt ttctggagcc aataaggaaa agctttgnag ccnccattaa 420tgaatgagtc taatcatgtt ttctgaaaac ataacccagc catttggcta tttaaaactt 480gnaanttttt nagntaccna aatttaaagt ctgaagacat aacccggtgc catttgcgtg 540acaatnaaaa attatgccaa cactttttna anaanganan nnntttcctn gggaaatngt 600anccc 605 486 319 DNA Homo sapiens 486ggtaccagtt gtagccataa agattctggg actcattatg gactactaga aggacctcct 60tcccttctgc gacattgaac ggcacgacat caatattggt ctgggcactg tttggcaggt 120tccagaaggt taaaagcgag gctgtgagca ggagtccctg ccagggaatg cacactctgt 180atggacaggc tgaaggggac cccatggtct ctgctgcctg cttgtcctct gtggagaaga 240gcttgggctc caggaactct cttgtcaggg ctgctgtgac tgtcagctct gctgtccttc 300ctacctctgt gtcccgcgt 319 487 586 DNA Homo sapiens misc_feature (1)...(586) n = A,T,C or G 487acgcgggagc tgagtgtccc gcggggcccg aagcgtttac tttgaaaaaa ttagagtgtt 60caaagcaggc ccgagccgcc tggataccgc agctaggaat aatggaatag gaccgcggtt 120ctattttgtt ggttttcgga actgaggcca tgattaagag ggacggccgg gggcattcgt 180attgcgccgc tagaggttaa attcttggac cggcgcaaga cggaccanag cgaaagcatt 240tgccaagaat gttttcatta atcaagaacg aaagtcggag gttcgaagac gatcagatac 300cgtcgtagtt ccgaccataa acgatgccga ccggcgatgc ggcggcgtta ttccatgacc 360cgccgggcag ctttcnggaa accaaagtct ttgggttncc gggggagtat ngttcnaaaa 420aaaaaaaaaa aaaaaaaagt cctnggccgg gancccctta nggngaaatt cagccactgg 480nggcgttctn atggatncna gctcggncca acntggcgta atatggcata cttgttcctg 540gngnaaatgt ttccctccaa attccccaaa tacgggcgga gcttaa 586 488 487 DNA Homo sapiens misc_feature (1)...(487) n = A,T,C or G 488acagctggtt ggacctattc atgcatcttc accagcagct ggagcatctc cacccttggt 60atttctggtg taaattactt gagctctgtg ctttgaaacc agtttgataa gtcctttact 120aaggagctcc tgaagggctg ccctggccag ggagcctcga atcttcagtc tctcagagac 180cacagctggg gttataagtt tatagttggg aacttcctta cagagtttat cataggtagc 240tttgtcaaac aagactaagt tattgagctt gtcccgaact ttgcctttgg accacttctt 300ctttttggcc ttgcccccgg atttgttcac tgggtctttg tctttcttgg ccgactttcc 360agcgtccttc ttcttcttgt cgtccttaag cggcattgcg aanctcggag aataagcaac 420aaacaccgca cctcgtcnaa gatgtcggac aaaaaaaggc cccgcgtacc ttnggccgcg 480ancacnc 487 489 589 DNA Homo sapiens misc_feature (1)...(589) n = A,T,C or G 489acgcggggtc tctcctcagg cagcagcaac gcggaggaaa cgggagtgaa cggagagcgt 60agtgaccatc atgagcctcc tcaacaagcc caagagtgag atgaccccag aggagctgca 120gaagcgagag gaggaggaat ttaacaccgg tccactctct gtgctcacac agtcagtcaa 180gaacaatacc caagtgctca tcaactgccg caacaataag aaactcctgg gccgcgtgaa 240ggccttcgat aggcactgca acatggtgct ggagaacgtg aaggagatgt ggactgaggt 300acaaagatta aattaagaca cggtaaattg actaaatatt tggtttttat ataaataaag 360gtcataacca caccgttgac atgtaatact gttataatac aacagttaaa ctttgtgagt 420ctcaacagaa gtcatctgta gttnaacagg aaacaaaagt tgaaaaaaaa catgttnaaa 480caaaactctg ggactaacag gtcgggattg taagtacaac caacatattc ctcacttctg 540ggtntttcaa gtttacagta cttggccgga cccccttang ggnattcac 589 490 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 490ggtaccggga tagtttttgc agggttttat tttataaaat ccaagcgcgc tgttgattgt 60gttttccttg ttttcagccc cccgactcca gcccgcagca catttccgct gtccgtcagt 120aattgtgtcc tctctttatg cttgcttggg gaatgttgtt ttctgactag gctgatcatt 180atctaaagaa tctaattctg ttgattttta aaacttttag gaccataaac gttgtgttca 240tatatggaca tggaaatatt tatataattt tatagaaaat aaccttttag atggtcaaag 300tgtaaggagt tttttttgtc agataatcat ttctacttca aaaacatttc atgcaatatt 360agaataaagt tcctgtcatt cctctnnnan aaaaannnnn nnnnnnanna nnnnnnnnnn 420nggaanannn nnnnnnnnnn aaaaaagtac ctgcccnggc ggccgttcaa aaggcgaatt 480ccacccactg gcggccgttc taatggatcc anctcggacc aacctggnga aacatggcat 540acctgttcct ggngaaatgg tntcccttac aattcccaca aataaaaccg g 591 491 583 DNA Homo sapiens misc_feature (1)...(583) n = A,T,C or G 491ggtacatata aatccttttg gtgtcacttg tagcaagcct tgcttctgca gttttcggat 60tttcctcaaa gctttgttgc gcttgcgtag aattcgaagt ggactaaagc caacagcatc 120gataagtttc cgcctaaaga aaccaatgtt tgcaaagtag ataggagatg gacatctgaa 180aattttcact ccttctggct catacatatc ataataatct tttttattct tatagatgtt 240ggttcttcca atattagcca gcgtgctgca ttttggaaat tgggtcctga acacgatggt 300tagcagttga aatgccacac tagctgccag gcctaacccg agtcccagga caatggtgaa 360agatgaaagg catgaaccca aataaacaat catatttggn cnttccccca atctgctatt 420ttaaccaact gcatcaacat tcctttaagt tccaatgcta aactggcang acnggcnttt 480gtagaagngc cangaaaaat cagngcttga cgacaatcac accatgatgn nccataancc 540acaatctggg nttggctcnn ggcctctgaa cnnngactgg nag 583 492 597 DNA Homo sapiens misc_feature (1)...(597) n = A,T,C or G 492acgcgggggg tggcacggag gaaccaggag cgtgccctgc gcaccgtctg gagctccgga 60gatgacaagg agcagctggt gaagaacaca tatgtcctgt gaccgccctg tcgccaagag 120gactggggaa gggaggggag actatgtgtg agcttttttt aaatagaggg attgactcgg 180atttgagtga tcattagggc tgaggtctgt ttctctggga ggtaggacgg ctgcttcctg 240gtctggcang gatgggtttg ctttggaaat cctctangag gctcctcctc gcatggcctg 300cagnctggca acaaccccga gttgtttcct cgctgatcga tttctttcct ncaggtagag 360ttttctttgc ttatgttgaa ttccattgcc tttttctcat cacaaaaaat gatgttggga 420atcgnntctt ttgtttggct gaattatggg ntttttaant ataaaccaaa nttttttatt 480aacattctta aanaagggaa agtnnaatgt ncnttggncc cnaccncgct aanggcnaat 540ttcancccnt ggnggccgtn nttnnggatc cnnncnngnn ccaannntgg nntantn 597 493 591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 493acggatgcta cttgtccaat gatggtaaaa gggtagctta ctggttgtcc tccgattcag 60gttagaatga ggaggtctgc ggctaggagt caataaagtg attggcttag tgggcgaaat 120attatgcttt gttgtttgga tatatggagg atggggatta ttgctaggat gaggatggat 180agtaataggg caaggacgcc tcctagtttg ttagggacgg atcggagaat tgtgtaggcg 240aataggaaat atcattcggg cttgatgtgg ggaggggtgt ttaaggggtt ggctagggta 300taattgtctg ggtcgcctag gaggtctggt gagaatagtg ttaatgtcat taaggagaga 360aggaagagaa gtaagccgag ggcgtctttg attgtgtagt aagggtggaa ggtgatttta 420tcggaatggg aggtgattcc taagggggtg gttgatcccg tttcctgcca agaataagaa 480gtggaatgct gctagggctg cattaatgaa ggccaagatg aaatgaaagg taaanaatcn 540ngtgangggg gactgctact gatancctct caaatcatga ataggntgtc c 591 494 374 DNA Homo sapiens misc_feature (1)...(374) n = A,T,C or G 494ggtacttttt tttttttttt tttttttttt ttttttagnt catgtctttt attaactcat 60acagttactt gtcttctggt ttgttgaaac agtaagtcan acaacatttg ccacaataat 120gtctgtcaaa gtgacttgcc ataaacaccc cagcaccaca ttcatcanaa gggcactctc 180gacgaaggcg actaattttg ccattctcat ccaccttata atatttcagg acagccagct 240taaccttctt tctcttgtgc ttattcttct tgggagnggt gtaagacttc ttcttccttt 300tcttagcacc accacgaagt ctcaacacaa gatgaagagt agactccttt tgaatattgt 360aagtcagaca aagt 374 495 597 DNA Homo sapiens misc_feature (1)...(597) n = A,T,C or G 495actgggagaa ggtgctgacg ccgacgaagt ggtggatggg cttcccgctg caggtgaacc 60tcctggtgcc atcctgcagg gtcccccgag gattgcctag atcatttttc aagcagtagt 120tgctttctgg gtttttacaa attctgcatt ttccacactg aggagtaaag agcgggatga 180ctttatcacc tggtttgact gtagtcaccc cttctccaac actttccacg atgccggctg 240cctcatggcc taaaatcaca ggaagggggg tcaccaggtt gccactaacc acatgctcat 300ctgaacgaca gattcctgca gccaccatct taatgcgaac ttcatgagcc ttaggaggtg 360caacctctac ctcctcaatg gaaaagggtt tctttaactc ccatagcaca actgctttgc 420atttgattac ctgtaaactc agctacttgt gaaggctgag gcanganaat actttgaacc 480ccggaaggca aaggttgcaa tgagccnana acaccattgn acttccanct gggcaatana 540aaaaaactca tttttcctgc tggctcaaat gatctgcttc ttgcaaacaa gagntgn 597 496 604 DNA Homo sapiens misc_feature (1)...(604) n = A,T,C or G 496ggacgcgggt gctgactgca tagctctttt tcttgagagg ctctccattt tgattcagaa 60agttagcata tttattacca atgaatttga aaccagggct tttttttttt tttgggtgat 120gtaaaaccaa ctccctgcca ccaaaataat taaaatagtc acatttatct ttattaggta 180atcacttctt aattatatgt tcatactcta agtatcaaaa tcttccaatt atcatgctca 240cctgaaagag gtatgctctc ttaggaatac agtttctagc attaaacaaa taaacaaggg 300gagaaaataa aactcaagga gtgaaaatca ggaggtgtaa taaaatgttc ctcgcattcc 360cccccgcttt tttttttttt ttgactttgc cttggaaagc cagagcttcc cgcattttct 420ttactattct ttttaaaaaa agtttcactg ngtaaaagaa catatttgcc taaacatang 480tcaattatat gtctccatta naaaaaaata attggnaaac attgtctana actagttcca 540aaataattaa gggggaaatc tntaatnttt ttaaagtgcc naaanaatgc ctaanttaaa 600antt 604 497 587 DNA Homo sapiens misc_feature (1)...(587) n = A,T,C or G 497acattaatga aatgtttcca aagaaatact gaacaatata tactctagtt tgctgaggtt 60ccagctcgag ttcaaaccta attcttgtgc aataaaaatc agcatggatc ttagatgatc 120tagaatacac tgtgttttga aatccacagc tggtttcatt tttaaccatt atgaaaaacc 180agtacttttt tttttttttt tttttttttc nctnggacca taaattttta ttggcaggtc 240aggaaaaaag ccgggggtaa gggtcccttc cttcccatcc ctctacccan aanacaccct 300ccaaaggaca gcagaagccc cagagcctgc tgcctcagag gaccttggag gcagacaaat 360tgttgtagng atcttcctgt ccctcaanca ggctgcggta ggtggnaatc tnctgctcca 420gccgcgactt gatgtccatg aaccgctggt cctcggccgc gacaccctta nggcgaattn 480caccnactgg gnggcgttct agtggatccg actcggacca acctngcgna atcatggcan 540actggttnct gnnggaaatg gtttccctnc aattccccaa cataccn 587 498 354 DNA Homo sapiens 498acgcgggcaa taaagctaaa actcacctga gttgtaaaaa actccagttg acacaaaata 60gactacgaaa gtggctttaa catatctgaa cacacaatag ctaagaccca aactgggatt 120agatacccca ctatgcttag ccctaaacct caacagttaa atcaacaaaa ctgctcgcca 180gaacactacg agccacagct taaaactcaa aggacctggc ggtgcttcat atccctctag 240aggagcctgt tctgtaatcg ataaaccccg atcaacctca ccacctcttg ctcagcctat 300ataccgccat cttcagcaaa ccctgatgaa ggctacaaag taagcgcaag tacc 354 499 632 DNA Homo sapiens misc_feature (1)...(632) n = A,T,C or G 499nccgaggtac caactgcact cgttttggca ttgcagctaa atatcagttg gatcccactg 60cttccatttc tgcaaaagtc aacaactcta gcttaattgg agtaggctat actcagactc 120tgaggcctgg tgtgaagctt acactctctg ctctggtaga tgggaagagc attaatgctg 180gaggccacaa ggntgggctc gccctggagt tggaggctta atccanctga aaagaaacct 240ttgggaatgg atatcaaaag aattggcctt aatatatttc cattgngacc agcagcaggc 300tttttttccc ccagaagatg atcaaaacaa aaggatgatc tcaacaagaa ctgtatttta 360aagtatttaa ganagtcttt ggtaactngg ttctaagtng gtatctaatt acccaatgct 420gcagtcctgc agtccctatt cattanttaa atgtatttaa ctggtaaatg ccctncccnc 480cataatgaaa taganccttt ttgaaaaccc aaaaaaaaaa aaaaaaaaaa aaaaaagtcc 540ctgcccggcc ggccctcaaa nggngaattc cannccctgg gggccgtact aanggatccn 600cccggnccaa cttggggaat atgggntant gn 632 500 619 DNA Homo sapiens misc_feature (1)...(619) n = A,T,C or G 500tccagcggnc cgccgggcng gtcatctata aaaggaaaag tgatggcatc tatatcataa 60atctcaagan gacctgggag aagcttctgc tggcagctcg tgcaattgtt gccattgaaa 120accctgctga tgtcagtgtt atatcctcca ngaatactgg ccaaanggct gtgctgaant 180ttgctgctgc actggaacca ctccaattgc tggccgcttc actcctggaa ccttcactaa 240ccagatcagg caaccttccg ggaccacggn ttnttgtggt tactgacccc aaggctgacc 300accaacctnt cacggaggca ttttatgtta acctacctac cattgcgctg tgtaacacaa 360gattcttctc tgcctatgtg gacattggca ttccatgcaa caaccaaggg gagctcactc 420aatgggtttg atgtggtgga tctgctcggg naagtctgcg catgcctggc accatttccg 480tgaacaccat ggagggatgc ctgattttac cttggccgga cacnctangg cgaattcacc 540acttggngcc gtatantgga tccactcgga ccaacttggg naaaatggca naatnttccg 600gggaaatgat ccctccaan 619 501 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 501accacactga gatagtgttt gccaggacct cccctcagca gaagctcatc attgtggaag 60gctgccaaag acagggtgct atcgtggctg tgactggtga cggtgtgaat gactctccag 120ctttgaagaa agcaaacatt ggggttgcta tggggattgc tggctcagat gtgtccaagc 180aagctgctga catgattctt ctggatgaca actttgcctc aattgtgact ggagtagagg 240aaggtcgtct gatctttgat aacttgaaaa aatccattgc ttatacctta accagtaaca 300ttccccgaga tcaccccgtt cctgatattt attattgcaa acattccact accactgggg 360actgtcacca tcctctgcat tgacttgggc actgacatgg gtnctgccat ctcctggctt 420atgagcaggc tggagggcat catgaanaaa cagcccaaaa tccaaacaga caacttgtga 480atgancnggt gatcacatgg ctatggcaga atggatgatc nagncctggg aggttcttac 540ttacttggaa tctgntgaaa cggttcttcc aatacctntt ggcctccatg gntggaanac 600cctga 605 502 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C or G 502acatcttgct ggaaaatgct gcccagggct ctggagacgg tggctgcccg ggctcccttc 60actgtccagg tcctgaaaga ctcttgttca tgaactgtct cttcacaaag caagtccacc 120acttgctggg tttatcattc tgagggtcga aaactttctc acaaagtctc agtccagtct 180cttgccttag ctgttgtaaa taggctctca tcacttcatc ttctgtttgt ttgcaggttt 240ggcataaatt gcgttaagtg gaaaaccagg ctctccagga atgggaaaat taagtgattc 300ccagcgtata catttctttc tcaccttggc ttttggaatt gcacttttgc agtttcttca 360nacattcaga aatgtagaga gttatatata tcaangncct atcaacttca ttcttaattt 420cataagtttt gaaaaaaaca ttggcccttg aagtaataaa tngntttatt cccaaaatct 480ggatcntttg gcnctctngg ggcangnccc ttgaaatgac ttttgatagg gaacaangcc 540ctggtttcca nnagnttggg ttcnggaccn taaaaaaaaa gggaanccgg nttttggngg 600gcccggttta acccaagggc cggancn 627 503 629 DNA Homo sapiens misc_feature (1)...(629) n = A,T,C or G 503ggtacattag tagagctctc caatcacagg cagacgccag tgtcctatga ccagggggca 60aatatggcca aacagattgg agcagctact tatatcgaat gctcagcttt acagtcggaa 120aatagcgtca gagacatttt tcacgttgcc accttggcat gtgtaaataa gacaaataaa 180aacgttaagc ggaacaaatc acagagagcc acaaagcgga tttcacacat gcctagcaga 240ccagaactct cggcagttgc tacggactta cgaaaggaca aagcgaagag ctgcactgtg 300atgtgaatct ttcattatct ttaatgaaga caaaggaatc tagtgtaaaa aacaacagca 360aacaaaaagg tgaagtctaa atgaagtgca cagccaaagt catgtatcca gaggcttang 420aggcgtttga gangatactc atctttttgg aatnctgcct taggttcggc atgtanacca 480agtgatgaga agtgaatcca tggaagagtt ttaatgtgac ttggaaaata tgccaaaaaa 540tgagagatcc aataacttna ggaaaataag ggggatccaa tncctncccg gcggccctta 600ggggaattca aacactnggg gcggtatan 629 504 462 DNA Homo sapiens misc_feature (1)...(462) n = A,T,C or G 504acgcgggagc tgagtgtccc gcggggcccg aagcgtttac tttgaaaaaa ttagagtgtt 60caaagcaggc ccgagccgcc tggataccgc agctaggaat aatggaatag gaccgcggtt 120ctattttgtt ggttttcgga actgaggcca tgattaagag ggacggccgg gggcattcgt 180attgcgccgc tagaggttaa attcttggac cggcgcaaga cggaccagag cgaaagcatt 240tgccaagaat gttttcatta atcaagaacg aaagtcggag gttcgaagac gatcagatac 300cgtcgtagtt ccgaccataa acgatgcccg accggcgatg cggcggcgtt attccatgac 360ccgncgggca gcttccggga aaccaaagtc tttgggttcc ngggggagta tnggtgcaaa 420aaaaaaaaaa aaaaaaaaaa gtcctnggnc gcgaccccct aa 462 505 628 DNA Homo sapiens misc_feature (1)...(628) n = A,T,C or G 505actttttttt tttttttttt tttgggggag gttatatggg tttaatagtt tttttaattt 60atttaggggg aatgatggtt gtctttggat atactacagc gatggctatt gaggagtatc 120ctgaggcatg ggggtcaggg gttgaggtct tggtgagtgt tttagtgggg ttagcgatgg 180aggtaggatt ggtgctgtgg gtgaaagant atgatggggt ggtggttgtg gtaaacttta 240atagtgtagg aagctgaata atttatgaag gagaggggtc agggttgatt cgggaggatc 300ctattggtgc gggggctttg tatgattatg ggcgttgatt agtantaatt actggttgaa 360cattgtttgt tggtgtatat attgnaattg agattgctcg ggggaatang ttatgtgatt 420aggaataggg ttangatgag tgggaagaaa aaaagaaagg aantaaaagt ttaattattc 480cctttttggg ttgaagngat natggaaggg gaaaatttgg gccttgaaat tgtttaagta 540atacttttct aataaggtaa gtctagaaga atagggcngg ttttggtctt aaaaaggcta 600aaaggggatt ggcggggtgg atccnccc 628 506 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 506acggtagaac tgctattatt catcctatgt gggtaattga ggagtatgct aagattttgc 60cgtagctggg tttggtttaa tccacctcaa ctgcctgcta tgatggataa gattgagaga 120gtggggagaa ggcttacgtt cagtgaggga gagatttggt atatgattga gatgggggct 180agtttttgtc atgtgagaag aagcaggccg gatgtcagag gggtgccttg ggtaacctct 240gggactcaga agtgaaaggg ggctattcct agttttattg ctatagccat tatgattatt 300aatgatgagt attgattggt agtattggtt atggttcatt gccggagaag tatattgttg 360aagaggatag ctattagaag gattatggat gccgttgctt gcctgaagaa atacttgatg 420gcagcttctg tggaaccaag gtttattttt ttggntagaa ctggaataaa acctacatgt 480ttatttctan gccactcagg taaaaaatca tgcnaactta acccttgata atgtgcctcc 540aaaatgtaaa aaaataacgg ttggcccggg ataatcccgt ncttggccga ccccctaggn 600aattcccccc tg 612 507 632 DNA Homo sapiens misc_feature (1)...(632) n = A,T,C or G 507ggtactacgt tgtagcccac ttccactatg tcctatcaat aggagctgta tttgccatca 60taggaggctt cattcactga tttcccctat tctcaggcta caccctagac caaacctacg 120ccaaaatcca tttcactatc atattcatcg gcgtaaatct aactttcttc ccacaacact 180ttctcggcct atccggaatg ccccgacgtt actcggacta ccccgatgca tacaccacat 240gaaacatcct atcatctgta ggctcattca tttctctaac agcagtaata ttaataattt 300tcatgatttg agaagccttc gcttcgaagc gaaaagtcct aatagtagaa gaaccctcca 360taaacctgga gtgactatat ggatgccccc caccctacca cacattcgaa gaacccgtat 420acataaaatc tagacaaaaa aggaaggaat cgaacccccc aaactgggtt nagccaaccc 480catgggcttc acgacttttt tataaaaaaa aaaaaaaaaa aaaagtcctg gcccgggngg 540cggtcanggn gaaattcaac nactgggngg cggtctaang ggtccaactc gggnccaacc 600tgggggaaaa tgggaaagtg gttcctgggg aa 632 508 336 DNA Homo sapiens misc_feature (1)...(336) n = A,T,C or G 508cggtcctcta atgctgctcn cccggccgca ntgtgattgg atatcttgca gaattcgccc 60ttagcgtggt cgccgggccg aggtacaact tccaaaaagg agacattgga gaanaaccaa 120gctgggtcta taaggaattg cacatgagat ggcacacata tttatgctgt ctgaaggnca 180cgatcatgtt accatatcaa gctgaaaatg tcaccactat ctggagattt cgaccgtgtt 240ttcctctctg aatctgttat gaacacnttg gttggctgga ttcantaata aatatgtaag 300gcctttcttt tcaaaaaaaa aaaaaaaaaa aaaagt 336 509 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,C or G 509ggtacttttt tttttttttt ttttttttta tagatacaat tggcttttat ttgtgattca 60tgagtcaggg cagtttccat tctgcaaaat atagtgatag ctcctactgg gcaatacaac 120agtanaacag tgggttttgt aaaatgggaa tccaggaaca gaagaatata aataaattga 180tttaaataaa ctgattggtt aatttcagaa tacttcatat tacttttttc taagagttaa 240agcagaaagg actttcttac tgtgctgact canacagcct ggactctcat gtttttagga 300aaattttgct gttctgggat ctacctgctt cctcatgttt cagtgngagt atatggcatt 360taacatgact ggctccattc tggagtccca ggctgtccct aaatgagaag ttgactaaac 420ataaggnatt aacactactg ncaggtacca tcattttggc ttncatcatt catanggtat 480gatgncccnc naatcatacc tttatttgag tttttgncat tccnncccaa aaaaaaaatt 540ttgaanttta ccaaaggntg catgccacnt ttaaagggtt anaaaatcnc cccnccnggn 600actaatnttg ggccatcngn nggc 624 510 619 DNA Homo sapiens misc_feature (1)...(619) n = A,T,C or G 510acggatgcta cttgtccaat gatggtaaaa gggtagctta ctggttgtcc tccgattcag 60gttagaatga ggaggtctgc ggctaggagt caataaagtg attggcttag tgggcgaaat 120attatgcttt gttgtttgga tatatggagg atggggatta ttgctaggat gaggatggat 180agtaataggg caaggacgcc tcctagtttg ttagggacgg atcggagaat tgtgtaggcc 240aataggaaat atcattcggg cttgatgtgg ggaggggtgt ttaangggtt ggctagggta 300taattgtctg ggtcccctaa gaggtctggt gagaatagtg ttaatgtcat taangagaga 360aagaaaaaaa ataagcccga gggcgtcttt gattgtgtan taaaggtgga angtgatttt 420atcngaatgg gaagtgattn ctaaggggtt ggtttgatcc ctttcgtgcc aaaataagaa 480gnggaatgct gctagggctc cataatgaag gcaanataaa atgaaagnaa aaaatctgta 540aggnnggact gctactaata ncctcccaaa tcttgaacaa gntttnccaa ttntggatgg 600nggtataant tnaattcnn 619 511 634 DNA Homo sapiens misc_feature (1)...(634) n = A,T,C or G 511cgaggacgcg gggagatggc ctagaagcaa tgatagccat cactgagaac acctagcacc 60caatcttggt tcctaatacc attctcccat caaaggaacc agagatcctt ggagaaatgg 120ttaaggaatg aggcaggaaa tatacaagat aagcctggag catcttatag ctctagaaag 180taagaaagta cctgcctatt ttagaatcct agagaacatt tcattgtaag aaactagccc 240attatttaag tgtccacagt atttttcatt tcagtggtcc aagatgcgaa ggtttccaga 300cacaatcttg ttctctaata ctgctccagg tgggatatca attctgtccc catgatttgc 360aatgatgata cccgttccct ttaatgaaac attttttnca aatgtcacat cttctgaaac 420tgngaggnga tccaattcaa gcatatctgg gntactttcc aaatcntctt agataatctt 480gaaccttcgt aaaagaactg gctaattaan ccanggccct gnaggaaatt ccccttttcc 540tcattggcag anancctgca ttaaantntt aagggttgnn ttnccnccan aaactgtgtg 600gtttgnaggc aaaaaacggt cttgggcatt ancc 634 512 623 DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G 512ggtacgcggg cattgttcat gactttaaca agaaacttac agcctattta gatcttaacc 60tggataagtg ctatgtgatc cctctgaaca cttccattgt tatgccaccc agaaacctac 120tggagttact tattaacatc aaggctggaa cctatttgcc tcagtcctat ctgattcatg 180agcacatggt tattactgat cgcattgaaa acattgatca cctgggtttc tttatttatc 240gactgtgtca tgacaaggaa acttacaaac tgcaacgcag agaaactatt aaaggtattc 300agaaacgtga agccagcaat tgtttcgcaa ttcggcattt tgaaaacaaa tttgccgtgg 360aaactttaat ttgtcttgaa cagtcaagaa aaacattatt gaggaaaatt aatatcacag 420catacccccc cctttacatt ttgngcagng gatatttttt aaagcttctt tnatgtaagt 480agcaacangg ntttactatc tttcatttca taaatcaatt aaancnttnc ctcaaaaaaa 540aaaaaaaaaa aaaaatacct ncccggcggc gctccaaagg ggaattcaan caccggnggc 600cgtctttggg accaacncgg gcc 623 513 623 DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G 513actgccctct ccagatcagc agttcaggag agcacaggag gcaaaacaca gattgctggg 60cttattggtg ccatcatcgt gctgattgtc gttctagcca ttggatttct cctggcacct 120ctacaaaagt ccgtcctggc agctttagca ttgggaaact taaagggaat gctgatgcag 180tttgctgaaa taggcagatt gtggcgaaag gacaaatatg attgtttaat ttggatcatg 240accttcatct tcaccattgt cctgggactc gggttaggcc tggcagctag tgtggcattt 300caactgctaa ccatcgtgtt caggacccaa tttccaaaat gcagcacgct ggctaatatt 360ggaagaacca acatctataa gaataaaaaa gattattatg atatgtatga gccagaagga 420gtgaaaattt cagatgtcca tcttctatct actttgcnaa cattggnttc tttaggcngg 480aacttatcga tgctggtngg ctttagtnca ctttgnaatt tacgcaagcc ccacaaactt 540tgaggaaatc ccaaactgcn aancangntt nttcagtggt acccaanggt tttttttcct 600tggcccgacn ccctangnga atn 623 514 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C or G 514ggtactcatg cccgactgtc taccaggcac acagactttg aggagagggc gtatgtcgtc 60ttgatccgca tcaatgatgg gggtcggcca cccttggaag gcattgtttc tttaccagtt 120acattctgca gttgtgtgga aggaagttgt ttccggccag caggtcacca gactgggata 180cccactgtgg gcatggcagt tggtatactg ctgaccaccc ttctggtgat tggtataatt 240ttagcagttg tgtttatccg cataaagaag gataaaggca aagataatgt tgaaagtgct 300caagcatctg aagtcaaacc tctgagaagc tgaatttgaa aaggaatgtt tgaatttata 360tagcaagtgc tatttcagca acaaccatct catcctatta cttttcatct aacgtgcatt 420ataatttttt aaacagatat tccctcttgt cctttaatat ttgctaaata tttctttttt 480gangnggagt cttgctctgt cgnccaagct ggantacctg ncccggccgg ccgtcaaagg 540cgaattcaac aactggcggc cgtactaatg gatcgacctc ggaccaactt ggggaacatg 600gcanactngt tcctgngnaa aggatcc 627 515 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 515accattggtg gccaattgat ttgatggtaa gggagggatc gttgacctcg tctgttatgt 60aaaggatgcc gtanggatgg gagggcgatg aggactagga tgatggcggg caggatagtt 120cagacggttt ctatttcctg agcgtctgag atgttagtat tagttaagtt ttgttgtgag 180tgttaggaaa agggcataca ggactaggaa gcagataagg aaaatgatta tgagggccgt 240gatcatgaaa ggtgataagc tcttctatga taggggaaag taancgtctt gtanacctac 300ttgcgctgca tgtgccatcc cgccgtaccc taacccgtgc aaaggtagca taatcacttg 360ttccttaatt aagggacctg tatgaatggc ttcaccaggg ttcaactgtc tcttactttt 420aaccagtgaa attgacctgc ccctgaanag gcggcnttac acaccagacg agaaaacctt 480tggagcttaa ttattatcca acatacctng ccggaccccc taaggcgaat tccaccactt 540gcggcgtcta tggatccact cggaccactt ggggaaaagg ctactgtcct ggnaatgttt 600cctcn 605 516 464 DNA Homo sapiens misc_feature (1)...(464) n = A,T,C or G 516ggtacaacta atccgtgaca aattaccaga ttaattttac tttatttctt caggcctggg 60gtttttcgat gagttcaaat ttgggatctt caaatttgaa ggtgggaaat gtattcatgt 120ctgcattacc aaacatttgc ttgagcttaa aaagctccct ctccagctct tgctgatact 180ctgaactagc atcaacaggt cctccagatg tctgtcgctt agatttgtat tctctaatct 240tgtccacaaa gagtttctgt ataggatcaa gttccttatt aaatgccact gctgtaacac 300caatgttcct ccgcaaatgg actgagacgg ctgaccgaat gacagaggag aacctgaaga 360gcctctgaag aatcatgctg attcttgcac tcagtcccga gctgncaaag ccttcgccgc 420caccaccttc gntctacccc cgcgtacctg cccggcgggc gctc 464 517 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 517acccggagca cggagatctc gccggcttta cgttcacctc ggtgtctgca gcaccctccg 60cttcctctcc taggcgacga gacccagtgg ctagaagttc accatgtcta ttctcaagat 120ccatgccagg gagatctttg actctcgcag gaatcccact gttgaggttg atctcttcac 180ctcaaaaggt ctcttcagag ctgctgtgcc cagtggtgct tcaactggta tctatgaggc 240cctagagctc cgggacaatg ataagactcg ctatatgggg aagggtgtct caaaggctgt 300tgagcacatc aataaaacta ttgcgcctgc cctggttagc aagaaactga acgtcacaga 360acaagagaag attgacaaac tgatgatcga gatggatgga acagaaaata aatctaagtt 420tggtgcgaac gccattctgg gggtgtcctt tgccgtctgc naaactggtg ccgttgagaa 480gggggtcccc tgtccttggc cggacacnct aaggcgaatt ccacacactg cggccgtact 540atggatcgac tcggnaccaa cttgggtaat atgggcatac tggtnctggn gaaatgtttc 600cctccaatcc a 611 518 435 DNA Homo sapiens misc_feature (1)...(435) n = A,T,C or G 518cgaggtactt tntttttttt tttttttttt ttttaagagg aaaacccggt aatgatgtcg 60gggttgaggg ataggaggag aatgggggat aggtgtatga acatgagggt gttttctcgt 120gtgaatgagg gttttatgtt gttaatgtgg tgggtgagtg agccccattg tgttgtggta 180aatatgtaga gggagtatag ggctgtgact agtatgttga gtcctgtaag taggagagtg 240atatttgatc aggagaacgt ggttactagc acagagagtt ctcccagtag gttaatagtg 300gggggtaagg cgaggttagc gaggcttgct agaagtcatc aaaaagctat tagtgggagt 360agagtttgaa gtccttgaaa gaggattatg atgccactgt gaatgccttc ctagtttgag 420tttgctagcc cgcgt 435 519 407 DNA Homo sapiens misc_feature (1)...(407) n = A,T,C or G 519actttntttt tttttttttt tttttttttt ncagctttgc aaccatactc cccccggaac 60ccaaagactt tggtttcccg gaagctgccc ggcgggtcat gggaataacg ccgccgcatc 120gccggtcggc atcgtttatg gtcggaacta cnacggtntn tgatcgtntt cnaacctccg 180actttcgttc ttgattaatg aaaacattct tggcaaatgc tttcgctctg gtccgtnttg 240cgccggtcca anaatttcac ctctagcggc gcaatacnaa tgcccccggc cgtccctctt 300aatcatggcc tcagttccga aaaccaacaa aataaaaccg cggtcctatt ccattatgcc 360tagctgcggt atccaggcgg tccccggtac ctnggccgng accacgc 407 520 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 520accttctggg gcatacaaca tggcagcagg gcctcgggaa gaggggtagg aggaccgagc 60agcattctct gtagaggaag acaggaaagg agaccctctt ggcacacatt tatggagggt 120tgtccctgaa gagaagggca ggtgggagag gttccctgtt acttaagaga aggcaccagt 180ggcaaagagc acaatgaaga ggatgatgat aaaaacaatc acgcagataa ggacaatcat 240cttcacgttc ttccaccaga attttcgagc caccttctgc gatgtcgtct tgaagtgctc 300agatgtggct tccagatcct ctgtcttgtt gcggagatgt tccaagtttt ccccccgggc 360caggatccgc tccacattct gggtcataat attcttaact ccctccacct cactttgcag 420gttccgcaca cgatcatttc cttcaccttc actggcttnc tncatgtctc aaagcaccca 480gccggcagta agtgaatcgc ctatcggntt cttccaggng ggcctanttn anttctggtg 540gtcaactttc cccgcgtact tgggcggacc ccctaagggg aattcactgg cggccgtctt 600tggatccacc cgn 613 521 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 521actgcagtaa aagctttaac aggtggaatt gcccacttat tcaaacagaa taaggttgtt 60catgtcaatg gatatggaaa gataactggc aaaaatcaag tcactgctac gaaagctgat 120ggcggcactc aggttattga tacaaagaac attcttatag ccacgggttc agaagttact 180ccttttcctg gaatcacgat agatgaagat acaatagtgt catctacagg tgctttatct 240ttaaaaaaag ttccagaaaa gatggttgtt attggtgcag gagtaatagg tgtagaattg 300ggttcagttt ggcaaagact tggtgcagat gtgacagcag ttgaattttt angtcatgta 360ggtggagttg gaattgatat ggagatatct aaaaactttc aacgcatcct tcaaaaacag 420gggtttaaat ttaaattgaa tacaanggta ctggtgctcc aagaagcana tggaaaaatt 480gatgttctat tgaanctctt ttgngggaaa gctgaantnt acttggatgn cctnggccgn 540acncnctagg caatccncca ctggngccnt ntttggtccn cctggtccaa ctgggnnann 600nggctn 606 522 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 522acttgcgctt actttgtagc cttcatcagg gtttgctgaa gatggcggta tataggctga 60gcaagaggtg gtgaggttga tcggggttta tcgattacag aacaggctcc tctagaggga 120tatgaagcac cgccaggtcc tttgagtttt aagctgtggc tcgtagtgtt ctggcgagca 180gttttgttga tttaactgtt gaggtttagg gctaagcata gtggggtatc taatcccagt 240ttgggtctta gctattgtgt gttcagatat gttaaagcca ctttcgtagt ctattttgtg 300tcaactggag ttttttacaa ctcangtgag ttttagcttt attggggagg gggtgatcta 360aaacactctt tacgccggct tctattgact tgggttaatc gtgtgacccg cggtggctgg 420cacgaaattg accaaccctg gggttagtat aacttaatta aactttcntt attgctnaag 480gtaatcctgg tggttnccct gggggngtng ntaggctaaa cgtttgaacc tcattctgcg 540gcctganctt ggccctttta tcgggggatt aaaaggggac tncttgaacn gggngcttct 600tggnaaatta taaaaca 617 523 608 DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 523cgaggtactt tttttttttt tttttttttt ttttggaana agtaagcctt tatttccttg 60ttttgcaaat aaaactggct aagttggttg ctttttggtg attaagtcaa aganaccaaa 120tcccatatcc tcgtccgact cctccgactc ttccttggct tcaaccttan ctggggctgc 180agcagcagca ggagcagctg tggtggtagc aaccacaggg gcagcancca caaaggcaga 240tggatcaacc aanaaggcct tgaccttttc aacaagtggg aaggngtaat ccgtctccca 300aacaaagtca ggactcgttt gtctcttcaa aaaaaaaaag cganggctcg catttggtcc 360cctttggaca ttttgcaact cttcaatggg gttncattgg tnggtgatgg tataaacctt 420tgangnacct gcccggccgg ccgtcaaang gcaaattcac ccactggcgg ccgttctatg 480gatccnaccc ggncccaact tgggtaatat ggcanactgt tcctggggga aatgtntccc 540tnaaattccc acaaanacaa nccgaaccta aangtaancn gggggccaag agggcnaccn 600ccttattg 608 524 398 DNA Homo sapiens 524ggtacaggat cctctaaaga gaccgcctgg ctgggtgctc aaaccacatg ggccgaccca 60aaagacgtca aaaccaagag ctgctcagga ggcactaaat gttgacggtc ttggccggct 120tcacatcctc aatttcagca gacagccagc ggtaagtgcg atgacgccgc agcacctcaa 180tggccttgag ttccagtggt gttgcctgaa taccaaggtc ttctaagcca ggcaggtgag 240gcaatttcat gtctgtgatg tgcatccgct ccactttatc ccttgttatc cagggctcaa 300atgggcttat ttcaaagact cttgctaccc atcgataggc aaaaagcggc aaggggaatg 360ggaggaacaa tctgtgagcc acaacaaaga tgtacctg 398 525 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 525actgttcctg ttggcccgag tggagactgg tgttctcaaa cccggtatgg tggtcacctt 60tgctccagtc aacgttacaa cggaagtaaa atctgtcgaa atgcaccatg aagctttgag 120tgaagctctt cctggggaca atgtgggctt caatgtcaag aatgtgtctg tcaaggatgt 180tcgtcgtggc aaccgttgct ggtgacagca aaaatgaccc accaatggaa gcagctggct 240tcactgctca ggtgattatc ctgaaccatc caggccaaat aagcgccggc tatgcccctg 300tattggattg ccacacggct cacattgcat gcaagtttgc tgagctgaag gaaaagattg 360atcgccgttc tggtaaaaag ctggaaaatg gccctaaatt cttgaaatct ggtgatgctg 420ccattggtga tatgggtcct ggcaagccca tgtgtgtttg agagcttctc aaactattca 480ccttgggtcc tttgctgtcg tgatatgaaa aaacagtgcg ggggtgtatc aaacatggac 540aaaagnttnt tgacttgcag gtaccaattt nccaaaacta aaaggtnaan aaatttncca 600aaccgcc 607 526 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,C or G 526cgaggtacgc gggggaagct ctgtttggtg ctttggatcc atttccatcg gtccttacag 60ccgctcgtca gactccagca gccaagatgg tgaagcagat cgagagcaag actgcttttc 120aggaagcctt ggacgctgca ggtgataaac ttgtagtagt tgacttctca gccacgtggt 180gtgggccttg caaaatgatc aagcctttct ttcattccct ctctgaaaag tattccaacg 240tgatattcct tgaagtagat gtggatgact gtcaggatgt tgcttcagag tgtgaagtca 300aatgcatgcc aacattccag ttttttaaga agggacaaaa ggtgggtgaa ttttctggag 360ccaataagga aaagcttgaa gccaccatta atgaattagt ctaatcatgt tttctgaaaa 420tataaccagc ccattggcta tttaaaactt gtaatttttt taatttacca aaatntaaaa 480tntgaagacn taacccagtt gncatctgcg tgacaatnaa acattaatgc tacactttta 540aaaaaaaaaa aaaaaaaaaa gtcctgccng cggccctcaa aggggaattc cacacctggg 600ggccgtcttt nggncccacc cgnn 624 527 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 527acagagtgac actgaacaga tcacaaagca cgagaaacat tagttctctc cctccccagc 60gtctccttcg tctccctggt tttccgatgt ccacagagtg agattgtccc taagtaactg 120catgatcaga gtgctgtctt tataagactc ttcattcagc gtatccaatt cagcaattgc 180ttcatcaaat gccgtttttg ccaggctaca ggccttttca ggagagttta gaatctcata 240gtaaaagact gagaaattta gtgccagacc aagacgaatt gggtgtgtag gctgcatttc 300tttcttacta atttcaaatg cttcctggta agcctgctgg gagttcgaca cagtggtttg 360tttgttgctc cagatgccac ttcagaaaga tcctaaaata atctcctttc attttcaagt 420agaacacctt actttctggt tgtgtagcat tgggaataaa atatttgtcc acagcttcag 480aacatcattg cagatgtcct gcagtctggc tntatctttt acggnacctc ggccgggaca 540ccctanggcg aattccacac ctggcggccg tctantggac ngctnggcca cttgggnana 600tggctactgt t 611 528 615 DNA Homo sapiens misc_feature (1)...(615) n = A,T,C or G 528ggtacttttt tttttttttt ttttttttga gacggagtct tgttcagctg cccaggctgg 60agtgcagtgg ctcgatcttc gctcactgca accaccgtct cctgggttca agcgattctc 120ctgtctcagc ctcccaagta gctgggatta caggccacca ccatcatgcc cggctaattt 180ttgtatattg gtagagacgg agtttcacta tgttgggcag gctggtcttg aactcctcac 240ctcaggtgat ccgcccgtct tggcctccca aagtgctagg attacaggcg taagccacca 300tgcctggcca gatgatgtat ttaaatatca taccaaactc tgtgtattta tataaagaaa 360gactggtaaa agacttcctn attttaaaaa aaaccaaaac ccaaaccaaa aaaaacttta 420cccttaccat tgntgcatat tgtgcagtat aaaacacaca cttattngga catganaaaa 480ccgnaagaaa gncccgggta aactggactt tgccgccttt aaaaataaaa tcnaataagn 540gccttgaggc cctttttcaa tgcaattttt taacccggac ctgccnggng gcggtaaggg 600naatccancn ctggn 615 529 352 DNA Homo sapiens misc_feature (1)...(352) n = A,T,C or G 529cgaggtactt tntttttttt tttttttttt tttttgggaa aagtcatgga ggccatgggg 60ttggcttgaa accagctttg gggggttcga ttccttcctt ttttgtctan attttatgta 120tacgggttct tcgaatgtgt ggtagggtgg ggggcatcca tatagtcact ccaggtttat 180ggagggttct tctactatta ggacttttcg cttcgaagcg aaggcttctc aaatcatgaa 240aattattaat attactgctg ttagagaaat gaatgagcct acagatgata ggatgtttca 300tgtggtgtat gcatcggggt agtccgagta acgtcggggc attcccccgc gt 352 530 769 DNA Homo sapiens misc_feature (1)...(769) n = A,T,C or G 530ggtactgcat agattaaaga aataaactgc agtaaagcca ctcgtaagga atgaacgcca 60ttgccaatga taatcctctg cacataggtg gaaatagcaa agaagtatag ttgcttcaga 120acaggtaata accaaaatga taaacaccag aaataggaag ccaaacatgt aatacatctg 180gtgtgaccaa atactattca gaatgaagaa aagttgtata aagatgcagc caaagggcaa 240aatccctccc atgataatac caggcaaggg cttcgtgtag aacgactgtt caggaatctg 300acngtggaat ctgattggtt cgaactgggt gttcaatggc atcttcttaa aaccaangta 360tgcaccaata aacgtcnnag gcacagatat gtanaccaaa gggccaatat ggcaancagt 420gtncccaaaa gaaatactgt tgganatcct ctncccagag gtcagattnt tattaagaat 480cnccccgcgt cttttttttg tttttttttt gctccacttt nnggtaaann acntttnttt 540aaaaatgttt aantctantt cctaattccc atnttctttn gctncnnnnc tgctggnggn 600ctttaaggga antcnccnnt ggnggcgtcn atganccact tgnnactggn tantagcnac 660gttcgggang ttcccncntt ctaatatccg gnagtaannc ggctttgncn cctantggnn 720cngcttttcg aacntgcctn anannntccg gaggtgtatn ttcttctnn 769 531 777 DNA Homo sapiens misc_feature (1)...(777) n = A,T,C or G 531cgaggtactt tttttttttt tttttttttt tgtttttttt tttttttctt cagctaaaac 60agcggaagag gtgatttatt atatggttgt tacactcggc cacaaataaa cacagaaata 120gtccanaatg tcacaggtcc aggacagagg accaacatgg gcattttgtt tatgagcaag 180gtgggtctna naggtgatcg gcgatcagag ggcgatgaag ttctagatcc attgagacaa 240gctctagaca gtagcatgca gtcccacaac ttgtctccaa agattcaggt ttactcacgt 300catccagcan agaatggaaa gtcaaatttc ctgaattgct atgtgtctgg gtttcatcca 360tccgacattg aagttgactt actgaanaat ggagagagaa ttgaaaaant nggacattca 420taactgnntt tcancaagga ctggtctttc tatctcttgg ncttnntttt tcttntattt 480tttttntaca tngggcctta ctttaaaaac atacntttcc nnnttacncn tggatgccaa 540tngatttcna nanatttccn agnggaatcc tttngttatt nttaaaantt gggatctntn 600gccancactt ggctaantnt taccnncttt nggaatngtc ntatgntcat tnttggaaat 660tncccccctn angnntttct ttnngngnta aaaattntta atnnttaaat tntttttcna 720anattnntca aatactaana ntnntnnggg nttanannaa tnntgtanat gggnnng 777 532 764 DNA Homo sapiens misc_feature (1)...(764) n = A,T,C or G 532actttacaag atagattgta taagaagcca aataatgaaa gcctagaaaa aactaattta 60tacttatctg aaggttacaa attagacttt taaattttct ttgtagttgg tggtgtttga 120gggttggcta gaaatgaaag cctggatttt gtgccatgtt tgtaatatag tttgttcctt 180gatcaaataa tcagagaaaa gaaacttaaa gatctttgtc tgtgaagaag aaaattatct 240ccctagttca atctgtagtg aaataagact acagaaggca ttgttttttc ctttttattt 300tntgnattat atatttttct taaatatgtt ttattgtctt ctctaagcaa aaagttctta 360ataaacatag tatttctctc tgcgtcctat ttcattagtg aagacatagt tcacctaaaa 420tggcatnctg ctctgaatct agctttttat aaatggctat gtttttgatg atatgtcaca 480ttcaaaatgg cctaattaaa tgtgttaaat gnaatggcac tcttataacc ttaaaataac 540canaattaac cctccaaaaa aanaaaaaaa aaaaaggcct tggccgacnc ntangngant 600caccnctgng gcntcatgga cncttggcca cttgngaann nggtnangnt ccggganatt 660tccccatncc aattcancgg acatagnnac cnggccnaag ngnnccantg nngnnnnnct 720tnnngaacng gccctnaacn cccggggngg tngttcnccc tcnc 764 533 773 DNA Homo sapiens misc_feature (1)...(773) n = A,T,C or G 533cgaggtactt tttttttttt ttttacagat acaattggct tttatttgtg attcatgagt 60cagggcagtt tccattctgc aaaatatagt gatagctcct actgggcaat acaacagtag 120aacagtgggt tttgtaaaat gggaatccag gaacagaaga atataaataa attgatttaa 180ataaactgat tggttaattt cagaatactt catattactt ttttctaaga gttaaagcag 240aaaggacttt cttactgtgc tgactcagac agcctggact ctcatgtttt taggaaaatt 300ttgtctgttc tgggatctac ctgcttcctc atgttcagtg tgagtatatg gcatttagca 360tgactggtcc attctggagt caccaggctt gcacctaaat gagagttgac taancatagg 420cnttaacact actgcagtac catcatttng acttcatcat catanggtat gatgncntct 480aatnttncat tatttgagtt tggcattcag ccacgagaga atattgcctt tgacaatgnt 540gcatgcaact ttaaaggttt tagatncgcc nccnggnact atttnngaaa tcgggggtcc 600cccnanttgg agtttnacct ggcngaccnn tgacnaccat taaggantgt tagantnccc 660ttgaaccccc tttacaccnt ttgnatttcc cggcntaacc ccgggcnnta agggatccnt 720tggcntnngg cccngcnatn gaagnacntt ngannacgcc tccncaccan nng 773 534 730 DNA Homo sapiens misc_feature (1)...(730) n = A,T,C or G 534acacagacaa atttatgcga ccagggcaga ggctgtagat gattcatatt tccaattggg 60agggaggact cgcttggtct tataatatcg agccaaacgg tgaatccggc tctctattag 120aatcagacgg aatttagcat ccttatcctt tctgttcctc tcaagatgct ttcgaacagc 180aactgctttc ttaattaaat ggtagagatc ttcaggaaga tcaggagcaa gtcccttaga 240cttaagaatt cttaaaattt tattgcctgt cacaaaacgt acaaattgac caggctgttg 300acggctgcct ccacgtcggt ggaataattc tgacgaatct gggagctcat ggttggttgg 360caagaaggag ctaccacaaa aacngtgctg caggtccaga agcaggagat ggccgaaaaa 420tgtcccgaag ttcaaccgag aggaaatcga ggcggccgag cttgaagaag tcccgattgt 480tcgtcaacct gtgaacagaa caaccccgga ccgcnantgc ccggtnctgg ccggacacct 540angggaatcn accctgnggc gtctangacc acttggccaa ctggganntg gaaatntccg 600ggaaagntcn tcaatcccaa ttaccgacna agaactgggc naagggtcnc atatgggcnc 660gccttnnnga nctnccctta annccccgga gggtgntggn tctcntctan nntnnngtgg 720nggnnaanag 730 535 809 DNA Homo sapiens misc_feature (1)...(809) n = A,T,C or G 535gcgtggtcng cggccgaggt accaactgca gagccaggaa aactttgaag ccttcatgaa 60ggcaatcggt ctgccggaag agctcatcca gaaggggaag gatgtcaagg gggtgtcgga 120aatcgtgcag aatgggaagc acttcaagtt caccatcacc gctgggtcca aagtgatcca 180aaacgaattc acggtggggg aggaatgtga gctggagaca atgacagggg ataaagtcaa 240gacagtggtt canttggaag gtgacaataa actggtgaca actttcaaaa acatcaagtc 300tgtgaccgaa ctcaacggng acataatcac caataccatg acattgggtg acattgtctt 360caagagaatc agcangagaa tttaaacaag tctgcatttc atattatttt antgntgtaa 420aattaatgta attaaagtga actttgttta aaaaaagann nntnntntaa atanaaaaaa 480gtncctgcct ggcggccggt caaaggccaa ttccagcnac tngnggccnt actagtgatc 540nactcgtcna acttgcgtaa nntggcatac ttgtnctngg taaatntatc cctcncatcn 600ccaaattcnn ccgagcttaa atntaaactg gggcctatag gnncactcct tttggttgcn 660ctgccnttnn acgaacttcg ncccttttat antgcccccc ganagggtng tttggctttc 720ntnntatatt ctctctctcc ttgnnggttt ttanggtngg tcatntgggn tctntanttt 780agcttngaan ntantngntn tttnttnnt 809 536 755 DNA Homo sapiens misc_feature (1)...(755) n = A,T,C or G 536actttttttt tttttttttt tttttttttt atgaggaaaa cccggtaatg atgtcggggt 60tgagggatag gaggagaatg ggggataggt gtatgaacat gagggtgttt tctcgtgtga 120atgagggttt tatgttgtta atgtggtggg tgagtgagcc ccattgtgtt gtggtaaata 180tgtagaggga gtatagggct gtgactagta tgttgagtcc tgtaagtagg agagtgatat 240ttgatcagga gaacgtggtt actagcacag agagttctcc cagtaggtta atagtggggg 300gtaaggcgag gttagcgagg cttgctagaa gtcatcaaaa agctattant gggagtanag 360tttgaagtcc ttgagagagg attatgatgc nacttgtaat gcnttcgant ttgagtttgc 420tagcngaata nnatgaggat gtantccngg gccaatatna aaatactccc cgtnaacttn 480aggggttnga taaaatgctg tctacccnng actttgccgn acaccttagg caattcanca 540ctggngccgt ctnanggncc cacttggncc acnttggnga acatggcnnc ngtcntngga 600aatgtttcnt caattcccnc ttcnaccgan tantgnaacn ggggcanaag cncccatatn 660gtccctccct tctngaactt nnccnttaaa tncccccgga gggttnatgg ctttctctnc 720taananntnt tnngnggnnt tcnataanna taann 755 537 794 DNA Homo sapiens misc_feature (1)...(794) n = A,T,C or G 537cgaggtacga aagggacaag agaaataagg cctacttcac aaagcgcctt cccccgtaaa 60tgatatcatc tcaacttagt attataccca cacccaccca agaacagggt ttgttaaaaa 120aaaaaaaaaa aaaaaaaaaa aaaaaagtac cttgactttg ttcacagcat gtagggtgat 180gagcactcac aattgttgac taaaatgctg cttttaaaac ataggaaagt agaatggttg 240agtgcaaatc catagcacaa gataaattga gctagttaag gcaaatcagg taaaatagtc 300atgattctat gtaatgtaaa ccagaaaaaa taaatgttca tgatttcaag atgttatatt 360aaagaaaaac tttaaaaatt attatatatt tatagcaaaa gttatcttaa atatgaattc 420tgttgtaatt taatgctttt gaatacagag atntaaatga agtattatct gtaaaaatgt 480atattagagt tgtgatacag agtatatttc attcanccat nttcatacta ataatatgga 540tttaaanata tcctataaat tcnaattcaa nanaaannnt gntananaan aanggnctgn 600cggcggcgca nggcaattca acaatgnggc gtctanggac nactggtcca cttgggaana 660ggcaacttnc tgggaatgat ccttcattcc canntaccna gctanttaac nggggcaaag 720ggcccnntta tgggnntngc ntntnnaant tgcccttaaa accccggngg gtgntggntc 780tttnnntttn ngnt 794 538 766 DNA Homo sapiens misc_feature (1)...(766) n = A,T,C or G 538ggtacgcggg ggaaggcctt cctttttcgt ctgggctgcc aacatgccat ccagactgag 60gaagacccgg aaacttaggg gccacgtgag ccacggccac ggccgcatag gcaagcaccg 120gaagcacccc ggcggccgcg gtaatgctgg tggtctgcat caccaccgga tcaacttcga 180caaataccac ccaggctact ttgggaaagt tggtatgaag cattaccact taaagaggaa 240ccagagcttc tgcccaactg tcaaccttga caaattgtgg actttggtca gtgaacagac 300acgggtgaat gctgctaaaa acaagactgg ggctgtccca tcattgatgt ggtgcgatcg 360gctactacaa agttctggga aagggaaagc tccaaagcaa nctgtcatcg tgaaggccaa 420atcttcacag aagagctgag gagaaaaata agantgttgg ggggcctgtg tctggtgctt 480gaagcccatt ganggagttt aattaatgct actcttttga aaaaaanann aananaaaaa 540gacctgcccg gcggcngtaa ggcaattcac cnttgngccg tctaaggacc actggccaan 600tgggaanang gcnaanntcc tgggaatngt tcntcaattc cccaattaac caanaangna 660acnngggcca nnnggcaccc ttatggntcc ctncctttng gaactngcct tttaatccnc 720cngagggtnt tgctccttnt ntttntgnnt ggggtaatna aaagtn 766 539 789 DNA Homo sapiens misc_feature (1)...(789) n = A,T,C or G 539accattggtg gccaattgat ttgatggtaa gggagggatc gttgacctcg tctgttatgt 60aaaggatgcg tagggatggg agggcgatga ggactaggat gatggcgggc aggatagttc 120agacggtttc tatttcctga gcgtctgaga tgttagtatt agttagtttt gttgtgagtg 180ttaggaaaag gcatacagga ctaggaagca gataaggaaa atgattatga gggcgtgatc 240atgaaaggtg ataagctctt ctatgatagg ggaagtagcg tcttgtagac ctacttgcgc 300tgcatgtgcc cccgcgtact tgactttctt ttntatttnt tttattnttt ttgactactt 360agaattttca caattctaat aagattgttc caagtctctc atgtgcaagc tttaaaggat 420gactcttgcc atttatgtac ctcggncgcg accacgctaa gggcaaattc agcacacttg 480cggncgttct aagtggatcc nagctcggtc caaccttgcg tatcatggca tactggtccc 540tngtgaaatg tatcccttac aatcncacac atcnancccg aanctaaann taaanctggg 600gccaataata ctactncata atgctcnctn ctgccnttca ncnggaacnt gtgcncttnt 660tatnatggca acncggaagn gtggttggcc ttcctctcta aaacntgnng gntngttgga 720aggganctct aggnnncggt ccaattggan ncgaaattnt agctntntac naaanatntt 780tttttcncg 789 540 747 DNA Homo sapiens misc_feature (1)...(747) n = A,T,C or G 540acttttaagg gcataataag ggttaacatt ctaggcagta taaacacacc ccataatgca 60agtaataggt aatctagaga tgtggacttt attgctatat gggaattaca tttaaatttg 120agggcatttt atataaagaa aaatacagac ctataaagtt tggcatattc attaagttat 180cttttaatat ttttttctag aaaacaggtg acatttgtat ctacgataaa aatttttata 240cagaacctac tgcctcaaac tgaatcccat caagaaaact agtttctatt gnattaagta 300actcaaaata aattatcact tcgaaaactt gctttccaca ctaaggtaag tcagactaga 360tgaacactcc agaattttta ctacagactg ttttaagtta gaagtgatgg caatttataa 420attgagaata tcctccctga tgccctaact ggccaaacca aaatctaaga aagcagtgac 480ncctcttact atnatgaact tctgaatang gtagggacct cctggcntan nnatgaaaan 540ncctggccga ccccctaggg aatccncact gggggcctnn anggaccnan tggccaantt 600gnnanngggn aangnncctg gnaatgtccn caattcncna atnccgncna aagtaacngg 660gcccnggggn annnnnangn ngncnnccnn nnngaannng cccttnaann ncccgngggg 720ggngggntct nnncnnnncc nnngggg 747 541 773 DNA Homo sapiens misc_feature (1)...(773) n = A,T,C or G 541cgaggtacca tgaaatacat atatttcata aggttcagtt acaaaatgga ttgtttcaaa 60tggcaatttc ttacactaac ctgattatga aaaaaagaag tctgtatcat ctgcttccaa 120gtctgttatg tccaaatata ttttaattat gcatttattt tgctactttt ataaatatta 180gagatttcac cttaaattat ttttgtaact agttctagaa catgttttcc aattattatt 240tttctaatgg agacatataa ttgacctatg tttatgcata tatgttctct acacagtgaa 300acttttttta aaaagaatag taaagaaaat gcggaagctc tggctctcca aggcaaagtc 360aaaaaaaaaa aaaaagcggg ggggaatgcg aggaacattt tattacacct cctgatttca 420ctccttgagt ttattttctc ccttggttat tggttaatgc tagaaactgn attctaagag 480agcatccttt tcaggtgacn tgataattgg aagatttgat ccttccgcga cctgnccggc 540ggccgtcnaa nggcnattcc anccactggc ggcggtctaa nggatcnact tggnccacct 600ggctaactgg caacnggtcc ngggngaaat gnatccttaa atccncactc nacccgacct 660aangaactgg ggcaagggnc accctatggn gctcngcctt cnngaantnn ccnncttaan 720aaccnggggn gntggnntct nnnnnannnn cnnnntgngg gnntaanaag ann 773 542 770 DNA Homo sapiens misc_feature (1)...(770) n = A,T,C or G 542cgaggtactt tttttttttt tttttttttt ttttttttag aattctgaat tttattagag 60aatatatcta aaatacaata tttattaagt tatgatatat tgnctgaatg gaaatatact 120ctgnatcgca actctaatta taacaatttt tacagataat acttcattta tatctctgna 180attcaaaagt cattaaatta caacagaatt catatttaag ataactttgc tataaatata 240taataatttt taaagttttt ctttaatata acatcttgaa atcatgaaca tttatttttt 300ctgggttaca ttcatagaat catgactatt ttacctgatt tgccttaact agctcaattt 360atcttggcta tggatttgca ctcaccattc tactttccta tgtttaaaag cacattttag 420tcacaattgn gagtgctcat caccctacat gctgtgacaa aggcaagggc ctgcccgggc 480ggccgtncaa anggcgaatt ccncaactgg cggcggtcca agtggancga ctcggaccaa 540ctngggaaca tggcaactgg tcccggggaa atggaaccgt acattcccca natcagccga 600ncttaggtaa acngggggcn aagggggcta cncataatgg nggtccnccc ttcatngaac 660cgngccctnn tatnatgcac cccggagggt nnttngcctc ctcntnnnnn ntcngntgtg 720gagggagtcc nggggggtnc cangggggna aaaantgccn ngncccggng 770 543 748 DNA Homo sapiens misc_feature (1)...(748) n = A,T,C or G 543accgcgggat gcccctcatt tacataaata ttatactagc atttaccatc tcacttctag 60gaatactagt atatcgctca cacctcatat cctccctact atgcctagaa ggaataatac 120tatcgctgtt cattatagct actctcataa ccctcaacac ccactccctc ttagccaata 180ttgtgcctat tgccatacta gtctttgccg ctgcgaagca gcggtgggcc tagccctact 240agtctcaatc tccaacacat atggcctaga ctacgtacat atgctaggcc atatggtaac 300tctatgttta acattttgag gaactgccaa actgttttcc aaagtgacta cactatttta 360cattcccacc ttgaaggtcc aatttctcga cattctacca acatgggtaa tggctgcttt 420ttatttagca accttaatgg gtgtgaagag atactcaatg ggaatttgat tgattcccta 480angctaatga tttggnttct ttctggctga ngccagagnt atctntttgg gaaaattatt 540naancttgnc atttaacnng cngatttatn tgatntanaa tnttntattt gganccngcc 600tttaagnaag nttaaaattn ncaatnttgg ggcttncttt tggccatgan naannttaat 660nntannanna attnnntncn annnggcnng tnaannannn nnnanaaana annnttnnna 720anaannactt tttnnnnnna cntggcgg 748 544 327 DNA Homo sapiens misc_feature (1)...(327) n = A,T,C or G 544ctttttttt tttttttttt tttttttttt tttttttttt ttggctctag agggggtaga 60ggggtgcta tagggtaaat acgggcccta tttcaaagat ttttagggga attaattctg 120gacgatggg catgaaactg tggtttgctc cacagatttc anagcattga ccgtagtata 180ccccggtcg tgtagcggtg aaagtggttt ggtttaaacg tccgggaatt gcatctgttt 240taagcctaa tgtggggaca gctcatgagt gcaagacgtc ttgtgatgta attattatac 300aatgggggc ttnaatcggg agtacct 327

Claims

1. An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.

2. An isolated nucleic acid molecule consisting of a nucleotide sequence at least 80% identical to a sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.

3. An expression vector, capable of replicating in at least one of a prokaryotic cell and eukaryotic cell, consisting of vector sequences and the nucleic acid of claim 1.

4. The expression vector of claim 3, further consisting of a transcriptional regulatory sequence operably linked to said nucleotide sequence so as to render said expression vector capable of replicating in said at least one of a prokaryotic cell and eukaryotic cell.

5. A host cell transfected with the expression vector of claim 3.

6. A probe/primer consisting of a nucleotide sequence of 30 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.

7. The probe/primer of claim 6, wherein said probe/primer is labeled with a label group.

8. The probe/primer of claim 7, wherein said label group being selected from radioisotopes, fluorescent compounds, enzymes, and enzyme co-factors.

9. A test kit for determining the phenotype of transformed cells, conprising the probe/primer of claim 7 and suitable packaging materials therefore.

10. A pharmaceutical composition comprising a nucleic acid molecule consisting of a sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.

11. An isolated nucleic acid molecule consisting of a nucleotide sequence complementary to a nucleotide sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.

12. An isolated nucleic acid molecule consisting of a nucleotide sequence complementary to a nucleotide sequence which is at least 80% identical to a sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.

13. A probe/primer consisting of a nucleotide sequence complementary to 30 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.

14. A test kit for determining the phenotype of transformed cells, comprising the probe/primer of claim 13 and suitable packaging materials therefore.

15. A pharmaceutical composition comprising a nucleic acid molecule consisting of a nucleotide sequence complementary to a nucleotide sequence selected from the group consisting of SEQ ID Nos. 8, 13, 18-19, 41, 51, and 319.