Claims
- 1. An isolated polynucleotide comprising a member selected from the group consisting of:
- (a) a polynucleotide consisting of a polynucleotide sequence that is at least 95% identical to a polynucleotide sequence encoding amino acids 2 to 265 of SEQ ID NO. 2; and
- (b) the complement of (a).
- 2. The isolated polynucleotide of claim 1 wherein said member is (a).
- 3. The isolated polynucleotide of claim 1 wherein said polynucleotide encodes a polypeptide comprising amino acids 2 to 265 of SEQ ID NO:2.
- 4. The isolated polynucleotide of claim 1 wherein said polynucleotide comprises a polynucleotide sequence that is identical to a polynucleotide sequence encoding a polypeptide comprising amino acids 1 to 265 of SEQ ID NO:2.
- 5. The isolated polynucleotide of claim 1, wherein the polynucleotide is DNA.
- 6. The isolated polynucleotide of claim 1, wherein said polynucleotide is RNA.
- 7. A method of making a recombinant vector comprising inserting the isolated polynucleotide of claim 2 into a vector, wherein said polynucleotide is DNA.
- 8. A recombinant vector comprising the polynucleotide of claim 2, wherein said polynucleotide is DNA.
- 9. A recombinant host cell comprising the polynucleotide of claim 2, wherein said polynucleotide is DNA.
- 10. A method for producing a hIMP-H1 polypeptide comprising expressing from a recombinant cell a polypeptide encoded by a polynucleotide comprising a polynucleotide sequence encoding a polypeptide comprising amino acids 2 to 265 of SEQ ID NO:2, said produced hIMP-H1 polypeptide comprising an amino acid sequence which is at least 95% identical to amino acids 2 to 265 of SEQ ID NO:2 and said polypeptide having hIMP-H1 catalytic activity.
- 11. The isolated polynucleotide of claim 1 comprising nucleotides 4 to 789 of SEQ ID NO. 1.
- 12. The isolated polynucleotide of claim 1 comprising the polynucleotide of SEQ ID NO. 1.
- 13. An isolated polynucleotide comprising a member selected from the group consisting of:
- (a) a polynucleotide consisting of a polynucleotide sequence that is at least 95% identical to a polynucleotide sequence encoding the same mature polypeptide encoded by the human cDNA in ATCC Deposit No. 75753, and
- (b) the complement of (a).
- 14. The isolated polynucleotide of claim 13, wherein the member is (a).
- 15. The isolated polynucleotide of claim 13 comprising a polynucleotide which encodes the same mature polypeptide encoded by the human cDNA in ATCC Deposit No. 75753.
- 16. The isolated polynucleotide of claim 13 wherein said polynucleotide comprises DNA having of a polynucleotide sequence that is identical to the coding portion of the human cDNA in ATCC Deposit No. 75753 which encodes a mature polypeptide.
- 17. A method of making a recombinant vector comprising inserting the isolated polynucleotide of claim 13 into a vector, wherein said polynucleotide is DNA.
- 18. A recombinant vector comprising the polynucleotide of claim 13, wherein said polynucleotide is DNA.
- 19. A recombinant host cell comprising the polynucleotide of claim 13, wherein said polynucleotide is DNA.
- 20. A method for producing a hIMP-H1 polypeptide comprising expressing from a recombinant cell polypeptide encoded by a polynucleotide comprising a polynucleotide sequence that is at least 95% identical to a polynucleotide sequence encoding the same mature polypeptide encoded by the human cDNA in ATCC Deposit No. 75753; said produced hIMP-H1 polypeptide comprising a sequence which is at least 95% identical to the mature polypeptide encoded by the human cDNA in ATCC Deposit No. 75753.
Parent Case Info
This application is a continuation in part of PCT/U.S. Pat. No. 94/10465, filed Sep. 16, 1994.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4981980 |
Giocobbe et al. |
Jan 1991 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO 9325692 |
Dec 1993 |
WOX |