Claims
- 1. An isolated polynucleotide comprising a member selected from the group consisting of:
(a) a polynucleotide encoding the polypeptide comprising amino acid 1 to amino acid 265 as set forth in SEQ ID NO:2; (b) a polynucleotide capable of hybridizing to and which is at least 70% identical to the polynucleotide of (a); and (c) a polynucleotide fragment of the polynucleotide of (a) or (b).
- 2. The polynucleotide of claim 1 wherein the polynucleotide is DNA.
- 3. The polynucleotide of claim 1 wherein the polynucleotide is RNA.
- 4. The polynucleotide of claim 1 wherein the polynucleotide is genomic DNA.
- 5. The polynucleotide of claim 2 which encodes the polypeptide comprising amino acid 1 to 265 of SEQ ID NO:2.
- 6. An isolated polynucleotide comprising a member selected from the group consisting of:
(a) a polynucleotide which encodes a mature polypeptide having the amino acid sequence expressed by the DNA contained in ATCC Deposit No. 75753; (b) a polynucleotide which encodes a mature polypeptide having the amino acid sequence expressed by the DNA contained in ATCC Deposit No. 75753; (c) a polynucleotide capable of hybridizing to and which is at least 70% identical to the polynucleotide of (a); and (d) a polynucleotide fragment of the polynucleotide of (a), (b) or (c).
- 7. The polynucleotide of claim 1 comprising the sequence as set forth in SEQ ID No. 1 from nucleotide to nucleotide 798.
- 8. The polynucleotide of claim 1 comprising the sequence as set forth in SEQ ID No. 3 from nucleotide to nucleotide 1313.
- 9. A vector containing the DNA of claim 2.
- 10. A host cell genetically engineered with the vector of claim 9.
- 11. A process for producing a polypeptide comprising: expressing from the host cell of claim 10 the polypeptide encoded by said DNA.
- 12. A process for producing cells capable of expressing a polypeptide comprising genetically engineering cells with the vector of claim 9.
- 13. A polypeptide comprising a member selected from the group consisting of (i) a polypeptide having the deduced amino acid sequence of SEQ ID NO:2 and fragments, analogs and derivatives thereof, and (ii) a polypeptide encoded by the cDNA of ATCC Deposit No. 75753 and fragments, analogs and derivatives of said polypeptide.
- 14. The polypeptide of claim 13 wherein the polypeptide comprises amino acid 1 to amino acid 265 of SEQ ID NO:2.
- 15. A compound which inhibits activation of the polypeptide of claim 13.
- 16. A method for the treatment of a patient having need to inhibit a hIMP-HI polypeptide comprising: administering to the patient a therapeutically effective amount of the compound of claim 13.
- 17. The method of claim 13 wherein said compound is a polypeptide and said therapeutically effective amount of the polypeptide is administered by providing to the patient DNA encoding said polypeptide and expressing said polypeptide in vivo.
- 18. A process for diagnosing a disease or a susceptibility to a disease related to expression of the polypeptide of claim 13 comprising:
determining a mutation in a nucleic acid sequence encoding said polypeptide.
- 19. A diagnostic process comprising:
analyzing for the presence of the polypeptide of claim 13 in a sample derived from a host.
- 20. A process for detecting compounds which inhibit the polypeptide of claim 13 comprising:
contacting a compound with a cell line expressing hIMP-h1 in the presence of inositol monophosphate; and measuring the hydrolysis of inositol monophosphate by hIMP-H1.
Parent Case Info
[0001] This application is a divisional of U.S. application Ser. No. 09/440,113, filed Nov. 15, 1999, which is a divisional of U.S. application Ser. No. 09/314,198, filed May 19, 1999, (now U.S. Pat. No. 6,403,310, issued Jun. 11, 2002), which is a divisional of U.S. application Ser. No. 09/002,072, filed Feb. 9, 1998 (now U.S. Pat. No. 5,955,339, issued Sep. 21, 1999), which is a divisional of U.S. application Ser. No. 08/461,731, filed Jun. 5, 1995 (now U.S. Pat. No. 5,716,806, issued Feb. 10, 1998), which is a continuation-in-part of International Application No. PCT/US94/10465, filed Sep. 16, 1994. Each of the above cited patents and patent applications is incorporated by reference herein.
Divisions (4)
|
Number |
Date |
Country |
Parent |
09440113 |
Nov 1999 |
US |
Child |
10732271 |
Dec 2003 |
US |
Parent |
09314198 |
May 1999 |
US |
Child |
09440113 |
Nov 1999 |
US |
Parent |
09002072 |
Feb 1998 |
US |
Child |
09314198 |
May 1999 |
US |
Parent |
08461731 |
Jun 1995 |
US |
Child |
09002072 |
Feb 1998 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
PCT/US94/10465 |
Sep 1994 |
US |
Child |
08461731 |
Jun 1995 |
US |