Claims
- 1. An isolated nucleic acid molecule comprising: a polynucleotide sequence encoding a fragment of the human inositiol monophosphatase polypeptide consisting of amino acid residues 1 to 265 of SEQ ID NO:2, wherein said fragment has inositiol monophosphatase activity.
- 2. The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule is DNA.
- 3. The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule is RNA.
- 4. The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule is single-stranded.
- 5. An isolated nucleic acid molecule comprising a polynucleotide sequence complimentary to the polynucleotide sequence of claim 4.
- 6. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide sequence comprises a heterologous polynucleotide sequence.
- 7. The isolated nucleic acid molecule of claim 6, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.
- 8. A method for making a recombinant vector comprising inserting the isolated nucleic acid molecule of claim 1, into a vector.
- 9. A recombinant vector comprising the isolated nucleic acid molecule of claim 1.
- 10. The recombinant vector of claim 9, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 11. A recombinant host cell comprising the isolated nucleic acid molecule of claim 1.
- 12. The recombinant host cell of claim 11, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 13. A method for producing a polypeptide, comprising:(a) culturing a host cell under conditions suitable to produce a polypeptide encoded by the polynucleotide of claim 1; and (b) recovering the polypeptide from the cell culture.
- 14. An isolated nucleic acid molecule comprising: a polynucleotide sequence encoding at least 30 amino acid residues of the human inositiol monophospharase polypeptide consisting of residues 1 to 265 of SEQ ID NO:2.
- 15. The isolated nucleic acid molecule of claim 4, wherein said polynucleotide sequence encodes at least 50 amino acid residues of the human inositiol monophosphatase polypeptide consisting of amino acid residues 1 to 265 of SEQ ID NO:2.
- 16. The isolated nucleic acid molecule of claim 14, wherein said nucleic acid molecule is DNA.
- 17. The isolated nucleic acid molecule of claim 14, wherein said nucleic acid molecule is RNA.
- 18. The isolated nucleic acid molecule of claim 14, wherein said nucleic acid molecule is single-stranded.
- 19. An isolated nucleic acid molecule comprising a polynucleotide sequence complimentary to the polynucleotide sequence of claim 18.
- 20. The isolated nucleic acid molecule of claim 14, wherein said polynucleotide sequence comprises a heterologous polynucleotide sequence.
- 21. The isolated nucleic acid molecule of claim 20, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.
- 22. A method for making a recombinant vector comprising inserting the isolated nucleic acid molecule of claim 21 into a vector.
- 23. A recombinant vector comprising the isolated nucleic acid molecule of claim 14.
- 24. The recombinant vector of claim 23, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 25. A recombinant host cell comprising the isolated nucleic acid molecule of claim 14.
- 26. The recombinant host cell of claim 25, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 27. A method for producing a polypeptide comprising:(a) culturing a host cell under conditions suitable to produce a polypeptide encoded by the polynucleotide of claim 14; and (b) recovering the polypeptide from the cell culture.
- 28. An isolated nucleic acid molecule comprising: a polynucleotide sequence encoding a fragment of a human inositiol monophosphatase polypeptide encoded by the human cDNA clone contained in ATCC Deposit No. 75753, wherein said polypeptide fragment has inositiol monophosphatase activity.
- 29. The isolated nucleic acid molecule of claim 28, wherein said nucleic acid molecule is DNA.
- 30. The isolated nucleic acid molecule of claim 28, wherein said nucleic acid molecule is RNA.
- 31. The isolated nucleic acid molecule of claim 28, wherein said nucleic acid molecule is single-stranded.
- 32. An isolated nucleic acid molecule comprising a polynucleotide sequence complimentary to the polynucleotida sequence of claim 31.
- 33. The isolated nucleic acid molecule of claim 28, wherein said polynucleotide sequence comprises a heterologous polynucleotide sequence.
- 34. The isolated nucleic acid molecule of claim 23, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.
- 35. A method for making a recombinant vector comprising inserting the isolated nucleic acid molecule of claim 28 into a vector.
- 36. A recombinant vector comprising the isolated nucleic acid molecule of claim 28.
- 37. The recombinant vector of claim 36, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 38. A recombinant host cell comprising the isolated nucleic acid molecule of claim 28.
- 39. The recombinant host cell of claim 38, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 40. A method for producing a polypeptide, comprising:(a) culturing a host cell under conditions suitable to produce a polypeptide encoded by the polynucleotide of claim 28; and (b) recovering the polypeptide from the cell culture.
- 41. An isolated nucleic acid molecule comprising: a polynucleotide sequence encoding at least 30 contiguous amino acid residues of a human inositol monophosphatase polypeptide encoded by the human cDNA clone contained in ATCC Deposite No. 75753.
- 42. The isolated nucleic acid molecule of claim 41, wherein said polynucleotide sequence encodes at least 50 amino acid residues of the human inositol monophosphatase polypeptide encoded by the hurnan cDNA clone contained in ATCC Deposite No. 75753.
- 43. The isolated nucleic acid molecule of claim 41, wherein said nucleic acid molecule is DNA.
- 44. The isolated nucleic acid molecule of claim 41, wherein said nucleic acid molecule is RNA.
- 45. The isolated nucleic acid molecule of claim 41, wherein said nucleic acid molecule is single-stranded.
- 46. An isolated nucleic acid molecule comprising a polynucleotide sequence complimentary to the polynucleotide sequence of claim 45.
- 47. The isolated nucleic acid molecule of claim 41, wherein said polynucleotide sequence comprises a heterologous polynucleotide sequence.
- 48. The isolated nucleic acid molecule of claim 47, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.
- 49. A method for making a recombinant vector comprising inserting the isolated nucleic acid molecule of claim 41 into a vector.
- 50. A recombinant vector comprising the isolated nucleic acid molecule of claim 41.
- 51. The recombinant vector of claim 50, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 52. A recombinant host cell comprising the isolated nucleic acid molecule of claim 41.
- 53. The recombinant host cell of claim 52, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression.
- 54. A method for producing a polypeptide, comprising:(a) culturing a host cell under conditions suitable to produce a polypeptide encoded by the polynucleotide of claim 41; and (b) recovering the polypeptide from the cell culture.
- 55. A method of using the polynucleotide of claim 1 to detect a nucleic acid molecule in a biological sample which hybridizes thereto comprising:(a) obtaining a biological sample suspected of containing said nucleic acid molecule; (b) contacting said biological sample with said polynucleotide under conditions suitable for hybridization of said polynucleotide to said nucleic acid molecule; and (c) determining the presence or absence of said nucleic acid molecule in said biological sample.
- 56. A method of using the polynucleotide of claim 5 to detect a nucleic acid molecule in a biological sample which hybridizes thereto comprising:(a) obtaining a biological sample suspected of containing said nucleic acid molecule; (b) contacting said biological sample with said polynucleotide under conditions suitable for hybridization of said polynucleotide to said nucleic acid molecule; and (c) determining the presence or absence of said nucleic acid molecule in said biological sample.
- 57. A method of using the polynucleotide of claim 14 to detect a nucleic acid molecule in a biological sample which hybridizes thereto comprising:(a) obtaining a biological sample suspected of containing said nucleic acid molecule; (b) contacting said biological sample with said polynucleotide under conditions suitable for hybridization of said polynucleotide to said nucleic acid molecule; and (c) determining the presence or absence of said nucleic acid molecule in said biological sample.
- 58. A method of using the polynucleotide of claim 19 to detect a nucleic acid molecule in a biological sample which hybridizes thereto comprising:(a) obtaining a biological sample suspected of containing said nucleic acid molecule; (b) contacting said biological sample with said polynucleotide under conditions suitable for hybridization of said polynucleotide to said nucleic acid molecule; and (c) determining the presence or absence of said nucleic acid molecule in said biological sample.
- 59. A method of using the polynucleotide of claim 28 to detect a nucleic acid molecule in a biological sample which hybridizes thereto comprising:(a) obtaining a biological sample suspected of containing said nucleic acid molecule, (b) contacting said biological sample with said polynucleotide under conditions suitable for hybridization of said polynucleotide to said nucleic acid molecule; and (c) determining the presence or absence of said nucleic acid molecule in said biological sample.
- 60. A method of using the polynucleotide of claim 33 to detect a nucleic acid molecule in a biological sample which hybridizes thereto comprising:(a) obtaining a biological sample suspected of containing said nucleic acid molecule; (b) contacting said biological sample with said polynucleotide under conditions suitable for hybridization of said polynucleotide to said nucleic acid molecule; and (c) determining the presence or absence of said nucleic acid molecule in said biological sample.
- 61. A method of using the polynucleotide of claim 41 to detect a nucleic acid molecule in a biological sample which hybridizes thereto comprising:(a) obtaining a biological sample suspected of containing said nucleic acid molecule; (b) contacting said biological sample with said polynucleotide under conditions suitable for hybridization of said polynucleotide to said nucleic acid molecule; and (c) determining the presence or absence of said nucleic acid molecule in said biological sample.
Parent Case Info
This application is a Divisional of U.S. application Ser. No. 09/002,072, filed Feb. 9, 1998 now U.S. Pat. 5,955,339, which is a divisional of U.S. application Ser. No. 08/461,731, filed Jun. 6, 1995, now U.S. Pat. No. 5,716,806, which is a continuation-in-part of U.S. application Ser. No. PCT/US94/10465 filed on Sep. 16, 1994, now abandoned.
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Number |
Name |
Date |
Kind |
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Jan 1991 |
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Foreign Referenced Citations (1)
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WO 9325692 |
Dec 1993 |
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Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
PCT/US94/10465 |
Sep 1994 |
US |
Child |
08/461731 |
|
US |