Claims
- 1. An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:
(a) a nucleotide sequence encoding a human sel-10 polypeptide having the complete amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7, or as encoded by the cDNA clone contained in ATCC Deposit No.98978; (b) a nucleotide sequence encoding a human sel-10 polypeptide having the complete amino acid sequence selected from the group consisting of SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or as encoded by the cDNA clone contained in ATCC Deposit No. 98979; and (c) a nucleotide sequence complementary to the nucleotide sequence of (a) or (b).
- 2. An isolated nucleic acid molecule comprising polynucleotide which hybridizes under stringent conditions to a polynucleotide having the nucleotide sequence in (a), (b), or (c) of claim 1.
- 3. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:3.
- 4. The nucleic acid molecule of claim 3, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 45-1928 of SEQ ID NO:1.
- 5. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10polypeptide having the complete amino acid sequence of SEQ ID NO:4.
- 6. The nucleic acid molecule of claim 5, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 150-1928 of SEQ ID NO:1.
- 7. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:5.
- 8. The nucleic acid molecule of claim 7, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 267-1928 of SEQ ID NO:1.
- 9. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:6.
- 10. The nucleic acid molecule of claim 9, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 291-1928 of SEQ ID NO:1.
- 11. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:7.
- 12. The nucleic acid molecule of claim 11, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 306-1928 of SEQ ID NO:1.
- 13. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(b) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:8.
- 14. The nucleic acid molecule of claim 13 wherein said polynucleotide molecule of 1(b) comprises the nucleotide sequence of residues 180-1949 of SEQ ID NO:2.
- 15. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(b) encodes a human sel-10polypeptide having the complete amino acid sequence of SEQ ID NO:9.
- 16. The nucleic acid molecule of claim 15, wherein said polynucleotide molecule of 1(b) comprises the nucleotide sequence of residues 270-1949 of SEQ ID NO:2.
- 17. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(b) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:10.
- 18. The nucleic acid molecule of claim 17, wherein said polynucleotide molecule of 1(b) comprises the nucleotide sequence of residues 327-1949 of SEQ ID NO:2.
- 19. A vector comprising the nucleic acid molecule of claim 1.
- 20. The vector of claim 19, wherein said nucleic acid molecule of claim 1 is operably linked to a promoter for the expression of a sel-10 polypeptide.
- 21. A host cell comprising the vector of claim 19.
- 22. The host cell of claim 21, wherein said host is a eukaryotic host.
- 23. A method of obtaining a sel-10 polypeptide comprising culturing the host cell of claim 22 and isolating said sel-10 polypeptide.
- 24. An isolated sel-10 polypeptide comprising
(a) an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7, or as encoded by the cDNA clone contained in ATCC Deposit No. 98978; (b) an amino acid sequence selected from the group consisting of SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or as encoded by the cDNA clone contained in ATCC Deposit No. 98979.
- 25. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:3.
- 26. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:4.
- 27. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:5.
- 28. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:6.
- 29. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:7.
- 30. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:8.
- 31. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:9.
- 32. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:10.
- 33. An isolated antibody that binds specifically to the sel-10 polypeptide of claim 24.
- 34. A cell line having altered Aβ processing that expresses any of the sel-10 isolated nucleic acid molecules of claim 1.
- 35. The cell line of claim 34, wherein said Aβ processing is increased.
- 36. The cell line of claim 34, wherein said Aβ processing is decreased.
- 37. The cell line of claim 34, wherein said cell line is 6myc-N-sel10/2.
- 38. The cell line of claim 34, wherein said cell line is 6myc-N-sel10/6.
- 39. A method for the identification of an agent capable of altering the ratio of Aβ1-40/Aβ1-40+Aβ1-42 produced in any of the cell lines of claims 34, 37, and 38, comprising the steps of:
(a) obtaining a test culture and a control culture of said cell line; (b) contacting said test culture with a test agent; (c) measuring the levels of Aβ1-40 and Aβ1-42 produced by said test culture of step (b) and said control culture; (d) calculating the ratio of Aβ1-40/Aβ1-40+Aβ1-42 for said test culture and said control culture from the levels of Aβ1-40 and Aβ1-42 measured in step (c); and (e) comparing the ratio of Aβ1-40/Aβ1-40+Aβ1-42 measured for said test culture and said control culture in step (d); whereby a determination that the ratio of Aβ1-40/Aβ1-40+Aβ1-42 for said test culture is higher or lower than ratio of Aβ1-40/Aβ1-40+Aβ1-42 for said control culture indicates that said test agent has altered the ratio of Aβ1-40/Aβ1-40+Aβ1-42.
- 40. The method of claim 39, wherein said ratio of Aβ1-40/Aβ1-40+Aβ1-42 is increased by said test agent.
- 41. The method of claim 39, wherein said ratio of Aβ1-40/Aβ1-40+Aβ1-42 is decreased by said test agent.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. Ser. No. 09/213,888, filed Dec. 17, 1998, which claims the benefit of the following provisional application: U.S. Ser. No. 60/068,243, filed 19 Dec. 1997, under 35 USC 119(e)(1).
Provisional Applications (1)
|
Number |
Date |
Country |
|
60068243 |
Dec 1997 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09213888 |
Dec 1998 |
US |
Child |
10653517 |
Sep 2003 |
US |